LOCUS       DATCZV010000001         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_317527, whole genome shotgun sequence.
ACCESSION   DATCZV010000001 DATCZV010000000
VERSION     DATCZV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317527"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VIW27_00005"
     CDS             <1..147
                     /locus_tag="VIW27_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5738067.1"
                     /translation="PAAGQSVFHFHMHVLPRRDGDELKLNWGLQPGDMDAIGVLAERI
                     RENL"
     gene            complement(182..1228)
                     /locus_tag="VIW27_00010"
     CDS             complement(182..1228)
                     /locus_tag="VIW27_00010"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5738068.1"
                     /translation="MSSRKSTRGALAFERLDDSRFGVLLDFGDVGVERAVALCSAEAE
                     RLRACFYAAGGLLVMRGLQDIRQRPSALVELSRLFGPEVENYRETLTSARFFHDSIAE
                     ILVLSNAPPCNHPPPPRLAAGSESQVRFPCQVNWHTDQSYRRPPPDITLLLGLELPPP
                     GQGCTLYADCTAAFVALAPAKQRYLQTLEGIHAPSWIGRSRAAVVNGEPLRELLPHQL
                     PQRHPLVRHHPVTGKPALYICEEKQLDYVDGPVVGLTPGPHGEGAALIRELLGHATRE
                     EFVYRHRWRPGDLVVGDNRNLLHCATWYDAERYTRLMWRTTVMGNPGSEYDGEQKSWI
                     PRDGSALMAGMDGA"
     gene            1358..2044
                     /locus_tag="VIW27_00015"
     CDS             1358..2044
                     /locus_tag="VIW27_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5738069.1"
                     /translation="MAVFDAWPAMQLELISTKRCPFVQRSVITLNYKGIEHQMVFVDL
                     DDPPQWFQAISPLGRVPVLRVDGKTVIFESAVINEFLDDVTPPSLHPADPLQRALNKS
                     WIEFGGTCCSLTFQLMVAPDRQSFDDTVEQLATNLSQVEAVFGAGPYFNGAEFSLIDA
                     AYAPIFIRLDVFSELLDLQILDELPKMRAWASKLLAMPAVQSARVAELPDLFRDLITS
                     RNAYAKNRLH"
     gene            2182..2385
                     /locus_tag="VIW27_00020"
     CDS             2182..2385
                     /locus_tag="VIW27_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738070.1"
                     /translation="MLDWISKKKEQKRREEREAALKRSLSEKVAQYQEQQDDPADDHT
                     ELFINGKKVRVAKEQIDLSDPDS"
BASE COUNT          533 a          830 c          806 g          477 t
ORIGIN      
        1 cccgcggccg ggcaatcggt gtttcatttt cacatgcacg tgctgccaag gcgcgatggc
       61 gacgaactga agctcaactg gggcctgcaa cccggcgaca tggacgccat cggcgtgctc
      121 gccgagcgaa ttcgcgaaaa cctctgatca ccaccacagc gaggaggccg gcgacggttg
      181 ctcaagcgcc gtccatgcca gccatcagcg cactgccgtc gcgcggaatc caggattttt
      241 gttcgccgtc gtactcgctg ccggggttgc ccatcaccgt ggtgcgccac atcaggcggg
      301 tgtaacgctc agcgtcgtac caggtcgcgc agtgcagcag gttgcggttg tcgccgacta
      361 ccaggtcgcc ggggcgccag cggtgccgat agacgaattc ctcgcgtgtc gcgtggccga
      421 gcagttcgcg aatcagcgcc gcaccctcgc cgtgcggccc gggtgtcaat ccgacaaccg
      481 ggccgtcgac gtaatccagc tgcttttcct cgcagatata caacgcgggc ttgccggtca
      541 ccggatggtg gcgcaccagc ggatgacgtt gcggcaactg gtgcggcaac agctcgcgta
      601 gcggttcacc gtttaccacc gcggcccgtg agcggccgat ccagctcggc gcatgaatgc
      661 cctcgagggt ttgcaggtaa cgctgttttg cgggggcgag cgccacgaac gcggcagtgc
      721 aatcggcata cagcgtgcaa ccctgtccgg gcggcggcag ctccagcccc agcaacagcg
      781 tgatgtcggg cggcggccgc cggtagctct gatcggtatg ccagttgacc tggcagggaa
      841 acctcacctg cgactcgcta cccgcggcga ggcgtggcgg cggtgggtga ttacacggcg
      901 gcgcattcga caataccagg atttcggcga tcgaatcgtg aaaaaagcgc gcactggtca
      961 acgtctcgcg gtaattctcg acctcgggac cgaacaggcg gctcagctcg accagtgccg
     1021 atgggcgctg ccgaatatcc tgcaggccgc gcatcaccag caatccgccg gcggcgtaga
     1081 agcacgctcg caaccgctcg gcttcggccg agcatagcgc gacggctcgc tcgacgccga
     1141 catcgccgaa atcgagcagg acgccgaagc ggctgtcgtc gaggcgctcg aatgcgagtg
     1201 cgccgcgggt ggattttcga ctgctcatcg aggcgttact aagttagcgg gcgaatctgg
     1261 ccagaaatcc caaagatatc attaattcgg cagccgacaa gcgcttgtcg acgaccgccc
     1321 cggccgccga tggtgagccg gcggcgagcc gcgtataatg gcagttttcg acgcgtggcc
     1381 ggcgatgcaa ctggaactca tcagcaccaa gcgctgcccg ttcgtacagc gctcggtaat
     1441 tacgctcaat tacaaaggaa tagaacacca aatggtgttc gtcgatctcg acgatccgcc
     1501 gcagtggttc caggcgatat cgccgctggg ccgggtgccg gtattgcggg tcgacggtaa
     1561 aacggtgatc ttcgagtcgg cggtgatcaa cgaattcctc gacgacgtga cgcctccgag
     1621 cctgcacccg gccgacccgt tgcagcgcgc actcaacaag agctggatag agttcggcgg
     1681 cacctgctgc agcctgactt tccagctgat ggtcgcgccc gacaggcaaa gcttcgacga
     1741 taccgtcgag caactggcca ccaacctgtc gcaggtcgaa gcggtgtttg gcgcaggccc
     1801 ctatttcaac ggcgccgaat tctcgctgat cgacgcggcc tatgcgccga tcttcattcg
     1861 cctcgatgtg ttctccgaac tgctcgacct gcaaatcctc gacgagctgc caaaaatgcg
     1921 cgcctgggcg agcaagctgc tggcgatgcc ggcagtgcag tcggcacgcg tggccgaatt
     1981 gccggacctg tttcgcgatc tgatcacgag tcgcaacgca tacgcgaaaa atcgtctaca
     2041 ctagaataat tgaagacaac cgtcgagagc tgaaaagtgt ggagcttttc acctattggc
     2101 ggctgacttt catgctaatc tgcgacgctg agtgataatt tgcaacgacc ggcctgaccg
     2161 aaccaactga tagccttatt catgcttgac tggatatcca agaaaaaaga gcagaaacgc
     2221 cgcgaggagc gcgaagcagc gctcaagcgg tcgctttccg aaaaggtcgc gcagtaccag
     2281 gaacagcagg acgaccccgc cgacgaccat accgagctgt tcatcaacgg caagaaggtt
     2341 cgtgtcgcca aagaacagat cgacctgtcc gatcccgaca gctgagcttc gataaccgcc
     2401 ccggcggtag cggatttctc cccggccggg gagtttcgcc gtagctaccg cagcgagtat
     2461 ttgaatctgc cggccccgcg gcgcgctggc gcgctctgcg taacgctact ggcaattcgg
     2521 caatctcgtc cccatctagt aacattccaa gctgtcacaa accggattcg agatgacgcc
     2581 ccgattcgat aactcctatg cgcgcctgcc ggagcgcttc tatgttcgcc aggcgccggt
     2641 gccggt
//
LOCUS       DATCZV010000002         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_687942, whole genome shotgun sequence.
ACCESSION   DATCZV010000002 DATCZV010000000
VERSION     DATCZV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687942"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1119
                     /gene="trmB"
                     /locus_tag="VIW27_00025"
     CDS             430..1119
                     /gene="trmB"
                     /locus_tag="VIW27_00025"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY5738071.1"
                     /translation="MTASDPKSRSIRSFVLRTGRITPAQKQALETLWPAWGIDFRPAE
                     LALPAGFEALNLEIGIGNGDALLEMASRDPRSLYIGLEVHLPGIGRCLNGIESQDLDN
                     VRLIRHDAIEVLEQMIAPSSVDRVLLFFPDPWPKKRHHKRRIVNLRLRDLLYRVLKPG
                     GEFHAATDWEEYAEWIAAEFLDDSRFVNRGDADGYCETPVYRPRTRFERRGLALGHPV
                     RDLVFAKKPVS"
     gene            complement(1100..>2051)
                     /locus_tag="VIW27_00030"
     CDS             complement(1100..>2051)
                     /locus_tag="VIW27_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5738072.1"
                     /translation="LCSPEHLRDFVDKYGLTIQKGLRVFSEVLSSAQAGIAEIVIPPG
                     SALIGKSALDVWMRKTYGLAMVALHRDGETMMEGEHIREIPLEAGDTILAHTTWRALA
                     YLERDRNFVIVTSEYPREETRPNKVGWATSFFSLALFLVIFTDLRLSVALMTGAIGMV
                     ISGVLKIEEAYESVSWSTVFLLASLIPLGLAVETSGTAKWIAEQTLLVVGDMPIWVIQ
                     SAVALLATFFTLVMSNVGATVLLVPLAVNIAIGADANPAVFALTVALATSNSFLIPTH
                     QVNALIKGPGGYRVPDFMRAGTIMSILFIVVMMVVMTLVF"
BASE COUNT          421 a          637 c          643 g          350 t
ORIGIN      
        1 gatcacaaaa aatttgagta tccagtcaca taaatctgtg ttttcgcagt tgttatgtaa
       61 cctgcgtcac aaaaaccgcc gtggaaatcg ggatgaacaa cgaaaattag ccgataaacg
      121 gtacatcggg agaccgcccg cggtccgcca ggcgggggga cgccgatgct gccgcggtgc
      181 cgaatcacgc tgccgggtgg ttcaaaggca tgcctcgtcg ctggcggctg ccgccgaggg
      241 ggggcgggga aggctcgcaa cgatacccgc gcccgtggcg cgattgccgg ccgcatgccg
      301 cggcttctgc cggcgaccta aaactgttat ctttgagcca ccgttggcgg cagatggccc
      361 gacccgcaaa cggcaccgcg cgcagcaatg cgcaatccac cggccagcaa tcgagacctg
      421 ccccgccgca tgactgccag cgacccgaaa tcaagatcga tcagaagctt cgttctgcga
      481 accgggcgaa ttacgccggc gcaaaagcag gcgctggaga cgctgtggcc ggcctggggt
      541 atcgattttc gccccgcaga gctggcattg ccggccggat tcgaagccct gaatctggaa
      601 atcggtatcg gcaacggcga tgcgctgctg gagatggcgt cgcgggaccc gcgaagcctg
      661 tatatcggtc tcgaggtgca cctgccgggc atcggccgct gcctgaacgg tatcgagagc
      721 caggatctcg acaatgtgcg cctgatccgg cacgacgcga tcgaggttct cgagcaaatg
      781 atcgcgccgt cgtcggtcga ccgggtactg ctgtttttcc ccgacccgtg gccgaagaaa
      841 cgacaccaca agcgccgcat cgtcaacctt cgccttcgcg acctgttata ccgggtgttg
      901 aagcccggcg gcgaattcca tgcggctacc gactgggaag agtatgccga gtggatagcc
      961 gccgagtttc tcgacgattc ccgcttcgtc aaccgcggcg acgccgacgg ctactgcgag
     1021 acgcccgttt accggccgcg aacccgcttc gaacgtcgcg ggctggcgct tgggcacccg
     1081 gttcgcgacc tggtattcgc taaaaaacca gtgtcataac caccatcatg acgacgatga
     1141 acagaatgct catgatggtc ccggcgcgca tgaagtcggg cacccggtag ccgcccgggc
     1201 ccttgatcag cgcgttgacc tggtgggtcg gaatcagaaa cgaattggag gtcgcgagcg
     1261 ccaccgtcag cgcgaatacc gccgggttgg cgtcggcgcc gatggcgatg ttgaccgcca
     1321 gcggcaccag tagcacggtg gcgccgacat tcgacatcac caacgtgaaa aaagtcgcca
     1381 gcagcgcgac cgcggactga atgacccata tcggcatgtc gccgacgacc agcagggttt
     1441 gctcggcgat ccacttcgcg gtgccgctgg tctcgaccgc gagccccagc ggaatcaggc
     1501 tggcgagcag gaatacggta ctccaggata ccgattcgta ggcttcctcg atcttgagga
     1561 cgccggaaat caccatgccg atggcgccgg tcatcaacgc gaccgacagg cgcaggtcgg
     1621 taaatatcac caggaacagg gccagcgaaa aaaaactggt cgcccagccg accttgttgg
     1681 ggcgggtttc ctcacgcggg tactcggaag tcacgatcac gaaattgcgg tcgcgctcga
     1741 ggtatgcgag agccctccag gtggtgtgcg cgagtatcgt atcgccggct tccaacggga
     1801 tttcgcgaat atgctcgcct tccatcatgg tctcgccatc gcggtgcagg gctaccatcg
     1861 ccaggccgta ggtcttgcgc atccagacgt cgagcgccga cttgccgatc agcgccgagc
     1921 cgggcgggat aacgatttcg gcgatgcccg cctgcgccga ggacagcacc tcggaaaaca
     1981 cgcgcaaacc cttctgaatc gtcaggccgt acttgtcgac gaaatcgcgc aggtgctcgg
     2041 gcgagcagag g
//
LOCUS       DATCZV010000003         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_740874, whole genome shotgun sequence.
ACCESSION   DATCZV010000003 DATCZV010000000
VERSION     DATCZV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740874"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VIW27_00035"
     CDS             <1..223
                     /locus_tag="VIW27_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5738073.1"
                     /translation="LHARRITAPAIFGEHIVVADFDGYLHWFDRDDGELAGRIRFSED
                     RSYVQPLVWQDSVLTLDMNGLLVSVSPIR"
     gene            220..1563
                     /gene="der"
                     /locus_tag="VIW27_00040"
     CDS             220..1563
                     /gene="der"
                     /locus_tag="VIW27_00040"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY5738074.1"
                     /translation="MIPVIALIGRPNVGKSTLFNVFTRSRDAIVADQPGLTRDRQYGV
                     GERGGKTFIVVDTGGLSGDRRDLDDLMARQTRVAIDEADEILLLVDARDGLSSGDRAI
                     AEMVRKSGKSCQLVVNKVDGADPDQVRADFYALGFEHLHCISAAHRKGTGALLDLLLA
                     DYPRQQQDATSAEGPLVAVIGRPNVGKSTLINRLLGEERLVAFDEPGTTRDTIQVSFE
                     RHGKSYTLIDTAGVRRRGKVRQAVEKFSVIKALEAIERSQVVIVVIDAREGLTDQDLT
                     LLGYVLDAGRALVIAINKWDGLDSAQRDENRARLERRLAFVPFAAQHFISARHGTGVG
                     ELMASVDQAYASACRDLNTRLLTDLLEEAVFKHSPPLHHGRRIKLRYAHQGGRNPPVI
                     VIHGNQTQHLPEAYRRYLSNFFMTRLKMKGTPLRIELKSSSNPFAGKKGAKRRKK"
     gene            1648..>2031
                     /locus_tag="VIW27_00045"
     CDS             1648..>2031
                     /locus_tag="VIW27_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5738075.1"
                     /translation="MRILVTNAKGGCGKTTIATNIASHYARLGKNVRLFDHDAQGSSL
                     DWINRRPDDAEPIAGVDASKNMDHRLTRSWQLRVPPETEVAIIDSPAGADITELAALF
                     QPHDSVIIPVLPSPIDIHATAHFIKA"
BASE COUNT          405 a          633 c          630 g          363 t
ORIGIN      
        1 gctgcatgcg cggcgcatca ccgcgccggc aatattcggc gagcatatcg tggtcgcgga
       61 tttcgacggt tacctgcact ggttcgatcg cgacgacggt gaactggccg ggcgaatccg
      121 attctccgag gaccgcagtt acgtgcagcc gctcgtctgg caggattcgg tgctgacgct
      181 cgacatgaac ggtttgctgg tgtcggtttc gccgatccga tgattccggt aatcgcgctg
      241 atcggtcggc cgaacgtcgg caagtccacg ctgttcaacg tttttacccg cagccgcgat
      301 gccatcgtcg ccgatcagcc agggctgacg cgcgatcgcc agtacggcgt cggcgagcgc
      361 ggcggtaaaa ctttcatcgt cgtcgacacc ggcggtctca gcggcgaccg ccgggatctc
      421 gacgacctga tggcgcggca gacgcgggtc gcgatcgacg aggccgacga gatactgttg
      481 ttggtcgatg cccgcgacgg tctcagcagt ggtgatcgcg cgatcgcgga aatggtgcgc
      541 aagtccggta agtcgtgcca gctggtggtc aacaaggtcg acggtgcgga cccggaccag
      601 gtgcgggccg atttttatgc gcttggcttc gagcacctgc actgcatttc ggcggcgcac
      661 cgcaagggca ctggtgcgct gctcgacctg ctgctcgccg actacccgcg tcagcagcag
      721 gatgcgacaa gcgctgaagg accgctggtc gcggtgatcg gcaggccgaa tgtcggcaag
      781 tcgacgctga tcaaccggct gctcggcgaa gagcggctgg tcgccttcga tgagccgggc
      841 acgacccgcg atacgattca ggtcagcttc gagcgccacg gcaaatccta cacgctgatc
      901 gataccgcag gcgtacgccg gcgcggcaag gttcggcagg cggtcgagaa gttcagcgtc
      961 atcaaggcgc tcgaggcgat cgagagatcg caggtggtga tcgtcgtcat cgatgcccgc
     1021 gaaggcctaa ccgaccagga cctgaccctg ctcggctatg tgctcgacgc cggccgcgcg
     1081 ctggtcatcg cgatcaacaa atgggatggg ctggacagtg cgcaacgcga cgaaaaccgc
     1141 gcccggctgg agcgccggct ggcgttcgtg ccgttcgcgg cgcagcattt catctccgcc
     1201 aggcacggta ccggtgtcgg cgagttgatg gcctcggtcg accaggccta tgcctccgcc
     1261 tgccgcgatc tgaacacgcg gctgctcacc gacctgctcg aggaagccgt cttcaagcac
     1321 agtccgccgc tgcatcatgg ccgccgcatc aagctgcgtt acgcccacca gggcggccgc
     1381 aacccgccgg tgatcgtcat tcacggtaac cagacgcagc acttacccga agcctaccgg
     1441 cgctacctta gtaatttttt catgacccgt ctaaagatga agggcacgcc gttacgcatc
     1501 gagctgaaat cgtcgagcaa cccgtttgcg ggcaaaaagg gtgctaaaag gcgaaaaaag
     1561 tgagaccgaa tagaaaaaaa cgccaataat tactaaaaag aacccctaat ccagggtaaa
     1621 tcagcctaaa cttgtctcat gggcttaatg cgaatattgg tcaccaacgc gaaaggcggc
     1681 tgtggtaaaa ccacgatcgc caccaatatt gcgtcgcatt acgcgcggct cggcaagaac
     1741 gtgcgcctgt tcgatcacga tgctcagggc tcgtcgctgg actggatcaa ccgtcgcccc
     1801 gacgacgcgg aaccgattgc cggggtcgac gcgtcgaaga acatggatca tcgcctgacg
     1861 cgctcctggc aattgcgagt gccgccggag accgaggtcg cgatcatcga ttcgcccgca
     1921 ggtgccgaca tcaccgaact cgcggccctg ttccagccgc acgactcggt gatcatcccg
     1981 gtgttaccct cgccgatcga catccacgcc acggcacatt ttatcaaggc c
//
LOCUS       DATCZV010000004         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_264646, whole genome shotgun sequence.
ACCESSION   DATCZV010000004 DATCZV010000000
VERSION     DATCZV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264646"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2095)
                     /locus_tag="VIW27_00050"
     CDS             complement(<1..>2095)
                     /locus_tag="VIW27_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEY5738076.1"
                     /translation="IEVDGVLDAGVYNAAVTPTVAIGEANPDSSYITSGGVPFVSGAT
                     LAADGAYRLQIYASDLAGNEAQLVVRFDIDRVAPAIEILGVTDGAVYAGGVTPAIDIS
                     DTNLRSTQLSLNGQPYVSATPIEAEGSYLLEAAASDAAGNTTRVELRFRIDRSAPVIA
                     IGGVVDGAHYNTGVSAIVDVDDAGPFEASATLNGEPYVSGAAIDAPGNYLLSVIASDE
                     AGNTAAETVAFVIDTSAPLISIEGIVDDGYYAAATPRIEVDEAHPNTLEITLDGAAYV
                     SQTPIHEEGGHLLRVVATDLAGNRASREVLFTVDATPPQVVVDTPLHGETLFDRNTDV
                     VGRSEAFAQVELVLGQARYASRANAAGEFEFAGIVLEQGFNELVLGAVDRAGNAGPLT
                     KVAVQVVSGGIPGEYRTPGGVLVWAPLDDDCKSQKSRKSKKSKKTKKTKKTKKTKKSK
                     KSKKSSKYSACHRHEPGEMLDHEGEPAMLAIEAALDAARRDYQLVHGANDFLAALRSQ
                     RFGTLLLLDWHKYYCDRRDQACDDRGRKHAEPKLDKQQWREIRALVASGSGLVLIKTR
                     PDNAGDLESLGGIKVRGELPEVTAIDLLGEPLGDAGRVAYAGRALNIEPEGATPIALS
                     EPRAEVTMTATEIAAGRVVVLGFNPGAIGDRERAVALLDEVFSWVTPSTGGLAANGVA
                     EVAWLVEEVADGTPIE"
BASE COUNT          312 a          703 c          691 g          389 t
ORIGIN      
        1 gttcgatcgg ggtgccatcg gcaacctcct cgaccagcca ggcgacctcg gcgacgccgt
       61 tcgccgccag gccgccggtc gacggcgtaa cccagctgaa gacctcgtcg agcagcgcca
      121 ccgcacgctc acgatcaccg atcgcgcccg ggttgaagcc gagcacgacc acccgcccgg
      181 cggcaatctc ggtggcggtc atcgtcacct cggcgcgcgg ctcagacagc gcgatcggcg
      241 tcgcgccctc gggctcgatg ttgagcgcac ggccggcgta agcgacgcgt ccggcatcgc
      301 cgagcggctc gccgagcaga tcgatcgcgg taacctcagg cagttctccc ctgaccttga
      361 ttccgcccag gctttccagg tccccggcgt tatcgggccg cgtcttgatc agcaccagcc
      421 cgctgccgct ggcgaccagc gcgcggattt cacgccattg ctgcttgtcg agcttcggct
      481 cggcgtgctt gcgtccgcgg tcgtcgcaag cctggtccct gcggtcgcag tagtacttgt
      541 gccagtcgag cagcagcagg gtaccgaatc gctggctgcg cagggcggcg aggaaatcgt
      601 tggcgccgtg caccagctgg taatcgcgcc gcgcagcatc gagcgctgcc tcgatcgcca
      661 gcatcgccgg ctcgccttcg tggtcgagca tctcgccggg ctcgtgccgg tgacaggccg
      721 agtacttgct cgatttcttc gacttcttgc tcttcttggt tttcttggtc ttcttggttt
      781 tcttggtctt cttggatttc ttcgacttgc gcgacttctg cgacttgcag tcgtcgtcga
      841 gcggtgccca gacgagcacg ccgccgggag tgcggtactc gcccggaata ccgccggaaa
      901 ccacctgcac cgccaccttg gtgagcggtc cggcattgcc ggcacggtcg acggcgccga
      961 gaaccagttc attgaagccc tgctcgagca cgatgccggc aaattcgaac tcgccggccg
     1021 cattcgcgcg gctggcgtag cgtgcctggc cgagcacgag ttcgacctgc gcgaaggctt
     1081 cactgcggcc gacgacatcg gtattgcggt cgaacagggt ttcgccatgc agcggcgtat
     1141 cgacgacgac ctgcggtggc gtcgcgtcga cggtgaaaag tacttcgcgg ctggcccggt
     1201 tgccggcgag gtcggtggcg acgacgcgca gcaaatgccc gccctcctca tgtatcggtg
     1261 tctgcgatac gtaggcggcg ccatcgagcg tgatctcgag cgtattcggg tgggcctcgt
     1321 cgacttcgat gcgcggcgtg gcggccgcgt agtagccgtc gtcgacgatg ccttcgatac
     1381 tgatcagcgg cgcgctggta tcgatgacga aggcgacggt ttcggcggcc gtgttgccgg
     1441 cctcgtcgct ggcgatcacc gacaacaggt aattgcccgg cgcatcgatc gccgcgcccg
     1501 agacataggg ctcgccgttc agcgtggcac tcgcctcgaa cgggccggca tcgtcgacgt
     1561 cgacgatcgc gctgaccccg gtattgtagt gcgcaccgtc gacgacaccg ccgatggcga
     1621 tcaccggcgc gctgcggtcg atgcggaaac ggagctcgac gcgagtggta ttgccggcgg
     1681 catcgctggc cgccgcttcc agcaagtagc tgccctcggc ttcgatcggc gtcgccgaca
     1741 cgtagggctg gccgttgaga ctcaactgcg tgcttcgcag gttggtatcg ctgatgtcga
     1801 tggccggcgt cacgccgccg gcatagaccg cgccgtcggt cacgccgagg atttcgatcg
     1861 ccggcgcgac ccggtcgata tcgaaacgga cgaccaactg tgcctcgttg ccggcgaggt
     1921 cgctggcgta gatctgcaag cggtaagcgc cgtccgcggc cagcgtcgca ccggagacga
     1981 aaggcacgcc gccgctggta atgtaactgc tgtccggatt ggcctcgccg atcgctaccg
     2041 tcggcgttac cgccgcgttg tagacacccg cgtcgagcac gccgtcgact tcgat
//
LOCUS       DATCZV010000005         9000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_370499, whole genome shotgun sequence.
ACCESSION   DATCZV010000005 DATCZV010000000
VERSION     DATCZV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370499"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VIW27_00055"
     CDS             complement(<1..1642)
                     /locus_tag="VIW27_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5738077.1"
                     /translation="MDFYQHNQRTLVVFTIAVLILLTVSRVVNLLVFGDWGTVVANSG
                     LVPPMLLRGLRFDLKLLAVILLLVAWIPALFPARALPVRIAMPALRYALLACLLLVTF
                     VVFIEFGYLSFYAVPIDLLVFGFLEDDTAAIFKTILDNAMLVMIFVLFLIAGYVITRI
                     YLRQTKPALAQTTTPWPRSRKLAVRVVGGVLLILLARGSLDTFPLQRKSASVGDDPYI
                     NSLVMNAPFNLYYAWQDKQNNASGVFASDMLKTHDIADFETLLRKAGYSDDYPLIATT
                     ATQAPANPHLPHVVFVLMEGWSSQIALEHGDDNPVLGAFGRHAQEDHFFTHLFANTFA
                     TNSTIESLLVNSPVSPLSQSVARSTSFRLSNLLPFKRNGYRTAYVSGGYASWRNHDNF
                     WPRQGFDRYIGRSEIEDLYEVEARDHPWGVYDEYLLRRVEDFIAEADEAGVPSFSFVM
                     TTSNHSPPRLPPSYTWPRLKPELYGFDPGEQIGHDLLAGYHYASDQLGHFLSRLKAGP
                     FAERVVVVATGDHPLRTLAKTNSLAGDFLRYSVPAYFYL"
     gene            complement(1931..2677)
                     /locus_tag="VIW27_00060"
     CDS             complement(1931..2677)
                     /locus_tag="VIW27_00060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5738078.1"
                     /translation="MGRLYLIRHAQSSNNQVWDGTPYHPGREPDPDITEIGHRQAESL
                     ARHLAHPETEPRQLPFAAAERADFRLTHVYCSLMTRSIVTAGYVAEACGLQLEARSDI
                     FEQHGIYHVNEHGDFEGLPGPGRDYFEQRFPRLALPDGIDGAGWWNRPYEDEDAFVER
                     VDAVVAATGRRLADSDEHVAMVAHGDFIDQFVNALMVVGRHRHNYDNHWVANWTFHNT
                     SITRIDFVDGAHNVVYLNRIDHLPNPLVTW"
     gene            complement(2680..3555)
                     /locus_tag="VIW27_00065"
     CDS             complement(2680..3555)
                     /locus_tag="VIW27_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY5738079.1"
                     /translation="MSNEVILTCAVSGGHNNQGKHPDYPITPEHIARDCIDAAKAGAA
                     IVHIHVRDPETGMRSGDPALFREVVERIRDSGSDVLINLTTSEGARFSPGEENAGVGG
                     PGTTLVQPLERLHHVEELRPDICTLDVGTFNFGETIFVNTPAHLRIMAKRIRELGVKP
                     ELEVFEPGHIMFAQRLIEEGLIEDPPMFQLCLGIPHASPATPEILSVMRGLLPEDANW
                     SAFGISRMEFPIVAQAASQGGNCRVGLEDNLYLERGEFATNLKLVERAVRIIREVGRE
                     PASAAQAAERLGIDR"
     gene            complement(3557..4354)
                     /locus_tag="VIW27_00070"
     CDS             complement(3557..4354)
                     /locus_tag="VIW27_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5738080.1"
                     /translation="MRDSDYATLRLEYPLARVLQITLDRPAAANAFNTRMAGELMQCF
                     ESLALEPGDTRCLVITGAGERAFCAGGDLKERDGMSDEAWFAQHRIYERMIRAIIDCP
                     LPLIAAVNGAAYGGGCELAAAADFVYAADSARFAMTETSLGIIPGAGGTQNLARALGE
                     RRAKELILSARRFDAAEAQCWGLVNEVFAPAQLLAETLQTAERIAANAPLAVRQAKQA
                     IHRGLQMSLGDGLAFEIEAYNRTVPTADRREGVSAFNQKRTPEFEGR"
     gene            4578..4931
                     /locus_tag="VIW27_00075"
     CDS             4578..4931
                     /locus_tag="VIW27_00075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5738081.1"
                     /translation="MPESSAPFSQVVLDDDYAFFSGLVAADFPAGKAVLGDVAAETRA
                     VLEAIGAMLEELDLRRERIVRVDVHLADLDDFDAMDAAYREFFDNRDFPARTTVESRR
                     LFGGSKVEITCQARR"
     gene            5067..6461
                     /locus_tag="VIW27_00080"
     CDS             5067..6461
                     /locus_tag="VIW27_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY5738082.1"
                     /translation="MADSWRLSATEIARRVNAGELSATEITEQSLQRMRDVNPAINAV
                     VQELPEQALDAAREVDARVASGEQAGALAGVPVTVKVNVDQRGCANTNGIRIQRELIA
                     EHDSPVVANLRRAGAIIVGRTNTPAFSMRWFTRNSLHGHTLNPRNAALTPGGSSGGAA
                     SAVAAGICALGHGTDIAGSIRYPAYACGLHGIRPSLGRVPAVNLSLPDRHIGAQLTAV
                     SGPIARSVADLRIGLEAMAAASSLDPWWVPAPLSQPAAPRRAALCVAPDGLAVAASIE
                     SALREAGRQLEEAGWQVTETDPPPMREAMQLQLLLWMSEYAYNRGEAVRREDDPDANF
                     VYAQLLEHSPEPTMLSLMEALQRRATMARAWQQFLSEYPILLCPVSAEPPFPDQLDVE
                     STDSFRRVLEAQLTQIALPLTGMPAMTVSTGGDPRAPLGVQLVAARFREDLLFDAATE
                     IERRSPAVGISDPD"
     gene            6517..6783
                     /locus_tag="VIW27_00085"
     CDS             6517..6783
                     /locus_tag="VIW27_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009314320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HEY5738083.1"
                     /translation="MATGWARDGAVQEQIDASVDDAVEQARRRIPQGESLTHCESCSE
                     AIAEARRQALPGVRLCIACQQQLDEEQNRQSGINRRGSKDSQLR"
     gene            6889..7743
                     /locus_tag="VIW27_00090"
     CDS             6889..7743
                     /locus_tag="VIW27_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738084.1"
                     /translation="MTRQFDCRQIINHRKYPIDEPGHPARTAAIEQVRASLADDGCAV
                     IRDFFSGRGLQALLAEAEARKSQAYYSPRKLCNVYLNDGDPAHPEDHPLNVFMPRSNG
                     FITADLFGEDTAARQLYYWEPLKQFLSECLDKDELFIYEDPVSNMIVNLGQSGEEFNW
                     HFDTNEFTITMLLQAAASGGVFEYVPGLRNQRDECYDAVRQVLDGDRSRVRSLQLNAG
                     DLQFFLGRFSLHRVTANTGSTDRLLLIMSFSEKPGMVGNRARIRNLYGKLTDAHTDDR
                     VRADGLVD"
     gene            7907..8848
                     /locus_tag="VIW27_00095"
     CDS             7907..8848
                     /locus_tag="VIW27_00095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5738085.1"
                     /translation="MKQILGTMTFGGQVEALAAREMLQAFFAAGHRELDTAHSYCDGH
                     TETMLGELLPGLAQDGLYLASKVNPWESDEGLQPQQVRKQFSTILERLGRDSVDLLYL
                     HQPDLDCPVEKTLEACFEIYQQGGFRDFGLSNYAAWQVAEVVETCRRHGWMEPRVYQG
                     MYNALTRDVERELFPCLRNYGISFYAYNPLAGGFLTGKHRSLEDAPDDGRFAADRGYR
                     PRYWRQDYFRVMEELQRACDAGGLTPVEVAMSWLSHHSLLDDRAGDGIILGVSKSDHL
                     LQNMAACDAAPLDASIVDILDRGWEIIKPNCFRYFRP"
     gene            complement(8862..>9000)
                     /locus_tag="VIW27_00100"
     CDS             complement(8862..>9000)
                     /locus_tag="VIW27_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY5738086.1"
                     /translation="FIASYRFDKPVMTLYRATIPFFCVLLVSVLIITYWPSLSLVLLS
                     D"
BASE COUNT         1721 a         2923 c         2827 g         1529 t
ORIGIN      
        1 gcaggtaaaa atatgccggc accgagtagc gcagaaaatc gcccgccagg ctattggtct
       61 tggccagcgt gcgcagcggg tgatcgccag tagcgacgac gacgacacgt tcggcgaacg
      121 gcccggcctt gagcctgctc agaaaatggc ccagctgatc ggaggcgtag tgatatccgg
      181 cgagcagatc gtggccgatc tgctcgccgg gatcgaaacc gtaaagctcc ggcttcagtc
      241 gcggccacgt ataagacggc ggcaggcgcg gcggagaatg attgctcgtc gtcatcacga
      301 aactgaagct cggcacgccg gcctcgtcgg cctcggcgat gaaatcctcg acgcgtcgca
      361 gcaggtactc atcgtacacg ccccacggat gatcgcgcgc ctcgacctcg tacaggtctt
      421 cgatttccga gcgtccgata tagcggtcga aaccctgccg cggccagaaa ttgtcgtggt
      481 tgcgccagct ggcataaccg ccactgacat acgcggtacg gtaaccgttg cgcttgaacg
      541 gcagcaggtt cgacagccta aagctcgtgc tcctggccac cgattgcgac aatggcgaca
      601 ccggggaatt caccagcaag gactcgatgg tcgaattggt ggcaaaggta ttggcgaaca
      661 aatgggtgaa aaaatgatcc tcctgcgcat gccgcccaaa cgctcccagc accgggttgt
      721 cgtcgccgtg ctcgagcgca atctggctag accagccttc catcaggacg aatacgacat
      781 gcggcagatg agggttggcc ggtgcctgcg tggcggtggt cgcgatcagc gggtagtcgt
      841 cggagtagcc cgctttgcgc agcagcgttt cgaaatccgc gatatcgtgc gttttcaaca
      901 tgtcgctggc aaacacgccg ctggcattat tctgtttgtc ctgccacgca taatagagat
      961 tgaaaggcgc attcatcacc agcgaattga tatagggatc gtcgccaacc gaggccgact
     1021 tgcgttgcag cggaaaggta tccagcgatc cgcgcgcgag caggatcaac agcacaccgc
     1081 cgaccacccg caccgcgagt ttgcgagatc tcggccaggg cgtggtggtc tgcgccaggg
     1141 ccggtttcgt ctggcgcaga tagatgcgcg tgatgacata gcccgcgatc aggaagagga
     1201 cgaaaatcat caccagcatg gcgttatcga gtatggtttt gaaaatggcg gcggtatcgt
     1261 cctcgagaaa gccgaacacc agcaggtcga tcggcacggc atagaaactc agataaccga
     1321 attcgataaa taccacgaac gtcactagca gcaggcaggc cagcaaagcg tagcgcaatg
     1381 ccggcatggc gatccgcacc ggcagcgcgc gcgccggaaa caaggccgga atccacgcca
     1441 ccagcagcag tatcacggcg agcagcttga gatcgaagcg caggccgcgc aacagcatcg
     1501 gcggcaccag gccgctattg gccaccaccg tcccccagtc gccgaatacc agcaggttaa
     1561 cgaccctgga aacggtcagc aggatcagga cggcaatcgt gaaaacgacc agggtacgct
     1621 gattgtgctg gtaaaaatcc accttgaatc cgactatgtc aaaaggaaat cagatgacgg
     1681 acccgagcgc gacgagtccg cagcggatta taccgtaaat ccatcgttct ctcgtggtgt
     1741 cgatgcggac cttgcgatcg cgggccgagt gcagccttat ctgccggccc ggtaagtggc
     1801 aaggcgctgc cagaggccga tgcgacccat cggtacgcgc cagcaaagca agggagcaga
     1861 gtcgatgccg gcaacaagac ttcgaccgcc cgacacctct gcgaccggca caccggcagg
     1921 acagcatcgg ttaccaggtg accagcgggt tcggcaagtg atcgattcga ttgagataaa
     1981 ccacgttatg cgcgccatcg acgaaatcga tacgcgtgat cgaggtattg tgaaacgtcc
     2041 agttggcgac ccagtggttg tcatagttgt gacggtggcg gccgacgacc atcaacgcat
     2101 tgacgaactg gtcgatgaag tcgccatgcg caaccatcgc cacgtgctcg tcgctgtcgg
     2161 ccaggcggcg gccggtcgcg gcgaccaccg cgtcgacccg ctcgacgaac gcgtcctcat
     2221 cctcataggg gcggttccac cagcccgcgc cgtcgatacc atcgggcagc gccaggcgcg
     2281 gaaaccgctg ctcgaagtaa tcccgtcccg ggccgggcaa cccctcgaaa tccccgtgct
     2341 cgttgacgtg atagattccg tgttgctcga agatatccga ccgcgcttcg agctgcagcc
     2401 cgcaggcctc ggcgacgtag ccggcggtca ctatggagcg cgtcatcagg ctgcaataga
     2461 catgcgtcag gcgaaaatcg gctcgctcgg cggcggcgaa cggcagctgc cgcggttccg
     2521 tctcgggatg cgccaggtgc cgcgccagcg actctgcctg gcggtggccg atctcggtga
     2581 tatcggggtc cggctcgcgt cccgggtgat acggtgtgcc gtcccagacc tggttgttgc
     2641 tcgattgcgc gtgccggatc aggtacagcc ggcccatggt cagcgatcga tacccaatcg
     2701 ttcggcggcc tgcgccgcgc tcgcgggctc gcgcccgacc tcgcgaatga tgcgcaccgc
     2761 gcgctccacc agtttcaggt tggtggcgaa ttcgccccgc tcgagataca ggttgtcctc
     2821 gagaccgacc cggcaattgc cgccctggct cgcggcctgg gctacgatcg ggaattccat
     2881 ccgcgaaata ccgaacgcgc tccagttggc atcttcgggc agcagcccgc gcatcacgct
     2941 gagaatttcc ggcgtcgccg gcgacgcatg cggaatgccg agacacagct gaaacatcgg
     3001 cggatcctcg atcagaccct cttcgatcag ccgctgcgcg aacatgatgt gcccgggctc
     3061 gaatacttcc agctcgggct tgacgcccag ctcgcggatg cgtttcgcca tgatgcgcag
     3121 gtgcgccggc gtgttgacga agatggtctc gccaaaattg aaggtgccga catcgagggt
     3181 acagatatcg ggccgcagtt cctcgacgtg atgcaaccgc tccagcggct gcaccagcgt
     3241 ggtcccggga ccgccgacgc cggcgttttc ttcgcccggc gaaaagcgcg cgccctcgct
     3301 ggtggtcagg ttgatcagca cgtcgctgcc ggagtcgcga atgcgttcga cgacctcgcg
     3361 aaacagcgcc gggtcgccgg agcgcatgcc ggtctcggga tcgcgcacgt ggatatgcac
     3421 gatcgcggcg ccggccttgg cggcatcgat acagtcgcgc gcaatgtgct cgggcgtgat
     3481 cgggtaatcg ggatgcttgc cctggttgtt gtggccgccg ctgacggcgc aggtcaatat
     3541 cacttcgttc gacatcttac ctcccttcga attccggtgt acgtttctgg ttgaaagcgc
     3601 taacgccttc gcgtcgatcc gcggtcggca cggtacggtt ataggcttcg atttcgaacg
     3661 ccagcccgtc gccgagcgac atctgcagac cacgatggat cgcctgcttg gcctggcgca
     3721 ccgcgagcgg cgcattggcg gcaatgcgct cggcggtctg cagggtctcg gcgagcagtt
     3781 gcgcgggcgc aaaaacctcg ttgaccagcc cccagcactg tgcctcggcg gcatcgaaac
     3841 gccgcgccga gaggatcaat tccttggctc ggcgctcacc gagcgcgcgc gccaggttct
     3901 gcgtgccgcc ggcgcccgga atgataccga gactggtttc ggtcatcgcg aagcgcgcgc
     3961 tgtccgcggc atagacgaaa tccgccgcgg cggcgagttc gcagccgccg ccgtaagcgg
     4021 cgccgttgac tgccgcgatc agcggcagcg ggcaatcgat aatcgcgcga atcatacgct
     4081 cgtaaatgcg gtgctgcgca aaccaggcct cgtcggacat gccgtcacgc tccttgaggt
     4141 cgccgccggc gcaaaacgcg cgctcaccgg cgccggtaat caccaggcag cgcgtatcac
     4201 cgggctcgag ggccagcgac tcgaaacatt gcatcagttc gccggccatc cgggtgttga
     4261 aagcgttggc ggccgcgggg cggtcgaggg tgatctgcag cactcgcgcc agcggatatt
     4321 cgagccgtag cgtggcgtag tcggaatcgc gcatacgtgg tggtattttg gtttcggctt
     4381 gggcaatata tagtacttga ataaatctcg aaggtggata taaacatgag ctccaaccag
     4441 gacgacaaga cgaccgtcaa gcacctgaca attaccttcc tggcatttgt cgtgctgggt
     4501 gtgctgctga tcatcggggc caatataatc gcctaggacg ggctaccgcc atcctcaccc
     4561 gaatacagcc aacggcggtg cccgaatcgt cggcgccgtt cagccaggtc gtgctcgacg
     4621 acgactacgc ctttttttcc ggcctggtgg cggcggattt cccggccggc aaagccgtgc
     4681 tcggtgacgt cgccgccgag acccgcgcgg tgctcgaagc gatcggcgcg atgctcgagg
     4741 aactcgacct gcgccgcgaa cgcatcgtca gggtggatgt ccacctcgcc gatctcgacg
     4801 atttcgatgc catggacgcc gcctaccgcg agttcttcga caaccgcgac tttccggccc
     4861 gcaccacggt cgaatcccgg cgcctgttcg gcggcagcaa ggtcgagatc acctgccagg
     4921 ccaggcgctg acgaggcaag cgcgccaggg cttgcggcac ttcgagccgc gccccggagc
     4981 gccgcaattt tccgactccc cggcttttta ctgccgtata atcattcccc tgattgcaaa
     5041 ccggaccacc gacacgagga caagctatgg ccgactcatg gcgattgagc gccaccgaaa
     5101 tcgcgcgccg ggtaaacgcc ggcgaactct cggcaaccga gataaccgag caaagcctgc
     5161 agcggatgcg ggacgtcaat ccggcgatca acgccgtggt ccaggagcta cccgagcagg
     5221 cgctcgacgc ggcccgcgag gtcgacgccc gcgtggcctc gggcgagcag gccggcgcgc
     5281 tcgccggggt accggtgacg gtcaaggtca atgtcgacca gcggggctgc gccaacacca
     5341 acggcattcg cattcagcgc gaactgatcg ccgagcacga cagcccggtg gtcgccaacc
     5401 tgcgccgcgc cggcgcgatc atcgtcggcc gcaccaatac gccggctttt tcgatgcgct
     5461 ggttcacgcg caactcgttg cacggtcaca cgctcaaccc gcgcaatgcc gcgctgacac
     5521 caggcggctc gtcgggcggc gccgcgtccg cggtggccgc cggcatctgc gcactcggcc
     5581 acggcaccga tatcgccggc tcgattcgtt acccggctta cgcctgcggc ctgcacggca
     5641 ttcgcccgag tctcgggcgc gtgccggcgg tcaacctgtc gctgccggat cgtcacatcg
     5701 gcgcacagct gaccgcggtc tccggcccga tcgcgcgcag cgtggcggat ctcaggatcg
     5761 gtctcgaggc gatggccgcg gccagctcgc tggatccctg gtgggtgccg gcaccgcttt
     5821 cgcagccggc ggcgccgcgg cgcgcagcgc tctgcgtcgc gcccgacggg ctggcagtcg
     5881 ccgcctcgat cgagtcggca ttgcgcgagg ccggccggca gctcgaggaa gcgggctggc
     5941 aggtcaccga gaccgacccg ccgccgatgc gcgaggcaat gcaactgcag ttgctgctgt
     6001 ggatgtcgga atacgcctac aatcgcggcg aggcggtccg gcgagaggac gatccggatg
     6061 ctaatttcgt ttatgcgcaa ctgctcgaac attcaccgga gccgaccatg ctaagcctga
     6121 tggaagcgct gcagcgccgc gctactatgg cgcgtgcctg gcagcagttc ctcagtgaat
     6181 accccatcct gctgtgcccg gtatcggccg agccgccatt ccccgaccag ctcgacgtcg
     6241 aatcgaccga ttccttccgc cgcgtgctcg aggcgcaatt gacacagatc gcgctgccgc
     6301 tgacggggat gccggcaatg acggtgtcga ccggcggcga tccgcgcgcg ccgctcggag
     6361 tgcagctggt ggccgcgcgc tttcgcgagg acctgctgtt cgacgccgcc accgagatcg
     6421 agcgccgcag tccggcggtc ggcatttccg acccggattg aagcggcgcg cgaggcaggg
     6481 caaaataacc aacgggattt ttcgggagca gcgaacatgg caaccggttg ggcgcgagac
     6541 ggcgcggttc aggaacagat cgatgccagc gtcgacgacg ccgtcgagca ggcccgccgc
     6601 cgaattccgc agggcgagag cctgacgcac tgcgaatcct gcagcgaagc gattgccgag
     6661 gcgcgccgcc aggccttgcc cggcgtgcgc ctgtgcatcg cctgccaaca gcaactcgac
     6721 gaggaacaaa accgccagtc cggcatcaac cgccgcggca gcaaggacag ccagctgcgt
     6781 tgattcaccg gtcgtgcccg gattgacgcc gccgtgcgga atattctgtt gcgaatctcg
     6841 aggccggcgg cgtatgatcc gtagcggttc cctactgaat acccggtcat gacgcggcaa
     6901 ttcgactgca ggcagatcat caaccaccgc aagtatccga tcgacgaacc gggccacccg
     6961 gcgcgcaccg ccgccatcga gcaggtccgc gcgtcgctgg ccgatgacgg ctgcgcggtg
     7021 attcgcgatt tcttcagcgg caggggcctg caagccctgc tggccgaggc cgaggcgcgc
     7081 aagagccagg cttactattc gccgcgcaaa ctgtgcaacg tatacctcaa cgacggcgat
     7141 ccagcccacc ccgaggatca tcccctcaac gtcttcatgc cgcgcagcaa cggattcatt
     7201 accgccgatc tgttcggcga ggatactgcg gcgcgccaat tgtactactg ggaaccgcta
     7261 aagcagttcc tgagcgaatg cctcgacaag gacgaactgt tcatttacga ggatccggtg
     7321 tcgaacatga tcgtcaacct cggtcaatcc ggggaggaat tcaactggca cttcgacacc
     7381 aatgaattca ccatcaccat gctgttgcag gccgccgcca gtggcggcgt tttcgagtat
     7441 gttcccgggc tgcggaacca gcgagacgag tgttacgacg cagtcaggca ggttctcgac
     7501 ggcgatcgca gccgggtgcg cagcctgcag ctgaatgccg gcgacctgca gttttttctc
     7561 gggcgtttct cgctgcaccg ggtgaccgcg aacacgggat cgaccgatcg cctgttgctg
     7621 atcatgtcgt tcagcgaaaa acccggcatg gtcggcaacc gtgcgcggat tcgcaatctc
     7681 tacggcaagc tcaccgacgc gcataccgac gatcgcgtgc gcgccgacgg actggtcgac
     7741 tgatccccgc atcacgcgtt ccgccgccct ggcttcgggc cgcgcaagcc gcggttgcaa
     7801 tcgccggcgt cgtgccacca ccggcgggtg atcgcaccgc gggactgggc tatgataagc
     7861 gctccgccgg cgccgaccgg gccggcgacg aactcgagga gcgcagatga agcaaattct
     7921 cggcaccatg acttttggcg gccaggtcga ggcactcgca gctcgcgaga tgctgcaggc
     7981 gttcttcgcc gccggccacc gcgaactcga taccgcccac tcctattgcg acgggcacac
     8041 cgaaaccatg ctcggcgaac tgttgccggg actggcgcag gacggtcttt acctcgcctc
     8101 caaggtcaat ccgtgggaga gcgacgaggg cctgcagccc cagcaggtca gaaagcaatt
     8161 ttcgaccatc ctcgagcgcc tcggcaggga cagcgtcgac ctgctctacc tgcaccagcc
     8221 cgacctcgac tgcccggtcg aaaaaactct cgaagcctgc ttcgaaatct accagcaagg
     8281 cgggtttcgc gatttcgggc tcagtaatta cgccgcctgg caggtagccg aggtcgtcga
     8341 aacctgccgc cgccacggct ggatggagcc gcgcgtctac cagggcatgt acaacgcgct
     8401 gacccgcgac gtcgagcgcg aactgtttcc gtgcctgcgc aactacggca tcagctttta
     8461 cgcctacaac ccgctcgccg gcggattcct gaccggcaag caccgctcgc tcgaggatgc
     8521 gcccgacgac ggccgcttcg cagcggaccg cggctaccgc ccgcgctact ggcggcagga
     8581 ttactttcga gtgatggagg aactgcaacg ggcctgcgac gccggcgggc tcacgcccgt
     8641 cgaggtcgcg atgagctggc tgagccacca ttcgctgctc gatgaccgcg ccggcgacgg
     8701 catcatcctc ggtgtcagca agtccgacca cctgctgcag aacatggccg cctgcgacgc
     8761 ggcgccgctg gatgcgtcga tcgtcgacat cctcgatcgc ggctgggaga tcatcaagcc
     8821 caactgtttc cgttattttc gtccctgaac ttcagctaaa gtcagtcgga cagcaacacc
     8881 aggctcagcg atggccagta ggtaatgatc agcaccgaca ccagcagcac gcagaagaac
     8941 gggatcgtcg cccggtacag cgtcatcact ggcttgtcga agcggtagct ggcgatgaac
//
LOCUS       DATCZV010000006         6772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_635192, whole genome shotgun sequence.
ACCESSION   DATCZV010000006 DATCZV010000000
VERSION     DATCZV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635192"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VIW27_00105"
     CDS             <1..394
                     /locus_tag="VIW27_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738087.1"
                     /translation="VEKPADLAQHTLLHRDESPGPAPLDWAMWLRAAEVDGVDPERGL
                     RYSMESMAIQAAVDGHGIALASNVLAEADIEAGRLVRLFDIGLRTGLDVAYYLAYAPG
                     RLRHPRVAAFRDWLLGEVARSARLAAAG"
     gene            624..1520
                     /locus_tag="VIW27_00110"
     CDS             624..1520
                     /locus_tag="VIW27_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738088.1"
                     /translation="MNLAHLRTLVTIADTHSFATAAENLYLTPSAISHQMRDLEQELG
                     VPLFDRVTRPPRLNAHGHALVEQGRDLLTRFDTLVALARSPGEIGGQLMLGCVSGVSS
                     DLIPEALASLRASHPAVLVRMEEGLSDQLANRVLRRELDAAIITELPVADPELKSLLI
                     TKEPMMMVAPPDCRLTSWREVLATYPFIRLNRNAGMGKVIDRALRAERVVVEDAMELD
                     SSEAVVGMARAGLGAGAVPLGRLQQVAAGEIVSMPFGEPQIMRRVVLIERRNNPRSDL
                     SRLVYDAVRRATRTARTAGRRS"
     gene            complement(1606..2046)
                     /locus_tag="VIW27_00115"
     CDS             complement(1606..2046)
                     /locus_tag="VIW27_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738089.1"
                     /translation="MEGLASELLEESERVEFAQRIDRIVGATESTVDIQEYTGIAVFP
                     AITLALRPASAYEEIARFHKQMADMRPMFREPTERMVIASFVYTYQSLDAAELKKFEA
                     FLRRDDTMRFNRSVVDGLGRGLGHSIDNWAQALAMIFTHREQQI"
     gene            complement(2187..2342)
                     /locus_tag="VIW27_00120"
     CDS             complement(2187..2342)
                     /locus_tag="VIW27_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738090.1"
                     /translation="MKRFVPPALLACAAIPTAAIPAADIVVGKVLAFDREAETLVLND
                     RSVWSPG"
     gene            complement(2492..3496)
                     /locus_tag="VIW27_00125"
     CDS             complement(2492..3496)
                     /locus_tag="VIW27_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY5738091.1"
                     /translation="MVIRTESRPLARVPGISGHKPSDTRHGAMTLVRFLCLALLCVRT
                     GGVVAELSQQSFDYDGETRGYQLFVPPAYDGSNGLPLLLVLHGRGGTGERMRQLTGFD
                     LRAAEHHFFVVYPDSPQRYWNYLHSVPGAPEGADDIAFLQRLIDEIGSNYSVDRQRLY
                     VAGISNGGLMAQRLVCEQNSEFAAFASVAATGYGAMPATCRARGPVDALYLHGTHDSL
                     VPWQGRRVVGPDGESQRVTMSITDSMKFWSDLNRCDPQVEVRDLPRSGRSPKTRVRVY
                     ASKSCADGAQVKLYGILGGGHNKPGADALIPPKIAGAINFDVHASDAIWSFFDRSQPR
                     "
     gene            complement(3480..3869)
                     /locus_tag="VIW27_00130"
     CDS             complement(3480..3869)
                     /locus_tag="VIW27_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY5738092.1"
                     /translation="MIVHKGGCHCGRVRFEVRAAPVINVSQCNCSICSKSGLLGLIVP
                     RERFMLLSGEDCLSEYRFDSGEARHLFCRHCGIKSFYYPRSHPDGVSINARCLDPGTV
                     EKMNVTPFDGQNWEQQYPDGHASGYSN"
     gene            complement(3976..4491)
                     /locus_tag="VIW27_00135"
     CDS             complement(3976..4491)
                     /locus_tag="VIW27_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012604434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY5738093.1"
                     /translation="MKKSILAVSSLLLSACLGMPDSITPVSNFDLQRYLGKWYEIARL
                     DHSFERGLQRVTAEYALRDDGGISVLNRGYSANDGEWKQAQGKAYPVGDVDQGYLKVS
                     FFGPFYGSYVIFELDPEYRHAFVSGPNRSYLWLLSRTPRVEPAVVERFVDEARRLGFD
                     TDELVFVEQSA"
     gene            complement(4554..5072)
                     /locus_tag="VIW27_00140"
     CDS             complement(4554..5072)
                     /locus_tag="VIW27_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5738094.1"
                     /translation="MSAAGVTIEFASAGDAHRISALSHRYVEYGLRRRYTPAVIRQLL
                     RHRSKNVVVARLDGALIGFGIMTYRRDSANLDLLAVRKPYRRRGVGRQIVDWLEKVAR
                     TAGIANVFVQVRNSNSGAIRCYQTLGYQAITEVDGYYQGREPAVILCKGIRPMTATLL
                     TARIRAAGRIVC"
     gene            complement(5198..5494)
                     /locus_tag="VIW27_00145"
     CDS             complement(5198..5494)
                     /locus_tag="VIW27_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738095.1"
                     /translation="MQQAFRHGRRAIARQRKSPDNRRIRAAAAAAARYNQRHQTGDLA
                     LRPPEKECPKPSVTYSLLISESVYKLVVAIERLIRWFCKAVLPGCKRFYWCLRW"
     gene            5723..6550
                     /locus_tag="VIW27_00150"
     CDS             5723..6550
                     /locus_tag="VIW27_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019295653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5738096.1"
                     /translation="MNDKVSTHYGSGGDLADRIAGSLRSQGRDLADLQPSDLSYIDEF
                     HLLGRRATLRLAAQLQLTAHSRVVDIGSGLGGAARTLAAEYGCHVTGIDLTPAYCEVA
                     GVMSNWVGLAARTSFQLGDATRMPFAGQQFDAAISLHAAMNIAARDRLYDEARRVLVP
                     GSRFAVFDILQGEGGEALYPAPWAREPSISHLVTREAMQQLLSGAGFDILEIHDSTAE
                     SLEWLRARSAGARPGGTPPVTSKILFGDDFDWPQMVRNQLRGLEERRIRTVSFVCVA"
BASE COUNT         1236 a         2099 c         2226 g         1211 t
ORIGIN      
        1 ggtcgaaaag cccgccgacc tggcgcaaca tacgctgctg caccgcgacg agtcgcccgg
       61 accggcgccg ttggactggg cgatgtggct acgggcggcc gaagtcgacg gtgtcgatcc
      121 cgagcgtgga ctgcgctata gcatggaaag catggcgatc caggcggcgg tcgacggtca
      181 cggcatcgcg ctggccagca atgtgctggc ggaggcagat atcgaggccg gacggctggt
      241 caggctgttc gacatcggcc tgcgcaccgg gctcgacgtc gcctattacc tcgcctacgc
      301 gcccggcagg ctgcgccatc cgcgggtcgc cgcgttccgc gactggctgc tgggagaagt
      361 cgccaggtcg gcgcggctgg ccgcggccgg ctgagccgga actcgcgctc ggcgagctgc
      421 gggcaatgga taatagcggc agggttaaac caggaactgc aggttggcgc cgaggatgcc
      481 ggcaatcatt accgcgacga gcagggtgtc tgacatgatg tgctccttga gggcttcggg
      541 attgatgacg tagaagtata gagttgatta aaattcaata aaaacgataa taattcaaaa
      601 ttatattgaa attaattcat taaatgaatc ttgctcacct gcgcacgctg gtcaccatcg
      661 ccgacaccca cagttttgcc actgccgccg aaaacctcta cctgactccg tcggcgatca
      721 gtcaccagat gcgcgatctc gaacaggaac tcggcgtgcc gctgtttgac cgcgtgacgc
      781 ggccgccgcg cctcaacgcc catggccatg cattggtaga gcagggccgc gatctgctga
      841 ctcgcttcga tacgctggtg gcgctggcgc gttcgccggg agagatcggc gggcagctga
      901 tgctcggttg tgtcagcggc gtatccagtg acctgattcc cgaggcgctg gcgagcctgc
      961 gagcctcgca tccagcggtg ctggtgcgca tggaagaggg cctttccgac cagcttgcca
     1021 accgcgtgct gcggcgcgaa ctcgatgcgg cgataattac cgagctgccg gtggccgacc
     1081 cggagcttaa atcgctgctg attaccaagg agccgatgat gatggtcgcg ccgcccgact
     1141 gtcgcctgac ttcatggcgc gaggtgctcg ccacctatcc attcattcgc ctgaaccgca
     1201 atgccggcat gggcaaggtg atcgaccgtg cgctgcgcgc cgagcgagtt gtcgtcgagg
     1261 acgccatgga actcgactcg tccgaggccg tggtcggcat ggcgcgcgcc ggcctcggtg
     1321 ccggcgcggt gccgctcggc aggttgcagc aggtcgcggc cggcgaaatc gtcagcatgc
     1381 cgttcggcga gccgcagatc atgcggcgcg tggttctgat cgagcgccgc aacaacccgc
     1441 gctccgacct gtcgcggttg gtttatgatg cggtgcgtcg ggcgacccgc acggctcgaa
     1501 ccgccgggcg gcggagttga aagccggcgg atgcgggcag cttcggcccc ggcggcataa
     1561 accgatcgtc ctggcggttg aagagccggc tcctgccggc gccgttcaga tctgctgttc
     1621 ccggtgggtg aaaatcatcg ccagagcctg tgcccagttg tcgatcgaat gcccgaggcc
     1681 gcgccccagg ccatcgacca cgctgcgatt gaagcgcatg gtgtcgtcac ggcgcagaaa
     1741 ggcttcgaat ttcttcagtt cggctgcatc gagcgactgg taggtataga cgaaggatgc
     1801 gatgaccatg cgctcggtgg gctcgcgaaa catcggccgc atgtcggcca tctgcttgtg
     1861 aaagcgggcg atttcctcgt aggcggaggc gggccgcaac gccagcgtga tcgccgggaa
     1921 taccgcaatg ccggtgtact cctggatgtc caccgtcgac tcggtggcgc cgacgatgcg
     1981 gtcgatacgc tgcgcgaatt cgactcgctc ggattcctcg agcagctcgg atgccaggcc
     2041 ttccatttgc gcaacggcct cgggcgtgcc ggcgctccgc gaatgacgtt gatttcgaga
     2101 atatgcgagt cctcgcctgc cgcggactcg cggctgaact ggacgcgggc gacggcggcg
     2161 agattcccgg gaagcgcggc agactgttac cccggcgacc agacgctgcg gtcgttcaaa
     2221 accaaggttt cggcctcgcg gtcgaacgcc agtaccttgc cgaccacgat atcggcggcc
     2281 ggtatcgccg ccgtcggaat ggcggcacag gcgagcagcg ctggcggaac aaatcgtttc
     2341 atcggtcacg ttcgaaccag ccacgccggt tacccagggg tcaccttaac gcagaaaacc
     2401 tcgcgcggta aaactcgacg ccgcgccggc cgctggtaat ggccgggata tgccgattag
     2461 ccgcgattca gcgaagcgaa acatgcgcct gtcagcgcgg ctgcgagcgg tcgaaaaacg
     2521 accagatcgc gtcactggcg tgaacatcga agttgatcgc accggcgatc ttcggcggaa
     2581 tcagtgcatc cgcccccggc ttgttgtgtc cgccgccgag aattccgtag agcttcacct
     2641 gcgcgccgtc ggcgcaggac ttggacgcat agaccctgac gcgggtcttc ggcgagcgcc
     2701 ccgagcgcgg caggtcgcgc acttcgacct gcgggtcgca gcggttgagg tccgaccaga
     2761 acttcatcga gtcggtgatc gacatcgtga cccgctgcga ttcgccgtcg ggaccgacca
     2821 cgcgccgccc ctgccagggc accaggctgt cgtgggtgcc atgcaggtag agcgcgtcga
     2881 ccggcccccg ggctctgcag gtcgcgggca tggcgccgta accggtggcg gcgaccgatg
     2941 cgaaagccgc gaactcgctg ttctgctcgc agaccaggcg ctgtgccatg aggccaccgt
     3001 tggagatccc ggcgacgtac agccgctggc ggtcgaccga gtaattgctg ccgatttcat
     3061 cgatcaggcg ctgcagaaaa gcgatatcgt cggctccctc gggggcgccc ggaacactgt
     3121 gcaggtaatt ccagtagcgc tgcgggctgt cggggtagac gacaaaaaaa tggtgctcgg
     3181 cggcgcgcag atcgaagccg gtcagctgtc gcatgcgctc gccggtgccg ccgcggccat
     3241 gcagcaccag cagcagcggc aggccgttac tgccatcgta ggccggcggc acgaaaagct
     3301 ggtaaccgcg agtttcgccg tcgtagtcaa acgactgctg cgacagctcg gcaaccacgc
     3361 cgccagtgcg cacgcaaagc agagcgagac acaggaaacg caccagggtc atcgcaccgt
     3421 gccgggtatc cgatggcttg tggccggata tccccggcac gcgggcaagt ggcctggatt
     3481 cagttcgaat aaccactggc gtgaccgtcg gggtactgct gttcccagtt ttgcccatcg
     3541 aaaggggtca cgttcatctt ttcgaccgtg cccgggtcca gacaacgcgc gttgatgctg
     3601 acgccgtcgg gatgcgagcg cgggtagtag aacgacttga tgccgcagtg tctgcagaac
     3661 aggtgcctcg cctcgccgct gtcgaagcgg tactcggaca ggcagtcctc gccggaaagc
     3721 agcatgaatc gctcccgcgg cacgatcaga ccgagcaggc cggatttgga gcagatgctg
     3781 caattgcatt gcgaaacatt gatcaccggc gccgcacgga cttcgaacct gactcgcccg
     3841 cagtggcagc cacccttatg tacgatcatc agcgctcctg ccgccggcag cggcggtcga
     3901 cgaccccggc cgttgcccgg cacgattgtt taccgatctg tattctaccg cgtatcggcg
     3961 gtatcgctag ccgggttatg cgctctgctc gacgaacacc agttcgtcgg tatcgaaacc
     4021 gagtcgccgg gcttcgtcga cgaagcgttc gacgacagcc ggttcgacgc gcggcgtgcg
     4081 cgacagcagc cacaggtagg agcgattcgg gccggagacg aaggcgtggc ggtattcggg
     4141 atccagttcg aagatcacgt aggaaccgta gaacgggccg aagaaggata ccttcaggta
     4201 gccctggtcc acatcgccga ccgggtaagc cttgccctgt gcctgtttcc attccccgtc
     4261 gttcgcggaa taaccgcggt tcagcaccga gatgccgccg tcgtcgcgca gcgcgtactc
     4321 ggcggtaacg cgctgcaggc cgcgctcgaa ggaatgatcg aggcgcgcaa tttcgtacca
     4381 cttgccgagg taccgctgca ggtcgaagtt gctgacgggc gtaatcgagt cgggcatacc
     4441 gaggcaggcc gacagtagca gcgaggacac ggcgagaata gattttttca tcggacgatt
     4501 gcaatgctcg gtaacaggat ggacggggaa tacggcaaat gtgggcgcgc ggatcagcag
     4561 acgatgcgtc ccgccgctcg aatgcgggcg gtcagcaggg tggcggtcat cggccgaatt
     4621 cccttgcaca ggatcaccgc cggctcgcgc ccctggtaat agccatcgac ctcggtgatg
     4681 gcctggtaac cgagtgtttg atagcagcga atcgcgccgc tattggaatt gcgcacctgc
     4741 acgaatacat tcgcgatccc ggcggtgcga gcgacctttt cgagccaatc gacgatttgc
     4801 ctgccaacac cgcggcggcg gtaaggcttg cgcaccgcca gcaggtcgag gttagcgctg
     4861 tcgcgccggt aagtcatgat gccgaaaccg atcagggcgc cgtcgaggcg ggccaccacc
     4921 acgtttttcg agcgatgccg cagcaattga cgaatcaccg ccggcgtgta gcgtcgccgc
     4981 aggccgtact cgacgtagcg gtgcgacagc gcgctgattc gatgggcatc gccggcgctg
     5041 gcaaactcga tggtcacgcc tgcggctgac atcaatctct gccgcgcttc gcggcctgcg
     5101 acgttcgtcg tgccccgtac accgtgttgg tgccggccgc cggtcgcgag tcaggagttg
     5161 tgatcattcg tcgccgcccg tgatccgctc gccggcgtta ccagcgcagg caccagtaaa
     5221 atcgcttgca gccgggcagc acggctttgc agaaccagcg aatcagccgc tcgatggcca
     5281 ccaccagctt gtaaaccgat tccgaaatca gcagcgaata ggtaaccgac ggcttcgggc
     5341 attctttttc tggaggtctc aaggccaagt ctccggtctg atgtcgctga ttatagcggg
     5401 cagcggctgc ggctgctgcc cgaatacgcc gattatctgg agattttcgc tgtcttgcaa
     5461 tcgcccgccg gccgtggcga aaggcctgct gcatgccatg ctagcgcagt agcggctcag
     5521 gattgagcga tgccagcggt cctccgtgcc gtcagggaat tgcgctcctg ctcgtcttgc
     5581 cgatggccga tctcggccag gtgctgccgc tcgactgcaa atcggttcgc ggcccgcggt
     5641 tccagcggat tcgttgaaat gatacctttg ccggttatcg cgggtgcgga gccggcggac
     5701 ggcaagcggc cggagcagga agatgaacga caaagtttca acccattacg gcagcggcgg
     5761 cgacctcgcc gacaggatcg cgggcagcct gcgcagccag ggcagggatc tcgccgatct
     5821 gcagccgtca gacctgtcgt acatcgacga gtttcattta ctcggccgcc gggcgaccct
     5881 gcggctggcg gcacaactgc agctcaccgc gcacagccgc gtagtcgaca tcggcagcgg
     5941 tctcggcggt gccgcgcgca cgctggccgc ggaatacggc tgtcatgtca ccggcatcga
     6001 cctgaccccg gcttattgcg aggtcgcggg cgtgatgtcg aactgggtcg ggctcgccgc
     6061 gcggacctcg ttccagctgg gggacgcgac ccgcatgcca tttgcggggc agcaattcga
     6121 cgctgcgatc agcctgcacg cggcgatgaa catcgccgcc agggacaggc tctacgacga
     6181 ggcccggcgg gtgctggtgc cgggcagccg gttcgccgtt ttcgacatat tgcagggcga
     6241 gggtggtgaa gcgctgtacc cggcgccctg ggccagggag ccgtcgatca gtcacctggt
     6301 gacgcgagaa gccatgcagc aactgctaag cggtgccggc ttcgacatac tggagattca
     6361 cgattccacc gccgaaagcc tcgaatggct gcgcgccaga tcggcgggcg cgaggccggg
     6421 cggtacgccg ccggtgacct cgaaaatcct cttcggcgac gacttcgact ggccgcagat
     6481 ggtacgcaac cagctgcgcg gtctcgagga acgcaggatt cgaaccgtca gtttcgtctg
     6541 cgtagcctga cggcggagaa gccgaggccg aagcgggcgg cgtcaatgcg ggcatcctgg
     6601 gcatttcatc caccgtgatg ctgccgtttt ccctggctct tcgatgcgca gaattgtgcg
     6661 gcaattatca ccgtgccttc atatctacga cgaactatgt caatttcccc gctgtcgaag
     6721 cgatagctca cagttgaatt gaaaggcgcg cctgttggtt ttggaaccat cg
//
LOCUS       DATCZV010000007         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_476423, whole genome shotgun sequence.
ACCESSION   DATCZV010000007 DATCZV010000000
VERSION     DATCZV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476423"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIW27_00155"
     CDS             <1..190
                     /locus_tag="VIW27_00155"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HEY5738097.1"
                     /translation="AESLGGETPLQARERRLESERLAAIDAIRGDETVLKLQRAFAAE
                     LDEASVVRIGTSNNEEVS"
     gene            187..513
                     /locus_tag="VIW27_00160"
     CDS             187..513
                     /locus_tag="VIW27_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY5738098.1"
                     /translation="MMKGGLGEMMKKAQEMQENLQKAQRELANREVTGESGGGMVKIV
                     MRGTHEVNRVTIDPSLIDEDVEMLEDLVAAAINDAVHKVEKSNQAAMQEMTAGLNLPP
                     GFKLPF"
     gene            538..1134
                     /gene="recR"
                     /locus_tag="VIW27_00165"
     CDS             538..1134
                     /gene="recR"
                     /locus_tag="VIW27_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5738099.1"
                     /translation="MSSPSLDEMISALKCMPGVGQKSAQRMALHLLERDQQGARRIAE
                     AITVALDRVRHCRRCRNFSDAELCTVCEDSSREQNTICVVETPADVLAVEASAAYRGR
                     YFVLLGRLSPLDGYGPDQLGLEDLEQQLTDGGVDEIILATSATVEGDITAQYIADFAV
                     RHGVATSRLARGIPLGGELEFVDGGTLSRALAGRQKFE"
     gene            1158..1505
                     /locus_tag="VIW27_00170"
     CDS             1158..1505
                     /locus_tag="VIW27_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY5738100.1"
                     /translation="MSESIFTRIINRELPADFVYEDDDIAAIRDINPQAPTHVLIVPK
                     VEIPTVNDIQPEQAELIGRMVLVAQRIAAEEGIAEDGYRLVINCNRHGCQEVFHLHLH
                     LIGGRQLRGISAA"
     gene            complement(1502..>2287)
                     /gene="rsgA"
                     /locus_tag="VIW27_00175"
     CDS             complement(1502..>2287)
                     /gene="rsgA"
                     /locus_tag="VIW27_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY5738101.1"
                     /translation="NLGSIVCGDRVSIESVAEEHRVAAVIERDNLLQRVDGFGHARPV
                     AANISQLVVCLAYSPEPNLFLLDQYLLSAEQQAIDACILINKYDLAGERQDPFGIERV
                     YRPLGYRVLSTSVESGEGMLEFAAMLRGRVSVLGGVSGVGKSSLTAWLLPDEEIKVAS
                     ISAANEEGRHTTRASRLYHLPGGGELIDTPGVRGFSPFVDDRLALDSGFREIRQLAAD
                     CRFHNCLHRDEPNCAVATAAAAGEVAPSRYLSYLKLLEQAKSG"
BASE COUNT          452 a          713 c          738 g          384 t
ORIGIN      
        1 cgcggagtcg ctcggcgggg aaacgccgct gcaagccagg gagcggcgcc tggagagcga
       61 acggctggcg gcgatcgatg ccattcgcgg tgacgagacg gtattgaaac tgcagcgcgc
      121 gtttgccgcc gaactcgacg aggcgagcgt ggtcaggatc ggcaccagca ataacgaaga
      181 ggttagttga tgaaaggcgg actcggcgaa atgatgaaaa aggcgcagga gatgcaagag
      241 aacctgcaaa aggcgcagcg cgagctggcc aacagggaag tcaccggaga atcgggcggc
      301 ggcatggtca agatcgtgat gcgtggtacc cacgaggtga atcgcgtgac catcgatccg
      361 agcctgatcg acgaggacgt cgaaatgctc gaggacctgg tggccgcggc gatcaacgat
      421 gccgtgcaca aggtggagaa atccaaccag gcggcgatgc aggaaatgac cgccgggctc
      481 aatttgccgc ctggtttcaa gctgccgttc tagcccgctt taccgtcaga cgaatttatg
      541 tcctcgccga gcctggatga aatgatcagt gcgctcaagt gcatgccggg cgtcggtcaa
      601 aagtcagcgc agcgcatggc cctgcacctg ctcgagcgcg accagcaggg cgcgcgccga
      661 atcgccgagg cgattaccgt cgcgctcgac cgggtccgcc attgccgacg ttgccgcaat
      721 ttttcggatg ccgaactgtg caccgtctgc gaggattcga gccgcgaaca aaataccatt
      781 tgcgtggtcg agacgccggc cgacgtgctc gcggtcgaag cctctgcagc ctaccgcggc
      841 cgctacttcg tattgctcgg ccggctgtcg ccgctggatg gctacggccc cgaccagctc
      901 ggtctcgaag acctcgaaca gcaattgacc gacggcggcg tcgacgaaat cattctcgcc
      961 accagtgcca cggtcgaggg cgatatcacg gcccagtaca tcgccgattt cgcggtgcgc
     1021 cacggcgtcg ccaccagccg cctggcgcgt ggtattccgc tcggcggcga gctcgaattc
     1081 gtcgacggcg gcaccctgtc gcgagcgctg gccggacgac agaagttcga atagcgttcc
     1141 agcacttcgg aggacccatg tcagaatcca tatttacccg catcatcaat cgcgaactgc
     1201 ccgccgattt cgtatacgaa gacgacgaca tcgccgccat tcgcgatatc aatccgcagg
     1261 cgcccacgca tgtattgatc gtgccgaagg tcgaaattcc gaccgtcaac gatatccagc
     1321 ccgagcaggc ggaactgatc ggcaggatgg tgctggtggc ccagcggatc gccgcggaag
     1381 agggcatcgc ggaggacggt tatcggctgg tcatcaactg taatcggcac ggctgccagg
     1441 aagtcttcca cctgcatttg cacctgatcg gcggccggca gttgcgcggt atttcggccg
     1501 cctagccact tttcgcctgc tccagcaatt tcaggtaaga caggtaacgg ctcggcgcga
     1561 cttcgcccgc cgcggccgcc gtggcgaccg cgcaattcgg ctcgtcacga tgcaggcagt
     1621 tgtgaaaccg acagtccgcg gccagctggc gaatctcgcg gaatcccgaa tccagcgcaa
     1681 gcctgtcgtc gacgaacgga ctgaagccgc gcaccccggg cgtatcgatc agttcgccac
     1741 cgcccggcag atggtaaagg cggctcgcac gggtagtgtg gcggccttcc tcgttggcgg
     1801 ccgagatgct cgccaccttg atctcctcgt ccggcagcag ccaggcggtc agcgacgact
     1861 tgccgacccc ggacacgccg ccgagcaccg acactcggcc gcgcagcatc gccgcgaatt
     1921 cgagcatgcc ctcacccgac tcgacgctgg tggaaagcac gcggtaaccg agcggccggt
     1981 agacgcgctc gattccgaac gggtcctgcc gttcgccggc gaggtcgtat ttgttgatca
     2041 ggatgcaggc gtcgatggcc tgctgctcgg cggataacag gtactggtcg agcaggaaca
     2101 ggttgggctc cggggagtag gcgaggcaga ccaccagctg actgatgttg gcggcaaccg
     2161 ggcgcgcgtg cccgaagccg tcgacacgct gcagcagatt atcgcgttcg atgactgccg
     2221 ccacgcggtg ttcctctgcc acggattcga tgctgacccg gtcgccgcag actatgctgc
     2281 cgagatt
//
LOCUS       DATCZV010000008         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_271, whole genome shotgun sequence.
ACCESSION   DATCZV010000008 DATCZV010000000
VERSION     DATCZV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1109)
                     /locus_tag="VIW27_00180"
     CDS             complement(15..1109)
                     /locus_tag="VIW27_00180"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY5738102.1"
                     /translation="MRYLISIPLLLLLGGCADMGYYWHSARGHLEVMDKRVAIEDLLA
                     EQSTTPELRERLTLVREIREFSVTRLDLPDNDSYLSYVELDRPYLVQNLFAAPEFSTR
                     LRQWCYPIIGCASYRGYYDETRLLAYVEELETAGLEVHVGRVSAYSTLGWFDDPVLSS
                     FINWPDYRLAGLLFHELTHQQIYIEDDTTFNESLASAVQQVGTELWLQSREQTEALQQ
                     LLRWSRYRDEVIELIESTRARLGEIYAAAVDDTDKRKLKEETFAEARRSHQAIAAKHG
                     ITGGFTAWFADKLNNAKIGSVSAYNSHRPAFVNMIRAHGLDFGAFYEYAETVGKLAKA
                     ERDACLAAWSRQQTADSECPAIKPGLATAS"
     gene            1211..2341
                     /locus_tag="VIW27_00185"
     CDS             1211..2341
                     /locus_tag="VIW27_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEY5738103.1"
                     /translation="MIISSQFDGGNIECVDSSDPGRVLLNIRNDNQSDFYQWFYFRAS
                     NVRDLDCRYLVGNAAGAAYQKGWQDYRAVASYDREFWFRVDTSFDGENLVIEHNSCED
                     QVYFAYFAPYSMERHADLLAFAQTSERCQAETLGLTLDGQSMDCLRFGEQDAGAKIWI
                     VARQHPGETMAEWWMEGLINRLVDHSDPVVNAILDKAVIYLVPNMNPDGSRRGHLRTN
                     AVGSNLNREWDNPTAEKSPEVLCVLDKISQTGLDFGLDVHGDEALPYNFIAGTEGIPG
                     WNRERQQQLELFKMTLANLNPDFQVEHGYPPNSAGNANMSYCSNSLAQRFGALVMTLE
                     MPFKDASGTSDAVQGWSPERCGRLAHSCLDALHLCWDQVINR"
BASE COUNT          506 a          732 c          750 g          488 t
ORIGIN      
        1 gtacgcgaat ttcatcaaga agcagtagca agcccgggtt tgattgccgg gcactcgctg
       61 tctgcggtct gctgccggct ccaggccgct aagcaggcgt cgcgctcggc tttagcgagc
      121 ttgccgacgg tttctgcgta ctcgtagaac gcaccgaagt cgagcccgtg cgcgcgaatc
      181 atgttgacga aggcaggcct gtgcgagtta taggctgaaa ccgagccgat cttggcgttg
      241 ttcagtttgt cggcgaacca ggccgtgaag ccacccgtta ttccgtgctt cgcggctatc
      301 gcctggtgcg agcggcgcgc ctcggcaaag gtttcttcct ttagtttgcg cttgtccgta
      361 tcgtcgaccg cagcggcata aatctcgccc agacgcgcgc gcgtggattc gatcagttcg
      421 atgacttcgt cgcggtaccg cgaccagcgc agcagttgct gcaatgcttc cgtttgttca
      481 cgcgactgca gccacagctc ggtgccgacc tgctgcaccg ccgaggcgag ggattcgttg
      541 aaggtcgtat cgtcttcgat atagatctgc tggtgagtaa gttcgtgaaa cagcaggccc
      601 gcgagccggt aatccggcca gttgatgaag ctgctcagca ccgggtcgtc gaaccagccc
      661 agcgtcgagt aagccgatac cctgccgacg tgtacctcga gacccgcggt ttcgagttcc
      721 tcgacgtagg cgagcagtcg cgtctcgtcg tagtaaccgc gatagctggc gcagccgatt
      781 atcgggtaac accactgccg caggcgggtc gaaaactcgg gcgcggcgaa caggttctgg
      841 accaggtagg gccggtcgag ttcgacatag ctcagataac tgtcgttgtc gggcagatcc
      901 agccgtgtca ccgagaactc gcgaatctcc cgcaccaggg tcaggcgttc gcgcagttcc
      961 ggcgtggtcg actgctccgc gagcaggtcc tcgatagcga cgcgcttatc catcacctcc
     1021 aggtgcccgc gcgcgctgtg ccagtagtaa cccatatcgg cacaaccacc gagcaataac
     1081 agcaaaggaa tcgaaattag gtaacgcata cagtattctc tcacactgac cagccggttt
     1141 ttttacccag cgcaaatttt accccgtggg ggaaatttta ctctggataa aatttacaca
     1201 ggaaaacagt atgatcatct ccagccagtt cgacggcggc aatatcgaat gcgtcgatag
     1261 cagcgacccg ggacgggtgc tgctcaatat ccgtaacgac aaccagtcgg atttttacca
     1321 gtggttctac ttccgcgcgt caaatgtgag ggacctggat tgccgctacc tggtcggcaa
     1381 cgccgcgggc gcggcctacc agaaaggctg gcaggattac cgtgcggtgg ccagctatga
     1441 tcgggaattc tggtttcgcg tcgataccag tttcgatggc gaaaacctgg tgatcgagca
     1501 caacagctgc gaggaccagg tttatttcgc ctatttcgca ccctattcga tggagcgtca
     1561 cgccgacctg ctcgccttcg cccagaccag tgagcgctgc caggccgaaa ccctgggcct
     1621 gaccctggat ggccagagca tggactgcct gcgtttcggc gagcaagacg cgggcgccaa
     1681 gatctggatc gtggcgcgcc agcatcccgg cgaaaccatg gcggaatggt ggatggaagg
     1741 cctgatcaac cggctggtcg atcacagcga tccggtggtc aacgcgattc tcgacaaggc
     1801 ggttatttac ctggtgccga atatgaatcc ggacggtagt cggcgcgggc acctgcgcac
     1861 caacgcggtg ggctcgaacc tgaaccgtga atgggataac ccgacggccg agaaaagccc
     1921 cgaggtgctg tgcgtgctcg ataaaatcag ccagaccggg ctggatttcg gcctcgacgt
     1981 gcatggcgac gaggcgctgc cgtataattt tattgccgga accgagggta tcccgggttg
     2041 gaaccgcgaa cgtcaacagc agctcgagct gttcaagatg acgctggcga atctgaatcc
     2101 ggatttccag gtggaacacg gctatccacc caatagcgcc ggtaatgcca acatgagcta
     2161 ttgttcgaat tcgctggcgc agcgcttcgg cgcgctggtg atgacgctgg aaatgccgtt
     2221 caaggatgcc agcggcacca gcgacgcggt gcagggctgg agcccggaac gctgcggacg
     2281 cctggcgcac tcctgcctcg acgccctgca cctgtgctgg gaccaggtga ttaatcgtta
     2341 accgagccgg ataagcccca tgcccaatcg aagagacttc ctcaaagcca gcgccgccgg
     2401 cctgctcggc gcgagcctgc tgcacccgat caggggccag gcaagtgttg cgcccttcaa
     2461 gctaaccgcg tccgag
//
LOCUS       DATCZV010000009         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_423662, whole genome shotgun sequence.
ACCESSION   DATCZV010000009 DATCZV010000000
VERSION     DATCZV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423662"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VIW27_00190"
     CDS             <1..869
                     /locus_tag="VIW27_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5738104.1"
                     /translation="ADCDLVAVVDRDAGLAREIAARHGAEAFHDHREILAGVDAISLV
                     VPTSLHYKIGREFLEAGIHCLIEKPITETVDEAETLIKIARDNDLVLQVGHIERFNSV
                     MQGIREHSHEPQFIESTRLAPFTLRATDVSVILDLMIHDIDLIVDLVDSPISRISASG
                     ISVLSNTIDIANARLEFENHCVANVTASRISRKRERKLRIFQKDAYLSADLQDKLLAV
                     NRKGETDNPDGFKDISHEEIRFADNDALNLEVLDFVNAIITGGRPQVDGDDGRRALQT
                     AIAITTQIRDAL"
     gene            866..1954
                     /locus_tag="VIW27_00195"
     CDS             866..1954
                     /locus_tag="VIW27_00195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5738105.1"
                     /translation="MTIPMVDLKGQYSSLKSEIDNAILEALGETRFILGPNVQEFDRE
                     AAAYLEVGHAISCASGTDALHLALLAAGIEAGDEVITSAFTFIATAEAIRYIGATPVF
                     VDVQPDSFNIDPQLIREAISERTRAILPVHLFGQPANMDEIITLAEEFELLLIEDCAQ
                     SFGSRYRGRVTGTIGAAGAFSFFPSKNLGCYGDGGMVTTNDDEIASRIRMLRNHGSSQ
                     QYHHDVIGFNSRLDELQAVILRIKLKHIDDYNRRRLQVAQAYNERLDGGRFQLPGIPT
                     DRDHVFHQYTLLCDGRDAVREALLAQDIACAIYYPIPLHRQQAFAATEQPDLPVAEET
                     ARRCLSLPIFPEMTLEQVDAVCEALANA"
     gene            1954..>2231
                     /locus_tag="VIW27_00200"
     CDS             1954..>2231
                     /locus_tag="VIW27_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY5738106.1"
                     /translation="MAAAHVMILAGEASGDAHAAEFAGILKAEHPEIAISGMGGSAMV
                     RAGVEVFFDSSSIAVVGLVEVARHWGDIKRAMTIVRQRLEQTRPDLLVL"
BASE COUNT          454 a          721 c          661 g          395 t
ORIGIN      
        1 gtgccgattg cgacctggtc gccgtggtcg accgcgatgc cgggctggcc cgggaaatcg
       61 ccgccaggca cggcgccgaa gcctttcacg atcaccgcga gatcctcgcc ggcgtcgatg
      121 cgatcagcct ggtagtgccg accagcctgc attataaaat cggccgcgag tttctggaag
      181 ccggaatcca ctgtttaatc gaaaaaccga tcaccgagac cgtcgacgag gctgaaaccc
      241 tgataaaaat cgcccgcgac aatgatctcg tgctgcaggt cgggcatatc gagcgcttca
      301 acagcgtcat gcagggaatt cgcgagcact cgcacgaacc gcagttcatc gagtcgacgc
      361 ggctggcgcc gttcacactg cgcgccaccg acgtcagcgt gatcctcgac ctgatgattc
      421 atgatatcga cctgattgtc gacctcgtcg acagcccgat cagccgcatc tcggcgagcg
      481 gtatctcggt attgtcgaat accatcgata tcgccaatgc acggctggag ttcgagaacc
      541 attgcgtggc caatgtcacc gccagccgta tcagccgcaa gcgcgagcga aaactgcgca
      601 tctttcagaa ggatgcctac ctgtcggccg acctgcagga caagctgctg gccgtcaacc
      661 gcaagggcga aaccgacaat cccgacggct tcaaggacat atcccacgag gaaatccgct
      721 tcgccgacaa cgacgccctc aacctggaag tgctggattt cgtcaatgcg atcatcaccg
      781 gcggccggcc gcaggtcgac ggcgacgacg gccggcgcgc gctgcaaacc gcgattgcaa
      841 ttaccaccca gatcagagat gccctatgac aatcccgatg gtcgacctca agggtcagta
      901 ttcgagcctc aaaagcgaaa tcgataacgc catcctcgag gccctcggtg aaacccgttt
      961 catcctcggc cccaacgtgc aggaattcga ccgcgaggca gccgcctatc tcgaggtcgg
     1021 tcacgcgatc agctgcgcgt cgggtaccga cgccctgcat ctggcgctgc tggcggccgg
     1081 catcgaggcc ggtgacgaag tcatcaccag cgctttcacc ttcatcgcca ccgccgaggc
     1141 gatccgctac atcggcgcga ccccggtatt cgtcgacgtt cagcccgaca gcttcaatat
     1201 cgatccacag ctgattcgcg aagcgatcag cgagcgcacg cgggcgattt tgccggtcca
     1261 tctgttcggg cagcccgcga acatggacga aatcatcaca ctggccgaag aattcgagct
     1321 gttgctgatc gaggattgcg cgcagtcctt cggtagccgt taccgcgggc gggtcaccgg
     1381 cactatcggc gcggccggcg cgttcagttt ctttccgagc aagaatctcg gttgttacgg
     1441 cgacggcggc atggtgacca ccaacgacga tgaaatcgcg tctcgaattc gaatgctgcg
     1501 caaccacggc agcagccagc aataccatca cgacgtaatc ggcttcaaca gccgactcga
     1561 cgagctgcag gcggtcattc tgcgcattaa gctgaagcat atcgacgact acaatcgccg
     1621 ccgcctgcag gtcgcgcagg cttacaacga acgcctcgac ggcgggcgtt ttcagctgcc
     1681 ggggatcccg accgaccgcg accacgtgtt tcaccagtac acgctattgt gcgacggccg
     1741 tgacgcggtg cgcgaggcgc tgctggcgca ggatatcgcc tgcgcgatct actacccgat
     1801 accgctgcat cgtcaacagg cgttcgccgc aaccgagcag ccggatttgc cggtcgccga
     1861 ggaaaccgcc cgccgctgcc tgtcattgcc gattttcccg gagatgacgc tcgagcaggt
     1921 ggatgccgtt tgcgaggcgc tggcaaacgc ctgatggccg ccgcgcacgt gatgatcctc
     1981 gccggcgagg cgtccggcga tgcgcatgcg gcggaatttg ccggcatctt gaaagccgag
     2041 catcccgaaa tcgcgatcag cggcatgggc ggcagcgcga tggtgcgcgc cggcgtcgag
     2101 gttttcttcg attcgtcgag tatcgccgtg gtcggcctgg tcgaagtcgc gcgccactgg
     2161 ggagatatca agcgcgcgat gacgatcgtc agacagcgcc tggagcaaac ccgccccgac
     2221 ctgctggtgc t
//
LOCUS       DATCZV010000010         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_741179, whole genome shotgun sequence.
ACCESSION   DATCZV010000010 DATCZV010000000
VERSION     DATCZV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741179"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1187)
                     /gene="ispE"
                     /locus_tag="VIW27_00205"
     CDS             complement(333..1187)
                     /gene="ispE"
                     /locus_tag="VIW27_00205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY5738107.1"
                     /translation="MVEALRVDSPAKLNLMLHITGRRADGYHLLETVFQFIDLCDRLE
                     FELDSGGRVTRHAPASPVAEEDDLLIRAAQLLRARCGERRGVRIRVDKRIPVGGGLGG
                     GSSNAASCLLVLNRLWGLGLTLDELAAIGLELGADVPVFVHGRAAWASGVGERLQPLD
                     LPCPVYLVIDPQTPVSTARIFAAEELTRNCDPLTIRAFLRGAGANVCEAVVRNRYPQV
                     AAALDWLGRHGEARMSGTGGCVFAAFDSLEQAERVKSQVPGEWAAFVARAMNRNPVHG
                     QLGLPITD"
     gene            complement(1189..1956)
                     /gene="lolB"
                     /locus_tag="VIW27_00210"
     CDS             complement(1189..1956)
                     /gene="lolB"
                     /locus_tag="VIW27_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY5738108.1"
                     /translation="MGQRRSRGSARGVAKGAAEISRRSHAAKSHAAVHTLKPAIRTPA
                     LCVALLALLTIAGCARQGSLDAGESAQQRWSAHRRALADYQTWDLHARAVVWRASEVY
                     NVGLQWRQDTRQFVMLLEAPFGQGVFRIDNGTDGGYRLLLPDGQVFKNRTPEALLEEV
                     FGWSLPISGLRYWIRGLPRPGSEYRDRLDNTGLARSLDQDRWSIEFREYFDAGREPQL
                     PRRLNLARDDLSIKLVIERWQRAEVEQQDAELFPEFD"
     gene            complement(1848..3584)
                     /locus_tag="VIW27_00215"
     CDS             complement(1848..3584)
                     /locus_tag="VIW27_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5738109.1"
                     /translation="MKVVSLPHRLASLAAVLLLAGCAGLQAPSFGDGELIGRQQAVVS
                     PNTSPDNAFADDAYALLVAEIALNQGDTALAVQQYLALARSQDSAEIAERAVRIAVYG
                     EDLEAAIEAAQRWIELDPQRIEARQVIAAIYVRQNRPEQAFDYLADMIRTSELDDQQL
                     FPPLLGILAREKNTDAVLAVSQRLAVEFPERAYAQYLHGMLAAQNGRAEEGLMYLDRA
                     AAIEEIEGVHSARAKILLRLGRPGEAVVSLRKAVESNPGDQNLRLTFARLLVDVKQYE
                     EARVEFEKLHRASPQDPELLYTLGLLSLESQRLDDAEKYMMMLVRLNEREGEAQYYLG
                     RIYENRKLYEDAIEWYSRVHVGEYSFDARLRIAGMLGTLGRTDEAVEHLDAMLKGSQS
                     NGSLVRIYIAKGELLRGARRYQQAMDIYNTALEIVPGNSDLLYARALVAEKLGRIDQL
                     EADIQTILKTEPDNAHALNALGFTLADQTDRYEEAYGYLERAIEIMPDDPAIIDSWGW
                     IHYRLGKYEEAIRLLRKALSRLDDAEISAHLGEVLWVSGDREEARAVWQRALQKSPDD
                     PMLQKVMQRFIP"
BASE COUNT          652 a         1269 c         1134 g          712 t
ORIGIN      
        1 cagggccgcc ctgcgggcgt ccaaattcgc tcccggcgaa tttgtcgaac caacgaatgc
       61 ccggatcaaa acctggtgcc ttaccacttg gcgacggccc aataaactat tacgccggcc
      121 cttcccggcc gcgcgatttt ccggctggca cgcaatttgc caaatccgcc ggctggattg
      181 cgaaatacct ccctgtattt cgcccttgcg gacgacctcc ggtcgtcctt cagggccgcc
      241 ctgcgggcgt ccaaattcgc tcccggcgaa tttgtcgaac caacgaatgc caggatcaaa
      301 acctggtgcc ttaccacttg gcgacggccc aattaatctg ttatcggcaa tcccagttgc
      361 ccgtgtaccg ggttacggtt cattgccctg gcaacaaatg ccgcccactc acccggaacc
      421 tgcgatttta cccgttccgc ctgctcaagg ctatcgaagg ccgcaaatac gcagccaccg
      481 gtcccgctca tccgtgcttc gccgtgccgc ccgagccagt cgagcgctgc ggccacctgt
      541 gggtagcgat tccgcaccac cgcttcgcag acgttagcgc ccgcgccgcg cagaaaggcg
      601 cgtattgtga gcggatcgca gttgcgtgtc aattcttccg cggcaaatat ccgcgccgtc
      661 gaaaccgggg tttgcgggtc gataaccagg tatacggggc aaggcaggtc gagcggctgc
      721 aagcgctcgc cgacaccgct cgcccaggcc gcccgaccgt gcacgaatac cggcacgtcg
      781 gcgccgagtt cgaggccgat ggcggccagt tcgtcgagcg tgagcccgag cccccagagc
      841 cggttcaata ccagcaggca ggacgccgca ttcgagctgc cgccgccgag gccgccgccg
      901 acgggaatcc gcttgtcgac gcggatgcgc actccgcggc gctcgccgca gcgcgcgcgc
      961 agcagttgcg cggccctgat cagcagatcg tcttcctccg ccaccggcga ggccggcgcg
     1021 tgacgcgtca cccggccgcc cgagtcgagt tcgaattcga gccggtcgca cagatcgatg
     1081 aactggaaca cggtctccag caggtggtag ccgtcggctc ggcgcccggt gatgtgcagc
     1141 atcaggttga gcttggccgg ggaatcgact cgcagcgcct cgaccatctc agtcgaactc
     1201 gggaaacaac tcggcgtcct gctgctcgac ctcggctcgc tgccagcgct cgatcaccag
     1261 cttgatgctg aggtcgtctc gcgccaggtt caggcggcgc ggcaactgcg gttcgcggcc
     1321 agcgtcgaaa tactcgcgga actcgatcga ccagcggtcc tggtcgagcg agcgggccaa
     1381 cccggtattg tcgagccgat cgcgatactc gctgccgggc cgcggcaggc cgcgaatcca
     1441 gtagcgcagg ccactgatcg gcagcgacca gccgaacact tcttcgagca aggcttcggg
     1501 cgtgcggttt ttgaacacct ggccgtccgg cagcaacagg cgatagccgc catcggtgcc
     1561 gttatcgatg cgaaacacgc cctggccgaa cggcgcctcg agcagcatca cgaattgccg
     1621 tgtatcctgc ctccattgca ggccgacatt gtagacttcg ctggcgcgcc agaccacggc
     1681 gcgcgcgtgc aggtcccagg tctggtagtc cgccagcgcc cggcggtgcg ccgaccagcg
     1741 ctgctgggca ctctccccgg catccaggct gccctggcgc gcgcagccgg cgatcgtcaa
     1801 cagcgccagc agggcgacgc agagcgccgg cgtccggatt gccggcttca gggtatgaac
     1861 cgctgcatga ctttttgcag catgggatcg tcgggagatt tctgcagcgc cctttgccac
     1921 accgcgcgcg cttcctcgcg atcgccgctg acccacagca cttcgcccag gtgcgccgag
     1981 atttcggcat catcgagtcg cgacagcgcc ttgcgcagca gccgaatcgc ctcctcgtac
     2041 ttgccgagac ggtagtgaat ccagccccaa ctatcgatga tcgcggggtc gtcgggcatg
     2101 atctcgatag cgcgctccag gtagccatag gcttcctcgt agcgatcggt ctgatcggcc
     2161 agcgtgaaac cgagcgcgtt gagcgcatgc gcgttatcgg gctcggtttt cagtatggtc
     2221 tgaatgtccg cctcgagctg atcgatacga cccagctttt cggcaaccag tgcccgcgcg
     2281 tagagcagat cgctgttgcc ggggacgatc tcgagtgcgg tattgtaaat atccatcgcc
     2341 tgctggtaac gccgcgcacc gcgcagcagc tcgcccttgg cgatgtatat gcgcaccagc
     2401 gaaccattcg actggctgcc cttgagcatc gcgtcgaggt gctcgacggc ttcgtcggtg
     2461 cgcccgagcg tgccgagcat gccggcgatt cgcagcctgg cgtcgaagct gtattcgccg
     2521 acgtgcaccc tgctgtacca ctcgatggca tcctcgtaca gcttgcgatt ttcgtaaatg
     2581 cgcccaaggt agtactgcgc ctcgccctcg cgctcgttga gccgcaccag catcatcatg
     2641 tacttctcgg catcgtcgag ccgttgtgac tcgagcgata gcaggcccag cgtgtacagt
     2701 aactcggggt cttgcggaga cgcccggtgc agtttttcga actccacgcg cgcctcttcg
     2761 tactgcttga cgtccaccag cagacgcgca aaggtcaggc gcaggttctg gtcgccagga
     2821 ttgctttcca ccgccttgcg caggctgacc acggcctcgc ccggccggcc caggcgcaac
     2881 aagatcttgg cgcgtgccga atgcacgccc tcgatctcct cgatcgccgc cgcacggtcg
     2941 agatacatca ggccttcctc cgcgcggccg ttttgcgccg ccagcatgcc gtgcagatac
     3001 tgggcgtagg cgcgctccgg gaactcaacg gcaaggcgct gcgataccgc gagtaccgca
     3061 tcggtatttt tttcgcgcgc caggataccc agcagcggcg gaaacaattg ctgatcgtcg
     3121 agttcgctgg tccttatcat gtcggcgagg taatcgaatg cttgctcggg ccggttctgg
     3181 cgcacgtaga tcgcggcgat gacctggcgc gcctcgatgc gctgcggatc gagttcgatc
     3241 cagcgttgcg cggcctcgat cgccgcctcg agatcttccc catagacggc gatgcgcacc
     3301 gcccgctcgg cgatttcggc gctgtcctgc gaacgcgcca gcgccaggta ctgctgcacc
     3361 gcaagcgccg tatcgccctg gttgagcgcg atttcggcca ccagcagcgc gtaggcatcg
     3421 tcggcgaaag cgttatcggg cgaggtgttc gggctgacca cggcctgctg cctgccgatc
     3481 agctcgccat cgccgaaaga cggcgcttgc aggccggcgc acccggccaa caacaggacc
     3541 gcagccaacg aggccaggcg gtgtggaagg ctgacaactt tcaagccggt ctccattcag
     3601 gtaaatccta tgattttagc acttagatgg ccgattgtat tcagtcccaa ttgctcgcat
     3661 attgttgtat ttggcgggtt tatacccgaa aatctgctgc tggcaacaat cctttcatct
     3721 atctatggct ttactgtccc tcggcatcaa tcacctgacc gctccgg
//
LOCUS       DATCZV010000011         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_318118, whole genome shotgun sequence.
ACCESSION   DATCZV010000011 DATCZV010000000
VERSION     DATCZV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318118"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VIW27_00220"
     CDS             <1..198
                     /locus_tag="VIW27_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEY5738110.1"
                     /translation="VGARNLLRTTEPVYKDADLDDDSLSDDEIIEAICEYPELLQRPI
                     VIAGDRAVIGRPPSRVLDLIA"
     gene            281..871
                     /gene="wrbA"
                     /locus_tag="VIW27_00225"
     CDS             281..871
                     /gene="wrbA"
                     /locus_tag="VIW27_00225"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY5738111.1"
                     /translation="MQARILIVYFSRHGGVARMAEQVAIGVDSNANCEAVLRCLPAVS
                     AASEQSEAPVPEQGVPYATVADLEDCAGLILGSPGYFGNMAAPLKYFLDQTSAQWLSG
                     ALIGKPAAVFTATSSLHGGQESVLLSMMLPLLHHGMLITGIPYSEPVLNQTRSGGTPY
                     GASHFAGADGRPLSEHEQDACRALGRRVAEIAGKLA"
     gene            885..1220
                     /locus_tag="VIW27_00230"
     CDS             885..1220
                     /locus_tag="VIW27_00230"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY5738112.1"
                     /translation="MAALKVVTLVSWSALLVCQLSLLLPSLAVSYYWSLPLIAALLIP
                     LRGLLSDRLYTYRWIGFMALVYLCVGISELAVNPALRAYGFGTTAASILLFLAVIYRA
                     RQLSAAAGR"
     gene            complement(1177..1710)
                     /locus_tag="VIW27_00235"
     CDS             complement(1177..1710)
                     /locus_tag="VIW27_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY5738113.1"
                     /translation="MQLYDAAQLLCCDAAAFDGYGDCDVLAVDNLDAVAGDAGWEAFF
                     YQAINRCRDGEYRFVYTLGARPENLPFGLADLRSRLQWGLLLQLPASGDAEVRDILRR
                     RAALLGFDLGSDVITYLMNRYPRDLSAQMSILRYLDGASLSQQRRVTIPLVRQALRER
                     REPTSGPPPQTVGEPDK"
     gene            1687..1953
                     /locus_tag="VIW27_00240"
     CDS             1687..1953
                     /locus_tag="VIW27_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738114.1"
                     /translation="MRGIVKLQIDAAFAGEVSTGIEQVRLAAGGAAPQADLRLAIATQ
                     QRLETADDQLVVRANEISEAELAIERQRKRDLFNCPPRGRYSRN"
     gene            complement(1916..3016)
                     /locus_tag="VIW27_00245"
     CDS             complement(1916..3016)
                     /locus_tag="VIW27_00245"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEY5738115.1"
                     /translation="MNLSKLLRLLPALMVACYSSAGAVIVEDLYTVELPIADQTTSLR
                     LDSFNEAFKQVLVKISGSDDPLKDPAFARPIERSARYVKQFRYVNRSQPGDDEFDASR
                     LHLRIDFDQQLVEGLLREHNYPIWGRERPSTLIVFSYDVNENIQLASQDTTPELIAAL
                     DEAASSHAVPVLFPLMDLEDMALVKIRDIAARQYEGIDTMAQRYSPNALLVGRIVGRT
                     GKGWEGDWEVRFAEQVFRWKHETSSRQDVIDQVIRQLAKILAREYALDDHSSVEQSLL
                     LAVSNVNGIGNLIRVQRYLESLNAVVGVRVAMINQDVVTYRLSLRNDPEDLQRLIEFG
                     EVLELEDFPRLSISGEDQIILNYGYIDRGVGN"
     gene            3100..>3620
                     /locus_tag="VIW27_00250"
     CDS             3100..>3620
                     /locus_tag="VIW27_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY5738116.1"
                     /translation="MNPEGSLTYRDSGVDIDAGNALVERIKPAIKATFREGCVGSIGG
                     FGGLFELPLSRYREPLLVSGTDGVGTKLKLAAELDALDGIGIDLVAMCANDIAVLGAE
                     ALFFLDYYASGRLQVEQAQRVIESIARGCSLAGCALIGGETAEMPGVYQGSDFDLAGF
                     CVGIVDKQQVIDG"
BASE COUNT          679 a         1184 c         1081 g          676 t
ORIGIN      
        1 gttggtgcgc ggaatctgct gcgcaccacg gaaccagtct acaaggacgc cgatctcgac
       61 gacgactcgc tctccgacga cgaaatcatc gaagccatct gcgaataccc cgaattgctg
      121 cagcgaccca tcgtcattgc cggcgacagg gcagtgatcg gccgcccgcc gagcagggtg
      181 ctggatctca ttgcttagcg cccatactcc agcgaatcgt taccgtaccc gtccgcggcc
      241 gtgcctcgcc gagtttcccg gcggcgaata tcggtgaacc atgcaggcta gaattctgat
      301 cgtctacttc agccgtcacg gcggtgtcgc ccgcatggcc gagcaggtcg cgatcggcgt
      361 cgactcgaat gccaattgcg aagccgtgct gcgctgcctg ccggcggtct ccgccgccag
      421 cgaacaaagc gaagccccgg tacccgaaca gggtgtgccc tatgcgacgg tcgccgacct
      481 cgaggattgc gccggcctga ttctcggcag ccccggttac ttcggcaata tggcggcgcc
      541 gctcaaatac tttctcgacc agaccagcgc gcagtggctg tccggcgcgt tgatcggtaa
      601 acccgccgcg gtatttaccg cgacctccag cttgcacggc gggcaggaat cggtactcct
      661 cagcatgatg ctgccgctgc tgcatcacgg catgctgatc accggcattc cctactcgga
      721 accggtactg aaccagaccc gcagcggcgg cacgccgtac ggcgcgagcc atttcgccgg
      781 cgccgacggc cggccgctca gcgaacacga acaggacgcg tgccgcgcgc tcggccgacg
      841 cgtcgccgaa atcgccggca agctggcctg agcggcatcg cgccatggcc gcgttgaaag
      901 tcgtcaccct ggtatcctgg tcggccctgc tggtctgcca gctgagcctg ttgctgcctt
      961 cgctggcggt cagctactac tggagcctgc cgttgatcgc cgcgttactg atcccgctca
     1021 ggggattgct cagcgatcgg ctgtacacct accgctggat cggtttcatg gcgctcgtat
     1081 acctgtgcgt cggcatcagc gaactggccg tcaacccggc actgcgcgcc tacggtttcg
     1141 gcaccacggc tgccagcata ctgctgtttc tcgcggttat ttatcgggct cgccaactgt
     1201 ctgcggcggc gggccgctag tcggttcgcg gcgttcgcgc agcgcctgcc tgaccagcgg
     1261 tatggtcacg cggcgctgct gcgacaggga tgcgccatcc aggtatctga ggatcgacat
     1321 ctgtgccgac agatcgcgcg ggtaacgatt catcaggtaa gtaatcacat cgctaccgag
     1381 gtcgaacccc agtaacgcgg cacgccggcg cagaatatcg cgcacctcgg catcaccact
     1441 ggccggcaac tgcaacagca gcccccactg cagccgggat cgcaggtcgg caagaccgaa
     1501 cggcaggttc tccgggcgcg cgccgagcgt ataaacgaaa cggtactcgc cgtcgcggca
     1561 gcgattgatc gcctggtaaa aaaaggcttc ccagccggca tcgccggcca ccgcatcgag
     1621 attatcgacc gccagcacgt cgcaatcgcc gtaaccgtcg aaagcggcag cgtcgcagca
     1681 tagcagttgc gcggcatcgt aaagctgcaa atcgatgccg cgttcgcggg cgaagtgagc
     1741 actggcattg agcaggtgcg tcttgccgct ggcggcgccg ccccacaggc cgatctgcgt
     1801 ctcgccatcg ccacgcagca gcgcctcgag actgccgacg atcagctcgt cgtgcgggca
     1861 aatgaaatta gcgaagctga actggcgatc gagcgacagc ggaagcgcga tttgtttaat
     1921 tgcccacccc gcggtcgata tagccgtaat tgagaataat ctggtcttcg ccgctgatgc
     1981 tcagacgcgg aaaatcctcc agttccagta cctcgccgaa ctcgatcagg cgttgcagat
     2041 cctcggggtc gttgcgcagg ctgagccgat aggtaaccac gtcctggttg atcatcgcca
     2101 cgcgcacgcc gaccaccgcg ttcagagatt cgagatatct ttgcaccctg atcaggttgc
     2161 cgataccgtt gacattggat accgcgagca acagcgactg ctcgacactg ctgtgatcat
     2221 cgagcgcata ctcccgcgcc aggatctttg ccagctgccg aatcacctgg tcgatgacgt
     2281 cctgccgcga ggacgtctcg tgcttccagc gaaacacctg ctccgcaaac ctcacttccc
     2341 agtcaccctc ccagcccttg ccggtgcggc cgacgattct gcccaccagc agcgcgttcg
     2401 gcgaatagcg ctgcgccatg gtgtcgatcc cctcatactg gcgcgcggcg atatcgcgaa
     2461 tcttgaccag cgccatgtcc tcgagatcca ttagcggaaa cagcaccggt accgcgtggc
     2521 tcgacgccgc ctcgtcgagc gccgcgatca gttccggggt cgtatcctgc gacgccagct
     2581 ggatattttc gttgacgtcg taggagaaaa caatcagcgt actcggccgc tcgcgccccc
     2641 agatcgggta attgtgttcg cgcaacaggc cctcgaccag ttgctgatcg aaatcgatgc
     2701 gcaggtgcag acggctggca tcgaattcgt cgtcgccggg ctggctgcga ttgacatagc
     2761 ggaattgctt cacataacgg gcgctgcgct cgatcggacg cgcgaaggcc ggatccttca
     2821 acgggtcgtc ggagccgctg atcttgacca gtacctgctt gaaggcctcg ttgaaggaat
     2881 ccaggcgcag gctggtggtc tgatcggcga tcggtaactc gaccgtgtac aggtcttcga
     2941 cgatcaccgc tcccgccgac gagtagcagg caaccatcag tgccggcagc agccgcaaca
     3001 gcttcgacaa gttcatattt tattcttctc cgggcacgga catttgcaaa cgaggcgtgt
     3061 aaaatagcac acccctaata tttcctgaac cggcaaacca tgaatcccga gggatccctg
     3121 acctatcgcg attccggcgt cgatatcgat gccggcaacg cgctcgtcga gcgcatcaaa
     3181 ccggctatca aggcgacctt ccgcgagggc tgtgtcggca gtatcggcgg cttcggcggt
     3241 ctgttcgaac tgcccctgtc gcgctatcgc gaacccctgc tggtatcggg taccgacggc
     3301 gtcggcacca agctcaagct ggccgcggaa ctcgatgcgc tcgacggtat cggcatcgac
     3361 ctggtggcga tgtgcgccaa tgatatcgcg gtactcggtg ccgaggcgct gttctttctc
     3421 gattactacg ccagcggccg gctgcaggtc gaacaggcgc agcgcgtgat cgagagcatc
     3481 gcccgtggct gcagcctcgc cggctgcgcg ctgatcggcg gcgaaaccgc ggaaatgccc
     3541 ggggtttacc agggcagcga tttcgatctc gccggctttt gcgtcggcat cgtcgacaag
     3601 cagcaagtca tcgacggcag
//
LOCUS       DATCZV010000012         3897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_212408, whole genome shotgun sequence.
ACCESSION   DATCZV010000012 DATCZV010000000
VERSION     DATCZV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212408"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..1924
                     /gene="gspE"
                     /locus_tag="VIW27_00255"
     CDS             449..1924
                     /gene="gspE"
                     /locus_tag="VIW27_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY5738117.1"
                     /translation="MASTGDLPYSFCKRYGVIAGLSPVSGKLRLTLKDGAPADAVSEA
                     QRLLGMVDEIEVESDLEFNRLLSKHFENNRETSFIEAEKIGGELNLDDVAKELQEPED
                     LLESDDDAPIIRLINALITEAIKENASDIHVEPFEARLSIRFRVDGVLREVLEPPRQI
                     ASLLVSRIKVMAQLDIAEKRLPQDGRIALRVANRPVDIRVSTLPSGHGERVVMRLLDK
                     QAGRLDLMELGMGEVLEKRLESMIQKPHGIILVTGPTGSGKTTTLYAMLSVLNDTKRN
                     ILTVEDPIEYDLDGIGQTPVNTKVNMTFARGLRAILRQDPDVVMVGEIRDSETAQIAV
                     QASLTGHLVLSTLHTNSAVGAITRLEDMGVEPFLISSTLLGVISQRLVRKLCPECKSV
                     TQLDVEQINGFEIKGDDLVYQANGCPACLQTGFRGRTGIYEMIEVDANLSSMIHDSTS
                     QAKIEKYCRTMSRSLREDGIRKVLLGETTLDEVLRVTRDGG"
     gene            2240..3451
                     /gene="gspF"
                     /locus_tag="VIW27_00260"
     CDS             2240..3451
                     /gene="gspF"
                     /locus_tag="VIW27_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY5738118.1"
                     /translation="MAAYEYQAIDADGKTRKGLIEADTARQARQQLRGMSLMPLEIGE
                     VSSREAVGGKGRHKRDKLNVTTLSLITRQLATLIAAGQPIESAYHAVSRQTPKPAAKR
                     ILIAVRARVLEGYSLSDALREFPRVFDSMYCASVHAGEQSGLLDVVMERLADHLEARQ
                     ELQRRTGQALIYPILLTVTAFALVAALLTFVVPKIVQVFEGFDQELPLMTTWLIGISE
                     FFKSWGVELIVGVVLLMVVYAQLVKFPAFCLLRDRFYLRLPYVSFLVRLANTARFTRT
                     MSILVSSGVTALDAMQISTEVIGSQPIRMAVMKAASKVREGGLISVSLEQTGYFSPLV
                     VQLIQIGEASGKLGEMLERSARAEENEFAGVTALFIGLFEPAMILVMGLVVLFIVIAI
                     LIPIFDMNELV"
     gene            3448..>3897
                     /gene="rrtA"
                     /locus_tag="VIW27_00265"
     CDS             3448..>3897
                     /gene="rrtA"
                     /locus_tag="VIW27_00265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEY5738119.1"
                     /translation="MIEWQRIRHSGDWRFVGIFVAIAVVLQIIGPEYFRYQSDWPQSG
                     QLWRVVSAHWVHVGWMHLLLNTLGLAICVTLTTPGWSLQRWFVQSLLLAVGISLLCTL
                     QNPEISWYAGFSGVLFGLYFLCAHDLYRRDRLIAVLVASAIVIKVLLE"
BASE COUNT          716 a         1175 c         1280 g          726 t
ORIGIN      
        1 ttgagaagat tgcggcgcgg aggccgcaat gacggggcag gggcgtgact gccgcgccgc
       61 aatgacggct cagggtgcgt cgggaagcgg ccatagcacc gccgggaaga cgccaacccc
      121 gtcatgccgg gctcgacccg gcatctttcc cgaaccggga gcgattgtcg cagcgcgaca
      181 accgtggcga tcctgagcgg aggtgccggg tattgagaag attgcggcgt ggacgggcgg
      241 caggcggcgc cgcaatgacg gggccgggca cgccgcgttg cgagggttat tgactggctg
      301 ctgcagagtc ttctgcccga atgcgacggg cgccacagcg caaagatacg ttttattgac
      361 atgattcagc taactttcag ggtttttatg gtcaaatggg cgtcgaaacc ctactataat
      421 tcagtaacat agacggataa gttcccaaat ggcttcaaca ggcgatcttc cctattcatt
      481 ctgcaagcgc tatggcgtca tcgccggcct gtccccggtg tcgggcaagc ttcggctgac
      541 gctcaaggac ggcgcgccgg ccgacgcggt ttccgaggcg cagcgcctgc tcggcatggt
      601 cgacgagatc gaggtcgaat ccgacctcga gttcaaccgc ctgttgagca agcatttcga
      661 aaacaaccgc gagacctcgt tcatcgaggc cgagaagatc ggcggcgagc tcaatctcga
      721 cgacgtcgcc aaggagctgc aggagcccga ggacctgctc gaaagcgacg acgatgcgcc
      781 gatcattcgc ctgatcaacg cgctgatcac cgaggcgatc aaggagaatg cgtcggatat
      841 tcacgtcgaa ccgttcgagg cccggctgtc gattcgcttt cgcgtcgacg gcgtgctgcg
      901 cgaggtactc gagccgccgc ggcagatcgc cagcctgctg gtgtcgcgga tcaaggtcat
      961 ggcgcagctc gacatcgccg agaagcgcct gccgcaggac ggccgcatcg cgttgcgcgt
     1021 tgccaaccgg ccggtcgaca ttcgcgtctc gaccctgcct tcgggccatg gcgagcgcgt
     1081 ggtcatgcgc ctgctcgaca agcaggccgg gcggctcgac ctgatggagc tcggcatggg
     1141 cgaggtgctc gagaagcgcc tcgagagcat gatccagaag ccgcatggca ttatcctggt
     1201 caccggcccg accggctcgg gcaagaccac cacgctgtac gcgatgctgt cggtgctcaa
     1261 tgacacgaag cgcaatatcc tcaccgtcga ggacccgatc gagtacgatc tcgacggtat
     1321 cggccagaca ccggtcaata ccaaggtcaa catgaccttc gcccgcggcc tgcgcgcgat
     1381 cctgcgccag gacccggacg tggtcatggt cggcgagatt cgtgacagcg agaccgcgca
     1441 gattgcggtg caggcgagcc tcaccggtca cctggtgctg tcgacgctgc ataccaattc
     1501 ggcggtcggc gcgattacgc ggctcgagga tatgggtgtc gaacccttcc tgatttcgtc
     1561 gacgctgctc ggcgtgatct cgcagcgcct ggtgcgcaag ctgtgccccg aatgcaagtc
     1621 ggttacccag ctcgacgtcg aacagatcaa cggtttcgag atcaagggtg acgacctggt
     1681 ctaccaggcc aacggttgcc cggcctgcct gcagacgggt tttcgcgggc gtaccggcat
     1741 ctacgaaatg atcgaggtcg atgcgaacct gtcgtcgatg attcacgaca gcaccagcca
     1801 ggccaagatc gagaagtact gccgcaccat gagccgcagc ctgcgcgagg acggcattcg
     1861 caaggtgctg ctgggcgaaa ccacgctcga tgaagtgctg cgggtaaccc gcgacggggg
     1921 ctagcgatgc tagccgatgg caatagattc cgggtcaagc ccggaatgac gcaaggctcc
     1981 gtcattgcgg gccccgaccc gcaatccatt ttgtcgcgag gaagccctgg cgcattccgc
     2041 cggattggca catcgctcgg gcgcggaacg acgaaacccc tcaatcgcaa aagattccgg
     2101 gtcaagcccg gactgacgat agtggacggt tgcgatggat tccgggtcaa gcccggaatg
     2161 acgatagtgg acggttgcga tgaattccgg ctcgcaggcg gaatgacgac gatagcggga
     2221 ttcgagggct aggtcggcga tggcggctta cgaataccag gccatcgatg ccgatggcaa
     2281 gacccgcaag ggtttgatcg aggccgatac cgcgcgccag gcgcgccagc agctgcgcgg
     2341 catgagcctg atgccgctgg aaatcggcga ggtttcgtcg cgcgaggccg tcggtggcaa
     2401 ggggcggcac aagcgcgaca agctcaacgt caccacgctg tcactgatca cccgccagct
     2461 ggcgaccctg atcgccgccg ggcagccgat cgagtcggcc taccacgcgg tctcccggca
     2521 aacgccgaaa ccggccgcca agcgcatcct gatcgcggtg cgcgcgcgcg tgctggaagg
     2581 ctactcgctg tcggatgccc tcagggagtt cccgcgggtg ttcgattcca tgtactgcgc
     2641 atcggtgcat gccggcgagc aatcgggact gctcgacgtg gtcatggaac gactcgccga
     2701 ccacctcgag gcgcgccagg aactgcagcg gcgcaccggc caggcgctga tctacccgat
     2761 cctgctgacg gtgaccgcgt ttgcgctggt cgccgcgctg ttgaccttcg tggtaccgaa
     2821 aatcgtgcag gtattcgagg gtttcgacca ggaattgccg ttgatgacca cctggttgat
     2881 cggcatctcc gagttcttca agtcctgggg cgtcgaattg atcgtcggcg tggtattgct
     2941 gatggtggtg tacgcgcagc tggtcaagtt tcccgctttt tgcctgctgc gcgaccgctt
     3001 ttacctgcgc ctgccgtacg tctccttcct ggtgcggctg gcgaacaccg cgcgcttcac
     3061 gcgcaccatg agcatcctgg tatcgagcgg cgtgaccgcg ctcgacgcga tgcagatttc
     3121 caccgaggtc atcggcagcc agccgatccg catggcagtg atgaaagccg ccagcaaggt
     3181 gcgcgagggc gggctgatct cggtatcgct ggagcagacc ggttatttca gtccgctggt
     3241 ggtgcagctg attcagatcg gcgaggccag cggcaagctc ggcgaaatgc tggagcgctc
     3301 ggcgcgcgcc gaggaaaacg aattcgccgg cgtcaccgcg ctgttcatcg gcctgttcga
     3361 gccggcgatg atcctggtga tgggtctggt cgtattgttc atcgtgatcg cgatcctgat
     3421 cccgattttc gatatgaacg agctggtatg atcgagtggc agcgtattcg gcattccggc
     3481 gactggcgat tcgtgggcat attcgtggcg atcgcggtcg tgctgcagat catcggtccc
     3541 gaatatttcc gctaccagag cgactggccg caaagcggac agctgtggcg cgtggtcagc
     3601 gctcactggg tgcacgtcgg ctggatgcac ctgctgctga atacgctcgg gctggcgatc
     3661 tgcgtaaccc tgacgacacc gggctggagc ctgcaacgct ggttcgtgca aagcctgttg
     3721 ctggcggtcg gcatctcgct gctgtgcacg ctgcaaaacc cggagatttc gtggtacgcg
     3781 ggtttctcgg gcgtgctgtt cggcctctat tttctctgcg cccacgatct ttaccggcgc
     3841 gaccggctga tcgcggtgct ggtggcgagc gcgatcgtga tcaaggtcct gctcgag
//
LOCUS       DATCZV010000013         4039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_424142, whole genome shotgun sequence.
ACCESSION   DATCZV010000013 DATCZV010000000
VERSION     DATCZV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424142"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="murA"
                     /locus_tag="VIW27_00270"
     CDS             <1..1005
                     /gene="murA"
                     /locus_tag="VIW27_00270"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5738120.1"
                     /translation="YELVKTMRASILVLGPLLARYGKAEVSLPGGCAIGARPVDMHLS
                     GLHAMGADITVEQGYIKARCKRLKGARIVMDQVTVTGTENLMMAASLARGTTVIENAA
                     REPEVVDLADFINAMGGQVENAGTDKITIHGVDNLEGCDYSVLPDRIETGTYLVAAAI
                     TGGKVLIKHTDPNLLDAVLAKLEEAGATIDLGDDWIELDMEGRKPQAVNIRTAPYPGF
                     PTDMQAQFCALNSIAEGTGSVTETVFENRFMHIQEFMRLGANVQLEGNTVVIRGVDRL
                     NGAQVMATDLRASASLILAGLVARGETIVDRIYHIDRGYDHIEEKLAGLGAQIQRVPN
                     "
     gene            1011..1640
                     /gene="hisG"
                     /locus_tag="VIW27_00275"
     CDS             1011..1640
                     /gene="hisG"
                     /locus_tag="VIW27_00275"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY5738121.1"
                     /translation="MPNPLTIALAKGRILDETLPLLARAGIEPAEDLGSSRKLIVASN
                     LDKVRLVLIRSADVATYVQYGAADLGIAGKDVLAEHGGDGLYERLDLKIAPCRMMTAG
                     FADRPLPRGRLRVATKYPRITRGFFHDQGRQVELIKLYGSMELAPIVGLADVIVDLVD
                     TGNTLQANGLTPLEHIMDISSRLIINRASLKMKNRAIEALVSRIEQAVE"
     gene            1637..2956
                     /gene="hisD"
                     /locus_tag="VIW27_00280"
     CDS             1637..2956
                     /gene="hisD"
                     /locus_tag="VIW27_00280"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5738122.1"
                     /translation="MTIEILRLDRSDGDFWERLERLLAWEAVADDTVSATTREIVQAV
                     RRDGDRALLEYSRRFDSIDADTVAELEISPQRLRQAFDEIDAEDRAALQQAAARIRAY
                     AERQKLESWEYTEADGTLLGQQITPLDRAGLYVPGGKAAYPSSVLMNAIPAAVAGVGE
                     LIMVVPTPRGEVNPLVLAAAHLAGVQRVFGIGGAQAVAALAYGTETVPAVDKIVGPGN
                     IYVAAAKRLVYGQVGIDMLAGPSEILVVCDGATDPDWIAMDLFSQAEHDEDAQAILVS
                     DDAAFLDRVQQSIDRLIEELPRRAIVEQSLTSRGALIEVANLDEAVEVANYIAPEHLE
                     LSVANAVELAPSIRHAGAIFLGRYTAEALGDYCAGPNHVLPTSRSARFSSPLGVYDFQ
                     KRSSLIGCSAAGASALGRVASRLARGEGLVAHARSAENRIDSEDQAG"
     gene            3229..4026
                     /locus_tag="VIW27_00285"
     CDS             3229..4026
                     /locus_tag="VIW27_00285"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY5738123.1"
                     /translation="MQQTLRSTLRKILMSTIRILLRNGMSYGEFDQLARKCFVDVAFD
                     DFAPSGKKQTVSNVAILTGLNRKEVKKLHEFDADHGSAGARQYNRAVRVLGGWLNDSR
                     YLRKDGNPRDLEYEGEGSFSELVKQYSGDMPVAAMQKVLTQSGNIGVTDNNQVRLLSH
                     AYLPADDPAEKLAILGSDTSELIDTIDYNLTAPEEALRFQRKASNPRVAVASIPEIKQ
                     FLRRKGQAFLEEVDLYFTQHEVGDDEPGAKLSVSVFYHEAENNSKED"
BASE COUNT          781 a         1281 c         1285 g          692 t
ORIGIN      
        1 tatgaactgg tgaagaccat gcgcgcgtcg attctggtac tcggaccact gctggcgcgt
       61 tacggcaagg ccgaggtgtc gctgccgggc ggctgcgcga tcggcgcccg gccggtcgac
      121 atgcacttga gcggcctgca cgcgatgggc gccgacatca ccgtcgagca gggctatatc
      181 aaggccaggt gcaagcgcct caagggtgcg cgcatcgtca tggaccaggt caccgtgacc
      241 ggcaccgaga acctgatgat ggcggccagc ctcgcccgcg gcaccacggt gatcgaaaac
      301 gcggcgcgcg agcccgaggt ggtcgatctc gccgatttca tcaacgcgat gggtggccag
      361 gtcgaaaatg ccggcaccga caagatcacc attcacggtg tcgacaacct cgagggctgc
      421 gattactcgg tgctgccgga tcgtatcgaa accggcacct acctggttgc cgccgcgatc
      481 accggcggca aggtgctgat caagcacacc gatcccaacc tgctcgatgc cgtgctcgcc
      541 aagctcgagg aagcaggggc gacgatcgat ctcggcgatg actggatcga actcgatatg
      601 gaaggccgca agccgcaagc ggtcaatatc aggacggcgc cttaccccgg tttcccgacc
      661 gacatgcagg cgcagttttg cgcgctcaac tcgattgccg agggcaccgg ctcggtcacc
      721 gaaaccgtgt tcgaaaaccg cttcatgcat atccaggaat tcatgcggct aggcgccaat
      781 gtgcagctag aaggcaatac cgtggtgatt cgcggtgtcg accggctcaa cggcgcccag
      841 gtcatggcta ccgacctgcg cgcctcggcg agcctgattc tcgccggcct ggtggctcgc
      901 ggtgaaacta tcgtcgaccg catctaccac atcgatcgcg gctacgatca catcgaggaa
      961 aagctcgccg ggctcggcgc ccagatacaa cgcgttccga actgattccg atgccaaatc
     1021 cgttgaccat cgcgctcgcc aagggcagga ttctcgacga aaccctgccg ctgctggcgc
     1081 gcgccggcat cgaacccgcc gaggacctcg gcagcagccg caagctgatc gtcgccagca
     1141 acctcgacaa ggttcgcctg gtgctgatcc gttccgccga tgtcgccacc tacgtgcagt
     1201 acggcgccgc cgatctcggc atcgccggca aggacgtgct ggcggagcac ggcggcgacg
     1261 ggctttacga gcggctcgac ctgaaaatcg ctccctgtcg catgatgacc gccggctttg
     1321 ccgaccggcc gttgccgcgc ggccggttgc gcgtggctac caagtatccg cgcatcaccc
     1381 gcggtttttt tcacgaccag ggccgccagg tcgaactgat caagctctac ggctcgatgg
     1441 aactggcgcc gatcgtcggc ctcgccgacg tcatcgtcga cctggtcgat accggtaata
     1501 cgctgcaggc caacgggctg acgccgctgg agcatatcat ggacatcagt tcgcgcctga
     1561 tcatcaatcg cgcttcgctg aaaatgaaaa accgcgcaat cgaggcgctg gtctcgcgaa
     1621 tcgagcaggc cgtcgaatga cgatcgaaat tttacgcctc gaccgcagtg acggcgattt
     1681 ctgggagcgg ctcgagcggc tgctggcctg ggaagcggtt gccgacgaca cggtcagcgc
     1741 caccactcgc gagatcgtgc aggcagtccg aagagacggc gaccgcgcgt tgctcgagta
     1801 cagccgcagg ttcgattcga tcgacgccga cacggtggca gagctggaaa tctcgccgca
     1861 gcggctgcga caggcgttcg acgaaatcga tgccgaggac cgcgccgcgc tgcaacaggc
     1921 cgcggcgcgg attcgcgcct atgccgagcg tcagaaactc gaatcctggg aatacacgga
     1981 ggccgacggc accctgctcg ggcagcagat cacgccgctc gaccgcgccg ggctctacgt
     2041 gccgggtggc aaggccgcgt acccgtcatc ggtgctgatg aacgcgattc cggccgcggt
     2101 tgccggggtc ggcgaactga tcatggtggt gccgacgccg cgcggcgaag tcaacccgct
     2161 ggtgctggcg gccgcccacc tggccggtgt gcagcgcgtg ttcggcatcg gcggcgcgca
     2221 ggccgtggcc gcgctcgcct acggtaccga gaccgtgccc gcggtcgaca agatcgtcgg
     2281 tccgggcaat atctacgtgg ccgcggccaa gcggctggtc tacgggcagg taggtatcga
     2341 catgctcgcc gggccttcgg agatactggt ggtctgcgac ggtgccaccg atcccgactg
     2401 gatcgcgatg gacctgtttt cgcaggccga gcacgacgag gacgcgcagg cgatcctggt
     2461 ttccgatgac gcggcattcc tcgatcgcgt gcagcagagt atcgaccggt tgatcgagga
     2521 attgccacgc cgcgcaatcg tcgaacagtc gctgacttcg cgcggcgcgc tgatcgaggt
     2581 cgccaacctc gacgaagccg tcgaggttgc caattacatc gcccccgagc acctcgagct
     2641 gtcggtcgcc aacgccgtcg agctggcgcc gtcgatacgc catgccgggg cgatatttct
     2701 cggccgttac acggccgagg cgctcggcga ttattgcgcc gggcccaacc acgtgctgcc
     2761 gacctcgcgc agtgcacgct tctcgtcacc actcggtgtc tacgactttc agaaacgcag
     2821 cagcctgatc ggctgttcgg cggccggcgc ttcggcgctc ggcagggtgg cgtcgcggct
     2881 ggcgcgcggc gagggcctgg tggcgcacgc gcgctccgcc gaaaaccgca tcgacagcga
     2941 agaccaggcc ggctagcgcc gcccggtggc cggccgattg ccggccggcg aatccttgcc
     3001 ggggttcctg ccggcgagtt cattttgtga cctgggtaaa atttttgggc tacagggctt
     3061 gatttatatt gatatgggaa tattatccca aaatagattg agtaatcggg ccctggtagc
     3121 ccattatgac acgaggaata tcccggcagc cgaacggcgc ggggtgaccg gtgcggcaga
     3181 tgccgctgtg ccgagacagc ggcacccgga cctggcgact tgacaactat gcaacagacc
     3241 ttacgtagca cactccgcaa aatcctgatg tcgacaattc gcatcctgct gcgaaatggc
     3301 atgtcctacg gtgaattcga tcagctggcg cgcaagtgtt tcgtcgacgt cgcgttcgac
     3361 gacttcgcgc cctccggcaa gaagcagacg gtttccaacg ttgctattct caccggcctg
     3421 aatcgcaagg aagtcaagaa gttacacgaa tttgacgccg atcacggatc cgcgggcgcc
     3481 aggcaataca atcgagcggt cagggtgctt ggcggctggc tcaacgattc gcgctacctg
     3541 cgcaaggatg gcaatccgcg cgacctcgaa tacgagggcg aaggctcgtt cagcgagctg
     3601 gtaaagcagt acagcgggga catgccggtg gcggcgatgc aaaaggtgtt gacccagtcg
     3661 ggaaatattg gcgtcaccga caacaaccag gtgcgcctgt tgagccacgc ctacctgccg
     3721 gccgacgacc cggcggagaa gctcgcgata ctcggttccg ataccagcga gctcatcgat
     3781 accatcgatt acaacctgac ggcgcccgag gaggcgctgc gtttccagcg caaggcctcc
     3841 aacccgcggg tcgcggtagc gtcgatcccc gaaatcaagc aattcctgag gcgcaaggga
     3901 caggcttttc tcgaggaggt cgacctttat tttacccagc acgaggttgg cgacgacgaa
     3961 cccggcgcaa aactcagcgt ctcggtgttc taccacgagg ccgagaacaa ttcgaaggaa
     4021 gattgacagc catgaatcg
//
LOCUS       DATCZV010000014         4611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_783, whole genome shotgun sequence.
ACCESSION   DATCZV010000014 DATCZV010000000
VERSION     DATCZV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..2575
                     /locus_tag="VIW27_00290"
     CDS             365..2575
                     /locus_tag="VIW27_00290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5738124.1"
                     /translation="MNDHTILRIAINAPLSRLFDYLPPEGVAAADLRPGCRLRVPFGH
                     RQVVGMLLEVGADSPLPRKKLKRAGALLDAQPLLSERDLWLLRFTADYYHHPIGEVVA
                     AALPAMLRRGRHLDESLEIVTLTEEGLAANLVALRRRAPRQFEFMEILLAADNQALDT
                     ASMEQQLPGWRRQKAALLERGWIRIDYISQAEHGESVIEGEAGPVLNDDQQRAVAEIL
                     ATPGFGVTLLDGVTGSGKTEVYLRLMQSVLAAGKQVLILVPEIGLTPQFVGRLEQRLG
                     RKPALLHSALSDSARLAAWRAARSGAASLLIGTRSAVFTPLANPGLIIIDEEHDSSFK
                     QQEGLRYSARDLGIVRAKQMDIPVVLGSATPSLESLHNCEEGNFRRLELPSRAGAANP
                     PRLRLVDTSHLSASESLSPALTEAMRAHLDAGNQVLLFLNRRGFAPTLICVACGHIAD
                     CPRCDARMTVHAKSAQLLCHHCGNSRPLQQHCSECGAECRPLGHGTERLENVLLDKFP
                     GQSIARIDSDSVQRKGMMDQALELATSGEASILVGTQMLSKGHHFPQLTLVGVINADQ
                     GLFGTDFRAAEKLAQGLIQVAGRAGREDRAGEVLIQTSFPKHAFWQELLSGGYPRVAA
                     AALQERRLSGWPPYSRLALIRSSSHRREDARRFLERARSLAAGLQIAEVNLLGPVGAA
                     MERRAGRYRAQLLLQSASRAALHSLLRRLRPLLEEDKSVKRVRWSIDIDPIELA"
     gene            2677..4431
                     /gene="argS"
                     /locus_tag="VIW27_00295"
     CDS             2677..4431
                     /gene="argS"
                     /locus_tag="VIW27_00295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY5738125.1"
                     /translation="MKQSIAQIIESTVRSIPELAALPELETIAAVVERTRDSKHGDFA
                     TSIAMQLAKPARRNPRELAQLIVDHLPATPLIDKVEIAGPGFINFYIGAGAFHEELLH
                     VLDQGADYGTQTCRESPRYLLEFVSANPTGPLHVGHGRHASFGASVGSLMRAAGYAVD
                     LEYYVNDAGRQMDILGASVFLRLLQSRGEDVAIPAAGYRGDYIRDIAAQIKGVEVGDI
                     TAAALLQGLPADEAQGGDKEAYITAVIDRAQALLGTEAFASLRQQSLSLIRDEIREDL
                     LEFGVRFDNWYSEQSLEANDLIDKALAVLQERGMLYKQDGATWFRSSDFGDEKDRVVI
                     RENGKKTYFASDIAYHFDKRQRGYDHLIDVLGADHHGYVARVRAGLQAFGYAGDSFEV
                     PLIQFVSLYRSGEKVAMSTRSGDFVTLRQLREEVGNDAARFFFVMRSNDQHLDFDLEL
                     AKSRSNDNPVYYIQYAHARVASVFRQLAEKLLAWDREQARTGLALLTAEHEKALMATM
                     SRYPEVIDLAARNRAPQHLVHYLRDLANDFHTYYNAHVFLVEDAALRNARLLLIEATR
                     QVVANGLGILGVSAPESM"
BASE COUNT          858 a         1470 c         1452 g          831 t
ORIGIN      
        1 cggaacaccg attttcatgc gttacgcctc catttggatg gcggcagtct accgaagcac
       61 ccgagggact gctaggcatt ttagtaaggg gtttccgaaa catccgtcgc gttttctgga
      121 gcgcaaagcg gcctgaaatt gtccctgcag aagggctttg cgcgcccttg tggcgcacgg
      181 ccggcggcag gccatggcgg cccgccatta ccgaccgctg ttcggcccac cggcgcaccg
      241 gatcggtgga tcggaatctt ccctcgcgcc tcccgcggcg gctgactcgc cgtggcaatc
      301 gccgcccgca tcggtatcat ggatccacct gctgcggcag catcgtcgtt agactgaagg
      361 cggaatgaac gaccacacta tattaagaat cgcgataaac gccccgttat ctcgcctctt
      421 tgattacctg ccacctgagg gcgtcgccgc cgcggacctg cggcccggct gccggctgcg
      481 cgtgcccttt ggtcatcgcc aggttgtcgg catgttgctg gaagttggcg ccgacagccc
      541 cctgccgcgc aagaaactaa agcgtgccgg cgcactgctg gacgcgcagc cgttactgtc
      601 ggaacgcgat ttgtggctgc tcaggtttac cgccgattac taccaccacc cgattggcga
      661 agtcgtcgcc gcagcattgc cggccatgct gcggcgcggg cgccacctcg atgaatccct
      721 ggagatcgtg acgctgaccg aggaaggcct ggccgccaac ctggtagcac tgcgccgccg
      781 cgcaccgcgc cagttcgagt tcatggagat tctcctggcc gccgacaacc aggcgctgga
      841 cacggcctcg atggaacagc agctgccggg atggcggcgg caaaaggcgg cgctgctcga
      901 gcgcggctgg attcgaatcg attacatcag ccaggcggaa cacggcgaga gcgtcatcga
      961 gggggaggcc ggcccggtac tcaacgatga ccagcaacgc gcggtggccg agattctcgc
     1021 cacgcccggg ttcggagtga ccctgctcga cggcgtcacc ggaagcggca agaccgaggt
     1081 gtatctgcgg ctgatgcagt cggtcctggc ggccggcaag caggtgctca tcctggtgcc
     1141 cgaaataggc ctgacgccgc aatttgtcgg ccgcctggag cagcggctcg gccgcaaacc
     1201 ggccctgttg cattcggcgc tctccgactc ggcacgcctg gccgcgtggc gggcggcgcg
     1261 cagcggcgcc gcatccctgc tgatcggcac gcgctcggcg gtattcacac cgctggccaa
     1321 ccccgggctg ataatcattg acgaggagca cgacagctcc ttcaaacaac aggaaggcct
     1381 gcgctattcg gcccgcgatc tcggcatcgt gcgcgccaag caaatggata tacctgtcgt
     1441 gctcggttcc gcgacaccgt cactggaatc gctgcacaac tgtgaagaag gcaatttccg
     1501 gcggctcgaa ttgccgtcgc gggccggcgc ggcaaacccg ccgcgcctca ggctggtcga
     1561 taccagtcat ttgagtgcgt ccgagagtct aagcccggcg ctgaccgagg ccatgcgcgc
     1621 tcacctggat gccggcaacc aggttctgct gtttctcaac cggcgcggtt ttgccccgac
     1681 actgatttgt gtcgcgtgcg gccacatcgc cgattgcccg cgctgcgatg cgcgaatgac
     1741 cgtgcacgcg aaatctgccc agctcctttg ccaccactgt ggcaacagcc gcccgttgca
     1801 gcagcattgc agtgaatgcg gcgcggagtg ccgcccgctg gggcacggca cggagagact
     1861 ggaaaacgtc ctgctcgaca agtttccggg acagtctatc gctcgcatcg acagtgacag
     1921 cgtgcagcgc aaaggcatga tggaccaggc gctcgaactg gcgaccagcg gcgaggccag
     1981 catcctggtc ggcacccaga tgctgtcgaa ggggcatcat tttccccagc tgacgctggt
     2041 cggtgtcatc aacgccgacc aggggctgtt cggcacggat tttcgcgcgg ccgaaaagct
     2101 cgcgcagggg ctgatccagg tggcggggcg cgcaggccgg gaggatcgcg ccggcgaggt
     2161 gctgatccag acgtcgtttc caaagcacgc attctggcaa gagctgttga gcggaggtta
     2221 cccgcgcgtc gcggcggcgg cgctgcagga gcggcgcctg tcgggctggc caccctattc
     2281 gcggctcgcg ttgattcgtt cctcgtcgca ccgccgtgag gatgcgcggc gattcctgga
     2341 gcgggcgcgc agcctcgccg cgggactgca gattgccgag gtcaatttgc tgggtcccgt
     2401 gggcgcggca atggagcgtc gcgccggacg ttaccgggcc cagctgctgc tgcagagcgc
     2461 cagccgtgcg gccctgcatt cgctgcttcg ccggctgcgc cccctgctgg aggaggacaa
     2521 gagcgtcaag cgagtgcgct ggtcgatcga tatcgacccg atcgaactcg cctgatctcg
     2581 cccggtgttg atttgcgccg ggccgttcgt tcgctggcaa gggacaaggt ggcggcttac
     2641 aggtagaatt gccgccgtcg aaagaagggc aaaaccttga aacaaagcat cgcacagatt
     2701 atcgaatcca cggttcgcag cattccggaa ttggccgccc tgcccgagct ggagaccatt
     2761 gcggccgtcg tcgaacggac gcgcgattca aagcacggtg attttgcaac cagtatcgcc
     2821 atgcagctcg cgaaacccgc ccgccgcaat ccgcgtgagc tggcgcaact aatcgtcgac
     2881 cacctgccgg caaccccgct gatcgacaag gtcgagattg ccgggccggg attcatcaac
     2941 ttttatatcg gcgccggcgc ttttcacgag gagctgctgc acgttctgga ccagggcgct
     3001 gactacggca cgcaaacgtg ccgcgaaagt ccgcgctacc tgctcgaatt cgtttccgcc
     3061 aaccctaccg gcccgctgca cgtaggccat ggacgccacg catcgttcgg cgcatctgtc
     3121 ggcagtttga tgcgcgccgc cggctatgcc gtcgacctgg agtactacgt gaacgacgcc
     3181 ggccggcaaa tggatatcct cggtgccagc gtattcttgc ggctgctgca aagccgtggc
     3241 gaggacgtgg caattccggc cgccggctac cgcggcgatt acattcgtga catcgcggcg
     3301 cagatcaagg gcgtcgaggt gggcgacatc acggccgcgg cactgttaca gggactgccc
     3361 gccgatgagg cgcaaggcgg tgacaaggaa gcctacatca cggccgtgat cgatcgtgcg
     3421 caagcgttgc tcggtaccga ggctttcgcc agcctgcggc aacaatccct gagtttgatt
     3481 cgcgacgaaa tccgcgaaga cctgctcgag ttcggcgtgc gtttcgacaa ctggtattcg
     3541 gaacaaagcc tcgaagcaaa tgacctgatc gacaaagcac tggccgtgct gcaggagcgc
     3601 ggcatgcttt acaaacagga tggcgcaacc tggtttcgct caagcgactt cggcgacgag
     3661 aaagatcgtg tcgtgattcg cgaaaacggc aagaagacct atttcgcatc cgacatcgcc
     3721 tatcacttcg acaagcgaca gcgcggctac gatcacctga tcgatgtgct cggcgcggat
     3781 caccacggtt atgtggcgcg cgtgcgtgcg ggtctgcagg ctttcggtta cgcgggtgac
     3841 tcgttcgaag taccgctgat ccagttcgtc agcctgtatc gcagcggcga aaaagtcgcc
     3901 atgtccacac gttccggcga cttcgtgacc ctgcgccagc tgcgtgaaga ggtgggcaat
     3961 gatgccgcgc gatttttctt cgtcatgcgt tcgaatgacc agcatctcga cttcgacctc
     4021 gagctggcaa aatcgcggtc gaatgacaat cccgtctatt acatacagta cgctcatgcg
     4081 cgcgtcgcca gcgtgtttcg gcagctcgcg gaaaaattgc tggcctggga tcgcgaacag
     4141 gcgaggaccg gcctcgcgct gttgactgcc gagcacgaga aagcgctgat ggcaaccatg
     4201 agccgttacc ccgaagtgat cgatctcgct gcacgcaacc gtgcgccgca gcacctggtg
     4261 cattacctgc gggatctggc gaacgacttt catacttact acaacgcgca cgtcttcctg
     4321 gtggaggacg cggcgctgcg caacgctcgc ctgttattga tcgaggccac gcgccaggtt
     4381 gtcgcgaacg gcctcggcat cctgggcgtc tccgcacccg agagcatgta acccgatgac
     4441 cgcaaagaag accagccgca agaaaccggt acgcggaaac actcgcaaga agcccttgcc
     4501 aggctggcta tggctgtctt ttggcctcgc tatcggcctg tcagtggcgc tcgccatatt
     4561 cctcagcgac cagcgcaggc aaagcgcgca acacctggcg acaccgccag c
//
LOCUS       DATCZV010000015         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_530055, whole genome shotgun sequence.
ACCESSION   DATCZV010000015 DATCZV010000000
VERSION     DATCZV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530055"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VIW27_00300"
     CDS             <1..873
                     /locus_tag="VIW27_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738126.1"
                     /translation="TSRAGFDFDAIAATARIALRMLDSVIDLSHFPLPAQAEQARGTR
                     RVGLGISGLADALIMLGLHYAEPAAREQAALAMRTIRDAACLESVELARQHGAFPFFD
                     RDAYLEAAGVRGLPAPIRQAIHDHGIRNSHLTAIAPAGTISLLANNISSGIEPVFDFR
                     YRRRIRDAAGVFREYTTDDFAWRLWQQSRPAEALPEYFVKAHSLTPMAHLEMQAALQP
                     CVDSAVSKTVNVPADCGFAAFQELYREAYRLGLKGCTTFRPNEVTGSVLGGGDESLAT
                     EAMTGCCNLERESG"
     gene            complement(877..1581)
                     /locus_tag="VIW27_00305"
     CDS             complement(877..1581)
                     /locus_tag="VIW27_00305"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY5738127.1"
                     /translation="MSSEDLAQRGRRKDRDLERRRSAMIDDIEAGMAATRSLTGRERL
                     GRRVLRACRQVRREDFVPPDLRRAAFRDGALPLGYGQTISQPYIVALMTDLLDLDERS
                     RVLEVGTGSGYQAAILSCLAQRVYTIERIAQLAESARKRLRELGYDNVETRCGDGYRG
                     WPEQAPFDAIVVTAAAPVVPPMLLEQLKPGGRMVIPVGMPARHQELMLVTRDLAGEIH
                     QRSVLAVAFVPMVEDA"
     gene            complement(1578..>2119)
                     /locus_tag="VIW27_00310"
     CDS             complement(1578..>2119)
                     /locus_tag="VIW27_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY5738128.1"
                     /translation="LPNADPLDKVSIIPRGMALGMTEQIPEEEHYNLRHGYLRDRIGV
                     MLGGRAAEKLVFGEVSSGAENDLKQATRVARHMVTHWGMSERLGPVAFRRGEEHIFLG
                     REMTQQRDFSEHTARIIDDEVRGLLQGIDRQVFDLLHERRTQLDALAAALLQKETLEA
                     HEIVAILAAPEPAQAQDQP"
BASE COUNT          326 a          710 c          708 g          375 t
ORIGIN      
        1 acgtcgcgcg cgggcttcga tttcgatgcc atcgcggcga ctgcccggat cgcactgcgc
       61 atgctcgaca gtgtcatcga cctgtcgcac tttccgctgc cggcacaggc cgagcaggcc
      121 cgcggtacgc ggcgggtcgg actcggcatc agcggcctgg cggacgcgct gatcatgctc
      181 ggactgcatt acgccgaacc tgccgcgcgc gagcaggctg cgctggcgat gcgaacaatc
      241 cgcgacgccg cctgcctcga gtcggtcgaa ctggccaggc agcacggcgc cttcccgttt
      301 ttcgaccgcg acgcctacct cgaagcggcc ggcgtgcgcg gcttgccggc gccgattcgg
      361 caagcaatcc acgatcacgg cattcgcaac agtcacctga ccgcgattgc accggccggc
      421 accatcagcc tgctggccaa caacatttcc agcggcatcg agccggtgtt cgattttcgc
      481 taccggcgcc ggattcgcga cgccgcgggg gtgtttcgcg aatacacgac cgatgacttc
      541 gcctggcgcc tgtggcagca gagcaggccg gccgaagccc tgccggagta tttcgtcaag
      601 gcccattcgc tgacgccgat ggcacacctc gaaatgcagg cggcattgca accctgcgtc
      661 gacagcgcag tgtcgaagac cgtcaacgtg ccggccgatt gcggatttgc cgctttccag
      721 gagctgtacc gagaggccta ccggctcggg ctcaagggat gcacgacgtt tcgccccaac
      781 gaggtcaccg gcagcgtact cggcggcggc gacgaatccc tggctaccga ggcgatgacc
      841 ggttgctgta acctggaacg cgagagcggc tgactttcag gcgtcctcga ccattggcac
      901 gaaagccacg gcgagcacgc tgcgctggtg gatttcgcct gccaggtcgc gggttaccag
      961 catcagctcc tggtgcctgg cgggcatgcc gacaggaatc accatgcgtc cgccgggctt
     1021 gagttgttcg agcagcattg gtggcacgac gggcgcggcc gcggttacca cgatggcatc
     1081 gaagggcgct tgttcgggcc agccccggta gccgtctccg cagcgcgttt cgacgttgtc
     1141 gtaacccagc tctcgcagcc gcttgcgagc gctctcggcg agttgcgcaa tgcgctcgat
     1201 ggtgtagacc cgttgcgcga ggcaggacag gatcgcggcc tggtaaccgc tgccggtacc
     1261 gacctcgagc acgcggctgc gctcgtcgag atcgagcaaa tcggtcatca gcgcgacgat
     1321 atacggctgc gagatggttt gcccgtaacc gagcggcagc gcgccatcgc gaaatgcagc
     1381 gcggcgcagg tcgggtggca cgaaatcttc tcggcgaacc tggcgacagg cgcgcagcac
     1441 gcgccggccg agccgctcgc ggccggtcag cgagcgggtc gcagccatcc cggcctcgat
     1501 gtcgtcgatc attgcgctgc gccgacgctc caggtccctg tcctttctcc ggccgcgctg
     1561 tgccaggtct tcgctgctca tggctggtcc tgtgcctggg cgggttcggg tgcggcgagg
     1621 atggcgacga tctcgtgggc ttccagcgtt tccttttgca gcagcgcggc cgccagcgca
     1681 tcgagttgtg tgcgccgctc gtgcagcagg tcgaacacct ggcggtcgat gccctgcaac
     1741 agaccgcgga cctcgtcgtc gatgatgcgc gcggtgtgtt cgctgaaatc gcgttgctgg
     1801 gtcatctccc ggccgaggaa gatgtgctcc tcgccgcggc gaaacgcaac cgggccgagc
     1861 cgctcgctca tgccccagtg ggtaaccatg tggcgcgcca cccgggtcgc ctgcttgagg
     1921 tcgttctcgg cccccgagct gacctcgccg aaaaccagct tctcggccgc gcggccgccg
     1981 agcatgacgc cgatgcgatc gcgcaggtat ccgtggcgca ggttgtagtg ctcttcctcg
     2041 gggatttgct cggtcatgcc gagcgccatt ccccgcggaa tgatgctgac cttgtcgagc
     2101 gggtccgcgt tgggcaggc
//
LOCUS       DATCZV010000016         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_265342, whole genome shotgun sequence.
ACCESSION   DATCZV010000016 DATCZV010000000
VERSION     DATCZV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265342"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIW27_00315"
     CDS             <1..273
                     /locus_tag="VIW27_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5738129.1"
                     /translation="LHARVQEIERLTVQNARDRLVSYLLDHIVDRDGDEATIRVDLPR
                     HVIAARLSITPETLSRMLRNLVDERILTIEDRVIFVHSLSRLRPYD"
     gene            289..474
                     /locus_tag="VIW27_00320"
     CDS             289..474
                     /locus_tag="VIW27_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738130.1"
                     /translation="MSVIYFTLVGIALYLVSDWLLQRVEASAGRRFEHRSLIFFVILL
                     SLALITFEFIQRIGGTT"
     gene            474..653
                     /locus_tag="VIW27_00325"
     CDS             474..653
                     /locus_tag="VIW27_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738131.1"
                     /translation="MSEPTHDEGQSGATDQEPIPAMQKLLDNPFLLLLLGILVPAVFY
                     IIWGIIEITQIPIAE"
     gene            665..1198
                     /locus_tag="VIW27_00330"
     CDS             665..1198
                     /locus_tag="VIW27_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEY5738132.1"
                     /translation="MAITPPSERIWWKERIAKVELIWILVAFTWGLVMFFMMIYWHGA
                     GEQNLSNEAYKTTPAAFSRKAQAMIDEYTVRQENNFPVVHPPPGSDIYLIARLWQWWP
                     ILELEKDQSYRLHLSSMDWQHGFSLQPVNINVQVHPGYDMVVTIKPDRAGEYGVICNE
                     FCGIGHHNMAGRIYVVE"
     gene            1210..>2057
                     /locus_tag="VIW27_00335"
     CDS             1210..>2057
                     /locus_tag="VIW27_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5738133.1"
                     /translation="MTTSNDFRTCPTTGLRVHLGAEKLIKANAVVAVVFLAIGGLFGL
                     LVALTRWPEVHILPADWFYLALTAHGLDVLLVWIIFFEMAVLYFASSVLLGSRIAAPR
                     WAWTGFTLMLVGALVTNVAVLRGDSSVMFTSYVPMMAHWSFYLGIILFAVGALIGCFV
                     FFGTLVVAKQEKTYQGSIPLVTFGAVTAAIIAVFTIASGAIILIPTLFWSLGYISYID
                     PLVYKTIWWGMGHSSQQINVSAHVAVWYAIAAMVFGAKPLSEKVSRMAFLMYILFLQI
                     ASAHHML"
BASE COUNT          386 a          641 c          600 g          430 t
ORIGIN      
        1 ctgcatgccc gcgtccagga aatagagcgg ctgaccgtcc agaacgcgcg cgaccggctg
       61 gtcagttacc tgctggacca catcgtcgac agggacggcg acgaggcgac gatccgcgtg
      121 gacctgccgc ggcacgtcat cgccgcccgg ctgtccatta cgccggagac cctttccaga
      181 atgctacgaa acctcgtgga cgagcggatc ctgacgatcg aggaccgcgt gatattcgtc
      241 cacagcctgt cacgcctgcg gccttacgac taatcggaga ttcgagaagt gagcgtaata
      301 tatttcacgc ttgtaggaat cgcgctctat ctcgtatccg actggctgct gcagcgcgtg
      361 gaggcttccg ccggcaggcg gttcgaacac cgctcgctga ttttcttcgt gatcctgctg
      421 tcactggcat tgatcacgtt cgagttcatc caacgaattg ggggaacaac gtaatgagtg
      481 aaccaacgca cgacgagggc cagtccgggg ctacggacca ggaaccgatt ccagccatgc
      541 agaaactgct cgacaacccg tttctgctgc tgctgctcgg tatcctggta cccgccgttt
      601 tctatattat ctggggaatc atagaaatca cccagatccc aatcgctgaa tagggaggcg
      661 catcatggca attacacctc ctagcgagcg catctggtgg aaggagcgca tcgcaaaagt
      721 cgaacttatc tggatcctgg tcgccttcac gtggggcctg gtcatgttct tcatgatgat
      781 ctactggcac ggcgccggcg agcagaatct gtcgaacgag gcttacaaga cgacgccggc
      841 cgcattttcg cgaaaagccc aggccatgat cgacgagtac acggtccggc aggaaaacaa
      901 ctttcccgtc gtccatccgc cgcccggcag cgacatctac ctgatcgcga ggctctggca
      961 gtggtggccc atcctcgaac tcgaaaagga ccagagttac cggctgcacc tgtcctcgat
     1021 ggactggcag cacggttttt ccctgcagcc ggtcaatatc aacgtccagg ttcacccggg
     1081 ctacgacatg gtcgtcacga tcaaacccga ccgcgccggc gaatacggcg tcatctgtaa
     1141 cgagttttgc ggcatcggcc accacaacat ggccggccgc atatacgtag tggaatgagg
     1201 ggcggcgaaa tgactacttc aaacgatttc aggacctgcc ccactacggg gctccgggtt
     1261 cacctgggcg ccgaaaagct gatcaaggcc aacgccgtcg tcgcggtcgt gtttctcgcg
     1321 atcggcggcc tgttcggcct gctggtcgcc ctcacccgct ggccggaggt tcatatcctg
     1381 ccggccgact ggttctatct ggcgctgacc gcgcacggcc tcgacgtgct gctggtgtgg
     1441 atcatcttct tcgagatggc ggtcctctac ttcgcgtcgt cggtgctgct gggcagccgc
     1501 atcgcggcgc cgcgctgggc ctggaccggg ttcaccctga tgctcgtcgg cgccctggtc
     1561 accaacgtcg cggtgctgcg cggcgattcc agcgtcatgt tcacgtccta cgtgccgatg
     1621 atggcgcact ggagtttcta cctgggaatc attctcttcg cagtgggagc gctcatcggc
     1681 tgcttcgtgt tcttcggcac gctggtggtc gccaagcagg aaaagaccta ccagggctcg
     1741 attccgctgg tgaccttcgg cgcggtgacg gcggcgatca tcgcggtgtt cacgatcgcc
     1801 tccggcgcca ttatcctgat ccccaccctg ttctggtcgc tgggctacat cagctacatc
     1861 gacccgctgg tctacaagac gatatggtgg ggcatggggc attcctcgca gcagatcaac
     1921 gtgtcggctc acgttgccgt ctggtacgcg atcgcggcga tggttttcgg cgcgaaaccg
     1981 ctgtcagaga aagtcagccg catggccttc ctgatgtaca tcctgttcct gcagatcgcc
     2041 agcgcccacc acatgct
//
LOCUS       DATCZV010000017         3113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_1004, whole genome shotgun sequence.
ACCESSION   DATCZV010000017 DATCZV010000000
VERSION     DATCZV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VIW27_00340"
     CDS             complement(<1..591)
                     /locus_tag="VIW27_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY5738134.1"
                     /translation="MRSRLWLYAQLVRFEKPIGFYLLLWPTLWALAIAGGGAPDPWIL
                     FVFVAGSFLMRSAGCAINDYADRDLDRQVARTRERPLTSGKISSREALAVFAALSLVA
                     FALVLTLDRFTIQLSFVGIALAASYPFMKRYTYLPQVYLGAAFGWAVPMAFAAEVEAV
                     SVLAWLVFVATVIWATVYDTMYAMVDRPDDLKIGVKS"
     gene            697..1221
                     /locus_tag="VIW27_00345"
     CDS             697..1221
                     /locus_tag="VIW27_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY5738135.1"
                     /translation="MIRSYRDKTPSIGKGCYIDETAVVIGDVVLGENCSVWPLTVIRG
                     DINRIRIGANTNIQDGSVLHVTHRGEFSPEGAELVIGDEVTIGHKALLHGCRIGNQCL
                     IGMGSIITDNAIVEDRVIIGSGSLVPPGKRLESGYLYLGYPVQQKRALSERELDYFSY
                     VARHYVKMIEDYRD"
     gene            1226..1390
                     /locus_tag="VIW27_00350"
     CDS             1226..1390
                     /locus_tag="VIW27_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY5738136.1"
                     /translation="MKRWECLVCGFIYDEAAGWPEDGIAPGTRWQDVPDDWLCPDCGM
                     GKEDFEMIEI"
     gene            complement(1552..2412)
                     /locus_tag="VIW27_00355"
     CDS             complement(1552..2412)
                     /locus_tag="VIW27_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738137.1"
                     /translation="MKIDHIRTFLEISNCGNFNRAAENLNVTQSTVSARVKAMEDRFG
                     RELFTRGHSGVELTSAGHHFRQYALNIQRLWQQAQQRISLPESFSHGIGLGSQVSLWD
                     SLILEWIPWMRRHAPAVAVHVEADYSPSLMRQLSDGLLDIGVMYNPRQTPGLIIEDLL
                     QETLVLVATDRRQLSEGWVGDYVYVDWGGEFRRRHGEAFPNMDTPAISVGLGPLGLEY
                     ILQNGGSGYFPLRVVQPHIERGELYHVDGAPAMKRPAYVVYPEYARQQETLDLALDGL
                     REIARQWESP"
     gene            2529..>3113
                     /locus_tag="VIW27_00360"
     CDS             2529..>3113
                     /locus_tag="VIW27_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5738138.1"
                     /translation="MHSATTSPVSDAANAAIILVFGLFLFSIQDVIIKHFSGHYSVLQ
                     IVFARGLIAICLMLLFMRLSGTSVPLLSRRPGLMLARGLLGFSSYTTFYLAVAAMPLA
                     EVVSITFTMPLIVTAMSALLLGEKVGPRRWGAVVVGFIGVVIILSPSGEFNSLAVVFA
                     FAAALTYASQTVLTRFLGAHDHPMTIAFNAIVIFT"
BASE COUNT          614 a          972 c          932 g          595 t
ORIGIN      
        1 cgacttaacc ccaatcttca aatcatctgg gcgatcgacc atcgcgtaca tggtgtcgta
       61 gacggttgcc cagatcaccg tcgcgacaaa caccaaccag gccagcaccg acaccgcctc
      121 tacctccgcg gcaaatgcca tcggcaccgc ccaaccaaaa gcggcgccca agtagacctg
      181 cggcaaatag gtgtagcgct tcatgaacgg atagctggcg gcgagcgcga tgccgacgaa
      241 cgacagttga atagtaaagc ggtccagtgt cagcaccagc gcgaaggcga ccaggctcag
      301 tgccgcgaac accgccagtg cctcacgcga actgatcttg ccgctggtca gcgggcgctc
      361 gcgggtacgc gcgacctggc ggtcgagatc gcggtcggcg tagtcgttga tcgcgcaacc
      421 cgccgagcgc atcaggaagc ttccggcgac gaagacgaac aggatccacg ggtcgggagc
      481 gccgccgccg gcgattgcca gcgcccacag ggtgggccac agcagcaggt aaaatccgat
      541 cggtttttcg aaacgtacca actgcgcata cagccacagg cgcgagcgca gcatggacct
      601 ggcggttgac aatctggctg tagagagatg ttccaaaatc gatgcgccgc gggaaaaaat
      661 cgttactata cgcgtttctc gcgccaggaa gaaaccatga ttcgcagcta ccgcgacaag
      721 accccgagta tcggcaaggg ttgttatatc gacgaaaccg cggtcgtcat cggcgacgtc
      781 gtgctcggcg aaaactgctc ggtatggccg ctgaccgtga ttcgcggcga catcaaccgg
      841 attcgtatcg gcgctaatac caacatccag gacggcagcg tgctgcacgt aacccaccgc
      901 ggagaattca gcccggaagg cgccgagctg gtgatcggcg acgaggtgac gatcggtcac
      961 aaggccctgc tgcacggctg tcgtatcggc aaccagtgcc tgatcggcat gggttcgatc
     1021 atcaccgaca acgcgatcgt cgaggatcgc gtgatcatcg gttccggctc gctggtaccg
     1081 cccggcaagc ggctcgaatc gggttacctg tatctcggtt atccagtgca gcaaaaacgc
     1141 gcgctgagcg aacgcgaact cgactatttc agctacgtgg cgcgccacta tgtaaaaatg
     1201 atcgaggact accgggattg agccgatgaa acgctgggaa tgtctcgttt gcggcttcat
     1261 ctacgacgag gccgcgggct ggcccgaaga cggcatcgcc cccggcaccc gctggcagga
     1321 cgtgcccgac gactggctgt gtcccgactg cggcatgggc aaggaagact tcgaaatgat
     1381 cgagatttag cccgcgacta aatctcgatc atcccgcaaa gcgcgcagcg ctgaccaatg
     1441 cgccggaaag ccgagccccc cagctctaaa aataccccaa attgccgctt accccgtaat
     1501 ttgggtcaga tccaaattaa agatggcggg ataatttgga tctgacccaa attaggggga
     1561 ttcccactgg cgggcgatct cgcgcaggcc gtcgagcgcc aggtcgaggg tttcctgctg
     1621 gcgcgcgtat tcggggtaga cgacataggc cggacgtttc attgccgggg cgccgtcgac
     1681 atggtacaac tcgccgcgct cgatgtgcgg ctgcaccacg cgcagcggaa aatacccgga
     1741 gccaccattt tgcaggatgt actcgagccc cagcgggccg aggccgaccg agatcgccgg
     1801 cgtatccatg ttcggaaacg cctcgccatg gcggcggcga aattcgccgc cccagtcgac
     1861 gtaaacgtaa tcgccgaccc agccttcgct cagctggcgg cggtcggtgg cgaccagcac
     1921 cagggtctcc tgcagcaagt cctcgatgat cagcccgggc gtctgccgcg ggttgtacat
     1981 cacgccgata tccagcaggc cgtccgacag ttgccgcatc aggctcggtg aataatccgc
     2041 ctcgacgtgc accgcgacgg cgggcgcgtg gcggcgcatc cacggaatcc attcgagtat
     2101 caggctgtcc cacaggctga cctgcgagcc gaggccgatg ccgtggctga aactctccgg
     2161 cagcgagatg cgctgctggg cctgctgcca caggcgctgg atgttgagcg cgtactggcg
     2221 gaaatggtgt cccgcggacg tcaattcgac gccggagtgg ccgcgggtga acaattcgcg
     2281 cccgaaacgg tcttccatcg ccttgacccg cgcgctgacc gtcgactggg tgacgttcag
     2341 gttttcggcc gcgcggttga agttgccgca gttcgaaatc tccaggaagg tgcgaatgtg
     2401 atcgattttc acgcgtgccc tcgccaacat tgttaatcga atttttcgat tatactaacc
     2461 atttaatttc acttttcaaa tagccggcgc ctactagact gcatcctgtt gccgccagga
     2521 tactccgcat gcacagcgcc accaccagcc ccgtttccga cgccgccaat gccgcgatca
     2581 ttctggtctt cggactgttc ctgttctcga tccaggacgt catcatcaag catttcagcg
     2641 gtcactactc ggtgctgcag atcgtcttcg cgcgcggcct gatcgcgatc tgcctgatgt
     2701 tgctgttcat gcgcctgtcg ggtaccagcg taccgctgct ttcgcgccgc cccgggctga
     2761 tgctggcgcg gggattgctc ggcttttcgt cctacacgac cttttatctg gcggtcgcgg
     2821 ccatgccgct ggccgaggtg gtctcgatca ccttcacgat gccgctgatc gtcaccgcga
     2881 tgtcggcgct gttgctcggc gagaaggtcg gcccgcgccg ctggggcgcg gtggtggtgg
     2941 gtttcatcgg cgtggtgatc attctgtcac cgagcggtga attcaattcg ctggcggtgg
     3001 ttttcgcctt tgctgcggcg ctgacttacg cctcgcaaac cgtgctgacc cgttttctcg
     3061 gcgcccacga ccacccgatg accatcgctt tcaacgccat cgtgatcttt acg
//
LOCUS       DATCZV010000018         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_318595, whole genome shotgun sequence.
ACCESSION   DATCZV010000018 DATCZV010000000
VERSION     DATCZV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318595"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VIW27_00365"
     CDS             <1..666
                     /locus_tag="VIW27_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY5738139.1"
                     /translation="LRILDSKNPEMQELIEAAPALLDSLDAHSVEHFAELREILDHAG
                     VAYRVNQRLVRGLDYYCHTVFEWVTETLGAQGTVCGGGRYDGLVEQLGGKPNYAAGFS
                     MGCERLVSMLVDQGKAPEETGCDIYLVMRGDHSVAHAQRLAESLRDRLPRLQLVVHGG
                     GGSFKSQMRKADRSGASIALILGESEMAAGTVAVKYLRRDQPQEEISQADLAARLEPM
                     LEQ"
     gene            679..1302
                     /locus_tag="VIW27_00370"
     CDS             679..1302
                     /locus_tag="VIW27_00370"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5738140.1"
                     /translation="METDDEQIEKLKAWLKENGMSIVLGIVLGAGGIGGYNYWQQYQQ
                     NRAAEASVHFETLLGALRSGDGETVQRRADILVAEFESTDYAQMARLALARNYVDGGD
                     FDQAETVLQEVVGSAGQSPLAYVARTRLAAVQIQNERFEQALATLAADFPDGFVARVE
                     ELRGDALASQGQVSEARDAYRRAQGAQPGPADAAFLKQKLDDLGAPS"
     gene            1299..>2020
                     /locus_tag="VIW27_00375"
     CDS             1299..>2020
                     /locus_tag="VIW27_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5738141.1"
                     /translation="MIRALQRLAPLVLALLVGCSSEKDNAEPPAPLTDIDSPLPLIIN
                     WKRDTSGAENSAAYRLQPLLIGDRVYTIDTEGSIVCVNAADGRRIWRFETGLSAIAGL
                     GGDAGLIIASSRDGDIAAYREQEKGLQLAWKVSIDNEIRATPVLDGEQIFVRSVDGRL
                     RSLSAADGSLQWVIAHRVPALSLTGNSEPLVVGQQVFVGMDDGKLLALDRNSGEVVWE
                     ATVSLPSGRTEVERLVDVDGRFV"
BASE COUNT          374 a          609 c          696 g          341 t
ORIGIN      
        1 ctgcgtattc tcgacagcaa gaatccggaa atgcaggaat tgatcgaggc cgcgccggcg
       61 ctgctcgaca gcctcgacgc gcattccgtc gagcatttcg ccgagctcag ggaaattctc
      121 gatcatgccg gggtcgccta ccgggtcaac cagcgtcttg tcaggggcct cgactactac
      181 tgtcacacgg tattcgaatg ggtcaccgaa actctgggcg cgcagggcac ggtgtgcggg
      241 ggcggacgct acgacggcct ggtcgaacaa ctgggcggca aaccgaacta cgccgccgga
      301 ttttcgatgg gctgcgagcg gctggtgtcg atgctggtcg atcagggcaa ggcgccggaa
      361 gagaccggct gtgatatcta tctggtaatg cgcggcgatc acagcgtcgc gcatgcccag
      421 cggctggccg agtcgctgcg cgaccggtta ccgcgcctgc aacttgtcgt gcacggcggg
      481 ggcggcagtt tcaagagcca gatgcgcaag gccgacaggt cgggcgccag catcgcgctg
      541 attctcggcg aatcggaaat ggcggcaggc accgtcgcgg tcaagtattt gcgccgcgac
      601 cagccgcagg aagaaatttc tcaagcagac ttggcggctc ggctcgagcc gatgctcgaa
      661 cagtaataga ggggttaaat ggaaacagac gacgaacaga tcgaaaaact caaggcctgg
      721 ctgaaggaaa acggtatgtc gatcgtgctc ggcatcgtac tcggtgccgg cggtatcggc
      781 ggctataact actggcagca gtaccagcaa aaccgcgccg ccgaggcgtc ggtgcatttc
      841 gagaccttgc tcggtgcgct gcgatcagga gacggcgaaa ccgtgcaacg gcgcgccgac
      901 atcctggttg ccgaattcga atcgaccgac tacgcgcaga tggcgcgcct cgcgctggcc
      961 aggaattatg tcgacggcgg cgatttcgat caggcggaga cggtattgca ggaggtcgtc
     1021 ggcagcgccg gacagtcgcc gctcgcctat gtcgcgcgta cccggctggc cgcggtgcag
     1081 attcagaacg agcgcttcga gcaggcgctg gcaacgcttg cggccgactt tcccgatggt
     1141 ttcgtggccc gcgtcgagga gctgcgtggc gacgcgctgg ccagccaggg gcaggtcagc
     1201 gaggccaggg atgcctatcg ccgggcgcag ggcgcgcagc ccgggccggc cgatgcagcc
     1261 ttcctcaaac agaagctcga cgacctgggg gcgcccagtt gattcgagcg ctgcaacgcc
     1321 tggcaccgtt ggtgctggct ttgctcgtcg gttgctcgtc ggagaaggac aatgccgaac
     1381 cgccggcgcc gctcaccgat atcgacagtc cgctgccgct gattatcaac tggaagcggg
     1441 ataccagtgg tgccgagaac agcgccgcct accgcctgca accgctgttg atcggtgatc
     1501 gcgtttacac catcgacacc gagggttcga tagtctgcgt caatgccgcc gacgggcgcc
     1561 gaatctggcg cttcgagacc ggcctgtcgg cgatcgccgg actcggcgga gacgccgggc
     1621 tgatcattgc cagttctcgc gatggcgaca tcgccgccta ccgcgaacag gagaagggcc
     1681 tgcagctggc ctggaaagtg agtatcgata acgagattcg cgccacgccg gtgctcgacg
     1741 gcgagcagat tttcgtgcgc agcgtcgatg gccggctgcg cagcctgtcg gctgccgatg
     1801 gcagcctgca atgggtcatc gcgcaccgcg tgccggcgct gtcgctgacc ggcaacagcg
     1861 aaccgctggt ggtcgggcag caggtgttcg tcggtatgga cgacggcaag ctgctggcgc
     1921 tcgaccgtaa cagcggtgag gtcgtctggg aagcgaccgt cagcctgccc agcggccgca
     1981 ccgaggtcga gcggctggtc gatgtcgacg ggcgcttcgt
//
LOCUS       DATCZV010000019         6616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_318821, whole genome shotgun sequence.
ACCESSION   DATCZV010000019 DATCZV010000000
VERSION     DATCZV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318821"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..986
                     /locus_tag="VIW27_00380"
     CDS             27..986
                     /locus_tag="VIW27_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5738142.1"
                     /translation="MQVCEQNIIVIRDRDGELKAYYNVCQHRGHELLFGSGKVRTITC
                     PYHAWSYGFDGELKAARNTERMAGFDKCEFALKPVQVEVFCGLVFVNLDPEAAPLGEQ
                     AAGLEREIRDYCPRVDEVVFAQRDDYDVACNWKVLVDNFLECYHCHPAHKDFVSLVDM
                     DSYRVTTHEIYSSHVSNAAKSTDNNAYSFEKGEVDFGYAGWFLWPNLTIWIYPGEPNI
                     STLQMIPAGAGRTIEHQDWYLPNPEPSRQQRDAMDYQRNVLQPEDVGLCESVFRGLQS
                     RGYNQGRFVVDAERSELSEHGVHHFQRLYVDAMNSDIGNSGAE"
     gene            1211..2380
                     /locus_tag="VIW27_00385"
     CDS             1211..2380
                     /locus_tag="VIW27_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738143.1"
                     /translation="MFTMDIRRYIPDHAATSVPLLALLFTLHYFVAGDDRAGPGSEVV
                     DGAPAFYPHWQMPAERDPLDIDASLQRLGLDDAQRDRMIRQLLDDGRFQQARTQLLQV
                     AAAAVRRQEQARLGDSLMLLGEVAIEQQELSAAEIYLQEALYLSMERDDPLATARAYR
                     LLGQLNIRSRELARRASNGYDELWQARNSIARGHYRGVYGQLEQVIRDNLEIRRYGAA
                     ADAWEAMAAYHDKLHDDYQAQLARVEAAKLFASTGQTTHVDRLLDTVDRGVVGDSGLA
                     EIETEIRRLLEQYHQDVIKSSQARDYQMLYHHYLRLGQVERAWQFRIKSSETLAGASE
                     RLLYQRQADVIAVLFNSNFAMDRARRYLDKAGMIYDDNGMPELLSQTRQLEMQIF"
     gene            2622..3332
                     /locus_tag="VIW27_00390"
     CDS             2622..3332
                     /locus_tag="VIW27_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEY5738144.1"
                     /translation="MTDYEDDDEYEDDLDLRTLPDDELVEQMHDDLYNGLKEEVAEGT
                     EILLERGWDANKVLNEALVAGMTIVGIDFRDGILFVPEVLMSANAMKAGMAILKPLLS
                     QSDEPTAGKMVIGTVKGDIHDIGKNLVCMMMEGAGFEVVDLGINTPVEDYIAALEEHK
                     PEILGMSALLTTTMPYMKVVIDTMKEQGIRDDYIILVGGAPLNQEFGEAIGADAYCRD
                     AAVAADTAKKLVAERRAG"
     gene            3370..3984
                     /locus_tag="VIW27_00395"
     CDS             3370..3984
                     /locus_tag="VIW27_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020288116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY5738145.1"
                     /translation="MPDSAPILVITCAAIAREVNEIKKLGQWQQMDLQAITADLHSRP
                     EKIPAAVAERIDRARDRYRHIFVAYGDCGTSGALDRVLEERGVKRLPGAHYYDFLAGH
                     DNYRQLQDDEPGTFYLTDFLAQHFRRLVIEVLGIDRHPELKEMYFGNYTRLVYLAQTE
                     SDELTALAGEAAAELGLRFERVFTGMGEMLPSLDAAMQEAMCRS"
     gene            3972..4289
                     /locus_tag="VIW27_00400"
     CDS             3972..4289
                     /locus_tag="VIW27_00400"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY5738146.1"
                     /translation="MPQLKIVYWRDIPGQVVIREGRRNTRLRLSPRFSKAIERAAYRL
                     KKKQQDALFEPWRDVAQPLDGADIRRQARQLVQQLETQYTQEVLETLIRASGVDENRG
                     CAN"
     gene            4301..4771
                     /locus_tag="VIW27_00405"
     CDS             4301..4771
                     /locus_tag="VIW27_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5738147.1"
                     /translation="MYSVRLWSVTHARKLDALYRAFEPMLIKLHPLLKWIGYTRLEKP
                     VRMAEHAIKGFMFDCQMCGKCALSATGMSCPMNCPKSIRNGPCGGVRLDGTCEVKPEM
                     RCVWVEAWRGSPQMGDPAAIQQVQLPVDNLLKGSSSWLRVVRHEHAAHAAQEKS"
     gene            4768..5793
                     /locus_tag="VIW27_00410"
     CDS             4768..5793
                     /locus_tag="VIW27_00410"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5738148.1"
                     /translation="MTSTPEPHLPQEPNLGLPLPGKKGHVSLGRLERVLRAGKFAVTA
                     ELAPPDSANAEDVFERARMFDGYVDGMNATDGSGANCHMSSVAMCALLTAAGYSPVLQ
                     ISCRDRNRIAIQGDVLGAAAMGVANVLCLSGDGVQVGDHPEAKPVFDLDSMSLLETIR
                     TMRDESRFLSGRPITSPPQMFLGAAENPSAPPVDFRPQRLAKKIAAGADFIQTQYVFD
                     IVQLREFIARAGDLGLLDKVFILPGVGPLASARTATWIRNNVPGIHIPDEIIKRLEGA
                     SEPKQEGRRICVELIQQIREIEGVSGVHIMAYRQEEAVGEIVQQSGVLDGRVPWYPGC
                     ELKSGTA"
     gene            5843..>6616
                     /locus_tag="VIW27_00415"
     CDS             5843..>6616
                     /locus_tag="VIW27_00415"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020058296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5738149.1"
                     /translation="MTETVVSSANRELVIGFERPFVIIGERINPTGRKIMAEEMKNGD
                     YSRVESDAIAQVEAGAHMLDVNAGIPLADEPRILAEAVQLVQSLTDVPLSIDSSIIDA
                     LEAGLSVYQGKALVNSVTGEDESLERVLPLVKKHGAAVVAISNDETGISEDIDVRYNV
                     AKKIVERAADHGIPREDVVVDPLVMPIGAINTAGREVLKLIHRLRTELKVNTTCGASN
                     ISFGLPNRHALNGHFLAMASLAGMTSAIMNPLHKEELDGI"
BASE COUNT         1293 a         2088 c         2086 g         1149 t
ORIGIN      
        1 ccggctgacc cggctgctac ctgaccgtgc aggtctgcga gcagaacatc atcgtcattc
       61 gcgacaggga tggcgaactc aaggcttact acaatgtctg ccagcatcgc ggccacgaac
      121 tgctgttcgg cagcggcaag gtgcgcacca tcacctgccc gtaccatgcc tggtcctacg
      181 gtttcgacgg cgaactgaag gcggcgcgca ataccgagcg catggccggc ttcgacaagt
      241 gcgagttcgc gctgaagccg gtgcaagtgg aagttttctg cggcctggtg ttcgtcaacc
      301 tcgaccccga agcggcgccg ctcggcgagc aggcggccgg cctggaaagg gagattcgcg
      361 attactgccc gcgcgtcgac gaggtcgtgt tcgcgcagcg cgacgattac gacgtcgcct
      421 gcaactggaa agtgctggtc gacaattttc tcgaatgtta tcactgccac ccggcgcaca
      481 aggatttcgt cagcctggtc gacatggaca gctaccgggt gacgacccat gaaatctact
      541 ccagccacgt ttccaacgcc gccaaatcga ccgataacaa tgcctacagt ttcgagaaag
      601 gcgaggtcga cttcggctac gcgggctggt tcctgtggcc caacctgacg atctggatct
      661 accccggcga acccaacatc tcgaccctgc agatgatccc ggccggcgcc gggcgcacca
      721 tcgagcacca ggactggtac ctgccgaatc ccgagcccag ccgccagcaa cgcgacgcga
      781 tggattacca gcgcaacgtg ctgcagcccg aggacgtcgg cctctgcgaa agcgtgtttc
      841 gcggcctgca gtcgaggggc tacaaccagg ggcgtttcgt cgtcgatgcc gagcgcagcg
      901 aactgtccga gcatggcgtg caccatttcc agcgactata tgtcgacgcg atgaactccg
      961 acatcggaaa ctcgggggca gagtaaaatt tccatcaacc cccggctatt gccctcccca
     1021 ggcggcgaaa tcttgcttac gcagggattt cgtgccgctt tactcgcagt gacgacgagc
     1081 ggggaatatt tactctgacc ccctggaaac gaccttgccg aagccgtcag ttacgcgcat
     1141 agccaactcc cggtgcaagc cagaagttat ttcccgacat ttgcattacc ccggtagcgg
     1201 cgtaaactcg atgtttacga tggacattcg tcgttacatc cccgatcacg cggcgaccag
     1261 cgtaccgctg ctggcgctgt tgttcacgct gcattacttt gttgccggcg acgatcgcgc
     1321 cgggcccggc agcgaggtgg tcgacggcgc cccggccttt tacccgcact ggcagatgcc
     1381 ggcggagcgc gatccgctcg acatcgatgc cagcctgcag cgcctcggtc tcgacgatgc
     1441 gcagcgtgac cgcatgattc gccagctgct cgacgacggc cgcttccagc aggcacgaac
     1501 ccagttgttg caggttgccg ccgccgcggt gcgccgccag gagcaggcgc gcctcggtga
     1561 ctcgttgatg ctgcttggcg aggtggcgat cgagcagcag gaattgtcgg ccgcggaaat
     1621 ttacctgcag gaggcgcttt acctgtcgat ggagcgcgac gatccgctgg ccaccgcgcg
     1681 cgcctatcgc ctgctggggc agctgaacat tcgctcccgc gaactggcgc ggcgcgcgtc
     1741 gaacggctac gacgaactct ggcaggcgcg caactcgatc gcccgcggtc attatcgcgg
     1801 cgtctacggc cagctcgagc aggtgattcg cgataacctc gagattcgcc gctacggcgc
     1861 ggccgccgac gcctgggagg cgatggctgc gtaccacgac aagctgcacg acgactacca
     1921 ggcgcagctg gcgcgcgtcg aggccgccaa gctgttcgca tcgaccgggc agaccacgca
     1981 cgtcgaccgg ctgctggaca cggtcgaccg cggcgtggtc ggcgacagcg gcttggccga
     2041 gatcgagacc gaaatccgcc gcctgctcga acagtaccac caggacgtga tcaagtcgtc
     2101 gcaggcgcgc gactaccaga tgctctacca ccattacctg cgtctcggcc aggtcgagcg
     2161 cgcctggcaa ttccgcatca agtccagcga aacgctggcc ggcgccagcg agcgcctgct
     2221 ctaccagcgc caggcggatg tcatcgcggt gctgttcaat tccaatttcg cgatggacag
     2281 agcccgcagg tacctcgaca aggcgggcat gatctacgac gacaacggca tgccggaatt
     2341 gttgtcgcaa acccgccagc tggaaatgca gattttctag ccgcccttgg cgtttcgccg
     2401 ccgtgctcga ggccgccacg cggcggtttt acgcggtttt gccgggggtc tgcgcgaatc
     2461 ctgaaaaccg attaataatc aacgatatcg ccggattgtg agtgtcgcgt taaggcaatg
     2521 ggaagtcgct gcaacgccag accccaaagc gcttaaaacc tattctgcgc gttctatggc
     2581 cgctctttgg cggttgccga ctgcacgagg aatcccttca aatgactgac tacgaagacg
     2641 acgacgaata cgaagacgac ctggatctgc ggacgttgcc ggacgacgag ctcgtcgagc
     2701 agatgcacga cgacctgtac aacggtctga aggaagaggt cgccgagggt accgagattt
     2761 tgctggagcg cggctgggat gccaacaagg tgctcaacga ggcgctggtc gccggcatga
     2821 ccatcgtcgg catcgatttc cgcgacggta tcctgttcgt gcccgaggtg ctgatgtcgg
     2881 ccaacgcgat gaaggccggc atggcgatcc tcaagccgtt gctgtcgcag tccgacgagc
     2941 cgaccgccgg caagatggtg atcggcacgg tcaagggcga tatccacgac atcggcaaga
     3001 acctggtctg catgatgatg gaaggcgccg gcttcgaggt cgtcgacctc ggcatcaaca
     3061 cgccggtcga ggattacatc gccgccctcg aggagcacaa gcccgaaatc ctcggcatgt
     3121 ccgcgctgct gaccacgacc atgccgtata tgaaagtggt catcgatacg atgaaggagc
     3181 agggtattcg cgacgattac atcatcctcg tcggtggcgc gccgctgaac caggaattcg
     3241 gcgaagccat cggcgccgac gcctattgcc gcgatgccgc agtcgccgcg gataccgcca
     3301 agaaactggt cgccgaacgg cgcgcgggct gagacaagcg tcgcgagcgc cgctgagtgt
     3361 cgaaacgaca tgcccgattc agccccgata ctggtgatca cctgcgccgc gatcgcccgc
     3421 gaagtcaatg aaatcaagaa gctcgggcag tggcagcaaa tggacttgca ggcgatcacc
     3481 gccgacctgc attcgcggcc ggaaaaaatt cccgccgcgg tagccgagcg tatcgaccgc
     3541 gcgcgcgacc gctatcggca tatcttcgtt gcctacggcg attgcggcac cagcggcgcg
     3601 ctcgaccggg tgctcgagga gcgcggcgtg aaacgcctgc ccggcgcgca ctactacgat
     3661 tttctcgccg gccacgacaa ttaccggcag ctgcaggacg acgaacccgg gaccttttac
     3721 ctgaccgatt ttctggcgca gcattttcgg cgcctggtga tcgaggtcct cggcatcgat
     3781 cgccaccccg agctcaagga aatgtacttc ggcaattaca cccgcctggt gtacctggcg
     3841 cagaccgagt ccgacgagtt gacggcgctc gccggcgagg cggccgccga gctcgggctg
     3901 cgcttcgagc gcgtgtttac cggcatgggc gaaatgctgc catcgctcga cgccgccatg
     3961 caggaggcga tgtgccgcag ttgaaaatcg tctactggcg cgatattccc ggccaggtgg
     4021 tgattcgcga gggccgccgc aacacgcgcc tgcggttgtc gccgcgcttc tcgaaagcga
     4081 tcgagcgggc ggcctaccgg ctgaaaaaaa agcagcagga cgcgctgttc gagccatggc
     4141 gcgatgttgc ccagccgctc gatggcgccg atattcgccg gcaggcgcgg caactggtgc
     4201 agcaactgga aacgcagtac acgcaagagg tactggagac tttaatacgg gccagcggtg
     4261 tcgacgagaa ccgcggctgc gcaaactgaa cggagcgatc gtgtattcgg tacgtctatg
     4321 gtcggtgacc cacgcgcgca agctcgatgc gctgtatcgg gctttcgagc ccatgctgat
     4381 caagctgcat ccgctgctca agtggatcgg ttacacgcga ctcgagaagc cggtgcgcat
     4441 ggccgagcac gcgatcaagg gcttcatgtt cgattgccag atgtgcggca aatgcgcgct
     4501 ctcggcgacc ggcatgtcgt gcccgatgaa ttgcccgaaa tcgatacgca acgggccctg
     4561 cggcggagtg cgcctcgacg gtacctgcga ggtcaagccg gagatgcgtt gcgtgtgggt
     4621 agaagcctgg cgcggcagcc cgcaaatggg cgatccggcg gcgattcaac aggtacagct
     4681 gccggtcgat aacctgctga agggcagctc gtcgtggttg cgggtggtgc gccacgagca
     4741 tgccgcgcac gccgcgcagg aaaagtcatg acttcgacgc cggaaccgca tctgccgcag
     4801 gagccgaacc tcggtctgcc gctgccggga aaaaagggcc acgtctcgct gggccgcctg
     4861 gagcgcgtgc tgcgtgcggg caagttcgcg gttaccgccg agctggcgcc gccggactcg
     4921 gcgaacgccg aggacgtctt cgaacgcgcg cggatgtttg acggttacgt cgacggcatg
     4981 aacgccaccg acggctcggg tgccaactgc catatgtcga gcgtggcgat gtgcgcgctg
     5041 ctgaccgcgg cgggttactc gccggtattg caaatctcct gtcgtgaccg caatcgcatc
     5101 gccatccagg gcgacgtgct cggcgccgcg gcgatgggcg tcgccaacgt gctgtgcctg
     5161 tccggcgacg gtgtgcaggt cggtgaccac ccggaagcca agccggtatt cgacctcgac
     5221 agcatgtcgc tgctggaaac gatccgcacc atgcgcgacg aatcgcgttt cctcagcggc
     5281 cggccgatca cctcgccgcc gcagatgttt ctcggtgcgg cggagaaccc gagcgcgccg
     5341 ccggtggatt ttcgcccgca gcggctggcg aagaaaatag ccgccggcgc ggatttcatc
     5401 cagacgcaat acgtgttcga tatcgttcag ctcagggagt tcatcgcccg tgccggcgac
     5461 ctcggcttgc tcgacaaggt attcatactc cccggcgtcg gcccgctggc ctcggcgcgc
     5521 accgccacct ggattcgcaa caatgtgccg ggcattcata ttccggatga aatcatcaag
     5581 cggctcgagg gcgccagcga gccgaagcag gaaggccgca ggatctgcgt cgaattgatc
     5641 cagcaaatca gggaaatcga gggcgtcagt ggcgtccaca tcatggctta ccgccaggaa
     5701 gaagcggtgg gcgaaatcgt acagcaatcc ggtgtgctcg acgggcgcgt gccctggtac
     5761 ccgggttgcg aattgaaatc cggcacggcc tgacgccgcg tccaacaata gcgaagtatt
     5821 ttgatattca ggggatacat ccatgactga gacagttgtc agttcggcca acagagaact
     5881 ggtcatcggc ttcgagcgtc cgttcgtcat catcggcgag cgcatcaacc cgactggccg
     5941 caagatcatg gccgaggaaa tgaagaacgg cgattacagc cgcgtcgaga gcgatgccat
     6001 cgcgcaggtc gaagccggcg cgcacatgct cgacgtcaac gccggtattc cgctcgccga
     6061 cgagccgcgt atcctcgccg aggccgtgca gctggtgcaa agcctgaccg acgtgccgct
     6121 gtcgatcgac tcgtcgatca tcgacgcgct cgaggccggg ctgtcggttt accagggcaa
     6181 ggcgctggtc aactcggtga ccggcgagga cgagagcctg gagcgcgtgc tgccgctggt
     6241 gaaaaagcac ggtgccgcgg tggtcgcgat ttccaacgac gaaaccggta tttccgagga
     6301 tatcgacgtg cgctacaacg tcgccaaaaa aatcgtcgag cgcgccgccg atcacggcat
     6361 tccgcgcgag gacgtggtcg tcgatccgct ggtcatgccg atcggcgcga tcaacaccgc
     6421 cggccgcgag gtgctgaaac tgatccaccg gctgcgtacc gagctcaagg tcaataccac
     6481 ctgcggcgcg tcgaacatct ccttcggtct gcccaaccgc cacgcgttga acggccattt
     6541 cctcgccatg gcctcgctcg ccggcatgac ctcggcgatc atgaatccgc tgcacaagga
     6601 agagctcgac ggcatc
//
LOCUS       DATCZV010000020         7808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_54344, whole genome shotgun sequence.
ACCESSION   DATCZV010000020 DATCZV010000000
VERSION     DATCZV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54344"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..369
                     /locus_tag="VIW27_00420"
     CDS             118..369
                     /locus_tag="VIW27_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY5738150.1"
                     /translation="MALMITDECINCDVCEPECPNDAISPGEEYYVIDPLLCTECVGH
                     FETSQCVEVCPVDCIPLNPDYVETREQLMLKYQHLTGND"
     gene            complement(384..2051)
                     /gene="ggt"
                     /locus_tag="VIW27_00425"
     CDS             complement(384..2051)
                     /gene="ggt"
                     /locus_tag="VIW27_00425"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5738151.1"
                     /translation="MFDYRHTRVLVLGISLLHAAAAGAVPAEGTRIMISAPSAYAVET
                     GKAIAAEGGNLVDVAVAVGFTLAVTNPSNASLGGGGFALVSMGGDVEVLDFREMAPAA
                     TSPGFYLELAKGASWNGGTAVGVPGVPAGLWALHKRYGKLGWSRLLERAIQLADDGIE
                     FSGTESRYSESQRERFNAAGMWHFYRATGEPYRPGEILRQPALARALAKLRNHGPAGF
                     YEGDVARDIAASVQAAGGVLSVDDLAGYEVRWLQPLVTEFRDHRVYMMPPPSSGGVVI
                     KTALELIDRVGVGERAPLSVDEIHLLAEVLNRSFRGRALLGDPDFHDNPFDRILSADY
                     LAEMARSIDVERAVQLQPLLEKKPDDSNQTTQFSILDADGNAISLTVTLNGSYGSGVV
                     SEKYGISLNNEMDDFTTRPGEANAFGLVQGLGNVVEPGKRPLSSMSPTLVAKDGRVVM
                     TLGAAGGPRIISSVVQTIYRVLVTGLDLDRAVQFPRVHHQFLPNKLYMDEFKFSPEVI
                     AGLQARGHETVEQRPAYLGRIKAIRLNDRNYLEAAYDNRSEGAVGGY"
     gene            complement(2101..2859)
                     /locus_tag="VIW27_00430"
     CDS             complement(2101..2859)
                     /locus_tag="VIW27_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5738152.1"
                     /translation="MSIHALDSQADALTRARIDLAAALRLAVMHELEEGIDNHFTVTV
                     PGHDDRFLVLPFGLHWSEARASDLIVFDEAGETLDGDGIVELSAQCIHAPIHRISGRR
                     VVFHTHQHWALALNMLRDNRLIPASQTAAFFDGNIAYDDHYQGLAGSLDEGERLASLI
                     GDCEVVFLKNHGVLVASNTVARAYRLLYLLERVCRAQLCALGSGQKIQPIAPEIVAGI
                     RAPNPDDRHQGQRDRLYFEAMKRVLDRQMPGYAD"
     gene            complement(2963..5041)
                     /locus_tag="VIW27_00435"
     CDS             complement(2963..5041)
                     /locus_tag="VIW27_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738153.1"
                     /translation="MSDDEARLRAQLEAHYFNTSTVDEIVARLQALEPRWRGFVSRWV
                     LSVARTEPEIGYQFARLAGEVLQRVGRKDCEHWLQAAFDVYFEQGLEPAIRALQDFDK
                     FIAARHKRWRAVELSSVRRLLQHFLHGLDGRRLQIETGDQACTDSEILYLPATIDQFP
                     DRQQNFACYRAQAVHLWAQCWYGTWRADSIDEIPREEPMLRTFHFLETLRLDACIARD
                     YPGLARQLCALQSPIPAIWQPLQAQLAAPRSSVRDAIHLSDDHWDKPRPAPFVYQGKL
                     DPDRVLATLRARLQRERQQLRQALQQLAEETTAEPGPEVDKPAGEGLQAAPVLNQDSG
                     ASIEFRFGGRPLPADEDVDELMVSILQDLGELPREYLSAADHADYRADLDPGLQAAAE
                     TATAGPAPQANAFLYDEWDCTRGAHRKRWCTVRERDIEPDRTSGFVAETLHKYHGHIK
                     SLRRGFEAMRDEYRMLRKQPDGGSLDFDALVEAIADTRSGMEMSSRIYRKPHRVERDI
                     AVILMVDMSGSTKGWINRAQREALILLAEALSALDDRYAIYGFSGNTRKRCEIFRIKT
                     FDESYDDEIRARIAAVAPQDYTRMGATIRHLTGLLERVSARTRLLVTLSDGKPDDYDA
                     YHGEYGIEDTRMALFEARRRGIHPFCITIDEQARDYLPHMYGDSSYLLIDDVARLPYR
                     LSEIYRSLTT"
     gene            complement(5038..5847)
                     /gene="mutM"
                     /locus_tag="VIW27_00440"
     CDS             complement(5038..5847)
                     /gene="mutM"
                     /locus_tag="VIW27_00440"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY5738154.1"
                     /translation="MPELPEVETTRRGIEPHVRGQIIERVIVRESRLRWPVSREVPTL
                     GGQRIVDLRRRGKYLIMQLQRGNLIWHLGMSGSMRVLATGSPGEDHEHVELQLANRQS
                     LKFRDPRRFGALLYCAGDPLAHRLLRDLGPEPLSAEFDADYLYRRCRGRRVAIKGLLM
                     NSHIVVGVGNIYASEALYRAGIRPTRAAQRISKARIAGLVEAIRATLAEAIEHGGTTL
                     QDFVQADGRPGYFRHELRVYGNTAQCQRCGEPIRHIVQAQRSSYYCPVCQT"
     gene            complement(5857..6642)
                     /locus_tag="VIW27_00445"
     CDS             complement(5857..6642)
                     /locus_tag="VIW27_00445"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY5738155.1"
                     /translation="MRILLSLVLLLACSGPRAQQPLPRLELGIGLIALNAPDYRGSAE
                     SSNYLLPVPYVKYRGHRLRVDEGADGIIFETPDLLLTLSGNLSLPADEDTPERDGMDK
                     LEALVEIGPSLNWRFLHLRHSDLWLDLPLRLAYTLNADFEHVGYVFQPRLSWRRPATR
                     LGEWKLRLNFGPLYSSDEYHDYFYSVSTTDATATRPAFSAEGGFSGYRTEFTYSRRIG
                     GYWLGGFVRYDSLRGSQAEDSPLVSETATWMGGIALAWVFHED"
     gene            complement(6626..7480)
                     /locus_tag="VIW27_00450"
     CDS             complement(6626..7480)
                     /locus_tag="VIW27_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY5738156.1"
                     /translation="MLSRRWLVNGLLILVIAVLATLGYRLEVEPEPPSQPAHAGFDTA
                     AVEAIEVRTADQQLALQKHASGWRLAAPLRWPADRANVERLFRIVDTGAAEPLQEAAI
                     DLARFGLDEPPAQLQLGQLSIRFGSVNNIGERRYTLIDSALYLLPDVHLPLILQGISG
                     FIDQRLLPPDFEITTLSLPGLELRRDAGGAWQAGERGDLNAETLVALVADWQHLEATR
                     IGSYQDPRAPTGRIVAGLSDGEHIELLLLATDPEIIIANPALGLQYHLRGEYFDRLFT
                     PLGDENPA"
     gene            complement(7480..>7808)
                     /locus_tag="VIW27_00455"
     CDS             complement(7480..>7808)
                     /locus_tag="VIW27_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEY5738157.1"
                     /translation="PLDLALTLTRQRDDDEQAQMQRVAVIGDADFLANQYLGNGSNLE
                     IGVNLVNWLSHDDGLISISPRPAPDTRLELSAAQQLGIAIFFLILLPLSLLGGGLRIW
                     LKRRKL"
BASE COUNT         1350 a         2449 c         2625 g         1384 t
ORIGIN      
        1 gggaaatcgc gcggctcgac ggcgacgtgt cggaattcct gcatcccgta atcctgcagc
       61 gcctgaccga caagctgcag gcgcagaagt aattttcctg gctggaggtc agaaaccgtg
      121 gcattgatga ttaccgacga atgtatcaac tgcgatgttt gcgagccgga gtgccccaac
      181 gatgcgattt cgcccggtga ggaatactac gtgatcgacc cgctgctgtg caccgaatgc
      241 gtcggccact tcgagacttc gcaatgcgtc gaagtgtgcc cggtggactg cattccgctg
      301 aacccggact acgtggaaac ccgcgaacag ctgatgctga agtaccagca cctgaccggt
      361 aacgactgac ccggtaacgc gtttcagtag ccgccgaccg cgccctcgct gcggttgtcg
      421 taggcggcct cgaggtagtt cctgtcgttc aggcgtatcg ccttgatccg tcccaggtag
      481 gcgggtcgct gctcgaccgt ttcatgccct ctggcttgca ggcccgcgat cacctcggga
      541 gagaacttga actcgtccat gtacagcttg ttgggcagga actggtggtg gacgcgcgga
      601 aactgcaccg cgcgatcgag atcgagcccg gtcaccagca cgcggtaaat ggtctgcacc
      661 acgctgctaa tgatgcgcgg gccgccggcc gcgccgagcg tcatgacgac gcggccgtcc
      721 tttgcgacca gggtcgggct catcgagctg agcggccgct tgccgggttc gacgacgttg
      781 ccgagcccct gcaccagccc gaaagcattg gcttctcccg ggcgggtggt gaaatcgtcc
      841 atctcgttgt tcagcgagat accgtacttt tccgagacca cgccggaacc gtaactgccg
      901 ttgagcgtga ccgtcagcga aatcgcgttg ccgtcggcgt cgagaatcga gaattgcgtg
      961 gtctggttcg aatcgtcggg ctttttctcc agcagtggtt gcaactggac cgcgcgctcg
     1021 acgtcgatac tgcgtgccat ttcggcgaga tagtctgccg acaggatgcg atcgaaaggg
     1081 ttgtcgtgaa aatccgggtc gccgagcagc gcgcggccgc gaaacgaccg gttcagtact
     1141 tcggccagca agtgaatttc gtcgacgctg agcggcgcgc gttcgccgac gccgacccgg
     1201 tcgatcaatt cgagcgcggt cttgattacc acgccgccac tgctaggcgg cggcatcatg
     1261 taaacgcggt gatcgcgaaa ctcggtaacc agcggttgca gccagcgcac ctcgtagcca
     1321 gcgaggtcgt cgacgctgag cacgccgccg gcggcctgta ccgacgcggc gatatcgcgc
     1381 gccacgtcgc cctcgtagaa tcccgccggc ccgtgattgc gcaacttcgc cagggccctg
     1441 gcaagcgccg gctgccgcag gatttcgccg ggccggtaag gctcgcctgt cgcgcggtaa
     1501 aaatgccaca tgccggcggc attgaagcgc tctcgctggg attccgagta acgcgactcg
     1561 gtgccggaaa actcgatgcc gtcgtcggcc agctgaattg cgcgctccag caggcgcgac
     1621 cagccgagct tgccgtagcg cttgtgcagc gcccacagcc cggctggtac gcccggtaca
     1681 ccgaccgcgg tgccgccatt ccacgaggcg cccttggcga gttccaggta gaatcccggc
     1741 gaggtggcgg cgggtgccat ctcgcgaaaa tcgagtacct cgacgtcacc acccatgctg
     1801 accagtgcga aaccgccgcc accgagcgat gcgttcgacg gattggtgac cgccagcgtg
     1861 aagccgaccg cgaccgcgac gtcgaccagg ttgccgccct cggccgcgat cgccttaccg
     1921 gtctcgaccg cgtaagccga cggcgcggaa atcatgattc gagtgccctc ggcgggcact
     1981 gcgcctgccg cggcggcgtg cagcagcgaa atccccagta ctagaacgcg cgtgtgtcgg
     2041 tagtcgaaca tgatctggcc ctgcggtagt tttccagcat tgaaaggagg gtcgggcggc
     2101 tcagtcggcg taacccggca tttgccgatc gaggacgcgt ttcatcgcct cgaagtaaag
     2161 cctgtcccgt tgtccctggt gacgatcgtc gggattcggt gcccgaatgc cggcgacgat
     2221 ttcgggggcg atcggctgaa tcttttgccc gctgccgagc gcgcacagct gcgcgcggca
     2281 cacgcgctcg agcaggtaca gcaaccggta agcgcgcgcg acggtattcg atgccaccag
     2341 tacgccgtgg ttcttgagga atacgacctc gcagtcaccg atcagcgacg ccagccgctc
     2401 accttcgtcg aggctgccgg cgagaccctg gtaatggtca tcgtaggcga tattgccgtc
     2461 gaaaaaggcc gcggtctggc tggccgggat caggcgattg tcccgcagca tgttgagcgc
     2521 cagcgcccag tgctggtgag tatgaaacac cacacggcgg ccgctgatgc gatgaatcgg
     2581 cgcgtgtatg cattgcgccg agagttcgac gataccatcg ccgtcgaggg tttcaccggc
     2641 ctcgtcgaag acgatcaggt cgctggcgcg agcttccgac cagtgcaggc cgaacggcag
     2701 caccaggaaa cggtcgtcat gacccggaac cgtgaccgtg aagtggttgt cgatgccttc
     2761 ctcgagttcg tgcatgaccg cgagccgcag cgccgcggcg agatcgatcc tggcccgggt
     2821 gagcgcgtcg gcttgcgaat cgagtgcgtg aatggacatg atggcggcct ccgcaatgcg
     2881 agaatcgcct gatgataatg gatttgcgcg gccagcgaaa atcgcggcgg gcgataagcg
     2941 ggatcggggc gccggcgatc ggctaggtcg tcaggctgcg gtaaatctcg gacagccgat
     3001 agggcagcct ggcgacatcg tcgatcagca ggtaactgga atcaccgtac atgtgcggca
     3061 ggtaatcgcg cgcctgttcg tcgatggtga tacagaacgg gtggataccg cgccggcggg
     3121 cctcgaacag cgccatgcgc gtatcctcga tgccgtattc gccgtggtag gcgtcgtaat
     3181 cgtcgggctt gccgtcggac agcgtcacca gcagccgggt acgcgcactg acgcgttcga
     3241 gcaggccggt caggtggcga atcgttgccc ccatgcgcgt ataatcctgc ggtgcaaccg
     3301 cggcgatgcg cgcgcggatc tcgtcgtcgt aggattcgtc gaaggtcttg atacggaata
     3361 tttcgcagcg cttgcgggta ttgccggaaa agccgtagat cgcatagcga tcgtcgagcg
     3421 cgcttagcgc ctcggcgagc aggatcagcg cctcgcgctg ggcgcggttg atccagccct
     3481 tggtcgagcc gctcatatcg accatcagga tgaccgcgat gtcgcgttcc acgcggtgcg
     3541 gcttgcggta gatgcgggaa ctcatctcca tgccgctgcg ggtgtcggcg atcgcctcga
     3601 ccagggcatc gaaatcgagg ctgccgccgt cgggctgttt tctaagcatg cggtactcgt
     3661 cgcgcatcgc ctcgaagccg cgccgcagcg acttgatgtg gccgtggtac ttgtgcagcg
     3721 tctcggcgac gaagccactg gtccggtcgg gctcgatatc gcgctcgcgt accgtgcacc
     3781 agcgcttgcg gtgcgcgccg cgcgtgcaat cccactcgtc gtagaggaat gcgttcgctt
     3841 gcggcgcagg gccagccgtc gccgtttcgg ctgccgcctg caatccggga tcgaggtcgg
     3901 cgcgataatc ggcgtggtcg gcggcagaca ggtattcgcg cggcaactcg ccgaggtcct
     3961 gcaggatcga gaccatcaac tcgtcgacgt cttcgtcggc cggcagcggg cggccgccga
     4021 accgaaactc gatacttgcg cccgagtcct ggttgagcac cggcgcggcc tgcaatccct
     4081 cgccagccgg tttatcgacc tcgggtcccg gctcggcggt ggtctcctcg gccagctgtt
     4141 gcagcgcctg gcgcagttgc tgccgctcgc gttgcagccg cgcgcgcagc gtcgccagca
     4201 cgcgatcggg atcgagctta ccctggtaga cgaaaggtgc cgggcgaggt ttgtcccagt
     4261 gatcgtcgct caggtgtatc gcatcgcgca cgctgctgcg tggcgctgcg agctgtgcct
     4321 gcagcggctg ccagatcgcc gggatcggcg attgcagcgc gcacagctgg cgcgcgagcc
     4381 ccgggtaatc gcgcgctata caggcgtcaa ggcgcagggt ttcgaggaag tgaaaggttc
     4441 tcagcatcgg ttcctcgcgc ggaatctcgt cgatgctgtc ggcgcgccag gtcccgtacc
     4501 agcactgtgc ccacaggtgt accgcctgag cgcggtagca ggcaaaattt tgctgccggt
     4561 cgggaaactg atcgatggtc gctggcaggt aaaggatctc gctgtcggta caggcttggt
     4621 cgccggtttc gatttgcagc cggcggccgt cgaggccgtg caggaagtgc tgcagcaagc
     4681 ggcgcacgct cgacagctcg accgcgcgcc agcgtttgtg gcgtgcggcg atgaacttgt
     4741 cgaaatcctg caacgcgcga atcgctggct cgagaccttg ctcgaagtag acgtcgaacg
     4801 ccgcctgcag ccagtgctcg cagtctttgc ggccgacgcg ttgcagcacc tcgccggcca
     4861 gccgcgcgaa ctggtagccg atttccggct cggtacgggc taccgacagc acccagcggc
     4921 tgacgaagcc tcgccagcga ggttcgagcg cctgcagccg ggcgacgatt tcgtcgaccg
     4981 tcgaggtgtt gaaataatgg gcctcgagct gtgcgcgcag ccgcgcttcg tcgtcactca
     5041 tgtctggcat accgggcagt agtaactcga gcgctgcgcc tgcacgatat gacgaatcgg
     5101 ctcgccgcat cgctggcact gcgcggtatt gccgtaaacg cgcaattcgt ggcgaaaata
     5161 cccgggccgc ccgtccgcct gcacgaagtc ctgcagtgtc gtgccgccgt gttcgatcgc
     5221 ttccgccagg gtcgcgcgga tcgcctcgac caggccggca atgcgcgcct tgctgatccg
     5281 ctgcgctgcg cgggtcggcc gaataccggc gcggtacagg gcctcgctgg catagatgtt
     5341 accgacgccg accacgatat ggctgttcat cagcaacccc ttgatcgcca cgcgccgtcc
     5401 gcggcagcga cggtacaggt agtcggcgtc gaattccgcg gacagcggtt ccgggccgag
     5461 gtcgcgcagc agccgatggg cgagcggatc gccggcgcaa tacagcagcg cgccgaatcg
     5521 gcgcggatcg cgaaacttca gcgactggcg attggccagc tgcaattcga cgtgctcgtg
     5581 atcctcgccg gggctgccgg tggcgagtac gcgcatgctg cccgacatgc ccaggtgcca
     5641 gatcaggttg ccgcgctgca gctgcatgat caggtacttg ccgcgccgcc gaagatcgac
     5701 gatgcgctgc ccgccgaggg tcggcacctc gcgactgacc ggccagcgca ggcgcgattc
     5761 acgcacgatc acccgctcga tgatctgccc tctgacatga ggctcgatgc cgcggcgggt
     5821 ggtttccact tcgggtaatt ccggcatgac tgcagcttaa tcctcgtgga atacccaggc
     5881 cagcgcgatg ccgcccatcc aggtggcggt ctcggagacc agcggactgt cttcggcctg
     5941 cgagccgcgc aggctgtcgt agcgcacgaa gccgccgagc cagtaaccgc cgatacggcg
     6001 gctgtaggtg aactcggtgc ggtagccgct gaagccgccc tcggcgctaa atgccgggcg
     6061 cgtagcggtc gcgtcggtgg tcgacaccga gtaaaaatag tcgtggtatt cgtcgctgga
     6121 gtaaagcggg ccgaagttga ggcgcagttt ccattcgccg agacgtgtcg ccggccgccg
     6181 ccacgacagc cgcggctgga atacgtagcc gacatgctcg aagtcggcat tcagcgtgta
     6241 cgccaggcgc aacggcagat ccagccacag gtcgctgtgc cgtagatgca ggaaccgcca
     6301 gttcagcgag ggtccgattt cgaccagtgc ctcgagctta tccatgccgt cgcgttccgg
     6361 ggtgtcctcg tcggcgggta gcgacaggtt gccgctcagt gtaagcagca ggtcgggggt
     6421 ttcgaaaatg atgccatcgg cgccctcgtc gacgcgcagg cggtggccgc ggtacttgac
     6481 gtagggtacc ggcagcaggt agttcgagga ctccgccgat ccgcggtagt cgggggcatt
     6541 gagcgcgatc aggccgatgc ccagttcgag gcgcggcagg ggctgctgcg cgcgcggccc
     6601 ggaacaggcc agcagcaaca ccaggctaag caggattctc atcgccgagc ggggtaaaca
     6661 ggcgatcgaa atactcgccg cgaagatggt attgcaggcc gagcgcggga ttggcgatga
     6721 tgatctcggg atcggtcgcc agcagcagca actcgatgtg ctcgccatcg ctgagcccgg
     6781 cgacgatgcg accggtcggt gcgcgcgggt cctggtagct gccgatgcgg gtcgcttcga
     6841 ggtgctgcca gtcggcgacc agcgcgacca gtgtttcggc gttcagatcc ccgcgttcgc
     6901 cagcctgcca ggcaccgccg gcatcgcggc gtagttcgag ccccggcagg ctcaatgtcg
     6961 tgatctcgaa atccggcggt aacaggcgct ggtcgatgaa cccggaaatg ccctgcagga
     7021 tcagcggcaa atgcacgtcc ggcaacaggt agagcgccga atcgatcagc gtgtaacggc
     7081 gttcgccgat attgttgaca ctgccgaaac ggatcgagag ctggccgagc tgcaattgcg
     7141 ccggcggttc gtcgagaccg aagcgggcta gatcgatagc ggcctcctgc agtggctcgg
     7201 ctgcgccggt atcgacgatt cgaaacagcc gctcgacatt ggcgcgatcg gcgggccagc
     7261 gcagcggtgc ggccagccgc cagcctgacg catgtttctg cagcgccagt tgctggtccg
     7321 ctgttcgcac ctcgatggcc tcgacggcag cggtatcgaa gccggcgtgc gcgggctgcg
     7381 atggcggctc gggctcgacc tcgaggcggt aaccgagggt tgccagcacc gcgatgacca
     7441 ggatcagcag gccgttgacg agccagcggc gcgacagcat cagagcttgc gccgcttgag
     7501 ccagatgcgc aggccgccgc cgagcagcga cagcggcagc aggatcagga agaagatcgc
     7561 gatccccagt tgctgcgctg ccgacagctc gagtcgcgta tcgggcgccg gccgcgggct
     7621 gatcgagatc agcccgtcat cgtggctgag ccagttgacc aggttgacgc cgatttccag
     7681 gttgctgccg ttgccaaggt attgattggc gaggaaatcg gcgtcgccga tgaccgccac
     7741 ccgctgcatc tgggcctgct cgtcgtcgtc gcgctggcgc gtgagagtca gcgcgagatc
     7801 cagcggtc
//
LOCUS       DATCZV010000021         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_583831, whole genome shotgun sequence.
ACCESSION   DATCZV010000021 DATCZV010000000
VERSION     DATCZV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583831"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VIW27_00460"
     CDS             <1..537
                     /locus_tag="VIW27_00460"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY5738158.1"
                     /translation="SKIYTRTGDDGTTGLADGSRVGKDSPRVEAMGAVDELNSQVGWV
                     LTHDLPDAVRECLIDVQHSLFDVGGELAIPGTAAITADRVSALERALDEFNADMPPLK
                     EFILPGGSTGAVSAHVARSVCRRAERRLLTLSCVEQVNEHSRKFLNRLSDLLFVIARL
                     LARASGGEVYWDSARNRK"
     gene            663..1187
                     /locus_tag="VIW27_00465"
     CDS             663..1187
                     /locus_tag="VIW27_00465"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY5738159.1"
                     /translation="MSSKKTWIIVGLVLAAAMLRIIPHPPNFAPVAAMALFAGAHLAD
                     KRIAFLLPLVAMGLSDIFIGFHDGIVLVYVCMCIAVAIGMQLRGKIGALSVAGGALLS
                     AVVFFVVTNFGVWLTSGMYPLTAGGLVACYVAAIPFFQYTVAGNLFFAAMLFGGYALI
                     ETRVPSLRGAVNSA"
     gene            complement(1263..1925)
                     /locus_tag="VIW27_00470"
     CDS             complement(1263..1925)
                     /locus_tag="VIW27_00470"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY5738160.1"
                     /translation="MAGTFRNVVISAIAAAAVAAASPVFAVEKISVKGLFNNKAIVVI
                     DGKQRLLRAGETSPEGVKLISANSKQAVLEIDGVQSTRGLGTHIGSTFTPAESTAVRI
                     WPTARNMYTIVGSINGLTVNFLVDTGATSIAMNSGEARRLGIDYAVEGEPTVSQTASG
                     LAKAWIINLKRVKVGDIELANVRAAVLEGNFPTEVLLGMSFLGRLDMRREQGVLELRK
                     KY"
     gene            complement(2002..2907)
                     /gene="gluQRS"
                     /locus_tag="VIW27_00475"
     CDS             complement(2002..2907)
                     /gene="gluQRS"
                     /locus_tag="VIW27_00475"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY5738161.1"
                     /translation="MTGSARSYRGRFAPSPTGPLHFGSLVAALGSYLDARSCGGEWLV
                     RMEDVDIPRSVPGAADSILRDLERFGLTWDGEVLVQSRRDAVYADAIANLRRLDLVYD
                     CGCSRRDLAGGVYPGTCRNGLAPGRSARSVRLRTDDSAVSFDDRVQGLFAQRLESDVG
                     DFVVYRADKLFAYHLAVTADDALQQITDIVRGADLLDSTPRQIHLQRCLELPTPRYAH
                     LPVAVNRQGWKLSKQTFAEPVSTAPVAPLLLQALEFLGQAPEDALRRAGAGEILDWGK
                     KHWRLENVPREREIYWPGPAFGTPA"
BASE COUNT          526 a          913 c          949 g          583 t
ORIGIN      
        1 tcgaaaattt atacccgcac cggcgatgac ggcaccacgg gtctggctga tggttcgcgg
       61 gttggcaagg acagtccacg cgtcgaggct atgggcgccg tggatgaact caacagccag
      121 gttggctggg tgttgaccca tgatctcccc gacgccgttc gggagtgtct gatcgacgtg
      181 cagcacagcc tgttcgacgt cggcggcgag cttgcgattc cgggcaccgc cgcgatcacc
      241 gccgaccgcg tatcggccct ggaacgcgcg ctggatgaat tcaacgcaga catgccgccg
      301 ctgaaggaat tcatactgcc cggcggcagc accggcgcgg tgtcggccca tgtcgcccgt
      361 tcggtttgcc gcagggccga gcggcggctg ctgaccttgt cctgcgtgga gcaggtcaac
      421 gagcacagtc gaaagtttct caaccgcttg tcggacctgc ttttcgtgat tgcccgcctg
      481 ctggcccgcg cgtccggcgg cgaggtgtac tgggattccg cgcgcaaccg gaaatgactt
      541 gcgcgcgtta tcattcaggg cggcgtggcg atagcccgtg cgccgcggcg tgcgttacaa
      601 tgccggccgg gtttctcatc acggcttggt cgcgataacc atcgatgcag cagaggttca
      661 aaatgtcgag caagaaaacc tggattattg tcggactggt gctggccgcg gccatgcttc
      721 gaatcattcc gcacccgccg aacttcgcgc cggtggccgc catggcgctg ttcgccgggg
      781 cccacctggc ggacaaacgt attgcttttc tgctgccgct cgtcgccatg gggctcagcg
      841 atatctttat cggtttccac gacggcatcg tgctggtcta cgtatgcatg tgcattgcgg
      901 tggccatcgg tatgcagttg cgcggaaaga tcggcgcact cagcgttgcc ggtggcgcgc
      961 tgctcagcgc ggtcgtgttt ttcgtggtaa cgaactttgg cgtctggttg acgtcgggca
     1021 tgtatccgct tactgcgggc ggactggtgg cgtgttacgt ggcggcaatt ccgtttttcc
     1081 agtacacggt tgccggcaac ctgttctttg cggccatgct cttcggcggc tacgcgctga
     1141 tcgaaacacg cgtaccgtcc cttcgcggcg cggtgaacag cgcctgaacc gagcagattt
     1201 ttacaaacgg ataaaaacgg cccgcaatgc gggccgtttt ttttggcacg cgtttatgtt
     1261 catcagtact tcttgcgcag ctcgagcacg ccctgctcgc ggcgcatgtc gagccggccc
     1321 aggaatgaca tgccgagcaa cacctcggtg ggaaaattgc cttccagcac ggcggcacgc
     1381 acgttggcca gttcgatatc gccgaccttc acccgtttca ggttgatgat ccacgccttg
     1441 gcgaggcccg aagcggtctg ggagacggta ggttcgcctt ccactgcgta gtcgatgccc
     1501 agcctccgcg cctcgccgga gttcatggca atgctggtcg ccccggtgtc gaccagaaaa
     1561 ttcaccgtca gtccgttgat gctgccgacg atggtgtaca tgttgcgtgc cgtcggccag
     1621 atgcgcacgg cggtgctttc agccggagtg aaggttgacc cgatatgcgt gccgagaccg
     1681 cgggtgcttt gaactccgtc aatttccaga accgcttgct tgctgttggc cgatatcagc
     1741 ttgacgcctt ccgggctggt ttcgccggcc cggagcaatc gctgtttgcc gtcgatgacg
     1801 acgattgcct tgttattgaa cagacctttg acgctgattt tctccaccgc gaaaaccggc
     1861 gaggcggcag ccacggcggc ggcagcgatc gcggaaataa cgacgttgcg aaaggttcca
     1921 gccatcggag tcagcggtgc atcaaggtcg ccatctcagg aaagtctaga aggattctac
     1981 cggccgcgcg gggcggcctc atcacgccgg ggtcccgaag gcagggcctg gccagtagat
     2041 ctcgcgttcg cggggcacgt tttccaggcg ccaatgcttt tttccccagt cgagaatctc
     2101 gcccgcgccg gcacgtcgaa gcgcatcctc cggggcctgg ccgagaaact cgagggcttg
     2161 cagcagcagc ggcgcaacgg gcgcggtgga gaccggttcg gcgaaagtct gcttgctcag
     2221 tttccacccc tgacggttga ccgccaccgg caagtgggcg taccgcggcg tcggcagttc
     2281 gaggcaacgc tggagatgga tctgccgcgg cgtcgaatcg agcaaatccg cgccgcgcac
     2341 gatatcggtg atttgttgca gggcgtcgtc ggccgtcacc gccaggtggt aggcaaaaag
     2401 tttgtcggcg cgatagacga cgaaatctcc gacgtcggat tccaggcgct gggcgaacag
     2461 accttggacg cgatcgtcga acgacaccgc gctgtcatcc gtgcgcaggc gaacgctgcg
     2521 tgcgctgcgc ccgggcgcaa gtccgtttcg gcaagtgccc ggataaacac cgccggcaag
     2581 atcgcgccgg ctgcagccgc aatcgtagac cagatccagg cggcgcaaat tggcgatcgc
     2641 gtcggcgtaa acagcgtcac gccggctttg caccaggacc tcgccgtccc aggtcagacc
     2701 gaatcgttcg aggtcgcgca agatggaatc cgccgcgccg ggaacgctgc gcggaatatc
     2761 cacgtcttcc atgcgcacca gccactcgcc gccgcagctt ctcgcatcga gatagctgcc
     2821 aagggcggcg accagcgaac cgaaatgcaa tggcccggta ggcgagggcg caaaccgccc
     2881 gcgatacgac cgagcgctgc cggtcatttc aggcgtctag cgatacaacg ttggcgggga
     2941 tttcggccgc aaatacacgg cccgaaaatc g
//
LOCUS       DATCZV010000022         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_1769, whole genome shotgun sequence.
ACCESSION   DATCZV010000022 DATCZV010000000
VERSION     DATCZV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1543)
                     /locus_tag="VIW27_00480"
     CDS             complement(23..1543)
                     /locus_tag="VIW27_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEY5738162.1"
                     /translation="MDVHHPVFWVAAGLVLLFVIGVLMAPEAAKGAFDGAKWWSIGAF
                     DWLFMLGGNIFVVFCLALIFLPVGKIRLGGQDASPEFSTLSWFSMLFAAGMGIGLMFW
                     SVAEPTAYYTGWYETPLGVEANTAAAAEKAMSATMFHWGLHPWAIYAVVALSLAFFHY
                     NKGLPLTIRSAFYPLIKDRSWGFFGHVIDTVAVLATLFGLATSLGFGAQQASAGLSYL
                     FGTDAGIGSQIAIIIIVTAIALVSVVRGLDGGVKVLSNINMSIAILLLLFVLAVGPTL
                     LIFKTMGTTVSGYLGDIFSYSNWVDREDEAWMQGWTVFYWAWWISWSPFVGMFIARVS
                     KGRTVREFIIAVLLVPTAVTVVWMSVFGGSALDQIQNNVGALANGLDKVELAMFQMLE
                     NLPLTAITSFVAIVLVLVFFITSSDSGSLVIDAITAGGKVDAPVVQRIFWVMIEGVVA
                     AALLFGGGADALTALQAAAITVGLPFTVVLIVMCFSLYLGLAHEKAYVIEAESPPA"
     gene            complement(1767..>2065)
                     /locus_tag="VIW27_00485"
     CDS             complement(1767..>2065)
                     /locus_tag="VIW27_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY5738163.1"
                     /translation="PTGAKGLNLAASDVYYLWQALLRHYRDGDDEGLERYSNSALARV
                     WKAERFSWSMTRLLHRFADQSAFEQRMQQAELAYLESSSAAQASLAENYIGLPY"
BASE COUNT          438 a          672 c          632 g          323 t
ORIGIN      
        1 ctccgcgatc ctgacgccgg gcttaggccg gcggcgactc ggcctcgatg acgtaagcct
       61 tctcgtgtgc caggccgagg taaaggctaa agcacatgac gatcagcacc accgtgaacg
      121 gcaggccgac agtgatcgcc gccgcctgca gtgcggtcag cgcatccgcg ccgccgccga
      181 acagcagggc cgcggccacc acgccctcga tcatcaccca gaagatacgc tgcaccaccg
      241 gcgcgtcgac cttgccgccg gcggtgatcg cgtcgattac cagtgagccg gagtcggacg
      301 aggtgatgaa gaacaccagt accagcacga tggcgacgaa cgaggtgatc gcggtcagcg
      361 gcaggttttc gagcatctgg aacatcgcca gctcgacctt gtcgagaccg ttggccagcg
      421 cgccgacgtt gttctggatc tggtccagcg ccgaaccgcc gaagacgctc atccacacca
      481 cggtaactgc ggtgggcacc agcagcactg caatgatgaa ttcgcgcacc gtgcgcccct
      541 tggaaacgcg ggcgatgaac atgccgacga acggcgacca ggaaatccac cacgcccagt
      601 agaacacggt ccagccctgc atccaggcct cgtcctcgcg gtcgacccag ttgctgtagg
      661 aaaaaatatc gccgaggtaa ccggagaccg tggtgcccat ggtcttgaat atcagcaagg
      721 tcggaccgac cgccagcacg aacaatagca gcaatatcgc gatactcatg ttgatgttgg
      781 acagcacctt gacgccgccg tcgagcccgc gaacgaccga caccagcgcg atcgcggtga
      841 cgataatgat gatcgcgatc tgcgagccga taccggcgtc ggtgccgaac aggtaggaaa
      901 gtcccgcgct cgcctgctgc gcgccgaagc ccagcgaggt ggccaggccg aacagggtcg
      961 caagcaccgc cacggtatcg atcacatggc cgaaaaagcc ccaggaacga tccttgatga
     1021 gcgggtagaa cgccgagcga atcgtcagtg gcaagccctt gttatagtgg aagaacgcca
     1081 gcgacagcgc gaccaccgcg tagatcgccc acggatgcag gccccaatgg aacatggtcg
     1141 cgctcatcgc cttttcggcg gctgccgcgg tgttggcctc gacaccgagc ggggtttcgt
     1201 accagccggt gtaataagcg gtcggctcgg cgacgctcca gaacatcagg ccgatgccca
     1261 tgcccgcggc gaacagcatc gagaaccacg acagggtgct gaattctggc gaggcgtcct
     1321 ggccgcccag cctgatcttg cccaccggca ggaagatcag cgccaggcaa aagacgacga
     1381 agatattacc gcccagcatg aacaaccagt caaaggcgcc gatcgaccac cacttggcgc
     1441 cgtcgaaggc gcccttggcg gcttccggcg ccatcagcac gccgatcacg aacagcagta
     1501 ccagcccggc ggcaacccag aataccgggt ggtgcacatc catgccccag cgctgcaggt
     1561 tatcctgccc ggcgacgtaa tcggtttcgt aaagtgtgct cttatcagtc acgtcccccc
     1621 cctctcgatg actcgagttt attatcgttt caggtatcaa gctcccggga ggataacggc
     1681 tatgacctgt cctgtctaac cggctacagg gagcggccgg cgaatcgcgg ccaggctgac
     1741 tgccgaaggc gggccagcgc agcgaatcag tacggcaggc cgatgtaatt ctcggccagc
     1801 gaggcctgtg ccgcgctgga ggattcgaga taggcgagtt ccgcctgctg catgcgctgc
     1861 tcgaacgccg actgatcggc gaagcggtgc agcagccgcg tcatgctcca gctgaagcgc
     1921 tcggccttcc agaccctggc cagcgcgcta ttcgagtaac gttcgaggcc ttcgtcgtcg
     1981 ccgtcacggt agtggcgcag cagcgcctgc cacaggtagt agacgtccga agccgcgaga
     2041 ttgagaccct tggctcccgt cggcg
//
LOCUS       DATCZV010000023         4873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_160657, whole genome shotgun sequence.
ACCESSION   DATCZV010000023 DATCZV010000000
VERSION     DATCZV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160657"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1082)
                     /locus_tag="VIW27_00490"
     CDS             complement(138..1082)
                     /locus_tag="VIW27_00490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738164.1"
                     /translation="MPATRSVNKQLSAAVVAAEKRLRHTRYGLLAPGILTIFVISIMP
                     LSIVLVYSFLKPGDFGGVTWEFSTQAYLQLLFEEDIFDETLSFTTDYLEIFFRSVSLG
                     VFTTIACLVVGFPTAYFIATQPPSRRNAWLFLITIPFWTNLLIRTYAMLLILRDQGVI
                     NLSLIKIGVIDRALPLLYTDFAVGLGLVYAYLPFMVLPIFASLEKLDFRLIEAGYDLY
                     ATRFKVLKRIIIPLAKPGIAAGCILVLIPSLGAFITPALLGGGKSLMIGNLINLQFGQ
                     ARNWPFGSAIALVLMVLVMLSLLAYLRYGARKGGSSHG"
     gene            complement(1086..2192)
                     /locus_tag="VIW27_00495"
     CDS             complement(1086..2192)
                     /locus_tag="VIW27_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5738165.1"
                     /translation="MAEQSSTVAIDIRATSKIYGRDRETGVVALDEVSLAIRDNEFFT
                     LLGPSGCGKTTLLRLIAGFEQPTQGEILLFGEHIEGLPPYKRPVNTVFQHYALFPHMS
                     VAQNIGFGLEMLDIASSQVDKTVQEVLALVQMEHLAERKTDQLSGGQQQRVALARALA
                     PRPKVLLLDEPLSALDFKLRKEMQVELKRLQHETGITFIFVTHDQEEALTMSDRIAVM
                     RDGLILQVGSPGEIYDRPARRFVADFIGDINILSGRLLQRQGDRARIRLAAGYEGDAG
                     LPADSGGLGDEIHVAIRPEQLSFVDDPSGAHLAGSIRQVSYFGSGFDFEIELADGETI
                     TLQEQNRGAGHRRREVGDSVQVAISDNSMQILAD"
     gene            complement(2435..3235)
                     /locus_tag="VIW27_00500"
     CDS             complement(2435..3235)
                     /locus_tag="VIW27_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5738166.1"
                     /translation="MHNKTIAPGRERRRTDSLSRAIDAIGHSDFLPNLLDYLCVDVPF
                     VGILLLLLDEERRPLHIYDTIRTPYRINLDMYLDGVYQLDPFYTRFSKQRKTEAMLIR
                     DVAPDRFNQTEYYRRFYKNMELQDEMAIFTDLQNGCFLFFSIGRRATEMRFRRRELRA
                     VERDLPVLAALCRRHFGDSSYNRESLAGNVGDERLRFALERFGGDALTPREHEVAVCI
                     LKGHSSKSLAREFDISPETVKIHRRNIYRKLGISSQSELFLRFLNTLG"
     gene            complement(3472..3999)
                     /locus_tag="VIW27_00505"
     CDS             complement(3472..3999)
                     /locus_tag="VIW27_00505"
                     /inference="COORDINATES: protein motif:HMM:NF022115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HEY5738167.1"
                     /translation="MTITPQIPTFAAAVYKPGTADRQALSRFVAQLLARNTRVAGILQ
                     ETIVDSSGDVIGLNAVDISTDSRLPISRPTKNDDECGLDVSALVETAGIIRKAISERA
                     ELVVVEKFGEQEQGGKGLNDEILQAIAADIPLLIAVPEAALPIWQERTGELGSVLPFD
                     EEAFRRWWQRITGGH"
     gene            4429..>4873
                     /locus_tag="VIW27_00510"
     CDS             4429..>4873
                     /locus_tag="VIW27_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY5738168.1"
                     /translation="MSRSPFVAPFEELPNSLPIFPLSGAVLMPHAELPLNIFEPRYLN
                     MVNDAIGSHRMIGMIQPDAKAGSDEAIYPVGCAGRITQFRETRDGRYEMVLSSVCRFE
                     ASEELPTIRGYRLVTPDWSRFACDYDEPDSGLQAEQPRLMTTLERY"
BASE COUNT          974 a         1395 c         1530 g          974 t
ORIGIN      
        1 cggtcaccga gcgattctgg ttgaaggaga acagcaccag taccgctatc ggcgcataca
       61 ggacgatgat gcaaaaccag gcgatttgcg agaaaccggg ctggtacttg agatccatgc
      121 ccttgccgct ggcggtctca gccatgcgag gaccctccct tgcgtgcgcc gtaacgcagg
      181 taggcgagca gcgacagcat caccagcacc atcaatacca gcgcgatcgc cgaaccgaat
      241 ggccagttgc gtgcctggcc gaactgcagg ttgatcaggt tgccgatcat caacgatttg
      301 ccgccgccga gcagcgccgg ggtgatgaag gcgccgaggc tggggatcag cacgaggata
      361 cagccggccg cgattccggg cttggccagc ggaatgataa tgcgcttcag caccttgaag
      421 cgcgtcgcgt agagatcgta accggcctcg atcaggcgaa aatcgagctt ttcgagactg
      481 gcgaagatcg gtaataccat gaacggcagg taggcgtaga ccagccccag cccgaccgcg
      541 aaatcggtgt acagcagcgg cagcgcgcga tcgatcacgc cgatttttat cagcgacagg
      601 ttgatcacgc cctggtcgcg caggatcagc agcatcgcat aggtgcggat cagcaggttg
      661 gtccagaacg ggatcgtgat caggaacagc cacgcgttgc gccgcgatgg cggctgggtg
      721 gcgatgaagt aggcggtcgg aaagccgacc acgaggcagg cgatcgtggt aaaaacgccg
      781 aggctgaccg agcgaaagaa gatttccagg tagtcggtgg taaacgacag cgtttcgtcg
      841 aagatgtcct cttcgaacag cagctgcagg taggcctggg tcgaaaattc ccaggtgacg
      901 ccgccgaagt cgcccggttt cagaaacgag taaaccagca cgatcgacag aggcatgatg
      961 ctgatgacga atattgtcag gatgccgggc gcgagcagcc cgtagcgcgt atggcgcagg
     1021 cgtttttccg cggcgacgac cgcggcggac agctgtttgt tgacggatct ggtcgcgggc
     1081 atggtctagt ccgccaggat ctgcatgctg ttgtcgctga tcgcgacctg cacgctgtcg
     1141 ccgacctcgc gccggcgatg cccggcaccg cggttttgct cctgcagcgt gatggtttca
     1201 ccgtccgcca gctcgatctc gaaatcgaaa cccgagccga agtaagacac ctgtcgaatt
     1261 gagccagcca ggtgagcgcc gctcggatcg tcgacgaaac tcagctgctc gggacggatt
     1321 gcgacgtgga tttcatcgcc gaggccgccg ctatcggcag gcagaccggc gtcgccctcg
     1381 taaccggcgg caagcctgat tcgggcgcgg tcgccttgcc gctgcagcag ccggccgctc
     1441 aggatattga tatcgccgat aaaatcggcg acgaagcgcc gcgccggccg gtcatagatt
     1501 tctccgggac tgccgacctg caggatcagg ccgtcgcgca tcacggcgat gcgatccgac
     1561 atggtcagcg cctcttcctg gtcgtgggtc acgaagatga aggtaatccc ggtctcgtgc
     1621 tgcaggcgct tgagttcgac ctgcatttcc ttgcgcagct tgaagtcgag cgcggacagc
     1681 ggctcgtcga gcagcagcac cttgggccgc ggcgccagcg ctcgcgccag tgcgacgcgc
     1741 tgttgctggc cgcctgagag ctggtcggtc ttgcgctcgg cgagatgttc catctgcacc
     1801 agcgcgagca cttcttgcac cgttttatcg acttgcgatg acgcgatgtc gagcatttcc
     1861 aggccgaaac cgatattttg cgcgacgctc atgtgcggga acagcgcgta gtgctggaac
     1921 acggtattga ccggccgttt gtatggcggc aggccctcga tgtgttcgcc gaacagcagg
     1981 atttccccct gcgtgggctg ctcgaagccg gcgatcaggc gcagcagcgt ggtcttgccg
     2041 catcccgagg gaccgagcag ggtgaaaaac tcgttgtcgc ggatcgccag cgacacttcg
     2101 tccagcgcga ccacgccggt ttcacggtcg cggccgtaaa tcttgctggt tgcgcgaata
     2161 tcgatggcaa cggtcgacga ttgctccgcc acgctgtagc ccccgggttg tcgttattat
     2221 ggccgacagc ttagtttcag cgttgcggaa ctgtatataa cctaaaaggg ttaacctcgc
     2281 cgcaatccgc gaagccgttt cgcagccgat gttcgcagag gcgggtgacc cgcaaatctc
     2341 gagcagcgcg tatctttaca ggattgccgc tcgcgcggga gtgacaggag acacgcgcgg
     2401 ataactgcag gggctgcgac aggtcgcggg atcactagcc cagcgtgttc aggaaacgca
     2461 gaaacagctc gctttgcgac gaaatgccga gcttgcggta gatattgcgg cgatggattt
     2521 tcacggtttc ggggctgatg tcgaattcgc gcgcgagcga cttcgacgaa tgccccttga
     2581 ggatgcaaac cgcgacctcg tgctcgcgcg gcgtcagcgc atcgccgccg aaacgttcca
     2641 gcgcgaaccg cagacgctcg tcgccgacgt tgccggcaag gctttcgcgg ttgtacgagg
     2701 aatcgccgaa gtgccgccgg cacagcgccg cgagcaccgg taaatcgcgc tcgacggcac
     2761 gcagttccct gcgcctgaag cgcatttcgg tggcgcgccg gccgatcgaa aagaacagga
     2821 agcagccgtt ctgcaggtcg gtgaaaatgg ccatctcgtc ctgcagctcc atgttcttgt
     2881 agaaacgccg atagtactcg gtctggttga aacggtccgg ggcaacgtcg cgaatcagca
     2941 tcgcctcggt tttgcgctgt ttgctgaatc gggtgtaaaa cggatccagc tggtagacgc
     3001 cgtcgagata catatcgaga ttgatccggt aaggtgtgcg gatggtgtcg taaatgtgca
     3061 acgggcgccg ctcctcgtcg agcagcagta gcaggatacc gacgaagggc acgtcgacgc
     3121 acaggtaatc gagcaggttg ggcagaaaat cgctatggcc gatcgcgtca atcgccctgg
     3181 acagggagtc ggtacgccgc cgttcgcggc cgggcgcgat ggttttgttg tgcacgaatc
     3241 gagatcgccg ccggtctgat caactctcga tgttacagca tgcccgggca aaaatcgaaa
     3301 ataggtgtca gagagcaata gtttttaata ttgttggaaa ttgctctttg gcacctatta
     3361 tttgcggcag tcacgccggg atcaacccga gccgcatcgt gccgcgcaac ccgaagatcg
     3421 acgcgacgcg cacttgcctg gcggccgcga acggtgctcc gcgagccagg tttagtggcc
     3481 cccggttatc cgctgccacc accgccgaaa ggcttcctcg tcgaagggca gaacgctgcc
     3541 gagttcgccg gtgcgttcct gccagatggg taatgccgcc tcgggaacgg cgatcagcag
     3601 cggtatgtcc gcggctatgg cttgcaggat ctcgtcgttc aaacccttgc cgccttgctc
     3661 ctgttcgccg aatttttcaa ccacgacgag ttccgcgcgc tcgctgatgg ctttgcggat
     3721 aatacccgcc gtctccacca gggccgatac atccaggccg cactcgtcgt cattcttcgt
     3781 cggccggctg atggggaggc ggctgtcggt cgaaatatcg acggcattca gtccgattac
     3841 gtcgccactg gagtcgacga tcgtctcctg caaaatcccg gcaacccgcg tgttgcgggc
     3901 taacagttgt gcgacgaacc gggacagcgc ctgcctgtcg gccgtgcccg gtttgtaaac
     3961 tgccgccgcg aaggtgggga tttgtggagt aatcgtcatc ggatagcgga caggcgctgt
     4021 tgcgcaataa tcgaggctaa caggttacag ttgttttgta tcaatggctt gatcccgatc
     4081 aagcgaaatc ccgttgccaa aagtggagtg ccgggggcag tatattgaat ttcgagtcac
     4141 agtgtcagtg ccggataagc gggcggggtc gggcctcaca tttttcaacg agagcaaaga
     4201 tcggatctga aatgcaaggc ctgccccaat cttttagtct tctttcgcat tcaaccgctc
     4261 cacgctcggc ggaatttctg aagtttcttt tccaggtcat gctggaaggt gatcggtgaa
     4321 ggactcgttg ccgtaagaaa tgaagcgggt tgtcgaatca gtctgctacg ctgttgccca
     4381 ggctgcgtgc tgatatggac atggcgctaa atctaagagg attaaggtat gagtagaagc
     4441 cctttcgttg cgcctttcga ggagctaccg aacagtttgc cgatttttcc cctgtcgggc
     4501 gcggtgctga tgccgcatgc cgaactgccg ctgaacatct tcgagccgcg ttatctcaac
     4561 atggtgaacg atgccatcgg cagccatcgc atgatcggca tgatccagcc cgacgcgaaa
     4621 gccggcagcg acgaggcgat ctacccggtg ggctgtgccg gtcgcatcac ccagtttcgc
     4681 gaaacccgtg acggtcgtta cgagatggtg ttgagcagtg tctgtcgctt cgaggccagc
     4741 gaagaattgc cgactatccg cggttatcga ttggtgacgc cggattggtc gcgtttcgcc
     4801 tgtgattacg atgagcccga ttcaggcctg caggccgagc agccgcgctt gatgaccact
     4861 ctcgagcgct atc
//
LOCUS       DATCZV010000024         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_425372, whole genome shotgun sequence.
ACCESSION   DATCZV010000024 DATCZV010000000
VERSION     DATCZV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425372"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VIW27_00515"
     CDS             <1..560
                     /locus_tag="VIW27_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY5738169.1"
                     /translation="AAHHLNKLDSPPAIVFTTAYDEYAIDAFDARAIGYVLKPVRRER
                     LASALQQAARLAAGTLADVAAASHMESRRKHVCSASHGQLKLIPVDDVRYFRADQKYV
                     CVHHVGGQDLIDDSLKSLEEEFADEFVRIHRGAIVAVDKIERLAKTSDGKHHVVLRDG
                     SQEPENELIISRRHLADVRRRLKGG"
     gene            567..1493
                     /gene="hemC"
                     /locus_tag="VIW27_00520"
     CDS             567..1493
                     /gene="hemC"
                     /locus_tag="VIW27_00520"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5738170.1"
                     /translation="MLIRIATRKSALALWQAEHVAERLQQLDEVNSVELVPLSTRGDE
                     ILDRSLQKIGGKGLFIKELEVAMQQGDADIAVHSMKDVPAEMPEGFCVAAVLDRASHA
                     DAFVSTHYDSLDALPEAARVGSSSLRRQAQLRMMRPDLVIEPLRGNVNTRLAKLEAGE
                     YDAIILAAAGLERLGLQQNIRQQFETEQMLPAAAQGVIGIECLAYRADLRGVLAKLEH
                     AETARTTAAERAVALTLQANCQSPVAAFATIAGDRLRLTALVALPDGSRSLRDSREGP
                     AGDAEQIGRQLAVSLLEQGAGELLEQAATADG"
     gene            1486..2265
                     /locus_tag="VIW27_00525"
     CDS             1486..2265
                     /locus_tag="VIW27_00525"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5738171.1"
                     /translation="MADTPLAGVGVLVTRPAGQAEELIAAIKAAGGRSIVFPVIEIAP
                     RSPQDIASDLAGLPDPDIVIFVSRNSVEHGLAWSEGGAIAAVGPATASAIEAAARVVD
                     IRPASGFDSESLLEEPALTDVRGKTIRIIRGNGGRELLATTLRERGANVDYLEVYARR
                     TPDYPEAEIADLVRQLEAGDIAVVIVMSVESFHNLLVLLPESCHEALAKTLLVTPAAR
                     VIKQVTGRLPGCPATLADGPGADEIVRAIANLAPHSSGQNS"
     gene            2262..>3131
                     /locus_tag="VIW27_00530"
     CDS             2262..>3131
                     /locus_tag="VIW27_00530"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY5738172.1"
                     /translation="MSEETKDKETAEAGTGEPVSAEAADEEPQADSPAPEEREVPVES
                     KEEPVETAEPEPRPPPAAKPARVTSPVAWLALFLALLTLAAVGYMIVEDRRTRAVEAA
                     GSQSLERLASRVDESRESLSGLDNSLDELASSDEALESRIAGLQGEVERRAEILESMP
                     GRMSNLENSVAALQGASAGARDTWLLAEAEYYMQIANAQLQLGNNPKLAMLALEMADE
                     RVVQMANPALTDVRRSLADELAALEAMEKPDLQGATLTLASLARVVETLPLRATGSTT
                     GETGGASAEELGAA"
BASE COUNT          610 a         1006 c         1040 g          475 t
ORIGIN      
        1 aggcggcgca tcacctgaac aagctggatt cgccgcccgc catcgtcttc accacggcct
       61 acgatgaata cgccatcgat gccttcgatg cgcgcgccat cggttatgtg ctgaagcccg
      121 tgaggcgcga acgcctcgcg agtgcgctgc agcaggcggc acgactcgcg gcgggcacgc
      181 ttgccgatgt ggcagccgca tcccacatgg agtcgcgcag aaaacatgtc tgctcggcgt
      241 ctcacggtca gctgaagctc atccccgtcg acgacgtgcg ttatttccgg gctgaccaga
      301 aatacgtctg cgtgcatcat gtcggcggcc aggacctcat cgacgattcg ttaaaatcgc
      361 tggaagagga gttcgccgac gaattcgtac gcattcatcg gggcgccatc gtagcggtgg
      421 acaagatcga acggctcgcg aagacctcgg acggcaaaca ccacgtcgtg ttgcgcgacg
      481 gctcgcaaga accggaaaac gagttgataa tcagccgtcg gcatctcgcc gacgtccgca
      541 ggcgcctgaa gggaggttga agaccattgt tgatccgaat cgcaacacgc aagagtgcgt
      601 tagcgctctg gcaagccgag catgtcgcag agcgactgca gcaactcgac gaagtcaaca
      661 gcgtggagct ggtgccactg tcgacgcgtg gtgacgaaat actggaccgc agcctgcaga
      721 agatcggcgg caagggcctg tttatcaagg aactcgaagt cgcgatgcag cagggcgatg
      781 ccgacatcgc cgtacactcg atgaaggacg tgccggctga aatgccggag ggattctgtg
      841 tggccgccgt gctggaccgt gccagccatg cggacgcttt cgtctccact cactacgact
      901 cactcgacgc gctgcctgaa gcggcgcggg tcggcagttc cagcctcagg cgccaggccc
      961 agttgcggat gatgcgtccc gacctggtca tcgaaccact gcgcggtaac gtgaatacgc
     1021 ggctcgcgaa gctcgaggca ggcgagtacg acgccatcat tctcgccgct gccggactcg
     1081 aacggctcgg cctgcagcag aacatccggc agcagtttga gactgaacag atgctgccgg
     1141 ctgccgcaca gggggtcatc ggcatcgagt gcctcgcgta tcgcgcggat ctgcggggcg
     1201 tgctggcaaa gctcgagcat gccgaaaccg ccaggacgac cgcggctgag cgcgcggtcg
     1261 cgctgaccct gcaggcgaac tgccagtcgc ctgtcgccgc cttcgccacc attgccggtg
     1321 accggttgcg gctgaccgcg ctcgtcgccc tgccggacgg cagccgctcg ctgcgggact
     1381 cgcgcgaggg tccggccggg gacgccgaac agatcggcag gcaacttgca gtatcgctgc
     1441 tcgaacaggg tgcaggcgaa ctgctcgaac aggcggccac cgcggatggc tgacacgccc
     1501 ctcgcgggcg tcggcgtact cgttacgcgc cctgccgggc aggccgagga actcatcgca
     1561 gcgatcaagg cggcaggagg ccgcagcatc gtcttcccgg ttatcgaaat cgcaccccgc
     1621 agcccgcagg acatagcttc cgaccttgcc gggctgccgg acccggacat cgtcatattt
     1681 gtgagccgca attccgtcga gcacggattg gcctggtcgg agggcggcgc gatcgccgcg
     1741 gtcggccctg caaccgcctc tgcgatcgag gccgcggccc gggtcgtaga catccgcccg
     1801 gcaagcggtt tcgacagcga gagcctgctc gaagaaccgg cgctgacaga cgtgcgcggc
     1861 aagacgatcc gcatcattcg cggcaacggt gggcgcgagc tgctggcaac gaccctgcgc
     1921 gaacgcggcg cgaacgtaga ctacctcgag gtctacgccc gccggacgcc tgactacccg
     1981 gaggcggaaa tcgcggacct cgtacggcag ctggaggcgg gggacatcgc ggtcgtaatc
     2041 gtcatgagcg tggagtcatt ccacaatctg ctggtattgc tgcccgaatc ctgccacgag
     2101 gcattagcaa aaacgctact ggttacgccg gcggctcgtg tgataaaaca ggttacaggc
     2161 cgcctccccg gttgtccggc gacacttgcg gacgggccgg gtgcagacga gatcgtgcgc
     2221 gcaatcgcga acctcgcgcc tcacagttcc ggacagaact catgagcgaa gaaacaaaag
     2281 acaaggaaac ggcggaagcc gggaccgggg aacccgtgtc ggccgaggcc gccgacgaag
     2341 agccgcaggc cgatagccca gcgcccgaag agagggaagt cccggtcgaa agcaaggaag
     2401 agcccgtcga aacagcagaa cccgagccgc ggccaccgcc tgccgcgaag cccgctcgcg
     2461 tcacatcgcc tgtcgcctgg ctggcgctgt tcctggcctt gctgacgctg gcagccgttg
     2521 gctacatgat cgtggaagac cgccgcaccc gcgccgtcga ggcggcaggc agccagtcgc
     2581 tcgagcgcct ggccagccgt gttgacgagt cccgcgagtc cctgtccggc ctggacaaca
     2641 gcctcgacga actcgcatcg agcgacgagg ccctggaatc gcgtatcgcc ggcttgcagg
     2701 gcgaagtcga gcgccgggcc gaaatcctcg agtcgatgcc cgggcgcatg tcgaacctcg
     2761 aaaattccgt tgccgcgtta cagggtgcct ccgcgggtgc acgggatacc tggttgctcg
     2821 cagaagcgga gtactacatg cagatcgcga atgcgcagct gcagctcggc aacaacccga
     2881 agctcgcgat gctcgcgctc gagatggccg acgaacgcgt cgtgcagatg gccaaccctg
     2941 cgctcaccga cgtgagacgc tcactggccg atgagctggc cgcgctcgaa gccatggaaa
     3001 agccggacct gcagggtgcg acgctgacgc ttgcaagcct ggcgcgggtg gtggagacgc
     3061 tgccactgcg ggcgacgggg agcactacgg gcgaaacggg gggcgcatcg gctgaagagc
     3121 tgggtgctgc c
//
LOCUS       DATCZV010000025         9268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_266385, whole genome shotgun sequence.
ACCESSION   DATCZV010000025 DATCZV010000000
VERSION     DATCZV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266385"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VIW27_00535"
     CDS             complement(<1..343)
                     /locus_tag="VIW27_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5738173.1"
                     /translation="MNSNRAFIDRFKAYYRELHRSDPSRLHALYADDVVYQAPSRKLR
                     GLVQLEDYHAALIDDAGVCRFEFLDEIVGENSAYLKWILHAGHSRSSDPRQGLRGVSH
                     LKWGERIHYHED"
     gene            complement(427..942)
                     /locus_tag="VIW27_00540"
     CDS             complement(427..942)
                     /locus_tag="VIW27_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY5738174.1"
                     /translation="MRIVAAALPVAWLAACGSPQPIATVQQVDLQRFMGDWYVIASIP
                     TVFERGAHNAIETYRLDDDGSVATRFSFRDGGFDGPREVYDARGFVEDRETNAVWQIR
                     FFWPFEAEYRIVYLTDDYTRAIIGRSQRDYAWIMAREPAIDERELADMLAFLSAQGYD
                     ISLVERVPQRW"
     gene            complement(956..1699)
                     /locus_tag="VIW27_00545"
     CDS             complement(956..1699)
                     /locus_tag="VIW27_00545"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HEY5738175.1"
                     /translation="MKRRIASVVAGLSLAAASGWLQAAPRADQELVFRVLLDDSEIGY
                     HRFHVMQREQQQVIDIDARFKVTFLGIPVYRYDHRNRETWRQGCLESIVSTTEDGGDD
                     FRVDGRDTGAQFEVSTLDGAAELDAGCVMSFAYWDRDFLRQNRLLNAQNGEYLAVEIT
                     AEGVEELQVNDRTVSASRHRLVNSEQEIDITVWHALEDGRWLSLESRVDGRVISYRLA
                     NREQVEALRSTMQVAAGDDGAPAKRESGR"
     gene            complement(1696..2502)
                     /locus_tag="VIW27_00550"
     CDS             complement(1696..2502)
                     /locus_tag="VIW27_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY5738176.1"
                     /translation="MFDLTAYLIALGVILTAGVGVWLVSLLKRDVSIVDSLWPLMFLF
                     AALTFYLTLTDTGPRAVLLLVLVAIWALRLFAYITWRNWGEEEDRRYQQIRANNQPNF
                     ELKSLLIVFVLQGVIAWIVALPLLIAIGGAAPLGALDYLGLALWGIGMLFEAGGDFQL
                     ARFKADPANRGKVMDRGLWRYTRHPNYFGNACIWWGFGLMAGAAGAWWALLSPVLMTF
                     LLLKVSGVSLLEQDIGERRPAYAEYIVRTSAFVPLPPKRSGVGDQSVSRS"
     gene            complement(2504..3058)
                     /locus_tag="VIW27_00555"
     CDS             complement(2504..3058)
                     /locus_tag="VIW27_00555"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HEY5738177.1"
                     /translation="MKLFINFVIFQICWIVCILGGANGMPLLGVGFVAAAVAYHLLRA
                     EDARAEGTLILAAALIGVVWESLLVAAGWLVYPSGTLVAGTAPYWIVALWVVFATTFN
                     LSMRWFKQRLLLAALFGAAGGPLAFYAGSRLGGVEFSDTTIALTALGLGWMVLMPSMM
                     LLAKRFNGYPETIPARAALAAGEG"
     gene            complement(3067..4146)
                     /locus_tag="VIW27_00560"
     CDS             complement(3067..4146)
                     /locus_tag="VIW27_00560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY5738178.1"
                     /translation="MRFPVGWSGIVSAAADRALGWVELGLVPDRVVRAGIRRLLAQRL
                     QEIDAEDIESAAVRAEEFVRMMDASPIAPLPEKANEQHYELPAEFFVEVLGPQRKYSS
                     CYWGPGEQTLERAESESLRITCERAGIADGMQILELGCGWGSLTLWMARKYPAARFTA
                     VSNSSGQRAYIEEVATREGLDNLRVITADMNDFDIDQQFDRVVSLEMFEHMRNYRRLF
                     ARVHDWLRPGGRFFMHIFCHRLIPYEFVERDASDWMSRHFFSGGIMPSDALPLKFQDD
                     LRLRQQWRWNGRHYEKTLNAWLAKMDARKPQLMPILESVYGDQAAVWWMRWRVFFMAC
                     AELFGYHEGREWWVSHYLFERPGND"
     gene            4326..4904
                     /locus_tag="VIW27_00565"
     CDS             4326..4904
                     /locus_tag="VIW27_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5738179.1"
                     /translation="MASTEASKNKLASLTDCMTRVAEQRDKSAFAELFDHYAPQLRAY
                     SLAREPGADLVADELVQEVMTRVWLKADKFDSRLANLNTWIFTLARNCRVDYLRRNSR
                     YVTEIDPTDIFDNIEYEGPSPFQMAHQSRAEERIRAGLKALPREQSEILTTVYLEGKS
                     HQQASEELQLPLGTVKSRVRLALKKLEVLVER"
     gene            4907..5572
                     /locus_tag="VIW27_00570"
     CDS             4907..5572
                     /locus_tag="VIW27_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HEY5738180.1"
                     /translation="MEKVSNHPADELLAAYSAGSLPLSQALCIAAHLEHCHSCMHELQ
                     QLNRVGTELMQQLEPAPTAEHLKQDLLARLDSLEDDIPDTTTTVAADSSIPRCLRQFV
                     ASDYQSLRWKRVSPGIQTVELCRDSNGARVELLKIKPGSSAVTHSHSGDEYTVILEGS
                     FSDEHGLYRQGDFVVKHVNDRHTPVATLDRECICLAVTEGPIQLTGFLGRLLNPFLRR
                     SHA"
     gene            5592..6545
                     /locus_tag="VIW27_00575"
     CDS             5592..6545
                     /locus_tag="VIW27_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEY5738181.1"
                     /translation="MSAQQDKILIGVSSCLIGEKVRFDGGHKQSRYLLDTLGQYFAFR
                     PFCPEMAIGLGVPRETIRLADIDGRVRAIGNKSPDLDVTDALLDCAQQQAGWHRDLFG
                     YVVKKDSPTCGMERVRVYHGEHPQRNGTGLYTQVMMRNFPALPVEEEGRLGDPVLRES
                     FVKRVFIYKRWHDLLDAGLDWAALTDFHARHKLILYSHHQERGRQLGRELAQAHSQPV
                     EDYAPRYLDELMSVLRIYARRSNHVNVLEHIRGYLKQDLDKDDKQELTESIENYRLGR
                     LPLIVPLTLLRHHFRKHPDPYIERSYYMQPHPDELMLLNSL"
     gene            6566..7996
                     /locus_tag="VIW27_00580"
     CDS             6566..7996
                     /locus_tag="VIW27_00580"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY5738182.1"
                     /translation="MSETPLALMWFRRDLRIGDNPALRAAAASGRVVPIYILDDVNAG
                     DWAMGAASRAWLHHSLQSLDRALGGGLRCFAGDAHEIIAALVTQLPVGGVYWNRCYEP
                     WRRERDTRIKAELGERGIDVESCNASLLWEPWQVCKPDGDPYRVFTPFYRKGCLSREA
                     PREPLPPPGDLPLYDGSVKGAVELAALDLLPREDWHRRMLEHWQIGEDAACERLETFC
                     DSALTDYRDGRDFPAREATSRLSPHLHFGELSPQQAWHRAEQASLHLDSDGPAHFKRE
                     LAWREFSYHLLYHFDDLPRSNFNSRFDNFEWLQNAAGLAAWQRGQTGFPIVDAGMREL
                     WQTGYMHNRVRMIVASFLVKNLGLHWHHGEDWFWDCLVDADLANNSASWQWVAGSGAD
                     AAPYFRIFNPLLQSAKFDPDGDYLARYCPELAGLPARFRHCPWEAPERTLREAGVELG
                     VDYPRPIVDLKVTRERALARFKALPA"
     gene            complement(8003..>9268)
                     /locus_tag="VIW27_00585"
     CDS             complement(8003..>9268)
                     /locus_tag="VIW27_00585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5738183.1"
                     /translation="WVPMVTAVVMLIVGHLILGMRRLVGSRLDRLQSDLSAANRQLGL
                     SLHAQGSLDLAFEKFRACLVEDSLLGQVYNLGLDYERKRQFNKAASVFRFIHKQDAKF
                     NDVERRMEQNEQAAQSLVLGGRDRGGPGPNLISSKSGIEKPMLGRYQIDSEIGRGAMG
                     MVYLGHDDRIGRTVAIKTVGLSEDIEPAVREQVRNRFFREAEAAGRLNHPNIVTVYDI
                     GEEQDLAYIAMDYLKGKDLTAYCSPDTLLPASQVFEIIIGIARALDYAHQQHVVHRDV
                     KPANIIYDDEKRLAKITDFGVACLTDASKTKTGTVIGSPYYMAPEQLEGKKVDGHADL
                     FSLGVTLYQLLTGELPFKGDSIANLMYNIANEKHADVRKFRTDLPRCASTIVDKALQK
                     ESRARYKSGKTMAEAMIACQQQIREVDAA"
BASE COUNT         1809 a         3010 c         2782 g         1667 t
ORIGIN      
        1 gatcttcatg gtaatgaatg cgctcgcccc acttcaggtg gctcaccccg cgcaggccct
       61 ggcgaggatc gctcgagcgc gaatgaccgg catgcaggat ccatttcagg taggcggaat
      121 tttcaccgac gatctcgtcg agaaactcga atcggcaaac cccggcgtcg tcgatcagcg
      181 cggcgtggta atcctccagc tggaccagcc cccggagctt gcgcgacggt gcctggtaga
      241 ctacgtcatc ggcatacagc gcgtgcagtc ggctggggtc ggaacggtgt aattcccgat
      301 aataggcctt gaaacggtcg atgaacgctc ggttcgaatt cataacagga aatgtctctc
      361 actgccggat ttattgaagt ttgtatacgc tccggcaccc ccaacggatc atcgacagcc
      421 cgcgccttac cagcgctgcg gtaccctctc gacgagcgag atatcatagc cttgcgcgga
      481 gagaaaggcg agcatatcgg ccagttcgcg ctcgtcgata gccggttcgc gcgccattat
      541 ccaggcgtaa tctcgctggc tgcggccgat aatcgcgcgg gtgtagtcat cggtcaggta
      601 aacgatgcgg tactcggcct cgaacggcca gaaaaaccga atctgccaga ccgcattggt
      661 ttcccggtcc tccacgaagc cgcgcgcatc gtaaacctcg cgcggcccgt cgaagccgcc
      721 gtcgcgaaaa ctgaaacgcg ttgcgacact gccatcatcg tcgagccggt aggtttcgat
      781 cgcgttgtgg gcgccccgct cgaacacggt cggtatgctg gcaatcacgt accagtcccc
      841 catgaaacgc tgcagatcga cctgctgcac cgtggcgatc ggttgcggtg agccgcaggc
      901 agcgagccac gcgaccggca gagcggccgc gacgatacgc aacgcgcgcc gcaatttacc
      961 gccccgattc ccgcttcgcc ggcgcgccgt cgtcaccggc ggcgacttgc attgtcgagc
     1021 gcagggcttc gacctgctca cggttggcga gccggtagct gatcacccgg ccgtcgaccc
     1081 gcgattcgag cgacaaccag cggccatcct cgagcgcgtg ccagacggtg atatcgatct
     1141 cctgctcgct attgaccagc cgatggcggc tggcgctcac cgtgcggtcg ttgacctgca
     1201 attcctcgac cccttcagcg gtaatctcga cggccaggta ttcgccgttc tgcgcgttca
     1261 gcagtctgtt ctggcgcaga aagtcccggt cccagtaggc aaaagacatc acgcaaccgg
     1321 catcgagctc ggccgcgccg tcgagcgtcg atacttcgaa ctgcgcgccg gtatcacggc
     1381 cgtcgacgcg aaaatcgtcg ccgccgtcct cggtcgtcga aacgatcgat tcgagacagc
     1441 cctgccgcca ggtctcgcgg ttgcgatggt cgtagcggta cacgggtatg cccagaaagg
     1501 tgaccttgaa tctggcgtcg atatcgatga cttgctgctg ttcccgctgc atcacgtgga
     1561 agcggtgata gccgatctcg ctatcatcga gcagcactcg aaataccagc tcctggtccg
     1621 ccctcggtgc cgcctgcagc cagccgctgg cggccgccag cgacagcccg gccacgacgc
     1681 tcgcgattcg ccgcttcatg accggctcac cgattggtcg ccgacaccgc tgcgcttcgg
     1741 cggcagcggc acgaaggcgc tggtacgcac gatatattcg gcgtaggccg ggcgccgttc
     1801 gccgatgtcc tgttccagca gcgacacgcc ggacaccttg agcagcagga aggtcatcag
     1861 caccggcgac agcaacgccc accaggcacc ggcggcaccc gccatcaggc cgaagcccca
     1921 ccagatacag gcgttgccga aataattggg gtggcgcgta tagcgccaca ggccgcggtc
     1981 catgaccttg ccgcgattgg ccggatcggc cttgaaccgc gccagctgga aatcgccacc
     2041 ggcctcgaac agcatgccga taccccacag ggccaggccc aggtaatcga gggcgccgag
     2101 cggcgccgcg ccgccgattg cgatcaacag cggcaacgcc acgatccacg cgatcacgcc
     2161 ctgcaaaacg aacacgatta gcaggctctt gagttcgaag ttgggctggt tattggcgcg
     2221 aatctgctgg tagcggcggt cttcttcctc accccagttg cgccaggtaa tataggcaaa
     2281 cagtcgcagc gcccagatcg cgaccagcac cagcagcagc accgcgcgcg gcccggtatc
     2341 ggtcagcgtc aggtaaaagg tgagcgcggc aaacaggaac atcagcggcc aaaggctgtc
     2401 gacgatgctg acgtcacgct tcagcaggct gaccagccag acgccgacgc cggcggtcag
     2461 gatgacgccc agcgcgatca ggtaagcggt tagatcgaac atttcagccc tccccggcgg
     2521 ccagcgcggc gcgcgccgga atggtttcag ggtaaccgtt gaagcgcttc gcgagcagca
     2581 tcatcgacgg catcaacacc atccagccga gccccagcgc ggtcagcgcg atcgtcgtat
     2641 cggaaaactc gacaccaccg agcctcgagc cggcgtaaaa ggccagcggg ccgccggcgg
     2701 cgccgaacag cgccgccagc aacagccgct gcttgaacca gcgcatgctg agattgaacg
     2761 tggtcgcgaa taccacccac agagccacga tccagtaggg cgcggtaccg gcgaccaacg
     2821 tgcctgacgg ataaaccagc caacccgcgg ccaccagcag gctctcccag accacaccga
     2881 tcagcgccgc tgccaggatc agggtcccct cagcgcgggc atcttcggcg cgcagcaggt
     2941 ggtaggcgac ggcggcggcg acgaagccga cgccgagcag cggcatgccg ttggcaccgc
     3001 caaggataca aactatccag caaatctgga aaatgacgaa gttgataaac agtttcacgg
     3061 tattcctcaa tcgttcccgg ggcgctcgaa aaggtagtgg ctgacccacc attcacgacc
     3121 ttcgtggtag ccaaaaagct cggcgcaagc catgaaaaac actcgccagc gcatccacca
     3181 gaccgctgcc tggtcgccgt agacgctctc gagtatcggc atcagctgcg gcttgcgcgc
     3241 gtccatcttc gccaaccacg cattcagggt cttttcgtaa tggcggccgt tccagcgcca
     3301 ttgctgccgc agcctgagat catcctggaa tttcagtggc aacgcgtcgc tgggcatgat
     3361 gccgccgctg aagaagtgac ggctcatcca gtcgctggca tcgcgttcga cgaactcgta
     3421 cggaatcagc cggtgacaga atatgtgcat gaaaaaacgc ccgcccggtc gaagccagtc
     3481 atgcacgcgg gcgaacaacc gccggtaatt gcgcatgtgc tcgaacattt ccagcgaaac
     3541 gacgcgatcg aactgttggt cgatatcaaa atcgttcatg tcagcggtaa tgacccgcag
     3601 gttgtcgagt ccttcgcgag tggcgacctc ctcgatgtag gcgcgctgcc ccgaagaatt
     3661 ggataccgcg gtgaagcgcg cggccgggta cttgcgagcc atccacaggg ttagagaccc
     3721 ccagccgcag ccgagttcga gaatttgcat accgtcggcg atacccgcgc gctcgcaggt
     3781 aatacgcagc gattcgctct cggcgcgctc cagggtctgc tcgccgggtc cccaatagca
     3841 actgctgtac ttgcgctgcg ggccaagcac ctcgacgaaa aattcagcgg gcagctcgta
     3901 gtgctgttcg ttggcctttt ccggcagtgg cgcgatcggc gaagcgtcca tcattcgcac
     3961 gaactcttcg gcgcgcacgg cggccgactc gatatcttca gcgtcgattt cctgtaaacg
     4021 ctgcgccagc aaacgccgga ttccggcacg taccacgcgg tcgggaacca ggccgagttc
     4081 gacccagccg agcgcgcggt cagcggcggc gctcacgatt ccgctccagc caaccggaaa
     4141 acgcatttcc gggacgctcc cttaacggtt atcagaagca acataactgg cattatcctc
     4201 gaaaaaatca gcaagcgaac cctgtgtacg ggttttcgga ggttctggat cattcgagtg
     4261 atccgcgtca accgcattgc cgtataatag ccacttaaca ttactcaccg gcacctatgc
     4321 gaaccatggc aagcaccgag gcgtcaaaaa acaagctggc ctcgctcacc gactgcatga
     4381 caagagtcgc cgagcaacgt gacaagtcag cattcgcaga attgttcgac cactacgcgc
     4441 cgcaactccg cgcctacagc ctggcgcgcg agccgggcgc cgacctggtc gccgacgagc
     4501 tggtccagga ggtcatgacc cgcgtttggc tgaaagccga caagttcgat tcccggctag
     4561 ccaacttgaa tacctggatt ttcaccctgg cgcggaactg ccgcgtcgat tacctgcgac
     4621 gcaacagccg ttacgtcacc gaaatcgatc cgaccgacat cttcgacaac atcgaatacg
     4681 aagggccgag ccccttccag atggcgcatc agtcccgggc ggaagagaga ataagagcag
     4741 gcctcaaagc cttgccgcgc gaacagtcgg aaattctgac tactgtctac ctggagggga
     4801 agagtcacca acaggccagc gaagagcttc agctgccact tgggaccgtc aagtcccgcg
     4861 tacggctggc gctgaagaaa ctcgaagtac tggtagagag ataaccatgg aaaaagtatc
     4921 aaaccacccc gccgatgaat tgctggccgc ctattccgcc ggcagtttgc cactgagcca
     4981 ggcattgtgc atcgccgctc atctcgaaca ctgccatagt tgcatgcatg agttgcagca
     5041 gttgaaccgt gtcggtaccg aattgatgca acagctcgaa ccggcgccga ccgcggaaca
     5101 cctcaagcag gacttgctcg cgcggctcga ctcgctcgag gacgatatcc ccgacaccac
     5161 gaccacggtc gccgctgact cgtcgattcc gcgctgcctg cgccaattcg tcgccagcga
     5221 ctaccagagc ctgcgctgga agcgcgtttc ccccggtatc cagaccgtcg aactgtgtcg
     5281 tgatagcaat ggagcccgtg tcgagctgct gaagatcaag ccgggcagtt ctgccgtcac
     5341 tcactcgcat tccggcgatg aatacacggt catcctcgag ggcagctttt ccgacgagca
     5401 cggcctctac cgccagggcg atttcgtggt caagcacgtc aacgatcgcc ataccccggt
     5461 agccacgctg gatcgcgaat gcatttgcct cgcggtaacc gagggcccga ttcagttgac
     5521 cggctttttg ggccgcctgc tgaacccgtt tctgcggcgt agccacgcct gagcctggcg
     5581 gcgcacgatt ggtgagcgcg caacaagaca aaatcctgat cggcgtcagc agttgcctga
     5641 tcggggagaa ggtccgcttc gacggcggcc acaagcaaag tcgctacctg ctggacacgc
     5701 tcggacaata cttcgccttc cgccctttct gtccggagat ggcgatcggt ctcggcgtgc
     5761 cgcgcgagac catacgcctc gccgacatcg acggccgcgt cagggcgatc ggcaacaaga
     5821 gccccgatct cgatgtcacc gacgcgctgc tcgattgcgc ccagcagcag gctggctggc
     5881 accgcgacct gttcggctat gtcgtcaaga aggattcccc gacctgcggc atggagcggg
     5941 tgcgtgtcta ccacggcgag cacccgcagc gcaacggcac cgggctctac acgcaggtca
     6001 tgatgcgcaa ctttccggcg ctaccggtcg aggaagaggg gcggctcggc gacccggtgc
     6061 tgcgcgaaag tttcgtcaag cgggtcttta tttacaagcg ctggcacgac ctgctcgatg
     6121 cgggactcga ctgggccgcg ctgaccgatt ttcacgcgcg tcacaagctg atcctgtaca
     6181 gccatcacca ggaacgcggc cgccagctgg gccgcgaact ggcgcaggca cacagccaac
     6241 cggtcgagga ttacgcgcca cgttacctcg atgagttgat gagcgttctt cggatctacg
     6301 cgcggcgctc caaccacgtc aacgtgctcg agcacattcg cggctacctg aagcaggacc
     6361 tcgacaagga tgacaagcag gagctgaccg agagcatcga gaactaccgc ctcgggcgcc
     6421 tgccgctgat tgtgccgcta accctgctgc ggcatcactt ccgcaagcat cccgatccct
     6481 acatagaacg ctcttactac atgcagccgc atcccgacga gctgatgctg ctgaactcgt
     6541 tgtagcccgg cgctagcaac cgaagatgag tgagactccg ctagccctga tgtggtttcg
     6601 ccgcgatctg cgtatcggcg acaacccggc gctgcgcgcg gccgccgcgt ccggacgcgt
     6661 ggtgccgatc tacattctcg acgacgtaaa cgccggcgac tgggcgatgg gcgccgcgag
     6721 ccgtgcctgg ctgcatcatt cgctgcaatc gctcgaccgc gcgctgggcg gtggcctgcg
     6781 ctgcttcgcc ggcgatgccc acgaaatcat tgccgcgctg gttacgcagc tgccggtcgg
     6841 tggggtttac tggaatcgct gctacgaacc ctggcgccgc gaacgcgaca ctcgaatcaa
     6901 ggcggagctc ggcgagcgcg gcatcgacgt agagagttgt aacgcgtctc tgttgtggga
     6961 accctggcag gtatgcaagc cggacggcga cccctaccgc gtgttcacgc cgttctaccg
     7021 caagggctgc ctgtcccgcg aagcaccccg cgaacccctg ccgccgcccg gcgatctgcc
     7081 gctgtacgat ggatcggtca agggcgccgt cgagttggcg gcgctcgacc tgctgccgcg
     7141 cgaggactgg caccgccgca tgctcgagca ctggcagatc ggcgaggatg cggcgtgcga
     7201 aaggctcgag acgttttgcg attcggcgct caccgattat cgcgacggcc gcgattttcc
     7261 ggcgcgcgaa gcgacttcga gactctcgcc gcatctgcat ttcggcgagc tgtcaccaca
     7321 gcaggcctgg caccgggccg agcaggccag cctgcatctg gatagcgacg gacccgccca
     7381 tttcaagcga gaactggcat ggcgggagtt ttcctatcac ctgctgtacc atttcgatga
     7441 cttgccgcga tcgaacttca attcgcgctt cgacaacttc gagtggctac aaaatgccgc
     7501 ggggctcgcc gcctggcagc gcgggcagac cgggtttccg atcgtcgacg ccggcatgcg
     7561 cgagctgtgg cagaccggct acatgcacaa tcgcgtgcgc atgatcgtcg cttccttcct
     7621 ggtcaagaac ctcgggctgc actggcatca cggtgaagac tggttctggg actgcctggt
     7681 cgacgccgat ctcgccaata acagcgcgag ctggcaatgg gtggccggtt cgggcgccga
     7741 tgccgcgcct tactttcgca tcttcaatcc gctgctgcag agcgccaaat tcgatcccga
     7801 cggcgactat ttagcgcgtt actgcccgga gctggctggc ttgccggcca ggttccgcca
     7861 ctgcccctgg gaggcaccgg agagaaccct gcgcgaggcc ggggtcgagc tcggcgtcga
     7921 ttacccgcgg ccgattgtcg atttgaaggt tacccgcgag cgtgcgctgg cgcgcttcaa
     7981 ggcactgccc gcttagccgc gctcaggcgg cgtccacttc ccgaatctgt tgctggcagg
     8041 cgatcatcgc ctcggccatc gtcttgccgc tcttgtacct ggctcgcgat tccttctgca
     8101 gcgccttgtc aacaatagtg ctcgcgcatc gcggcaggtc ggtgcggaac ttgcgcacgt
     8161 cggcgtgttt ctcgttggcg atgttgtaca tcaggttagc gatggaatcg cccttgaaag
     8221 gcaattcgcc ggtcaacagc tggtacagcg tcacaccgag cgagaacagg tcggcatggc
     8281 cgtcgacctt tttgccctcc agctgctcgg gtgccatgta atacggactg ccgatcaccg
     8341 tgccggtctt ggtcttgctg gcatcggtga ggcaggcgac gccgaagtcg gtaatcttgg
     8401 cgagccgttt ttcgtcgtcg tagatgatat tcgccggctt gacgtcgcgg tgcacgacat
     8461 gctgctggtg ggcgtaatcc agcgccctgg caatgccaat gatgatttcg aatacctggc
     8521 tggctggcaa cagcgtatcc ggcgagcaat aagcggtcag gtccttgccc ttcaggtaat
     8581 ccatcgctat ataggccagg tcctgctctt cgccgatgtc atagacggtg acgatattcg
     8641 ggtggttgag gcggccggct gcctccgcct cgcgaaagaa gcgattacgc acctgctcgc
     8701 gcacggcggg ctcgatatcc tccgacagcc ccacggtctt gatcgccacg gtacgtccga
     8761 tcctgtcgtc gtggccgagg tataccatgc ccatcgcgcc gcggccgatt tcactgtcga
     8821 tctggtagcg gccgagcatg ggtttctcga taccggactt gctcgagatc aggttcggcc
     8881 ccggcccccc gcggtcgcga ccgccgagca ccagcgactg ggcggcttgc tcgttttgct
     8941 ccatgcggcg ttcgacgtcg ttgaacttcg catcctgctt gtggataaaa cggaacaccg
     9001 atgccgcctt gttgaactgg cgcttgcgct cgtagtcgag gccgaggttg tagacctggc
     9061 cgagcagcga gtcttcgacc aggcaggcgc gaaacttttc gaaggccagg tccaggctgc
     9121 cctgggcatg cagcgacagg ccgagctggc ggttggccgc cgacaggtcg ctctgcagcc
     9181 ggtcgaggcg cgatccgacc agccggcgca tgcccagtat caggtggccg acgatgagca
     9241 tcaccaccgc ggtcaccatc ggcaccca
//
LOCUS       DATCZV010000026         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_531275, whole genome shotgun sequence.
ACCESSION   DATCZV010000026 DATCZV010000000
VERSION     DATCZV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531275"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="nuoF"
                     /locus_tag="VIW27_00590"
     CDS             <1..991
                     /gene="nuoF"
                     /locus_tag="VIW27_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5738184.1"
                     /translation="RYNPHCLVEGMAIAAYAMGATVAYNYMRGEFMDEPAQRFETAVR
                     EAYAAGYLGKNILSSGVDFDLYPALGAGAYICGEETALLESLEGKKGQPRYKPPFPAI
                     FGLYGKPTTINNTESLASTPSIIRNGALWFAELGIPNNGGVKCFSVSGHLNKPGNFEI
                     SMGTPFRDLLEMAGGVRDGHRLKAVIPGGSSVPVLPGEVMMECTMDYDSISKAGSMLG
                     SGAVIVMDETTDMVLALQRISRFYYSESCGQCTPCREGTGWLYRMLTRIVEGKGRPED
                     IQLLEEVSHKIEGRTICALGDAAAMPVWSFIKHFRHEFEYYVEHKRSIVDNAA"
     gene            1010..>2053
                     /gene="nuoG"
                     /locus_tag="VIW27_00595"
     CDS             1010..>2053
                     /gene="nuoG"
                     /locus_tag="VIW27_00595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY5738185.1"
                     /translation="MSDNITITINGQTIETQPGRLLIDVADENNIVIPRFCYHKRLSV
                     AANCRMCLVEIERAPKPMPACATQCMDGMVVQTRSPGALAAQKSTMEFLLINHPLDCP
                     ICDQGGECELQDVAMGFGEGISKYTESKRVVMDKNIGPLIATDFTRCIHCTRCVRFGD
                     EIAGMPELGATGRGEHMEIGTYIEKSIVSELSGNVIDVCPVGALTAKPSRYTARPWEL
                     TQHASVSAHDCVGSNTFVHTRGNEVIRVVPRENESINESWISDRDRFSYTAVKAEQRI
                     TAPMLRDNGELRAVDWQTALDTAANRLSDAGGSIAALVSPQATLEEHYLAQKLLRGLG
                     SNSIDHRLTQVDFS"
BASE COUNT          434 a          608 c          623 g          388 t
ORIGIN      
        1 gcggtataac ccgcattgcc tggttgaagg catggccata gcggcctacg cgatgggggc
       61 tacagttgcg tacaactaca tgcgtggcga attcatggac gagccggcgc agcgcttcga
      121 aaccgcggtc agggaagcct acgcggcggg ctacctcggc aagaatatcc ttagcagcgg
      181 tgtcgatttc gatttatacc cggcactggg cgccggcgcc tatatttgcg gcgaggaaac
      241 cgcgctgctc gaatcgctcg aaggcaagaa gggccagccc cgctacaagc caccgttccc
      301 ggcaatcttc gggctgtatg gcaagccaac cacgatcaac aacaccgaat ccctggcctc
      361 gacgccgtcg atcatccgca acggcgcgct atggttcgcc gaactcggca ttcccaacaa
      421 tggtggggtt aagtgctttt cggtgtctgg acatttaaac aagccgggca atttcgaaat
      481 ttcgatgggg acacccttca gggatctgtt ggaaatggcc ggcggagtgc gtgacgggca
      541 ccgcctcaaa gcggtgattc ccggcggatc ctcggtgccg gtactgccgg gcgaggtcat
      601 gatggaatgc accatggatt acgattcgat ctcgaaggcg ggatcgatgc tgggctcggg
      661 cgcggtcatc gttatggatg aaaccaccga catggtgctc gcgctgcaac gtatctcgcg
      721 tttttactac tccgaatcct gtggccagtg cacgccctgt cgcgagggca ccggctggct
      781 ttaccgcatg ctgacgcgta tcgtcgaagg caagggccgg cccgaggaca tacagctgct
      841 ggaagaggtg tcgcacaaga tcgagggcag gactatttgc gccctcggcg atgccgcggc
      901 gatgccggtg tggagtttca tcaagcactt ccggcatgaa ttcgaatact acgtcgagca
      961 caagcgctcg attgtcgata acgcggcctg atcggatcga ccaactgcaa tgagcgataa
     1021 cataacaata accataaacg gccagacgat cgaaacccag cccggacgtc tgctgatcga
     1081 tgtggccgat gaaaataaca tcgtgattcc acggttttgt taccacaagc gcctgtcggt
     1141 cgcggccaac tgccgcatgt gcctggtcga aatcgagcgt gcgcccaagc cgatgccggc
     1201 ctgcgctacc cagtgcatgg acggtatggt ggtgcagacc cgctcgccgg gcgcgctggc
     1261 cgcccagaaa tcgaccatgg agttcctgtt gatcaaccac ccgctcgatt gcccgatctg
     1321 cgaccagggt ggtgagtgcg aactgcagga cgtggcgatg ggtttcggcg agggcatctc
     1381 caagtacacc gaatcgaaac gcgtggtcat ggacaagaat atcgggccgc tgatcgcgac
     1441 cgatttcacg cgctgtattc actgcactcg ctgcgtgcgc ttcggcgatg aaatcgccgg
     1501 catgccggaa ctcggcgcta ccgggcgcgg cgaacacatg gaaatcggca cctatatcga
     1561 gaagtcgatc gtctccgagc tgtccggcaa cgtcatcgac gtctgcccgg tgggcgcgct
     1621 gaccgccaag ccgtctcgtt ataccgcgcg accctgggaa ttgacccagc atgcgtcggt
     1681 atcggcgcac gactgtgtcg gctccaacac cttcgtgcac acccgcggca acgaagttat
     1741 tcgagtggtg ccgcgggaaa acgaatcgat caatgaatcc tggatctcgg accgcgatcg
     1801 cttcagctat accgcggtca aggccgagca gcgtattacc gcaccgatgc tgcgcgacaa
     1861 tggagaactg agggcggtcg actggcaaac ggcgctcgac accgcggcca accgtctatc
     1921 cgatgcaggc ggcagcatag cggcgctagt cagcccgcag gcgacgctgg aagagcatta
     1981 cctggcgcag aagctgctac gtggcctcgg ttccaacagt atcgatcatc gactcaccca
     2041 ggtggacttt tct
//
LOCUS       DATCZV010000027         9181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_584428, whole genome shotgun sequence.
ACCESSION   DATCZV010000027 DATCZV010000000
VERSION     DATCZV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584428"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..864)
                     /locus_tag="VIW27_00600"
     CDS             complement(127..864)
                     /locus_tag="VIW27_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY5738186.1"
                     /translation="MGSRPLAMVLACAMFACMPRFATCADLQAAIRNGDSAALERALM
                     STPREEAGLFLHLAIIMEDLQAIRLLHEHGADLEASNRDSGAHPLHVAADIGMPPVVN
                     LLVDLGAELEARDSLGRTPLIQAAIAGNSAVVSVLLAAGANADAAESFHGTTALHLAA
                     FRDRREIIDALLAYGAAVDVADKQGETPLFWAVGRGHAQAITLLVNAGADVNHRNHAG
                     ISPLGAAAFAHDREATARLLKILGAEE"
     gene            complement(1278..1670)
                     /locus_tag="VIW27_00605"
     CDS             complement(1278..1670)
                     /locus_tag="VIW27_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738187.1"
                     /translation="MGGTGANPGDVDPLALLRSSGIDTDLIPAPLLPTIASSLAMNTD
                     ETNDQAKEQAKIKVDCGSCNREVAYGKGKCMYCGKPLELPFSATGPDVPLDSATVDND
                     GADGDSRDTGAGKSTTSTGIDFDLGGSL"
     gene            complement(2036..2278)
                     /locus_tag="VIW27_00610"
     CDS             complement(2036..2278)
                     /locus_tag="VIW27_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738188.1"
                     /translation="MLVIDREAQLLLSDRSAGSLAAVRKEMLSQLTAGDRVQFGYRTP
                     DDGPAAVIEFEITHHASKTAATEVAEDIVLAFDRRA"
     gene            complement(2316..2663)
                     /locus_tag="VIW27_00615"
     CDS             complement(2316..2663)
                     /locus_tag="VIW27_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738189.1"
                     /translation="MPVRKSRPLLAAAVLLVAGGCAGTDLGRIDETRDERDDMAGPGV
                     FADQSGETPLKWTVGDTRPSATEPTLELALDEQVEFELFKEWRRLRAEGSDSPEYREF
                     LQWLEFREFKDAQ"
     gene            complement(2663..3703)
                     /locus_tag="VIW27_00620"
     CDS             complement(2663..3703)
                     /locus_tag="VIW27_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY5738190.1"
                     /translation="MQSKALTRYLPVTLCSAAALALSTPVAAVDFTISGQLNRLIMSV
                     DNGVEDGIVHADNSASGTRLRFEGMGETDGGTEVGLYYETQLQSNPSDKVSVDSLDGD
                     GSGGNVGEGDYFSTRFATVWFKGDFGKLTIGQGSGATDGVAEVDKSGTTVIQYVGASN
                     DLLGSMEYGASGVTVGQARGHFDGLGRNDNLRYDASTGEISFAASIGNGDKTELSAGY
                     AVDNLEIRVGIWDSADSGNDVAGNAISASWIGAGGFNLTASYGSDDRAGDPTNIYFKV
                     GYRAGAAAYAIDWGETSDLTDGDASAMSVAYVRDLMQGVQVYASYRTDSLDGVAGEDD
                     ITALVGGARVKF"
     gene            3886..5472
                     /locus_tag="VIW27_00625"
     CDS             3886..5472
                     /locus_tag="VIW27_00625"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY5738191.1"
                     /translation="MACRRHILLPLLALTLLSPPAAAETLLAEGMREIEEFRMRQLQA
                     VQADAEVAPFTTDGCSGNQSKSWEIMARVIPAFEAEFGERPPWEACCVAHDELYWRGL
                     VDDGFNRRIAADRELQACVAATGVRLSPQLSLRYDVSAERVRDAFATISEWMYRAVRI
                     GGQPCSLLPWRWGYGWPNCAFAMAANAPPGVSDIKADERVVFFNTAAWLDEARMQWNI
                     PLHAWVYEAESSTLRKGVFATALEIEYGLVATPDSQALFDRRTNLLIADNERGKTLLV
                     RIAGQDIKLPESAANGQSSSVLQFPADLIDAIADRGRIRFFAVTAAQETRRFEGEVRL
                     VSPRGVSVISDIDDTVKVSHVTDQRLLLASTFFNPFEAVPGMPELYRQLAAGGASLHF
                     VSSSPWQLYEPLREFMDAAGFPWATLSLKAVRFRDATLLNLFKKGTETKPEQIVPLLE
                     RYPGRRFILIGDSGEQDPEVYGEIARRYPQRIQRILIRSLDDATRDATRYLTAFDQLP
                     PGKWQLFSDPGELSADDLLR"
     gene            complement(5619..6206)
                     /locus_tag="VIW27_00630"
     CDS             complement(5619..6206)
                     /locus_tag="VIW27_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEY5738192.1"
                     /translation="MRYLWLLVFFGVLAWSGIAPKDRFTWLLEVSPALIGLALLAFTW
                     HSFRLTPLLYLLILLHSIVLMVGGHYTYAEVPLFDNLFGFERNNYDKVGHFFQGFVPA
                     LLAREILLRLQVVDGDAWRNLFIVSICLAFSAFYELIEWWVALASGEGAEAFLGTQGY
                     VWDTQSDMALALLGAICSLALLSKLHDTQLASLRT"
     gene            complement(6262..6657)
                     /locus_tag="VIW27_00635"
     CDS             complement(6262..6657)
                     /locus_tag="VIW27_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEY5738193.1"
                     /translation="MTDQRHQITVYRSATCGCCKGWIAHLREHNFDVKDVTVSDVEPH
                     KRELGVPPQAASCHTAVVEGVTIEGHVPAQDIKRLLSGDHNQRLLTVPAMPSGSPGMD
                     TPDAPKHAFKVFAISNDDQVSVFNEYTNY"
     gene            complement(6837..7775)
                     /locus_tag="VIW27_00640"
     CDS             complement(6837..7775)
                     /locus_tag="VIW27_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY5738194.1"
                     /translation="MYPGKPPSAPDTRVLRGALLVLLALWYATPVAAVSYADIAPILA
                     ERCTLCHAGDAAPLGLRLDSLDDLLRGSRNGPVVEPGDPGASELIRRLTGDSQPRMPM
                     TGPPFLSASQVAMFVDWIAGGLQPGDAAAAVAPPAAPSRPNPGEPVTWVHVAPIFATR
                     CAKCHAQQGLMGPAPEGYRLDSYDAVLAAFDRLRVVPGLPNASELMRRIRGQALPRMP
                     FDGPPYLSGDEIGLVEDWIRDGARDAEGRPAAYPTGAKLRLHGTLESRWRLEGLPLAV
                     GAGARIDKAPAPGDYVEVRGRLGAENVVVVERIRRR"
     gene            8005..8172
                     /locus_tag="VIW27_00645"
     CDS             8005..8172
                     /locus_tag="VIW27_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HEY5738195.1"
                     /translation="MTPKEPEQVSCEVCLKEIPVDEAKSAEATDYVAHFCGLECFEKW
                     KQKEQPQEKGN"
     gene            8494..8901
                     /locus_tag="VIW27_00650"
     CDS             8494..8901
                     /locus_tag="VIW27_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5738196.1"
                     /translation="MRILDNLTGGEQPAFASNCNGRIVDWNQGAERLLGYSAATMIGR
                     HCFEVLDGKDVFGNRFCHERCAIRCMIRRHEPVHHWQLNYRTASSARIDVSVGAVVVN
                     GDESADSLIVHLLQPVDSRGNEFGPAIPLKPSE"
     gene            8936..9127
                     /locus_tag="VIW27_00655"
     CDS             8936..9127
                     /locus_tag="VIW27_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5738197.1"
                     /translation="MRLTARKHEILRLLAAGSSTSEIVHQLSINADTVRTHIRNIFYK
                     PNAYTRLEAVGKALRRRLL"
BASE COUNT         1801 a         2875 c         2804 g         1701 t
ORIGIN      
        1 gatcgacgat tgcaagcagc agggcggtct tgaccagatc ctgcccgacg acagcggtga
       61 acggaaaact ggccatcgac tccgcgttgt attgaaccgg agcgacatta tacccgttta
      121 cggaccctac tcctcggcgc cgagaatttt caacaggcgc gccgtggcct cgcggtcgtg
      181 ggcaaaggcg gcagcgccga gcggcgatat gccggcgtgg ttgcggtgat tgacgtcggc
      241 gccggcattg accagcaggg tgatggcttg cgcatggccg cggccgaccg cccagaacag
      301 cggggtctcg ccctgcttgt cggcaacatc gacagcggcg ccataagcca gcagcgcatc
      361 gatgatttcg cgccgatctc tgaacgcagc caggtgtaac gcggtggtac catgaaagct
      421 ttcggccgca tccgcattcg cccccgcggc gagcaatacc gagactaccg cgctgttgcc
      481 ggcgatcgcg gcctggatca gcggcgtgcg gccgaggctg tcgcgggctt cgagttcggc
      541 gccgagatcg accagcaggt tgaccaccgg cggcatgccg atgtcggcgg cgacatgcag
      601 cgggtgcgcg ccgctgtccc ggtttgacgc ctcgagatcg gcgccgtgtt catgcaacag
      661 acgtatcgcc tgcaagtctt ccatgattat cgccaggtgc aggaaaagtc cggcttcctc
      721 acggggcgtg gacatcagcg cacgctcgag cgcggcgctg tcgccgttgc ggattgccgc
      781 ctgcaaatcg gcgcaggtcg caaaccgcgg catgcaggca aacatcgcgc aggcaagcac
      841 catggcgagc ggccgactgc ccatactcat cccgttttta acatcgtcga taacacgtct
      901 cgccatcgag gctgattcac acaggtttcc gccacgccgc tgacgtctga aagtatagac
      961 gtttccggga attcgaatag acgcttcgcg gcattccatt cagggacagc gatgggaacg
     1021 cttcgatcat tgcccgttgc caggcgaaat gtggataccc tgccggtcgt ttttccgcgc
     1081 ggaggatagc aacagatgta tacaacctgc gtcggtcagg cacacgaata cgcgaagctc
     1141 cgagcgcggc gacaattatc cgcggccggt tctgaaacga agctataaat tctcgtggct
     1201 aaaaaatcgg cgtgatcggt aacgatccgc cgttgtccat cgattcgaaa accgaatcga
     1261 gggctgataa atccggccta aagtgagccg ccaaggtcga agtcgatgcc agtggaagtg
     1321 gtcgatttgc cggctcccgt atcgcggcta tcgccgtcgg cgccatcgtt gtcaaccgtg
     1381 gcgctatcca gcgggacatc cggaccggta gccgaaaacg gcagttccag cggcttgccg
     1441 caatacatgc attttccctt gccataggca acctcgcggt tacaggagcc gcaatccacc
     1501 ttgatcttgg cctgctcttt cgcctggtcg ttcgtttcgt cggtattcat cgccagtgaa
     1561 ctcgcaatcg tgggcagcag tggcgccgga atcaagtcgg tatcgatacc gctgcttctg
     1621 agcagcgcca gcggatcgac atcgccggga ttcgcgcctg tcccgcccac cgcgccactc
     1681 gcggctgccg ggataccgcc attgagaaac aggtctgtaa catcgggctt ccgtttcttc
     1741 ctgaaaaaca tggctcgatc gatgtcctcg cttgtcctga accgtcagcc tggcggccgc
     1801 gagcgcggat tccgtccaat tcgcgaactc cggccgcagt catgcccgcc accaggactt
     1861 tcaagctgtt attttagtat cagattcgcg gattttaccg acctttgaga aggtttttcg
     1921 caccggtgat cgggtggcca catggctact tgccgcgagt cactgaaaag tgacgatcag
     1981 atcttcgacc gtgatgctgc cgttggggcc gggacgatac tcgatgcgaa cccggttacg
     2041 cgcgccggtc gaaggcgagg acaatgtcct cggcgacctc cgtggcagcc gtcttcgagg
     2101 catggtgcgt gatttcgaac tcgattacag ccgccggccc gtcgtcgggt gtccggtagc
     2161 cgaactgtac tctatcgcct gcggtcagct ggcttaacat ctccttgcgg acagccgcca
     2221 gcgacccggc gctgcgatcg gacagcagca gttgcgcctc acgatcgatg accagcacct
     2281 tgccgacctc gatacccagc gattacgccg ccacactact gcgcgtcctt gaattcgcgg
     2341 aattccagcc actgcaggaa ttcccggtac tctggcgaat cgctaccctc cgcccgcagt
     2401 cgtcgccatt ccttgaacaa ctcgaactcg acctgctcgt ctagcgcgag ttcgagcgtc
     2461 ggctccgtgg cagacggccg cgtatcaccg accgtccact tcaacggtgt ctccccgctc
     2521 tggtccgcaa atacgccggg gccggccatg tcgtcgcgct cgtcgcgggt ctcgtcgatg
     2581 cgcccgagat cggtgccagc acagccgccg gcgaccagca acacggccgc ggccagcaac
     2641 ggccgggatt tacgcacagg catcagaact tgacccgggc gcctccgacc agcgcagtga
     2701 tgtcgtcctc gccggcgacg ccgtcaagac tgtcggtacg gtagctggcg tatacctgca
     2761 caccctgcat caggtcgcgc acataggcca cgctcattgc gctggcatcg ccgtcggtga
     2821 ggtcgctggt ctcgccccag tcgatcgcat aggccgcggc gccggcgcga taaccgacct
     2881 tgaaatagat gtttgtcggg tccccggcgc ggtcgtcgga tccgtaggag gcagtcaggt
     2941 tgaaaccgcc ggcgccgatc cagctcgccg agatcgcgtt gccagccacg tcgttgccgc
     3001 tatcggccga gtcccagatg ccgacgcgaa tctcgagatt gtcgaccgcg tagccggccg
     3061 acaattccgt cttgtcgccg ttaccgatgc tggcggcgaa agaaatctcg ccggtcgacg
     3121 catcgtaacg caggttatcg ttacgcccca ggccgtcgaa atgcccgcgg gcctggccga
     3181 ccgttactcc cgaggcaccg tactccatcg agccgagcaa gtcgttgctg gcaccgacgt
     3241 actggatgac cgtggtgccc gacttgtcga cctcggcgac accgtcggtg gcgccggatc
     3301 cctggccgat ggtcagcttg ccgaaatcgc ccttgaacca gacggtcgcg aaacgcgtcg
     3361 aaaaataatc tccctcaccg acgtttccgc cgctgccgtc gccgtcgaga ctgtctacac
     3421 tgaccttgtc cgacggattg ctctgcagct gcgtctcgta gtacaggccg acctcagtac
     3481 caccgtcggt ttcgcccata ccctcgaacc gcaggcgcgt gccgctggca gagttgtcgg
     3541 catgcacgat gccatcttcg acgccgttgt cgacgctcat gatcagccgg ttcagctggc
     3601 cggaaatcgt gaaatcgacc gcggcgacag gggtggaaag cgccagcgca gcggccgagc
     3661 acagggtgac aggcaaatac ctggttaacg cttttgattg catttatcgg gtcccatggt
     3721 tttttcgatt agacgtgtag tgctcaatga tataatcctg acgcggcgag aggaataaca
     3781 aatcgtcccc ggtgccgaat acgaggcttg ccgcttcggc accaggcctg ctgcacaggc
     3841 atcactttca ctcagcgaaa aattacaaat agatacactg cgtccatggc ctgccgtcga
     3901 catatcctgt taccgctgct agcgctgacc ttgctgtcgc cgccggccgc ggcagagacc
     3961 ctgctcgccg agggcatgcg cgagatcgag gaattccgca tgcgccaatt gcaggcagtg
     4021 caggccgacg ccgaggtcgc gccgttcacc accgacggct gcagcggcaa ccagtcgaag
     4081 agctgggaaa tcatggcccg ggtgataccc gccttcgagg ccgagttcgg cgagcggcca
     4141 ccgtgggaag cctgttgcgt cgcacacgac gaactctact ggcgaggact ggtcgacgac
     4201 ggcttcaatc gccgcatcgc cgccgaccgc gagctgcagg cctgcgtggc tgcgaccgga
     4261 gtgcgtctct cgccgcaact gagtcttcgc tatgatgtat ccgcggagcg ggttcgtgat
     4321 gccttcgcga cgatatccga gtggatgtac agagcggtac gcatcggcgg ccaaccgtgc
     4381 tcattgctgc cgtggcgctg gggttacggc tggccgaact gcgcctttgc catggcggcg
     4441 aatgccccgc ccggcgtttc cgatatcaag gccgacgagc gggtggtgtt tttcaacacc
     4501 gcggcatggc tcgacgaggc acgcatgcaa tggaatattc cgctgcacgc gtgggtctac
     4561 gaagcggaat ccagcacgct gcgcaagggg gtgttcgcca ccgcgctgga gatcgagtac
     4621 gggctggtgg ccactccgga ctcgcaggcg ttgttcgacc ggcggaccaa cctgctgatc
     4681 gccgacaacg agcgcggcaa gaccctcttg gtgcgcattg ccgggcagga catcaagctg
     4741 ccggagtcgg cggcaaacgg ccagtcgtcg agcgtgctgc aatttcccgc cgacttgatc
     4801 gacgcaatcg ccgatcgtgg ccgcatccgt tttttcgcgg tcaccgccgc gcaggaaacg
     4861 cggcgcttcg aaggtgaggt gcggctggta tcgccgcgcg gcgtcagcgt tatcagcgat
     4921 atcgacgaca cggttaaggt cagccacgtg accgatcagc ggctgttact cgcaagcact
     4981 tttttcaacc ccttcgaagc ggtgccggga atgccggagt tgtatcgtca gctggcggcc
     5041 ggcggcgcca gcctgcactt cgtctcctcg agcccgtggc aactttacga accactgcgc
     5101 gaattcatgg acgcggccgg attcccgtgg gcaaccctca gcctcaaagc ggtgcgtttt
     5161 cgcgacgcga ccttgctcaa cctgttcaag aaaggcaccg agaccaagcc ggagcaaatc
     5221 gttccgctgc tggaacgcta cccgggacgg cgattcattt tgatcggcga cagcggcgag
     5281 caggacccgg aagtctacgg tgaaatcgcg cgtcggtatc cgcagaggat tcagcgcatc
     5341 ctgattcgca gcctcgacga tgctacgcgt gacgccaccc gctacctgac cgccttcgac
     5401 cagctgccgc cgggcaagtg gcagctgttc tccgaccccg gcgagttgtc ggcggacgat
     5461 ctgctgcgtt gaaaaaagga cccggcgacg tgactcgatt ggctgtcatt gccgcggagg
     5521 cgggatgccg ccgggcggga acctcataac gcgccacaat tgcaggatgt ccgctcgctc
     5581 ggtcatgccg gccgaatgcg cggtcgcgga atcgccgctc aggttcgcag gctcgccagt
     5641 tgcgtgtcgt gcagcttgga cagcaatgca agcgagcaaa tcgcgcccag cagagccagc
     5701 gccatgtccg actgggtgtc ccagacgtag ccctgcgtgc cgagaaaggc ttccgcacct
     5761 tcgccactcg ccaacgcgac ccaccattcg atcagctcgt agaaggcgct gaacgccagg
     5821 cagatcgaaa cgatgaacag gttacgccac gcatctccgt cgaccacctg caggcgaagc
     5881 agaatttcgc gcgccagcag cgcgggcacg aagccctgga agaaatgacc gaccttgtcg
     5941 tagttgttgc gctcgaaacc gaacaggtta tcgaatagcg gaacttcggc gtaggtatag
     6001 tggccgccga ccatcaacac gatgctgtgc agcagaatca gcaggtacag cagcggcgtc
     6061 aacctgaacg aatgccaggt aaaggcgagc aaagccaggc cgatcaacgc cggcgacacc
     6121 tcgagcagcc aggtaaagcg atccttcggc gcgatgccag accaggccag tacgccgaaa
     6181 aataccagta gccagagata cctgatagca gcgctctcca gtcaatcggc gcggtcgaac
     6241 ctgccgacgc gccgagcacg atcagtaatt cgtatactcg ttgaaaacgc tgacctggtc
     6301 gtcattactg atcgcgaaga ccttgaaagc atgcttcggc gcatccggag tatccatgcc
     6361 gggcgagccc gacggcatcg ccggtacggt cagcaggcgc tggttgtggt cgcccgacag
     6421 cagccgcttg atatcctgcg cgggcacatg cccttcgatg gtcacgcctt cgacgaccgc
     6481 ggtgtggcac gacgccgcct gcggcggcac gccgagctcc cgtttgtgcg gctcgacgtc
     6541 gctcacggta acatccttga cgtcgaaatt gtgttccctg agatgcgcga tccagccctt
     6601 gcagcaaccg caggtcgcgc ttcggtatac ggttatctgg tggcgctgat cggtcattcc
     6661 ctgttgccag aattcgttcg tcgcaaacgc gggggacaga aaaaaaggca ggagcattgc
     6721 ggctgcatat ttcatcataa atacctcggt aaaaaatctc tgaacagtat actcgaatta
     6781 ccttggaggc cgccaaggcc gcaaggttcc gtggattttg cttcccggcg cttgcactag
     6841 cgacgcctga tgcgctcgac gacgaccacg ttttcggcgc cgaggcggcc acgaacctcg
     6901 acgtaatcgc ccggcgccgg cgccttgtcg atgcgcgcac ccgcgccgac tgcgagcggc
     6961 agtccttcga gccgccagcg cgactcgagc gtgccgtgca ggcgcagttt cgcgcccgtc
     7021 gggtaagccg ccggtctgcc ctcggcatcg cgcgcgccgt cgcgaatcca gtcctcgacc
     7081 agtccgatct cgtcgccgct caggtacggc gggccatcga acggcatgcg gggcagcgcc
     7141 tggccgcgaa tgcggcgcat aagctcactg gcgttcggca ggccgggcac cacgcgtagc
     7201 cgatcgaagg ccgccagcac agcgtcgtaa gagtccagcc ggtaaccttc cggggccggc
     7261 cccatcaagc cctgttgcgc atggcatttg gcgcagcgcg tggcgaagat cggcgccacg
     7321 tgaacccagg tgaccggctc gcccgggttc ggccgcgacg gcgcggccgg cggcgcgaca
     7381 gcagccgccg cgtcaccggg ctgcagaccg ccggcaatcc agtcgacgaa catcgccacc
     7441 tggcttgccg atagaaacgg cggcccggtc atcggcatgc gcggctggct gtcacccgtc
     7501 agccggcgaa tcagttcgct ggccccgggg tcacccggct cgacgaccgg accgttgcgg
     7561 ctgccgcgca gcaagtcgtc gaggctgtcg agcctcagac ccagcggcgc ggcatctccg
     7621 gcatggcaca aggtgcaacg ctcggcgagg atcggcgcga tgtccgcata actgacggcg
     7681 gcgaccggcg tcgcgtacca cagcgcaagc agcaccagca acgcgccccg aaggactcgt
     7741 gtatcgggtg ccgatggcgg tttgcctggg tacattgctg ttcgctccat cgatgaaaat
     7801 gaaccatatt atagcttttt cgaggctatc gaattactga tatcggcccg tcgggcgatt
     7861 ttcggcatgg cgtcttcggt attcgcggca aacggcgcgc aaaaattcgg cgtttgttgt
     7921 tagaagtcaa taagcgctgc ttcgaggggc aaataataat acgtgtcagg cgtgttggag
     7981 gggtgtgacc gtatgtccga aaagatgact cccaaagaac ccgaacaggt cagttgcgaa
     8041 gtttgcctga aggagatccc cgtggacgag gccaaaagcg cagaggcaac ggactatgtc
     8101 gctcatttct gcggcctcga gtgtttcgag aaatggaagc agaaggaaca gccgcaggaa
     8161 aaaggcaact aggtgcggat cggcaggcgg gcaggctgtc gagatcttcg agccggcgct
     8221 cgaaagcggc cggataagga ggagctggag gtcgtaatag aaatttctta ttcaatagtc
     8281 gagatcataa aattaccaga tgaccaacgc ggatcgatcc gcgcgcggcc tgttcaatgt
     8341 atgagcccgg caatatttcc tgaaatccag gcggactctt cacttgatgg aaatcaatga
     8401 agttttattg tgtcgctgaa tttaacccgt ttgggcgatg actgaagcca ccgctttgcc
     8461 tatagattcg atattcctaa aacaggagcc cgaatgagaa ttcttgataa tctgaccggc
     8521 ggggagcagc ctgccttcgc cagtaactgc aacggcagga tcgtcgactg gaaccagggc
     8581 gccgagcgct tgctcggtta ctcggccgcg acgatgatcg gcaggcattg tttcgaagta
     8641 ctcgacggca aggatgtttt cggcaaccgg ttttgtcacg agcgatgcgc catcaggtgc
     8701 atgattcgac gccacgagcc ggtacaccac tggcagttaa actaccggac cgcatcgtcc
     8761 gcaagaatcg acgtctcggt aggcgcagtc gtggtgaacg gcgatgaatc ggcagacagc
     8821 ctgatcgtgc acttgctgca gccggtcgac agtcgcggga atgaattcgg gccggcgatt
     8881 ccgctcaagc ccagcgaata ggcgcggcga ggcggtggac gcggattcca ggccggtgag
     8941 gctgaccgcc cggaagcacg agatacttcg cttgctggcc gccggctcgt cgacctcgga
     9001 gatagtccac cagctgagca tcaacgccga cacggtgcgc acccacatac gcaacatctt
     9061 ctacaagccc aatgcctaca cccggctgga ggccgtcggc aaggctttgc gcaggcgact
     9121 gctgtagaca ctctccccgg ctcgacctca gcgtaatcag gcgcccgatt gcgggaactc
     9181 t
//
LOCUS       DATCZV010000028         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_107885, whole genome shotgun sequence.
ACCESSION   DATCZV010000028 DATCZV010000000
VERSION     DATCZV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107885"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VIW27_00660"
     CDS             complement(<1..1479)
                     /locus_tag="VIW27_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5738198.1"
                     /translation="MQQNELAVTSDLVLVGGGHSHLFVLKYFAMNPVPGVRLTLVSRD
                     LHTPYSGMLPGFIAGHYKFDDAHIDLLPLARYAGARVIHDEVTGLDAERGRVQFANRP
                     ELDYDLLSINIGSRPTSPPLAQTDDLQFAVKPVDRFIESWERLERRLLDSEQDFKLAV
                     IGGGAAGVELALSLDYRARQLQAPSARLELRLVTDRPQLLPGHNARVRRMFGRILEQR
                     AIPVHYDFGVSCFEDGLLRSNHAATIAADAAIWVTQASPAGWLQESGLKLDDRGFIAV
                     TSGLQSVSHDNLFAAGDIASVGEFPRPKSGVFAVRQGLPLARNLQRWLRGRRPRPFKP
                     QRQFLSLISTGDRFAVASRGPWALQGNWCWWLKDRIDRKFVRRFTELPPMQAASDESD
                     DLAPMRCGGCGSKVGSEILDQVLAEIGSRYAATRGSGFEHADDAALIRVPAGLELVQS
                     VDHFRSFIDDPYLFGRIAANHALGDLFAMGVDPHSALVIANVV"
     gene            1563..>2240
                     /locus_tag="VIW27_00665"
     CDS             1563..>2240
                     /locus_tag="VIW27_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5738199.1"
                     /translation="MFKLFRGPARWAGLLIFALAATPLAALQFPEVDQGYPGVRVEQL
                     ATGLGIPWGMTFLSPRQMLITERGGSIRLLDIDSGDVTALQGAPRVVAEGQGGLLDVA
                     AGPDFANDGWIYFTFSKPVDSEAATTLARARLRENRLVDWQELLVTDSATGTRRHFGS
                     RIAFDDQGHVFFGVGDRGERDMAQNLGNHIGGIMRLRVDGSIPPDNPFVGDPQARDEI
                     WSYGHRNP"
BASE COUNT          441 a          715 c          688 g          396 t
ORIGIN      
        1 caccacgttg gcgatcacca gggcgctatg agggtcgacg cccatggcga acaggtctcc
       61 gagggcatgg ttggccgcga tacgtccgaa caggtacggg tcatcgatga aactgcgaaa
      121 atgatcgacc gactgcacca gctccaaccc ggccggcacc cgaatcaggg ctgcgtcgtc
      181 ggcatgctcg aaacccgagc cacgggtggc cgcgtagcga ctgcctatct cggctagcac
      241 ctggtcgagt atttcgctac cgaccttcga accacagccg ccgcaacgca tcggcgccag
      301 gtcgtcggac tcgtcgctgg ccgcctgcat cggcggcaac tcggtaaaac gacgcacgaa
      361 cttgcgatcg atcctgtctt tcaaccacca gcaccagttt ccctgtaatg cccaggggcc
      421 acggctggca acggcgaaac gatcgccggt ggaaatcagc gacagaaact gccgctgcgg
      481 tttaaatggc ctgggtcgcc gcccccgcag ccaacgctgc aggtttcgcg ccagcggcag
      541 gccctggcgc accgcgaaca cgcccgactt gggtcgaggg aattcaccga ccgaggcgat
      601 gtcgccggcg gcgaacaggt tgtcatggga aacgctttgc aaaccggaag tcaccgcgat
      661 aaaaccccga tcgtcgagct ttagcccgga ttcctgtaac caacccgccg ggctggcctg
      721 ggtaacccag atggcggcat cggcggcgat cgtggccgcg tgattgctgc gcaacaatcc
      781 atcctcgaaa caactgacac caaagtcgta atgcaccggg atagcgcgct gctcgaggat
      841 gcggccgaac attcgcctga cgcgagcgtt atgaccgggc agcagctggg gtcgatcggt
      901 aacgaggcgc agctcgagcc tggcgctcgg cgcttgcagc tggcgcgcgc ggtagtccag
      961 cgacagtgcc agttcgacgc ccgcggcacc gccgccgatg accgccagct taaaatcctg
     1021 ctcgctatct agcagccgcc gttctagccg ctcccaggat tctatgaatc gatcgaccgg
     1081 cttgaccgcg aactgcagat cgtcggtttg cgcaagcggc gggctcgtcg gccgcgagcc
     1141 gatgttgatc gacaacaggt catagtcgag ctcgggacga ttagcaaact gcacccggcc
     1201 acgctcggca tcgagcccgg tcacctcgtc gtgaatcacg cgagcaccag cgtagcgcgc
     1261 cagcggcagc aggtcgatat gggcatcgtc aaatttataa tgcccggcaa tgaaaccggg
     1321 cagcatcccc gaatacggtg tatgcagatc gcggctcacc agggtcagcc ggacgcccgg
     1381 caccgggttc atcgcgaaat acttgagcac gaacaaatga ctatgcccgc ccccgaccag
     1441 caccagatcg gaggtgaccg ctaattcatt ctgttgcatg gcacaaacgc gtcatgggag
     1501 ccctggttcc gggattgata gtggcactgg tattatgcgt ccagcttttg tactgtaaag
     1561 ccatgtttaa attattccgc ggcccggctc gatgggcggg tctcctgatt ttcgctctgg
     1621 cggcaacccc gctggcggca ctgcagttcc ccgaggtcga tcagggctat cccggcgtac
     1681 gggtcgagca actggcgaca ggactcggca ttccctgggg catgaccttt ttgtcgccgc
     1741 ggcaaatgct tattaccgaa cgtggcggca gcatccgcct gctggatatc gatagcgggg
     1801 acgttactgc gctgcagggt gcccccaggg tcgtcgccga gggccagggc ggcctgctgg
     1861 acgtcgcggc cggccccgat tttgccaacg acggctggat ttacttcacc ttcagcaaac
     1921 cggtcgattc cgaggcggca acgacgctgg cgcgagcgcg tttgcgggag aatcgcctgg
     1981 tcgactggca ggagctgctg gtgaccgatt ccgctaccgg tactcgtcgc catttcggca
     2041 gccgcatcgc cttcgacgat cagggtcacg tattcttcgg cgtgggcgat cgaggcgaac
     2101 gcgacatggc gcaaaacctc ggcaatcaca tcggcggcat catgcggctc agggtggatg
     2161 gcagcatacc gcccgacaat cctttcgtcg gcgatcctca ggcgcgcgac gaaatctgga
     2221 gttacggcca ccgcaatccc
//
LOCUS       DATCZV010000029         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_637197, whole genome shotgun sequence.
ACCESSION   DATCZV010000029 DATCZV010000000
VERSION     DATCZV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637197"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VIW27_00670"
     CDS             <1..1170
                     /locus_tag="VIW27_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY5738200.1"
                     /translation="PPLAGIPIALKDLFDVRNEVTLAGSVVLRHDARPAAADAAVVAP
                     LRDAGLLFLGRTNMTEFAFSGIGKNPHYGTPLSIWDRASGRLPGGSSSGSGVAVGDGI
                     VCAALGSDTAGSCRIPAAFNGVVGVKPSHGRLPLDGIYPLSPTLDAPGPLAVDVDSCF
                     VLDQLMGGYAAAAGELPRLEAAKLAESRFAVPRARVMEGLDDEVKIAFESALDRLAAA
                     GARIIEQPMTVLDDCDDLFIERPLVIYEVWQHHRQLLQAHGDEYDPFVGQRMSAGAGI
                     DAAEQGRRVDARNRLVAEFQRRFAALEVDALLYPTVACVPPAIADAEDDDDARRVNLR
                     CLRNTATVNYFDGCAISLPCHRPGSAPVGLMLSTVNGADESLYRLAAAVETALAS"
     gene            1305..1586
                     /locus_tag="VIW27_00675"
     CDS             1305..1586
                     /locus_tag="VIW27_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738201.1"
                     /translation="MTPNPSNKKSAGSTNKETTAMHCYQYIAAVSAVANTVESCCGAG
                     QKLPYSVLADWMEENGIEAEDVLLVAGSEVRAIVESLLRQDQPHLPHAA"
     gene            1912..3303
                     /gene="hemN"
                     /locus_tag="VIW27_00680"
     CDS             1912..3303
                     /gene="hemN"
                     /locus_tag="VIW27_00680"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY5738202.1"
                     /translation="MPTPPQFRKDLIERYDKPGPPYSSYPPASEFHDKITEADYRQWV
                     RQSNEELIPKPLSLSVHIPFCSSICHYCGCHTVIKGSKKKVEPYLHDLYREIELQGRL
                     FDADRAVRQLHWGGGTPGFLTRQQSERLMAELGRHFRLDPSGAGEHSIEVDPRVMQAG
                     DMRHLRGLGFNRISIGVQDFDPRVQQAVNRSQSIEETAAVIDEARDCGIRSINIDLIY
                     GLPHQGVNSFDTTLDTVIALDPDRIALYSYSHLPKSNPPQRRIRAEDLPLPAENLAIL
                     EAAIDRLCAAGYEYIGMDHFARPKDDLAMAQRRGMLVRNFHGYSTLCGCDSIGFGVSA
                     VSQVADNYSQNATDLKSYHSSLARYQLPVVRGYRSDHEDLLRRAVIQDLACRFRLDKR
                     RIASEWGIDFEHHFSDELDRLETLQQDGLVEIGDTAIRVLAPGRLLVRGICMIFDQFQ
                     QCRNYDVDLSRTL"
     gene            complement(3439..3627)
                     /gene="ccoS"
                     /locus_tag="VIW27_00685"
     CDS             complement(3439..3627)
                     /gene="ccoS"
                     /locus_tag="VIW27_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY5738203.1"
                     /translation="MEVIYLLIPVSLILLGGILWVLLWAVRDGQYDDLEGPAHRILMD
                     EDKVVDDSQKKSRREDVG"
     gene            complement(3641..>4361)
                     /locus_tag="VIW27_00690"
     CDS             complement(3641..>4361)
                     /locus_tag="VIW27_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5738204.1"
                     /translation="LRGEIGGREYAIGSADFVTAAAAVEVPPEWLQSIAEQAASAVFL
                     ASRERVLAMFVFSDRLRDDAAAVIAALRARGLEVMLLTGDRAAAAAQAARACGIDEYR
                     AALSPDDKLRAVRGLQSTGARVMMIGDGINDAPVLAAADVSVAMGGATALARVSADIV
                     LMTDRLPAIAEVFEIAARTRRVVLQNLGWALLYNFGAIPAAALGLVAPWLAALGMSAS
                     SLVGVSNALRLGRRPTGRDTR"
BASE COUNT          801 a         1460 c         1346 g          754 t
ORIGIN      
        1 ccaccgctgg cgggcattcc gatcgcgctc aaggacctgt tcgacgtgcg caacgaagtc
       61 acactggcgg gctcggtggt gctgcgccac gacgcgcggc cggcggccgc cgacgccgcg
      121 gtggtggcac cgctgcgcga cgcgggcctg ctgttcctcg gccgcaccaa catgaccgag
      181 ttcgcgtttt ccggaatcgg caagaatccc cattacggta cgccgctgtc gatctgggat
      241 cgcgccagcg gccggctgcc gggcggttcg tcgtccggca gcggcgtcgc cgtcggcgat
      301 ggcatcgtct gcgccgcact cggctcggac actgccggtt cgtgccgcat tccggccgcc
      361 ttcaacggcg tagtcggcgt caagccgagt cacgggcgcc tgccgctgga cggcatttat
      421 ccactgtcgc cgaccctcga cgcaccggga ccgctggcgg tcgacgtcga cagttgcttc
      481 gtgcttgacc agctgatggg cgggtatgcc gccgcggccg gcgaattgcc gcggctcgag
      541 gcggcgaagc tcgccgagag ccggttcgcg gtgccgcgcg cacgggtcat ggaaggactc
      601 gacgacgagg taaagatcgc tttcgagtcg gcactcgatc gcctggcggc ggctggcgcg
      661 cgcatcatcg agcagccgat gacggtgctc gacgactgcg acgacctgtt catcgaacgg
      721 ccgctggtga tctatgaagt ctggcagcac caccggcaac tgttgcaggc gcatggcgat
      781 gagtacgatc cgttcgtcgg ccagcggatg tcggccggtg ccgggatcga tgccgcggaa
      841 cagggccggc gggtcgacgc gcgcaaccgg ctggtcgccg aattccagcg ccgtttcgca
      901 gcactcgaag tcgatgcgct gctctatccc acggtggcct gcgtgccgcc ggcaatcgcc
      961 gatgccgaag acgacgacga tgcgcgccgc gtcaacctgc gctgcctgcg taataccgct
     1021 accgtcaact atttcgacgg ctgcgcgatc agcctgccgt gtcaccggcc aggatcggcg
     1081 ccggtcggcc tgatgctgtc gacggtgaac ggcgccgacg aatccctgta tcgcctcgcc
     1141 gcggctgtcg agacagcact ggcgagctag cccgcatcat cgctcgccgc ttgcccgccg
     1201 caactgtcgc aatgcgcacc acgaaagcgc aatccgtgca ccattagcaa tcaaaacgtc
     1261 atctttggcg gttaaatttg catgccgttc acagcaacct aagattgacg ccgaacccgt
     1321 cgaacaagaa gtcggcggga agcactaaca aggagaccac cgccatgcat tgctaccagt
     1381 acatcgccgc cgtcagcgcg gtcgcaaaca ccgtcgaatc ctgctgtggc gcgggacaaa
     1441 agctgcccta cagcgtgctc gcggactgga tggaggaaaa cggcatcgag gccgaggacg
     1501 tgctgctggt cgccggcagc gaggtcaggg ccatcgtcga gagcctgctg cggcaggatc
     1561 agccgcacct gccgcacgcg gcctgagcgc gacagccgcg ggttcggccg ccagttgccg
     1621 aacccgccca ttttaagggg cggagtctcc cccgacgttc gcaccgccgt gatcccgggc
     1681 aagccccctc ggcccggccg agtgaggccc aacgggattt ttttaccccg cttatcgatc
     1741 accccggctc gcagtggcag aactgccaca gcgggctgtg tggcgccatt gggcacgcta
     1801 gccggcgggt cgaatgccac cagtacctca tcggcggcga gttgattcac atcaatgcat
     1861 atctgcggga atcaaatagc ctgtgagcat aattcgaatt cgatccagac agtgccgaca
     1921 ccgccccagt ttcgcaaaga ccttatcgag cgctacgaca aaccgggccc gccctatagc
     1981 tcctatccgc cggccagcga gttccacgac aagattaccg aagccgatta ccgccaatgg
     2041 gttcgccaaa gcaacgagga actgatcccg aagccactgt cgctgtcggt gcatattcca
     2101 ttctgcagtt cgatctgtca ctactgcggt tgccacacgg tgatcaaggg cagtaagaag
     2161 aaggtcgaac cctacctcca cgacctgtac cgcgaaatcg aactccaggg gcgcctgttc
     2221 gatgccgacc gagcggtcag gcagctgcac tggggcggcg gcacacccgg atttctgact
     2281 cgccagcaat cggagcgact gatggcggaa ctcggccgac attttcgcct cgacccgagc
     2341 ggcgccggcg agcactcgat cgaggtcgat ccgcgggtca tgcaagccgg cgacatgcgg
     2401 cacttgcgcg gcctgggttt caatcgcatc agcatcggtg tgcaggattt cgatcctcgc
     2461 gtgcaacagg ccgtgaatcg ctcgcagagc atagaggaga ccgccgcggt gatcgacgaa
     2521 gcgcgcgact gcggcattcg ctcgatcaac atcgacctga tttacggctt gccacatcag
     2581 ggtgtgaaca gcttcgacac caccctggat accgtgatcg cactcgatcc ggatcgtatc
     2641 gcgctctata gctacagcca cctgccgaaa agcaatccgc cgcagcgccg cattcgcgcc
     2701 gaggatctgc cgctgccggc ggaaaacctc gctattctcg aggccgctat cgacaggctt
     2761 tgcgctgcgg gctacgagta cattggcatg gatcacttcg cacgacccaa agacgacctg
     2821 gccatggccc agcgccgcgg catgctagtc aggaatttcc atgggtactc gacgctctgc
     2881 ggctgcgatt cgatcggttt cggcgtatcg gcggtcagcc aggtagcaga taactacagc
     2941 cagaacgcga ccgacctgaa gagttaccac tcaagcctcg cccgctatca gttgccggtg
     3001 gtgcggggct accgcagcga ccacgaggac ttgttgaggc gcgcggtcat tcaggatctg
     3061 gcctgccggt ttcggctcga taagcggcgc atcgccagcg agtggggtat cgatttcgag
     3121 caccatttca gcgacgaact cgaccggctc gagacattgc agcaagacgg actggtcgag
     3181 atcggcgata cggcgattcg cgtactggcg cccggccggc tgctggtgcg cggaatctgc
     3241 atgatcttcg accagttcca gcagtgccgc aattacgacg tcgacctctc gagaaccctg
     3301 taagcgtccc ctcgccgatg tttgcttgcc agcaataagg ccggggcaca agcgtcacct
     3361 attgatctac gtcaaagtct gggcggttta aatgggtagg attgtgcccc gactcctgtt
     3421 tcggtttcgc agagtctttt agccgacatc ctcccgtcgg cttttttttt ggctgtcgtc
     3481 gacgaccttg tcctcgtcca tcaggattcg atgcgccggt ccctcgaggt cgtcgtactg
     3541 gccgtcgcgc actgcccaca acaacaccca gaggataccg cccaacagga tcagcgatac
     3601 cgggatcagc aggtagatga cttccatgat tcggctccgt tcagcgcgtg tcgcggccgg
     3661 tgggccggcg gccgagccgc aatgcattcg atacccccac cagcgaactg gccgacatgc
     3721 ccagcgccgc cagccacggc gcgaccagtc ccagcgcggc ggccggaatc gcgccgaaat
     3781 tatacagtaa tgcccagccg aggttctgca gcacgacacg ccgcgtacgc gccgcgatct
     3841 cgaagacttc ggcgatcgct ggcaagcgat cggtcatcag cacgatgtcg gcactgaccc
     3901 gcgcgagtgc ggtggccccg cccatcgcca ccgacacatc ggcagcggcc agcaccggtg
     3961 catcgttgat gccgtcaccg atcatcatca cccgcgcgcc ggtgctttgc agcccgcgta
     4021 ccgcgcgtaa cttgtcgtcc ggcgacagcg cggcgcggta ttcgtcgatt ccgcaggccc
     4081 tcgcggcctg cgccgcggcc gccgcccggt caccggtcag cagcatgacc tcgaggccgc
     4141 gcgcgcgtaa cgcggcgatc accgccgcgg catcgtcgcg caaacggtcg ctgaacacga
     4201 acatcgccag cactcgctcg cggctcgcca ggaacaccgc gctcgcggcc tgttcggcaa
     4261 tcgactgcag ccattcgggt ggaacctcga ccgcggcggc ggcagtgacg aagtcggcac
     4321 tgccgatggc gtattcgcga ccaccgatct cgccacgcag g
//
LOCUS       DATCZV010000030         8269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_55387, whole genome shotgun sequence.
ACCESSION   DATCZV010000030 DATCZV010000000
VERSION     DATCZV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55387"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VIW27_00695"
     CDS             <1..328
                     /locus_tag="VIW27_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962790.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEY5738205.1"
                     /translation="CVIAHGAIVRIRARNPQLADQLASNYRKADITPRQLAMLDFAVK
                     IAKRADAIDDKDFARLREAGFDDDDIWDIGAISAFFGLSNRLANMMSLRPNDEFYLMG
                     RVPREK"
     gene            368..838
                     /gene="phaR"
                     /locus_tag="VIW27_00700"
     CDS             368..838
                     /gene="phaR"
                     /locus_tag="VIW27_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY5738206.1"
                     /translation="MSEAETPQLRVIKKYPNRRLYDTEKSQYVTLQQIRELVLEEVPF
                     VVVDQKTDEDITRSILLQIIFEQESETNPLFSNDNLERFIRFYNAGAHLGFSDFIGQG
                     MTFFQQQQREFSKAMEGMASHNPMTFWTEMTQKNIDTWRQMMGLGPESDDSKKK"
     gene            979..1719
                     /gene="phbB"
                     /locus_tag="VIW27_00705"
     CDS             979..1719
                     /gene="phbB"
                     /locus_tag="VIW27_00705"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY5738207.1"
                     /translation="MQKIALVTGGTGGIGTAICIALADQGRKVIAGYYPPEKEQAYAW
                     KQKHRADGYDFEIVACDVSDYVSSENMLRDINNTIGAVDILVNCAGITRDKTLRRMTP
                     EQWDAVMRTNLDSAFNVTRHVVEGMSERGFGRIVNISSVNGQKGQFGQANYSAAKAGL
                     HGFTKAVAQELAARGVTVNSVSPGYVKTEMTDAIPEDIRNAIIQQVPMGRMATPDEIA
                     YVVSFLTDDRNTYMTGANLPVNGGMYMS"
     gene            1890..2993
                     /locus_tag="VIW27_00710"
     CDS             1890..2993
                     /locus_tag="VIW27_00710"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEY5738208.1"
                     /translation="MSEKYSWADQWFKAQQQFVDAWTDMAKSASVDKSSQTDLWSRGF
                     DMWRQALGGQGQPDAELAMRKCMDMGREYFAMAEQVGKGIADGGKPTEAINQWLEQLK
                     QALQQFSGMPSFNGANVNDFMKQWFTPTQSWQQMVAALAPMQQATWQMPGMNTSVFNM
                     GEAIDPMGKVLSAPGIGYFREPQEKQQKGMQLALEYNEANFKFNQAFLRVALESIQGF
                     QQKLMQIGPESMPKSLRQLYDLWVEVSEEHYAEFAMSDEYQTLYGDMVNRLMVMKKHY
                     HDIAAAGAGAAAEHARGRYHAAAPAPAAARKYRVEEGNSRDSRNTQASRFRNRAQAAR
                     ESAGRGGEKGGGKKSAGEEGRGESVGGEARRDG"
     gene            3101..4168
                     /locus_tag="VIW27_00715"
     CDS             3101..4168
                     /locus_tag="VIW27_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEY5738209.1"
                     /translation="MTNVPFRSDQLVDELVQFNQNLSKGVKNLLEAEEISEGVTPREE
                     VYAEDKLRLFRYQPSTGVVQNKIPTLIVYALVNRPYMADIQENRSTIKGLLDTGQDVY
                     LIDWGYPDRADKYLTLDDYINGYIDRCVDVMRERHGVDKVNILGICQGGVFSLCYTTL
                     HQDKVRNLVTMVTPVDCKTPDNMLSHWVQQMDIDAIVDAMGNLPGEMLNWTFLNLKPF
                     QLMGQKYVSMVDIISDEKNVKNFMRMEKWIFDSPDQAGEAFRQFIKDFYQQNKLVEGG
                     LKIGEHDVDLKNLRTPVLNIYAEQDHLVPPAASKILPKLIGSKDYTELSFPGGHIGIY
                     VSGKAQSTVTPAISEWLNKRS"
     gene            4417..4830
                     /locus_tag="VIW27_00720"
     CDS             4417..4830
                     /locus_tag="VIW27_00720"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5738210.1"
                     /translation="MSKVSVDNFNRILTAELPSAAENGIYARAIEAGKAELVLPYSER
                     SLRPGGTIAGPFMMLLADVCMYAVVLSLLGEIKLAVTTSLNINFLRKPGQGELVGRGS
                     SIKLGKRLAVIEVSIYSEDEIVAHATGTYSIPPQA"
     gene            5109..5894
                     /locus_tag="VIW27_00725"
     CDS             5109..5894
                     /locus_tag="VIW27_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5738211.1"
                     /translation="MDSPASANGAAPRVGLFVTCLVDLFRPSVGFAAVKLLEQAGCRV
                     EVPAAQTCCGQPAFNSGDDRHARELALQVLEAFEEFDYVVGPSGSCLGTIRCHYPEMF
                     AGDAALLPRFEALAANSHELVSFLVDVRGVDSVEAGYRHRVTYHDSCSSLRELGVREQ
                     PRKLLAGVAGLQLEEMQGTDICCGFGGTFCVKYPEISARMAADKIAAVEASGADTLLA
                     GDLGCLLNLAGKLRRDGRNTRVFHVAEVLAGMADGKAIGEGED"
     gene            5894..7315
                     /locus_tag="VIW27_00730"
     CDS             5894..7315
                     /locus_tag="VIW27_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEY5738212.1"
                     /translation="MRPDSQHFVANAEAALREPNLQAALKQLELGFVAGRRRCYDALP
                     EFEALRERAREVLDHTLENLDAYLLEYEARVTESGGRVHWARTPAEARDIVVGICRDA
                     EARGVIRGKSMIGEEIGVNEALEAAGFEVTETDLGEYILQIGKQPPSHIVAPAIHMTK
                     DEISDLFVEHHRELGFSARLTERNALVAEARAVLRRKFLAADVGMTGANFLIAETGSN
                     VIVTNEGNGDLSSTLPRVQIVTAAIDKVVPTLEDASLLLRLLPRSATGQEITAYTTFT
                     TGPRHDGDADGPDEYHVVLIDNHRSEMLASELRPMLRCIRCGACMNHCPVYGRIGGHA
                     YGWVYPGPMGSVLTPVMVGLDEAGHLPNACTLNGRCETVCPVKIPLPTLLRKLRSRQH
                     LERHNSWFARSALSLWTWVARRPRLYQALCRPAIALLALAGRKRGAFRSLPLASGWTR
                     ARDLPAPQGSTFQALWQRQRGRK"
     gene            7312..7962
                     /locus_tag="VIW27_00735"
     CDS             7312..7962
                     /locus_tag="VIW27_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY5738213.1"
                     /translation="MSGARERILAAVRAATGGSEADSSRLVSVRERIARHPRHIEPAI
                     GEPARQRFQAGLETAQASFAAVATLDEVGAEIERYLDSKNLARELVLGDDEVLRGMAW
                     PGSLRALPWEPGMRYEVSLARAFCGIAETGTLLLVSGANNPTEINFLPDYHIALLEAE
                     NLVPRMEDAWDRVRASFGKLPRTVNFISGPSKTADIEQTVEYGAHGPRCLHVILVG"
BASE COUNT         1712 a         2652 c         2519 g         1386 t
ORIGIN      
        1 ctgcgtcatt gcgcacggtg cgattgtgcg gattcgcgcc aggaatcccc agctcgccga
       61 ccagttggcg agcaattacc gcaaggccga catcacgccg cgccagctgg ccatgcttga
      121 cttcgcggtg aagatcgcga agcgggccga cgcaatcgac gacaaggact tcgcgcggct
      181 gcgcgaagct gggttcgacg acgacgatat ctgggatatc ggcgcgatca gcgccttttt
      241 cggcctttcc aaccggctcg ccaacatgat gagcctgcgc cccaacgacg agttttacct
      301 gatggggcga gtaccccgtg aaaaataggt tacacttgac gctcggcaga cagtcaggaa
      361 acaccaagtg agcgaagcag aaacaccaca actccgggtg atcaagaaat accccaaccg
      421 gcggctctac gataccgaga aaagccagta cgtgacgctg cagcagattc gcgaactggt
      481 gctcgaggag gtgccgttcg tggtcgtcga ccagaagacc gacgaagaca ttacccgcag
      541 cattctgctg caaatcatct tcgagcagga atcggaaacc aatccgctgt tttccaacga
      601 caatctggaa cgcttcattc gcttttacaa cgccggcgcc cacctcggat tttccgattt
      661 catcggccag ggcatgactt tcttccagca acagcagcgc gaattcagca aggcgatgga
      721 gggtatggcg agccacaatc cgatgacttt ctggaccgaa atgacgcaga aaaacatcga
      781 tacctggcgg caaatgatgg gcctgggccc ggaaagcgac gattctaaaa aaaaataacc
      841 ttctgattta aaagcctttt ctaaaaatca accgcggttt gcttgacacc cgcgagaatc
      901 cggcctattt ttgtgcagtg caaaaattgc tgcattgcag caggcatcct accaggtaaa
      961 tgtgatacga atatgaacat gcaaaaaata gcgctggtta ccggcggcac gggtggcata
     1021 ggcaccgcga tctgtatcgc gctggcggac cagggacgca aggtcatcgc cggctactac
     1081 cctcccgaga aggaacaggc ctacgcctgg aagcaaaaac accgcgccga cggctacgat
     1141 ttcgaaatcg tcgcctgcga cgtcagcgat tacgtatcca gcgaaaatat gcttcgcgat
     1201 atcaacaaca ccatcggtgc agtcgacatc ctcgtcaact gcgccggcat cacccgcgac
     1261 aagacgttgc gccgcatgac gccggagcag tgggacgcgg tcatgcgcac caacctcgat
     1321 tcggctttca acgttacccg ccacgtggtc gagggcatgt cggagcgcgg cttcggccgc
     1381 atcgtcaaca tctcttcggt caacggccag aaagggcagt tcggccaggc caactactcc
     1441 gcggccaagg ctggcctgca cggcttcacc aaggccgtgg cgcaggaact cgccgcccgc
     1501 ggcgtgaccg tcaactcggt atcgccgggc tacgtcaaga ccgagatgac cgacgcgatt
     1561 cccgaagaca ttcgcaacgc gatcattcag caggtgccga tgggccgcat ggccacgccc
     1621 gacgaaatcg cctacgtggt ctcgttcctg accgacgacc gcaacaccta catgaccggc
     1681 gccaacctgc cggtcaacgg cggcatgtac atgagttgat tcccggcccc gggaatcatt
     1741 ttccagatca cgcgctgcgc cttccgtgag gaaggcgcgt ggcggcgtgc gtttagccgc
     1801 ctgcacatat tcagattgcg catttatgtt gcactgcaca ataatctgtg ctaatgtaat
     1861 ttctttataa attttaacga gttatcgaca tgagcgagaa atattcctgg gccgatcaat
     1921 ggttcaaggc gcaacagcaa ttcgtcgacg cgtggaccga tatggcgaaa tccgcgagcg
     1981 tcgacaaatc cagccaaacc gacctgtggt cgcgcggatt tgatatgtgg cgccaggcgc
     2041 tcggcggcca gggtcagccc gacgcggaac tcgcgatgcg caaatgcatg gacatgggac
     2101 gcgaatactt cgcgatggcc gaacaggtcg gcaagggtat cgccgacggc ggcaagccga
     2161 ccgaagccat caaccagtgg ctggagcagc tcaagcaggc gctgcagcag ttcagcggca
     2221 tgccgagctt caacggcgcc aatgtcaacg acttcatgaa gcagtggttt actccgaccc
     2281 agtcctggca gcaaatggtc gcggcgctgg caccgatgca acaggcaacc tggcagatgc
     2341 ccggcatgaa cacgtcggtt ttcaacatgg gcgaagccat cgacccgatg ggcaaggtgc
     2401 tgtcggcacc cggcatcgga tactttcgcg aaccgcagga aaaacagcaa aaaggcatgc
     2461 agctggccct cgagtacaac gaggccaatt tcaagttcaa ccaggcgttc ctgcgtgtcg
     2521 cgctcgaatc gatccagggt ttccagcaaa agctgatgca aatcggcccc gaatcgatgc
     2581 ccaaatccct gcgccagctc tacgacctgt gggtcgaggt cagcgaggag cactacgcgg
     2641 aattcgcgat gagtgacgag taccagacgc tctacggcga catggtcaac cgtctgatgg
     2701 tgatgaagaa gcattatcac gacatcgccg cagctggtgc aggcgctgct gccgaacacg
     2761 cgcgaggtcg ataccatgca gcagcgcctg caccagctgc ggcgcgaaaa tatcgagttg
     2821 aagaaggaaa ttcgcgagat tcgcgaaata ctcaagcgtc ccgcttccgc aaccgtgctc
     2881 aagccgcgcg cgaaagcgcc ggccgtggcg gcgaaaaagg cggcggcaaa aaaagcgccg
     2941 gtgaagaagg ccgcggtgaa agcgtcggcg gcgaagcccg ccgcgacggc taaacctgaa
     3001 tcgacggcta aacctgaatc gacggcaaag cccgaatcga cggcgaagtc cgcgccgacg
     3061 gcgaagaccg aaaccgaatc caaagctgga gcctgatacc atgaccaacg ttccgtttcg
     3121 ttccgaccag ctggtcgatg aactggtgca gttcaatcaa aacctgagca agggcgtcaa
     3181 gaacctgctc gaggctgaag agatctccga gggcgtgacg ccgcgcgagg aagtctacgc
     3241 tgaggacaag ctcaggcttt tccgctacca accctccacc ggcgtggtac agaacaagat
     3301 cccgacgctg atcgtctacg cgctggtcaa ccgcccctac atggccgaca tccaggaaaa
     3361 ccgctcgact atcaagggct tgctcgatac cggccaggac gtttatctga tcgactgggg
     3421 ctacccggac cgcgccgaca agtacctgac gctcgacgac tacatcaacg gctacatcga
     3481 ccgttgcgtc gacgtgatgc gcgagcgcca cggcgttgac aaggtcaata ttctcggcat
     3541 ttgccagggc ggcgtgttca gcctgtgcta caccacgcta caccaggaca aggtcaggaa
     3601 cctggtaacc atggtgacgc cggtcgactg caagacgccc gacaacatgc tgagccactg
     3661 ggtgcagcaa atggatatcg acgcaatcgt cgacgcgatg ggtaacctgc ccggggaaat
     3721 gctcaactgg accttcctaa acctgaagcc gttccagctg atgggccaga aatacgtttc
     3781 catggtcgac atcatcagcg acgaaaagaa cgtcaagaac ttcatgcgca tggagaagtg
     3841 gatcttcgac agccccgacc aggccggcga agcgtttcgc cagttcatca aggacttcta
     3901 ccagcagaac aagctggtcg aaggcggtct gaagattggc gagcacgacg tcgacctgaa
     3961 gaacctgagg actccggtgc tgaatatcta cgccgagcag gaccacctgg tgccgccggc
     4021 ggcgtcgaag atcctgccga aattgatcgg cagcaaggat tacaccgagc tgtcattccc
     4081 gggcggacac atcggcatct acgtcagcgg caaggcgcag tcgacggtta cgccggccat
     4141 ctcggagtgg ctcaacaagc gctcctgaat gtcggcatca ccgaccccgc cctgcgggcg
     4201 gggtcgattg catggctggc taaatacagc gccattcgac cggcaaggct gcaatcggtc
     4261 cgtctgatcg acatgccggc cgagccatcc cgttgctgcc agcggtattc cgctgtttca
     4321 agccgtcccc gcgcaagcgg ggatctggta attaccttcg gccacggact ttgccgcttg
     4381 caaccgccgg ccgcgcattg ttaaatgcgc cgcgagatga gcaaggtcag cgtcgacaac
     4441 ttcaatcgaa tcctcaccgc cgaattaccc tccgcggcgg aaaacggtat ctacgcccgc
     4501 gccatcgagg ccggtaaggc cgaactggtg ctgccctaca gcgagcgctc gctgcgtccc
     4561 ggcggtacca tcgccggccc gttcatgatg ctgctcgccg atgtctgcat gtatgcggtg
     4621 gtgctgagcc tgctcggcga gatcaaactc gcggtaacca ccagcctgaa catcaatttc
     4681 ctgcgcaagc cgggccaggg cgaactggtc ggccgcggca gcagcatcaa gctcggcaag
     4741 cggctggcgg tgatcgaggt atcgatctac agcgaggacg aaatcgtcgc ccacgccacc
     4801 ggcacctact cgatcccgcc gcaggcctga taaaaaactg cttgcccagg aatgcgtcgg
     4861 gaagacgccg acagaagcgt gtcgcgaccc gagcgcccgg ggatggcccg ccttgcggag
     4921 caaggtggga gcgccttctg ccgttggctt tccggcgcat ccgtctcgca agggataatc
     4981 tcgtttcaga taatatcggg tttgacaccg acttcgacga cgggcccgta atcggcgccg
     5041 gctgacgcgg gcgtaaatcc gcgccgccgc ggcttctttt cacgcggcac tcggatacaa
     5101 tgcgcatcat ggactcaccc gcttccgcca acggagccgc gccgcgcgtc ggcctgttcg
     5161 tcacctgcct ggtcgacctg ttccgaccgt cggtgggttt tgccgcggtc aagctgctcg
     5221 agcaggccgg ttgccgcgtc gaggtgcccg cggcacagac ctgctgcggc cagccggcat
     5281 tcaacagcgg cgacgaccgg catgcgcgcg agctcgcgtt gcaggtgctc gaggcgttcg
     5341 aggagttcga ttatgtcgtc ggcccgtccg gctcctgcct gggcaccatt cgctgccatt
     5401 acccggaaat gttcgccggc gacgcggcac tgctgccgcg cttcgaggcg ctcgccgcga
     5461 acagccacga actggtctcc ttcctcgtcg acgtgcgcgg cgtcgactcg gtcgaggccg
     5521 gttaccggca tcgcgtcacc taccacgact cctgctcgtc gctgcgcgag ctcggcgtac
     5581 gcgagcagcc gcgcaaactg ctcgccgggg tagcgggact gcaactcgag gaaatgcagg
     5641 gcacggatat ctgctgcggt ttcggcggca ccttttgcgt caagtacccg gaaatttccg
     5701 cccgcatggc cgccgacaag atcgcggccg tcgaggccag cggcgccgac acgctgcttg
     5761 ccggtgacct cggctgcctg ctcaatctcg ccggcaagtt gcgccgcgac ggcagaaaca
     5821 cccgcgtctt tcacgtcgcc gaggtgctcg ccggcatggc cgacggcaag gccatcggcg
     5881 agggcgaaga ctgatgcggc ccgattcgca gcatttcgtc gccaatgccg aggcggcgct
     5941 gcgcgaaccc aacctgcagg cggcgctgaa acagctcgaa ctcggcttcg tcgccggccg
     6001 ccggcgctgt tacgacgccc tgcccgagtt cgaagcgctg cgcgaaaggg cgcgcgaggt
     6061 gctcgatcac acgctcgaaa acctcgacgc ctacctgctg gagtacgagg cgcgggtcac
     6121 cgaatccggc ggacgagtgc actgggcgcg aacgcccgcc gaggcgcgcg acatcgtggt
     6181 cggtatctgc cgcgacgccg aggcgcgcgg cgtgattcgt ggcaagtcga tgatcggcga
     6241 ggagatcggc gtcaacgagg cgctcgaggc ggccggcttc gaggtcaccg agaccgatct
     6301 cggcgaatac attctgcaga tcggcaagca gccgccgagc catatcgtcg cgccggcgat
     6361 tcacatgacc aaagacgaaa tttccgacct gttcgtcgaa caccaccgcg agctcggatt
     6421 cagcgcgcgg ctgactgagc gcaacgcgct ggtcgccgaa gcgcgcgcgg tgctgcgcag
     6481 gaaatttctc gccgccgacg tcggcatgac cggcgccaat ttcctgatcg ccgagaccgg
     6541 cagcaacgtc atcgtcacca atgagggcaa cggcgacctg tcgagcacgc tgccgcgggt
     6601 gcagatcgtc accgcggcga tcgacaaggt ggtgccgacg ctggaggacg cgtcgctgct
     6661 gctgcgcctg ctgccgcgca gcgccaccgg ccaggaaatc accgcctaca cgactttcac
     6721 caccgggccg cggcacgacg gcgatgccga cggccccgac gaataccacg tggtactgat
     6781 cgacaaccac cgcagcgaaa tgctggcctc ggagctgcgc ccgatgctgc gctgtattcg
     6841 ctgcggcgcg tgcatgaacc actgcccggt ctacggccgc atcggcgggc acgcctacgg
     6901 ctgggtctac ccgggaccga tgggctcggt gctgacgccg gtcatggtcg gcctcgacga
     6961 ggccgggcac ctgcccaacg cctgcacact caacggccgc tgcgaaaccg tctgcccggt
     7021 gaagatcccg ctgccgacac tgttgcgcaa gctgcgcagc cgccagcatc tcgagcgcca
     7081 caactcatgg ttcgcgcgca gcgcattgtc gctgtggacc tgggttgcgc gccggccacg
     7141 cctctaccag gcgctgtgcc gcccggcgat cgcgctgctg gcgctcgccg gcaggaagcg
     7201 cggcgcgttt cgcagcctgc cgctggcatc gggatggacc cgggcgcgcg acctgccggc
     7261 gccgcagggc tcgaccttcc aggcgctgtg gcagcgtcag cggggtcgta aatgagcggc
     7321 gcgcgcgagc gcatcctcgc cgcggtgcgc gccgcgaccg gcggcagcga agccgacagc
     7381 tcgcgactgg tatcggtgcg cgagcgcatc gcgcgccatc cgcgccatat cgagcccgcc
     7441 attggcgaac cggcgcggca acgattccag gcgggcctcg aaaccgcgca ggcgagtttc
     7501 gccgcggtcg ccacgctcga cgaggtcggc gccgagatcg agcgttacct cgattcgaaa
     7561 aacctcgcca gggagctggt tctcggcgac gacgaggtgt tgcggggaat ggcgtggccc
     7621 ggcagcctgc gcgccctgcc ctgggaaccc ggcatgcgct acgaggtgag cctcgcgcga
     7681 gccttttgcg gcatcgccga gaccggcacg ctgctgctgg tatccggcgc aaacaatccg
     7741 accgaaatca attttctgcc cgactatcac atcgccctgc tcgaggcgga aaacctcgtg
     7801 ccgcgcatgg aagatgcctg ggaccgcgtc cgcgcgtcgt tcggcaagct gccgcgcacg
     7861 gtcaatttca tatccggccc gtcaaagacc gccgacatcg agcaaaccgt cgagtacggc
     7921 gcacacggac cgcgctgcct gcacgtcatt ctcgtcggtt gacggctcaa cgggctgcgc
     7981 agcaataccg ccgctgccga acagccgttc gatgctgtaa tcggccattg caccctgccg
     8041 cacagacggg tggctcgatt catcacatgg agttcccgct tgctgcttac cgcctgattc
     8101 cagcgagaac ggactgcccg taaccaggcg aatatgaatc acgacctggc cggcccgaag
     8161 ccgccgcgga tcgttttgcg ggtgctgact atcggcatta gccggcggcg aagccgttgc
     8221 gtcgggtaat caggcggcga cggggaacgg ctcgtttccc gcccgggcc
//
LOCUS       DATCZV010000031         4758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_161245, whole genome shotgun sequence.
ACCESSION   DATCZV010000031 DATCZV010000000
VERSION     DATCZV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161245"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(130..266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            386..1954
                     /locus_tag="VIW27_00740"
     CDS             386..1954
                     /locus_tag="VIW27_00740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5738214.1"
                     /translation="MFRFKSRAWQLGLTTLMLVFSYLLGLTRPVAGFEYDLFLYILGL
                     QPSNGELVSIAEVRDAPQWSVVAVYASIFAFYVTRYTRSRASATSILTVFPLMFGLLM
                     LEVLLAVLLHVYLPIVFPALVMLLVSLALWSLDLYQRLSAGLLTRHRQVDLADVHECI
                     DRGDLKNAMILLKQCPLDDDLLEVGYELGMQLEARHHWASALYLYHWLSQYDPGLSDF
                     VTRIEEIREQRAAQLRRGQPPVEDERETQRIGNYELVERIAKGSTSVMYDARDTRTGK
                     RVALKLMALRQDRQNERARANSWLQEAVIVSRLEHENIVRIHDAEVLGDKAYIAMDYI
                     SGYSMSLRLRRREYLTVGECIRILKDMLRALSVAHGHGVIHGDIKPANIMYDIANDTY
                     ILTDFGAAYTGRRDRHSQQLIVGTPAYMSPEQLEGRKLDGRSDLFSLAVTLYHLLSGH
                     QPFAGDSLPELKRNIIAQEPDLNHLTLPAGITEVILKALQKKPYMRFADAQQMLTSVE
                     YCETQLYERMRQRS"
     gene            complement(1961..2875)
                     /locus_tag="VIW27_00745"
     CDS             complement(1961..2875)
                     /locus_tag="VIW27_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008226285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5738215.1"
                     /translation="MLPVPGRFARAVFYILLALLLFDLQAVIIKFMGERYAVQQLASF
                     RNVFGLIPSLLVLVLSREWHNRGRILLIRQWRLGLARGLSIAAAQFCFYLAITKMELA
                     TASTLTFIGPVFITLLSIPILGHRVGGWRLLAVLTGFGGVLLIMSPGSEVFTPYSLLP
                     LGAALGYSLSTVCVRLVDDDVPTPLINMYASIGALAGALGILFSTTGYQGVDSATDWL
                     LLLAMGMVGGCAVLSLITAYRLTKPSSLSPFEYFGIPFAFVLGWLFFGEAPFDTLIPG
                     VFLIVAGGLLIAWRERRKPTPPAPTTAD"
     gene            3091..4236
                     /gene="carA"
                     /locus_tag="VIW27_00750"
     CDS             3091..4236
                     /gene="carA"
                     /locus_tag="VIW27_00750"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5738216.1"
                     /translation="MPRPALLALQDGTLFHGSAIGADGIASGEVVFNTAMTGYQEILT
                     DPSYCQQLITLTYPHIGNTGVNSEDEEAERIHASGLIIRDLPLQDSNWRSEGGLQAYL
                     QDRGIVAIADIDTRRLTRLLRDRGAQAGAIIAGDGLSEEAAIEAARAFPGLAGADLAQ
                     VVSTTKPYDWTQTAWSLESGYRDNTDPRYHVVACDYGIKKNILCLLAERGARVTVLPA
                     RTPASEVLALKPDGIFLSNGPGDPEPCDYAIDAIAEILRTEVPVFGICLGHQLLALAS
                     GARTVKMKFGHHGANHPVQDLASKVVMITSQNHGFAVDEDSLPDCLRATHRSLFDGSL
                     QGIERSDRPAFSFQGHPEASPGPHDVAPLFDRFFELIAAYRSSNGAA"
     gene            4236..>4758
                     /gene="carB"
                     /locus_tag="VIW27_00755"
     CDS             4236..>4758
                     /gene="carB"
                     /locus_tag="VIW27_00755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024248.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY5738217.1"
                     /translation="MPKRNDIHSILIIGAGPIVIGQACEFDYSGAQACKALKEEGFRV
                     ILINSNPATIMTDPEMADATYIEPINWQTVEKIIEAERPDALLPTMGGQTALNCALDL
                     VRHGVLEKHGVQMIGATREAIDMAEDRELFRVAMSEIGLKTPKSFIAHSIEEAWQGQS
                     QIGFPVIVRPSFTM"
BASE COUNT          949 a         1495 c         1445 g          869 t
ORIGIN      
        1 actggcggca gccaggattg cgtaaacggg tttcatgtgg ttctcctgtt gagcaggaag
       61 acacacacag ttggcgacta tggaagattc ggcgacaagc tgtgagaatt accctccgta
      121 attctggtct tgttatgcaa accgccaggc cggtctccgg acttgcagac gccaaaagca
      181 tctgtttacc gttgcggggg cagtacccga tttgcacggg tttcccgatt ctcgtgttgc
      241 cacgcacctg gcgatgtccg ccgataatgg aaaatctgca ccaatttgtc cagtcgcctg
      301 tgctgccgat gtcgctgttg tataggattt gcgtcgttgc cgcatctgtc gcatgatcgc
      361 ggtgtcctat tttcgcgaac ttgccgtgtt tagattcaag tccagggcct ggcagctcgg
      421 tctgaccacg ctgatgctgg tattcagcta cctgctcggc ttgacccgcc cggtcgccgg
      481 gttcgagtac gacttgttcc tttatatact ggggctgcag ccatcgaacg gcgaactggt
      541 gtcgattgcc gaggtacgcg atgccccgca gtggagcgtg gtcgcggtct acgcatcaat
      601 ttttgccttt tacgtcacca ggtatacgcg ctcgcgggct tccgcgacct cgatcctgac
      661 cgtcttcccg ctgatgttcg ggctgctgat gctcgaagtc ctgctggcgg tgctgctgca
      721 cgtctacctg ccgatcgtat tcccggcgct ggtcatgctg ctggtttcgc tggctttgtg
      781 gtcgctcgac ctctaccagc gcctctccgc cggcctgctg acccggcatc ggcaggtcga
      841 cctggccgat gtgcacgaat gcatcgaccg cggcgacctg aaaaacgcga tgatcctgct
      901 caagcagtgc ccgctcgacg acgatctgct tgaagtcggc tacgaactcg gcatgcagct
      961 cgaggctcgt caccactggg cctcggcgct ttacctgtac cactggctgt cgcaatacga
     1021 tccgggtctc agcgatttcg ttacccgcat cgaggaaatt cgcgagcagc gcgcggccca
     1081 gctgcggcgc ggtcagccgc cggtcgagga cgagcgggaa acgcaacgga ttggcaacta
     1141 tgaactggtc gaaaggattg ccaagggatc gacgtcggta atgtacgatg cccgggatac
     1201 gcgcaccggc aagcgcgtcg cgttgaaact gatggccttg cgccaggacc gccaaaacga
     1261 acgcgcgcgg gctaacagct ggctgcagga agcggttatc gtgtcacggc tggagcacga
     1321 aaacatcgtc aggattcacg atgccgaggt cctcggcgac aaggcctata tcgcgatgga
     1381 ttacatttcc ggttactcga tgtcgctgcg cctgcggcgc cgcgaatacc tgaccgtcgg
     1441 cgagtgtatt cgaatattga aagacatgct gcgcgcgcta tcggtggccc acggccacgg
     1501 cgtcattcac ggcgacatca aacccgccaa tatcatgtac gacattgcca acgataccta
     1561 catcctgacc gatttcggtg ccgcctatac cggcaggcgc gatcggcatt cgcagcagct
     1621 aatcgtcggc acaccggcct acatgtcccc cgagcagctc gagggccgca agctcgacgg
     1681 ccgctccgac ctgttctcgc tcgcggtcac gctgtatcac ctgctgtcgg gccatcagcc
     1741 gttcgccggc gacagcctgc cggaactaaa gcggaatatc attgcccagg agcccgacct
     1801 gaatcacctg accctgccgg cgggcattac cgaggtcatc ctcaaggcac tgcagaagaa
     1861 gccttacatg cgattcgccg atgcccagca aatgctgacc tcggtggaat attgcgagac
     1921 gcaattgtac gagcgcatgc ggcagcgctc ctgaggggct tcaatcggcc gtcgtcggcg
     1981 ccggcggcgt gggtttgcgg cgttcgcgcc aggcgattaa caggccgccg gcgacgatca
     2041 ggaatacgcc cggaatcagg gtatcgaacg gcgcctcgcc aaagaacagc cagccgagca
     2101 cgaacgcgaa cggaatgccg aagtactcga acggcgacag gctggacggc ttcgtcagcc
     2161 ggtaggcggt gatcagcgac agcaccgcgc agccgcccac catacccatc gccagcaaca
     2221 gcagccagtc ggtggcgctg tcgaccccct ggtaaccggt ggtcgagaac aggatgccga
     2281 gcgcgccggc cagcgcgccg atcgacgcgt acatgttgat cagcggcgtt ggcacgtcgt
     2341 cgtcgaccag gcgcacgcag accgtcgaca gcgagtaacc gagcgcggcg ccgagcggca
     2401 gcaggctgta gggggtaaaa acctcgctgc cgggcgacat gatcagcaat accccgccga
     2461 atccggtgag tactgccagc agtcgccagc cgccgacccg atgccccaga atggggatcg
     2521 ataacagggt gatgaacacc gggccgataa aggtcagcgt cgatgccgtg gccagctcca
     2581 ttttggtgat cgccaggtaa aagcagaact gggcggccgc gatcgacagg ccgcgcgcca
     2641 gcccgagccg ccactgcctg atcagcagga tgcggccgcg gttgtgccat tcgcgggaca
     2701 aaaccagcac cagcaggctc ggaatcaggc cgaacacgtt gcggaaactg gccagttgct
     2761 gcaccgcgta gcgctcgccc atgaacttga tgatcaccgc ttgcagatcg aacagcagca
     2821 gcgcgagcag gatgtagaag accgctcgcg cgaagcggcc gggtacaggc aacatggtcg
     2881 aggccccgaa agacgaatga cggcgaacgc aatcgggcta atttagcaaa attcggccgc
     2941 gggcgtagac tcattcgtgc aatcggcata caattctcgt tcaagaaaat gctgctttct
     3001 gcagtgttgt cgcaaacatg atactgatgg gatgaacctg gggttcctcc catttttgtg
     3061 cgcgcgcatt tttttgaatt cggagagccc gtgccgagac ctgctctact cgccctgcaa
     3121 gatggaaccc tgtttcacgg tagtgctatc ggcgccgacg gcatcgcctc cggcgaggta
     3181 gtcttcaata ccgcgatgac cggctaccag gaaattctca ccgacccctc ctactgccag
     3241 cagttgatca ctctgactta cccgcacatc ggtaacaccg gcgtcaatag cgaagacgag
     3301 gaggcggagc ggattcacgc cagcggcctg atcattcgcg atctgccgct gcaggacagc
     3361 aactggcgca gcgagggcgg cctgcaggct tacctgcaag accgcgggat agtagcgatc
     3421 gccgatatcg atactcgccg cctgacccgg ctgttgcgcg accgcggcgc gcaggccggc
     3481 gcgatcatcg ccggcgacgg actttcggag gaggccgcga tcgaggccgc gcgcgcattt
     3541 ccgggcctcg cgggcgccga tctcgcgcag gtggtatcga ccaccaagcc ctacgactgg
     3601 acacaaaccg cctggtcact cgaatccggt tatcgcgaca acaccgatcc gcgctaccac
     3661 gtggtcgcct gcgattacgg catcaagaag aatattctgt gcctgctcgc cgagcgcggc
     3721 gcgcgggtta cggtactgcc agccagaacc ccggccagcg aagtactggc gctgaagccg
     3781 gacggcatct tcctgtccaa cggtcccggc gaccccgaac cctgcgacta cgcgatcgac
     3841 gcgatcgccg agatcctgcg caccgaggtg ccggtattcg gtatctgcct gggtcaccag
     3901 ttgctggcgc tggcgagcgg cgcgcggacc gtgaagatga aattcggtca tcacggcgcc
     3961 aatcacccgg tgcaggacct ggccagcaag gtggtcatga taaccagcca gaaccacggg
     4021 ttcgcggtcg acgaagacag tctgccggac tgcctgcggg ccacccaccg ctcgctgttc
     4081 gatggcagcc tgcagggaat cgagcgcagt gatcgtccgg cgttcagctt ccagggccac
     4141 ccggaagcca gccctggccc gcacgacgtc gcgccgctgt tcgatcgatt cttcgagttg
     4201 atcgcggcct accggtccag caacggagcc gcctgatgcc gaaaagaaac gatatccact
     4261 cgatcctgat catcggtgcc ggcccgatcg tcatcggcca ggcctgcgaa ttcgactatt
     4321 ccggcgccca ggcctgcaag gcgctgaagg aagagggttt tcgggtcatc ctgatcaatt
     4381 ccaatccggc caccatcatg accgatccgg agatggccga cgcgacctat atcgagccga
     4441 tcaactggca gacggtcgaa aagatcatcg aggccgagcg tcccgacgcg ctgttgccca
     4501 ccatgggcgg ccagaccgcg ctcaactgcg ccctcgacct ggtgcgccac ggggtgctcg
     4561 aaaagcacgg tgtgcaaatg atcggcgcta cccgcgaagc catcgacatg gccgaggacc
     4621 gcgaactgtt tcgcgtcgcg atgtcggaaa tcggtctgaa gacgcccaag tcgttcatcg
     4681 cccacagtat cgaggaagcc tggcaggggc agtcgcaaat cggctttccg gtgatcgtgc
     4741 ggccgtcgtt cacgatgg
//
LOCUS       DATCZV010000032         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_372962, whole genome shotgun sequence.
ACCESSION   DATCZV010000032 DATCZV010000000
VERSION     DATCZV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372962"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..483)
                     /locus_tag="VIW27_00760"
     tRNA            complement(407..483)
                     /locus_tag="VIW27_00760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(447..449),aa:Pro,seq:cgg)
     gene            complement(527..991)
                     /locus_tag="VIW27_00765"
     CDS             complement(527..991)
                     /locus_tag="VIW27_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5738218.1"
                     /translation="MIRIVLATVTLLLTMAGPAAKGELFKWVDEHGNTHYGDRPPRDA
                     KPETVSGEINSYESVVVEPLDPEPTDDPPAVDSKSVIMFVTSRCELCQQAREHFRANG
                     IPFDEHDIKKSETAAKAFKKLKGRAVPVILIGRKRMDGFHAETFDRIYNDKS"
     gene            complement(1028..1936)
                     /locus_tag="VIW27_00770"
     CDS             complement(1028..1936)
                     /locus_tag="VIW27_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5738219.1"
                     /translation="MPATALPRLAPLVFVLLWSTGFIAARYSMHSADPFVFLCLRFTL
                     TVLVLLPLLWLLDAPLPRGLWRYRHDMATGALLHCGYLGFVFWPIKNGVPTGIVAIII
                     GIQPIVTLALAALFIGERLDFRKLAGLAIGFIGVGVVIVGKYGPTLGAGGGVAPLDLG
                     MCVISLLSIAIGVFYQKKFCQRSQLLTGTLMQYVAGSLASAAFALSIGEVWRIDWTPT
                     FAAALAWQVLGLSIGAVLLLMYIIRNGEAGRISSMFYLVPPLVVLEAHFLFGESLGAV
                     SILGMLACIAGVTIVSRSPRPRNPLP"
     gene            complement(1975..>2508)
                     /locus_tag="VIW27_00775"
     CDS             complement(1975..>2508)
                     /locus_tag="VIW27_00775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5738220.1"
                     /translation="KQYGEVDYDLVLAGMRAQHGDQETLITLEEAGNAINALMEQQFA
                     EQTAANRKRGEEFLKSNAENEGVQVTESGLQYEVITEGDGAKPAVTDEVTVHYRGTLI
                     DGTEFDSSYSRGEPTSFKLDQVIPGWTEGVQLMNVGSKYRFVIPHDLAYGERGAGGAI
                     GPFETLIFEVELLDIKS"
BASE COUNT          481 a          761 c          787 g          479 t
ORIGIN      
        1 ccggcggggc ccgccacccc ggcgcacgct cctgcggaac gtacgccggg gccagcgctg
       61 cgcgctgtcc aaaatcgccc ccggcgattt tgtcgaacca atggggttgg ttccagatcc
      121 agcaaccaat cccaaaataa aatttttcgc ctgggccagg gcaaagggat tgggaagttt
      181 tgtcgttgag ggtgtctatg gtgactggat tgtgccggcc atccctggcc ggcaccccgt
      241 attacgcctc tgcgcggcgt aatacggggc cagcgctgcg cgctgtccaa attcgctccc
      301 ggcgaatttg tcgaaccaat ggggttggtt ccggatccag cgcccagccc caaaaaagaa
      361 aatgtcgcct caactgaagc gaaggcgaca ttttcttttt tgggtctggt cggggtgact
      421 ggattcgaac caacgacctc tgcctcccga aggcagcgct ctaccaggct gagctacacc
      481 ccgtgcgaag gggggctatt ctagggaaaa agccgccgat gctcaatcag gatttgtcgt
      541 tatagatgcg atcgaaggtt tcggcgtgaa aaccgtccat gcgcttgcgg ccgatcagga
      601 tcaccggcac cgcgcggccc ttgagtttct tgaaggcttt cgccgcggtt tccgattttt
      661 tgatgtcgtg ctcgtcgaag gggatgccgt tggcccgaaa gtgctctctc gcctgctggc
      721 acagctcgca gcggctggtg acgaacatga tgaccgattt actgtcgaca gccggcgggt
      781 cgtcggtcgg ctccgggtcg aggggttcga cgacaaccga ttcgtagctg ttgatctcgc
      841 cgctcaccgt ttccggcttc gcgtcccgcg gtggccggtc gccataatgg gtattgccgt
      901 gctcgtcgac ccatttgaac agctcgcctt ttgccgccgg accggccatc gtcagcagca
      961 gggttacggt ggcgagcact atcctgatca tatccgttcg aaaaaatccc gattatcggg
     1021 attatagtca cggtagcgga ttgcggggtc ttggcgagcg gctgacgatg gtgacaccgg
     1081 cgatgcaagc cagcataccg aggatcgaca ccgcaccgag ggattcgccg aacaggaaat
     1141 gggcctcgag caccaccagt ggcggtacca ggtaaaacat gctggagatg cggcccgcct
     1201 cgccattcct gatgatatac atcaacagca gcaccgcgcc gatcgacagt cccagcacct
     1261 gccaggcgag cgcggcggca aaggtgggtg tccagtcgat acgccagact tcgcctatcg
     1321 acaacgcgaa agcggcggag gccagcgaac cggcgacgta ctgcatcagt gtgcctgtca
     1381 gtaattgcga gcgctggcaa aacttctttt gatagaaaac gccgatggcg atcgacagca
     1441 gggagatcac gcacatgccg agatcgagcg gcgcgacacc gccgccggcg ccgagcgtcg
     1501 gcccgtactt gccgacgatg accacaccga cgccgatgaa gccgattgcc agtcccgcga
     1561 gcttgcggaa atcgaggcgc tcgccgatga acagcgccgc cagcgccagg gtcacgatcg
     1621 gttgaatgcc gatgatgatc gcgacgatac cggtcggcac gccgttcttg atcggccaga
     1681 agacgaagcc cagataaccg caatgcagca gcgcgccggt tgccatgtcg tgccggtagc
     1741 gccacaggcc gcgcggcagt ggtgcgtcga gcagccacag gaggggcagc agcaccagca
     1801 ccgtgagtgt gaaacgcagg cacaggaaca cgaacgggtc ggcgctgtgc atgctgtacc
     1861 tggcggcgat gaagccggtg ctccacagca gcacgaaaac cagtggagcg agccgcggca
     1921 gggccgtggc cggcattacc ggctagccct gccgcggctg gcgacgagac agtgtcagga
     1981 cttgatgtcc agcagctcga cttcgaagat cagggtttcg aacgggccga tcgcgccgcc
     2041 cgcgccgcgc tcgccgtagg cgagatcgtg cggtatgacg aaacggtact tgctgccgac
     2101 gttcatcagt tgcacgccct cggtccagcc gggaatcacc tggtcgagct tgaaactggt
     2161 cggttcgccg cgcgaatagg aactgtcgaa ttcggtgccg tcgatcagcg tgccgcgata
     2221 atggaccgtg acttcgtcgg tgaccgccgg ttttgcgccg tcgccctcgg tgatgacttc
     2281 gtactgcagg cccgattcgg taacctgtac accttcattc tcggcgttgc tcttgaggaa
     2341 ttcctcgccg cgcttgcggt tggcggcggt ttgctcggcg aactgctgct ccatcagcgc
     2401 attgatcgcg ttaccggctt cttcaagcgt aatcagggtt tcctggtcgc cgtgttgcgc
     2461 gcgcatgccg gccagcacca ggtcgtagtc cacctcgccg tactgctt
//
LOCUS       DATCZV010000033         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_267112, whole genome shotgun sequence.
ACCESSION   DATCZV010000033 DATCZV010000000
VERSION     DATCZV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267112"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VIW27_00780"
     CDS             complement(<1..473)
                     /locus_tag="VIW27_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738221.1"
                     /translation="MKQKLHRDLQALLKFYQQQIQNPPAEFTHATGVINKPEFFTLAD
                     LQRHLNNPLLRPEWVHVKLGGQRVELEQSCFTKTVQRRKLEFMDKQVLDDAIEQGAAV
                     VLEGIDILDSAVNDFVARLDESLPCVMANSVVFLSQAKNEAYDGHCDADDVLVVQL"
     gene            complement(530..2170)
                     /locus_tag="VIW27_00785"
     CDS             complement(530..2170)
                     /locus_tag="VIW27_00785"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY5738222.1"
                     /translation="MEDEYDYIVLGAGSAGCVLANRLSESGDFTVLIVEAGPLDDNFM
                     IHIPAGVHSVYKDPSINWNYHSEAEAGLEQRRIELPRGRVVGGSSSINAMVYMRGHPL
                     DYDRWNDELGLDGWSYADCLPYFRAGETSERGASEWRGDSGPLGVARAKLKNPLFDAF
                     LLAGESSGQGRSEDLNGFRPEGLARFDSTVRDGRRCSAATAHLKPALARANLTLVTGA
                     LVEQVLLNGRRANGVRLQYRGKSRIVRAAREVILSCGAINSPQVLMLSGIGPADELQR
                     HAIPVRHQLPGVGRNLQDHLKIDCNWRCTQPITYHRVTRPWQRLKIGMRWMLHHDGFG
                     ASNIWEAGGLIRGNERVAYPNLQYHFAPLAARYRGRRIALSQAFTLQVDQLRPLSRGR
                     ITLNSADPTDRPLVRFNYLAEQHDLRELVEGFRKMHELVMQPAFIAFRGERREPAPEV
                     KSDSEVSAWIRATASTDYHPSCSCAMGNGDDSVVDAEMRVHGIDGLRVVDASVMPEIV
                     SGNLNAPTQMIAARAADFILGKPQLAKEQASFHFQARA"
     gene            2284..>2906
                     /locus_tag="VIW27_00790"
     CDS             2284..>2906
                     /locus_tag="VIW27_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5738223.1"
                     /translation="MSEIYDFDQIVDRAGSGCVKWEKYAGRDILPMWIADSDFAVMPA
                     IQRALAERVAHPIYGYTQPSARLLELVAERMRQRYGWQVDADWVVPLPGMIGAFYLAC
                     RATGQAGDAIYLPSVIYPPFTRAAGLNDMVNQPLPMCWHDRRLRIDFDWLEQQLPRPG
                     QLLMFCNPQNPGGTVYRRDELERLADIVCRHQLVVCCDEIHSELVLDT"
BASE COUNT          482 a          922 c          952 g          550 t
ORIGIN      
        1 agctgcacca ccagcacgtc gtcggcgtcg caatggccgt cgtaggcttc attctttgcc
       61 tgcgacagga acaccacgct gttggccatc acgcagggca gtgactcgtc gaggcgggcg
      121 acgaaatcgt tgaccgcgga atcgaggatg tcgattccct caagcaccac cgccgcaccc
      181 tgctcgatgg cgtcgtcgag gacctgcttg tccatgaact cgagcttgcg tctctgtacc
      241 gtcttggtga agcagctctg ctccagctcg acgcgctgtc cgccgagttt tacgtgtacc
      301 cactccggcc gcagcaacgg gttgttgagg tgccgctgca ggtcggccag ggtgaaaaat
      361 tccggcttgt tgatgacccc ggtggcgtgg gtgaattccg ccggcggatt ctggatctgc
      421 tgctggtaaa acttgagcag tgcctggaga tcccggtgca gtttttgttt catgttgaaa
      481 acccgttcaa tactgtcttt cgatcaatgg ctgcgcacgc cgaccgcggt caggcccgcg
      541 cctgaaaatg aaagctcgcc tgttcttttg ccagctgcgg cttgccgaga atgaagtcgg
      601 ccgcgcgcgc ggcgatcatc tgcgtcggcg cgttgagatt gccgctgacg atttcaggca
      661 tcaccgaggc gtcgaccacg cgcaggccgt cgatgccgtg cacgcgcatt tcggcatcga
      721 ccaccgaatc gtcgccgttg cccatcgcgc agctgcagct cgggtggtaa tcggtggacg
      781 ccgtggccct tatccaggcg ctgacttcgc tgtccgactt gacctcgggt gccggttcgc
      841 ggcgttcgcc gcgaaacgcg atgaaggccg gttgcatgac cagttcgtgc atcttgcgaa
      901 agccctcgac gagttcgcgc aggtcgtgtt gctcggcgag atagttgaag cgcaccagcg
      961 ggcggtccgt ggggtcggcc gaattcagcg taatccggcc gcggctcaac gggcgcagct
     1021 ggtcgacctg cagcgtgaac gcctgcgaca gcgcgatcct gcggcctcgg tagcgggccg
     1081 ccagcggtgc gaagtggtac tgcaggttcg ggtaggcgac acgctcgttg ccgcgtatca
     1141 ggccgccggc ctcccagata ttggaggcgc cgaaaccgtc gtgatgcagc atccagcgca
     1201 tgccgatctt cagccgctgc cacgggcggg tgacgcgatg ataggttatt ggctgcgtac
     1261 agcgccagtt gcagtcgatc ttcaggtggt cctgcaggtt acggccgacc cccggcagtt
     1321 gatggcgcac cgggatcgcg tgccgctgca actcgtcggc cgggccgatg ccggacagca
     1381 tcagcacctg cggcgagttg attgcgccgc aactgagaat cacttcgcgc gcggcgcgca
     1441 cgatgcggga tttgccgcgg tactgaaggc gaacgccgtt ggcgcggcgg ccgttcagca
     1501 gcacctgctc gaccagtgcg ccggtgacca gtgtcaggtt ggcgcgcgcc agcgccggtt
     1561 tcaggtgggc ggtggcggcg ctgcagcggc gcccgtcgcg caccgtgctg tcgaagcgcg
     1621 ccagcccctc gggacgaaag ccgttgagat cctctgatct gccctggccc gagctttcac
     1681 cggccagcag gaaggcgtcg aatagcgggt ttttcagttt ggcgcgcgcc accccgagcg
     1741 ggccgctgtc gccgcgccac tcgctggcgc cgcgctccga ggtttcgccg gccctgaaat
     1801 acggcaggca atccgcgtaa ctccagccgt cgagcccgag ttcgtcgttc cagcggtcgt
     1861 agtccagcgg gtggccgcgc atgtagacca tcgcgttgat cgacgaggag ccgccgacca
     1921 cccggccccg cggcagctcg atacggcgct gttcgaggcc ggcctcggcc tcgctgtggt
     1981 aattccagtt gatcgacgga tccttgtaga ccgagtgcac cccggccgga atatggatca
     2041 tgaagttgtc gtccagcggc ccggcctcga cgatcaacac cgtaaagtcg ccgctctcgc
     2101 tgagccggtt ggcgagtacg cagccggccg agccggcgcc gagaacgatg taatcgtatt
     2161 cgtcttccat gcgcgcagca tcgcaagcga acccgggcag gtcaatccct gatccggcaa
     2221 aaggcttggt cgcggcacga gcatcgaata tgatcgttgg cgagcgcgag caagccaccg
     2281 aatatgagcg agatttatga cttcgaccag atcgtcgacc gtgccggcag cggttgcgtc
     2341 aaatgggaaa aatatgccgg ccgtgacata ttgccgatgt ggatcgcgga tagcgatttc
     2401 gcggtcatgc cggctattca gcgggcgctt gccgagcgcg tcgcgcatcc gatctacggt
     2461 tatacccagc catcggcacg gctgctggag ctggtggccg agcgcatgcg acagcgctac
     2521 ggctggcagg tggacgccga ctgggtagtg ccgttgccgg gcatgatcgg tgctttttac
     2581 ctggcctgcc gcgcgaccgg ccaggccggt gacgcgatct acctgccgtc ggtgatctac
     2641 ccgccgttca cgcgtgccgc cggtctcaac gacatggtca accagccgct acccatgtgc
     2701 tggcatgacc gccgcctgcg tatcgatttc gactggctcg agcagcagct gccgcggccg
     2761 gggcagctgc tgatgttctg caacccgcaa aacccgggcg gcacggttta tcggcgcgac
     2821 gaactcgagc ggctcgccga catcgtgtgc cgtcaccagc tggtcgtctg ctgcgacgaa
     2881 attcacagcg aactggtgct cgacac
//
LOCUS       DATCZV010000034         5946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_532013, whole genome shotgun sequence.
ACCESSION   DATCZV010000034 DATCZV010000000
VERSION     DATCZV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532013"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VIW27_00795"
     CDS             complement(<1..176)
                     /locus_tag="VIW27_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEY5738224.1"
                     /translation="MTEYRHLFQPLTLRHRRLDNRIVFGAHTANMSDLGLPGERHRAY
                     YEERAIGGAAMIVV"
     gene            271..1155
                     /locus_tag="VIW27_00800"
     CDS             271..1155
                     /locus_tag="VIW27_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738225.1"
                     /translation="MSRLDIDSMRALTMIAETGGITRAAKKLNLSQSAVSHKVKRLEQ
                     RLNRNLLARVDGKTGFTRDGEKLLAYARRMVRLHDEAWASFHQSDLAGELRLGITEDI
                     TAPDMARILSQFSSSFPNIALTSRVAHTPELVRWLERGEIDMALIEVFETHVLDTDFP
                     LGRQQVVWLLAEDFILEDGPIPYVTYHRECFYKNWAEQALARVDRSLRVVFECPSLEG
                     MVNAVQNGLGIGLVNYNVFEQRQSGKRVDGRGLTFERTLLPQPPPIQHVARFSRGIPS
                     KQMNKLLELIRQELKPVS"
     gene            1202..2440
                     /locus_tag="VIW27_00805"
     CDS             1202..2440
                     /locus_tag="VIW27_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY5738226.1"
                     /translation="MILKDIKTWVTVPPSGIGGAFWVIVKVTTDDGIEGIGECYGIPV
                     SGDIACRMVEDTFERYVAGEDPHHIETLFRRVYSAGFTQRPDVSMMGVFSGIEIAIWD
                     ILGKAHDLPVYQLLGGRFHDRIRTYTYLYPETLDSDGNPGRDAPDVYHDGDAAAARAL
                     DYVDMGFTAIKQDPAGPYGFQGGRELSLVELARCEDNLRKIREAVGDRADILFGTHGQ
                     MTTSSAIRLARRLEPYDPLWFEEPCPPDQMEAIGRVAASTRIPVATGERLTTRVEFHQ
                     ALKAGVAILQPDIGRSGGIWETKKIAVLAELFNAQIAPHIYCGPIAHAAAAHVAISCP
                     NFLILETIQTEFHDAILQRPLDWEAGYMPAPGEPGLGIVLNEAVIESHPYTSGGRLHL
                     EMCQAVLDSNNHKTITEIDT"
     gene            2437..3615
                     /locus_tag="VIW27_00810"
     CDS             2437..3615
                     /locus_tag="VIW27_00810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5738227.1"
                     /translation="MIGRPASLNLANWRQPPHSRWSFHHVREVVPSEPIYRGGQSAAL
                     PRDPVALDGLAVSGPGGENWSMERWRDASTTDALLVAHRGRLVHEWYADENIEDHPHI
                     VFSVSKSITAMLAGILVGRGLLDPAKPVVDYLPQLAGSGYRDATLQQVLDMTVRIAFE
                     EDYLATSGIYVEYRQATAWHPCEIEAIDSNLHDFLCRLERADGNHGEVWQYRSPNSDL
                     LGWILERVAGESVARLMSRELWQPMGAEKNGYITVDRKGGSRTAGGICVIPRDLLRFG
                     ELVRNRGEAMGRQVIPAGWIDDCSQGGSREAWRRGESAKEFVDGRYRNKWYQTGNAHQ
                     AMLAIGIHSQWIFIDPIAAVTIVKLSSQDLPLRPELDSVNLQMFENIAGFLEDSGATA
                     "
     gene            3634..4551
                     /locus_tag="VIW27_00815"
     CDS             3634..4551
                     /locus_tag="VIW27_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5738228.1"
                     /translation="MRSDLLPSLALAGGAALWGLYWIPVRGIEAAGVDALWTGPVIFG
                     AATLLFIPLVLTRLPSFARHWRHTLVPGLLAGCAFALYIASINLTDVVRAILLFYLSP
                     IWSTLLGIVMLNERLTANRVIGLLLAFCGLYVVLVVESGLPLPRNLGDWFALLSGICW
                     SLASVKLFQGGAQYMIEKVTLFVFFALLLSLLLLLWQQGSLDGMPGSGPLLGGWYWIA
                     LVAIAMLPITYLTIWPATLLSPARVGMLLMVEVIVGVASAAMLSGDPFGMRELAGAVL
                     IVSAAVIEVLRQQKLDDSGIVGARVPPPD"
     gene            4643..5335
                     /locus_tag="VIW27_00820"
     CDS             4643..5335
                     /locus_tag="VIW27_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY5738229.1"
                     /translation="MTSLVTNPLLLLAALLAIAAAGPAAADCNDRRAPGMDWSGCKKI
                     SKMLGDSDYTGSRFDDATLSLSQLDRSIFNNASLVKANLTRVDATASRFENADLTKAN
                     GYRAIFDEVVIKSSNLTKSEFFRASFRQAEIADVDWSKSELGRVDFDAARLANVDFSF
                     TNLSRVHFGHARLSNVDFEGAYTYLTRFEGVDLGAVKNLTQAQLDQACGDSRTRLQAG
                     LRASASWPCEEE"
     gene            complement(5455..>5946)
                     /locus_tag="VIW27_00825"
     CDS             complement(5455..>5946)
                     /locus_tag="VIW27_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5738230.1"
                     /translation="DNRILFGGRLGYFFGNNTDPAQDRTPRPYVRLVKSFYRTFPQLA
                     DEIRFAYAWSGPIGLTTRMAVHYQKYHGGDMVFAGGYSGFGVTASRFGARVGLAILDG
                     ADIPETRMRFAQTLPDYIPPEPFRWIGAQVTMYALDTLDERGGWRKLWIRMVQKMGFP
                     ITL"
BASE COUNT         1075 a         1859 c         1908 g         1104 t
ORIGIN      
        1 tcgacgacga tcatcgccgc gccgccgatg gcgcgctcct cgtagtaggc gcgatggcgc
       61 tcgccgggca ggccgagatc ggacatgttg gcggtgtgcg cgccgaacac gatgcggttg
      121 tcgagcctgc ggtggcgtaa cgtgagcggt tgaaaaaggt ggcggtattc ggtcatggcg
      181 gaagccctgt tttattgaaa acgaatatac cgattgtcga catatgaaca aaacgatata
      241 ttttcataaa atcaatcgaa tttcgtcata atgtctcgac tcgatatcga ctccatgcgc
      301 gcgctcacga tgatcgccga aaccggcggc attacgcgtg cggcgaagaa gctcaacctg
      361 tcgcaatccg ccgtcagcca caaggtcaaa cgcctcgagc agcggctcaa tcgcaacctg
      421 ctggcgcgcg tcgacggcaa gaccgggttc acccgcgacg gcgaaaagct gctcgcctac
      481 gcgcgccgca tggtgcggct gcacgacgag gcctgggcca gctttcacca gagcgacctc
      541 gccggcgagc tcaggctcgg catcaccgag gatatcaccg cgcccgacat ggcgcgtatc
      601 ctgtcgcagt tttccagcag ttttccaaac atcgcgctga cttcgcgcgt cgcgcatacg
      661 ccggagctgg tgcgctggct ggagcgcggc gagatcgaca tggcattgat cgaagtgttc
      721 gaaacccatg tactggacac cgatttcccg ctcggccgcc agcaggtggt gtggctgctg
      781 gcggaggatt tcatcctcga ggatggcccg attccgtacg tgacctatca ccgcgaatgc
      841 ttttacaaga actgggccga gcaggcgctg gcgcgcgtcg accgcagcct gcgcgtggtc
      901 ttcgagtgcc ccagcctcga gggcatggtc aacgcggtac agaacggcct cggcatcggc
      961 ctggtcaact acaacgtctt cgagcagcgc cagtcaggca agcgcgtcga tggccgcggc
     1021 ctgaccttcg agcgcacgct gttgccgcaa ccgccgccga tccagcacgt ggcgcgcttt
     1081 tcgcgcggca ttccgagcaa gcagatgaac aagctgctgg aactgattcg ccaggagttg
     1141 aaaccggttt cttaagccgc cggtttgcag taagttagca gttttaccac cggccgataa
     1201 catgattctg aaggacatca agacctgggt cacggtgccg cccagcggta tcggcggtgc
     1261 cttctgggtg atcgtcaagg tcaccaccga cgatggtatc gagggcatcg gcgagtgtta
     1321 cggcatcccg gtatcgggcg atatcgcctg tcgaatggtc gaggacacct tcgagcgcta
     1381 tgtcgccggc gaagacccgc atcatatcga aaccctgttt cgccgcgtct actcggccgg
     1441 tttcacgcag cgcccggacg tcagcatgat gggcgtgttc agcggcatcg agatcgctat
     1501 ctgggatatt cttggcaagg cgcatgatct gcccgtctac cagttgctcg gcggccgctt
     1561 tcacgatcgt atccgcacct atacgtatct ctacccggaa acgctcgaca gcgatggcaa
     1621 tcccggcagg gacgcccccg acgtatacca cgatggcgat gccgccgcgg cgcgcgcgct
     1681 cgactatgtc gacatgggct ttaccgcgat caagcaggat ccggccggac cctacggttt
     1741 ccagggcggc cgcgaactgt cgctcgtcga gctggcgcgc tgcgaggaca atctgcgcaa
     1801 gatccgcgaa gcggtcggcg atcgcgccga tatcctgttc ggtacccatg gacagatgac
     1861 cacctcgtcg gcgattcgcc tggcgcgccg tctcgaaccc tacgacccgc tgtggttcga
     1921 ggaaccctgc ccgcccgacc agatggaagc cattggccgc gtcgccgcgt cgacccgcat
     1981 cccggtagct accggcgagc ggctgacgac cagggtcgag ttccaccagg cgttgaaggc
     2041 cggcgtcgcc atcctgcagc ccgacatcgg gcgcagcggc ggtatctggg aaaccaaaaa
     2101 gatcgccgta ctcgccgaat tgttcaacgc gcagatcgcg ccgcacatct attgcgggcc
     2161 gatcgcccac gcggcggccg cgcacgtggc catcagttgc ccgaattttc tgatcctcga
     2221 aaccattcag accgagtttc acgacgcgat tctgcagcgc ccgctggact gggaagccgg
     2281 ttacatgccg gcgccgggcg agccgggcct cggcatcgtg ctcaacgagg cggtgatcga
     2341 atcgcatccc tacaccagcg gcggacggct gcatctcgaa atgtgtcagg ccgtgctgga
     2401 ttccaacaac cacaagacca ttactgaaat cgatacatga tcggacggcc cgcaagcctc
     2461 aacctggcca actggcgtca accgccgcac agccgctggt cttttcacca cgtgcgtgag
     2521 gtcgttccca gcgaaccgat ttaccgcggc ggccagagtg ccgcgctgcc gcgcgatccg
     2581 gtggcgctcg acgggctcgc ggtaagcggg ccaggtggcg aaaactggtc gatggagcga
     2641 tggcgcgacg cctcgactac cgatgcgctg ctggtcgcgc accgcggccg gctggtgcac
     2701 gaatggtatg ccgacgagaa tatcgaggat cacccgcata tcgtgttttc ggtcagcaag
     2761 tcgatcaccg cgatgctggc cgggatcctc gtcggccgcg gcttgctgga cccggcgaaa
     2821 ccggtcgtcg actacctgcc gcagctcgcc ggttcgggtt atcgcgacgc gacgctgcag
     2881 caggtgctcg acatgaccgt tcgcatcgcc ttcgaagaag actatctcgc gacctcgggt
     2941 atctatgtcg agtatcgcca ggcgaccgcc tggcatccct gcgagatcga ggccatcgac
     3001 agcaacctgc acgattttct ttgccgcctc gagcgcgccg acggcaatca cggtgaagtc
     3061 tggcaatatc gatcgccgaa ctcggacctg ctgggctgga tcctggagcg tgtcgccggg
     3121 gagagcgtgg cgcggctgat gagccgcgag ttgtggcagc cgatgggcgc ggaaaagaat
     3181 ggctatatca ccgtcgaccg caagggcggg tcgcgcaccg cgggtggcat ttgcgtgatt
     3241 ccgcgcgacc tgctgcgttt cggcgaactg gtgcgcaatc gcggcgaagc catggggcgg
     3301 caagtgatcc cggccggctg gatcgacgac tgcagccagg gcggcagccg cgaagcctgg
     3361 cggcgcggcg aatcggcaaa ggaatttgtc gacggaagat accgcaacaa gtggtaccag
     3421 accggcaatg cgcaccaggc aatgcttgcg atcggtatcc acagccagtg gatttttatc
     3481 gacccgatcg ctgcggtgac gatcgtcaag ctgtcatccc aggatctgcc gctgcgcccc
     3541 gaactcgaca gcgtcaattt gcagatgttc gaaaatattg ccggttttct cgaagattcg
     3601 ggggccacgg catgagccgc cgcgccggcg gcgatgcgta gcgacctgtt gccgagcctg
     3661 gcgcttgccg gcggcgccgc gctgtgggga ctgtactgga ttccggtgcg cggcatcgag
     3721 gcggccgggg tcgatgcgct gtggaccggt ccggtgatct tcggcgccgc gaccctgctg
     3781 ttcattccgc tggtgctgac tcgtcttccc agtttcgcca ggcactggcg ccatacgctg
     3841 gtgcccgggt tgctcgccgg ctgcgcgttc gcgttgtaca tcgccagcat caacctgacc
     3901 gacgtggtgc gcgcgattct gttgttctac ctgtcgccga tatggagcac gctgctcggc
     3961 atcgtgatgc tcaatgaacg cctgacggca aaccgggtga tcggcctgct gctggctttt
     4021 tgcgggctat atgtcgtgct ggtggtggag agcggcttgc cgctgccgcg caacctgggt
     4081 gactggttcg cgctgttgtc ggggatttgc tggtcgctcg cgtcggtaaa acttttccag
     4141 ggcggcgcgc aatatatgat cgaaaaggtc accctgttcg tattcttcgc attgctgttg
     4201 tctctgctgt tgctgctctg gcagcagggc agtctcgacg gcatgccggg tagcggacca
     4261 ctgctcggcg gctggtactg gatcgcgctg gtggcgatcg cgatgctgcc gatcacctac
     4321 ctgacgatct ggccggcgac gctgctgagc ccggcgcgcg tcggcatgct gctgatggtc
     4381 gaggtcatcg tcggcgtggc ttcggccgcg atgctctccg gcgatccctt cggcatgcgc
     4441 gaactggccg gtgcggtgct gattgtgtcg gccgcggtga tcgaggtgct gagacagcaa
     4501 aaactggacg attcgggtat agttggtgcc cgggttccgc ccccggacta gcactcgggc
     4561 gccgtcggca gccccggccg gcccggattt aatcaacgcg gttagtgcca agccgcgctg
     4621 cagcgaataa aggatcgatg caatgactag cttagtgacc aatcccctgc ttctgctggc
     4681 cgcgctgttg gcgatcgccg ccgccggtcc ggcagcggcc gattgcaatg atcgccgcgc
     4741 gcccggcatg gactggtcgg ggtgcaaaaa aatcagcaag atgctcggcg attccgatta
     4801 caccggcagc cgcttcgacg acgcgactct gtccctgagc cagctcgatc gcagcatctt
     4861 caacaacgcc agcctggtca aggcaaacct gacgcgcgtc gatgcgaccg ccagccgctt
     4921 cgagaatgcg gacctgacca aagcgaacgg ctaccgcgcg atctttgacg aagtcgtcat
     4981 caagagcagc aacctgacga agtcagaatt ctttcgcgcc agttttcgcc aggccgagat
     5041 cgccgacgtc gactggagca agtcggagct ggggcgggtc gatttcgatg ccgcgcggct
     5101 cgccaatgtc gatttcagct ttaccaacct gtcgcgagta catttcggcc acgcacgcct
     5161 gtcgaacgtc gatttcgagg gcgcctacac ctacctgaca cgtttcgagg gtgtcgacct
     5221 gggcgcggtg aagaacctga cccaggcgca gctcgaccag gcctgcggcg attcgcggac
     5281 gcgcctgcag gcgggcttgc gggcgagcgc atcctggccc tgcgaagagg agtgacagcc
     5341 ttgcctgcca tccggcaggc gcgccggatg tcgctgcgag tcggcaggga attgcgctag
     5401 aaaaaccgcc ggcttcgatg ccggcgcgac cccttgctgc tgcccgccga gcgctcagag
     5461 ggtgatcgga aaacccattt tctgcaccat gcgaatccac agcttgcgcc agccaccgcg
     5521 ctcgtcgagg gtatcgagcg cgtacatcgt gacctgggcg ccgatccagc gaaacggctc
     5581 gggtggaatg taatcgggca gggtctgcgc gaagcgcatg cgggtctcgg gaatgtctgc
     5641 gccgtcgagg atcgccagcc cgacgcgcgc gccgaagcgc gacgcggtca ccccgaaccc
     5701 cgagtaaccg ccggcgaaca ccatgtcgcc gccgtggtac ttctggtagt gcaccgccat
     5761 gcgcgtggtc aggccgatcg ggccgctcca ggcatacgcg aaacggatct cgtcggcaag
     5821 ctgcggaaag gtgcggtaga aggacttgac gagccgaacg taaggccgtg gggtcctgtc
     5881 ctgcgccggg tcggtattgt tgccgaagaa atagccgagc cgaccgccaa acaggatgcg
     5941 gttatc
//
LOCUS       DATCZV010000035         4057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_373250, whole genome shotgun sequence.
ACCESSION   DATCZV010000035 DATCZV010000000
VERSION     DATCZV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373250"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VIW27_00830"
     CDS             complement(<1..1115)
                     /locus_tag="VIW27_00830"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY5738231.1"
                     /translation="MSRDGELYVGLMSGTSLDGVDVAIVDFGQFPPRILYCATDAYPA
                     ELRAALQQLCRTQMTSLDALYTLDARLGDVYADSVERALQAANIDPQQVTALGCHGQT
                     LRHSPDSDPPYSVQIGDPNRLAARCGITTVADFRGKDIALGGQAAPLAPAYHRFLFHS
                     AEEDRAVVNIGGIANITWLPAKRDATPLGFDTGPGNTLLNYWSEKHRGAAYDDDGAWA
                     RGGRVDDALLEKILAGEPYFRAAAPKSTGTEYFSADWLENRLPASFEAQDIQATLTEL
                     TAATIAAALASLPGAAANCFVCGGGAHNGYLLERLRDRLAGCGLQTTDALGTNPDYVE
                     AAAFAWLARERINRRAGNIPAITNASRETLLGAVYAA"
     gene            complement(1112..2500)
                     /locus_tag="VIW27_00835"
     CDS             complement(1112..2500)
                     /locus_tag="VIW27_00835"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5738232.1"
                     /translation="MSFQDLDFKTERVCLSRSVNPLPSPADFHPGVLLKLLLVATVIM
                     LLGFSVSHWQPSAGAADSTPKSARLHYSLELPEPDLSLQRDIQRAAAEIDQVPWETVI
                     VEPGDTLASIFAGKGIPSTTTHRVARLNEQTRRLRYIKPGQEIRLLLDADRDLRQMKF
                     SPDVTSTLVIQRTEDQGFSSQVINYQLEAYPVFREGVIESSLFEAAAAADIPEDVIMN
                     LVGIFGWDIDFSLDIRRGDRFSIVYEELYRDDVKIRNGRILSAEFINDGRTFRAVHYT
                     DPQGDSDYYSPDGKSMRKAFLRSPVEFSRISSRFTNKRWHPALSKWRAHKGVDYAARR
                     GTPVRASGDGKVIHVGSKGGYGRLIVLRHGGRYTTAYGHLNGYARGIRHGAKVKQGQI
                     IGYVGSSGLATGPHLHYEFRVNGVHRNPLTVKLPEAQPVHSAYLDHFKENTRSYLSML
                     RLMNRTLADNTQ"
     gene            2576..3775
                     /gene="tyrS"
                     /locus_tag="VIW27_00840"
     CDS             2576..3775
                     /gene="tyrS"
                     /locus_tag="VIW27_00840"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY5738233.1"
                     /translation="MNFQKDAMRQIKRGCDEILLEPELEKKLTRGEPLRIKAGFDPTA
                     PDLHLGHTVLINKMRQFQELGHHIMFLIGDFTGMIGDPSGKSATRPPLSPEQIRQNAE
                     TSPQQVFKILDPDATEICFNSAWHGSKTAADMIRLASRHTVARMLERDDFARRYKNNQ
                     PISIHEFLYPLVQGWDSVEMRADVELGGTDQKFNLLVGRELMKAEGMEPQVILTMPIL
                     EGLDGVQKMSKSLDNYIAIEDPPGEMFGKLMSISDDLMWRYFELLSFRQLDEIERFRE
                     DVAAGANPRDIKFLLAEEIITRFHDAAAASAAREEFIARFAKGAMPDEMPAVSLSSES
                     GSIGIAALLKQAGLTSSTSESFRMIQQGAVRLDGEKVSSKDLSLERGTVVVAQVGKRK
                     FARVTVE"
BASE COUNT          763 a         1209 c         1312 g          773 t
ORIGIN      
        1 tcggccgcgt atacggcgcc aagcagggtt tcgcggctgg cattggtgat cgccggtatg
       61 ttgcccgcgc gccggttgat gcgttcacgc gccagccagg caaaagccgc cgcttcgacg
      121 tagtcggggt tcgtaccgag cgcatcggtg gtctgcaggc cgcagccggc cagcctgtcg
      181 cgcagccttt cgagcaggta gccgttgtgc gcgccgccgc cgcaaacgaa gcagttcgcc
      241 gctgcgccgg gcaggctcgc cagcgcagcg gcgatggtcg ctgcggtcag ttcggtcagc
      301 gtggcctgaa tatcctgtgc ctcgaagctc gccggcagac ggttttccag ccagtcggcg
      361 ctgaaatact cggtaccggt cgatttcggt gcggcagcgc ggaaataggg ctcgccggcg
      421 agaatcttct ccagcagcgc gtcgtcgacg cgaccgccgc gcgcccaggc gccgtcgtcg
      481 tcgtaggccg ctccccgatg cttttcgctc cagtaattca gcagcgtgtt gccggggccg
      541 gtgtcgaatc cgagcggcgt cgcgtcccgc ttcgccggca gccaggttat attggcaata
      601 ccgccgatgt tgacgaccgc acgatcctct tcggcagagt gaaacaggaa acggtggtag
      661 gcgggtgcca gcggcgcggc ctgaccgccg agcgcgatgt ctttgccacg aaaatcggcc
      721 accgtggtga tgccgcagcg agcggcgagc cggttggggt cgccgatctg cacgctatag
      781 ggcggatcgc tgtcggggct gtggcgcagg gtctggccgt ggcagccgag cgcggtcacc
      841 tgttgcgggt cgatgttcgc cgcctgcagg gcgcgctcca cggaatccgc atagacatca
      901 ccgagcctgg cgtcgagtgt gtacagcgca tcgagagaag tcatttgcgt gcggcataac
      961 tgctgcagcg cggcgcgcag ctccgcaggg taggcatcgg tggcgcagta gaggattcgc
     1021 ggcggaaatt gcccgaagtc gacgatcgcg acgtcgaccc cgtcgagact ggtgcccgac
     1081 atcaggccga cgtaaagctc gccgtcgcga ctcactgcgt gttatcggcc aacgtgcgat
     1141 tcatcagtcg cagcattgac agataagagc gggtgttttc cttgaagtga tcgaggtacg
     1201 ccgagtgtac cggctgggcc tcgggaagct tgacggtgag cggattacgg tgcacgccat
     1261 tgacgcgaaa ctcatagtgc aggtgcgggc cggtcgccag cccggagctg ccgacgtagc
     1321 cgatgatctg accctgcttg accttggcgc cgtgacgaat gccgcgcgca taaccgttca
     1381 ggtgcccgta ggcggtggtg tagcggccgc cgtggcgcag cacgatgagg cggccgtaac
     1441 cgcccttgct gccgacgtgt atgaccttgc cgtcgcccga agcccgtacc ggcgtacctc
     1501 gccgcgcggc atagtcgaca cccttgtgcg cgcgccactt ggacagtgcc gggtgccagc
     1561 gcttgttggt gaagcgcgag ctgatgcgcg aaaattcgac cgggctgcgc agaaaggcct
     1621 tgcgcatact cttgccgtcc ggcgagtagt aatcgctgtc accctgggga tcggtatagt
     1681 gaaccgcgcg aaaggtcctg ccgtcgttga tgaattcggc cgacaggatg cggccgttgc
     1741 gaatcttgac gtcgtcgcgg tagagttcct cgtagacgat gctgaagcga tcaccgcgcc
     1801 gaatatccag cgaaaagtcg atgtcccagc cgaaaatgcc caccaggttc atgatcacat
     1861 cctcgggaat atcggcggcg gcggcggctt cgaatagcga actttcgatt accccttcgc
     1921 ggaagaccgg ataggcttcg agctggtagt tgatcacctg gctgctgaag ccctggtcct
     1981 cggtgcgctg gatgaccagg gtgctggtca catcgggaga aaatttcatc tgccgcaaat
     2041 cgcgatcggc atcgagcagc aggcggattt cctgtccggg cttgatatag cgcaggcgac
     2101 gggtctgctc attgagccgc gccacccggt gggtagttgt cgacggaata cccttgccgg
     2161 caaagatcga ggccagcgtg tcgcccggct cgacaattac ggtttcccag ggcacctggt
     2221 cgatttcggc cgccgcccgt tggatgtctc gctgcagcga aagatcgggc tctggcaact
     2281 ccagcgaata gtgtaatcgc gccgacttgg gggtagaatc cgcggctccg gccgacggct
     2341 gccagtggga cacgctgaag ccgagcagca tgatgacggt tgccaccagc agcagtttta
     2401 gcagcacgcc gggatggaag tccgcgggcg atggcagcgg gttgaccgac cttgacaggc
     2461 aaacccgttc ggttttgaaa tcaagatctt gaaaactcat tagaaaattc gcctaagtta
     2521 ttggatcgat aaagataata ggcttgaaat tgtactcccc agacaggtat atagcatgaa
     2581 tttccagaaa gatgcaatgc ggcagataaa aagagggtgc gacgaaatcc tgctcgagcc
     2641 tgagctggag aaaaaactca cccgcggcga gcccttgcgc atcaaggcgg gtttcgaccc
     2701 gaccgcaccg gatctgcatc tcggtcatac cgtgctgatc aacaagatgc ggcagttcca
     2761 ggaactcggg caccatatta tgttcctgat cggcgatttt accggcatga tcggtgatcc
     2821 cagcggcaag tccgcgaccc gcccgccgct gtcgccggag cagattcggc aaaacgccga
     2881 aacctctccg cagcaggtgt tcaagatcct cgatcccgac gccaccgaga tttgcttcaa
     2941 ttccgcctgg cacggatcga aaaccgccgc cgacatgatt cggctcgcat ccaggcacac
     3001 ggtcgcgcgc atgctcgagc gcgacgattt cgccaggcgt tacaagaata accaaccgat
     3061 ttcgatacac gagttcctct atccgttggt gcagggctgg gattcggtcg agatgcgtgc
     3121 cgacgtcgaa ctcggcggca ccgaccagaa gttcaacctg ctggtgggcc gtgagttgat
     3181 gaaagccgag ggcatggagc cgcaggtgat cctgaccatg ccgatcctcg aggggctcga
     3241 cggtgtgcag aagatgtcga agtcgctcga taactacatc gccatcgagg atccgccggg
     3301 cgaaatgttc ggtaagctga tgtcgatcag cgacgacctg atgtggcgct atttcgagtt
     3361 gctgagcttt cgtcagctcg acgagatcga gcgttttcgc gaggacgtcg ccgccggcgc
     3421 caacccgcgt gacatcaagt tcctgctcgc cgaggagatc atcacccgct ttcacgacgc
     3481 cgcggccgcc agtgcggccc gcgaggaatt tatcgcgcgc ttcgcgaagg gcgcgatgcc
     3541 cgatgaaatg cccgcagtga gcctcagttc cgagtccggt agcatcggta tcgccgcgtt
     3601 actcaagcaa gccggcctga ccagtagcac ctcggaaagc tttcgcatga tccagcaggg
     3661 cgccgtccgc ctcgacggcg aaaaggtatc gagcaaggac ctgagcctcg agcgcggcac
     3721 ggtggtcgtg gcgcaggtcg gcaagcgcaa gttcgcccgt gtgacggtcg agtaaagcaa
     3781 ttcgcgggct ggcttcggca ccggcttcag cagcggcatc gaccatgcgc cgccaactcg
     3841 acggtagccg ccggctatcc ggtgttgcaa tccgctggtg cccgggcgcg gtggcgaacc
     3901 ggcggcaggg ggtgtagccg cctttttatg tgggctggta gcattctcca gtggccgtcg
     3961 cggtgcccgg attccgctat ctgcaggggt tgattgcgct atctgcgggg gtattttcga
     4021 ttattcccaa ataccgcttg acgcttcgtt tcgaacc
//
LOCUS       DATCZV010000036         8412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_2947, whole genome shotgun sequence.
ACCESSION   DATCZV010000036 DATCZV010000000
VERSION     DATCZV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /gene="ispH"
                     /locus_tag="VIW27_00845"
                     /gene_synonym="lytB"
     CDS             complement(<1..309)
                     /gene="ispH"
                     /locus_tag="VIW27_00845"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156582.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5738234.1"
                     /translation="MDILLANPRGFCAGVDRAIEIVERAIQLFGKPIYVRHEVVHNRH
                     VVDDLKQKGAIFVDELDQVPDDNYVIFSAHGVSRAVRQEAERRQLKVFDATCPLVTKV
                     H"
     gene            complement(309..761)
                     /locus_tag="VIW27_00850"
     CDS             complement(309..761)
                     /locus_tag="VIW27_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5738235.1"
                     /translation="MSQIQRNSLVTLHYRLGLPNGDVLEDNFDDEPMTVQLGQGEMAE
                     GLELALIGLRAGQEDTVDIGPELAFGYIDENLVRSIPRVEFDSDIELELGLIIEFSTD
                     TGDLLPGTILEFDEQRVRVDLNHPLAGQTVRYSVRIVSIDDDNEQLLH"
     gene            complement(758..1258)
                     /gene="lspA"
                     /locus_tag="VIW27_00855"
     CDS             complement(758..1258)
                     /gene="lspA"
                     /locus_tag="VIW27_00855"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5738236.1"
                     /translation="MRAEHPQLKWLWTSLAIILLDQATKQIAEHTLTPHQPVNVLPYF
                     DWYLTYNTGAAFSFLAGAGGWQRWLFTVIAVVISAVIVHWLIKLPRGETITAASLALI
                     LGGAIGNLIDRLYLGHVIDYIQIWLGSYPWPAFNIADAAISIGAVMLILSSLTSTGKS
                     GPNSSP"
     gene            complement(1266..4088)
                     /gene="ileS"
                     /locus_tag="VIW27_00860"
     CDS             complement(1266..4088)
                     /gene="ileS"
                     /locus_tag="VIW27_00860"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY5738237.1"
                     /translation="MADYKSTLNLPATDFPMRGNLAQREPERLQKWRDADLYQQIREA
                     RAGRKRWVLHDGPPYANGDIHIGHAVNKILKDMIVKSRTLNGEDAPYVPGWDCHGLPI
                     EHQVEKKLGKVGVKVEADEFRAACREFAESQIDKQREDFIRLGVLGDWYDPYLTMKFD
                     TEANIVRALGKIIENGHVTRGDKPVHWCTDCGSALAEAEVEYRDKTSPAVDVRFRAVD
                     DAAFTAAMQPADDGPGEGPVSVVIWTTTPWTLPANQAVAVSPKESYVLVQSGGERLLL
                     AEGLLEPALRRYGIDDYRVVARARGEALEGLRMSHPFYDREAVMVLGDYVTLEAGTGA
                     VHTAPGHGQDDFETGMKYGLEIYNPVGDNGCFLPSTELFAGEHVNKANGHIIEVLREH
                     DALLHDEKFNHSYPHCWRHKTPIIFRATPQWFISMDKEGLRSTAMAEIRKVRWVPSWG
                     QARIESMIEFRPDWCISRQRYWGVPIALFMHRETQQLHPRTAELIEEVALRIEKQGIQ
                     AWFSLTAEDLIGDDAADYTKVSDTLDVWFDSGTTHYSVLQRDPRCKFPADMYLEGSDQ
                     HRGWFHSSLLASCAMHGRAPYEQVLTHGFTVDAKGMKMSKSVGNVIAPIEVTRTLGAD
                     ILRLWVAATDYSGEMSISDEILKRTADSYRRIRNTIRFLLANTNDFDPAMNGVAADEL
                     LALDRWIVGETLKLQDELKQIYSDYLFHLAIQKIHNFCSETLGGYYLDVIKDRQYTTP
                     ADSRARRSCQTAQYLICEALVRWIAPILSFTADEIWEQMPGERGEFGVFTAEWFDGLF
                     PYAHPQVDISVWDNMEPVRDEVLRQLEILRQAGGIGSGLDAEVVIHADAATVERLRAI
                     GDELRFVLITSAATLRELDESHAGEAVKLADGGRFRVEAVASGHGKCVRCWHHTPEVG
                     SDKQHPELCARCAANVDGDGEARQFA"
     gene            complement(4081..5025)
                     /gene="ribF"
                     /locus_tag="VIW27_00865"
     CDS             complement(4081..5025)
                     /gene="ribF"
                     /locus_tag="VIW27_00865"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5738238.1"
                     /translation="MKLIRGLHNLGSPLAASAVTIGNFDGVHRGHQLVIRQLKRVAAD
                     AALPTVVIIFEPQPIEFFAPERAPGRLSRFREKIAYLKAQQIDYLLCLRFDRQLAAQT
                     AEDFVQQILLERLNTRHLVIGDDFHFGKNRQGNFRYLQEHSRRFGFAVDETETLVIDG
                     ERVSSTRIRRCIAAGDFGKAAELLGRPYSLSGRVAHGQKLGRQLGYPTINIKMGDKTL
                     IVRGIFAVHVKGIDNRPLEGVASIGTRPTVAGVDTILEVYILDFDRDVYGYSVEVEFL
                     HKLRDEEKFDSLEELSFNIGRDTQNAIEYFKTRRATVG"
     gene            complement(5206..6756)
                     /gene="murJ"
                     /locus_tag="VIW27_00870"
     CDS             complement(5206..6756)
                     /gene="murJ"
                     /locus_tag="VIW27_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020581551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY5738239.1"
                     /translation="MSALIKSTASVGSMTLLSRVLGLARDIVIARFFSASDAADAFFV
                     AFKIPNFLRRLFAEGAFSLAFVPVLTEYRSNFSAAETRDLIDRVAGTLGIILLLLSAL
                     GVAASPVLISVFAAGFLDQPDKFELTADMLRITFPYILLISMTAMSGGILNTWKRFTV
                     PAFTPVLLNLSLIGCAVYLSPRLELPITALAWGVLIAGIAQLLFQLPFLYREGLLPRP
                     KWGWPHPGVQKIVKLMIPALFGSSVAQINLLFDTFIASFLVSGSVSWLYYADRLLEFP
                     LGVFGIALATVVLPNLSEAHYRQGATHFNDTLRWAIQLSLLIAVPATFGLFLLAQPIL
                     ATLFQYGAFTAGDSLMSALSLMAYSLGLPAFILIKILASGFYARQDTRTPVRIGIQSM
                     FLNMALNVLFVVSMLNTGFVAPHVGLALATSLAAYFNAFRLARELRREQLLGELQTFS
                     IPLLRILAACLAMAALIYFMTPTIEGWSDWRWYRRLGELAWLILPAILCYGILLWIMG
                     FRRQHFVA"
     gene            7112..7372
                     /gene="rpsT"
                     /locus_tag="VIW27_00875"
     CDS             7112..7372
                     /gene="rpsT"
                     /locus_tag="VIW27_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5738240.1"
                     /translation="MANSASARKRAHQAEKRRQRNASRRSMMRTHIKATIAAIMSADR
                     SAAEAAFKKVVPVLDSAANHRLISHNKAARHKSRLNARIKEL"
     gene            7571..7846
                     /locus_tag="VIW27_00880"
     CDS             7571..7846
                     /locus_tag="VIW27_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738241.1"
                     /translation="MISSSVNRLRVVAIAGLLALGLASAPASASHGHAYVAPLAAFIA
                     FSAFAHHGHHSHHRHHYSQRPHYRPHHRHSHSHGGYRGGHKKHHRNW"
     gene            7853..>8412
                     /locus_tag="VIW27_00885"
     CDS             7853..>8412
                     /locus_tag="VIW27_00885"
                     /inference="COORDINATES: protein motif:HMM:NF038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor H family protein"
                     /protein_id="HEY5738242.1"
                     /translation="MKSLALVLVLLPAVLLETPARAEEALQSVRSAGKLVHDRSIDLG
                     AELEAREPLLRAAALEAEDYASSPRRQRDDGVANRHDQLFEIFSADARLLSDIDGDGF
                     HHAINVSFDVDVNYDDAMVYAKLYLSRDGEPWSQYFTTDLFYIHGDSVDDVYEVETEL
                     LSGYYPGYYAVLIEIYSLDHAYMVAS"
BASE COUNT         1597 a         2578 c         2690 g         1547 t
ORIGIN      
        1 gtgcaccttg gtgaccagcg ggcaggtggc gtcgaagacc ttcagctggc ggcgctcggc
       61 ttcctggcgt accgccctgg agacgccgtg ggcactgaag atcacgtagt tgtcgtcggg
      121 tacctggtcg agctcgtcga cgaatatcgc gcccttttgt ttcaggtcgt cgactacgtg
      181 gcggttgtgc acgacttcgt ggcgcacgta gatcggcttg ccgaacaact ggatcgcgcg
      241 ctcgacgatt tcgatcgcac gatcgacacc ggcacaaaac ccgcgggggt tggcgagcag
      301 tatgtccatc agtgcaggag ctgctcgttg tcgtcgtcga tcgacacgat gcgcacgctg
      361 tagcgcacgg tctgcccggc gagtggatga ttcagatcga cacgcacccg ctgctcgtcg
      421 aactcgagta tggtgcccgg cagcagatcg ccggtatcgg tcgagaattc gatgatcagg
      481 ccgagctcga gctcgatgtc ggaatcgaac tcgacgcgcg ggatcgagcg caccaggttt
      541 tcgtcgatat agccgaaagc gagctcggga ccgatgtcga cggtatcttc ctggcctgcg
      601 cgcaggccga tcagcgccag ctcgagaccc tcggccatct ccccctggcc gagctgcacg
      661 gtcatcggtt cgtcgtcgaa attgtcctcg agcacgtcgc cgttgggcag accgaggcga
      721 taatgcaggg ttacgaggct gtttctctgg atctggctca tgggctgcta ttcggtccgc
      781 ttttgccggt actggtcagg ctgctcaata ttaacatgac cgcgccgatg ctgatcgccg
      841 catcggcgat attgaacgcc ggccacgggt aactgccgag ccatatctgg atgtagtcga
      901 tgacgtggcc gaggtagaga cggtcgatca gattgccgat cgcgccgccc aggatcagcg
      961 ccagcgaggc cgcggtgatg gtctcgccgc gcggcagctt gatcagccag tgcacgatca
     1021 ccgcgctgat gacgacggcg attaccgtga acagccagcg ctgccagccg ccggcgccgg
     1081 ccaggaagct aaaagccgcg ccggtgttgt aggtcaggta ccagtcgaaa taaggcagta
     1141 cattgaccgg ctggtgcggg gtcagcgtgt gctccgcgat ctgcttggtg gcctggtcca
     1201 gcaggatgat ggcgaggctc gtccagagcc acttcagttg cggatgctcg gcgcgcatcg
     1261 tattctcagg cgaactgccg ggcctctccg tcaccgtcga cgtttgcggc gcagcgtgcg
     1321 cacagctccg ggtgctgctt gtcgctgccg acctcgggcg tgtggtgcca gcagcgcacg
     1381 cacttgccat gcccgctggc gaccgcctcg acacgaaacc ggccgccgtc ggcgagcttt
     1441 accgcttcgc cggcatgtga ctcgtcgagt tcccgcagcg tcgccgccga ggtaatcagg
     1501 acgaagcgca gttcgtcgcc gatggcccgc aggcgctcga cggtcgcggc gtcggcgtgg
     1561 atcacgacct cggcatcgag accggaaccg atgccgcctg cctggcgcag gatctccagc
     1621 tggcgcagca cctcgtcgcg caccggctcc atgttgtccc ataccgagat atcgacctgc
     1681 ggatgcgcgt aggggaacag cccgtcaaac cactcggcgg taaatacgcc gaactcgccg
     1741 cgttctcccg gcatctgttc ccagatctcg tccgcggtga aggacaggat cggcgcaatc
     1801 cagcgcacca gtgcctcgca gatcaggtac tgcgcggtct ggcaggagcg gcgcgcgcgc
     1861 gaatcggccg gcgtcgtgta ctggcggtcc ttgatcacgt cgaggtagta accgccgagc
     1921 gtctcggagc aaaaattgtg aattttctga atcgcgaggt gaaacaggta atcgctatag
     1981 atctgcttca gctcgtcctg caatttcagc gtctcaccga cgatccagcg gtcgagcgcg
     2041 agcaattcgt cggcggcgac gccgttcatg gcgggatcga aatcgttagt gttggcgagc
     2101 aggaaacgaa tcgtgttgcg aatacgccgg taggaatcgg cggtgcgctt gaggatctcg
     2161 tcggaaatcg acatttcgcc cgaataatcg gtggccgcga cccacaggcg cagaatgtcg
     2221 gcgccaagcg tgcgcgtcac ttcgatcggc gcgatcacgt tgccgaccga tttcgacatc
     2281 ttcatgcctt tggcgtcgac cgtgaaaccg tgggtcagta cctgctcgta gggcgcccgg
     2341 ccgtgcatcg cgcaggacgc cagcagcgac gaatggaacc agccgcggtg ctggtccgag
     2401 ccctcgaggt acatgtcggc cgggaacttg cagcgcgggt cgcgctgcag cacgctgtaa
     2461 tgcgtggtgc cggaatcgaa ccagacatcg agcgtgtcgg acacctttgt atagtcggcc
     2521 gcatcgtcgc cgatcaggtc ctcggcggtg agcgaaaacc aggcctggat gccctgcttt
     2581 tcgatgcgca gcgcgacttc ctcgatcagt tccgcggtac gcgggtgcag ctgctgggtt
     2641 tcgcggtgca tgaacaacgc gatcggcacg ccccagtagc gttgccggga aatgcaccag
     2701 tcggggcgga actcgatcat gctttcgatg cgcgcctggc cccaggaagg tacccagcga
     2761 accttcctga tctcggccat cgcggtcgag cgcaggcctt ccttgtccat gctgatgaac
     2821 cattgcggcg tggcgcggaa aatgatcggc gtcttgtgcc gccagcagtg cgggtagctg
     2881 tgattgaatt tctcgtcgtg cagcagcgcg tcgtgctcgc gcagcacctc gatgatatgg
     2941 ccgttagcct tgttgacgtg ctcgccggca aatagctcgg tgctcggcag gaagcagccg
     3001 ttgtcaccga ccgggttgta aatttccagc ccgtatttca tgccggtctc gaagtcgtcc
     3061 tggccgtgcc cgggcgcggt atgcacggcg ccggtaccgg cctcgagagt tacgtaatcg
     3121 ccgagtacca tgaccgcctc gcgatcgtag aacgggtgcg acatgcgcag gccctcgagt
     3181 gcctcgccgc gcgcccgcgc gaccacgcga tagtcgtcga tgccgtaacg ccgcagtgcc
     3241 ggctccagca agccctcggc gagtagcaac cgttcaccgc cgctttgtac cagcacatag
     3301 ctttccttcg ggctgacggc gaccgcctgg ttggcgggca gcgtccatgg ggtcgtggtc
     3361 cagatcacca ccgacaccgg accttcgccg ggaccgtcgt ccgccggctg catcgccgcg
     3421 gtaaaggccg cgtcgtcgac cgcgcgaaaa cgcacatcga ccgccggcga ggtcttgtcg
     3481 cggtactcga cctcggcctc ggccagcgcc gaaccgcaat cggtacacca gtgcaccggc
     3541 ttgtcgccgc gcgtgacgtg gccgttttcg atgatcttgc cgagcgcacg cacgatattg
     3601 gcctcggtat cgaatttcat cgtcaggtaa gggtcgtacc agtcgccgag cacgccgaga
     3661 cggatgaaat cctcgcgttg cttgtcgatc tggctctccg cgaattcgcg gcaggcggcg
     3721 cggaattcgt ccgcctcgac cttgacgccg accttgccga gtttcttttc cacctggtgc
     3781 tcgatcggca ggccgtggca atcccagccg ggcacgtagg gcgcatcctc gccgttgagc
     3841 gtgcgcgact tgacgatcat gtccttcaga atcttgttga ccgcgtggcc gatatgaatg
     3901 tcgccgttgg catagggcgg cccgtcgtgc agcacccagc gcttgcgtcc cgcgcgcgcc
     3961 tcgcgtattt gttggtagag gtcggcatcg cgccattttt gcaggcgctc gggctcgcgc
     4021 tgggcgaggt tgccacgcat cgggaaatcc gtagcgggca ggttcagtgt cgatttgtaa
     4081 tcagccaacg gttgctcgtc tcgtcttgaa gtattcgatt gcgttctgcg tatcgcggcc
     4141 gatattgaaa ctcaattcct cgagcgaatc gaatttttcc tcgtcgcgca gcttgtgtaa
     4201 aaactcgacc tcgacgctat acccgtatac gtcgcggtcg aaatccagaa tgtaaacctc
     4261 gagaatcgtg tcgacgccag ccaccgtcgg acgcgtgccg atgctggcga cgccctcgag
     4321 cggtcgatta tctatgcctt tgacgtgaac cgcaaatatt ccgcggacga tcagggtttt
     4381 atcgcccatc ttgatgttga tcgtcgggta gccgagctgg cgccccagtt tctgtccgtg
     4441 cgcgacgcgg cccgacagcg aatagggccg tccgagcaat tcggcggcct tgccgaaatc
     4501 gccggccgcg atgcagcggc gaatacgcgt gctgctgacg cgctcgccgt cgattaccag
     4561 ggtttcggtt tcgtcgaccg cgaaaccgaa gcgccggctg tgctcctgca ggtaacggaa
     4621 gttgccctgg cggtttttgc cgaagtggaa atcgtcgccg atcaccaggt ggcgggtgtt
     4681 gaggcgctcg agcagtatct gttgcacgaa atcctcggcc gtctgcgccg ccagctgccg
     4741 gtcgaagcgc aggcacagca ggtagtcgat ctgctgtgcc ttcaggtagg cgatcttctc
     4801 gcgaaaccgc gacaggcggc cgggagcccg ctcgggggcg aagaactcga tcggctgcgg
     4861 ttcgaagatg attaccacgg tgggcagcgc ggcatccgcc gccacccgct tcagctggcg
     4921 aatcaccagc tggtgaccac ggtgcacgcc gtcgaaatta ccgatggtca cagcgctcgc
     4981 cgccagcggg ctgcccaggt tgtggaggcc acgaatgagc ttcatctcag cctgaacatt
     5041 gtctcagtat gccgagcgat aacgggggca cagctggatc gggcagcgcc ggaacagggc
     5101 ccgggccgat ttcactacga gctctcaagg tatttgatcc actcacacta atattttgga
     5161 attgtaccgg tttaagatga ggttcgagac cgattggtgc aatattcagg cgacgaagtg
     5221 ttggcgacga aatcccatta tccaaagcag gattccgtag cacaagatgg ccggcagaat
     5281 aagccaggcg agttcgccca ggcggcggta ccagcgccag tcgctccagc cctcgatggt
     5341 cggggtcatg aaatagatca gtgccgccat cgccaggcag gcggcgagaa tgcgcagcaa
     5401 cgggatgctg aaggtctgca actcgccgag caattgttcg cggcgcagtt cgcgcgccaa
     5461 ccgaaaggcg ttgaaatagg ccgccagcga ggtcgccagc gccaggccga catgcggcgc
     5521 gacgaaaccg gtgttcagca tcgagaccac gaacagcacg tttagcgcca tgttgagaaa
     5581 cattgactgg atgccgatgc gtaccggcgt gcgcgtgtcc tggcgcgcgt agaagccgct
     5641 ggcgagtatc ttgatcagga tgaaggccgg caggccgagg ctgtaggcca tcagcgacag
     5701 cgctgacatc agcgaatcgc cggcggtaaa ggcgccgtac tggaacagcg tggcgagaat
     5761 cggctgcgcc agcaggaaca gcccgaaagt cgccggcacc gcgatcaaca gcgacagctg
     5821 gatcgcccag cgcagcgtat cgttgaaatg tgtcgcaccc tgccggtagt gcgcttccga
     5881 caggttcggc agcaccacgg tagccagcgc gatgccgaac acgccgagcg gaaactcgag
     5941 caggcggtcg gcatagtaca gccaggagac gctgccgctg accaggaacg aggcgatgaa
     6001 ggtatcgaac agcaggttga tctgggccac cgaggagccg aacagcgccg gaatcatcag
     6061 tttgacgatt ttttgcacgc cgggatgcgg ccagccccat ttcggccgcg gcagcaggcc
     6121 ttcgcggtag aggaacggca gctggaacag cagctgggcg atgccggcaa tcagcacgcc
     6181 ccaggccagc gcggtaatcg gtagttcgag gcgcgggctc aggtagaccg cgcagccgat
     6241 caggctcagg ttcagcagca ccggcgtgaa tgccggcacc gtgaagcgct tccaggtatt
     6301 gaggatgccg cccgacatcg cggtcatcga gatcagcaat atataaggaa aggtgatgcg
     6361 cagcatgtcg gcggtcagct cgaacttgtc cggctggtcg agaaagccgg ccgcgaacac
     6421 gctgatcagc accggcgagg cggcgacgcc gagcgccgac agcaacagca ggatgatgcc
     6481 gagggtgccg gcgacgcggt cgatcaggtc gcgggtctcg gcggccgaaa aattgctgcg
     6541 gtactcggtc agcaccggca cgaaggccag cgagaacgcg ccctcggcaa acagccggcg
     6601 caggaaattg gggatcttga aagccacgaa gaaggcgtcg gcggcatcgc tggcggaaaa
     6661 gaagcgggca atgacgatat cgcgcgccag gcccagcacg cgcgacagca gtgtcatcga
     6721 gcctaccgag gcagtagatt tgatcagcgc actcattgag cggaattcga gaatttttcc
     6781 ggggctttga agttggcgga ttctagcccg gatatttcgt taagggaacg aaactcgggg
     6841 gaattctggc aaccggggca gccgggcctg gccccgccac tgcgccgcgg ggccgccgcc
     6901 gcgggccctg gggccaagta ccgggggggg gtggcgtcgc ggcgcccgcg aggctgtcga
     6961 tgccgcgcaa acggtcaatg cgtgtccccc gcatgcggca ttcaggcctg tatccagggc
     7021 ctggaatcgc gaggatttca ggttgacagc gccgggcgca gtgaagataa tgcgcgcctc
     7081 gttttttcaa gctcgagcac aggagcaaac cttggcaaac tcagcatccg cacgcaagcg
     7141 tgcccatcag gccgaaaaac gccggcaacg taatgccagc cgtcgctcga tgatgcggac
     7201 tcatatcaag gcaaccatcg ccgcgatcat gtcggccgac cgcagcgcgg ccgaggccgc
     7261 cttcaagaaa gtcgtgccgg tgctcgacag tgccgccaac catcgactga tcagccacaa
     7321 caaggcagcg cgtcataaga gccgtctgaa cgcgcgcatc aaggagctct gagcagttcc
     7381 aagagatagc gatcgaggcc ggggttttcc cggccttttt tttgcccgct gacggtgttc
     7441 gaagccgccc cgtcgcggct ccttccaggc ggctcgcaat cagttcacaa aaccgggaat
     7501 cacgattcag cctggattca gtctctaggc gctaaattgg cgtcaccttc aagcaacgat
     7561 ggagagacag atgatcagct cgagtgtaaa caggctacgc gtcgtggcga ttgccggatt
     7621 gcttgccctg gggctggcca gtgcgcccgc aagtgccagt cacggtcacg cctatgtcgc
     7681 gccgctggcg gcattcatcg cgttcagcgc gttcgcgcat cacggtcacc atagccacca
     7741 ccggcaccac tattcgcagc gcccgcacta tcggccgcat caccgccaca gtcacagcca
     7801 tggcggctat cgcggcgggc acaagaagca tcaccgcaac tggtagcgga cgatgaagtc
     7861 actggcttta gtgctggtac tgctgccggc cgtgctgctc gagacgccgg cgcgggccga
     7921 ggaagcgctg caatcggtgc gctcggccgg caaactggtg cacgacaggt ccatcgatct
     7981 cggtgccgag ctcgaagcgc gcgaaccgct gctcagggcc gccgcgctcg aagccgagga
     8041 ctatgcctct tcgccgcggc gccagcgaga cgacggggtc gccaaccgcc acgaccagtt
     8101 gttcgaaatt ttcagcgccg acgccaggct gttgtccgat atcgacggag acggctttca
     8161 tcatgccatc aatgtttcgt tcgacgtcga tgtcaattac gacgacgcca tggtctacgc
     8221 caagctctac ctgagtcgcg acggcgagcc ctggagccag tacttcacca ccgatctgtt
     8281 ctacatacat ggcgacagcg tcgacgatgt ctacgaggtc gagaccgagc tgctgtcggg
     8341 ctattacccg ggctattacg cggtgttgat cgaaatctat agcctcgacc atgcctacat
     8401 ggtcgccagc ga
//
LOCUS       DATCZV010000037         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_56023, whole genome shotgun sequence.
ACCESSION   DATCZV010000037 DATCZV010000000
VERSION     DATCZV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56023"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2389)
                     /gene="ppsA"
                     /locus_tag="VIW27_00890"
     CDS             complement(17..2389)
                     /gene="ppsA"
                     /locus_tag="VIW27_00890"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY5738243.1"
                     /translation="MTENIVPLERLGMNDVNRVGGKNASLGEMIGKLGDLGVEVPGGF
                     ATTADAFNDFLDAAGIRERIHQRLEQLDTDDVRALAAAGEEIRGWIIDAPLPVGLRDD
                     IAAAYAAMAVDGSDATVAVRSSATAEDLPDASFAGQQETFLNVRGIDRVLHAIRLVFA
                     SLFNDRAISYRVHQGFAHADVSLSAGVQRMVRSDLAASGVMFSIDTESGFEDAVFITS
                     SYGLGETVVQGAVNPDEFYVYKKNLDTATRSILLKNRGSKLIKMIYADDSDPENAVRT
                     VDVDADDRLRFSIDDDEITALARIAVTIEQHYGRPMDIEWAKDGENGKLYIVQARPET
                     VQSRAGQIIERYRLKEKSRVLVDGRAVGNRIGRGPAKVISDLSQMDQIEAGDVLVTDM
                     TDPDWEPIMKRAAAIVTNRGGRTCHAAIIARELGIPALVGTGSCTTDIAHGSEVTVSC
                     AEGDTGYVYEGLLDFEHQRHELSQMPEIPLKISMNVGNPERAFSFSRLPHAGIGLARL
                     EFIISNMIGIHPRALLEFDAMDAGLKSSIEQRIGGYAGPVEYYVDRLSEGIATLAAAF
                     APERVIVRMSDFKSNEYAHLLGGDVFEPHEENPMLGYRGASRYISDEFKPSFRLECEA
                     IKRVREDMGLRNIEVMIPFVRTLGEARAVHALLEANGLVRGVDDFRVIMMCEIPSNVI
                     LAAEFLEYFDGFSIGSNDLTQLTLGLDRDSGLVASAFDERDPAVMKLLAQAISACRDS
                     GKYVGICGQGPSDHPELAEWLLEQGISSISLNPDTVIDTWLFMAEQNKSS"
     gene            2481..>3286
                     /locus_tag="VIW27_00895"
     CDS             2481..>3286
                     /locus_tag="VIW27_00895"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY5738244.1"
                     /translation="MLDSKRLVYFLSNGTAITAETLGLSLLAQFPDRHFQTRTVPFVD
                     TLEKAHAVSDEIDRAGDDAGHLPIVICTMGDEALMKIVIQTRALVIDPFGDILPRLEK
                     VLAAEHHQPGQSHRTVNPSLYESRIDAIDYAMIHDDGGKTQNYDQAEIILIGVSRSGK
                     TPSCLYLALQFGIRAANYPLTEEDMESEKLPVPLAPFRDRLYGLTTDLNRLVNIRQAR
                     RPNSPYSSLKQCRYELRAAEEMYRRHDIRYINTAEMSVEEIAARIIRDNDL"
BASE COUNT          615 a         1030 c         1011 g          630 t
ORIGIN      
        1 cgtattctgc catgggttag ctggatttgt tttgctcggc catgaacagc caagtgtcga
       61 ttacggtatc cgggttgagg gatatgctgc taataccctg ttccagcagc cattcggcga
      121 gctcggggtg atcggaaggg ccctgaccgc agataccgac atacttgccg ctgtcgcggc
      181 aggcgctgat cgcctgtgcg agcagtttca tcaccgcggg atcgcgttcg tcgaaagccg
      241 atgccaccag ccccgaatcg cggtcgagac cgagcgtcag ctgcgtcagg tcattggaac
      301 cgatcgagaa accgtcgaaa tactcgagga actcggcggc gagaatcacg ttggacggaa
      361 tctcgcacat catgataacg cggaaatcgt cgaccccgcg caccagcccg tttgcctcca
      421 gcagggcatg caccgcgcgc gcctcgccga gggtgcgtac gaacggaatc atgacttcga
      481 tgttcctcag acccatgtcc tcgcgtaccc gcttgatcgc ctcgcattcg agccgaaagc
      541 tcggcttgaa ctcgtcgctg atgtagcgcg atgcgccgcg gtagccgagc atcggatttt
      601 cctcgtgcgg ctcgaagaca tcgccgccga gcaggtgggc atactcgttc gatttgaagt
      661 ccgacatgcg cacgatcacg cgctccgggg caaaagccgc ggccagtgtc gcgattcctt
      721 cgctgagccg gtcgacgtag tattcgaccg gcccggcgta accgccgata cgctgctcga
      781 tcgatgattt caggccagcg tccatggcat cgaattcgag cagcgcgcgc ggatggatgc
      841 cgatcatgtt gctgatgatg aactccagcc gcgcgagacc gatgccggcg tgcggcagcc
      901 gcgaaaagct gaaggcgcgc tccgggttgc cgacgttcat cgagatcttg agggggatct
      961 cgggcatttg cgacagttcg tggcgctggt gctcgaagtc gagcaggccc tcgtagacat
     1021 agccggtatc gccctcggcg caggacacgg tgacctcgct gccatgcgcg atatcagtgg
     1081 tacagctgcc ggtaccaacc agcgccggaa tgccgagttc gcgggcaata atcgccgcgt
     1141 ggcaggtgcg gccgccgcgg ttggtgacga tcgccgcggc gcgcttcatg atcggttccc
     1201 agtcggggtc ggtcatgtcg gtgaccagta cgtcgccggc ctcgatctgg tccatttgcg
     1261 agaggtcgga aatcaccttc gccgggccgc ggccgatgcg gttgccgacc gcgcgcccgt
     1321 cgaccagcac gcgtgatttt tctttgagcc gataacgttc gatgatctgg ccggcacgcg
     1381 attgcacggt ttccgggcgc gcctgcacga tgtagagctt gccgttctca ccgtccttgg
     1441 cccactcgat atccatcggc cgcccgtagt gctgctcgat ggtgaccgcg atgcgcgcca
     1501 gcgcggtgat ctcgtcgtcg tcgatcgaga aacgcagccg gtcatcggca tcgacgtcga
     1561 ccgtgcgcac cgcgttttcg ggatcgctgt catcggcgta aatcatcttg atcagcttgc
     1621 tgccgcgatt tttcagcagg atgctgcgtg tcgctgtgtc gagattcttc ttgtagacgt
     1681 agaactcgtc cgggttgacc gcaccctgca ccacggtctc gccgaggccg tagctgctgg
     1741 tgatgaacac cgcgtcctcg aaacccgact cggtatcgat gctgaacatc acgccgctgg
     1801 ccgccaggtc gctgcgcacc atgcgctgta cgccggccga cagcgacacg tcagcgtggg
     1861 cgaaaccctg gtgcacgcga taggagatcg cgcggtcgtt gaacagcgag gcgaatacca
     1921 ggcgaatcgc gtgcagcacc cggtcgatgc cgcgcacgtt gagaaaggtt tcctgctgcc
     1981 cggcgaacga tgcgtccggc aaatcctccg cggtcgccga agagcgcacc gctaccgtgg
     2041 cgtcactgcc gtccaccgcc atcgcggcgt aggccgcggc aatatcgtcg cgcagcccga
     2101 caggcagcgg ggcgtcgatg atccagccgc gtatttcttc gccggccgcg gccagggcgc
     2161 gtacgtcgtc agtgtcgagt tgctccaggc gctggtgtat gcgttcgcga atcccggctg
     2221 cgtcgaggaa gtcgttgaat gcgtctgcgg tggttgcgaa tccgcccggc acctcgacgc
     2281 cgagatcgcc gagcttgccg atcatctcgc cgagcgatgc gtttttaccg ccgacacgat
     2341 tgacgtcgtt catgccgagc cgctcgagcg gcacgatatt ttcagtcata ggccaggatt
     2401 tccgtcgtgt tagtcgatga cgcgcaagta tactagtata tcgattcgaa accgagaccg
     2461 atcaggcaaa gccgagcatc atgctggaca gcaagcgact ggtatatttt ctctccaatg
     2521 gcaccgcgat aaccgccgaa accctcggcc tgagcctgtt ggcgcagttt cccgaccgcc
     2581 actttcagac ccgcacggtg ccgttcgtcg acacgctcga aaaggcccat gcggtcagcg
     2641 acgaaatcga ccgcgccggc gacgacgcgg ggcacctgcc gatcgtgatc tgcactatgg
     2701 gcgacgaagc attgatgaag atcgtcatcc agacccgtgc gctggtgatc gatccgttcg
     2761 gtgacatttt gccgcgactc gaaaaggtac tggccgccga acaccaccag ccggggcaat
     2821 cgcaccgcac cgtcaatccg tcgctgtacg aatcgcgcat cgacgcgatc gattacgcga
     2881 tgatccacga cgacggtggc aagacccaga actacgatca ggcggaaatc atcctcatcg
     2941 gcgtgtcgcg ttccggcaag acgccgagct gtctttatct ggcgctgcag ttcggcatac
     3001 gcgcggccaa ttacccgctt accgaggaag acatggaatc ggaaaagctg ccggtgccgc
     3061 tggcgccgtt tcgcgaccgg ctatatgggt tgaccaccga cctgaaccgg ctggtcaata
     3121 ttcgccaggc aaggcggccg aacagcccct attcgtcgct caagcagtgc cgctacgagc
     3181 tgcgcgccgc cgaggagatg taccgtcgcc acgacatcag gtacatcaat accgcggaga
     3241 tgtcggtgga ggaaatcgcc gcgcgcatca ttcgcgacaa cgacct
//
LOCUS       DATCZV010000038         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_161775, whole genome shotgun sequence.
ACCESSION   DATCZV010000038 DATCZV010000000
VERSION     DATCZV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161775"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="mutL"
                     /locus_tag="VIW27_00900"
     CDS             <1..903
                     /gene="mutL"
                     /locus_tag="VIW27_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637474.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEY5738245.1"
                     /translation="LDARELDVNVHPQKHEVRFRNSRAVHDFLFRSLHQALAEVQPER
                     QVESPAFSLHREMPAAAPSQAGLGLGFGGGDRSARVREQLQSYAALLDPGPANDAQSE
                     APAELPPLGFAVAQLKGIYILAENRDGLVVVDMHAAHERIVYERMKRHAADEKLISQP
                     LLVPLNFNLSRAEGDLVEEQGEALRRLGFEVERLGPEQVRLRALPALLKHADGEQLLR
                     DVLADFAEHGRSERIDEVRDEMLSTMACHASVRANRQLTLAEMNALLRDIEHTERSGQ
                     CNHGRPTWKQLSLGQLDKLFLRGQ"
     gene            932..1849
                     /gene="miaA"
                     /locus_tag="VIW27_00905"
     CDS             932..1849
                     /gene="miaA"
                     /locus_tag="VIW27_00905"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY5738246.1"
                     /translation="MGATATGKTELALDIAARFPVEIVSVDSALVYRGLDIGTAKPSA
                     EILSSVPHHLVDIVEPTESYSAWNFVEQCREKVQEIAARGRIALLAGGTMLYYHALEQ
                     GLNRLPEADLELRRRLDSEARRHGWPAMHARLAAVDPDSAARIKPGDSQRVQRALEVY
                     EIAHEPLSVLQRRRATGYGGKIEKIILVPGDRKVLHQHIERRFLAMLEQGFIDEVEGL
                     KRRGDLDLSLPSMRCVGYRQIWQYLDGESSRVQMIDRAVAATRQLAKRQLTWLRKQPQ
                     EKAFDCLNYRKDAIFRGVEAVFRDFDWNR"
     gene            1935..2183
                     /gene="hfq"
                     /locus_tag="VIW27_00910"
     CDS             1935..2183
                     /gene="hfq"
                     /locus_tag="VIW27_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY5738247.1"
                     /translation="MSKGQMLQEPYLNALRREHVPVSIFLVNGIKLQGQIESFDQFVV
                     LLKNNVSQMVYKHAISTIVPARNVHIDHPHADEDEGNR"
     gene            2222..>2868
                     /gene="hflX"
                     /locus_tag="VIW27_00915"
     CDS             2222..>2868
                     /gene="hflX"
                     /locus_tag="VIW27_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5738248.1"
                     /translation="MFEFERPRGGERALLVHINFPQSSDQDDFDEFVELVRSAGVDDV
                     EVITGSRHQASVGSFIGKGKLEEVRSRVEALGVEVVLFNHSLSPSQERNLERELKCRV
                     LDRTGLILDIFAQRAQSFEGKLQVELAQLKHLSTRLVRGWTHLERQKGGIGLRGPGET
                     QLETDRRLLGKRIKYLNNRLVKVSRQREQSRQARTKAEIPTVSVVGYTNAGKSTL"
BASE COUNT          582 a          880 c          904 g          502 t
ORIGIN      
        1 ctcgatgcgc gcgaactcga tgtcaacgta cacccgcaga aacacgaggt ccgctttcgc
       61 aactcccgcg cggtgcacga tttcctgttc cgcagcctgc accaggcgct cgccgaggtg
      121 cagcccgaac ggcaggtcga atcgccggcg ttttcgctgc atcgcgagat gcccgccgcg
      181 gcgccgtcgc aggcggggct cgggctcggc ttcggcggcg gcgatcgctc ggcacgggtc
      241 agggagcagc tgcaatccta cgcggcgctg ctcgacccgg gcccggcgaa tgacgcccaa
      301 tccgaggcgc cggccgaact gccgccgctg ggattcgcgg tcgcgcagtt gaagggcatc
      361 tacatcctcg ccgaaaaccg cgacggactg gtggtggtcg acatgcacgc cgcgcacgag
      421 cgcatcgtct acgagcgtat gaagcggcat gccgccgacg aaaagctcat ttcccaaccg
      481 ctgctggtgc cgttgaattt caatctcagc cgtgccgagg gagacctggt cgaggagcag
      541 ggcgaagcct tgcgccggct gggcttcgag gtcgagcggc tgggccccga gcaggtcagg
      601 ctgcgcgcgc tgccggcgct gctcaagcac gccgacggcg agcaattgct gcgcgacgtg
      661 ctcgccgatt tcgccgagca cgggcgatcc gagcgcatcg acgaggtgcg tgacgaaatg
      721 ctgtcgacca tggcctgcca tgcctcggtg cgcgccaatc ggcagttgac gctcgccgaa
      781 atgaatgcgc tgctgcgcga tatcgagcac accgagcgca gcggccaatg caatcacggg
      841 cgtccgacct ggaaacagct cagcctgggg caactcgaca agctgtttct gcgcggccag
      901 taaggtgacc gaacccgcgg tcatctgcct gatgggggct accgccaccg gcaagaccga
      961 gctggcactg gatatcgccg cgcgcttccc ggtggagatc gtcagcgtcg attcggcgct
     1021 ggtctaccgc ggcctcgata tcggcactgc caagccgtcg gcggagatcc tttccagcgt
     1081 accccaccac ctggtcgata tcgtcgaacc gacggagtct tactcggcct ggaacttcgt
     1141 cgagcaatgc cgcgaaaagg tgcaggagat cgccgcacgc gggcgcatcg cgctgctcgc
     1201 gggcggcacc atgctctatt accacgcgct cgaacaaggg ctcaaccggc tgcccgaagc
     1261 cgacctggaa ttacgccggc gcctcgatag cgaagcccgc cgccacggct ggccggcgat
     1321 gcatgcgcgt ctcgcggcag tcgatcccga cagcgcggca cgcatcaagc ccggcgattc
     1381 gcagcgcgtg cagcgcgcgc tcgaggtata cgaaatcgcc cacgagccgt tatcggtgtt
     1441 gcagcgccgg cgggcgacgg gttacggcgg caaaatcgaa aaaatcatcc ttgtacccgg
     1501 tgaccgcaaa gtcttgcatc agcacatcga acggcgcttc ctggcgatgc tcgagcaggg
     1561 tttcatcgac gaggtcgagg gcttgaaaag gcgtggtgac ctcgatctgt cgctaccgtc
     1621 gatgcgctgc gtcggttatc ggcagatctg gcaatacctg gacggcgaat cgagccgggt
     1681 gcaaatgatc gacagggcgg ttgccgcgac ccgccagctg gccaagcggc agctcacctg
     1741 gttgcgcaaa caaccgcagg aaaaggcctt cgattgcttg aattaccgta aagatgctat
     1801 atttcgagga gttgaagccg tttttcggga ctttgactgg aaccgttaaa aagcagggaa
     1861 tcatacattc cgctgatcaa tggaagcatg gtcatcagcg gatctaaaat aaaaaaatca
     1921 atggagaatt aattatgtcc aaagggcaaa tgttacaaga accctacctc aatgcactgc
     1981 gaagagagca tgttccggta tcgattttcc tggtcaacgg catcaagctg cagggacaga
     2041 tcgagtcgtt tgatcaattc gtcgttttgc tgaagaataa tgtcagccag atggtgtaca
     2101 agcacgcgat ttcgaccatc gtaccggcgc gtaacgtcca tatcgaccat cctcatgcgg
     2161 atgaagacga gggcaaccgt taacacgtcg ataagtgaca tgaccggcac cgggggtctc
     2221 gttgttcgaa tttgaacgcc cgcgaggcgg tgaacgcgcc ctcctggtcc atatcaattt
     2281 tccgcagtcg tccgaccagg atgatttcga tgaattcgtc gaactggtgc gttcggcggg
     2341 cgtcgacgac gtagaagtga tcaccggcag ccggcaccag gcctcggtcg gcagttttat
     2401 cggcaagggt aaactcgagg aggtcagatc gcgggtcgag gcgctcggcg tcgaggtggt
     2461 gctgttcaac cattcgctgt cgccatcgca ggaacgcaac ctcgagcgcg agctgaagtg
     2521 ccgcgtgctc gatcgcaccg gcctgatact cgatatcttc gcgcagcgcg cgcagtcttt
     2581 cgagggcaag ctgcaggtcg agctggcgca attgaagcac ctgtcgaccc ggttggtgcg
     2641 cggctggacc cacctcgagc ggcaaaaggg cggtatcggc ctgcgcggtc ccggcgaaac
     2701 ccagctggaa accgaccgcc gcctgctcgg caagcgcatc aagtacctca acaaccgcct
     2761 cgtcaaggtg tcgcggcagc gcgagcaaag ccgccaggcg cgcaccaagg cggagatacc
     2821 gacggtctcg gtggtgggtt acaccaacgc cggcaagtcg acgctgtt
//
LOCUS       DATCZV010000039         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_56188, whole genome shotgun sequence.
ACCESSION   DATCZV010000039 DATCZV010000000
VERSION     DATCZV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56188"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VIW27_00920"
     CDS             complement(<1..999)
                     /locus_tag="VIW27_00920"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY5738249.1"
                     /translation="MANTVVIIGTQWGDEGKGKIVDLLTDRANAVVRFQGGHNAGHTL
                     VIDGNKTVLHLIPSGVLRDGVNCLIGNGVVLSLEALIREMTQLEDSGVPARERIVVSD
                     ACPLILPSHIALDQAREKARGSNAIGTTGRGIGPCYEDKVARRGLRLGDLANEAHFSA
                     RLGELLDYHNFVLEHYFGDSPLDFQQMLDECLQQGEQVSGMVGDVATILHDLIERGEP
                     VMFEGAQGALLDIDHGTYPFVTSSTTTAGGAAAGSGVGPRDIDYILGIVKAYTTRVGA
                     GPFPTELDDACGQHMGDRGHEFGATTGRKRRCGWLDLVALKRSLKINSVSGLCITKL"
     gene            complement(1009..2208)
                     /locus_tag="VIW27_00925"
     CDS             complement(1009..2208)
                     /locus_tag="VIW27_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY5738250.1"
                     /translation="MNEKSTKRWLLPDGVQETLPPDALAVELLRHEILQIFHSWGYDL
                     VMPAMIEYMDSLLTGTAHALDTRTFALVDQLSGKQMGVRSDMTPQVARIDAHQLVDDE
                     RHHRVSRLCYCGHLLHAIGDGISASRTPLQIGAEIFGSESIGADLEIVSLMVEALHAV
                     GLGEMSLDIGHVGIFRGLVAHTSLDDEQENRLFDMLQRKSIPDLQVYLQQLPLDDRQR
                     EQISRLALLSGDESVIDEARGIYSDAGDAVLATLDTMSRVVQSLRRMYPDTSIHCDLS
                     ELRGYSYHTGLVFAAFLPGQGREIARGGRYDDVGEVFGNPRPATGFSADLLTLYQLSG
                     ASGPAGQGILVPDEDDDALAALVRRQRARGERVIVDLSGGASSAAEQQCNRIAVLDNG
                     TWTITEA"
     gene            complement(2211..2402)
                     /locus_tag="VIW27_00930"
     CDS             complement(2211..2402)
                     /locus_tag="VIW27_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY5738251.1"
                     /translation="MNWGDFWAALALVLVLEGLIPFISPGGYRNMVQQMSSMSDQVLR
                     NTGLALIITGVVFLYLVRG"
     gene            complement(2466..3338)
                     /gene="hflC"
                     /locus_tag="VIW27_00935"
     CDS             complement(2466..3338)
                     /gene="hflC"
                     /locus_tag="VIW27_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY5738252.1"
                     /translation="MTSNLRFGLILLAAAAAIVYMSTFIVHERELAIKFKLGEIVEAD
                     YQPGLYFQIPIINNILKFDSRILTMDTPSERFLTVEKKNVIVDSFVKWRIVDPKTFYT
                     ATRGDERQAVARMSSIITDELKGKIASRTMREVISGERAVIMEEVTANAAVKIRDLGI
                     QLVDVRVKKVELPDNVSNNVYQRMATERQTVAKEFRSRGEEQAKQIRANADRQREEIL
                     AEAYRKSEEIRGEGDAIAAKTYADAYNQDREFYSFYRSLKAYDSSFGNNQDMILLSPD
                     SDFFKYFKDSEAGR"
     gene            complement(3335..>3539)
                     /locus_tag="VIW27_00940"
     CDS             complement(3335..>3539)
                     /locus_tag="VIW27_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEY5738253.1"
                     /translation="MARSPKVMLDVEGGNNLMFLPLDQLMNRAATSSSGLKGLSGNSS
                     DSQSLIDEFTRNASRNRERESRQ"
BASE COUNT          647 a         1112 c         1080 g          700 t
ORIGIN      
        1 gagcttggtg atacacagac cgctgaccga gttgattttc agcgagcgtt tcagcgctac
       61 cagatcgagc cagccgcaac gccgtttgcg ccccgtggtg gcgccgaact cgtggccgcg
      121 gtcacccatg tgctggccac aggcgtcgtc gagctcggtc gggaacgggc ccgcgccgac
      181 acgggtcgtg tatgccttga cgataccgag gatgtagtcg atatcacgcg gaccgacacc
      241 agaaccggcc gccgcgccgc cggcggtggt ggtcgacgag gtcacgaacg gataagtgcc
      301 gtgatcgata tccagcaacg cgccctgcgc accctcgaac atcaccggtt cgccgcgctc
      361 gatcaggtcg tgcagtatcg tcgccacgtc gccgaccatg ccgctcacct gctcgccctg
      421 ttgcaggcat tcgtcgagca tctgctgaaa gtcgagcggc gagtcgccga aataatgctc
      481 gagcacgaag ttgtgataat cgagcagttc gccgagtcgc gccgaaaaat gtgcctcgtt
      541 ggccaggtcg ccgagacgca gtccgcggcg cgcgaccttg tcctcgtagc acgggccgat
      601 gccccggccg gtggtgccga tggcattgct gccgcgcgcc ttttcgcgtg cctggtcgag
      661 cgcaatatgc gatggcagga tcagcgggca ggcatcgctg acgacgatgc gctcgcgcgc
      721 cggcacaccg ctatcctcga gctgcgtcat ctccctgatc agtgcctcca gcgacaacac
      781 tacgccattg ccgatcaggc agttgacgcc gtcgcgcagc acacctgacg ggatcaggtg
      841 caataccgtc ttgttgccgt cgatgaccag cgtatggccg gcgttgtgcc cgccctgaaa
      901 gcgcaccacg gcattcgcgc ggtcggtcag cagatcgacg atcttgccct tgccctcgtc
      961 accccactgg gtaccgatta tgacgactgt atttgccatg agcccctgtc aagcctctgt
     1021 gatggtccag gtgccgttgt cgagcaccgc gatacgatta cattgttgtt ccgcggcact
     1081 gctcgcaccg ccgctcagat cgacgatcac gcgttcaccg cgggcccgtt gccggcgcac
     1141 cagcgcggcg agcgcatcgt cgtcctcgtc gggcaccagt ataccctgcc cggccggacc
     1201 gctggcgccg ctgagctggt aaagggtcag caagtcggcg ctgaagccgg tcgccggacg
     1261 ggggttgccg aagacttcgc cgacatcgtc ataacggcca ccgcgcgcga tttcccggcc
     1321 ctggccgggc aggaaggccg cgaacaccag cccggtgtga tagctgtagc cgcgcaattc
     1381 cgaaagatcg caatgaatcg acgtatcggg gtacatgcgg cgcagcgatt gtacgaccct
     1441 gctcatcgtg tccagcgtcg ccagtaccgc gtcgccggcg tcactgtaga ttccgcgcgc
     1501 ttcgtcgatc accgattcgt cgccgctcag caacgcgagc cgggaaatct gctcccgctg
     1561 ccggtcgtcg agcggcaact gctgcaggta gacctgcaga tcgggaatcg acttgcgctg
     1621 caacatgtcg aacaggcggt tttcctgctc gtcgtcgagc gaggtgtgcg cgaccaggcc
     1681 gcgaaagata ccgacgtgac cgatatccag cgacatctcg ccgaggccga ccgcgtgcag
     1741 ggcctcgacc atcaggctga cgatttcgag gtcggcgccg atcgactcgc tgccgaatat
     1801 ctcggcgccg atctgcagcg gcgtgcggct cgcgctgatg ccgtcgccga tcgcatgcag
     1861 caggtgcccg caatagcaca gccgcgacac gcgatgatga cgctcgtcgt cgaccagctg
     1921 gtgggcatcg atgcgcgcga cctgcggcgt catgtcggag cgcacgccca tctgcttgcc
     1981 ggaaagctgg tcgaccagcg cgaaggtgcg cgtatcgagc gcatgcgcgg taccggtgag
     2041 cagcgaatcc atatactcga tcatcgccgg catcaccagg tcgtagcccc agctgtgaaa
     2101 tatttgcaga atttcgtggc gcagcaactc gaccgcgagc gcatcgggcg gcagggtttc
     2161 ctgcacgccg tccggcaaca gccagcgctt ggtggatttc tcgttcaacg tcagccacgc
     2221 accaggtaaa gaaacacgac cccggtgatt ataagcgcga gacccgtatt tctcagcacc
     2281 tgatcggaca tggaagacat ttgctgcacc atgttcctgt agccgccggg gctgatgaaa
     2341 ggaatcaggc cctcgagtac caggaccagt gccagcgccg cccaaaaatc tccccagttc
     2401 atatgcttct cataaaaaac gggcgtgccc gggcgcccgt ttcgactttt ctgttagcct
     2461 gccggctagc gccctgcctc ggaatccttg aaatacttga agaagtcgga atccgggctc
     2521 agcaaaatca tgtcctggtt gttgccgaaa ctcgagtcgt aagccttgag actgcggtag
     2581 aacgagtaga attcacgatc ctggttgtag gcatcggcat aggtcttggc cgcgatcgca
     2641 tcgccttcac cgcggatttc ctccgattta cgataagcct cggcgaggat ttcctcgcgt
     2701 tggcgatcgg cgttggcgcg tatctgcttg gcctgctcct cgccgcgcga gcggaattcc
     2761 ttggccacgg tctggcgttc ggtcgccatg cgctggtaca cgttgttgct gacgttatcc
     2821 ggcaattcga cctttttgac ccgcacgtcg accagctgga tgccgaggtc ccgaatcttg
     2881 accgccgcat tggcagtgac ttcttccatg atcacggcgc gctcgccgga gatgacctcg
     2941 cgcatcgtac gcgaggcgat cttgcccttg agttcgtcag tgatgatcga cgacattctg
     3001 gccacggcct ggcgctcgtc gccgcgggtc gcggtgtaaa aggttttcgg atcgacgatg
     3061 cgccatttga cgaaggagtc gacgataacg tttttctttt ccaccgtcag gaatcgctcc
     3121 gacggcgtat ccatggtcag gatgcggctg tcgaacttga ggatgttgtt gatgatcggg
     3181 atctggaaat acagacccgg ctggtagtcg gcctcgacga tttcgccaag cttgaacttg
     3241 atcgccagtt cgcgctcgtg gacgataaac gtcgacatgt aaacgatcgc cgctgctgcg
     3301 gcgagcaaaa tgaggccaaa gcgaagatta ctggtcattg gcggctctcc cgttcgcggt
     3361 tacggctcgc gttgcgcgtg aattcgtcga tcagcgactg cgaatcgcta ctgtttccgc
     3421 taagaccctt gagcccgctg gagctggtcg cggcgcggtt catcagctgg tcgagcggca
     3481 ggaacatgag gttgttgccg ccctcgacat cgagcatgac cttgggcgag cgcgccatc
//
LOCUS       DATCZV010000040         3240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_161900, whole genome shotgun sequence.
ACCESSION   DATCZV010000040 DATCZV010000000
VERSION     DATCZV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161900"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VIW27_00945"
     CDS             <1..475
                     /locus_tag="VIW27_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY5738254.1"
                     /translation="SMRRVVGALLGSIPGLSSIALVLMLIYYVFAVIATKLFGASFPQ
                     WFGSIGESLFSLFQIMTLESWSMGIVRPVMELHPNAWIFFILFILIATFTMLNLFIAI
                     IVNAMHSFTEEENLERQQALDGVRDHIEADLHQEVTALRAEIAELRRLLAAGERN"
     gene            736..1290
                     /locus_tag="VIW27_00950"
     CDS             736..1290
                     /locus_tag="VIW27_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5738255.1"
                     /translation="MSNAKILRLNKAGTPIDWITREEAATLVVKDLVVWALGENAFEI
                     RGGINRSGRQSVLSLPSILASDGSVKLSDFVPPLVNGYLFRRDQYLCMYCGGEFGADN
                     LSRDHILPVSRKGTDSWTNVVTACKRCNHRKANQTPEEAGMQLLAVPFVPNRYEFFYL
                     ANKNILADQMEFLKSQFSKNGKWS"
     gene            1508..2008
                     /locus_tag="VIW27_00955"
     CDS             1508..2008
                     /locus_tag="VIW27_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738256.1"
                     /translation="MQDIFVDISKYYAARIVENVLTVGKIFYRDDEYQFAREDVAGPF
                     ELIEKQTDVTRIIHVIGEIHPRSAVERYFKVDVSSCLYLNENNETSEIEVRSIHKGIG
                     TSIYMHQQCPLLLWNPHYEEHLYFDVIDIQNVFADAGQKAAFVSILAESLREYQLFPP
                     LFSMKK"
     gene            complement(2113..>3240)
                     /locus_tag="VIW27_00960"
     CDS             complement(2113..>3240)
                     /locus_tag="VIW27_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5738257.1"
                     /translation="DLDSHVYRPSTLADLQDFTRLADRLPNIAWFTRCCVATDVADIL
                     DLDINTAYCLMRGTQKPVGTSITLGENVDPVVDLFDEVAGGAGKFAAKPFCKAHISPI
                     ISPMRYGADAFDVTLACIRRGMPINTIVAAMSGATSPATIDGMLAASFAETLAALVMV
                     NLFAPGFPVIFSNWPFVIDLRSGAFCGGGGEISLLNAAAAQLANHLGVPSGVASSMSD
                     AKAVDAQMGMEKALSSLACGLSGANLVYESSGMMASLLGASFEAFVLDDEMLSHVYRM
                     VRGIEVSEETLGFEAMRNVITGDGHFIGEPQTMQAMERDYFYPRLGDRLEPTAWAEAG
                     AEDAWQRANREVRSILASHRPDYIGKPGDARIRARFNILID"
BASE COUNT          679 a          919 c         1003 g          639 t
ORIGIN      
        1 gtcgatgcgc cgcgtggtcg gcgccctgct gggttcgatt ccgggcctca gttcgatcgc
       61 gctggtgctg atgctgatct actacgtgtt cgcggtgatc gctaccaagc tgttcggcgc
      121 gagctttccg caatggttcg gcagcatcgg cgaatcgctg ttttcgctgt tccagatcat
      181 gaccctggaa agctggtcga tgggtatcgt gcgcccggtg atggaactgc accccaatgc
      241 gtggatattt ttcatcctgt tcattctcat cgcgaccttt acgatgctca acctgttcat
      301 cgccatcatc gtcaatgcga tgcacagttt taccgaggag gaaaatctcg agcggcagca
      361 ggcgctggat ggcgtgcgcg atcatatcga ggcggacctg caccaggagg tcacagcgtt
      421 gcgcgcggag attgccgaat tgcggcgcct gctggcggcc ggcgagcgga actaggagcg
      481 cacccgggcg gcgcagcgtg cctaccaccg gtcgtgctgc aattgcgagc gccgagatag
      541 agtggtatat atcgctcgag aagggtcgtt atgcctgtgg gtcgacgcgt cacgccgcgg
      601 ttggaacccg ccttcattgc ctgttctgtc atcgaaccat aacccggcaa tgatacaagg
      661 cgctgttacc ggcaggcatg cggcggatat cgaatagaaa cgggttggag cgaaacagca
      721 gggcatggca ggaatatgag taacgccaag atactgaggc tcaacaaggc cggaacgccg
      781 atcgactgga tcacgcgcga ggaagccgcg acgctggtgg tcaaggatct ggtggtctgg
      841 gcgctcggtg aaaacgcgtt cgagattcgc ggtggcatca atcgcagcgg tcgccagtcg
      901 gtattgtccc tgcccagtat cctcgccagt gacggcagcg tgaagctgtc cgatttcgta
      961 ccgccgttgg tgaatggcta cctgtttcga cgcgatcagt acctgtgcat gtactgcggt
     1021 ggcgaattcg gcgcggataa cctgtcgcgc gatcatattc tgccggtttc gcgcaagggt
     1081 accgacagct ggaccaacgt ggttaccgcc tgtaagcgct gtaatcaccg caaggccaac
     1141 cagactcccg aagaagcggg catgcagtta ctcgcggtgc cgttcgtccc caaccgctat
     1201 gaatttttct atctcgctaa caagaacata cttgccgacc agatggaatt tttgaaatcc
     1261 cagttctcca aaaacggcaa gtggagctag gcccgcttcg cctgggttcg ggaactggcc
     1321 gataacgcct ggcgtaagcg ggcgtcgttg cgagtgcaga ccgcggggca gatcctggtc
     1381 ccggcaaggg tgcggagtgc agaaaaatcg aaactgtcaa aaggcagcga tcttctatac
     1441 ttcacgatat tatccgctcg agaataaagc tgtttagttg atctgctcat tgcggagttt
     1501 gagccaagtg caagacatat tcgtcgatat ctccaagtac tatgcggcgc gcatcgtgga
     1561 aaacgtgctc accgtcggta agatatttta ccgggatgac gagtaccagt tcgcgcgcga
     1621 ggatgtcgcc ggaccgttcg aactcatcga gaaacagacg gacgttacgc gcatcattca
     1681 cgtgatcggc gaaattcatc cgcgctcggc ggtggagcgg tacttcaaag tcgacgtgag
     1741 ttcgtgcctg tatctgaacg aaaacaacga aaccagcgaa atcgaagtgc gctcgatcca
     1801 caagggcatc ggcacgtcga tctacatgca ccagcaatgc ccgctgttgc tgtggaaccc
     1861 gcattacgag gaacacctgt atttcgacgt catcgatatt caaaatgtat tcgccgatgc
     1921 ggggcaaaaa gccgctttcg tatcgatact ggccgaaagc ctgcgcgaat accaactgtt
     1981 cccgccgttg ttcagcatga agaagtaacc gcggcggcct gcaaaatcct tacaacccgc
     2041 gggtcgcgac aacgccgaga tgcgagcgcg ccgtgtcgac aaggctcggg cgacgctgat
     2101 gtcgccggga tgtcaatcga tcaggatatt gaagcgcgcg cgaatacgcg catcgccggg
     2161 cttgccgata tagtcggggc ggtggctggc gaggatgctg cgaacctcgc ggttggcccg
     2221 ttgccaggcg tcctcggccc ccgcctcggc ccaggcggtg ggctctagcc ggtcgccgag
     2281 ccgcggataa aaataatcgc gctccatcgc ctgcatggtc tgcggctcgc cgataaagtg
     2341 accatcgccg gtaatcacgt tgcgcatcgc ttcgaagccc agcgtttcct cgctgacctc
     2401 gatgccgcga accatgcggt atacatgcga cagcatttca tcgtcgagca cgaaggcttc
     2461 gaatgaagcg ccgagcaggc ttgccatcat gccggaggat tcgtatacca ggttggcacc
     2521 ggagagcccg caagccagtg acgacagcgc cttttccatg cccatttgcg catccacggc
     2581 tttggcgtcc gacatgctcg aagcaacccc ggaaggcacg ccgaggtgat tggcgagttg
     2641 cgccgcggcg gcattgagca gcgaaatttc gccaccgcca ccacagaatg cgccgctgcg
     2701 cagatcgatc acgaacggcc agttggaaaa aatcaccggg aaacccggtg cgaacaggtt
     2761 gaccatgacc agcgccgcca gggtttccgc gaacgaggcc gcgagcatgc cgtcgatggt
     2821 ggccggcgag gtggcgcctg acatcgcggc aacgatggta ttgatcggca tgccgcgccg
     2881 aatgcaggcc agcgtcacgt cgaacgcatc cgcgccgtac cgcatcggcg agataatcgg
     2941 gctgatgtgc gccttgcaga agggctttgc agcaaacttg ccggcgccgc cggcgacctc
     3001 gtcgaacagg tcgacgaccg gatcgacgtt ttcgccgagg gtgatgctgg tgccgaccgg
     3061 cttttgcgtg ccgcgcatca ggcagtaggc ggtattgata tcgagatcga gaatgtcggc
     3121 gacgtcggtg gccacgcagc agcgagtgaa ccaggcgatg ttcggcaggc ggtcggcgag
     3181 gcgcgtgaaa tcctgcaggt cggccagtgt cgacggacgg taaacgtgac tgtcgaggtc
//
LOCUS       DATCZV010000041         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_426765, whole genome shotgun sequence.
ACCESSION   DATCZV010000041 DATCZV010000000
VERSION     DATCZV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426765"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /gene="argB"
                     /locus_tag="VIW27_00965"
     CDS             <1..299
                     /gene="argB"
                     /locus_tag="VIW27_00965"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5738258.1"
                     /translation="VLGAEKLLLLTNTPGVLDPDGKLLTGLDAARTEALIAEGVISGG
                     MLPKVRCALDAVRAGVRTAQIIDGRVRHSVLLELLTDSGVGTLIRGDDDGPLAP"
     gene            complement(296..961)
                     /locus_tag="VIW27_00970"
     CDS             complement(296..961)
                     /locus_tag="VIW27_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5738259.1"
                     /translation="MRNLYRPRNMLQNLFLVSLIYVFADPLASAGLYKWVDDDGMVRY
                     SDRLPPGQIRKSHSRLNERGLVVSTREAARPTEELAAEAEARRKQEEREAEEARVRAI
                     QQQKDQVLLMTFSSIEEIDLARDNRLEVLDSVINLIQGNIEHTQERLSHLQNRAENAY
                     ISQGMEVPGGLAQKIEHFERKVHTRSRQLELKLDERAKIISKYDSDLARFTELQGGTS
                     ASN"
     gene            complement(1081..1842)
                     /locus_tag="VIW27_00975"
     CDS             complement(1081..1842)
                     /locus_tag="VIW27_00975"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY5738260.1"
                     /translation="MARFLLLLSLLALSAEAAEPIVVNSGERRTAVVELYTSEGCSSC
                     PPADRWLSRLVETSGAEMQVLALSFHVDYWNYLGWRDRFSSADYTSRQRQLGANNRQQ
                     TIYTPEFFVDGKEARGSDNILDKIQQANAEQPPLPLQLRVSRDAQGLLLEVTTDGAFD
                     APLHHRYLVFENELSSHVLRGENAGRKLSHQRVVRYMSPALVLRAHNLHRIDIDPDWN
                     ADNIGVAVLVTSPGSLRYLDALQTSIAPLLGARKH"
     gene            complement(1901..>2158)
                     /gene="pyrE"
                     /locus_tag="VIW27_00980"
     CDS             complement(1901..>2158)
                     /gene="pyrE"
                     /locus_tag="VIW27_00980"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899993.1"
                     /note="involved in fifth step of pyrimidine biosynthesis;
                     converts orotidine 5'-phosphate and diphosphate to orotate
                     and 5-phospho-alpha-D-ribose 1-diphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY5738261.1"
                     /translation="TAGTAIRESIALIEALGAQVTAVTIALDRQEKGLGDTSAIQELE
                     RDGLAVISIIKLDHILEYLAASGAAGQRDAIEAYRAQYGID"
BASE COUNT          359 a          641 c          696 g          462 t
ORIGIN      
        1 gtgtgctggg cgccgaaaag ctgttgctgc tgaccaatac gccgggcgtg ctcgatccgg
       61 acggcaagtt gctgactggc ctggacgcag ctcgcaccga ggcattgatc gccgaaggcg
      121 tgatctcggg cggcatgctg ccgaaggttc gctgcgcgct ggacgcggtg cgcgccggcg
      181 tacgtaccgc gcaaatcatc gatggccggg ttcggcactc ggtattgctg gaattgctaa
      241 cggatagcgg cgtgggtacg ctgatccgtg gcgacgacga cggaccgctg gcgccctagt
      301 tgctggcgct ggtcccgccc tgcagttccg tgaagcgggc caggtcggaa tcgtacttgg
      361 aaattatttt cgccctttcg tcgagcttaa gctcgagttg ccgggagcga gtgtggacct
      421 tgcgctcgaa atgctcgatc ttctgtgcca gcccgccggg gacttccatg ccctgtgaaa
      481 tataggcatt ttccgcgcgg ttctgcaggt gcgaaagtct ttcctgcgta tgctcgatat
      541 taccctggat caggttgatc accgagtcga gtacctcgag gcgattgtcg cgcgcgagat
      601 cgatttcctc gatcgagctg aaggtcatca acagcacctg gtccttctgt tgctgtatcg
      661 cccgcacccg tgcttcctcg gcttcccttt cctcctgctt gcgccgtgcc tcggcctcgg
      721 cagcgagctc ctctgtgggg cgggcggcct cgcgggtgga gaccaccagg ccgcgctcgt
      781 tgagccggct gtgcgatttc ctgatctggc ccggcggcag gcggtcgctg tagcgaacca
      841 taccgtcatc atcgacccac ttgtagagtc cggccgacgc cagcgggtcg gcgaaaacgt
      901 aaatgagcga caccagaaac aggttctgta gcatgttacg cgggcggtaa agattcctca
      961 tgacgactcg cagggcctca attttccatg tttttccagc agttagacga actgtctata
     1021 ggataatagg ctatgaattg cgagttttca gaggtttgcg cgctccgttc gtcggaaatt
     1081 ttagtgcttc cgggcgccta gcagcggggc gatcgaagtc tgtaatgcat cgagataacg
     1141 caagctgccg ggcgaggtga ccagcaccgc gacgccgatg ttgtcggcgt tccagtcggg
     1201 atcgatgtcg atgcggtgga ggttgtgtgc cctcagcacc agtgccgggc tcatgtagcg
     1261 caccacccgc tgatggctga gtttcctgcc ggcgttctcg ccacgcagta catggctcga
     1321 caattcgttt tcgaacacca ggtagcggtg gtgcaggggg gcgtcgaaag cgccatccgt
     1381 cgtgacttcg agcagcaggc cctgcgcgtc tcgcgatacc cgcagttgca gcggcaacgg
     1441 tggctgttcg gcattggcct gctgaatttt atcgagaata ttgtcgctgc cgcgggcttc
     1501 cttgccgtcg acgaagaatt ccggcgtgta aatcgtctgc tgccggttgt tggcgccgag
     1561 ttgtcgctgc cggctggtat agtcggcgct cgaaaagcgg tccctccagc cgaggtaatt
     1621 ccagtaatcg acatggaacg acagcgccag cacctgcatt tcggcgccgg atgtctccac
     1681 cagccgcgac agccagcgat cggccggcgg gcagctgctg caaccttccg aggtatagag
     1741 ttcgaccacc gcggtgcgtc gctcgccgct gttgacgacg attggctcgg cggcctcggc
     1801 ggagagcgcc agcaggctca gaagcagcag gaatcgggcc atggggcacc tgtttgtcga
     1861 ctcggctcga gactggtaga ccgggccgcg gtcgccaagt tcaatcgata ccgtattgcg
     1921 cccggtaagc ctcgatcgcg tcgcgctggc cggcggcgcc gctggcggcg aggtattcga
     1981 ggatatggtc gagcttgatg atcgatatta ccgccagccc gtcgcgttca agctcctgta
     2041 tcgccgaggt gtccccgagg cctttttcct gccggtcgag cgcgatcgtc accgccgtga
     2101 cctgcgcgcc gagcgcttcg atcagcgcga tcgactcgcg aatcgcggtg ccggcggt
//
LOCUS       DATCZV010000042         3175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_373715, whole genome shotgun sequence.
ACCESSION   DATCZV010000042 DATCZV010000000
VERSION     DATCZV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373715"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..357)
                     /locus_tag="VIW27_00985"
     CDS             complement(106..357)
                     /locus_tag="VIW27_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738262.1"
                     /translation="MNTAKKTTGIAMAAAAAAMFAVAPMSASAGETTGKCFNVNSCKG
                     TGACATATTSCAGQNSCAGQGWVKRTKAECEDAGGKFEA"
     gene            complement(502..1017)
                     /locus_tag="VIW27_00990"
     CDS             complement(502..1017)
                     /locus_tag="VIW27_00990"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY5738263.1"
                     /translation="MAHSTKKLGMMFTTAAWVLGFLLLALVFSKILDGQRNPNQSVST
                     LDTGEYREIVLKRSRNGHYLFDGEANRRKVTFLVDTGATMTAIPGKMQHDLGLQAGPA
                     MSVSTANGVASAKLTRLEQLAIGGIELYDVDATLIDDMAIDEILLGMNVLRHFELVQR
                     GDQLIIRQYRQ"
     gene            1070..2170
                     /gene="aroC"
                     /locus_tag="VIW27_00995"
     CDS             1070..2170
                     /gene="aroC"
                     /locus_tag="VIW27_00995"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY5738264.1"
                     /translation="MSGNSFGKSFVVTSFGESHGGAIGCVVDGCPPGLELDESDLQHD
                     LDRRKPGTSRHTTQRREDDSVQILSGTFEGRTTGTPICLLVYNKDQRSKDYSEIMNLF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGGIAKKYLREKCGIEVRGYLAQL
                     GPLKPVALDWQQVDRNPFFCPDAELVPQLEKYMDALRKEGESIGARVTVVASGVPPGL
                     GEPIFDRIDADIAHAMMSINAVKGVEIGAGFGSIELKGTQNRDEITPEGFLSNHAGGT
                     LGGITSGQDVVVSIALKPTSSIHLAGRTVDIDGNAVEIRTKGRHDPCVGIRATPIAEA
                     MLAIVLMDHYLRNRAQNADVDSRTPVIPASPD"
     gene            complement(2660..>3175)
                     /gene="yjgA"
                     /locus_tag="VIW27_01000"
     CDS             complement(2660..>3175)
                     /gene="yjgA"
                     /locus_tag="VIW27_01000"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY5738265.1"
                     /translation="TQNEADFEEIEYVSKSQLKRDSLALQALGKQLAALPPEHLARIP
                     LDAPVLEAIALAKRIQNKRSALKRHYQFLGKLLRARDPEPIRAALAEIEREAGDSIRR
                     HHRAEHWRDRILDDGNAAIDALLDETPGADRQKLRQLYRNYEKAPDDSKRTQHARLVY
                     KEVFQALGSEN"
BASE COUNT          616 a          930 c          979 g          650 t
ORIGIN      
        1 cgggactcac atcatgcctc cagccgggga cggtttttat tgtagcgagc gggcaaaaaa
       61 atgcaccggg caagccggtg cattttgatg gctgagaaat acagctcagg cttcgaactt
      121 gccgcctgca tcttcacact cggccttggt gcgcttgacc catccctgac cggcgcagga
      181 gttctgaccg gcgcagctgg tggtggcagt ggcgcaggca ccggtgccct tgcaggaatt
      241 gacattgaag catttgccgg tggtttcgcc ggccgaagcg ctcatcggcg cgacagcgaa
      301 catggctgcg gcggctgctg ccattgcgat cccggtggtt ttcttggctg tattcataaa
      361 tacgtctcct cgataattga tcggatgcct gtagatggtt tgttccccat tacgcgagct
      421 tagaggagcg gcttttgcgg aaagttcaaa aaattctgca aattgtcgga aagtgcgaat
      481 ttttttaccg gccctggttt gtcactggcg atactggcga atgatcagtt ggtcgccgcg
      541 ctgtaccagc tcgaaatgtc gtaacacgtt catgccgagc aggatttcgt cgatggccat
      601 gtcgtcgatc aacgtggcgt cgacgtcgta cagctcgatg ccgccgatgg cgagctgttc
      661 gagccgggta agctttgcgc tggcgacgcc gttggcggtc gagaccgaca tcgccgggcc
      721 cgcctgcagt ccgaggtcgt gttgcatctt gccgggaatc gcggtcatcg tcgcgccggt
      781 atcgaccagg aaagtgacct tgcggcggtt agcctcgccg tcgaacaggt agtgcccgtt
      841 gcgactgcgc ttgagcacga tctcgcggta ttcaccggta tccagcgtgc taaccgattg
      901 gttcggattg cgctgaccgt cgagaatctt tgaaaatacc agcgccaaca ataaaaaacc
      961 gagcacccag gccgcagtag tgaacatcat gcccagtttt ttagtagaat gcgccatagc
     1021 actgtaacgt agtgatattc ctgtcaattg caatctcaag ggccgtttga tgtctggcaa
     1081 cagtttcggc aaatccttcg tcgtgacctc gttcggcgag agccacggcg gcgccatcgg
     1141 ctgtgtcgtc gacggttgcc cgccgggact tgaactcgac gagagcgacc tgcagcacga
     1201 tctcgaccgc cgcaagcccg gcacctcgcg gcataccacc cagcgccgcg aggacgatag
     1261 cgtgcagatc ctgtcgggca ccttcgaagg tcgtaccacc ggcacgccga tctgtctgct
     1321 ggtgtacaac aaggatcagc gctcgaagga ttacagcgaa atcatgaacc tgtttcggcc
     1381 gggtcatgcc gattacacct accagcagaa atacggcatt cgcgactacc gcggcggcgg
     1441 gcggtcgtcg gcgcgcgaga ccgcgatgcg cgtggctgcc ggcggcattg ccaagaagta
     1501 cctgcgcgag aagtgcggca tcgaggtgcg cggctacctg gcgcagctgg ggccgttgaa
     1561 gccggtcgcg ttggattggc agcaggtcga tcgcaatccg ttcttctgcc ccgacgccga
     1621 actggtgccg cagctggaga aatacatgga tgcgctgcgc aaagaaggcg aatccatcgg
     1681 tgcccgcgtc acggtcgtcg ccagcggtgt gccaccggga ctgggcgagc cgatcttcga
     1741 tcgtatcgat gccgatatcg cgcacgcgat gatgagcatc aacgcggtca agggcgtcga
     1801 gatcggtgct ggcttcggca gcatcgaact caagggtacc cagaaccgcg acgaaatcac
     1861 gcccgagggg ttcctgtcga accatgccgg cggcacgctc ggcggtatta cctccggcca
     1921 ggatgtggtc gtcagtatcg cgctgaaacc gacctcgagc attcacctgg cgggacgcac
     1981 ggtcgacatc gacggcaatg ccgtcgagat ccgtaccaag ggccgccacg atccctgcgt
     2041 cggcattcgc gccacgccga tcgccgaggc gatgctggcg atcgtgctga tggatcatta
     2101 cctgcgcaac cgcgcgcaaa acgccgacgt cgacagccgc accccggtga ttcccgccag
     2161 ccccgactga caccgggaaa cccggggtca gatcaaaaat tccgcgaact ccccggctaa
     2221 cgcctgcgcc ggttggcgaa agccctgcct gcgcagggat ttcgtcacgc ttcgatcgaa
     2281 gcagcatcga ccggggaatt tttgctctga cccccggcgg agcgacgggt gtcgaataat
     2341 cgatgccagt ggcgcaagcc cgtttaagcg ggcttgccga agccggcggt tgagcgaata
     2401 gccagacggc tgatatcgat tcagtaaact ggggaattct tcgttcaagc ctggatgatc
     2461 ccagcttttg aaagcatttt tccggaacgt cgctccagcc aaaaaagttg caaggatcag
     2521 cctggggcga ccttcggtcg aagcagggga ggcttacaga tgctttgctc ccgtcgagga
     2581 ggacaggtca gctgacggta acgaatgagg caacagttcg acgtcggcga cagttgtaca
     2641 ctcatcaagt ctccagtaac tagttctcgc tccccagtgc ctgaaatacc tccttgtaaa
     2701 cgagccgcgc atgctgagtc cgcttgctgt catcgggcgc cttctcgtaa ttgcgatata
     2761 actgccgcag tttctgccga tcggctccgg gagtctcgtc gagcaacgcg tcgatggcgg
     2821 cattgccgtc gtcgaggata cggtcgcgcc aatgctcggc gcgatggtgg cggcgaatcg
     2881 aatcgccggc ttcgcgctcg atctcggcga gcgcggcgcg gatcggctcg ggatcgcgcg
     2941 cgcgcagcaa tttgccgaga aactggtagt gccgcttgag agcgctgcgc ttgttttgaa
     3001 tgcgcttcgc cagtgcgatc gcttcgagca ccggcgcatc cagcggtatg cgggcgaggt
     3061 gctccggggg cagtgcggcc agctgcttgc cgagcgcctg caatgccagg ctgtcgcgtt
     3121 tcagctgact cttgctgaca tattcgattt cttcaaagtc tgcttcgttt tgcgt
//
LOCUS       DATCZV010000043         5181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_214860, whole genome shotgun sequence.
ACCESSION   DATCZV010000043 DATCZV010000000
VERSION     DATCZV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214860"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1958
                     /locus_tag="VIW27_01005"
     CDS             33..1958
                     /locus_tag="VIW27_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY5738266.1"
                     /translation="MNQQSPPELTPRAIVLAIVLAVILAAANAYLGLFAGMTIASAIP
                     AAVVSMAILKLLGGGHILENNIVATGASAGTSIATGVIFTIPALILLEYWTDFRYMWV
                     LAIAGLGGLLGVLFSVPLRRSLIVDQGLAFPEGKAAAEVLRTGDRPGEGLKILAGAAA
                     LGGAIKLAAGSGMRLIPDTAGVAAWFDKTVGYFGTNLSAALFGVGYIVGLNIGVVMLA
                     GGAIAWVVAIPLYSTYFLAADPVLSVQLAGASAEDAAYAIWSTQIRYLGVGAMLTGGV
                     WTLISLRKSLISGIRSGLSAERGAAGEVVPATERDLPMRHILIGTVLFVLPLLLLYQA
                     IVGKWWVSIPMTIIMIVAGFLFVSVSAYMAGLVGSSNNPVSGLTIATILFAALVLAVL
                     LGRDSPIGAVATIMIGAVVCCAACIGGDNLQDLKCGYILGATPWKQQVMLAVGGVSSA
                     FIMAPVLSLLAQAYGIGVPTAEHPNPLLAPQANLMASVAKGIFGGELPWTMIGIGALI
                     GAAIIIVDEILKKSGAAWRAPVLACAVGIYLPIELSVPIFAGGLVAHLVERKFAPGEA
                     EGDRERLHQKGTLFSAGLITGEALMGIIIAIPIVVLESADVLALPEAMHFGGWLGLAM
                     LTTVGVFLYRTAIRQTA"
     gene            1966..2985
                     /locus_tag="VIW27_01010"
     CDS             1966..2985
                     /locus_tag="VIW27_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5738267.1"
                     /translation="MAAQHGIRWGFVGTGAIADSMATVVGMAPSATLAAVASRTMASG
                     SDFARRHGIDLVFDDWAELVACDAVDAVYVATPTSAREAICIGAANHGRHVLAEKPLA
                     SLASLRRIIAACRGSGVAFMDATHFVHHPRTADIRATMATRVGWPWSIDSAFQFYLAD
                     RHNIRFDPRLEPLGAIGDAGWYNMRAAVEYLAPDIELREVNAYLRRDEVSGAVIGGSG
                     ILTFDDGATSTWNCGFDSGAVISDLRISGERGSIGVSDFLGQDPDGSASYTWQHRGRD
                     PDAAVDSVRIASSLPGAARMFEDFARLVLDPALREPSMRATERTQAWLDAAWDSALCN
                     ERAAG"
     gene            complement(3156..>5181)
                     /locus_tag="VIW27_01015"
     CDS             complement(3156..>5181)
                     /locus_tag="VIW27_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738268.1"
                     /translation="TAVAAEPAWMADAIATLEQELVTTHGIEQRARVTQGLDQVANFW
                     RDEDGDRAAFETFVRRNFAGDEKSLGVMFDRFERLLEKLDGHSLEILLAFREQSDLDR
                     GPIEAYDEIFAAYNPFAHITDDFFANKLAFVALLNFPVTSLAQQLDAGQNWSRADWAR
                     TRLADRFAKRVPADVQQAVAVAGAEADQYIAEYNIWMHHLVNDRGERLFPAGMKLLSH
                     WNLRDEIKANYSAGDATGLTKQRMVQKVMERIVDQTIPAVVVNNPHVDWNPYSNEVSA
                     AAVTDVAEPAPAGLVVSSAPEPDTRYAVLLGTYRAVRQVDPYSPTAPTHIDRSFNESR
                     QIPEARVVAMLETVLSSPLLKRTGELIESRLGRPLEPFDIWYNGFRARGAYSEADLDA
                     ITTKRFPTAAAYEAEIPKLLVGLGFEASRAAELAANIVVDPARGSGHAWGASMREAKS
                     HLRTRVGPSGMDYKGFNIAVHEMGHNVEQTISLNDIDHYLLQGVPNTAFTEALAFVFQ
                     GRDLELLGLASPSAEARALNTLDDFWGAAEIAAVGLVDIAVWHWMYDNPEATPAELKA
                     ATLQISKDVWNRYFAPVFGARDVTLLGIYSHMIHSFLYLPDYPVGQMIASQIEAQIDK
                     AGDLGAEFERMCLAGNIAPDLWMQNATGRPVGPEALLQAAERALEVVGSK"
BASE COUNT          827 a         1722 c         1680 g          952 t
ORIGIN      
        1 cataattcca acagccggcc aggagtctgc cgatgaacca gcaatcgccc cccgaactga
       61 caccgcgcgc catcgtgctc gccatcgtgc ttgccgtgat actcgcagcg gccaacgcgt
      121 acctcggact gttcgccggc atgacgatcg cgtccgcaat ccctgccgcg gtcgtgtcga
      181 tggcgatact gaaactgctc ggcggcgggc acatcctcga gaacaacatc gtcgcgacgg
      241 gcgcgtcggc cggcacgtcg atcgcgacgg gcgtcatctt cacgataccc gcgctgatcc
      301 tgctcgaata ctggacggac ttccgctaca tgtgggtcct cgcgatcgcc ggtctcggcg
      361 gattgctcgg cgtccttttt tcggtgccgc tccggcgctc gctgatcgtc gaccagggtc
      421 tcgcattccc cgaaggcaag gcagctgcgg aagtgctgcg cacgggcgac cggcccggcg
      481 aaggattgaa aatactcgcc ggcgcggcgg cgctcggcgg cgcgatcaag ctcgccgcgg
      541 gcagcggcat gcgcctgatt ccggacacgg caggcgtcgc ggcctggttc gacaaaacgg
      601 ttggctactt cggcacgaac ctgtcggccg ccctgttcgg cgtcggctac atcgtcggcc
      661 tcaatatcgg cgtcgtcatg ctggcgggtg gcgcgatcgc ctgggtcgtc gcgattccac
      721 tctacagcac ctattttctt gcggccgacc cggtcctgag cgtgcaactc gccggcgcaa
      781 gtgccgagga cgcggcctat gcaatctggt cgacccagat tcgctacctg ggtgtcggtg
      841 cgatgctgac cggtggtgtg tggaccctga tttcgctgcg caaatcgctg atttcgggca
      901 tccggagcgg gctgtccgcc gagcgtggcg ccgcgggcga agtcgtcccg gcgaccgagc
      961 gtgacctgcc catgcgccac atcctgatcg gcacggtgct gttcgtgttg ccgctcctgc
     1021 tgctctacca ggccatcgtc ggcaagtggt gggtcagcat tccgatgacg atcatcatga
     1081 tcgtcgcggg cttcctgttc gtctccgtat ccgcgtacat ggccggtctc gtgggctcat
     1141 cgaacaaccc ggtgtccggg ctgacgatcg cgacgatcct gttcgcggca ctcgtgctcg
     1201 ccgtcctgct cggccgtgat tcaccgatcg gcgccgtggc gaccatcatg atcggcgccg
     1261 tcgtctgttg tgcggcgtgt atcggcggtg acaacctgca ggatctcaag tgcggttaca
     1321 tactcggcgc cacgccatgg aaacagcagg tgatgctcgc ggtcggcggc gtatcgagtg
     1381 cattcatcat ggcgccggtg ctgagcctgc tggcacaggc ctatggcatc ggcgtgccga
     1441 ccgccgaaca cccgaatccg ctgctcgcgc cgcaggcgaa cctcatggcc tcggtggcaa
     1501 aaggcatctt cggcggcgaa ctgccgtgga cgatgatcgg tatcggcgcg ctgatcggcg
     1561 cggccatcat catcgttgac gaaattctca agaaatccgg cgccgcctgg cgcgcgccgg
     1621 tactggcctg cgccgtcggc atctacctgc cgatcgaatt aagcgtgccg atcttcgccg
     1681 gtggcctggt cgctcacctc gtcgaacgca agtttgcgcc cggcgaggcg gaaggcgatc
     1741 gcgaacgcct gcaccagaag ggcacgctgt tctcggccgg cctcattacg ggcgaagcgc
     1801 tgatgggcat catcatcgcg attccgatcg tcgtactcga gtcggcggac gtgctcgcgc
     1861 tgcccgaggc gatgcatttc ggtggctggc tcggactcgc gatgctgacc acggtcggcg
     1921 tattcctgta caggacggcg atccggcaaa ccgcgtaatt cgaccgtggc ggcacaacac
     1981 ggtattcgct ggggctttgt cggcacaggc gccatcgcgg acagcatggc gaccgtcgtc
     2041 ggcatggcac catcagccac gctggccgcg gtcgcgagcc gaacgatggc ttcgggctcc
     2101 gactttgccc ggcgacatgg catcgacctg gtcttcgacg actgggcgga gctcgtcgcc
     2161 tgcgacgcag tggatgccgt ttatgtcgcg acaccgacca gcgccaggga ggcgatctgt
     2221 atcggcgccg cgaatcacgg caggcatgtg ctcgcggaga aaccccttgc cagcctcgca
     2281 tcgctgcggc gcatcatcgc ggcctgccgt ggcagcgggg tggctttcat ggacgccacg
     2341 catttcgtcc atcacccgag gaccgccgac atcagggcga cgatggcgac aagggtcggc
     2401 tggccgtggt cgatcgattc cgccttccag ttttacctcg cggatcgcca caacattcgc
     2461 ttcgacccca ggctcgagcc cctcggggcg atcggcgatg cgggctggta caacatgcgc
     2521 gcggccgtcg aatacctcgc acccgatatc gaacttcgtg aggtcaacgc ttacctgcgc
     2581 cgtgacgaag tgagcggcgc ggtgatcggc ggcagcggca tcctgacctt cgacgatggc
     2641 gccacgtcca cctggaactg cggattcgat tccggcgccg tgatttcgga cctgcgcatc
     2701 tcgggcgaac ggggttcgat cggcgtcagc gatttcctgg gccaggatcc cgacggctcg
     2761 gcaagctaca cctggcaaca tcgtggccgc gatccggacg ccgccgtcga cagcgtgcgc
     2821 atcgcctcat ccctgcccgg tgcggcacgc atgttcgagg acttcgcgcg gctggtcctc
     2881 gacccggcgt tgcgcgaacc cagcatgcgc gccaccgagc ggacccaggc atggctcgac
     2941 gcggcctggg acagcgccct ctgcaacgag cgcgcagcgg gctgacggcc gtcgattccg
     3001 cgtctcgcga gcgtatcgcc gctcgggcac ggcgcggacc tgtgccgtcg cgggggcagc
     3061 gccgcaactg atagcaagac cccgatgctg cggcccgcga ccccggcgcg atgctgccat
     3121 cgtggcctgg cgggccactc gcccgccgat tttctttact tcgaaccaac cacctcgagt
     3181 gcccgctccg ccgcctgcag cagggcctcc ggcccgaccg gccgccccgt ggcgttctgc
     3241 atccagaggt cgggcgcgat attgccggcg aggcacatgc gctcgaactc ggcgccaaga
     3301 tcgccggcct tatcgatctg cgcctcgatc tggctcgcga tcatctggcc gaccgggtag
     3361 tcgggcaggt acaggaacga atgaatcatg tgcgagtaaa tgccgagcag cgtgacgtcc
     3421 ctggcgccaa agaccggcgc gaaatagcga ttccagacgt ccttcgaaat ttgcagcgtt
     3481 gccgccttca gctcggccgg cgttgcctcg gggttgtcat acatccagtg ccagaccgcg
     3541 atatcgacga ggccaacggc cgctatttcg gccgcgcccc agaaatcgtc cagcgtgttc
     3601 agcgccctgg cttccgcgct cggtgacgcc aggcccagca gttcgaggtc gcgtccctgg
     3661 aacacgaagg ccagcgcttc cgtgaatgcc gtgttgggaa cgccctgcag caggtagtga
     3721 tcgatgtcgt tcagtgaaat cgtctgctcg acgttgtgac ccatctcgtg gaccgcgatg
     3781 ttgaagccct tgtagtccat gccactgggc ccgacgcgcg tgcgcaggtg cgacttcgcc
     3841 tcacgcatcg acgcgcccca ggcatggccc gaaccccggg caggatcgac gacgatgttc
     3901 gcggccagct cggccgcgcg agacgcctcg aacccgagtc cgaccagcag cttcgggatc
     3961 tccgcctcgt aggcggccgc cgtgggaaaa cgcttggtcg tgatcgcgtc gaggtccgct
     4021 tcgctgtagg cgccgcgtgc gcggaagccg ttgtaccaga tgtcgaatgg ctcgagtggc
     4081 ctgccgagcc gcgactcgat cagttcgccc gtgcgcttga gcagcggcga cgacagcacg
     4141 gtttcgagca tggcaaccac gcgcgcctcg ggaatctgcc ggctttcgtt gaagctgcga
     4201 tcgatgtgtg tcggcgcggt cggcgaatac ggatcgacct gccgcaccgc gcggtaagtg
     4261 ccgagcagaa ccgcgtagcg ggtatcgggc tccggcgcgg aagacaccac cagcccggcc
     4321 ggcgccggct cggcgacatc cgtcacggcc gctgcagaga cttcattgct gtacgggttc
     4381 cagtcgacgt gcggattatt gacgaccacc gcgggaatgg tctggtcgac gatgcgctcc
     4441 atgaccttct gcaccatccg ctgcttcgtg aggccggtcg cgtcgccggc gctgtagttg
     4501 gccttgatct cgtcgcgcag gttccagtgc gaaagcagct tcatgcctgc cggaaacagc
     4561 cgctcgccgc ggtcgttgac gagatgatgc atccagatgt tgtactcggc gatgtactgg
     4621 tccgcctccg caccggcaac ggccaccgcc tgttgtacgt ctgccggcac gcgtttcgca
     4681 aagcgatcgg caagacgcgt tctcgcccag tccgcgcggc tccagttctg ccctgcgtcg
     4741 agttgctgcg cgagcgaggt gacggggaaa ttgagtaacg ccacgaatgc aagcttgttg
     4801 gcgaagaaat cgtcggtgat gtgcgcgaac gggttatagg ccgcaaagat ttcgtcgtag
     4861 gcttcgatgg gaccgcggtc caggtccgac tgttcgcgaa acgcgagcag gatttcgagc
     4921 gagtgaccgt ccagcttttc gagcagtcgc tcgaagcggt cgaacatgac gccgagcgac
     4981 ttttcgtcgc ccgcgaaatt gcgccgcacg aacgtttcga acgcggcgcg atcgccgtct
     5041 tcgtcccgcc agaagttcgc gacctgatcc agtccctgcg tgaccctggc gcgctgctcg
     5101 ataccgtggg tggtaacgag ttcctgctcc agcgtcgcaa tggcgtcggc catccaggcc
     5161 ggctccgccg cgacggccgt t
//
LOCUS       DATCZV010000044         7679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_426860, whole genome shotgun sequence.
ACCESSION   DATCZV010000044 DATCZV010000000
VERSION     DATCZV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426860"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VIW27_01020"
     CDS             complement(<1..460)
                     /locus_tag="VIW27_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEY5738269.1"
                     /translation="MPAADATTEHEQVYRAAAYSLLAALLRAPPDRALLEHVARLSPG
                     SDAEPDELLEAMRQLSASAAKSSLEQIDDEYHALFIGIGRGELVPYGSWYLTGFLMEQ
                     PLSELRDDLRALGFERSPDTHEPEDHISAIFEVFSVIIADGAGHEFQQQFF"
     gene            complement(488..2053)
                     /locus_tag="VIW27_01025"
     CDS             complement(488..2053)
                     /locus_tag="VIW27_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5738270.1"
                     /translation="MSNSDARQHALEARDRAPLEATDLVSYGSAGRILAVGDDAALEH
                     CDRLPPGLDIERVAAGADSVSIDGYLGAFTVGVTDAAGNTAAHRADAVLDLNSRPLLA
                     REMPPPGYLHAPPEEWDSLDTVAELENLSGEFEKPRYFDYDASICAHSVNGQTVCSRC
                     IDACPAEAIVSIGERIEVNPNLCQGGGSCATVCPSGAIRYRYPNLRDNGKRLRDMLAA
                     FREHGGRQPIVMFHAETCAPDAYLEAYDDLLPVAVEELGSIGMDLCLSAFAYGASQVL
                     LLVDDAVPASTRDNLRAQLDWLHALIAPLGMGSAHACLLAADAPLPTLDSSPDLEPSV
                     LDMPLLKRSAIYQALDHLAGQLRPASGEVELPPGAPFGEALIDAGKCTLCMACVGACP
                     GRALQDGSNRELPEVLFVEGNCLQCGACVQTCPEDAITLRPRLLFDRERRNRARALNS
                     DAPFACIACGKPFAPTSVINKMQDKLRDHYMFGSRRALDRLKMCENCRVADIVQDPEA
                     MGGQFDPLKGFRQ"
     gene            complement(2050..2667)
                     /locus_tag="VIW27_01030"
     CDS             complement(2050..2667)
                     /locus_tag="VIW27_01030"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEY5738271.1"
                     /translation="MTADSKQSGRGDESALSRWSRRKLEARQQQAEPRRPSSAAMTED
                     RPAPAEQAVERAAEAEKPLLTDADMPAIDSLDENSDFSQFMSAGVSDELRNLALRKLF
                     HAPAFNIRDGLDEYDDDFTSFEKLGDIVTSDMKHRIEMEQQKLRDQLAEEQAADDAVE
                     SESAELDAVEDIDGAEDEARDDAEPDADQMARLETAGETGAGDAR"
     gene            complement(2664..3200)
                     /locus_tag="VIW27_01035"
     CDS             complement(2664..3200)
                     /locus_tag="VIW27_01035"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEY5738272.1"
                     /translation="MAKLELDDFDQELPGHMNVAVMMEKQPSTHPWADFRYQALGVVV
                     RGPDDEYRVERVFVDGDIEHFLVTGLRVQLYEDECESYYHNLMSPEPGCFVVANRPDS
                     DAEMPEPYLVSLSFDEVHAYGEGDEEVYAVPIPPELYRWAEAYVLTHYVAVKKTKRKL
                     RNWRDPQAADGPPRGEQS"
     gene            3199..3594
                     /locus_tag="VIW27_01040"
     CDS             3199..3594
                     /locus_tag="VIW27_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738273.1"
                     /translation="MDLQRYRDSGICSYTQLGHCPATSLFSGINAWPAQIGFPNLAIR
                     EIDSFGKPARTGLHREHPWIEIAIEGIRVKTPRSTQFRATFDEIFAQAKISPTRAAVS
                     KGFIGIFAPTPIVRCPEALRRMELQFWQD"
     gene            complement(3788..4612)
                     /locus_tag="VIW27_01045"
     CDS             complement(3788..4612)
                     /locus_tag="VIW27_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5738274.1"
                     /translation="MTEIRVACVQNTAGRDIHANVERVCAQIDEAVAGGADFVALPET
                     VGLIEPVNERIPPATYGEVDDIGLAAFRGRARQHGVWILVGSQLIRDDDRIHNRSFLL
                     DEQGEIRARYDKLHMFDIELRNGEAYRESDAIAPGGRAVVVDTPLGRIGLSVCYDLRF
                     GALYRALAHGGAQIITIPAAFTQTTGEAHWHVLVRARAIETGAYVLAPNQCGHHADKR
                     YSYGHSLIVDPWGEVLADGGAEPGVVFADIDLGKVAKVRQRIPVLDNERDFTLEVT"
     gene            complement(4605..5726)
                     /gene="ald"
                     /locus_tag="VIW27_01050"
     CDS             complement(4605..5726)
                     /gene="ald"
                     /locus_tag="VIW27_01050"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY5738275.1"
                     /translation="MLIGVPKEIKNHEYRVGMAPASVREAIKHGHELMVETLAGDGIG
                     VSDDDYRQVGAHIVDTPEEIFAEAELVVKVKEPQAVERAMLREGQVLFTYLHLAPDPE
                     QTRELVDSGATCIAYETVTSPHGGLPLLAPMSKVAGRMAIQAGAYCLEHPHGGLGMLL
                     GGVPGVDPAKVVILGAGVVGTHATHIAVGMGADIWVIDRNAEVLEAHWKQFGRSTNTV
                     FSTEDAIERHVIDADLVIGGVLSPGAEAPKLVTAEMVRNMKPGAVIVDVAIDQGGCCE
                     TSKPTTHADPTYIVDDVVHYCVANMPGGVPRTSTYALNNVTLPHVLALADKGYRRALM
                     EDQWLRDGLNVHKGKVTQREVAHDLGYDYFAAEEVLRHD"
     gene            5864..6874
                     /gene="moaA"
                     /locus_tag="VIW27_01055"
     CDS             5864..6874
                     /gene="moaA"
                     /locus_tag="VIW27_01055"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5738276.1"
                     /translation="MSATSNSKQLVDRFGRTVSYVRLSVTDRCDLRCVYCMPEKMTFV
                     PRTQLLTLEEMESVGRAFIRLGVDKIRITGGEPLVRQNIMQLFRNLGACDGLRDLTLT
                     TNGTQLVKYAQPLREAGVTRINISLDTLDEDRFRSMTRVGDLQKTLDGIDAAIDAGFG
                     QIKLNTVVMKNHNHDEILDLVNFCIGRGIDISFIEEMPLGQNDHHDRAVAYYSSDQIL
                     EDLERHFELVPSTMTTAGPTRYYRLTGHDNTRVGFISPHSHNFCDSCNRVRVTAEGRL
                     LLCLGQEHSMDLRHVIRAHPGEPEVLEQALIDSMQIKPKGHDFNLNSQEVLLRHMNLT
                     GG"
     gene            6876..>7679
                     /locus_tag="VIW27_01060"
     CDS             6876..>7679
                     /locus_tag="VIW27_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY5738277.1"
                     /translation="MSSVPKRRLPELTDAGLKATANVVSVNEYNQPVEGAIAVERALT
                     IYLDKREIVTLMTLGNHPELLILGWLRNQRMIDDIEAVRAVQVDWEIESAAIVTRDGV
                     DDLDDKLERKTVTTGCGQGTVFGDLMDDIDEIRLTPPRLRQSAIYALLKALNEYNEVY
                     KNAGAVHGCALCSESQGIELFIEDVGRHNAVDAIAGHMWLHDIGGADKLFYTTGRLTS
                     EMVIKVALMGIPVLLSRSGITAMGLEIAQRVGVTLIARAKGRHFLVYHGG"
BASE COUNT         1395 a         2486 c         2389 g         1409 t
ORIGIN      
        1 cgaagaattg ttgctgaaat tcgtggcccg ctccgtcagc gatgataaca gaaaaaactt
       61 caaaaatcgc agaaatatga tcctccggct cgtgcgtatc ggggctgcgc tcgaatccca
      121 gcgcacgcag atcgtcacgt agttcgctga gcggctgctc catcaggaag ccggtcaggt
      181 accaggatcc gtagggcacc agttctccgc ggccgatgcc gataaacagc gcgtggtact
      241 cgtcgtcgat ctgttcgagg ctggatttcg cggcgctcgc cgacagttgc cgcatcgcct
      301 cgagcaattc gtcgggctcc gcgtcactgc ccggtgaaag tcgcgccacg tgctccagca
      361 atgcccgatc gggcggcgca cgcagcagcg cggccagcag gctgtaggcg gcagcccggt
      421 aaacctgttc gtgttcggtg gttgcgtctg cggctggcac gggtatcgat atctccaaaa
      481 ttactggtta ctggcgaaaa ccctttagcg ggtcgaactg tccacccatc gcctcggggt
      541 cctggacgat atcggcaacg cggcaattct cgcacatttt caggcgatcg agtgcgcgcc
      601 ggctgccgaa catgtaatgg tccctgagtt tgtcctgcat cttgttgatt accgaggtcg
      661 gtgcgaacgg cttgccgcag gcgatgcagg cgaacggagc atccgagttc agcgcccgcg
      721 cgcggttgcg ccgctcgcga tcgaacaaca gccgcggtcg cagcgtgatc gcgtcttcgg
      781 ggcaggtttg cacgcaggcg ccgcactgca ggcaattgcc ctcgacgaag agcacctcgg
      841 gcagctcgcg gttcgatccg tcctgcagcg ccctgcccgg gcaggcgccg acgcaggcca
      901 tgcacagcgt gcacttgccg gcgtcgatga gcgcctcgcc gaacggcgcg cccggtggca
      961 actcgacctc gccggacgcc ggtcgcagct ggcccgccag gtggtcgagc gcctgataaa
     1021 tagcgctgcg cttgagcagc ggcatatcga gcacggacgg ctcgaggtcc ggactcgaat
     1081 cgagcgtcgg caacggcgca tcggccgcca gcaggcaggc atgggccgaa cccatgccca
     1141 gcggggcgat cagagcgtgc agccagtcga gctgggcgcg cagattgtcg cgcgtgctcg
     1201 ccggaactgc atcgtcgacc agcagcagca cctgcgaggc gccgtaagca aacgccgaca
     1261 ggcacaggtc catgccgatg ctgccgagtt cctcgacggc gaccggcaac aggtcgtcgt
     1321 aggcctcgag ataggcgtcc ggcgcgcaag tttcggcatg gaacatgacg atcggctggc
     1381 ggccgccgtg ctcgcggaac gcggccagca tgtcccgcag gcgcttgccg ttgtcgcgca
     1441 ggttcgggta gcggtaacga atcgcgcccg acgggcaaac cgtcgcgcaa ctgccgccgc
     1501 cctggcacag gttcgggttg acctcgatgc gctcgccgat gctgacgatc gcttctgccg
     1561 ggcaggcgtc gatacagcga ctgcagaccg tttggccgtt gaccgagtgg gcgcagatcg
     1621 aggcgtcata gtcgaagtac ctgggttttt cgaactcgcc ggacagattc tcgagttcgg
     1681 ccaccgtatc cagactgtcc cactcctccg gcggcgcatg gaggtaacca ggcggcggca
     1741 tttcgcgagc cagcagcggc cgcgaattga gatcgagcac cgcatcggcg cgatgcgcag
     1801 ccgtgttgcc cgcggcgtcg gtgacaccga cggtgaaagc gccgagatag ccatcgatcg
     1861 agacgctgtc ggcgcccgcg gcaacacgct cgatatcgag ccccggcggc aatcgatcgc
     1921 agtgctcgag tgcggcgtcg tcaccgacgg cgaggatgcg ccccgccgac ccgtagctaa
     1981 ccaggtcggt cgcttccagc ggtgcgcggt cgcgcgcctc gagagcatgc tgacgagcgt
     2041 cgctattact catctcgcgt cccctgcgcc ggtctcgccg gcggtctcca gacgcgccat
     2101 ctggtcggcg tccggttcgg cgtcgtcccg cgcctcgtct tcggcgccgt cgatatcctc
     2161 gacggcgtcg agttcggcgc tctcggattc gaccgcatcg tcggccgcct gttcctcggc
     2221 cagctggtcg cgcagtttct gctgctccat ctcgatgcgg tgcttcatgt cactggtgac
     2281 gatatcgccg agcttctcga agctggtgaa atcatcgtcg tattcgtcga ggccatcgcg
     2341 aatattgaag gccggcgcat gaaacagctt gcgcagcgcg aggttgcgga gttcgtcgct
     2401 gaccccggcg gacatgaact ggctgaagtc ggaattctca tccagcgagt cgatagccgg
     2461 catgtcggca tcggtgagca gcggcttctc cgcctcggcc gcccgctcca cggcttgctc
     2521 ggctggcgcc ggcctgtctt ccgtcatggc tgcggacgat ggccggcggg gttcggcctg
     2581 ctgctggcgg gcctcgagct tgcgccgcga ccagcgcgac agcgccgatt cgtcgccgcg
     2641 cccgctctgc ttgctgtcgg cggtcacgac tgctcgcctc gcggcggccc atcggctgcc
     2701 tgcgggtcgc gccagttcct gagcttgcgc ttggtcttct tgaccgccac gtaatgcgtc
     2761 agcacataag cctcggccca gcgatagagt tccggcggaa tcggcaccgc gtagacttcc
     2821 tcgtcgccct cgccataggc gtgcacctcg tcgaaactca gcgaaaccag gtagggctcc
     2881 ggcatttcgg catcgctgtc ggggcggttg gcgaccacga aacagcccgg ctcgggcgac
     2941 atcaggttgt gatagtagct ttcgcactcg tcttcgtaga gctggactct gagtccggtc
     3001 accaggaaat gctcgatatc gccgtcaacg aagacgcgtt ccacccggta ctcgtcgtcc
     3061 ggcccgcgaa ccaccacgcc gagcgcctgg taacggaagt ccgcccaggg atgcgtcgac
     3121 ggctgctttt ccatcatcac cgcaacgttc atatggcccg gcaattcctg gtcgaaatcg
     3181 tcgagttcga gttttgccat ggatctccaa cggtaccgcg attcagggat ttgcagttat
     3241 acacagcttg ggcattgtcc cgcaacctca cttttttcag ggataaacgc ctggcccgcg
     3301 cagatcgggt tcccaaattt ggctattcgg gagattgata gcttcggcaa gcccgctaga
     3361 acgggcttgc atcgggagca tccgtggatc gagatcgcta ttgaggggat cagagtaaaa
     3421 actccccgat caacgcaatt tcgagcaacg tttgacgaaa tctttgccca agcaaagatt
     3481 tcgccgactc gcgcagccgt cagtaaaggc tttatcggaa tttttgctcc gaccccgatt
     3541 gttcgatgcc cggaagcgct gcgtcgaatg gagctgcaat tttggcaaga ttgaggactg
     3601 accagctgtc agcgggcttc acctgttgtc ttcgccgcta ccgacagctg ctctgccccc
     3661 tcgaagagag caaagcatgt cgagcctcct cagttgtcgg ccgaaggtcg ctcaaagcgg
     3721 atccttgcaa ctttttggca ggtccgacga gaccggatta ttgcctgcaa gccgaggaca
     3781 cagctatcta agttacttcg agggtgaaat cacgctcgtt gtcgagcacc ggaatgcgct
     3841 gcctgacttt ggcaactttt cccagatcga tgtcggcgaa caccacgccc ggttcggcac
     3901 cgccgtcggc gagcacctcg ccccacgggt cgacgatcag cgagtggcca taggaatagc
     3961 gcttgtcggc atgatgcccg cactggttgg gggcgagcac gtaagccccg gtttcgatcg
     4021 cccgggcgcg caccagcacg tgccagtgcg cctcgccggt agtctgcgtg aatgccgccg
     4081 gaatggtaat gatctgcgcg ccgccatgcg ccagcgcgcg gtaaagtgcg ccgaagcgca
     4141 ggtcgtaaca gaccgacagg ccgatgcgac cgagcggcgt gtcgaccacc accgcgcgcc
     4201 cgccgggggc aatcgcatcc gattcgcggt acgcctcgcc gttgcgcagt tcgatatcga
     4261 acatgtgcag cttgtcgtag cgcgcacgaa tttcgccctg ctcatcgagc aggaaactgc
     4321 ggttgtgaat cctgtcgtcg tcgcgaatca gttgcgagcc gaccagtatc catacgccgt
     4381 gctggcgcgc ccgcccgcgg aacgccgcca gtccgatatc gtccacctcg ccgtaggtgg
     4441 ccggcggaat ccgctcgttg accggctcga tcaggcccac ggtttccggc agcgcgacga
     4501 aatcggcacc gcctgcgacc gcctcgtcta tttgcgcgca gacgcgctcg acgttggcat
     4561 gaatatcgcg gcccgcggtg ttctgcacgc aggcgacacg aatctcagtc atgcctcagc
     4621 acttcctcgg cggcaaagta atcgtagccg agatcgtgcg cgacctcgcg ctgcgtgacc
     4681 ttgcccttgt gcacgttgag gccgtcgcgc agccactggt cttccatcag cgcacgccgg
     4741 taacccttgt cggcgagcgc cagcacgtgc ggcagggtca cgttgttgag cgcgtaagtc
     4801 gaggtgcgcg gcaccccgcc cggcatgttg gcgacgcagt aatgcaccac gtcgtcgacg
     4861 atataggtcg gatcggcatg ggtagtcggt ttcgaggttt cgcagcaacc accctgatcg
     4921 atggcgacat cgacgatcac cgcgcccggc ttcatgttgc gcaccatctc ggcagtcacc
     4981 agcttcggcg cctcggcgcc cgggctcagc acaccgccga tcaccaggtc ggcgtcgatc
     5041 acgtgacgtt cgatcgcgtc ctcggtcgag aacacggtat tggtgctgcg gccgaattgc
     5101 ttccagtgcg cctcgagaac ttcggcattg cggtcgatga cccagatgtc ggcgcccatg
     5161 ccgactgcga tatgcgtggc atgcgtgccg accacgccag cgccaagaat gacgaccttg
     5221 gccgggtcga cgccgggcac gccgccgagc agcatgccga gaccgccgtg cgggtgctcg
     5281 aggcaatagg cgcctgcctg gatcgccatg cgcccggcga ccttggacat cggcgccagc
     5341 agcggcaggc cgccatgcgg cgaggtcacg gtttcgtagg cgatacaggt cgcgccgctg
     5401 tccaccagtt cgcgggtctg ctcgggatcg ggcgccaggt gaaggtaggt gaacagcacc
     5461 tgcccttcgc gcagcatcgc acgctcgacc gcctgcggtt ccttgacctt gaccaccagc
     5521 tcggcctcgg caaatatttc ctcgggcgta tcgacgatgt gcgcgccgac ctgccggtag
     5581 tcatcgtcgc tgacgccgat accgtctccg gccagggttt cgaccatgag ctcgtgcccg
     5641 tgcttgattg cttcgcgcac gctggccggg gccataccga cgcggtattc gtgattcttg
     5701 atctccttgg gaacgccgat gagcataata accgcctcga aagtagggaa agcccgcagt
     5761 ttaaccagcg ccccgcggcg tgctagtgcc agcgatattt atttactgta ccagtaatga
     5821 cgcgttccaa ttcccggctt tcttgcatag aataggtccc cttatgtccg ccacaagcaa
     5881 cagcaagcag ctcgtcgacc gcttcggccg cacggtcagc tatgtgcgcc tctcggtgac
     5941 cgaccgctgc gatctgcgct gcgtctactg catgccggaa aagatgacat tcgtaccgcg
     6001 cacgcagctg ttgaccctcg aggagatgga aagcgtcggc cgcgcgttca tcaggctcgg
     6061 cgtcgacaag attcgcatca ccggcggcga accgctggtg cggcagaaca tcatgcagct
     6121 gttccgcaac ctgggcgcgt gcgacggcct gcgcgacctg acgctgacca ccaacggcac
     6181 gcagctggtc aaatacgcac agccgctcag ggaagccggc gtcacgcgca tcaatatcag
     6241 cctcgatacg ctcgacgagg atcgctttcg cagcatgacg cgcgtcggcg atctgcaaaa
     6301 gacgctcgac ggcatcgacg ccgcgatcga cgccggcttt gggcagatca agctcaatac
     6361 cgtggtgatg aaaaaccaca accacgacga aattctcgac ctcgtgaatt tctgcatcgg
     6421 tcgtggcatc gatatcagct tcatcgagga aatgccgctg ggccagaacg accaccacga
     6481 ccgagcggtg gcctattact ccagcgacca gatcctcgaa gacctcgagc ggcacttcga
     6541 gctggtgccg agcacgatga cgaccgcggg gcccacgcgc tactaccggc tgaccgggca
     6601 cgacaatacc cgcgtcggct tcatttcgcc gcacagccac aatttctgcg acagttgtaa
     6661 ccgcgtgcgc gtcaccgccg agggccgcct gctgctgtgc ctcgggcagg aacactcgat
     6721 ggacctgcgc cacgtgattc gcgcgcatcc cggcgaaccc gaggtgctcg aacaggcact
     6781 gatcgattcg atgcaaatca agccgaaagg gcacgatttc aacctcaact cccaggaagt
     6841 cctgctgcgg cacatgaatc tgaccggggg atgagatgtc ctcggtaccg aagcggcgtt
     6901 taccggagct gaccgacgcc ggtctcaagg cgaccgccaa cgtggtttcc gtcaacgaat
     6961 acaaccagcc cgtcgagggc gcaatcgcgg tcgagcgcgc actgacgatc tacctcgaca
     7021 aacgtgaaat cgtgaccctg atgacgctcg gcaatcaccc cgagctgctg atcctcggct
     7081 ggctgcgcaa ccagcgcatg atcgacgaca tcgaggccgt cagggcagtg caggtcgact
     7141 gggagatcga gtcggcggcg atcgtcaccc gcgacggcgt cgacgatctc gacgacaagc
     7201 tcgagcgcaa gaccgtgacc accggctgtg ggcagggcac tgtgttcggc gacctgatgg
     7261 acgatatcga cgagatcagg ctgactccgc cgcggctcag gcaatcggcg atctacgcac
     7321 tgttgaaggc gctcaacgag tacaacgaag tctacaagaa cgccggcgcc gtgcacggtt
     7381 gcgcgctgtg cagcgagtcg cagggtatcg agctgttcat cgaggacgtc ggccgccaca
     7441 acgccgtcga cgcgatcgcc ggccatatgt ggctgcacga catcggcggt gccgacaagc
     7501 tattctacac caccggccga ctgacctcgg agatggtcat caaggtggcg ctgatgggca
     7561 ttccggtact gctgtcgcgc tccggcatta ccgcgatggg gctggagatc gcgcaacgcg
     7621 tcggcgtcac gctgatcgcg cgcgccaagg ggcggcattt cctggtctat catggcggc
//
LOCUS       DATCZV010000045         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_426913, whole genome shotgun sequence.
ACCESSION   DATCZV010000045 DATCZV010000000
VERSION     DATCZV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426913"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VIW27_01065"
     CDS             complement(<1..351)
                     /locus_tag="VIW27_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY5738278.1"
                     /translation="MIHRGVLAHWLVLLCLACSFSAAAQTPPSARDVATYPPLFLAVV
                     NADETMLQRAIASAEDIDARDDHGRSALHVAAFYANHAAMRALIAAGADANALENDYY
                     DIATIAAVADDVDTL"
     gene            complement(355..1599)
                     /locus_tag="VIW27_01070"
     CDS             complement(355..1599)
                     /locus_tag="VIW27_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5738279.1"
                     /translation="MKLDIDFVRAQFTQLGDDSESAFVFASNAGGSYVCNPVNEILEH
                     YNRHTRVQPYSRYPSSTAAGVAMDRARRVWSEALNIAPDELTFGPSTSMNTYVMAQAI
                     GDAWQPGDEIVVTNQDHEANSGVWRRKALEKGVIVRQWEVDPQTGLLDLNHLYPLLGE
                     HTRWVFFSHCSNVVGTANPVSDIVEAVKTGSPARVFVDAVAYAPHHLCDLKALGVDGY
                     AFSLYKVYGPHQGLLYVNAEIRGDLTSQAHYFNAGLPQKDFNPAGPQHAQVAACAGVI
                     DYFDALHEHHFGAEEASANQRLQALHELILAHENSLAAPLLEFLDGHAEVRLVGKPHV
                     DDNDRAPTIAFRPLAHSAADVAHKLQDAGIGTEHGNFYAHRLVRDLGIEPDDGVVRLS
                     LVHYTHPDEVERILQALHAALG"
     gene            complement(1621..2505)
                     /locus_tag="VIW27_01075"
     CDS             complement(1621..2505)
                     /locus_tag="VIW27_01075"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEY5738280.1"
                     /translation="MPESLEQRIAGLPCWNGEIQIEPLGGGMTNLNFKVVDGERACVV
                     RFGVDDPVHLISRANEIAAGDAAHALGVSPQPLYREDGIQVLSFVDGQVYGEADVRVD
                     ANLERIVELLQRFHREMPQHFNGVPVMFWVFQVLRHYCNLLERGASPHAGRLPELARI
                     ARELETAVGPVEIVYGHNDLLAANFIDDGEKIWLIDFDYAGFNSPLFDLANLASNNEL
                     DPAQETAMLEQYYGAPPDRARRTSFEAMKCASLLRETLWSMVSELHSRVDMDFADYTQ
                     KNLERFESALSAYRREFT"
     gene            2556..3353
                     /locus_tag="VIW27_01080"
     CDS             2556..3353
                     /locus_tag="VIW27_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738281.1"
                     /translation="MRNLARQFFALGWCRFAPDPLLGDWVDAALPAARASVTDSRHAR
                     WLRYQGTWFAGVNALPNDARGALDGSGPLAGAAVDFIAAELGLADFAWDPAQVSICYP
                     GYPQPMDGESEALLRYRRDRDAAHVDGLLREGAERRRFLREFHGFILGIPMVEFDADA
                     SPFVIWEGSHELVRAAFRQRFADIPPGRWSDEDVTEAYQAVRQRVFETCPRAAVHARP
                     GEAFLVHRLSVHGMAPWRDGAKAGPDGRMIVYFRPDTFGPFEWLNNP"
     gene            complement(3493..>4138)
                     /locus_tag="VIW27_01085"
     CDS             complement(3493..>4138)
                     /locus_tag="VIW27_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5738282.1"
                     /translation="MNRLDHIVVAATTLEQGAEYLRGLLGIEVPVGGVHATMGTHNRL
                     MQLGDDSYLELIAIDPAGTVPARPRWFGLDTALLRAAIAQRPRLITWVMNTPDLAAVA
                     AAGDFAIGTPTALSRDGLSWEIALTDDGRLLADGLLPYCIQWHSSPHPSRAMADLGCR
                     LKKLTLHHSRPDWLAARLATLGATDLVEIDEIPDSESPYLSAELETPNGLVRLT"
BASE COUNT          671 a         1275 c         1422 g          770 t
ORIGIN      
        1 cagggtgtcg acgtcgtcgg cgaccgcggc gatggtggca atatcatagt aatcgttttc
       61 cagtgcattg gcgtcggcgc cggccgcgat cagcgcgcgc atcgccgcgt gattggcgta
      121 aaacgccgcg acatgcagcg cgctgcggcc gtgatcgtcg cgggcgtcga tatcctcggc
      181 gctggcgatg gcgcgttgca gcatggtctc gtcggcgttg acaacggcga ggaacagcgg
      241 cgggtaggtt gccacgtctc gcgccgaggg cggtgtctgc gcggcggcag aaaagctgca
      301 ggccaggcac agcaaaacca gccagtgtgc cagcactccg cggtgaatca tggttcagcc
      361 cagcgcggcg tgcagcgcct gcagaatgcg ctcgacttcg tcggggtgcg tgtagtgcac
      421 cagcgacagg cgtaccacgc cgtcgtcggg ctcgatgccg agatccctga ccaggcggtg
      481 ggcgtaaaaa ttgccgtgct cggtgccgat gcccgcatcc tgcagcttgt gggccacgtc
      541 cgcggcgctg tgcgcgagcg ggcgaaacgc gatcgtcggt gcccggtcgt tatcgtcgac
      601 atgcggcttg ccgaccaggc gtacctcggc atggccgtcg aggaactcga gcaacggcgc
      661 ggcgagcgaa ttttcatgcg cgaggatcag ttcgtgcaat gcctgcaacc gctggttggc
      721 gctggcttct tcggcgccga aatggtgttc gtgcagcgcg tcgaagtaat cgatcacgcc
      781 ggcgcaggct gcgacctgcg cgtgttgtgg cccggcggga ttaaaatcct tttgcggcag
      841 gccggcattg aagtagtgcg cctgtgaggt caggtcaccg cgaatctccg cgttgacgta
      901 cagcagtccc tggtgcgggc cgtagacctt gtagaggctg aacgcgtagc cgtcgacgcc
      961 gagcgccttc agatcacaga gatggtgcgg cgcgtaggcg accgcgtcga cgaatacccg
     1021 cgccgggctg ccggtcttca ccgcctcgac gatgtcgctc accgggttgg cggtgccgac
     1081 gacattcgag caatggctga aaaataccca gcgcgtgtgc tcgccgagca gcgggtagag
     1141 gtgattcaga tcgagcagtc cggtttgcgg atcgacttcc cactggcgaa cgatgacgcc
     1201 tttctccagc gccttgcgcc gccacacgcc gctgttggcc tcgtgatcct ggttggtgac
     1261 aacgatttcg tcgcccggct gccaggcgtc gccaatcgcc tgtgccatga cataggtgtt
     1321 catcgacgtc gacggcccga aggtcagctc gtcgggcgcg atgttcaacg cctccgacca
     1381 gacgcggcgg gcgcggtcca tggcaacgcc ggccgcggtc gacgacgggt agcgcgaata
     1441 gggctgtacg cgggtatggc ggttgtagtg ctcgaggatc tcgttgaccg gattgcagac
     1501 atagctgccg ccggcgttgg acgcgaacac gaacgcggat tcggaatcgt cgccgagctg
     1561 cgtgaactgc gccctgacga agtcgatgtc gagtttcatt gagttcattc tccgtgggtg
     1621 ctaggtgaat tcgcggcggt aggcggaaag cgccgactcg aaccgctcga ggtttttctg
     1681 cgtgtagtcg gcgaaatcca tgtcgacgcg cgagtgcagt tccgagacca tcgaccacag
     1741 ggtctcgcgc agcagcgacg cgcatttcat cgcctcgaag gacgtacgcc gggccctgtc
     1801 gggcggtgcg ccgtaatact gttcgagcat ggccgtttcc tgcgccgggt cgagttcgtt
     1861 gttggaggcg aggttggcga gatcgaacag cgggctgtta aagccggcgt aatcgaaatc
     1921 gatcagccag attttctcgc cgtcgtcgat gaaattcgcc gccagcaggt cgttatggcc
     1981 gtagacgatt tccaccgggc cgaccgcggt ctcgagttcg cgggcgatgc gcgcgagttc
     2041 gggcaggcgc ccggcatgcg ggctcgcgcc gcgttcgagc aggttgcaat agtgccgcag
     2101 cacctggaat acccagaaca tcaccggcac gccgttgaag tgctgcggca tctcgcggtg
     2161 aaaacgttgc agcaactcga cgatgcgctc gaggttggcg tcgacgcgca catcggcttc
     2221 gccgtagacc tggccgtcga cgaagctcag gacctggatt ccgtcctcgc ggtacagcgg
     2281 ctgcggactg acgccgagcg cgtgcgcggc gtcgccggca gcgatttcgt tggcgcgcga
     2341 gatcaggtgt accgggtcgt cgacgccgaa acgcacgacg caggcgcgtt caccgtcgac
     2401 gaccttgaaa ttgagattgg tcatgccccc gccgagcggt tcgatttgaa tttcaccgtt
     2461 ccagcagggc agtccggcga tgcgttgttc gagcgattct ggcaaggttc cgtggctccg
     2521 gatctggatc gaggcatgat agcatcggcg gcatgatgcg caatctcgct cggcaattct
     2581 ttgctctcgg ctggtgtcgc ttcgcgccgg acccgctgct tggcgactgg gtcgacgcgg
     2641 cgctgccggc ggcgcgggcc agcgtgacgg attcgcgtca tgccagatgg ctgcgctacc
     2701 agggcacctg gttcgccggc gtcaacgcgc tgcccaacga cgcgcgcggc gcactcgacg
     2761 gcagcggtcc gctggccggc gctgcggtcg acttcatcgc cgccgaactc gggctcgcgg
     2821 acttcgcctg ggacccggcc caggtgtcga tctgttaccc cggctatccg cagccgatgg
     2881 acggcgagag cgaggcgttg ctgcgctacc gccgcgaccg cgacgccgcc catgtcgacg
     2941 gcctgctgcg cgagggagcc gagcggcggc gcttcctgcg cgaattccac ggcttcattc
     3001 tcggcatacc gatggtcgag ttcgatgcgg acgcgtcgcc gttcgtcatc tgggagggct
     3061 cgcacgaact cgtgcgcgcc gcgtttcggc aacgcttcgc cgacattcct cccggacgct
     3121 ggagcgacga agacgtcacc gaggcttacc aggcggtgcg ccagcgcgtc ttcgagactt
     3181 gcccgcgcgc cgcggtgcat gccaggccgg gcgaggcctt cctcgtccat cgcctgtcgg
     3241 tacacggcat ggcgccgtgg cgggacggcg cgaaggccgg ccccgacggc cgcatgatcg
     3301 tctactttcg ccccgacacc ttcggcccct tcgaatggct caacaatccc taaaatgggg
     3361 tcagagtaaa atttctacta aatgcgttta aattcgggtc agagtctttt ttgggctttg
     3421 aggacgttaa ttcgactctg accccaattt attcgatccc tgaaaggtgg agtccctgcc
     3481 tggaagccgg tatcaggtca gccgaacgag gccgttcggg gtttccagtt cggccgacag
     3541 gtaagggctt tccgagtcgg ggatttcgtc gatttcgacg agatcggtcg cgccgagggt
     3601 tgcgagacgc gcggcgagcc agtcggggcg actgtgatgc agcgtcagct ttttcagccg
     3661 gcagccgagg tcggccatcg cgcgcgacgg gtgcggactg ctgtgccatt gaatgcagta
     3721 cggcagcagg ccgtcggcga gcaggcggcc gtcgtcggtc agggcgattt cccaggacag
     3781 tccgtcgcgg ctgagtgcgg tcggcgtgcc gatggcgaaa tcgccagccg ctgcgactgc
     3841 cgccagatcg ggcgtgttca tgacccaggt gatcagtcgc gggcgttgcg cgatcgccgc
     3901 gcgcagcagc gccgtgtcga ggccgaacca gcgtggccgc gccggcaccg tgccggccgg
     3961 gtcgatggcg atgagctcga gatagctgtc gtcgccgagc tgcatcagcc gattgtgcgt
     4021 gcccatggtc gcgtgcacgc cacccaccgg cacctcgata ccaagcagac cacggaggta
     4081 ttcggcaccc tgttcgaggg tggtcgcggc gacgacgatg tgatccagcc ggttcatg
//
LOCUS       DATCZV010000046         5161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_109241, whole genome shotgun sequence.
ACCESSION   DATCZV010000046 DATCZV010000000
VERSION     DATCZV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109241"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VIW27_01090"
     CDS             complement(<1..369)
                     /locus_tag="VIW27_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5738283.1"
                     /translation="MPLLQASNLHHGYGDRPLLDGVDFILEAGERVCLVGRNGSGKST
                     LLAILGGEMRADEGQVRHPSELRIASLPQEVPGELPGSVYDCVAAGIGKLAGLITDWH
                     HASLAASRDPQALDTMQRLQD"
     gene            442..1092
                     /locus_tag="VIW27_01095"
     CDS             442..1092
                     /locus_tag="VIW27_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY5738284.1"
                     /translation="MCGRFFLDAQAGEIIEHYNAPPPDLFTARYNIAPTTPVLAFGGN
                     KFDLYRWGLIPSWAKDISVGNRMFNARAETVAEKPSFRNAYRRRRCLLPANGFYEWRL
                     EQGRKQPYVCHLGRRLFSMAGIWERWQDAGGNEVNSCAVITTDAKGAMRELHQRMPVC
                     IAPADYAVWLDCSSDRTDAADRLLRNNAPAYEFYPVGLAVGNSRNEGSALIEPLSH"
     gene            1105..2694
                     /locus_tag="VIW27_01100"
     CDS             1105..2694
                     /locus_tag="VIW27_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY5738285.1"
                     /translation="MRTPSHRTIAASWIRPGSRPIAVLALLAWLLSGCVSLPNNDDRV
                     ASYAFTDTGDTLLGRGFADARQQHAGKDGFLLLGNGLDAFTARAALAEAAERSLDVQY
                     YLYHDDMVGGLFTAFLWRAAERGVRVRLLVDDMDLAGRDRGVVALDAHPNIEIRIFNP
                     FDRSFSRLPQFVTGLGSVTRRMHNKSFIVDNVAAILGGRNIGDEYFDADPALAFADLD
                     VIAVGDVVGQVSNAFDRYWNSASVYAARKIITEEISDDQIRQIQQRLLTFIDENRDSE
                     YMQALRESDLAQKIRDRSLSFFWGNADLVVDDPDKITSSRDASELHLATQLAPHFDAL
                     RNELVIVSPYFVPGTEGVEFFSRLLERGVRVRILTNSLASNDVAAVHAGYARYRKPLL
                     RAGVELYELNKTVSRVQHGGKKGAGGSSKASLHAKTFVLDRREVFIGSLNLDPRSFYE
                     NTEIGLVIEQGEIAAGMAAGLEREIERVAFRLELGEDGNLLWHGYEDGAAVTFDVEPH
                     TGFWQRFGVGLLRLLPIESQL"
     gene            complement(2739..3035)
                     /locus_tag="VIW27_01105"
     CDS             complement(2739..3035)
                     /locus_tag="VIW27_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY5738286.1"
                     /translation="MAKLTTVYWRDIPAQVIAKERRDTAKAVLGERFAEAIDRAAMRA
                     KMAGTDAYLEQWRREVVNCGGDLQAAVDTAAAELEQAFDDARLQALVENGGLDS"
     gene            3203..4162
                     /locus_tag="VIW27_01110"
     CDS             3203..4162
                     /locus_tag="VIW27_01110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5738287.1"
                     /translation="MLRVGFIGLGNVGGKLAGSLLRNGVDLTVRDLDRDVAQPFLDRG
                     AKWADSPREMAESVDVVITCLPSPAASAAVLESPDGVLAGLGANKIWAEMSTTDRDEV
                     VRLGAKVRATGAEAVDCPVSGGCHRAATGNISIFAGCERATFERILPLLTTMGRRILH
                     TGELGSASVLKVVTNYLATANLVSLCEALVASKMAGMDLNTAYEAIRISSGNSFVHET
                     ESQVILNGSRDISFTMDLVCKDVGLFNAVAERAGLQLELAPLLLRIFEEGQQRYGARE
                     FSDNIIKRLEEQAQVEILAPGFPPEMVDDEPEEPGYEVKVRGR"
     gene            4415..>5161
                     /locus_tag="VIW27_01115"
     CDS             4415..>5161
                     /locus_tag="VIW27_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5738288.1"
                     /translation="MMAVVTTGNGGYDKLDYREVPVPEPGPGEVLLRVLAAGVNNTEI
                     NTRLGWYSSSVISGTEQYAGSEPAADNLADGGWNQATPFPFIQGTDCCGEVVALGDGV
                     VGEFAGKRVLVRACMRLHGFDSLENIWMASDFDGAFAQFVRVPASEVFTVDCNWSHAE
                     LATIPCAYGTAENMVHRAGVARGERVLVTGASGGVGSAVVQLARRRGAQVIAVAGKAK
                     VEAVAVLGPERVIERGCSLLGELGPRSVDVV"
BASE COUNT          877 a         1621 c         1684 g          979 t
ORIGIN      
        1 gtcctgcagg cgttgcatgg tgtccagcgc ttgcggatcg cggctcgcgg ccagcgacgc
       61 gtggtgccag tcggtgatca ggcccgcgag tttaccgatc ccggcggcga cgcagtcgta
      121 gacgctgccg ggcagttcgc cggggacttc ctgcggcagc gatgcgatgc gcagttccga
      181 tggatggcga acctggccct cgtcggcgcg catctcgccg ccgaggatcg ccagcagggt
      241 cgacttgccg ctgccgttgc gtcccaccag gcacacgcgc tcgccggctt cgagaatgaa
      301 atcgacgcca tcgagcaacg gccggtcgcc gtagccgtga tggaggttgc tggcctggag
      361 caggggcatg ggtgtcgccg tggttaaact gcgttattat gcggctttga tacaaataat
      421 gcagtcgccg cgggaggatt catgtgcggg cgattttttc tcgacgcgca ggccggagag
      481 atcatcgaac actacaacgc gccgccgccc gatctgttta cggcgcgcta caacatcgcg
      541 ccgaccacgc cggtgctcgc cttcggcggc aacaagttcg atctctatcg ctggggcctg
      601 atcccgtcgt gggcgaagga catcagcgtc ggcaaccgca tgttcaacgc gcgcgccgaa
      661 accgtcgccg agaaaccgtc gtttcgcaat gcctaccggc gacggcgctg tctgctgccc
      721 gccaacgggt tctacgaatg gcggctcgag caaggccgca agcaacccta cgtctgccat
      781 ctcggtcggc gcctgttttc gatggccggt atctgggaac gctggcagga cgccggcggt
      841 aacgaagtta attcctgcgc ggtgatcacc accgatgcga aaggcgcgat gcgcgagctg
      901 caccagcgca tgccggtctg tatcgcgcct gctgattacg cggtatggct cgattgcagc
      961 agtgacagaa ccgatgccgc cgaccgcctg ttgcggaata acgcgcccgc atacgagttt
     1021 tacccggtcg gcctcgcggt gggcaattcg cgcaacgagg gctcggcgtt gatcgagccg
     1081 ctcagccact aggtcggagt cgagatgcga acccccagtc accgaaccat cgctgccagc
     1141 tggatccggc cgggtagccg tccgatcgcg gtactggcgt tgttagcctg gctgctgtcc
     1201 ggctgcgtgt cgctgccgaa caacgacgat cgcgtcgctt cctacgcctt caccgatacc
     1261 ggcgatacgc tgctcggccg cggcttcgcc gacgcccggc agcagcatgc cggcaaggac
     1321 ggctttctgc tgctcggcaa cgggctggac gcgttcacgg cccgcgccgc gctcgccgag
     1381 gccgccgagc gcagcctcga cgttcaatac tacctgtatc acgacgacat ggtcggcggc
     1441 ctgttcaccg ctttcctgtg gcgggccgcc gagcgtggcg tgcgggtgcg cttgctggtc
     1501 gatgacatgg acctcgccgg gcgcgaccgc ggcgtcgttg cgctcgatgc ccatccgaac
     1561 atcgagattc gcatcttcaa tccgtttgat cgcagtttca gccgcctgcc gcagttcgtc
     1621 accggcctgg gatcggttac tcgccgcatg cataacaagt ctttcatcgt cgacaacgtc
     1681 gccgccattc tcggcggacg taatatcggc gacgagtatt tcgatgccga tccggcgctc
     1741 gcgtttgccg acctcgacgt tatcgcggtc ggcgatgtcg tcgggcaggt atcgaacgcg
     1801 ttcgaccgtt actggaattc ggccagcgtc tatgcggcca ggaagatcat caccgaggag
     1861 atcagcgacg accagatccg ccaaattcag cagcgcctgc tgaccttcat cgacgaaaac
     1921 cgcgattcgg aatacatgca ggcgttgcgc gagtccgacc tcgcgcagaa aatccgcgac
     1981 cggagcctgt ccttcttctg gggcaacgcc gatctggtag tcgacgatcc ggacaagatc
     2041 accagctcgc gggacgccag cgaattgcat ctggccacgc agctcgcacc ccacttcgac
     2101 gcactgcgca acgaactggt gatcgtttcg ccttacttcg ttcccggaac cgaaggcgtc
     2161 gagttcttct cgcggctgct cgagcgcggt gtcagggtaa gaattctcac caactcgctg
     2221 gcttccaacg atgtcgccgc ggtacacgcg ggttacgcca ggtaccgcaa gccgttgctg
     2281 cgcgcgggcg tcgaactcta cgaactcaac aagaccgtaa gccgcgtgca gcacggagga
     2341 aagaagggcg ccggcggttc gtccaaggcc agtctgcatg ccaagacctt cgtgctcgac
     2401 cggcgggaag tattcatcgg ctcgctcaac ctcgacccgc ggtcattcta cgagaatacc
     2461 gaaatcggcc tggtcatcga acagggggaa atcgctgccg gcatggccgc ggggctggag
     2521 cgggaaatcg aacgggttgc ctttcggctg gaactcggag aagacggcaa cttgctctgg
     2581 cacggttacg aggacggcgc agcggtcacc ttcgacgtcg aaccacatac tggcttttgg
     2641 cagcgtttcg gcgttggcct gttacgcctg ctgccgatcg aatcgcagct ttgatgcgcc
     2701 gcccgggcgg ctcgatggcc gcttgcccgg gtacccgctc aggaatccaa tccgccgttt
     2761 tcgaccagcg cctgcagacg ggcatcgtcg aatgcctgct cgagttccgc ggccgcggta
     2821 tcgacggctg cctgcagatc gccgccgcag ttgaccactt cgcggcgcca ttgctcgaga
     2881 taggcgtctg tgccggccat tttcgcgcgc atcgcggcgc ggtcgatcgc ctcggcgaaa
     2941 cgctctccga gcacggcctt ggccgtgtcg cggcgttcct tggcgatcac ctgggcgggt
     3001 atatcgcgcc agtagacggt ggtgagcttg gccatccggg taacctccga gcggatttta
     3061 tcagcgctaa cggggcgcga gtataagttc gccggcacgc cgaatccagc atgtttttct
     3121 ttgactggtc ggctgcgaaa acttgattgc tgagggccga tatcgggcgt agaatgccgc
     3181 gagttttcgt ggggggacaa ccatgctcag agtcggtttc atcggtctcg gtaatgtcgg
     3241 cggcaagctc gccggcagcc tgttgcgcaa tggcgtcgat ctgacggtca gggacctcga
     3301 tcgtgatgtc gcgcagcctt ttctcgatcg cggcgccaag tgggcggata gtccgcgcga
     3361 aatggccgag tcggtcgacg tcgtcatcac ctgcctgccg agcccggcgg ccagcgccgc
     3421 ggtgctggaa tccccggacg gcgtgcttgc cggtctcggt gcgaacaaga tctgggccga
     3481 gatgagtact accgataggg acgaggtggt tcgcctcggg gcgaaggtgc gcgctaccgg
     3541 cgccgaagcc gtcgattgcc cggtatcggg tggctgtcat cgcgccgcca ccggcaatat
     3601 ctcgattttc gccggttgtg agcgcgccac cttcgagcgc atactgccgc tgctgacgac
     3661 catgggccgg cgtatcctgc ataccggtga actgggatcg gcatcggtgc tcaaggtggt
     3721 caccaactac ctcgccaccg ccaacctggt gagcctgtgc gaggcgctgg tcgcctcgaa
     3781 aatggccggc atggacctga acacggccta cgaggcgatt cgcatctcgt ccggcaattc
     3841 ctttgtgcac gaaaccgaga gccaggtgat tctcaacggc agtcgcgata tcagtttcac
     3901 gatggacctg gtttgcaagg atgtcggcct gttcaacgcg gtagcggagc gtgccggcct
     3961 gcagctcgag ctggcaccgc tgttgctgcg gatcttcgaa gagggccagc agcgctacgg
     4021 agcgcgcgag ttttcggaca acatcatcaa gcgcctcgag gaacaggccc aggtcgaaat
     4081 cctcgcgccc ggctttccgc cggagatggt cgatgacgag ccggaagaac ccggttacga
     4141 ggtaaaggtt cgcggtcgct agattccggc gggccggggt ctaatcacca attggcgcaa
     4201 tctgccgcgc tccgccgagg agcactgagc cggggtgggg gctgcgccgt cgttgaaacg
     4261 gccaggcgta ttgccgccgc gatgcgctgc cgaattcagt atactgtcac tcgattatta
     4321 ttccccagtt tttgcagaga ccgcgatgtc ggacctgagc tacctgttga acaacgatcc
     4381 cgcgcgcttc gaactgcggc cggacgcgcc gcgcatgatg gcggtggtga ccaccggtaa
     4441 tggcggctac gacaagctcg attaccgtga agtgccggta cccgagcccg gccccggaga
     4501 ggtcctgctg cgcgtgctcg ccgcgggggt caataatacc gagatcaata cccgcctggg
     4561 ctggtattcg tcatcggtca tcagcggcac cgagcagtat gccggcagcg aaccggccgc
     4621 cgacaacctc gccgacggcg gctggaacca ggccacgccg tttccgttca ttcagggtac
     4681 cgattgctgc ggcgaagtgg tggcgctcgg tgacggcgtg gtcggcgaat tcgccggcaa
     4741 gcgggtgctg gtgcgcgcct gcatgcggct gcatggcttc gattcgctgg agaacatctg
     4801 gatggcatcc gatttcgacg gcgccttcgc gcagttcgtc agggtaccgg ccagcgaggt
     4861 gttcaccgtc gactgcaact ggagccacgc cgagctggcg actattccct gtgcctacgg
     4921 caccgccgag aacatggtgc accgcgcggg tgttgcgcgg ggtgaacggg tgctggtcac
     4981 cggtgcttcg ggtggcgtcg gttcggccgt tgtacaactg gcccgccgcc gcggtgcaca
     5041 ggtgatcgcg gtggccggta aggccaaggt cgaagccgtc gcggtgctcg gccccgagcg
     5101 ggtcatcgaa cgcggctgct cgctgctcgg tgaacttggt ccgcgcagcg tcgacgtggt
     5161 g
//
LOCUS       DATCZV010000047         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_321052, whole genome shotgun sequence.
ACCESSION   DATCZV010000047 DATCZV010000000
VERSION     DATCZV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321052"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..243
                     /locus_tag="VIW27_01120"
     CDS             49..243
                     /locus_tag="VIW27_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738289.1"
                     /translation="MAVYKCEVCGMSIGSMTCGTCDKQLEHGSVSGDDGSEIQVSRCP
                     DGHGMIKSPMCCGQDMTRTD"
     gene            455..1081
                     /locus_tag="VIW27_01125"
     CDS             455..1081
                     /locus_tag="VIW27_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY5738290.1"
                     /translation="MSLAAIAGLLGALIVLAIIPSISVLAVSARAAAFGFTHGLFTAI
                     GIVVADILFILIAVYGLVFVAELMGEQFTLVKYLGGVYLIWLGVSLWRADTKTRSSGE
                     IGESSLGSSFLTGFLITLGDQKAILFYLGFFPAFVDLERMTPVDTLLIILIAILGVGG
                     AKLVYAYLADRASLIFKNTRAIRGLNGFAAAVMIMVGVALLLKARQWL"
     gene            1072..1683
                     /locus_tag="VIW27_01130"
     CDS             1072..1683
                     /locus_tag="VIW27_01130"
                     /inference="COORDINATES: protein motif:HMM:NF028177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HEY5738291.1"
                     /translation="MAVSIRLSDLELLDAVRAFALDHPHPDVARFHDSLRHWGDDWRA
                     VEAGYLPAADFLEAAIDDAEPPARELLALFARHRYALRWEQSYRREDGLVPQAMLDGY
                     GFAEIIGQRGPFVSERIRAGIGIWGPSIDYPLHHHRAEEIYLPLAGSAEFRLGDQAAQ
                     TRGHGDVVYVKPELPHGFRTGERCLVVYYLWQAGDLRQTSTFT"
     gene            1779..2051
                     /locus_tag="VIW27_01135"
     CDS             1779..2051
                     /locus_tag="VIW27_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738292.1"
                     /translation="MWLNREQPIGWRPQQGSIERALFDNGAGAAVTDLRIARENRLDG
                     LRIGGQRKLKISPRPACGNRGKAGMIPPDSAIPAEIEFIGDKQYAA"
     gene            2054..>2510
                     /locus_tag="VIW27_01140"
     CDS             2054..>2510
                     /locus_tag="VIW27_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5738293.1"
                     /translation="MSDITDSIKVIAFDVFGTVVDWHGTVKTEVEQMFPGVDGGAFAL
                     AWRAGYQPAMAEVMEKGEWVVLDVLHRRILDRVLAEFGLDDLGEAARHHLNRIWHRLD
                     PWPDSVEGLARLKSKYMVSSLSNGNLGLLAEMAKHAGLPWDIILCAETFN"
BASE COUNT          451 a          808 c          787 g          464 t
ORIGIN      
        1 gggcccaagc tggtaatatc ggcctctcga ctacgcggag gaggatccat ggcagtttac
       61 aaatgcgaag tctgcggcat gagcatcggc agcatgacct gcggcacctg cgacaagcaa
      121 ctggaacacg gcagcgtgag cggcgacgac ggcagcgaga tccaggtttc ccgctgtccc
      181 gacggccacg gcatgatcaa gtcgccgatg tgctgtggcc aggacatgac ccgcaccgac
      241 taaagacgcg ttgaaattct actcagccgg cacgattcag cgttaaaatc gggcgatgaa
      301 tgcgcattta ccagcctgta aactgcgctt tttgcctgcg aggcccggcc cggtctcgcc
      361 ttgcctcggc ccgcggtaga cctgtcgcct gagcggatct caacgcgctg ctagatttgc
      421 cactggttaa aggaggctcg aactgcagtc taccctgtcc ctggcggcga tcgccggatt
      481 gctgggagcg ctgatcgtgc tggcaataat cccgagcatc agcgtgctcg cggtatcggc
      541 gcgcgccgcg gccttcggtt tcacgcacgg cctgttcacg gcgatcggca tcgtcgtcgc
      601 cgacatcctg ttcatcctga tcgcggtcta cggactggta ttcgtcgccg aactgatggg
      661 cgaacagttc acgctggtca aatatctcgg cggggtttac ctgatctggc tcggcgtgtc
      721 gctgtggcgc gccgatacca agacccgcag cagtggcgaa atcggcgagt cctcgctcgg
      781 ctcgagcttc ctgaccggct ttctgatcac cctcggagac cagaaagcga tcctgtttta
      841 tctcggattc ttcccggcat tcgtcgacct cgagcgcatg acgccggtcg ataccttgct
      901 gatcatcctg attgcgatcc tcggcgtcgg cggcgccaag ctggtatacg cgtatctcgc
      961 cgaccgcgcg agcctgatct tcaagaacac gcgcgcgatc cgcggcctga atggcttcgc
     1021 cgcggcggtg atgatcatgg tcggcgtcgc actgctgctg aaagcccggc aatggctgta
     1081 agcattcgcc tgtccgacct cgaactgctc gacgcggtgc gcgctttcgc gcttgaccac
     1141 ccgcacccgg atgtcgcccg ctttcatgat tcgctgcgcc actggggcga tgactggcgc
     1201 gccgtcgagg cgggctattt gccggcggct gattttctcg aggcggcgat cgacgacgcc
     1261 gagccgccgg cgcgggagtt gttggcgctg ttcgcgcgcc atcgatatgc cttgcgctgg
     1321 gagcaaagct accgccgcga agatggcctg gtgccgcagg cgatgctcga cggctacggc
     1381 ttcgccgaaa tcatcggcca acgcggcccg ttcgtcagcg agcgcatacg cgccggcatc
     1441 ggcatctggg ggccgtccat cgactacccg cttcaccacc accgggccga ggaaatctac
     1501 cttccgctgg ccggctcggc cgagttcaga ctcggggatc aggcagcgca aacccgaggc
     1561 catggcgacg tcgtctacgt caaaccggaa ttgccgcacg gctttcgcac cggcgagcgc
     1621 tgcctggtgg tttactacct gtggcaagcc ggcgacctgc ggcagacgtc gaccttcacg
     1681 tgactcgcgt ggcgcgtccc ggtatcgcgc cgttcgatga tcgtcgcggc gatgtcgcac
     1741 cgcggcggcg ccaacacgcc taccgcgtgc gccgctaaat gtggttgaat agagagcagc
     1801 cgatcggttg gcgaccacag cagggttcga tcgagcgcgc actgttcgac aacggcgccg
     1861 gcgccgcggt cacggatttg cgcatcgctc gcgaaaaccg actcgatggt ctgcgcatcg
     1921 gcggccagcg caagctgaaa atctcgcccc gcccagcctg cggcaatcgc ggcaaagcgg
     1981 gtatgatacc gcccgactcg gcgatcccgg ccgagatcga atttatcggg gataagcaat
     2041 atgcggcatg aaaatgagtg acatcacgga cagtatcaag gtaatcgcct tcgacgtttt
     2101 cggcacggtc gtcgactggc acggcacggt caaaaccgaa gtcgagcaga tgttcccggg
     2161 ggtcgacggc ggcgcattcg cgctcgcctg gcgggccggc taccagccgg cgatggccga
     2221 agtcatggaa aaaggcgaat gggtcgtcct cgatgtgctg caccggcgca tcctcgaccg
     2281 ggtactggcc gaattcggcc tcgacgacct gggcgaggca gcgcgccacc atctgaatcg
     2341 catctggcac cgactcgatc cgtggcccga cagcgttgag ggcctcgcgc ggctgaaatc
     2401 gaaatacatg gtgagcagtc tgtccaacgg caacctcggc ctgctcgccg agatggccaa
     2461 gcacgccggc ctgccatggg acatcatcct ctgcgcggaa accttcaacc
//
LOCUS       DATCZV010000048         3962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_744140, whole genome shotgun sequence.
ACCESSION   DATCZV010000048 DATCZV010000000
VERSION     DATCZV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744140"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VIW27_01145"
     CDS             <1..220
                     /locus_tag="VIW27_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738294.1"
                     /translation="WSLFSRGSTEERLSAMAGLVLAAEREQQKYGLRLPSITIEQSFG
                     NAHKHSCLYELAIFRQPESRAELRIESD"
     gene            207..2195
                     /locus_tag="VIW27_01150"
     CDS             207..2195
                     /locus_tag="VIW27_01150"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY5738295.1"
                     /translation="MKATSGNLPIRDIPLEETDRAGVFAVCLCFLFSLAPHFFNLPVW
                     ISAVVVLAIGWRALQNLGLLKPMPKWLLIPLVLAGGIGVFAEYWTIVGRDAGLALLTV
                     MAAFKFLESRNHRDMLILVFLCYFLIATHFLYSQSIPTALLMFATLIIITTTLITLNL
                     RDNGVPVLERLRDGARLVGMSIPLMLVLFVLVPRVPGPLWGLQSEQRGGVTGLSDRMS
                     PGEISNLIRSNEVAFRVDFEGPIPPQSQLYWRGPVMAKYNGYTWFQLPRGRARNLDIY
                     TDDPGVEYTVTLEPNGEQWLLALDIPTRLVKGSLMSKDYQLISRKDINDIFRYSMRSR
                     LDYRIGIDESPQYLARALQYPENSNPRTIELGKSLAERHDSSEAIIDAVLRMYREQPF
                     TYTLQPPALGEDSVDAFLFDTRRGFCEHYAGSFALLMRAAGIPARIVAGYQGGEYNRV
                     GNYLIVRQSDAHAWTEVWIENRGWVRVDPTAAVSPSRIEQGLDDALGAEASGFRIQNR
                     NPIFGNLLYSWDNLQHSWNDWVLNYDQRKQRDFLNQLEIGIETWSDMVFALVILLISV
                     TALFWLVVWYRERPARPAVYELLFRRMLSKLSKRGYAREPWEDTRDFVARLSQCGLPE
                     YDQVAQIVDLYNQIKYGRRGASTQAVKRLRTAVYSLRL"
     gene            2334..2759
                     /locus_tag="VIW27_01155"
     CDS             2334..2759
                     /locus_tag="VIW27_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738296.1"
                     /translation="MRILVSSLLLIVSGCSAQQNADNDPVSREQALAETDPWHTVRLH
                     GVAFRALGQEPGWLLEIKNGEEILIVTNYGQHRVAHAYVEPQQGKAAHRITFKVDADT
                     NLLIEGKPCTDSMSGEQFETTVTMKTGIQTLQGCGRTRF"
     gene            2786..3124
                     /locus_tag="VIW27_01160"
     CDS             2786..3124
                     /locus_tag="VIW27_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738297.1"
                     /translation="MKSTRLPALLILLVGCATSAQAERLRGEYTFGHEVNIFCPQNGS
                     QCFWLGAHSSQAARERLREIYRDKNPGLYKPICVIVDGVIDRASARDGFAADYDGLID
                     IEAVVGPCPD"
     gene            complement(3325..>3962)
                     /locus_tag="VIW27_01165"
     CDS             complement(3325..>3962)
                     /locus_tag="VIW27_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY5738298.1"
                     /translation="LDEHGVRVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLREG
                     LDMPEVSLVAILDADKEGFLRSDRSLIQTIGRAARNLNGMSILYADVVTGSMRRAIDE
                     TERRREKQIAYNEEHGITPRGIVKGISDVMDVGEAGRPGRHRKRFDRVAEPAADFAHL
                     SATQLNARLKKLENSMYEHARNLEFEEAARVRDEIAAIKESYFKSSELEIA"
BASE COUNT          751 a         1230 c         1218 g          763 t
ORIGIN      
        1 ctggtcgctg ttctcgcgcg gctccaccga ggaacgcctg agcgcgatgg ccggactggt
       61 gctggccgcc gagcgtgagc agcagaaata tggtttgcgg ctgccgtcga ttaccattga
      121 gcaaagcttc ggcaatgccc acaagcattc atgcctgtat gaactggcga tcttcaggca
      181 gcccgaatcg cgagcggagt tgcggattga aagcgactag cggcaacctg ccgattcgag
      241 atattccgct cgaggagacg gaccgtgccg gtgtgtttgc ggtctgcctg tgtttcctgt
      301 tctcactggc gccgcatttc ttcaatctgc cggtgtggat cagcgccgtc gtcgtgctcg
      361 cgatcggctg gcgggcgcta cagaatctcg gcctgctgaa accgatgccc aaatggctgc
      421 tgattccgct ggtgctggcc ggcggtatcg gcgtattcgc cgagtactgg accatcgtcg
      481 gacgcgacgc ggggctcgcg ctgctgaccg tgatggccgc gttcaagttt ctcgaaagcc
      541 gcaaccaccg cgacatgctg atcctggttt ttctgtgcta cttcctgatc gcgacgcatt
      601 tcctctactc gcaaagcatt cccaccgcgc tgttgatgtt cgcgaccctg atcatcatta
      661 ccacgacgct gatcacgctc aacctgcgcg acaacggcgt gccggtgctc gaaaggctgc
      721 gcgacggcgc caggctggtc ggcatgtcga ttccgctgat gctggtattg ttcgtgctgg
      781 tgccgcgggt accgggcccg ctgtggggcc tgcagagcga acagcgcggc ggcgtgaccg
      841 gcctctccga ccgcatgtcg ccaggcgaga tcagcaatct gatccgcagc aacgaagtcg
      901 cctttcgggt cgatttcgag ggcccgattc cgccgcagag ccagctctac tggcgcggtc
      961 cggtgatggc gaaatacaac ggctatacct ggttccagtt gccccggggg cgggcgcgca
     1021 acctcgatat ctataccgac gacccgggtg tcgagtacac ggtcaccctc gaacccaacg
     1081 gcgaacaatg gttgctggcg ctggatattc cgactcgcct ggtcaagggc agtctgatgt
     1141 ccaaggatta ccagttgatc agcagaaagg atatcaacga catctttcgc tactcgatgc
     1201 gctcccgcct cgattaccgg atcggcatcg acgaatcgcc ccagtacctg gcacgggcgc
     1261 tgcagtaccc cgagaattcg aacccgcgca ctatcgagct cggcaaatcc ctcgccgaac
     1321 gccacgacag cagcgaggcg atcatcgacg cggtattgcg catgtatcgc gaacagcctt
     1381 tcacctatac cctgcagccg ccggctctgg gcgaggactc ggtcgacgcg ttcctgttcg
     1441 atacgcgccg cggcttctgc gagcattacg cgggcagctt cgcgctgctg atgcgcgccg
     1501 ccggcatacc cgcgcggata gtcgccggct accagggcgg cgaatacaac cgggtcggca
     1561 attacctcat cgtacgccag tcggatgccc acgcctggac cgaggtgtgg atcgagaacc
     1621 gcggctgggt tcgcgtcgac ccgaccgccg cggtatcgcc gagccgtatc gagcagggtc
     1681 tcgacgatgc gctgggcgcc gaggccagcg gatttcgcat tcagaatcgt aacccgatct
     1741 tcggcaacct gctgtacagc tgggacaacc tgcagcacag ctggaacgac tgggtgctga
     1801 attacgacca gcgcaaacag cgcgactttc tcaaccaact cgaaatcggc atcgaaacct
     1861 ggtccgacat ggtatttgcg ctggtcatac tgctgatttc ggtcaccgcg ctgttctggc
     1921 tggtcgtctg gtaccgcgag cgtccggcaa ggccggccgt ttacgaactc ctgttcaggc
     1981 ggatgttgag caagctctcc aaacgcggct acgccagaga accctgggaa gacacccgcg
     2041 acttcgtcgc ccgcctgtca caatgcggtc tgcccgagta cgatcaggtc gcgcagatcg
     2101 tcgatctgta caaccagatc aaatacgggc gccgcggcgc gtcgacgcag gcggtcaagc
     2161 ggttgcgaac cgccgtctat tcgctgcgcc tgtaaatacg gccggcggta atgcggggca
     2221 accaggttcc gttcgcagca gtttgcggct gagctgcaaa gccggctagc gggtcgcttc
     2281 gcgcgataaa gaaagacggc ggggattacg gcatcaagtt gtagaatcgg gccatgcgca
     2341 tcctcgtatc gtcactactg ctgatcgtta gcggctgcag cgcccagcaa aacgctgaca
     2401 acgaccctgt cagcagagag caggcgcttg ccgaaaccga cccgtggcac acggtcaggc
     2461 tgcacggcgt cgcatttcgc gcgctcggcc aggagcccgg ctggctgctg gagatcaaga
     2521 acggcgagga aatcctgatc gtcaccaatt acgggcagca ccgggttgcc catgcctacg
     2581 tcgagccgca gcaaggcaag gccgcgcaca ggataacctt caaggtagac gccgacacca
     2641 acctgctgat cgagggcaaa ccctgcacgg attcgatgag cggcgaacag tttgaaacta
     2701 cggtgaccat gaagaccggc atccagacgc tgcaaggctg cggccgtacc cggttttgaa
     2761 acctgtaccg aatgcgccct acgctatgaa atctacacga ctgccggcct tgctcattct
     2821 gctcgtcggc tgcgcgacga gtgcgcaggc ggaacgtttg cgtggcgagt ataccttcgg
     2881 acacgaagtc aatatctttt gcccgcaaaa cggctcgcag tgtttctggc tcggagcgca
     2941 cagcagccag gccgcacgcg agcgccttcg cgaaatctac cgggacaaga atccgggcct
     3001 gtacaagccg atctgcgtga tcgtcgacgg cgtgatcgac cgcgcttcgg cacgcgacgg
     3061 cttcgccgcc gactacgatg gtctgatcga tatcgaggcg gtggttggcc cctgccccga
     3121 ctgaagcggc aggatgcagc gtcgttgacg atcagatgtt attgtcatgc cggccctcga
     3181 gccggcatcc atcgggaagc cttcgagata gccgccgggc gaagcccggc gaaaactgcg
     3241 agtcattgaa atggattgcg ggtcgagccc gcaatgacag cagcggcgtc atgccggccc
     3301 tcgagcccgc aatgacggga tttgtcaggc gatttcgagt tcggaggact tgaagtagct
     3361 ttccttgatc gccgcgatct cgtcgcgcac gcgcgcggct tcctcgaact cgaggttgcg
     3421 cgcgtgctcg tacatgctgt tttcgagttt cttcagccgc gcattcagct gggtagcgct
     3481 caggtgggcg aaatcggccg ccggctcggc gacgcggtcg aaacgcttgc ggtgacggcc
     3541 cgggcgaccc gcctcgccga catccatgac atcgctgatg cctttgacga tgccgcgagg
     3601 cgtgatgccg tgttcttcgt tgtaggcgat ctgtttctcc cggcgacgtt cggtttcgtc
     3661 gatcgcgcgc cgcatcgagc cggtgaccac gtcggcatag agaatcgaca tgccgttgag
     3721 gttgcgcgcc gcgcggccga tggtctggat caacgagcga tcggagcgca gaaaaccttc
     3781 cttgtcggcg tcgagaatcg ccaccagcga cacttccggc atatcgagac cctcgcgcag
     3841 caggttgatg ccgaccagca cgtcgaattc gccgagcctg aggtcgcgaa tgatttccat
     3901 gcgctcgacg gtatctatat ctgaatgcag gtaacgcacg cgcacgccgt gctcgtcgag
     3961 at
//
LOCUS       DATCZV010000049         6401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_533161, whole genome shotgun sequence.
ACCESSION   DATCZV010000049 DATCZV010000000
VERSION     DATCZV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533161"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="VIW27_01170"
     CDS             <1..1632
                     /locus_tag="VIW27_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5738299.1"
                     /translation="GCNRNNVDDFVGINAVEQYIGDTAKEQGFTFGDLPEVGDKRVAI
                     VGGGPAGLAAAYQLRRKGNASTIFDEHDELGGMFRYGIPGYRTPREFLDHEIRRILDM
                     GHIDTRMATRVGRDVSVDDLEKEFDAVLWAIGCQTGRGLPLEHGDAPNCIAGVAFLEA
                     FNRGALQVTADRVVCVGGGDTSIDVVSVARRLGKIDKLPKEQLTEFVVGGFTAHDSAY
                     AAMRQGADVTLTSLFERAEMTATEHEVEDALREGVKILDGVMPIEIIVNEDGRATALK
                     MAKCKIDNGVPKVVEGSEFQLECDLIVSAIGQGGNLEGIEALGNERNLMDADKYFRHT
                     GKSGHFVAGDIIHPHLLTTAIGHASVAADSIERYLGGAEFDKRPKVDVHHFNLLGKLK
                     EMHLEPEEFPHQPLWGTNDAKFAVHNYEDRSANEIISTERMFLAHFNYADRHQREMHG
                     PDSEQVLGDFSERTRSLDEQQAVDEAGRCMSCGMCFECDNCVIYCPQDAVYRVKKDER
                     TMGRYVATDYTRCIGCHICADVCPTGYIDMALGDH"
     gene            1638..2144
                     /locus_tag="VIW27_01175"
     CDS             1638..2144
                     /locus_tag="VIW27_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738300.1"
                     /translation="MAATGILRFSLLLAAWLALTPLLLPAAARAEGSAFGAVSIPQPA
                     SPTQAENCVEPVEIMRREHMHLLDQQRDRTVIDGERDGKHSLVGCMDCHNPVDANGQA
                     ASYGDAQHFCSECHAYASVKIDCFECHADRGYGAASGGVSANAWDDDGTALTAATLQH
                     SLEAADVD"
     gene            2134..2883
                     /locus_tag="VIW27_01180"
     CDS             2134..2883
                     /locus_tag="VIW27_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5738301.1"
                     /translation="MSTDGTGHDDKRRGFLQGAAALAATMVAPGVFLYQAGEAASSLQ
                     AGVRWGMLIDNNLLDESDVDACVTACARENGWQAEPGSTTDAQWIRKVRLTDRSSGYR
                     SFLPLMCQHCASPPCVDVCPTGASFKRADGIVLVNKHTCIGCRYCMMACPYKARSFIH
                     EKLDNQQPHAPRGKGTVESCTLCVHRIDRDGANAVPACAEAVNRDGRKAILFGDLNDA
                     DSEISRRLREFGSLRLRADMGLEPAVSYSGI"
     gene            2886..4082
                     /gene="nrfD"
                     /locus_tag="VIW27_01185"
     CDS             2886..4082
                     /gene="nrfD"
                     /locus_tag="VIW27_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY5738302.1"
                     /translation="MQTTRFAEIRTRSTTYRALLLALLAFVLAGLGAAHHMDVEGHHV
                     TGMTNQIVWGLPHVFAIFLIVAASGALNVASLSSVFDQAAYRPLARFSAILSIALLFG
                     GLAILLLDLGRPDRLIVAMTHYNFKSIFAWNILLYVGFFVIVAVYLWLMMERRMYRYS
                     RPAGIVALVWRLILTTGTGSIFGFLVAREAYDTAILAPLFVILSFAIGLAVLVLLLLM
                     SFVLDGRELDTSLLKRLARLLGIFTAAVFYFVLVFHLTKLYGTQNHEFERFILLDGGL
                     YSALFWIVQIGFGNLLPMALVFAPQLQGSPRALAAACALVIAGGFAQLYVVIIGGQAY
                     PLNIFPGYLVDSSFHDGTVNAYLPSAWEWLLGFGGLTAAILAVLLIVRVLPILPESLR
                     SEESGA"
     gene            4079..5482
                     /locus_tag="VIW27_01190"
     CDS             4079..5482
                     /locus_tag="VIW27_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEY5738303.1"
                     /translation="MSSVFLSAAHKSSGKTTFAIGLCAVLRQRGLAVQPFKKGPDYID
                     PAWLGLAAGRPCYNLDYFVAGEHETIGEFARRGANADISLIEGNKGLHDGLDLDGRNS
                     NAALAKAVRSPVILVLDVRGTIRGIAPLLIGYQIFDREVDIAGVVANLCGGPRHESKL
                     RAAVDAYTDIPFLGCLANDPELTLDERHLGLVPGYEDVAARRKIERIADAVRADIDVD
                     RILEIAQRAGPLDAPALPLRLPRRFDGLRIGIARDRAFGFYYAGDLDAFAAAGAELVA
                     IDTLNDTALPKLDGLLIGGGFPERHAAELAANQVMRAAVHDAIEAGLPTYAECGGLMY
                     LSQSLTWNGETYPMTGVLPARTQMHDKPQGRGYVILEAGETQHPWLPKGYAATGVNAH
                     EFHYSSLHGLPADSRYAYHVRRGSGISEQRDGLIYKNLFASYCHLRNTGGTPWVESFL
                     QFVARHKRPLHRAVGAD"
     gene            5486..5800
                     /locus_tag="VIW27_01195"
     CDS             5486..5800
                     /locus_tag="VIW27_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEY5738304.1"
                     /translation="MIHLTTAALEQIRESANQEGFASMPLRIAIKEQEDGSFHYAMGF
                     DEQRLPGDTFYNFDGVDLVVATASKNLSEGMTIDFVELEPGKPEFIFLNPNDPTYVPP
                     KS"
     gene            5884..>6401
                     /locus_tag="VIW27_01200"
     CDS             5884..>6401
                     /locus_tag="VIW27_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738305.1"
                     /translation="MSNASPKPPRNTNPFNAWFVKLDEENRGLLRSGRFRLLLLSTVL
                     LALLTVGLLGSLFSGQPLFAAIDEHSAWILGLSLLLVVALLWEVWRELLQPLLRLCDW
                     ADLMRGVNLDARVSMPADSDFTELARDINMLGNMIQNLSRDTEIQLQKHTDHITRESR
                     SLAILYDVASSI"
BASE COUNT         1137 a         2158 c         1940 g         1166 t
ORIGIN      
        1 ggctgcaacc gcaataacgt cgacgacttc gtcggcatca acgcggtcga gcagtacatc
       61 ggcgataccg cgaaggaaca gggcttcacc ttcggcgacc tgccggaggt cggcgacaag
      121 cgtgtcgcca tcgtcggcgg cggcccagcg ggcctcgccg ccgcctacca gctgcgccgc
      181 aagggtaacg ccagcaccat cttcgacgag cacgacgagc tcggtggcat gttccgctac
      241 ggcattcccg gctaccgcac gccgcgtgaa tttctcgatc acgaaatcag gcgcatcctc
      301 gacatgggcc atatcgatac ccgcatggcg acccgcgtcg gccgcgatgt cagcgtcgac
      361 gacctggaaa aggaattcga tgcggtgctg tgggcaatcg gctgccagac cgggcgcggc
      421 ctgccgctgg agcacggcga tgcacccaac tgcatagccg gcgtcgcttt tctcgaagct
      481 ttcaaccgcg gtgcgctgca ggttaccgcc gaccgcgtgg tctgcgttgg cggcggcgat
      541 acctcgatcg acgtggtctc ggtggcgcgc cgccttggca agatcgacaa gctgcccaaa
      601 gagcaactga ccgagttcgt ggtcggcggt ttcaccgcgc acgactccgc ctacgcggcg
      661 atgcgccagg gcgccgacgt cacgctgact tctctgttcg agcgcgccga gatgaccgcc
      721 accgagcacg aggtcgagga tgcgctgcgc gagggcgtga agattctcga cggcgtgatg
      781 ccgatcgaaa tcatcgtcaa cgaagacggc cgcgcgaccg cgctgaaaat ggccaaatgc
      841 aagatcgaca acggcgtgcc caaagtggtc gagggcagcg agttccaact cgaatgcgac
      901 ctgatcgtgt ccgctatcgg ccagggcggc aacctcgagg gaatcgaagc actgggcaac
      961 gagcgcaacc tgatggatgc cgacaagtat tttcgccaca ccggcaaatc cggccacttc
     1021 gtcgccggcg acatcattca cccgcacctg ctgaccaccg ctatcggcca cgcatcggtc
     1081 gctgccgaca gcatcgagcg ctacctcggc ggcgccgagt tcgacaagcg gcccaaggtc
     1141 gacgtgcatc acttcaacct gctcggcaag ctcaaggaaa tgcatctcga acccgaggaa
     1201 tttccgcacc agccgttgtg gggcaccaac gacgcaaaat ttgcggtaca caattacgaa
     1261 gaccgttcag ccaacgaaat catttccacc gaacgcatgt ttctcgcgca ctttaattac
     1321 gccgatcgtc accagcgcga gatgcacggg ccggattccg agcaggtgct cggcgacttt
     1381 tccgagcgta cccgcagcct cgacgagcaa caggcggttg acgaagccgg ccgctgcatg
     1441 agttgcggca tgtgtttcga atgcgacaat tgcgttatct attgcccgca ggacgccgtt
     1501 taccgggtca agaaagacga acgcaccatg ggtcgctacg tggccaccga ctacacgcgc
     1561 tgcatcggct gccatatttg cgccgacgtc tgcccgaccg gttacatcga catggcgctc
     1621 ggtgatcact gacatttatg gctgcaaccg gcatcctgcg tttctccctg ctgctcgccg
     1681 cctggctggc gctgaccccg ctgctgctgc ccgccgccgc ccgggccgaa ggttccgcgt
     1741 tcggcgcggt ctcgattccc cagcccgcca gtcccacgca ggcggaaaat tgcgtcgaac
     1801 cggtggaaat catgcgccgc gagcacatgc acctgctcga ccagcagcgc gaccgcacgg
     1861 tgatcgatgg cgagcgcgac ggcaagcaca gcctggtcgg ctgcatggat tgccacaatc
     1921 cggtcgacgc caacgggcaa gccgccagct acggtgatgc gcagcatttc tgcagtgaat
     1981 gtcacgccta tgccagcgtc aagatcgact gcttcgaatg ccacgccgat cgtggctatg
     2041 gtgcagcgtc aggcggcgtt tctgctaacg cctgggacga cgacggcacc gcgctgaccg
     2101 cagccaccct gcagcacagc ctggaggccg ccgatgtcga ctgacggcac aggtcacgac
     2161 gataagcgcc gcggttttct gcaaggcgcc gccgcgctcg ccgctacaat ggtggcgccg
     2221 ggtgtgtttc tttaccaggc gggtgaggcg gcctcttccc tgcaggctgg cgtacgctgg
     2281 ggcatgctga tcgacaacaa cctgctcgac gagagcgatg tcgacgcctg cgtcacagcc
     2341 tgcgctcgcg agaacggctg gcaggccgag cccggttcga ccaccgacgc gcagtggatt
     2401 cgcaaggtca ggctcaccga ccgcagtagc ggttaccgca gcttcctgcc gctgatgtgc
     2461 cagcattgcg cgagcccgcc ctgcgtcgat gtctgcccga ccggcgcctc cttcaagcgc
     2521 gccgacggca tcgtgctggt caacaagcat acctgcatcg gctgccgcta ctgcatgatg
     2581 gcctgcccgt acaaggcgcg ctcgttcatc cacgaaaaac tcgataacca gcagccgcac
     2641 gcgccgcgcg gcaagggcac cgtcgaatcc tgcacgctgt gtgtgcatcg catcgaccgc
     2701 gacggcgcca atgcggtgcc ggcctgcgcc gaggccgtca accgcgacgg ccgcaaggcg
     2761 atcctgttcg gcgatctcaa cgatgccgac agcgaaatct cgcgccgcct gcgcgagttc
     2821 ggcagcctgc gactgcgcgc cgatatgggc ctcgagccgg ccgtcagcta ctcgggtata
     2881 tagacatgca gacgacgcgc ttcgccgaga tcagaacccg cagcacgact tatcgcgcgc
     2941 tgctgctggc gctgctggca ttcgtgctgg ccggactcgg cgcggctcac cacatggatg
     3001 tcgaggggca ccacgtcacc ggcatgacca accagatcgt ctggggcctg ccgcacgtgt
     3061 tcgcaatttt cctgatcgtc gccgcctccg gcgcgctcaa cgtcgcctcg ctgtcgtcgg
     3121 tgttcgacca ggccgcctac cggccgctgg cgcgcttctc ggcgatcctg tcgattgcgc
     3181 tgctatttgg cggactggcc atcctgctgc tcgacctcgg ccgccctgac cgcctgatcg
     3241 tggcgatgac acactacaac ttcaaatcga ttttcgcctg gaacatactt ctctatgtcg
     3301 gtttcttcgt catcgttgcc gtctacctgt ggctgatgat ggaacggcgc atgtaccgct
     3361 acagcaggcc tgccggtatc gtggcgctgg tctggcggct gatactgacc accggtaccg
     3421 gctcgatctt cggcttcctg gtggcgcgcg aggcttacga caccgcgatt ctcgcaccgc
     3481 tgttcgtgat cctgtcgttc gccatcggcc tggcagtgct ggtgttgctg ctgttaatga
     3541 gcttcgtgct cgacgggcgc gagcttgata cctcgctgct gaagcgcctc gcgcggctgc
     3601 tcggaatctt caccgccgcg gtgttctact tcgtgctggt gtttcacctg accaagctct
     3661 acggcaccca gaaccacgaa ttcgagcgtt tcatcctgct cgatggcggt ctctacagcg
     3721 ccctcttctg gatcgtgcaa atcggcttcg gcaacctgct gccgatggcg ctggtgttcg
     3781 cgccgcagct gcaggggtcg ccccgtgccc tggctgccgc ctgcgcgctg gtcatcgccg
     3841 gcggcttcgc gcaactgtat gtggtcatta tcggcggcca ggcctacccg ttgaacatct
     3901 ttcccggtta cctcgtcgac agcagtttcc acgatggcac ggtcaacgcc tacctgccga
     3961 gcgcctggga atggctgctc ggcttcggcg gcctgaccgc ggcgattctc gccgtgctgc
     4021 tgatcgtccg cgtgctgcca atcctgccgg agtcgctgcg cagcgaggag tccggggcat
     4081 gagttcggta tttctgtcgg cagcccataa gtcttccggc aagaccacct tcgccatcgg
     4141 cctgtgcgcc gtactgcgcc aacgcggcct cgccgtgcag cctttcaaga agggacccga
     4201 ctacatcgat ccggcctggc tggggctggc tgccggcagg ccctgttaca acctcgacta
     4261 ttttgtcgct ggcgagcacg aaaccatcgg cgaattcgca cggcgcggcg cgaatgccga
     4321 catttcgctg atcgaaggta acaagggcct gcacgacggg ctcgacctcg acggcagaaa
     4381 cagcaatgct gcgctggcca aggcggtccg cagcccggta atcctggtcc tcgacgtgcg
     4441 cggcacgatc cgcggtatcg cgccgctgtt gatcggctac cagatattcg accgagaggt
     4501 cgatatcgcc ggcgtcgtcg ccaacctgtg cggcggtccg cgccacgaat ccaagctgcg
     4561 cgccgcggtc gacgcctaca ccgatattcc gtttctcggc tgcctggcga acgacccgga
     4621 gctgactctc gacgagcgcc atctcggcct ggtgccgggc tacgaggatg tcgcggcgcg
     4681 ccgcaaaatc gagcgtatcg ccgatgcggt gcgtgccgat atcgacgtcg accgcatcct
     4741 cgagatcgcg cagcgggcgg gtccgctcga cgcccccgcc ctgccgttga gactgccgcg
     4801 ccgtttcgac ggcctgcgca tcggcatcgc ccgtgaccgc gcgttcggct tttactatgc
     4861 cggggacctc gacgcctttg ccgcggcggg cgcggaactg gtcgccatcg acaccctcaa
     4921 cgataccgca ctgccaaagc tggacggcct gctgatcggc ggcggtttcc ccgagcgcca
     4981 cgccgccgag ctggctgcca accaggtcat gcgcgcggcg gtccacgacg ccatcgaggc
     5041 gggcctgccg acctacgccg agtgcggcgg cctgatgtac ctgtcgcaaa gccttacctg
     5101 gaacggcgaa acctacccga tgacaggcgt tctgccggcc cgcacgcaaa tgcacgacaa
     5161 gccgcagggc cgcgggtacg tgatcctcga agctggcgag acgcagcacc cgtggctgcc
     5221 gaaaggttac gcggccaccg gtgtcaacgc gcacgaattt cactattcct cgctgcacgg
     5281 attacctgcc gacagccgat atgcctatca cgtgcggcgc ggcagcggca tcagcgaaca
     5341 gcgcgacggg ctgatctaca agaatctgtt tgcctcctat tgccatctgc gcaataccgg
     5401 cggcacgccg tgggtcgaga gctttctgca attcgtcgcg cggcacaagc gaccgctgca
     5461 tcgcgccgtc ggggcggact gagcgatgat ccacctgacc accgcggcgc tcgagcaaat
     5521 cagggaatcg gccaaccagg aaggcttcgc ttcgatgccg ctaagaatcg cgatcaagga
     5581 gcaggaagac ggcagcttcc attacgcgat gggcttcgac gagcaacgcc tgcccggcga
     5641 caccttctac aacttcgacg gcgtcgacct ggtggtggcg acggccagca agaacctctc
     5701 cgagggcatg accatcgatt tcgtcgaact cgaacccggc aagcccgaat tcattttcct
     5761 caatccgaac gatcccacct acgtgccgcc aaaaagctag cccggcaagt ccgcgctaaa
     5821 tggcgttgaa cgcggtatac tggcgccgat agtgattcac gggaccgcca ttcgattcgc
     5881 cccatgtcca acgccagccc caagccaccg cgtaatacca acccgttcaa cgcctggttc
     5941 gtcaagctcg acgaggaaaa tcgcggcctg ctgcgcagtg gccgctttcg actgctgctg
     6001 ctgtcgacgg tcctgctggc gctgttgacc gtcgggctgc tgggatcgct gttttcgggc
     6061 cagcccttgt tcgccgccat cgatgagcac tcggcctgga tactgggcct gtcgctgctg
     6121 ttggtggtcg cgctgctgtg ggaggtctgg cgcgaactgc tacaacccct gctgcgactt
     6181 tgcgactggg cggatctgat gcgcggggtc aatctcgatg cccgggtcag catgcccgcg
     6241 gacagcgatt tcaccgaact cgcccgcgat atcaacatgc tcggcaacat gatccagaat
     6301 ttgtcgcgcg ataccgagat ccagctgcag aagcataccg accacattac ccgcgaatcg
     6361 cgctcgctgg cgatactcta cgatgtcgcc tcgagcatca a
//
LOCUS       DATCZV010000050         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_268304, whole genome shotgun sequence.
ACCESSION   DATCZV010000050 DATCZV010000000
VERSION     DATCZV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268304"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VIW27_01205"
     CDS             complement(<1..504)
                     /locus_tag="VIW27_01205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5738306.1"
                     /translation="MSYPDFCSADFKSSPYWWDRTPRPTREDATLPAKTDVLIVGSGY
                     TGLHCALETARAGRETLVVDAEDAGWGCSTRNGGQISGAIKPDFDTLSRRYGREQAKA
                     LIGEARGALRWLGEFVDREGIDCDYRRCGRYLAAHNPRQFDRLVSQARNQPAGLEQPL
                     RIVERWQQ"
     gene            709..1617
                     /locus_tag="VIW27_01210"
     CDS             709..1617
                     /locus_tag="VIW27_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5738307.1"
                     /translation="MESSKATILIVDDEPINIDTICAHLESDGFDLVTATDGQQAMRL
                     LESNPSRYDVVILDRAMPGIDGMQVLGNIKENESTERIPVLMQTARDASQEVVEGLRA
                     GAYYHLTRPYDGEVLKTVVRSAVNNRIHEKSLHESLRQGRRIFDLMSFGRFRMRDLHE
                     CNLVASQVAHLCPEPYRVVTGISELLINGLEHGNLGIGYEEKNHLIRSGMWRLEIERR
                     LGASDNRGRFVELEFTVQPDCVQIRIRDQGRGFDWAPYMTLDPQRAFDPHGRGIAVAR
                     LSSFDKLEYIGSGNEVLATVDKPKRS"
     gene            complement(1624..>2244)
                     /locus_tag="VIW27_01215"
     CDS             complement(1624..>2244)
                     /locus_tag="VIW27_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEY5738308.1"
                     /translation="IVFTSDHGEQMGDHWLMSKLGYFDQSYHVPLIIRDPSPAADAVR
                     GRQIDAFTESVDLMPTLLDWLGLEIPSQCDGFSLLPALRSGSLPRGWRDEAHWEFDFR
                     NVRDARVERHFGLTLHQCGLAVIRDTRYKYVHCSALPPLFFDLERDPGEFENQAENPQ
                     YRSRVLEYAQRMLSWKMNHTDRGLTETMLGEGGAVTRRAPPRNLDS"
BASE COUNT          417 a          723 c          712 g          392 t
ORIGIN      
        1 ctgctgccag cgctcgacga tgcgcagcgg ttgctcgagg ccggccggct ggtttctcgc
       61 ctggcttacc aggcggtcga actggcgcgg gttatgcgcg gcaaggtagc ggccgcagcg
      121 tcgatagtcg cagtcgatgc cctcgcggtc gacgaattcg ccgagccacc gaagcgcgcc
      181 gcgcgcctcg ccgatcagcg ccttcgcctg ctcgcggccg tagcggcggc tcagggtatc
      241 gaaatccggc ttgatggcac cactgatctg accgccattg cgcgtgctgc atccccagcc
      301 cgcatcctcg gcgtcgacaa ccagcgtctc gcggccggca cgcgcggttt cgagcgcgca
      361 atgcaggccg gtatatccag agccgacgat cagcacatcg gttttcgccg gcagcgtcgc
      421 atcctcgcgc gtcggccgcg gcgtgcgatc ccaccagtaa ggactgctct tgaagtccgc
      481 gctgcagaaa tccgggtagc tcatctatcg attcccgtcg ccgccggcgg cgaggcgcag
      541 agtcgaattc tgtgacggtg ttaaaggttc gacggtcggc ataagttgct ataattcggt
      601 ttggcgatcg ccaatggccg gggcaattta caatactcgg cgggtaaatg acagcggctt
      661 gcacgggcag cgccgcgatc gccggaattc gggggaacaa gaacgccaat ggagagttcg
      721 aaggccacga tattgatcgt cgacgacgag cctatcaata tcgataccat ctgcgcacac
      781 ctggaaagcg acggcttcga cctggtcacc gcgaccgacg gccaacaggc catgcgtctg
      841 ctggaatcga atccatcgcg ttacgacgtc gtcatcctcg accgcgcgat gcccggcatc
      901 gatggcatgc aggtgctcgg gaacatcaag gaaaacgaat cgaccgagcg cattccggtg
      961 ttgatgcaaa ccgcgcgaga tgccagccag gaagtggtcg agggattgcg cgccggcgcc
     1021 tattaccacc tcacccggcc ctatgacggc gaagtgctga agaccgtggt gcgcagcgcg
     1081 gtcaataacc gcattcacga aaagtcgctg cacgaatcgc tgcgccaggg gcggcgcatc
     1141 ttcgacctga tgtcgttcgg acggtttcgc atgcgcgacc tgcacgagtg caacctggtc
     1201 gcctcgcagg tagcacacct gtgccccgag ccctatcgcg tggttaccgg catctccgag
     1261 ctgctgatca acgggctcga gcacggcaat ctcggcatcg gctacgagga gaagaaccac
     1321 ctgattcgca gcggcatgtg gcgcctggag atcgagcgac gcctgggtgc cagcgacaat
     1381 cgcggccgtt tcgtcgaact cgagttcacc gtccagcccg attgcgtgca gatccggatc
     1441 cgcgaccagg gccgcggttt cgactgggcg ccctacatga ccctcgatcc gcaacgcgcc
     1501 ttcgacccgc acggtcgcgg tatcgcggtg gcgcgcctgt cgagtttcga caaactcgaa
     1561 tacatcggca gcggcaacga ggtgctggcc accgtcgaca agccgaaacg cagttagacg
     1621 gcttcagctg tcgagattgc gcggcggtgc gcggcgggtc accgcgccac cctcgccgag
     1681 catggtctcg gtaagcccgc ggtcggtgtg gttcattttc cacgacagca tgcgttgcgc
     1741 gtactcgagt acacgcgacc ggtattgggg attctctgcc tggttttcga attccccggg
     1801 atcccgctcg agatcgaaaa acagcggcgg cagcgcgctg cagtgcacgt acttgtaacg
     1861 cgtgtcgcga atcaccgcca gcccgcactg gtgcagcgtc agcccgaaat gccgctcgac
     1921 gcgagcgtcg cgcacgttgc gaaaatcgaa ttcccagtgc gcctcgtcgc gccagccccg
     1981 cggcaggctg ccgctgcgca gcgccggcag cagcgaaaat ccgtcgcact ggctcggaat
     2041 ctcgaggcct agccagtcga gcaaggtcgg catcaggtcg acgctctcgg tgaacgcgtc
     2101 gatctgccgg ccgcgcacgg catcggcggc cgggctcggg tcgcgaatga tcagcggcac
     2161 gtggtaggac tggtcgaagt agccgagctt cgacatcagc cagtggtcac ccatctgctc
     2221 accatgatcg ctggtgaaga cgat
//
LOCUS       DATCZV010000051         5342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_427363, whole genome shotgun sequence.
ACCESSION   DATCZV010000051 DATCZV010000000
VERSION     DATCZV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427363"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VIW27_01220"
     CDS             <1..711
                     /locus_tag="VIW27_01220"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY5738309.1"
                     /translation="AIIRFVGLEATITDVFARYRWLDGSETTAIARPSQPWIEVATQR
                     GAWQVAGDYTLLGIDHILSGFDHLAFVLALLLIVSGARRLLITVTSFTVAHSITLAAA
                     TLGVLWVPGPPIEAVIALSILFLAGELVKINRGEASLTARNPWIVAFAFGLLHGFGFA
                     GALGEIGLPQNEIALALLMFNVGVELGQLLFIAAALAVIWLLQRLRLQWPDWAQQLPA
                     YGIGGLAAFWVIERVAGF"
     gene            complement(853..2382)
                     /locus_tag="VIW27_01225"
     CDS             complement(853..2382)
                     /locus_tag="VIW27_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEY5738310.1"
                     /translation="MTDSVEQGNSKSGFGSPLLIVALLMTGAIAIWGLLDTAGLAAIA
                     AKLVHIQFVSRGWFVMLAVSFMLLVSIWLALSRYGGIKLGRDDDEPEFSTVSWLTMLF
                     AAGMGVGLLFWGSAEPLTHFELIRQNHSPGRAADMALFVTNFHWGLHAWAIYALTGLV
                     IAYFGFRRGCPSLIGSPIVDTFGRNRFTGSFSWLANLLAIVAIAIGVGGSIAMGVFQV
                     KDGLDTLFDLRDTGLGMTTAVFVVLCIAYFPPLVVDLGSGMARLSNTAMAIAGGLMVF
                     ILLAGPTHYLMGGITQAIGEYISNVLPHGFRTFTFMDEQVGNWFQSWTLTYMVWWIAW
                     APFVGVFIARISRGRTIREFIVGVILGPTVFSLLWFGIFGGAGFYEVLRRGSAIMDVV
                     ENNVSAVTFFMLESFPLPLLTISLVIVAAFLFIVTSVVSAAFVLGMFSSNGELDPSAR
                     IKLAWGVILGALGYVMILSGDIAAIKSIIALGSLPFVFVVLVLVVCLLKTLKHEPEGV
                     S"
     gene            complement(2620..3654)
                     /locus_tag="VIW27_01230"
     CDS             complement(2620..3654)
                     /locus_tag="VIW27_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HEY5738311.1"
                     /translation="MRALAAEITADPGAVRALRFALGVTIAVALAYGIEWPLSFLLPV
                     LTAVILGLPLPMPSLAAGLRNMLYTLAAFGIGLAFSLFFLRYPLVYLLVLGLVLFHLY
                     YYLNRGGSFWLTLMSMLAILMLPMLANTHEGLASGLALGFVGTSWLTMLMIWLAHLLV
                     PDPAAARMPKPPGFQAGYSKPAAQLALKSTLVIWPLASLFIVFDLMDYLLVMIFSALF
                     ILKPELSKGKEAGLNSLVSTVLGGVCAWFFYWMIVAVPEFHFYIVLTLFTALVFGINI
                     FSAKPSARYFGSAFVALLILVNGSMGEGAEFTQLFAQRIALIVLAVLYIVAVLKVLDN
                     YWPAKVRDPE"
     gene            complement(3676..4770)
                     /locus_tag="VIW27_01235"
     CDS             complement(3676..4770)
                     /locus_tag="VIW27_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016401577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY5738312.1"
                     /translation="MSEETQTIAQTGTDPVRKISKYILYAAIFLFVWYVLADRLAPWT
                     DQARLQTYVIPIVAQVPGRVVEVSVDQDQVVRPGDVLFRIDPTDYELAVETAESALEL
                     TGQEVGAGTASVTSAQAGVVLAETRLEHAKTQSARVFELEKQQVLSRAEGDKARAAVK
                     AAEAEVDNARAGLEQAKQALGKVGEDNPRLRSALASLRKAQLDLSRTTILAPSLGGIT
                     NLRIDVGHYASAGVPVMTFIEADNVWIQADFRENSIANIDAGDPVDIALDVAPGRIFS
                     GTVSSVGFAVDSSASSDAGELAKVGGKSGWLRDAQRFPVIIRFDDDSSRGLRRLGGQA
                     DVLVYSGGNWIVNALGWIWIRLVSLFSFIY"
     gene            complement(4863..>5342)
                     /locus_tag="VIW27_01240"
     CDS             complement(4863..>5342)
                     /locus_tag="VIW27_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5738313.1"
                     /translation="TDLSKATIRSVAQGVLGVAIIQAALSAIGMFAMDVPAWGLWSVI
                     ILVLAIAQLPPILVLGFVIAYVFSVADTTPAIIFTVYAIVVSASDGFLKPLFLGRGMS
                     TPMLVILFGAIGGMMMSGIIGLFVGAIVLALGYELFIKWLDEDGADEEEAATAQASS"
BASE COUNT         1119 a         1703 c         1632 g          888 t
ORIGIN      
        1 gcgatcattc gcttcgtcgg cctggaggcg acgatcaccg atgtattcgc acgctatcgc
       61 tggctcgacg gcagcgaaac cacggcgatc gcgcggccga gccagccgtg gatcgaggtc
      121 gccacgcaac gcggcgcatg gcaggtcgcc ggcgattaca cgctgctcgg catcgaccat
      181 attttgtccg gcttcgacca cctggcgttc gtgcttgcgc tgctgctgat cgtcagcggc
      241 gcgcgccggt tgctgatcac ggtgacctca tttaccgtcg cgcacagcat tacgctggcc
      301 gcggcgacgc ttggcgtact gtgggtaccg ggcccgccga tcgaggcggt gatcgcgctg
      361 tctatcctgt ttctcgccgg cgaactggtc aagatcaacc gcggcgaggc cagccttacc
      421 gcgcgcaatc cgtggatcgt cgccttcgcc ttcggcctgc tgcacggctt cggctttgcc
      481 ggcgcgctcg gcgagatcgg cctgccgcaa aacgagatcg cgctggccct gctgatgttc
      541 aacgtcggcg tcgaactcgg tcagctgctg ttcatcgccg cggcgctggc ggtcatctgg
      601 ctgctgcagc gattgcggct gcaatggccg gactgggcgc aacagctacc cgcctacggc
      661 atcggcggcc tcgccgcctt ctgggtcatc gagcgcgtgg ccggattctg agtccgtccg
      721 ctgtcaaacc ctgcccggcc gagtgtcgaa atcagttgcc gttaccgtcg gtttccttga
      781 aataaagcca gaccagtaag ccaatgaaaa cgagaatctc cacgttcatc acggtatcca
      841 gcagcgcggc gctcacgaca ctccctccgg ctcgtgcttc agggttttca gcagacacac
      901 caccagcacc agcaccacga acacgaacgg cagcgagccg agcgcgatga tcgacttgat
      961 cgcagcgatg tcgccggaga ggatcatcac gtagccgagc gcgccgagaa tgacccccca
     1021 ggcgagcttt atcctcgcgc tcggatccag ttcgccattg ctggagaaca tgccgagcac
     1081 gaaagccgcg ctgaccacgc tggtaacgat gaacaggaag gcggcgacga tgaccaggga
     1141 aatggtcagc aacggcagcg gaaagctctc cagcatgaaa aaggtcaccg cactgacatt
     1201 gttctcgacc acgtccatga tcgccgagcc gcgccgcaat acctcgtaga acccggcgcc
     1261 gccgaaaatg ccgaaccaca gcagcgagaa cacggtcggc ccgaggatca cgccgacgat
     1321 gaattcgcga atcgtgcgcc cgcgcgaaat gcgcgcgatg aacacgccga cgaacggcgc
     1381 ccaggcgatc caccagacca tgtaggtcag cgtccaggat tgaaaccagt tgccgacctg
     1441 ctcgtccatg aaggtgaagg ttctgaaacc gtgcggcaac acgttgctga tgtattcgcc
     1501 gatggcctgg gtgataccac ccatcaggta atgcgtcggc ccggccagca gaataaagac
     1561 catcaggccg ccggcaatcg ccatcgcggt gttcgacagg cgcgccatgc cgctgccgag
     1621 atcgacgacc agcggcggga agtaggcgat acagagcacg acgaataccg ccgtggtcat
     1681 gccgaggccg gtatcgcgca agtcgaacag cgtatcgaga ccatccttga cctggaacac
     1741 gcccatcgcg atcgagccgc cgacaccgat ggcgatggcg acgatcgcca gcaggttggc
     1801 gagccatgag aagctgccgg tgaagcgatt gcggccgaag gtatcgacga tcggcgaacc
     1861 gatcaggctc gggcaaccgc ggcgaaagcc gaaataggcg atcaccaggc cggtcagcgc
     1921 gtagatcgcc caggcatgca ggccccagtg aaaattggtg acgaacaacg ccatgtccgc
     1981 cgcccgcccg ggcgaatggt tttgccgaat cagctcgaag tgggtcagcg gctcggcgct
     2041 gccccagaac agcaggccga cgcccatgcc ggcggcgaac agcatcgtca gccaggatac
     2101 ggtcgagaac tcgggctcgt catcgtcgcg gccgagcttt attccgccat agcgcgacag
     2161 cgcgagccag atactgacca gcagcatgaa ggataccgcc agcatcacga accagccgcg
     2221 gctgacgaac tggatatgca ccagcttcgc cgcgatcgcc gccagaccgg cggtatcgag
     2281 caggccccag atcgcaatcg caccggtcat cagcagcgcg acgatcagca gcgggctgcc
     2341 aaacccggat ttactgttgc cctgctccac actgtcagtc aatgttgtga tcctgttgtc
     2401 gatttacctg cagacgataa ccctatcaca tctttttcca ggcgcttaac ttcgccactg
     2461 tgccatcgcc gcgagaccct ggccgccggc aggcctgcca gcgcaagatc gatttgcgat
     2521 agcaccccgc ccggcatatc aagcctcgca accatcgccc tggatacgca tgcgatccgc
     2581 gtgaagaacg aatattcggc atcgatgatg cgcgtcgtgc tattcggggt cccgcacttt
     2641 cgcgggccaa tagttgtcga gtactttcag cacggcgacg atgtagagta ccgcaagcac
     2701 gatcagtgcg attcgctgtg cgaacaattg ggtgaattcg gcgccctcgc ccatgctgcc
     2761 gttgaccagg atcagcagcg cgacgaaggc ggatccgaaa tacctggcgc tcggtttcgc
     2821 ggagaagatg ttgattccga ataccagggc ggtgaacaag gtcagcacga tgtagaagtg
     2881 gaattccggc accgcgacga tcatccagta gaaaaaccag gcgcagacac cgcccagcac
     2941 ggtcgacacc agcgagttca gaccggcctc cttgcccttg ctgagctcgg gtttgagaat
     3001 gaagagcgcc gagaaaatca tcaccagcag gtaatccatc aggtcgaaga cgatgaacag
     3061 gctggcgagc ggccagatga cgagcgtgct tttcagcgcc agctgcgccg cgggtttcga
     3121 atagcccgcc tgaaatccgg gcggcttcgg catcctcgcc gcggccgggt cgggaaccag
     3181 caagtgggcg agccagatca tcagcatcgt cagccagctg gtgccgacga agcctagcgc
     3241 aaggcccgag gcaagtccct cgtgagtatt ggccagcatc ggcaacatca atatcgcgag
     3301 catgctcatc agggtcagcc agaaggaccc gccgcgattg agatagtaat agagatgaaa
     3361 cagcaccagt ccgagtacga gcagatagac cagcgggtag cgcaggaaaa acagcgaaaa
     3421 cgcgaggccg atgccgaagg ctgccagggt gtacagcatg ttgcgcaggc cggcggccag
     3481 cgatggcatt ggcagcggca gcccgaggat gaccgcggtc agcaccggca gcaggaacga
     3541 cagcggccac tcgatgccgt aggcgagtgc caccgcgatg gtcacgccga gcgcgaagcg
     3601 cagcgcgcgg acggcgcccg ggtcggcagt gatttcagcg gccagggcgc gcatggaaac
     3661 cgtccgcacg acgacctagt aaatgaacga aaacaggctc accaggcgga tccagatcca
     3721 gccgagcgcg ttgacgatcc agttgccgcc gctgtagacc agcacgtcgg cctgcccgcc
     3781 gaggcggcgc agcccgcgcg agctatcgtc gtcgaacctg atgatgaccg gaaagcgctg
     3841 tgcatcccgc agccagccgg acttgccgcc gaccttggcc agttcgccgg cgtcgctgct
     3901 cgccgaactg tcgaccgcga aaccgacgct gctgacggtg ccgctgaaga ttcgcccggg
     3961 cgctacgtcg agggcgatat cgaccggatc gccggcgtcg atgttggcga tactgttttc
     4021 gcggaaatcg gcctgtatcc agacattgtc cgcctcgatg aaggtcatca ccggcacgcc
     4081 cgcgctggcg tagtgaccga cgtcgatgcg caggttggta atgccgccga gcgacggcgc
     4141 gagaatggtc gtacggctca ggtcgagctg cgccttgcgc aggctggcga gcgccgagcg
     4201 caaccgcgga ttgtcttcgc cgaccttgcc gagggcctgc ttggcctgtt cgaggccagc
     4261 gcgcgcatta tcgacctcgg cttccgccgc cttgaccgcg gcacgtgcct tgtcgccttc
     4321 ggcgcgtgac agcacctgct gcttttccag ttcgaagacg cgcgcgctct gcgttttggc
     4381 atgctcgagg cgagtctcgg ccagcaccac gccggcctgc gcgctggtca ccgaggcggt
     4441 gccggcacca acttcctgac cggtcagttc gagcgcggat tcggcggtct cgaccgcgag
     4501 ttcgtaatcg gtcgggtcga tgcgaaacag cacatcgcca ggccgcacga cctggtcctg
     4561 gtcgacgctg acctcgacca cgcggcccgg cacctgtgcg acgatcggaa tgacataggt
     4621 ttgtaatcgc gcctggtccg tccacggggc caggcggtcg gcgaggacgt accagacgaa
     4681 caggaagata gcggcgtaga gaatatattt cgagattttg cgcaccgggt ccgtgcccgt
     4741 ttgcgctatg gtctgggttt cttccgacat agtttattcc gcgttgaata tctgtgatta
     4801 caaccgttcg tgatcggctt cgaatcgcgc aaaaagtaga acagcatttt tcgcacaacg
     4861 catcaagatg atgcctgcgc ggtcgcggcc tcttcttcgt cggctccgtc ctcgtcgagc
     4921 cacttgataa acagctcgta gcccagggcc agcacgatcg caccgacgaa cagaccgata
     4981 atgcctgaca tcatcatgcc gccaatcgcg ccaaacagta tcaccagcat cggcgtactc
     5041 atgccgcgcc cgagaaacag cggcttgaga aagccgtcgc tggcgctgac gacgatcgcg
     5101 taaacggtaa aaataatcgc cggggtggta tccgccaccg aaaacacata ggcgataacg
     5161 aaacccagca ccaggatcgg cggcaattgc gcgatcgcca gcaccaggat gatcaccgac
     5221 catagccccc aggccggcac atccatcgcg aacatgccga tagccgaaag cgcggcctgt
     5281 ataatcgcga cgccgagcac cccctgggcc acgctacgaa tggtcgcctt ggacaaatcg
     5341 gt
//
LOCUS       DATCZV010000052         6422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_533408, whole genome shotgun sequence.
ACCESSION   DATCZV010000052 DATCZV010000000
VERSION     DATCZV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533408"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VIW27_01245"
     CDS             <1..897
                     /locus_tag="VIW27_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5738314.1"
                     /translation="DALYLQPRVASIYLLRDGDQAALVDTGTCHSLDNVLATLRTLEI
                     APSQVRYVIPTHVHLDHAGGASAMMRAFDQATLLIHPRGARHMADPQKLIDGTVAVYG
                     QQKFDRLYGKIEPIDERRMHIVEDDERISLNRRELLFFDTPGHARHHFCIYDAASEGV
                     FTGDTFGLSYDAMKDLPRGLLPTTPPSQFDPPALRESIARIAALQPRRLYLTHFGAFT
                     DPLAQVDSFHRWLDAYVDLCERLQPADQTAESELEAELTALVIDGLAPGAAREAVAGS
                     LRMDLRLNAQGLAHWWRSTHRD"
     gene            890..1348
                     /locus_tag="VIW27_01250"
     CDS             890..1348
                     /locus_tag="VIW27_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5738315.1"
                     /translation="MTEAVWKPHATVAALCERDGKFLLVREKADGLVVYNQPAGHLDP
                     GESLLDAVVRETLEETRYAFEPQALLGIYRYRPHAERSDTYLRFLFRGEVGERLDGPL
                     DDDIIAAEWLSYEQVLDCREQHRSPMVMRGIDDFRQHPGYPLDIISAEFA"
     gene            1345..1929
                     /locus_tag="VIW27_01255"
     CDS             1345..1929
                     /locus_tag="VIW27_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738316.1"
                     /translation="MIRLATAVLMLAAWLPAAAMPYFVHCYDFSCKTTLELRYSEAQW
                     QRIRSIMEPGQADADAEKQAIRQAVAMMERFSGELAGTHLDKAGNYPGYDLPGQMDCI
                     DESTNTFQYLAALDQLGLMKWHRVAPKQRRIVWFISHWTATIAETGSGRRFAVDSWYR
                     DNGEPPYIQPLDDWQRKRDFPPRYNPEFAALGEP"
     gene            1932..3029
                     /gene="mnmA"
                     /locus_tag="VIW27_01260"
     CDS             1932..3029
                     /gene="mnmA"
                     /locus_tag="VIW27_01260"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY5738317.1"
                     /translation="MVSGGEKIIVGMSGGVDSSVSAWLLQQQGYDVEGLFMKNWEEDD
                     SEEYCSAAVDLADAQQVCERLGIRLHTVNFSSEYWDNVFEYFLHEYRIGRTPNPDVMC
                     NREIKFKAFLDHAQRLGARRIATGHYVRVRRRGEYFELLRGLDANKDQSYFLYALDQH
                     QIGHALFPVGELDKSAVRDIAGQQQFAVAAKKDSTGICFIGERKFRDFLERFIPAQPG
                     DIVSLDGSLLGSHSGLMYYTHGQRQGLGIGGTESGSDDPWYVTGKDLEHNRLIVVQGH
                     DHPALFHTHCRVSDLHWIARPIDAPGSECSAKIRYRQRDSACRLQSLDGNTAVIEFAA
                     PQRAITPGQALVFYDGEQCLGGGVIDTAYNS"
     gene            3026..3661
                     /gene="hflD"
                     /locus_tag="VIW27_01265"
     CDS             3026..3661
                     /gene="hflD"
                     /locus_tag="VIW27_01265"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEY5738318.1"
                     /translation="MNRFENQVLALAGMFQAASLIDELAFGGNLDDAAFEASLDSLFT
                     FEAENPRQVFGGVDKLELGFRALETYLGGDLAGGSRNIAYYVLSMMKIALRLLRDDEL
                     SRAMLARLHDIQRNARDFDMSRSALVIKIDGLYQETISNLRPRIMVAGEQNYLTNTDT
                     AARVRALLLAGIRAAVLWRQLGGSKWKLLFSRKRYAAVAHYLAESCQPPAC"
     gene            complement(3658..4146)
                     /locus_tag="VIW27_01270"
     CDS             complement(3658..4146)
                     /locus_tag="VIW27_01270"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY5738319.1"
                     /translation="MSDIKPVRVLFVCMGNICRSPTAHGVFQALVAAEGLEASIGVDS
                     AGTHGYHTGSPPDPRSQATARAHGVDLSALRARRFESADFRDFDYLLAMDRGNLADML
                     ALRPGRDDTAPELMLAYSSRFREQEIPDPYYGNDGFELVFEMIDDASRGLLRQIREHH
                     QI"
     gene            4247..5467
                     /locus_tag="VIW27_01275"
     CDS             4247..5467
                     /locus_tag="VIW27_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY5738320.1"
                     /translation="MLIIPEIQIVEGKVITRAAVEGDDIYHEISPRDAVNKFIDDGAE
                     MLQLVDVDAARSKATNNQQLIKGIIAETDIPIQVAGGIRTLSQINDWFEAGAARVVLG
                     TVAITESQLVVEAANRHPGGIIVHLATRGGYVMIDGWKTQTAFMPGDLIRDLQMTGIA
                     GVVHKGTERLDTDVEEALALTEKLSHDVSIPVYSSGTVRTLDDLARLRYLPNINGAII
                     SHALLSGAISLKDALQVAAEKETNLEPESITPNVNMGIHHGVRAYLAAYNSSQAARVW
                     NNRLRDTLTEDNPYMEVLIPQLDLQIDYERMSPRDIQAAYESELGRADVVIVVLEGVE
                     SEAWTGFECGYARAKGKYIYGITSEGELKGASERRFEAMCDELISYTASNDITKAHAE
                     ISHALATSVMTKNQ"
     gene            complement(5813..6133)
                     /locus_tag="VIW27_01280"
     CDS             complement(5813..6133)
                     /locus_tag="VIW27_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEY5738321.1"
                     /translation="MKNFLTLVAATLLLGGCSQLKLTDDGEKVRVLEPSEVGSCRLLG
                     KTNASVTSRVIVERPEEAVSKELRIVARNSAARMGGDTIVPLTIVEGGQQTFQVYKCV
                     NPDG"
     gene            complement(6162..>6422)
                     /locus_tag="VIW27_01285"
     CDS             complement(6162..>6422)
                     /locus_tag="VIW27_01285"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873024.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY5738322.1"
                     /translation="VMLKLTLPEQDNFYASCIEHPRVLKVVALSGGYSREEANQRLAR
                     QNGMVASFSRALTEGLSAQQSEDEFNQMLDASIESIFRASCT"
BASE COUNT         1251 a         2054 c         1951 g         1166 t
ORIGIN      
        1 gacgcgctgt acctgcagcc gcgcgtggct tcgatctacc tgttgcgcga cggcgaccag
       61 gcggcgctgg tcgacaccgg cacctgtcac tcgctggaca acgtgctggc gacactgcgg
      121 acgctggaaa tcgccccctc gcaggtcagg tacgtgattc cgacccacgt acacctcgac
      181 cacgccggcg gcgcgagcgc gatgatgcgc gcctttgatc aggccaccct gttgattcac
      241 ccgcgaggcg cccgccacat ggccgatccg cagaagctca tcgacggtac cgtcgccgtc
      301 tacggccagc agaaattcga ccggctgtac ggtaaaatcg agcccatcga cgagcggcgc
      361 atgcatatcg tcgaggatga cgagcgcatt tcgctgaatc gacgcgaact gctgttcttc
      421 gatacccccg ggcacgcacg ccatcatttc tgcatctacg atgcggcgag cgagggcgtg
      481 ttcaccggcg acaccttcgg gctcagctac gatgcgatga aggacctgcc gcggggcctg
      541 ctgccgacca cgccgccatc gcagttcgat cccccggcgc tgcgggaatc gatcgcgcgc
      601 atcgccgcac tgcagccgcg acggttgtac ctgacgcatt tcggcgcctt taccgatccg
      661 ctggcgcagg tcgacagttt tcaccgctgg ctcgatgcct acgtcgacct ctgcgaacgt
      721 ctgcaacccg ccgatcagac tgccgagagc gaactcgagg cggaactgac ggcgctggtg
      781 atcgacgggc tggcgcccgg cgcggcgcgc gaagcggtcg ccggcagcct gaggatggac
      841 ctgcgcctca atgcacaggg cctcgcccac tggtggagaa gcacgcatcg tgactgaagc
      901 ggtgtggaaa ccccatgcga cggtcgcggc actatgcgag cgcgacggca aattcctgct
      961 ggtgcgcgaa aaggccgacg gcctggtggt ctacaaccag ccagcgggac acctcgaccc
     1021 cggcgagagc ctgctcgacg cggtggtccg cgaaactctc gaggaaaccc gctatgcctt
     1081 cgaaccccag gcgctgctgg gtatataccg ttaccggccg catgccgaac gcagcgatac
     1141 ctacctgcgt ttcctgtttc gcggcgaggt cggagaacgg ctcgacgggc cgctcgacga
     1201 cgatattatc gccgccgaat ggcttagcta cgagcaggtg ctcgattgtc gcgagcagca
     1261 tcgcagcccg atggtgatgc gcggcatcga tgatttccgc cagcacccgg gttacccgct
     1321 agatatcatc agcgcggagt tcgcgtgatt cgcctggcca cggccgtgtt gatgctcgcc
     1381 gcctggctgc cggccgccgc gatgccctac ttcgtgcact gttacgattt cagctgcaag
     1441 accaccctcg agctgcgcta cagcgaagcc cagtggcagc gaattcgttc gatcatggaa
     1501 ccgggccagg ccgacgccga cgcggaaaag caggcgattc gccaggcggt ggcgatgatg
     1561 gagcgcttca gcggcgaact cgccggcacc cacctcgaca aggcaggcaa ctaccccggg
     1621 tacgacttgc ccgggcaaat ggattgtatc gacgaatcga ccaatacctt tcagtacctg
     1681 gccgcgctcg accagctcgg cctgatgaaa tggcaccgcg tggctcccaa gcaacgccgc
     1741 atcgtctggt tcatcagcca ctggaccgcg accattgccg agaccggcag cgggcgtcgc
     1801 ttcgcggtcg actcctggta ccgcgacaac ggcgagccgc cttatataca gccgctcgac
     1861 gactggcagc gcaagcgtga cttcccgccg cgttacaacc cggagttcgc ggcgctcggg
     1921 gaaccgtagg catcgtgagc ggcggcgaaa aaatcatcgt cggcatgtcc ggcggagtcg
     1981 actcctcggt ttcggcatgg ttgctgcagc agcagggtta cgatgtcgag ggcttgttca
     2041 tgaagaactg ggaggaggac gacagcgaag agtattgctc ggcggccgtc gatctagccg
     2101 atgcgcagca ggtttgcgag cgcctcggca tacgcctgca cacggtgaat ttttccagcg
     2161 agtactggga taacgtgttc gagtatttcc tgcacgaata tcgcatcggt cgcacgccca
     2221 accccgacgt catgtgtaac cgcgaaatca agttcaaggc ctttctcgat cacgcgcaac
     2281 ggctcggcgc gcggcgtatc gccaccggcc actacgtacg cgtgcgccgt cgtggcgagt
     2341 atttcgaact gctgcgcgga ctcgatgcca acaaggacca atcctacttt ctgtatgctc
     2401 tcgaccagca ccagatcggt catgcgctgt tcccggtcgg cgaactcgac aagtccgcgg
     2461 tgcgcgatat cgctggccaa cagcaattcg cggtggcggc gaaaaaagac agcaccggga
     2521 tctgctttat cggcgagcgc aagtttcgcg actttctcga gcgtttcatt ccggcgcaac
     2581 ccggcgacat cgtatccctg gacggcagcc tgctcggcag ccacagcgga ctgatgtact
     2641 acacccacgg tcagcgccag ggcctcggta ttggcggcac cgagagcggc agcgacgatc
     2701 cctggtacgt caccggcaag gatctcgagc acaaccgcct gatcgtcgtc caggggcacg
     2761 atcaccctgc cctgttccat acgcattgcc gggtctctga cttgcactgg atcgcgcgcc
     2821 caatcgatgc gccaggcagc gaatgcagcg ccaagattcg ttaccggcaa cgcgacagcg
     2881 cctgccgcct gcaatcgctc gacggcaata ccgcggttat cgagttcgcg gcaccgcagc
     2941 gcgcgatcac gccgggacag gcgctggtgt tttacgacgg cgaacaatgc ctcggtggcg
     3001 gcgtcatcga taccgcgtac aactcgtgaa tcgcttcgag aaccaggtac tggcactcgc
     3061 cggcatgttc caggcggcgt cgttgatcga cgaactggcg tttggcggta atctcgacga
     3121 tgcggccttc gaggccagcc tcgacagcct gttcaccttc gaggccgaaa atccgcgcca
     3181 ggttttcggc ggtgtcgaca agcttgaact cggattccgc gcactcgaga cctatctcgg
     3241 tggcgacctc gccggcggca gccggaatat cgcttattac gtgctctcga tgatgaagat
     3301 tgcgctgcgc ctgttacggg acgacgaact gtcgagggcg atgctcgcgc gtctccacga
     3361 tattcagcgc aacgccaggg atttcgatat gtcgcgctcc gcgctggtga tcaagatcga
     3421 cgggctttac caggaaacca tctccaatct gcgtccgcga atcatggttg ccggcgaaca
     3481 gaactacctg accaacaccg ataccgccgc ccgcgtcagg gccctgctac tggccggtat
     3541 tcgcgccgcg gtactctggc gacagctcgg tggcagcaaa tggaaactgc tgttctcgcg
     3601 caagcgctac gccgcggtgg cgcattacct ggccgaatcc tgccagccgc ccgcttgcta
     3661 gatttgatgg tgctcgcgaa tttgtcgcag taggccccgc gacgcgtcgt cgatcatttc
     3721 gaagaccagc tcgaaaccgt cgttaccata gtacggatcc ggaatctcct gctcgcgaaa
     3781 gcgcgatgaa taggccagca tcagttcggg ggctgtgtcg tctcgaccgg gtctgagcgc
     3841 cagcatgtcg gctagattgc cgcggtccat cgccagcagg taatcgaaat cccgaaaatc
     3901 ggcggattcg aaacgccgcg cgcgcagcgc ggagaggtcg acaccgtgag cgcgtgcggt
     3961 ggcttgcgag cgcgggtcgg gcgggctgcc ggtatggtaa ccgtgggtgc cggccgaatc
     4021 gacaccaatc gacgcctcga gtccctcggc tgcgaccagc gcctgaaaaa cgccgtgggc
     4081 ggtgggcgaa cggcatatat tacccatgca gacgaacagc actcggaccg gtttgatgtc
     4141 gctcatagat acgaatatac catcttgaag caacaattgg tgttcgcccg gcctgtttca
     4201 ggcgttacaa tgacggcccg tttcgaactt actccaggtt atcgccatgc tgatcattcc
     4261 cgaaatccag atagtggaag gcaaggtcat cactcgcgcc gcggtcgaag gcgacgatat
     4321 ctaccacgag atttcgccgc gcgacgcggt gaacaagttc atcgacgacg gtgccgaaat
     4381 gctgcagctc gtcgacgtgg acgcggcccg ctccaaagcg accaacaacc agcagctgat
     4441 caagggcatc atcgccgaga ccgatatccc gatccaggtg gccggcggca ttcgcaccct
     4501 gagccagatc aacgactggt tcgaggccgg cgctgcgcgc gtggtgctgg gcaccgttgc
     4561 gatcaccgaa tcgcaactgg tggtcgaggc ggccaatcgt caccccggcg gcatcatcgt
     4621 gcacctggcg acgcgcggtg gctacgtgat gatcgacggc tggaagacgc agaccgcgtt
     4681 catgcccgga gacctgatcc gtgacctgca gatgacgggt atcgccggcg tcgtacacaa
     4741 gggtaccgag cgtctcgaca ccgacgtcga ggaagcgctg gcattgaccg agaagctcag
     4801 ccacgatgta tcgattccgg tttactcgag cggcacggtg cgcacgctgg acgacctcgc
     4861 gcgcctgcgt tacctgccga acatcaatgg tgcgatcatc agccatgcgc tgctaagcgg
     4921 cgcaatttcg ctcaaggacg cgctgcaggt cgccgccgag aaggaaacca acctcgagcc
     4981 cgagtcgatc acgcccaacg tcaacatggg catacaccat ggcgtgcgtg cctacctcgc
     5041 cgcctataac agttcgcagg cggcgcgggt ctggaacaac cgtctgcgcg ataccctgac
     5101 cgaggacaac ccctacatgg aagtcctgat tccgcagctc gacctgcaaa tcgattacga
     5161 gcgaatgtcg ccgcgggata tccaggccgc ctacgagtcc gagcttggcc gtgccgacgt
     5221 cgtcatcgtc gtgctcgagg gcgtcgagtc ggaagcctgg accggtttcg aatgcggcta
     5281 tgcgcgcgcc aagggcaagt acatttacgg tattacctcg gaaggagaac taaaaggcgc
     5341 cagcgagcgg cgtttcgagg cgatgtgcga cgaattgatc agctataccg cgagcaatga
     5401 catcaccaag gcgcatgcgg agatttcgca tgctttggcc accagcgtca tgaccaaaaa
     5461 ccagtagggc tgcggacgcc gcaaagcgca ttgtgtgagt tgtactcgcc agcgcccgct
     5521 ctcaacgtag cttgagctac gcctcgcaga cgcgggctcc gtgcaacccc tcgcaacgtc
     5581 tttgcgacgc cctcgcgaat tttcgggaaa tgcagaccga cggctgcgga cgccgcaaag
     5641 cgcggtgggt gagccgtact cgccagcacc cgctctcaac gtagcttgag ctacgcctcg
     5701 cagacgcggg ctccgtgcaa cccctcacac cgtctttgcg acgcgccggg tgaatcctct
     5761 gcgaacacca gccgatagag gactggcggc tgtcgacaac cgcgccgggc gcttagccgt
     5821 caggattgac gcacttgtag acctgaaagg tctgctggcc tccctcgacg atggtcagcg
     5881 gtacgatggt atcgccgccc atgcgtgcgg cgctgttacg cgcgacgata cgcagttcct
     5941 tggataccgc ttcctccggc cgttcgacga tcacccggga ggtgaccgac gcattggtct
     6001 tgccgagcag ccggcaacta ccgacctcgc tgggttcgag cacgcggact ttttcgccgt
     6061 cgtcggtgag cttcaattgt gaacagccgc ccagcaacag ggtcgcggct acgagcgtca
     6121 ggaaattctt catattcgat gtcttcctcg attatcggct atcaggtaca ggaggcgcgg
     6181 aagatacttt ctatcgacgc gtcgagcatc tggttgaact catcctcgct ttgctgggcg
     6241 ctgaggccct cggtgagcgc gcgtgaaaag ctcgcgacca taccgttttg tcgcgccagc
     6301 cgctggttgg cctcttcccg cgaatatccg ccggacagcg ccaccacctt cagcacccgc
     6361 gggtgctcga tgcaactggc atagaaatta tcctgctcgg gcagagtcag cttgagcatc
     6421 ac
//
LOCUS       DATCZV010000053         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_586293, whole genome shotgun sequence.
ACCESSION   DATCZV010000053 DATCZV010000000
VERSION     DATCZV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586293"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VIW27_01290"
     CDS             <1..888
                     /locus_tag="VIW27_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5738323.1"
                     /translation="VICAGGCLGILIPPSIMLIVYSAIAELSPLRLYAAALFPGMMLA
                     GMYMVYVVGRAALNPSLAPKPKEEDVPPFGRILWELLTSFVPLAVLIMSVLGAILMGL
                     ATPAEAAGVGAFGGLILAVIYRSLTWERLKQAVYLTAKTSAMVCWLFVGSWTFAAVFS
                     YLGGHDLVAEWVLGMELGTVGFLVLAQTIIFLLGWPLEWTEIIIIFVPIFLPMLETFN
                     VDPLFFGILVALNLQTSFLTPPMAMAAYYLKGVAPPYIQLIDIFKGIMPYLAIIILSM
                     VLLYNFPGIALWLPVYLFG"
     gene            912..>2176
                     /locus_tag="VIW27_01295"
     CDS             912..>2176
                     /locus_tag="VIW27_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5738324.1"
                     /translation="MDRIDPDAANLLGARAARAAIDSGLLSSEQLVEACFARIDLLED
                     EIGAWAHIDRETALAQARAADAFRMRGLPVGALHGLPIGIKDIIDTADYPTERGTVLH
                     HGRRPDRDATLVSLLKEAGAIILGKTVSTEMAVYAPGKTRNPHNLEHTPGGSSSGSAA
                     AVAAAMAPLAVGTQTNGSVIRPASYCGVYGFKPSFGRISRHGVLEQSPPLDTVGVFAR
                     DLDDLAMIADVLMRFDAQDAAMSPVAPPRLADVLAEDVPLGLHFVFVRTPAWEQVEPQ
                     TKDALREFIDAVNDTREKTIDVIDLPPPFAELYEDHRKVMEGDLARSFADEYRRGKSE
                     LSDVLREMIERGQQVSDAEYDAALARRTDYNALLDEFFDEYDAILTPATPGPAPAGVE
                     ATGSPIMSTIWTFCGTPALSLPVFRSTQGL"
BASE COUNT          355 a          715 c          682 g          424 t
ORIGIN      
        1 gtcatctgcg ccggcggctg cctcggcata ttgattccgc cgtcgatcat gctcatcgtc
       61 tactcggcga tcgccgagct gtcgccgttg cgcctgtatg ccgcggcgct gttcccgggc
      121 atgatgctgg cgggcatgta catggtttac gtggtcggcc gcgcagcgct caatccgagc
      181 ctcgcaccca aacccaagga ggaggacgtg ccgccttttg gcaggatcct gtgggagtta
      241 ctgacctcct tcgtgcccct cgcggtgctg atcatgagcg tgctcggcgc gatcctgatg
      301 ggacttgcga ccccggccga ggccgccggc gtcggcgctt tcggtggact gatactggcg
      361 gtgatttacc gctcgcttac ctgggagcgt ctcaagcagg cggtttacct gaccgccaag
      421 acctcggcca tggtgtgctg gctgttcgtc ggttcatgga ccttcgccgc ggttttttcc
      481 tatctcggtg gtcacgacct ggttgccgaa tgggtgctcg gcatggagct gggcacggtc
      541 ggtttcctgg tactggcgca aaccatcatt ttcttgctcg gctggccgct ggagtggacc
      601 gagatcatca tcatcttcgt gccgatcttc ctgccgatgc tggaaacctt caacgtcgat
      661 ccgctgttct tcggtatcct ggtagcgctg aacctgcaaa cttcgttcct gacgccgccg
      721 atggcgatgg ccgcctatta cctcaagggc gtggcgccgc cgtatatcca gctgatcgat
      781 atattcaaag gcatcatgcc ttatctggcg atcatcatcc tgtcgatggt attgctgtat
      841 aacttcccgg gtatcgcgct atggctgccg gtctacctgt tcggataggc gctgcggttc
      901 aggtgtaatc aatggacagg atcgacccgg atgccgccaa cctgctcggc gcgcgcgcag
      961 cgcgcgccgc catcgacagc ggcctgctga gttccgagca actcgtcgag gcctgtttcg
     1021 cgcgtatcga cctgctcgaa gacgagatcg gcgcctgggc gcatatcgat cgagaaaccg
     1081 cgctcgcgca ggcgcgcgcg gccgacgcgt ttcgcatgcg cgggctgccg gtcggcgcgc
     1141 tgcacggcct gccgatcggt atcaaggaca ttatcgatac cgccgactac ccgaccgagc
     1201 gcggtaccgt actgcaccat gggcgccggc cggatcgcga cgcgaccctg gtttcactgc
     1261 tgaaagaagc cggcgcgatc atcctcggca agaccgtcag caccgagatg gcggtctacg
     1321 cgcccggtaa gacccgtaac ccgcacaacc tcgaacatac gcccggcggc tcgtcgagcg
     1381 gctcggcggc ggcggtcgcc gcggcgatgg cgccactcgc agtcggcacc cagaccaacg
     1441 gctcggtgat tcgtcccgcc tcgtattgcg gcgtctatgg cttcaagccg agcttcgggc
     1501 gcatctcacg ccacggtgtg ctcgagcagt cgccgccgct ggacaccgtc ggcgtgttcg
     1561 cgcgcgatct cgacgacctg gcgatgatcg ccgacgtgct aatgcgcttc gatgcccagg
     1621 atgcggcaat gtcgccagtc gcgccgccgc gccttgccga cgtgctcgcc gaggatgtgc
     1681 cgctggggct gcatttcgtc ttcgtgcgca cgcccgcctg ggagcaggtc gagccgcaga
     1741 ccaaggatgc actgcgcgaa ttcatcgacg cggtcaacga cacccgcgaa aaaaccatcg
     1801 acgtgatcga tttgccgccg ccgttcgcgg aactgtacga agaccaccgc aaggtcatgg
     1861 aaggcgacct ggcacgcagc ttcgcggacg agtaccggcg cggcaagtcc gagcttagcg
     1921 acgtcctgcg cgagatgatc gagcgcggcc agcaggtcag cgatgccgaa tacgacgcgg
     1981 cactcgcccg gcgcacggat tacaacgcgc tgctcgatga atttttcgac gaatacgatg
     2041 ccatcctgac gccggcgaca ccgggcccgg caccggccgg cgtcgaggcc accggcagcc
     2101 cgatcatgag taccatctgg actttttgcg gcacaccggc actcagcctg ccggtgtttc
     2161 ggagcaccca gggcct
//
LOCUS       DATCZV010000054         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_692454, whole genome shotgun sequence.
ACCESSION   DATCZV010000054 DATCZV010000000
VERSION     DATCZV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692454"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VIW27_01300"
     CDS             complement(<1..1352)
                     /locus_tag="VIW27_01300"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5738325.1"
                     /translation="MNTVSRRNRNTRRARLQAARELPANKAPEIIDNGFRPVFGFYPD
                     NQIDALRDRSLRLLETHGVSIEHGAAGKLLTAAGASRDGLRYRLPRRLVEEALEATPK
                     TVRLGARDPAWDLQLPRADGGFVMRTVTGAHGFIDAESGDYRDLQLEDIDIIAHLGNH
                     LDQVGFIGHPFVNGVPPPTADIHGFARLVARTQKHAWIQPYEFDNVDYLMRIAMTAAS
                     GDATFRERPTASCIVTAFSPLEFKPMDVEAIRLAALYGMPVHACSLPSAAGTAPITMP
                     GVAVMAAAEILAMVVLAHLFGPGLPVIATPLLFSLDVRTGCCLQSSVEVLQGTSIAVQ
                     LMKQGFGLVTHTYGTGSDTPAPGPQAQAESALRSQLVSLSGADILGGVGQLGCATIFS
                     PVQAILDNELGAMMNHYLRAFPVDDESTGWEVLRNVEPGGNFLAEEHTVRHCRGMFMP
                     "
     gene            complement(1612..2952)
                     /locus_tag="VIW27_01305"
     CDS             complement(1612..2952)
                     /locus_tag="VIW27_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5738326.1"
                     /translation="MNAAVETFLSDEIGPLPFCPGCGHDRLVKALDKALVSLQPEPNK
                     VVIVTDIGCIGLADRYFVTHAFHGLHGRSITYACGLKLARPELTVIVLKGDGGCGIGG
                     AHLLNVARRNIGITLIVANNFNYGMTGGQHSVTTPLEGLTATTPQGNIEGALDLCGVT
                     IAAGAPWVYRGTGFDGELPDRIADAIRQPGFAMLDVWDMCTAYYLPRNRFGKKDLAAT
                     MERLGLQSGLLADRPRPEFGELYRQIHADAPPAPTAKPRIEAKYKHALARQTGIVIAG
                     SAGQKVRSAAGLLARAGMFAGLEATQKDDYPITVMTGHSLSEIILSPERIDYTAIESP
                     DYLLLISRDGLDKARGLLQRLDRSATVYAEETLELPVTAAKVVRLPFAATAKKIDRLS
                     IGTLALAAMLHDGGLLPLQALEESISAVLKPALAEVNRRALAAGAALAGEAREA"
     gene            complement(2949..>3421)
                     /locus_tag="VIW27_01310"
     CDS             complement(2949..>3421)
                     /locus_tag="VIW27_01310"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate flavodoxin/ferredoxin oxidoreductase"
                     /protein_id="HEY5738327.1"
                     /translation="GGEQIARFTTSTHDKDGYLAASPPVIQEMIDHYAAKVDRAADEI
                     ALVKQDFQPGAEILVVSYGITSRSAAVAVRSARAAGVKISSLVLQTLYPVPATALVSA
                     MRGIKRIVVPEMNMGQYILEIERLAPSGVEIIGVNKMDTTLVSPQEILEQGGLK"
BASE COUNT          579 a         1120 c         1100 g          622 t
ORIGIN      
        1 ctcggcatga acatgccgcg gcagtgcctg acggtatgtt cctcggcgag gaaattcccg
       61 ccgggctcga cattccgcag cacttcccag ccagtgcttt cgtcgtcgac tggaaaggcc
      121 cgcaggtaat gattcatcat cgccccgagc tcgttgtcga gaatcgcctg cacggggctg
      181 aatatcgtcg cgcagcccag ttgcccgacc ccgccgagaa tatcggcccc gctcagcgac
      241 accagctgcg agcgcagtgc cgactcggcc tgcgcctgcg ggccgggggc gggggtatcc
      301 gatcccgtgc cataggtgtg cgtgaccagc ccgaatccct gcttcatcag ctgcaccgcg
      361 atactcgtac cctgcagcac ctcgacactg gattgcaggc agcacccggt gcgcacgtcc
      421 agcgaaaaca gcaatggcgt ggcgataacc ggcagcccgg ggccgaacag gtgggccagc
      481 accaccatcg cgaggatctc cgccgccgcc atcaccgcca ccccgggcat ggtgatcggc
      541 gcggtacccg ccgcgctcgg cagactgcag gcatgaacgg gcattccgta gagcgccgcc
      601 aggcgaatcg cttcgacgtc catgggcttg aattcgagcg gcgaaaaggc ggtgacgata
      661 caactggcgg taggccgctc gcgaaacgtc gcgtcgccgc tggcggcggt catcgcgatc
      721 cgcatcaggt aatccacgtt gtcgaattcg tagggctgaa tccaggcatg cttttgcgtg
      781 cgtgcgacga ggcgcgcgaa accgtggata tcggcggtcg gcggcggtac gccgttgacg
      841 aacggatggc cgatgaaacc gacctggtcg aggtggttgc cgagatgcgc aatgatgtcg
      901 atatcctcga gctgcaggtc gcggtaatcg ccgctctcgg catcgatgaa accatgcgcg
      961 ccggtgacgg tgcgcatcac aaagccgcca tcggcacgcg gcaactgcag gtcccacgcc
     1021 gggtccctgg cgcctagcct taccgtcttc ggggtcgcct ccagggcttc ctcgaccagc
     1081 cgccggggaa gcctgtagcg caggccgtcg cgcgacgcgc cggcggcggt cagcagcttg
     1141 cccgccgcgc cgtgctcgat gctcaccccg tgggtttcca gcaggcgcag gctgcggtcg
     1201 cgcagcgcgt cgatctggtt gtcggggtaa aagccgaaga cgggcctgaa gccgttgtcg
     1261 atgatttccg gcgccttgtt ggccggcagt tcgcgggcgg cctgcagccg cgcccggcgg
     1321 gtatttctgt ttctcctgct gactgtattc atgttgaaac cctcaagcgt tgattgtcct
     1381 ggcacacacc gtttagcgct cactgcctgc cgacttgagt aatgcgatct gtcgcgacgc
     1441 gaatgcaccc gcatggcacg ccgccgactt ctccaacatg catggatcga ttagtgtttt
     1501 ctgccgataa cgtctcccga aaaaatgaat attatcggcc gggtcggtgc cgggccgttg
     1561 acgccagtca gttagcgcgc attttcccgg cgcacacccc ggccagcgca attacgcttc
     1621 gcgcgcctcg ccagccagcg cggcgcccgc tgccagggcc cgccgattca cctcggcgag
     1681 cgccggcttg agcaccgccg atatggattc ctcgagcgcc tgcagcggca acaggccgcc
     1741 gtcgtgcagc atcgccgcca gtgccagcgt gccgatcgac aggcggtcga tctttttcgc
     1801 cgtggcggcg aacggaagcc gaacgacttt cgccgcggtc acgggaagtt cgagggtttc
     1861 ctcggcgtag acggtggcgc tacggtcgag cctttgcagc aggcctctgg ctttgtccag
     1921 cccgtcccgc gaaatcagca gcaggtaatc gggggattcg atcgcggtgt aatcgatgcg
     1981 ctcggggctg agaatgattt cgctgagcga gtggccggtc atcaccgtga tcgggtaatc
     2041 gtctttttgc gtcgcctcga ggcccgcgaa catcccggcg cgggccagca acccggcagc
     2101 ggagcgtacc ttctggccgg cactgccggc gatgacgatg ccggtttgcc gcgccagtgc
     2161 atgcttatac ttcgcctcga tgcgtggttt cgccgtgggc gccggcggcg cgtcggcgtg
     2221 gatttgacga tagagttcgc caaactccgg ccgcggccga tcggcgagca ggcccgactg
     2281 caggcccagg cgctccatcg tcgcggccag gtcctttttg ccgaagcggt tgcgcggcag
     2341 gtagtaggcg gtacacatgt cccagacatc gagcatcgca aatcccggct gccgaatcgc
     2401 atcggcaatc ctgtccggca actcgccatc gaagccggtg cccctgtaga cccacggcgc
     2461 acccgccgcg atggtcacgc cgcagaggtc cagcgcgccc tcgatattgc cctgcggcgt
     2521 ggtcgcggtc agtccctcga gcggcgtggt taccgaatgc tgaccgccgg tcatgccgta
     2581 gttgaaattg ttggcgacga tgagcgtgat accgatgttg cggcgcgcga cgttgagcag
     2641 gtgcgcaccg ccgataccgc agccgccatc gcccttgaga acgataacgg tcagttccgg
     2701 acgtgccagt ttcagcccgc aggcataagt aatggagcgg ccgtgcagcc cgtgaaaggc
     2761 gtgggtgacg aagtaacggt cggcaagacc gatgcagccg atatcggtga cgatcaccac
     2821 cttgttcggt tccggctgca gactgaccag cgccttgtcg agcgccttga ccagccgatc
     2881 gtggccgcag cccgggcaga acggcaacgg gccgatttcg tcgctgagaa aagtttcgac
     2941 cgccgcgttc atttcaaacc tccctgctcg agaatttcct gcggcgaaac cagcgtggtg
     3001 tccatcttgt tgacaccgat gatctcgact cccgacggcg ccagcctctc gatttcgagg
     3061 atatactggc ccatgttcat ctccggcacg acgatccgct tgattccccg catcgccgac
     3121 accagggccg tcgccggcac gggatagagg gtttgcagca ccagcgagga aatcttcacg
     3181 ccggcggcgc gcgcgctgcg caccgcaacc gcggccgagc gcgaggtgat gccgtaactg
     3241 acgaccagta tctccgcccc cggctggaag tcctgtttca ccagcgcgat ctcgtcggcg
     3301 gccctgtcca ccttggcggc gtagtgatcg atcatttcct ggatcaccgg cgggctggcg
     3361 gcgaggtaac cgtccttgtc gtgggtggag gtggtgaagc gggcgatctg ctcgccgccg
     3421 a
//
LOCUS       DATCZV010000055         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_268888, whole genome shotgun sequence.
ACCESSION   DATCZV010000055 DATCZV010000000
VERSION     DATCZV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268888"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VIW27_01315"
     CDS             <1..888
                     /locus_tag="VIW27_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEY5738328.1"
                     /translation="MLQYEYAREALKNGLKLEQAIGVNPFKFGLVGSTDSHTGLATAE
                     EENFFGKHSGKEPHKGRMNQLVGEFGDIRYYGWEQVSSGYAAVWATENTREALWDAMQ
                     RKEVYATTGPRMTVRFFGGYDFNQRDAQLRDPGLIGYSKGVPMGGDLDGSGPGKAPNF
                     LVAAQKDPYSGNLDRIQIVKGWLDDDGRTHEKVYDVAWSDEREPGADGKLPSVGNTVD
                     VTNASWTNSIGASELISVWEDPDFDASHLAFYYARVIEIPTPRWSTYDAVRFGEEVPE
                     GVPLTTQERAYTSPIWYTP"
     gene            953..2059
                     /locus_tag="VIW27_01320"
     CDS             953..2059
                     /locus_tag="VIW27_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738329.1"
                     /translation="MRAIRQIYLLFTACVAWLKSATRYLAAIAVTCFAMLGGRAEACM
                     VCIPFPEDTATDQLLQADVVVRARENPDKPFSYVAVEVLKGELGDPRIDLFIDSTTRR
                     RLALNPEQSVLLTFSDATRDWRSAGYATPTYEALAGEILAREADWEQWGQEENRARFF
                     LPYLDDADRVVRELAYLEVGRASYETIRRADPFVPAAQVHRFLATPQYFEWHALYILL
                     LGVDAKADEKKMIRAKLASLAKFDQKRNLSAWATALIEIDGEQAIDWLEATYLGVPDR
                     DPKVVQEIAKALSVHGALQQSGLRQRIAKSYGVLIQAHPSLAGWAARDLTAWRDWRFA
                     REIRAVRESRMPIDGATAYAIDYYVGRAQSAVSN"
     gene            2184..>2714
                     /locus_tag="VIW27_01325"
     CDS             2184..>2714
                     /locus_tag="VIW27_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5738330.1"
                     /translation="MPMNIKTLWREPLLHFLLIGATLFVYYNLTADNVEAPPKRIHVE
                     RGQVEQLAANFRRTWSRPPTAQELDALVEGYVREEVFYREALAMGLDRDDPMVRRRLR
                     MKLEFMLEDLATQDVGDAVLTAYLQQNPDKFRSETELSFLQVYLNSDQRPQLEADAAE
                     MLTRLNEGADPQRLGDP"
BASE COUNT          560 a          836 c          818 g          500 t
ORIGIN      
        1 atgctgcagt acgaatatgc gcgcgaagcc ctcaagaacg gcctcaaact ggaacaggcg
       61 atcggcgtca atccgttcaa gttcggcctc gtcggctcta ccgacagcca caccggcctt
      121 gccaccgcgg aagaggaaaa cttcttcggc aagcacagcg gcaaggagcc acacaagggc
      181 cgcatgaacc agctggtcgg cgaattcggc gacatcaggt actatggctg ggaacaggtg
      241 tcttccggtt acgccgcggt ctgggccacc gaaaacaccc gcgaagcgct gtgggacgcg
      301 atgcagcgca aggaagtcta cgccaccacc ggcccgcgca tgacggtacg cttcttcggc
      361 ggctacgact ttaaccagcg cgatgcgcaa ctgcgcgacc cgggattgat cggctacagc
      421 aagggcgtgc cgatgggcgg cgatctcgac ggctccggcc caggcaaggc gccgaatttt
      481 ctcgtcgcgg cgcaaaaaga cccctatagc ggcaacctgg atcgcatcca gatcgtcaag
      541 ggctggctgg acgacgacgg caggacccac gaaaaagtct acgacgtggc ctggtcggac
      601 gagcgcgaac ccggcgccga cggcaaactg ccgtcggtcg gcaacaccgt cgacgtgacc
      661 aacgcctcgt ggaccaattc gatcggcgcc tcggaactga tcagtgtctg ggaggacccg
      721 gacttcgacg ccagtcatct cgctttctac tatgcccgcg taatcgagat accgacgccg
      781 cgctggtcga cctatgacgc tgtccgcttc ggtgaggagg tccccgaagg ggttccgctc
      841 accacccagg aacgcgccta cacctcgccg atctggtata cgccttaaat gaaataactc
      901 ggagtcggtg acaaatgatt ctcacttgtc atcgacatcc tgcagcttga aaatgcgcgc
      961 cattcgtcaa atttacctgc tttttaccgc atgcgtcgca tggttgaaaa gcgccacgcg
     1021 gtatctcgcg gccatagccg tgacttgctt cgcgatgttg gggggccgcg ccgaggcctg
     1081 catggtttgc atcccttttc ccgaggacac cgccactgac cagctgctgc aggcggacgt
     1141 ggtcgtgcgc gcgcgcgaga acccggacaa accgttctcg tacgtcgccg tcgaagtgct
     1201 caagggcgag cttggcgatc ctcggatcga tttgttcatc gacagcacga ctcgccgacg
     1261 gctggccctc aatcccgagc aatccgtgct gttgacgttc agcgacgcta cgagagactg
     1321 gcgctcagcc ggctatgcca cgccgacata cgaggcgctg gcaggcgaga tcctggcccg
     1381 ggaagcggac tgggaacaat ggggccagga ggaaaaccgg gcaaggtttt ttctccccta
     1441 cctggacgac gcggatcgcg tcgtgcgcga gctcgcctat ctcgaggtag gacgggcctc
     1501 ctacgaaacg attcggcgag ccgacccatt cgtgccagcc gcgcaggtgc atcgtttcct
     1561 ggctactccg cagtatttcg aatggcatgc actttatatt ctgttgctgg gtgtcgatgc
     1621 gaaagccgac gaaaaaaaga tgatacgtgc gaagttggcg agccttgcga agttcgatca
     1681 gaaacgcaat ctgtcggcct gggctaccgc cctgatcgaa atcgatggcg aacaggcgat
     1741 cgactggctg gaggcgacct atctcggtgt tcccgatcgc gacccgaagg tggtccagga
     1801 aatcgccaag gcactgtcgg tgcatggcgc tcttcagcaa tccgggctgc gacaacgcat
     1861 cgcgaaaagc tacggcgtgc ttattcaggc tcatccatca ctggccggct gggccgcgcg
     1921 cgacctgacc gcctggcgcg actggcgctt cgctcgcgaa atcagggcgg tacgcgagag
     1981 ccgaatgccg attgatggcg ccactgccta cgcgatcgat tactatgtcg gtcgagcgca
     2041 atcggctgtc tcaaattgat cgatggatta atgattattg gattattgga ttattggaat
     2101 ggtgacacat gattctgtct gcgattcgtc accgctccct gccctccaac gactccgggt
     2161 gaattgaaca ggaatatcac cccatgccca tgaatatcaa gacgctttgg cgcgagccgc
     2221 tgctgcattt tctgctgatc ggcgcgacgc tattcgttta ctacaacctc accgccgaca
     2281 acgtcgaggc gccgccaaaa cgcattcacg tcgagcgcgg ccaggtagaa cagctggcgg
     2341 cgaatttccg gcgcacctgg tcgcgcccgc cgacggcgca ggaactcgat gcgctggtcg
     2401 agggctacgt gcgcgaggaa gtcttttatc gcgaggcgct ggcgatgggg ctcgaccgcg
     2461 acgatccgat ggtgcgccgc cgcctgcgca tgaagctaga attcatgctc gaggacctgg
     2521 cgacgcaaga cgtcggcgac gcggtattga ccgcctacct gcagcaaaac ccggacaagt
     2581 ttcgcagcga gaccgagctc tcgttcctgc aggtctacct gaattccgac cagcgcccgc
     2641 aactggaggc tgacgcggct gaaatgctga cgcggctgaa cgagggcgcc gatccgcaac
     2701 ggctcggcga cccc
//
LOCUS       DATCZV010000056         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_639337, whole genome shotgun sequence.
ACCESSION   DATCZV010000056 DATCZV010000000
VERSION     DATCZV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639337"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="rfbB"
                     /locus_tag="VIW27_01330"
     CDS             complement(<1..971)
                     /gene="rfbB"
                     /locus_tag="VIW27_01330"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY5738331.1"
                     /translation="MKTVIVTGGAGFIGSAVIRHLIAQGGYRVINFDKLTYAGNLESL
                     VSVADHPNYHFEKGDICDHRLVKNVFIEFRPWAVMHLAAESHVDRSIDGPAEFINTNI
                     IGTSVLLEEARWYHREHEHGHDFRFHHVSTDEVFGSLGETGLFDENSPYRPNSPYSAS
                     KAASDHLVRAWHETYGLPVVVTNCSNNYGPCQFPEKLIPLVILNALEGKPLPVYGKGE
                     QVRDWLYVEDHARALQLVMEQGKTGETYNIGGHNEMRNIDVVRMLCRLLDEKVQQRPA
                     GVDSFADLIEFVTDRPGHDLRYAIDAGKIGRDLGWQPAETFESGLDKT"
     gene            complement(1241..1552)
                     /locus_tag="VIW27_01335"
     CDS             complement(1241..1552)
                     /locus_tag="VIW27_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY5738332.1"
                     /translation="MDEIHFTYILASKCRGALFVGTTDDLEGCVREHRLNQVEGLTSL
                     YAIHQLVYYEYFDKAGPALLRERDIKRLPRCRKLHLIESVNPAWRDLFNEIELAAMSP
                     A"
     gene            complement(1554..>2013)
                     /locus_tag="VIW27_01340"
     CDS             complement(1554..>2013)
                     /locus_tag="VIW27_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007447057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738333.1"
                     /translation="ARNGDPAINGGFAADYVYRNFTLGNSADEIVLRDDSLEWLRLEY
                     GPGFAIAGRSRVLDRLTMALAGYVLSPATSVYGQGDAGSPGDAGELAPALSAVPVPAA
                     AWLFGSGLLALVSRRLAADVHAGLPAVRRNPRGRARRSIATGRFYRPEAR"
BASE COUNT          384 a          636 c          600 g          393 t
ORIGIN      
        1 gtcttgtcga gcccggattc gaaagtttcg gcaggttgcc agccgagatc gcggccgatc
       61 ttgccggcat cgatcgcgta gcgcaggtcg tgccccggcc gatcggtcac gaattcgatc
      121 aaatccgcga agctgtcgac acccgccggg cgctgctgca ctttttcgtc cagcagccgg
      181 caaagcattc tcacgacatc gatattacgc atctcgttgt gaccgccgat gttgtaggtc
      241 tcgcctgttt tgccctgctc catcaccagc tgcagggcgc gtgcatgatc ctcgacatac
      301 agccagtcac gaacttgctc gcccttgccg taaaccggca gcggtttgcc ctcgagcgcg
      361 ttcaggatca ccagcgggat cagcttttcc ggaaactggc aggggccgta gttgttggaa
      421 caattggtaa ccaccaccgg taatccatag gtttcgtgcc aggcacgcac caggtgatcg
      481 gatgccgcct tgctcgccga gtacggtgaa ttggggcgat acggactgtt ctcgtcgaac
      541 agcccggtct cgccgagcga gccgaatact tcatcagtcg acacgtgatg gaatcgaaaa
      601 tcgtgcccat gctcgtgctc gcggtgatac cagcgcgctt cttcgagtaa cacgctggtg
      661 ccgatgatat tggtattgat gaattccgcg gggccgtcga tcgagcggtc gacatgcgac
      721 tcggcggcca gatgcatcac cgcccagggt cgaaattcga tgaacacgtt cttcaccagc
      781 cggtggtcgc agatgtcgcc tttctcgaag tgataattgg gatggtcggc aaccgacacc
      841 agcgattcga ggtttccggc ataggtgagc ttgtcgaaat tgatcacccg ataaccgccc
      901 tgcgcgatca ggtggcgaat gaccgccgaa ccgatgaagc cggcgccgcc ggttacgatg
      961 actgttttca aagcgcttgt tccgcgttta gtttcaactg ctaaaactag cacagccccg
     1021 tgattattcc cattgcccgg gatcacagtt tcgacaatca taccggcccg cggcctcgag
     1081 ttacggcttt ccacaagcgt tacgtcgcgc tgttcttggc ccgagaaaat atcacgccca
     1141 ctaagaagcg atcagctgcg aatttaccga gctggctgcc gtgcatgctc gtcggctcga
     1201 atcgctcgca tccgcgctcg ccggtcgccg cgcatggcag tcaggcaggc gacatcgcgg
     1261 ccagctcgat ctcgttgaaa aggtcccgcc aggccgggtt gacgctttcg atcaagtgca
     1321 gtttgcgaca acgtggcagg cgcttgatat cgcgttcgcg tagcagcgcg gggccggcct
     1381 tgtcgaagta ctcgtaataa accaactggt gaatcgcgta caggctggtc agtccctcga
     1441 cctggttgag gcgatgctcg cgaacgcagc cctcgaggtc gtcggtggtg ccgacgaaca
     1501 acgcgccgcg gcacttgctg gcgagtatat aggtaaagtg aatctcgtcc atctcagcga
     1561 gcctccgggc gataaaaccg ccccgtggcg atcgagcgcc gcgccctgcc ccgtggattg
     1621 cgccgcactg ccggcaggcc agcatgcaca tccgccgcca ggcggcgcga caccagtgcc
     1681 agcagcccgc tgccgaacaa ccaggcggcg gccggtaccg gcaccgccga caacgccggt
     1741 gccagttccc cggcatcgcc cggactgccc gcgtcgccct gcccgtagac gctggtcgcg
     1801 ggagacagca cgtagccggc gagtgccatc gttaagcggt cgagcacgcg cgaacgcccc
     1861 gcaatcgcga atcccgggcc gtattcgagc cgcagccatt ccagcgaatc gtcgcgcagc
     1921 acgatttcgt cggcgctatt gccgagagtg aaattgcggt agacgtaatc ggcagcgaag
     1981 ccgccgttga tcgccgggtc gccgttgcgc gcc
//
LOCUS       DATCZV010000057         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_428081, whole genome shotgun sequence.
ACCESSION   DATCZV010000057 DATCZV010000000
VERSION     DATCZV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428081"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /gene="mnmG"
                     /locus_tag="VIW27_01345"
     CDS             <1..1527
                     /gene="mnmG"
                     /locus_tag="VIW27_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY5738334.1"
                     /translation="QQPVVDLIENGSAIEGVVTQMGLEFYAPSVVLTVGTFLDGKIHI
                     GDSNYAGGRAGDPPATALAERLRARPFRVDRLKTGTPPRIDRKTIDYARLQEQPGDTP
                     LPLFSTMGTLDMHPEQVCCHITHTNEKTHDIIRESLHRSPLFSGEIEGVGPRYCPSIE
                     DKVVRFADKTSHQVFVEPEGLQLGEVYPNGISTSLPYDVQQRLVNSMLGFEQALITRP
                     GYAIEYDYFDPRDLRPTLETRFIEGLYFAGQINGTTGYEEAGAQGLMAGANAAARVQG
                     KKPLELKRSEAYVGVLIDDLITRGTNEPYRMFTSRAEYRLMLREDNADLRLTELGREL
                     GLVEDARWRAFEQYREQLESLNQALRDQWVRPATAAAEALEKVLQNPVTREYRIAEIL
                     KRPEIEIGDVMPFVAGADSYPQNVREQVEVNAKYAGYLARQQQEIDKASRHEQTELPD
                     TLDYTEVRGLSNEVSQKLTQHRPATIGQASRISGITPAAISLLLVHLKRHQYQVQKSA
                     "
     gene            1528..2154
                     /gene="rsmG"
                     /locus_tag="VIW27_01350"
     CDS             1528..2154
                     /gene="rsmG"
                     /locus_tag="VIW27_01350"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY5738335.1"
                     /translation="MDLAGELRAGSEELGISLQQSQYDQLLEYLRLLAKWNQVYNLTA
                     ITEPRKMLSHHLLDSLSLMPSLLQANKVLDVGSGAGLPGIPLAIAMPSTIWIMLDSNA
                     RKTRFIRQAIAHCGLRNAQVVQSRVQDYHAPDSLDFIVSRAYAPLADFCDSVTHLLTP
                     KTRLLTMKTGLKRQEAQQLDTDRFEFEEETLTVPGIGETRSLVTITPL"
     gene            2151..>2526
                     /locus_tag="VIW27_01355"
     CDS             2151..>2526
                     /locus_tag="VIW27_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5738336.1"
                     /translation="MTRTITIANQKGGVGKTTTTVNLAAALAATRRRVLLVDMDPQGN
                     ATMGSGIDKNDLKYTICDVLLGDVSARQALQHPAEAGYDVLPANADLTAAEVQLMNKI
                     GREKQLSLALDTLRDDYDYILID"
BASE COUNT          545 a          766 c          762 g          453 t
ORIGIN      
        1 cagcaaccgg tggtcgatct gatcgaaaac ggcagtgcca tcgagggcgt cgtcacgcaa
       61 atggggcttg aattttatgc gccgagcgtg gtgctcaccg tcggcacttt tctcgatggc
      121 aagatccata tcggtgactc gaactatgcc ggcggccgcg ccggggatcc gccggcgacc
      181 gcgcttgccg agcgtctgcg cgccaggccg tttcgcgtcg atcgcctgaa aacggggacc
      241 ccgccgcgta tcgatcgcaa gaccatcgac tacgcgcggc tccaggagca gccgggggac
      301 acgccgctgc cgctgttctc gacgatgggt accctcgaca tgcatcccga gcaggtttgc
      361 tgtcatatca cccataccaa cgagaaaacc cacgatatca tccgcgagag cctgcaccgc
      421 tcgccgctgt tcagcggcga aatagagggt gtcggcccgc gctactgccc atcgatcgaa
      481 gacaaggtgg tgcgattcgc cgacaagact tcgcaccagg tattcgtcga acccgaaggc
      541 ctgcagctgg gagaggtgta tcccaatggc atttccacca gcctccccta tgacgtgcag
      601 cagcggttag tcaacagcat gctcggtttc gagcaggcgt tgatcacccg gcccggctac
      661 gccatagaat acgattattt cgatccgcgc gatctgcgcc cgacgctgga aacccgcttt
      721 atcgagggcc tgtattttgc cggccagatc aatggcacca ccggctacga ggaagccggc
      781 gcgcagggtc tgatggccgg cgccaacgcc gccgcccggg tgcagggcaa gaagccgctc
      841 gagctgaagc gctccgaggc ctatgtcggc gtgctgatcg acgatctcat aacacgcggc
      901 accaatgagc cctaccgcat gttcacctcg cgcgccgagt accggttgat gctgcgcgaa
      961 gacaatgccg acctgcgtct gacggaactc ggccgtgagc tggggctggt cgaagatgcg
     1021 cgttggcggg ctttcgagca gtatcgcgag cagctcgaat cactgaacca ggcgctgagg
     1081 gatcagtggg tccgacccgc gacagccgct gccgaagccc tcgaaaaagt gttacaaaat
     1141 ccggtaaccc gggagtatcg cattgccgaa attctgaagc gccccgaaat cgaaatcggg
     1201 gacgtcatgc cgtttgtagc aggcgccgat tcgtatccgc aaaacgtgcg cgaacaggtg
     1261 gaggtcaacg ccaagtacgc cggttatctc gcgcgccagc aacaggaaat cgataaggcc
     1321 agtcggcatg agcaaaccga gttgccggac acgctcgatt acaccgaagt gcgcggactc
     1381 tccaacgagg taagccagaa actgacgcaa caccggcccg caacgattgg gcaggcgagc
     1441 cgtatttcgg gaatcacgcc ggcggcgatt tcgttgctgc tggtgcatct caagcggcat
     1501 cagtaccagg tacagaagtc ggcttgaatg gaccttgcag gcgaactccg tgccggttcc
     1561 gaggaactcg ggatctcgct gcagcagtcg caatatgacc aattgctcga atacctgcga
     1621 ctgctggcta aatggaacca ggtttataac ctcaccgcaa ttaccgagcc gcgaaaaatg
     1681 ttgagccacc acttgctcga cagtctgtcg ctgatgccgt cgctgctgca ggccaacaag
     1741 gtgctagacg ttggcagcgg cgccggcctg ccgggaatac cgctggccat agctatgccc
     1801 tcgaccatct ggatcatgct cgatagcaat gcgcgtaaga cgcgttttat tcgccaggcg
     1861 atcgcgcatt gcggcctgcg caatgcgcaa gttgtgcagt cgcgggttca ggactatcat
     1921 gccccggatt cgctggattt catcgtcagc cgtgcttacg cgccgttggc cgatttctgc
     1981 gacagtgtta cgcatttgct gacgccgaaa acccggttgc tgacgatgaa gaccgggctc
     2041 aagcgacagg aggcgcagca gctggatacc gacagattcg aattcgagga agagacgttg
     2101 acagtgcccg gcatcggcga aacccgcagc ctggtgacga ttacaccgct atgacccgga
     2161 ctattaccat tgccaatcag aagggcgggg tcggcaagac cacgaccacg gtcaacctgg
     2221 ccgcggcgct ggccgcgacc cggcgccgcg tgctgctggt cgatatggat ccgcagggca
     2281 acgcgacgat gggcagcggc atcgacaaga acgatctgaa atacactatc tgcgatgtat
     2341 tactcggtga cgtcagcgcc aggcaggcgc tgcagcaccc cgccgaggcg ggctacgacg
     2401 tgctgcccgc taatgccgat ctgaccgcgg ccgaggtgca gttgatgaac aagattggcc
     2461 gcgaaaaaca gctttcactg gcgctcgaca cgctgcgcga cgattacgat tacatactga
     2521 tagact
//
LOCUS       DATCZV010000058         3331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_4512, whole genome shotgun sequence.
ACCESSION   DATCZV010000058 DATCZV010000000
VERSION     DATCZV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4512"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIW27_01360"
     CDS             complement(<1..410)
                     /locus_tag="VIW27_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009114418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5738337.1"
                     /translation="MQTRQLGTDGPRVGALGLGCMGMTPIYDTPDPDEAIATIHAAVD
                     AGATMIDTADMYGGGKNEELVGRGIKGIRERVFLASKFGNMRLPDGTRKVNGKPEYVF
                     EACEKSLARLGVDHIDLYYLHRMDPTVSSIGTGGS"
     gene            complement(495..1769)
                     /locus_tag="VIW27_01365"
     CDS             complement(495..1769)
                     /locus_tag="VIW27_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEY5738338.1"
                     /translation="MKVQLAAGVERFAHLDIEGGGQVVVDGNYAFIGHMKPPHGTSIV
                     DVSDPRRPRVCARLEIGRWSHSHKVRVCGELMITNVEQDRRHYLRRGSARLTAAREDL
                     ARRLGRAPEAAEIAAELGIDKTELEELEAALERGYDEGGFKVWNIADPAKPRLLCHRR
                     THGFGVHRFDMDERYAYISTEMPGYIGNILVIYDLADPQNPAEVSRWWMPGQHLDGGE
                     TPTWQGYSHRLHHALRAGDELWAAVWHAGFRVIDISDITRPRTRAAHTYHPWVVEPTH
                     TVLPASRPMGGRRVAVVADEEHDHRHGQPHAGLWVFDVSDLDAITPLSTFHVSELDSP
                     WARTGGRFGMHQFQEHIDDSLVYCAWFAGGLRIVDIGDPERPVEAGFYLPEPVAGTDY
                     PQSNDVDVDARGLIYLLDRNRGLDILEYRLHG"
     gene            complement(1766..3289)
                     /locus_tag="VIW27_01370"
     CDS             complement(1766..3289)
                     /locus_tag="VIW27_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY5738339.1"
                     /translation="MAPDLSVFFSLFVDTLDYWWIIIPGTLLGILVGAIPGFSAANTI
                     IVLLPLTLSMSPVNGMIFMVALYTASRLGAGIPAILVNIPGTAGAAATPLDGYPMAVA
                     GRGQQALAISFTASVAAGLITTIAALILIPWLSQVAYHLHSVEMIVVMLFGISLIATI
                     SAEDQIKGLIAGFFGLMIGYIGADVVYEAPRGTFGFLELYDKVPLIPALVGLFAISEA
                     FVVIERESILSEQGAIRQQGSGWGPTFEGIRDTLRHWKEVAWTAMLGLVIGVIPGAGA
                     AIASFVAYQQSRLMSKTPEKYGTGHVPGLIAPEAANNGVTSGTLIPLLIIGVPGGATA
                     AIMAVVLEYHGVNFGPGLFSSEPMIAYGSFTAMLITYLLLGLMILPLTRYFSRVMVVP
                     TVYMAPILIAFTLVGSFVPRAYIFDMGIAIVFGVIGYIARRTGYHVAAILIGVILGPL
                     LENYFLRALRIAQGDLTVLFSSNIGNTLWVLLILSLLAPSLKRLVQRRRAAREGSTS"
BASE COUNT          632 a         1063 c         1091 g          545 t
ORIGIN      
        1 gacccgccgg tgccgatcga ggataccgtc gggtccatgc ggtgcaggta atagagatcg
       61 atgtgatcga caccgaggcg agcgagcgat ttttcgcagg cctcgaaaac atactcgggc
      121 ttgccgttga ccttgcgcgt gccgtcgggc aggcgcatgt taccgaactt gctcgccagg
      181 aatacgcgct cgcgaatgcc tttgatgccg cgccctacca gttcctcgtt cttgccgccg
      241 ccgtacatgt cggcggtatc gatcatcgtc gcgccggcat cgactgcggc gtgaatcgtc
      301 gcaatagcct cgtcggggtc cggcgtgtcg tagatcggcg tcatgcccat gcaccccagg
      361 ccgagggcgc cgactcgcgg gccgtcggtt ccaagttgtc tcgtctgcat tttctttccc
      421 ccgtttattg gcggtcaata gcgaaggcgc ctgccgcggg caaagcgcga ttatacggta
      481 tgtgcggctt gccattagcc atgcagccgg tactcgagta tatctaggcc gcggttgcgg
      541 tcgagcaggt agatcaggcc gcgcgcatcg acgtcgacgt cgttgctctg cgggtagtcg
      601 gtgccggcca ccggctcggg tagataaaaa ccggcttcga cgggccgctc gggatcgccg
      661 atgtcgacga tgcgcaggcc gccggcgaac cacgcgcagt agaccagcga atcgtcgata
      721 tgctcctgga actggtgcat gccgaagcgc ccgccggtcc gcgcccacgg cgagtcgagc
      781 tcgctgacgt ggaacgtcga cagcggtgtg atcgcatcga gatcgctgac gtcgaacacc
      841 catagcccgg cgtgcggctg cccgtggcgg tggtcgtgtt cctcgtcggc gaccaccgcg
      901 actcgacggc cgcccattgg ccggctcgcc ggcagcacgg tatgcgtcgg ctccaccacc
      961 cacggatggt aggtatgcgc agcgcgggtt cgcgggcgag tgatatcgct gatatcgata
     1021 acccgaaagc cggcatgcca gaccgcagcc cacagttcgt caccggcgcg caacgcatgg
     1081 tgcaggcggt gggaatagcc ttgccaggtc ggcgtctcgc cgccgtcgag atgctggccg
     1141 ggcatccacc agcgggaaac ctcggcggga ttttgcggat cggcgaggtc gtagatgacc
     1201 agtatattgc cgatataccc cggcatctcg gtggagatat aggcgtagcg ctcgtccata
     1261 tcgaaacggt gcacgccgaa gccgtgcgtg cgccggtggc aaagcaggcg aggcttcgcc
     1321 gggtcggcga tgttccagac cttgaagccg ccctcgtcgt aaccgcgctc gagtgcggct
     1381 tcaagctcct ccagctcggt tttatcgata ccgagttcgg cagcgatttc ggcggcctcg
     1441 ggcgcccgac ccaggcgccg cgcgaggtct tcgcgggcgg cagtcaggcg agcgctgccg
     1501 cggcgcaggt aatgacgccg atcctgttcg acgttggtga tcatcaactc accgcaaacc
     1561 cgcaccttgt ggctgtgcga ccagcggccg atctccaggc gcgcgcaaac tcgcgggcgg
     1621 cgcggatcgg aaacgtcgac gatgctggtg ccgtgcggcg gcttcatgtg gccgatgaag
     1681 gcgtaattgc cgtcgaccac gacctgcccg ccgccctcga tgtcgaggtg cgcgaagcgc
     1741 tcgaccccgg cggcgagttg caccttcacg aggtgctacc ctcgcgcgcg gcgcggcggc
     1801 gctgcaccag gcgcttgagg ctcggcgcca gcaacgacag gatcagcagc acccacaggg
     1861 tgttgccgat gttcgacgaa aacaacaccg tcaggtcacc ctgggcgatg cgcagggcgc
     1921 gcagaaaata gttttccagc agcgggccga ggatcacgcc gatcaggatc gcggccacgt
     1981 ggtagccggt gcggcgcgcg atataaccga tgacgccgaa cacgatcgcg atccccatgt
     2041 cgaaaatata ggcgcgcggt acgaacgagc cgaccagcgt gaaagcgatc aggatcggcg
     2101 ccatgtacac cgtcggcacc accatgacgc gcgagaaata acgcgtcagc ggcaggatca
     2161 tcaatcccag cagcaggtag gtgatcagca tcgcggtaaa cgagccatag gcgatcatcg
     2221 gttcggaact gaacagaccc gggccgaaat tgacgccgtg gtattcgagc accacggcca
     2281 tgatcgccgc cgtggcgccg ccaggcacgc cgatgatcaa cagcggaatc agcgtgcccg
     2341 aagtgacacc gttgttggcc gcctcggggg cgatcaggcc gggcacgtgg ccggtgccgt
     2401 atttctccgg cgtcttcgac atcaggcgcg actgctggta ggcgacgaac gacgcgatcg
     2461 cagcgccggc tccgggaatg acgccgatca ccagccccag catcgcagtc caggcgacct
     2521 ccttccagtg cctcaaggtg tcgcgaatac cctcgaaggt cggtccccag ccgctgccct
     2581 gctggcggat cgcgccctgc tcggagagga ttgattcgcg ctcgatgacg acgaaggcct
     2641 cggaaatcgc gaacagcccg accagcgcgg ggatcagcgg caccttgtcg taaagctcga
     2701 gaaaaccaaa ggtgccgcga ggcgcctcgt agaccacgtc ggcgccgatg tagccgatca
     2761 tcaggccgaa aaaccccgca atcaggccct tgatctggtc ttccgcggag atggtcgcga
     2821 tcagcgagat accgaacagc atgaccacga tcatctccac gctgtgcagg tgatacgcga
     2881 cctgcgacag ccacggaatc aggatcagcg ccgcgatcgt ggtaatcagg ccggcggcga
     2941 ccgaggcggt gaacgagatg gccagcgcct gctggccgcg gccggcgacc gccatcgggt
     3001 agccgtcgag cggcgtcgcc gcggcgccgg cggtacccgg gatattgacc aggatcgccg
     3061 gtataccggc gccgagccgc gacgcggtgt acagcgcgac catgaagatc atgccgttga
     3121 ccggactcat cgacagcgtc aacggcaaca gcacgatgat cgtgttggcg gcgctgaaac
     3181 cgggaatcgc accgacgagt atgcccagca gcgtgcccgg aatgatgatc caccagtagt
     3241 cgagcgtatc gacgaacagc gaaaaaaata cgctcaggtc gggtgccatg acttacagca
     3301 ggcccttgag ccagtcctcg atcggaccgc t
//
LOCUS       DATCZV010000059         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_586731, whole genome shotgun sequence.
ACCESSION   DATCZV010000059 DATCZV010000000
VERSION     DATCZV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586731"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..306)
                     /locus_tag="VIW27_01375"
     CDS             complement(61..306)
                     /locus_tag="VIW27_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY5738340.1"
                     /translation="MTPEKIQQLIAQQMPDATVEVSGGEGKFVATVTSDAFDGLTPLK
                     RHKLVYACVNDQIASGELHALTIVAKTPAELGRAAGG"
     gene            468..1181
                     /locus_tag="VIW27_01380"
     CDS             468..1181
                     /locus_tag="VIW27_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEY5738341.1"
                     /translation="MRLHDLFETIGLTGHRPSPSEITISTLGGFFGILAILLVSRWWL
                     DAATAIIIVPSMGASAVLLFAAPNAPFSQPWNVAAGHILSALVGVACWQWIPELVLAA
                     SAGVGLAIGAMYAARCIHPPGGATALAAVIGSEQLHALGFGYVLQPILLNVAIILLIA
                     VVFNGLFERRRYPARQPFQIPAERAKQQDYPPINHEDFVYALSQLDTFIDVSEDDLLK
                     IYSLATRRHQQQAPHHADT"
     gene            complement(1198..>2194)
                     /locus_tag="VIW27_01385"
     CDS             complement(1198..>2194)
                     /locus_tag="VIW27_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY5738342.1"
                     /translation="FACIDPLAIDDFTPLAGLLQKQSLLKVFHSCSQDLEILYQRFGR
                     VPVPVFDTQLAAAVLGYHHQVSYADLVKQVTGVALEKKHTRANWMRRPLSRDELDYAM
                     DDVRYLLPVYRQLDAELEGRQRRSWIENELRTISDPANYEVDTAQLWKRLRGVQKLKG
                     VKLQIASDLCRWREQQARQQNRPRRWIAKDDALIEIARRRPASLEALRQIPELGDKTV
                     KRHGDKLLAIVAEAAGVDVGEWPRHEQIQALDSAQTALGDCLMALCRVIADNNGIALA
                     TLATRKDIDNLVLNRKSSRLTQGWRFAMAGEQLLEFIHGQAVIAVNDRQIRIDPK"
BASE COUNT          373 a          691 c          676 g          454 t
ORIGIN      
        1 gtcaatgaaa tgatcacgcc gcggcgatcg ctagaccgcc gccgttgact gctgctgcgc
       61 tcagccgccc gcggcccggc cgagctcggc cggcgttttg gcgacgatgg tcagtgcatg
      121 cagctccccg gaggcgatct ggtcgttgac gcaggcgtac accagcttgt ggcgcttgag
      181 cggtgtcagg ccgtcgaacg catcgctggt gacagtggcg acgaacttgc cttcgccgcc
      241 gctgacttcc accgtcgcat cgggcatctg ctgggcgatt aactgctgga tcttttcggg
      301 ggtcatgaaa ctggttccgg gatagacaat aaccgaaact atattacatc aggccccggg
      361 aaaaaccatg ccggcagcgg ttgtatcgcc gcggcatggc gtgcgcccgc agcgccggcg
      421 atcggtgtat tatttccgag aatattcaaa gcggcccgga gacctctatg cgcctgcacg
      481 atcttttcga gacgattggt ttgaccggtc acaggcccag ccccagcgaa atcacgatat
      541 caacgctcgg cggttttttc ggcatcctgg cgatactgct ggtcagtcgc tggtggctcg
      601 atgcggcgac cgcaatcatc atcgtgccat cgatgggcgc cagcgcggta ctgctgttcg
      661 cggcccccaa tgctcccttt tcacaaccct ggaacgtcgc cgccgggcac atcctgtcgg
      721 ccctggtcgg cgttgcctgc tggcaatgga ttcccgagct tgtgctcgcc gcgtcggccg
      781 gcgtagggct ggcgatcggc gcgatgtatg cggcgcgctg cattcatccc ccgggcggcg
      841 ctaccgcgct ggctgcggtg atcggctccg agcaactgca tgccctgggt ttcggctacg
      901 tgttgcaacc catcttactg aatgtggcga ttattctgct gatcgcggta gtgttcaacg
      961 gcctgttcga gcggcggcgg tacccggctc gacagccttt tcaaataccg gccgagcgcg
     1021 cgaagcagca agactaccca ccgatcaatc acgaggattt cgtctatgcg ctcagtcagc
     1081 tggatacttt tatcgatgtt tcggaagacg atttgctgaa gatctactcc ctggcgaccc
     1141 ggcggcacca gcaacaggca ccgcaccacg ccgatacctg acagcacccg ggcggcgcta
     1201 cttcggatcg atgcggattt gtcgatcgtt gactgcgatc accgcctggc cgtggatgaa
     1261 ttcgagcaac tgctcgccgg ccatcgcgaa gcgccagccc tgggtcaggc gactgctctt
     1321 gcggttgagc accaggttgt cgatatcctt gcgggtggcc agtgtcgcca gcgcgatacc
     1381 gttgttatcg gcgattactc gacatagcgc catcaggcaa tcgccgagcg ccgtctgcgc
     1441 ggaatctagc gcctgaatct gctcatgtcg tggccattcg ccgacatcga caccggccgc
     1501 ctcggcgacg atcgccaaca gtttgtcgcc gtggcgcttg accgtcttgt cgccgagttc
     1561 gggaatttgc ctgagcgcct ccaggctcgc cggccggcgg cgcgcgatct cgatgagcgc
     1621 atcgtccttg gcgatccagc gccgcggccg gttttgctgg cgtgcctgtt gttcgcgcca
     1681 gcggcaaagg tcgctggcga tctgcagctt gacgcccttg agtttctgca cgccgcgcag
     1741 gcgcttccac agttgcgccg tatcaacctc gtaattggcc gggtcgctga tggtccgcaa
     1801 ttcgttctcg atccagctgc gccgctggcg gccttcgagt tcggcatcca gctgccggta
     1861 gaccggcaac aggtagcgca cgtcgtccat cgcgtagtcg agttcgtcgc gcgatagcgg
     1921 ccgccgcatc cagttggcgc gagtatgctt tttctccagc gccacgccgg tcacctgctt
     1981 taccaggtcg gcataggaca cctggtgatg ataaccgagc accgcggcgg caagctgggt
     2041 atcgaacacc ggcaccggca cacggccaaa gcgctggtag agaatttcca ggtcctgcga
     2101 acaggagtgg aaaaccttca gcaggctctg cttttgcagc aggccggcaa gcggtgtgaa
     2161 atcatcgatc gccagcggat cgatgcaggc gaat
//
LOCUS       DATCZV010000060         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_375033, whole genome shotgun sequence.
ACCESSION   DATCZV010000060 DATCZV010000000
VERSION     DATCZV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375033"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VIW27_01390"
     CDS             complement(<1..1108)
                     /locus_tag="VIW27_01390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5738343.1"
                     /translation="MTAPFVSPDLMPALPEMVMLAMTCLVLVVDLFLPQQRRGFTLLL
                     SLATLVLAVFAVIAVAPTGSVTSFSGSFVLDQLAVVLKIAVCGVTFLVFVYSRDYLID
                     NAIYKGEYYVLGLFATLGMMVMISAQSFLLIYLGLELLSLSLYSMIAFNRDSLRASES
                     AMKYFVLGAMASGLLLYGISIFYGVTGTINLRQLAVAVDSQMALHPVALGFALTFIVV
                     GLSFKLGAVPFHMWLPDIYQGSPTSVTLFIGTAPKIAGFAMAIRLLVDGLGDLAFDWS
                     QMLTALAIASMALGNVIAIAQTNFKRMLAYSTISHVGFILLGLLSATANGYASAMFYT
                     ITYAITAAVAFGVLIVLNRKGFEAENISDLSGLND"
     gene            complement(1105..>2174)
                     /locus_tag="VIW27_01395"
     CDS             complement(1105..>2174)
                     /locus_tag="VIW27_01395"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5738344.1"
                     /translation="FLVIGIWGGANRIYATLKFFLYTFIGSVLMLVALIYMYTQSNSF
                     AIFDFHHLRLGLTEQVLIFLAFLVAFAVKVPMWPVHTWLPDAHVEAPTGGSVILAAIM
                     LKVGGYGFLRFSLPITPDASAELDWLIITMSLTAVVYIGFVALVQRDMKKLIAYSSIA
                     HMGFVTLGFFIVFRIAASDFGNQGAGLALEGGMVQMISHGFISGALFLCVGVVYDRMH
                     SRQIADYGGVANTMPVFAAFMVFFAMANAGLPGTSGFVGEFMVILASFKASFWIAFLA
                     ALTLILGAAYTLWMIKRVVFGEVANDNVAALEDINRREFLFLAILALAVLGLGLWPDP
                     LVEVMHPTMENLIDHIANSKI"
BASE COUNT          516 a          671 c          669 g          318 t
ORIGIN      
        1 catcgttcag gcccgacagg tcactgatgt tctcggcctc gaatcccttg cgattgagga
       61 cgatcaacac gccgaaagcg accgcggcgg tgatcgcgta ggtaatggta taaaacattg
      121 ccgacgcgta accgttggcg gtcgccgaca acaggccgag caggatgaag ccgacatgcg
      181 atatcgtcga gtaggccagc atgcgcttga aattcgtctg cgcgatggcg atgacattgc
      241 cgagcgccat cgacgcgatc gccagcgcgg tcagcatctg cgaccagtcg aaggccagat
      301 cgccgagccc gtcgaccagc agcctgatcg ccatcgcgaa gccggcgatc ttcggcgccg
      361 tgccgatgaa cagcgtcacc gaggtcggcg aaccctggta gatatccggc agccacatgt
      421 gaaacggtac cgcgccgagc ttgaacgaca ggccgacgac gatgaaggtc agcgcgaatc
      481 ccagcgctac cgggtgcaat gccatctggc tgtcgaccgc gacggccagc tggcgaagat
      541 tgatggtgcc ggtgacgcca tagaaaatcg agatgccgta gagcagcagc ccggaggcca
      601 tcgcgccaag cacgaagtat ttcatcgccg attccgaggc gcgcagcgaa tcgcggttga
      661 aggcgatcat cgagtacagc gacaacgaca acagctccag gccgagatag atcagcagga
      721 aggactgcgc cgagatcatc accatcatgc cgagcgtcgc gaacagcccg agcacgtagt
      781 actcgccctt gtagatcgcg ttgtcgatga ggtaatcgcg cgaatagacg aacaccagga
      841 aggtcacccc gcagaccgcg atcttcaaca ccacggccag ctgatcgagc acgaacgagc
      901 cgctgaaact ggtgaccgag ccggtcggcg cgaccgcgat caccgcgaac acggccagca
      961 ccagcgtggc cagcgacaac agcaaggtga acccgcgccg ctgctgcggc aggaacaggt
     1021 cgaccaccag caccaggcag gtcatcgcca gcattaccat ctccggcagc gcgggcatca
     1081 ggtcgggcga tacgaaaggc gcagtcatat cttcgagttc gctatgtgat caatcaggtt
     1141 ttccatcgtc gggtgcatca cctcgaccag cgggtccggc cacaggccga ggccgagtac
     1201 cgccagcgcc aggatcgcga ggaacaggaa ctcgcggcgg ttgatgtcct cgagcgcggc
     1261 gacattgtcg ttggcgacct cgccgaagac tacccgcttg atcatccaca gcgtatacgc
     1321 ggcgccgagg atcagcgtca gcgcggcgag gaacgcgatc cagaaactgg ccttgaagct
     1381 cgccaggatg accatgaatt caccgacgaa gcccgaggtt cccggcaggc cggcgttggc
     1441 catggcgaag aacaccatga aggccgcgaa caccggcatg gtattggcga cgccgccgta
     1501 atcggcgatc tggcggctat gcatgcggtc gtagacgacg ccgacgcaga ggaacagcgc
     1561 gccggagatg aaaccgtggg agatcatctg caccatgccg ccctcgagcg ccaggcccgc
     1621 gccctggttg ccgaagtccg acgccgcgat acggaacacg atgaagaaac cgagggttac
     1681 gaaacccatg tgagcgatcg acgaataggc gatcagcttt ttcatgtcgc gctgcaccag
     1741 cgccacgaaa ccaatataga ccaccgcggt cagcgacatg gtgatgatca gccagtcgag
     1801 ctcggcgctg gcgtccggcg tgatcggcag actgaaacgc aggaatccgt agccgccgac
     1861 cttcaacatg atcgccgcca ggatcaccga gccgccggta ggcgcctcga cgtgcgcgtc
     1921 aggcagccag gtatgcaccg gccacatcgg caccttgacc gcgaacgcaa ccaggaaggc
     1981 gaggaagatc agtacctgct cggtcaggcc gagcctcagg tgatgaaaat cgaagatcgc
     2041 gaaactgttc gactgcgtgt acatgtaaat cagcgcgacc agcatcagca ccgagccgat
     2101 gaaggtgtag agaaagaact tcagcgtcgc gtagatgcgg ttggcaccac cccagatacc
     2161 gatcaccagg aaca
//
LOCUS       DATCZV010000061         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_534261, whole genome shotgun sequence.
ACCESSION   DATCZV010000061 DATCZV010000000
VERSION     DATCZV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534261"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VIW27_01400"
     CDS             complement(<1..255)
                     /locus_tag="VIW27_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738345.1"
                     /translation="MSDQLFEIAFSGRISDGANPAEVRANIGKMFNADEGKLAQLFSG
                     KRIVIKKNLDQATAVKYKSAFNRAGAECEVRPMGGTPAAQP"
     gene            314..1690
                     /gene="ppnN"
                     /locus_tag="VIW27_01405"
     CDS             314..1690
                     /gene="ppnN"
                     /locus_tag="VIW27_01405"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HEY5738346.1"
                     /translation="MTRKPKITRLTPRGRMDILSRLEVNQLKQGSADDTLYETFRRCA
                     LAVLNVDSQTDDAREVLEQYRDFDIRVIQQERGLKLEIENAPDSAFVDGQVIRGIQEQ
                     LFAVLRDVSYVKNKIESNRIFDIATSEGITNVVFHILRNARVLVPEIEPNLVVCWGGH
                     SIGRAEYDYCKEIGYQLGLRGLDICTGCGPGAMKAPMKGAAIGHSKQRIREGRYVGLT
                     EPGIIAAESPNPIVNELVILPDIEKRLEAFVRAGHAFVVFPGGVGTTEEILFLLGILL
                     NPENRKIPFPLILTGPESARQYFEQVDDFIKLTLGRAAQQHYNIVIGEPVRVAREIAA
                     GLESVRNFRREFSDAYYFNWILKIDHPFQQPFEPSHENVAALDIDRGREPHLLAADLR
                     RAFSAIVAGNVKDDGIRRIEQHGPFEIRADNDIMLALDRLLHAFVEQGRMKVPGKTYV
                     PCYSLHKR"
     gene            1731..2180
                     /locus_tag="VIW27_01410"
     CDS             1731..2180
                     /locus_tag="VIW27_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5738347.1"
                     /translation="MSRVRAIAIRNRPRAAMQSIDSARIDIASGILGDFRGSQRDRQV
                     TILSESAWQRACDEVGAELPWTVRRANLLVDGIEFDDSYIGKRVRIGEAELEITEETD
                     PCTRMDEQHPGLSAALEPEWRGGVCCRVLKAGDIRIGDAVEIANGDS"
     gene            complement(2241..>2937)
                     /locus_tag="VIW27_01415"
     CDS             complement(2241..>2937)
                     /locus_tag="VIW27_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5738348.1"
                     /translation="IGRPGLWAALVLGLGSICYIYAILWTTVANAVFIIGSAPLATAF
                     VGWLVLGEKTPRFGVAAMLVSLCGIGLMFADGLLEGRWLGNVAAFGVVASFVVFLMII
                     RSRRGVDMLPATCLSGVVMGSVAWLFVESLVLTPRDLAITITMGCLQFGVGFWCFTVA
                     SRYIMASEVALFSLSESILAPIWVWIGVGEQPSLLTLAGSAVVLVSVSAYCVSGIRAE
                     RRLLRDARRFEQA"
BASE COUNT          612 a          943 c          887 g          495 t
ORIGIN      
        1 cggttgtgct gccggcgtcc cgcccatggg cctgacttcg cactcggcgc ccgcgcggtt
       61 gaacgcggac ttgtacttga cggcggtcgc ctggtcgaga tttttcttga tgacgatacg
      121 cttgccggaa aacagctgcg ccagcttgcc ctcgtcagca ttgaacatct tgccgatatt
      181 ggccctgact tccgccgggt tggcgccgtc gctaatcctg cccgaaaaag cgatttcgaa
      241 taattgatcg ctcatgtcat atttctcgtg actactgcat tataatatag aacatatttg
      301 gccagcctgt agcatgaccc ggaaacccaa gatcactcgc ctgaccccgc gcggccgtat
      361 ggatatcctg tcgcggctcg aggtcaacca gctcaagcag ggcagcgccg acgacaccct
      421 ctacgaaacc ttccggcgct gcgcgctggc ggtgctgaat gtcgacagcc agaccgacga
      481 tgcacgcgag gtactcgagc aataccgcga tttcgatatt cgtgtcattc aacaggagcg
      541 tggtctgaag ctggaaatcg agaacgcgcc cgacagtgcg ttcgtcgacg gccaggtgat
      601 ccgcggcata caggaacaac tgttcgcggt gctgcgcgac gtttcctacg tcaagaacaa
      661 gatcgaatca aaccggattt tcgatatcgc gaccagcgag ggcatcacca acgtcgtctt
      721 ccatatcctg cgcaacgccc gtgtgctggt cccggaaatc gagcccaacc tggtggtctg
      781 ctggggcggg cactcgatcg ggcgtgccga gtacgactac tgcaaggaga tcggttacca
      841 gctcggtctg cgcggactgg atatctgcac cggctgcgga ccgggggcga tgaaagcgcc
      901 gatgaagggc gccgcgatcg gccacagtaa acagcgcatt cgcgaggggc gctatgtcgg
      961 tctcaccgaa cccggcatca ttgccgccga gtcgccgaat ccgatcgtca acgagctggt
     1021 cattctgccg gacatcgaga aacgcctgga agccttcgtg cgggccgggc atgccttcgt
     1081 ggtgtttcct ggcggcgtcg gcaccaccga ggaaatcctc ttcctgctcg gcatcctgct
     1141 caatcccgag aatcgaaaaa ttccctttcc cctgatcctc accgggccgg aaagcgcgcg
     1201 gcaatacttc gagcaggtcg acgacttcat caagctgaca ctcgggcgcg ccgcgcagca
     1261 gcactacaat atcgtcatcg gcgagccggt tagagtggcc cgcgagatcg ccgccgggct
     1321 cgagtcggtg cgcaatttcc gccgagaatt ttccgacgct tattatttca actggatact
     1381 gaagatagac cacccgttcc agcaaccatt cgagcccagc catgaaaacg tcgccgcact
     1441 cgatatcgac cgcggccgcg agcctcacct gctggcggcc gacctgcggc gcgcgttctc
     1501 ggcgatcgtc gccggcaatg tcaaggacga cggcattcgc cgcatcgaac aacacggccc
     1561 gttcgagatt cgcgccgaca acgacatcat gctggcgctc gaccgtttgc tgcacgcgtt
     1621 cgtcgaacaa ggccgcatga aggtgccggg taaaacctac gtgccttgtt attcgctgca
     1681 taaacgctga caatcccggt ttttcgataa gcggatttgg caggtaatca atgagcaggg
     1741 tcagggcaat cgccatcagg aatcgtccgc gcgcggcgat gcagtcgatc gacagcgcgc
     1801 gcatcgatat cgccagcggc attctcggtg atttccgcgg cagccagcgg gaccggcagg
     1861 tcaccatcct cagcgaatcc gcgtggcaac gggcctgcga cgaggtcggc gccgagctgc
     1921 catggaccgt gcgtcgcgcc aacctgctgg tcgacggcat cgagttcgac gacagttata
     1981 tcggcaagcg cgtgcgcatc ggcgaggccg agctggaaat aaccgaggaa accgatccct
     2041 gcacgaggat ggacgagcag cacccgggac tgagcgcggc gctcgaaccc gagtggcgcg
     2101 gcggggtttg ctgccgggta ctgaaggccg gcgatattcg catcggcgac gcggttgaaa
     2161 tcgctaacgg cgattcctga gcgcaacgca gcgacaccgg cgcggtcgat cgccgcggcc
     2221 ggcttcgagc gcgctcggga tcaggcctgc tcgaatcgcc tggcgtcccg caacaggcgg
     2281 cgctcggcgc gaatcccgct gacacagtag gcgctgacgc tgaccagcac cacggcgctg
     2341 cccgccagcg tcagcaggct gggctgctcg ccgaccccga tccagaccca gatcggcgcc
     2401 aggatcgatt cgctgagcga gaacagcgcc acctccgagg ccatgatgta tcgcgaagcc
     2461 acggtaaaac accagaagcc gacaccgaac tgcaggcaac ccatggtgat cgtgatcgcc
     2521 aggtcgcgcg gcgtcagtac cagcgactcg acgaacagcc atgccacgct gcccatgacg
     2581 acgccggaaa ggcaggtcgc cggcagcatg tcgacgccgc gccggctgcg aatgatcatc
     2641 aggaagacaa cgaacgaggc aaccacgccg aaggcggcaa cattgcccag ccagcgcccc
     2701 tcgagcaaac cgtcggcgaa catcaggccg ataccgcaaa gcgacaccag catcgcagcg
     2761 acgccgaaac gcggggtttt ttcgccgagc accagccaac cgacgaacgc ggtcgccagc
     2821 ggcgccgaac cgatgatgaa cacggcgttg gcgaccgtgg tccagaggat tgcgtagata
     2881 tagcaaatcg atcccaggcc gagcaccagc gcggcccaga gtccgggcct gccgatc
//
LOCUS       DATCZV010000062         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_110522, whole genome shotgun sequence.
ACCESSION   DATCZV010000062 DATCZV010000000
VERSION     DATCZV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110522"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VIW27_01420"
     CDS             complement(<1..542)
                     /locus_tag="VIW27_01420"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEY5738349.1"
                     /translation="MPKPSHLFSLLVLNALPLIGVIAWDWQSFDLIFLYWLENLIIGA
                     FTLLRMLIRPYRHAADFVVALFFAPFFTFHYGMFCLAHGSFVFALFGPEDFAMQSFAG
                     ALLQIRPTLEGNHLGWAALSLLLLQLVDWGRDVRRHGLGFDGIKTLMLAPYRRIIVLH
                     VAILGSGFVLAALDEPVLGLA"
     gene            1477..>2247
                     /gene="mdh"
                     /locus_tag="VIW27_01425"
     CDS             1477..>2247
                     /gene="mdh"
                     /locus_tag="VIW27_01425"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5738350.1"
                     /translation="MKVCVLGAAGGIGQPLSLLLKLQLPAGSELSLYDVAPFTPGVAV
                     DLSHIPTDVCVTGYVGDDVEAAMKDADIVIIPAGVPRKPGMTRDDLFNMNAGIVKKLI
                     GVGAKACPGALFGIVTNPVNSTVPIAAEVLKAAGVYDKRKLFGVTTLDVIRSNTFVAQ
                     TAGKSTGEVNVNVIGGHSGVTILPLLSSVPGVSLSQADIEALTPRIQEAGTEVVEAKA
                     GAGSATLSMAQAAARFTMSLVAALGGESVTECCYVQVGD"
BASE COUNT          458 a          637 c          687 g          465 t
ORIGIN      
        1 gcgaggccga ggaccggctc gtcgagcgcg gcgagcacga aacccgaacc caggatcgcg
       61 acgtgcaata cgatgatgcg ccgatagggc gccagcatca gggtcttgat gccgtcgaaa
      121 ccgagaccgt gccggcgaac gtcgcgcccc cagtcgacca gttgcagcag cagcaggctc
      181 aacgccgccc agcccaggtg gttgccttca agcgtcggcc ggatttgcag tagcgcgccg
      241 gcaaagcttt gcatggcgaa atcctcggga ccgaacaggg cgaatacgaa gctaccgtgg
      301 gcgaggcaga acatgccgta gtgaaaagtg aaaaacggcg cgaaaaacag cgcgacgacg
      361 aagtcggcgg cgtgccggta gggccggatc agcatacgca acagtgtgaa tgcgccgatg
      421 atcaggttct ccagccagta aagaaaaatc aggtcgaaac tctgccagtc ccaggcgata
      481 acgccgatca gcggcagcgc gttcaacacc agcagggaaa agagatggga aggtttcggc
      541 atatcagccc tggtttgcgt atcaagcgca ggcgcaaagc gattactatc cggggtcagg
      601 ctattgtccg ctgcgccact gttttacact ttaaccacaa gtttagtcga tggtatagaa
      661 agcataaccc gcctgtttgc tccagagcca gtcgatctgc ctggataacc agggctctgg
      721 tgctcgttgg gttttctgcg tgatcgggtt tggcagtatc agtctggcga ttgttgggtt
      781 tttcagatgc cttttcaggt agatctcctg gtacgaagca gcttaccagg cgagtccgtt
      841 tgggctgctt gaaaatgtga tttctggctt gcgttgatct gaagggctga accgcgggag
      901 gccctgtgct ctcaggaatc aggcccatcg gtgtaaatca ggttcctttc cagtccaggt
      961 aattcagtat ccgctcaatc accggttgct gttcgataca agtcaatgct ttcaccccga
     1021 caccacaggg gtggcagcct ttaacatcaa tatcgcccaa ggcgcctgca taaaggtggg
     1081 tgtccctttg atgttgatcg gtgatttcgc tctcgagcgc atcagcgtgg tccggtgggg
     1141 cgatgttttc gaacccgagg gaggcgctgg ggagtgcaag tgcagatgac cgacttccgt
     1201 gctgtcgaag aactgtcaat ttctaaaaat caacctaatc aatattttgc gttgacttca
     1261 ctggcgattc agaatgtgtc gctagcgctc tgctaccggt aataagcagc gggacaccgg
     1321 tttcggcgat ccgatttgca gcaaatcggc gcgccgaggt cgagctttgc aaatagtctc
     1381 tattaatcct tgcctgcacg cccgggttga tcttgtgaat acccggcctc atggctatga
     1441 tgcggtgtct gtaccgaagg attacgaggt tttgtcatga aagtttgtgt actcggcgcc
     1501 gccggcggca tcggccagcc gctgtcgctg ctcctcaagc tgcaactgcc cgccggtagc
     1561 gaattgtcgc tctacgacgt ggccccgttt acccccggcg tggcggtcga cctgagccat
     1621 attccgaccg acgtctgcgt caccggctat gttggcgacg acgtcgaggc ggcgatgaag
     1681 gatgccgata tcgtcatcat tccggctggc gtgccgcgca agccggggat gactcgcgac
     1741 gacctgttca acatgaatgc cggcattgtc aagaagctga tcggcgttgg cgccaaggcc
     1801 tgtcccggtg cgctgttcgg cattgtcacc aacccggtca actcgaccgt gccgatcgcc
     1861 gccgaggtgt tgaaggccgc cggcgtctac gacaagcgca agttgttcgg cgtcaccacg
     1921 ctcgacgtga tccgctccaa caccttcgtc gcgcaaaccg ccggcaagag cactggcgag
     1981 gtcaacgtca acgtcatcgg cggtcacagc ggcgttacca tcctgccgct gctgtccagc
     2041 gtacccggtg tgagcttaag tcaggccgat atcgaggcgc tgacaccgcg catccaggaa
     2101 gccggcaccg aggtggtcga agccaaggcc ggtgccggtt ccgcgaccct gtcgatggcg
     2161 caggcggcgg cgcgtttcac gatgtcgctg gtcgccgcgc tcggcggcga gagcgtgacc
     2221 gagtgctgct atgtgcaggt cggcgac
//
LOCUS       DATCZV010000063         7491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_481189, whole genome shotgun sequence.
ACCESSION   DATCZV010000063 DATCZV010000000
VERSION     DATCZV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481189"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VIW27_01430"
     CDS             <1..194
                     /locus_tag="VIW27_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738351.1"
                     /translation="EGGFLDRGPSVHTAREGSGEKSNAAVEPRSRREGGFLDRGPSVH
                     TAREGPGEKSNAAIAARSR"
     gene            complement(192..533)
                     /gene="rnpB"
                     /locus_tag="VIW27_01435"
     ncRNA           complement(192..533)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIW27_01435"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(586..897)
                     /locus_tag="VIW27_01440"
     CDS             complement(586..897)
                     /locus_tag="VIW27_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY5738352.1"
                     /translation="MIRVIIEREIAEGLEQYYHAAVSDLLKAMTDAPGYLSGESLVEI
                     SRPNHYVVLTRWASEEAWQRWFKSPQRQQFLDAIRPFMLNDEKFMLLRQRHYNDRANP
                     V"
     gene            1007..2830
                     /locus_tag="VIW27_01445"
     CDS             1007..2830
                     /locus_tag="VIW27_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5738353.1"
                     /translation="MTWQRQILRVDLSSGKCAIEPLNMDWADAYLGCRGLGTKYLYEE
                     MDPAADALSAANKIIFATGPMTGTMASTGGRYAVVTKGPLTGAIACSNSGGKFGAELK
                     MAGYDLLIIEGESQQPVYLHIEDDRVELCDASDTWGKTVWETEEILAARYRDPLMRFA
                     GIGQAGENLCRYACVVNDRHRAAGRSGVGAVMGSKKIKTIAVRGTLGVRASGDPKKYY
                     QVINEVNSRLDDREGMARDGTLAMMDVTNGFGALPTRNNRSVRFEGADKLNPEAMHTP
                     NENGHTNIITNGACFGCTIGCGRICHIDPTHFSVKDKPQYHGASGGLEYETAYALGAA
                     CGVDDIDAATYAGFLCNEYGMDPISLGGSIAAAMELYDIGAIDDEVTGGIKLEFGNAE
                     ALCAIAELAGKGEGFGADVAMGSKRLCEKYGHPDLSMTVKGQEFAAYDGRGMQGMGLA
                     YATSNRGACHLRADPYAHDFETTEIAGKAEIVAQTQRDTAFIDSSGLCLFPGGCGWGL
                     EDYRALVDAACEGDWSSEQRMQETGERIWNLERLFNLRAGLGKADDTLPKRILEEPAD
                     VGTAKGLVCKLDEMLPEYYQCCGWDESGVPTQATLSRLGLD"
     gene            2853..3104
                     /locus_tag="VIW27_01450"
     CDS             2853..3104
                     /locus_tag="VIW27_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5738354.1"
                     /translation="MQVAVTLFGGLRHFLPPGSSFNKCEIEVDDGARLDQLLERIPIP
                     ADKPYLVLFNDEKINAEDYPGIQVKPGDEIVLLPPIKGG"
     gene            complement(3244..4797)
                     /locus_tag="VIW27_01455"
     CDS             complement(3244..4797)
                     /locus_tag="VIW27_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY5738355.1"
                     /translation="MKKAAARKPATKKSAARKPASRKPISTGAEWTFELIEAYHQEIE
                     RIAQDFGLDTYPNQIELITAEQMLDAYASVGMPIGYSHWSFGKHFIRNEQQYRRGHMG
                     LAYEIVINSNPCIAYLMEENTMMMQALVIAHASFGHNSFFKGNYLFRAWTDAEAIIDY
                     LVFAKRYIAECEERYGIDEVENILDSCHALQNHGVDRYKRPNPVSMREEKQRLKEREA
                     YLQSQVNDLWNKTVPKKKAREEQEKQLNFPQEPQENILYFVEKNAPLLEPWQREVVRI
                     VRKIGQYFYPQRQTQVMNEGWACFWHYHIMNELYDRGKVTDGFMLEFIQSHTNVVAQP
                     DFDSDYYSGINPYALGYAMMMDIKRICVEPTEEDRKWFPDIAGSDWQQTLDFAMRNFK
                     DESFISQFLSPRLIREFKMFSILDDEAQSELEVTSIHNDAGYRHIRQSLSNQYNLSMN
                     EPNIQVYNVDVRGDRSLTLRHVPHNSVPLAETCEEVLKHLHRLWGFSVKLETLDEDGE
                     VVSTAECAA"
     gene            complement(4794..6059)
                     /locus_tag="VIW27_01460"
     CDS             complement(4794..6059)
                     /locus_tag="VIW27_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006086331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEY5738356.1"
                     /translation="MAQLIDRRPNAKGKSTVNRQRFLRRYKKQIKKAISDQISERSIT
                     DIEGGESVSIPTRDISEPTFRSDQGGVREGVFPGNKEFASGDRIKRPPGGGGGGQGRR
                     ASDSGEGEDDFSFEISRDEYLDMLFDDLELPNLQKNQLRRMTKSKTVRAGFTNDGVPA
                     NINIVRSLRSALSRRVAMGAEPRRMLREAEAALEALENDPTADEIKLLQLRQEVDHLR
                     ARLKRIPFIDTFDLRFNNYVQRPEPTSQAVMFCLMDVSGSMDQDTKDIAKRFYILLYL
                     FLSRSYRDVDVVFIRHHTQAKEVDEQEFFYSRETGGTIVSSALELTRDIIAQRYPPTD
                     WNIYAAQASDGDNWNDDSPICERILAKELLPQLRYYAYVEITRREHQGLWTHYENLLK
                     THDNFAMRHIREQQDIYPVFREFFRKHAQ"
     gene            complement(6076..>7491)
                     /locus_tag="VIW27_01465"
     CDS             complement(6076..>7491)
                     /locus_tag="VIW27_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY5738357.1"
                     /translation="KIMSPWAVKRLNEYGGDITQFKVVKVQPSILNQIAVAKTEPGDE
                     NNQDISSLVGKVDIRMLEHYAQNDADAYSYSGALCRANQGLMEFVEMFKAPIKVLHPL
                     LTATQEGNYNGTEGLSALPFEGTILAHSNESEWQSFRNNKNNEAFLDRVYIVKVPYCL
                     RVSEEIHIYEKLLQNSSLSAAPCAPSTLEMLAQFSVLSRLVMPENSSIYSKMRVYDGE
                     SLKDTDPKAKSYQEYRDYAGVDEGMRGLSTRFAFKILSRVFNFDQTEVAANPVHLLYV
                     LEQQIAREQFPQDVHDRYLEHIKAFLVPNYVEFIGKEIQTSYLESYSEYGQNIFDRYV
                     TYADFWIQDQEYRDPETGENFGRASLNEELEKIEKPAGISNPKDFRNEVVNFVLRARA
                     QNEGNNPNWVSYEKLRTVIEKKMFSNTEELLPVISFSAKASEEDEKKHANFVSRMVEK
                     GYTEKQVRLLCEWYLRVRKAS"
BASE COUNT         1403 a         2250 c         2294 g         1544 t
ORIGIN      
        1 gagaaggcgg atttctcgac cgaggcccga gcgtgcatac cgcacgtgag ggttcgggcg
       61 aaaaatccaa cgccgcagtc gagccgcgca gcaggcgaga aggcggattt ctcgaccgag
      121 gcccgagcgt gcataccgca cgtgagggtc cgggcgaaaa atccaacgcc gcaatcgcgg
      181 cgcgcagcag gtgagggagt cagcctgtaa gccgggttct gtcttggata atcattcatc
      241 tgggccaaga gtcacctctt agctcaagca accaacccga aaacggcgcg ggcaacgcca
      301 tggttttcct atttggtctt gctccgggtg gggtttaccc tgccacccct gttaccaggg
      361 gcgcggtgcg ctcttaccgc accatttcac ccttaccggc cgcagccggc ggtatacttt
      421 ctgttgcact ggccgtaggc tcgcgccccc cagacgttat ctggcaccct gccctgtgga
      481 gcccggactt tcctccagtt cccgagggaa ccagcgatta tccggctgac tcgctggcat
      541 tctaaccgcg cgcgcgacgc cgcgccagtc tcctggcatg gaccgctaga cagggttcgc
      601 cctatcgttg tagtgccgct gcctgagcag catgaatttc tcgtcgttga gcatgaaggg
      661 gcggatcgca tcgagaaatt gctggcgctg aggtgacttg aaccagcgtt gccaggcttc
      721 ctcgctggcc catcgcgtga gcaccacata gtgattgggt cggctgattt cgaccagcga
      781 ttccccggac aggtaaccgg gcgcgtcggt catggccttg agcaggtcgg agaccgccgc
      841 atggtagtac tgttccagcc cctcggcaat ttcacgctcg atgatgaccc tgatcatgaa
      901 tggtgtttcc tcgactttac cggcctcgca accgacaaga tgtgttaaaa gaaacccgca
      961 ggatatacaa tccggcttat aacacaaacc tcccggagaa taccgaatga cctggcaaag
     1021 acagattctg cgcgtcgatc tgagttcggg caagtgcgct atcgagccgc tgaacatgga
     1081 ctgggccgac gcctacctgg gctgtcgcgg cctcggcacc aagtaccttt acgaagagat
     1141 ggatccggcc gccgatgcac tgtcggccgc caacaaaatc atcttcgcca ccgggccgat
     1201 gaccggcacc atggcttcga ccggcgggcg ttacgcggtt gtcaccaagg gtccgctgac
     1261 cggcgccatc gcctgctcga attccggtgg caagttcggt gccgagctga agatggcggg
     1321 ttacgacctg ctgatcatcg agggcgaatc gcaacagccc gtctacctgc acatcgagga
     1381 cgacagggtc gaactctgcg acgctagtga tacctggggc aagacggtct gggagaccga
     1441 ggaaatccta gccgccaggt accgcgatcc gctgatgcgc ttcgccggca tcggacaggc
     1501 cggagaaaac ctgtgccgtt acgcctgcgt ggtcaacgac cggcaccgcg cggcgggccg
     1561 ttccggcgtc ggcgcggtga tgggatcgaa aaagatcaag actatcgcgg tacgcgggac
     1621 gctcggcgtg cgcgccagcg gcgatccgaa aaaatactac caggtcatca acgaggtcaa
     1681 ttcgcggctc gatgaccgcg agggcatggc gcgcgacggc acgctggcga tgatggacgt
     1741 caccaacggc ttcggcgccc tgccgacgcg caacaaccgc agcgtgcgct tcgagggcgc
     1801 cgacaagctc aatcccgagg cgatgcatac gccgaacgag aacgggcaca ccaatatcat
     1861 taccaacggc gcctgcttcg gctgcaccat cggctgcgga cgcatctgcc atatcgaccc
     1921 gacgcacttc tcggtcaagg acaagccgca ataccacggc gcttccggcg ggctcgagta
     1981 cgaaaccgcc tatgcgctcg gtgcggcctg cggggtcgac gacatcgacg ccgcgaccta
     2041 tgccggattc ctctgtaacg agtacggcat ggacccgatc tcgctcggcg gctcgatcgc
     2101 cgcggcgatg gagttgtacg acatcggcgc catcgacgac gaggtgaccg gcggcatcaa
     2161 gctcgagttc ggcaatgccg aggcgttgtg cgcgatcgcc gaactggccg gcaagggcga
     2221 aggtttcggc gccgacgtcg ccatgggttc gaaacgcctg tgcgaaaaat acggccaccc
     2281 cgacctgtcg atgacggtca agggccagga gtttgccgcc tacgacggcc gcgggatgca
     2341 gggcatgggc ctcgcctacg cgaccagcaa tcgcggcgcc tgccacctgc gagccgatcc
     2401 ctacgcgcac gacttcgaaa ccacggaaat cgccggcaag gccgagatcg tcgcgcagac
     2461 gcagcgcgac accgcgttca tcgattccag cggcctgtgc ctgttcccgg gtggttgcgg
     2521 ctggggcctg gaggactacc gcgcgctggt cgatgccgcc tgcgaaggcg actggagcag
     2581 cgagcaacgc atgcaggaaa ccggcgagcg catctggaac ctcgagcggc tgttcaacct
     2641 cagggccggt ctcggcaagg ccgacgacac cttgccgaag cgcattctcg aggagccggc
     2701 cgacgtcggc accgccaagg gcctggtctg caagctcgac gaaatgctgc ccgagtatta
     2761 ccagtgctgc ggctgggacg agagcggcgt gccgacccag gccacgctga gccggctggg
     2821 gctggactag ccgccggccg ggagccgctg ccatgcaagt cgcggtgacg ctgttcggcg
     2881 gcctgcgtca tttcctgccg cccggcagct cgttcaacaa gtgcgagatc gaggtcgatg
     2941 atggcgccag actcgatcaa ttgctcgaac ggattccgat acctgccgac aagccctacc
     3001 tggtgctgtt taacgacgaa aagatcaatg ccgaggacta tccggggatc caggtaaaac
     3061 ccggcgacga aatcgttctg ttaccgccga tcaagggcgg ctgaacggtc agccacggat
     3121 tcgttgtaac acggcgcgga tgtcacctcg ctgaacctcg gcgggcgttc actttgcaga
     3181 ccgatgtcgg gccggtatat gccgctctgc cagcggcctg cttcgaatca cccgacccgt
     3241 gggtcaggcc gcgcactcgg cggtgctgac gacttcgccg tcctcgtcga gggtctcgag
     3301 cttgacgctg aaaccccaca ggcggtgcag atgcttgagt acttcctcgc aggtttcggc
     3361 tagcggcacc gaattgtgcg gcacgtggcg cagtgtcagc gaacggtcgc cgcgcacgtc
     3421 gacgttgtaa acctggatat tgggctcatt catgctcagg ttgtactggt tcgacagcga
     3481 ctggcgaatg tgccggtagc cggcatcgtt gtggatcgaa gtcacttcga gctcgctttg
     3541 cgcctcgtcg tcgagaatcg agaacatctt gaactcgcga atcaggcgcg gcgacaggaa
     3601 ctgtgagata aaactctcgt ccttgaaatt gcgcatcgcg aagtcgagcg tttgctgcca
     3661 gtcggagccg gcaatatccg gaaaccattt gcgatcctct tccgtgggct cgacgcagat
     3721 gcgcttgata tccatcatca tcgcgtagcc gagcgcgtag ggattgatgc cgctgtaata
     3781 gtcgctgtcg aaatccggct gggcgaccac gttggtatga ctctggatga attcgagcat
     3841 gaaaccatcg gtcaccttgc cgcggtcgta gagctcgttc atgatgtggt aatgccagaa
     3901 gcacgcccag ccctcgttca ttacctgggt ctggcgctgc gggtaaaaat actggccgat
     3961 cttgcgcacg atgcgcacca cctcgcgctg ccacggctcc agtaacgggg cattcttttc
     4021 gacgaaataa aggatgtttt cctgcggctc ctgcggaaaa ttgagctgct tttcctgttc
     4081 ctcgcgcgcc ttctttttcg gcacggtctt gttccacagg tcgtttacct gggattgcag
     4141 gtaagcctcg cgctccttga gccgctgctt ttcctcgcgc atcgataccg ggttgggccg
     4201 cttgtagcga tccacgccgt gattttgcag cgcatggcag gaatcgagga tattctcgac
     4261 ctcgtcgatg ccgtagcgct cttcgcattc ggcgatatag cgcttggcga acaccaggta
     4321 gtcgatgatg gcttcggcgt cggtccaggc gcgaaacaga tagttgccct tgaaaaacga
     4381 gttgtgtccg aagctggcgt gggcgatgac cagcgcctgc atcatcatcg tattttcctc
     4441 catcaggtag gcaatgcagg gattggagtt gatgacgatc tcgtaggcga gccccatgtg
     4501 gccgcggcgg tactgctgct cgttacgaat gaagtgcttg ccgaaagacc agtgactata
     4561 gccgatcggc ataccgacgc tggcataggc gtcgagcatc tgctcggcgg tgatcagctc
     4621 gatctggttc ggataggtat cgaggccgaa atcctgcgcg atacgctcga tttcctggtg
     4681 gtaggcctcg atcagctcga aggtccactc ggcgccggtg gaaatgggct ttctcgaagc
     4741 cggctttctc gcggctgatt ttttcgtagc tggctttctc gcagccgcct ttttcattgc
     4801 gcgtgcttcc tgaagaactc gcggaacacc gggtagatgt cctgctgctc gcgaatgtga
     4861 cgcatggcga aattatcgtg ggttttcaac aggttttcgt aatgcgtcca cagcccctgg
     4921 tgctcgcgcc gggtgatctc gacgtaggcg taataacgca gctgcggcaa cagctccttt
     4981 gccaggatgc gctcgcagat cggcgagtcg tcgttccagt tgtcgccgtc cgaggcctgc
     5041 gccgcgtaga tattccagtc ggtgggcgga tagcgctggg cgatgatgtc gcgggtcagc
     5101 tcgagcgcgc tggagactat agtaccgccg gtttcgcgcg aatagaaaaa ctcttgctcg
     5161 tcaacttcct tggcctgggt gtggtggcga ataaagacca cgtcgacgtc gcggtagctg
     5221 cggctgagaa acaggtagag caggatatag aagcgcttgg cgatatcctt ggtatcctgg
     5281 tccatcgatc ccgacacgtc catcaggcaa aacatcaccg cctgcgaggt cggctcgggc
     5341 cgctgcacgt agttgttgaa gcgcaggtcg aaggtatcga tgaagggaat gcgcttgagg
     5401 cgcgcacgca ggtgatcgac ttcctggcgc agctgcagca gcttgatctc gtcggcggtg
     5461 ggatcatttt cgagcgcctc gagtgccgcc tcggcctcgc gcaacatgcg gcgcggttcg
     5521 gcacccatcg ccacgcgccg cgacagtgcc gagcgcagcg aacgcacgat gttgatgttg
     5581 gcgggcacgc cgtcgttggt gaagccggca cgcacggtct tgctcttggt cattcggcgc
     5641 agctggttct tctgcaggtt cggcaattcg agatcgtcga acagcatatc gaggtactcg
     5701 tccctggaaa tctcgaaact gaaatcgtcc tcgccctcgc cgctatcgct ggcgcgccgg
     5761 ccctggccgc ccccgccgcc gccggggggc cgcttgatgc gatcgccgct ggcgaattcc
     5821 ttgttgcccg ggaacacgcc ctcgcgaacc ccgccctggt cactgcgaaa ggtcggctcg
     5881 ctgatatcgc gcgtcgggat ggacacgctt tcgccgccct cgatgtcggt gatcgagcgc
     5941 tccgaaatct gatccgagat agccttcttg atctgcttct tgtaacgccg caggaagcgc
     6001 tgccggttta ccgtgctctt gcccttggcg ttgggccgtc tatcgatcag ttgcgccatc
     6061 gtcgagttcg ctccgtcagg acgccttcct gacccgcagg taccattcgc aaagcaggcg
     6121 cacctgcttt tcggtgtagc ccttttcgac catccgcgag acgaagttgg cgtgtttctt
     6181 ttcgtcttcc tcggaagcct tggcgctgaa cgaaatcacc ggcagcaatt cctcggtgtt
     6241 ggagaacatc tttttttcga tcaccgtgcg caacttctcg taactgaccc agttcgggtt
     6301 attgccttca ttttgcgccc gcgcccttaa cacgaaatta accacctcgt tgcgaaagtc
     6361 cttgggattc gagattccgg ccggtttctc gatcttttcg agttcttcgt tgagcgacgc
     6421 gcggccgaag ttctcgccgg tctccgggtc gcggtattcc tggtcctgga tccagaaatc
     6481 ggcataggtg acatagcggt cgaagatgtt ctggccgtac tcggaatagg actcgaggta
     6541 tgaagtctgg atttccttgc cgatgaattc gacgtagttg ggcaccagga acgccttgat
     6601 gtgctcgagg taacggtcgt gcacgtcctg cgggaactgc tcgcgcgcga tctgctgctc
     6661 gagtacgtag agcaggtgca ccgggttggc ggcgacctcg gtctggtcga aattgaacac
     6721 tcgcgacagg atcttgaacg cgaaacgcgt cgacaggccc cgcatgccct cgtcgacgcc
     6781 ggcatagtcg cgatactcct ggtaggactt ggccttgggg tcggtatcct tgaggctttc
     6841 gccgtcgtag acccgcatct tcgagtagat gctcgagttc tccggcatca ccagccgcga
     6901 cagcacgctg aactgcgcca gcatctcgag cgtactcggc gcgcacggcg ccgccgacag
     6961 cgaactgttt tgcaacagct tttcataaat gtggatttcc tcggacaccc gcaggcaata
     7021 cggcaccttg acgatgtaaa cccgatcgag aaaagcctcg ttgttcttgt tgttgcggaa
     7081 cgactgccac tcggactcgt tggagtgcgc gagaatggtg ccctcgaagg gcagtgccga
     7141 gaggccctcg gtaccgttgt agttaccttc ctgggtcgcg gtcagcagcg ggtgcagtac
     7201 cttgatcggc gccttgaaca tttcgacgaa ttccatcaga ccctggttgg cgcggcacag
     7261 cgcgcccgag taactgtagg catcggcatc gttttgcgcg taatgctcga gcatgcgaat
     7321 gtcgaccttg ccaaccagcg aggagatatc ctggttgttc tcgtcgccgg gctcggtctt
     7381 ggcgaccgcg atctggttga gaatcgaagg ctgcaccttg accaccttga actgcgtgat
     7441 atcaccgccg tattcgttga ggcgcttgac cgcccagggt gacatgatct t
//
LOCUS       DATCZV010000064         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_269545, whole genome shotgun sequence.
ACCESSION   DATCZV010000064 DATCZV010000000
VERSION     DATCZV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269545"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..817)
                     /locus_tag="VIW27_01470"
     CDS             complement(110..817)
                     /locus_tag="VIW27_01470"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEY5738358.1"
                     /translation="MSREHRSGNCHILQRQDRRQVLEAEQFTAGFWHGGQTFQNQVGG
                     RGDNCLIDVDGTQAVLRQYRRGGFVGHLLSNQYLWLGWRLSRPWREWQVLERARDAGL
                     PVPEPIAACACRSYFWYRAALMTSYLEDTEMLTKRLEREALDPGLWRSLGQLIKRMHD
                     ERIRHADLTSDNILIDSANRFYLIDFDQARIMKRLDDWQWQPLYRFQRSMIKRNRRRP
                     LHFGAAEWQALMDGYQA"
     gene            complement(801..2135)
                     /gene="mtaB"
                     /locus_tag="VIW27_01475"
     CDS             complement(801..2135)
                     /gene="mtaB"
                     /locus_tag="VIW27_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY5738359.1"
                     /translation="MLVNFQALGCRLNEAEVETWASQFLQRGYQLTAEAAEADVVVFN
                     SCAVTARADRKSRQQIGRLQRSNPQARLVVTGCHASLNREQVKRYLGVDLVVDNRDKD
                     RLVEQTLELLGAGSGTAVAPPTLETAPNALLERGRHRAFVKVQDGCRYRCTYCVVTLA
                     RGEERSRGEAEILAEINRLHAQGIQEVALTGVHVGGYGSDTGSSLYRLLAEILEHTAM
                     PRIRLASVEPWDLPAEFFGLFKDSRLMPHMHLPLQSGCDSVLRRMARRCKTAEFAALV
                     ERARDAVPRFNITTDLIVGFPGESEQEWRQTMRYVADIGFGHMHIFPFSPRAGTKAAR
                     LDEQVDAQTKKLRGEEMQSLAVELKRRALREHLGGRAEVLWERRLAGGTGDWLGYTPH
                     YHKIRSANAAIEAARILEVGIDAISGDGSMLVDAAARAQVALTDFGFHVAGT"
     gene            complement(2327..>2679)
                     /locus_tag="VIW27_01480"
     CDS             complement(2327..>2679)
                     /locus_tag="VIW27_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738360.1"
                     /translation="LIYRRLLDIFPGDYVLSMRLLRLLKQTGRNQSALVIARQLEIDF
                     PQRQQIYFELSEIYQSLDRPIMKLMAEAEFHRITGNIKQAIRLYDQVLKSADADIATR
                     SRAREKRLVLLEEK"
BASE COUNT          510 a          880 c          803 g          486 t
ORIGIN      
        1 ggcgcgaggc gcgcgctcgg ctcgccgcgc agcccgacgt ggtcgaacaa tacctggccg
       61 agatcaggcc ttacctgtag cgcgggcagg tagcacggca ggcggccgct caagcctggt
      121 agccatccat cagcgcctgc cattccgcgg cgccgaaatg caacggccgg cgccgattac
      181 gcttgatcat cgaacgctga aagcggtaca gcggctgcca ctgccagtca tcgagccgtt
      241 tcatgatgcg cgcctggtcg aaatcgatca ggtaaaagcg attcgcggaa tcgatcagaa
      301 tattgtcgga agtcaggtcg gcatggcgaa tgcgctcatc atgcatgcgc ttgatcaact
      361 gacccagcga ccgccacaaa ccgggatcaa gcgcttcgcg ctccagccgc ttggtcaaca
      421 tttcggtatc ctcgagatag ctggtcatca gcgcggcgcg gtaccagaaa taactgcggc
      481 aggcacaggc ggcgatcggt tcgggtaccg gcaacccggc gtcgcgcgcc cgttcgagca
      541 cctgccattc acgccacggc cgcgatagtc gccagcccag ccacaggtat tgattgctca
      601 gcaggtgacc gacgaaaccg ccgcggcggt actgccgcag caccgcctga gtgccgtcga
      661 cgtcgatcag gcaattatcg ccacgcccgc cgacctggtt ctgaaacgtc tgcccgccgt
      721 gccagaagcc ggctgtgaat tgctccgcct cgagcacttg ccgacgatcc tgccgctgca
      781 ggatatggca gtttcccgac ctatgttccc gcgacatgaa aaccaaagtc agtcagtgcg
      841 acctgcgctc gagcagccgc gtcgaccagc atcgaaccgt cgccggaaat cgcatcgata
      901 ccgacctcga gaattcgcgc cgcttcgata gccgcgtttg ccgagcgaat cttgtggtaa
      961 tgcggggtat acccgagcca gtctccggta ccgccggcga ggcggcgttc ccacaagacc
     1021 tcggcgcgtc cacccaggtg ctcacgcaat gcccgccgct tgagttcgac cgccagagac
     1081 tgcatttcct cgccgcgcaa cttcttggtc tgcgcgtcga cctgctcgtc gagccttgcg
     1141 gccttggtgc cggcgcgcgg cgagaacggg aatatgtgca tgtgcccgaa gccgatatcg
     1201 gcgacgtaac gcatcgtctg ccgccactcc tgttcacttt ctcccggaaa cccgacgatc
     1261 aggtcggtgg tgatattgaa tcgcggcacc gcgtcgcgag cacgctcgac gagcgccgcg
     1321 aactcggccg tcttgcagcg ccgggccatg cgtcgcaata ccgaatcgca accgctttgc
     1381 agcggcagat gcatgtgcgg catcagccgg gaatccttga acaggccgaa aaattcggcg
     1441 ggcagatccc agggctccac cgacgccagg cgaatgcgcg gcatcgcggt atgctcgagg
     1501 atttcagcga gcaagcggta caggctggag ccggtatcgc tgccgtagcc gccgacatgc
     1561 accccggtga gcgcaacttc ctggatgccc tgggcatgca ggcgattgat ctcggcgaga
     1621 atctcggcct cgccgcggct gcgctcctcg cccctcgcca gcgtcaccac gcaataggta
     1681 cagcgatagc ggcagccgtc ctggaccttg acgaaagcgc gatgccggcc gcgctccagc
     1741 aacgcgttag gcgcggtttc cagtgtcggc ggagccaccg ctgtaccgct gcctgcgccc
     1801 aacaactcca gggtttgctc gaccagccga tccttgtcgc gattgtcgac caccaggtcg
     1861 acgccgaggt atcgcttcac ctgctcgcgg ttgaggctgg cgtgacagcc ggtcaccacc
     1921 agccgcgctt gcggattact gcgctgcagg cgtccgatct gctgccggga tttgcggtcg
     1981 gcgcgcgcgg tgaccgcgca ggaattgaac accactacgt cggcttcggc cgcttccgcg
     2041 gtcaactggt agccgcgttg cagaaactgg ctggcccagg tttccacctc cgcttcgttg
     2101 aggcggcaac ccagtgcctg gaaattgact aacaccgctc ggtcgcttca caaaaagttc
     2161 gcggattcta acaagataac ccgctgcttg cagcggattc accgcaaaaa gtcatcaccg
     2221 cgcaagcggg gacctcgaaa agacggcgcg agataagatt cccgccgggc ggcgttccgc
     2281 ttgcgcggga atgattccag tattagcttg cctgtccggc cgcgggctat ttctcttcca
     2341 gcagcaccag ccgcttctcg cgtgccctgg aacgcgtcgc gatatccgcg tccgcggatt
     2401 tcagcacctg gtcgtaaagt cttatcgcct gcttgatatt gccggtgatg cggtgaaact
     2461 cggcctcggc catcaacttc atgatcggcc ggtcgagcga ctggtaaatc tcggacaatt
     2521 cgaagtagat ctgctggcgc tgcggaaaat cgatttccag ttgccgggcg atgaccagcg
     2581 ccgactgatt gcgtccggtc tgcttgagca ggcgcaacag gcgcatcgac agcacgtaat
     2641 cccccggaaa gatatccagc aggcgccggt aaatcagct
//
LOCUS       DATCZV010000065         8869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_428638, whole genome shotgun sequence.
ACCESSION   DATCZV010000065 DATCZV010000000
VERSION     DATCZV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428638"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VIW27_01485"
     CDS             complement(<1..1547)
                     /locus_tag="VIW27_01485"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY5738361.1"
                     /translation="MNSRFSEKWLATLCSLLPGVHSAVFMVPDPGNDRLHLLAHWPES
                     LEKYQDFFATVKYALKKRDEVCLARAHVIDGEALDFFAKPVYIRSTLAGVLAIKIKHL
                     PSNKHVAVFKSLKRSIRWLGLASFEKSDSDEFYGSVVGVLASCFEQDNYQQGLMRMVA
                     ELTSEYRCESVAFGEKHGHHCNVIAISNSAEFDQRSNLVRRIGDAMDEAIEQDSPVLF
                     PQLNSRMIVRAHQRLARKHDMNSVATIPLVAERKVFGAVTLVGSEKSPLDRKTIDKCR
                     QTLSLLTPFLMLKRDQERSILAKLAASIGDASRAVLGVRYLRTKLATIALVTLLTAAS
                     LVETDFRITADAVLEGEVQRVVAAPIAGYLLSSAVRAGDTIREGEIMATLNDEQLRLE
                     LARLAGRMQQARREYREAQSTRDLVGVRVIRGQINQISAEIELVEEQLESINLTAPFD
                     GVVIEGDLSQVLGSPVERGETLFKIAPLEDYRIIVKVDESEIAYIEPGQRGSLALSSL
                     SEQQLPIT"
     gene            complement(2215..3834)
                     /locus_tag="VIW27_01490"
     CDS             complement(2215..3834)
                     /locus_tag="VIW27_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5738362.1"
                     /translation="MTKPTTPTTSTRRAGSRFRSAAPTTRPPTRSASACRTSTRDEVM
                     KTQTHKLTILFATLLLSACGADISDSLDDSADAGSADFTTFVAVGDSLTAGYADGALY
                     RHGQENSFPAILAQQFALAGGGAFSQPLMPVGATGSLTHSAADLGRADRLVLVPTVNP
                     SQPAAPGTVTPVQPTAIDVRVGSGGFNNMGIPGAKAIHLNVSAYGENSLAAFVGGTAN
                     PYFARFSSNDTTTVMTDFFGQAPTFFILWIGSNDVLLYAVDGGPGGTPAYGTGTTDVT
                     DPALFEAAYNGTLAAIATNLPTAKGVLVTVPDVGTIPYFTTVPFNPIPMDAATAAGAN
                     AAYQTYNDGLLAAEAGLIIDADERQQRTINFSAGQNALVILDDSLTPIPGAPLLRQAT
                     ANDLIILTASTKIGTPSDLSNPLTTIWGVGTPMEDADVLTETEYAFVQAARDEYNADI
                     VAAANGNPNFVLFDAATLLEELNETGILYGSGGISATFAQGGGISLDGIHPTARGYAV
                     MANELMKVIENAFGAKLPPVDPNQYTTVFYQ"
     gene            complement(3716..4963)
                     /locus_tag="VIW27_01495"
     CDS             complement(3716..4963)
                     /locus_tag="VIW27_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HEY5738363.1"
                     /translation="MKKTIMALVCLMTAGSLQAGGYRVATQGQKALAMGHAGVAMSDS
                     AETVFFNPGAITQLEANADFTGGLTLLDGETQYQQQATLVEAETDNPLGTPINAYYAR
                     KYSDKVSWGLGIYTPFGNKVKWPTDWAGSHLVNEIELQSIFIQPTIAYQINDSTSIGF
                     GPNLIIGAVEFNRNLSTSLTNADGDRSNVTIKEDNVTDIGFSLGLLHKFNQQTSLGVS
                     YRSEVILEARGGDATFDNIPTALQPIFTDTKFDADLPLPAELTVGIAHRFSDQLTLAF
                     DINRTFWDVYEELVVEFDNNVPTSVNPRNYEDANIYRIGLQYQYDDKWTLRGGIYFDE
                     SPVPDGSFAPETPRNDSIGYTAGFTYRYSQNLELDFSLLILTFDETDNSYDFYEEGGV
                     TIPFSGTYDSAANSIGFGLSYKY"
     gene            5120..5419
                     /locus_tag="VIW27_01500"
     CDS             5120..5419
                     /locus_tag="VIW27_01500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY5738364.1"
                     /translation="MQTDTFVADSDIHGKGLFAARRIPAGTTIGQLRVRPAETDGSHV
                     LWLSETEGVEVICDLKYINHCERPNACYYDDLSVVALRDIEAGEEITHDYCCGDW"
     gene            5479..6102
                     /locus_tag="VIW27_01505"
     CDS             5479..6102
                     /locus_tag="VIW27_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY5738365.1"
                     /translation="MTTTAAKFNMIEQQIRPWEVLDPRVLAVIDEVSREDFVREPYRG
                     LAYADCQIPVADGVRMLPPTVEGRLLQSLNIEPGDEVLEVGSGCGYLAACLAKLGARV
                     TSIDTSAEVIEFAAANIAAAGIDHVEFRQLSAAEFDAPQSCDAIAVTASLPKVPDNLR
                     QALRTGGRLFVIVGQSPVMEALLITRASESEWLTRSLFETDLPRLRV"
     gene            6112..6426
                     /locus_tag="VIW27_01510"
     CDS             6112..6426
                     /locus_tag="VIW27_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5738366.1"
                     /translation="MQRMNAPQLAAYLADADPLLLDVREAWEFEICHIDGSINLPMSQ
                     IPAHLEQLEGADEIVLICHHGIRSLRVAGYLQQALDATLINLDGGVESWAREVDPEMA
                     VY"
     gene            6480..7793
                     /locus_tag="VIW27_01515"
     CDS             6480..7793
                     /locus_tag="VIW27_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY5738367.1"
                     /translation="MPKLRSITTLLALLGSWAPLHAQDLLTVYELALENDAQLQIAEA
                     NYLAAIEALPQARSGRKPQVFFNADATVVESDNSRLGDDSSDSVGYGVSLSQALYDTE
                     TRGNVLSAEASTRAELARLQSVRQAVALRVAEAYFAILAAHDNVDFAYAERTAIERQL
                     EQAQKRFEVGLIAITDVQEAKARFDSAEAQAILAENILENAYQALVVITGDPSVRNLW
                     RLESQLALTLPDPADANAWVEVALQNNRDLIAAREDLNAARFERDKRNRRGYPTLDLV
                     ASYEDRDTDSDLLGDTQQEDVTVGIQLEIPLYTGGRISSEQAEAEAQLVSAQNNTLLQ
                     TRLASQRSRIAYLDVVSGISQVSALEQALESSNVALEATQAGFEVGTRTSVDVLVSLR
                     ETYRAQRDYASSRYEFLLNKLRLKQAAGTLAQEDLSEINRRLIRP"
     gene            7797..8726
                     /locus_tag="VIW27_01520"
     CDS             7797..8726
                     /locus_tag="VIW27_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEY5738368.1"
                     /translation="MGADSSVTFRARDYLSPRFLPMWVTIGLLRLVALLPYPAAMAAG
                     RLLGRLIYLIPGSRRKVVDTNLRHCFPQKSAAERERIKRACYQNIGVSIIEMALCWWW
                     PDRRLEPLVELEGREYLDAVLDSGRGAILLSGHFTSLEIGGRLLSLYLPFQAMYRTQR
                     NALFDSYLYTRRSSYLADVVSRKDTRKLIRGIRDKIPTWYAPDQDFRRERNVFAPFLG
                     VPAATITAGSRLAQTTGAAMLPYYPERKLDGSGYVLQIGPPLENFPSGDDVEDATAIN
                     RSIGDFVRLHPENYMWIHQRFKTRPPGEPRFYD"
BASE COUNT         1678 a         2754 c         2708 g         1729 t
ORIGIN      
        1 gtgatcggca attgctgctc ggagagactc gacagggcca gcgaaccccg ctgtccgggc
       61 tcgatatagg cgatctcgct ctcatcgacc ttgacgatta tgcggtagtc ttccagcggc
      121 gcgatcttga acagcgtttc accccgctcg accggcgatc ccagcacctg gctgaggtca
      181 ccctcgatca ccacgccgtc gaacggcgcc gtcaggttga tgctttccag ctgctcctcg
      241 accagctcga tttcggcgct gatctggttg atctgcccgc gaatcacacg cacaccgacc
      301 agatcgcgcg tcgattgcgc ctcgcgatac tcgcggcgcg cctgctgcat gcggccggcg
      361 agcctcgcca gctcgagcct gagctgctcg tcgttgaggg tcgccatgat ctcgccctcg
      421 cgaatcgtgt cgccggcgcg caccgccgac gacagcaggt atcccgcaat cggcgcggcg
      481 actacccgct gcacttcgcc ttcgagcacc gcatccgcgg tgatcctgaa atcggtttcg
      541 accaggctgg ccgccgtcag cagcgtgacc agcgcgatgg tcgccagctt ggtgcgcagg
      601 tagcgcacgc cgagcaccgc gcgcgaggca tcgccgatgc tggcggcgag cttggcgagg
      661 atgctgcgct cctggtcgcg cttgagcatc aggaacgggg tcagcaacga cagggtctgc
      721 cggcacttgt cgatggtttt gcgatcgagc ggcgatttct cgctgccgac cagggtgacg
      781 gcaccgaata ccttgcgctc ggcgaccagc ggtatggtcg ccaccgagtt catgtcatgc
      841 ttgcgggcca gtcgctgatg ggcgcgcacg atcatgcgac tgttcaactg cgggaagagc
      901 accgggctgt cctgctcgat cgcctcgtcc atcgcgtcgc cgatgcggcg caccaggttg
      961 gagcgctgat cgaattcggc gctgttggaa atcgcgatga cgttgcagtg gtgaccgtgc
     1021 ttctcgccga acgccacgct ctcgcagcgg tactcgctgg tcagctcggc aaccatccgc
     1081 atcaggccct gctggtaatt atcctgctcg aagcagctcg ccagcacacc gaccacgctg
     1141 ccgtaaaatt cgtcgctgtc cgacttctcg aagctggcaa ggccgagcca gcggatactg
     1201 cgcttgagcg acttgaacac ggcgacatgc ttgttgctcg gcaaatgctt tatcttgatt
     1261 gccagcacac ccgccagggt cgagcggatg tataccggct tggcgaaaaa atccagcgcc
     1321 tcgccatcga tcacgtgcgc gcgggccagg cagacttcgt cacgcttctt gagcgcatat
     1381 ttaaccgtgg cgaagaagtc ctggtatttc tccaggcttt ccggccagtg cgccagcagg
     1441 tgcagccggt cattgccggg atcgggcacc atgaaaaccg ccgagtgcac gccgggcaag
     1501 aggctgcaaa gcgtggccag ccatttttcc gagaagcgac tgttcacgcg ggtttaacct
     1561 cggcttgccg gacgggggcg atgtccggca attggcaggg acgcaagact ggccgcggga
     1621 gggtcggcat ttggcaggaa gggcggtggg aaaacgggcg caacacacgg gcaaccgcga
     1681 gcgccaggcg cacaacaggg cgcgcgatga ctgcctcgcg gtattcgatc gagcctggcg
     1741 ggtatgcgag gcataccggc gctgctcgga gattgtgctt caactgcctg ctcatttacg
     1801 cccctcaaaa cttccgcggt cgactcgcgg cctgttttcg tcgattcacg gcactcccgg
     1861 tcccacatgg cgagcatggt ttcgggcttg aagtgctgcg catccttaat aatgagtata
     1921 ggtgagtttc aaccctttcc tgccgccggg ggcgggacgg caagactaaa tccttatttt
     1981 gtgacgcatg cacgtgccgt ggcggtataa ccctttgaat tttcagttat ttaacgcctt
     2041 ttaccgttgc cagccgcatg ggcgcctgtt atcgccaccc ggcctgagta tagacattga
     2101 cgcggttttg tccggggaaa ttgagctggc ggcaaagctg gcaaaagcgg ccgaccgggt
     2161 tgccgtattc ggcaacccgg cagcaggcgt cggtgagtgc tccgcgggcg cggattactg
     2221 gtagaaaacg gtggtgtact ggttgggatc gaccggcggc agcttggcgc cgaaagcgtt
     2281 ttcgatcacc ttcatcagtt cgttggccat aaccgcgtaa ccgcgggcgg tcggatggat
     2341 accatcgaga gaaatcccgc cgccctgggc aaaggtggcg gatatgccgc cggagccata
     2401 gagaatgcca gtctcgttga gttcctcgag caacgtcgcg gcatcgaaca atacaaagtt
     2461 gggattcccg ttggcggccg ccacgatgtc tgcattgtat tcatcgcggg ctgcctgcac
     2521 gaatgcatat tccgtttcgg taagcacatc ggcatcttcc atcggcgttc ccactcccca
     2581 gatggtagtt agcggattcg acagatcgga aggcgttcct attttcgtag acgcggtcag
     2641 aatgatcagg tcgttggcgg ttgcctggcg cagcaaaggg gccccgggta ttggggtcag
     2701 gctgtcgtcg agtattacca gcgcattctg gcccgcggaa aaattgatcg tccgctgctg
     2761 gcgttcatcg gcatcgataa tcaagccggc ctcggccgcc agcaggccgt cattataagt
     2821 ctggtatgcg gcattggccc ccgccgccgt ggcagcatcc atcgggatcg ggttaaaggg
     2881 taccgtggtg aaatacggaa tggtaccgac atccggcacc gtaaccagca cccctttggc
     2941 ggtgggcagg ttggtcgcaa tcgcggccag ggtgccgttg taagccgctt cgaataatgc
     3001 cggatcggtt acatcggtgg tcccggtgcc atacgccgga gtgcctcctg ggccgccgtc
     3061 caccgcataa agcagaacgt cgttactacc gatccagagg ataaaaaacg tcggtgcctg
     3121 gccaaaaaag tccgtcatca cggtcgtcgt atcattagac gaaaaacggg caaagtacgg
     3181 gttagcggtt ccgccgacaa aagcggccag cgaattctcg ccgtaagcgg acacattgag
     3241 atggatcgcc ttggcaccgg gtatacccat gttattgaac ccgccggaac ctaccctgac
     3301 gtcgatcgcg gttggctgga ccggcgtgac ggttcccggg gcggcgggct gcgacggatt
     3361 tacggtgggc accagcacca ggcgatcggc gcgccccagg tcggcagcgg agtgggtcag
     3421 gctgccggta gccccgacgg gcatcagcgg ttggctgaat gcgccgcccc cggcgagtgc
     3481 gaattgctgc gccaggatcg cgggaaacga attttcctgc ccgtgccgat acagggcgcc
     3541 gtcggcataa cccgcggtca gcgaatcgcc gaccgcgaca aaggtcgtga agtccgcgct
     3601 gcccgcgtcg gcggagtcat cgaggctgtc ggagatatcc gcgccgcagg ccgacagcaa
     3661 cagcgtggcg aacaggatcg tcagtttgtg agtttgcgtc ttcattactt cgtccctagt
     3721 acttgtacga caggccgaag ccgatcgagt tggcggccga gtcgtaggtg ccgctgaacg
     3781 gaatcgtgac cccgccctcc tcgtagaagt cgtaggagtt gtcggtttcg tcaaaggtca
     3841 ggatcagcag ggaaaaatcc agttccaggt tctgcgaata gcggtaagtg aagcccgcgg
     3901 tatagccgat cgaatcgttg cgcggcgtct ccggcgcgaa cgagccgtcc gggaccggcg
     3961 attcgtcgaa ataaatgccg ccgcgcagag tccacttgtc gtcgtattga tactgcaggc
     4021 cgatgcggta gatgttggca tcctcgtaat tgcgcgggtt gacactggtc gggacgttat
     4081 tgtcgaattc gaccaccagc tcttcgtaga cgtcccagaa ggtccggttg atatcaaagg
     4141 caagggtcag ctggtcgctg aagcgatggg cgatgccgac cgtcagttcg gccggcagcg
     4201 gcaggtcggc atcgaacttg gtatcggtga atatcggttg cagcgcggtc ggaatattgt
     4261 cgaaggtcgc atcgccaccg cgcgcctcga ggatgacttc cgagcgatag ctgacgccga
     4321 gtgaagtctg ctgattgaat ttgtgcagca ggccgaggct gaagccgatg tcagttacgt
     4381 tgtcctcctt gatggtgacg ttggagcggt cgccatcggc gttggtcagc gaagtgctca
     4441 ggttacgatt gaactcgact gcgccgatga tcaggttggg gccaaagccg atgctggtcg
     4501 aatcgttgat ctggtaggcg atggtcggct gaatgaaaat cgactgcagc tcgatttcat
     4561 tgaccaggtg tgaacctgcc cagtcggtcg gccatttgac cttgttgccg aacggcgtgt
     4621 atatacccag cccccaggag accttgtcgg aatacttgcg cgcatagtag gcgttgatcg
     4681 gcgtgccgag tggattgtcg gtctctgcct cgaccagcgt cgcctgctgc tgatactggg
     4741 tctcgccgtc cagcagagtc aggccgccgg tgaagtccgc gttcgcctcc agctgggtta
     4801 tcgctccggg attgaagaat accgtttccg cgctgtcgct catcgcgacc ccggcatgcc
     4861 ccatggccag tgccttttgc ccctgggtcg cgacccggta tcccccggcc tgcagactgc
     4921 ccgccgtcat caggcaaacc agtgccatta ttgtcttttt catatggcta ccaaatcccc
     4981 ctctatagtt atgacttcgt cgggtcaggt cgctctgcgc agactgcttg agatcgattt
     5041 cctgccctga aattgttatt gtcactgcct aattagttgc aaaatgatac tcccaacctc
     5101 acgaaattca aaactctaaa tgcagacaga tactttcgtc gccgatagcg atattcacgg
     5161 caagggactg ttcgccgctc gccgcatacc ggcgggaacc accataggtc agctgcgggt
     5221 gcggcccgcc gagaccgatg gcagccacgt gctgtggctc agcgaaaccg agggtgtcga
     5281 ggtgatctgc gacctgaaat acatcaacca ctgcgagcgg ccgaacgctt gctactacga
     5341 cgacctgagc gtggtcgcgt tacgcgatat cgaagccggc gaggaaatca cccacgatta
     5401 ttgctgcggc gactggtgaa tacgaccgcc acagtgctat attatgcgcg cctgttaccg
     5461 agtttacggg aaccctgaat gaccacgaca gccgccaagt tcaatatgat cgagcaacag
     5521 atccgtccct gggaagtgct cgatcctcgt gtgctcgccg taatcgacga ggtcagtcgc
     5581 gaagacttcg tgcgcgaacc ctaccgtggg ctagcctacg ccgattgcca gattccggtg
     5641 gccgacggcg tgcgtatgct gccgccgacg gtcgagggcc gcctgctgca atcactgaac
     5701 atcgagcccg gtgacgaagt cctcgaagtt ggcagcggtt gcggttatct cgccgcctgc
     5761 ctggcaaaac tgggggcacg ggtgaccagc atcgacacca gcgccgaggt gatcgagttc
     5821 gccgccgcca atattgccgc cgccggcatc gaccatgtcg agtttcgcca gctttccgcg
     5881 gccgaattcg atgccccgca atcctgcgac gcgatcgcgg ttaccgcctc gttgccgaaa
     5941 gtgcccgaca acctgcgaca ggcgctgcgc actggcggcc gcctgttcgt catcgtcggc
     6001 cagtcaccgg tgatggaagc gctgctaata acccgcgcct ccgagtccga gtggctgacc
     6061 cgctccctgt tcgaaaccga tctgccgcgc ctgcgggtct aggcgcgcgc ggtgcagcgc
     6121 atgaatgcgc cgcaactggc ggcttacctg gctgacgcgg atccgctgtt gctcgacgtg
     6181 cgcgaagcct gggaattcga gatctgccat atcgatggca gcatcaacct gccgatgtcg
     6241 cagattcccg cgcacctcga acaactcgag ggcgccgacg aaatcgtcct catctgtcat
     6301 cacggcattc gcagtctgcg ggtggccggc tacctgcagc aggcgctcga cgccacgctg
     6361 atcaacctcg atggcggtgt cgagtcctgg gcgcgcgaag tcgaccccga aatggctgtg
     6421 tattaatcgc cccgcagttt atttataatc cgcccaccga ataagagatg atacccggaa
     6481 tgcctaaact gcgatcgatt actactttgc tggcgctgct cggaagctgg gcgcctctcc
     6541 acgcccagga cttgctgacg gtgtacgaac tggctctcga aaacgatgcc caactgcaaa
     6601 ttgccgaggc caattacctg gccgcgatcg aggccctgcc acaggcgcga tccgggcgca
     6661 agccgcaggt attcttcaac gccgacgcca ccgtggtcga atccgataat tcccgcctcg
     6721 gcgacgacag cagcgactcc gtcggctacg gcgtgagcct gagccaggca ctctacgata
     6781 ccgagacccg cggcaatgtg ctctctgcgg aagccagcac ccgcgccgag ctggcgcgcc
     6841 tgcagtcggt gcggcaggca gtggcactgc gcgtcgcgga agcttacttc gcgattctgg
     6901 cggcgcacga caacgtcgac ttcgcctacg ccgaacggac cgccatcgag cgccagctcg
     6961 agcaggcaca gaagcgtttc gaggtcggac tgatcgcgat caccgacgtg caggaggcca
     7021 aggcgcgctt cgacagcgcc gaggcgcagg cgattctcgc cgaaaacatc ctcgagaacg
     7081 cttaccaggc gctggtcgtc atcaccggcg atccgtcggt cagaaacctc tggcggctcg
     7141 aaagccagct ggcgctgacg ctacccgacc cggccgatgc caatgcctgg gtcgaggtgg
     7201 cattgcaaaa caaccgcgac ctgatcgccg cccgcgaaga tctcaatgcc gcgcgcttcg
     7261 agcgcgacaa gcgcaaccgg cgcggttacc caaccctaga cctggtcgcg agttacgagg
     7321 accgcgacac cgacagcgac ctgctgggcg acacgcagca ggaagacgta acggtcggca
     7381 tccagctcga aataccgctg tataccggcg gccgtatcag ttccgaacag gccgaggccg
     7441 aggcgcaact ggtgtcggcg cagaacaaca cgctgctgca gacccggctg gcgtcgcagc
     7501 gctcgcgcat cgcctacctc gacgtcgtct ccggcatcag ccaggtcagc gcgctcgagc
     7561 aggccctgga gtcgagcaac gtcgcactgg aagccaccca ggctggtttc gaagtcggca
     7621 cgcggacctc ggtcgacgtg ctcgtgtcgc tgcgtgaaac ctatcgcgcg cagcgcgatt
     7681 acgcgagttc gcgctacgag ttcctgctga acaagctgcg cctgaaacag gccgccggca
     7741 cccttgcaca agaggatttg agcgaaatca atcgccggct gattcgccct tgagcgatgg
     7801 gcgcggatag ctccgtcact ttcagggctc gcgattacct gtcacctcga tttttgccga
     7861 tgtgggtcac catcggcctg ttgcgactgg tcgcgctgtt gccctacccc gcggcgatgg
     7921 cggcgggccg gctcctgggt cgcctcatct acctgatccc gggatcgaga agaaaggtcg
     7981 tcgacaccaa cctgcggcat tgctttccac aaaaatcggc cgcggaacgc gagcggatca
     8041 agcgcgcctg ctatcagaat atcggcgttt ccatcatcga gatggcgctc tgctggtggt
     8101 ggcccgaccg gcgcctcgaa ccgctggtcg agctcgaggg cagggagtat ctcgatgccg
     8161 tgctcgactc cggccgcggc gccatactgc tgtcgggtca cttcaccagc ctcgaaatcg
     8221 gcggccgcct gctatccctg tacctgccct tccaggccat gtaccgtacc cagcgcaacg
     8281 ccctgttcga cagttacctg tacacccggc gcagcagcta tctggcggac gtggtatcgc
     8341 gcaaggatac ccgcaaactg attcgcggca tccgcgacaa gatcccgacc tggtacgcgc
     8401 ctgaccagga tttccgccgt gagcgcaacg tcttcgctcc gttcctcggc gtgcccgcgg
     8461 caaccatcac cgccggctcg agactcgcac agaccaccgg cgcggcgatg ctgccctact
     8521 atcccgaacg caagctcgac ggctccggct acgttttaca aattggtccg ccgctggaaa
     8581 attttcccag cggcgacgat gtcgaggatg cgaccgcgat caatcgctcg atcggcgatt
     8641 tcgtgcgcct gcaccccgag aattacatgt ggattcacca gcgcttcaag acccggccac
     8701 ctggtgaacc gcgcttctac gattgagcgc cgcgtcgtga tcccggcttc gccaatcagc
     8761 ggataccgcc tgctcggttt gctgctctac ccgttctggt tactgcatgc gatacggcac
     8821 ggcatgcgcc atggccagcg agactacttt cgaatgcgcc tgtgcggat
//
LOCUS       DATCZV010000066         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_375443, whole genome shotgun sequence.
ACCESSION   DATCZV010000066 DATCZV010000000
VERSION     DATCZV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375443"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VIW27_01525"
     CDS             <1..410
                     /locus_tag="VIW27_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY5738369.1"
                     /translation="RVAFVVDEYGDIQGLVTIEDILEEIVGEFTTDPSALYQDFFQDA
                     DGSYLIDGSTHIRDINRNLGVLLPSHGPRTLNGLILEHMEFIPEAGTSVKINGYPIEI
                     MQVKNNMVKTARLALPEDHQLAAAHGEPKPDDD"
     gene            465..1829
                     /gene="radA"
                     /locus_tag="VIW27_01530"
     CDS             465..1829
                     /gene="radA"
                     /locus_tag="VIW27_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY5738370.1"
                     /translation="MAAKSAARYQCRECQSLLPKWSGQCPECKSWNSIDELPATAATP
                     GARSSWHGRGRGPEPVALAEVPSGDNPRFTTAIDELDRVLGGGLVEGSVTLLGGDPGI
                     GKSTLLLQCLASISERREALYVTGEESPQQVSMRARRLGLPRDKLKLLSSTCVEEILA
                     RADAGKPAVMVIDSIQTIYSDSLQSAPGSVGQIRESAAMLVRFAKQSNVAIFLVGHVT
                     KEGQLAGPRVLEHMVDTVLYFEGDSDTRYRVVRAVKNRFGAVNELGIFAMTDGGLKTV
                     SNPSAIFLARHEQPVSGSVIMVSREGTRPFLIEIQALVSPSHGGNPRRLSIGLESNRL
                     AMLIAILQRHGNIPLHDQDVFINAVGGVRIAETGADLAIILAILSSYRDRPLDSRRLV
                     FGEVGLSGEIRPVSNGEERLREAAKHGFESAIIPTANRPRRGVLEDLELIPVNHLADT
                     LDAL"
     gene            1858..2103
                     /locus_tag="VIW27_01535"
     CDS             1858..2103
                     /locus_tag="VIW27_01535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY5738371.1"
                     /translation="MAENQDNLKDFEKTLQQLEKIVSRMESGELGLEDSLEQFETGVK
                     LAKSCQDRLASAELRVSQLIEKNGLQQTVPLEELDED"
BASE COUNT          443 a          669 c          642 g          361 t
ORIGIN      
        1 gccgcgtcgc cttcgtcgtc gacgagtacg gcgacattca agggctggtc accatcgagg
       61 acatcctcga ggaaatcgtc ggcgaattca ccaccgaccc gagcgcgctc taccaggact
      121 tttttcagga tgccgacggc agctacctga tcgacggctc cacgcacatt cgcgacatca
      181 atcgcaacct cggcgtgctg ctgcccagtc acggcccgcg cactctcaac ggcctgatcc
      241 tcgagcacat ggaattcatt cccgaagccg gcaccagcgt caagatcaac ggctacccga
      301 tcgaaatcat gcaagtcaag aacaacatgg tcaaaaccgc caggctggca ctgcccgagg
      361 accaccagct cgcggccgcg catggcgagc caaagcccga tgacgattag gcctgtcgac
      421 actgccggcc gcgcgacgat ctgtcgcccg ccgcgagtat tctgatggca gcgaaaagcg
      481 cggcgcgcta tcaatgccgg gaatgccaga gcctactgcc gaaatggtcc ggacaatgcc
      541 cggagtgcaa gtcctggaat agcatcgacg aattacccgc gacggcggca acacccggtg
      601 cgcgcagcag ctggcacggc cgtgggcgcg gtcccgagcc ggtcgcgctc gccgaggttc
      661 cgagcggtga caatccgcgt ttcacgaccg ccatcgacga actcgaccgc gtgcttggcg
      721 gcgggctggt ggaaggttcg gtaaccctgc tcggcggtga tccggggatc ggcaaatcga
      781 ccctgttgct gcaatgcctg gcatcgataa gcgagcgccg cgaggctctc tacgtgaccg
      841 gcgaagaatc gccgcagcag gtcagcatgc gcgctcgccg gctgggcctg ccgcgcgata
      901 agctgaagct gctaagttcg acctgtgtcg aggaaattct ggctcgcgcc gatgccggca
      961 aacccgcggt gatggtgatc gattcgatcc agacgatcta cagcgacagc ctgcaatcgg
     1021 cgccgggctc ggtgggccag ataagggaga gcgcggccat gctggtgcgc ttcgccaagc
     1081 agtcgaatgt cgctatcttc ctggtcggcc acgtgaccaa ggaaggccag ctcgccggac
     1141 cgcgcgtact cgagcacatg gtcgacaccg tgctctactt cgagggcgac agcgacaccc
     1201 gctaccgggt cgtccgtgcg gtcaagaacc gcttcggcgc ggtcaacgaa ctcggcatct
     1261 tcgcgatgac cgacggcggc ctgaaaacgg tcagcaaccc gtcggcgatc tttctcgctc
     1321 gtcacgagca gccggtatcg ggcagcgtca tcatggtttc gcgcgagggt acgcgaccct
     1381 tcctgatcga gatccaggca ctggtcagtc ccagccacgg cggcaatccg cgccgcctca
     1441 gcatcggcct cgaatccaac cggctggcga tgctgatcgc cattttgcag cgtcatggaa
     1501 atattccgct gcacgaccag gacgtgttca tcaatgccgt cggtggcgtg cgtatcgccg
     1561 agaccggcgc cgacctggcg atcatcctgg cgatcctgtc gagttatcgc gatcgcccgc
     1621 tcgattcgcg ccggctggta ttcggcgaag tcggactgtc gggcgagatc cgcccggtat
     1681 cgaacggcga ggagcgcctg cgcgaagccg ccaagcacgg cttcgaaagc gcgatcatac
     1741 ccacggctaa ccggccgcga cgcggtgtcc tcgaggatct cgaactaatc ccggtcaacc
     1801 atttggctga tactctggat gctttataat caccgctttg tgaatgcgga tcgcacgatg
     1861 gccgaaaacc aagataacct gaaagatttc gaaaaaaccc tgcaacagct agagaaaatc
     1921 gtcagtcgca tggaatccgg ggaactcggc ctcgaggatt cgctggaaca attcgaaacc
     1981 ggcgtcaagc tggcgaaaag ttgccaggac cggctggcca gcgccgagtt gcgcgtcagc
     2041 caactgatcg agaaaaacgg cttgcagcaa acggtgccgc tagaagaact agatgaagat
     2101 tgatgcacgg gtttt
//
LOCUS       DATCZV010000067         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_163530, whole genome shotgun sequence.
ACCESSION   DATCZV010000067 DATCZV010000000
VERSION     DATCZV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163530"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIW27_01540"
     CDS             complement(<1..309)
                     /locus_tag="VIW27_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5738372.1"
                     /translation="MLFFIEVVLSGLMAGVMYSLVALGFVLIFKASGIFNFAQGVLAL
                     FAALTLVGLMEKFHFPVWLAIIGTIAVMILLAWLIERLMLRHLVNQEPIILFMSTIGL
                     A"
     gene            complement(309..437)
                     /locus_tag="VIW27_01545"
     CDS             complement(309..437)
                     /locus_tag="VIW27_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738373.1"
                     /translation="MLFFTNPVIPEAAQRLSGIFVMCRDMNTGFLAAVAVRRHGSA"
     gene            complement(619..1422)
                     /locus_tag="VIW27_01550"
     CDS             complement(619..1422)
                     /locus_tag="VIW27_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5738374.1"
                     /translation="MSDKQADDEIILKVENISLSFGVMKVLDNISFDIRRGEIRSIIG
                     PNGAGKSSLLNVINGFYHPQQGTITFKGETRRQMAPHVAASHGIARTFQNIALFKGMS
                     TLDNIMTGRNLKMKTNIFWQALHLGPARAEELENRLEAEKIIDFLDIQAIRKKPVGKL
                     PYGLQKRVELGRALAAEPELLLLDEPMAGMNSEEKEDMCRFILDVNSEFGTTICLIEH
                     DMGVVMDLSDRVLVLDYGKQLADGLPDEVRNNQDVIDAYLGVPHEEDVA"
     gene            complement(1419..>3310)
                     /locus_tag="VIW27_01555"
     CDS             complement(1419..>3310)
                     /locus_tag="VIW27_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5738375.1"
                     /translation="AMREKDLGIWQSWTWAEAALEIHKLACGLASLGIKRGDKMAIIG
                     DNRPRLYWGMTAAQALGAVPVPLYQDSVADEMAFVLDNADVRIALVEDQEQVDKLLEV
                     LERCPRVEHIIYDDTRGLEKYRQDFLHDFESIQVAGEAFDREHPDYYDREVAAGNGSE
                     VCIMLYTSGTTGNPKGVVLTNDNAIITARNGIIREGLTADEEVLAYLPMAWVGDNLFS
                     YAQSYVAGFCVSCPEDPTTIPNDLREIGPTYFFAPPRIYENMLTQVMIRMEDAASIKQ
                     RMFNYFMELARQVGVKILNGEPVSLKNRLLYALGDLLVYSPLKDVLGLRRTRLAYTAG
                     EAIGPEIFDFYRSLGINIKQLYGQTECMVFICVQPDGEVYADTVGTPAIDVEIKIDDN
                     GEILYRSPGVFQAYYKNPDSTASTKTEDGWVHTGDAGFFDEHNGHLKIIDRAKDVGKL
                     NDGTLFAPKYIENKLKFFSFIKEAVVFGNGRDHSAAFINIDLDAVGNWAERNNIGYSG
                     YIDLSGRPEVYEQVKQCIDKVNRDLSQDSKLANSQIRRFLILHKELDADDGELTRTRK
                     VRRSHVGERYAELIDALYSNQPECHVETRVTFENGTIGSLAADVKIREAEYYPPQGAA
                     REAAA"
BASE COUNT          596 a          961 c         1068 g          685 t
ORIGIN      
        1 ggcgagaccg atggtggaca tgaacaggat gatcggttcc tggttgacca ggtggcgcag
       61 catcagccgc tcgatcagcc aggcgagcag gatcatcacc gcgatggtgc cgatgatcgc
      121 cagccacacc gggaaatgaa acttttccat caacccgacc agggtcagcg ccgcgaacag
      181 cgccagcacg ccctgcgcaa agttgaagat gccggaagcc ttgaagatca gcacgaaacc
      241 gagcgccacc agcgagtaca tgacgcccgc catcaggccg ctcaatacca cctcgataaa
      301 gaatagcatc aggcgctccc gtggcggcgc accgcgaccg cggccaaaaa gccggtattc
      361 atatcacggc acatcacaaa gatcccggat agccgctgcg cggcttccgg gatgacgggg
      421 ttagtgaaaa acagcatcag tcgatgtcct ccgaatagcc gtcaccccgg ccctcgagcc
      481 ggggtctatt gcaggaatcg aacaccgcgt ccaggcaagc ccgtcgggct tgctgctgta
      541 tcaacgaggg gggagtgtaa tggatgccgg cctgcgccgg catgacgatg gggtggggca
      601 gaatgcgatc aaacaacatc aggcgacatc ctcctcgtgc gggacgccga ggtaggcatc
      661 gatcacgtcc tggttattgc gtacttcgtc gggcaggccg tcggctagct gcttgccgta
      721 atccagcacc agcactcggt cggacaggtc catgaccacg cccatgtcgt gctcgatcag
      781 gcagatcgtg gtgccgaact ccgagttgac gtcgaggatg aagcggcaca tgtcctcttt
      841 ttcctcgctg ttcatgccgg ccatgggttc gtcgagcagc agcaactcgg gctcggcggc
      901 gagcgcgcgg ccgagctcga cgcgcttttg cagaccgtag ggcagcttgc cgaccggctt
      961 cttgcgaatc gcctggatgt cgaggaaatc gatgatcttc tcggcctcga gccggttctc
     1021 gagttcctcg gcgcgcgccg gaccgaggtg caaggcctgc cagaagatgt tggttttcat
     1081 cttcaggttg cgcccggtca tgatgttgtc gagcgtcgac atgcccttga acagcgcgat
     1141 gttctgaaag gtgcgcgcga tgccgtggct ggcggcgacg tgcggcgcca tctgccggcg
     1201 ggtctcgccc ttgaaggtga tcgtgccttg ctgcgggtgg tagaaaccgt tgatcacgtt
     1261 gagcagcgaa ctcttgccgg cgccgttggg gccgatgatg gaacggattt caccgcggcg
     1321 gatatcgaag ctgatattgt cgagcacctt catgacgccg aacgacaggc tgatgttttc
     1381 gaccttgaga atgatttcgt cgtcggcttg cttgtcgctc atgcggcggc ctcccgggca
     1441 gcgccttgcg gcgggtaata ctcggcttcg cggattttta cgtcggcggc gagcgagccg
     1501 atagtgccgt tttcgaaggt gacccgggtt tccacgtggc attccggctg gttcgagtag
     1561 agcgcatcga tcagctcggc gtagcgttcg ccgacatggc tgcgccgcac cttgcgcgtg
     1621 cgtgtcagtt cgccgtcgtc ggcgtcgagt tccttgtgca ggatcaggaa acggcgaatc
     1681 tgcgaattgg cgagcttgct gtcctgcgac aggtcccggt tgaccttgtc gatgcactgc
     1741 ttgacttgtt cgtagacctc ggggcggccg gacaggtcga tgtagcccga gtagccgata
     1801 ttgttgcgct cggcccagtt gccgaccgcg tcgagatcga tgttgatgaa agcggcgctg
     1861 tggtcgcggc cgttgccgaa aaccacggct tccttgataa aggaaaaaaa cttgagcttg
     1921 ttctcgatgt acttgggcgc gaacagcgta ccgtcgttga gtttgccgac gtccttggcg
     1981 cggtcgatga tcttcaggtg cccattgtgt tcgtcgaaga acccggcgtc gccggtgtgc
     2041 acccaaccgt cctcggtctt ggtgctggcg gtcgaatcgg gattcttgta gtaagcctgg
     2101 aaaacgccgg ggctgcggta gaggatttcg ccgttgtcgt cgatcttgat ttcgacgtcg
     2161 atcgccggcg tgccgacggt gtcggcatag acctcgccgt ccggctgcac gcaaatgaat
     2221 accatgcact cggtctgccc gtagagttgc ttgatgttga tgccgagcga gcggtagaaa
     2281 tcgaaaattt cggggccgat ggcctcgccg gcggtgtagg cgaggcgggt tcgccgcagg
     2341 cccagcacgt ccttcagcgg gctgtagacg agcaggtcgc cgagcgcata cagcaggcgg
     2401 ttttttagcg agaccggctc gccgttcagt atcttcacgc cgacctggcg cgcgagttcc
     2461 atgaaatagt tgaacattcg ctgcttgatc gacgccgcgt cctccatgcg aatcatcacc
     2521 tgggtcagca tgttttcata gattcgcggc ggcgcgaaga agtaggttgg gccgatttca
     2581 cgcagatcgt tgggaatcgt ggtcggatct tccgggcagc tgacgcaaaa gccggcgacg
     2641 taggattgcg cgtaggagaa caggttgtcg ccgacccagg ccatcggcag gtaggcgagc
     2701 acttcctcgt cggcggtcag gccctcgcgg atgataccgt tgcgcgcggt aatgatggcg
     2761 ttatcgttgg tcagcaccac gcccttggga ttgccggtgg tgcccgaggt atagagcatg
     2821 atgcagactt cgctgccatt gccggcggca acctctcgat cgtaatagtc gggatgttcg
     2881 cggtcgaagg cctcgcccgc cacctggatc gattcgaagt cgtgcaggaa atcctggcga
     2941 tatttttcca ggccgcgggt gtcgtcgtaa atgatgtgct cgacccgcgg gcagcgctcg
     3001 agcacttcga gcagcttgtc gacctgctcc tggtcctcga ccagcgcgat cctgacgtcg
     3061 gcgttgtcga ggacgaaggc catttcgtcg gccaccgaat cctggtagag cggcaccggt
     3121 accgcgccga gcgcctgcgc cgcggtcata ccccagtaaa gccgcggccg gttgtcgccg
     3181 atgatcgcca tcttgtcgcc gcgcttgatg ccgaggctgg cgagtccgca ggcgagcttg
     3241 tgaatttcga gcgccgcctc ggcccaggtc caggattgcc agatgccgag gtctttctcg
     3301 cgcatcgcgt
//
LOCUS       DATCZV010000068         5002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_587346, whole genome shotgun sequence.
ACCESSION   DATCZV010000068 DATCZV010000000
VERSION     DATCZV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587346"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VIW27_01560"
     CDS             complement(<1..810)
                     /locus_tag="VIW27_01560"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY5738376.1"
                     /translation="MRAFAWFGALILLSVSCGARAESFVVENIEVDGIKKITVGTVFS
                     YLPINVGESLDLERTPEIIRELYSTGFFDDIELLRRDNTLVIRVIERPSIAEVNFEGN
                     DDIEDDALKEALEQTGMSKGRIFDENKLDKLELELQQVYYSLGKYAARIEASWRKLDE
                     DRIAIDIDISEGVSATIKSINITGNQNFDQDDLLEVFQLEPTDSGWFADDKYSSSKLV
                     ADLESLKSFYLDRGYIQFEVESQQVTISPDRRDISITVNVREGEQYRVGKIS"
     gene            complement(813..2183)
                     /gene="rseP"
                     /locus_tag="VIW27_01565"
     CDS             complement(813..2183)
                     /gene="rseP"
                     /locus_tag="VIW27_01565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HEY5738377.1"
                     /translation="MGILQSLLAFIVAIGVLVTVHEFGHFWVARRMGVKVLRFSVGFG
                     RPLWRWRSNKDQTEYVIGSLPLGGYVKMLDEREGHVDRRELDYSFNRKSVWQRSAIVA
                     AGPLFNFLFAILAYYLIFLAGIGGMKPVLGEVANPSLAHDAGLRQGDVILAVNGIETR
                     TWEKARLVLLDQAVDADRLRLQVQDEDLQISDRTVDISGLDFLQAEQIDLLGDLGIAP
                     WQPNLPPIIDEVLADGAGERAGLQSGDRVVSLDGVEIDNSNRWIGMIRENPQTTMRLR
                     IVRDGESMELELTPDLRDEDGARYGYIGVRSRVEIPESLRREMTVVERYGPLEGFAQA
                     LDKTWRMTWLTLRVLGKLVTGEASVRNLSGPITIAHYAGISASIGLEPFLGFLAIISI
                     SLGVLNLLPVPMLDGGHLFYYLIEIFKGSPVSEQTEIIGQKIGIALLFCLMSIALYND
                     LLRLVE"
     gene            complement(2189..3370)
                     /gene="ispC"
                     /locus_tag="VIW27_01570"
     CDS             complement(2189..3370)
                     /gene="ispC"
                     /locus_tag="VIW27_01570"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5738378.1"
                     /translation="MRTLTLLGATGSIGQSTLDIVARHRDRFAVHALTANNNFEKMLE
                     LCRQFEPRYAVMSNADSARRLEDAIDDLDTRVLAGAEGLEEVAAAPEVDTVVAAIVGA
                     AGLLPTLAAVRAGKRVLLANKESLVMAGALFMDEVRRHGAELLPVDSEHNAVFQCLPP
                     GGVGHDARLQGVRRIILTGSGGPFRDRPLDDLQQVTPAEACAHPNWDMGPKISVDSAT
                     MMNKGLEMIEACWLFDVDHSVIEILLHRQSIVHSMVSYLDGSVIAQLGNPDMRTPIAN
                     ALAWPERIDSGVEALDLRRISQLDFSPADENRYPSLALAREAWHRGGTGMAVLNAANE
                     VAVEHFLDGRIGFNESARVIAGVMENSPTSEADSLEKILQADASARELASEIAGGNRL
                     K"
     gene            complement(3367..4182)
                     /locus_tag="VIW27_01575"
     CDS             complement(3367..4182)
                     /locus_tag="VIW27_01575"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY5738379.1"
                     /translation="MLKQRILTALILAVVFVGCILFTETRWVGLLFSIVLFAATRELL
                     ALTLRLSLPVTLLLSAVFALLFWWSLSIANPVLVQWQALAGLVLWLLIAVGLVFYRHH
                     GNWPLLARVLMLGLGLDLLWICVHCLVYLHYVYGGWTLLFMFTLVWVVDIGAYFCGRR
                     FGKHKLAPVISPGKTWEGVFGGLAATLAWMLLVYALVDGLQPGLAAYLAIGLATVSVS
                     IVGDLFESVLKREAGVKDSGALLPGHGGVLDRIDSVIAAAPVFVTGLMLAGSA"
     gene            complement(4176..4925)
                     /gene="uppS"
                     /locus_tag="VIW27_01580"
     CDS             complement(4176..4925)
                     /gene="uppS"
                     /locus_tag="VIW27_01580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020751059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY5738380.1"
                     /translation="MTASQTEASIPRHVCIIMDGNGRWAKKRLMPRSFGHRKGVETTR
                     RAVEYFARSGVENLTLFAFSSENWQRPADEVDSLMGLFMQALQEYTGELNQQQIRLRF
                     IGDRNPFSAELRARIEQTEAETAANRRMVLNIAANYGGRWDIASAARRLAERSRDGSL
                     DPQSIDETVFGDELSLGDGPPPDLFIRTGGEQRISNFLLWQLAYTELYFSDVLWPDFS
                     EAEMRAALAEYSRRQRRYGKTSEQLESVTSC"
BASE COUNT          981 a         1606 c         1515 g          900 t
ORIGIN      
        1 gctgatcttg ccgacgcggt attgctcgcc ctcgcgtacg ttgacggtaa tcgatatgtc
       61 cctgcgatcg gggctgatgg ttacctgctg tgactcgacc tcgaactgaa tgtaaccgcg
      121 gtcgaggtag aaggatttca gcgactcgag gtcggcgacg agcttggaac tcgagtactt
      181 gtcatcggcg aaccagcccg aatcggtggg ctcgagctga aacacctcga gcaggtcgtc
      241 ctggtcgaaa ttctggttgc cggtgatatt gatcgacttg atagtcgccg aaacgccttc
      301 cgaaatatcg atgtcgatgg cgatcctgtc ctcgtcgagc ttgcgccagc tggcctcgat
      361 acgcgccgcg tacttgccga gcgagtaata gacctgctgc aattcgagtt cgagcttgtc
      421 gagcttgttt tcgtcgaaaa tgcggccctt tgacatgccg gtctgctcga gcgcctcctt
      481 caacgcatcg tcttcgatat cgtcgttacc ctcgaaattg acctcggcga tcgacggtcg
      541 ctcgatcacg cggatcacca gcgtgttatc gcgccgcaac agttcgatat cgtcgaagaa
      601 gccggtcgaa tagagttcgc ggatgatttc cggcgtacgc tcgaggtcga ggctctcgcc
      661 gacgttgatc ggcaggtagc tgaataccgt gccgacggtg attttcttga tcccgtcgac
      721 ctcgatattc tcgaccacga agctttccgc gcgggccccg caggaaaccg acaacaggat
      781 tagtgcaccg aaccaggcga acgctctcaa acttactcta ccaggcgcaa caagtcgtta
      841 tatagcgcga tcgacatcag gcaaaacagc agcgcgatgc cgattttctg gcctattatc
      901 tcggtctgct cggagaccgg gctgcccttg aaaatctcga tcaggtagta aaacaggtga
      961 ccgccgtcga gcatcggtac cggcaacaga ttgagcacgc cgaggctaat gctgatgatt
     1021 gccaggaaac cgaggaatgg ctcgagaccg atgctcgccg agataccggc gtaatgagcg
     1081 atggtgatcg ggccgctcag gttcctgacg ctggcttctc cggtgaccag cttgccgagc
     1141 acccgcaatg tcagccaggt catgcgccag gtcttgtcga gcgcctgcgc gaagccctcg
     1201 agcggaccgt agcgctcgac cacggtcatc tcgcggcgca gcgactcggg tatttccacg
     1261 cggctgcgaa cgccgatgta accataccgg gcgccatcct cgtcgcgaag atccggcgtc
     1321 agctcgagtt ccatcgactc accgtcgcgg acgatgcgca gccgcatggt agtctgcggg
     1381 ttttcgcgaa tcatgccgat ccaacggtta gaattgtcga tctcgacacc gtccagcgag
     1441 accacgcggt cgccactctg cagcccggcg cgttcgccgg cgccatccgc cagtacctcg
     1501 tcgatgatcg gcggcaagtt cggctgccag ggcgcgatgc cgaggtcgcc caacaggtcg
     1561 atctgctccg cctgcaaaaa atcgaggcca ctgatatcga ccgtccggtc gctgatctgc
     1621 aaatcctcgt cctgcacctg cagacgcagc cggtcggcat cgaccgcctg atcgagcagc
     1681 accaaacggg ccttttccca ggtgcgggtc tcgatgccgt tgaccgccag aatgacatcc
     1741 ccctgccgca agccggcgtc gtgggccagg ctggggttgg caacctcacc gagcaccggt
     1801 ttcataccgc cgatcccggc gaggaatatc aggtagtagg cgagaatcgc gaacaggaag
     1861 ttgaacagcg gaccagcggc aacgatggcg gagcgttgcc agacggattt gcggttgaaa
     1921 ctgtagtcga gttcgcgccg gtcgacatgg ccctcgcgct cgtcgagcat tttcacgtag
     1981 ccgccgagcg gcaggctgcc gatcacgtac tcggtctgat ccttgttcga gcgccagcgc
     2041 cacaacggcc tgccgaagcc gaccgagaag cgcaatacct tgacccccat gcgtcgcgca
     2101 acccagaagt gaccgaactc gtgcaccgtc accagcacgc cgatggcgac gatgaaggcg
     2161 agcaagcttt gtaatatacc cagtaaattc atttcaatcg gttcccgccg gcaatttcgg
     2221 acgccagttc gcgagcgctg gcgtcggcct gcaaaatctt ctccaggctg tcggcttcgc
     2281 tggtgggcga attttccatc acgccggcga tcaccctggc actctcgttg aagccgatac
     2341 ggccatccag aaaatgttcg accgcgacct cgttggccgc gttcaatacc gccatgccgg
     2401 tgccgccacg atgccaggct tcgcgcgcca gcgccaggct cggatagcgg ttttcgtcgg
     2461 ccggcgaaaa atccagctgg ctgatgcggc gcaggtcgag ggcttcgaca ccgctgtcga
     2521 ttcgttccgg ccaggccagc gcgttggcga tcggcgtgcg catgtccggg ttgccgagtt
     2581 gcgcgatcac cgagccgtcg aggtaggata ccatcgagtg cacgatactc tggcggtgca
     2641 gcagaatttc gatcaccgag tgatcgacat cgaacagcca gcaggcttcg atcatctcca
     2701 gacccttgtt catcatggtc gccgaatcga cggaaatttt cggccccatg tcccagttcg
     2761 ggtgcgcaca ggcttccgcc ggcgtcacct gctgcaggtc gtcgagcggg cggtcgcgaa
     2821 acggcccgcc ggagccggtc aaaatgatgc gccgtacgcc ctgcaggcgg gcatcgtggc
     2881 cgacgccgcc cggcggcagg cactgaaaca cggcattgtg ttcgctgtcg acaggcagca
     2941 gttcggcgcc gtgccgacgc acctcgtcca tgaacagggc ccccgccatc accagcgact
     3001 ccttgttggc gagcagcacg cgcttgccgg cacgcaccgc ggccagcgtc ggcagcagtc
     3061 cggcggcgcc gacgatggcg gcgacgacgg tatcgacctc gggtgcggcg gccacttcct
     3121 cgagcccctc ggcaccggcg agcacccggg tatcgagatc gtcgatcgcg tcttcgagcc
     3181 gccgcgcgct gtcggcattc gacatcaccg cgtagcgcgg ctcgaactgc cggcacaatt
     3241 cgagcatttt ctcgaaattg ttgttggcgg tcagcgcatg caccgcgaag cggtcgcggt
     3301 ggcgagcgac gatatcgagc gtggactggc cgatcgagcc ggtcgcaccg agcagcgtca
     3361 acgtcctcat gccgaacccg ccagcatcaa accggtaaca aacaccggtg ccgcggcgat
     3421 cacgctgtcg atgcggtcga gcacgccgcc gtgaccgggc agcaacgcac cgctgtcctt
     3481 gacgccggcc tcgcgcttga gcacgctctc gaacaggtcg ccgacgatgg ataccgatac
     3541 cgtggccagg ccgatcgcca ggtaggccgc gagacccggt tgcaggccgt cgaccagcgc
     3601 gtagaccaac agcatccagg caagggtcgc cgccagcccg ccgaataccc cttcccaggt
     3661 cttgccgggg ctgatcaccg gggccagctt gtgcttgccg aagcgccgtc cgcagaaata
     3721 ggcgccgatg tcgaccaccc acaccagcgt gaacatgaac aacagggtcc agccgccgta
     3781 gacgtagtgc agatagacca ggcaatgcac gcagatccac aacaggtcga gcccgagacc
     3841 gagcatcaga acccgcgcca gcagtggcca gttgccgtga tggcgataaa aaaccaggcc
     3901 caccgcgatc agcagccaca ataccagccc ggccagcgcc tgccactgga ccagcaccgg
     3961 gttggcgatc gacagcgacc accagaacag cagcgcgaac accgccgaca acagcagcgt
     4021 caccggcagc gacagacgca gggtcagggc cagcagctcg cgagtcgccg cgaacagcac
     4081 gatgctaaac agcaggccga cccagcgggt ttcggtaaac aggatgcagc cgacgaacac
     4141 gacggcgagg atcagcgcgg tcaggattcg ttgtttcagc acgatgtgac ggactccagc
     4201 tgctcgctgg tcttgccgta gcgtcgctgg cggcgggaat actcggcgag cgcggcgcgc
     4261 atctcggctt ccgagaaatc cggccacagc acgtcgctga aatagagctc ggtgtaggcg
     4321 agctgccaga gcaggaaatt actgatgcgc tgctcaccgc cggtgcggat gaaaaggtcc
     4381 ggcggcggcc cgtcgccgag cgacaactcg tcgccgaaaa cggtctcgtc gatactttgc
     4441 ggatcgaggc tgccgtcgcg gctgcgctcg gcgagacgcc gcgcggcgct ggcgatatcc
     4501 cagcgaccgc cgtaattggc ggcgatattc aacaccatgc gccggttggc ggcggtttcc
     4561 gcttcggttt gctcgatccg cgcgcgcaat tcggcggaga acgggttgcg gtcgccgatg
     4621 aagcgcagcc ggatctgctg ctggttcaat tcgcccgtgt attcctgtaa tgcctgcatg
     4681 aacaatccca tcaggctgtc gacctcgtcg gcgggacgct gccagttctc gctgctgaag
     4741 gcgaacagcg tcaggttctc gacaccgctg cgagcgaaat actctaccgc ccgtcgcgtg
     4801 gtctcgacac ccttgcgatg accgaagctg cgcggcatca gccgcttctt cgcccagcgg
     4861 ccgttaccgt ccatgatgat acagacatgg cgcggtatcg aggcctcggt ctgactggcc
     4921 gtgatcgctt gagaagacat aatatcgtta cgctaccgcg atcagatctc cattaactcc
     4981 ttttccttga cctgcaacaa gg
//
LOCUS       DATCZV010000069         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_269770, whole genome shotgun sequence.
ACCESSION   DATCZV010000069 DATCZV010000000
VERSION     DATCZV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269770"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VIW27_01585"
     CDS             complement(<1..175)
                     /locus_tag="VIW27_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5738381.1"
                     /translation="MKKSLILASIALLPLGTLQAADYTIDPGHTYASFAINHLGFSTM
                     RGKFNEQKGTMKYD"
     gene            complement(233..1081)
                     /locus_tag="VIW27_01590"
     CDS             complement(233..1081)
                     /locus_tag="VIW27_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738382.1"
                     /translation="MEQLLDRPEIQSAVIPFIVGLAVAAGLRKITRGAWIWALFAAFL
                     CSALLINGTTLMPLTGTRKIILLVLGACLVGGLGAFVLRTTNLQRQAATGLVIISVLW
                     IFWTVMERREATAAAIFVAGAVALNLFTAWGLDLCRKHEARLHGAGFGLLLGTGLCAA
                     LGSSALLGQLGLALAAGCGGLFLAWVLLPGQTGGELIHGRPLAILPYLMPAVTIGLAA
                     VIFARLPWYALAPLAAIPLATSLVPYQSESRFMNALVSSLPGLIITGATAFWVWQAGS
                     SSASGY"
     gene            complement(1289..2176)
                     /locus_tag="VIW27_01595"
     CDS             complement(1289..2176)
                     /locus_tag="VIW27_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5738383.1"
                     /translation="MPGPTLAARLLALLLVALCPLPAGAQDGQVSPLIERGAYLMQLS
                     GCRHCHTAEDGEPFAGGRPLETPFGTFYTPNITADDSTGIGAWSETDFVRALRHGETP
                     DGSHYYPAFPYTSYTRMTDEDARALFAYLRSQTASKRTNREHDLAWYLQWRLAAQAWK
                     WLFFTPGEYQPDNARSPAWNRGAYIAEALAHCGECHTPRNWFGAANRELAYAGNPDGP
                     DDELVPNITPDPETGTGDWSRDSLDLFLRFGELPNGEYTAGSMDYVITGIGKLTETDR
                     AALIDYLRALPPIRNSVSN"
     gene            complement(2294..>2496)
                     /locus_tag="VIW27_01600"
     CDS             complement(2294..>2496)
                     /locus_tag="VIW27_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5738384.1"
                     /translation="AYPGKTTALPKIWDTYPEISEYGKKYKQAVTQLAAVAGNGVDAL
                     KPAVGELGKSCKGCHDEFREKK"
BASE COUNT          435 a          751 c          845 g          465 t
ORIGIN      
        1 ggtcgtattt catcgttccc ttctgttcgt tgaacttgcc acgcatcgtc gaaaagccga
       61 gatggttgat ggcgaagctg gcataggtat gcccgggatc gatggtgtaa tcggcggcct
      121 gcagcgtgcc cagcggtaac agtgcgatgc tggcgaggat caagcttttc ttcatgaatg
      181 tctccttggg ttttacatgg ttaaaagagt cgcccggtcg ggcggttgac gatcaatatc
      241 cggacgcgct cgaactgcca gcctgccaga cccagaaggc cgtggcgccg gtaattatca
      301 agccgggcag gctgctgacc agcgcgttca tgaaacggga ttcggactgg tagggcacca
      361 ggctggtagc cagcggtatc gctgccagcg gcgccagcgc gtaccagggc aggcgggcga
      421 aaatgaccgc ggccaggccg atggttaccg ccggcatcag gtagggaagt atcgccagcg
      481 gccggccatg gatcagctca ccgccggtct ggccgggcag cagtacccag gccaggaaca
      541 ggccgccgca gccggcggcc agcgccagac ccagttgccc gagcagggcc gaggagccca
      601 gcgccgcgca cagaccggtg ccgagcaaca gtccgaagcc ggcgccgtgc aggcgagctt
      661 cgtgcttgcg gcacagatcg agaccccagg cggtgaacag gttcagcgcc actgcgccgg
      721 cgacgaagat agcggcggcg gtcgcttcgc ggcgttccat aaccgtccag aagatccaga
      781 gcaccgagat gatgaccagg ccggtcgctg cctgtcgctg caggttggtc gtgcgtagca
      841 cgaacgcgcc gagacccccg accagacagg cgccgagtac cagcaggatg atcttgcggg
      901 tgccggtcaa cggcatcagc gtcgtgccgt tgatcagcag ggcgctgcac aggaaggcgg
      961 caaacagtgc ccagatccag gcgcccctgg tgatcttcct gagaccggcg gctaccgcca
     1021 ggccgacgat gaacggaatc accgcgctct ggatttcggg tctgtcgagg agctgttcca
     1081 tcagttgcgc agttctggca tgggtgtttt agtcatcggt caatagggcc ggtgcaggcg
     1141 tggcggatta tcgtatcagg ttatttgtcg acatcaagct ggttattttc gcgctggcct
     1201 gctgcattag tacgcagcat atatcgatgc ggattgcttt gcccggtggc cggcgattgc
     1261 ccggttagcg ataccgtcgt cgagcgggtc agttactaac ggaattgcga atcggcggta
     1321 acgcacgcag gtaatcgatc agcgccgcgc gatccgtctc ggtgagcttg ccgattccgg
     1381 tgatcacgta atccatcgaa cccgccgtgt attcaccgtt cggcagttcg ccgaaacgca
     1441 ggaacaagtc cagcgagtcc cggctccagt cgccagtacc ggtttccggg tcgggcgtga
     1501 tattgggcac cagttcgtca tcggggccgt cagggttgcc ggcataggcc agctcgcgat
     1561 tcgccgcgcc gaaccaattg cgcggcgtgt ggcattcacc gcaatgggcc agtgcctcgg
     1621 cgatgtaggc gccgcggttc cacgccgggc tgcgggcgtt atcgggttgg tattcaccgg
     1681 gagtgaaaaa cagccatttc caggcctgtg ccgccagtcg ccattgcagg taccaggcca
     1741 ggtcgtgctc gcggttggtg cgtttcgacg ccgtctgcga acgcaggtag gcgaacagcg
     1801 cgcgcgcgtc ctcgtcggtc atgcgcgtgt acgaggtgta cgggaacgcc gggtaatagt
     1861 ggctgccgtc gggcgtctcg ccatgtcgca gtgcgcgcac gaagtcggtt tcgctccatg
     1921 cgccgatacc ggtgctatca tcggcggtga tattgggggt gtaaaaggtg ccgaacgggg
     1981 tttccagcgg gcgaccgccg gcaaagggtt cgccgtcttc ggcggtgtga caatgacggc
     2041 agccgctgag ctgcatcagg tacgcgccgc gctcgatcag cggcgacacc tgcccgtcct
     2101 gggcgccggc gggcaacggg cacagcgcca ccagcagcaa ggccagcaag cgcgccgcaa
     2161 gtgtcgggcc gggcaacccg caacgcgttg ctgccgcgcc gcgggcattg cctgtccgcc
     2221 gccgctgcgg aaatcccgaa tggtggcggg cgcggaccct gttcgtgaca agcctcaggg
     2281 tgaccgcggc agcctatttc ttctcgcgga actcgtcgtg gcaacccttg caggatttgc
     2341 cgagttcgcc gaccgcgggt ttcagtgcgt cgacgccgtt gccggccacg gctgcgagtt
     2401 gggtcaccgc ctgcttgtac tttttaccgt actcgctgat ttcggggtaa gtatcccaga
     2461 tcttcggcag cgcggtggtc ttgccggggt aggctc
//
LOCUS       DATCZV010000070         4919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_5155, whole genome shotgun sequence.
ACCESSION   DATCZV010000070 DATCZV010000000
VERSION     DATCZV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5155"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VIW27_01605"
     CDS             <1..1428
                     /locus_tag="VIW27_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5738385.1"
                     /translation="RLGLPADRPFAELSGGFKRRALLGRALVGEPDLLLLDEPTNHLD
                     IESIVWLEELLLAFRGTLVFITHDRSFLQRLATRIVELDRGRLSSWPGDYRRYLEARA
                     AELETEARHNALFDKKLAQEEAWIRQGIKARRTRNEGRVRALERMRTERAQRRERSAA
                     PRLEAQQAEASGKIVIEAHELEFGWDGNPIVSNFSCKVLRGEKIGILGPNGCGKSTLI
                     KLLLGQLAPQSGWVRTGTRLEIAYFDQQREILDPELSVRDNLAVDGDQVTVNGRSRHP
                     VGYLKDFLFSEKSIHMPVKALSGGERNRLLLARLFTRSFNLLVMDEPTNDLDIETLEL
                     LEEMLVDYSGTLLLVSHDREFIDNCVTSTLVFEGRGRVNEYVGGYRDWLRHKPATPDT
                     PPPSRDKPRRSPSNSAGTQKELRALPGKIEKLEAKIEALQQKLAAPDYYQQEADVIRA
                     DQQQLKTLEAELQALYRRWEELESS"
     gene            complement(1437..1910)
                     /locus_tag="VIW27_01610"
     CDS             complement(1437..1910)
                     /locus_tag="VIW27_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5738386.1"
                     /translation="MKDANLRVKKVLEASGIDFEIRLLPAAVRTARLAADALGCAVGQ
                     IANSLIFRNLDDDNALLIMCAGDRRVDTDKVRAVTGLELGRADADFVRRETGFAIGGV
                     PPVGHSNPPVTILDRSLQRFDEIWAAAGTPESIFCMTPAQLQLITGGEWLDIAED"
     gene            2109..4340
                     /locus_tag="VIW27_01615"
     CDS             2109..4340
                     /locus_tag="VIW27_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5738387.1"
                     /translation="MVTGRLRLVSVVFAFLAYLSHPAHSASDDLGGSTRPRIGLVLSG
                     GGALGLAHIGVLQVLEEMRVPVDCVVGTSMGALVAGSYAAGVDPHSMQQAITATDINT
                     LFDDDPGRSEIPQRIKRDDYQPLFGFALGYNDGIQLPAGASSGYKFELFLKELIGSGA
                     SVAELDFDQLPIPYRAVATDLENGKMKVFDRGELPRVMRASMSLPSILAPTKIDNRSY
                     VDGGLVRNLPVDVGRELCGEVLIAVNLGTRPKTMDQVKNEFDVALQSLVLLTDQNVVR
                     SLDELQPRDVLIEPELDAFTSSSFSSQQEIIERGAAAARANRQQLSRLSLTQDEYHRW
                     QNQRRSKIPEAPRVTAITAEVKGAVSAKAVQRNLASGPGNEFEPARLHEEIIDIYGRG
                     DFSYTGYSIVPSADEATIVIKAESKPWGPGYLKLGLGAATDFSSPTQLNLATSYRRTW
                     INSLGGEWRSDAQIGYDSFLRFELFQPLQIRDGLFLSPYALTRRDFIQVYQEDIRLGD
                     LKIEQQQAGLDLGVSGPVGEIRLGGFVRNVTTKPGLGVIDPLFPNREASQTGVETRAV
                     IDQLDSYVFPRSGYYAFVDLRAVEQDDDAFEEYTRAQLGLAGAISSGDNTLSGKIEWG
                     DELSGVDDLPVFDVFRLGGPNRLAGLYLDQLNGTRYDLATLNYYHRYSNLPPQIGRGL
                     YVGVSLESGRVDDPLMKDPWERVNSAAIYWGADTLLGAVYIGYGRSSLNQSSFYLVIG
                     PKF"
     gene            4708..>4919
                     /locus_tag="VIW27_01620"
     CDS             4708..>4919
                     /locus_tag="VIW27_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY5738388.1"
                     /translation="MKHREASSDHLDVIPRSQHNISRKSISDSALKVLYRLNKSGHRA
                     CLVGGGVRDLLLGIQPKDFDVATDATP"
BASE COUNT         1013 a         1512 c         1496 g          898 t
ORIGIN      
        1 agactggggc tgcccgccga ccggccgttc gccgagcttt ccggtggctt caagcgccgc
       61 gcgctgctcg gccgggcact ggtcggcgag cccgacctgt tgctgctcga tgagcccacc
      121 aaccacctcg atatcgagtc gatcgtctgg ctcgaggaat tgctgctcgc gtttcgcggc
      181 acgctggtct tcatcactca cgaccgcagt tttctgcagc gcctggcgac ccgcatcgtc
      241 gagctcgacc gcggccgcct ctccagctgg cccggcgatt accggcgcta tctcgaagcg
      301 cgcgccgccg agctggaaac cgaggcccgc cacaatgcgc tgttcgacaa gaagctggcg
      361 caggaggaag cctggattcg gcaggggatc aaggcgcgcc gcacgcgcaa cgagggtcgc
      421 gtgcgggcgc tggaacggat gcgcaccgag cgcgcgcaac gtcgcgaacg cagcgccgcg
      481 ccgcggctcg aggcgcagca ggccgaagcc tcgggcaaga tcgtcatcga ggcgcacgaa
      541 ctcgagttcg gctgggacgg caaccccatc gtcagcaatt tcagctgcaa ggtcctgcgc
      601 ggcgaaaaaa tcggcatcct cggacccaac ggctgcggca aatcgacgct gatcaagttg
      661 ctgctcgggc agctggcgcc gcaatcgggc tgggttcgca ccggcacccg gctcgagatc
      721 gcctacttcg accagcagcg cgaaatcctc gaccccgaac tctcggtacg cgacaacctc
      781 gcggtcgacg gcgaccaggt gacggtcaat ggccgcagcc ggcacccggt gggctatctc
      841 aaggacttcc tgttcagcga aaagtcgatc cacatgccgg tcaaggcgtt gtcgggcggc
      901 gagcgcaacc gcctgctgct ggcgcgcctg ttcacgcgca gcttcaacct gctggtgatg
      961 gacgaaccga ccaacgacct agatatcgaa accctcgaac tgctcgagga gatgctggtc
     1021 gactacagtg gtacgctgct gctggtcagc cacgatcgtg aattcatcga taattgcgtg
     1081 accagcacgc tggtgttcga gggccggggc cgggtcaacg aatatgtcgg cggctatcgg
     1141 gactggctgc ggcataagcc ggcaaccccg gatacacctc cgccgtcgcg tgacaaaccg
     1201 cgtcgctcgc cgtcaaatag cgccggcacg caaaaagaat tacgcgccct gcccggtaag
     1261 atcgaaaaac tggaagcgaa aatcgaagcc ctgcaacaaa aactcgccgc gcccgactat
     1321 taccagcagg aagccgatgt gattcgcgcc gaccagcagc aactgaaaac cctggaagcg
     1381 gaactgcagg cactctatcg acgctgggaa gaactggaga gcagttgacc ctgcgctcaa
     1441 tcctcggcga tatccagcca ctcgccgccg gtaatcagct gcaattgcgc gggcgtcatg
     1501 caaaaaatcg attccggcgt gcccgcggcg gcccagattt cgtcgaagcg ttgcagcgag
     1561 cgatcgagga tcgtgactgg cggattactg tgcccaaccg gcggcacgcc gccgatcgcg
     1621 aaaccggttt cgcggcgcac gaagtcggcg tcggcgcggc ccagttcgag accggtaaca
     1681 gcgcgtacct tgtcggtgtc gacccggcgg tcgccggcgc acattatcag caacgcgttg
     1741 tcatcgtcga gattcctgaa gatcagtgaa ttggcgatct gaccgaccgc acagccgagt
     1801 gcatcggcgg cgaggcgcgc ggtgcgcacc gctgccggca acagcctgat ctcgaagtcg
     1861 atgcccgagg cttcgaggac tttcttcacc cgcaggttgg cgtctttcat ggtttaccca
     1921 aatacgggaa agcctagcag atcggtcggc aaaaaaggat gggcaacaaa cagggctgca
     1981 ggggattcgg cggctcgaag tacgcaccga caggagaaac cggcttttcc gcagagacct
     2041 gattgattgg tcgtgtttgg ttcctccgag ttaagatata ccgactggta tctacgctgg
     2101 atgggatcat ggttacaggc aggctgcgtt tggtttcggt ggtatttgct tttctggcgt
     2161 acctttcaca tccggcgcat tccgcatctg acgacctcgg gggatcgacg cgtcccagga
     2221 taggcctcgt tctctccggc ggcggcgccc tgggacttgc ccatatcggc gtgctacagg
     2281 tactggagga gatgcgtgtt ccggtcgact gcgtggtcgg aacgagcatg ggggcgctgg
     2341 tggccggcag ttatgccgcc ggcgtcgatc cgcacagcat gcaacaggcg attaccgcaa
     2401 ccgatatcaa caccctgttc gacgatgatc ccggacgctc ggagataccc caaaggatca
     2461 agcgcgacga ttaccagccg ctattcggtt tcgcgctcgg ttacaacgat ggaattcagc
     2521 tgccggccgg cgcatcctcc ggttataaat tcgagctctt cctcaaagaa ctgatcggta
     2581 gcggcgcctc ggttgccgag ctggatttcg atcaactgcc gattccctac cgggcggttg
     2641 caacggacct cgaaaacggc aagatgaaag tattcgatcg cggcgagttg cccagggtca
     2701 tgcgggccag catgtcgctg ccgtcgatac tggcgcccac gaaaatcgat aatcgcagct
     2761 atgtcgacgg cggtctggta agaaacctgc cggtagatgt cggccgggaa ctatgtggag
     2821 aagtgctcat agcagtcaat ctcggcaccc ggccaaagac catggaccag gtaaaaaacg
     2881 aattcgatgt cgccttgcag tcgctggtcc tgctgaccga ccagaacgtt gtcagatcgc
     2941 tcgacgaatt gcagccgcgg gacgtgctga tcgagcccga actcgacgcc ttcacatcgt
     3001 cgagtttttc cagtcagcag gaaataatcg agcgcggcgc tgccgcggcc agggctaacc
     3061 gacaacagtt atcgaggttg tccctgacgc aggatgaata ccaccgttgg cagaaccagc
     3121 ggcgcagcaa aatacccgaa gccccgcggg tgacggcgat caccgccgaa gtaaagggag
     3181 cggttagcgc aaaagccgtg caacgaaatc ttgccagcgg gcccggcaac gaattcgaac
     3241 ccgcaagact gcacgaggaa atcatcgaca tctacgggcg cggcgacttt tcctataccg
     3301 gctattcgat cgtgccatcc gctgacgaag cgaccatcgt catcaaggca gaaagcaaac
     3361 cctggggacc gggctatctg aagctgggcc tcggtgccgc gaccgatttc agcagcccga
     3421 cacagctcaa cctggcaacc agttaccggc gcacctggat aaattccctc ggtggcgagt
     3481 ggcgcagcga tgcgcagatc ggctacgact cgttcctgag gttcgagctg tttcagccgc
     3541 tgcagattcg ggacggcctt ttcctgagtc cgtacgcgct gaccaggaga gacttcatcc
     3601 aggtgtatca ggaagatatt cgactgggtg acctgaaaat agaacagcaa caggctggcc
     3661 tggacctggg tgtgagcggc cctgtcggcg aaatccgcct gggcggcttt gtcaggaacg
     3721 tcaccaccaa gcccggtctc ggcgtgatcg acccgctgtt ccccaatcga gaggcctcgc
     3781 aaacgggggt cgaaaccagg gctgttatcg atcagctgga cagctacgtg tttcccaggt
     3841 cggggtatta tgccttcgtg gatctcaggg ccgtggagca ggacgacgat gctttcgagg
     3901 aatacacccg cgcgcaactg ggccttgccg gcgccatcag ctctggcgat aacaccctga
     3961 gcgggaaaat cgaatggggc gacgagttga gcggagtcga cgacttgccg gtgttcgacg
     4021 tcttccggct tggcggaccc aaccggcttg ccggcctgta tctcgaccag ttgaacggga
     4081 ctcgctacga cctcgcaacg ctgaattatt atcaccgcta ctcgaatctg ccgccacaga
     4141 tcgggcgtgg cctgtacgtc ggcgtgtcgc tcgagtcggg ccgggtagac gacccactga
     4201 tgaaagatcc ctgggagcgc gtcaattcag cggcgatcta ctggggcgcc gatacgctgc
     4261 tgggggcggt ttatatcggc tacggtcgct ccagcctgaa tcaatcgtct ttctacctgg
     4321 tcatcgggcc gaaattttag cactcggcac cgctgtcgac tcagctgcgc ggtctcctgg
     4381 ttaaacaccg ggcaacggca tcgtcctcgc cgcatgcttc gcaactgcgt cgctaatcgg
     4441 ggccgggttc agtctccgcc gctcggcgag gcgattgtga aaccctgacg aaagccttgc
     4501 gcaggcaaag gtgtcgcctg ctgccgggtc accgtcgcag gtgttacgcg gatatcgagc
     4561 cgccgccgat ttctcgccga atcggatcgg cgcgatgcgc ctcgcccatg cgcgacaaat
     4621 cccgccaatc gcctgaaaaa caataacttg ctatcgccgg gcccttaaat gatatgattg
     4681 cgcgattatg cagaatttcg taaccgcgtg aaacacagag aggcgagctc agaccaccta
     4741 gacgtcatcc cccgcagcca gcacaacatc tcccgcaaat cgatttccga ttccgcgttg
     4801 aaggtgttgt accgtctgaa caagtcgggc catcgagcct gcctggtcgg cggtggggtg
     4861 cgcgatctgc tgctcggcat acaacccaag gacttcgacg tcgccaccga tgcgacccc
//
LOCUS       DATCZV010000071         3340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_428965, whole genome shotgun sequence.
ACCESSION   DATCZV010000071 DATCZV010000000
VERSION     DATCZV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428965"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VIW27_01625"
     CDS             complement(<1..1273)
                     /locus_tag="VIW27_01625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5738389.1"
                     /translation="MNSTADRFTTDDHGWAELLPAREPLPALAVDRRVPWTVVGAGLT
                     GLACARRLAELHPDDEILLLEARRVAQGASGRNSGFVVATSQFPGPFDARQAEHYRRI
                     NRINQAGLELLSAQVDKLGIDCQWQREGFIHTAADHRALDEYRHFRHYLQTLEVEHRL
                     LSADELRELLGTGLYRAGIRIGDGALVQPAALVYGLADSLPANVSLHERSPLLKIERG
                     SPLTLRLADARVTTDRLILAVNYEAPGLGFLHRRLVGSTLAGSFTRRLSADELASLGS
                     LDQWGAISLHGGGATLRLTRDARISIRNTAEYRGAALLTDAELTRRQQIHRVAFERRF
                     PQLAGVAFEYAWSGVEGITRNGTNFFGRQHENVFLVGGYNGSGVSRGTAFGTAIADYA
                     SGATTQTVSDCLASPRAASIPPRPLLDIGAFF"
     gene            complement(1326..2375)
                     /locus_tag="VIW27_01630"
     CDS             complement(1326..2375)
                     /locus_tag="VIW27_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY5738390.1"
                     /translation="MKWLQALTALPNRIRALKNWRGFRTLSALRFGGGLQTALAAWRE
                     RRRKPLPDDQTHTFVARTYPGSRTRRYLVHVPRSKLNARARPLVVVLHGCRQDHRDIE
                     QITGFNELADRHGFLVAYPFVTSYSGMRNKNCWGWWFDNEIHAGAGEVEDLWQIVEEI
                     RRDYPVDARRIHVTGLSSGAGMAVAMMVAHADKIASGAAVAGMPYAERPEAVRHAFNE
                     EPRYRPVVAVVTAMRAEMGDRARRVPIQIVHSHADDKVDIRAAELLRDSWGHCFDIDT
                     REAFKVSSGTTGSTAWEHRRYRDDSRESVLETLFLEGPGHGWYGGNPGNFSFPDAPDI
                     KRDIWRFFVSHPLPR"
     gene            2834..>3340
                     /locus_tag="VIW27_01635"
     CDS             2834..>3340
                     /locus_tag="VIW27_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5738391.1"
                     /translation="MRFSAHNWVAAGLLLPALVSAAGDLSTLQVSKVTMPLEFEVDGV
                     VEARRRATLSAEVGGTVENIYFDVDDRVEQGAVVLRIRDREYRARLQQAQAALDEAEA
                     SLRQVELEFERSRDLRRQKLIPQADFDKASANLAGAQARRESARANLDEAREQLQHTV
                     VEAPYSGVV"
BASE COUNT          570 a         1018 c         1167 g          585 t
ORIGIN      
        1 tgaagaaggc gccgatgtcg agcagcgggc gcggtggaat cgaagccgcg cgaggcgacg
       61 ccagacagtc gctgaccgtc tgcgtggtcg cgccgctggc ataatcggcg atcgcggtgc
      121 cgaaggcggt gccgcggctg acgccggatc cattgtagcc cccgacgaga aatacgtttt
      181 cgtgttggcg gccgaaaaaa ttggtgccgt tgcgggtgat gccctcgacg ccggaccagg
      241 cgtattcgaa agctaccccg gccaactgcg gaaatcgccg ctcgaaggcc acacggtgga
      301 tctgctggcg gcgcgtgagt tcggcatcgg tgagcagcgc cgcgccgcgg tactcggctg
      361 tgttgcgaat gctgatgcgg gcgtcgcggg tcagtctcag ggtcgcgcca ccgccgtgca
      421 gcgagatggc cccccattga tcgaggctgc cgagcgatgc cagttcgtcg gcgctcaggc
      481 ggcgcgtgaa gctgccggcg agcgtgctgc cgaccagccg tcggtgcagg aagccgagac
      541 ccggcgcctc gtaatttact gccagtatca ggcggtcggt ggtcaccctg gcatcggcga
      601 gacgcagggt gagcggcgag ccgcgctcga ttttgagcag cgggctgcgt tcgtgcaggc
      661 tgacattggc gggcagggag tcggccagcc cgtagaccag cgcggccggc tgtaccagcg
      721 cgccgtcgcc gattcgaatt cccgcccggt agagcccggt acccagcagc tcgcgcagct
      781 cgtcggcgga cagaagccga tgctcgactt cgagcgtttg caggtaatgc cgaaaatggc
      841 ggtactcgtc gagcgcgcgg tggtcggcgg cggtatgaat aaagccctcg cgctgccact
      901 ggcaatcgat accgagcttg tcgacctgcg ccgatagcaa ttccagcccg gcctggttga
      961 tgcggttgat gcgccggtag tgctcggcct ggcgggcgtc gaacggacct ggaaactggc
     1021 tggtagcaac cacgaagccg gaattgcggc ccgacgcgcc ctgtgcgacg cggcgtgcct
     1081 cgagcagcag gatttcgtca tcgggatgca actcggccag ccgccgcgcg caggcaaggc
     1141 cggtgagccc ggcgccgacc acggtccagg gcacgcgccg gtcgacggcg agcgcgggca
     1201 acggctcgcg ggccggcaac agctctgccc agccgtggtc gtcggtggtg aaacggtcgg
     1261 cggtcgagtt catgctgcgg cgtgcctgcg tgcgaggata ctgggatcct cgaatcatac
     1321 cgcgtttatc gcggcagggg atgggaaacg aagaaacgcc agatgtcgcg cttgatgtcg
     1381 ggggcgtcgg gaaagctgaa gttgccgggg ttgccgccgt accagccgtg cccggggcct
     1441 tcgagaaaca gggtttcgag caccgattcg cggctgtcgt cgcggtagcg gcggtgttcc
     1501 caggcagtgc tgccggtggt gccgctactg accttgaaag cctcgcgggt atcgatgtcg
     1561 aagcagtgtc cccagctgtc gcgcagcagt tcggcggcgc gtatatcgac cttgtcgtcg
     1621 gcgtgagagt gcacgatctg gatgggtacg cggcgagcgc gatcgcccat ttccgcccgc
     1681 atcgcggtta ccacggcgac caccggccgg tagcggggtt cctcgttgaa ggcatgacga
     1741 accgcctcgg ggcgttcggc gtagggcatg ccggcgaccg ccgcgccgga ggcgatcttg
     1801 tcggcatggg cgaccatcat cgccaccgcc atgccggcgc cggacgacag gccggtgacg
     1861 tgaatgcgcc gtgcgtcgac cggatagtcg cgacgaattt cctcgacgat ttgccacagg
     1921 tcctcgacct cgccggcacc ggcgtggatt tcgttatcga accaccaacc ccagcaattc
     1981 ttgttgcgca tgccgctgta gctggtgacg aacggatagg cgaccagaaa gccatggcga
     2041 tccgccagct cgttgaagcc ggtaatctgt tcgatgtcgc ggtggtcctg gcgacagcca
     2101 tgcagcacca ctaccagcgg acgggcgcgc gcgtttagct tgctgcgcgg cacgtgcacc
     2161 aggtagcggc gcgtgcgcga tccgggatag gtacgggcga cgaaagtatg ggtttgatcg
     2221 tcgggcagcg gcttgcggcg gcgctcccgc caggcagcca gtgccgtctg caggccgccg
     2281 ccaaaccgca gcgcggacag cgtcctgaat ccgcgccagt ttttcagtgc gcggatacgg
     2341 ttcggcaaag ccgtcaatgc ctggagccat ttcatcgggt ttttttgtca ccatagacgc
     2401 gtctggacgc gcgggatttg tcactaacgc aagcatagtc gactttttgc gcagtgcagc
     2461 aatctcgacg tatttttcgc cgtccgccgt attgtcgaga tcgccggcaa atcatgcgtc
     2521 gctgcgcgga agccgctggc gaggcgcccg aggccgcttc tatccctacc aggtcaccgg
     2581 ctgcaggggt ttgcggcaag ccggcaatga ccaagacgag ttgcgggccg ggaatcgtcg
     2641 cttccccaat cggccggcgg acgctctatt cgagtgcgcc cgcggcgact cgcgaagctg
     2701 cagccaagtc acaaggtttt gcccgatatc aattgtttcg gcggcgcagg gaatactatg
     2761 tcaactggga tatgttatat tagaaaattc taatatatgg ttgcgtctcg gtttgggcgc
     2821 gcaggagcgt tgcatgcgtt tttcagcaca taactgggta gcggccggcc tgctactgcc
     2881 cgcgctggtc tcggcggccg gcgatctgtc gacgttgcag gtcagcaagg tgacgatgcc
     2941 gctcgagttc gaggtcgatg gcgtggtcga ggcgcgccgc cgagcaaccc tgtccgccga
     3001 ggtcggcggg acggtggaaa atatctattt tgacgtcgat gaccgcgtcg aacagggcgc
     3061 ggtggtgctc aggattcgcg accgcgaata ccgcgcgcga ctgcagcagg cacaggcggc
     3121 gctcgacgag gccgaggcca gcctgcgcca ggtggagctg gaattcgagc gcagccggga
     3181 tctgcgccgc cagaaactga ttccacaggc cgacttcgac aaggccagcg ccaacctggc
     3241 cggtgcgcag gcgcgtcgcg agtcggcgcg cgccaatctc gacgaggcgc gagagcaatt
     3301 gcaacatacc gtcgtcgagg cgccctatag cggcgtcgtc
//
LOCUS       DATCZV010000072         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_640374, whole genome shotgun sequence.
ACCESSION   DATCZV010000072 DATCZV010000000
VERSION     DATCZV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640374"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="mraY"
                     /locus_tag="VIW27_01640"
     CDS             complement(<1..684)
                     /gene="mraY"
                     /locus_tag="VIW27_01640"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY5738392.1"
                     /translation="MLYSLSEYLTQYYSGFNVFGYLTMRTILGVLTALLISLIVGPVM
                     INYLGSYNIGQSVRNEGPESHFSKAGTPTMGGALILVAIVTSTLLWSDLSSRFVWIVL
                     FVTVGFGAVGWVDDYKKIVFGNSKGLSARAKYFWQSVIGLGAALALFYSAQYPIETQL
                     IVPFLKNILIDLGWLYVVLTYFVIVGSSNAVNLTDGLDGLAILPTVLVGGALGVFAYL
                     TGNFNFSNYL"
     gene            complement(674..2044)
                     /gene="murF"
                     /locus_tag="VIW27_01645"
     CDS             complement(674..2044)
                     /gene="murF"
                     /locus_tag="VIW27_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5738393.1"
                     /translation="MISLDLQTLASILGASSSGLSAAEFTGVAIDSRQSCAGRLFVAL
                     RGENFDGHGFVDAARQGGAVAALVESRQDCAIEQIEVDDCRLAMAQLANYWRRHCDPC
                     VLALTGSNGKTTVKEMLYRILAAQAPTRASRGNLNNDIGVPLTLFELGRDHDFAIVEM
                     GANHRGEIARLAEIAEPDIVYVNNAAASHLEGFGDLQGVIEAKGELYAYCRPEQRAVF
                     NLDEPASAHWRSICAAERQLTCALAQPADVGAHCTLAGNSLKIEFVYGQQTRSCELGV
                     LGEHNARNALAAVSMALMAGIDLPAAVDALHGFSGVGGRQQILPGPAGSRIIDDSYNA
                     NPASLEAGIRVLCALDGAAWLALGDMAELGPEAEALHRQAAHSARDLGIERLFGIGEM
                     SCIASREFGKFGHCYEHIDDMANAILAQIHPGVNLLVKGSRSAGMERLVALLSAARET
                     GEANAV"
BASE COUNT          397 a          677 c          649 g          364 t
ORIGIN      
        1 caggtagttg gaaaaattga agttcccggt caggtaggcg aataccccca gcgcgccgcc
       61 gaccagcacc gtcggcagga tcgcgaggcc atcgagacca tcggtgaggt tgaccgcgtt
      121 gctgctgccg acgatgacga aataagtcag caccacgtaa agccagccga gatcgatgag
      181 gatgtttttc agaaacggca cgatcagctg ggtttcgatc gggtattgtg cgctgtaaaa
      241 cagcgccagc gccgcaccca ggccgatcac cgattgccag aagtacttgg cgcgcgccga
      301 cagccccttg gaattgccga agacgatctt cttgtagtcg tcaacccagc cgacggctcc
      361 gaaaccgacg gtcacgaaca gcacgatcca gacgaagcgc gacgacaggt cgctccacaa
      421 cagcgtgctg gtgacgatcg cgacgaggat cagcgcgccg cccatggtcg gcgtgccggc
      481 cttcgagaaa tggctctcgg gaccctcgtt gcgcacgctt tggccgatgt tgtagctgcc
      541 gagataattg atcatcaccg ggccgacgat cagcgagatc aacagcgcgg tcagcacgcc
      601 gagaatcgtg cgcatggtca ggtaaccgaa cacgttgaag ccgctgtaat actgcgtgag
      661 gtattccgac aggctataca gcattggctt ctccggtttc gcgggctgcg ctcagcagcg
      721 ccaccagccg ctccatgccg gccgagcgcg agcccttgac cagcagattc acgccggggt
      781 gaatctgcgc cagtatggca ttcgccatat catcgatgtg ctcgtagcaa tgcccgaatt
      841 taccgaactc gcggctggcg atacagctca tttcgccgat gccgaaaagc cgctcgatgc
      901 cgagatcgcg cgcgctgtgc gccgcctggc gatgcaatgc ctcggcctcg ggtcccagct
      961 cggccatgtc gccgagcgcc agccaggccg cgccgtcgag tgcgcacaac acgcgtatgc
     1021 cggcttccag cgaggccgga ttggcgttgt aggaatcatc gatgatgcgg ctgccggcgg
     1081 gacccggcag aatctgctga cggccgccga cgccgctgaa tccgtgcagc gcgtcgaccg
     1141 ccgcgggcag atcgataccg gccatcaatg ccatcgacac cgccgccagc gcgttgcgcg
     1201 cattgtgctc gccgagcacg ccgagctcgc agctgcgggt ctgttggccg taaacgaact
     1261 cgattttcag actgttgccg gccaatgtgc agtgcgcgcc gacatctgcc ggctgcgcca
     1321 gcgcacaagt cagttgccgc tcggcagcgc agatactgcg ccagtgcgcg ctggccggtt
     1381 cgtcgaggtt aaacaccgcg cgttgctcgg gtcggcaata ggcatagagc tcgcccttgg
     1441 cctcgatgac cccctgcaga tcgccgaatc cctcgaggtg cgatgccgcc gcattattga
     1501 cgtagacgat gtcgggctcg gcgatctcgg caagccgcgc gatctcgccg cgatggttgg
     1561 cgcccatctc gacgatcgcg aaatcgtggt cgcgaccgag ctcgaacagc gttaggggca
     1621 cgccgatatc gttgttgaga ttgccgcgcg aggcccgcgt cggcgcctgc gcggcgagaa
     1681 tgcggtaaag catttccttg accgtggtct tgccgttgct gccggtcagg gccagcacgc
     1741 acgggtcgca gtgccggcgc cagtaattgg ccagctgcgc catcgccagg cgacagtcgt
     1801 caacctcgat ctgctcgatg gcgcaatcct gccggctctc gaccaaggct gcgaccgccc
     1861 cgccctgccg ggccgcgtcg acgaagccgt ggccgtcgaa attctcgcca cgcagggcaa
     1921 caaacagtcg ccctgcgcag gattggcggc tatcgatggc gacgccggta aattcggcgg
     1981 ccgacaagcc gctgctactc gcccccagga tcgacgccag tgtctgcagg tcgagactaa
     2041 tcacctttta cctgcaatat gcgcctgacc acgtggcgat cgctgaa
//
LOCUS       DATCZV010000073         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_429149, whole genome shotgun sequence.
ACCESSION   DATCZV010000073 DATCZV010000000
VERSION     DATCZV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429149"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..527)
                     /locus_tag="VIW27_01650"
     tRNA            complement(452..527)
                     /locus_tag="VIW27_01650"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Lys,seq:ttt)
     gene            complement(655..1623)
                     /gene="ybgF"
                     /locus_tag="VIW27_01655"
     CDS             complement(655..1623)
                     /gene="ybgF"
                     /locus_tag="VIW27_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY5738394.1"
                     /translation="MKIASSPLPAIVLVLVTASTGPGMVHAQTALTPAQLTLRVERLT
                     ELSQRSEEKLGTIQEQLMLLSEEMSRMGRLVDNRAVIDMIQQVDQLSGEISQLTGAIE
                     EQRFEIESIKKRQRELYADIDRRLQNLESGSAARAAAGSITLPPSDTGASASQQAAAA
                     AAASSAAAAGVSSSQPSSAGSEVSASLTPPAPPTAEEKAAYQAAFDTLKEGRYKNAKT
                     ELRAFLDRYPNSSYAGNAQYWLGEAHYVTRSFQQGIVEFSNVLEKYPTSNKVPDAMLK
                     LGYTYYELQQYPQARQILTDLRDKFPGETAANLAAKRLERIRKEGH"
     gene            complement(1625..2233)
                     /gene="pal"
                     /locus_tag="VIW27_01660"
     CDS             complement(1625..2233)
                     /gene="pal"
                     /locus_tag="VIW27_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY5738395.1"
                     /translation="MKNPTFCKPWMIAAVLALFVSACATTQDEIIEDGPPTVDESATD
                     GTTSGAETTDGAAASGLEGSGDSGATAIADSEREMTAAEMLEQSDGPLANRTIYFEYD
                     SASLTDESIAILETHGNFIAANGEVRVRLEGHADERGSREYNIGLGDRRAQSVRRVLL
                     FQGAATNQIDTVSYGEERPVDSGHSEEAWAKNRRVELIYTVN"
     gene            complement(2264..>2835)
                     /locus_tag="VIW27_01665"
     CDS             complement(2264..>2835)
                     /locus_tag="VIW27_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEY5738396.1"
                     /translation="GVNSAPSWSPDGKFLALVLSKDGSPDIYIMNTATRRLQQLTRHR
                     SIDTEPVWTPDGRSVIFTSDRSGSPQLYQIAIDGGQPRRLTFEGNYNSAPNLSPDGRY
                     LAMVHAMRGQYRIAQLELETGNLTILTDSSLDESPSFSPNGKMVLYASTQGPNGILYA
                     VSIDGRAKHKLSDQAGDIREPVWGPFESK"
BASE COUNT          472 a          854 c          916 g          593 t
ORIGIN      
        1 atccgcaagg atttcaggtt aattgatcgg ggagaaaaat ggtgggccgt gagcgattgc
       61 gcagcacatc cctgtgccgc gcccctacgg ctggctgcgc cagccttcag ggccgccctg
      121 cgggcgtcca aatgcgctcc cggcgcattt gtcgaacgct cgaccacgtg acaccccgga
      181 atcaggtggt cgagctctgg ctcgccaacc gatgtggctt caaattaaat taatgggaaa
      241 aaatggtggg ccgtgagcga ttgcgccgca catccctgtg cggcgcccct ccggacggct
      301 gcgccgtcct gcggggccag cgctgcgcgc tgtccaaatt tgctccgggc aaatttgtcg
      361 aacgctcgac cacgtgacac ctcggaatca ggtggtcgag ctgtagctcg ccatccgcaa
      421 ggatttcagg ttaattgatc ggggagaaaa atggtgggcc gtgagcgatt cgaacgctcg
      481 accacctgat taaaagtcag atgctctacc aactgagcta acggcccccg ggaagaaccg
      541 ggaatgatac ccatccccgg ttcgatttca aggcaaatct ggtcgcgacc cctgttgtac
      601 ggccgaaatt ccaccgcggt atccgcccgg tggcggacgc ggcggccaga cgggttagtg
      661 tccctccttg cgaatccgct cgaggcgctt ggctgccagg ttggcggcgg tctcgccggg
      721 aaacttgtca cgcaagtcag tcagtatctg cctggcctgc gggtactgct gcagctcgta
      781 ataggtgtat ccgagcttca gcatcgcgtc cggtaccttg ttgctggtcg ggtatttttc
      841 cagcacgttg ctgaactcga cgatgccctg ctggaagctg cgcgtgacgt agtgcgcctc
      901 gccgagccag tactgcgcat tgccggcata actgctgttc gggtagcggt cgagaaacgc
      961 gcgcagttcg gttttggcat tcttgtagcg accctccttg agggtatcga aggcggcctg
     1021 gtaggcggct ttttcctccg cggtcggcgg cgctggcggc gttagcgacg cgctgacctc
     1081 acttcctgcg gacgacggct ggctagagga aacgcccgcc gcggcggcac tcgacgcggc
     1141 ggcggccgcg gcggcctgct ggctggcgct ggcgccggta tcgctcggcg gcagggtaat
     1201 cgacccggcc gccgcgcgcg ccgcggaacc gctctcgaga ttctgcaagc ggcgatcgat
     1261 atcggcgtac agctcgcgct ggcgcttctt gatgctctcg atttcgaatc gttgctcctc
     1321 gatagcgccg gtcagctggc tgatttcgcc ggataactga tcgacctgct ggatcatgtc
     1381 gatgaccgca cgattgtcga ccaggcgtcc catgcgcgac atttcctcgg acaacagcat
     1441 cagttgctcc tggatggtgc cgagcttttc ctcggaacgc tgcgacagct cggtcaggcg
     1501 ctccacacgc agggtcaatt gcgccggcgt cagcgcggtc tgcgcgtgga ccatgcccgg
     1561 cccggtcgaa gccgtaacca ggacgagcac aatggcaggc agcgggctac tggcgatctt
     1621 catctcagtt caccgtgtaa atcagctcga cacggcggtt tttggcccag gcttcctcgc
     1681 tgtggccgct gtccaccggg cgttcctcgc cgtagctgac ggtatcgatc tggttggttg
     1741 cggcgccctg gaacagcagc acgcggcgta ccgactgcgc gcggcgatcg ccgaggccga
     1801 tgttgtattc acggctgcca cgctcgtcgg catgaccctc gaggcgcacc ctgacctcgc
     1861 cgttggcggc gatgaagttg ccgtgtgttt cgaggatcgc tatcgattcg tcggtcagcg
     1921 aggcactgtc gtattcgaag tagatggtgc ggttggcgag cggaccatcg ctctgctcga
     1981 gcatctcggc ggcagtcatt tcacgttcgc tgtcggcgat cgcggtggcg ccgctgtcgc
     2041 cgctgccctc gagaccgctg gccgcggcgc catcggtggt ttcagcgccg ctggtggtgc
     2101 cgtcggtcgc cgactcgtcg acggtcggcg ggccatcctc gatgatttcg tcctgcgtgg
     2161 tggcacaggc ggagacgaac agggccagca ccgcggcgat catccagggt ttgcaaaaag
     2221 tggggttttt catgtgaata ttagctccgt ttgaaatggg atattacttg ctttcgaatg
     2281 gtccccagac cggttcacgt atatcgccgg cctggtccga taacttgtgc ttggcacgcc
     2341 cgtcgatcga taccgcgtaa agaatgccgt tggggccctg cgtggacgcg tacagcacca
     2401 tcttgccgtt gggcgaaaac gacggcgatt catcgaggga actgtcggtg agtatcgtca
     2461 ggttgccggt ttcgagttcg agttgcgcaa ttctatattg cccgcgcatc gcgtgcacca
     2521 tggcgagata acggccgtcc ggcgacaggt tcggcgcact gttgtagttg ccttcgaagg
     2581 tcaaacgcct cggctgaccg ccgtcaatag cgatctggta caactgcggc gagcccgagc
     2641 ggtcggaggt aaagatgacc gaacgcccgt ccggtgtcca caccggttcg gtatcgatgc
     2701 tgcgatggcg ggtcagttgc tgcagccgcc gggtggcggt gttcatgatg tagatgtcgg
     2761 ggctgccgtc cttggaaagc accaacgcca ggaacttgcc gtccggcgac cagctcggcg
     2821 cgctgttgac gcctt
//
LOCUS       DATCZV010000074         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_375990, whole genome shotgun sequence.
ACCESSION   DATCZV010000074 DATCZV010000000
VERSION     DATCZV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375990"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIW27_01670"
     CDS             <1..193
                     /locus_tag="VIW27_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009804896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738397.1"
                     /translation="LLETYFGNYTRLVYLAQTDSDELTAQARAAADQLGLRFERLYTG
                     MGEMVPALDAAMQEALWRS"
     gene            181..513
                     /locus_tag="VIW27_01675"
     CDS             181..513
                     /locus_tag="VIW27_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY5738398.1"
                     /translation="MAQLKIVFWRDIPGQVVIRKGRRSTRLRLPARFTKAIERASYRL
                     KKTQQDAMFEPWHDVSQPFEGDVEQQARQLLRRLETQYTDEVLETLIRASGVDENRSL
                     NSELDRHV"
     gene            506..976
                     /locus_tag="VIW27_01680"
     CDS             506..976
                     /locus_tag="VIW27_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5738399.1"
                     /translation="MYKFRQWSVTNSRRLEAVYRKFEPLLIRLHPLFKLLGYGRLEKP
                     VRIVEKAVKGLMFDCQMCGKCALSSTGMSCPMNCPKSIRNGPCGGVRMDGHCEVIPQM
                     RCVWVEAWRGSQQMRDNQAIQQVQIPVNNLLKGSSSWLRVVRHEYAAHEGERIQ"
     gene            973..1983
                     /locus_tag="VIW27_01685"
     CDS             973..1983
                     /locus_tag="VIW27_01685"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5738400.1"
                     /translation="MTGAQFEQNPGGPLLPKEGHVSRGRLERVLRSGKFAVTAELAPP
                     DSAMADDVYERARMFDGYVDGMNATDGSGAHCHMSSVAMCALLTNVGYSPVLQISCRD
                     KNRIAIQGDVLGAAALGVSNVLCLSGDGVQVGDHPEAKPVFDLDSMSLLETIRIMRDE
                     ARFLSGRPITTPPEMFLGAAENPSAPPFDMRPHRLAKKIAAGAQFIQTQYVFDIEHLR
                     QFMARSRDLGLLEKVFILPGVGPLASARTALWIRNNVPGIHIPDAIIKRLEGASEPKQ
                     EGRRICVELIQQISEIEGVSGVHIMAYRQEEAVGEIVQQSGVLDGRVPWYPGCELQSN
                     SA"
     gene            2032..>2790
                     /locus_tag="VIW27_01690"
     CDS             2032..>2790
                     /locus_tag="VIW27_01690"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5738401.1"
                     /translation="MTETVVSSATRELVIGFDRPFVIIGERINPTGRKIMAEEMKNGD
                     YSRVQSDALAQVEAGAHMLDVNAGIPLADEPRILAEAVQLVQSLTDVPLSIDSSIIDA
                     LEAGLSVYQGKALVNSVTGEDESLERVLPLVKKHGAAVVAISNDETGISEDIDVRYNV
                     AKKIVERAADHGIPACDVVVDPLVMPIGAINTAGREVLKLIHRLRTELKVNTTCGASN
                     ISFGLPNRNGFNGAFISMAIQAGMTSAITNPLHAE"
BASE COUNT          568 a          821 c          877 g          524 t
ORIGIN      
        1 attgctggaa acttatttcg gcaattacac gcgcctggtt tacctggcgc aaaccgactc
       61 cgatgagctt accgcgcagg cgcgcgcggc ggccgatcag ctcgggctgc gcttcgagcg
      121 gctatacacc ggcatgggcg aaatggtgcc ggcgctggac gcggcgatgc aggaggcatt
      181 gtggcgcagt tgaagatagt gttctggcgc gacatcccgg gacaggtcgt gattcggaaa
      241 ggccgtcgca gcactcgtct gcgcctgccg gcgcgtttca cgaaggcgat cgagcgcgct
      301 tcgtatcggc tcaagaaaac tcagcaggac gcgatgttcg agccctggca cgacgttagc
      361 cagccgttcg aaggtgacgt cgagcagcag gcgcggcaac tgttgcggcg actggaaacc
      421 cagtacacgg acgaagtgct ggaaactttg attcgagcca gcggtgtcga cgaaaaccgc
      481 agcctcaact ctgaactgga tcgacatgta taaatttcga caatggtcgg taacgaactc
      541 gcgcaggctc gaagccgttt atcgcaaatt cgagccgttg ctgatcaggc tacacccact
      601 gttcaaactg ctgggctacg ggcgtctcga aaaaccggtg cgaatcgtcg aaaaagccgt
      661 caagggtctg atgttcgatt gccagatgtg cggcaagtgc gcgctcagtt ccaccggcat
      721 gtcctgcccg atgaattgcc ccaagtcgat ccgcaacggt ccctgtggcg gcgtgcgcat
      781 ggacggtcac tgcgaggtca taccgcaaat gcgctgtgtc tgggtcgagg cctggcgcgg
      841 cagccagcag atgcgggaca atcaggcgat tcagcaggta cagattcctg tcaacaatct
      901 gctcaagggc agctcttcct ggctgcgcgt ggtgcgccac gaatatgccg cgcatgaagg
      961 cgagcgcatc caatgactgg cgcccagttc gagcagaatc cgggaggccc gttgctgccg
     1021 aaagaagggc acgtctcgcg cgggcgcctc gagcgcgtgt tgcgctccgg caagttcgcg
     1081 gtaaccgctg aactggcgcc gccggattcg gccatggccg atgacgttta tgaacgcgcg
     1141 cgtatgttcg acggctatgt cgacggcatg aacgcaaccg acggctcggg tgcccattgc
     1201 cacatgtcca gcgtcgcgat gtgcgcgctg ttgaccaacg tcggctattc cccggtgctg
     1261 caaatttcct gccgcgacaa gaaccgcatc gcgatccagg gtgacgtgct cggcgccgcg
     1321 gcgctgggcg tttccaatgt gctctgcctg agcggtgacg gcgtgcaggt gggtgaccat
     1381 ccggaagcca agccggtgtt cgatctcgac agcatgtcgc tgctcgaaac cattcgcatc
     1441 atgcgtgacg aagcgcgttt cctgagtggt cggccgatta ccacgccgcc ggaaatgttt
     1501 ctcggcgccg ccgaaaatcc gagtgcgccg ccgttcgaca tgcgccccca tcggctggcc
     1561 aagaagattg ccgccggcgc ccagttcatc cagacgcaat atgtcttcga tatcgagcat
     1621 ctgcgccagt tcatggcgcg ctcgcgcgac ctgggattac tggagaaggt cttcatcctt
     1681 cccggtgtcg gtccgctggc gtcggcgcgc accgcgctgt ggatacgcaa caacgtgccc
     1741 ggtatccata tcccggacgc gattatcaag cgcctggaag gcgccagcga accgaagcag
     1801 gaagggcgca gaatctgtgt cgagctgatc cagcagatca gcgagatcga gggcgtcagc
     1861 ggcgtgcaca taatggccta tcgccaggaa gaggccgtgg gtgaaatcgt tcagcaatcc
     1921 ggggtgctcg acgggcgggt gccgtggtac ccgggttgcg agttacagtc caattcggcc
     1981 tgacgggcgt atattggttt tagctaagta atagttgtag aggatataac aatgactgaa
     2041 acggtcgtca gttccgccac cagagaacta gttatcggct tcgatcgccc gttcgtaatc
     2101 atcggcgagc gtatcaatcc cactggccgc aagatcatgg ccgaggagat gaaaaacggc
     2161 gactatagcc gggtgcaaag cgacgcgctg gcgcaggtcg aggccggggc gcatatgctc
     2221 gacgtcaacg caggtatccc gctggcggac gagccgcgta tcctcgctga agcggtgcag
     2281 ctggtgcaga gcctgaccga cgttccgcta tctatcgact cgtcgattat cgacgcgctc
     2341 gaagccgggc tttcggtata ccagggcaag gcgctggtga actcggttac cggtgaggac
     2401 gaaagcctcg agcgggtgct gccactggtc aagaagcacg gcgccgcggt ggtcgcgata
     2461 tcgaacgacg aaaccggtat ttctgaagac atcgacgtcc gctataacgt ggccaagaaa
     2521 atcgtcgagc gcgccgccga ccacggcatc ccggcctgcg acgtggtggt cgatccgctg
     2581 gtcatgccca tcggtgcgat caatactgcg gggcgcgaag tgctcaagct gattcaccgc
     2641 ctgcgcaccg agctcaaagt caacaccacc tgcggcgctt ccaacatcag tttcggactg
     2701 cccaaccgga acggcttcaa cggcgctttc atcagcatgg caatccaggc cggcatgact
     2761 tcggcgatta ccaatccgct gcatgccgag
//
LOCUS       DATCZV010000075         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_164155, whole genome shotgun sequence.
ACCESSION   DATCZV010000075 DATCZV010000000
VERSION     DATCZV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164155"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VIW27_01695"
     CDS             complement(<1..827)
                     /locus_tag="VIW27_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5738402.1"
                     /translation="MALLSAPQVERFERDGYLLLSDVVPAGVLRAMRREFEQWKESSR
                     QFSGPYGETMDGRPRFDVEPGHSAARPALRRIASPIEISETYLEFMRDNRALDAVVDI
                     LGPNIRFENAKINSKQPGTATRVRYHQDFLFEPHTNDDMVTVLFFIDEVTDANGPLEV
                     VPGSHKGPLHDHWHEGVFTGAIADEIADRMKARAVACTGAAGSACLMHTRVVHGSAPN
                     LADRPRTLFICEYTAEDAYPLQVNHIPSEYMYEIVRGEYSGRVRCSDYQMLFPEVPTG
                     "
     gene            complement(1025..1972)
                     /locus_tag="VIW27_01700"
     CDS             complement(1025..1972)
                     /locus_tag="VIW27_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5738403.1"
                     /translation="MPADFPPRLDNKFYLTEAGVETEVMYKWGLELPEFAMFPLLDDP
                     EADEVIRNMYRRYFEVAAEHQTGLLLNGHDYRASPDWAAKLGYDADGLRQMQHRVIRF
                     LDEMRAEYADRVSDVYIAACLGPRGDAYGTGGEITADEAEEYHTVQLQNLEGTGADMA
                     VAATFNNIPEAIGVIRAANNLGIPLGVSLTLTPEGRLRSGPSLREAVETIDEATGGVA
                     AWFGTNCSHPLEFEAALAEQGQWLERLRYIRPNAARMDKIALCSLGHLEDGDPLELGG
                     QIGEVARRLPRADILGGCCGTDERHLAEIARHVNRVRAA"
     gene            complement(2117..2593)
                     /locus_tag="VIW27_01705"
     CDS             complement(2117..2593)
                     /locus_tag="VIW27_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004727979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HEY5738404.1"
                     /translation="MNIFILDENIEQCARYHCDQHVVKMILESVQMLCTALNKKGFTT
                     PYRSTHAKHPCVLWVEDSYDNFCWLRSLALALNAEYRYRFERSADHKSIAVLDEIAPI
                     TYPSRGLTEFAQAMPVEYKVPGDAVAAYRRFYVGEKLGFAKWTRREVPAWVAGAVS"
BASE COUNT          483 a          815 c          816 g          498 t
ORIGIN      
        1 cccgtcggta cttcgggaaa aagcatctgg tagtcgctgc agcgtacccg cccgctatac
       61 tcgccgcgca cgatttcgta catgtactcg ctgggaatat ggttgacctg caatgggtag
      121 gcgtcttctg ccgtgtactc gcagatgaac agggtacgcg gtcggtcggc gaggttgggc
      181 gccgaaccgt gcacgacgcg ggtatgcatc aggcaggccg agccggcggc tccggtacag
      241 gcgacagcgc gagccttcat ccgatcggca atctcgtcgg ctatcgcgcc ggtgaacacg
      301 ccttcgtgcc agtgatcgtg aagcggtccc ttgtgcgacc cgggcacgac ttcgagcggc
      361 ccgtttgcat cggtgacctc gtcgataaag aacagcaccg ttaccatgtc gtcgttggtg
      421 tgcggctcga acaggaaatc ctggtgatac ctgacccggg tggcggtgcc gggttgcttg
      481 gagttgatct tggcattctc gaagcggata ttcgggccaa gaatatcgac caccgcatcc
      541 agcgcgcgat tgtcgcgcat gaattcgagg taggtttccg atatctcgat tggcgaagcg
      601 atgcgccgca acgccggccg tgcggccgaa tgaccgggct cgacatcgaa gcgcggtcga
      661 ccatccatag tttcgccata gggtccgctg aattgccggc tggactcttt ccattgctcg
      721 aattcgcgcc gcattgcacg cagcacgccc gccgggacga catcggatag taacaggtag
      781 ccgtcccgct cgaagcgctc gacctggggc gccgataata aggccattcg aacagcttcc
      841 tccgacatcg aacaactggt tataagtgac cccggctcgc tggcggcagc cagctgccgg
      901 ccaacctaac cattctccgc gacttcgtca acggcggacc gctgccggcc ggcgcacgat
      961 tatccagtcg ctgcagcgca cccgccgagg tcgcgccgat gcgctcgcga ccatgtcgtc
     1021 gacttcaagc ggcgcgcacg cggttgacgt ggcgcgcgat ctcggccaga tggcgctcgt
     1081 cggtgccgca gcagccgccc aggatatcgg cgcgcggcaa acgccgcgcg acttcgccga
     1141 tctgtccgcc gagctccagc gggtcgccgt cctcgaggtg gcccagcgaa cacagcgcaa
     1201 ttttatccat tcgtgcggcg ttcggccgta tgtagcgcag gcgctcgagc cactggccct
     1261 gctccgcgag cgcggcttcg aattccagcg gatgcgagca gttggtgccg aaccaggcgg
     1321 caacgccacc ggtggcttcg tcgatggttt ccaccgcctc gcgcagcgac gggccggagc
     1381 gcagccggcc ttccggggtt agcgtcagac tgacgccgag cggaataccg agattgttcg
     1441 ccgcgcgaat cacgccaatc gcctcgggaa tgttgttgaa ggtcgccgct accgccatgt
     1501 cggcgcctgt cccctcgagg ttctgcagct gcacggtgtg gtattcctcg gcctcgtcgg
     1561 cggtgatttc accgccggtg ccataggcat caccgcgcgg accgaggcag gcggcaatgt
     1621 agacgtcgga tacccggtcc gcgtactcgg cacgcatctc gtcgagaaag cgaatcaccc
     1681 gatgctgcat ttgccgcagg ccgtccgcgt cgtagccgag tttggcggcc cagtccggac
     1741 tcgcgcggta atcgtggccg ttgagcagta gtccggtttg gtgctcggcg gcaacttcga
     1801 agtagcggcg gtacatgttg cgaatgactt catcggcctc gggatcgtcg agcagcggaa
     1861 acatcgcgaa ctcgggtaac tcgaggcccc acttgtacat gacctcggtt tccaccccgg
     1921 cttcggtcag gtagaacttg ttgtcgagtc gcggcggaaa atcggctggc atggaaaccc
     1981 ctcgaggatc tgtaattaca acgagttcgc attgtagcca aaaaaacggg ggaaagatta
     2041 cccgtatatc cgcacccggc agtgcttgcc accgaatcga cgccaacggt cagaggctgt
     2101 tgccgctaga agccgatcag gacaccgcgc cggcgaccca cgcaggcacc tcgcggcgcg
     2161 tccacttggc aaacccgagc ttctcgccga cgtagaaacg acggtaggcg gcgaccgcat
     2221 cgccaggcac cttgtattcg acaggcatcg cttgcgcgaa ctcggtcagg ccgcggctcg
     2281 ggtaggtaat cggcgcgatc tcgtcgagta ccgcgatcga cttgtgatcg gcactccgct
     2341 cgaagcgata gcggtactcg gcattgagcg ccagcgccag gctcctgagc cagcagaaat
     2401 tgtcgtacga atcctcgacc cagagcacgc agggatgctt ggcatgcgtc gagcggtagg
     2461 gtgtggtgaa gcccttcttg ttgagcgccg tgcacagcat ctgcacgctt tcgagaatca
     2521 tcttcaccac gtgctggtcg caatggtagc gcgcgcattg ctcgatattt tcgtcgagaa
     2581 taaagatatt cattcggttt acgggaaaat cc
//
LOCUS       DATCZV010000076         3422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_5552, whole genome shotgun sequence.
ACCESSION   DATCZV010000076 DATCZV010000000
VERSION     DATCZV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5552"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VIW27_01710"
     CDS             complement(<1..510)
                     /locus_tag="VIW27_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY5738405.1"
                     /translation="MSSLTAHNDQPSSGNFQIYSHGVTDTGHVRTKNEDSILVLEDEN
                     LWIVADGMGGHHAGDFASQTITKNLSLFKQISSLDDNILLLEENILNSNAIIRNKSYR
                     FGRNATIGSTVVCVYAWEKYLFTLWAGDSRLYRYRDSKLERLTEDHSYVEELVRMGKL
                     DPREAEHHPA"
     gene            692..1381
                     /locus_tag="VIW27_01715"
     CDS             692..1381
                     /locus_tag="VIW27_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738406.1"
                     /translation="MKKLIVLGFSLLFFTTTSHAIDFGVGAKAGLNGLGLDLTVGLTK
                     NVNLRLSAAAIDIDNEEETVTVGDPGAEGDIAAELEFDYGARAAFIDWHVFGGGFRLS
                     AGMYRNTGAVDLSGTLTGDVIVDGTPVAVGDLGTISGEVELADSYQPYLGIGWGRGAG
                     GDGGLSFSVDLGVAVLEPSVSLDATVTATSGLTQAELDDALRGMEQDAEEDLEDLELW
                     PVFAIGLNYAF"
     gene            1446..1730
                     /locus_tag="VIW27_01720"
     CDS             1446..1730
                     /locus_tag="VIW27_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY5738407.1"
                     /translation="MTRMVQCVVLGEQAPGLDRVPYPGELGQRIFDNVSAEGWRQWIE
                     RQTMIINENGLSTIDPASLEILEQHMLGFIFKEGDMGQQPSGFRPPGAKK"
     gene            complement(1731..2894)
                     /locus_tag="VIW27_01725"
     CDS             complement(1731..2894)
                     /locus_tag="VIW27_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5738408.1"
                     /translation="MPILVASNSEHSLVPEIANGITVDLDDPPAALQAIIAAVGSRDV
                     ACVLATDDSCAELASRVAAHFGLRHNLPAAAQLTQRKDLGREALALAGCRTPEFRVID
                     IASAGSTGPGIDYPVVVKPLGLSASRGVIRADDGPQFVAACRRIDALLETIGVSGYPR
                     DHLLVESYIDGAEFAVEGFIVDGELETIAVFDKPEPLTGPFFEETYYVTPSRLPAGQR
                     QQLLDELARCCRAYGLQQGPVHAELRIAADGPVLLELAARTIGGQCGRLIEFSSRQKL
                     EELVIKGMCGMPFERQARAGSAGVLMIPITEAGILQRVEGLTAAMQTRFVEDIEIHIN
                     PGYELVPLPEGASYLGFIFAQAPDFERTYGALRSAHGRLRFVTRPSWKLENLG"
     gene            complement(3031..>3422)
                     /locus_tag="VIW27_01730"
     CDS             complement(3031..>3422)
                     /locus_tag="VIW27_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY5738409.1"
                     /translation="PASGSGVFEPALTVNELEVTASAQEKLHEIISQTDEDVSAIRIY
                     ISGGGCGGLSYGMTFTDGHSDFDTILQFDGFKILVDVVALNYLKGAQIDYIQEVGRER
                     FVFNNVFAEIGGSGVCGGCGAAGGGCT"
BASE COUNT          651 a         1002 c         1069 g          700 t
ORIGIN      
        1 cgccgggtgg tgttcggcct cgcgtggatc cagttttccc attctcacca gttcctcgac
       61 atagctgtgg tcctcggtga gccgttccag cttcgaatcg cggtagcggt acagccggct
      121 gtcgcccgcc cacagggtaa acaggtattt ttcccaggca tacacgcaga caaccgtgct
      181 gccgatcgtc gcattgcgtc cgaagcggta ggatttattg cggatgatgg cgttcgaatt
      241 gagaatgttc tcttcgagca gcagaatatt gtcgtccagt gagctaatct gcttgaacag
      301 gctcagattc ttggtaatcg tctggctggc aaaatcaccg gcgtggtgcc cgcccatccc
      361 gtccgcgact atccacaggt tttcgtcttc gagcaccagg atcgaatctt cgttcttggt
      421 ccttacgtgg cccgtgtcgg taacgccgtg gctgtagatt tgaaagttgc cggaggatgg
      481 ttgatcgtta tgggctgtta agcttgacaa ctcaactcca ttttcctgtt tcaattattt
      541 tggcactaat aacaataata gtaaattaac agcaaaatga tatgtaggac gtgaagaact
      601 gcgcataagc cgatgaaaat tttaatttaa catccgtatt atgtttgacc tataatcgct
      661 tgaacacgtt ggtttgttaa cgggagatcc gatgaaaaaa ttaatagtgc tgggcttcag
      721 tctgttgttt tttaccacta ctagtcatgc gatcgatttc ggtgtgggcg ccaaggcagg
      781 gctaaatggc ctcggtctcg atctgaccgt cggacttacc aagaacgtta acttgcgcct
      841 tagcgccgcc gccatcgata tcgacaacga agaagaaacc gtcaccgtcg gcgatccggg
      901 tgccgagggc gacatcgcgg ccgagctgga attcgattac ggcgccaggg cggcgtttat
      961 cgattggcac gtgttcggcg gcggtttccg actctccgct ggcatgtaca ggaataccgg
     1021 cgcggtcgat ctgagcggta cgctgaccgg cgatgtcatc gtcgatggca cgcccgtggc
     1081 agtgggcgac ctcggcacaa tcagcgggga agtggagctg gccgactctt accagcccta
     1141 tctcggcatc ggctggggcc gcggcgccgg cggtgacggt ggcctgtcat tttcggtcga
     1201 cctcggcgtc gcggtgctgg aacccagcgt cagtctggat gccaccgtca ccgccaccag
     1261 cggcctgacg caggcggaac tggatgacgc gctgcgcggc atggagcagg atgccgagga
     1321 ggatctggaa gatctcgaac tctggccggt attcgcgatc ggtctcaatt acgcgttctg
     1381 atcttttccg agtctgttat catagagagc agccgttcac cggctgcttt tttttggaag
     1441 gtattatgac gcgaatggtg caatgtgtag tgctcggcga acaggccccg gggcttgacc
     1501 gggtgcctta cccgggcgaa ctcggtcagc gcattttcga caacgtatcc gccgagggct
     1561 ggcggcaatg gatcgagcgc cagaccatga taatcaatga aaacggcctg tcgaccatcg
     1621 acccggccag cctcgaaatc ctcgagcagc acatgctcgg gtttattttc aaggaaggcg
     1681 acatgggtca gcagccgtcg ggttttcgtc cgcccggagc gaaaaaataa tcagccgaga
     1741 ttttccagtt tccagctcgg ccgggtgacg aacctcagcc tgccgtgggc gcttctgagc
     1801 gcgccatagg tacgctcgaa atccggcgcc tgcgcgaaga tgaagcccag gtagctggcg
     1861 ccctcgggca gcggcaccaa ctcgtagccc gggttgatgt gaatttcgat gtcttcgacg
     1921 aagcgtgtct gcatggccgc ggtcaagccc tcgacacgtt gcaggatccc ggcctcggtg
     1981 atcgggatca tcagcacgcc agccgagccc gcgcgcgcct gccgctcgaa aggcattccg
     2041 cacatgccct tgatgaccag ctcttcgagc ttttgccgtg acgaaaactc gatcaggcgc
     2101 ccgcactggc cgccgatggt gcgcgccgcc agctccagca gtaccgggcc gtcggcagcg
     2161 atgcgcagtt ccgcgtgcac cgggccctgc tggagcccgt aggcccggca gcagcgggca
     2221 agctcgtcga gcagttgctg ccgctgtccg gcgggtagcc ggctcggtgt aacatagtag
     2281 gtttcctcga agaacgggcc ggtgagcggc tccggtttgt cgaacaccgc gatcgtctcc
     2341 aattcgccat cgacgataaa tccctcgacc gcgaactccg cgccgtcgat gtaggattcc
     2401 accagcaggt gatcgcgcgg gtagccgctg acgccgatgg tctcgagcag ggcgtcgatg
     2461 cgccggcagg ccgccacgaa ctggggcccg tcgtcggcgc gaatcacgcc gcggctcgcc
     2521 gacaggccga gtggcttgac gaccaccgga tagtcgatac cgggaccggt gctgccggcc
     2581 gaggcgatat cgataacgcg gaattcgggg gttctgcagc cagccagtgc cagcgcctcg
     2641 cgccccaggt ccttgcgctg cgtcaattgc gccgccgccg gaagattgtg gcgcaagccg
     2701 aaatgggccg cgacgcggct ggcgagttcg gcgcagctgt cgtcggtcgc cagcacgcag
     2761 gcgacatcgc gcgagccgac ggcggcgatg atggcctgta aggccgcggg cgggtcgtcc
     2821 aggtcgacgg tgatgccgtt ggcgatttcg gggaccagcg agtgttcgct gttggaagcc
     2881 accaggatcg gcatgcgcag cgcgtgtgcc gcctgcagat aggcagcgac gcggtaactg
     2941 ccggaaggcg ctaccagaac gacaaagccc ggcggggagc caggctttgc gcgttgctgt
     3001 cggcggtgcg gaatcagtag gtcacacggc tcaggtgcat ccgccgccgg ccgcgccgca
     3061 gccgccgcag actcccgaac cgccgatctc ggcgaaaacg ttgttgaaca cgaagcgttc
     3121 gcgaccaact tcctgaatgt aatcgatttg tgcgcccttc aggtagttca gcgccaccac
     3181 gtcgacaagg atcttgaaac cgtcgaactg cagtatcgtg tcgaagtcgc tgtgcccgtc
     3241 ggtgaaggtc atgccataag acaggccgcc gcaaccgccg ccgctaatgt agatgcggat
     3301 cgccgagaca tcctcgtcgg tctggctgat gatctcatgg agtttttcct gcgccgaggc
     3361 cgtgacctcg agttcgttga ccgtcaacgc cggttcgaag acgccggagc cactggcggg
     3421 tg
//
LOCUS       DATCZV010000077         4635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_693814, whole genome shotgun sequence.
ACCESSION   DATCZV010000077 DATCZV010000000
VERSION     DATCZV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693814"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VIW27_01735"
     CDS             complement(<1..630)
                     /locus_tag="VIW27_01735"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEY5738410.1"
                     /translation="MSGKHPILSITGSSGAGTTTVKETFQQIFTRENIDACYIEGDAF
                     HRYNRMEMKHAMADAEANGSKHFSHFGPEANLFEELEDVFRQYSETGTGRTRHYVHDD
                     AESERYGVPPGTFTDWEEFAEGTDLLFYEGLHGAVVTDKVDIARHADLLVGVVPIINL
                     EWIQKLHRDKATRGYSTEAVVDTILRRMPDYVNYIVPQFGRTHINFQRVP"
     gene            complement(639..1667)
                     /locus_tag="VIW27_01740"
     CDS             complement(639..1667)
                     /locus_tag="VIW27_01740"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY5738411.1"
                     /translation="MSTGISLATILENWAGADAPRIALAEILKTITRTSAELSKSIAR
                     HELAAVDSAGERNTDGDPVEPLDSFAHQLYVDALCHSPIGFIASEEAETPIVIDRGAR
                     YGVAIDPVDGSSNIETNLSIGAIFSVLEAGVDELTDICPGERQRAAGFVCFGPQTRLV
                     LTWGEGSQVFLLDPANGEFRTIGEPLQIPNGKCEFAINVSNYRFWEPSVRHFIDDCIA
                     GESGNLGHDFNMRWNASLVAEAYRILMRGGVFLYPGDSRPGYADGRLRLLYEAVPMAM
                     IVEQAGGSASDGRERILDLRLESLHRRVPLVFGSRDRVAEVFDYVSGTAFQATRFPLF
                     EQRGLLRN"
     gene            1751..2683
                     /locus_tag="VIW27_01745"
     CDS             1751..2683
                     /locus_tag="VIW27_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738412.1"
                     /translation="MKEKLYPWLRQLTLRQMRGFEAVVDSGSVSAAARSLHLTPPAVS
                     LQLRDLENAIGLPLLERGEDGLVTTRVGQELLEMSREFQASLDAFAERIDELKGVDHG
                     VVSVGVVSTARYFAPMVLAEFLKLYPEVRIQLQVGNRDATLAKLERLELDFAITGSPP
                     EHFEVKREYIGKNPQILIAPPDHPLANKARIPLSGLRGQTFLLREPGSGTRAVADRLF
                     ARDRELPGNALEFGSNETIKQAVMAGLGIALISAHTVAAELDEKRLVALNVRGLPIHR
                     KWFLVKHAGKRFLPSAETLWKFIARNGRHYLPKL"
     gene            complement(2720..>4635)
                     /locus_tag="VIW27_01750"
     CDS             complement(2720..>4635)
                     /locus_tag="VIW27_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5738413.1"
                     /translation="LHLGSASLAEAIPRAVDEVGGPTILATLTVVAALLPMAFVSGLM
                     GPYMSPIPINASIGMLISLAVAFVFTPWLGLKLLGRKASSQHASEKSDQALYPLFSRI
                     ITPLLAGSRGRRNRWLLLVAIIALILASISLAGFKLVVLKMLPFDNKSEVQVVVDMPE
                     GTSLERSARVIAELSDYLAEVPEVTDYQAYVGSASPINFNGLVRQYYLRDAANLSDIQ
                     INLRHKSLRERDSHEIALALRRPLQEIGERHQANVRIVEVPPGPPVQAPLVVEIYGID
                     YDGQIRVAREVREVLAQSPGIVDIDDSVETESARWILAIDREKATRLGLDQAAIAGTI
                     ATLVDGEDIGYLHRDDIKYPLPLRLQLSDGDRAELGKLLSLKLRAAHGKLVALSEVVE
                     LRQAVNEHSIYHKDLLPVVFVTADMAGEVDSPLYGMFDVMFRLQEPSALASLDAPIEQ
                     YLIRQPDNPYRYSLKWDGEWQVTYETFRDMGLAYSVGLILIYLLVVAQFRSYATPLVI
                     MAPIPLTVVGVLPGHALLDAQFTATSMIGMIALAGIIVRNSILLVDFINQQVAEGVEF
                     GEAVIQAATVRARPIALTAMAAALGAFFILDDPIFNGLAIALIFGILVSTLLTLIVIP
                     VLYYVLKRREYAH"
BASE COUNT          888 a         1430 c         1460 g          857 t
ORIGIN      
        1 gggcacgcgc tggaaattga tatgcgtgcg cccgaactgg ggcacgatgt agttcacgta
       61 atcgggcatg cgccgcagta tggtgtcgac cacggcctcg gtggaatagc cgcgcgtcgc
      121 cttgtcgcgg tgcagcttct ggatccattc caggttgatg atgggcacca cgcccaccag
      181 caggtcggcg tggcgcgcga tgtcgacttt gtcggtgacc accgcgccgt gcaggccctc
      241 gtagaacagc aggtcggtac cctcggcaaa ctcttcccag tcggtgaaag ttcccggcgg
      301 caccccgtag cgctcggact cagcgtcgtc gtgtacatag tgccgggtac gcccggtgcc
      361 ggtttcgcta tactggcgaa acacgtcctc gagttcctcg aacaggttgg cctcgggtcc
      421 gaaatgactg aaatgcttgc tgccgttggc ttccgcgtcc gccatcgcgt gtttcatttc
      481 catccggttg tagcggtgga aggcatcccc ctcgatgtag caggcatcga tgttttcgcg
      541 ggtgaagatt tgctggaagg tttccttgac cgtggtcgtg ccggctcccg aggaaccggt
      601 tatcgacagg atgggatgtt tgcctgacat gctcgacctc aatttctgag caatccgcgt
      661 tgctcgaaca gcgggaagcg ggtggcctga aacgcggtgc ccgagacgta atcgaagacc
      721 tcggctaccc ggtcgcggga gccgaatacc agcggtaccc gccgatgcag cgattcgagg
      781 cgtaaatcga gaatgcgctc gcggccgtcg ctggcactgc cgccggcctg ttcgacgatc
      841 atcgccatcg gcaccgcctc gtacagcaag cgcaggcgac cgtcggcata acccggccgt
      901 gaatcgccgg gatagagaaa tactccgccg cgcatcagaa tgcggtacgc ctcggcaacc
      961 agcgaggcat tccagcgcat gttgaagtca tggcccaggt tgccgctctc gccggcaata
     1021 caatcgtcga tgaagtgcct gacgctgggc tcccagaagc gataattgga aacgttgatc
     1081 gcgaactcgc atttgccgtt gggaatctgc agcggctcgc cgatggtgcg gaattcgccg
     1141 ttggcgggat cgagcagaaa aacctggctg ccctctcccc aggtcagcac cagtcgggtc
     1201 tgtggaccga aacagacgaa accggcggcg cgttgccgtt cacccgggca gatatctgtc
     1261 agttcgtcga cgccggcctc gagcaccgaa aagatggcgc cgatcgaaag gttggtttcg
     1321 atattggaag atccatcgac gggatcaatg gcgaccccgt acctggcacc gcgatcgatg
     1381 acgatcggcg tctccgcttc ctccgaggcg atgaatccga tcggcgagtg gcaaagcgca
     1441 tcgacgtaaa gctggtgagc gaagctatcc agcggctcca ccggatcgcc gtcggtattg
     1501 cgctcgccgg cggaatcgac ggcagccagc tcgtgccgcg cgatcgattt cgaaagctcg
     1561 gcgctggtgc gggtaatcgt cttgaggatt tcggcaagcg cgatgcgtgg cgcatcggcg
     1621 ccggcccagt tttccagtat tgtcgccaga ctgattcccg tagacatgat aggcagaact
     1681 ccgaacacga ataatgcggc cgagtatgcg gtgattgatt atttagtaaa gtttaataaa
     1741 cttacaaata atgaaagaaa aactttaccc ctggttacgt caactaacct tgcggcagat
     1801 gcgcggtttc gaggcagtgg tcgattccgg tagcgtctcc gcggcagccc gcagcctgca
     1861 cctgacgcca ccggcggtat ctctgcagct gcgcgatctc gaaaacgcga tcggtctgcc
     1921 gttgctggaa cgcggtgagg acgggctcgt gaccacccgg gtcggccagg aattactgga
     1981 aatgtcgcgc gaattccagg ccagcctgga tgctttcgcc gagcgtatcg acgaattgaa
     2041 aggcgtcgac cacggcgtgg tgtcggtcgg cgtcgtcagc accgcgcgtt acttcgcacc
     2101 gatggtgctg gcggagtttc tcaagcttta ccccgaggtc cgcatccagc tgcaggtcgg
     2161 caaccgcgat gcgacgctgg ccaaactcga gcgcctggag ctggatttcg cgatcaccgg
     2221 ctcgccgccc gagcatttcg aggtcaagcg cgagtacatc ggcaagaacc cgcagatcct
     2281 gatcgcgccc cccgatcatc cgctggcgaa caaagcccgc attccgctat ccgggcttcg
     2341 cggccagact tttctgttgc gcgagcccgg ctcggggaca cgcgcggtgg ccgaccgcct
     2401 gttcgcgcgc gaccgggaac tacccggcaa cgcgctcgaa ttcggcagca acgagaccat
     2461 caagcaggcg gtcatggcag gactcgggat cgcgctaatt tcggcgcata cggtggccgc
     2521 ggagctcgac gagaagcggc tggtggcgct aaacgtccgc ggcttgccaa ttcatcgcaa
     2581 gtggtttctg gtcaagcatg ccggcaagcg ctttctgccg tcggccgaaa ccctgtggaa
     2641 attcatcgcc cgcaacggcc gccattacct gccgaaactc tagttatgcc gcggcccggg
     2701 aaagcggaga tcgctgcaat cagtgcgcgt attcgcgccg cttgagcacg tagtaaagca
     2761 ccgggatcac gatcagcgtc aacagcgtcg acaccaggat gccgaagatc agcgcgatcg
     2821 ccaggccgtt gaagatcgga tcgtcgagga tgaaaaacgc gcccagcgcc gcggccatcg
     2881 cggtcagcgc gatcggccgg gcgcggaccg tggccgcctg gatcaccgcc tcgccgaact
     2941 cgacgccctc ggcaacctgc tggttgatga agtccaccag caaaatcgag ttgcgcacta
     3001 taatgccggc cagcgcgatc atgccgatca tcgaggtcgc cgtgaactgc gcgtcgagca
     3061 gcgcatggcc gggcagcacg ccgaccaccg tcagcggtat cggcgccatg atcaccagcg
     3121 gtgtcgcgta ggagcggaac tgtgcgacca ccagcaggta gatcagaatc aggcccaccg
     3181 aataggccag ccccatatcg cggaaggttt cataggtgac ctgccactcg ccgtcccatt
     3241 tcaggctgta gcggtaggga ttgtcgggct ggcgaatcag gtattgctcg atcggcgcgt
     3301 cgagcgatgc cagcgccgac ggttcctgca ggcgaaacat cacatcgaac ataccgtaca
     3361 gcggactgtc gacctcgccc gccatgtcgg cggtgacaaa caccaccggc agcaagtcct
     3421 tgtgatagat gctgtgctcg ttgaccgctt ggcgcaactc gacgacttcc gacagcgcga
     3481 ccagcttgcc gtgggcagcg cgcagcttca gcgacaacag cttgccgagt tcggcgcggt
     3541 cgccgtcgga tagctgcagg cgcagcggca gcgggtactt gatgtcgtcc cggtgcaggt
     3601 aaccgatgtc ctcgccgtcg accagggttg cgatggtgcc ggcgatggcc gcctgatcga
     3661 gacccaggcg cgtggctttc tcgcgatcga tggcgagtat ccagcgggcc gattcggtct
     3721 ccacgctgtc atcgatatcg acgatacccg ggctttgcgc gagcacctcg cgcacttcac
     3781 gagccacgcg gatctggccg tcgtaatcga tgccatagat ctcgacgacc agcggcgcct
     3841 gcaccggagg tcccggcgga acctcgacga tcctgacatt ggcctggtgg cgttcgccga
     3901 tttcctgcag cggccgacgc aatgccaacg cgatttcgtg gctgtcgcgc tcgcgcagcg
     3961 acttgtggcg cagattgatc tggatatcgg aaaggttggc ggcgtcgcgc aggtaatact
     4021 gccgcaccag gccgttgaaa ttgatcggcg atgcgctgcc cacgtaagcc tggtagtcgg
     4081 ttacctcggg tacttcggcg agataatcgc tgagttcggc gatcactcgc gcgctgcgtt
     4141 ccaggctggt gccctcgggc atgtcgacga ccacctgcac ctccgatttg ttgtcgaacg
     4201 gcagcatctt caggaccacg agcttgaacc cggcgaggct gatcgaagcc aggatcagcg
     4261 cgatgatcgc caccagcagc agccagcggt tgcgccgccc gcggctgccc gccaacaacg
     4321 gcgtgatgat gcggctgaac agcgggtaca gcgcctggtc cgatttttcg ctggcgtgct
     4381 gcgacgatgc cttgcggccg agcagcttga gaccgagcca cggcgtaaac acgaaggcta
     4441 ccgccagtga tatcagcatg ccgatactcg cgttgatcgg tatcgggctc atgtaggggc
     4501 ccatcaggcc gctgacgaag gccatcggca gcagcgccgc aaccacggtc agtgtcgcca
     4561 ggatggtcgg cccgccgacc tcgtcgacgg cacgcggaat cgcttcggca aggctggcgc
     4621 tgcccaggtg caggt
//
LOCUS       DATCZV010000078         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_481838, whole genome shotgun sequence.
ACCESSION   DATCZV010000078 DATCZV010000000
VERSION     DATCZV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481838"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..460
                     /locus_tag="VIW27_01755"
     CDS             104..460
                     /locus_tag="VIW27_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEY5738414.1"
                     /translation="MSDIQDYQSSLADPASRKFETFSYLPEMTAAQIRRQVEYIVSRG
                     WNPGIEHTEPENASGNYWYMWKLPMFGEADVDTIISECVACHNAHPDNHVRLLGFDNY
                     AQCAATSMVIYRGNPV"
     gene            497..1303
                     /locus_tag="VIW27_01760"
     CDS             497..1303
                     /locus_tag="VIW27_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY5738415.1"
                     /translation="MVDSDREQYLVTEEPFYRAVADEVTNYEAAYRVRMPVMLKGPTG
                     CGKSRFVEYMAWKLQKPLISVACTEDMTASDLVGRHLLDIDGTRWQDGPLTVAARIGA
                     ICYLDEVVEARQDTTVVIHPLTDHRRELPLEKKGEMVHAHEDFQLVISYNPGYQSLMK
                     DLKQSTKQRFGGIDFDYPQAEVEAEIVTHEGGINLDIATKLVQVGASSRNLKGHGLDE
                     GMSTRLLIYAAQLMAQGVDAAAACRMALVTPLTDDPDMRDTLHAAIDTYF"
     gene            1544..>3652
                     /locus_tag="VIW27_01765"
     CDS             1544..>3652
                     /locus_tag="VIW27_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5738416.1"
                     /translation="MSANKMHPELAEYLQCIDPADLGDAGLVEASWLEAQRIMSPQGL
                     ENYLVGIKAMCTLGKGQDLVLTYVQEMPSVVREVGEDIVPDVIEALMKMASLTSGSVI
                     TLILANLPLAATRLGDAELLRGYLKLLHQLAGKAPRGLRPMMACLSELLSKLTLGGLR
                     RWANWGAQAYQRDFDGQSAYFGLATETSKSILQRERRGTLFVDNHRKLNFYLRALWGR
                     AFFMRPTAEDFETREGGKPYIESNLVHLPDAFDRYHDIEGVDVYRAAAAHAAAHIVHT
                     DSGLDGDSLSPAQRCCVEVFEDARVEYLAWRRFPGLRRLWLGFFEHALAQEKDPPHPM
                     VELMLRMSRSLLASDYVDAQPWLAALAAGFRSRLDANAEDASISLEFGLELFDRIREQ
                     TQVPSLRILQQTPIPYRDDNRYPWAFSGDALDAGEYERLPWRQPTLRKYVNLMQMVNE
                     IDSELEDGSPQEIWVLPTELFPYEDEGVSYNQAEGVEPMSDPFHYDEWDYQVQMSRPQ
                     WTTVIERRQARGDAEIMDGILQKHKPVASRIKHLIDALQPQGIVRRRGYEDGDELDID
                     AAVRAMIDIRRGIMPDPRINIRISRHYRDLSMIVLMDLSQSTNDRIGDLGEDDDGYLE
                     QPRVLDLTREATGLLSWAVDAIGDNFAVHGFASDGRHDVRYYRFKDFDEKYVDDAKAR
                     LAGMQGGLSTRMGAALRHAGW"
BASE COUNT          733 a         1107 c         1152 g          660 t
ORIGIN      
        1 tggagacctg gaaggaaatc aagttcgagt tcgataccgt cgacaagctc gacgttgccc
       61 atcggtaacc ggctttcggc acagaattca agaggaatca atcatgagcg atattcaaga
      121 ctatcaatcg agcctcgccg atccggcgag ccgcaagttc gaaacctttt cctacctgcc
      181 ggaaatgacc gcggcacaga tccgccgcca ggtcgagtac atcgtctcca ggggctggaa
      241 cccgggcatc gagcacaccg agcctgaaaa tgcgtcgggc aattactggt atatgtggaa
      301 gctgccgatg ttcggcgaag ccgatgtcga taccatcatc agcgagtgtg tcgcctgcca
      361 caacgcgcac cccgacaacc atgtacgcct gctcggcttt gataactatg cccagtgcgc
      421 cgccacgtcg atggtcattt accgcggtaa ccccgtctag gcggttgtca tggctacagc
      481 ggcaagtcag atgcagatcg tcgatagcga ccgcgagcag tacctggtaa cggaagaacc
      541 gttttaccgg gcggtcgccg acgaggtgac gaactacgag gcggcttacc gggtgcgtat
      601 gccggtgatg ctgaaaggcc ccaccggctg cggcaagtcg cgcttcgtcg agtacatggc
      661 ctggaaactg cagaagccgt tgatcagtgt cgcctgtacc gaggacatga ccgcgtccga
      721 cctggtcggc cgacacctgc tcgatatcga cggcacgcgc tggcaggacg gcccgctgac
      781 cgtcgccgcg cgtatcggcg cgatctgtta cctcgacgag gtggtcgagg cgcgccagga
      841 taccacggta gtgattcatc cgctgaccga ccaccggcgc gaattgccgc tcgagaaaaa
      901 gggcgagatg gtgcatgcgc acgaggattt ccagctggtg atctcgtaca acccgggcta
      961 ccagtcgctg atgaaagacc tcaagcaatc gaccaagcag cgcttcggcg gtatcgattt
     1021 cgactatccg caggccgagg tcgaggccga gatcgtcacc cacgagggcg ggatcaatct
     1081 cgacattgcg acgaagctgg tccaggtggg cgccagttcg cgcaacctga agggtcacgg
     1141 cctggacgag ggtatgtcga cgcgactgct gatttacgcc gcgcagctga tggctcaggg
     1201 tgtcgatgcc gcggcggcct gccgtatggc gctggtgacg ccgctgaccg acgatccgga
     1261 tatgcgcgat acgctgcacg ccgccatcga tacctatttc tgatggagat cacgagtcac
     1321 ccccgcgcaa gcggagacct tccggggtgg gggcgtcacc ctcgcgcaag cggggatctt
     1381 ccagaggggg gtccgcgtca tccccgcaca agcggggatc ttccaggggg tcgccgttac
     1441 cttcgcgcaa gcggggatct tccagagggg ggtaccggtc atccccgcgc aagcggggat
     1501 cttgaatcgt cgccggaaat gccagattcg cgctcgtgcg cgaatgagcg caaataaaat
     1561 gcaccccgaa ctcgccgagt atctgcaatg catcgatccc gcggacctgg gcgatgcggg
     1621 cctggtcgag gcgtcctggc tcgaggcgca gcgcatcatg tcgccgcagg gattggagaa
     1681 ttacctggtc ggcatcaagg caatgtgcac cctcggcaag ggccaggacc tggtattgac
     1741 ctatgttcag gaaatgccgt cggtggtcag ggaagtcggc gaggatatcg ttccggacgt
     1801 catcgaagcg ctgatgaaga tggcgtcgct gacctcgggc tcggtgatca cgctgatcct
     1861 cgccaacctg ccgttggcgg caacccgtct cggcgatgcc gaattgctgc gcggctacct
     1921 caagttgctg catcaactgg ccggcaaggc gccgcgcggc ctgcgcccga tgatggcatg
     1981 tttgagcgaa ctgttgtcca agctaacgct gggcggcctg cgtcgctggg ccaactgggg
     2041 agcacaggct taccagcgcg atttcgacgg gcaaagcgct tacttcgggc tcgccaccga
     2101 aacctcgaaa tcgattttgc agcgcgaacg ccgcggcacg ctgttcgtcg ataaccaccg
     2161 caagcttaat ttttacctgc gcgcgctgtg gggcagggct ttcttcatgc gcccgaccgc
     2221 cgaggacttc gaaacccgtg agggcggcaa accctacatc gaatctaacc tcgtgcattt
     2281 accggacgcg ttcgatcgct accacgatat cgaaggcgtc gacgtttacc gcgccgcggc
     2341 ggcgcacgcc gcggcgcata tcgtgcatac cgacagcggg ctcgatggtg acagcctgag
     2401 cccggcgcag cgttgctgcg tcgaggtatt cgaagacgcg cgggtcgaat acctcgcctg
     2461 gcggcgcttt ccgggtttac gccggctgtg gctgggtttt ttcgagcatg cgctggcgca
     2521 ggaaaaagac ccgccgcacc cgatggtcga actgatgttg cgcatgagcc gcagcctgct
     2581 cgcttcggac tacgtcgacg cgcaaccctg gttagccgcc ctcgcggccg ggtttcgcag
     2641 ccggctggac gccaacgccg aagacgcttc gatcagcctc gagttcgggt tggaattatt
     2701 cgaccgtatt cgcgagcaga cgcaagtgcc gtcgctgcgc attctccaac agacgccgat
     2761 accctaccga gacgataatc gatacccctg ggcgttcagt ggcgacgcgc tcgatgccgg
     2821 cgagtacgag cgactgccct ggcgtcagcc gacgctgcgc aaatatgtca acctgatgca
     2881 gatggtcaac gaaatcgatt ccgaactcga ggacggcagc ccgcaggaaa tctgggtatt
     2941 gccaaccgaa ctgtttccct acgaggacga gggcgtcagc tacaaccagg ccgagggcgt
     3001 cgagccgatg tccgatccgt tccattacga cgaatgggac taccaggtgc agatgtcgcg
     3061 gccgcaatgg actaccgtga tcgagcgccg gcaggcgcgc ggcgatgccg aaatcatgga
     3121 cggcattttg caaaaacaca aaccggtcgc tagccggatc aagcacctga tcgacgcatt
     3181 gcagccacag ggaatagtgc gccggcgagg ttacgaggat ggcgacgaac tcgatatcga
     3241 tgccgcggtg cgggcgatga tcgatatccg ccgcggcatc atgcccgatc cgcggatcaa
     3301 tatccgtatc agtcggcact accgcgatct gtcgatgatc gtgctgatgg acttgtcgca
     3361 atcgaccaac gaccgtatcg gtgatctcgg cgaggacgac gacggctacc tcgaacagcc
     3421 gcgggtcctc gacctgaccc gcgaggcgac cgggctattg tcgtgggcgg tggacgcgat
     3481 cggcgataac ttcgcggtac acggcttcgc ctcggacggc cgtcacgacg tgcgctatta
     3541 ccgtttcaag gatttcgacg agaaatacgt cgacgatgcc aaggcccgcc tcgcaggaat
     3601 gcagggcggt ctgtcgaccc gcatgggagc cgcgctgcgg catgcgggct gg
//
LOCUS       DATCZV010000079         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_588168, whole genome shotgun sequence.
ACCESSION   DATCZV010000079 DATCZV010000000
VERSION     DATCZV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588168"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="infC"
                     /locus_tag="VIW27_01770"
     CDS             <1..418
                     /gene="infC"
                     /locus_tag="VIW27_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5738417.1"
                     /translation="EAQALADEAGVDLVELVPDAAPPVCRLMDYGKFKFQQKKKLHDQ
                     RKKQRQVHIKEIKFRPGTEEGDYQVKLRKLTEFIEVGDRVKVTVRFRGREMAHMELGT
                     QLLKRVEDDTREFATVEQYPQSEGRQMTMLLVPKKR"
     gene            503..700
                     /gene="rpmI"
                     /locus_tag="VIW27_01775"
     CDS             503..700
                     /gene="rpmI"
                     /locus_tag="VIW27_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY5738418.1"
                     /translation="MPKMKSVSGAAKRFKRSPSGGYKRKQSHLRHILTKKSSKRKRQL
                     GEKLEVHENDVLQVRRMLPYS"
     gene            713..1075
                     /gene="rplT"
                     /locus_tag="VIW27_01780"
     CDS             713..1075
                     /gene="rplT"
                     /locus_tag="VIW27_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5738419.1"
                     /translation="MPRVKRGVTAHARHKKVLEKAKGYRGARSKVFRVAKQAVTKAGQ
                     YAYRDRRQRKRQFRALWIARINAGARENGMSYSVFMNGLKKAAVEIDRKVLSDIAVHD
                     KPAFTALVEKARAGLETA"
     gene            1198..2157
                     /gene="pheS"
                     /locus_tag="VIW27_01785"
     CDS             1198..2157
                     /gene="pheS"
                     /locus_tag="VIW27_01785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY5738420.1"
                     /translation="MAALDAVRVAYLGKKGEITAQLKNLGQLPADERPAAGQAINRVK
                     QEIQQLLETRRAQLGDEAIAERLQRETVDITLPGRRVAAGGLHPVTLTMNRIGLMFGK
                     LGFDVAEGPEVEDDFHNFEALNIPEHHPARAMHDTFYFDDGMLLRTHTSPVQIRVMEN
                     QPPPLRVIAPGRVYRCDSDLTHTPMFHQVEGLLVDEEVSFSVLKGTLEEFLKMFFEQE
                     DLQTRFRPSYFPFTEPSMEVDISCVICAGEGCRVCSQTGWLEVLGCGMVHPELYRHTG
                     VDAEKYTGYAFGMGVERLAMLRYGVNDLRLFFENDLRFLRQFN"
     gene            2167..>2658
                     /locus_tag="VIW27_01790"
     CDS             2167..>2658
                     /locus_tag="VIW27_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY5738421.1"
                     /translation="MEISENWLREWVDPPLDSERLASQLTMAGLEVGGSRAAAPALDG
                     VVVAEVLAVEPHPDADKLAVCRVGDGDAEFTVVCGAPNVRAGLKVALARVGTHLPGIE
                     IKQSKIRGVVSEGMLCSAAELELAEASGGILELPAQAPPGVPLVDYLELADTIIDIDL
                     TPNR"
BASE COUNT          551 a          803 c          854 g          450 t
ORIGIN      
        1 agaggcgcag gccctggcgg atgaagccgg ggtggatcta gtcgaactgg tacccgatgc
       61 cgcgcctccc gtgtgccggc tgatggatta cggcaagttc aagtttcagc aaaagaagaa
      121 gttgcacgac cagcgcaaga aacagcgcca ggtgcatatc aaggaaatca agtttcgacc
      181 cggcaccgaa gagggtgatt accaggtcaa gctgagaaaa ctgaccgagt tcatcgaggt
      241 cggtgaccgc gtcaaggtga cggtgcgctt tcgcggccgc gaaatggcgc acatggaact
      301 gggcacgcag ttgctcaagc gggtagagga tgatacccgg gaattcgcga ccgtggagca
      361 gtatccacag tcggaagggc gccagatgac gatgttgctg gtgccgaaaa aacgctagcc
      421 gaaaaggccg gcgataaccc gaagacgcgt ctccggcgtc cagggccatt ttagttatta
      481 gacatttaaa ctggagtaaa tgatgccaaa gatgaaatcc gtcagtggcg ctgccaagcg
      541 tttcaagcgc tcgccttcgg gcggctacaa gcgcaagcaa tcgcacctgc gccatatact
      601 gaccaagaaa tcgagcaagc gtaagcgcca gctcggcgag aagctcgaag tccatgaaaa
      661 cgacgtgctg caagtccgcc gcatgttgcc gtattcctag gagggtagag cgatgccaag
      721 agtcaagcga ggcgttaccg cacatgcccg ccacaagaag gtgctggaaa aggccaaggg
      781 ataccgcggc gcgcgcagca aggtttttcg agtcgccaag caggcggtca ccaaggccgg
      841 ccagtacgcg taccgtgacc gtcggcagcg caagcgccag ttccgcgcgc tgtggatcgc
      901 gcgcatcaat gccggcgcgc gtgaaaacgg catgtcctac agcgtgttca tgaacggtct
      961 gaagaaagcc gcggtcgaaa tcgatcgcaa ggtgctttcc gatatcgcgg tgcacgacaa
     1021 gcccgcgttc acggcgctgg tcgaaaaggc cagggccggc ctggagactg cgtagcagtc
     1081 gattcggggc ggcggcaaat tctgctttgc cgccgccgac ataaatctcg atacgtgacc
     1141 caggatctcg acactctgct ggagcaagcc tcccgttcga tcagccagtc cagcgacatg
     1201 gccgcgctcg atgccgtgcg cgtcgcttat ctcggcaaga agggcgagat taccgcgcaa
     1261 ctgaagaacc tgggacagtt gccggccgac gagcggccgg cggccggtca ggcgatcaat
     1321 cgcgtcaagc aggaaatcca gcaattgctg gaaacccgcc gtgcgcagtt gggtgacgaa
     1381 gcgatcgccg agcgcctgca gcgcgaaacc gtcgatatca ccctgccggg caggcgcgtc
     1441 gccgccggcg gcctgcaccc ggtgacgctg accatgaacc gcatcggcct gatgttcggc
     1501 aagctcggtt tcgacgtcgc cgaggggccg gaagtcgagg acgacttcca caatttcgag
     1561 gccctcaata ttcccgagca ccacccggca cgcgcgatgc acgatacctt ctatttcgac
     1621 gacggcatgc tgttgcgcac ccatacctcg ccggtgcaga ttcgagtgat ggaaaaccag
     1681 ccgccaccgc tgcgcgtgat cgcgccggga cgcgtctacc gctgcgactc ggacctgacg
     1741 catacgccga tgttccacca ggtcgaaggc ttgctggtcg acgaagaggt ctcgttctcg
     1801 gtgttgaagg gtacgctcga ggaattcctc aagatgtttt tcgagcagga agacctgcaa
     1861 acccgttttc ggccgtctta cttcccgttc accgagccgt cgatggaagt cgatatcagt
     1921 tgcgtgatct gcgccggtga gggttgccga gtgtgcagcc agaccggctg gctcgaagtg
     1981 ctgggctgcg gcatggtgca tcccgagctg tatcgccaca ccggcgtcga tgccgagaag
     2041 tataccggct acgcgttcgg catgggtgtc gagcggctgg cgatgctgcg ctacggcgtc
     2101 aacgacctgc ggctgttctt cgagaacgac ctgcgtttcc tgcggcagtt caactagggc
     2161 gcggcgatgg aaatcagcga aaactggctg cgcgagtggg tcgatccgcc gctcgacagc
     2221 gaacggctgg cgagccagct gaccatggcc gggctcgagg tcggcggctc gcgcgccgct
     2281 gccccggcgc tcgacggagt ggtggttgcg gaagtgctgg cggtcgagcc acaccccgat
     2341 gccgacaagc tcgccgtttg ccgggtcggc gacggcgacg ccgaattcac ggtcgtctgc
     2401 ggcgccccca acgtgcgtgc cggcctcaag gtggcgctgg cgcgcgtcgg cacgcacctg
     2461 ccgggtatcg agatcaagca gagcaagatc aggggcgtcg tctccgaggg catgctgtgc
     2521 tcggccgccg aactcgaact cgccgaggct tccggcggca tcctcgaact gcccgcacaa
     2581 gcaccgccgg gcgtgccgct cgtcgactat ctcgaactcg ccgacacgat catcgatatc
     2641 gacctgaccc cgaaccgc
//
LOCUS       DATCZV010000080         4029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_5878, whole genome shotgun sequence.
ACCESSION   DATCZV010000080 DATCZV010000000
VERSION     DATCZV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5878"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VIW27_01795"
     CDS             complement(<1..553)
                     /locus_tag="VIW27_01795"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY5738422.1"
                     /translation="MMHQTKDLRITGTKEIQSPGALMQELPLSEAAANLVTDTRQQIY
                     DVLDGKDDRLVVVIGPCSIHDSRAALEYADRLQKMIDELRDELVIVMRVYFEKPRTTV
                     GWKGLINDPDLDGSFHINKGVRKARELLINLAEKGVPAGHEYLDLISPQYISDLVCWG
                     AIGARTTESQGHRELASGLSCPVG"
     gene            complement(620..1621)
                     /locus_tag="VIW27_01800"
     CDS             complement(620..1621)
                     /locus_tag="VIW27_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY5738423.1"
                     /translation="MKQFDIYALGNALLDIEYHSTPEALADLGIDKGVMTLIDEDRQH
                     GLMARLGESHEKMTCGGSAANTVIAMAQLGASAHFDCRVSNDESGQIYVRDLHDSGVN
                     SILDSKPLPDGVTGKCLVFVTPDTDRTMNTFLGVSAELDVDDIDAEAIKGSQMIYIEG
                     YLASAANTRDAAIEARRIAEAHGNRTSITLSDPNMVRLFRDSLEAMIGDRVDLLFANE
                     DEAMELAATDSLESAVELFKQRARSFAITRGKHGSLLYDGESLIEVKPTVVDAVDSLG
                     AGDMFAGAFLYGLSEGMSYAQAGELASRAAARLVTEFGPRLEPAETRAVLHAFKSGN"
     gene            1888..2307
                     /locus_tag="VIW27_01805"
     CDS             1888..2307
                     /locus_tag="VIW27_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY5738424.1"
                     /translation="MSLKGSQTEQNLKDAFAGESQANRRYLYFAAKADVEGFNDVSAV
                     FRSTAEGETGHAHGHLEYLEEVGDPATGLPIGSTENNLKASIAGETHEYTDMYPGMAK
                     TARDEGFDEIADWFETLAKAERSHANRFQKALDNLDA"
     gene            2368..3705
                     /locus_tag="VIW27_01810"
     CDS             2368..3705
                     /locus_tag="VIW27_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY5738425.1"
                     /translation="MSTSSREGSLEAPTRHPLGQNDPDFYDQQSLFEELERVFDICHG
                     CRRCVSLCNAFPTLFDLVDESPTMEVDGVDKKDYWKVVDHCYLCDLCYLTKCPYVPPH
                     EWNVDFPHLMLRAKANGFKQGRARLRDRIVSSTDKVGSFASIPIVANVVNAANRNEHT
                     RALLESSLGIHADARLPEYHSNRLSKRSRNHRPGEHEVRRTARTTGKVAIFATCYGEY
                     NEPQIGEDLLAVFEHNQIPTMVVRDTVCCGMPKLELGDLDAVGKLKDHNIPLLTKLID
                     DGWDIISPVPSCTLQFKQELPLMYPDDEAVARVQQAFFDPFEYLSLRHREGLLNTDFK
                     NSIGKISYHVACHQRVQRIGMKTRDILALIPDTEVDAIERCSGHDGTYAIKQEFHDVS
                     MKIVRPVANRVSKAQPDAYTSDCPMAGKHIAAGLADGSDTQHPISLLRQAYGI"
     gene            3721..>4029
                     /locus_tag="VIW27_01815"
     CDS             3721..>4029
                     /locus_tag="VIW27_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY5738426.1"
                     /translation="MIQPITRDDLMSLEQYAEKRGEFRQQVLEHKKHRQIALGPNATL
                     YFEDRLTLLYQIQEMLRIEKVFEADGINEELEAYNPLIPDGRNFKATFMIEYPDPQVR
                     A"
BASE COUNT          830 a         1289 c         1126 g          784 t
ORIGIN      
        1 agccgaccgg gcaggacagg ccagaggcca gctcgcggtg cccctggctt tcggtggtgc
       61 gcgcaccgat cgcgccccag cagaccaggt cggaaatgta ctgcgggctg atcaggtcga
      121 gatactcgtg tcctgccggc acgccttttt ccgccaggtt gatcaacagt tcacgcgcct
      181 tgcgtactcc tttgttaatg tgaaagctgc cgtcgagatc cggatcgttg atcaatcctt
      241 tccagccgac cgtggtgcgc ggtttctcga agtatacgcg catcacgatc accagttcgt
      301 cgcgcagttc gtcgatcatc ttttgcagac gatccgcgta ctcgagcgcc gccctggaat
      361 cgtgtatcga gcagggtccg atgaccacca ccagccggtc gtccttgccg tcgagcacgt
      421 cgtagatctg ctggcgggtg tcggtgacca ggttggcggc cgcttcgctc aatggcaatt
      481 cctgcatcag ggctcccggc gactgtattt ccttggtgcc ggtaattcgc aggtccttgg
      541 tttggtgcat gatcatcagg ttggcttccg cgcgctgcta tgtcgttgaa aaagggcgat
      601 attctcaacc atcgtcgggc taattgccag acttaaatgc gtgcagcacc gcgcgcgtct
      661 cggcgggctc gagccgcggg ccgaattcgg tgaccaaccg cgccgcggct ctcgaggcga
      721 gctctccggc ctgcgcgtaa gacatgccct cgctcagccc gtagaggaag gcgccggcga
      781 acatatcacc cgcccccagc gaatcgacgg cgtcgaccac cgtcggtttt acctcgatca
      841 gcgactcgcc gtcgtagagc agcgagccgt gcttgccgcg cgtgatcgca aagctgcgcg
      901 cacgctgctt gaaaagctca accgccgatt cgagtgaatc cgtcgccgcc agttccatcg
      961 cctcgtcctc gttggcgaac agcaggtcga cgcggtcgcc gatcatcgcc tccagcgaat
     1021 cgcgaaacaa gcgcaccatg ttcggatcag acagcgtgat cgaagtcctg tttccgtgcg
     1081 cctcggcgat ccgccgcgcc tcgattgccg catcccgggt attcgcggcg ctcgccagat
     1141 atccctcgat gtagatcatc tgcgaaccct tgatcgcctc ggcatcgatg tcgtcgacgt
     1201 cgagctccgc gctgacgccg agaaaggtgt tcatcgtgcg gtcggtgtcc ggagtgacga
     1261 agaccaggca tttgccggtc acgccgtccg gcagcggctt gctgtcgagg atcgaattca
     1321 cccccgagtc gtgcaggtcg cgcacgtaga tttgtcccga ctcgtcattc gatacccggc
     1381 aatcgaagtg cgcgctggca ccgagctgcg ccatcgcgat caccgtgttg gcggccgatc
     1441 cgccacaggt cattttttca tgggattcgc ccaaccgtgc catcaggcca tgctggcggt
     1501 cctcgtcgat gagcgtcatg acgcccttgt cgatgcccag gtcggcgagc gcctcggggg
     1561 tcgaatggta ctcgatgtcg agcagcgcgt taccgagcgc gtaaatgtcg aattgtttca
     1621 taacccttct tctatctgtg tatgagcgga atcaacagcc tgccgttccg cctgtcgcat
     1681 cgttgtcacg gaagccgctg cgggacttca ccgagcggtc tcgcgaagac cggaagcgac
     1741 ctcgggcacc gctttcgggc gctcggcgag gcaggtcact ttattgaaaa catatattaa
     1801 atatataaat aaaattaact tcatttatat attcgggctg ttaatatacc gatcccgaga
     1861 ccttaattcc acacatctgg aggcatcatg tcccttaaag gatcacaaac cgaacagaat
     1921 ctgaaagacg ctttcgccgg tgaatcgcag gccaatcgcc gctacctgta ctttgcagcc
     1981 aaggctgacg tcgaaggctt caatgacgta tccgcggtat ttcgttcgac cgccgagggt
     2041 gagaccggcc acgcgcacgg tcatctcgag tacctcgagg aagtcggcga tcccgctacc
     2101 ggcctgccga tcggcagcac cgagaacaac ctgaaggctt ccatcgccgg tgaaacccac
     2161 gagtacaccg acatgtaccc gggcatggcg aagactgccc gcgacgaagg ttttgatgaa
     2221 atcgcggact ggttcgagac cctggcgaag gccgaacgtt cccacgccaa ccgtttccag
     2281 aaggcgctgg acaacctcga cgcttaatct atctttactc gcccgggtcc ggcactgcgg
     2341 acccgggcat atattcaggc caaacctatg tctacttcat cccgagaagg cagcctggaa
     2401 gcgcctacgc gacatcccct ggggcagaac gacccggact tctacgacca gcaatccctg
     2461 ttcgaagaac tggagcgggt gttcgacatc tgccatggct gccgccgctg cgtcagtctc
     2521 tgcaacgcgt ttccgaccct gttcgacctg gtcgacgaat ccccgaccat ggaagtcgac
     2581 ggcgtcgaca agaaagatta ctggaaggtc gtcgaccact gctacctgtg cgacctgtgt
     2641 tacctgacca agtgtccgta cgtaccgccg cacgagtgga atgtcgattt cccgcacctg
     2701 atgctgcgcg ccaaggcgaa cggtttcaag cagggccgcg cgcgcctgcg cgaccgcatt
     2761 gtttcatcga ctgacaaggt cggcagcttt gccagcattc cgatcgtggc caatgtcgtc
     2821 aacgcagcca accgtaacga acacacccgc gcactgctcg aatcctcgct cggcatccat
     2881 gccgatgcac gcttgccgga gtatcacagc aatcgcctca gcaagcgcag ccgcaaccac
     2941 cggcccggcg agcacgaggt caggcgcacc gcgcgtacca ccggcaaggt cgcgattttc
     3001 gccacctgct acggtgagta caacgagccg cagatcggcg aagacctgct cgcggtcttc
     3061 gagcacaacc agattccgac gatggtcgtc cgtgacaccg tctgctgcgg catgccgaag
     3121 ctcgaactcg gcgacctgga cgcggtcggc aaactcaagg accataacat tccgctgctg
     3181 acaaaactga tcgacgacgg ctgggatatt atttcgccgg ttccttcctg cacgctgcag
     3241 ttcaagcagg agctgccgct gatgtacccc gacgatgaag ccgtcgccag agtccagcaa
     3301 gccttcttcg accccttcga gtacctgtcg ctgcgccacc gcgagggact gctgaacacc
     3361 gatttcaaaa attcgatcgg caagatcagt tatcacgtcg cctgccacca gcgcgtgcaa
     3421 cgcatcggta tgaagacccg cgacattctg gcgctgattc ccgacaccga agtcgacgcc
     3481 atcgagcgct gctcgggaca cgacggtacc tacgcgatca agcaggaatt ccacgacgta
     3541 tcgatgaaga tcgtcaggcc agtcgccaac cgcgtcagca aggcgcagcc cgacgcctat
     3601 accagtgatt gcccgatggc cggcaagcat atcgcggccg gacttgccga tggcagcgac
     3661 acccaacacc caatcagcct gctgcggcag gcctatggta tctaatacat ggagacccgc
     3721 atgattcaac cgattacccg cgacgacctg atgagcctcg aacaatatgc cgagaagcgc
     3781 ggtgaattcc gccagcaggt gctcgagcac aagaagcacc ggcaaatcgc gctcggcccc
     3841 aatgcgacgc tgtatttcga agaccggctg accctgctct accagatcca ggaaatgctg
     3901 cgcatcgaga aggttttcga ggccgatggc atcaacgagg aactcgaagc ctacaacccg
     3961 ctgattcccg acggacgcaa tttcaaggcc actttcatga tcgagtaccc ggacccgcag
     4021 gtacgcgcc
//
LOCUS       DATCZV010000081         5028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_5931, whole genome shotgun sequence.
ACCESSION   DATCZV010000081 DATCZV010000000
VERSION     DATCZV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5931"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VIW27_01820"
     CDS             <1..310
                     /locus_tag="VIW27_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HEY5738427.1"
                     /translation="DPVVYHRDYRAAFVELLEQVFAQIDAARLHSVSLGSFRLTRDHF
                     RNTARLYPDEPLFAQPLVLDKGIVGYAPEREREMIEFCETELLRHIPAASYHPCEWHG
                     "
     gene            303..1016
                     /locus_tag="VIW27_01825"
     CDS             303..1016
                     /locus_tag="VIW27_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5738428.1"
                     /translation="MAEDNSALVTGASSGIGLETARLLLEQGYRVCGISRRPAPLEHP
                     NFSMLRVDLEDLDALGDALQKLLAKHRFDCLVHAAGRGLFGSIEQFSIAQIDASLRLN
                     LASAMVVCRALLPGLRGRGCGRLVFIGSESALAAGRKGALYSAAKFGLRGFCQALRED
                     CASDGIRVSLVNPGMVRSPFFDQLAFAPGSRPENAIDTGDVAAIVSQILQANPDIVID
                     EINLSPRVKSIDFGGGSRK"
     gene            complement(1033..3768)
                     /locus_tag="VIW27_01830"
     CDS             complement(1033..3768)
                     /locus_tag="VIW27_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5738429.1"
                     /translation="MLSRIYSFKFIIGFALLWLGALAYGLAIFSTYVYRGHAIEAQVE
                     ALQEMLEHESDEAIDGLFDRQKEFAFKLQSEAPFREALARQDPAQMEAWLTESYTRYQ
                     VASGMFRLKTIVVRNLSGEIFAQSSDDGLHGYSGCPTVLSSIGGSLIRLLKPEYALCS
                     SEGQLFAEVLVPVGALEPKAYLHIVAYAVDGLQRIEKLAELPLLIESASGVVLYRSAQ
                     WIGDGVETHLYPSYRLFGDDSFHGVTITAAFEQRRVLERLEPTENSFFVITTLATVSA
                     LLVVLLLLNRAFQPMNKLRNSVGALLTGKYASISEDRLPSELKDLVVAYNEMVEGLEK
                     ETISRRQIEEKLRSEKDFIATTLDSINNPVIVIDSKDRIKLLNPSAEKLFGLRMQELN
                     ETSIHDLLVLYANRQTTRIVDLDQLLNRKLSISSMFFFDASQNLVELEFSASPMIDME
                     AEDVGHVIILKDVSEDRKLRRKLSYEGSHDQLTGFLNRSAFEQKFETLVTEDNDSTPH
                     HVIAYLDIDQFRIVNETCGSAAGDLLLKQLSTMIRKHVRKSDFVSRLSGDEFGIIMPF
                     FEMERALQVIQKIIIEIQHSGFVWNDQEYLVTASIGVMAFGRMADEYADCYSKVTTAC
                     YLAKQNGGNQYHFIDENDEKVMAQQVSMEWVSGIMEGFTEDRFRLYVQPIVSIDEEEK
                     HTHYEVLIRYRRPDGKIVSPSDFLPPAERYNLIERIDGWVVNRIISWLQEHQHLVSDV
                     MFSVNLSGRSIGSQAFHKFLHERLTSSDIDLRQLCFEITETAVVENVEKSVDFINSMK
                     RLGVKFSLDDFGTGLSSFSYLKQFPVDFLKIDGEFVRDIIEDDKSYVFVRSMTEVGHA
                     LEMKVIAEFVESDTMFNKLREANVDFIQGYQVGKPVDIQTLVEQRLTSVNYAGNA"
     gene            complement(3773..4237)
                     /locus_tag="VIW27_01835"
     CDS             complement(3773..4237)
                     /locus_tag="VIW27_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5738430.1"
                     /translation="MTGIIPGRLPLARPAALYGAALIFCAEALATPIAERPESAGQTA
                     VVVTIDAEHLIELYQYVSRLLIIDSRHVEDYVLGHIETSVNLPLASTNCKSLSILANS
                     MERAIVFYSNNSTGDTSIEAIRIASECGYKRLFWLRDGFVEWKSKDYPYVIE"
     gene            complement(4330..4953)
                     /gene="msrA"
                     /locus_tag="VIW27_01840"
     CDS             complement(4330..4953)
                     /gene="msrA"
                     /locus_tag="VIW27_01840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5738431.1"
                     /translation="MFGRPSVIPSAAEALPGRERAMEFDPLHAVLGTSMVAPFPEDCE
                     QIVLGLGCFWGAERKFWSLRGIHTTAVGYAAGHTPNPTYQEVCTGMTGHNEVVLVVFR
                     PREIDFDTLLKIFWESHDPTQGMRQGNDQGTQYRSGIYYLDENQRKAAERSREYYQQK
                     LQASGYGSITTEILPAGPFYYAEDYHQQYLEKNPAGYCGLGGTGVTC"
BASE COUNT         1011 a         1487 c         1451 g         1079 t
ORIGIN      
        1 cgatccggtg gtctatcatc gcgattaccg cgccgcgttc gtcgagctgc tggagcaggt
       61 attcgcacag atcgacgccg cgcggctgca ctcggtcagc ctcggcagtt ttcgcctgac
      121 ccgcgaccat tttcgcaata ccgcgcggct ctacccggac gaacccctgt tcgcgcagcc
      181 gctggtgctc gacaagggta tcgtcggtta cgcgcccgag cgcgaacgcg aaatgatcga
      241 gttctgcgaa accgagctgc tacggcatat tccggccgcc agctaccacc cctgtgaatg
      301 gcatggctga agacaactca gcgctggtca ccggcgccag ttcgggtatc ggcctcgaga
      361 ccgcgcgcct gctgctcgaa caggggtacc gcgtgtgcgg catcagccgg cgcccagcgc
      421 cgctcgaaca cccgaatttc agcatgctgc gcgtcgatct cgaggatctc gacgctctcg
      481 gcgacgcgct gcaaaagctg ctcgccaaac atcggttcga ttgcctggtg catgccgcgg
      541 gccgcggcct gttcggttcc atcgagcaat tctcgattgc gcaaatcgac gccagcctgc
      601 gcctcaacct ggcgagcgcg atggtcgtct gccgtgccct gctgcccggc ctgcgcggcc
      661 gtggctgcgg tcggctggta ttcatcggtt cggaatcggc gcttgcggcc ggccgcaagg
      721 gtgcgcttta cagcgccgcc aagttcggac tcaggggatt ttgccaggcc ctgcgcgagg
      781 attgtgccag cgacggtata cgcgtcagcc tggtcaatcc gggcatggta cgcagcccgt
      841 tcttcgatca actggcattc gctccagggt cgcgacccga gaatgcgatc gacaccggcg
      901 acgtcgccgc catcgtctct cagatactgc aggcaaaccc cgatatcgtt atcgacgaga
      961 tcaacctcag cccgcgggta aaatcaatcg actttggtgg cggtagtcga aagtgacgac
     1021 ggcgcgccag actcaggcgt tgcccgcgta attaacactc gtcagacgct gctcgaccag
     1081 tgtctggata tccaccggct taccgacctg gtacccctgt atgaagtcga cattggcctc
     1141 gcgcagcttg ttgaacatag tgtcggactc gacgaactcg gcgatcacct tcatctccag
     1201 cgcatggccg acctcggtca tcgagcgtac gaaaacgtaa cttttgtcgt cctcgatgat
     1261 gtcgcgcacg aactcgccat cgatcttcag aaagtcgacc ggaaactgct tcaggtagct
     1321 gaaggatgac aggccggtgc cgaaatcatc gagcgaaaac ttgacgccca gccgtttcat
     1381 cgagttgatg aaatccaccg atttctcgac attctcgacc actgcggtct cggtgatctc
     1441 gaagcacagc tgcctgaggt cgatgtcact gctggtcagc cgctcatgca ggaacttgtg
     1501 aaaggcctgc gaaccgatgc tgcgtcccga caggttcacc gagaacatca cgtcgctgac
     1561 caggtgctgg tgttcctgca gccaggaaat gatgcgattg acgacccagc catcgatgcg
     1621 ctcgatcagg ttatagcgct cggccggtgg cagaaaatca ctcggcgaaa cgattttgcc
     1681 atcgggcctg cgataacgaa tcagcacttc gtagtgcgtg tgtttttctt cctcgtcgat
     1741 ggagacaatg ggttgcacgt aaagccggaa tcgatcctcg gtaaagcctt ccatgattcc
     1801 cgacacccat tccatcgaca cctgctgggc catcaccttt tcgtcgttct cgtcgatgaa
     1861 gtgatactgg ttaccgccgt tctgcttggc gagatagcag gcggtggtga ccttcgagta
     1921 gcaatcggca tattcgtccg ccatgcggcc gaaggccatc acgccgatac tcgcggtgac
     1981 caggtattcc tggtcgttcc agacgaaacc cgagtgctgg atttcgatga tgatcttctg
     2041 gatcacctgc agtgctcgct ccatttcgaa gaacggcatg atgatgccga attcgtcgcc
     2101 ggacagccgc gatacgaaat ccgatttacg cacgtgtttc ctgatcatcg tgctgagctg
     2161 cttgagcagc aggtcacccg ccgcgctgcc gcaggtttca ttgacgatgc gaaactggtc
     2221 gatgtcgaga tacgcgatta cgtgatgcgg cgtcgagtcg ttgtcctcgg taaccagggt
     2281 ttcgaacttt tgctcgaagg cactgcgatt cagaaatccg gtgagctggt catgactgcc
     2341 ctcgtaactc agcttgcggc gcagcttgcg gtcctcggag acgtccttga ggatgatgac
     2401 gtggccgaca tcctcggctt ccatgtcgat catcggcgag gccgaaaact cgagctcgac
     2461 gaggttctgg ctggcgtcga aaaagaacat cgagctgatc gacagcttgc gattcagcag
     2521 ttggtcgaga tcgacgatac gcgtggtctg acgattcgcg tacagcacca gcaggtcgtg
     2581 aatcgaggtt tcgttcaatt cctgcattcg caggccaaac agtttttcgg cgctcgggtt
     2641 aagcagcttg atgcgatcct tcgaatcgat aacgatcacc gggttgttga tcgaatccag
     2701 ggtggtagcg atgaaatcct tttccgagcg cagtttttcc tcgatctggc ggcgggaaat
     2761 ggtttccttt tcgagaccct cgaccatctc gttgtaagcg accaccaggt ccttgagttc
     2821 gctcggcagc cggtcctcgc tgatcgacgc atacttgccg gtcagcaacg caccaaccga
     2881 gttgcgcagc ttgttcatcg gctggaaggc acgattcagc agcaatagca ccaccagcag
     2941 ggcgctgacc gtggccagcg tggtgatgac gaaaaaactg ttctcggtag gctcgaggcg
     3001 ttcgaggacc cgacgctgct caaaggccgc ggtgatcgtg accccgtgaa aggaatcgtc
     3061 accgaacagc cggtaggatg ggtatagatg agtctcgaca ccatcaccga tccactgcgc
     3121 ggagcggtag agcacgaccc cggaagcgct ctcgatcagc aacggcaatt cggcgagctt
     3181 ctcgatgcgc tgcaaaccgt cgaccgcgta ggcgacgatg tgcaggtagg ccttcggttc
     3241 cagcgcgccg accggaacca gcacctcggc aaacaactgg ccttcggaac tgcaaagcgc
     3301 gtactcgggt ttcaacagcc ggatcagcga gccgccgatc gacgacagca ccgtcgggca
     3361 accgctgtag ccgtgcagac cgtcgtccga actctgcgcg aaaatttcac ccgacagatt
     3421 gcgaaccacg atcgttttca ggcggaacat gccggaggca acctggtagc gggtgtaact
     3481 ctcggtcagc caggcttcca tttgcgccgg gtcctgccgc gccagcgctt ccctgaacgg
     3541 agcctcgctc tgcagcttga aggcgaactc cttctgccga tcgaaaagcc cgtcgatggc
     3601 ttcgtcgctc tcgtgctcga gcatttcctg cagcgcctcg acctgcgcct cgatggcgtg
     3661 gccgcggtag acataggtgc tgaatatcgc tagcccgtag gccagcgcgc cgagccaaag
     3721 cagcgcgaat ccgataatga atttaaaact gtagatacgc gatagcattc cattactcga
     3781 tcacgtaggg atagtctttg cttttccatt cgacgaaacc gtcgcgtagc cagaacagcc
     3841 gcttgtaacc acattcggat gcgattcgga tggcttcgat gctggtatcc cccgtgctgt
     3901 tattgctgta gaaaacaata gctcgttcca tggaatttgc cagtatgctc agggatttgc
     3961 agttggtgct ggcgagtggc aaattgaccg aggtctcgat gtggccgagc acgtagtcct
     4021 cgacatgacg ggagtcgatg atcaacagcc tggatacata ctggtagagt tcgatcaggt
     4081 gctcggcatc gatggtgacg acaaccgccg tctggccggc ggactcgggc cgttcggcaa
     4141 tcggtgtcgc cagtgcttcc gcacaaaaaa tcagtgccgc gccatacaat gcggccggcc
     4201 gcgccagcgg caaccggccg ggtatgattc cagtcattgt gtagcctacc cggatcgaat
     4261 ccggtttgaa ggttatcgtc agttaactct tggtaatcct tatgttagac gatatttgca
     4321 gaatttctgt cagcaggtga ccccggtacc gccgagaccg caatagccgg ccgggttttt
     4381 ctccaggtac tgctgatggt agtcctccgc gtagtagaaa ggaccggcgg gtaaaatttc
     4441 ggtagtgatc gatccgtatc ccgaggcctg caatttttgc tggtagtatt cgcggcttct
     4501 ttcggcagcc ttgcgctgat tttcgtctag gtaataaatg ccggagcggt actgggtacc
     4561 ctggtcgttg ccctggcgca tgccctgtgt cggatcgtgt gattcccaga agattttcag
     4621 cagcgtgtcg aagtcgattt cccgcgggcg gaaaaccacc agcacgactt cgttgtgccc
     4681 ggtcatgccg gtacagactt cctggtaggt cggattcgga gtatgcccgg cagcataacc
     4741 caccgcggtg gtatgtattc cgcgcaggct ccagaacttg cgctccgcac cccagaaaca
     4801 accgaggccg agaactattt gctcacaatc ctcgggaaac ggcgccacca tcgacgtgcc
     4861 cagcacggcg tgaagcgggt cgaactccat tgcccgctcg cggccaggca gggcttcggc
     4921 ggcactcggt attacgctgg gtcggccaaa cattactttc tcctgtcagt caaaatcaga
     4981 cgcgcgctca ggcacggcgt gtcgaatcca gctcgatcca tgcgggtg
//
LOCUS       DATCZV010000082         3319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_59074, whole genome shotgun sequence.
ACCESSION   DATCZV010000082 DATCZV010000000
VERSION     DATCZV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59074"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VIW27_01845"
     CDS             complement(<1..325)
                     /locus_tag="VIW27_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5738432.1"
                     /translation="MIQSPRRRRGGSRAAKLETRRKPPAINPAPPGAVGGRYRPLNDA
                     QVRQIYDTSLRMLAELGMGEAPPPLVEQALARGATQNQLGRLCFSRDLVEDIIDGACK
                     SFVLHG"
     gene            complement(322..2736)
                     /locus_tag="VIW27_01850"
     CDS             complement(322..2736)
                     /locus_tag="VIW27_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5738433.1"
                     /translation="MKSQARVVVIGGGIAGCSTLYHLTREGCSDVVLVERDELTSGST
                     WHAAAQVTQFGANQTMVALKRHSVNLYRELAADPANPISYHITGGMRLAHTPAQVDTY
                     RHFVGMAAGMGVEMEFIDTAEVGRRHPLINTDGLLGAWWDPLDGDIDPAGITFALARK
                     AREAGAEVYRFNPVENISRKPNGEFVVHCKNGDITCEKVVNATGYRVNEVGRMLGVSH
                     PVTSMEHMYFLTDTMPELEALDFRVPIIRDPGDDFYSRQEKQGLLVGVYEQGCKTFGM
                     DGIDPNFTQALCAPDLDRCLDNMERIFERLPALQEAGIHTVINGPITYTVDGAPLIGP
                     IPGIDNAYCAIGLRAGIGEAGGHGKLLAQIIVHGECEWDAWFLDPRRFTQYANTEHTC
                     LKAIEDYRHEFHYHLPHEHRPAARLARTTPLYPVLDGLNAAWGVVNGWERALFFKPAA
                     DFVDQPGYRFTPTLDVVGKEVEHLQQKVGLMEVSGFTRYEIEGDGVEAFLEHMICGRL
                     PTETGKLGLCYLLTEQGNLLSEATLVKLDENRYWWGSAAAAEWHDRDWLNRFRPDSVS
                     LRELTSTHTILVLAGPRSRALLQSLSPRCDWSATALPWLRAQPMFIGHAEVLAMNLSF
                     SGELAYELHVPNEQLCLVWKLLTDAGTDFGLGHFGLYATESMRLEKGYLHWKADVIYE
                     HNPFEARLERFVDTDKADFVGKAALLEQAARGPRKLLVSMTVDCDIAAAHGGDPVFAN
                     GQQVGSVTSAGYGHRVAKNIAYAYVDPDVAATGTALELGILGERYAATVVDPVLYDPQ
                     YRLPRS"
     gene            complement(2996..>3319)
                     /locus_tag="VIW27_01855"
     CDS             complement(2996..>3319)
                     /locus_tag="VIW27_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009817816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5738434.1"
                     /translation="AVYWSGRPVEDAMFSTAYKSGVSWNDTFWSNARFDELLIKARAE
                     LDEDKRRAMYYEMQELVNMDGGLIAPMFASYVFATSNEVDHGGEFGSNWDVDGERWTE
                     RWSFA"
BASE COUNT          585 a         1053 c         1072 g          609 t
ORIGIN      
        1 gaccatgcaa cacgaaggac ttgcaggcgc cgtcgataat gtcctcgacc aggtcgcgcg
       61 aaaagcacag ccggccgagt tggttttgcg ttgcgccgcg cgccagggcc tgttcgacca
      121 gcggcggtgg cgcctcgccc atccccagtt ccgcgagcat gcgcagcgag gtatcgtaga
      181 tttgacgaac ctgcgcgtcg ttcagcggcc ggtagcgacc gccgacggcg cccggcggcg
      241 ccgggttgat cgccggcggc ttgcgccggg tctccagctt ggcggcacgg ctgccgcccc
      301 tgcgtcttct cggcgactgg atcatgaacg cggcaaccgg tattgcggat cgtagagcac
      361 cgggtcgacg accgtcgcgg catagcgctc gccgagtatg ccgagttcca gcgcggtgcc
      421 ggttgcggcc acgtcggggt cgacataggc gtaggcaata ttcttcgcca cccgatgccc
      481 gtagcctgcg gaagtgaccg agccaacctg ctgtccattt gcaaataccg ggtcgccgcc
      541 atgggcggca gcgatgtcgc agtcgacagt catcgacacc agcagcttgc gcggtccgcg
      601 cgcggcctgc tcgagcagcg cagccttgcc gacgaaatcc gccttgtcgg tatcgacgaa
      661 gcgctcgagc ctcgcctcga acgggttgtg ctcgtaaatg acgtcggcct tccagtgcag
      721 atagcccttc tcgaggcgca tcgactcggt cgcgtaaagc ccgaaatgtc cgagcccgaa
      781 gtcggtaccg gcatcggtga gcagtttcca gaccaggcag agttgttcgt tgggcacgtg
      841 cagttcgtag gcgagttcgc cggaaaaact gaggttcatc gccagcacct cggcgtgacc
      901 gataaacatc ggctgcgcgc gcaaccacgg caacgcggtg gctgaccagt cgcagcgcgg
      961 cgacagcgac tgcagcagcg cgcgcgaccg cgggccggcg agcaccagga tagtgtgcgt
     1021 cgaggtcagc tccctgagcg agacggaatc gggccgaaag cgattcaacc agtcgcggtc
     1081 gtgccattcg gcggcagccg ccgaacccca ccagtagcgg ttctcgtcga gcttgaccag
     1141 cgtcgcctcg ctgagcaggt tgccctgctc ggtcagcagg tagcacaggc ccagcttgcc
     1201 ggtctcggtc ggcagccggc cgcagatcat gtgctcgaga aatgcctcga cgccgtcgcc
     1261 ctcgatctcg tagcgcgtga acccggatac ttccatcagg ccgacctttt gctgcaaatg
     1321 ttcgacctcc ttgccgacca cgtcgagagt cggggtgaag cggtagccgg gctggtcgac
     1381 gaaatcggcc gcgggcttga agaacagcgc ccgctcccag ccgttgacga cgccccaggc
     1441 cgcgttcaac ccgtcgagta ccgggtagag cggggtagtg cgcgcgagcc gcgccgcggg
     1501 tcggtgctcg tgcggcaggt gatagtggaa ttcgtgacgg taatcctcga tcgccttcag
     1561 gcaggtgtgc tcggtattgg cgtattgcgt gaagcgccgc gggtcgagga accacgcgtc
     1621 ccactcgcac tcaccgtgaa cgatgatttg cgccagcagc ttgccgtgac cgccggcttc
     1681 gccgatgccg gcgcgcaggc cgatcgcgca ataggcattg tcgatccccg ggatcgggcc
     1741 gatcagcggc gcgccatcga cggtgtaggt gatcgggccg ttgatcacgg tgtggatgcc
     1801 ggcttcctgc agcgccggca ggcgctcgaa aatgcgctcc atattgtcga gacaacgatc
     1861 gagatccggc gcgcatagcg cctgggtaaa attcggatcg ataccatcca tgccgaaggt
     1921 tttgcagccc tgttcgtaaa cgccaaccag caggccctgt ttttcctgac ggctgtagaa
     1981 gtcgtcgccg gggtcgcgga tgatcggcac gcggaaatcc agtgcttcga gttcgggcat
     2041 ggtgtcggtc agaaaataca tgtgctccat cgacgtgacc gggtgcgaaa cgccgagcat
     2101 gcggccaact tcgttgaccc ggtaaccggt cgcgttgacg actttctcgc aggtgatgtc
     2161 gccattcttg cagtgcacga caaactcacc gttcggcttg cggctgatat tctcgaccgg
     2221 gttgaagcga taaacctcgg cgccggcttc gcgcgccttg cgcgccagcg caaaggtgat
     2281 accggcggga tcgatatcgc cgtccagcgg atcccaccac gcgccgagta ggccgtcggt
     2341 attgatcagc gggtggcgcc ggccgacttc ggcggtgtcg atgaattcca tttccacgcc
     2401 catgccggcc gccatgccga cgaaatgccg gtaggtatcg acctgcgccg gggtatgcgc
     2461 gagccgcatg ccgccggtga tgtggtagga aatcgggttt gccgggtcgg cggcgagttc
     2521 gcggtagagg tttacactgt ggcgcttgag cgccaccatg gtctggttgg cgccgaactg
     2581 ggtcacctgc gccgcggcat gccaggtcga tcccgaggtc agctcgtcgc gctccaccag
     2641 caccacgtcg ctgcagccct cgcgggtcag gtggtaaagc gtcgagcaac cggcgatacc
     2701 gccgccgatg acgactacgc gagcctgtga tttcatcgct tctctccgca ggtcaagatc
     2761 cgagccggct ttcaatgatg ccgaagcggt ttgctttgac aatcgaaaag atatttcaaa
     2821 aaatcaaatt tctttaaccg tcgcccgaca gcaggcattc gtattgcatg atctggcgcg
     2881 tcgatttgcc ggtattcggc ccgcggatga ccggtgacgc accgttaccg gtgcaaccgt
     2941 accccgccgg cggcaggccg cgggcacatc cctgtagccc gcgctgagct gctgcctagg
     3001 cgaacgacca gcgctcggtc cagcgctcgc cgtcgacgtc ccagttggag ccgaattcgc
     3061 cgccgtgatc gacctcgttg gaggtcgcga acacgtagga ggcgaacatc ggcgcgatca
     3121 ggccgccgtc catgttgacg agttcctgca tctcgtagta catcgcgcgg cgcttgtcct
     3181 cgtcgagttc ggcgcgcgcc ttgatcagca attcgtcgaa acgtgcattc gaccagaagg
     3241 tatcgttcca cgacacgccg gacttgtagg cggtcgaaaa catcgcgtct tcgaccgggc
     3301 gcccgctcca gtagaccgc
//
LOCUS       DATCZV010000083         3833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_270573, whole genome shotgun sequence.
ACCESSION   DATCZV010000083 DATCZV010000000
VERSION     DATCZV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270573"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VIW27_01860"
     CDS             complement(<1..674)
                     /locus_tag="VIW27_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HEY5738435.1"
                     /translation="MFETGQKVVSKSWQPELDRGRHYRARLGFILMSTDLAAEADFFA
                     MAPAGVAVHITRLKTDDYTTNETLARHIEHMADAAARIQPDTRPNVISYSCTSGSIVI
                     GEQRVMEEIVAGAPWAQPMCIVTGVVDALRELNARRIVIGTPYLDEINTAEAEYLTAR
                     GFEILDIQGLNLTTGIEFGCVTPAYWKDFALAIDRPDADAIFLSCGGIRALEVADEIE
                     QAAGKPV"
     gene            complement(684..1829)
                     /locus_tag="VIW27_01865"
     CDS             complement(684..1829)
                     /locus_tag="VIW27_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5738436.1"
                     /translation="MFSRRLDDLRRRLHENGLDAAVITDDDNVYYLTGYYDYLHMEFG
                     RPTLLVVPKHGDSLLITPTIDLAMAESSARVDRIAAWNDGAGNEWREELPAMLRGLQS
                     VAIEPQHIPPPVRAYLDSLLDGTALRDLTPLLAAMRMIKSAEELQLARHAGEVAGAMM
                     SAGREAIADGVAEFEVALATSQAGTRKAAELLAAHYQDRAMSPNTHFLQIMASGEEIV
                     KTHHRASTRIMRHGEPVFLCFCGMTNFHRFKLGFDRTFWIGEIADPQQAGIYEVAVAS
                     QQAALAALKPGVTAESVHAAYAEVIQQAGFDYPFRCGRATGFSFLEAPQLVTGDRTMP
                     GCSRVLAVDGSVSTSNFRAQVGDSFILTDDGYEQITRHPKSIDAMVL"
     gene            complement(1830..2999)
                     /locus_tag="VIW27_01870"
     CDS             complement(1830..2999)
                     /locus_tag="VIW27_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019275085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY5738437.1"
                     /translation="MLTDKYHAAMQEWRHDIHRYPELAFEERRTADKVAALLQEFGLE
                     VHRGIGETGVVGILRRGQSTATIGLRADMDALLIEERNEFDHRSREHGKMHACGHDGH
                     TAMLLGAARYLAQEGDFDGTAVFIFQPAEEHGAGARAMIDDGLFERFPVDSIYAIHNF
                     PSLETGRFAVRAGPIMAAEDNFEITVRGVGHHAAMPHLGRDPLVVGAEIVTAMQSLVS
                     RRVDPMANAVVSFTEFVTDGTVNVVPSNVVLKGDTRSLTLEVQDLIETTMGRVVAGIC
                     SAHGVEHEFSYRRNFVPTINTAAEAGIAARVAASVAGADNVTDKTGPVMTSEDFGYML
                     QARPGAYILLGNGKDGVGGCSLHNPHYDFNDEILAIGADFWVALVESELTAERSA"
     gene            complement(3074..>3833)
                     /locus_tag="VIW27_01875"
     CDS             complement(3074..>3833)
                     /locus_tag="VIW27_01875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY5738438.1"
                     /translation="VVELTSGNMGTGLAIVCTLRGYPFVAVMSRGNSIERARMMTALG
                     AEVVLVEQAPASLPGQVSGADLQRVEAVAARITRERGAFRADQFARDGNPLAHYRHTG
                     PEIWRQSGGCVTHFCDFVGSGGSYAGIARYLKQQDEAIGCYVVEPERATALASGRVDN
                     PDHPIQGGGYSMTSLAFLDDVPVDGYLGVSGERARDCARELARREGIFGGYSAGANVA
                     AALELLRGPARGGVVAVLICDSGLKYLSTDLWDC"
BASE COUNT          683 a         1261 c         1208 g          681 t
ORIGIN      
        1 accggcttgc cggccgcctg ctcgatttca tcggcgacct cgagcgcgcg aataccgccg
       61 caactgagaa agatcgcatc cgcatcgggc cggtcgatgg cgagcgcgaa atccttccag
      121 taggccggcg tcacgcagcc gaactcgatg ccggtggtca gattgagacc ctggatgtcg
      181 aggatttcga aacccctggc ggtcaggtat tccgcttctg cggtattgat ctcgtcgagg
      241 tacggcgtac cgatcacgat tcggcgcgcg ttcagttcgc gcagcgcatc gacgacgccg
      301 gtgacgatac acatcggctg cgcccacggc gcccctgcga cgatctcttc catgacccgc
      361 tgctcgccga tgacgatcga gccgctggtg cagctatagc tgatcacgtt cggtcgcgta
      421 tcgggttgaa tgcgcgccgc ggcatcggcc atatgctcga tatggcgcgc aagcgtctca
      481 ttggtggtgt aatcgtccgt tttcaggcgc gtgatgtgta ccgcgacgcc cgccggcgcc
      541 atcgcgaaga aatcggcctc ggccgccaga tccgtggaca tcaggataaa accgagccgc
      601 gcgcggtagt gcctgccgcg atccagctcc ggctgccagg atttcgatac caccttctgc
      661 cctgtctcga acatcgaaag ctcctataac accattgcgt cgatggactt gggatgccgg
      721 gttatctgtt cgtaaccgtc atcggtaaga ataaagctgt cgcccacctg ggcgcggaaa
      781 ttgctggtcg agaccgagcc gtcgacggcc agcaccctgc tgcagccggg catggtgcgg
      841 tcgccagtca ccagctgcgg ggcctcgaga aagctgaaac cggtcgcccg gccgcagcga
      901 aacgggtaat cgaagccggc ctgctgaatg acctcggcgt aggcggcgtg gaccgattcc
      961 gcggtaaccc ccggtttcag cgccgccagc gccgcctgct ggctggcgac cgcgacctcg
     1021 tatatacccg cctgttgcgg atcggcgatc tcgccgatcc agaaggtgcg atcgaaaccc
     1081 agcttgaagc ggtggaaatt ggtcatgccg cagaaacaca ggaataccgg ctcgccgtgg
     1141 cgcatgatac gggtactcgc gcggtgatga gttttgacga tttcctcacc agaggccatg
     1201 atctgcaaaa agtgggtatt gggcgacatc gcccggtcct ggtaatgcgc cgcgagcaac
     1261 tcggccgcct tgcgggttcc ggcctgcgag gtcgccagcg cgacttcgaa ctcggcgaca
     1321 ccgtcggcga tcgcttccct gccggcgctc atcatcgcgc cggcgacttc gcccgcatgg
     1381 cgcgcgagct gcaattcctc ggccgacttg atcatccgca ttgctgccag cagcggcgtc
     1441 agatcgcgca gcgccgtgcc gtccagcagg ctgtccaggt aggcacgcac cggcggtggt
     1501 atgtgctgcg gctcgatggc cacgctctgc aagccccgca gcatcgccgg caattcctca
     1561 cgccactcgt tgccggcacc gtcgttccag gcggcgatgc gatcgacgcg cgcgctcgat
     1621 tcggccatcg ccaggtcgat ggtcggcgtg atcagcaggc tgtcaccgtg tttgggcacc
     1681 accagcagcg tcggccggcc gaattccatg tgcaggtaat catagtagcc ggtaaggtaa
     1741 tacacgttat cgtcgtcggt gatcaccgcc gcatcgaggc cgttctcgtg caggcggcgc
     1801 cgaaggtcgt cgagccgtcg gctgaacatt tacgccgagc gctccgcggt gagctcggat
     1861 tcgaccagcg cgacccagaa atcggcaccg atggcgagga tctcgtcgtt gaagtcgtaa
     1921 tgcgggttgt gcaggctgca accgccgacg ccgtccttgc cgttgccgag caggatataa
     1981 gccccgggcc gcgcctgcag catgtagccg aaatcttcgg aggtcatcac cggcccggtt
     2041 ttatcggtga cgttatcggc gccggccacc gaggcggcga cgcgcgcggc gatgccggcc
     2101 tcggcggcgg tattgatcgt cggcacgaag ttgcgccggt aactgaattc gtgttcgacg
     2161 ccgtgcgcgc tgcagatgcc ggcgaccacc ctgcccatgg ttgtttcgat caggtcctga
     2221 acttccagag tcagcgagcg cgtatcgccc ttcagcacca cgttcgatgg caccacgttg
     2281 acggtgccgt cggtaacgaa ctcggtaaac gagaccaccg cgttggccat cgggtcgacg
     2341 cgccgcgaca ccagcgattg catcgcggtg acgatttcgg cgccgacgac cagcggatcg
     2401 cgccccaggt gcggcatcgc cgcgtgatgg ccgacaccgc gcacggtgat ttcgaaattg
     2461 tcttccgctg ccatgatcgg gccggcacgg accgcgaaac ggccggtctc gagcgatgga
     2521 aaattatgaa tcgcgtaaat cgagtccacc gggaagcgct cgaacaggcc gtcgtcgatc
     2581 attgcccgag cgccggcgcc gtgctcttcg gcgggttgaa agatgaatac cgcggtaccg
     2641 tcgaaatcgc cctcttgcgc caggtaacgc gccgcgccga gcaacatcgc ggtgtgaccg
     2701 tcgtgtccgc aggcatgcat tttgccatgc tcccgggagc gatgatcgaa ttcattgcgc
     2761 tcctcgatca gcagcgcgtc catgtcggcg cgcagaccga tcgtggctgt gctctggccg
     2821 cgccgcagaa tgccgacgac accggtctcg ccgataccgc gatgcacctc gagaccgaat
     2881 tcctgcagca gcgcggcgac cttgtcggcg gtgcgccgct cctcgaaagc cagctccgga
     2941 tagcggtgga tgtcgtggcg ccattcctgc atcgctgcgt gatatttgtc ggtaagcatg
     3001 gtcgggggcc gttggtattt acttcccgga gccgaaaata ccgcttaaat ccgccaaaga
     3061 ccagcgttaa atcctagcag tcccaaaggt cggtggagag gtacttcaga ccgctgtcgc
     3121 aaatcagcac cgccaccacc ccgcccctgg ccggcccgcg caacaattcg agcgccgcgg
     3181 caacgttggc accggctgag taaccgccga aaataccttc gcgccgagcc agttcacgcg
     3241 cgcaatcgcg cgcccgctcg ccggaaacgc ccaggtagcc gtcgaccggc acgtcgtcca
     3301 ggaacgccag cgaagtcatc gagtaacctc cgccctggat cgggtgatcg ggattgtcta
     3361 cccggccgct tgccagtgcc gtcgcgcgct ccggctcgac cacgtaacag ccgatcgcct
     3421 cgtcttgctg cttgagatat cgcgcgatac cggcatagct gccgcccgag ccgacgaaat
     3481 cgcagaaatg ggtgacgcag ccgccggact ggcgccagat ctccggcccg gtatgccggt
     3541 agtgagcgag cggattgcca tcacgggcga actggtcggc gcgaaacgca ccgcgttcgc
     3601 gggtgatacg cgcggcgacg gcttcgacgc gttgtagatc ggcgcccgag acctgcccgg
     3661 gcagcgaagc cggcgcctgc tcgaccagta ccacctcggc gccgagcgcg gtcatcatgc
     3721 gcgctcgctc gatcgagttg ccgcgcgaca tcacggcgac gaacgggtag cccctgagcg
     3781 tgcagacgat cgccagcccg gtgcccatgt tgcccgaggt cagctcgacc acg
//
LOCUS       DATCZV010000084         5335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_270587, whole genome shotgun sequence.
ACCESSION   DATCZV010000084 DATCZV010000000
VERSION     DATCZV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270587"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..739
                     /locus_tag="VIW27_01880"
     CDS             173..739
                     /locus_tag="VIW27_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009723219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY5738439.1"
                     /translation="MREAQSSKGGVAGLLDTIDGIIARIEEVMMALGVILMAVNTIAN
                     VISRFIFNHSIIFAEELNSIFILLVTFAGVGYAARHGRHIRMSAIYDAMPDKVRKIMM
                     IVITGVTAFCMLFLAWYSVEYILSIQSKGRIMPALGVPVYLVYLWVPVGFFVTGVQYA
                     LTMVKNVREKEIYLSTNLHEHEAQEIEV"
     gene            742..2028
                     /locus_tag="VIW27_01885"
     CDS             742..2028
                     /locus_tag="VIW27_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY5738440.1"
                     /translation="MALAIFLVMLVLLLLGFPMMIPLIIGAFVGVVSLFGDIGRTEFM
                     VQQMLGGIRPASLIAVPMFILAADIMTRGHSADRLVDLVMAFVRHIKGGLAVSTSGAC
                     ALFGAVSGSTQATVVAVGGPLRPRMLKGGYDNSFVLALIVNSSDIAFLIPPSIGMIIY
                     GVVSKSSIPELFIAGIGPGLLILVLFSIYSMIYATVKKVPTEEPASWKERFDMIKRAG
                     WPLGFPVIIVGGIFGGIFSPTEAAAVSVLYALFLETIVFKSITIRDFYSIAKSTGLVT
                     GVVFILVGAGAAFAWVLSFAQIPQAILSGIGIDQMGPTGVLFVIAIAFFIGCMFVDPI
                     VVILILVPIFAPVIKSTGLDPILVGTLITLQAAIGSATPPFGCDIFTAVAVFKQPYLE
                     VIRGTPPFVFILLLVAVLLIFFPEIALYLRDVAFDK"
     gene            2041..2478
                     /locus_tag="VIW27_01890"
     CDS             2041..2478
                     /locus_tag="VIW27_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5738441.1"
                     /translation="MFKTILVPVDGSEAAHAALDQAIELQKLCDAELLILSVYREHNL
                     WKASMTMVRDDLTRSTDEALEEFAREVAERSKAYVKSKGVEKVRSFYMGGGPARMIVK
                     FSEDHGVDLIVMGSRGMSDSTRYLLGSVSHKVTSLAQCPVMVI"
     gene            2524..4014
                     /locus_tag="VIW27_01895"
     CDS             2524..4014
                     /locus_tag="VIW27_01895"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5738442.1"
                     /translation="MSLDQLPRSGDKSSNLLHDASLWREDAYVDGAWCAAKGDARFDV
                     TDPADGARLASVASLGAAQSRAAVEAAQAAFPDWSMSLPQERAQILRRWYDLIVEHRE
                     DLALIMTLEQGKSLDESRGEIDYGASFVEFYAEEAKRPNIEGVTSHLKGAEVEVWREP
                     VGVAGLITPWNFPHAMLTRKAAAALAAGCTVVAHPSHETPLSALALAELAVRAGFPRG
                     VFNVLPGSARVIVAEWMATPTVRAISFTGSTEVGRLLFRQSADTVKRMVMELGGHAPF
                     LVFADAELERAVECAISAKFATSGQDCLAANRILVERPVYDEFCARFAQKTAALTVGR
                     GLDNAAIGPLMNLDALNKQIEQVEDAVASGARLLTGGAKHDAGELFYQPTVLADVPAE
                     ARIFHEETFGPVAGIAPFDSEDEALARANDTEYGLVAYVHAENAHRIYRLTRALQYGM
                     VAVNRTKVTGAPIPFGGMKQSGLGREGARLGMEAFTEVKYVCRDYA"
     gene            4044..>5335
                     /locus_tag="VIW27_01900"
     CDS             4044..>5335
                     /locus_tag="VIW27_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5738443.1"
                     /translation="MNNNAIIEPGKLQSALTNDEISRMDRATNLHPFTPLRAFEADEL
                     GGPRIVESGQGIRITDAEGKTSIDGFAGLYCVNIGYGRTEVADAIARQAHQLAYYHCY
                     AGHTTRELARLSQRLVERAPGNVKKVFYGLSGSDANETQAKLVWHYHNLIGKPDKKKI
                     IARERGYHGCSVVSGSMTGMNFYHAHMDLPVKSILHTGVPHYYWGAEADESELEFSRR
                     RAAELEQMILAEGPDTVGAFIGEPVLGTGGIIPPPEGYWDEIQNVLRNYDVLLIVDEV
                     VTAFGRIGAWFGCERYGIEPDLITVAKGLTSAYAPLSAVLIGERVTDALLAHSDEIGA
                     FSHGYTYSGHPICVAAANANLDVLENDRIVEHVAGLGDALRDTLADAVAESPIVGEVR
                     GTGMLAAIEFVADPANKTRFDASHKVGPRMSAACLANG"
BASE COUNT          957 a         1637 c         1676 g         1065 t
ORIGIN      
        1 catcgcgcag atgaaaaagg acctgaaggc ggccgggcag taagccaaga aactttcctg
       61 gagaattgcc ggggatttac tcctccttcc gttacggggt ctcgacggga ccccgtaacg
      121 cctgtctccg gtcagtcttt gtttcaacta accaccgatc cgggaataga tcatgagaga
      181 agcccaaagc agtaaaggcg gggtcgccgg cctgctcgac acgatcgacg gcattatcgc
      241 caggatcgaa gaggtcatga tggcgctcgg cgtaatcctg atggcggtta ataccatcgc
      301 taatgtcatc agccgtttca tcttcaacca ttcgatcatt ttcgcggagg agctcaatag
      361 catttttatc ctgctggtga ccttcgccgg cgtcggttac gcggcgcgcc acggtcggca
      421 cattcggatg tcggcaatat acgacgccat gccggacaag gtgcgcaaga tcatgatgat
      481 cgtgatcacc ggcgtcaccg cattctgcat gctgttcctg gcctggtatt cggtggagta
      541 catcctgagc atccagtcca agggccgcat catgcccgcg ttgggagtgc cggtttacct
      601 cgtttacctg tgggtgccgg tcgggttttt cgtcaccggc gtgcagtacg cgctgacgat
      661 ggtcaagaac gttcgggaaa aggaaattta cctttcgacc aacctgcacg agcacgaagc
      721 gcaagaaatc gaggtttagc catggcgctc gcgatattcc tggtcatgct ggtgctgcta
      781 ttgctcggtt ttccaatgat gataccgctg atcatcggcg cattcgtcgg cgtcgtgtcg
      841 ctgttcggcg atatcggccg taccgagttc atggtgcagc aaatgctcgg cggtatccgc
      901 ccggcgtcgc tgatcgcagt accgatgttc attctcgccg ccgacatcat gacccggggc
      961 cattccgccg accgcctggt cgacctggtg atggccttcg tgcgccacat caagggcggg
     1021 ctcgcggtca gcacctcggg cgcttgcgcc ctgttcggcg cggtatcggg atcgacccag
     1081 gcgaccgtgg tcgcggtcgg tggcccgttg cggccgcgca tgttgaaggg cggttacgac
     1141 aattcattcg tgctcgcgtt gatcgtcaac tcgagcgata tcgcgtttct gatcccgccg
     1201 agcatcggca tgatcatcta cggcgtggtg tccaagtcgt cgattccgga actgttcatc
     1261 gccggtatcg ggccgggcct gctgatcctc gtgctatttt cgatttacag catgatttac
     1321 gccacggtca agaaagtccc gaccgaggag ccggcgagct ggaaggagcg cttcgacatg
     1381 atcaagcgcg cgggctggcc gttgggcttt ccggtgatta tcgtcggcgg cattttcggc
     1441 gggatcttca gcccgaccga ggccgccgcg gtcagcgtgc tttacgcgct gttcctcgaa
     1501 accatcgtgt tcaagtcgat cacgatccgc gatttttatt ccatcgcgaa gtcgaccggt
     1561 ctcgtcaccg gggtggtctt catcctcgtc ggcgccggcg ccgccttcgc ctgggtgcta
     1621 tccttcgccc agattccgca ggctatcctc agcggtatcg gcatcgatca aatgggaccg
     1681 actggcgtgc tgttcgtgat cgcgatcgcg tttttcatcg gctgcatgtt cgtcgatccc
     1741 atcgtcgtca tcctgatcct ggtgccgatt ttcgcaccag tgatcaagag caccggtctc
     1801 gatccgatcc tggtcggcac gctgatcacg ctgcaggccg cgatcggctc ggcgacgccg
     1861 ccgttcggtt gcgacatctt caccgcggtg gcggtgttca aacagcctta cctggaggta
     1921 attcggggca cgccgccgtt cgtgttcatc ctgctgctgg tcgcggtact gctgatcttc
     1981 tttcccgaaa tcgcgctcta cctgcgcgac gtcgctttcg acaagtaatg gagggcgggc
     2041 atgtttaaaa cgatcctggt acccgtggac ggctccgaag ccgcgcacgc cgcgctcgac
     2101 caggcgatcg agctgcaaaa gctgtgcgat gccgaactgt tgattctgtc ggtttaccgc
     2161 gagcacaacc tgtggaaagc ctcgatgacg atggtgcgcg acgacctgac ccgatcgacc
     2221 gacgaagcgc tcgaggaatt cgcccgggaa gtcgccgagc gcagcaaggc ttacgtaaag
     2281 tcgaagggtg tggagaaagt tcgttcgttc tacatgggcg gcgggcccgc gcgcatgatc
     2341 gtcaagttca gcgaagacca cggcgtcgac ctgatcgtca tgggcagccg cggcatgagc
     2401 gactcgaccc gctacctgct cggcagcgta tcgcacaagg ttaccagcct ggcgcagtgc
     2461 ccggtaatgg tgatctgatc ggggggcttc ctgtcaataa aatgaaacga gaggttaatc
     2521 gaaatgtcgc ttgatcaact gccccggtcc ggtgataaaa gttcgaattt gctgcacgac
     2581 gcatccctgt ggcgtgaaga tgcctacgtc gacggcgcct ggtgtgccgc taagggcgac
     2641 gcccgtttcg acgtcaccga tccggccgac ggcgctcggc tggcgtcggt cgcgagtctt
     2701 ggcgcggcgc agagccgggc cgcggtcgaa gccgcgcagg ccgcctttcc cgattggtcg
     2761 atgtcgttgc cgcaggagcg cgcgcagatt ctgcgccgct ggtatgacct gattgtcgag
     2821 caccgggaag acctggcgct gatcatgacg ctggaacagg gcaagtcgct ggacgagtcg
     2881 cgtggcgaaa tcgattatgg cgcgtctttc gtcgagtttt acgccgagga agccaagcgg
     2941 ccgaacatcg agggcgtgac atcgcacctg aagggcgcgg aggtcgaggt ctggcgagaa
     3001 ccggtgggtg tcgccgggct gatcacgccg tggaattttc cgcacgcaat gctgacccgc
     3061 aaagccgcgg ccgcgctcgc cgcgggctgt accgtggtcg cgcacccgtc gcacgaaacg
     3121 ccgctgtcgg cgctcgcgct tgccgaactc gccgtccgag ccgggttccc gcgcggcgtg
     3181 ttcaacgtat tgccgggcag cgcgcgcgtg atcgtggccg agtggatggc gacgccgacc
     3241 gtgcgcgcca tctcgtttac cggatcgacc gaggttgggc gcctgctgtt caggcagagc
     3301 gcggatacgg tcaagcgcat ggtcatggag ctgggcgggc acgcgccgtt cctggtattc
     3361 gccgacgccg agctggaacg cgccgtcgaa tgcgcgatct cggccaaatt tgcgacctcg
     3421 ggccaggatt gcctcgcggc taatcgtatc ctcgtggaac ggccggtgta cgatgaattc
     3481 tgcgcgcgct tcgcgcaaaa aaccgccgcg ctcaccgtcg gccgcggact cgacaatgcc
     3541 gcgatcggac cgctgatgaa tctggatgca ctgaacaaac agatcgagca ggtcgaggat
     3601 gccgtcgcga gcggcgcgcg cctgttgacc ggcggcgcta agcacgacgc cggggaactg
     3661 ttttaccagc cgaccgtgct tgccgacgtg ccggccgagg cacgaatctt tcacgaggaa
     3721 accttcggtc cggtcgcggg cattgccccg ttcgactccg aggatgaagc gcttgcgcgc
     3781 gccaacgata ccgagtacgg cctggtcgcc tacgtgcacg cggagaacgc acaccgcatc
     3841 taccgcctga cgcgcgcgtt gcagtacggc atggtcgcgg tcaaccgcac caaggtgacc
     3901 ggcgcgccga tcccgttcgg cggcatgaag cagtcagggc tcgggcgcga gggcgcccgc
     3961 ctcggcatgg aggcctttac cgaggtcaag tacgtctgtc gcgactacgc ctgacgtgta
     4021 gccgcctttt tacaggaacc gacatgaaca acaatgccat tatcgaaccc ggaaaattgc
     4081 agtccgcgct gaccaatgac gagatttcgc ggatggatcg tgccaccaac ctgcacccgt
     4141 ttaccccgct gcgtgccttc gaggccgacg aactcggcgg gccgcgcatc gtcgagagcg
     4201 ggcaggggat tcgcatcacc gatgccgagg gtaaaacctc gatcgacggc ttcgccgggc
     4261 tctattgcgt caacatcggt tacggccgca ccgaggtcgc cgatgcgatc gcgcgccagg
     4321 cgcatcagct cgcctattac cactgttatg ccgggcacac cacgcgcgaa ctcgcgcgcc
     4381 tgtcgcagcg cctggtcgag cgcgcgccgg gtaacgtgaa aaaggttttc tacggcctgt
     4441 ccggttcgga cgccaacgaa acccaggcca agctggtgtg gcattaccac aacctgatcg
     4501 gcaagccgga caagaaaaaa attatcgcgc gcgaacgcgg ctaccacggc tgttcggtgg
     4561 tctcgggttc gatgaccgga atgaattttt accacgcgca catggatttg ccggtaaaga
     4621 gcattctgca caccggcgtg ccgcattact actggggcgc cgaggcggac gagtccgagc
     4681 tcgagttttc gcgccggcgc gcggccgaac tcgagcagat gattctggcc gaaggcccgg
     4741 acacggtggg tgcattcatc ggtgaaccgg tgctcggcac cggcggtatc attccgcctc
     4801 ctgagggtta ctgggacgag attcagaatg tcctgcgcaa ctatgacgtg ctgctgatcg
     4861 tcgacgaagt ggttaccgcc ttcggccgca tcggcgcttg gttcggctgc gaacgttacg
     4921 gcatcgaacc cgacctgatc accgtggcca agggcctgac gtcggcttac gcgccgctgt
     4981 cggcggtcct catcggcgag cgtgtcacag acgcgctgtt ggcccattcc gacgagatcg
     5041 gggcgttttc gcacggttac acctacagcg ggcacccgat ctgtgtcgcc gccgccaatg
     5101 ccaacctcga cgtcctcgag aacgatcgta tagtcgagca tgtggccggg ctcggtgacg
     5161 cgttgcgcga cacgcttgcc gacgcggttg ccgaatcacc gatcgtcggc gaggtccgcg
     5221 gcaccggcat gctcgccgcg atcgaatttg tcgccgatcc cgccaacaag acccgcttcg
     5281 acgcgagcca caaggtcgga ccgcgtatgt ccgccgcttg tctcgccaac ggctt
//
LOCUS       DATCZV010000085         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_694433, whole genome shotgun sequence.
ACCESSION   DATCZV010000085 DATCZV010000000
VERSION     DATCZV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694433"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..2430
                     /gene="lptD"
                     /locus_tag="VIW27_01905"
     CDS             253..2430
                     /gene="lptD"
                     /locus_tag="VIW27_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY5738444.1"
                     /translation="MLRLRYLLAIVPLSASFSSMAAENWGLCSVPSFQFIEAEGLAEG
                     ETRLEANQIASENAETMRLLGDVSVVRKRQQLSADEVLLQKQSEHITAIGNALLQQPG
                     NRAISERIEIDNLNDTAVIERPHFELSGRHARGSAEKIVKLDNFRSRFEDLLYTACDP
                     GDRFWHLRAAELEIDDQSGRGSSTHTTLYLQGFPFIYLPYFQFPIDDRRLSGLLSPTY
                     GYSRDDGTTLLLPIYWNQAPNYDMTITPAWFKKRGLQLNTENRYLFESGRGQLDLSWL
                     DDKKLDQTRWFQQWRHQSSLPWDINADLQLAEVSDGKVFDDFENVAPEYNDISHLERR
                     VRFGRSGEVWSGELLWQDYQTLDPTTAIGGRPYRRLPSLKIDARPASWWGGLRTPLSA
                     EITAFDRDDSVTGNRAHLVGSAVWTAEDSWYFFEPQLDLAVTDYRLENTTGDETLHRA
                     LPTLGIDSGLIFERDAGSGGKWLQTLEPRLFFLHTPFEDQDEFPNFDSALKGSTYDNL
                     FSSNRFNGADRIGDASQVTLGLASQLYSSDDGTRLMRVRAGQIHYFEDRRVSLNGTVD
                     DAERSDVIGELDLWPNPRTRVSARLVRDQQTGEYDDRELSMSYRNAGLVANLGYYFTE
                     EELEQALASLVYPLNERWTLVAKYHQSLRFEKPVDHLLGFGYESCCWGFKMLYGESGD
                     EDEDFAETDYRVYFELTLKGLTDAGQDIDLQLGESIPGYQSAF"
BASE COUNT          502 a          821 c          750 g          459 t
ORIGIN      
        1 gtcaatggcc gcgatttcgt gattcactgg gacgcgccca atcgcagcgt caccatcgac
       61 gacaagaatg gcaacgcggt cgccgcgatc cagggattct ggttcgcgtg gttcgcgttt
      121 cacccgcaaa ccctggtgtt cgaatccgcg aacgagtgaa cctcgccggt agcggccgtg
      181 cagcgaaaat gttcagccgg gattaaccgg ggcgcgataa tgtgacaaaa tcgttttatt
      241 ttctggactg tcttgctgcg ccttcgatac ctcctggcca tcgttccgct gagcgcctcg
      301 ttttcgagca tggcggcgga gaactggggc ctttgcagcg ttccgagctt ccagttcatc
      361 gaggccgagg gtctcgccga gggtgaaacc cgcctcgaag ccaaccagat cgccagcgag
      421 aacgccgaga ccatgcgcct gctcggcgac gtcagcgtgg tgcgcaagcg gcaacagctc
      481 agcgccgacg aagtgctgct gcaaaagcag agcgaacaca tcaccgccat cggtaacgcg
      541 ctgctgcagc aacccggcaa ccgcgcgatc agcgagcgca tcgaaatcga caatctcaac
      601 gataccgcgg tgatcgagcg gccgcacttc gagctctccg ggcggcacgc gcgcggttcg
      661 gccgagaaaa tcgtcaaact ggacaacttt cgcagccgct tcgaggacct gctctatacc
      721 gcctgcgatc ccggtgatcg cttctggcac ctgcgcgccg cggaactcga gatcgacgac
      781 cagagcggcc gtggcagctc cacccatacg acgctttacc tgcagggttt cccgttcatc
      841 tacctgccgt atttccagtt cccgatcgac gaccgccgcc tgtccgggct gctgtcgccg
      901 acctatggct acagcaggga cgacggtacc acgctgttgc tgccgattta ctggaaccag
      961 gcacccaact acgacatgac gatcaccccg gcctggttca agaagcgcgg cctgcagttg
     1021 aataccgaga accgctacct gttcgaatcc ggccgcgggc agctcgacct gtcgtggctc
     1081 gacgacaaga aactcgacca gacgcgctgg tttcagcagt ggcgccacca gtccagcctg
     1141 ccgtgggata tcaacgccga cctgcagctg gccgaggtat cggatggcaa agtgttcgac
     1201 gatttcgaga atgtcgcccc cgagtacaac gatattagcc atctcgaacg ccgcgtgcgc
     1261 ttcggccgct ccggcgaggt ctggtccggc gagctgttgt ggcaggatta ccagacgctc
     1321 gacccgacga ccgcgatcgg cggccggcct taccgacggc tgccgagtct gaagatcgat
     1381 gcccgacccg cgagctggtg gggcggactg cgcacgccgc tgagcgccga gatcacggct
     1441 ttcgatcgtg acgacagtgt taccggcaac cgcgcacacc tggtcggctc ggctgtctgg
     1501 acggcggaag acagctggta ctttttcgag ccgcagctgg atctcgcggt caccgattac
     1561 cggctggaga acactaccgg tgacgagacc ctgcaccgcg cactgccgac actcggtatc
     1621 gacagcggcc tgatattcga gcgcgatgcc ggcagcggcg gcaagtggct gcagaccctc
     1681 gagccccggc tgtttttcct gcacaccccg ttcgaggacc aggacgaatt ccccaatttc
     1741 gattccgcgc tcaagggcag tacttacgac aacctgttca gcagtaaccg cttcaacggc
     1801 gccgaccgca tcggcgacgc cagccaggtg accctgggtc tcgccagcca gctttactcg
     1861 agcgacgacg gcaccaggct gatgcgcgtg cgcgccggac agatccatta tttcgaggac
     1921 cggcgggtca gtctcaacgg caccgtcgac gatgcggaac gctccgacgt gatcggcgag
     1981 ctggatttat ggccgaatcc ccgaacccgc gtatcggccc gcctggtgcg cgaccagcaa
     2041 accggggaat acgacgaccg cgagttgtcg atgagttacc gcaatgccgg gctggtcgcc
     2101 aacctcggtt attactttac cgaggaagaa ctcgaacagg cgctggcatc gctggtctac
     2161 ccactcaacg aacgctggac gctggtcgcc aagtaccacc agtcgttgcg gttcgagaag
     2221 ccggtcgacc acctgctcgg tttcggctat gaatcctgct gctggggctt taaaatgctc
     2281 tacggcgaaa gcggtgacga agacgaggat ttcgccgaaa ccgactaccg tgtctatttc
     2341 gagctgaccc tgaagggcct gaccgacgcg ggccaggaca tcgacctgca gctcggcgaa
     2401 tcgattcccg ggtaccagtc ggccttttga cagcaacgcc aagacccatg aagccaatcg
     2461 caacagttct cgttttactc tgcaatctcc tgctctggca gccggcaacc gctgcccagg
     2521 tggtgctgga cc
//
LOCUS       DATCZV010000086         7014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_694434, whole genome shotgun sequence.
ACCESSION   DATCZV010000086 DATCZV010000000
VERSION     DATCZV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694434"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VIW27_01910"
     CDS             <1..362
                     /locus_tag="VIW27_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY5738445.1"
                     /translation="IYDIELEQVTVKPMDDGLANAPIMILMKQSNIDEQFEISEVGQR
                     KYLYWIELKPRSRELEYTDIYIGLEDGRVKAMELQDRFGQSTQIVFDNLRVDVVHDPA
                     TFRFEPPPGVDVYGVGG"
     gene            475..1776
                     /locus_tag="VIW27_01915"
     CDS             475..1776
                     /locus_tag="VIW27_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY5738446.1"
                     /translation="MNDLFAAENAFKPLADRMRPRRLDDVFGQAHLLAPDKPLRQAIE
                     AGKLHSMIFWGPPGTGKTTIARLIAEYSEARFITLSAVLAGVKDIRDAIAQARQFQGL
                     TVLFIDEVHRFNKAQQDAFLPHIEDGTILFVGATTENPSFELNNALLSRTRTYVLKPI
                     SATDIENVVRRALADDASGLGGLGLEIDDDALQMLARAADGDARRALTYLEIVSDLID
                     KGRIDAALVAEIAQQGPRRFDKGGDVFYEQISALHKSVRGSDPDAALYWFCRMIDGGC
                     DPVYIARRVVRMAIEDIGLADPRGLELCLNGWQTFERLGSPEGELAIAQGIVYLACAA
                     KSNAVYAAYKQAMDDARNSGSLEVPLHLRNAPTRFMEEQGYGEGYRYAHDEDGAFAAG
                     EVYFPEQLGAREYYHPVDRGLEIRIAEKLESLRRRNSESKP"
     gene            1773..2360
                     /locus_tag="VIW27_01920"
     CDS             1773..2360
                     /locus_tag="VIW27_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY5738447.1"
                     /translation="MNLPYAEAAEQNKHVIYQAISGYLAGDALEIGSGTGQHAVYFAG
                     RSPAVSWQTSDLGAALPGLEARIRSSGLDNLPPPIELDVTGAWPPRKFALVYTANSLH
                     IMSSAMVEACFAGVGDCLQSGGVFAAYGPFNYQGEYTSPSNAGFDRMLRSRDPSSGIK
                     DYVWLTELAVAARLELLDDIEMPVNNRTLVWQKRT"
     gene            2407..3153
                     /locus_tag="VIW27_01925"
     CDS             2407..3153
                     /locus_tag="VIW27_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY5738448.1"
                     /translation="MNKTVILSAAAGILLLIGASLQAQEPAADDQLTRLLKLRLGSEQ
                     IAPPVETEIDGVYMTRFGNRFAYLLEGGRYIFVGDLVDLEQGRNLTELSRREMVVDEL
                     AAFGSEKRVIFPAVDEEKAVLSVFTDTSCGYCQQLHREVEYLQQAGISVHYIPFPRGG
                     NRGPGYQDLKQVWCSQDPLEAMSIAKKARPGDLDAAENCESAQYVDEGYQLGNRIGVS
                     GTPSLYTADGTHFNGYVPYRELIQRLLMSP"
     gene            3251..3433
                     /locus_tag="VIW27_01930"
     CDS             3251..3433
                     /locus_tag="VIW27_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 4"
                     /protein_id="HEY5738449.1"
                     /translation="MKKNPEAAPKTETKVEVSVDDARLDGPGGDNDVPAEYGGPRGKE
                     PTRYGDWERNGRCIDF"
     gene            3485..3868
                     /gene="sdhC"
                     /locus_tag="VIW27_01935"
     CDS             3485..3868
                     /gene="sdhC"
                     /locus_tag="VIW27_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY5738450.1"
                     /translation="MSLRKHPTSPHLQIYRLPLTALLSITHRITGVLLAVGCVLLIGI
                     LAAAADGAATYEAIVPHLQSWYGQLLLIGLVFSLYLHFCNGVRHLVWDAGYGFELDTV
                     DLTAKLAIALAIALTAATWLVAGSW"
     gene            3873..4253
                     /gene="sdhD"
                     /locus_tag="VIW27_01940"
     CDS             3873..4253
                     /gene="sdhD"
                     /locus_tag="VIW27_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY5738451.1"
                     /translation="MADLRSPLSKARGLGSAKAGSGHFWHQRLTSLLLVPLVLWLGFS
                     LASMPVDHATLVAWIGQPLVTAALVLLIAVVFYHAQLGLQVVIEDYVGSHAKRTITLL
                     LSNLVCLLAGVVGVVSVLKIAFGA"
     gene            4257..6044
                     /gene="sdhA"
                     /locus_tag="VIW27_01945"
     CDS             4257..6044
                     /gene="sdhA"
                     /locus_tag="VIW27_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY5738452.1"
                     /translation="MTGEYKLIDHAYDVVIVGAGGAGLRATLGMAAAGLSTACITKVF
                     PTRSHTVAAQGGMSASLGNMGPDNWQWHMYDTIKGSDWLGDQDAIEYMCREAVPAVIE
                     LEHFGVPFSRTEEGKIYQRPFGGMTTNFGEGTAQRTCAAADRTGHAILHTLYQQSLKH
                     KAEFYIEYFAIDLIMEEGVCRGVVAWDLASGELHRFRAHTVVLATGGYGRAYFSATSA
                     HTCTGDGGGMVARAGLPLQDMEFVQFHPTGIFGAGVLITEGARGEGGYLTNSKGERFM
                     ERYAPNAKDLASRDVVSRSMTMEINQGRGVGPDGDHIHLHLEHLGAEVLHERLPGISE
                     SAAIFAGVDVTRDPIPVLPTVHYNMGGIPTNYHGEVVTLEDGDPDAVVPGLMAIGEAA
                     CVSVHGANRLGSNSLLDIVVFGRAAALRAAECITPGSKHKPLADDACDSIIERFDRLR
                     NAAGDTRTASIRTRMQRDMQKHAAVFRTGDSLREGVERLDQVFASFADVGISDRGLIW
                     NTDLVETLELENLLLQAQATIHSALNRTESRGGHAREDYTERDDENWMKHTLSWVDDK
                     GRVEFDYRPVHMYTLSDECKVIPPKKRVY"
     gene            6063..6845
                     /locus_tag="VIW27_01950"
     CDS             6063..6845
                     /locus_tag="VIW27_01950"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY5738453.1"
                     /translation="MAEFTLPANSVVKQGKLYSAPEAAKNVKRFVIYRYDPDSGENPR
                     TDTYEVDIDDCGPMVLDALLKIKDEMDSTLSLRRSCREGICGSCSMNINGGNTLACTQ
                     SIEAIKGDVKIYPLPHQPVIKDLVTDLTNFYAQYASVEPWMKTDTATPPDRERLQSRA
                     DREKLDGLYECILCACCSTSCPSYWWNSDRYLGPAALLNAYRWIVDSRDEATGERLDE
                     LEDPFKLYRCHTIMNCAKVCPKGLNPAKAIAETKKLMVQRKL"
     gene            6861..>7014
                     /locus_tag="VIW27_01955"
     CDS             6861..>7014
                     /locus_tag="VIW27_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY5738454.1"
                     /translation="MSELSRLRWLCRRGMKELDVVMTTYLDEHYENASPDQQRQFGKL
                     LEMADPD"
BASE COUNT         1363 a         2156 c         2206 g         1289 t
ORIGIN      
        1 ggatctacga tatcgaactc gaacaggtca ccgtcaagcc gatggacgac ggcctcgcca
       61 acgcgccgat catgatcctg atgaagcagt ccaatatcga cgagcagttc gaaatcagcg
      121 aggtcggtca gcgcaagtat ctctactgga tcgagctcaa gccgcgttcc cgcgaactcg
      181 agtacacgga tatctacatc ggcctcgagg atggccgcgt caaggcgatg gagttgcagg
      241 accgcttcgg tcagagcacg cagatcgtgt tcgacaacct gcgcgtcgac gtggtgcatg
      301 atccggccac tttcaggttc gagccgccgc ccggggtgga tgtctatggc gtgggtggct
      361 gagtcacggc cggaaagtca tccccgcgca agcggggatc ctgcaaccga cgcacgcttt
      421 ttatcgaggc cgagatgaga ttcccgcgtg cgcgggaatg acccgggaaa ggaaatgaac
      481 gatttattcg cggcggaaaa tgcattcaaa ccgctcgcgg accgaatgcg cccgcggcgc
      541 ctcgacgatg tcttcggtca ggcgcacttg ctggcaccgg acaagccgtt gcgccaggcg
      601 atcgaggccg gcaagctgca ctcgatgatc ttctggggcc cgccgggcac cggcaagacg
      661 accatcgcgc ggctgatcgc cgagtactcc gaggcccgtt tcatcaccct gtcagcggta
      721 ctggcgggcg tcaaggacat tcgtgacgcg attgcccagg cgcgccagtt ccaggggctg
      781 acagtgctgt tcatcgacga ggtgcaccgc ttcaacaagg cgcaacagga cgccttcctg
      841 ccgcacatcg aagacggcac gatcctgttc gtcggtgcga ccaccgagaa tccgtctttc
      901 gagctgaaca atgcgctgct gtcgcgcacg cgcacctacg tgctgaaacc gatttcggca
      961 accgatatcg agaacgtggt gcgacgcgcg ctagccgacg acgccagcgg tctcggcgga
     1021 ctcggcctcg agatcgacga cgacgcgctg caaatgctgg cccgcgccgc cgatggcgat
     1081 gcgcggcggg cgctgaccta tctcgaaatc gtcagcgacc tgatcgataa agggcgcatc
     1141 gacgccgcgc tggtcgccga aatcgcacaa caggggccgc gccgtttcga caagggcggc
     1201 gatgtctttt acgaacagat ttcggcgttg cacaagtcgg tgcgcggctc cgatcccgac
     1261 gccgcgctgt actggttttg ccgcatgatc gatggcggtt gcgacccggt ctacatcgcg
     1321 cgccgggtgg tgcgcatggc gatcgaggat atcggtctcg ccgatccgcg cggcctcgaa
     1381 ctgtgcctca acggctggca gactttcgag cggcttggca gccccgaggg tgagttggcg
     1441 attgcgcagg gcatcgttta cctcgcctgc gcggccaaga gcaatgcggt atacgcggcc
     1501 tacaagcagg ccatggacga tgcgcgcaat tcgggcagtc tcgaggtgcc gctgcacctg
     1561 cgcaacgcgc cgacccggtt catggaagag cagggctatg gtgaaggtta ccgttacgcc
     1621 cacgacgagg atggtgcttt cgccgccggc gaggtctatt tcccggaaca gctcggcgcc
     1681 cgcgagtatt accatccggt cgaccggggg cttgaaatcc gtatcgcgga aaaactcgaa
     1741 tcactgcgca ggcgcaactc ggagagcaaa ccatgaacct gccgtatgcc gaggcggcgg
     1801 aacagaacaa gcacgtcatt taccaggcga tcagcggcta tttagccggt gatgccctcg
     1861 agatcggcag tggtaccggc cagcacgccg tttatttcgc cggccgctcg ccggcggtca
     1921 gctggcaaac ctccgacctc ggcgcggcgc tacccggcct cgaggcgcgc attcgcagca
     1981 gcggtctcga caacctgccg ccgccgatcg aactcgacgt gaccggcgcc tggccgccgc
     2041 gcaagttcgc cctggtttac accgccaaca gcctgcatat catgagcagt gcgatggtcg
     2101 aggcctgctt cgccggtgtc ggcgactgcc tgcaatccgg cggggtattc gcggcctacg
     2161 gtccgttcaa ttatcagggt gaatatacct cgccgagcaa tgccggcttc gaccgcatgc
     2221 tgcgctcgcg cgatccgtcg agcggtatca aggattatgt ctggctcacc gagctcgccg
     2281 tagctgcgcg gctggagtta ctcgacgaca tcgaaatgcc ggtaaataac cgcacgttgg
     2341 tctggcaaaa gcgaacttaa tggctcatag ttggactaat ttccgtgctt tagcgaccat
     2401 gaatcaatga acaaaacagt tatcctttcc gccgcggccg gcattctgct gctgatcggc
     2461 gcttccctgc aggcccagga accggcggcg gacgaccagc tgacccgcct gctcaagctg
     2521 cgcctcggca gtgagcagat cgcgccgccc gtcgaaaccg aaatcgacgg cgtttacatg
     2581 acccgcttcg gtaatcgctt tgcctacctg ctggaaggcg ggcgttacat attcgttggc
     2641 gacctggtgg acctcgagca gggccgcaat ctcaccgagt tgagccgccg cgagatggtc
     2701 gtcgacgagc tcgccgcctt cggcagcgaa aagcgcgtca tcttcccggc cgtcgacgaa
     2761 gagaaagcgg tgttgagcgt gtttaccgac accagctgcg gctattgcca gcaattgcac
     2821 cgcgaggtgg agtacctgca gcaggccggt atctcggtgc actacattcc ctttccccgc
     2881 ggcggcaatc gcggcccggg ctaccaggac ctgaagcagg tctggtgttc gcaagatccg
     2941 ctcgaggcga tgagcatcgc caagaaggcg cgcccgggtg acctggatgc agcggagaac
     3001 tgcgaatcgg cgcagtatgt ggacgagggc taccagctcg gcaaccgcat aggcgtgagc
     3061 ggcacgccat cgctttatac ggccgacgga actcacttca acggttatgt gccttaccgg
     3121 gagctcatcc agcgcctgct gatgagtccg tagttttttc aatacaacgc atggaaaatt
     3181 ttgctcggcg gacccgcaaa cggcgtttag tgggtgtcgc ggcggtttac tgtggtaaaa
     3241 tccgcgccca atgaaaaaga atcctgaagc tgcaccgaag acggaaacaa aagtcgaggt
     3301 cagtgtcgac gatgcgcggc tcgacgggcc cggcggcgat aacgacgtcc cggccgagta
     3361 cggcggtccc cgcggcaagg agccgacccg ttacggtgac tgggaaagaa acggccgctg
     3421 tatcgatttc tgatcgatcc ggtaatttag ctgcgacgta tacaacaact cgggaagggc
     3481 atcgatgtcg ttacgcaagc atcctacatc gccgcatctg caaatctacc ggttgccgct
     3541 gaccgcgctg ttgtcgatca cgcaccgcat caccggcgtg ttgctggctg tcggctgcgt
     3601 gctgctgatc ggcattctcg ccgcggcggc cgacggtgcg gccacctatg aggcgatcgt
     3661 gccgcacctg caaagctggt acggtcaact gttgctgatc ggcctggttt tctctctcta
     3721 cctgcatttc tgtaatggcg tgcgccacct ggtctgggac gcgggctacg gtttcgaact
     3781 ggatacggtc gatttgaccg ccaagctggc gatcgcgctg gcgatcgcat tgactgccgc
     3841 aacctggctg gttgcaggga gctggtaacg gcatggctga tctcagatca ccgttgtcga
     3901 aagcccgcgg cctgggctcg gcgaaagcgg gtagcggcca cttctggcat cagcgcctga
     3961 cctcgctgtt gctggtgccg ctggtgctgt ggctgggctt cagtctggcg tcgatgccag
     4021 tcgaccatgc cacgctggtg gcgtggatcg gacagccgct ggtgacggcc gccctggtgc
     4081 tgctgatcgc ggtggtcttc taccatgcgc agctgggcct gcaggtcgtc atcgaggatt
     4141 acgtcggctc gcatgccaag cgcacgataa ccttgttgct atcgaacctg gtctgcctgc
     4201 tggcgggcgt ggtcggcgtg gtctcggtac tcaagatcgc gttcggagcc tgatcgatga
     4261 ccggtgaata caaactgatc gatcatgcct acgacgtggt catcgtcggt gccggcggcg
     4321 ccggtctgcg ggcaacgctg ggcatggcgg cggccgggtt gtcgacggcc tgcatcacca
     4381 aggtgttccc gacccgcagc catacggtgg cggcgcaggg cggcatgagc gcgtcgctag
     4441 gtaacatggg tcccgataac tggcaatggc acatgtacga cacgatcaag ggatccgact
     4501 ggctcggcga ccaggacgcg atcgaataca tgtgccgcga agcagtgccg gcggtgatcg
     4561 agctcgagca tttcggagtg ccattctcgc gtaccgagga aggcaagatt taccagcggc
     4621 cgttcggcgg catgaccacc aatttcggcg agggcaccgc gcagcgcacc tgcgccgcgg
     4681 ccgaccgtac cgggcacgcg atcctgcaca ccctctacca gcaatcgctc aagcacaagg
     4741 ccgagtttta tatcgagtat ttcgccatcg acctgattat ggaagaaggc gtctgtcgcg
     4801 gcgtggtcgc ctgggatctc gccagcggcg aattgcaccg ctttcgcgct cacacggtgg
     4861 tgctcgccac cggcggttat ggccgtgcct acttttcggc gacttcggcg catacctgta
     4921 ccggcgatgg tggcggcatg gtcgcgcgtg ccggtctgcc gctgcaggac atggagttcg
     4981 tgcaattcca tcccaccggt atcttcggcg ccggcgtgct gattaccgag ggcgcgcgcg
     5041 gtgagggcgg ctatctgacg aattccaaag gtgaacgttt catggagcgt tacgcgccca
     5101 acgccaagga cctggcctcg cgcgacgtgg tcagccgttc gatgacgatg gaaatcaacc
     5161 agggccgcgg cgtaggcccc gatggcgatc atatccacct gcacctcgag catctgggcg
     5221 ccgaggtgct gcacgagcgc ctgccgggca tctccgagtc cgccgcgatc tttgccgggg
     5281 tcgatgtaac ccgtgacccg ataccggtgc tgccgaccgt gcactacaac atgggcggca
     5341 taccgactaa ttaccacggc gaggtcgtaa ccctcgagga cggcgacccg gatgccgtgg
     5401 tgccggggct gatggcgatc ggcgaggccg cctgtgtctc ggtgcacggc gccaatcgac
     5461 tgggttccaa ttcgctgctc gatatcgtcg tcttcggccg cgccgccgcg ttgcgcgccg
     5521 ccgagtgcat caccccgggc agcaagcaca agccgctcgc cgacgatgcc tgcgattcta
     5581 tcatcgagcg tttcgaccgc ctgcgtaacg ctgccggcga cacgcgcacg gcgagcattc
     5641 gcactcgcat gcagcgcgac atgcaaaaac atgcggcggt gttccgcacc ggcgattcgc
     5701 tgcgcgaagg cgtcgaacgg ctagaccagg tattcgccag ttttgccgac gtcggcatca
     5761 gcgaccgcgg cctgatctgg aataccgacc tggtggagac gctggagcta gaaaacctgc
     5821 tgctgcaggc gcaggcaacg atacacagcg cactcaaccg taccgaaagc cgcggtgggc
     5881 acgcgcgcga agactatacc gagcgcgacg acgaaaactg gatgaaacac accctgtcct
     5941 gggtcgacga caagggcagg gtcgagttcg attatcgccc cgtgcacatg tacacactga
     6001 gtgacgaatg caaggtaata ccgccgaaga agcgggttta ttgaggtctg gaagcgagtt
     6061 agatggcaga attcacgtta cccgccaact ccgtggtcaa acagggcaag ctgtatagcg
     6121 cgcccgaagc tgcgaaaaac gtcaagcgct tcgtgattta ccgctacgat ccggacagcg
     6181 gcgaaaaccc gcgcaccgat acctacgagg tagatatcga cgactgcggg ccgatggtgc
     6241 tcgacgcgct attgaagatc aaggacgaaa tggattcgac gctgtcgctg cgccgttcct
     6301 gccgcgaggg gatttgcggt tcgtgctcga tgaacatcaa cggtggcaat accctggcct
     6361 gtacgcagtc gatcgaagcg atcaagggcg acgtcaagat atatccgctg ccgcaccagc
     6421 cggtgatcaa ggacctggtg accgacctga ccaacttcta tgcgcagtac gcttcagtcg
     6481 agccgtggat gaagaccgat accgcaacgc cgccggatcg cgagcgcctg cagtcgcgcg
     6541 cagaccgcga aaaactcgac ggcctctacg aatgcattct ctgcgcctgc tgctcgacca
     6601 gttgcccgag ttactggtgg aacagcgatc gctatctcgg ccccgccgcg ctgctcaacg
     6661 cctatcgctg gattgtcgac agccgcgacg aggcgaccgg cgagcgactc gacgagctcg
     6721 aggacccgtt caagctctac cgttgccata cgatcatgaa ttgtgccaag gtttgtccca
     6781 aggggctgaa tccggccaag gcgatcgccg aaaccaagaa actgatggtg cagcgcaagc
     6841 tgtagcaaac ggccgacgcg gtgtccgagc tgtctcgcct gcgctggttg tgccgccgtg
     6901 gcatgaaaga gctcgacgtg gtgatgacca cttacctcga cgagcactac gagaacgcgt
     6961 cgcctgatca gcagcggcag ttcggcaagc tactcgagat ggccgacccc gacc
//
LOCUS       DATCZV010000087         9223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_218034, whole genome shotgun sequence.
ACCESSION   DATCZV010000087 DATCZV010000000
VERSION     DATCZV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218034"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VIW27_01960"
     CDS             complement(<1..384)
                     /locus_tag="VIW27_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HEY5738455.1"
                     /translation="MSSQSGNGNRGGPWGAANSSPPDLEQLLDSGRRWLRHFVPGGGA
                     SSVVMFAVIAALLVGAWTAYYTVPSDSVAVVQRFGKYVKNVQPGLHFKFPLGIDTAII
                     VPVKRQLKQEFGFTTPGASDPYQSPV"
     gene            complement(724..1047)
                     /locus_tag="VIW27_01965"
     CDS             complement(724..1047)
                     /locus_tag="VIW27_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738456.1"
                     /translation="MYARQVSMELNPNSLTNFTAKLESEIIPLLRKQAGFMDELTFVE
                     PDGKRAFAVSLWDSKQNAEAYSQASYTDVTKMLSGMIQGTPQVKTFDVVHSTSHKIGA
                     KQKAA"
     gene            1219..2085
                     /locus_tag="VIW27_01970"
     CDS             1219..2085
                     /locus_tag="VIW27_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5738457.1"
                     /translation="MDLGLAIKKRLQELGLEQKDLATAAEVTDSYISQLLNRRKLPPA
                     PDRTDIYEKMDKFLRISKGELARLARAQRLEEYQRGYAEPEVALLKETRELILRKCEP
                     GNQRQIRSIFEKQSFGELERLVTQKLLEVARQVTRSELDNEAWLREVAQLCNKSYQQL
                     RVIMLEFLDTDIFNVSNEDCVSFLDPLIRQWDIDLSTFDMEISLNDRLVPGEPKKFAF
                     VEQGRDGPRKKEPGLVEFLQDPVLSRDITGEELEFLTRLRFGATRRPNRLYYYRTLQN
                     LRDPLHFGAVKE"
     gene            complement(2426..3118)
                     /locus_tag="VIW27_01975"
     CDS             complement(2426..3118)
                     /locus_tag="VIW27_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY5738458.1"
                     /translation="MARILLLLGLAAGLFFLFRWLSHQPPRVHAQLSMVLLSLGLLVL
                     VLSGRAHWLLAVVAALLPFLRGWLSGRGAPRPKAEPAGGQKSTVHSRYLRMTLDHDSG
                     DLDGEVLSGRFAGKSLDQLDLDTLLQLLDECRDDDESLALLQAYLEREHGDSWQQAAG
                     AGTGRQAGASAPGVMSRAEAFEVLGLSADASEAEIIDAHRRLMQKLHPDRGGSAYLAA
                     TINLAKETLLSK"
     gene            complement(3122..3835)
                     /locus_tag="VIW27_01980"
     CDS             complement(3122..3835)
                     /locus_tag="VIW27_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5738459.1"
                     /translation="MRDADKDLPQKSSKTAIDAFVDRLRSTPARKGRGQRGRLMFAMD
                     ATASREPTWDRACRIQGDMFSETAALGGLDIQLCYYRGYGEFEASPWLSSAQALLERM
                     TGVSCRGGQTQIEKVLRQAIDQHKSKRIQSLVFVGDCMEEDVDQLCQLAGQLGVLRLP
                     VFLFHEGNDVSARRAFEEIARLTRGAYCPFDASSAGQLRDLLSAVAVYAAGGQQALQD
                     FTRDRSPALRQLTRQLEKR"
     gene            complement(3962..5026)
                     /gene="sohB"
                     /locus_tag="VIW27_01985"
     CDS             complement(3962..5026)
                     /gene="sohB"
                     /locus_tag="VIW27_01985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEY5738460.1"
                     /translation="MTEFFFEYGLFAAKLVTFVIIVVAAVIVAFGITRRVRRSMKGYI
                     ETTRVNDEIQAMHDAVDAGVSDPESFKLHIKQRRREQKLERKARKTALKQAHGGDADA
                     KPRLFVLDFEGDLRASAVSRLRHEITAVLSMARDGDEVAVRLESRGGMVHAYGLAASQ
                     MQRIRDRGIALTVCVDKVAASGGYMMACVADRILAAPFAILGSIGVVAQLPNFNRWLK
                     KNDIDFEMITAGEYKRTLTIFGENTDKGREKFVEEIEDVHLMFKEFVAEHRPGLDIEG
                     AATGETWFGQRALKRGLVDELVTSDDYITGACDRADVFEVRYVERKTLSERFGVVLQN
                     SADRLLLKWWERGTQARFFS"
     gene            5333..6262
                     /locus_tag="VIW27_01990"
     CDS             5333..6262
                     /locus_tag="VIW27_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5738461.1"
                     /translation="MPDQSTLPSPHAVDSRRGYAMLLMAIGSTAISFGGLVQRSLEVA
                     DPWQINIYRSLGLIVAIGVIVALQNRGRFFHTLLSVGRFGILAGVLLAGAGISFIQAF
                     THTTIANALFIFGAIPFFAALLARLFLGERLRRSTLVTMLFAGGGLALMVFNGINIGS
                     GFGNLMALATALCFAGYAVIVRYRRRVEMLPTLLIASALIIVVSLPASGGDLALPWRD
                     ILLCLFWGGCLSGFVNWTFIIASRHLAAAEVTLLMLIEFALGPVWVWWFVGETPSGWT
                     LIGGSVIIAAVAARAILELINKPRPEQTPGQPV"
     gene            6335..7030
                     /locus_tag="VIW27_01995"
     CDS             6335..7030
                     /locus_tag="VIW27_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HEY5738462.1"
                     /translation="MESWITIDSTLIPGSETELTLSRRNDDFAIRIAGVPGDLMNSRR
                     HHSEDALAELGCDHLRSKPSARILVGGLGMGFTLAAALEIVGESAEVVVAELVPAVVE
                     WNRGPLGQCAGRPLADSRSRVHLGDVAELIKQPANLFDAILLDIDNGPEAMTHADNEW
                     LYSPAGLRSIHAKLRPGGIVTTWSARADHQFAKRLQKAGFEVQSRTVRARPGKGSRHT
                     IFVARKPKAHNKR"
     gene            7092..7646
                     /locus_tag="VIW27_02000"
     CDS             7092..7646
                     /locus_tag="VIW27_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009021410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEY5738463.1"
                     /translation="MTEPIDKDAITAAVRRWVETVVVDLDLCPFAGRELHRERIRFAV
                     TPARTAAELLASLRTELEMLDTHPDIETTLLVHPRVLQDFDAYNDFLDTADELLDRLR
                     MVGTFQIASFHPHYRFAGTAAEDVENYTNRSPYPILHLLREASLQRAIESHPDTERIP
                     RANIALMKRLGAGRLKQLWQACFG"
     gene            complement(7705..8826)
                     /locus_tag="VIW27_02005"
     CDS             complement(7705..8826)
                     /locus_tag="VIW27_02005"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY5738464.1"
                     /translation="MMDRFLLAHGAGQPGDRLLDDCLNQLGDIPPEANFGFLYLSDAL
                     ADMAESLIERLERSTGIAAWVGTVGIGIIGGEMEYYDEPAMAIMLAGFNEADYRLLPK
                     LRDDNASLSAGLLDWCGANDFNVGLLHADPETVNLQPLLARLDEEIPAAFLIGGISSS
                     RGYHAQFAGQVFTGGLSGALFSQAVPIMTNLTQGCSPIGPRHVVSQCERNILHSLDDR
                     PALDVLTEDVGEVIAQDWQQAAGYIFAGIVNKNSDQDDYSIRQLIGIDPDAKLVAIGD
                     FLEHNDEIIFCKRDGGTALEDMERMLRELKKRCPSTIKGGVYVSCLGRGRYQFGDNSE
                     EIRFIHAVLGDFPLVGFFANGEIHKSALYGFTGVLTLFV"
BASE COUNT         1802 a         2903 c         2764 g         1754 t
ORIGIN      
        1 gacagggctc tggtaagggt ccgaggctcc cggggtggtg aagccgaact cctgtttcaa
       61 ctggcgcttg accggaacga tgatggccgt gtctatgccg aggggaaact tgaagtgcag
      121 tccgggctgt acgttcttga catacttgcc gaagcgctgg acgaccgcga ccgaatcgct
      181 cggcaccgtg taatacgcgg tccaggcgcc gaccagcagc gccgcgatga ccgcgaacat
      241 gacgacagag ctcgctccgc cgcctggaac gaagtgcctg agccaccgcc gcccactgtc
      301 gagcaattgc tcgaggtcgg gcggggaact gttggcggcg ccccatgggc cgcccctgtt
      361 gccgttaccg gattgcgaag acatgcgggt cgttttctcc ttgctgtacg ccgcgaggcg
      421 ttggtttacc tggcacgaaa atacatgcct tcgtcttcgg ctggctgcaa agcaagccgg
      481 gcggctctgc ggagacgccg tttcttttct ggtgtcagtg gcaggcgagc cttcgcggaa
      541 accgatttcg gcaagtgcaa agtctcacgc cgaggcgtct gccccgttgc cggcgatgcg
      601 ttggccgaca caggccgacg cgaattcgtc tggtaaggca ttttcaagag tccggtccgc
      661 aagcgggcag gtaaaaaaac ggcaagcgaa tgactccgcc tgccgtaaaa acacccgcta
      721 gaattaggcc gctttttgct tcgcgccgat cttgtgggag gtggagtgga cgacgtcgaa
      781 ggtttttacc tgcggcgttc cctggatcat cccagataac atcttggtta catcggtata
      841 cgaggcctgg ctgtaagcct cggcattttg cttgctgtcc cagaggctca ccgcgaaggc
      901 tcttttgcca tccggctcga caaacgtgag ttcatccatg aaacctgcct gcttgcgcag
      961 cagagggatg atttccgatt ccagcttggc ggtgaaattg gtcagactat tcgggtttaa
     1021 ttccattgat acttgacgtg cgtacatgca tttctccttg cgattggtca gcagggcact
     1081 ccggatggat catttgctta tcgaatccga agcccagttg atccggcaac tataatcggt
     1141 taattaactt gttttgtcaa ccgaaatctg agaatatttc agtcaattaa ctatttttgt
     1201 aaatcaagag agagaacaat ggatcttggc ctggccataa aaaaacgctt gcaggaactg
     1261 gggctcgagc agaaggacct ggccaccgcc gccgaggtga cagattccta catctcccag
     1321 ctgctgaacc gcaggaaatt accgcccgcg ccggatcgaa cagacatcta cgaaaagatg
     1381 gacaagtttt tgcggatctc caaaggcgag ctggcgagac tggcgcgcgc ccagcgactg
     1441 gaagaatatc agcgaggtta cgccgagccg gaagtcgccc tgctgaagga aacccgggaa
     1501 ctgatattgc gcaagtgcga gccgggcaac cagcggcaga tccggtcgat attcgagaag
     1561 cagtcgttcg gcgaactcga gcggctggta acgcaaaagc tgctggaggt cgcccgccag
     1621 gtcacgagat cggaactgga taacgaggcc tggctgcgcg aagttgcgca gctgtgcaac
     1681 aaaagctacc agcagttgcg cgtcatcatg ctggaattcc tggacacgga tattttcaat
     1741 gtctcgaacg aagactgtgt atcctttctc gatccactga ttcgacaatg ggatatcgat
     1801 ctttcgactt tcgatatgga gatatcgctg aacgacaggc tcgtccccgg ggaaccgaag
     1861 aaattcgcct tcgtggagca aggccgcgac ggtcctcgca agaaagaacc ggggctcgtc
     1921 gaatttctgc aagacccggt tctaagccgt gacataaccg gggaggaact cgaattttta
     1981 actcggctca ggttcggggc gacccgccgg cccaatcgac tctattatta ccggaccctg
     2041 caaaacctga gagatccgct gcattttggc gccgtgaaag aatgaccgcc tggtacgcgg
     2101 cagcgcaccg ctagcagcgc aggcggtatt tcggggtcgg tatttcgggt atttcggggt
     2161 cggaccacgc taacctattg atcgatataa caataaccgt caaaagcgcc ctgttattct
     2221 ccttagaccg cccattttcc cgtcaaaagg accgcttaag tcaggtgcac ggtttcctga
     2281 tttcgtttac tcgttaaaat gcgtttttcg gcaccaaaaa tgtcgcttta ggcccaaaaa
     2341 tcggcccgtt ttcgaaaata acctattatc tttcagccac ttaacgtggt ccgaccccgt
     2401 tggcggcccg ttggtgcttg gtcccttact tcgacagcag ggtttccttg gccaggttga
     2461 tcgtcgccgc gaggtaagcg gagccgcccc ggtccgggtg cagcttctgc atcaatcgcc
     2521 ggtgcgcatc gataatctcc gcctcgctcg cgtcagctga caggccgagc acctcgaacg
     2581 cctcggcgcg cgacattacg cccggcgcgc tggcgcccgc ttgccgtccc gtcccggccc
     2641 cggccgcctg ttgccagctg tcgccgtgct cgcgctcgag gtaggcttgc aacagggcga
     2701 gcgattcatc gtcgtcgcgg cattcatcca gcagttgcag cagcgtatcg agatccagct
     2761 gatcgagcga tttgcccgcg aatcgcccgg acagcacctc gccgtcgagg tcgccggaat
     2821 cgtgatccag ggtcatacgc aggtacctgc tgtgcaccgt cgacttttgc ccgccggcgg
     2881 gttcggcttt cggccgcggc gcgccccggc cgctcagcca gccgcgcagg aagggcagca
     2941 acgcggcaac caccgccagc agccagtgcg cgcggccgga gagcaccagc accagcaatc
     3001 ccagcgacag caggaccatg gacaattgcg cgtgaacccg cggcggctgg tgcgacaacc
     3061 agcggaacag gaagaacaat ccggccgcga ggccgagcag cagcaaaatc cgcgccatgg
     3121 ctcagcgctt ttccagctgc cgggtcagtt ggcgcagtgc cgggctgcga tcgcgggtga
     3181 aatcctgcaa cgcctgttgg ccgccggcgg catacaccgc gaccgcgctc agcagatcgc
     3241 gcagctgccc ggcgctggag gcgtcgaacg ggcagtaagc gccgcgcgtg aggcgcgcga
     3301 tctcctcgaa ggcgcgccgc gcgctcacat cgttgccctc gtggaacagg aacaccggca
     3361 gcctgagcac gcccagctgg cctgcgagct ggcacagctg atccacgtct tcctccatgc
     3421 agtcgccgac gaacaccagc gactggatgc gcttgctctt gtgctgatcg atggcctggc
     3481 gcagaacttt ttcgatctgc gtctgaccgc cccggcagga cacgccggtc atgcgctcga
     3541 gcagggcctg cgcggaggac agccacgggc tcgcctcgaa ttcgccgtag ccgcggtagt
     3601 agcacagttg aatatcgaga ccgccgagcg cggcggtttc gctgaacatg tcgccctgga
     3661 tgcggcaggc gcggtcccag gtaggctcgc ggctggcggt ggcatccatc gcgaacatca
     3721 accgcccgcg ctgcccgcgg cccttgcgcg ccggtgtcga gcgcagccga tcgacgaacg
     3781 cgtcgatcgc ggtcttgctc gatttttgcg ggagatcttt atcagcgtcc ctcatgccat
     3841 tcgggttcga ggcgtcaatg ctggcacagt atacccgttt tccgcgcgcc gattcgcacc
     3901 agggcgcgag cccgtgcgat tgcacgcagg cgccgcgacg ggacaggcaa cccgggcggc
     3961 atcaggaaaa aaaacgcgct tgcgttcccc tttcccacca cttgagcagc agccggtcgg
     4021 ccgaattttg cagcaccacg ccgaaacgct ccgacagcgt tttccgctcc acgtaacgga
     4081 cctcgaagac gtccgccctg tcgcaggcgc cggtgatgta gtcatcgctg gtgaccagtt
     4141 catcgaccag gccgcgttta agcgcgcgct gcccgaacca ggtctcaccg gtggcggcgc
     4201 cttcgatgtc tagcccgggc cggtgctcgg cgacgaattc cttgaacatc aggtgcacat
     4261 cctcgatttc ctcgacgaac ttctcgcgcc ccttgtcggt gttctcgccg aatatcgtca
     4321 gcgtgcgctt gtattcgccc gcggtgatca tttcgaaatc gatgtcgttc tttttcagcc
     4381 agcgattgaa attcggcagc tgcgcgacca cgccgatcga gccgagtatc gcgaaaggcg
     4441 cggcgaggat ccggtccgcc acgcaggcca tcatgtagcc gccgctggcg gcgactttgt
     4501 cgacgcaaac ggtcagcgcg atgccacgat ccctgatgcg ctgcatctgc gaggcggcga
     4561 gcccgtaggc atgcaccatg ccgccacggc tctccaggcg cacggccacc tcgtcgccat
     4621 cgcgcgccat cgacagcacc gcggtaatct cgtggcgcag gcgggatacc gcggacgccc
     4681 tgaggtcacc ctcgaaatcg agcacgaaca gccgcggttt cgcgtccgcg tcgccgccgt
     4741 gtgcctgctt gagcgctgtc ttgcgtgcct tgcgttcgag cttttgctca cgccgtcttt
     4801 gctttatgtg tagcttgaaa ctttccgggt cggagacccc cgcgtcgacc gcgtcgtgca
     4861 tcgcctggat ctcgtcgttg acccgggtgg tctcgatata gcccttcatc gaacggcgca
     4921 cgcgccgggt gatgccgaag gccacgatga cggcggccac gacgatgatg acgaaggtta
     4981 ccagcttggc ggcaaacaaa ccgtactcga aaaagaattc agtcacgatg atctcctgta
     5041 aggcgaatcg ggcaggcgca ccgcagccac gcgcagcggc agcgcggccg cgagccggcg
     5101 gcatgtcttc tgcatatggt gactgttgct aaaattacca gcccgcaggc attggcgtgc
     5161 aacgatgaaa ccgggcgaag atcgagtgcc cattcaccgt gcatggcccc gtgagccggt
     5221 gagcccacga tcgagcccgc aataaaccgg ggcaaggaca ggcgggcata tattgctgga
     5281 caaaacccct ggcctggcgg aatcttgccc ggctaattta ccggggccgc gcatgccgga
     5341 tcagtccacc ctaccctcgc cacacgcagt cgacagccgc cgcggctacg cgatgctgct
     5401 gatggcgatc ggctcgaccg cgatcagttt cggcgggctg gtgcagcgca gcctcgaagt
     5461 cgccgacccg tggcaaatca acatctatcg ctcgctcggc ctgatcgtcg cgatcggggt
     5521 catcgtcgcg ctgcaaaacc gcggccggtt tttccacacc ctgctgtccg tgggccgttt
     5581 cgggatcctc gccggcgtgc tgctggccgg cgccggcatc agtttcatcc aggcgttcac
     5641 gcacaccacc atcgccaacg cgctgttcat cttcggcgcc attccgtttt tcgcagcgct
     5701 actggcgcgc ctgttcctcg gtgagagatt acgccgctcg accctggtga cgatgctgtt
     5761 cgccggcggc ggactcgcgc tgatggtctt caacggcatc aacatcggct cgggcttcgg
     5821 caacctgatg gcgcttgcga ccgcgctgtg ctttgccggc tacgcggtca tcgtgcgcta
     5881 ccggcgccgc gtcgaaatgc tgccgacgct gctgatcgcc tcggcgctga tcatcgtcgt
     5941 ctcgctgccc gcaagcggtg gcgacctcgc gctgccgtgg agagatatcc tgctgtgcct
     6001 gttctggggc ggctgcctgt cgggtttcgt caactggacc ttcatcatcg cgtcgcgcca
     6061 tctcgccgcc gccgaggtga ccttgctgat gttgatcgaa ttcgcgctgg gcccggtctg
     6121 ggtgtggtgg ttcgtcggcg aaactccgag cggctggaca ctgatcggcg gcagcgtgat
     6181 catcgccgcg gtggcggcgc gcgccatcct cgaactgatc aacaaaccgc gccccgagca
     6241 aacgccgggg cagccggtct gaaattgcgg aattgctccg cgccgataca tgatatcgtg
     6301 cgcgcgtgaa acgccggcaa gcattgcacg tgacatggaa tcctggataa ccatcgacag
     6361 caccctcatt cccggcagcg agaccgagct gaccctgtcg cggcgcaacg acgatttcgc
     6421 gattcgtatt gccggggtgc ccggcgacct gatgaacagc cgcaggcatc actccgaaga
     6481 tgcgctggcg gaactcggct gtgatcatct gcgctcgaaa ccatccgcac gaatactcgt
     6541 cggcggactg ggcatgggct tcacgctcgc ggcagcgctc gaaatcgtcg gcgagtcggc
     6601 cgaggtggtc gtggccgaac tggtgccggc agtcgtggaa tggaaccgcg gccccctcgg
     6661 gcaatgcgcg ggacgaccgc tcgcggatag ccgcagccgc gttcacctcg gcgacgtggc
     6721 cgagctgatc aagcagccag cgaatctatt cgatgcgatc ctgctcgata tcgataacgg
     6781 ccccgaggcg atgacgcatg ccgataacga atggctctac tcaccggcgg gtttgcgcag
     6841 cattcatgca aaacttcgac ccggcggcat cgttacgacc tggtcggcca gggccgatca
     6901 tcaattcgcc aaacggctcc aaaaagccgg tttcgaagtc cagtcgcgca cggtaagggc
     6961 tcgtccgggc aaaggcagcc gccatacgat attcgtcgcc cgcaagccga aagcgcataa
     7021 taagcgctga aaagaaattg aagatcaaca gtttcgatga accgtaacgc agcagagtcc
     7081 gaacgtcgcc ggtgaccgag ccgatcgata aagacgcgat caccgcagcc gtgcgccgct
     7141 gggtcgaaac cgtcgtggtg gacctcgatc tctgcccgtt tgccgggcgg gaactgcatc
     7201 gtgagcgcat ccgcttcgcg gtgaccccgg caaggacggc ggcagagctg ctggcgagtc
     7261 tgcgcaccga gctcgaaatg ctcgacactc accccgacat cgaaaccacc ctgctcgttc
     7321 acccccgggt gctgcaggat ttcgatgcct ataacgattt cctcgacacc gccgacgaat
     7381 tgctggacag gctgcggatg gtcggcacct tccagatcgc gagctttcac ccgcattacc
     7441 ggtttgccgg caccgcggcc gaagacgtcg aaaactacac caaccggtcg ccttacccga
     7501 tcctgcatct gctgcgggaa gccagtctcc agcgcgccat cgaaagccat cccgataccg
     7561 aacggatacc gcgcgccaac atcgcgctga tgaagcgcct cggggccggg cggctaaaac
     7621 agctctggca ggcctgtttc ggttgacgcg attgcgggga ccgaaaacct gactgcccgg
     7681 ccatcggccc gcgcgcgggt cgcgttaaac gaacagggtc agcacgccgg taaaaccata
     7741 cagcgcactc ttatggatct cgccgttggc gaaaaagccg accagcggga aatcgcccag
     7801 cacggcatgg atgaatcgaa tctcctccga attatcgccg aactggtacc tgccacggcc
     7861 caggcaggag acataaacac cgcccttgat ggtcgatgga cagcgcttct tcagttcccg
     7921 cagcatgcgc tccatgtctt cgagcgccgt gccgccatcg cgtttgcaga aaataatctc
     7981 gtcgttgtgc tcgaggaaat caccgatagc gacgagcttc gcatccggat cgataccgat
     8041 gagctggcgg atcgagtaat cgtcctggtc cgagttctta ttgacgatac cggcgaagat
     8101 gtagccggcg gcctgctgcc agtcctgcgc gatgacctcg ccgacgtctt cggtcagcac
     8161 gtcaagcgcg ggccgatcgt cgaggctgtg caggatattg cgctcgcact ggctgacgac
     8221 atggcgcggc ccgatcgggc tgcagccctg ggtgagattg gtcatgatcg gcaccgcctg
     8281 tgaaaacagc gcaccgctga gtccgccggt aaagacctgc ccggcgaatt gcgcgtgata
     8341 cccgcgcgac gagctgatcc cgccgatcaa aaacgcggcc ggaatctcct cgtccaggcg
     8401 tgccagcaag ggttgcaggt ttaccgtttc cggatccgca tgcaacaggc caacattgaa
     8461 atcgttggcg ccgcaccagt ccaagagccc ggcggagaga gaggcattat catcgcgcag
     8521 cttcggcagc agccgataat cggcctcgtt gaacccggcg agcatgatcg ccatcgccgg
     8581 ctcgtcgtaa tactccatct caccgccgat gatgccgatg ccgacggtgc caacccaggc
     8641 cgcgatgccg gtcgaccgct cgagccgctc gatcagggac tcggccatgt cggccaatgc
     8701 gtcactcaag tacaaaaagc cgaaattggc ctcgggcgga atatcgccca gttgattgag
     8761 gcaatcatcg agaagccggt cgccgggctg gccggcgccg tgagcaagca ggaatcgatc
     8821 catcatgacg ggccagttta cttgaaagtg gcccgatggg cagtattccc aactcggcat
     8881 tagcgggtgc gggtggttgc cttcaagcaa ggcggcttaa ccgatggctg tgcgtccact
     8941 taaagattgg gaaatactgt tcagatcaac cgcgccgcct ctcatcacca gaacccgata
     9001 cgtcgggccg cgaaatccat ccacaagtca tttaatcccc ctcatcgcaa atggatgccg
     9061 cagcccgacg tatcgggtcg ggcccggcgt gacaacttca gcctcctgta gtcattcccg
     9121 cgcaagcggg aatcttcgca cgagcctcct gtagtcattc ccgcgcaagc gggaatcttg
     9181 gaacgagccg ccattacaag atccccgctt gcgcggggat gac
//
LOCUS       DATCZV010000088         4803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_641117, whole genome shotgun sequence.
ACCESSION   DATCZV010000088 DATCZV010000000
VERSION     DATCZV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641117"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VIW27_02010"
     CDS             complement(<1..262)
                     /locus_tag="VIW27_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5738465.1"
                     /translation="MKLRRVRGFTLVEVLVAAVIMFTVLATATLSLRTALHASERAAR
                     TAELLAPLPWITPTIREDLRANPAAERVGAGTMFGVEYRFHAV"
     gene            complement(259..654)
                     /locus_tag="VIW27_02015"
     CDS             complement(259..654)
                     /locus_tag="VIW27_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY5738466.1"
                     /translation="MLRKARGFTLIEILLVVLLASILVTLVAPLGIEQVEKARAQSEW
                     LTLDREIDRLSLDAFLRSDFVTVYAAGKRLAWESRGGQPGVIEFEQLFFSPEQRITIN
                     PNGIADRLAIEVLQRDRRRSLSILPEVRE"
     gene            complement(654..1868)
                     /locus_tag="VIW27_02020"
     CDS             complement(654..1868)
                     /locus_tag="VIW27_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5738467.1"
                     /translation="MQQRFHYSGFDAAGSSVNGTVEAASLPQARAELRERDILVSEIR
                     PAAETQDWLDSLGLGESRVGLAQLEIITAELALLLENGVRIDRGLEILRRGSSNPATH
                     RLLDGLLQSLKQGNQLSAAVAEWPQVFDPLYVNLLSLGEASGRLAAVFRRLADDLGFR
                     RDLRRKVVTAVTYPLVILTVCVISLLFIFNFVVPNLASLFADYPELPWYTQALMGVSD
                     FMQRYQLLLGGIIGVVAVIFFYLPRWPAMQMAWHEFAANAPGLRDGISMVERIRLTGG
                     MAMMLEAGVPVDRALQLASSSVKSAPIRRELLVALGKLRQGEGISMVLRQTRLFPDFY
                     ASLLEVGEESGALAPVFAEIAGRSRQAFTEWTQRFTSLLEPALILFMGVVVGAVVVVI
                     MLSITSITGAAF"
     gene            complement(1869..3524)
                     /locus_tag="VIW27_02025"
     CDS             complement(1869..3524)
                     /locus_tag="VIW27_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5738468.1"
                     /translation="MSAVLDVFSEHGVPAADLEKARSYQAKHGGRLEQILVNMGSLPA
                     EDLPAIYSKWFGAPVLSEADWRDWQLPEGSDALPLEFLLERGWLPWERDADSWVFASC
                     SPFDLEVNAWFAEHALRPALRLLSADDFETLRSRLGIGKSDSQLDGLSLVEEDRLREM
                     ASEAPTVNLLNSLIAKALRRGASDMHVEPHAGRARVRYRVDGVLHEIESIPTAMMLPL
                     ITRLKILAAMDIAERRRPQDGKIELRIGGEEVDIRVSALPLHEGGESAVLRFLRKATL
                     VYDMSLLGLSPDIEARILEDLEVTSGVILLTGPTGSGKTTSLYTFMSRINSPEVKIIT
                     LEDPVEYQLDGINQVQVQPEIGYDFARGLRSIVRQDPDVIMVGEIRDRETASVAMQSA
                     LTGHLVFSTVHTSDAPSAFTRLLDLGVEEFLLNAALRSIVAQRLVRRLCKCSTAHPDA
                     AQLMARYQLAELVERSGLAEPDLRQSVGCDDCGHTGYRGRLAIVEYLRCDDAIAAMNK
                     GERFIAEAREHMRSLGGRSLVEDGLLKALCGDTTVEEVFRVAG"
     gene            complement(3521..3946)
                     /gene="gspG"
                     /locus_tag="VIW27_02030"
     CDS             complement(3521..3946)
                     /gene="gspG"
                     /locus_tag="VIW27_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009838145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY5738469.1"
                     /translation="MKSKAIQGIERRQSGGFTLIEILIVIVIMGLLISLVAPTLFSKV
                     SSSQRKTTMAQMQMISTALDTYRLDVGSYPDTLDELRRSDRPGWDGPYMPRDIPPDPW
                     GNPYVYSIENQGDAPYSLSSYGAGGLPGGEGDEADIVFQ"
     gene            complement(4019..>4803)
                     /locus_tag="VIW27_02035"
     CDS             complement(4019..>4803)
                     /locus_tag="VIW27_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY5738470.1"
                     /translation="IIAATTIFAIGSIAYALLAKEIYRAEVLLAPAAEQAAPMIGGEL
                     GGLAALAGVSVGEANDVVPLAVLQSREFAREFIEHFDLLPVFFAKEWDAKENRWREDD
                     PAEAPDVRDGVKFFHEKVFEVSEDRTTGLVTLAVEWTDPDVAAEWASILAVRLNDRLR
                     EVALQEAQTNVNYLQSEMAKATLVTMQESIGRLLEAELPKLMLAKGKEEFAFKIVDPA
                     VSPKERVRPRRTLTVIIATILGGLLGVFMVLVGHASRAGSDS"
BASE COUNT          932 a         1590 c         1424 g          857 t
ORIGIN      
        1 gcaccgcatg gaagcgatac tcgacgccga acatggtgcc tgcgccaacg cgctcggctg
       61 ccgggttggc acgcaggtct tcgcggatcg tcggcgtgat ccagggcagt ggcgccagca
      121 gctcggccgt tcgcgcggct cgctcactgg catgcagcgc cgttcgcagc gagagcgtcg
      181 ccgtcgcgag cacggtaaac atgatcacgg ccgcgaccag cacttcgaca agagtgaaac
      241 cccgcaccct gcgcaacttc attcgcggac ctccggcagg atgctcagac tgcgccgccg
      301 gtcgcgctgc aacacctcta tcgccagccg atcggcgatc ccgttcgggt tgatcgtgat
      361 ccgttgttcc ggcgagaaga aaagttgctc gaactcgata acgcccggct gcccgccgcg
      421 ggattcccag gccagtcgtt tgccggcagc ataaacagta acgaagtcac tgcgcaggaa
      481 ggcgtccagc gacaggcgat cgatctccct gtcgagcgtc agccattcgg attgcgcgcg
      541 cgccttctcc acctgctcga tcccgagggg cgccaccagc gtcacgagta tcgaggccag
      601 cagcactacc aggagtattt cgatcagggt aaagccgcgt gccttgcgca acatcagaat
      661 gccgctcccg tgatagatgt gatgctgagc atgatcacca cgaccacggc gccgaccacg
      721 acacccataa aaagaatcag cgccggttcc agcaggctgg tgaaccgctg cgtccactcg
      781 gtgaacgcct ggcggctcct gcctgcgatt tcagcgaata ccggggcaag cgcgccgctc
      841 tcctcgccga cttccagcag ggaggcatag aaatccggaa acagccgcgt ctgtctcaac
      901 accatcgaaa taccctcgcc ctggcgcaat ttgcccagcg cgacgagcag ctcgcggcgg
      961 atcggcgcgc tcttgaccga actgctggca agctgcaggg cccggtccac cggtactccc
     1021 gcctccagca tcatcgccat gccgccggtg aggcgaatcc gctcgaccat gctgatgccg
     1081 tcgcgaagtc ccggcgcatt cgccgcgaat tcgtgccagg ccatttgcat tgcgggccac
     1141 cgcggcaggt aaaagaagat cactgccacg accccgatta tgccgccgag gagcaactgg
     1201 tagcgctgca tgaaatcgct gacgcccatc agcgcctgcg tgtaccaggg cagctccgga
     1261 tagtccgcga acaggctggc caggttcggc acgacgaaat tgaaaatgaa caacagcgaa
     1321 atgacgcata ccgtgaggat gaccagtggg taggtcacgg cggtaacgac cttgcgtcgc
     1381 agatcgcgcc tgaaaccgag gtcatcggcc aggcgcctga aaacggcggc gagacgcccg
     1441 ctggcttcac cgagtgacag caggttgacg tagagcgggt cgaagacctg cggccactcg
     1501 gcaaccgccg ccgagagttg atttccctgc ttcagggatt gcagcaagcc atcgagcagg
     1561 cgatgcgtag ccggattgct ggagccgcgc cgcaggattt cgagcccgcg gtcgatgcgc
     1621 acaccgtttt cgaggagcag cgcaagttcg gcagtgatta tctcgagttg cgcgagtccc
     1681 acccgggact cgccgagccc gagtgagtcg agccagtctt gcgtctctgc cgcgggacgg
     1741 atttcactga ccaggatgtc acgttcgcgc agttccgccc gcgcctgcgg caggctcgcc
     1801 gcttccacgg tgccgttgac gctgctgccc gcggcgtcaa aacccgagta atgaaatcgc
     1861 tgctgcactc aacccgccac ccgaaacact tcctcgaccg tcgtgtctcc gcagagcgcc
     1921 ttcaggagtc cgtcctctac cagggatcgt cctcccagtg acctcatgtg ctcacgagct
     1981 tcggcaatga atcgctcgcc cttgttcatt gccgcgatcg cgtcatcgca gcgcagatac
     2041 tcgacgattg cgagacgtcc gcgatagccg gtgtgaccgc agtcatcaca acccacgctt
     2101 tgccgcagat cgggctccgc caggccggaa cgctcgacga gctctgccag ctggtaacgt
     2161 gccatcagct gcgcggcgtc cggatgcgct gtggagcatt tgcaaagtcg ccgcacaagg
     2221 cgttgcgcga cgatcgaacg cagcgccgcg ttcagcagaa actcctccac ccccagatcg
     2281 agcaagcggg taaaagcgct gggcgcgtca ctggtgtgca ccgtggaaaa aacgaggtgg
     2341 ccggtcagtg cggactgcat tgccacggac gccgtctcgc gatcgcgaat ttcaccaacc
     2401 atgatgacgt cgggatcctg gcgcacgatg ctgcgcaatc cgcgcgcaaa gtcgtaaccg
     2461 atttccggct gcacctggac ctggttgata ccgtcgagct ggtactcgac gggatcttcc
     2521 agcgtgatga tcttcacctc gggactgttg atgcggctca tgaaggtgta caggctcgtg
     2581 gtcttgccgg agcctgtggg cccggtcaaa aggatgacgc ccgaggtcac ctcgaggtct
     2641 tcgaggatgc gtgcttcgat gtcgggcgag agcccgagca ggctcatgtc ataaacaagg
     2701 gtcgccttgc gcaggaatcg cagcactgcg ctctcgccac cttcgtgcag cggcaaagcc
     2761 gaaacgcgga tatcgacttc ctcgccgccg attctcagct cgatcttgcc atcctggggc
     2821 cgcctgcgct cggcgatatc catcgcggca agaatcttga gtcgcgtgat gagcggcagc
     2881 atcatcgccg tgggaatgct ctcgatctcg tgcagcacgc cgtcgacgcg gtaacgcacg
     2941 cgggcccggc ccgcgtgcgg ctcgacgtgc atgtccgatg cgccgcggcg cagggccttg
     3001 gcgatcaagg agttaagcag gttgaccgtc ggcgcctccg aggccatctc gcgcaggcgg
     3061 tcttcctcga ccagcgacaa gccatccagc tgcgaatcgc tcttgccgat gccgagacgt
     3121 gaccgcagcg tctcgaagtc atccgctgaa agcagccgca acgcgggtcg cagagcatgc
     3181 tcggcaaacc aggcgttgac ttcgaggtcg aacggcgagc agctggcaaa cacccagctg
     3241 tctgcgtcgc gttcccatgg caaccagccg cgttccagca ggaattccag cggcagcgcg
     3301 tcgctgcctt cgggtaactg ccagtcacgc cagtctgcct cggacagtac cggggcgccg
     3361 aaccacttgg aatagatggc cggcaggtcc tcggccggca ggctgcccat gttcaccagt
     3421 atctgctcca gccgcccgcc gtgcttggcc tggtaagagc gcgccttttc gagatccgcc
     3481 gccgggaccc cgtgctctga aaaaacgtct aacacggcgc tcactggaac acgatgtccg
     3541 cttcgtcacc ctcgccgccg ggaagcccgc ctgccccgta agaagacagg ctgtagggcg
     3601 catcgccttg gttctctatc gaatagacgt agggattgcc ccacggatcg ggcggaatgt
     3661 ctcgcggcat atacggccca tcccagcccg gacgatcgct gcgccggagt tcgtccaggg
     3721 tgtccggata cgaaccgacg tccagccgat aggtatcgag cgcggtcgaa atcatctgca
     3781 tctgtgccat cgtggtcttg cgctgcgagg acgacacctt cgaaaacagg gtcggggcca
     3841 cgagtgagat cagcagcccc atgatcacga tgacaatcaa tatttcaatc agcgtaaaac
     3901 cgccgctctg tcgccgctcg atgccctgga tagcctttga tttcattgcc gaatccgctg
     3961 caagaacaag atcgttcagc ataccctatc cgccgaggag tgtggtgggc cggggctctc
     4021 aggaatcgct gccggctcga cttgcatggc cgaccagcac catgaaaacg cccagcaaac
     4081 caccgagtat ggtggcgatg atcacggtca aggtgcgcct tggacggacc cgctccttgg
     4141 gcgagacggc cgggtccacg atcttgaaag cgaattcctc tttgcctttt gccagcatca
     4201 gcttcggcag ctcggcctcg agcaggcgcc cgatagactc ctgcatggtg accagcgtgg
     4261 ccttcgccat ctccgactgc aggtaattca cgttggtctg cgcctcctgc aacgccacct
     4321 cgcgcagtcg atcattgaga cgcaccgcga gtatgctcgc ccattcagct gcaacatcag
     4381 gatcggtcca ctcgacagcg agcgttacca gtccggtcgt ccggtcctcg ctgacctcga
     4441 aaactttttc gtggaaaaac ttcacgccat cccgcacgtc cggcgcctct gccgggtcgt
     4501 cttcccgcca gcgattttct tttgcatccc attcttttgc aaaaaagacc ggcagcagat
     4561 cgaaatgctc gataaattcc cgggcgaatt cccgcgactg cagcaccgcc agcggcacga
     4621 catcgttcgc ctcgcccacg ctcacgcctg ccagtgccgc gagaccgccc agctcgccgc
     4681 cgatcatcgg cgctgcctgt tcggccgcag gcgcgagcag cacctcggcg cggtagatct
     4741 ctttcgccag caacgcgtag gcaatcgagc caatggcaaa gatcgtcgtc gccgcgatta
     4801 tcg
//
LOCUS       DATCZV010000089         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_430053, whole genome shotgun sequence.
ACCESSION   DATCZV010000089 DATCZV010000000
VERSION     DATCZV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430053"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VIW27_02040"
     CDS             complement(<1..500)
                     /locus_tag="VIW27_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5738471.1"
                     /translation="MKATARSLFLAALVAICVPGLALCDQTDSRLDALFETLQSSQDE
                     VLLLETESEIWDIWYDSGEQQVDDKMEQAAEQLRNGNLAAAGRLYSEVIEMLPEFSEG
                     WNRRATVRFYQRDYAGSLADIEETLRLEPRHFGAIWGLGMILGSQRDYERAILAFERL
                     LEIKPNA"
     gene            674..2119
                     /locus_tag="VIW27_02045"
     CDS             674..2119
                     /locus_tag="VIW27_02045"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY5738472.1"
                     /translation="MFDLLKQACEAARRAGCEFADARYLDIRRQRITSRDHALSSCSD
                     SDDRGFGVRVLYRGAWGFASSPGGDAAEITRIVELAIRIARVSALAARPGGVRWAAEN
                     PLSMEFHSPCELDPFAVGLEDKAGLLLSANEVMLRNDSIKRALGFLHFKRVRRWYVNT
                     DGSELHSDVTTSEAVLSAIAVDASDFQQRNWQAPASSRGFESFSTAEFERQAERIADE
                     AEQKLTAKLAPEGEFDIVTDGENLSLTIHESVGHATELDRVLGYEVSMAGSSFVSRDK
                     LGEFRYASDAVTLVADNTLDGGLATQGFDDDGVPGQCWDIVTDGIFRGYSTGREFAHF
                     VGEERSRGCCRADHWSSIPIVRIPNLYLQPGKRPLDPDELLADIRHGLYFEGMDSFSI
                     DQWRLNFQFGGNAVWEIRNGKKAGMVKAALYQSQTPVFWNACDAVCDQHHWSPHGVTN
                     CGKGDPMQITQMTHGAAPARFRGIRICRGRD"
     gene            2119..3408
                     /locus_tag="VIW27_02050"
     CDS             2119..3408
                     /locus_tag="VIW27_02050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5738473.1"
                     /translation="MERQQAEDLIAGILEMPHPGELQVAIHGVNRLGTRFNDCAISQN
                     ALRDQVTLTLSGRLEQKKTSLTTNNLGDRDAIRAAIEQLFATCEHLPDDEESMPAPGE
                     RPDCPEHAFSETGEALEIETVGAWVSKACAAGKRAGIDLAGLLSVGQMYFAYGDSNGG
                     FAFERSHRCDFHVTASGARGSGWAEHQGVTIDRPQIVAATERAIDKCLRAQNPVVFEP
                     RPTTVVLEPQAVGDLLSSAFWYGFDQRAADEGRSAFANFDRRLGHLSLYSDPAEPLAP
                     ANSITADGQALGRCLWLDRGELQQLRTSRFWAERKRLSPKHTPNNLILEGRGVPLEEL
                     IAGTEDGVLVTRFWYIRSTDASTLGFTGMTRDGTYRIENGQLTAPLVDMRWNESALRV
                     LENVVDSGRPVATGSFPAMAMPALKVEDFHFSSLSGR"
     gene            3510..>3648
                     /locus_tag="VIW27_02055"
     CDS             3510..>3648
                     /locus_tag="VIW27_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY5738474.1"
                     /translation="MLPLNLSANEIRVIGCLMEKSVTTPEQYPLTLNALTNACNQKSS
                     RN"
BASE COUNT          686 a         1190 c         1125 g          647 t
ORIGIN      
        1 gcatttggct tgatttcgag caaccgctca aaggcgagaa tcgcgcgctc gtagtcgcgc
       61 tgtgaaccga gaatcatacc gagcccccag atggcaccga aatggcgcgg ctcgagccgc
      121 agcgtttcct cgatatcggc cagcgagccg gcgtaatcgc gctggtagaa gcgcaccgtg
      181 gcgcgccggt tccagccctc ggaaaactcc ggcagcatct cgatgacctc ggagtagagc
      241 cgcccggcgg ccgccagatt gccattgcgc agctgttcgg cggcctgctc cattttgtcg
      301 tcgacttgct gttcgccgct gtcgtaccag atatcccaga tttcgctctc ggtctccagc
      361 agtagcacct cgtcctggct gctttgcagg gtctcgaaca gggcgtcgag acgcgaatcg
      421 gtctggtcgc agagcgccaa acccggcaca cagattgcca ccagggcggc caaaaacaat
      481 gaccgggctg tcgctttcat gccgttcctc ccgcgcgtga catcggtgta atgctaacag
      541 tgtttgcgac cgggttctaa ttgaacggct gtcggcgagc ggcgcggcgc gctctgctgc
      601 cggcaaatat catcgccttg aaataccggc ctgcccggca accaaacagc gacggttatg
      661 ctagagtaat tcgatgttcg atttgctcaa gcaggcctgc gaagccgccc gccgcgccgg
      721 ctgcgagttc gccgatgcgc gctatctcga tattcgtcgc cagcgaatca cctcgcgaga
      781 ccatgcgctg tcgtcatgca gcgacagcga cgatcgcggc ttcggtgtgc gcgtgctgta
      841 tcgcggtgcc tggggattcg cgtcgtcgcc gggcggcgat gccgcggaaa tcacgcgcat
      901 cgtcgagctg gcgatacgca tcgcccgggt atcggcgctg gccgcgcgtc ccggcggcgt
      961 gcgctgggct gctgagaatc cgctgtcgat ggaattccac tcgccctgcg agctcgaccc
     1021 gttcgcggtc ggcctcgagg acaaggccgg gctgctactg tccgccaacg aggtcatgtt
     1081 gcgcaacgat tcgatcaagc gcgcgctggg attcctgcac ttcaagcgcg tgcggcgctg
     1141 gtacgtcaac accgatggca gcgagctgca cagcgacgtg accacctccg aggcggtgct
     1201 ttcggcgatt gccgtcgatg ccagtgattt ccagcaacgc aactggcagg cacccgccag
     1261 cagccgcggt ttcgaaagtt tctccaccgc cgagttcgag cgccaggccg agcgcatcgc
     1321 cgacgaggcc gagcaaaagc tgaccgcgaa actggcaccc gagggcgaat tcgatatcgt
     1381 caccgacggc gaaaacctgt cactgacgat tcatgaatcc gtcggccacg caaccgagct
     1441 cgaccgcgtg ctgggctacg aggtatcgat ggccggctcg tcgttcgtca gccgcgacaa
     1501 gctcggggaa tttcgctacg ccagcgacgc ggttacgctg gtagccgaca atactctcga
     1561 cggcgggctc gcgacccagg gtttcgacga cgacggtgta cccgggcagt gctgggatat
     1621 cgtcaccgac ggcatctttc gtggctacag caccggccgc gaattcgcgc atttcgtcgg
     1681 tgaggaacgc agccgcggct gttgccgcgc cgatcactgg tcgagtattc cgatcgtacg
     1741 catccctaat ctctacctgc agcccggcaa gcgcccactc gatcctgacg agttactggc
     1801 cgacattcgc catggcctgt atttcgaggg catggacagt ttttccatcg accagtggcg
     1861 gctgaatttc cagttcggcg gcaacgcggt gtgggaaatc aggaacggca aaaaggccgg
     1921 catggtaaaa gccgcgctct accaaagcca gacgccggta ttctggaacg cctgtgacgc
     1981 ggtctgcgat cagcaccact ggagcccgca cggcgtcacc aactgcggca agggtgatcc
     2041 gatgcagatc acgcaaatga cccacggcgc ggcgccggca cgcttccgtg gcatccgtat
     2101 ctgccgggga cgcgactgat ggagcgccaa caggcagagg acctgattgc cggcattctc
     2161 gagatgccgc acccgggtga attgcaggtc gcgatccacg gggtaaatcg tctcggtacc
     2221 cgcttcaacg attgcgcgat ttcgcaaaac gcgctgcgcg accaggtgac cctgaccctg
     2281 agcgggcgac tggagcagaa gaagacctcg ctgacgacca acaatctcgg cgaccgcgac
     2341 gcgatccgtg cggcgatcga gcaattattc gcgacctgcg aacacctgcc cgacgacgag
     2401 gaaagcatgc ccgcgcccgg cgagcgcccg gactgccccg agcacgcctt cagcgaaacc
     2461 ggcgaggctc tcgaaatcga aaccgtcggc gcctgggtgt ccaaggcctg cgcagcgggc
     2521 aagcgggccg gaatcgacct cgccggcctg ctgtcggtcg gccaaatgta tttcgcttac
     2581 ggcgacagca acggcggttt cgcctttgag cgcagccacc gctgcgactt tcacgtcacc
     2641 gccagcggtg cccgcggcag cggctgggca gagcaccagg gagtgactat cgatcggccg
     2701 caaatcgtcg ccgctaccga gcgcgcgatc gacaagtgcc tgcgtgcgca aaacccggtc
     2761 gtcttcgagc cccggccgac taccgtggta ctcgaaccgc aggcagtcgg cgacctgctg
     2821 tcgtcggcct tctggtatgg cttcgaccag cgcgccgccg acgagggccg ctcggcgttt
     2881 gccaacttcg accggcgtct cggccatctg tcgctgtatt ccgatcccgc cgagccgctg
     2941 gcgccggcga attcaatcac cgccgacggc caggcgctcg gccgctgcct gtggctggac
     3001 aggggcgagt tacagcaatt gcgcaccagc cgcttctggg ccgaacgcaa gcggctgtca
     3061 ccaaagcaca cgcccaacaa cctgattctc gagggccgcg gcgtaccgct cgaagagctg
     3121 atcgccggca ccgaggacgg tgtgctggta acccgtttct ggtatatccg ctctaccgat
     3181 gccagcactc tcggttttac cggtatgacg cgcgacggta cctatcgcat cgaaaacggc
     3241 caactgacgg cgccactggt tgatatgcgc tggaacgaat cggcgttgcg agtgctcgaa
     3301 aacgtcgtcg acagcgggcg cccggtggcc accggcagtt ttccggcaat ggcaatgccg
     3361 gcgctgaagg tcgaggattt tcatttctcg tcgctgtcgg gtcgctgatc cgtggcccgt
     3421 gttcggaatg attccgggcc ggggctgctt tgggctaaaa tcatcggcga aaccggtcac
     3481 cgccattacc acctggagaa aacaacacga tgctgccgct aaacctgtcc gccaacgaga
     3541 tccgcgtcat cggctgcctg atggaaaaat ccgtcaccac gccggagcag tatccgctga
     3601 ccctgaacgc gctgaccaac gcctgcaacc agaaatcctc gcgtaacc
//
LOCUS       DATCZV010000090         2981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_270903, whole genome shotgun sequence.
ACCESSION   DATCZV010000090 DATCZV010000000
VERSION     DATCZV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270903"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="eutB"
                     /locus_tag="VIW27_02060"
     CDS             <1..957
                     /gene="eutB"
                     /locus_tag="VIW27_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyectoine utilization dehydratase EutB"
                     /protein_id="HEY5738475.1"
                     /translation="LGDIEAARERIAPRIVPLHIAPSASLSRRLERDILLAHEYLQTT
                     GAFKLRGAMNAVLQLPSATSGVTAVSTGNHGRALAYAARQAGVPCVICMSKLVPGNKL
                     EAIRALGAETRIVGKSQDEAEVEARRLVEEEGYTMLPPFEHPHIIAGQGTLGLEILEQ
                     VPDVDTVLVQLSGGGLISGIALAIKLQRPGVRMVGISMQRGAAMYASQQAGEPIEVEE
                     LPTLADSLGGGIGLDNRLTFGIVRDYVDELVLLSEAEIADGIRHAYFEEQTVLEGGGA
                     VGIAALLAGKVEPGDKTVVLLSGRNIDMELHRKIISHEDYEL"
     gene            960..1952
                     /locus_tag="VIW27_02065"
     CDS             960..1952
                     /locus_tag="VIW27_02065"
                     /inference="COORDINATES: protein motif:HMM:NF006141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase"
                     /protein_id="HEY5738476.1"
                     /translation="MADIRILTEAELRGQIQLDLAVVDCVENAFHALATKSVVMPPIM
                     RLDILENNGEIDVKTAYIPGVDSLAIKISPGFFNNPSLGLPSVNGLMVLFSTRTGMVE
                     ALLLDNGYLTDLRTAAAGAVAAKYLSRADSRRAAILGAGVQAGLQLEALTLVRDIESA
                     SIWARDPGRAAAAAEKLTRRLGIPVSACAEVAAATAGADIVVTTTPANSPLLEPQHLE
                     AGQHVTAMGSDAEHKNEIDPRIIAGETRYFCDRLSQVRALGELHHAIDQGLVAADTEF
                     TELGQVIAHQRPGRDTEDEITLCDLTGTGIQDTAIATLAFSLCQQQQAGTQFTT"
     gene            1964..>2981
                     /locus_tag="VIW27_02070"
     CDS             1964..>2981
                     /locus_tag="VIW27_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY5738477.1"
                     /translation="MPKDTKINFTVDEYAARLAGTRQAMARQDIDCLVVVDPSNMAWL
                     TGYDGWSFYTHQCVIVQHDAEPIWWGRGIDAAGALRTVYLAEDNIIGYPDHYVQSTEY
                     HPMQHLAELLARLGHGKAAIGVELENYYYSAAAHQALQADLPGARLVDATALVNWQRL
                     VKSPQELEYMRAAARIVESMHAAIYEMIEPGLKKNLLAAEIFRSGVAGTDDYWGDYPA
                     ILPMLPTGMDATAPHLTWDDSVLAAGDITFFEIAGCYRRYHCPLSRTIALGPPPQKYL
                     DAEKAVLEAMHAGLDNAKPGKLCEDVAIAFFDTLEKHGFKKDNRTGYSIGLSYPPDWG
                     ERTIS"
BASE COUNT          580 a         1001 c          916 g          484 t
ORIGIN      
        1 ctcggcgata tcgaggcggc gcgcgagcgc atcgcgccga ggatagtgcc actgcacatt
       61 gcgccgtcgg cgtcattgtc gcgacgcctc gagcgcgaca tcctgctggc gcatgaatac
      121 ctgcagacca ccggcgcctt caagctgcgc ggtgcgatga acgcggtgct gcaactaccg
      181 tcggcgacca gcggtgtcac cgccgtatct accggcaacc acggccgcgc gctcgcctat
      241 gcggcacgcc aggcgggggt cccgtgcgtg atttgcatgt ccaaactggt acccggcaac
      301 aagctcgagg cgattcgagc gctcggtgcc gagacccgaa tcgtcggcaa gtcgcaggac
      361 gaggccgagg tggaggccag acgcctggtg gaagaagaag gctacaccat gctgccgccg
      421 tttgaacatc cacacatcat tgccggccag ggaaccctgg gcctggaaat tctcgaacag
      481 gtacccgacg tcgatacggt gctggtgcaa ctttcgggtg gcggcctgat ctccggcatc
      541 gcactcgcca tcaagctgca gcgcccgggg gtgcgcatgg tcggcatttc gatgcagcgc
      601 ggcgcggcca tgtacgcgtc gcagcaggcc ggcgaaccga tcgaggtcga ggaattgcca
      661 actctcgccg attcgctcgg cggcgggatc gggctcgaca accggctcac cttcggtatc
      721 gtacgcgatt acgtcgacga gctggtgctg ttgagcgaag cggagatcgc cgacggcatt
      781 cgccacgcct acttcgagga acagaccgta ctcgagggcg gcggcgcggt cggcatcgcg
      841 gccctgctgg ccggcaaggt cgaaccgggg gacaagaccg tcgtgttgct gagcgggcgc
      901 aacatcgaca tggaattaca ccgcaaaata atcagtcacg aggattacga actctagaca
      961 tggccgacat cagaatattg accgaggccg aattacgcgg ccagattcag ctcgacctgg
     1021 cggtcgtcga ctgcgtcgag aacgcctttc atgcgctggc caccaagtcg gtggtgatgc
     1081 cgccgatcat gcgcctcgac atcctcgaaa acaacggcga aatcgatgtc aagaccgcct
     1141 acattcccgg tgtcgacagt ctcgcgatca agatcagtcc cgggtttttc aataacccga
     1201 gtctcggatt gccgagcgtc aacggcctga tggtgctgtt cagcacgcgc accggcatgg
     1261 tcgaggcgct gttgctcgac aacggctacc tgaccgacct gcgcaccgcc gccgccggcg
     1321 cggtcgccgc gaaatacctg tcgcgggccg acagccggcg agcggcgata ctcggcgccg
     1381 gcgtgcaggc gggtctgcaa ctcgaggcac tgaccctggt acgcgacatc gagtccgcga
     1441 gcatctgggc gcgcgacccc ggccgtgccg cggccgccgc cgagaagctc acccggcggc
     1501 tcggcattcc ggtcagcgcc tgcgccgagg tcgcggcggc gaccgcaggt gccgacatcg
     1561 tcgtcaccac gaccccggcg aacagcccgc tgctcgaacc gcaacacctc gaggccggcc
     1621 agcacgtcac cgcgatgggt tccgatgccg agcacaagaa cgaaatcgat ccgcgaatca
     1681 tcgccggcga gacgcgttac ttctgcgacc gtctgtcaca ggtccgcgcg ctcggtgaat
     1741 tgcaccacgc gatcgaccaa ggcctggtgg ccgcggatac cgagttcacc gaactcggcc
     1801 aggtcatcgc ccatcagcga cccgggcgcg ataccgagga tgaaatcact ctctgcgacc
     1861 tgaccggtac cggtatccag gacaccgcga tcgcgaccct ggctttcagt ctgtgccagc
     1921 aacagcaggc cggcacccag ttcaccacct gagtttcaag cccatgccga aggacacaaa
     1981 aatcaatttc accgtcgacg aatacgccgc gcgactcgcc ggcacccgcc aggcgatggc
     2041 caggcaggat atcgattgcc tggtggtcgt cgacccgtcc aatatggcct ggctgaccgg
     2101 ctacgacggc tggtccttct atacccatca atgcgtgatc gtgcagcacg acgcggaacc
     2161 gatctggtgg ggtcgcggaa tcgatgccgc cggtgcgctg cgcacggtct atctcgccga
     2221 ggacaacatc atcggttacc cggatcatta cgtgcagtcc accgagtacc accccatgca
     2281 gcacctcgcc gaattactgg cgcgcctcgg tcacggcaag gcggcgatcg gcgtcgaact
     2341 ggaaaattac tattattccg ccgccgcgca ccaggcgctg caggccgacc tgccgggcgc
     2401 caggctggtc gacgcgaccg cgctggtcaa ctggcagcgc ctggtcaaat caccgcagga
     2461 gctcgaatac atgcgcgccg cggcgcgcat cgtcgaatcc atgcatgcgg cgatttacga
     2521 gatgatcgaa ccgggactga agaagaacct gctggccgcg gaaatctttc gcagcggtgt
     2581 cgccggtacc gacgactact ggggcgacta cccggcgatc ctgccgatgc tgcccaccgg
     2641 catggatgcc accgcgccgc acctgacctg ggacgattcc gtgctcgccg ccggtgatat
     2701 cacctttttc gagatcgccg gctgttatcg gcgctatcac tgcccgttgt cgcgcaccat
     2761 tgcgctcgga cctccgccgc aaaagtacct cgatgccgaa aaggcggtgc tcgaggcgat
     2821 gcacgccggc ctcgacaacg ccaaacccgg caagctctgc gaagacgtcg ccatcgcgtt
     2881 tttcgacacc ctcgaaaaac acggcttcaa gaaggataac cgcaccggtt actcgatcgg
     2941 gctatcctac ccgcccgact ggggcgagcg caccatcagc c
//
LOCUS       DATCZV010000091         4972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_641214, whole genome shotgun sequence.
ACCESSION   DATCZV010000091 DATCZV010000000
VERSION     DATCZV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641214"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VIW27_02075"
     CDS             <1..624
                     /locus_tag="VIW27_02075"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809854.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY5738478.1"
                     /translation="LYQDARKRYQEQGDVEGLSIGRALLESQQNISLSDRERLLGYLD
                     GEGRIILPEPQSLLTVSSKMPGIDGQKMSKTYNNTIALREDPDEVERKIRTMPTDPAR
                     VRRTDPGDPEKCPVWEFHQVYTDDETCKTLDEGCRTAAIGCIDCKQYIIDAIQAELKP
                     IQQRVREYLGDLGAVDSIITEGCEAARDVAQDTIEEVRKVMGLTGRK"
     gene            624..1430
                     /locus_tag="VIW27_02080"
     CDS             624..1430
                     /locus_tag="VIW27_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY5738479.1"
                     /translation="MRRQPSETSSHQSEMPFALVQGEPLTVIPQDLYIPPDALLVFLE
                     AFEGPLDLLLYLIKRQNIDILNIPIAAITRQYMEYIALMETLELELAAEYLLMASMLA
                     EIKSRMLLPRPAQSEDEDDPRAELVRRLQEYEQFKQAAEDLDALDRLERDIHLTSIDI
                     PKFDIDIPLPEVSLGDLMSAFRDVVNRAEQFASHHVEREPLSVRERMVIVLDRINAEK
                     FTSFNDFFDPAEGRMGVVVSFIAILELLKESLIELIQNEPFAPIYVKARS"
     gene            1435..2181
                     /gene="scpB"
                     /locus_tag="VIW27_02085"
     CDS             1435..2181
                     /gene="scpB"
                     /locus_tag="VIW27_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY5738480.1"
                     /translation="MQVERLKPILEALFAASDRPLSVNQIFELFVGDVEQPGKDEIRQ
                     AIQELTQKYQDSGFELKQVASGFRFQVKTDFETWVARLWEQKPPRYSRALMETLALIA
                     YRQPITRGEIEDIRGVSVSSNIIKTLQEREWVKPLGHKEVPGRPTLYGTTREFLDYFN
                     LKSLNEMPTLSEIRDLDRFNPEFAFDEAENAQSAQPGDVEGAETMQPGDIDDVETARP
                     GEAEAAEIASSDEAISSAAEGDEEVEAAVN"
     gene            2491..3270
                     /locus_tag="VIW27_02090"
     CDS             2491..3270
                     /locus_tag="VIW27_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5738481.1"
                     /translation="MERIQKFLANQGAGSRRQIDSLLQEGRISVNGKPAKPGDQVAGG
                     EKIAIDGKLLRLQRHVARPRLLMYHKPIGQVCSRADPQGRDDVYQHLPGLQHGRWVGI
                     GRLDINTSGLLLFTNDGELANRLMHPSYEVEREYAVRVHGEVSAEMLQRLRDGVELDD
                     GPAHFDDILDSGGSGRNHWYHVVLKEGRNREVRRLWEAVGVEVSRLVRVRYEEFTLPK
                     WLKPGKSRVLDEAVVERVYKRLGLKPARPAPRKAARRRRRA"
     gene            3270..3710
                     /locus_tag="VIW27_02095"
     CDS             3270..3710
                     /locus_tag="VIW27_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738482.1"
                     /translation="MTGRKISRLCQSVLPPGYERVERLAPALQRFLEENLPAPINRAV
                     SLLTVVDDEIVIAAYNPVVANYLRLHGKEIAQQIRETLGFDARLRFRTLPESMLRAGP
                     HVRERKPRTVSAASIDAVRRNAEWIEDEELRAALLSLAQSMQSD"
     gene            4305..4787
                     /locus_tag="VIW27_02100"
     CDS             4305..4787
                     /locus_tag="VIW27_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5738483.1"
                     /translation="MTIHSSLAILLLLPAAMALEVQAQTVNKWVDDEGVTHFSDQKPA
                     GSDADVREIEIPKGSVSQFDAREVNERVNSVLQQLEQDRKAREAEAAARIRVREEEQA
                     LELEAVVEENKQKKKKKRNRPYYGPYPKPLPGPFPEQYPRQFGPAVPSNPIAPDTGNS
                     "
BASE COUNT         1009 a         1594 c         1570 g          799 t
ORIGIN      
        1 ctctaccagg atgcgcgcaa gcgctaccag gagcagggcg atgtcgaggg gctgtcgatc
       61 ggccgcgcgt tgctggaatc gcagcagaat atcagcctca gcgatcgtga gcggttgctc
      121 ggttacctcg acggcgaggg ccgcatcatc ctgcccgagc cgcagtcgct gctgacggtg
      181 tcgtcgaaaa tgcccggcat cgacgggcag aagatgtcca agacctataa caacaccatc
      241 gcgctgcgcg aagaccccga cgaggtcgaa cgcaagatca ggaccatgcc gaccgatccg
      301 gcgcgcgtgc ggcgcaccga ccccggcgac ccggagaagt gcccggtgtg ggaattccac
      361 caggtttaca ccgacgacga gacctgcaag acgctcgacg agggctgtcg caccgcggcc
      421 atcggctgca tcgactgcaa gcagtacatc atcgacgcga tccaggccga gttgaagccg
      481 atccagcagc gggtgcgcga atacctgggt gacctcggcg ccgtcgacag catcatcacc
      541 gagggttgcg aggcggcccg cgacgtcgcc caggacacca tcgaggaagt gcgcaaggtc
      601 atgggcctga cggggcgcaa gtgatgcggc ggcagccctc cgaaacgtct tcgcaccagt
      661 ccgagatgcc gttcgcgctg gtccagggcg agccgctgac ggtgattccg caggacctgt
      721 atattcctcc ggatgcgttg ctggtattcc tcgaggcctt cgagggtccg ctcgacctgc
      781 tgctctacct gatcaagcgg cagaatatcg atattctcaa tattccgatt gccgcgatca
      841 ctcgccagta catggaatat atcgcgctga tggaaaccct cgagctggag ctcgccgccg
      901 agtacctgct gatggcgtcg atgctggccg aaatcaagtc acgcatgctg ttgccgcggc
      961 ccgcccagtc cgaggacgag gacgatccgc gcgccgagct ggtacgccga ctgcaggaat
     1021 acgagcagtt caagcaggcc gccgaggatc tcgacgcgct cgaccgcctg gagcgcgata
     1081 ttcatctcac cagtatcgat attccgaaat tcgatatcga cataccgctg cccgaggtga
     1141 gcctcggcga cctgatgagc gcgtttcgcg acgtggtcaa tcgcgccgag cagttcgcca
     1201 gccaccacgt cgagcgcgaa ccgctgtcgg tgcgcgagcg catggtcatc gtgctggatc
     1261 gaatcaatgc cgagaaattt accagcttca acgatttctt cgaccccgcc gagggccgca
     1321 tgggcgtcgt ggtcagcttt atcgcgattc tcgagctgct caaggagtcg ctgatcgaac
     1381 tgattcaaaa cgaacccttc gccccgatat acgtcaaagc caggtcctga tccgatgcaa
     1441 gtcgaacgcc tcaagccgat actcgaagcc ctgttcgcgg ccagcgacag gccgctgtcg
     1501 gtgaaccaga tcttcgagct gttcgtcggc gacgtcgagc agccgggcaa ggacgagatt
     1561 cgccaggcga tccaggaact gacgcaaaag taccaggaca gcggcttcga gctgaagcag
     1621 gtggcatccg ggtttcgctt ccaggtcaaa accgacttcg aaacctgggt cgcgcgcctg
     1681 tgggagcaaa agccgccgcg ttactcgcgc gcgctgatgg aaacgctggc gctgatcgct
     1741 taccgccagc cgatcacgcg tggcgagatc gaggacattc gcggcgtcag cgtcagctcc
     1801 aacatcatca agacgctgca ggagcgcgaa tgggtcaaac cgctgggcca caaggaggtg
     1861 ccgggccgac cgacgctgta cggcaccacg cgcgagtttc tcgattattt caatctgaaa
     1921 tcgctcaacg aaatgccgac cctgtccgag attcgtgatc tcgaccgctt taaccccgag
     1981 tttgctttcg acgaggccga gaacgcccaa tccgcgcagc ccggtgacgt cgagggcgcc
     2041 gaaaccatgc agcccggcga tatcgacgac gtcgaaaccg cgcggcccgg cgaagccgaa
     2101 gccgccgaga ttgcttctag cgacgaggcg atttcctccg cagcagaggg cgacgaagaa
     2161 gtcgaggccg ccgtcaacta gcccgcccgt caccaaagca ccgtcatgcc ggcccccgag
     2221 ccggcatctt tccagccccc tgaggcaggc tcacctggat gcaaagagat tgcggctcga
     2281 ggccgcaatg acgcgactgt cgcgatgagg cgactgcctc ggcgcgaggc cgtaatgacg
     2341 tgacggcgag gtaatccagc atagcgtcat gccggccccc gagccggcat ctttccgacc
     2401 ctgtgctgca cgatcgaaat attattgagg attgcatcga accgcggcat ccagtccact
     2461 gcaggccttc accccgtgta taattcgccg atggaacgca ttcagaaatt cctcgccaac
     2521 cagggggctg gctcccggcg gcagatcgac agcctgctgc aggagggtcg gatcagcgtc
     2581 aatggcaagc cggcaaagcc cggcgaccag gtcgccggcg gcgagaagat cgccatcgat
     2641 ggcaagctgc tgcgcctgca gcggcacgtc gcgcgcccca gactgctgat gtatcacaag
     2701 ccgatcgggc aggtctgctc gcgcgccgat ccgcagggac gcgacgatgt ctaccagcac
     2761 ctgccgggcc tgcagcatgg ccgctgggtc ggcatcgggc gcctcgatat caacaccagc
     2821 ggcctgctgc tgttcaccaa cgacggcgag ctcgccaacc gcctgatgca cccgtcttac
     2881 gaggtcgagc gcgagtacgc ggtgcgcgtg cacggcgagg tcagcgccga aatgctgcag
     2941 cgcctgcgcg acggcgtcga actcgacgac ggtccggcgc acttcgacga tattctcgat
     3001 tccggcggca gcggccgcaa ccactggtac cacgtggtgc tgaaagaggg ccgcaaccgc
     3061 gaggtacgac gcctgtggga agcggtcggc gtcgaggtca gccggctggt gcgcgtgcgc
     3121 tacgaggagt tcacgctgcc gaagtggttg aagcccggca agtcccgcgt gctcgacgag
     3181 gcggtcgtcg agcgggtcta caaacgcctc ggcctgaaac ccgccaggcc ggcaccgcgc
     3241 aaggccgcgc gccgccgacg ccgggcgtga tgaccggccg caagataagc cgtctgtgcc
     3301 agtcggtttt gccgccgggc tacgaacggg tcgagcggct ggcgcccgcg ctgcagcgat
     3361 tcctggagga aaacctgccc gcgccgatca atcgggcggt ctcgttgctg accgtcgtcg
     3421 acgatgaaat cgtgatcgcc gcctacaacc cggtagtggc gaattacctg cgcctgcacg
     3481 gcaaggaaat cgcgcagcag attcgcgaga ccctcggctt cgatgcgcgc ctcaggtttc
     3541 gcacgctgcc ggagtcgatg ctgcgggcgg gaccgcacgt gcgcgagcgc aagccgcgaa
     3601 cggtcagcgc ggcgtcgatc gacgcggtca ggcgcaacgc cgaatggatc gaggacgaag
     3661 agctgcgtgc cgcgttgctg tcgctcgcgc aaagcatgca gtcggattaa ccgcagcccg
     3721 gcaacgtcga tcggcgtgtg ccggcgaatc agttgcaacc gccgcccgcg catgccgccc
     3781 cgacaaccct gccgcaattt gggtcagatc aaaatttcca cggctcgcac ccgatgccgc
     3841 gatcggcggg cagatcgacg aattcccgaa gccaggatca gcactcgata gccataatcg
     3901 ataacgccga aggtcggcgt gccgctcgcg ccagcagtcg cgcgttaaac gctgtttgcc
     3961 cgaggcgtgt cccctgggct cctgtgtctt ccgggctccc caccagggcc gacttgtttt
     4021 cggcagggac gaatatcgtc gcccgctgcg ctatcgccat tgacaacctc gtctcgacgg
     4081 gagcgatcga gcgggcgtgg tcagtatttt cgggagtacg gggtcggctg gcaatcagca
     4141 cgagactcga attgtcgtta agggctgcgg gattcgtgca gccgacccgt tagggacttg
     4201 cgcggaagat ttatcgaaac aggcgcttaa atcgcggatt tatctataat caaaggcgtc
     4261 ggcggcgaca atcgattcgc accctcggtg gtcgagaata caggatgaca atacactcaa
     4321 gtttagcgat cttgctgctg ttaccggcag ccatggcact cgaggtgcag gcacagacgg
     4381 tgaacaagtg ggtcgacgac gagggcgtca cgcatttttc cgatcaaaag cccgccggca
     4441 gcgatgccga tgtcagggaa atcgaaatac cgaagggatc ggtatcgcag ttcgatgcac
     4501 gggaagtgaa cgaacgcgtc aacagcgtgc tgcagcaact cgagcaggac cgcaaggctc
     4561 gcgaagcgga agccgcggcg cgcatcaggg tcagggaaga ggagcaggcg ctcgagctcg
     4621 aagccgtcgt cgaggaaaac aaacagaaga agaaaaagaa acgcaaccgt ccgtactacg
     4681 gaccctaccc gaagccgctg ccgggaccct tcccggaaca gtacccgcgc cagttcggcc
     4741 cggccgtgcc gagcaacccg atcgccccgg ataccggcaa cagttagctg cgccgcctgc
     4801 tgccgctgcc gatcattcag ccgccgcgaa acctgccgcg cgccgtcccc ggttagccag
     4861 gggaatacga gctcatgact ggcgcggcgc ctgcgcgagt cgcgccagcg tatcgctcat
     4921 gccgccgacg ccggcggtcg accagccgcc gtcgaccggc agcacgacgc cg
//
LOCUS       DATCZV010000092         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_6411, whole genome shotgun sequence.
ACCESSION   DATCZV010000092 DATCZV010000000
VERSION     DATCZV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6411"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VIW27_02105"
     CDS             complement(<1..505)
                     /locus_tag="VIW27_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5738484.1"
                     /translation="MLLPIALLLGCEPRQQLVEQHLLEFGTLIQISLVTDDLSRAEGL
                     LNDIEIRLRQQRGQWHAWEDSDLTRFNDALRRSGSAPLPDSLEPLLRLSRGYYDSSGG
                     LFNPALGKLIAAYGFHGAPPDPAAIRALKRDLPGMHDLVIDGGRALSRHPDLQIDLGG
                     IAKGYAVG"
     gene            complement(514..1470)
                     /gene="gshB"
                     /locus_tag="VIW27_02110"
     CDS             complement(514..1470)
                     /gene="gshB"
                     /locus_tag="VIW27_02110"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY5738485.1"
                     /translation="MPSQRKLGIVMDPIEAIKSWKDTTMAMMLEARKRGWQIHYFTLD
                     DVRLDNGIARGRFRIVEAHDDADHWFDYTDDGDCRLDELDAILMRKDPPFDLEYVYCT
                     YILEQAEENGVLVVNRPASLRDCNEKLYTRYFPQCCVETLVSRNPVDLKGFIGQHGDV
                     IVKPLDGMGGQSIFRVQAGDPNTNAIIEAVSKYGRSQTMAQRFIPEISDGDKRILLID
                     GKPIPYALARLPAAGENRGNIAAGASTRGQPLSERDHWLCAQIGPDLRARGLLFVGID
                     VIGDYVTEINVTSPTGVRELDREFGINISAELFECIEARLSG"
     gene            1833..>2067
                     /locus_tag="VIW27_02115"
     CDS             1833..>2067
                     /locus_tag="VIW27_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010444906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5738486.1"
                     /translation="MVIDDSKTIRRSAETLLKKVGCEVVTAIDGFEALAKITEHKPDI
                     IFVDIMMPRLDGYQTCALIKNNQSFKGTPVIMLS"
BASE COUNT          416 a          585 c          646 g          420 t
ORIGIN      
        1 agccgaccgc atagcccttg gcgatgccgc cgagatcgat ctgcaggtcg ggatggcggc
       61 taagcgcacg cccgccgtcg ataaccaggt cgtgcatgcc gggtaaatcc cgcttgagcg
      121 cgcgaattgc ggcggggtcc ggcggcgcgc cgtgaaaacc gtaggcggcg atcagtttgc
      181 cgagcgcggg attgaatagc ccgccgctcg agtcgtagta acctcggctg agccgtaaca
      241 gtggctccag gctgtcgggc agcggcgccg acccggacct gcgcagcgcg tcgttgaagc
      301 gggtcaggtc gctgtcttcc caggcgtgcc attgcccgcg ctgttgccgc aggcggatct
      361 cgatatcgtt taacaggcct tcggcgcggc tgaggtcatc ggtgaccagc gaaatctgga
      421 tcagcgtgcc gaattcaagc aggtgctgct cgacgagctg ctggcggggt tcgcagccca
      481 gcagcagcgc gatcggcagc agcaacagcc gcgttagccg gacagcctgg cttcgatgca
      541 ctcgaacagt tctgcgctga tgttgatgcc gaactcgcga tcgagctcgc gcaccccggt
      601 cggactggtg acgttgatct cggtgacgta atcgccgatc acgtcgatac caacgaacag
      661 cagcccgcgg gcgcgcaggt cgggaccgat ctgtgcgcac agccagtggt cgcgctcact
      721 gagcggctga ccgcgggtac tcgcgccggc ggcaatattg ccgcggttct caccggccgc
      781 cggcagccgc gccagcgcgt aagggatcgg cttgccatcg atcagcagaa tgcgcttgtc
      841 gccgtcgctg atttcgggga taaagcgctg cgccatggtt tgcgaacgcc cgtacttcga
      901 taccgcttcg atgatcgcgt tggtgttcgg atccccggcc tgcacgcgaa aaatcgactg
      961 cccgcccatg ccgtcgagcg gcttgacgat gacgtcgccg tgctgcccga tgaaaccctt
     1021 gagatcgacg gggttgcggc tgaccagggt ttcgacgcag cattgcggaa aataccgagt
     1081 gtagagtttc tcgttgcagt cgcgcaggct ggcgggtcga ttgaccacca gcacgccgtt
     1141 ttcctcggcc tgttcgagta tgtaggtgca gtagacgtac tcgagatcaa agggcgggtc
     1201 cttgcgcatc aggatggcat cgagctcgtc gagacggcaa tcgccgtcat cggtgtagtc
     1261 gaaccagtgg tcggcatcgt cgtgcgcttc gacgatgcgg aatcgcccgc gggcgatgcc
     1321 gttatcgagg cggacgtcgt cgagcgtgaa ataatggatc tgccagccgc gcttgcgggc
     1381 ttccagcatc atcgccatgg ttgtatcctt ccaggacttg atggcttcga tcggatccat
     1441 gacgatgccg agtttgcgtt gcgagggcat gagtaagtgt tcgcgttcgg ttctggcggt
     1501 tagcgggatt ctaatgaatt cggccgggca agcacatcaa attctgccgg ccgaaaccac
     1561 tgcaggccgc accgggcctt gttaaaaggt gcaatttgaa ctacttttga ggggcgcgcg
     1621 gtttcgttaa tccgacagtg aaataatggc tccctaagtg tagaatctac atgacatagt
     1681 catattaaac tactggttta acttgaaata gttcctataa tcatagcttt ataaggtggg
     1741 aaaacccacc aatactcccc gggggttacg tgagcaccga gtcacaaata gcggatggtg
     1801 agcaacagac ggtcaatttg aacgtcaagg tcatggtcat cgatgacagc aaaaccattc
     1861 gccgcagtgc cgaaaccctg ttgaaaaaag tcggttgcga ggtggtcacc gcgatcgacg
     1921 gcttcgaagc gctggcaaag attaccgagc acaagccgga cattattttc gtcgatatca
     1981 tgatgccgcg cctggatggc taccagacct gtgcgctgat caagaataac cagagcttca
     2041 agggcacgcc ggtcatcatg ctgtcca
//
LOCUS       DATCZV010000093         6847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_694755, whole genome shotgun sequence.
ACCESSION   DATCZV010000093 DATCZV010000000
VERSION     DATCZV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694755"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1292)
                     /locus_tag="VIW27_02120"
     CDS             complement(3..1292)
                     /locus_tag="VIW27_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5738487.1"
                     /translation="MKKALLLVGSALVFSLTQLGANAAGDEENIKKLSSFKSTGGQRG
                     EVIEQDTKFAANVRANIIPNIKLPTGFKIELFAVAPDARHMAVSRNKGAVWIGTRKTR
                     VWQATDRDMDNVADTVEQFAPTVSFDIPNGPCYSADGHLYIAERNRVLWFPAAEYFME
                     SPDTVAIPIIDQGELIPPEEESYNHTARICAIGPDNKLYVSMGQPFNVAGPDKYPLYD
                     EVGIGGMIRFNRFPGELEREVVAIGIRNSVGHAFNPQDGSLWFTDNQVDGMGDETPPG
                     ELNKACGLGSNVWYGHPYYGGGEVRTNEYSGQTIPAAYADNYCKPQVEMAAHAADLGM
                     MFYTGMMFPEKYRGAIFSAQHGSWNAVKPRGARIMVTYLDADGNASKTEPFAEGWLTE
                     MGVYLGRPVDVQQYVDGSLLVSDDKAGVVYRISYEGS"
     gene            1562..2149
                     /locus_tag="VIW27_02125"
     CDS             1562..2149
                     /locus_tag="VIW27_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738488.1"
                     /translation="MKYAETIRRLITGPLLPIACLAALHSFAVAASSDGLSVAAECRN
                     GVPEGKYQIESAQGRVRVKGSFVDGLRDGEFVFYTANGDRMIVLPYTRGLLNGAVKAW
                     HVGAGDANSPLKLLSDIKDGFIKGRHRTWYENGKPRSDFVIEDGEITAGETWNPDGTV
                     LEIKDRAQFLSAEIQSDFAYYERLEQVMDAHPPTC"
     gene            2165..2632
                     /gene="ybaK"
                     /locus_tag="VIW27_02130"
     CDS             2165..2632
                     /gene="ybaK"
                     /locus_tag="VIW27_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY5738489.1"
                     /translation="MTPGIEQARRAGIEYRVHQYEHDPASESYGLEAAQKTGVEEARV
                     FKTLVVELATGDLAVGVVPVAAMLNLKLVARAAGARKAAMAQPADVERSSGYVLGGVS
                     PLGQKKRLATFIDESANGFATIFVSAGRRGVEIELAPGDLARLTDGEFAALCR"
     gene            2766..3149
                     /locus_tag="VIW27_02135"
     CDS             2766..3149
                     /locus_tag="VIW27_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011574315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5738490.1"
                     /translation="MPQDLDANRQNAIEFYRTAFLGDPLAAVERYVGAEYIQHNPEVA
                     DGKAGFIDYFTEMARDYPHKQIEFVRAVAEGDLVALHTHQTWPGGDEYVTMDFLRFDD
                     DGKIVEHWDAIQQVPTATRCGNPMY"
     gene            3367..3639
                     /locus_tag="VIW27_02140"
     CDS             3367..3639
                     /locus_tag="VIW27_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY5738491.1"
                     /translation="MDWKVFATIFGAVFIAELGDKTQLATMLFAADRTVSKYLVFAAA
                     SAALVVASAIGVLAGSLLSEYIDERYLHYIAGIGFIAIGIWTLYNA"
     gene            3687..4301
                     /locus_tag="VIW27_02145"
     CDS             3687..4301
                     /locus_tag="VIW27_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5738492.1"
                     /translation="MQLYDLARSGNCYKVRLLLSILGVDYEKIPVDANAGDLQTAEFL
                     AINPNGLVPVLIDGDKTIYDSAAILIYLALTHGGEDWISSAPLELGSIVRWLTFEQAE
                     GRYGLARARAIKLQLRTPLVASGSYEESKMLAESALKTYQQRLSEHEWLAGGNRPTIA
                     DIACYPYTALITDAGIALEAYTAIGRWLQAIENLPGYIALPRVS"
     gene            4342..4500
                     /locus_tag="VIW27_02150"
     CDS             4342..4500
                     /locus_tag="VIW27_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3012 domain-containing protein"
                     /protein_id="HEY5738493.1"
                     /translation="MKKLWLLLLVFALAGCEPEIGSDAWCAQLKEKPRGEWTLHETGD
                     FAKHCIFN"
     gene            4785..5021
                     /locus_tag="VIW27_02155"
     CDS             4785..5021
                     /locus_tag="VIW27_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5062 family protein"
                     /protein_id="HEY5738494.1"
                     /translation="MKPLKNEKEMVKKAIAEGIKYGEQRGVVEFEPTDSAVEKIEYIY
                     RLLVHDGVIQPLPQEQISQKAMKHRIAIWASKLD"
     gene            complement(5142..5585)
                     /locus_tag="VIW27_02160"
     CDS             complement(5142..5585)
                     /locus_tag="VIW27_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY5738495.1"
                     /translation="MFDSLKHWLDTLKQESRLFTHADEEVLHAALASLLFHVISADQQ
                     VGDREKREFTRIMKQEFDLNAEQIEHLFQAASASAGDPQADLRTIAAHLKRNPSVRMS
                     FMKKLLQLIDVDGTRSDELKLFYDALREIFPGVSEVARDYDPRAD"
     gene            complement(5691..>6847)
                     /locus_tag="VIW27_02165"
     CDS             complement(5691..>6847)
                     /locus_tag="VIW27_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5738496.1"
                     /translation="RRPRLEVTRSLEPHLALGVPQRVSLRLENHGRRHLRCWLTDFPP
                     PQLQMEGLPQRLVVEPGSGVRVRYRVTPLARGPAEFGRVGCRVLSPWRLWEKNYYYGA
                     PEQTRVYPNYKPLFRSSFVGSDQLYTDLGLQLRQRRGEGTDFHQLRDFRIGDSLRQID
                     WRATARFQKPISREYQEERDQQVVFLLDCGRRMRARDGEISHFDHALNALLISAFVAL
                     RQGDSVGLLSFAGQSRWVAPIKGRFQISRLLDQIYDLDSSPVTSDFLDVAQQVMSRQT
                     RRSLIVLISSLEPQDRDDLVSAARLLSQRHLVMVASMRQQILTDTLESEVVSIDDALR
                     YCGVQQHLQQQAAMYAELRSDNIIVADTPPAHMHSTLINEYMSLKRSGVF"
BASE COUNT         1357 a         2134 c         2083 g         1273 t
ORIGIN      
        1 gatcaactgc cttcgtagga gatgcggtaa acgacgccag ccttgtcgtc ggaaaccagc
       61 aacgagccgt cgacatactg ctgcacgtcg accgggcggc ccaggtagac gcccatctcg
      121 gtcaaccagc cctcggcgaa tggctcggtc ttggaggcat taccatcggc gtcgagataa
      181 gtcaccatga tgcgggcgcc acgcggcttg accgcattcc acgaaccatg ctgggcgctg
      241 aagatcgcac cgcgatactt ctccgggaac atcataccgg tatagaacat catgccgaga
      301 tcggcggcgt gcgcggccat ttccacctgc ggcttgcagt agttgtccgc gtaagccgcc
      361 ggaatcgtct gcccactgta ctcgttggta cgcacttcgc caccgccgta gtacggatgt
      421 ccataccaga cattcgagcc gaggccgcaa gccttgttca gctcgccggg cggggtttcg
      481 tcacccatgc catcgacctg gttgtcggtg aaccacagcg aaccgtcctg cggattgaat
      541 gcatggccga ccgagttgcg aataccgatc gcgacgacct cgcgctccag ttcacccggg
      601 aagcggttga agcggatcat gccgccgata ccgacctcgt cgtaaagcgg gtacttgtcc
      661 gggccggcga cattgaacgg ctgccccatg ctgacataga gcttgttgtc gggaccgatc
      721 gcgcaaattc gtgcggtgtg gttgtaagac tcttcctccg gcggaatcag ttcgccctgg
      781 tcgatgatcg ggatcgccac cgtgtccgga ctttccatga agtactcggc cgccgggaac
      841 cacaatacgc ggttacgctc ggcgatgtac aaatggccgt cggccgaata gcaaggcccg
      901 ttgggaatgt cgaagctgac cgtcggcgcg aactgttcga ccgtatcggc gacgttgtcc
      961 atgtcgcgat cggtcgcctg ccagacccgg gttttgcggg taccgatcca caccgcgccc
     1021 ttgttgcggc tcaccgccat gtggcgagca tccggcgcca ccgcgaacaa ctcgatcttg
     1081 aatccggtcg gcagcttgat attcggtatg atattggcgc gaacattggc ggcgaacttg
     1141 gtgtcctgct cgatcacctc accgcgctgc ccgccggtac tcttgaaact gctcagcttc
     1201 ttgatgtttt cctcgtcacc agccgcgttg gcgcccagct gggtcaacga aaataccagt
     1261 gccgatccga ctaacaatag tgcttttttc atcacgtgct cctcctttgt aattatggac
     1321 tgccgattta atcatagttt ttgcaaagcc caaagcaaat tgaacaggca tcagcaaatc
     1381 cgcacggctt cgcgggaacc aaccgtgccg gcgatacggc cgacaaaatc ggcaagtgcg
     1441 acgcccccgc ttcccgtcac accggcctcg ccgccgttaa aatcttgcgt tttctcggtt
     1501 atattgtcgt ctcgccggac cgctaattcg caacccaacc agttcgcaaa cgatcaaaat
     1561 tatgaaatat gcagaaacaa ttcgccggct gataaccggc ccactgctgc caatcgcttg
     1621 cctggcggcg ctgcattcat tcgcggtcgc ggcgagcagc gacggtttga gcgtcgcggc
     1681 cgagtgccgc aatggcgttc ccgagggcaa ataccagatc gaatcggctc aggggcgcgt
     1741 gcgcgtcaag ggaagcttcg tcgacggctt gcgcgacggc gagttcgtgt tttacaccgc
     1801 gaacggcgac aggatgatcg tgctgccata caccaggggc ctgctcaatg gcgcggtcaa
     1861 ggcatggcat gtcggtgccg gcgatgcgaa ttccccgctc aagctgctct ccgacatcaa
     1921 ggacggcttc atcaaggggc gtcaccggac ctggtacgaa aatggcaagc cgcgttcaga
     1981 tttcgtcatc gaggacggtg aaatcaccgc cggagaaacc tggaacccgg acggcacggt
     2041 tctcgagatc aaagatcgtg cccagttcct gagtgccgag attcagagtg atttcgccta
     2101 ttacgagcgc ctcgagcagg tcatggacgc tcatccgccg acctgttgag gcagcaccgg
     2161 tggcatgact ccgggtatcg aacaggcacg gcgcgccggg atcgagtacc gggtgcacca
     2221 gtacgagcat gacccggcca gtgaatccta cggcctcgaa gcggcgcaaa agaccggggt
     2281 ggaagaggca cgggttttca agacactggt tgtcgaactg gcaaccgggg atctcgccgt
     2341 cggcgtggtg ccggtagcgg cgatgctgaa cctgaagcta gtcgcccgcg cggccggtgc
     2401 gcgcaaggcg gcgatggcac agcccgccga cgtcgaacgc tccagtggtt acgtgctcgg
     2461 cggcgtcagc ccgctgggcc agaaaaagcg actggcgaca ttcatcgatg aatcggccaa
     2521 cgggttcgcg acgattttcg tcagcgccgg ccggcgcggc gtcgagatcg agctggcgcc
     2581 gggcgacctg gcgcgtctga ccgacggcga attcgccgcg ttgtgccgtt agcgatacga
     2641 tcccaccagc gtgcactcga agcacgcggc gcttgaaaac cccgctggcg ggccacgata
     2701 ataacgcctg atcgcaaccc cggttcggcg ccgcggcgct gcctttactc gaacggagca
     2761 cggccatgcc gcaagatctc gacgccaacc ggcaaaacgc catcgaattc taccgcactg
     2821 cttttctcgg cgacccgctc gcggcagtcg agcgttatgt cggcgccgag tatattcagc
     2881 acaatcccga ggtcgccgac ggcaaggcgg gtttcatcga ttacttcacc gaaatggccc
     2941 gcgattaccc gcacaagcag atcgaattcg tgcgcgcagt cgccgagggc gacctggtgg
     3001 cgctacatac tcaccagacc tggcccgggg gcgacgagta cgtaaccatg gatttcctgc
     3061 gcttcgacga cgacggcaag atcgtcgagc actgggacgc gatccagcag gtacccacgg
     3121 caacccgatg cggtaatccg atgtactaaa ataccggctg ccacaaggct gttgtcagcg
     3181 cgagcctcca cgcctacagt cgcgcggcga tagcaggtat catgcacgcc gacaaggcgt
     3241 cgctggcgct gttagacggc gattgcccac tacaggttat cgtcggcatc gagacaccgc
     3301 ataccctgac ccggtgcggc gccgtcgact gccgcacctg aaatgagact agcggagata
     3361 aaaagcatgg actggaaagt gttcgcgacg attttcggcg cggtgttcat cgccgaactg
     3421 ggcgacaaga cgcagctggc gacgatgctg ttcgccgccg atcgcacagt cagcaagtac
     3481 ctggtgttcg ccgccgcatc ggccgcgctg gtggtcgcct cggcgatcgg cgtgctcgcc
     3541 ggcagcctgc tatccgaata tatcgacgag cgctacctgc actacatcgc cggcatcggc
     3601 ttcatcgcca tcggcatctg gacactttac aatgcctgaa aagcgcgccg gcgatatgtc
     3661 ggcatcggcc acgaggatcc atcgccatgc aactctacga cctggcccgc tcgggtaact
     3721 gttacaaggt tcggctgttg ctgtcgattc tcggcgtcga ctacgaaaag atccctgtcg
     3781 atgccaacgc cggagacctg cagaccgcgg agtttctggc catcaatccc aacggcctgg
     3841 tgccggtact gatcgacggc gataaaacca tttacgactc cgccgcgatc ctgatctacc
     3901 tcgcgctgac ccacggcggc gaggactgga tttcgtccgc tccgctcgaa ctcggcagca
     3961 tcgtccgctg gctgaccttc gagcaggccg agggtcgtta cggcctcgcg cgggcgcgcg
     4021 cgatcaagtt gcaactgcgc acgccgctgg tcgcctcggg cagctacgag gagagtaaaa
     4081 tgctcgccga gtcggcgctg aagacttacc agcagcgact ttccgaacac gaatggctgg
     4141 ccggcggcaa ccggccgacg atcgccgata tcgcctgtta cccctatacc gcgctgataa
     4201 ccgatgccgg catcgcgctc gaagcctaca ccgcgatcgg gcgctggctg caggccatcg
     4261 aaaacctgcc cggctacatc gccctgcccc gcgtatcctg atacttgcct atactcccag
     4321 ctcgaaggtc gaggtccggg tatgaaaaaa ttatggctac tattgctggt gttcgcgctg
     4381 gcgggctgtg agcccgagat cggcagcgat gcgtggtgcg cgcaactgaa agaaaaaccg
     4441 cgcggcgaat ggaccctgca cgaaaccggc gatttcgcga agcactgtat cttcaactag
     4501 cccgcgaatc ccgcgagccc ggtgagcgcc accgcaaaac ggaaagctcg acgccgtgca
     4561 agccgctggc agaagcgccg ttgagatccg cgatgcgcgt gcgttagtga gtcaggtcag
     4621 acgcgatatt cgattgccgg cgggtttgat gccgggttcg tcgattcccg tttacgggcg
     4681 tatactcgag gttcgggggt gcggtggtcg acggccgcag aatcctcgat cgggccgcgc
     4741 ggcgagtgcg gaccgttctc gaaccggcga cagggggcaa tggcatgaaa ccgctcaaga
     4801 acgaaaagga aatggtcaaa aaggcgatcg ccgagggcat caagtacggc gagcagcgcg
     4861 gcgtcgtcga attcgagccg accgactcgg cggtcgagaa aattgagtat atctatcgcc
     4921 tgctggtcca cgacggcgtc attcagccgc tgccgcagga gcagatatcg caaaaagcga
     4981 tgaagcaccg catcgccatc tgggcttcaa aactggattg ataccgcggt tgcgcgaagg
     5041 cgctgtcgac ccgcgagtat tcagtgccat cacaggcctg gcgcgcgaac tcgattatgc
     5101 cgccaatatc agtggcagcc tgccgcttcg gctcgcgcca gtcagtctgc ccgcggatcg
     5161 tagtcgcgcg ccacttcgct taccccgggg aagatttcgc gcaaggcatc gtagaacagc
     5221 ttcagctcat cgctgcgggt gccatccacg tcgatcaact gtaacagttt tttcatgaag
     5281 ctcatgcgca ccgacggatt gcgcttgaga tgagcggcga tggtgcgcag atcggcctgc
     5341 ggatcgccgg ccgaggcact ggccgcctgg aagaggtgct cgatctgctc cgcattgaga
     5401 tcgaattcct gcttcatgat tctggtgaac tcacgttttt cgcggtcccc gacctgctgg
     5461 tcggcgctga ttacatggaa aagcagcgat gccagtgccg cgtgcaggac ttcctcgtcg
     5521 gcatgcgtga acaaccggct ttcctgcttc agagtatcga gccagtgctt gagggaatcg
     5581 aacatggtat caatcgtatc aggttgcaaa cttcaaccgg cgtcgtcgcc agttgctatc
     5641 aatactatag cggcccgcca cggctgtata tttgatatgc atcaagcggg ctagaagacg
     5701 ccgctgcgct tcagtgacat gtactcgttt atcagtgtcg agtgcatatg ggccggtggc
     5761 gtatcggcaa cgatgatatt gtcgctacgc agttccgcgt acatcgccgc ctgttgctgc
     5821 aggtgctgct gtacgccgca ataacgcaac gcatcgtcga tcgacaccac ctcggattcg
     5881 agcgtatcgg tgagaatttg ctggcgcatc gaggccacca tcaccaggtg gcgttgcgac
     5941 agcagccgcg ccgcgctcac caggtcgtcg cgatcctgcg gctccagcga cgagatcagc
     6001 acgatcagtg agcgacgcgt ttgccgcgac atcacctgct gcgcgacatc gagaaaatca
     6061 ctggtcaccg gcgagctgtc cagatcgtag atctggtcga gcaggcggct gatctggaag
     6121 cgccccttga tcggcgcgac ccagcgtgac tggccggcga aactgagcag gccgacgctg
     6181 tcgccctggc gcagcgcgac gaaggccgag atcagcagcg cgttcagcgc gtgatcgaaa
     6241 tggctgatct cgccgtcgcg ggcgcgcatg cggcggccgc agtcgagcag gaacacgacc
     6301 tgctggtcgc gctcttcctg gtactcccgc gagatcggct tctgaaagcg cgcggtcgcc
     6361 cgccagtcga tctggcgcag cgaatcgccg atgcggaaat cgcgcagctg gtggaaatcc
     6421 gtgccctcgc cgcggcgctg gcgcaattgc aggccgaggt cggtgtacag ctggtcgctg
     6481 ccgacgaacg acgagcggaa cagcggcttg taattcgggt aaacccgcgt ttgctcgggt
     6541 gcgccgtagt agtaattctt ttcccataat cgccacggcg acaacacccg gcaaccgacg
     6601 cggccgaact cggccgggcc acgcgccagc ggcgtcaccc ggtagcgcac gcgcacgccg
     6661 ctgcccggct cgacgaccag ccgctgcggc aagccttcca tctgcaactg cggcggcgga
     6721 aagtcggtca gccagcagcg caggtgccgc cggccatgat tctcgagccg caggctgacc
     6781 cgctgcggca cgccgagcgc gaggtgcggt tccagcgagc gcgtgacttc caggcgcggt
     6841 cgtcgct
//
LOCUS       DATCZV010000094         2926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_641344, whole genome shotgun sequence.
ACCESSION   DATCZV010000094 DATCZV010000000
VERSION     DATCZV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641344"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..950
                     /locus_tag="VIW27_02170"
     CDS             138..950
                     /locus_tag="VIW27_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010439461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5738497.1"
                     /translation="MVKNTLVAAALTVIGGGQAWSAETSIIVQSTTSTANSGLYDHLL
                     PMFKRETGITVNVVAVGTGQALKNARNCDGDLLLVHAKSAEQKFVSEGYGVERHDVMY
                     NDFIFVGPPADPASIAGSKSALDVLARISSSQSKFASRGDNSGTHKKEMALWKEASID
                     PTQGSGKWYLETGSGMGATLNAAVGSGAYTMTDRATWISFRNKQDFEIQLEGDDALFN
                     QYGIIMVNPEKCDQVKREASQRFIDWILSDSGQAAIAAYQMNGQRLFFPNAR"
     gene            complement(973..1875)
                     /locus_tag="VIW27_02175"
     CDS             complement(973..1875)
                     /locus_tag="VIW27_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5738498.1"
                     /translation="MPPLTEYLTTRELAELLRIKERKVYDLVASGAVPCTRATGKLLF
                     PRDAIDDWLAQNETHPAAASPRADVPRPLVIVGSHDPLLEWALRASCCGLASYFDSSL
                     DGLSRFANREGIAAGLHLYDAPSGSWNNAQVESRFARGGVVLVEWAKRSRGIIVPPEA
                     VQEYAGLGDLVGKTMVPRQAEAGSQILLEALMREQQVDASGINWSPPTRSESDAVIEV
                     VEGRAAATFGLASLAAQHRLGYVPVIDERYDILVDRRAWFEPTWQSLLRFCQTTAFRE
                     HAAALAGYTIDDQFRVHFNAADLG"
     gene            complement(2036..2764)
                     /locus_tag="VIW27_02180"
     CDS             complement(2036..2764)
                     /locus_tag="VIW27_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEY5738499.1"
                     /translation="MQITAIVERLGADDFLFVDSGQAIELLSALARAPIGDDQTFLDS
                     WGHLEQDQYMADGGKYRKRRHAVYAIQNPGEPAQRMPYQPHYQTVDYNPLNGGVARYF
                     APILDDLHQSLTLAALLEFGNRVFSQLSGNHQWHIELHQFRIEARDGQLGKPTPEGVH
                     RDGVDFVIVVMIKRVNIDSGATTIFDLDNNLVGEFMLRETFDMVLVNDRRVYHGVTPI
                     TLLDPESEAFRDVLVITFKAPDNV"
BASE COUNT          604 a          907 c          891 g          524 t
ORIGIN      
        1 gtctgctcga gtatcaaacc accagcacgt ttttcgattc tccgtcgagc tcacaggccc
       61 gggcttatct cgacggcaag atcgtcattt agtcgatcgc aacgcaacca gtaagacagg
      121 agaaaatagt gaagcagctg gtcaaaaata ccctggtagc cgcagcgctt accgttattg
      181 gcggcggcca ggcctggtcg gccgaaacct cgattatcgt ccagtccacg acgtcgaccg
      241 ctaactcggg tctctacgac cacctgctgc cgatgttcaa gcgcgagacc ggcatcaccg
      301 tcaacgtggt tgcggtcgga accgggcagg ctttgaaaaa cgcccgcaac tgcgacggcg
      361 acttgctgct ggtacacgcc aaatccgcgg aacaaaaatt tgtctccgag ggttacggtg
      421 tcgagcgtca tgacgtcatg tataacgatt tcattttcgt cggaccgcct gccgatcccg
      481 cctcgatcgc cgggagcaag tccgcgctcg acgtcctggc ccgcatctcc agcagccagt
      541 cgaaatttgc ctcgcgcggc gataactcgg gcacacacaa aaaagagatg gcgctatgga
      601 aagaagcctc tatcgatccg acccagggaa gcggcaaatg gtatctcgag accggttcgg
      661 gcatgggcgc gacgctcaac gccgcggtcg gcagcggcgc ctacaccatg accgaccggg
      721 caacctggat cagcttcagg aacaagcagg atttcgagat ccagctcgag ggcgacgacg
      781 cgctgttcaa tcaatacggc atcatcatgg tcaacccgga aaaatgcgac caggtaaagc
      841 gcgaggcgtc acagcgcttt atcgactgga tcctgtcgga cagcggacag gcagcgatcg
      901 cggcctacca gatgaacggt caacgactgt ttttccccaa cgccaggtag cgcgagactg
      961 cgagtaagcc gactatccca ggtctgccgc gttgaaatgc acccggaact ggtcgtcgat
     1021 agtgtatccg gccagggccg ccgcgtgctc gcgaaacgcg gtcgtctggc aaaaccgcag
     1081 tagcgactgc caggtcggct cgaaccatgc gcgccgatcg accagtatgt cgtagcgttc
     1141 gtcgatcacc ggcacgtaac cgaggcgatg ctgggcggcc agcgaagcga ggccgaaggt
     1201 ggcggcggcg cgaccttcga cgacttcgat aaccgcatcg gattcgctgc gcgtcggcgg
     1261 cgaccaattg ataccggacg catcgacctg ctgctcccgc atcagcgcct cgagcaagat
     1321 ctgactgccg gcctcggctt gccgcggcac catggtcttg ccgaccaggt cgcctaatcc
     1381 cgcgtactcc tgcaccgcct ctggtggcac gataatgccg cgcgaacgct ttgcccactc
     1441 caccagcacg acaccgccac gggcaaaacg cgactcgacc tgcgcgttgt tccagctgcc
     1501 gctcggggca tcgtagagat gcagcccggc cgcgatgccc tcgcgattag caaaacgcga
     1561 caggccgtcg aggctactgt cgaaatagct ggccaggccg caacaggagg ctcgcagcgc
     1621 ccattccagc agcgggtcat ggctgccgac gatcaccagc ggccgcggca cgtcggcacg
     1681 cggcgacgca gcggcaggat gcgtttcgtt ctgcgccagc cagtcatcga ttgcgtcgcg
     1741 cggaaacagc agcttgccgg tggcacgggt acagggtacc gctcccgagg ccaccaggtc
     1801 gtagaccttg cgctccttga ttcgcagcag ttcggcgagt tccctggtgg taagatattc
     1861 agtcaacggc ggcacggcgt tcgagggtga cgggccaatt tagcacagtc gccgcggccg
     1921 caaaagcagg tggaaacatg cccgggccgc ggtgaacgcg tatacccgaa caaagagcct
     1981 ggtttgcgac cgttgctgcc ttaaccgcat tcccgcagtc cccggggaaa ctttgtcaga
     2041 cgttatccgg cgccttgaag gtgatcacga gcacatcgcg gaaagcctcc gactccggat
     2101 cgagcagggt gatcggggtg acgccgtgat agacgcgacg atcgttgacc agcaccatat
     2161 cgaaggtctc gcgcagcatg aattcgccca ccaggttgtt gtcgagatcg aagatcgtgg
     2221 tcgcgccgct atcgatgttg acacgcttga tcatgacgac gatgacaaaa tcgacgccgt
     2281 cgcgatgcac gccttcgggg gtcggcttgc ccagctggcc gtcgcgcgcc tcgatgcgga
     2341 actggtgcag ctcgatatgc cactggtgat tgccgctgag ctggctgaaa acccggttac
     2401 cgaattcgag cagcgcggcc agcgtcaggc tttggtgcag gtcgtccaga atcggcgcga
     2461 agtagcgcgc cacgccgcca ttcaacggat tgtaatcgac ggtctggtag tgcggctgat
     2521 acggcattcg ctgtgccggc tcgcccgggt tctggattgc gtagaccgcg tgacggcgct
     2581 tgcggtactt gccgccatcg gccatgtact ggtcctgctc gagatgtccc cagctatcga
     2641 ggaaggtctg gtcgtcaccg atcggcgcgc gcgccagcgc cgacagcaac tcgatggcct
     2701 ggccggagtc gacgaacaga aaatcatccg cgcccaggcg ttcgacgata gcggtaattt
     2761 gcaaaggatc ggcctctagc ctgtcagctt tgacaatcgt cgactggccg accgcgcggc
     2821 gcaacggctg tcatcgtaaa aaacgcgtgg ttacgaaacg atcgtaattt tccagcggct
     2881 cgccgagctg tccgtgagtc gcaagaaagt cggcgaggtc gctgat
//
LOCUS       DATCZV010000095         5109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_589031, whole genome shotgun sequence.
ACCESSION   DATCZV010000095 DATCZV010000000
VERSION     DATCZV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589031"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VIW27_02185"
     CDS             <1..427
                     /locus_tag="VIW27_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY5738500.1"
                     /translation="IDKPAPEFALKMLHAPERTLSTRDLLGQVWVLNVWASWCVSCRA
                     EHEVVTALANQNLVTVVGLNYKDESKDAVRWLQQFGDPYATSLIDYDGRVGIDWGVYG
                     VPETFVIGADGKIKYKHIGPMTMESLNQKVLPVIRELLS"
     gene            427..882
                     /locus_tag="VIW27_02190"
     CDS             427..882
                     /locus_tag="VIW27_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY5738501.1"
                     /translation="MRAWWVLLFSLLAVPAQAAIEAYEFDSAEMEADYNQLINELRCL
                     VCQNQNLSGSDADLARDLRRETYEMLQDGKSPDQVVDFMVARYGDFVLYRPQFQSNTY
                     LLWLGPFLLLLVVLYLFVRRLRARQVPVEVDEAALDEARRLLEQDEPRQ"
     gene            879..2114
                     /gene="ccmI"
                     /locus_tag="VIW27_02195"
     CDS             879..2114
                     /gene="ccmI"
                     /locus_tag="VIW27_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY5738502.1"
                     /translation="MIFWIATLALLVVALLILIVPLMRSAQSPEDDQRQRQNVQIARE
                     QKRQLDAQLQAGELDQAGYDGAYAELQKMLALELEHAEGQRAQARGKWMASVVLLAIP
                     VASLSLYFLFGEYRVIENPLLAQANPHQGQTRVEAPQMTMDEMHESIKARLQQNPEDV
                     EGWFMLGRTLMAKQQYGEAVVAYRRSDELMPNEPGILFALADAMAMNSNGNLLGEPEA
                     LIKRGLEAAPMYPNGLWLAGMAAEQRGDYSAAHRYWTRLLPLIADNASSSHEVAALIA
                     RLEEQDPSLAQAGAGSDAAVAAALSVAVDISPELKSRARPDDTVFVYAKAMQGPPMPL
                     AVKRLQLKDLPVELTLSDADAMMPAMKLSSFDQVVVGARVSPSGNPVAQSGDFFTERG
                     GIDRANPPAQISLTIDQVK"
     gene            complement(2118..2468)
                     /locus_tag="VIW27_02200"
     CDS             complement(2118..2468)
                     /locus_tag="VIW27_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5738503.1"
                     /translation="MIKSADELIRQAQLNIECVDAATARRIRDDNPDALIIDVREANS
                     VAESKLEESIHISRGVLEYGIHKVCPEANTVIITHCAGGGRASLAAHTLKQMGYHQVY
                     AICAPYEEIKAAFE"
     gene            2522..3436
                     /locus_tag="VIW27_02205"
     CDS             2522..3436
                     /locus_tag="VIW27_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5738504.1"
                     /translation="MNGQRKPSRRPALHSSAERRQRVVPLAVQFAGSALNALSLLHTE
                     WAARLLTRYWFTTFKSKPKPWVADFWNSADRRIPIKVEDTVIDVHCWGQGPLVVLMHG
                     WSGSGSQYRGFVEPLAGAGFTAVSFDAPEHGANSGQRTHMLRFSASLLAIQRQLGAIH
                     CVVAHSLGAMATAYTLQLGLQLERLVCVAPQLDSRHLFDGYRQLLHLRPALAQRSIEL
                     IEERLRELLQRDDPWAILQPAALLGERRLPGLLVFDHDDPEIPPQQFREAAVGWPDCR
                     VLETRGLGHNRILRDRGVIDAVVGYLRG"
     gene            complement(3437..4342)
                     /gene="gluQRS"
                     /locus_tag="VIW27_02210"
     CDS             complement(3437..4342)
                     /gene="gluQRS"
                     /locus_tag="VIW27_02210"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY5738505.1"
                     /translation="MPTTGRFAPTPSGPLHFGSLVAALASYCQARANGGRWLLRIEDV
                     DTPRVVAGAERQILSALEGFGFTWDGPLLYQSERFDAYRAHLEQLLADGHCFACQCSR
                     RSLREQGVASGVLGQIYPGNCRDRGLPVEAHSIRLDTRGAGRIAFHDGLYGDFSLDLE
                     REVGDFVLRRADGVFAYHLAVVVDDQQQGVDEVVRGADLLENTCLHIYLQRRLGFATP
                     RYLHLPLVNNIQGVKLSKQTGARAIDIGNAPRLLVAALDHLNQRPVEELQTSTPDEIL
                     RWAIEHWTPAAIPARPAASAESLDD"
     gene            complement(4342..>5109)
                     /gene="dapE"
                     /locus_tag="VIW27_02215"
     CDS             complement(4342..>5109)
                     /gene="dapE"
                     /locus_tag="VIW27_02215"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY5738506.1"
                     /translation="HRGSIALLITSDEEGVAVDGTVKVIETLEARGEKIDWALVGEPS
                     STERVGDVIKNGRRGSIGARLVIHGVQGHVAYPHKVDNPIHRAMPALHELARHRWDEG
                     NAFFPPTSLQISNINAGTGAHNVVPGHAEVLFNLRYSTEIDDRHIIDTTRAILDKHEL
                     DYDIEWQISGYPFLTPAGELVAAARHAIRRHAGIDAELATTGGTSDGRFIAPTGAQVL
                     ELGPVNESIHKIDEHVDVEQLDTLSEIYQAILRELLA"
BASE COUNT          877 a         1560 c         1738 g          934 t
ORIGIN      
        1 gatcgacaag ccggcgcctg agtttgcgct gaagatgctg catgcgccgg agcggactct
       61 gtccaccagg gacctgctcg gccaggtctg ggtgctcaac gtctgggcct cgtggtgcgt
      121 atcctgccgc gccgagcacg aggtggtcac cgcgctggcc aatcagaacc tggtcaccgt
      181 ggtcgggctg aattacaagg acgagtcgaa ggacgcggtg cgctggctgc agcagttcgg
      241 cgacccctac gccacctccc tgattgacta cgacgggcgc gtcggtatcg actggggcgt
      301 ctatggagtg cccgagacct ttgtcatcgg cgccgacggt aaaatcaagt acaagcatat
      361 cggaccgatg acgatggaat cgctgaacca gaaagtattg ccggtaatca gggagttgtt
      421 gagctgatgc gcgcctggtg ggtcctgcta ttcagtctgc tcgcggttcc ggcacaggcc
      481 gcgatcgagg cttacgaatt cgactcggcg gagatggaag cggattacaa ccagctcatc
      541 aacgaactgc gttgcctggt gtgccagaat cagaacctgt ccggctccga tgccgacctc
      601 gcccgcgacc tgcgccgcga aacctacgag atgttgcagg atggcaagtc gcccgaccag
      661 gtggtcgatt tcatggttgc gcgttacggc gatttcgtgc tctaccggcc gcaattccaa
      721 tccaacacct acctgctgtg gctgggcccg tttctgctgt tgctggtggt gctttatctc
      781 ttcgtgcgcc ggctgcgtgc gcgtcaggtg ccggtcgagg tcgacgaagc ggcgctggac
      841 gaggccaggc gcttactcga acaggacgaa ccacggcaat gattttctgg atagctacgc
      901 tcgcgctgct ggtagtggcg ctgctgattt tgatcgtccc gctgatgcgc agcgcgcaat
      961 cgccagagga tgaccagcgc cagcggcaaa acgtgcagat cgcacgcgag cagaaacgcc
     1021 agctcgatgc gcagctgcaa gcgggagaac tcgaccaggc aggctacgac ggcgcctatg
     1081 ccgagttgca gaagatgctc gctctcgaac tcgagcacgc cgaaggtcag cgcgcgcagg
     1141 cgcgcggcaa gtggatggcg tcggtggtgc tgctcgcgat accggtcgca agcctgtcgc
     1201 tttacttcct gttcggtgaa taccgggtaa tcgaaaaccc gctgctggcg caggccaacc
     1261 cgcatcaggg acagacccgc gtcgaagcgc cacagatgac gatggacgaa atgcacgagt
     1321 cgatcaaggc gagactgcag cagaacccgg aagacgtcga aggctggttc atgctcggcc
     1381 ggacattgat ggccaagcag cagtatggcg aggcggtggt ggcctatcgg cgcagcgacg
     1441 aactgatgcc gaacgagcca ggtatcctgt tcgcgttggc ggacgcgatg gcgatgaaca
     1501 gtaacggcaa cctgctgggc gagcccgagg cgctgatcaa gcgcggtctc gaggctgcgc
     1561 cgatgtatcc gaacggcctg tggcttgccg gcatggcggc cgagcagcgc ggggattatt
     1621 ccgccgcgca ccgctactgg actcgcttac tgccgctgat cgcggacaat gcctcgtcga
     1681 gccacgaggt cgcggctttg atcgccaggc tagaggagca ggatccgtcg ctggcgcagg
     1741 ccggcgcggg cagcgatgca gccgttgccg cggcgctgtc ggtagcggtc gacatcagtc
     1801 cggagctgaa gtcgcgcgcc agacccgatg acaccgtgtt cgtttacgcc aaggcgatgc
     1861 aggggccgcc gatgccgctg gcggtcaagc gcctgcaact gaaagatctg ccggtggaac
     1921 tcaccctcag cgatgcggac gcgatgatgc cggcgatgaa gctgtcgtcc ttcgatcagg
     1981 tcgtggtcgg tgcgcgggta tcgccgagcg gcaacccggt agcccagagc ggtgattttt
     2041 tcaccgagcg tggaggcatc gacagggcga atccgccagc gcagatatcg ctgaccatcg
     2101 accaggtcaa gtagcgatca ttcgaaggcg gccttgattt cctcgtaggg cgcgcagatc
     2161 gcgtaaacct ggtggtagcc catctgcttg agcgtgtgcg cggccagcga ggcgcgaccg
     2221 ccgcccgcgc agtgggtgat gatcaccgtg tttgcctccg gacatacctt gtgaatcccg
     2281 tattcgagta cgccgcggga aatgtgaatc gattcctcga gcttggattc cgccacgctg
     2341 ttcgcctcgc gtacgtcgat gatcaacgcg tccggattgt cgtcgcgaat tctccgcgcg
     2401 gtcgcggcgt cgacgcattc gatgttcagc tgtgcctggc gaatcagttc gtcggctgat
     2461 ttgatcatgt cttcgtgccc tgtgttggta aggagatggc cgtgcgatat tctactatca
     2521 aatgaacggg cagcgtaaac catcgcggcg gccggcgctg cacagttccg ccgagcgccg
     2581 ccagcgggtc gtgccgctgg cggtgcagtt cgccggcagc gcgctcaacg cgctgtcgct
     2641 gctgcacacc gaatgggcgg cccgcctgct gactcgctac tggttcacga ctttcaaaag
     2701 caaaccgaag ccgtgggtgg cggatttctg gaacagcgcc gatcgccgca tcccgataaa
     2761 ggtcgaggat acggtgatcg acgtgcattg ctgggggcag gggccgttgg tggtactgat
     2821 gcacggctgg agcggcagcg gttcgcagta ccgtggcttt gtcgagccgc tcgccggggc
     2881 cgggtttacc gcggtcagct tcgacgctcc ggaacacggt gcgaattccg gccagcgcac
     2941 ccacatgctg cgtttcagcg ccagcctgct ggcgattcag cggcagcttg gcgccatcca
     3001 ctgcgtcgtc gcgcattcgc tcggcgcgat ggcgacggcc tatacgctgc agcttggtct
     3061 ccaactcgaa cgcctggtct gcgtcgcgcc gcagctcgac tcgcggcatt tattcgacgg
     3121 ctatcggcag ctgttgcacc tgcggccggc gctggcacag cgctcgatcg aactgatcga
     3181 ggagcgtctg cgcgagctgc tgcagcgcga cgatccgtgg gcaatcctgc agccggcggc
     3241 gctgctgggt gagcgacggc tgcccgggct gctggtgttc gatcacgacg atcccgagat
     3301 accgccgcag caattccgcg aggcggctgt cggctggccc gattgccgag tgctggaaac
     3361 gcggggtctc gggcacaatc gcatacttcg cgatcgcggc gtgatcgacg cggtcgtcgg
     3421 ctacctgcgc ggctgatcaa tcgtcgaggc tttcggcgct cgccgccggg cgcgcgggaa
     3481 tcgcggccgg cgtccagtgc tcgatcgccc agcgaagaat ctcgtccggg gtcgaagtct
     3541 gcaattcctc taccggtcgc tggttgaggt gatcgagggc ggcgaccagc aatcgcggcg
     3601 cgttgccgat atcgatcgcg cgtgcgccgg tttgcttgct gagctttacc ccttgaatat
     3661 tgttgaccaa cggcaggtgc aggtaacgcg gggtggcgaa gccgagccgc cgctgcaggt
     3721 agatgtgcag gcaggtattt tccagcaggt cggcaccgcg caccacttcg tcgaccccct
     3781 gctgctggtc gtcgacgacg accgcgaggt ggtaggcaaa cacgccgtcg gcgcggcgca
     3841 gcacgaaatc accgacctcg cgctcgagat cgagcgagaa atcgccgtag aggccgtcgt
     3901 gaaaggcgat tctgccggcg ccgcgggtgt cgaggcgtat cgagtgggct tccacgggca
     3961 ggcccctgtc gcggcaattg ccggggtaga tctggcccag cacaccgctg gcgacaccct
     4021 gctcgcgcag gctgcggcgg ctgcactggc aagcgaaaca gtggccgtcg gcgagcaatt
     4081 gctcgaggtg cgcgcggtaa gcatcgaaac gctcgctctg gtacagcagc gggccgtccc
     4141 aggtaaagcc gaagccctcc agcgcgctca ggatctggcg ctcggcgccg gcgaccacgc
     4201 gcggggtatc gacgtcctcg atgcgcagca accagcggcc gccgttggcg cgcgcctggc
     4261 aatagctggc cagtgccgcg accagtgatc cgaagtgcag cggacccgag ggcgtgggcg
     4321 cgaagcgacc ggtggtcggc atcaggcgag cagttcccgg agtatcgcct ggtagatttc
     4381 cgacagggtg tcgagctgct cgacgtcgac gtgctcgtcg attttgtgga tcgactcgtt
     4441 gaccggcccg agctcgagta cctgcgcgcc ggtgggagcg atgaatcgac cgtccgaggt
     4501 gccgccggtg gtagccagct cggcgtcgat accggcgtgg cggcgaatcg catgccgcgc
     4561 ggccgcgacc agttcacccg ccggcgtcag gaacgggtag ccggagatct gccactcgat
     4621 gtcgtagtcg agctcgtgct tgtcgaggat cgcgcgcgtg gtatcgatga tatgccggtc
     4681 gtcgatctcg gtcgaatagc gcaggttgaa cagtacctcg gcatgaccgg gcaccacgtt
     4741 gtgcgcgccg gtgccggcgt tgatattgga aatctgcagg ctggtcggcg gaaagaacgc
     4801 attaccctcg tcccagcgat ggcgcgcgag ttcgtgcaac gccggcatcg cgcggtgaat
     4861 cggattgtcg accttgtgcg ggtaggcgac gtgaccctgc acgccgtgaa tcaccaggcg
     4921 cgcgccgatc gagccgcggc ggccgttctt gatcacgtcg ccgacgcgct cggtgctcga
     4981 cggttcgccg accagcgccc agtcgatctt ttcgccgcgc gcctcgaggg tttcgatgac
     5041 cttgacggtg ccgtcgacag cgacgccttc ctcgtcgctg gtgatcagca gggcgatcga
     5101 gccgcggtg
//
LOCUS       DATCZV010000096         3846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_747490, whole genome shotgun sequence.
ACCESSION   DATCZV010000096 DATCZV010000000
VERSION     DATCZV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747490"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..2905)
                     /locus_tag="VIW27_02220"
     CDS             complement(35..2905)
                     /locus_tag="VIW27_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5738507.1"
                     /translation="MKRILINATQAEELRVAMVNGQQLYDLDIEVPAREQKKSNIYKG
                     KITRIEPSLEAAFVNYGAERHGFLPFKEIARQYLSDSARDDGGKLSIKDALKVDQEII
                     VQVEKEERGNKGAALTSFISLAGRFLVAMPQNPRAGGVSRRIEGEDRDELKRVLSGLK
                     MPEGMGVIVRTAGVGRSIEEMQWDLDYLTQVWTAIEKAAEEGKAPFLIYQESDIVIRA
                     LRDHYRADIGEILIDSPEAYQQAQDFMSMVMPGSLNKLKLFEDKLPLFNRFQIENQIE
                     SAFAREVNLPSGGAIVIDHTEALTSIDVNSARATRGADIEETAKNTNLEAADEIARQL
                     RIRDLGGLIVIDFIDMLQNKNQREVETRLRKAVYDDRARVQIGRISRFGLLEMSRQRL
                     RPSLEESSQIVCPRCSGQGKIRDVESLSLAVLRLLEEESLKENTGKILAKIPVECATY
                     LLNEKREKIDSLEQRRNVHIVVIPDPKLETPHYQIERVRDNDTHRHESNYKKSYELTT
                     EETPVDLVGLASSTPVIPETAAVQRITPTGPRPAEKPAGPTPQPGLLSRLLRSLFGGE
                     ASSDDREAEKSTAESRNTRDGNRGRRGKNRGGQKSRQRGTGSAAKSSSASGGDKREGA
                     ADRETPARESGDNQDSRRGQRSGRSRGGKGSANRGNRGGRQADGDAREQKADDNDSRA
                     AAKQQDDSRQETSSQSSSRRKSGSRSSGRRRGNGGKSEHTAASENNDSNKPTKANDDR
                     QPAADSTDSRGATDSDQSSGRPKQPGGRSGRGSDAKREQQANADGGGEAGNKAPAAAP
                     RDESATPAPPVSASSAKGEGGDNGNRKNPESAKREQSPAPAASDSGSSAKAEAGDTDS
                     RKPPESVQRNESPAPAVTGSGSSAKAEAGDTDSRKPPESVQRNAPSAPAVTGSGSSAN
                     ADGGDAAGAESKSPRRDEPAKSEAATKSATRTDSEATLAGQD"
     gene            3268..>3846
                     /locus_tag="VIW27_02225"
     CDS             3268..>3846
                     /locus_tag="VIW27_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5738508.1"
                     /translation="MSSIKPQKTAVRLVDITAAQGNQRLDNFLISQLDGVPRTRVYRI
                     IRKGEVRVNRKRCKPDYKLQPGDQVRIPPLLAGREKPARSSPPEALLQRLEQAILFEN
                     QHLLIIDKPAGLAVHAGSGIDYGVVDALRLLRPADDIELAHRLDRDTSGCLMLAKSRP
                     ALLALQQAIRENRVGKNYFAVVQGCWPRELTEI"
BASE COUNT          588 a         1192 c         1223 g          843 t
ORIGIN      
        1 cgatacgctc ctcaatcgct actagtttcc cgggctagtc ctggccggcc agtgttgcct
       61 cgctatcagt ccgggtggcg cttttggtag ccgcttcgga tttcgccggc tcgtctcgcc
      121 gcggcgattt ggactccgcg ccggcggcat cgccgccatc ggcattggcg ctgctgccgg
      181 agccggtcac ggctggtgcc gacggcgcgt tgcgctgcac tgactcgggg ggcttcctgc
      241 tgtcggtatc gccagcctcg gctttggcgc tgctgccgga gccggtcacg gctggtgccg
      301 gcgactcgtt gcgttgcact gactcggggg gcttcctgct gtcggtatcg ccagcctcgg
      361 ctttggcgct gctgccggag tcggatgcag ccggcgccgg cgactgctcc cgctttgctg
      421 actcggggtt tttcctgttg ccgttgtcgc cgccttcgcc cttggcactg ctggcggaga
      481 cgggcggggc tggcgtggcc gactcatccc gcggtgccgc tgccggcgcc ttgttgccgg
      541 cttcgccgcc accgtcagcg tttgcttgct gctcgcgttt ggcatcgctg ccgcgcccgc
      601 tccgaccgcc aggctgcttc ggcctgccac tggattggtc cgaatctgtg gcgccccgtg
      661 aatcagtaga atccgcggcg ggctgccggt cgtcgttggc cttggtcggc ttgttgctgt
      721 cgttgttttc gctagccgcg gtgtgctcgg acttgccgcc gttacccctg cgcctgccgg
      781 aagaccgtga gcccgatttg cgtcgcgaac tcgactggct ggatgtttcc tgcctggagt
      841 cgtcctgctg cttggcagcc gcgcgcgaat cgttatcgtc ggctttttgc tcgcgggcgt
      901 cgccatcggc ctggcggccg ccgcgattgc cgcggtttgc ggatccctta ccgccgcgcg
      961 agcgacccga acgctgcccg cgccggctgt cctggttgtc accgctctcc ctggcgggcg
     1021 tttccctgtc ggccgcgcct tcgcgcttgt cgccgccgct cgcggatgac gacttggcag
     1081 ccgagccggt accgcgctgg cggcttttct ggccgccgcg gttcttgccg cgccggccgc
     1141 gattaccgtc gcgcgtatta cggctttccg ccgtggattt ttcggcttcg cgatcgtcgc
     1201 tgctggcttc gccaccgaac agtgaacgca acagcctgct caacagtccc ggttgcgggg
     1261 tagggccggc gggcttctcc gctgggcggg ggccggtggg cgtaatgcgt tgcacggccg
     1321 cggtctccgg gatcaccggc gtactgctcg ccagtccgac caggtcgacc ggagtttcct
     1381 cggtggtcaa ttcgtaggac ttcttgtaat tcgattcgtg gcggtgggta tcgttgtcgc
     1441 gcacacgctc gatctggtaa tgcggtgtct ccagcttcgg atcgggaatg acgacgatgt
     1501 gcacattacg gcgttgctcc agcgaatcga ttttttcgcg cttttcgttg agcaggtaag
     1561 tcgcgcactc gaccgggatc ttggccagta tcttgccggt gttttccttc agcgactcct
     1621 cctcgagcag tcgcaatacc gccagtgaca gtgactcgac atcgcgaatc ttgccctggc
     1681 cgctgcagcg cgggcagacg atctggctcg attcctcgag tgacggccgc aggcgctgac
     1741 gggacatttc cagcagtccg aagcgtgaga tacggccgat ctgcacgcgc gcgcgatcgt
     1801 catagaccgc cttgcgcagg cgggtctcga cttcgcgctg gttcttgttt tgcagcatgt
     1861 cgatgaaatc gatgacgatc agcccgccga ggtcgcgaat acgcaactgg cgcgcgatct
     1921 cgtcggcggc ctcgaggttg gtattcttgg cggtttcctc gatgtcggca ccgcgcgtgg
     1981 cgcgcgccga gttgacgtcg atcgaggtca gcgcctcggt atgatcgatg acgatcgcac
     2041 cgcccgacgg caggttgacc tcgcgcgcaa acgccgattc gatctggttc tcgatctgaa
     2101 agcggttgaa cagtggcagc ttgtcctcga acagcttgag cttgttgagc gaaccgggca
     2161 tgaccatcga cataaagtct tgtgcctgct ggtaagcctc aggggaatcg atcaggattt
     2221 cgccgatatc ggcgcggtag tgatcgcgca gcgcgcggat gacgatatcc gactcctggt
     2281 agatcaggaa cggtgctttg ccttcctcgg cggccttttc gatcgcggtc cagacctggg
     2341 tcaggtaatc gaggtcccat tgcatttcct cgatgctgcg gccgacaccg gcggtacgca
     2401 cgatcacgcc catgccctcg ggcattttca acccggatag cacgcgtttc agttcgtcgc
     2461 ggtcttcgcc ctcgatgcgc ctggagacgc ccccggcgcg gggattttgc ggcatggcca
     2521 ccaggaatcg cccggcgaga ctgataaagc tggtcagcgc ggcgccctta ttgccgcgtt
     2581 cctctttctc gacctggacg ataatctcct ggtcgacttt gagcgcatcc ttgatcgaca
     2641 gcttgccgcc gtcgtcgcgc gcggagtccg acaggtactg gcgggcgatt tccttgaagg
     2701 gcagaaaacc gtgccgttcg gcgccgtagt tgacaaaagc ggcttcgagg ctcggctcga
     2761 tgcgggtgat cttacccttg tagatgttgg actttttctg ttccctcgca gggacttcga
     2821 tatccagatc gtagagttgc tggccattga ccatggccac gcgcaattct tcggcctggg
     2881 tggcgttgat tagaatacgt ttcatgtaag tatatgcctt gcttgtacga cggccgggca
     2941 ctgcgcagtt cgagctgcgg tcgcggagtt tccgcgcgct tgcgcaacgg gcaagcggcc
     3001 gccggaccgg gtagcgttaa accggctaac aggcggaaac tatcgatgag cgcaaaactg
     3061 attaaaaggc agtacatgga ccgtgggtgt attaatacca gagtagtggg ccgaatcgtc
     3121 ggcctggcct ggaaaatcct ggggaaacat ctgccagagc ggtttgctcc ccgctaaaaa
     3181 aactgttttg gtagtgtttt gcctggagcg caggcagtac aatgctactg ggtcgaatat
     3241 accacagttt aaagtcgctg ccagccattg agttcaataa aaccgcagaa aaccgccgtt
     3301 cgtctcgtcg atataaccgc ggcccagggc aaccagcgcc tcgataattt tcttatttcc
     3361 caactcgacg gtgtgccgcg cacgcgcgtc taccgaatca tcaggaaagg cgaggtgcgg
     3421 gtcaaccgca agcgctgcaa gcccgactac aagctgcaac cgggcgacca ggtgcggatt
     3481 ccgccactgc tcgccgggcg tgagaaaccc gccaggtcaa gcccacccga agcattgctg
     3541 caacgcctcg aacaggcgat cctgttcgag aaccaacacc tgctgattat cgacaagccg
     3601 gccggactcg cggtgcacgc ggggtccggt atcgattacg gggtcgtcga cgcgttgcgc
     3661 ctgttgcgcc ccgcggacga catcgagctg gcgcatcggc tcgaccggga taccagcggc
     3721 tgcctgatgc tggcgaaaag ccgcccggcg ctgctggcgc tgcagcaggc gatcagggaa
     3781 aatcgggtcg gcaaaaacta ttttgccgtg gttcagggct gctggcccag agaactgacc
     3841 gaaatc
//
LOCUS       DATCZV010000097         3296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_482991, whole genome shotgun sequence.
ACCESSION   DATCZV010000097 DATCZV010000000
VERSION     DATCZV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482991"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VIW27_02230"
     CDS             complement(<1..569)
                     /locus_tag="VIW27_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5738509.1"
                     /translation="MTIEYDQLATNPANFVPLTPLSFLQRTADIYPQREAVIYNQRRY
                     SWAQIRNRCMRIASSLAARGIGRNDTVSVLAFNTPEIFESHFSVPMAGAVLNTINTRL
                     DADTVGYIIDHGESKLFICDRQLWPVLADALQSCQRRPEIVIIDDPDAVEKPELPSDL
                     EFSYYEDLVERGDDAWQWQPPADEWQALTL"
     gene            complement(687..2990)
                     /locus_tag="VIW27_02235"
     CDS             complement(687..2990)
                     /locus_tag="VIW27_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY5738510.1"
                     /translation="MKIQKVAVIGAGTMGSGIAGQVAKAGIEVWLLDLPQDDRPNALA
                     ERGLERLRDPNQPGLVSKQAEEFIHLGNTRDDFEQLADCDWIAEAVVERLDVKRDLYR
                     RLDEVCRADAIITSNTSTIPIRLLVEEMPPAFRRRFAITHFFNPVRFMRLLELVRGED
                     TDDAVIALLADFCEQQLGKGVVPCRDTPGFLGNRVGVFAIQTALHAAFDMGLAPTEAD
                     AIFGRPMGIPKTGVFGLYDLIGIDLMSDVVQSLVDILPADDPFHAVAAKIPLMSQMIA
                     DGRTGNKQGQGFYRDTDGGREVLDLASAQYGSAPRLLLPLAERAERHGLRVLLDDDGE
                     HGRYAWRVLSHTLAYAASLIPEVTGNPVDIDDAMKLGYNWIKGPFELIDEIGVDDFIA
                     RLEKDGLAVPAFLRQAAGSSFYRIDDARLQARRGSGDWRAIERPDGVIRFNELRQTLT
                     PRNSCEVASWYDLDGTALVEFHSKANALAADSMAMLADALDAVEGGGLKGLLVHNDAQ
                     HFSCGVNLQAVRDFYRREDMDGLDGFLEDFQRTVHRMQVADFPVVAAPVGLSIGGGFE
                     VVLHAREVICHANSVTGLVESLVGVVPGGGGCKETLYRWIDKLGCGTDIAPACWKAFM
                     NLGYGRTATSPLIAREQAMLRTNDRFLINRDRILGEALQAIENPLGQAAFERPPLAMP
                     GRPLFEEMVKWLEQSRDKGLFMAHDVKVGTEMARIVTGGDIQPGTTWNEQDFYDAERR
                     SFLALVSTAATRERIDSMLDAGAPVRN"
     gene            complement(2987..>3296)
                     /locus_tag="VIW27_02240"
     CDS             complement(2987..>3296)
                     /locus_tag="VIW27_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5738511.1"
                     /translation="IAIGRQIMAANPLYADLVACSNYTRGSEVAAGIEVPSQLILARN
                     DRLTPVKAGMAIAAQLRAKVTVIERYGHMLPIEAPRQTLQKLRELIAAQEAAAEGQAA
                     "
BASE COUNT          539 a         1045 c         1092 g          620 t
ORIGIN      
        1 agcgtcagtg cctgccattc gtctgcgggt ggttgccatt gccaggcatc gtcgccgcgc
       61 tcgaccaggt cctcatagta gctgaactcg aggtcgctcg gcagctccgg tttttccacc
      121 gcgtcggggt cgtcgatgat gacgatttcc gggcgccgtt gacagctttg cagcgcatcg
      181 gcgagcaccg gccacagctg gcggtcgcag atgaacagct tcgattcgcc gtgatcgatg
      241 atatagccca cggtgtcggc gtcgagccgg gtgttgatgg tgttcaacac ggcgcccgcc
      301 atcggcaccg agaaatggct ttcgaagatt tccggggtat tgaacgccag caccgatacc
      361 gtgtcgttcc tgccgatgcc gcgcgccgcg aggctcgacg cgatgcgcat gcagcggttg
      421 cggatctgcg cccagctata gcggcgctgg ttgtaaatca ccgcttcgcg ctgcgggtag
      481 atgtccgcgg tgcgctgcag gaaagacaag ggtgtcaacg gcacgaagtt ggccggattg
      541 gttgcgagtt ggtcgtattc aatcgtcatg tcggtttcta gcctgtatct gcttcgcttt
      601 acggaacctg tcgttctgtt tggtgatccc gcatcggcgc cgcgcgcttt gcgggatcac
      661 agggccgcgc cgccggcgcg cctgaatcag ttcctgaccg gcgcgccggc gtcgagcatg
      721 ctgtcgatgc gctcgcgggt cgccgcggtc gacaccagcg ccaggaacga gcgccgctcg
      781 gcgtcgtaga aatcctgctc gttccaggtc gtgccgggct ggatatcgcc gccggtgacg
      841 atgcgcgcca tctcggtgcc gaccttgacg tcgtgcgcca tgaacaggcc cttgtcgcgc
      901 gattgctcga gccatttgac catttcttcg aacagcggtc taccgggcat cgccagtggc
      961 ggacgctcga aagccgcctg gccgagcggg ttttcgatcg cttgcagggc ttcgccgaga
     1021 atgcggtcgc ggttgatcag aaaacgatcg ttggtacgca gcatcgcctg ctcacgcgcg
     1081 atcagcggcg aggtcgcggt ccggccgtag ccgagattca tgaacgcttt ccagcaggcc
     1141 ggggcgatat ccgtaccgca gccgagcttg tcgatccagc ggtacagagt ttccttgcag
     1201 ccgccgccgc ccggcaccac gccaaccagc gattcgacca gcccggtcac cgagttggcg
     1261 tggcagatga cttccctggc atgcagcacc acctcgaagc caccgccgat cgacaggccg
     1321 accggcgccg cgaccaccgg gaagtcggcg acctgcatgc ggtgcacggt tcgctgaaag
     1381 tcctcgagga aaccgtcgag tccgtccata tcttcgcgac ggtagaaatc gcgcaccgcc
     1441 tgcaggttga cgccgcagga gaagtgttgc gcgtcgttgt gtaccagcag acccttgagc
     1501 ccaccgccct cgaccgcgtc gagggcatcg gcgagcattg ccatcgaatc ggcagcgagc
     1561 gcattggcct tgctgtgaaa ctcgaccagt gcggtgccgt cgaggtcgta ccagctggcg
     1621 acctcgcaac tgttgcgcgg cgtcagcgtc tggcgcagct cattgaagcg gatgacacca
     1681 tcgggacgct cgattgcccg ccagtcgccg ctgccgcggc gcgcctgcag gcgcgcatcg
     1741 tcgatccggt aaaagctgga gccggctgcc tggcgcagaa aggccggtac cgctagacca
     1801 tccttttcga ggcgcgcgat gaaatcgtcg acgccgatct cgtcgatcag ctcgaacgga
     1861 cccttgatcc agttgtatcc gagcttcatc gcgtcgtcga tatcgaccgg attgccggtg
     1921 acctcgggaa tcagcgacgc ggcgtaggcg agggtgtgcg acagcacgcg ccacgcgtat
     1981 cgaccgtgtt cgccgtcgtc atcgagcagc acgcgcaggc cgtgccgttc ggcgcgttcg
     2041 gcgagcggca gcagcaggcg cggcgcggag ccgtattgag cgctggcgag atccagtact
     2101 tcgcggccgc cgtcggtgtc gcggtaaaag ccctggccct gcttgttgcc ggtgcggccg
     2161 tcggcgatca tttgcgacat cagtggaatt tttgccgcca ccgcgtggaa cgggtcgtcg
     2221 gctggcagga tgtcgaccag gctttgcacc acgtcggaca tcaggtcgat gccgatcagg
     2281 tcgtagagcc cgaacacgcc ggttttcgga atccccatcg ggcggccgaa gatcgcatcg
     2341 gcctcggtgg gcgcgaggcc catgtcaaag gcggcatgca acgcggtctg gatcgcgaac
     2401 acaccgacgc ggttgccgag aaatcccggc gtgtcgcggc acggcaccac gcccttgccg
     2461 agctgctgct cgcagaaatc ggccagcagt gcgatcaccg cgtcgtcggt atcctcgccg
     2521 cgcaccagtt cgagcagccg catgaagcgc accgggttga agaagtgcgt aatcgcgaaa
     2581 cgccggcgaa atgccggcgg catttcctcg accagcagac gaatcggaat cgtcgacgtg
     2641 ttcgaggtga tgatcgcatc ggcgcggcag acttcgtcga gacgccggta aagatcgcgc
     2701 ttgacgtcga ggcgttcgac cacggcctcg gcgatccagt cgcaatcggc gagctgctcg
     2761 aaatcgtcgc gggtgttacc caggtgaata aattcctcgg cctgcttgct gaccaggccc
     2821 ggctggttcg gatcgcgcag gcgctcgagg ccgcgttcgg cgagcgcgtt gggcctgtca
     2881 tcttgcggca ggtccagcag ccacacctcg atgccggcct tggcgacctg gccggcgatg
     2941 ccgctgccca tggtgccggc gccgatgacc gcgacttttt gaattttcat gcggcttggc
     3001 cttcggcggc ggcctcttgt gcggcgatca gttcgcgcag tttttgcagc gtctggcgcg
     3061 gcgcttcgat cggcagcatg tggccgtagc gctcgatgac cgtaaccttt gctcgcagct
     3121 gcgcggcgat cgccatgccg gccttgaccg gcgtcaggcg gtcgttgcgg gcgaggatca
     3181 attgcgacgg cacctcgatg ccagccgcga cctcgctgcc gcgcgtgtaa ttgctgcagg
     3241 cgaccaggtc ggcgtaaagc ggattggcgg ccatgatctg gcggccgatg gcgatc
//
LOCUS       DATCZV010000098         4597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_218953, whole genome shotgun sequence.
ACCESSION   DATCZV010000098 DATCZV010000000
VERSION     DATCZV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218953"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VIW27_02245"
     CDS             complement(<1..1325)
                     /locus_tag="VIW27_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5738512.1"
                     /translation="MELYFLVLLIAIMVFALTSGFPVAFALPGAAVLTIGIAALCGWL
                     FEGNTDAYFILGGPNQWLSAGVTNYRSLYWVAERDTLIAIPLFIFMGIMLQRSKIAED
                     LLVAMAQLFGPIPGGLGISVVFVGALLAATTGIVGATVIAMGLISLPAMLRNNYSKSL
                     ACGTICASGTLGQIIPPSIVLIILADQLSNAADQAATARKAEYKEATGEFSMPGDFDV
                     VTASAGDMFMGAFIPGMILVGIYMAYILISALIKPANAPPVPFEGQYDRKFVVRVVLA
                     LVPPLTLIFIVLGSIILGVATVNQAGAIGAIGAMLMGSYRLMEGKPRAYWPAAMAISA
                     VVAVAILLQFFNLNIKNIESNWDAFGIFLATIAALVLLYAICWGIWRAYKIDNTLHAV
                     MLDTAKTTSMVFIILLGAAMLTSAFRGFGGEELVKEHLTALPGGFWTQF"
     gene            complement(1329..2357)
                     /locus_tag="VIW27_02250"
     CDS             complement(1329..2357)
                     /locus_tag="VIW27_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5738513.1"
                     /translation="MARIDSLLLSRAFAYSMVSAVFLYLINNYLVFWQDMPGPFILFS
                     HYQWLGYEALSNPLDDSQLAQGWVQVAVYLAVLAGSVGYVLRTPHRTLDEESIRFATL
                     SAFIVRFAFWSVLLIGIVDMLISMLRIENILEHLIGDWLTQQLGRAVFRGTWVHYPLV
                     FLSLFIALRLRTVSFTWLALMCVLAEFMIVISRFVFSYEQAYMGDVVRFWYAALFLFA
                     SAYNLVEEGHVRVDVLYAGFSRRKKAKFDAIGCLLLGMPICWVILMQGMGGRGNSINS
                     PLLSFEVSQSGYGMYVKYIMAAFLIVFAVTMLIQFVSYLLYNIEQLRKRDEHGEHPTY
                     QQLITGKS"
     gene            complement(2434..3525)
                     /locus_tag="VIW27_02255"
     CDS             complement(2434..3525)
                     /locus_tag="VIW27_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5738514.1"
                     /translation="MKRRDVLKGGVAAALATGASTLSAPAIAQNRVEITMVSTWPRDF
                     PGLGTGAQRFAESLTAMTDGRIKVNYFAAGERVKAFDSFDEVASGNAQMYHAADYYWK
                     GKHPGFAYFTGVPFGMTYTEINAWMRFGGGQELHDELAAEYGLKPLPCGNTGVQMGGW
                     FRKEMRSAADFKGLKMRMPGLGGDVIAKLGASSVTLPGGQIYENLVSGAIDATEWVGP
                     WNDSFLKFYEAAKFYYYPGMHEPGSQLTVGVNKKFWDGLSKTDQQIIAAAAEMENSIM
                     MSEYNAKNGAALASLINDQGVKLKSFSDDTYDAFGDAAAEVFAEVQQHSPMAKKIHES
                     FLKSRNEVGSWVKISDQAYVAQRNRVLGL"
     gene            complement(3641..4255)
                     /locus_tag="VIW27_02260"
     CDS             complement(3641..4255)
                     /locus_tag="VIW27_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEY5738515.1"
                     /translation="MDNSEILNRLNLHADAIVIAIVVLAVMLLVLLNHKRLRVAFREW
                     RIQRCLSKIGSEQIRQLVCDDGLDGQYRIDRLALTPKAILLINYRPYVGNIYCAERIA
                     EWTQVIGQKSFKFANPLFEIDNQLASLRLLIGNTPIRGYLFFNHSAEFPKGHPESVLT
                     PDSIPGGLLAANGDPVQPEIQAAWEQLKLHHKDAAANRQVGLKT"
     gene            4359..>4597
                     /locus_tag="VIW27_02265"
     CDS             4359..>4597
                     /locus_tag="VIW27_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5738516.1"
                     /translation="MTSPHLTVKQLFDRESCTYTYLLIDADTGEGAIIDAVLETFDRD
                     LQIINELGVELLYAIETHAHADHITSAGKLREMTGA"
BASE COUNT          958 a         1371 c         1382 g          886 t
ORIGIN      
        1 atgaactgcg tccagaatcc gcccggcagc gcggtcaaat gctccttgac cagttcttcg
       61 ccgccgaagc cgcgaaacgc cgaggtcagc atcgccgcgc cgagcaggat gatgaatacc
      121 atcgacgtgg tcttggcggt atcgagcatc accgcgtgca gcgtattgtc gatcttgtag
      181 gcccgccaga ttccccagca gatggcgtat agcagcacca gcgccgcgat cgtggcgagg
      241 aagatgccga acgcatccca attgctctcg atgttcttga tgttgagatt gaagaactgc
      301 agcaggatgg ccaccgcgac caccgcgctg attgccatcg ccgctggcca gtaggcccgc
      361 ggcttgccct ccatgagtcg atagctgccc atcagcatgg cgccgatcgc gccgatggca
      421 cccgcctggt tgacggtcgc cacgccgagg atgatcgagc ccagcacgat gaagatcagt
      481 gtcagcggtg gcaccagcgc gagcaccacg cgaaccacga acttgcggtc gtactggccc
      541 tcgaacggca ccggcggcgc gttcgcgggc ttgatcaatg ccgagatcag aatgtaggcc
      601 atgtagatgc cgaccaggat catgcccggt atgaaggcgc ccatgaacat gtcgccggcg
      661 ctggcggtga ccacgtcgaa atctcccggc atcgaaaact cgccggtggc ttccttgtac
      721 tcggccttgc gcgcggtcgc cgcctggtcg gccgcgttgg agagctggtc ggcgaggatg
      781 atcagcacga tagacggcgg aatgatttgc ccaagggtcc ccgacgcgca gatcgtgccg
      841 caggccagcg acttggagta attgttgcgc agcatcgccg gtagcgatat cagtcccatc
      901 gcgatgaccg tggcgccgac gataccggtg gtcgcggcga gcagcgcgcc gacgaatacc
      961 accgagatgc cgagcccgcc ggggatcgga ccgaacagct gcgccatcgc caccagcagg
     1021 tcctcggcga ttttcgaacg ctgcagcatg atgcccatga aaataaacag cgggatcgcg
     1081 atcaacgtgt cgcgttccgc gacccagtag agacttcggt aattggttac cccggcgcta
     1141 agccactggt tgggcccgcc gagtatgaaa taggcatcgg tattgccctc gaacagccag
     1201 ccgcacagcg cggcgatgcc gatggtcagg actgcggcgc cgggcagggc gaaggcgacc
     1261 ggaaatcccg aggtcagcgc gaacaccatg atcgcgatca gcaataccag aaagtagagt
     1321 tccatgtctt aactcttgcc ggtgatcaat tgctggtaag tcgggtgttc gccatgttcg
     1381 tcgcgtttgc gcagctgttc gatgttgtac agcaggtagc tgacgaactg gatcagcatg
     1441 gtgaccgcga acacgatcag gaaagccgcc atgatgtact tgacgtacat gccatagccg
     1501 ctttgcgaga cttcgaaact gagcagcggg ctattaatgc tgttgccgcg cccgcccatg
     1561 ccctgcatca ggatcaccca gcagatcggc atgccgagca gcaggcagcc gatggcatcg
     1621 aatttcgctt tcttgcgccg cgaaaaaccg gcgtagagca catcgacgcg cacgtggcct
     1681 tcctcgacca ggttgtaggc gctggcgaac aggaacaggg ctgcgtacca gaagcgcacc
     1741 acgtcaccca tgtaggcctg ctcgtaggag aacacgaagc gcgagatgac gatcatgaac
     1801 tcggccagca cgcacatcag agccagccag gtgaaggata ccgtgcgcag gcgcagggcg
     1861 atgaacagcg acaggaatac cagcgggtag tgaacccagg tgcccctgaa taccgcgcgg
     1921 ccgagctgtt gggtcagcca gtcgccgatc agatgctcga ggatgttttc gatgcgcagc
     1981 atcgagatca gcatgtcgac gataccgatc agcaacaccg accagaacgc gaaacgcacg
     2041 atgaacgcgg acagggtcgc gaagcggatg ctttcttcgt ccagggttcg gtgcggtgtc
     2101 cgcagtacat agccgacgct ccccgccagc accgccaggt aaaccgcgac ctgcacccag
     2161 ccctgcgcca gctggctgtc gtctagcggg ttgcttagcg cctcgtagcc gagccactgg
     2221 taatgcgaaa acaggatgaa cggccccggc atatcctgcc agaagaccag gtaattattg
     2281 atcaggtaaa gaaaaaccgc gcttaccatc gagtaagcga atgcccggct tagcagcagg
     2341 gaatcgatcc tggccattta gcgtttaata cccgatacga aataaaacgg agcccgaaga
     2401 ctccgtttca tgtggcgtcg aaccggctac cgcttacaga ccaagcacgc ggttacgctg
     2461 cgcgacgtag gcctggtcgg aaatcttgac ccatgagccg acctcgttac gcgacttgag
     2521 gaagctctcg tgaatcttct tcgccatcgg gctgtgctgc tggacctcgg caaagacttc
     2581 cgccgcggcg tcgccgaagg cgtcgtaggt gtcgtcggag aaactcttca gcttgacccc
     2641 ctggtcgttg atcaggctgg ccagcgccgc accgttcttg gcgttgtact cggacatcat
     2701 gatcgagttc tccatctccg cggcggccgc gatgatctgc tggtcggtct tgctgaggcc
     2761 gtcccagaac ttcttgttga cgccgacggt cagctgcgag cccggctcat gcatgcccgg
     2821 gtagtagtag aacttggcgg cctcgtagaa tttcaggaag ctgtcgttcc acggacccac
     2881 ccactcggtg gcgtcgattg cgccggatac caggttctcg taaatctggc cgccgggcag
     2941 ggtaaccgag gaagcgccga gcttggcgat gacgtcgccg ccgagacccg gcatgcgcat
     3001 tttcaggccc ttgaaatcgg ccgccgaacg catttccttg cggaaccagc cgcccatctg
     3061 tacgccggtg ttgccgcagg gcagcggctt gaggccgtat tccgcggcca gctcgtcgtg
     3121 cagttcctgg ccgccgccga agcgcatcca ggcgttgatc tcggtgtaag tcatgccgaa
     3181 aggcacgccg gtgaaatagg cgaagcccgg gtgcttgcct ttccagtagt aatccgccgc
     3241 gtggtacatc tgcgcgttgc cggaagcgac ctcgtcgaag gaatcgaagg ccttgacgcg
     3301 ctcgccggcg gcaaagtaat tgaccttgat gcggccgtcg gtcatcgccg tcaggctctc
     3361 tgcgaagcgc tgcgcgccgg tgccgagacc cgggaagtcg cgcggccagg tggataccat
     3421 ggtaatttcg acacggttct gggcgatggc cggcgccgag agcgtggacg ctccggttgc
     3481 gagggcggcg gccacgccac ctttgagtac atcacgacgt ttcatttttg gttatctcca
     3541 attatcaata tatttcttgg caggttaacg actgaatcag ggttcttggt tgcgacccct
     3601 tatttcaacg ccgtcattct gggctttcta cccggacatt tcaagtttta agaccgacct
     3661 ggcgattggc agcagcatct ttgtggtgca atttcagctg ttcccaggcg gcctggattt
     3721 cgggctgaac gggatcgccg ttggcggcca gcaatccccc gggaatactg tcgggggtca
     3781 acaccgattc ggggtgaccc ttgggaaatt cggcgctgtg gttgaagaac aggtaaccgc
     3841 gaatcggggt gttgccgatc aacagcctca gcgaggccag ctggttgtcg atctcgaaca
     3901 gtgggttcgc gaacttgaaa ctcttttggc cgatcacctg ggtccactcc gcgattcttt
     3961 cggcgcagta aatattgccg acgtagggac ggtagttgat cagcaggatc gccttcggcg
     4021 tcagcgccag ccggtcgata cggtactgcc cgtcgagtcc gtcgtcgcaa accagctgcc
     4081 gaatctgctc gctgccgatt ttcgacaggc agcgctgaat ccgccattcg cgaaacgcga
     4141 ctcgcaggcg cttgtggttg agcagcacca gcagcatcac tgcgagtacc acgatcgcga
     4201 tcacgatcgc atccgcatga aggttcagac ggttgagaat ttcactgtta tccatggaac
     4261 aaaataatat ccgaaggtcg gcaaaatgcc atcgacaatt gccatcgccg agggtaaaaa
     4321 gttaaacttg atcgggccat tcgctatctt gattcgagat gacttccccc cacctgaccg
     4381 taaagcagtt gttcgaccgc gagagttgca cctataccta tctgttgatc gacgccgata
     4441 ccggcgaggg tgcgataatc gacgcggtcc tcgaaacctt cgatcgcgac ctgcagatca
     4501 tcaacgaact gggcgtcgaa ttgctgtacg cgatcgagac ccacgcgcat gccgaccata
     4561 tcacctcggc cggcaaattg cgcgagatga ccggcgc
//
LOCUS       DATCZV010000099         4013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_219034, whole genome shotgun sequence.
ACCESSION   DATCZV010000099 DATCZV010000000
VERSION     DATCZV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219034"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..982
                     /locus_tag="VIW27_02270"
     CDS             173..982
                     /locus_tag="VIW27_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5738517.1"
                     /translation="MPLTTAEIETYRRDGLVIPSAYRLPPSTLARIGALYQKLLDDNR
                     DNPDFSPDFILGPHLDAGDSYGIKGDPQWLDFARIPDILDMVEQLIGADFILWGMTIF
                     GKPARVGKATPWHQDGDYYPIEPLETLTVWISLDGSTPEQGCMRYIPGSHREHRVYSH
                     HFEHRDDYTLAQVIDDGQVDLEQARDIVLEPGQISLHDVYLVHGSGANRSDRRRMGLV
                     LRIMPASSFYNHDSGKIKEDAGSPHGYSNRALFLLRGTDRSGRNDFTRGHQ"
     gene            979..1749
                     /locus_tag="VIW27_02275"
     CDS             979..1749
                     /locus_tag="VIW27_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5738518.1"
                     /translation="MNNVGFIGVGELALYTVRGWRRGGYRGRILLSPRNRETAGLLAN
                     ECACEVAVDNQAVVDDADLVVIATRPADCLAALAPLEFRAGQVLVSVVAGIDSAELRQ
                     VVPVNVEIVRAMPVSSAAAGASPTLIYPQHEFVRQLFDRCGNTIAVDSEDYFNQGSIL
                     ACVYSWFFALYGELIEATQGPRLPAELSAELVLGMAQGAARLALAEPGSSPRQIAAAI
                     ATDGTYSKLGLDLLEDREAFAAWHEACELLAQKLAANR"
     gene            1973..2335
                     /locus_tag="VIW27_02280"
     CDS             1973..2335
                     /locus_tag="VIW27_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738519.1"
                     /translation="MIQHPSFIRWHERQLFGSFAWLVSCLMCGLLFFAVIEFVALKSS
                     GIFTIITLLVLYAIGLAAIELFRRFWLNFSFAQHCAGLATCSSCDSYGLFEVRNETWP
                     IYARCQKCGHQWVIGDDD"
     gene            2410..3378
                     /locus_tag="VIW27_02285"
     CDS             2410..3378
                     /locus_tag="VIW27_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY5738520.1"
                     /translation="MPAKDTCIYLGQALADYGFKDGHPFGPQRHDAFKEELLRQGLDQ
                     RLSVQTPVAASPDRIGLFHTGDYIDKVRDYSVSGHGYLDGGDTPSFVGMFEAASHVAG
                     SVTDAVDRIMKGEFRRAFSPIAGLHHARRHVAAGFCVFNDCGIAIEYLRAQYGIRRVA
                     YIDIDAHHGDGVFYSFEDDPDLIFADIHQDGRTLYPGTGFVNETGTGAAEGTKLNVPV
                     PPFSNDAVFNEVWPIVEDYLRKQQPEFILLQCGADSIKGDPITNMEFSPAAHAHTAAR
                     LRLLADEFCDGRMLAMGGGGYNHDNIARGWTAVVEAMSRPAEKLPA"
     gene            complement(3385..3684)
                     /locus_tag="VIW27_02290"
     CDS             complement(3385..3684)
                     /locus_tag="VIW27_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEY5738521.1"
                     /translation="MLITFKTTAYADITMFGDVGKQLLKMMGCSGEVPGAIRPEDLPA
                     ALDSLEAALARTPRKVEAAGDDDDDQPRVGLHTRAGPLLELLRAAIADDAHVRWE"
     gene            complement(3687..>4013)
                     /locus_tag="VIW27_02295"
     CDS             complement(3687..>4013)
                     /locus_tag="VIW27_02295"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017399905.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY5738522.1"
                     /translation="KGTRLLGVRAVVAESFERIHRSNLIGMGVLPLQFIDGFDRKQLQ
                     LGGEETISISGMQNGLQPGHKLELSVTDRNGSVLSVDVLCRIDTRDEVAYYEAGGILQ
                     YVLDRG"
BASE COUNT          722 a         1343 c         1199 g          749 t
ORIGIN      
        1 gcatccgcgg ccaggctctg gaagacgccg cattgtaccg cccccttcga tctctggata
       61 ccttacctgt ctgcttgcgc gaaacggcag cgcgcagcgc gcaagccagg ctttgtcgca
      121 aaccagggct ggctggctgt acccgttttc ggctttgtaa tatgctctcg gcatgcccct
      181 gaccacagcc gaaatcgaaa cctaccggcg cgacggactg gtgattccgt ccgcctatcg
      241 tctgccgccc tcgacgctgg cgcgaattgg cgcgctttat cagaaattgc tcgacgataa
      301 tcgcgacaac ccggatttct cgcccgattt cattctcggc ccgcatctcg acgccggcga
      361 ctcctacggc atcaagggcg atccgcagtg gctcgatttc gcgcgtattc ccgacatcct
      421 cgacatggtc gagcaattga tcggtgccga tttcattctc tggggaatga cgatcttcgg
      481 caaaccggcg cgcgtcggca aggcgacgcc gtggcaccag gacggcgact attacccgat
      541 cgagccactg gaaacgctga ccgtgtggat atcgctcgac ggctcgaccc ccgaacaggg
      601 ctgcatgcgc tatattcccg gctcgcatcg cgagcaccgc gtctattcgc accatttcga
      661 gcatcgcgac gactataccc tcgcgcaggt gatcgacgac ggccaggtcg acctcgagca
      721 ggcccgcgat atcgtgctcg aaccggggca gatttcgctg cacgatgtct atctggtgca
      781 cggctcgggc gccaaccgct ccgaccgccg ccgcatgggc ctggtgctgc ggatcatgcc
      841 ggcgagcagt ttctacaacc acgacagcgg caagatcaag gaggatgccg gctcaccgca
      901 cggctattcg aatcgcgccc tgttcctgct gcgcggcacg gaccgcagcg gccgcaacga
      961 tttcacccgg ggtcaccaat gaataacgtc ggtttcatcg gcgtcggcga gctcgcgctg
     1021 tacacggttc gcggctggcg gcggggcggt taccgcggcc gtatcctgtt gtcgccgcgc
     1081 aaccgcgaaa cagccgggct gctggcgaac gagtgcgcct gcgaagtggc ggtcgacaac
     1141 caggcggtgg tcgacgatgc cgacctggtg gtcattgcca cgcgacccgc cgattgtctc
     1201 gccgcgctcg cgccgctcga gtttcgcgcc ggccaggtat tggtcagcgt ggtcgccggc
     1261 atcgactccg cggagttacg ccaggtagtg ccggtgaatg tcgaaatcgt gcgcgcgatg
     1321 ccggtcagca gcgccgcggc cggtgccagc ccgacgctga tctatccgca gcacgaattc
     1381 gtgcgccagc tgttcgatcg ctgcggcaat accatcgccg tcgacagcga ggattacttc
     1441 aaccagggca gcatcctcgc ctgcgtctac agctggtttt tcgcgcttta cggggaactg
     1501 atcgaggcca cccagggacc caggctgccc gccgaactga gcgccgagct ggtgctcggc
     1561 atggcccagg gcgcggcccg gctggcattg gctgaacccg gcagcagccc tcggcaaatt
     1621 gccgccgcta tcgctaccga cggcacctac tcgaagctcg ggctcgacct gctcgaggat
     1681 cgcgaggcct ttgctgcgtg gcacgaggct tgcgaactac tggcgcaaaa gctcgccgcc
     1741 aaccgctagc cagcaactgc cgacagagcc ctgcaggccg cgccgccggc gccgatggaa
     1801 gagtgccggg aagaactgac agctacgcag aacttcccgc tgagcagatc cagaatcccg
     1861 tcccgcacat gaaacccgcg tagcaggggc tgtccgggcg acaaagtttt acggcaatac
     1921 ccggtaaaca gacatataat cccagctgtc accgggaaac gggtcatagg ccatgattca
     1981 acacccctcg ttcatccgct ggcacgaacg ccagctattc ggcagtttcg cctggctggt
     2041 cagctgcctg atgtgcggcc ttttgttttt cgccgtcatc gagttcgtcg ccctgaaatc
     2101 ctccggcatc ttcacgatca tcaccttgct ggtgctgtat gcgatcgggc tcgcggcaat
     2161 cgagctgttt cgccgcttct ggctgaattt ttcctttgcc cagcattgcg cgggactcgc
     2221 cacctgcagc agttgcgaca gttacggcct gttcgaggtg cgcaacgaaa cctggccgat
     2281 ctatgcccgc tgccagaaat gcggccacca atgggtgatc ggcgacgacg actgagcaac
     2341 agcgccgcat ttgaccgacc cgggcgatat ggtctttaat gacaggtcct gtaatcccgg
     2401 aaccctcata tgcccgccaa agacacctgc atttatctcg gccaggcgct cgccgattac
     2461 ggattcaagg acggccatcc gttcggcccc cagcgccacg atgccttcaa ggaagaattg
     2521 ctccgccagg gtctcgatca gcggctcagc gtgcaaacgc cggttgccgc gagccccgat
     2581 cgcatcgggc tgtttcatac cggcgactac atcgacaagg tacgcgatta ctcggtctcc
     2641 gggcacggct acctcgatgg cggcgacacg ccgtcgttcg tcggcatgtt cgaggccgcc
     2701 agccatgtcg ccggctcggt caccgatgcc gtcgaccgca tcatgaaagg tgagttcagg
     2761 cgcgcctttt cgccgattgc cggcctgcac catgcgcgcc gccacgtcgc cgccggtttt
     2821 tgcgtattca acgattgcgg catcgccatc gagtacctgc gcgcgcagta tggcatcagg
     2881 cgagtcgctt acatcgatat cgacgcccat catggcgacg gcgtgtttta cagtttcgag
     2941 gacgatcccg acctgatctt cgccgacatt caccaggacg gccgcaccct ttatcccggc
     3001 accggcttcg tcaacgagac cggcaccggc gccgccgagg gcaccaagct caacgtaccg
     3061 gtgccgccgt tttccaacga cgcggtattc aacgaggtct ggccgatcgt cgaggattac
     3121 ctgcgcaagc agcaaccgga attcatcctg ctgcaatgcg gcgccgacag catcaagggc
     3181 gacccgatca ccaacatgga attcagcccg gccgcccacg cgcatactgc cgcgcggctg
     3241 cggttgctgg ccgacgagtt ttgcgacggc aggatgctgg cgatgggcgg cggcggttac
     3301 aaccacgaca atatcgcccg gggctggacc gcggtggtcg aggcaatgtc gcggcccgcg
     3361 gaaaaattgc cggcctgagc cgcgctactc ccagcgaacg tgcgcgtcgt cggcgatagc
     3421 cgcgcgcagc aactcgagca acgggccggc tcgcgtatgc agccccaccc tcggctgatc
     3481 gtcatcatcg tcgccggcgg cttcgacctt gcgcggtgtt ctggccagcg cggcctcgag
     3541 gctgtcgagc gcggccggca aatcctcggg cctgatcgcg ccgggcactt ccccgctgca
     3601 tcccatcatc ttcagcagct gtttgccgac atcgccgaac atggtgatat cggcataggc
     3661 ggtggtcttg aaggtgatca gcatgatcag ccccggtcga gcacgtactg caggataccg
     3721 ccggcctcgt agtaagccac ctcgtcgcgg gtatcgatac gacacagcac gtccaccgac
     3781 aacacgctgc cgttacggtc ggttaccgac agttcgagct tgtgtcccgg ctgcaggccg
     3841 ttttgcatgc cgctgatgct gatggtctcc tcgccgccga gctgcagctg cttgcggtcg
     3901 aaaccgtcga tgaactgcag tggcagcacg cccatgccga tcaggttgga acggtgaatt
     3961 cgctcgaagc tctcggcgac caccgccctg acaccgagca gccgggtacc ctt
//
LOCUS       DATCZV010000100         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_271782, whole genome shotgun sequence.
ACCESSION   DATCZV010000100 DATCZV010000000
VERSION     DATCZV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271782"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2006
                     /locus_tag="VIW27_02300"
     CDS             <1..>2006
                     /locus_tag="VIW27_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5738523.1"
                     /translation="DSGNPGVVEVGIKGLYEVRLDFMDGLRAAERAEVLAAAEEVLQA
                     NRNLCEDFVGFTEVASEDWLICGEFDLEADVEIAEVKAQILFQVQDYLAPAVANYSLE
                     EMLRKRKADGSPYRSEDIFNGPRLKTGFIVDEELSDADLRSEVRLSDIISIVMDIRGV
                     IAVRDLQVNPHSHSGPLQNKWVVKVADGARAHLRMADSRLVFYKRDMPMAARDDAVEA
                     RFDALVAEARAKLETDISDQLDLPMPLGRYRNPQAYYAFQNHLPLVYGIGEAGLPANA
                     GPERIAQARQLKAYLLFFEQLLADFLSQLGHLRELLSTAPDLLHADFRQLVDSFVDWQ
                     LIYGGAEGADENERRQIVGQLIDALGDGGHSLERRNRFLDHLIARYAEQFDELANIMY
                     SSFGVGPQVLARYKCKFLDNYPVISAERGLAYNYGLDEPADIWNSDNISGLEKRLARL
                     LGIDNPSRRNLGDVAYDIYAEIDATPGDEFRFRIRNRDDGDILLSSSRNFVTRDQARA
                     EMRRAIVYGSQPVNYRSKVGSNGKHYFNVVDDSEHILASRRQGFDDEAQLQQAIDEVI
                     EYLRVNYSEEGMYLIENILLRPEQTDDPFLPICVDANCEDCAEADPYSYRIQIILPAY
                     GVRFRDQEYRAYAERLIRAEVPAHILPKICWISKQDMAVVEKAY"
BASE COUNT          410 a          607 c          614 g          375 t
ORIGIN      
        1 ggacagcggc aacccgggcg tggtcgaggt cggcatcaag ggcctttacg aggtcaggct
       61 ggatttcatg gacggcttgc gggcagcgga gcgtgccgag gtgctggccg cggccgagga
      121 agttctgcag gccaaccgca acctgtgcga ggatttcgtc ggctttaccg aggtcgccag
      181 cgaagactgg ctcatctgtg gcgaattcga cctcgaagcc gatgtcgaga ttgccgaagt
      241 caaggcgcaa atcctgttcc aggtccagga ttacctggcg cccgcggtgg ccaattacag
      301 cctcgaggaa atgctgcgca agcgcaaggc cgacggcagt ccgtaccgca gcgaggatat
      361 attcaacggg ccgcggctga aaaccggatt catcgtcgac gaagaattga gcgacgcgga
      421 cctgcgcagc gaagtgcggc tctccgacat catcagtatc gtcatggata ttcgcggagt
      481 aatcgcggtg cgggacctac aggtcaatcc gcacagccac agcgggccgc tgcagaacaa
      541 gtgggtggtg aaggtcgccg acggcgccag ggcgcatctg cggatggcgg attcgcggct
      601 cgtgttttac aagcgcgaca tgccgatggc ggccagggac gatgcggtcg aagcccgctt
      661 cgacgcactc gtggcggagg cccgcgccaa gctcgaaacc gatatcagcg accagctcga
      721 tctgccgatg ccgctgggcc gctatcgcaa cccgcaggcc tattacgcat ttcagaatca
      781 tctgccgctg gtttatggca tcggcgaagc cggcctgccg gcaaacgccg gcccggagcg
      841 catcgcccag gcgcggcaac tgaaggctta cctgctgttt ttcgagcaac tgctagccga
      901 tttcctgtcc cagctcgggc acttgcgcga acttttatcc accgccccgg atttgctgca
      961 tgcggacttt cgccagctcg tggattcatt cgtcgactgg caattgatct acggtggcgc
     1021 tgagggcgcc gatgaaaacg agcgccggca gattgtcggg cagttgatcg atgcgctcgg
     1081 cgacggcggg cattcgctcg agcggcgcaa ccgctttctc gatcacctga tcgcgcgtta
     1141 tgccgagcaa ttcgacgagc tcgccaacat catgtattcg agcttcgggg tcgggccaca
     1201 ggtgctggcg cgctacaagt gcaaattcct cgataactac ccggtgatca gcgccgagcg
     1261 cggcctcgcc tacaactatg gcctcgacga acccgccgac atctggaaca gtgacaatat
     1321 ctccgggctc gaaaaacgcc tcgcgcggct gctcggtatc gataacccgt cgcgccgtaa
     1381 cctcggcgac gtcgcttacg atatctatgc cgaaatcgac gccacccccg gtgacgagtt
     1441 tcgttttcgg atcagaaatc gcgacgacgg cgatattctg ctttccagca gccgcaactt
     1501 cgtcactcgc gaccaggctc gcgccgaaat gagacgcgcg atcgtctatg gatcgcagcc
     1561 ggttaactac cgctccaagg tcggcagcaa cggcaagcat tacttcaatg ttgtcgacga
     1621 cagtgaacat attctcgcct cgcgccgcca gggattcgac gacgaggcgc agctgcagca
     1681 ggcgatcgac gaagtcatcg agtacctgcg ggtcaattac agcgaagagg gcatgtacct
     1741 gatcgagaat atcctgctgc gcccggagca gacggacgat ccgtttttac cgatttgcgt
     1801 cgacgccaac tgcgaggatt gtgccgaggc ggatccttac tcgtatcgaa tccagattat
     1861 tttgccggct tacggcgtgc gattccgcga ccaggaatac cgcgcctacg ccgagcgact
     1921 gattcgggcc gaagtgcccg cgcacatcct gccgaagatc tgctggatca gcaagcagga
     1981 catggcggtg gtcgaaaagg cctacc
//
LOCUS       DATCZV010000101         6403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_166217, whole genome shotgun sequence.
ACCESSION   DATCZV010000101 DATCZV010000000
VERSION     DATCZV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166217"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2640
                     /locus_tag="VIW27_02305"
     CDS             <1..2640
                     /locus_tag="VIW27_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEY5738524.1"
                     /translation="GFYYKTFMWPKSMWMKYEHFIRKASGLGVAPRENDPDRYEHTHA
                     HCDLLVVGGGVAGLCAALAAGRAGARVILADEQAEFGGLTLASRAQIDGIDAAAWIEQ
                     AVAELDSMPEVTLLPRSTVFGYHDYNFLTINQRLSDHLPLAERPPSREKLWQVRARQV
                     VLATGAAERPLTFANNDRPGIMLASAVSTYANRYAVRAGNHAVVFTNNDSAYQTALDL
                     KAAGGEVIAVVDARANSTSEIAETARNAGIQVINGSVVVDTGGGSRLSSVQVMQLSAD
                     GKSVSGARRKLACDLLAVSGGWSPVVHLNAQSGARPEWDDAAAMFRPGKTAQAQVSAG
                     AANGTLDLAGCLREGAEAGSSTASACGFDAGMQAASADAVDSEAILPLWNLPSPYPPG
                     RGPKAFIDLQNDVGTADIALAVREGYHSVELVKRYTAMGFGTDQGKLGNINGMGVLAE
                     TRGQTIAATGTTTFRPNYTPVSFGAFAGQNLGDTLFDPVRKTAMHAWHEEHGALFEDV
                     GQWKRPWYYPQHGESLHQAVNRECLAVRDSVGILDASTLGKIEIRGKDAARFLEMIYT
                     NNWLKLEVGKGRYGFMLREDGMVMDDGVTIRLAEDHFFMHTTTGGAAGVLAWMERWLQ
                     TEWPQMEVFLTSVTDHWATAAVVGPNSRKVVSAVCEGIDFAAGAFPFMASRTGSIGDV
                     ECRVNRISFSGELAYEVNVPANHGRFMWEKLMQAGEQYNITPYGTETMHVLRAEKGYV
                     IVGQDTDGSVTVDDLGLGWALSKTKADFIGKRSLSRPDTIREDRKQLVGLLTAEPNTV
                     IPEGAQLVDDPRLPAPVPMIGHVSSSYFSACCGHSIALALVKGGRGRIGETIYAPLAD
                     GRVIEATISEPVFYDKEGSKADV"
     gene            2633..3211
                     /locus_tag="VIW27_02310"
     CDS             2633..3211
                     /locus_tag="VIW27_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY5738525.1"
                     /translation="MSDLFERQSPLVQIAFGEPLGNAPRIEEKPFLGYLNLRGRASHT
                     GFTAGVLKALGVEAPTEPNTVIEAGDNRIYWLGPDEWLVVTPAGQQQQVGERLRDALE
                     GVFSSVVDNSSGLTMIHVTGDNAAALLATDCPFDLHPREFRPGQCAQTRLAKAGMTIS
                     PLADGKGFEVIIRRSFADYLLRWLQDAAVAFE"
     gene            3339..3968
                     /locus_tag="VIW27_02315"
     CDS             3339..3968
                     /locus_tag="VIW27_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY5738526.1"
                     /translation="MYYGEKLNSISHLVGATLALIGLGALLTVGVQSRDPWIISSFAV
                     FGLTLVLLYTMSTLYHSFRPPHLKKLFRVLDHVSIYLLIAGTYTPFMLVSLRDGNGFL
                     ILGIIWLLAAGGILSEVLLSGRAVKVGQMSIYLGMGWACGFDFDSLAAALPEAGLHWL
                     TAGGIAYTAGVLFYILDKMQRLDHAHGIWHLFVLAGSACHFVSVIGYVR"
     gene            4210..4401
                     /locus_tag="VIW27_02320"
     CDS             4210..4401
                     /locus_tag="VIW27_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY5738527.1"
                     /translation="MKILTLLIVVALLLTIGVIGTGIWSMAHGGEFDRKHSTQLMMAR
                     VGMQGVTLLLLVWAFLLAY"
     gene            4770..4964
                     /locus_tag="VIW27_02325"
     CDS             4770..4964
                     /locus_tag="VIW27_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738528.1"
                     /translation="MKRALTLGCLVLLLLAAAKASDKGAAAGGEISVDNMTIYQLTES
                     GLALQATIKGTRFWKDDGLN"
     gene            complement(5016..5228)
                     /locus_tag="VIW27_02330"
     CDS             complement(5016..5228)
                     /locus_tag="VIW27_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738529.1"
                     /translation="MQTLTTDFPASRSLVAKLKAVFTRRSWRGVRRKPAAAAHRRGCF
                     NPDAAACGGYNEAFVIQYWAGYNPRH"
     gene            5356..6264
                     /locus_tag="VIW27_02335"
     CDS             5356..6264
                     /locus_tag="VIW27_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738530.1"
                     /translation="MKLPPMKALPAFEAVARLGSVSKAADELCVSQGAISQQLRNLED
                     HLGRELFVRTPNSIALSDEGEAFAGVVQFALGDIAAAAERITTATSHSELTVSMGQGI
                     AIRWVMPNLADFYRQHPDVNVIIDQSVRLVSFKNDGIDAAIRFCNGEFDDLDSALLFH
                     PRMYAAASPDYLEQYGELESLARPDGHRLIDHRYPSREIRSQHVHWEDVCTDGRIDPQ
                     TEVLIFPDELQSSAAAVQGRGIALVADYLAYDEFESGRLRLACAESVPARFSYYLVWP
                     ADARANPARDAFRDWLVQRFEKFRQS"
BASE COUNT         1117 a         2034 c         2092 g         1160 t
ORIGIN      
        1 ggtttttact acaagacctt catgtggccg aagtccatgt ggatgaagta cgagcatttc
       61 attcgcaagg cttccggtct cggcgttgcg ccgcgcgaaa acgatcccga tcgttacgag
      121 catacccatg cgcattgtga cctgctggtg gtcggtggcg gcgtagccgg cctttgcgcc
      181 gcgctggcgg ccgggcgtgc cggcgcgcgc gtgatcctcg ccgacgagca ggccgagttc
      241 ggcggcctga cgctggccag cagggcacag atcgacggta tcgacgcggc cgcatggatc
      301 gagcaggcgg tcgccgagct cgacagcatg cccgaagtca cgctgctgcc gcgcagtacc
      361 gtgtttggct accacgatta caattttctg actatcaacc agcggctctc cgatcacctg
      421 ccgctggccg agcggccgcc gagccgcgaa aagctgtggc aggtcagggc acgccaggtg
      481 gtgctcgcca ccggcgccgc cgagcgcccg ctgaccttcg ccaacaacga tcggcccggc
      541 atcatgctgg cctcggcggt atcgacctat gccaaccgct acgcggtcag ggccggcaac
      601 catgcggtcg tgtttaccaa taacgacagc gcctaccaga ccgcgctgga cctgaaggct
      661 gccgggggcg aggtcatcgc ggtggtcgat gcgcgcgcga attccaccag cgagatcgcc
      721 gaaaccgcgc gcaatgcggg tatccaggtc atcaacggca gcgtcgtcgt cgataccggc
      781 ggaggcagtc gcctgagctc ggtgcaggtc atgcaactgt ccgcggatgg taagtcggta
      841 agcggcgcgc gccgcaagct ggcctgcgac ttgctggcgg tgtccggcgg ctggagtccg
      901 gtggtccatc tcaacgcgca atccggcgcg cggcccgaat gggacgacgc ggcggcgatg
      961 ttccgccccg gtaaaaccgc acaggcgcag gtttccgccg gcgccgccaa cggcaccctc
     1021 gacctggcgg gttgcctgcg cgagggtgcc gaggccggta gcagcactgc cagtgcctgc
     1081 ggtttcgatg ccggcatgca ggcggcgagc gcggacgcgg tcgacagcga agcgatcctg
     1141 ccgctgtgga acctgccgtc gccgtacccg cccggccgcg ggccgaaggc tttcatcgac
     1201 ctgcaaaacg acgtcggcac cgcggatatc gcgctcgccg tgcgcgaagg ctaccactcg
     1261 gtggaactgg tcaagcgcta taccgcgatg ggcttcggta ccgaccaggg caagctcggc
     1321 aacatcaacg gcatgggggt gctcgccgag acccgcgggc agactatcgc cgccaccggt
     1381 acgacgacgt tccgccccaa ctatacgccg gtcagcttcg gcgcttttgc cggccagaac
     1441 ctcggcgaca cgctgttcga tccggtgcgc aagaccgcga tgcacgcctg gcacgaggaa
     1501 cacggcgcgc tgttcgaaga cgtcggccag tggaagcgtc cgtggtacta cccgcagcac
     1561 ggtgaaagcc tgcaccaagc ggtcaaccgc gagtgcctgg cggtgcgcga cagtgtcggc
     1621 attctcgacg cctcgacgct cggcaagatc gagattcgcg gcaaggatgc ggcgcgcttc
     1681 ctggaaatga tctacaccaa caactggctg aagctcgagg tcggcaaggg ccgctatgga
     1741 ttcatgctgc gcgaggacgg catggtgatg gacgacgggg tcaccattcg cctcgccgag
     1801 gatcatttct tcatgcacac caccaccggc ggtgccgccg gcgtgctcgc ctggatggag
     1861 cgctggctac agaccgagtg gccgcaaatg gaagtcttcc tgacctcggt gaccgatcac
     1921 tgggcgaccg cggcggtggt cggccccaac agccgcaagg tggtgtcggc ggtctgcgaa
     1981 ggcatcgatt tcgccgccgg cgcgtttccg tttatggcct cgaggactgg cagcatcggc
     2041 gatgtcgagt gccgcgtgaa ccgtatcagc ttctccggcg aactcgccta cgaagtcaac
     2101 gtgccggcca accacggacg cttcatgtgg gaaaaactga tgcaggccgg cgagcaatac
     2161 aatatcacgc cctacggcac cgagaccatg catgtactgc gcgcggagaa gggctacgta
     2221 atcgtcggcc aggataccga cggctcggta accgtcgacg acctgggcct cggctgggcg
     2281 ctgtcgaaga ccaaggccga tttcatcggc aagcgctcgc tgagccgccc ggatacgatt
     2341 cgcgaggacc gcaagcagct ggttggcctg ctgaccgccg agccgaacac cgtgataccg
     2401 gaaggcgcgc aactggtcga cgatccgcgg ctgcccgcgc cggtgccgat gatcggccac
     2461 gtcagctcga gttacttcag cgcctgctgc gggcattcga tcgcgctggc gctggtcaag
     2521 ggcggtcgcg gccgcatcgg cgaaacgatt tacgcgccgc tcgccgatgg ccgcgtgatc
     2581 gaggcgacga tcagcgagcc ggtattttac gacaaggaag ggagcaaggc cgatgtctga
     2641 cctgttcgaa cggcaaagcc cgctggtgca gatcgccttt ggcgagcctc tcggcaacgc
     2701 cccgcgtatc gaggaaaagc cgttcctcgg ttatctcaac ctgcgcgggc gagcgtcgca
     2761 tacgggcttt accgccggtg tgctcaaggc gctcggcgtc gaggcgccga ccgagccgaa
     2821 tacggtgatc gaggccggcg acaatcgtat ctactggctc gggcccgacg agtggctggt
     2881 ggtgacgccg gccgggcagc agcagcaggt cggcgagcgg ctgcgcgatg cgctcgaggg
     2941 cgtgttcagc tcggtggtcg acaattccag cggcctgacg atgatccacg tcaccggcga
     3001 caatgccgcg gccctgctgg ccaccgattg cccgttcgac ctgcatccgc gcgaattcag
     3061 gcccggccag tgtgcgcaga cgcggctggc gaaggccggc atgacgatat caccgctggc
     3121 agacggcaag ggattcgaag tcatcattcg ccgcagtttc gccgattacc tgctgcgctg
     3181 gttgcaggac gccgccgtcg ccttcgagta aaccgcgcgc gacgcgcggt cccggcctcg
     3241 attcgccagg ccgtcgacgc gcgacgcgca tggcgaaaga tggtgatccg cctttggcca
     3301 ggcgcctgaa gaaactgcct gtgtaggcga gggacgctat gtattacggt gagaagctca
     3361 atagcataag ccatctggtc ggtgccacgc tggcgctgat cgggctcggc gcgctgctga
     3421 ccgtcggcgt gcaatcgcgc gatccgtgga tcatctcgag tttcgccgtg ttcgggctga
     3481 cactggtgtt gctgtacacg atgtcgacgc tttaccacag cttccgcccg ccccacctga
     3541 agaaactgtt ccgggttctc gaccatgtgt cgatatatct gctgatcgcc ggtacctaca
     3601 cgcccttcat gctggtcagc ctgcgcgatg gcaacggctt cctgattctc ggcatcatct
     3661 ggctgctggc ggcgggcggc attctcagcg aggtattgct gagcggccgc gcggtcaagg
     3721 tcgggcagat gtcgatatat ctcggcatgg gttgggcctg cgggttcgat ttcgacagcc
     3781 tggcggcggc cctgcccgag gccggcctgc attggctgac cgcgggcggt atcgcctaca
     3841 cggccggggt attgttttac attctcgaca agatgcagcg cctcgaccat gcgcacggga
     3901 tctggcacct gttcgtgctc gccggctcgg cctgccactt cgtgtcggtg atcggttacg
     3961 tccgctaagc gggcacggcg gctgcccgct gtcggcccgc gtggtggcgc atcgttgcca
     4021 gcccgggttt cgcggccgag cgccgcgcgc agcgcgtcga ggttggccgg cggcgacgag
     4081 atgcccctcg atctcgatct gtccgcgtcc ggtcaccgcc cgctgcgcgg tggcgcatca
     4141 ttgacgcgcg tcaattcact gttgatgcgg ggggtctaag atgaatcagc aacaaaagcg
     4201 gaggtgggta tgaaaatttt gaccttgctg atcgtcgtcg ccctgttgtt gaccataggt
     4261 gtaatcggca ccggtatctg gtcgatggcg catggtggtg aattcgaccg caagcattcg
     4321 acgcagttga tgatggcgcg agtcggcatg cagggcgtca ccctgttgct gctggtgtgg
     4381 gcattcctgc tcgcctattg acgggactcg cggcgtgatg cccgcatagc tgcgcaaagc
     4441 cgtagtggcc gcgggtaagg ttagaacagg ggtccgtccc gatcttcaat gagaggacgg
     4501 agcaggatcg atatgctccg tttccggacc gactcgccca gttcgggagt caccgaaatc
     4561 accttcgttg ccgccgcctg cgttgaggcc gaacaccgcc gcgagcgggt cgctcgggct
     4621 cgcgattatc gagccgccag gagagcgcgt cgattgtctg cttcccgtgc acctcgccgg
     4681 ccgcaaatat gcaacagttc actgtaccgc ggggccgaag ccgcctatat tcccgagtcc
     4741 gaaaccaacc accttcgcag aggcatgaaa tgaagcgagc cctgaccctc ggctgcctgg
     4801 tcttgttgct gctcgccgcc gccaaggcca gcgacaaggg cgctgccgcg ggcggcgaga
     4861 tcagcgtcga caacatgacg atttaccagc tcaccgaatc cgggctggcg ctgcaggcga
     4921 cgatcaaggg tacccgcttc tggaaggacg acgggctcaa ttgaaatttc accgccgccg
     4981 ttagggtttg cgcactcggc gcaatccggt cgcgactaat gccggggatt gtaaccggcc
     5041 cagtactgga tcacgaaagc ctcgttgtag ccaccgcatg ccgctgcgtc gggattgaag
     5101 catccccggc gatgagcagc cgcggccggc tttcggcgca ccccgcgcca acttcggcgg
     5161 gtaaacaccg cctttagttt tgctaccagc gaccggctgg ccggaaaatc cgtcgtcagg
     5221 gtctgcatga tcatccctcc tgtcagaatt cgctcactgg gctcgttcag ccgtttgcgc
     5281 ttataatcgg acgcctggcg ggccctgtac atgcagaaat tcagaagggt ccattagatt
     5341 ttctggggat gccgcatgaa actaccgcca atgaaggcat tgcccgcgtt cgaggctgtt
     5401 gctcgtctcg gcagcgtcag caaggcggcc gacgagctgt gcgtcagcca gggcgcgatc
     5461 agccagcagt tgcgcaacct ggaggatcac ctcggccgcg agttgtttgt gcgcacgccg
     5521 aattcgatag ccctgtcgga tgaaggtgaa gcctttgccg gcgtggtgca gtttgcgctc
     5581 ggcgatatcg ccgcagcggc cgaacgcata accactgcca ccagccactc ggagcttacg
     5641 gtatccatgg ggcagggcat cgcgatccgg tgggtaatgc ccaacctcgc cgacttttac
     5701 cggcagcatc ccgatgtcaa cgtgatcatc gatcaaagcg tcaggctggt cagtttcaag
     5761 aacgacggca tcgacgcggc gattcgtttc tgtaacggcg aattcgacga tctcgacagc
     5821 gctttgttgt ttcatcctcg aatgtacgcg gccgccagtc ccgactatct cgagcaatac
     5881 ggcgaactcg agtcgctggc gcggcccgac ggtcatcgcc tgatcgacca ccgctaccct
     5941 agcagggaaa tcaggagcca gcatgtacac tgggaagacg tctgtaccga cgggcgcatc
     6001 gatccgcaaa ccgaagtatt gatcttcccg gacgagctgc agtcgtccgc cgcggccgtg
     6061 cagggacgcg gtatcgccct ggttgccgat tacctggcgt atgacgagtt cgagtcgggc
     6121 cggctgcggc tggcttgcgc cgaatcggtg ccggcgcgtt tcagctacta cctggtctgg
     6181 cccgcggatg cccgtgccaa cccggcgcgc gacgcgtttc gcgactggct ggtgcagcgg
     6241 ttcgaaaaat ttcgccagtc gtgacaggcc caatcgcgcg agtagcctgt caatcgccag
     6301 cctcgccggc tgccggcgcc tatcccgcga ttccggcctg cgcgatcgcg gcgcgtatat
     6361 cgtcgaccgc gtcggcggca taagcggccg gcggcagcct gcc
//
LOCUS       DATCZV010000102         4870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_537106, whole genome shotgun sequence.
ACCESSION   DATCZV010000102 DATCZV010000000
VERSION     DATCZV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537106"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="VIW27_02340"
     CDS             complement(<1..1872)
                     /locus_tag="VIW27_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5738531.1"
                     /translation="MIKVRPATLHSYWLMFVATVITVGIVTASQLITNRIGLLLDRQA
                     SELLAADMVLRSGREFSAAYGAEARRQGLDVSHSVSLRTAIFIDDDPRLVELKAVDGA
                     YPLRGRLERAAEIAGDKSVTGEIPARGEVWVDTKLAQLLGESLQLGEAGLDVEWLLTY
                     EPDRGGAIFNLAPRVLMNLDDLPDTGLVVPGSRVEYRMMFAGASQEVARFKAWLQTRL
                     EADEEIQDLENARPEMRRALDRTRKFFALAIVLTLVIAMAAIAITARYTARREAPKVA
                     MLRAFGISQVSLFGYYFRQLGVLWIAATAAGILLGWVTQYALQWALIGWFGRALPAVF
                     DWRPLVTAGLVGMIALAGFSLPYLVNATATPPMQVLRTAVSRRSWLRGGLISGSAVAA
                     VFGVLILLMQSSLLAVATLALILVIALVLPLILGSMVRLLLFSSQRRFWLRQYLLSRL
                     QSSARGAIFVMSGFSLVLIAILLIAVVKDEILGDWQQQLPADIPNYFLVNINRDDIYG
                     VAEFLEQRQIEASRPYALVRARLTQINAVDIETIDFTHPRAESMVQHTFNMTSATQLP
                     DDNRIVEGNWIDAGDNSPQLSVEQGLAERLDLEVGDELTLNIGSLVRRAPITSIRSVE
                     "
     gene            complement(1875..2555)
                     /locus_tag="VIW27_02345"
     CDS             complement(1875..2555)
                     /locus_tag="VIW27_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5738532.1"
                     /translation="MAENNLCIELNRVGKHVDTPEGRLDIVKNISLDVKYREAVVIKG
                     ASGSGKTTLLGLMAGLDQASSGEVLLFGENLAGRSEEQRSSIRAGKVGFVFQSFHLVQ
                     GATALQNVMLPLELAGADGALQTAAESLRQVGLEAKASTRVEHLSGGEQQRVAIARAF
                     AVGPRVLFADEPTGNLDAGTGRQIADLMFDLRDRFGTTLVLVTHESELLERGDRQILI
                     ESGRLVEN"
     gene            2533..3162
                     /locus_tag="VIW27_02350"
     CDS             2533..3162
                     /locus_tag="VIW27_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY5738533.1"
                     /translation="MHRLFSAIVLYVLLGFHGGAFAAHAAINILILGDSLSAAHNIPL
                     EQGWPRLFRNNIRASFEQTTVINASISGETTFGGLQRLPRLLAEHSPTHLIIELGGND
                     GLRGLDFTQTTDNLRQMIRLAKARGVVVLLIGVRMPPNFGAAYNARFQQVFEAVASEF
                     DVHYLPKFLDGVAASDPELMQDDGIHPTALAQPILANKVEQRMRQILSH"
     gene            complement(3152..3844)
                     /locus_tag="VIW27_02355"
     CDS             complement(3152..3844)
                     /locus_tag="VIW27_02355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5738534.1"
                     /translation="MFDQVSVIAFDLDDTLWPCMPTIQRAEETLYRWLQRHYPRITAR
                     HGPEELVALRREFSTREPLYAVDMTVMRRDFLRHIGSLHDYDGELLSRRGFEVFFHAR
                     QQVEFYHDVLPVLRRLKARFRLGAISNGNASVDHVGLGQLFEHAVSAADLQVAKPDPL
                     IYRHLAQRFEAAPAEILYVGDHPEYDVIGATEAGYHAVWINREQGRWPQHLPEPRHQV
                     ADLRQLEALLSG"
     gene            4011..>4870
                     /gene="ispG"
                     /locus_tag="VIW27_02360"
     CDS             4011..>4870
                     /gene="ispG"
                     /locus_tag="VIW27_02360"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEY5738535.1"
                     /translation="MINGRLSHRVDVSGIAIGGGAPVVVQSMTNTDTADIDATVAQVK
                     LLADAGSELVRITVNNDAAARAVPHIAERLEALDCRVPLIGDFHFNGHKLLAGHQACA
                     RALAKYRINPGNVGRGRKRDPQFSQMIEIACRLGKPVRIGVNWGSLDQQLLATLLTQN
                     ASLADPLPLQAINRQAVIDSALDSAAFAEHIGLPAERIVISCKMSRVPDLVEVYRRLA
                     ARCNYALHLGLTEAGMGDQGVVASSAALAILLAQGIGDTIRVSLTPEPGATREREVRI
                     AQQILQSLGIR"
BASE COUNT          909 a         1626 c         1503 g          832 t
ORIGIN      
        1 ttccacgctg cgaatgctgg taatcggcgc acgccgcacc aggctgccga tattcaacgt
       61 caattcgtcg ccgacctcga ggtcaaggcg ctcggcgagc ccctgctcca ccgacagttg
      121 cggactgttg tcgccggcat cgatccagtt gccttcgacg attcgattgt cgtcgggcaa
      181 ttgcgtcgcc gaggtcatat taaaggtatg ctgaaccatc gactcggcgc gcggatgcgt
      241 aaagtcgatg gtttcgatat cgaccgcgtt gatctgcgtc agccgcgcgc gcaccagcgc
      301 atacggacgc gaggcctcga tttgccgttg ctcgaggaac tccgcgactc cgtaaatatc
      361 gtcgcggttg atattgacga ggaaatagtt gggaatgtcg gcgggcagtt gctgctgcca
      421 gtcgccgaga atttcgtcct tgaccaccgc gatcagcagg atagcgatca ataccaggct
      481 gaaacccgac atcacgaaga ttgcgccgcg cgcactcgac tgcaggcggc tcagcaggta
      541 ctggcgcagc cagaaacggc gctgcgagga aaacagcaac agcctgacca tcgaacccag
      601 aatcaacggc agcaccagcg cgataacaag gatcagcgcc agcgtcgcga cggccagcag
      661 cgagctttgc atcaacagga tcagcacgcc gaacaccgcg gccacggcgc tgcccgaaat
      721 cagcccgccg cgcagccagg aacgccggct gacagcggtg cgcaacacct gcatcggcgg
      781 cgtcgcggtg gcgttgacca ggtaaggcag cgaaaagccg gccagcgcaa tcattccgac
      841 caggcccgcg gtcaccaacg gccgccagtc gaataccgcc ggcagcgccc tgccgaacca
      901 gccgatcaac gcccattgca gcgcgtattg cgtcacccag ccgagcagga tgccggcggc
      961 ggtggccgcg atccacagca cgccgagctg gcggaaatag taaccgaaca ggctcacctg
     1021 ggagataccg aaggcacgca gcatggcaac cttgggcgct tcccggcggg ccgtgtagcg
     1081 tgcggtaata gcgatcgccg ccatcgcgat gaccagcgtg agcacgatcg ccagcgcgaa
     1141 aaacttgcgc gtgcgatcga gcgcgcggcg catttccggg cgggcgtttt ccaggtcctg
     1201 gatttcctcg tcggcttcga gccgggtttg cagccaggcc ttgaaacgcg cgacctcctg
     1261 gctggcgccg gcgaacatca tgcggtactc gacgcggctg cccggcacca ccagcccggt
     1321 gtcgggcagg tcgtcgagat tcatcagcac ccgcggcgcg agattgaaga tcgcgccgcc
     1381 gcgatccggc tcgtaggtaa gcagccattc gacgtccaag cctgcttcac cgagttgcaa
     1441 cgactcgccg agcagttgcg ccagcttggt atcgacccat acctcgccgc gcgcgggtat
     1501 ttcgccggtc accgacttgt ccccggcgat ttcggcggcg cgttcgagcc ggccgcgcag
     1561 cgggtaggcg ccatcgacgg ctttcaattc gaccagccgc ggatcgtcgt cgataaagat
     1621 cgccgttcgc aggctgacgc tgtggctgac gtcgaggccc tgccgccggg cctccgcgcc
     1681 gtaggcggcg ctgaactcgc ggcccgaccg caaaaccatg tcggcggcca gcaattcgct
     1741 cgcctgccgg tccagcagca ggccgatgcg attggtgatc aactggctcg cggtcactat
     1801 gccgacggtt attaccgtgg cgacgaacat cagccagtag gaatgcagcg ttgccggtct
     1861 gactttaatc atcgtcaatt ctcgaccagc ctgccggact cgatcaggat ttgccggtcg
     1921 ccgcgctcca gcaattcgct ctcgtgcgtc accagcacca gcgtggtgcc gaagcggtcg
     1981 cgcagatcaa acatcaggtc ggcgatctgc cggccggtgc cggcgtcgag gttgccggtc
     2041 ggctcgtcgg cgaacagcac cctgggcccc actgcgaacg cacgcgcaat cgccacgcgc
     2101 tgctgctcgc cgcccgacag gtgctcgacg cgggtcgatg ccttggcctc cagcccgacc
     2161 tgccgcaggc tctccgccgc ggtctgcagc gcaccgtcgg cgccggcgag ttccagcggc
     2221 aacatgacgt tctgcagcgc ggtcgcaccc tgcacgaggt ggaaagactg gaaaacgaat
     2281 ccgaccttgc cggcgcgaat cgacgagcgc tgctcctcgc tgcggccggc gaggttttcg
     2341 ccgaacagca gcacttcgcc actgctggcc tggtcgagcc cggccatcag tccgagcagc
     2401 gtggtcttgc cggaccccga cgcgcccttg atgaccacgg cttcgcggta cttaacatcg
     2461 agcgagatat tctttacgat gtcgagccgg ccttccggcg tatccacgtg tttcccgacc
     2521 cggttcaact cgatgcatag attattttcg gccattgtcc tgtatgtcct gctgggattt
     2581 cacggcggcg cattcgccgc ccacgcggcc atcaacatcc tgatcctcgg cgacagcctc
     2641 agtgccgccc acaatattcc gctggaacag ggctggccgc ggttgttccg caacaatata
     2701 agggcaagtt tcgagcaaac aaccgtgatc aatgccagca tcagtggcga aaccaccttc
     2761 ggcggcctgc aacgtctgcc gcgactgctc gcagagcact cgccgaccca cctgatcatc
     2821 gagttgggcg gcaacgacgg actgcgcggg ctcgatttta cccagaccac cgacaacctg
     2881 cggcaaatga tccgcctcgc caaggcgcgc ggcgtggtcg tgttgctgat tggcgtgcgc
     2941 atgccgccca acttcggcgc cgcctacaac gcccgctttc agcaggtctt cgaagccgtc
     3001 gcctccgagt tcgatgttca ctacctgccg aagttcctcg atggcgtggc cgccagcgac
     3061 cccgagctga tgcaggacga cggcatccac ccgaccgcgc tcgcccagcc catcctggca
     3121 aacaaggtcg agcagcgcat gcggcagatt ctcagccact gagcagggcc tcgagctgcc
     3181 gcaggtcggc gacctggtgg cggggctcgg gcaggtgttg cggccaccga ccttgctcgc
     3241 gattgatcca taccgcgtgg taaccggcct cggtggcgcc gatgacgtca tactcggggt
     3301 gatcgccgac atagaggatc tcggccggtg ccgcctcgaa gcgctgcgcc aggtgccggt
     3361 aaatcagcgg gtcgggcttg gcgacctgca gatccgcggc gctgaccgca tgttcgaaca
     3421 gctgacccag gccgacatgg tcgacgctgg cattgccgtt gctgatcgcg ccgaggcgga
     3481 atctggcttt caatcgcctc agcaccggca gcacatcgtg gtaaaactcg acctgctggc
     3541 gggcatggaa aaacacctcg aaaccgcgcc gtgacaggag ttcgccgtcg tagtcgtgca
     3601 ggctgccgat atgccgcagg aaatcgcgcc gcatcacggt catgtcgacc gcgtaaagcg
     3661 gctcacgagt actgaactcg cgccgcaacg cgaccagctc ctcgggcccg tggcgcgcgg
     3721 tgatccgcgg gtaatgccgt tgcagccagc gatacagggt ttcctcggcc cgctggatgg
     3781 tcggcatgca cggccacagg gtatcgtcga gatcaaaggc gataacgcta acctggtcga
     3841 acacgtaaaa cagctcctgg caaaacggtc ccggatttta taggttttgc gccgcgcgcg
     3901 aaacaatctc cgcgacgatg accgactcgg cccgcgagcc gcgggcaagg ctttattcgc
     3961 tgacgattgt tctttactat gaccgcaacc attcgagggt ccagtaactg atgatcaacg
     4021 gcaggctcag ccatcgcgtc gacgtgtccg gaatcgccat cggtggcggt gcgccggtcg
     4081 tcgtgcaatc gatgaccaac accgataccg ccgatatcga cgccaccgtc gcgcaggtga
     4141 agctgctcgc cgacgccggc tccgaactgg tgcgcatcac ggtcaacaac gacgccgcgg
     4201 cgcgcgcggt accgcatatc gccgagcgcc tcgaggctct cgattgccgc gtgccgttga
     4261 tcggcgattt tcatttcaac ggccacaagc tgctcgccgg gcaccaagcc tgcgcgcgcg
     4321 cactggccaa gtaccgcatc aatcccggta atgtcgggcg cggccgaaag cgagatccac
     4381 agttcagcca gatgatcgag attgcctgcc gcctcggcaa gccggtgcgc atcggcgtca
     4441 actggggcag tctcgaccag caattgctgg ccacgctgct gacgcaaaat gccagcctgg
     4501 ccgacccgtt gccgctgcag gcgataaacc gccaggcggt gatcgattcg gcgctcgaca
     4561 gtgccgcgtt tgccgagcat atcggtttgc cagccgagcg catcgtgatc agctgcaaga
     4621 tgagccgggt tcccgacctg gtcgaggttt accgcaggct cgctgcgcgc tgcaactatg
     4681 cgctgcacct gggattgacc gaggccggca tgggcgacca gggcgtcgtc gcctcgagtg
     4741 ccgcgctcgc cattctgctg gcacagggaa tcggcgacac gattcgcgtc tcgctgaccc
     4801 cggaacccgg cgcgacgcgc gagcgcgagg tgcgtatcgc ccagcagata ctgcaatcac
     4861 tcggcatccg
//
LOCUS       DATCZV010000103         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_483797, whole genome shotgun sequence.
ACCESSION   DATCZV010000103 DATCZV010000000
VERSION     DATCZV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483797"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VIW27_02365"
     CDS             complement(<1..284)
                     /locus_tag="VIW27_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY5738536.1"
                     /translation="MKTLFRLLRWPLGQLVIFLDWLSGPKPPAHSPEVQQKLDAQTAS
                     LKLYQFQQCPFCVRTRRKIRGLGLNIETRDARNEPRWFAELVEQGGKYQVP"
     gene            complement(483..1826)
                     /locus_tag="VIW27_02370"
     CDS             complement(483..1826)
                     /locus_tag="VIW27_02370"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5738537.1"
                     /translation="MTDTTTTTAQMAEQVLDRVKRAGAAGDLIVDAGDTLSLKACDGE
                     LEEHKVSSSRIFGLRVIKDGRVGTAYSEAADADALGSMVDQALLNASFSRAEPFETIL
                     PNADHLATDDDLLCPDEQVSIEQKIDLALDIERRLTARDKIRNVPYNAVQDITSQRQI
                     FSSAGLCASTRSRSCAAYAYALAEDGERNAMQGSSQVSRLFSDLDASGVIDKTWDKCI
                     GLLDGKPVPSKHYDVIFDSECQVSAFRVFAMMFSGKAAKDGINPLRDKIGQSIADSRL
                     SIYDHPLNADGFGYHLFDAEGTATKKTALVVDGCLQTLIHNSATAAWADTETTGHASR
                     GPRSTLGVNLHQVEIAAGADDLPALYSGEYLELTDLTGLHSGANAISGNFSFGASGYL
                     CRDGERIQAVRGITVAGNFYEMLNKIAMIGNRQHWNEGRSALMPSIRFADVAISG"
     gene            complement(1826..3211)
                     /locus_tag="VIW27_02375"
     CDS             complement(1826..3211)
                     /locus_tag="VIW27_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016958568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY5738538.1"
                     /translation="MLNPQTAAAVIDHALSLGADFAELFVEKNAANVVSTLSDEVQSV
                     ESGINFGIGLRLVYGGKVLYGYTNRTDRNELLRIASELAAKDLRDPVSTRTAFDFNPV
                     DDLHPARKMLSADAEIDSKVAYLMAADQAARAAGEQISQTRGSCLQREQRVEIFNSDG
                     LHTADIRNYIRASLTAIATDGSEQATGSWNDGGLLGWEIEAGIDPRKTGEEAARQALV
                     NLSAKPCPSGRMPVIIGNGFGGVIFHEACGHLLETTSVAKKASVFHDQMGEMIASPIV
                     NAVDDGTLANEWGSINIDDEGMPTQRTQLIDNGRLSNFLTDRIGAQQTGLPRSGSGRR
                     ESYKFAPASRMRNTFIEAGDDSLDDMIASIDSGIYASQMGGGSVQPGTGEFNFAVTEG
                     YYIENGRIQHPVKAATLISTGPKVLKEISMIGRDFSLACGMCGSISGAVPTTVGQATL
                     KVDDILVGGNA"
BASE COUNT          609 a         1133 c         1225 g          765 t
ORIGIN      
        1 ggcacctggt acttgccgcc ctgttcgacg agctcggcga accagcgcgg ctcgttgcgg
       61 gcatcgcggg tttcgatatt caacccgagg ccgcgtatct tgcgcctggt tctgacgcag
      121 aacgggcatt gctgaaattg atagagtttc aggctggcgg tttgcgcatc gagcttttgc
      181 tgcacttccg ggctgtgtgc cggtggcttc ggcccgctga gccagtccag gaaaataacc
      241 agctggccga gcggccagcg cagcagacgg aacaaggtct tcatcaacga gcccgcatat
      301 ctcaccgatt tcctactgcg gcagtcgcaa agcgcactgt gacgcgccgt actcgcgacg
      361 gcccgcttaa cgcagtaaca actacgccgg cgcggacctc tggtccatgc ggcgcgtcac
      421 agcgtctttg cgacaatcgg tgtgccgcat cactcgctac gcaaaacggt gacatatgcg
      481 ggctagccgg agatcgccac gtcggcgaag cgaatgctcg gcatcaatgc ggagcggcct
      541 tcgttccagt gctgtcgatt gccgatcatc gcgatcttat tcagcatttc atagaagtta
      601 ccggccacgg taatgccgcg cacggcctgg atgcgctcgc cgtcgcggca caggtaaccg
      661 gaagcgccga aactgaaatt gccggagatg gcgttggcgc cggagtgcag gccggtcagg
      721 tccgtgagtt ccaggtattc gccgctatac agcgccggca ggtcgtcggc gcccgcggcg
      781 atttcgacct ggtgcaaatt gacgccgagc gtcgagcgcg gcccgcggct cgcgtgcccg
      841 gtggtttcgg tgtcggccca ggcggcggtg gcgctgttgt ggatcagggt ctgcaggcag
      901 ccgtcgacga ccagtgccgt tttcttcgtg gcggtgcctt ccgcgtcgaa caggtgatag
      961 ccgaacccgt ctgcattgag ggggtgatca taaatcgaca gccgcgaatc ggcgatgctc
     1021 tgcccgatct tgtcgcgcag cgggttgatg ccgtccttcg cggccttgcc ggaaaacatc
     1081 atcgcgaata ctctgaacgc cgacacctgg cattcgctgt cgaagatcac gtcgtagtgc
     1141 ttgctcggca caggcttgcc gtcgagcagg ccgatgcact tgtcccaggt tttatcgatc
     1201 acgccggagg catccagatc ggaaaacaat cgcgatacct ggctgctgcc ctgcatcgcg
     1261 ttgcgctcgc cgtcctcggc cagggcatag gcataggcgg cgcagctgcg gctgcgcgtc
     1321 gatgcgcata gcccggcgct cgagaagatc tgccgctggc tggtgatgtc ctgcacggcg
     1381 ttatagggta cgttccggat cttgtccctg gcggtgagcc ggcgctcgat gtcgagcgcc
     1441 aggtcgatct tctgctcgat cgacacctgc tcatcggggc acaacaggtc gtcgtccgtt
     1501 gccaggtggt cggcattggg caggatggtc tcaaacggct cggccctgga gaagctggcg
     1561 ttgagcaatg cctggtcgac catcgagccg agggcgtcgg cgtcggcggc ttcgctgtag
     1621 gccgtgccga cacggccatc cttgattacc cgcaggccga agatgcggct cgagctcacc
     1681 ttgtgttctt cgagttcgcc gtcgcacgcc ttgagcgaga gggtgtcgcc tgcatcgacg
     1741 atcaggtcgc cggccgcacc ggcgcgcttt acccggtcca gcacctgctc ggccatttgc
     1801 gcggtggtcg tggttgtgtc agtcatcagg cgttaccccc caccaggatg tcgtcgactt
     1861 tcagcgtcgc ctggcccacc gtggtcggca ccgcgccgga gatcgacccg cacatgccgc
     1921 aggccagcga aaagtcgcgg ccgatcatgg agatttcctt gagcactttg ggtccggtgc
     1981 tgatcagcgt ggccgctttc accgggtgtt ggattctgcc gttttcgatg tagtaaccct
     2041 cggtgaccgc gaagttgaat tcgccggtgc ccggttgcac cgaaccgccg cccatttgcg
     2101 aggcgtagat gccgctgtcg atcgacgcga tcatgtcgtc gaggctgtcg tcgcccgctt
     2161 cgatgaaggt gttgcgcatg cgcgacgccg gcgcgaactt gtacgattcg cggcggcccg
     2221 agccggagcg cggcaagccg gtttgctgcg cgccgatgcg gtcggtgagg aagttgctga
     2281 gcctgccgtt gtcgatcagc tgggtgcgct gcgtgggcat gccttcgtcg tcgatgttga
     2341 tcgagcccca ttcgttggcc agggtgccgt cgtccacggc gttgacgatc gggctggcga
     2401 tcatttcgcc catctggtcg tggaacaccg atgccttctt ggccaccgac gtggtttcca
     2461 gcaggtgccc gcaggcttcg tggaagatca cgccgccgaa gccgttgccg atgatcaccg
     2521 gcatgcggcc cgaagggcag ggttttgccg acaggttgac cagcgcctgc cgggcggctt
     2581 cttcaccggt tttgcgcggg tcgatgccgg cctcgatttc ccagccgagc aggccgccgt
     2641 cgttccagct gccggtcgcc tgctcgctgc cgtcggtggc gatggcggtc aggctggcgc
     2701 gaatgtaatt gcgaatgtcc gcggtgtgca ggccgtcgct gttgaagatc tcgacgcgct
     2761 gctcgcgctg caggcacgag ccacgggtct gcgagatctg ctcaccggcc gcccgcgccg
     2821 cctggtctgc cgccatcagg taggctacct tgctgtcgat ttcggcatcc gcggagagca
     2881 tcttgcgcgc cgggtgaagg tcgtcgaccg ggttgaagtc aaaggcggtt cgagtgctga
     2941 ccgggtctct caggtctttc gccgccagtt cggaggcgat gcgcagcaac tcgttccggt
     3001 ccgttcggtt ggtgtatcca tatagcacct tgccgccgta aaccagccgc aggccgatac
     3061 cgaagttgat gcccgattcc accgactgca cctcgtcgct gagggtgctg acgacattgg
     3121 cggcattctt ttcgacgaaa agctcggcga aatcggcgcc cagcgacagc gcgtgatcga
     3181 tcaccgctgc ggcggtctgg gggtttagca tcgaaatctg ttccttgctc gaggttggtt
     3241 aaagctagtc gggttggacc gcatagtggc cccacgggtt cctacatggg gttaccgcgg
     3301 gcgaatacaa taaaccattg ccgtccaccg ggatgaactc cttaccccgt cattccggcc
     3361 ttcgaggcgg aatcttgatc gcgttgctgc cccagtgagt gcaggcgaat tgacaagatc
     3421 ccggcttgcg ccgggatgac gagttggagt gagcgccggg atgacgagtt ggagagagcg
     3481 ccgggatgga agaaaaggcg gggtcatggg aaaaaaggcg aggtcgggtc ttaccattca
     3541 cattggcatt ctcgagtttg tgatgtgtaa ggcatgatcc cgatgtccag gattcaatgt
     3601 cagcggcaac tgtgcccacg ctttcgccgg caattctcga acgagcgggg atctcgagat
     3661 ggcggcgccc cggcagtcat ccccgcgcaa gcggggatct cgagatggcg gcgccccggc
     3721 agtcatcccc gc
//
LOCUS       DATCZV010000104         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_219483, whole genome shotgun sequence.
ACCESSION   DATCZV010000104 DATCZV010000000
VERSION     DATCZV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219483"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VIW27_02380"
     CDS             <1..1572
                     /locus_tag="VIW27_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5738539.1"
                     /translation="IETAEAYRKTVGEAFQSLPGLVLLYASFALHAAIALRSIYRRSS
                     LRLSPWQWAQTLLGLSVLPLLVGHAAGNRGYDLLGNIDPDYYYVVTSLLLKPVILAKL
                     VVLMLVVWIHLLIGLHFWLRIRQGYHRWLPYAYTLAVLVPTLAYAGVFNMLQQASGWL
                     DDPERLDQIYAASRAMGDREIEFLQGLESRAWMVMAALLALTLGARQLRIWIQARRGS
                     FRVNHANGSQLRELHGVSLLEALRNAGIPHASVCGGRARCTTCRVRVGNGLDALDLPN
                     ELEARALQRIGAEANVRLACQLYPHSDLSITPLVLANQDLSATLHKGGVQGHEEYVVA
                     MFVDMRGSTNLGERVLAYDVVFILNRFFTELSEALAASHGHYAQFAGDGLMALYGLDP
                     QRKPQACRDALAGAAEMFRRVEQLNDQLQSEFGESVQMGIGIHGGDAIVGTMGPPKTP
                     LLTAVGDNINIAARLEAQTKQQRCDLIVSLDTLARENIEFANAASREIELRGRENRVR
                     VCLLQRNDLPSASAD"
     gene            1628..2635
                     /locus_tag="VIW27_02385"
     CDS             1628..2635
                     /locus_tag="VIW27_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017006846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5738540.1"
                     /translation="MQQRQLGPFTVSAIGLGCMNASAGYGPRIPDDEAGALFNAALDA
                     GYTFLDTASLYAFGHNESLIGKYLAHRRDEYVLASKCGFSWTGNGENRSFDGRPEVLA
                     QTCEDSLRRLNTEVIDLYYLHRIDPQVPVEDSVGGLARLVEQGKIRTIGLSEISVGSL
                     RRAHAVHPITAVQSEYSLWSRTPEHGMLDACDELGVAFVPFSPLARQFLTGKCPDVTG
                     VLADDIRATIARPRFEPENFAKNSELLQPFGEIAARCGCSMAQLALAWLLARPGGLIP
                     IPGTKHHEWMIENAGAAEISLDADTVAELDALINEDKIAGYRYTDALMQSTDSEKDWP
                     R"
     gene            2632..>3370
                     /locus_tag="VIW27_02390"
     CDS             2632..>3370
                     /locus_tag="VIW27_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEY5738541.1"
                     /translation="MKLAAAEQSMLAGEQGEPRRWAMEHMIRVGRFFDAADFVPVTQA
                     HIMADTESLGEAGVEFLERMAAAPAADRRVRVPMITDPRGIDFCHYRRLKQTEAMAAL
                     EQRAIDAFEALGILMTNTCINYQTIMPPVRGEHLAFGDTGVVIYCNSVLGAYSNFEGG
                     PSALAAGLTGRTPRYGLHLDANRRATRRFYVTREPRDLSEWGALGGIVGARAGSYHEV
                     PVLEGIDLPPGSDELKHFGAAMASFGSV"
BASE COUNT          576 a         1140 c         1079 g          575 t
ORIGIN      
        1 atcgagaccg ccgaggctta tcgcaagacc gtcggcgagg ctttccaatc gctgccgggt
       61 ctggtgctgc tgtatgcctc gttcgcgctg catgcggcaa tcgcgctgcg ttcgatctac
      121 cggcgctcga gcctgcgcct gtcgccatgg caatgggcgc agacactgct cggcctgtcg
      181 gtgctgccgc tgctggtcgg ccatgcggcc ggcaaccgcg gttacgacct gctcggcaat
      241 atcgaccccg attactatta tgtcgtcacc tcgctgctgt tgaagccggt catcctcgcc
      301 aagctggtgg tattgatgct ggtggtctgg attcacctgt tgatcggcct gcacttctgg
      361 ttgcgaatcc gccagggata tcaccgctgg ctgccctatg cctacactct cgcggtactg
      421 gtaccgacgc tggcttatgc cggggtcttc aacatgctgc aacaggcgag cggctggcta
      481 gacgaccccg agcgcctgga tcaaatctat gccgccagcc gcgccatggg cgaccgggag
      541 atcgaattcc tgcagggtct ggaatcccgg gcatggatgg tgatggcagc actgctggcg
      601 ctgacattgg gcgcgcgcca gctgcgaatc tggattcagg cgcggcgcgg cagctttcgc
      661 gtcaaccacg ccaacggtag ccagttgcgc gagctgcacg gcgtctcgct gctcgaagca
      721 ttgcgtaacg caggcattcc gcatgcctcg gtatgcggcg gccgcgcgcg ctgcaccacc
      781 tgccgcgtac gtgtcggcaa cgggctcgac gccctcgatc tacccaacga gctggaagcg
      841 cgggccctgc agcgcatcgg tgcagaggcc aatgtgcggc tcgcctgcca gctctacccg
      901 cactcggacc tgtcgatcac gccgctggtg ctggccaacc aggacctcag cgccacgctg
      961 cacaagggcg gcgtgcaggg ccacgaggaa tacgtggtgg cgatgttcgt cgatatgcgc
     1021 ggctcgacca acctcggcga gcgggtgctc gcctacgacg tggtgttcat cctcaatcgc
     1081 ttctttaccg agctgtccga ggcgctggcc gcgtcgcacg ggcactacgc acagttcgcc
     1141 ggcgacggcc tgatggcgct ctacgggctc gatccgcagc gcaagccgca ggcctgtcgc
     1201 gatgccctcg ccggcgccgc cgaaatgttc cgccgcgtcg agcaactgaa cgatcaatta
     1261 caaagcgagt tcggcgagag tgtgcagatg gggatcggca ttcacggcgg cgacgcaatc
     1321 gtcggcacca tgggcccgcc gaaaacacca ctgttgaccg cggtcggcga caatatcaac
     1381 atcgccgcgc gcctcgaggc gcagaccaag caacagcgat gcgacctgat cgtgtcgctc
     1441 gacacgctgg cgcgtgaaaa tatcgagttc gccaatgccg ccagccggga aatcgagcta
     1501 cgcggccgcg agaaccgcgt gcgggtctgc ctgctgcagc gcaacgatct gccgtcggcc
     1561 agcgcggact gatcgaaccc cgcccgatct ttgggtatca tagtccgcca tcgacaccgg
     1621 agacatcatg cagcagcgac aattgggtcc ttttaccgta tccgccatcg gcctcggttg
     1681 catgaacgca tcggcgggtt acgggccgcg catccccgac gacgaggccg gcgcactgtt
     1741 caacgccgcg ctcgatgcgg gatacacttt cctggatacc gcctcgctgt acgccttcgg
     1801 ccacaacgag agcctgatcg gcaagtatct cgcccaccgg cgcgacgagt acgtgctcgc
     1861 cagcaagtgc ggtttttcgt ggaccgggaa cggcgagaat cgctccttcg acggccgccc
     1921 cgaggtgctc gcgcaaacct gcgaagacag cctgcggcgg ctcaacaccg aggttatcga
     1981 tctctactac ctgcatcgta tcgacccgca ggtgccggtc gaggacagcg tcggcgggct
     2041 cgcgcgcctc gtcgaacagg gcaagatcag gaccatcggc ctgtcggaga tcagcgtcgg
     2101 cagcctgcgt cgcgcgcacg cggtgcaccc gattaccgcg gtgcagtcgg agtactcgct
     2161 gtggagccgc acgccggagc acggcatgct cgacgcttgc gacgaactcg gcgtcgcctt
     2221 cgtgccgttt tcgccgctgg cgcgccaatt cctgaccggc aagtgcccgg acgtgaccgg
     2281 cgtgctcgcc gacgatattc gcgccaccat cgcgcgcccg cgtttcgagc cggaaaactt
     2341 cgccaaaaac agcgaactgc tgcaaccctt cggcgagatc gccgcgcgct gcggctgcag
     2401 catggcgcag ctggcgctcg cctggctgct cgcacggccc ggcggattga tcccgattcc
     2461 cggcaccaag caccacgagt ggatgatcga aaacgccggc gcggccgaga tctcgctgga
     2521 cgcggacacg gtcgccgagc tcgacgcgct aatcaacgag gacaagattg ccggctaccg
     2581 ctacaccgat gcgctgatgc agtccaccga ctcggaaaag gactggccgc gatgaaactc
     2641 gccgccgcgg agcaatcgat gctcgccggc gagcagggcg aaccgcgccg ctgggcgatg
     2701 gagcacatga tccgcgtcgg ccgctttttc gacgccgcgg acttcgtgcc ggtcacccag
     2761 gcgcacatca tggccgatac cgagtcgctg ggcgaggccg gcgtcgagtt cctcgagcga
     2821 atggcggcgg cgcccgcggc cgacaggcgc gtacgcgtgc cgatgatcac cgacccgcgc
     2881 ggcatcgatt tctgccatta tcggcgcctc aagcaaaccg aggcaatggc cgcgctcgag
     2941 caacgcgcga tcgatgcttt cgaggcgctg ggtattctga tgaccaatac ctgtatcaac
     3001 taccagacca tcatgccgcc ggtgcgcggc gaacacctcg cgttcggcga taccggggtg
     3061 gtgatctact gcaacagcgt gctcggcgcc tattcgaatt tcgaaggcgg tccgtcggcg
     3121 ctggccgccg gcctcaccgg gcgcacgccg cgctacggcc tgcacctcga tgccaaccgc
     3181 cgtgcgacca ggcgcttcta cgtcactcgc gagccgcgcg acctgtccga atggggcgcg
     3241 ctcggcggta tcgtcggcgc gcgggccggg tcctaccacg aggtgccggt actcgagggc
     3301 atcgacctgc cgccgggttc cgacgagttg aaacacttcg gcgcggcgat ggccagtttc
     3361 ggctcggtgc
//
LOCUS       DATCZV010000105         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_7689, whole genome shotgun sequence.
ACCESSION   DATCZV010000105 DATCZV010000000
VERSION     DATCZV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7689"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VIW27_02395"
     CDS             complement(<1..555)
                     /locus_tag="VIW27_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5738542.1"
                     /translation="MDTSLTILQVAAILIAARIFGELATWLRVPVVIGEILAGVILGP
                     SLLAFVEPQGLIQVLAEIGIILLLFQVGLETDVGELARSGSKSVVVAIGGFALPLLTC
                     FVLARYVFGLDLLVALMVAGTMTATSIGITMRTLTDLGRAHSHEGHIVLGAVVLDDIL
                     GVLLLAILFNFITLGSVDLAGTWRI"
     gene            complement(563..1183)
                     /locus_tag="VIW27_02400"
     CDS             complement(563..1183)
                     /locus_tag="VIW27_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEY5738543.1"
                     /translation="MTTRIGLVSDVHSSPGPLKQALEIFAAEGVDDIICAGDIAGYYD
                     TLEPTIDLLTTANCKTIAGNHDLSWLERPPHAGDERIRDYLQALPPTLELEIDGVRLL
                     AVHANPPAEQRGGIRLLDQHGEPRPDRQQEWTAKLSSLEHDLLIVGHTHQVYALRLGG
                     VLVVNPGSTVFNHSCMIVSLPDLTVRSFALGNRDIVACWNFGMLYR"
     gene            complement(1223..1828)
                     /locus_tag="VIW27_02405"
     CDS             complement(1223..1828)
                     /locus_tag="VIW27_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738544.1"
                     /translation="MKAIAPISTKTLVSLLLLLVLQACAGSGARIGDPDAEQARDDQL
                     GLAILSLGDDIDPDEARRAARVAIDYSHQLAREYDIVGPPLFHNTLVNLGIRDRGLCV
                     HWARDLMIRLQQERFRSLDLHWGVANYESAFRVEHNTVIISARGDALQQGLVLDPWRY
                     SGDLYWAPAHDDPDYVWRPHAEIQVLKDRRAELRQSRGVQR"
     gene            1968..>2555
                     /locus_tag="VIW27_02410"
     CDS             1968..>2555
                     /locus_tag="VIW27_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5738545.1"
                     /translation="MTSLTERLENGEILLLDGGVSTEIRRRGVALDENVWSGLTTKTH
                     PDQVREVHEDYIAAGAQVITANTYSTARHVLESINLGHEAKLLNFKSVQLAREARDDM
                     AREDVYIAGSMSSMPPLTSHREVAIDGHVESSYQELAEVLAEAGVDLIIAEMMRDIKN
                     ASIVIKAAVSTGLPVWIGYSAMLADNGVDVRSLRWK"
BASE COUNT          475 a          806 c          858 g          416 t
ORIGIN      
        1 gattcgccag gtgccggcga ggtcgaccga gcccagggta atgaagttga acaggatcgc
       61 cagcagcaac acgccgagga tgtcgtcgag caccacggcg ccgagcacga tatggccctc
      121 gtggctatgc gcacgcccga gatcggtcag cgtgcgcatg gtgatgccga tgctggtcgc
      181 ggtcatcgtg ccggccacca tcagcgcgac cagcaggtcg agcccgaaga cgtaacgggc
      241 gagcacgaag caggtcaata acggcagcgc gaagccgccg atcgcaacca ccaccgattt
      301 gctgccggac cgcgccagtt caccgacgtc ggtctccagc ccgacctgga acagcagcag
      361 gatgatgccg atctcggcca gcacctggat cagtccctgc ggctcgacga acgccagcag
      421 gctcggtccg agaatgacgc cggcgaggat ttcgccgatg accaccggaa cccgcagcca
      481 ggtggccagc tcgccgaaaa ttctggcggc aatcaggatt gcggctacct gcaggatcgt
      541 gagcgaggta tccatcgcgt ggtcagcgat acagcatgcc gaagttccag caggcgacga
      601 tgtctcggtt gccgagcgcg aagctgcgca ccgtgaggtc gggcagcgag acgatcatgc
      661 agctgtggtt gaacaccgtg ctgccgggat tgacgaccag cacgccgccg aggcgcagcg
      721 catagacctg gtgggtatga ccgacgatca gcaggtcgtg ctcgagcgac gacagttttg
      781 cggtccattc ctgttggcgg tcggggcgcg gttcgccgtg ctgatcgagc aggcgaatgc
      841 cgccacgctg ttcggccggc gggttggcgt gcacggccag cagccgcacg ccgtcgatct
      901 ccagttccag tgtcggcggc aaggcctgca ggtagtcgcg aatgcgctcg tcgccggcat
      961 gcggcgggcg ctccagccag gacaggtcgt gattgccggc gatggtcttg cagttcgccg
     1021 tcgtcagcag gtcgatggtc ggttcgaggg tgtcgtagta accggcgatg tcgccggcgc
     1081 agatgatatc gtcgacgcct tcggcggcga aaatctccag tgcctgcttg agcgggccgg
     1141 ggctcgaatg cacgtcgctg acgaggccga tgcgagtggt catgctgcgg ctaccggcag
     1201 gcgcggcgat tcgtgccggc gactagcgct gtacaccgcg gctctggcgc agctccgcgc
     1261 gccgatcctt gagcacctgg atttcggcat gcggccgcca gacgtagtcc ggatcatcgt
     1321 gcgccggggc ccagtagagg tcgcccgaat agcgccaggg gtcgagtacc agcccctgtt
     1381 gcagtgcatc gccgcgggcg ctgatgatca ccgtattgtg ttcgacacga aacgcggatt
     1441 cgtagttggc gacgccccag tgcaggtcga ggctgcgaaa gcgctcctgc tgcaggcgta
     1501 tcatcaggtc gcgagcccag tgcacgcaca ggccacgatc cctgatgccg aggttgacca
     1561 gggtgttgtg aaacagcggt gggccgacga tatcgtattc gcgcgccagc tggtggctgt
     1621 aatcgatcgc cacgcgggcc gcgcgccgcg cctcgtcggg atcgatatcg tcgccgagcg
     1681 acaggatagc aagccccagc tggtcgtcgc gcgcctgctc cgcgtccggg tcgccgatac
     1741 gcgcgccgct gccggcgcac gcctgcagca ccagcagcaa cagcagcgaa accagggtct
     1801 tcgtcgaaat cggggcaatt gctttcatcg agatacgcgc tcggaatgcg atctaaggta
     1861 tcccattcgg ccgtcgcttg tcgagtgccc gcgcgccgcg ccggcaggtg agacaagggt
     1921 atcaactctg ctacactgcg ggctgcattc acagtcgcgg atacgccatg accagcctga
     1981 ccgaacgcct cgaaaacggc gagatcctgt tgctcgacgg cggcgtcagc accgagattc
     2041 gtcgtcgcgg agtagccctc gacgaaaatg tctggagtgg gctcaccacc aagacccacc
     2101 cagaccaggt gcgcgaggtg cacgaggatt acatcgccgc gggtgcacag gtgatcaccg
     2161 ccaataccta ttcgaccgcg cgccacgtgc tggagagcat caatctgggt cacgaggcca
     2221 agctgctgaa tttcaagtcg gtgcagcttg cccgcgaagc ccgcgacgac atggcgcggg
     2281 aggacgtcta tatcgccggt tcgatgtcga gcatgccgcc gctgaccagc caccgcgagg
     2341 tcgccatcga cgggcacgtc gagtccagct accaggaact cgccgaagtg ctggccgagg
     2401 ccggcgtcga cctgatcatc gccgagatga tgcgcgacat taaaaacgcg agcatcgtca
     2461 tcaaggccgc cgtttcgacg gggctgccgg tgtggatcgg ctacagcgcg atgctcgccg
     2521 acaacggcgt cgacgtgcgc agcctgcgct ggaag
//
LOCUS       DATCZV010000106         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_166523, whole genome shotgun sequence.
ACCESSION   DATCZV010000106 DATCZV010000000
VERSION     DATCZV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166523"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3601
                     /locus_tag="VIW27_02415"
     CDS             <1..>3601
                     /locus_tag="VIW27_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEY5738546.1"
                     /translation="RDGLAGIDQGSVIWDGLDGKGRLVADGDYRLRLAKAAGGDIAEV
                     LVHVDTNRSSLLRAINSEFELFNNLTCELDTVYAVNMLESEEQLVIEAGLNDIFALNA
                     SGSEARAIIPASWFGDTYPYDTQASKDGSVIAFSRFDPDQPWWLDGSAVWAADGDGGN
                     LREVSSEIEDGWVLSDDGSRIYHRVFETAVYATDVASGAEQHLYDAYDDIEIEADSFK
                     PQPGGSKLLLIDAAVEDTAPGVVLVDADDPGFEPVLLAFNWRNYRDPNLPRYAWSPDG
                     SKILVTSYIDGKLSVFDSRGQLLDEFDSPAGPENAEFGDPAWSSNAAEVAISIRSFGR
                     EDESLGDSSGIYVLDLDENSIDKVAAIGFGGGGDFFDLSSYHVSTWDGSDWVERGVLH
                     YRRFYREQQLDLSAYLPDADGEYKLRIRQVGKEAAHVESVALLSGGRRAIPSSAIKLE
                     QSLVASLVDRFTDGPRGEDALASVSHNDYEVLDLFESEMQVEWQNLPHSGRVLLALNA
                     REEALSKLNTLPFTYAGDDDGGFPYLLAANSPLEIDGELGPAEILDGPLFKQFSRPGT
                     GHPSATVYGYVGSDSEYLYGALDFTVDNTIDGDLDWASMRVMTAQGWREYRVTMNDDR
                     YGRVNFTRTPAVPFTHKYYEFRIPLAELDRKPGDTVAVSFQGYGTAALVVDDDEALPF
                     EGRLLWPPGDRALLYDAYGEGKWAIDLDDGNRIRELFTDWPGGSQDIFDAFFTDSGRK
                     LLFQSDRASNDPASICFDTGTDLWSYESLLNLVVDLRATRSNSAGGIILSGTVADLNF
                     ERYTLEYASRDAPDSWAPIFTGSTVAVVDARFTTWAPPAVDKYLLRLTAYDRAGNSRS
                     VVRQASWGLSSAITDLSLAPEYVSPNGDGVQDEASLHYRVLEPVHLDFSIYNEQGDVV
                     RTFTRDHAVIGTEHDLVWDGRNNQGLPVADGRYRVAVLGFEFFVNVDSTPPEILSLET
                     RDARQTRIINREEFVVASPGLDWAIREASDYDIEIETGDGAQPDSWRPFAQPIVRDNR
                     VETIAIDEDDMGLTLQQFVDRAFRLRVTDVAGNRTTLAVPPGAQQLILTGKIAEDFDA
                     LDENTYEVIETLAAPISTAIIEYRETGSSAWLEAEIDTADIAENGIGFSWLPAEAAFG
                     KSFVMRVRATDVGARTFYSNEKRLSYQGVEFQYRNLFTSALTPSATLNGLDADG"
BASE COUNT          650 a         1151 c         1180 g          620 t
ORIGIN      
        1 ccgcgacggc ctcgccggta tcgaccaggg cagcgtgatc tgggacgggc tcgacggcaa
       61 gggccggctg gtggccgacg gcgattatcg cctgcggctg gcgaaggccg caggcggcga
      121 tatcgccgag gtactggtgc acgtcgatac caaccgctcg tcgctgctgc gagcgatcaa
      181 cagcgagttc gagcttttca acaacctgac ctgcgaactg gatacggttt acgccgtgaa
      241 catgctcgaa tccgaggagc aactggtaat cgaggccggg ttgaacgata tcttcgcact
      301 caatgccagt ggcagcgaag cacgcgcgat cattccggcg agctggttcg gcgataccta
      361 tccctacgac acgcaggcgt cgaaggacgg ctcggtgatc gccttctcgc gcttcgatcc
      421 cgaccagccg tggtggctcg acggctcggc ggtgtgggcc gccgatggcg acggcggcaa
      481 cctgcgcgag gtgagctcgg aaatcgagga cggctgggtg ctcagcgacg acggcagcag
      541 gatctaccac cgcgtgttcg aaaccgcggt ctacgcgacc gacgtcgcca gcggcgccga
      601 gcagcatctg tacgatgcct acgacgatat cgaaatcgaa gccgattcgt tcaagcccca
      661 gcccggcggc agcaagctgc tgttgatcga cgccgcggtc gaggacaccg cgccgggcgt
      721 ggtgctggtc gatgccgatg acccgggttt cgagccggtg ctgctggctt tcaactggcg
      781 caactaccgg gatccgaacc tgccgcgcta cgcctggtcg cccgacggca gcaagatcct
      841 ggtgaccagt tacatcgacg gcaagctgtc ggtgttcgac agccgcggcc agctgctcga
      901 cgagttcgat tcgccggccg gcccggaaaa cgccgagttc ggcgatcccg cgtggtcgtc
      961 gaacgctgcg gaggtcgcga tcagcattcg cagcttcggc cgcgaggacg aatccttagg
     1021 cgacagcagc ggcatctacg tgctcgatct cgatgaaaac agcatcgaca aggtcgccgc
     1081 cattggcttc ggcggaggcg gtgacttctt tgacctgtcg tcgtaccacg tgtccacctg
     1141 ggatggcagt gactgggtcg agcggggcgt gctccattac cggcgctttt accgcgagca
     1201 gcaactcgac ctgtccgctt acctgcctga cgcggacggc gaatacaagc tgcgtattcg
     1261 ccaggtcggc aaggaagccg cgcacgtcga gagcgtggcg ctgctcagtg gcgggcggcg
     1321 cgcgattccg agcagcgcga tcaagctcga gcaatcgctg gtcgccagcc tggtcgaccg
     1381 cttcaccgac gggccgcgcg gcgaggacgc actggccagc gtcagtcaca acgattacga
     1441 agtgctcgac ctgttcgagt cggaaatgca ggtcgagtgg cagaacctgc cgcactccgg
     1501 gcgcgtgctg ctggcgctca atgcgcgcga ggaagcgctc agcaagctga acaccttgcc
     1561 gttcacttac gccggcgacg acgacggcgg ttttccctac ctgctggccg ccaactcgcc
     1621 gctggaaatc gacggcgaac tgggtcctgc cgagattctc gacgggccgc tgttcaagca
     1681 gttcagtcgg cccggcaccg gccacccgtc ggcgactgtc tacggttatg tcggcagcga
     1741 cagcgagtac ctgtacggcg cgctggattt caccgtcgac aataccatcg atggcgatct
     1801 cgactgggct tcgatgcggg tgatgaccgc gcagggctgg cgggaatatc gcgttacgat
     1861 gaacgatgat cgctatggcc gggtcaactt tacgcgcacc ccggcggtgc cgttcacgca
     1921 taagtattac gagttccgca ttccgctcgc cgagctggac cgcaagcccg gcgataccgt
     1981 ggctgtcagc ttccagggct atggtaccgc ggcgctggta gtcgacgacg acgaggcgct
     2041 gccgttcgaa ggccgcctgc tgtggccgcc cggcgaccgc gcactgctct acgacgccta
     2101 cggcgagggc aagtgggcga tcgatctcga tgacggcaat cgcattcgcg agctgtttac
     2161 cgattggccg ggcggcagcc aggatatttt cgatgcgttt ttcaccgact cgggccgcaa
     2221 gctgctattc cagtctgacc gcgccagcaa cgatccggcg agcatctgtt tcgataccgg
     2281 caccgacctg tggtcctacg aatcgctgtt gaacctggtc gtcgacctgc gcgcgacccg
     2341 ctccaacagc gccggcggca tcattctttc gggcaccgtg gcggacctga atttcgaacg
     2401 ctacacgctc gagtacgcga gccgggacgc gcccgatagc tgggcaccga tcttcaccgg
     2461 ctctaccgtg gcggtggtcg acgctcgctt cacgacctgg gcgccgcccg cggtggacaa
     2521 gtacctgctg cgactgaccg cctacgatcg cgccggaaac tcgcgtagcg tggtgcgcca
     2581 ggccagctgg ggattgagct cggcgatcac cgatctgtcg ctggcaccgg agtacgtctc
     2641 gccgaacggc gacggcgtgc aggacgaggc cagcctgcat taccgagtgc tcgagccggt
     2701 acacctggat ttcagcattt acaacgagca gggcgatgtg gtgcgcacct tcacccgcga
     2761 ccacgcggtg atcggcaccg agcacgacct ggtctgggac gggcgcaaca accaggggct
     2821 gccggtggcc gacggacgct accgcgtcgc tgtgctcggc tttgagttct tcgtcaacgt
     2881 cgattcgacg ccacccgaga tcctctcgct ggaaacccgg gacgcccgtc aaaccaggat
     2941 catcaaccgg gaggaattcg tcgtcgcctc gcccggcctc gactgggcga ttcgcgaggc
     3001 cagcgattac gacatcgaaa tcgaaaccgg cgacggcgcg cagcctgaca gctggcggcc
     3061 gttcgcgcag ccgatcgtac gtgataatcg ggtggaaacc atcgctatcg acgaggacga
     3121 catgggcctc acgctgcagc agttcgtcga ccgcgcgttt cggctgcggg tcacggacgt
     3181 cgccggcaac cgcaccacgc tggcggtgcc gccgggggcg cagcagctga ttttgacggg
     3241 caagatcgca gaggatttcg acgcgctcga cgagaatacc tacgaggtca tcgaaaccct
     3301 cgcggcgccg atctcgacgg cgataatcga gtaccgcgag accggcagca gcgcctggct
     3361 ggaggcggag atcgataccg ccgatatcgc cgagaatggc atcgggttca gctggctgcc
     3421 ggccgaggcg gccttcggca agagcttcgt gatgcgcgtg cgcgccaccg atgtcggcgc
     3481 gcgaactttt tactccaacg agaagcgcct gtcctaccag ggcgtggaat ttcaataccg
     3541 gaacctgttc accagtgcgc tgacgccgag cgcgacgctc aatgggctgg atgccgacgg
     3601 c
//
LOCUS       DATCZV010000107         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_431568, whole genome shotgun sequence.
ACCESSION   DATCZV010000107 DATCZV010000000
VERSION     DATCZV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431568"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VIW27_02420"
     CDS             <1..346
                     /locus_tag="VIW27_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY5738547.1"
                     /translation="KEQPSLICALDRLSDPGSYGFSVECEGERVDGFVVRRDGECYAY
                     RNSCPHTGAPLDWVEHQFLDLDGQLIQCAVHDARFLIDTGECILGPCPGAYLEPLPIR
                     VEDENIYLLGRA"
     gene            complement(325..1644)
                     /gene="rimO"
                     /locus_tag="VIW27_02425"
     CDS             complement(325..1644)
                     /gene="rimO"
                     /locus_tag="VIW27_02425"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY5738548.1"
                     /translation="MSAAVPSIGFVSLGCPKATVDSEQILTQLRAEGYALAPDYESAD
                     LVIVNTCGFIDAAVEESLDSIGEALDRNGRVIVTGCLGARAEVIEERYPSLLAVTGPH
                     ARDQVMRAVHRHLPAPHDPFVDLVPKQGVRLTPQHYAYLKISEGCNHRCSFCIIPSMR
                     GDLVSRPIDEVMEEAQLLVDAGIREILVISQDTSAYGVDCRYATRFWRGQPLRTRFTE
                     LARALGQFGVWVRLHYVYPYPHVDEVIPLMAEGMILPYLDIPFQHASPKILQAMRRPA
                     NRERVLERIAGWREICPELVIRSTFIVGFPGETDEDFETLMEFLDAAQLDRVGCFTYS
                     PVDGAAANALPDPVPAEIAEQRYHRFMIHQQAISRLKLEKRVGQQLEVLVESASDGGY
                     LARSYADAPEIDGLVHVESSQVLQAGEFCRVDITGADEYDLYARPSR"
     gene            1873..2628
                     /locus_tag="VIW27_02430"
     CDS             1873..2628
                     /locus_tag="VIW27_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738549.1"
                     /translation="MKSLKFLLPILLLLALAPGARAQLLDQGLNSIMEIIDKAIAELN
                     GVTPEGGEDAADGEAADPDRLENATTTIVNLSRTLGTLSFSRLQCGEAGVLAEFTSRV
                     QQMPVASRDAMRDAFQEGFDKSKEDSPLLSQDECDRLTASRQRREAATAANVKPVEDK
                     PAPAEQPQEKVAEEASEDPALRHLRIAELSGQLAYRRRVCDGEQVFNRDYNEYLESIP
                     QEYREQAKEAYWKGYQHGKRLNKNFTREHCPRA"
BASE COUNT          500 a          829 c          868 g          464 t
ORIGIN      
        1 gaaggaacag ccgagcctga tttgcgcact ggatcgattg tccgatccgg ggtcttacgg
       61 cttctcggtc gaatgcgagg gcgagcgcgt cgacggtttc gtggtgcgtc gcgacgggga
      121 gtgctacgcc tatcgaaaca gttgcccgca taccggcgcg ccgctcgact gggtcgagca
      181 ccagttcctc gatctcgacg ggcagctgat acagtgcgcg gtgcacgacg cgcgcttcct
      241 gatcgatacc ggtgaatgta tcctcgggcc ctgtcccggc gcgtatctcg agccgctgcc
      301 gattcgcgtc gaggacgaga atatttacct gctcgggcgc gcgtaaaggt cgtactcgtc
      361 ggcgccggtg atatcgaccc ggcagaactc tcccgcctgc agaacctggc tgctctcgac
      421 gtgcaccagg ccgtcgatct ccggcgcatc ggcgtaactg cgcgccaggt agccgccatc
      481 gctggcggat tcgaccagca cctcgagctg ctggccaacg cgtttttcga gctttagcct
      541 gctgatcgcc tgttggtgga tcatgaagcg gtgatagcgt tgctcggcga tctccgcggg
      601 taccgggtcg ggcagtgcgt tggccgcggc gccgtcgacc ggcgaatagg taaagcagcc
      661 gacgcgatcg agttgcgcgg cgtcgagaaa ctccatcagg gtttcgaaat cctcgtcggt
      721 ttcgcccgga aaaccgacga tgaaggtgct gcgaatgacc agctcggggc agatctcgcg
      781 ccagccggcg atgcgttcga gcacgcgctc gcggttggcc ggccgccgca tcgcctgcag
      841 aatcttcggg ctcgcgtgct gaaacgggat atccagatag ggcaggatca tcccttccgc
      901 catcagtggg atcacctcgt cgacgtgcgg gtagggatag acgtagtgca ggcgtaccca
      961 gacgccgaac tggccgagcg cgcgcgccag ctcggtgaaa cgggtcctga gcggttgccc
     1021 gcgccagaaa cgcgtcgcgt accggcaatc cacgccgtag gcgctggtat cctgggagat
     1081 caccaggatc tcgcgaatcc ccgcgtccac cagcaattgt gcctcttcca tgacctcgtc
     1141 gatcggccgg ctgacaaggt cgccacgcat cgacggaatg atgcaaaagc tgcagcgatg
     1201 attgcaaccc tcggaaattt tcaggtaggc gtagtgctgc ggtgtcagcc gcactccctg
     1261 ctttggcacc aggtcgacga acgggtcgtg cggcgccggc aggtgccggt gcaccgcgcg
     1321 catgacctgg tcgcgggcgt gcggtccggt taccgccagc agtgacgggt agcgctcctc
     1381 gatcacttcg gcgcgcgcac cgaggcagcc ggtgacgatc acccggccgt tgcggtctag
     1441 cgcctcgccg atgctgtcga gcgactcctc caccgccgcg tcgatgaaac cgcaggtgtt
     1501 gacgatcacc aggtccgccg actcgtagtc gggcgcgagc gcatagccct cggcgcgcag
     1561 ctgggtaaga atctgctcgg agtcgaccgt ggccttcggg cagccgagac tgacgaaacc
     1621 aatgctgggc acggcagcgc tcacgacggc aggcacggcg cgcggcggtc ggcacgcagc
     1681 gctggcggct gcaatcgcgg gatcgcgggg cggtgatcga aagagcgcag gaaattcaag
     1741 ctggaaccgg gcctggaaac tcgccgcatt atctgtcaat tcgtgaacta gcgcaaaaat
     1801 gcgtctataa ctagctggat gattgcaatt aaccgtgatt taatccgcca gaccagctgc
     1861 tgtcgtttat cgatgaaatc cctgaaattc ttgctgccga ttttactgct gctcgcgctt
     1921 gcgccgggcg cgcgcgcgca gttgctcgac cagggcctga actcgatcat ggaaattatc
     1981 gacaaggcga tcgccgagtt gaacggggtt actcccgaag gcggcgaaga cgctgccgat
     2041 ggcgaggcgg ccgatccgga caggctcgag aatgcgacta ccaccatcgt caacctgtcg
     2101 cgtacgctcg gcaccttgag tttttcgcgg ctgcaatgcg gcgaagccgg cgtgctggcg
     2161 gaatttacca gccgcgtgca gcaaatgccc gtggcaagcc gcgacgcgat gcgtgacgct
     2221 tttcaggaag gcttcgacaa gagcaaggaa gacagtccgc tgctgtcgca ggacgagtgc
     2281 gatcgcctga ccgcgtcgcg ccagcgccgc gaggccgcga cggcggccaa cgtcaagccg
     2341 gtcgaggata agcccgcgcc ggccgagcag ccgcaggaga aagttgccga agaagcgtcc
     2401 gaggatcctg cgctgcgtca cctgcggatc gccgaactct ccggtcagct cgcctaccgg
     2461 cggcgggtct gcgacggcga acaggtgttc aaccgcgact acaacgaata cctcgaatcg
     2521 attccgcagg agtaccgcga acaagccaag gaggcttact ggaagggata ccagcacggc
     2581 aagcggctga acaagaactt cacccgcgag cattgcccgc gcgcctgaca cgcggccggc
     2641 ttgtcagtcg tccaggtaac t
//
LOCUS       DATCZV010000108         4233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_272440, whole genome shotgun sequence.
ACCESSION   DATCZV010000108 DATCZV010000000
VERSION     DATCZV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272440"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VIW27_02435"
     CDS             complement(<1..470)
                     /locus_tag="VIW27_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5738550.1"
                     /translation="MKTPDAIGSLLQAVAVMSAGKLLFAVQDAIIKEMSGAYPVHQIM
                     TIRGLVAIPLLLLLIHFSGGLGSLRHHRPLLHLVRGALMLTAFMCFYVALAGTALTTA
                     TALFFTAPFFITLMSIPLLGERVGLRRFAGIGVGFGGVLLVLRPEAGSFGWYDLL"
     gene            complement(467..1951)
                     /gene="ppx"
                     /locus_tag="VIW27_02440"
     CDS             complement(467..1951)
                     /gene="ppx"
                     /locus_tag="VIW27_02440"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY5738551.1"
                     /translation="MEQFAAIDLGSNSFHMVVAREEQHALTVIDRERDMVRLAAGLDE
                     RNLLTPAACERAIGCLARFGERLRGLKPDNVRVVGTNALRKAKNSRQFIARAEQALGF
                     SIEIISGVEEARLVYIGATQSLQGDRGRRLVIDIGGGSTELIVADRNEPVFLESLHMG
                     CVSSSEKFFPDGRIDRKRMKRAILNAHLELEPHVATLKGLAAEDVIGTSGTARAIDTV
                     LRENRLSETGITRKGLKKLVSKVVKAGHSEQLDLAGLSVERRPVFAGGVAVMMAIFKA
                     LDLDFMRVSDGSLREGVLHDLIGRVLYEDRRAITVLEMMKRHHVDMEHGNRVRNTALA
                     LFDQVGQAGLLERERRLLGWAAQLHEIGLMIAHNQHHRHGGYLLQHMDLPGFSRQEQG
                     ALSIMVRLHRRKYHTALIEDSAYVRSDALGLMTRLLRLGVVFNRGRTPIELPRIEVLL
                     DSAQSMTLKIDAAWLANHPLTAADLEQEAELLESAGYALRILRR"
     gene            complement(1953..3059)
                     /locus_tag="VIW27_02445"
     CDS             complement(1953..3059)
                     /locus_tag="VIW27_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HEY5738552.1"
                     /translation="MNVPETMRAVVLTGHGGLDKLEYREDWPTPVPGPGEVLVRVGAC
                     GLNNTDINTRTAWYSKGVTEGITAAGGSHGFDTADAGGGSWGSASIDFPRIQGADVAG
                     HIAAVGDGVDPRRVGERVILDPWLLAAGDWLDASRSGYLGSECDGGYADYTRIRSENA
                     IRIECDLSDAELATFPCAYTTAENLTARSGLRPGETVVVNGASGGVGSAAIQLCRLRG
                     ARVLAIASPAKAAALEGLGAEQVIDRHCADLGAAIRDAAGGAVDVALDVVGGNSFMPL
                     IDALRQGGRYSTSGAIAGPMVDFDLRQLVYKDLQLTGATIVPPGTMHRIVNLIAAGQL
                     RPLLAQTFRLAELGRAQQAFLQKRHVGNIVVASR"
     gene            complement(3256..3666)
                     /locus_tag="VIW27_02450"
     CDS             complement(3256..3666)
                     /locus_tag="VIW27_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738553.1"
                     /translation="MEYIQVREYGELPEIGQRAPFKAVLAVENAVSRERQREVADWLV
                     AMGGMYIMVCGQDCESWQHAIRHANLERVPLDDMQPQQFVMITMHMYEKLRGVFRYAS
                     KHARHTHLKLDNLLTVHIANQNRELEYQNLFGKR"
BASE COUNT          718 a         1384 c         1395 g          736 t
ORIGIN      
        1 agcaggtcgt accagccgaa gctgcctgct tccggtctca gcaccagcag cacgccgccg
       61 aagccgacac cgataccggc gaaacggcgc agcccgacgc gctcgccgag cagcggaatc
      121 gacatcagcg tgatgaaaaa cggcgcggtg aaaaacagcg ctgtggcggt ggtcagcgct
      181 gtaccggcca gcgcgacgta aaagcacatg aacgcggtca gcatcagcgc gccgcgcacc
      241 aggtgcagca gcggccggtg gtggcgcagc gagcccagcc cgccgctgaa atgaatcagc
      301 agcagcagca gcggaatcgc gaccaggccg cgaatggtca tgatctggtg taccgggtaa
      361 gcgccgctca tttccttgat gatcgcgtcc tgcaccgcga acaaaagttt accggccgac
      421 atcacggcga ctgcctgcag cagcgaaccg atggcgtccg gcgtcttcaa cgccgcagga
      481 ttcgcagcgc gtagcccgcc gattccagca actcggcttc ctgctccagg tcggcggcgg
      541 tcagcgggtg attcgccagc caggccgcgt cgattttcag cgtcatcgac tgcgccgaat
      601 ccagcagtac ctcgatcctg ggcagctcga tcggcgtgcg gccgcgattg aagaccacgc
      661 cgagacgcag caggcgggtc atcaggccga gcgcgtcgga gcgcacgtag gcgctgtcct
      721 cgatcagcgc ggtatggtac ttgcgccgat gcagccgcac catgatcgac agcgcgccct
      781 gttcctggcg cgagaaaccc ggcagatcca tgtgctgcag caggtagccg ccatggcggt
      841 ggtgctggtt gtgggcgatc atcaggccga tttcgtgcaa ctgcgcggcc caaccgagca
      901 gccggcgctc gcgttccagc aggcccgcct ggccgacctg gtcgaacagc gccagcgccg
      961 tgttgcgcac gcggttgccg tgttccatgt cgacgtgatg ccgtttcatc atctccagca
     1021 ccgtgatcgc acgccggtct tcgtagagca ctcgcccgat caggtcgtgc agcacgccct
     1081 cgcgcagcga gccgtcggag acacgcatga aatccaggtc cagtgccttg aagatcgcca
     1141 tcatcaccgc gacgccgcca gcaaataccg ggcgccgctc gacgctgaga cccgccagat
     1201 cgagctgctc gctatgcccg gccttgacca ccttgctgac cagtttcttg aggcccttgc
     1261 gcgtgatgcc ggtctcgctg agccggtttt cgcgcagcac ggtatcgatc gcccgtgcgg
     1321 tacccgaggt gccgatcacg tcctcggccg cgagaccttt cagcgtggcg acgtgtggtt
     1381 cgagctcgag gtgagcgttg aggatcgcgc gtttcatgcg cttgcgatcg atccggccgt
     1441 cgggaaagaa tttctcgctg ctgctgacgc aacccatgtg caggctttcg aggaataccg
     1501 gttcgttcct gtcggcgacg atcagctcgg tgctgccgcc gccgatatcg atcaccaggc
     1561 gcctgccgcg atcgccctgc aggctctggg tggcgccgat ataaaccagc cgcgcctcct
     1621 ccacaccgga gatgatttcg atcgaaaagc cgagcgcctg ctcggcgcgg gcgatgaact
     1681 gacgcgagtt tttggccttg cgcagcgcgt tggtgccgac cacgcgcacg ttgtcgggtt
     1741 tcaggccgcg caggcgctcg ccgaaacgcg ccaggcagcc gatggcccgc tcgcaggctg
     1801 caggcgtcag caggttgcgc tcgtcgagcc cggcagccag ccgcaccatg tcgcgttcgc
     1861 ggtcgatcac ggtaagcgcg tgctgctctt cgcgcgccac taccatgtga aagctgttcg
     1921 aacccaggtc gatggcggca aactgttcca agttagcggc ttgccacgac gatgttgccg
     1981 acgtgccgtt tctgcagaaa ggcttgctgc gcgcggccga gttccgccag gcggaaggtc
     2041 tgtgccagca acggccgcaa ctggccggcg gcgatcaggt tgacgatgcg gtgcatggtg
     2101 cccggcggca cgatggtggc gccggtcagc tgcaggtcct tgtagaccag ctgacgcagg
     2161 tcgaagtcga ccatcgggcc ggcgatcgcg cccgaggtgg agtagcggcc accctggcgc
     2221 aacgcgtcga tcagcggcat gaaggaattg ccgccgacca cgtcgagcgc cacatcgacc
     2281 gctccgccgg cggcgtcgcg gatcgcggct ccgagatccg cgcaatggcg gtcgatcacc
     2341 tgctcggcgc cgagaccctc gagcgcggcg gcctttgccg gcgacgcgat cgcgagcacc
     2401 cgcgcaccgc gcaggcggca cagctggatg gcggccgaac cgacgccgcc cgaggcgccg
     2461 ttgacgacca cggtttcgcc cggccgcagg ccgctgcgtg cggtcaggtt ctcggccgtg
     2521 gtataggcgc acggaaaggt cgccagctcg gcgtcgctga ggtcgcattc gattcgaatc
     2581 gcgttctcgc tgcgaatccg cgtgtagtcg gcgtaaccgc cgtcgcactc ggaaccgaga
     2641 tagcccgagc gcgatgcgtc gagccagtcg ccggcggcca gcagccacgg gtcgagtatt
     2701 acgcgctcgc cgacgcgtcg cggatcgaca ccatcgccga ccgcggcgat gtgcccggcg
     2761 acgtcggcgc cctgaatgcg cggaaagtcg atgctggcgc tgccccagct gccgccgccg
     2821 gcatcggcgg tatcgaagcc gtggctgccg ccggcagcgg tgatgccctc ggtgacgccc
     2881 ttcgaatacc aggcagtgcg ggtgttgata tcggtattgt tgagcccgca ggcgccaacc
     2941 ctgaccagta cctcgccggg tcccggcacc ggggtcggcc agtcctcgcg gtactcgagc
     3001 ttgtcgagcc cgccgtgacc ggtcagcacc acggcacgca tcgtctcggg aacgttcatt
     3061 accagctccg gagtgtattc ggcagcgatg gtatgtaaag gcgtcggggg agtcacggtt
     3121 ttttgtcgct gcgccagcgg cgatgcgtcc gaaactgccg ccgaaggcaa gcggcgaatc
     3181 ttcgccgagc cgacagctcg cggtcgggtt gtcgagcagg cggccggcga aatcgtgagg
     3241 atcgattgca tcgcgtcagc gcttgccgaa cagattctga tactcgagtt cgcggttttg
     3301 attcgcgatg tggaccgtga gcaggttgtc gagcttgagg tgcgtatggc gagcgtgttt
     3361 gctggcgtag cggaatacac cgcgcagctt ttcgtacatg tgcatggtta tcatcacgaa
     3421 ttgctgtggt tgcatatcgt cgagcggcac tcgctcgaga ttggcatgac gaatggcgtg
     3481 ctgccagctt tcacaatcct ggccgcatac catgatatac atgccgccca tcgccaccag
     3541 ccagtcggca acctcgcgtt gccgctcgcg gctgaccgcg ttctcgaccg cgagcacggc
     3601 cttgaagggc gcgcgctggc cgatctccgg tagttcgccg tattcgcgga cctgtatgta
     3661 ttccatgcct gtatcctgct gtgagcacgg tcccggataa aggtttaccg ggatgccctc
     3721 ggcgccgctt cgtgcggctt ttgggagtca ttctcacata ctcgccggga cagtggcaaa
     3781 aagccgcatg cctcacgccg cgccaggaag cggcgagtgc aagtcgccga agctgtccga
     3841 ccaggggatt tgcggctgtc gaagccgttt tcgggcagtg ccccggatct tgcctggagc
     3901 agtgcaggcg atggccgcca gccgagcgca cagcgctccg atgaaggcac acagcggcgc
     3961 gccaatcgtc gcgccgcagg tcagacccgg gcacgactcg cgggcggtga gcgactcgcg
     4021 tcgccaggcg agtcatcgat tcgcgccggc gggtaaaacg cgcgcaagcc gcacttgccc
     4081 gaaactcgcg tagatgctta actcgggtta tgagctggca actatcgatc aggatgttcc
     4141 gtgacctgtt ccgcggcgac gaggagttcg cggtcatcga tccgcgtctg cagtcggcat
     4201 tcgtcgagcg ccacctgctg gcggcgtcca acc
//
LOCUS       DATCZV010000109         3982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_61147, whole genome shotgun sequence.
ACCESSION   DATCZV010000109 DATCZV010000000
VERSION     DATCZV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61147"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3130
                     /locus_tag="VIW27_02455"
     CDS             <1..3130
                     /locus_tag="VIW27_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5738554.1"
                     /translation="LGPLDDEARNALQALLDSGELSADGQSSNAAQAANGQLEFAEPV
                     APSLGTGSSESASFEDAAAAFAEDSAPEFDTSDMLGMLASELHDITADLAYLSQTVAT
                     AADEAELRPAADSYEELVSRVGTVARELGLEGLLLICEFITRNLQLVAALPVDQRAAS
                     RDLFAGWTRVVIDHLTQPQDDALCLAVVAYLEDSNWPEALQYRDVRGLIEGLTHELEV
                     TGDSEVEAREILATPDDVSLSMSDDASPELIDAFFAESPGHAETFSRLMEAISRGEEI
                     QQNVEAAQRIAHTLKGSGNLVGVKGIANLAHHIEDIFEYIARHEITPPAALAGTMQEA
                     ADTLEAMLESLQGISSPPEDAQRVLQDVLDWANRIDSGNMRQQDYEAAPARAAAEETQ
                     AEQGEELAEDFVDRRKSPETEKVLAASREEAVRVPLRVLDEIFRIVSETAITIGQIQE
                     RLNRLNINEKMIRKNDASLQQMRFELESLVSVRGMAARHRSELAQSNANFDPLELDEY
                     DEFYGATHSYIEGVADSREILRAFSTEVYDLNALFLAQQRLNKELQQMVMSTRMVPVS
                     IICPRLQRAVRQACRATGKQVELTIIGQDLLLDGDVLNKLADPLMHMLRNAVDHSIEP
                     GELRIERGKPKSGEITLTFHQQGNNIVVDCCDDGKGLDYERIREVAIGKQMLSANEHP
                     DNPTLAQMILRPGFSTSATVTHVSGRGVGMDVVHNTIQSLNGTMDIGDAKQGGTRISL
                     RLPITLLTSHCLLVGVGDDQVYAIPSISLNQIMSPGSGTLTRSGETLSYQFADDSYAA
                     CSLNTLVGAADDFISEELDACSVLLVQTADSVSAVVVERVITSYDLVVKNMGAYIKSI
                     DGIAGVAMLGNGTVVSVLDLASLLEADKRPAALADGSRTAANRSESPEMALPMVMIVD
                     DSLSVRNSLSQLMKDGGYRTVTARDGLEAVKLLETEVPDIVLTDLEMPRMNGLDLTSF
                     IRKSPQWAPLPVVMITSRTMAKHRQQAEIVGVNRYLTKPFSEDEVLASIDEQLSASFR
                     FAEQAEKTVRPE"
     gene            complement(3410..3775)
                     /locus_tag="VIW27_02460"
     CDS             complement(3410..3775)
                     /locus_tag="VIW27_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5738555.1"
                     /translation="MPIRKILLVDDSPAQLEAMQQAVASVDAHVFTAISGKEAVEKAR
                     QEKPDIIFMDIVMDDLDGYGACREIKQGAETSNIPVVFVTTKNSRADKLWADKQGAMA
                     MINKPFEPEELLEQIRRIG"
BASE COUNT          812 a         1233 c         1209 g          728 t
ORIGIN      
        1 gctcgggcca ctcgacgacg aggcgcgcaa tgcgctgcag gcattgctcg acagcggcga
       61 gctcagcgcc gacggccagt cttcgaacgc ggcgcaagcc gccaacgggc aactcgaatt
      121 cgccgaacct gtcgcgccgt cgctcgggac cgggagcagc gaatcggcgt cgttcgaaga
      181 cgccgcagcg gcatttgccg aggactccgc acccgagttc gataccagcg atatgctcgg
      241 catgctcgcc tcggaattgc acgatatcac tgccgatctg gcctacctgt cgcaaaccgt
      301 tgccacggcc gccgacgagg cggaattaag gccggcagcc gacagctacg aggaactggt
      361 ctcgcgcgtc ggcacggtgg cacgcgaact cggtctcgag ggtctgctgc tgatctgcga
      421 attcataacc cgcaacctgc agttggtcgc cgcgctgccg gtcgaccaac gcgccgcgtc
      481 ccgcgacctg ttcgccggct ggacgcgagt ggtcatcgac catctgacgc agccccagga
      541 tgatgcgctg tgcctggccg tggttgccta cctcgaggat tcgaactggc cagaggccct
      601 gcaataccgc gacgtacgcg gcttgatcga gggactgacg cacgaactcg aggtcaccgg
      661 cgatagcgag gtcgaggccc gcgaaatcct ggccacgccc gacgatgtct cgctgagcat
      721 gtcggacgac gccagccccg aactgatcga tgctttcttc gccgaatcac cgggccatgc
      781 ggaaaccttt tcccggctga tggaagcgat cagccgcggc gaggaaattc aacagaatgt
      841 cgaggctgcg cagcgcatcg cgcacaccct caaaggatcg ggcaacctgg tcggtgtcaa
      901 gggtatcgcc aacctggccc accatatcga ggatattttc gaatatatcg cccgtcacga
      961 gatcactccg ccggccgcgc tggccggcac gatgcaggaa gccgccgata ccctcgaagc
     1021 gatgctcgaa tctttgcagg gtatttcctc gcctcccgag gacgcccagc gcgtgctgca
     1081 ggatgtgctc gactgggcca accgtatcga cagcggcaac atgcgccagc aggactacga
     1141 agccgcgccg gcgagagcag ccgcggagga gacgcaggcg gagcagggcg aggagctggc
     1201 ggaggatttc gtcgatcgtc gcaagagccc ggaaaccgag aaagtactgg cggcatcgcg
     1261 cgaagaagcg gtgcgcgtac cgctcagggt actcgacgag atttttcgca tcgtcagtga
     1321 aaccgcgatc accatcggcc agatccagga acgcctgaat cgcctcaata tcaacgagaa
     1381 aatgattcgc aagaacgacg cctcgctgca acaaatgcgc ttcgagctcg agagcctggt
     1441 cagcgtgcgc ggcatggcgg cacgccatcg cagcgaactc gcgcagagca acgcgaattt
     1501 cgatccgctg gagctggacg aatacgacga attctatggc gccacgcatt cctatatcga
     1561 gggcgtcgcc gactcgcgcg aaatcctgcg cgctttctcc accgaggtct acgacctcaa
     1621 cgcgctgttt ctcgcccagc aacggctcaa caaggaactg caacaaatgg tcatgtcgac
     1681 gcgaatggtg ccggtgagca tcatttgccc gcgcctgcag cgtgccgtca ggcaggcgtg
     1741 tcgggcgacc ggcaagcagg tcgagctgac gatcatcggc caggacctgc tgctcgacgg
     1801 cgacgtgctc aacaagcttg ccgacccgct gatgcacatg ctgcgcaacg cggtcgatca
     1861 cagcatcgag cccggcgagc tgcgaatcga gcgcggtaaa ccgaaaagcg gggagatcac
     1921 gctgaccttt caccagcagg gcaacaacat cgtcgtcgat tgttgtgacg acggaaaagg
     1981 gctcgactac gagcgcatac gcgaggtcgc gatcggcaag cagatgctgt cggcgaatga
     2041 gcacccggac aacccgacgc tcgcgcaaat gatcctgcga cccggatttt cgaccagcgc
     2101 aacggttacc catgtttccg ggcgcggcgt tggcatggac gtcgttcaca ataccatcca
     2161 gtcgctcaac ggcacgatgg acatcggcga cgcgaagcag ggcggcaccc gtataagcct
     2221 gcgcctgccg atcacgctgc tgaccagcca ctgcctgctg gtgggggtcg gcgacgacca
     2281 ggtttacgca atcccgagta tctcgctgaa ccagatcatg tcgccgggca gcggcacgct
     2341 gacgcgctcg ggggaaactc tcagctatca gttcgcagat gacagttacg cggcctgcag
     2401 cctcaacacg ctggttggcg cggcggatga tttcatcagc gaggaactcg atgcttgcag
     2461 cgtattgctg gtacagaccg ccgacagcgt cagcgcggtg gtcgtcgagc gggtgattac
     2521 cagctacgat ctggtggtca agaacatggg cgcttatatc aagtcgatcg atggtatcgc
     2581 cggtgtcgcg atgctcggta acggtaccgt ggtatcggtt ctcgatctgg cgtccttgct
     2641 ggaagcggac aagcgtccgg cggcgctcgc cgacggcagc cgtaccgccg caaaccgcag
     2701 cgagagcccc gagatggcac tgccgatggt gatgatcgtc gacgactccc tgagcgtgcg
     2761 caactcgctg tcgcaattga tgaaggacgg cggctatcgc acggtgaccg cgcgcgacgg
     2821 gctcgaagcc gtcaaattgc tggaaaccga ggtgcccgac atcgtattga ccgacctcga
     2881 gatgccgcgc atgaacggtc tcgacctgac cagttttatt cgtaaatcac cacagtgggc
     2941 gccgctgccg gtggtcatga tcacctcgcg aaccatggcc aagcaccgcc agcaagctga
     3001 aattgtcggt gtcaatcgtt accttaccaa gcccttcagc gaggacgagg tactggcctc
     3061 gatcgacgag caattgtcgg cgtcattccg ctttgccgag caggctgaaa aaaccgtcag
     3121 acccgaatag agtcccctgt tgatcggcgc tgccgccgag ctgttaacca aaacttcgct
     3181 ttcgattatc gttcgctgcg cgtcacaggg caggcgtaat cggccgatgg cgcttgccgc
     3241 gggagcaggg cagtcatcga cggtgttcgc cgccagtgcc tgaacggcac tcgtgactct
     3301 tccgggctgg tatttcaagc ttatgatttt caaaagaaaa acgcggcttc accgaagccg
     3361 cgtcgctact gcctgcaagc tgcgccgggt gcgatgcccg gcgcggagtt cagccgatgc
     3421 gccgaatctg ctcgagtaat tcttccggct caaagggctt gttgatcatc gccatggcac
     3481 cctgcttgtc ggcccacagc ttatccgcgc ggctgttctt ggtggtgacg aatactaccg
     3541 gaatattgct ggtctccgcg ccctgcttga tttcgcgaca ggcaccgtag ccgtcgaggt
     3601 cgtccatcac gatatccatg aaaatgatat cgggcttttc ctgcctggcc ttttcgaccg
     3661 cttccttacc gctgatcgcg gtgaacacgt gtgcgtcgac gctggcaacc gcctgctgca
     3721 tggcctccag ctgcgccgga gaatcgtcta cgagtaatat ctttctgatc ggcatggcat
     3781 tgtctctcta gtcaatattt gaattgctgt attgaatcta gacgctgcgc tgactatcac
     3841 catgggaata gttcccgaaa acggctgccg atggtgggaa aacttcccga tcaggggttt
     3901 tcggacaggg cctcgggcca atgccgagca gggcgcagcc gcgcatcgaa tccgcgactc
     3961 acgctcggct tcaggcgctg ac
//
LOCUS       DATCZV010000110         6148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_537871, whole genome shotgun sequence.
ACCESSION   DATCZV010000110 DATCZV010000000
VERSION     DATCZV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537871"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /gene="zwf"
                     /locus_tag="VIW27_02465"
     CDS             complement(<1..1227)
                     /gene="zwf"
                     /locus_tag="VIW27_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5738556.1"
                     /translation="MTAVDAFDLVIFGGTGDLAARKLIPAMYHRHCAGQLPANGRIIA
                     LGRNDLGREGYLAAISEKSRSLITDRYLDEAQWSAFAERIDYRQLDADSNEDFERLAG
                     DLRDAAERIRIFYLSTSPDLFAGICQKLESSGLALPNSRVVLEKPLGRDLKSADEINA
                     CVETVFSEQQIYRIDHYLGKEAVQNLMALRFGNSIFEPLWRRGRIRHVQITLAEKLGV
                     EGRGEFYDATGTLRDMVQNHLMQLLCIVAMEPPITMDADNVRDEKLKVIRSLQPFTPE
                     GVLSDTVRGQYLGGEVDGERVAAYAAEPGIAAGSQTETFVALKARIDNWRWSGVPFYL
                     RTGKRMHAQVGEVVIEFDSLPHEIYPASAGASVGNRLYIRLQPQESIELHLMAKQAGD
                     HNVLKPVKLDLDLVGVD"
     gene            1497..1688
                     /locus_tag="VIW27_02470"
     CDS             1497..1688
                     /locus_tag="VIW27_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738557.1"
                     /translation="MPEIDPAGAAATVRFTACNALFMASLPGSRRGRHQPGIAIIFAR
                     YDLPGAHQSQRHPGMTIAE"
     gene            1703..2410
                     /gene="pgl"
                     /locus_tag="VIW27_02475"
     CDS             1703..2410
                     /gene="pgl"
                     /locus_tag="VIW27_02475"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY5738558.1"
                     /translation="MPVTEHKFTAREQMLDALYAALTAELERALAAQASATLLLSGGS
                     TPAPLYRRLASASLDWSRIDVALVDERWVDADDAASNERLLRETLLQGPAAAARFTGM
                     KTSQPTAAAAVDDCNRRYAALPSPYCLCLLGMGSDGHTASLFPGAEGLQPALGAKRHC
                     AALRALPSAVTGDNLERMTMTPWGILQARKLVLLISGEDKWRIYREAGRAGASQALPV
                     SLFLDQQRVPVEVYWAP"
     gene            complement(2462..2881)
                     /gene="arfB"
                     /locus_tag="VIW27_02480"
     CDS             complement(2462..2881)
                     /gene="arfB"
                     /locus_tag="VIW27_02480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018949918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY5738559.1"
                     /translation="MIQIAPGIELPEEEIELSAIRAQGAGGQNVNKVSSAIHLRFDIQ
                     ASSLPEDCKQRLLALRDRRLSKDGVIVIKAQRFRNQEKNRVEALERLRDLIAAALRVV
                     KTRKPTRPTRASRQRRLDDKVQRGRTKRLRGRPPDLE"
     gene            3204..3668
                     /locus_tag="VIW27_02485"
     CDS             3204..3668
                     /locus_tag="VIW27_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5738560.1"
                     /translation="MNSQARRIAIVGSGPGGLSAAAHAAELGVSHVLIEAEAHISNTI
                     FRYQKGKHVMDEPRVLPLRSPLQFTAGKREAVLEAWQNGLQAHGAELLLNSEVSGICG
                     HKGNCEIQLRQGAPVRAEHVVLAIGVQGNPRTLGVPGEDAPIVQYTLDDPAE"
     gene            3705..3827
                     /locus_tag="VIW27_02490"
     CDS             3705..3827
                     /locus_tag="VIW27_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738561.1"
                     /translation="MRALVRSAIARIDRHEFSPQQTAALTSSLISTGIAGQYRD"
     gene            3887..4033
                     /locus_tag="VIW27_02495"
     CDS             3887..4033
                     /locus_tag="VIW27_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738562.1"
                     /translation="MQPFDPRTTELVGGLMDTLFDSVADEESCQPAEFSAVRAKLKSG
                     LSIR"
     gene            complement(4043..4405)
                     /locus_tag="VIW27_02500"
     CDS             complement(4043..4405)
                     /locus_tag="VIW27_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5738563.1"
                     /translation="MVIYAEQRSSTTQFELGELFDKGQASRRDYCEAFRWYRQAAQNG
                     SRRAQHRLASMYARGQGVEQSLPRAYAWCKLAVIQGSRYARLKLGFIEARMSADQLRQ
                     GHCLAENLCDRYVVGRDA"
     gene            complement(4545..4883)
                     /locus_tag="VIW27_02505"
     CDS             complement(4545..4883)
                     /locus_tag="VIW27_02505"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HEY5738564.1"
                     /translation="MRLAATLITALLFGASPAKAGFFTGEQLLVKCNSEEVVHQLACV
                     FYLAGAVDASQTHHRWGDIAQQFCMGESINIVNLRQVYLRYAVDHVEELQLPATGSAL
                     NSFIDAFPCQ"
     gene            5100..5612
                     /locus_tag="VIW27_02510"
     CDS             5100..5612
                     /locus_tag="VIW27_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738565.1"
                     /translation="MLIAALSLLSGPAGAASGVLLLAQADSGGERQGKPRLIDFWQPG
                     DNGQRMNIRGRVTSLDGTPLAGIPISIRQANGDGEYTDRYRGTLLSDEQGRFQFGSVL
                     PGNYTGARHVHIAVYTDGYEYFDTSILFKDDPNQESHYGYGTAIFLEEATVGGETMLF
                     GRFDIMLTPM"
     gene            5658..>6148
                     /locus_tag="VIW27_02515"
     CDS             5658..>6148
                     /locus_tag="VIW27_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY5738566.1"
                     /translation="MKKITTLLILLATLCRPAIAQTPIIADHLVSNDSLPGNAHEFYF
                     TRGIYSGEYDDYDQGGRWAVDYPKADHQFLVALKRLSIVDAYESDNAITLTDPRLRRF
                     PLLYAVEVGSMSLNEEEVEALRDYLLAGGFLVVDDFWGSWAWEQFSTQMHRVLPDRAI
                     VEIP"
BASE COUNT         1135 a         1957 c         1918 g         1138 t
ORIGIN      
        1 atccacgccg accaggtcga ggtcgagctt gaccggcttg agcacattgt gatcgccagc
       61 ctgcttggcc atcaggtgaa gttcgatgct ctcctgaggc tgcaggcgaa tatagaggcg
      121 attgccgaca ctggcgccgg cggacgccgg gtagatctca tgcggcagcg aatcgaattc
      181 gattaccact tcgccgacct gtgcgtgcat gcgcttgccg gtgcgcaggt agaatggcac
      241 ccccgaccag cgccagttgt cgatcctggc tttcagcgcg acgaaggttt cggtctggct
      301 gcccgcggcg atgccgggtt cggcggcgta ggcggcgacc cgctcaccgt cgacctcgcc
      361 gccgaggtat tgcccgcgca ccgtgtcgga aagcacgcct tccggcgtga acggctgcag
      421 cgaacgaatc accttgagct tctcgtcgcg cacattgtcg gcatccatcg taatcggcgg
      481 ctccatggca acgatgcaca gtaactgcat caggtggttc tgtaccatgt cgcgcagcgt
      541 gccagtggcg tcgtaaaatt caccgcggcc ctcgacgccg agcttctcgg ccagcgtgat
      601 ctgcacgtgc cgaatcctgc cgcgccgcca cagcggctcg aaaatcgaat tgccgaaacg
      661 cagcgccatc aggttttgca ccgcttcctt gccgagataa tggtcgatgc ggtaaatctg
      721 ctgctcgctg aatacggttt ccacgcaggc attgatttcg tcggcgctct tgaggtcgcg
      781 cccgagcggc ttctccagca ccacgcgcga gttcggcagc gccagcccgc tgctctcgag
      841 cttttggcag atgccggcga acaggtcggg cgaagtcgac aggtaaaaga ttcgaattcg
      901 ctccgcggca tcccgcagat caccggcgag ccgctcgaaa tcctcgttag aatcggcatc
      961 gagctggcgg tagtcgatgc gttcggcgaa cgcgctccac tgcgcctcgt cgagatagcg
     1021 gtcggtgatc agcgaacggc ttttttcgga aatcgccgcc agataaccct cgcgcccgag
     1081 gtcattacgg ccgagcgcga taatccgccc gttggccggc aattgtcccg cgcagtggcg
     1141 gtgatacatg gcgggaatca gtttgcgcgc cgcgaggtcg ccggttccgc cgaaaatcac
     1201 cagatcgaat gcgtcaacgg cagtcaaggt cttgcccccg gggtaattcg atgattgtgc
     1261 gcatgagtag cgataattat ctagtagatt tacaaattat taaatagcct tgccggctaa
     1321 ataccggtct tcagctatta aggattttca agttattgtt attaaatcga aatatataaa
     1381 aacccggttt tgttcgccgg agccgacagc tggcgggtca gtcaagccat ttgcggttat
     1441 tttgtagttt tgctacattc ggccaccgcc caaactcgat cttcgaggaa ttactgttgc
     1501 ctgaaatcga ccccgccggc gccgcggcaa ccgtcaggtt cacagcctgc aacgcgcttt
     1561 tcatggcgag cttaccgggc tcgcggcgag gccgccatca gccgggcatc gcgatcatct
     1621 ttgcccgcta cgacctgccg ggcgcgcacc agtcgcagcg tcatcccggg atgacgatcg
     1681 ccgagtgagc gcgccgcgaa cgatgcctgt caccgaacat aaattcaccg cccgcgagca
     1741 aatgctcgat gcactgtacg cggcgctgac cgcggaactc gagcgggcac tggccgcaca
     1801 ggcatcggcg actttgctgc tgtcgggcgg ttcgacgccg gcacccctct accggcgact
     1861 ggcgagcgcg tcgctcgact ggtcgcgtat cgacgtggcg ctggtcgacg aacgctgggt
     1921 ggacgccgac gacgcagcca gcaatgagcg cctgttgcgt gaaaccctgc tgcaggggcc
     1981 ggccgcggcg gcgcgcttca ccggcatgaa gaccagtcag ccgacggccg cggcggccgt
     2041 cgacgactgc aaccgccgtt atgcggcgct gccgtccccc tactgcctgt gcctgctggg
     2101 catgggtagt gacggtcaca ccgcatcgct gttccccgga gccgagggcc tgcaaccggc
     2161 gctcggcgcg aagcgtcact gcgcggcgct ccgcgcgtta ccgtcggcgg tcaccggcga
     2221 caatctggag cgcatgacga tgacgccgtg gggcatcctg caggcacgca agctggtatt
     2281 gttgatcagc ggcgaagaca agtggcggat ctaccgcgag gcaggccggg ccggcgcctc
     2341 gcaagccctg ccggtgagcc tgtttctcga ccagcagcgc gtacccgtcg aggtctactg
     2401 ggccccctga ggcgcaaccg ctgtcaaagc ctgcttgccg gcaactttgc ggtgcgcgaa
     2461 ttcactccag gtccgggggt cggcctcgca gccgcttggt gcggccgcgc tgcaccttgt
     2521 cgtcgagtcg ccgctgccgc gaggcgcgcg tcggccgcgt gggcttgcgg gttttaacca
     2581 cccgcagcgc agcagcgatc aaatcgcgca ggcgctcgag cgcctcgacg cgattctttt
     2641 cctggttgcg gaatcgctgc gccttgatga cgatgacacc gtccttgctg aggcgccggt
     2701 cgcgcagcgc cagtagccgc tgcttgcagt cctcgggcag cgacgacgcc tggatatcga
     2761 agcgcaggtg gatcgccgac gaaaccttgt tgacgttctg cccgccggcg ccctgggcgc
     2821 ggatcgccga caactcgatt tcttcctcgg gcaattcgat gccgggagca atctgaatca
     2881 tcgcaaggtc cgtctggaaa aggcgcgatt ataaccgaag cgctgagcgg ccgcgcgtcc
     2941 cgcgggcgcg caggacaacc gccgaacgcc tccgcgggca ggcgcgagac cggcgccgcg
     3001 acgcgcctca ttcaccggtt gactggggta cccggctggt ttctagtggg tacacaaaat
     3061 aataaataac tcataccggg aaaaaccgtt cgaaaccctg cgcacttcac cgtcacctgt
     3121 tgacggtgac tgcgcggcgg tttcggtttc gaagctagac ggctatatgc cgacggcccc
     3181 ggttgctgga tgacgcgaaa ccaatgaatt cccaggcccg ccgcatcgcc attgtcggct
     3241 ccggtcccgg cggtctgagc gcggccgcgc atgccgcgga actcggcgtg tcccacgtgc
     3301 tgatcgaggc cgaggcacac atctccaaca ccatcttccg ctaccagaaa ggcaagcacg
     3361 tgatggacga gccgcgcgtg ctgccgctgc gcagcccgct gcagttcacc gccggcaagc
     3421 gcgaggcagt gctcgaggcc tggcagaacg gcctgcaggc gcacggcgcc gagctgctgc
     3481 tgaacagcga ggtcagcggc atctgcggac acaaaggcaa ctgcgaaatt caactcaggc
     3541 agggcgcgcc ggtgcgtgcc gagcacgtgg tgctggcgat cggcgtgcag ggtaatccgc
     3601 gcacgctggg cgtgccggga gaagatgcgc cgatcgtgca gtacacgctc gacgaccccg
     3661 ccgaataaga gggcgaacac atcatcgtca tcggcggcgg cgagatgcgc gcgttggtgc
     3721 gttcggcgat tgcccgcatc gaccgccacg agttttcgcc gcagcaaacc gcggcgctga
     3781 ccagcagcct gatctcgacc ggcatcgccg gccaataccg ggattaaatt gcggccgagc
     3841 aggcggtgat gggaataggc gcgctggtcg ccgcgtggaa cgcgatatgc aacccttcga
     3901 tccgcgcacc accgagctgg tcggcggtct gatggacact ctgttcgact cggtcgccga
     3961 cgaagagagt tgccaaccgg ccgaattctc ggcggtgcgg gcgaaactca aaagtggttt
     4021 atcgatccgc taacggcagg cttcaggcgt cgcgcccgac cacgtagcgg tcgcagaggt
     4081 tctccgccag acagtgcccc tggcgcaact gatctgcgga catgcgcgcc tcgataaacc
     4141 cgagcttgag gcgcgcgtat cgggaaccct ggattaccgc gagcttgcac caggcgtaag
     4201 ccctgggcag gctttgctcg acgccctggc cgcgcgcata catcgatgcc agccggtgct
     4261 gggcgcggcg cgagccattt tgcgcggctt gccgatacca tctgaaagcc tcgcaatagt
     4321 cccttcgcga tgcctggccc ttgtcgaata gctcgcccag ttcgaactgg gtcgtcgagc
     4381 tgcgttgctc cgcgtatatc accatacctg tgcccgctgc cggatctcgg tccagaattc
     4441 ggtgagtcta gacaaccccc gggcaattaa atgtgaagca tttatcacta tcttcgactc
     4501 cgcgggcccg cgcggcggaa tgcgccgcag cggccccgcc cccgctattg gcacggaaaa
     4561 gcgtcgatga aggaattcag cgcgctaccg gtggccggca attgcagttc ttcgacatgg
     4621 tctaccgcgt aacgcaggta aacctggcgc aggttgacga tattgatact ctcacccatg
     4681 cagaactgct gggcaatgtc gccccagcgg tgatgcgtct gcgacgcgtc gaccgcgccg
     4741 gcgagataaa atacgcaggc gagctggtgg accacttcct cactattgca cttgaccagc
     4801 aattgctcac cggtgaagaa acccgccttt gccgggctcg caccgaacag cagcgcggtg
     4861 ataagggttg ccgcgagcct catatgatgg ttctcctgtg gttgaaagcc acgtggcgcg
     4921 tcgtattgcg cggctgcgcg gcgtcagcga tagcatataa cagggggcga aaataatccc
     4981 ccggcttgca ccgctcgggc gctgcgttgc tttatcccgg cgggagaaat agtatatgtg
     5041 gatggtatgt tcagccccgg tgaggtcttg aaatgtcgtt ctccggtatt cgaatcgctg
     5101 tgctgattgc agcgctctcg ctgctctccg ggcccgccgg cgccgcttcc ggggtgttgt
     5161 tgctggccca ggccgatagc ggcggcgagc gccagggcaa gccgcgcctg atcgatttct
     5221 ggcagcccgg cgacaacggc cagcggatga atattcgtgg ccgcgtcacc agcctcgacg
     5281 gtacgccgct ggccggcatc ccgatctcga tccggcaagc caacggcgat ggtgaataca
     5341 ccgatcgcta ccgtggcacg ctgctgtcgg acgaacaggg gcgtttccag ttcgggtcgg
     5401 tgttacccgg taattacacc ggcgcgcggc acgtgcatat cgccgtctac accgacggct
     5461 acgagtactt cgacaccagc atcctgttca aggacgaccc caaccaggaa tcgcattacg
     5521 gctacggtac ggcgattttt ctcgaggagg ccacggtggg cggagaaacc atgctgttcg
     5581 ggcgcttcga catcatgctc acgccgatgt gatgcggcca gccgcagcag caacccgagt
     5641 cgatagatag caagccgatg aaaaaaatca ctaccctgtt gatcctgctg gctacgcttt
     5701 gccggccggc catcgcccag acgccgatca tcgccgacca cctggtgagc aacgacagcc
     5761 tgccgggcaa cgcgcacgaa ttctatttca cgcgcggcat ctacagcggc gagtacgacg
     5821 actacgacca gggcggccgc tgggcagtcg actatccgaa ggcggaccac cagttcctgg
     5881 tcgccctcaa gcggctaagc atcgtcgacg cctatgaatc cgataacgcg attacgctga
     5941 ccgatccccg gctgcgccgc tttccgctgc tctacgcggt cgaagtcggc tccatgtcgt
     6001 tgaacgaaga agaggtcgag gcattgcgcg attacctgct ggctggcggt ttcctggtgg
     6061 tcgacgactt ctggggcagt tgggcctggg agcagttcag cacgcagatg catcgcgtac
     6121 tccccgaccg cgcgatcgtc gaaatccc
//
LOCUS       DATCZV010000111         8223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_431894, whole genome shotgun sequence.
ACCESSION   DATCZV010000111 DATCZV010000000
VERSION     DATCZV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431894"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VIW27_02520"
     CDS             complement(<1..711)
                     /locus_tag="VIW27_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5738567.1"
                     /translation="MQPSKALLVKVWRGDASGGRLAEFDVPQQPAQTVLDVVTWIQRY
                     ADPGLSYRFACRVGMCGSCAMMVNGVPRWTCRTHVNKVLDDGRLTIEPLRNLPVIKDL
                     ACDMSEFFDKWQRAEGRFSGISTRASPVAEISPRDAARRNADAAIECINCAICYAACD
                     QVAWQPEYLGPAALNRAWSLQNDARDCRYESRLRAVTADGGCQQCHSQQGCAAHCPNT
                     LNPTASIAGLKRMATRRLL"
     gene            796..1671
                     /locus_tag="VIW27_02525"
     CDS             796..1671
                     /locus_tag="VIW27_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738568.1"
                     /translation="MNLRQLRSLSAIRKLGSFAAAGEHIGLSQSAISVQMQQLEQSLD
                     AALFERGSRPVTLTPEGARIAAVADEVLDRLAQIERIASGNEIPHSVTLGFVPTTVHY
                     LLPRVLEALREAWPELQVRVRSGLSGELSAAVARRELDYALLTAPVMEIPELEFTAVA
                     SEPFYAIGPVALAGIESDAELLRSMPYIAFNRRTWVGQHIAARLQQRGIHVSDAIEID
                     SLEAIENLVARGFGVSIVPRRLHDFVDTAKLVQIPFGSPVDTRQLVLVRHAARARSPF
                     DRALRQIFTGLPDQK"
     gene            1874..2101
                     /locus_tag="VIW27_02530"
     CDS             1874..2101
                     /locus_tag="VIW27_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738569.1"
                     /translation="MNDDTTLTGFDGLEGIDDTLTRTYTQIVSCVVGRNEVERRRQSL
                     RAKLEREQYERDQELQNSFVSTVRNLFSKIS"
     gene            2262..2816
                     /locus_tag="VIW27_02535"
     CDS             2262..2816
                     /locus_tag="VIW27_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5738570.1"
                     /translation="MLQIARRYVSIVDAVNYRIGRMMMYFIFVMIGILLWSSISKTFF
                     LPSLWTLEMAQFAMVTYYIMGGPYSIQLGSNVRMDLFYSSWTDRTRAWVDSCTILFLI
                     FYLGILLWGGFESTEYALQYGERSYSAWRPYMWPIKIIMCVGLALMLLQAIAELIKDL
                     FALRGEKIGRREEINPSGEVDRVE"
     gene            2806..4131
                     /locus_tag="VIW27_02540"
     CDS             2806..4131
                     /locus_tag="VIW27_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5738571.1"
                     /translation="MSNELIAILMFSSMMLMLMTGQRVFGAIGIVASVAALTLWGTGG
                     HDIPFTAAMKLMKWYPLLTLPMFIFMGYILSESRIADDLYRMFHVWMGGLRGGLAIGT
                     IGLMVLISAMNGLSVAGMAIGATIALPELLRRNYDKRMVTGVIQAGSSLGILVPPSVV
                     LVLYAMIARQPVGQLWLAGVLPGLMMAGLFVLYIAIRCHLQPQLGPAMPQAERDQVTL
                     KEKLRLLRAGMLPIVIFASMMVPFVNGWTSLVESSAIGALTAFAAAVIKGRMTRKVFE
                     TSIRNTLAISCMFMWIILAALGFGAVFDGLGAVDAIDDLFTEQLGLSPWMILVLMQLS
                     FLLMGTFLDDTAMLVIVAPLYVPLVDALGFNLIWYGVLYTITTQIAYMTPPFGYNLFL
                     MRAMAPPEITLRDIYGSILPFVGVMILALVLLMSFPEIALWLPNYVYSR"
     gene            4201..5244
                     /gene="dctP"
                     /locus_tag="VIW27_02545"
     CDS             4201..5244
                     /gene="dctP"
                     /locus_tag="VIW27_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY5738572.1"
                     /translation="MTTRRKFLKTGAGLAGTAALTAPAIHVSAKTTIKWRLQTYAGAP
                     LAAHVVKPAIDSFNKIAGNDMQIELYYADQLVPTGELFRAMQKRTIDAVQSDDDSMAS
                     PTEVTVFGGYFPFASRYSLDVPVLFNQYGLGEIWAAEYEKVGVKHLSAGAWDPCHFNT
                     KDPIRSLDDLKGKRVFTFPTAGRFLAQFGVVPVTLPWEDIEVAVQTGELDGVAWSGIT
                     EDYTVGWADVTDYFLTNNISGAWIGSFYANMDSYNALPDNLKELLKLTMDSSHYYRQW
                     WYWGGEANLRVNGTKMQLTSIPDAEWAKVEEAAIKFWEEIAAESPTKRKVVNIFRQYN
                     ADMQKAGRPYRYG"
     gene            5316..6683
                     /locus_tag="VIW27_02550"
     CDS             5316..6683
                     /locus_tag="VIW27_02550"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5738573.1"
                     /translation="MPGNLTMETLRERVANGQIDTVITAIADMQGRLMGKRFQAEFFI
                     DSAWEETHCCNYLVATDLEMYTVDGYTSTSWSAGYGDYIMKPDMSTLRVLPWLEGTAL
                     VLCDLLDHHTHALVPHAPRSVLKQQIARLQAMGMTSAVATELEFFVFRESYEELRDKG
                     YREMTTISPYNEDYHIFQTTKEENLMRALRNGLQGAGVGVENTKGEADPGQAEINVRY
                     SDALEMADNHCLVKNAVKEIAFQQGRAVTFLAKWSHDAAGSSSHIHQSLRSIDGEPVF
                     FDADAEYGMSATMRHYLAGLLQYASDNTFFLAPYVNSYKRFMAGTFAPTKAIWSLDNR
                     TAGYRVVGDGTRNVRIECRVGGSDLNPYLAIAAQLAAGIAGIENQLELEKEFKGDAYQ
                     ARRAREIPRTLAAAAQALKKSAMLRDAMGSDVIDHYVHAAEWELEDFYSKVTDYEVRR
                     GFERA"
     gene            6711..7856
                     /locus_tag="VIW27_02555"
     CDS             6711..7856
                     /locus_tag="VIW27_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007118459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY5738574.1"
                     /translation="MTITANWSYPTAIRFGAGRIAEIADACKAVGIEKPLLVTDRGLA
                     GLPITASTLALLEQAGLGNALFADVDPNPNENNLAAGIEAFNAGGHDGVIAFGGGSGL
                     DLGKLIAFMCRQTRPVWDFEDIGDWWTRADADAIAPNIAVPTTAGTGSEVGRAGVISN
                     SVTHEKKIIFHPKILPSIVICDPELTVGMPPAITAGTGMDAFAHCLEAYCSPHYHPMS
                     QGIAIEGMRLVKENLPRAYADGNDIEARAHMMSAAAMGATAFMKGLGAIHALSHPIGA
                     LYNSHHGTTNAVVMAPVLLFNREAIESRIERLAAYLGIAGGFDGFYEFVVELNASLGI
                     PANLTELGVTDPDLDWLVESALKDPSVGGNPIEMTAENTRKLFQACL"
BASE COUNT         1607 a         2562 c         2486 g         1568 t
ORIGIN      
        1 gagcaaacgc cgggtcgcca tgcgcttcaa gccggcgatc gacgcggtcg gattgagcgt
       61 gttgggacaa tgcgccgcgc aaccctgttg cgagtgacac tgctggcaac cgccgtcggc
      121 ggtgaccgcg cgcagccgcg actcgtacct gcagtcgcgg gcatcgttct gcaggctcca
      181 ggcgcggttg agcgctgccg gaccgaggta ttccggctgc cacgccacct gatcgcaggc
      241 ggcatagcag atcgcgcagt tgatgcattc gatggcggca tcggcgttgc ggcgcgcggc
      301 atcgcgcggc gaaatctcgg ctaccggcga ggcgcgtgtc gatatgcccg agaagcgccc
      361 ctcggcgcgc tgccacttgt cgaaaaactc gctcatatcg caggccaggt ccttgatcac
      421 cggcaggttg cgcaacggtt cgatggtcag gcggccgtca tcgagcacct tgttgacgtg
      481 cgtacgacaa gtccagcgcg gcacaccgtt gaccatcatc gcgcaggaac cacacatgcc
      541 gacgcggcaa gcgaaccggt agctgaggcc gggatcggca tagcgctgta tccaggtgac
      601 gacgtcgagg acggtttgcg cgggttgttg cggcacgtcg aattcggcca ggcggccgcc
      661 actggcgtca ccgcgccaga ccttgaccag aagggctttc gatggttgca tggcgccatg
      721 ttataacttt aataaaattg atgataatga attttttttg aattaaactt ttggtttttt
      781 aatgtaataa gggtaatgaa cctgcgccaa ctccgttcac tgtcggcaat tcgcaagctc
      841 ggcagtttcg ccgccgccgg cgaacacatc ggcctcagcc agtcggcgat cagcgtacag
      901 atgcagcagc tggagcaatc gctcgacgcc gcgctgttcg aacgcggcag ccggccggtc
      961 acgctgaccc ccgagggcgc gcgtatcgcc gccgttgccg acgaggtgct ggaccggctg
     1021 gcgcagatcg agcgcattgc cagcggcaac gaaatcccgc actcggtgac cctcggcttc
     1081 gtgccgacca ctgtgcacta cctgctgccg cgcgtgctcg aagcgctgcg cgaagcctgg
     1141 cccgagctgc aggtgagagt gcgctcgggg ctgtccggcg agttgtccgc ggcggtcgcg
     1201 cgccgcgagc tcgactacgc gttgttgacg gcaccggtga tggaaatacc cgaactcgag
     1261 ttcaccgcgg tggccagcga acctttttac gccatcggac cggttgcgct cgccggcatc
     1321 gagtccgatg ccgagctgct gcgctcgatg ccctatattg cgttcaaccg ccgcacctgg
     1381 gtagggcagc atatcgctgc acggctgcag cagcgcggca ttcacgtcag cgacgcgatc
     1441 gaaatcgatt cgctggaggc gatcgaaaac ctggtcgcgc gcggcttcgg tgtctccatc
     1501 gtgccgcgcc gcctgcacga tttcgtcgat accgcaaagc tcgtgcaaat cccgttcggc
     1561 tcgccggtgg atacccgcca actggtgctg gtgcggcatg cagcgcgtgc gcgctcaccg
     1621 ttcgaccgtg cgctgcggca aatcttcacc gggctgccgg accaaaagtg aggactggtt
     1681 cacgattcgt gtagcaacag cacaattcaa ctcgaataaa gcaccgaaat acgatacttt
     1741 tcgctctctc tgcaatggct ttcgaacaaa tcgtccgttt ttttgctgct tttaaatctg
     1801 tgcactggct caaggttttc tcagccacaa tccatagact gtgcagacca taagaaaata
     1861 tcttggagca tcgatgaacg acgacacgac cctgaccgga ttcgatggac tggaaggaat
     1921 cgacgacacg ctgacgcgca cctataccca gatcgtcagc tgcgtggttg gcagaaacga
     1981 agtcgagcgg cggcgccaga gcctgcgtgc caagctcgag cgcgagcagt acgagcgcga
     2041 ccaggaactg cagaattcct tcgtcagcac cgttcgcaac ctgttctcga aaatcagcta
     2101 atccaccaca cgccggccgg tcgctgtcgc gcatccggcc ctcgcctggt ccacgcctcg
     2161 ctgccgcttg actgcgttcg gcgataatga aaagatgccg gcaataacta gaacccggcc
     2221 ggccttggcc tcgttgttcg agcccgctat cctggaccgc aatgctccaa atcgccagac
     2281 gctacgtcag catcgttgac gccgtgaact accgcatcgg ccggatgatg atgtatttca
     2341 ttttcgtcat gatcggcatc ctgctgtggt cgtcgatctc caaaaccttc ttcctgccgt
     2401 cgctgtggac gctcgaaatg gcgcagttcg cgatggtcac ctactacatc atgggcggcc
     2461 cctactcgat tcagctcggc tccaatgtgc gcatggacct gttctacagc agctggaccg
     2521 accgcacccg cgcgtgggtc gattcctgca ccatcctgtt tctgattttt tacctcggca
     2581 tattgctgtg gggcggcttc gagagcaccg agtacgcgct gcagtacggc gaacgcagct
     2641 actcggcctg gcggccctac atgtggccga tcaagatcat catgtgcgtc ggcctggcgc
     2701 tgatgctgct acaggcgatt gccgaactga tcaaggacct gttcgcgctg cgtggcgaaa
     2761 agatcggccg gcgcgaggaa atcaacccgt cgggggaagt cgatcgtgtc gaatgaactg
     2821 atcgccatcc tgatgttctc gtcgatgatg ctgatgctga tgaccggtca gcgagtgttc
     2881 ggcgccatcg gcatcgtcgc ctcggtggcg gcgctgacac tgtggggcac cggcggtcac
     2941 gacattccgt tcaccgcggc gatgaagctg atgaagtggt acccgttgct gacgctgccg
     3001 atgttcatct tcatgggcta catactgtcg gaatcgcgta tcgccgacga cctctaccgc
     3061 atgttccacg tctggatggg cggattgcgc ggcgggctgg cgatcggcac catcggcctg
     3121 atggtactga tctcggcgat gaatggcctg tcggtggccg gcatggcgat cggtgcgacc
     3181 atcgcgctgc ccgaattgct gcggcgcaat tacgacaagc gcatggtgac cggggtgatc
     3241 caggccggct cgtcgctcgg tatcctggtg ccgccgtcgg tggtgctggt gctctacgcg
     3301 atgatcgcgc gccagccggt cggccagctg tggctcgccg gggtgctgcc ggggctgatg
     3361 atggccgggc tgttcgtgct ctacatcgcg atccgatgcc atttgcagcc gcagctcggc
     3421 ccggcgatgc cgcaggccga gcgcgaccag gtgacgctga aagaaaaact gcggctgctg
     3481 cgtgcgggca tgttgccgat cgtgatcttc gcgtcgatga tggtgccgtt cgtcaacggc
     3541 tggaccagcc tggtcgaaag ctcggcgatc ggtgcgctga ccgcattcgc ggcggcggtc
     3601 atcaagggcc gcatgacgcg caaggtgttc gagacttcga ttcgcaatac gctggcgatc
     3661 tcctgcatgt tcatgtggat catcctcgcc gcgctcggtt ttggcgcggt gttcgacggc
     3721 ctaggcgcgg tcgacgcgat cgacgacctg ttcaccgagc agctgggact gtcgccgtgg
     3781 atgatcctgg tgctgatgca gttgtcgttc ctgctgatgg gtacctttct cgacgatacc
     3841 gcgatgctgg taatcgtcgc accgctgtac gtgccactgg tggacgcgct cggtttcaac
     3901 ctgatctggt acggcgtgct ctacacgatc accacccaga tcgcctacat gacaccgcca
     3961 ttcggctata acctgttcct gatgcgcgcg atggcgccgc cggaaatcac cctgcgcgat
     4021 atttatggat cgattctgcc gttcgttgga gttatgatac tggcgctggt tctactaatg
     4081 agttttccgg aaattgccct gtggctgccg aattatgtct actcgcgctg acccgggtgc
     4141 ttcacggaaa cccgataaca agtcgaaacc gataattcaa ccaacaaaga gaggcatccg
     4201 atgacaacca gacgtaagtt cctcaaaacc ggcgccgggc tggccggcac cgcggcgtta
     4261 acggcgccgg cgatccacgt atccgcgaaa accaccatca agtggcgcct gcaaacctac
     4321 gccggcgcgc cgctcgcagc ccacgtggtc aagcccgcga tcgactcgtt caacaagatc
     4381 gccggcaacg acatgcaaat cgaactgtat tacgccgacc agctggtacc gaccggcgaa
     4441 ctgtttcgcg cgatgcagaa acgcaccatc gacgcggtgc agtccgacga tgattcgatg
     4501 gcgtcgccga ccgaagtaac cgtgttcggc ggctacttcc cgttcgcgag ccgttactcg
     4561 ctcgatgtac cggtgctgtt caaccagtac ggtctcggag agatctgggc cgccgagtac
     4621 gagaaagtcg gcgtcaagca cctgtctgcc ggcgcctggg atccgtgtca tttcaatacc
     4681 aaggacccga ttcgcagcct cgatgacctg aagggcaagc gcgtgtttac cttcccgacc
     4741 gccggccgct tcctggcgca gttcggcgtg gtgccggtaa ccctgccatg ggaagacatc
     4801 gaggtcgcgg tgcagaccgg cgaactcgac ggcgtggcct ggtccggtat caccgaggat
     4861 tacaccgtcg gctgggcgga tgtaaccgac tacttcctga ccaacaacat ctccggcgcg
     4921 tggatcggtt ccttctacgc caacatggac agctacaatg cattgcccga taacctcaag
     4981 gaactgctca agctgaccat ggacagctcg cactactacc gccagtggtg gtactggggc
     5041 ggcgaagcca acctgcgtgt caacggcacc aagatgcagc tgacctcgat tccggacgcc
     5101 gaatgggcca aggtcgagga agccgcaatc aagttctggg aagagatcgc cgccgagtcg
     5161 ccgaccaagc gcaaggtcgt caacatcttc aggcagtata acgcggacat gcaaaaggcc
     5221 ggccggcctt accgttacgg ctagcgccgg gtccgatgaa accgcggggg cgtaatcctt
     5281 acgcccccgt ttttcggcta agcgagtaaa acactatgcc aggcaatctc accatggaga
     5341 ccctcaggga gagggtcgcc aacggacaaa tcgataccgt aatcaccgcg attgcggaca
     5401 tgcagggacg cctgatgggc aaacgttttc aggccgagtt tttcatcgac agcgcctggg
     5461 aagagactca ttgctgcaac tacctggtcg ccaccgacct cgagatgtat accgtcgacg
     5521 gttacacgtc gaccagctgg tcggccggtt acggcgacta catcatgaag cccgacatgt
     5581 ccacgctgcg ggtgctgccg tggctcgagg gcacagcgct ggtattgtgc gacctgctcg
     5641 accaccatac ccatgcgctg gtaccgcacg caccgcgctc ggtgctcaag cagcagatag
     5701 cccgtctgca ggcgatggga atgacctcgg cggtggctac cgaactcgag ttcttcgtgt
     5761 ttcgggaaag ttacgaggaa ttgcgcgaca agggctaccg cgagatgacg accatcagcc
     5821 cgtacaacga ggattaccat attttccaga ccaccaagga agaaaacctg atgcgcgcgc
     5881 tgcgcaacgg gctgcagggc gccggggtcg gcgtcgagaa taccaagggc gaagccgatc
     5941 cggggcaggc ggaaatcaac gtgcgctaca gcgacgcact ggaaatggcc gacaaccatt
     6001 gcctggtaaa gaacgcggtc aaggaaatcg cctttcaaca gggccgcgcg gtaacctttc
     6061 tcgccaagtg gagccacgac gcggccggtt cgtcgagtca cattcaccag tcgctgcgca
     6121 gcatcgacgg tgagccggtg tttttcgacg ccgacgccga gtacggcatg tccgctacca
     6181 tgcgccacta cctggccggg ctgctgcagt acgccagcga caacacgttt tttctcgcgc
     6241 cctacgtcaa ttcctacaag cgcttcatgg ccggcacttt cgcgccgacc aaggcgatct
     6301 ggtcgctcga taatcgcacc gcgggttatc gcgtggtcgg cgacggcacg cgcaatgtgc
     6361 gcatcgagtg ccgcgtcggc ggcagcgacc tgaatccata cctggcgatc gccgcgcaac
     6421 tcgcggccgg catcgccggc atcgagaacc agctcgagct ggaaaaggaa ttcaagggcg
     6481 atgcttacca ggcgcgccgc gcgcgtgaaa ttccgcgcac cctcgccgcc gccgcccagg
     6541 cgttgaaaaa atccgccatg ctgcgcgacg cgatgggctc ggacgtgatc gatcactacg
     6601 tgcacgcggc cgagtgggag ctggaagatt tttacagcaa ggtgaccgac tacgaggtca
     6661 ggcgcggctt cgaaagagcc tgagccgaca acccataacg agacgacgag atgacaatta
     6721 ccgccaactg gtcctatccc acggcgatcc gcttcggtgc gggacgcatc gccgaaatcg
     6781 ccgacgcctg caaggctgtc ggcatcgaaa agccgctgct ggtcaccgac cgcgggctcg
     6841 ccggcctgcc gattaccgcc agtacgctag cattgctcga acaggccggg ctcggcaacg
     6901 cactgttcgc cgacgtcgac ccgaatccca acgaaaataa cctggcggcc ggcatcgaag
     6961 ccttcaacgc cggcggccac gacggcgtca tcgcttttgg cggcggttcc gggctcgatc
     7021 tcggcaagct catcgctttc atgtgccggc agacgcggcc agtctgggat ttcgaagata
     7081 tcggcgactg gtggacgcgc gccgatgccg acgcgatcgc gccgaacatc gccgtgccga
     7141 ctaccgccgg caccggctcc gaggtcggcc gcgcgggtgt catctccaat tccgtgaccc
     7201 acgagaagaa aattatcttc cacccgaaga tcctgccgtc gatcgtgatt tgcgatcccg
     7261 agttgaccgt cggcatgccg ccggcgatca ccgccggcac cggcatggat gcgttcgcgc
     7321 attgcctgga agcctactgc tcgccgcact accacccgat gagccagggc atcgccatcg
     7381 agggcatgcg cctggtcaag gaaaacctgc cgcgcgccta cgccgacggt aacgatatcg
     7441 aggcccgagc gcatatgatg tcggcggcgg cgatgggcgc gaccgcgttc atgaaggggc
     7501 tgggcgcgat tcacgcgctg tcgcacccga tcggcgcgct gtacaacagt caccacggca
     7561 ccaccaacgc cgtggtcatg gcgccggtac tgctgttcaa ccgcgaggcg atcgagagcc
     7621 gtatcgagcg cctcgccgcc tatctcggca tcgccggcgg ctttgacggc ttttacgaat
     7681 tcgtcgtcga actcaacgcg tcgctgggca ttccggccaa cctgaccgaa ctcggggtca
     7741 ccgatccgga cctcgactgg ctggtcgaat cggcgctcaa ggatcccagc gtcggcggca
     7801 atccgatcga gatgaccgcg gaaaacacgc gcaagctgtt ccaggcctgc ctgtaggcag
     7861 cccctggaag cctgtgtata acaggtagat cgcgaggata aagctcaggg acaggccgcc
     7921 gataatccgg cgcgccagcg acatcaggtc gagatcgatt gtttcgctca ttttttgccc
     7981 tcgtggtcga agactgcaaa agctatgcag tcaatacccc gatcgcttag attttggtca
     8041 atcaacattg attaatttgt ggcattagca gaaccgcacc ttcaaacccc ggtcgagctt
     8101 gtccttcgaa tcattcccgc gtatgcgtcg gtccggcgga ccggaatcag atagcgtgcc
     8161 gccactacaa gacccccgct tgcgcggggg tgactctgga tagaggcgcc gttgagagat
     8221 ccc
//
LOCUS       DATCZV010000112         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_220098, whole genome shotgun sequence.
ACCESSION   DATCZV010000112 DATCZV010000000
VERSION     DATCZV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220098"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VIW27_02560"
     CDS             <1..788
                     /locus_tag="VIW27_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5738575.1"
                     /translation="IGLLLILPLCAPAAPRQSLVPGGVAIIALDGETEPQYRFRGKPV
                     LISEIDGVTSAVIGLPINLEPGEYHIESSAGSRVHKRYFEVVGKQYTTQYITIENDRK
                     VNPYASDMKRILAEKKRKQKARDHYSRGAVDVDFLLPVEGTNTGSYGRRRFFNGQPRS
                     PHSGMDIAADTGTPVQNPSDGTVIELGDFFFSGKLVYIDHGQGMISLFAHLSEIDVEL
                     GQRVAKGEIIGKVGATGRVTGPHLHWSLGLNGSWVDPALFLPQ"
     gene            complement(894..1439)
                     /locus_tag="VIW27_02565"
     CDS             complement(894..1439)
                     /locus_tag="VIW27_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738576.1"
                     /translation="MLVLTAVPLAQASDFEAALSKETAQFTFRSDSSVVGWGGADLGL
                     GFFYNEESDVIGHFSLLQMRQPSQQNPLTFGVGVKAYLGVLDNIDADVFALAIGGQLR
                     YTIAGTMPMAIYLEGHYAPEITSFADIDKVIDYSLGFQVEALPQTIAFVGVRRFEFEL
                     DNGADYDADDSNIHIGIRLTF"
     gene            complement(1793..>2086)
                     /locus_tag="VIW27_02570"
     CDS             complement(1793..>2086)
                     /locus_tag="VIW27_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738577.1"
                     /translation="RHKMIRMAVVANRVRKHTLMYHSLERFLFSLNIPFISSLRDTQL
                     YARAIEMGVGVQEVSLSRNRTDREQWAPIFRWIETPIVSESTPVTRLQGHFEN"
BASE COUNT          419 a          658 c          648 g          361 t
ORIGIN      
        1 tgatcggcct gctgctgatc ctgccgctgt gcgcgccggc cgcgccgcgc cagtcgctgg
       61 tgcccggcgg cgtcgcgatc atcgcgctcg acggcgagac cgagccgcaa taccggtttc
      121 gcggcaaacc ggtgctgatc agcgagatcg acggcgtgac cagcgcggta atcggcctgc
      181 cgatcaacct cgagcccggc gagtaccata tcgagtccag cgccggcagc cgcgtgcaca
      241 agcgctattt cgaggtcgtc ggcaagcagt acaccacgca atacatcacc atcgagaacg
      301 atcgcaaggt caatccctac gccagcgaca tgaagcgcat tctcgccgag aagaagcgca
      361 agcaaaaggc gcgggatcat tactcgcgcg gtgcggtcga tgtcgatttc ctgctgcccg
      421 tcgagggcac caacaccggc agctacggtc gccggcgttt tttcaacggt cagccgcgca
      481 gccctcacag tggtatggac atcgccgccg ataccggcac cccggtgcaa aaccccagcg
      541 atggcacggt aatcgaactc ggcgatttct ttttcagcgg caaactcgtc tacatcgacc
      601 acggccaggg catgatctcg ctgttcgcgc acctcagtga aatcgacgtc gagctcgggc
      661 agcgggtcgc caagggcgaa atcatcggca aggtcggcgc caccggccgg gtgaccggac
      721 cgcacctgca ctggagcctc ggcctcaacg gtagctgggt cgacccggcg ctgttcctgc
      781 cgcaatagcc gcttcgcagg gcgcccgcta cgcggctcgc gagggcgtca accaccgacg
      841 acgggccgtg cggcaaccgc gagcacccgc tggcggggcc agttgcccgc cgactaaaat
      901 gtcagccgta tgccgatgtg gatattgctg tcgtcggcgt cgtagtcggc accgttatcg
      961 agctcgaatt caaagcggcg cacgccgacg aaggcaatgg tctgtggcag cgcttcgacc
     1021 tgaaaaccga gcgagtaatc gataaccttg tcgatatccg caaagctggt gatttccggc
     1081 gcgtagtggc cttcgagata gatcgccatc ggcatggtac cggcaatggt ataacgcagc
     1141 tggccgccga tcgcgagggc gaacacgtcg gcatcgatat tgtccagcac gccgaggtaa
     1201 gccttgactc cgaccccgaa ggtcagcggg ttttgctgcg acggctggcg catctgcaac
     1261 aggctgaaat ggccgatgac atcgctctcc tcgttgtaaa aaaatccaag gccgaggtcc
     1321 gccccgcccc agccgacgac gctggaatcg gaacgaaagg taaactgcgc ggtttccttg
     1381 ctgagcgcag cttcgaaatc actggcttgg gcgagcggca cggccgtgag aacgagcatg
     1441 ccgagagtaa ttgaaggtat cagtttcatg gcgcaaccgt gggaaaaatc aggcgcaatc
     1501 atagcacagg cttttcccgg gttgcgcact ccacagcgcg aaatggaaat agttaactgg
     1561 ccgacgcaca agccggcggc ctcggcgccg acagcatcct ggtgcctggt cttgcatcgc
     1621 tatagcgcgc tgctgcgcac ctaccgcggc cgaggctgca gcagccggca ccgaacccgg
     1681 tctggagccc gtcacaccag gcgccatacc ggtctcgctt gcgataagct cgagcgaatt
     1741 cgatgggcta gatttgtgga gcggttgcgg accggccgcc ggaatccgcg ggctagttct
     1801 cgaagtggcc ctgcagacgc gtaaccggcg tgctctcgct gactatcgga gtctcgatcc
     1861 agcggaatat cggcgcccat tgctcgcggt cggtgcgatt gcgcgacagc gatacttcct
     1921 gcacgccgac gcccatttcg atcgcgcgcg catacagctg ggtgtcgcgc aggctggaaa
     1981 taaacggaat gttgaggctg aacagaaacc gctccagcga atggtacatc agggtgtgct
     2041 tgcgcacccg gttggcgacc accgccatgc gaatcatctt gtggcg
//
LOCUS       DATCZV010000113         3566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_590403, whole genome shotgun sequence.
ACCESSION   DATCZV010000113 DATCZV010000000
VERSION     DATCZV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590403"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1686
                     /locus_tag="VIW27_02575"
     CDS             334..1686
                     /locus_tag="VIW27_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5738578.1"
                     /translation="MNAPLSNSDLIHKDQQHMIHPLHHNPAHAGGKVWVRAEGSHLFD
                     ADGNRYIDGLSCLWNVSCGHGRRELADAAHQQMLKLAFCSSYNGSSNPPAIELAERLA
                     ERCYSNINNFYFTSGGGESTDSNFKLARYYWKAKGKPEKTKVISRIWGYHGVTFAAMC
                     ATGIAPYWAAFEPRIPGFSHIPAPDPYHYPEPEDGSSPGIAMANELEKEILRQGAETV
                     AMFIAEPVQGAGGVIVPPDDYFPRIREICDQYEVLFVADEVITGFGRTGKLFALEHYG
                     VEPDLMQFAKSITSGYLPLGGIGISDEVAETIRGGDKPWMHAYTYSGHPTTCAVGLAT
                     LDFIEREGVVAQAAEKGAYLLERLHAELDAHPHVGNIRGKGMMCGVELVEDRASRSWY
                     PPEWGVGPKMTKAMIDNGVFTRMRNEVVCIAPPLTTEQTTLDELVAGVRDAVVSVFGD
                     "
     gene            complement(1703..2788)
                     /locus_tag="VIW27_02580"
     CDS             complement(1703..2788)
                     /locus_tag="VIW27_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014107853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5738579.1"
                     /translation="MLSELQARLAPYLQLLGGNPWLQAAAVVIVSVLLAWLFDRVVSA
                     LLRKLAARTRIAFDDLLIGYLHGPVQTSVILLGLALAADLLEIPDPYDFIIFSTLLTI
                     AYVVWMLFLLRVVRSVLAFVARDARRVSVLHAQTLPLFDNLAMIFVVAIGIYMVFSAW
                     GVDMTAWLASAGIIGIAVGFAAKDTLANLFSGVFILADAPYKIGDYVVLDSGERGEVT
                     HIGIRSTRIRTRDDVEVTVPNAIMGNTKIINESGGPYEKYRIRIKVGVAYGSDIDRVE
                     EILLELAAVNELVCEEPAARVRFRAFGASGLDFELLCWVDQPAQRGLVTHHLLRAIYQ
                     RLNAEGIEIPYSKHDIYIKQMPSRSPE"
     gene            complement(2782..>3566)
                     /locus_tag="VIW27_02585"
     CDS             complement(2782..>3566)
                     /locus_tag="VIW27_02585"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY5738580.1"
                     /translation="EQAWRSEQQVRSGLLEIWKVMRTCVRNGCRNEGILPGGMKVRRR
                     AADLYRRLGSQPHITYKDPLNTLDWVNLWALAVNEENAAGGRVVTAPTNGAAGIIPAV
                     LHYYQHFFPAADDDGIVRFLLTAGAIGILYKENASISGAEVGCQGEVGSACSMAAGAL
                     TEVLGGSPEQVENAAEIGMEHNLGLTCDPVGGLVQVPCIERNAMASVKAINAARIAMR
                     GDGKHFVSLDKVIKTMRDTGADMKTKYKETARGGLAVNIIEC"
BASE COUNT          660 a         1131 c         1145 g          630 t
ORIGIN      
        1 cgtgagcgcg gacattcccg gtgaactcgc ctgcgcgcag gccggccatc cgcaggtcct
       61 gattcggctg tcgcagtacc tcgggcagaa cgaagcgatg gcggagctgg tgctgcgctg
      121 ttcacggctg gccgccgagc cgcgcggcta acccgctgcc catcgcggtt gcactggcgg
      181 cactggccgt ccgtccctct gctgtcgcct cgacgccggg ctcgtcggca ccgctcctcg
      241 tgatcgccga ataacgggtt taatagccag gcggaaagcc tataatacgc tgacggccga
      301 tctcccggca gccacgagca cagcgaggcc actatgaatg cccccttgag taatagcgat
      361 cttatccaca aagaccagca gcacatgatt cacccgctgc atcacaaccc ggcccatgcc
      421 ggcggcaagg tctgggtcag ggcggaggga tcgcacctgt tcgacgccga cggcaaccgg
      481 tacatcgacg gcctgtcatg cctgtggaac gtcagttgcg gacacgggcg ccgggagctg
      541 gccgatgccg cgcatcagca aatgttgaag ctggcgttct gttcgagcta caacggcagt
      601 tccaatccac cggcgatcga actcgccgag cgcctcgcgg agcgctgcta ctcgaatatc
      661 aacaattttt atttcacgtc gggcggcggt gaatccaccg acagcaattt caagctcgcg
      721 cgctactact ggaaagccaa gggaaaacct gaaaaaacca aggtcatcag ccgcatctgg
      781 ggctatcacg gggtcacctt tgcggctatg tgcgcgaccg gcatcgcgcc ttactgggcc
      841 gcgttcgagc cgcgcattcc gggtttcagc cacatacccg cgcccgaccc gtaccactat
      901 ccggagcccg aggacggttc ttcgccgggt atcgcgatgg ccaacgaact ggaaaaggag
      961 attctcaggc agggcgcgga gaccgtcgcc atgtttatcg ccgagccggt gcagggtgcc
     1021 ggcggcgtaa tcgtaccgcc ggacgattat tttccgcgca ttcgggaaat ctgcgaccag
     1081 tacgaagtat tgttcgtcgc cgacgaggtc atcaccggct tcggccgtac cggcaagctg
     1141 ttcgcgctcg agcattacgg cgtcgagccg gacctgatgc agttcgccaa gtcgatcacc
     1201 tcgggctacc tgccgctggg cggcatcggc atcagcgacg aggtcgccga gaccattcgc
     1261 ggcggcgaca agccgtggat gcacgcctat acctatagcg gtcacccgac gacctgcgcg
     1321 gtgggcctgg cgacgctcga tttcatcgag cgcgagggcg tggtcgcgca ggccgccgag
     1381 aaaggtgctt acctgctcga acgcctgcac gccgagctcg acgcgcaccc gcacgtcggc
     1441 aatattcgcg gcaagggaat gatgtgcggc gtcgaactgg tcgaggatcg tgccagcagg
     1501 tcctggtatc cgccggaatg gggcgtcggg ccgaaaatga ccaaggcaat gatcgacaac
     1561 ggcgtgttca cgcgcatgcg caacgaagta gtgtgcatcg cgccgccgct gactaccgag
     1621 cagaccacgc tcgacgaact ggtcgccggc gtgcgcgacg ccgtggtcag cgttttcggc
     1681 gactagggag cgaaaatccc gcttactcgg gactccgcga aggcatctgc ttgatgtaga
     1741 tgtcgtgctt ggagtagggg atctcgatgc cctcggcatt cagacgctgg tagatcgcgc
     1801 gcagcagatg atgcgtcacg aggccgcgct gggccggttg atcgacccag cacagcagct
     1861 cgaagtcgag gccggaggca ccgaaggcgc gaaaccgcac cctggcggcg ggttcctcgc
     1921 aaaccagttc gttgacggcc gcgagttcca gcagtatttc ctcgacgcgg tcgatgtcgc
     1981 tgccataggc gacgccgacc ttgatccgaa tacgatattt ttcgtagggg ccgccggact
     2041 cgttgatgat cttggtattg cccatgatcg cgttgggcac cgtgacctcg acatcgtcgc
     2101 gggtcctgat acgcgtgctg cggatgccga tgtgggtcac ctcgccacgc tcgccggaat
     2161 ccagcaccac gtagtcgccg atcttgtagg gcgcgtcggc gaggatgaat accccggaga
     2221 acaggttggc gagcgtgtcc ttggcggcga agccgaccgc gatgccgatg atgcccgccg
     2281 acgccagcca cgcggtcatg tcgacgcccc aggccgagaa caccatgtaa atgccgatcg
     2341 cgacgacgaa gatcatcgcc aggttgtcga acagcggcag ggtctgcgcg tgcagcacgg
     2401 agaccctgcg cgcgtcgcgc gcgacgaagg ccagcaccga tcgaaccacc cgcagcagga
     2461 acagcatcca gacgacgtag gcgatggtca gcagtgtcga aaagatgatg aaatcgtagg
     2521 gatccgggat ttccagcagg tcggcggcga gcgccaggcc gagcaggatg acgctggttt
     2581 gcaccgggcc gtgcaggtaa ccgatgagca ggtcgtcgaa agcgatgcgc gtgcgtgccg
     2641 cgagtttgcg cagcagcgcg gataccacgc ggtcgaacag ccaggccagc agcaccgaga
     2701 cgatgaccac cgcggctgcc tgcagccacg ggttgccgcc gagcagttgc aggtagggcg
     2761 cgagccgcgc ctgtaactcg ctcagcattc gataatattt accgccagcc ccccgcgtgc
     2821 ggtctccttg tacttggttt tcatatcggc gccggtatcg cgcatcgtct tgatgacttt
     2881 gtcgagcgag acgaagtgct tgccgtcgcc gcgcatcgcg atgcgtgccg cgttgatcgc
     2941 cttcaccgaa gccatcgcat tgcgctcgat gcagggcacc tgcacgaggc cgccgaccgg
     3001 atcgcaggtc agtccgaggt tgtgctccat gccgatttcg gccgcgttct cgacctgctc
     3061 ggggctaccg ccgagaacct cggtgagcgc gccggcggcc atcgagcagg cactgccgac
     3121 ctcgccctgg cagccgacct cggcgcccga aatcgatgcg ttttccttgt agagaatgcc
     3181 gattgcgccg gccgtcagca aaaagcgcac gatgccgtcg tcgtcggcgg ccgggaagaa
     3241 atgctggtag taatgcaata ccgccggaat gatgccggcc gcgccgttgg tcggcgcggt
     3301 gaccacgcgt ccgccggcgg cgttctcttc gttgaccgcg agtgcccaca ggttgaccca
     3361 gtcgagtgtg ttcagcgggt ccttgtaggt aatgtgcggc tggctgccga gccggcggta
     3421 gagatccgcc gcgcgtcgcc tgactttcat gccaccgggt aatataccct cgttgcggca
     3481 gccgtttctg acgcaggtac gcatcacttt ccagatctcc agcaggccgc tgcggacctg
     3541 ctgctcgctg cgccaggcct gttcgt
//
LOCUS       DATCZV010000114         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_167198, whole genome shotgun sequence.
ACCESSION   DATCZV010000114 DATCZV010000000
VERSION     DATCZV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167198"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VIW27_02590"
     CDS             complement(<1..520)
                     /locus_tag="VIW27_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5738581.1"
                     /translation="MDYETYHLTNFELQSGAVLPDARLAYKTYGKLNAARDNVVVLPT
                     FYTGTHLRNEGFFGAGRAIDPARHFVVSINLFGNGISSSPSNTPAPFAGADFPRVTLF
                     DNVRAQHSLLSDGLGVERIALVSGWSMAGCQSFQWGAQYPQMVDAILPFCASAKTSEH
                     NIVFLEGVKAALT"
     gene            complement(703..2115)
                     /locus_tag="VIW27_02595"
     CDS             complement(703..2115)
                     /locus_tag="VIW27_02595"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5738582.1"
                     /translation="MSNTYYEKMEQSFPSKARIRKEQKRLADAGVKYVLSCWIDLLGV
                     PKTKPVPLADFEPLCLGKGPQFAVHSVSFVPELGPADSDQIPVPDLDTLVICPWDPTC
                     AWVFADLWWEGKPYNLCPRQALKRAIRDAEKQGYAMMAGIEPEFMTMQWDENGLPVKA
                     FDDDPPDGIRPRRQAFGYDIEYSIDAMPFLGQLIDILNGLGWGLHDVVAEGAYSQFEL
                     DFHYADVLTMADRLVFLRVLLKEIAKQHDMFVTFMPKPTTGDWRSGAHMNTSLVKVKQ
                     PGKNIYQGTGGKFSQAAYHAVGGMLKHGAAITAVACSTVNSYNGLVPKVAGFEGGVYT
                     WAPTHITYGHNNRSAMLRLPQNRFCIENRAADMCMNPYLGLGMSLAASVDGIVNKIDP
                     GPSADEDLYLMDTKKQKKLTALPRHLLEATEQLGNSSLAKQVLGEQMLSSFVAYKVDE
                     WERYHQVTTDWELKEYLRLF"
BASE COUNT          390 a          650 c          687 g          433 t
ORIGIN      
        1 cggtcaacgc ggccttgacc ccctcgagga acacgatgtt gtgctcggaa gttttcgccg
       61 aggcgcagaa cggcagaatc gcgtcgacca tctgcgggta ctgtgcgccc cactgaaaac
      121 tctggcagcc ggccatcgac cagccgctca ccagcgcgat acgctcgacg ccgagcccgt
      181 cgctcagcag cgagtgctgc gcgcgcacgt tatcgaacaa ggtgacccgc ggaaaatccg
      241 cgccggcaaa tggcgccggc gtgttgctcg gcgaggatga aatgccgtta ccgaacaggt
      301 tgatcgagac cacgaaatgc cgcgccgggt cgatcgcgcg tcccgcgccg aaaaatccct
      361 cgttgcgcaa gtgggtcccg gtgtaaaagg tcggcagcac gacgacgttg tcgcgcgccg
      421 cgttgagctt gccgtaagtc ttgtaggcga ggcgcgcatc gggcaacacg gcacccgatt
      481 gcagttcgaa atttgtgaga tggtaggtct cgtagtccaa tcaactggtc cgatggttat
      541 tcttcagcgg cgcgagcgcc gcagttttcg ggtcatcccc gcgcaattct caaagtcatc
      601 cccgcgtaag cggggatctt gtaagtaagc catgataggg gattcctgct taggcgggaa
      661 tgacgccgtc gaatgcgcgg gctccgcttc gaaaactcgg gttcagaaca gccgcaggta
      721 ttccttcagt tcccagtcgg tcgtgacctg gtggtagcgt tcccattcgt cgaccttgta
      781 ggcgacgaac gatgacagca tctgttcgcc gagcacttgt ttcgcgaggc tgctgttgcc
      841 gagctgctcg gtggcttcga gcaggtggcg cggcagcgcg gtcagcttct tctgcttctt
      901 cgtgtccatc aggtacaggt cttcgtcggc cgacgggccg ggatcgatct tgttgacgat
      961 gccgtcgacc gaggcggcca gcgacatgcc gagcccgagg tagggattca tgcacatgtc
     1021 ggcggcgcgg ttctcgatgc aaaagcggtt ttgcggcagg cgcagcatcg ccgaacggtt
     1081 attgtggccg taagtgatgt gtgtcggcgc ccaggtgtag acgccgccct cgaagccggc
     1141 caccttgggc accaggccgt tgtacgagtt gaccgtgcta caggccaccg cggtgatcgc
     1201 cgcgccatgc ttgagcatgc cgccgaccgc gtggtatgcg gcctggctga acttgccgcc
     1261 agtgccctgg tagatgttct tgcccggctg cttgaccttg accagcgaag tgttcatatg
     1321 agcgcccgag cgccagtcgc cggtggtcgg cttgggcatg aaggtcacga acatgtcgtg
     1381 ctgcttggcg atctccttca gcagcacgcg caggaacacc agccggtcgg ccatcgtcag
     1441 cacgtcggcg taatggaagt cgagttcgaa ctgtgaatag gcgccctcgg cgaccacgtc
     1501 gtgcaggccc cagccgagtc cgttgagaat atcgatcagc tggccgagga atggcatcgc
     1561 gtcgatcgaa tactcgatgt cgtagccaaa agcctggcgg cgcgggcgta taccgtcggg
     1621 cggatcgtcg tcgaaagcct tcaccggcag gccgttttca tcccactgca tggtcatgaa
     1681 ctccggttcg atgccggcca tcatcgcgta gccttgcttt tcggcatcgc gaatcgcgcg
     1741 cttcagtgcc tgccgcgggc acaggttata gggtttgcct tcccaccaca ggtccgcgaa
     1801 cacccacgcg caggtcggat cccacgggca gatcaccagc gtgtcgagat cgggcaccgg
     1861 gatctgatcg gagtcggccg ggccgagctc gggcacgaag gaaaccgagt gcaccgcgaa
     1921 ctgcggaccc ttgccgagac acaggggttc aaaatcggcg agcggtaccg gcttggtctt
     1981 gggcactccg agcagatcga tccagcagga cagcacgtac ttgaccccgg cgtcggccag
     2041 gcgtttctgc tccttgcgaa tgcgcgcctt gctgggaaag gattgctcca ttttttcgta
     2101 gtaggtgttg ctcatcgatg actcctcggt tgtgtttgta agctctgcat cagcgcaccc
//
LOCUS       DATCZV010000115         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_220297, whole genome shotgun sequence.
ACCESSION   DATCZV010000115 DATCZV010000000
VERSION     DATCZV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220297"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1797)
                     /locus_tag="VIW27_02600"
     CDS             complement(412..1797)
                     /locus_tag="VIW27_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY5738583.1"
                     /translation="MTSSLVTVSYLGAAALFILALGGLSNNETARRGNLFGMIGMAIA
                     VAVTIVAFVNRHYEVLVGLMAAGGIIGFILARRVKMTQMPELVAILHSLVGLAAVLIG
                     FANFMDTSISYIGVEKTIHEIEIYIGIAIGALTFTGSVIAYGKLSGLIGGAPMMLPGR
                     HWLNLAIFIAVLVIGAKFVGAEDHHVATLLLLAMTVLALLFGIHMVAAIGGADMPVVI
                     SMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRHFISVIAGGFG
                     TTSGGVSAGYEGEVQTTTVEEVAQMLNNANKVMVVPGYGMATAQAQHAVAEIAKTLIK
                     KGVNVRFGIHPVAGRMPGHMNVLLAEARVPYDIVFEMDEINDDFPNIDVSIVVGANDT
                     VNPLAQEDASSPIAGMPVLEVWKGETTIVLKRGMAAGYAGVDNPLFVKENTRMLFGDA
                     RDSLEALAGAL"
     gene            complement(1797..>2321)
                     /locus_tag="VIW27_02605"
     CDS             complement(1797..>2321)
                     /locus_tag="VIW27_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEY5738584.1"
                     /translation="MEDDVLRGATVVKDGEITWPPPPIAVSAERKPSYGAQEIKPEEQ
                     EKAPDAVIQSSAVADFFKSAWFPVIAAGLALLGLGQVAPPSFMSHFTVFVLSCFIGYM
                     VIWNVTPSLHTPLMSVTNAISSIIILGALTQISSPSGLIYLLAAIAVFITCINIVGGF
                     AVTQRMLRMFRGDA"
BASE COUNT          479 a          721 c          707 g          414 t
ORIGIN      
        1 gctattcagc cgcgcagcag cccaaaacaa aaaaaagcag ccttgcggct gctttaaaat
       61 ccgggcccgg cgcactaacc gaaccctggt gcaacggacc gcgccgatga aaccctgaaa
      121 cgcggatccg tggttcctcg ccgcgagcgg cgtgggcacc gggaaaacat acaatcacta
      181 agcacttttg atggagggtt ttcccttacc tggatattgt gcaccaacgg caccgcgcgg
      241 gaatccccca aacaggttaa atttctagga aattcagtga tttagcgtca aaaaactgac
      301 ggtgacaggg ttttttacgg gctcgcggcg gcgcgccatc gaaaatcgcc gagccgccgg
      361 cagcggcacc gcggcggaaa gaatccgccg cgcaacgatc ctgggaacgg ctcagagtgc
      421 cccggccaga gcttcgaggc tatcacgcgc atcaccgaac agcatgcgtg tgttctcctt
      481 gacgaacagc ggattgtcga cgcccgcgta gccggcggcc ataccgcgtt tgagtacgat
      541 cgtggtctct cccttccaga cctcgagcac cggcatgccg gcgatcgggc tgctggcgtc
      601 ttcctgggcc agcggattga cggtgtcgtt ggcgccgacc acgatcgaca cgtcgatatt
      661 cgggaagtcg tcgttgattt cgtccatctc gaagacgatg tcgtagggca ccctggcctc
      721 ggcgagcagc acgttcatgt gcccgggcat gcgcccggcc accggatgga tgccgaaacg
      781 cacgttgacg cccttcttga tcagggtctt ggcgatctcc gcgaccgcat gctgcgcctg
      841 cgcggtggcc atgccgtaac cggggaccac catgaccttg ttggcgttgt tcagcatctg
      901 cgcgacctct tcgaccgtgg tggtctgcac ctcgccctcg tagccggcgc tgacaccgcc
      961 gctggtggtg ccgaagccgc cggcgatgac actgataaaa tggcggttca tcgcgcggca
     1021 catgatatag ctgaggatcg cgccgctgct gccgaccagc gcgccggtga cgatcagcag
     1081 gtcgttggaa agcatgaagc cggtggccgc ggccgcccag cccgagtaac tgttgagcat
     1141 cgaaatcacc accggcatgt cggcaccgcc gatggcggcc accatgtgaa tgccgaacag
     1201 caacgccagc acggtcatcg ccagcaacag cagggtcgcc acatgatgat cctcggcacc
     1261 gacgaatttc gcgccaatca ctagcacggc aataaatatc gccaggttca gccagtggcg
     1321 ccctggcagc atcatcggcg cgccgccgat caggccgctg agcttgccgt aggcgatcac
     1381 cgagccggta aaggtcagcg cgccgatggc aatgccgatg tagatttcga tttcgtggat
     1441 ggttttctcg acgccgatat aggatatcga ggtatccatg aaattggcga aaccgatcag
     1501 taccgcggca agcccgacca ggctgtgcag gatcgcgacc agttccggca tctgcgtcat
     1561 cttgacgcgg cgcgcgagaa tgaagccgat gatgccgccg gcggccatca gccccaccag
     1621 cacctcgtaa tgccggttga cgaaggcgac gatggtgaca gcgaccgcga tcgccatgcc
     1681 gatcatgccg aacaggttgc cgcgacgcgc ggtttcgttg ttcgacaggc cgccgagcgc
     1741 gaggatgaac agcgccgcgg cgccgagata ggatacggtt accaggctgg aagtcattac
     1801 gcgtctcccc tgaacatgcg cagcatgcgc tgcgtcaccg cgaaaccacc gacgatatta
     1861 atgcaggtga tgaacactgc gatcgccgcg agcaggtaga tcagcccgct ggggctcgat
     1921 atctgcgtca acgcgccgag gatgatgatg ctgctgatcg cgttggtcac gctcatcaac
     1981 ggcgtgtgca gtgacggagt cacgttccag atcaccatat aaccgatgaa acaggaaagt
     2041 acgaagaccg tgaaatggct catgaacgac ggcggcgcga cctggccgag gccgagcaaa
     2101 gccaggccgg cggcgataac cgggaaccag gcgctcttga aaaagtcggc gacagccgat
     2161 gactggatca ccgcatcagg cgccttttcc tgttcctccg gcttgatttc ctgtgcgccg
     2221 taactcggct tgcgttcggc gctgaccgcg atcggcggcg gcggccaggt gatctctccg
     2281 tccttgacca cggtagcgcc gcgcagtacg tcgtcttcca t
//
LOCUS       DATCZV010000116         4269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_643487, whole genome shotgun sequence.
ACCESSION   DATCZV010000116 DATCZV010000000
VERSION     DATCZV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643487"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VIW27_02610"
     CDS             <1..456
                     /locus_tag="VIW27_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5738585.1"
                     /translation="VSRHGRPHADVDEMHAMDALDAVLPRMDYVFVSLPATPETVGLF
                     DRERLQLLKPGAGIVNVGRGATFDYDALAELLAEGRLSGAIIDVFDQEPLPADSPLWT
                     APNFIVTPHVSADDGDSYVPITLQLFFDNLRRYLAGDALRNVVRPELGY"
     gene            460..978
                     /locus_tag="VIW27_02615"
     CDS             460..978
                     /locus_tag="VIW27_02615"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY5738586.1"
                     /translation="MGVIDDLVDWLQSFDERISLRELQRRLDDDRLTPASLADYIHFS
                     DQHYARNLLAHGSQFYALVLCWKPGQASPIHDHKGASCGVRVIQGTATETSYHWQQGQ
                     LVPARVTEMQAGEVCGSFDDDIHEIRNNGDQDLVTLHVYSPYLDNINLYEIDSGKVTV
                     FSDPLIAQLKSG"
     gene            1067..2581
                     /locus_tag="VIW27_02620"
     CDS             1067..2581
                     /locus_tag="VIW27_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY5738587.1"
                     /translation="MSASKTIAVGGFQHETNTFAPHLASFEAFARADSWPALSSGEAL
                     FETMAGLNIPLSGFIDRARQHGHQLRPLLWCSAEPSSYVTRDAFERIAAMICDGLRDS
                     APLDGVYLDLHGAMVTEHYEDGEGELLRRVRQVVGTEVPVVISLDLHANVTAAMVEYS
                     DTMTIYRTYPHLDMATTGARACEQLQFLLSGQTLHKAMRKIPFLVPLTSQCTDFEPCR
                     SLYRSLQTTGGAGGISSVDFAAGFPPADIAECGPAVVAYGTDRDAVEAAAGEVFRRVV
                     DAESEFEFELLPADEAVARALSNESDKPVVLADAQDNPGAGGTSDTTGLLESLVRGGA
                     RGAVLAVLYDPAVAALAHAAGIGALLETELGAKSGFAGVEPYRGRFQVEALGDGRFVY
                     TGAMHLNVHAELGDMALLRIVDAHSEVRVVVGSKRAQCLDLAIIRHLGIEPTAQKILA
                     VKSTVHFRADFDPVAAATLVAIAPGANYCRLAEMKYCNLRPGVRLQPQGPVHRG"
     gene            complement(2694..>4269)
                     /locus_tag="VIW27_02625"
     CDS             complement(2694..>4269)
                     /locus_tag="VIW27_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738588.1"
                     /translation="SVGSSWVAGDAYRRARRYQEAIKIFRRMPHPPPSISAYIASCFA
                     GMGLVDEGRAEMKRFLKLARQQMPNFPDSIASWREYWRNSTAFRHDEDFELFFSQLLN
                     TGLCDELDGDKPELPSILVLPFSNQSGDPEQEYFSDGITDSIIMNLAASPGLQVKSRH
                     ASFAFKDSARAVDEIAAELGVRYIVAGSIRKAGDKVRISAQLSETGSGNQIWGKRFDS
                     DLANLFALEEDLSMTIAGSISTRIGKQARLSALHKPAKDLQSYDYYMRGSYYLELFTA
                     ENIGIAMELFDKCLSLDPDNSLAHAKLGIAHMVSLYENCSLDRERSLKLMDQHLRRAL
                     ELDPDDAEAHAFMAEMLMYRRDFDRALVHARKAVELNPTLADGYSMLAWHDGAVGKLD
                     EARAWAEKSMQTDIHHPYAGWNAGEIYRLCGDYELAIETFRSMAHMSTSVQAQIAACL
                     AGLNRLDEARAEMRRYLELAREQMSAMPSSREEWYDFWWESMPYKQQVDNDRYFELLL
                     QAGLCDQSENSAERKP"
BASE COUNT          751 a         1346 c         1332 g          840 t
ORIGIN      
        1 gtcagccgcc acggccggcc gcatgccgat gtcgacgaga tgcacgcgat ggatgcgctc
       61 gacgcggtgc tgccgcgcat ggattatgtt ttcgtctcac tgccggcgac gccggaaacc
      121 gtcggcctgt tcgatcgcga gcgcctgcag ttactcaagc ccggcgccgg catcgtcaac
      181 gtcggccgcg gcgcgacctt tgactacgac gcgctcgccg aactgcttgc cgagggccgc
      241 ctgtcgggag cgatcatcga cgttttcgac caggaaccgc tgccggcgga ctcgccgctg
      301 tggacggcgc ccaacttcat cgtcaccccg cacgtgtccg ccgacgacgg cgacagctac
      361 gtgccgatca cgctgcagct gtttttcgac aacctgcggc gctacctggc cggcgatgcg
      421 ttgcgcaacg tggtgcggcc ggagctggga tactgactca tgggcgttat cgacgacctt
      481 gtcgactggc tgcaatcctt cgatgagcga atttcactgc gcgagctgca acggcggctt
      541 gacgacgacc gcctgacgcc ggcgagcctc gccgactaca tccatttttc cgaccagcac
      601 tacgcgcgta acctgctggc ccacgggtcg caattctacg cgctggtgct gtgctggaaa
      661 ccggggcagg cgagcccgat tcacgaccac aagggcgctt cctgcggtgt ccgcgtgatc
      721 cagggcaccg cgaccgagac cagctatcac tggcagcagg ggcaactggt gccggcgcgc
      781 gtgaccgaga tgcaggccgg cgaggtctgc ggctccttcg acgacgatat tcacgaaatt
      841 cgcaataacg gcgatcagga cctggtcacg ttgcacgttt actcgcccta tctcgacaat
      901 atcaatctct atgaaatcga cagcggcaag gtcacggtat tttccgatcc actgatcgca
      961 cagctgaaat ccggctgacg actgccgccc gccgcgacaa tcgccagggc tttcaattcg
     1021 atgccgaatg cataccctgt aagcgattgc gcccacagga tttatgatgt cagcttcgaa
     1081 aaccatcgcc gtcggcggct ttcagcacga aaccaatacc ttcgcgccgc acctggcgtc
     1141 gttcgaggca tttgcgcgcg ccgacagctg gccggcgctc agcagcggcg aagccctgtt
     1201 cgagaccatg gccggactca atatacccct gtcgggattc attgatcgcg cgcgccagca
     1261 cggccaccag ctgcggccgc tgctgtggtg ttccgccgag cccagcagct atgtcacccg
     1321 cgatgccttc gagcgcatcg cggcgatgat ttgcgatggc ttgcgcgaca gtgcgccgct
     1381 cgacggcgtt tatctcgacc tgcatggcgc catggtgacc gagcattacg aagacggtga
     1441 gggtgaattg ctgcgccgcg ttcggcaagt cgtcggtact gaagtgccgg tcgtgatcag
     1501 cctcgacctg cacgccaacg tcaccgccgc gatggtcgaa tacagcgaca cgatgaccat
     1561 ctaccgcacc tatccgcacc tcgacatggc gaccaccgga gcgcgcgcct gcgagcagct
     1621 gcaatttttg ctgtcggggc agacgctcca caaggcgatg cgcaagattc cattcctggt
     1681 gccgctgact tcgcaatgca ccgacttcga gccctgccgg tcgctgtacc ggtcgctgca
     1741 gactacgggc ggcgcgggcg gtatttcgag tgtcgatttc gcggccgggt tcccgccggc
     1801 ggacatcgcc gagtgcgggc ccgcggtggt cgcctacggc accgaccgcg atgcggtcga
     1861 ggccgccgcc ggtgaggtat ttcgccgtgt cgtcgatgcc gagtccgagt tcgaattcga
     1921 gctgctgccg gccgacgagg cggtggcacg cgcgctaagc aacgagtcgg acaagcccgt
     1981 ggtgctggcc gacgcgcagg ataatcccgg tgccggcggt acctcggata ccaccggttt
     2041 gctcgaatcc ctggtacgcg gcggtgcgcg cggcgcggtg ctggcggtgc tctacgatcc
     2101 ggcggtcgcg gcactggcgc acgcggcggg tatcggcgcg ctgctggaga cggagctggg
     2161 cgccaagtcc ggttttgctg gcgtcgagcc ttatcgtggc cgtttccagg tcgaggcgct
     2221 cggcgacggc cgcttcgtgt ataccggcgc gatgcatctg aatgtccacg cggaactcgg
     2281 cgacatggca ttgctgcgca tcgtcgacgc gcattccgag gtgcgcgtgg tcgtcggcag
     2341 caagcgcgcg caatgcctcg atctcgcgat catccgtcac ctcggcatcg aacccaccgc
     2401 gcagaagata ctggccgtca agagcacggt gcatttccgc gccgatttcg atcccgtggc
     2461 ggccgcaacc ctggtcgcga tcgcgcccgg ggccaactat tgccgacttg ccgagatgaa
     2521 atactgtaac ctgcgtccag gtgtgcggct ccaaccgcaa gggcccgtcc accgcggctg
     2581 aaccaggtcg aaattctcca gcttggcgct aatgctggtg cagagtaaac aaacggttac
     2641 atggcgagtc ggctcgatac ttgccttccg cggtgtcgcc ttcggcaggc agctcaaggt
     2701 tttcgctccg cggaattttc cgattgatcg caaagaccgg cctgcaacaa caattcgaaa
     2761 tatcgatcgt tatcgacctg ttgcttgtag ggcatcgatt cccaccagaa gtcataccat
     2821 tcctcgcggg aagacggcat cgccgacatt tgttcgcgcg ccagctcgag gtagcggcgc
     2881 atttcggcgc gcgcctcgtc gaggcgattc aagccggcca ggcaagccgc gatctgcgcc
     2941 tgcacgctgg tcgacatgtg cgccatcgaa cgaaaggttt cgatcgccag ttcgtagtcg
     3001 ccgcagaggc gataaatttc accggcattc cagcccgcgt agggatggtg gatatcggtt
     3061 tgcatgcttt tctcggccca ggcccgggcc tcgtcgagct tgcccaccgc gccgtcatgc
     3121 caggccagca tcgagtaacc gtcggcgagc gtcgggttca gttcgaccgc cttgcgcgca
     3181 tgtaccagcg cgcggtcgaa atctcttctg tacatgagca tctcagccat gaaggcatgc
     3241 gcctcggcgt cgtcggggtc gagctccagc gcgcggcgca gatgctggtc catcagcttt
     3301 aaggagcgct cccggtcgag gctgcagttc tcgtagagtg acaccatgtg ggcgatgccc
     3361 agcttggcgt gggccaggga attgtccggg tcgagcgaca ggcatttgtc gaagagctcc
     3421 atggcgatgc cgatattttc ggcagtaaac agttccaggt agtaactgcc gcgcatgtaa
     3481 tagtcgtagc tttgcaggtc tttcgccggt ttgtgtaacg cgctgagacg cgcctgcttg
     3541 ccgatacggg tgctgatcga gccggcgatt gtcatgctca gatcttcctc gagcgcaaac
     3601 aggttggcga ggtcggagtc gaagcgtttg ccccagatct ggttgccgct gccggtctcc
     3661 gataactgcg cgctgatgcg aaccttgtcg ccggccttgc gaatgctgcc cgcgacgatg
     3721 tagcgaacac ccagttcggc cgcgatctcg tcgacggcgc gcgcgctgtc cttgaacgca
     3781 aaactggcat ggcgggactt gacctgcagg ccgggagacg cggccaggtt catgatgatg
     3841 ctatcggtga tgccgtcgct gaaatattcc tgctccggat cgccggactg gttgctgaat
     3901 ggcagcacca ggatcgacgg cagttccggt ttatcgccgt cgagttcgtc gcacaggccg
     3961 gtattcagca gttgcgaaaa aaataactcg aaatcttcgt cgtggcgaaa ggccgtggaa
     4021 tttcgccagt attcgcgcca ggaagcgatg ctgtccggga aattcggcat ttgctgtctc
     4081 gcgagcttca ggaagcgctt catttctgcc cgcccttcgt cgaccagacc catccccgcg
     4141 aaacagctcg cgatgtaggc gcttatcgag ggcggtggat ggggcatgcg gcggaatatt
     4201 tttatcgcct cctggtagcg cctcgctctt cggtaagcgt cgccagctac ccagctcgag
     4261 ccaacactg
//
LOCUS       DATCZV010000117         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_484614, whole genome shotgun sequence.
ACCESSION   DATCZV010000117 DATCZV010000000
VERSION     DATCZV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484614"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VIW27_02630"
     CDS             complement(<1..736)
                     /locus_tag="VIW27_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY5738589.1"
                     /translation="MSAADVLNKINESGAKFVDFRFTDTKGKEQHVSVPASMVGDDTF
                     TDGKMFDGSSVAGWKGINESDMILMPDPDTAVLDPFTDEPTVNISCNVVEPSTGEGYE
                     RDPRSIALRAEAYLKSTGIADTAYFGPENEFFVFDDVKWSTEMGHCSYQIESEEAAWS
                     SIKLYEDGNLGHRPGIKGGYFPVPPVDSLHDIRSAMCLAMEEMGLKVEVHHHEVATAG
                     QCEIGVAFNTLVRKADEVQVLKYRSEE"
     gene            complement(871..1980)
                     /locus_tag="VIW27_02635"
     CDS             complement(871..1980)
                     /locus_tag="VIW27_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5738590.1"
                     /translation="MTQRVTLVGAAVNCLLATLQIVFGVLGKSQALFADGLHTLSDLS
                     TDFIVLYASRRASREADKGHPYGHGRIETLASMLLGGILFAVGVGIGLRGLQSIASPS
                     GIDPEWITVVFAGLAIVAKEGLYRYTLKAAREHHSSLLESNAWHHRSDALSSLVVVAG
                     ISAQLLGVPYMDAAAAIVVAVMIMLMGYRLSRKALNELIDAGLDFELVDEVREAMLAD
                     TSVVGIHNLRSRSMGGQGYIDAHIEVDSNLTVSEAHYIAHKIEHRVKSQFPRIVDVQI
                     HIDPLDDGIKESVLARLPERSTIELDLQNAWRDIDAGARVSQIKLHYLDRLIEIDLVL
                     PASLSGHEHRPEIDALRESASSLDYIGKVNIYYQN"
     gene            complement(1994..2233)
                     /locus_tag="VIW27_02640"
     CDS             complement(1994..2233)
                     /locus_tag="VIW27_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY5738591.1"
                     /translation="MTADVEIDARRLFCPLPVIKLQDCIRRQVAGTRVRIVSTDPGSL
                     NDIPTWCRISGHRVLDIDDRETEFVFLVEKGANEE"
     gene            complement(2230..3246)
                     /locus_tag="VIW27_02645"
     CDS             complement(2230..3246)
                     /locus_tag="VIW27_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HEY5738592.1"
                     /translation="MSGGLDSLLAARLIQQQGIHVEGINFYTGFCVEGHTHAIRNRDR
                     RRVKRNNALWVAEQLGIKLHIIDIVDEYKDVVINPRHGYGANLNPCLDCKIFMVGKAR
                     EWIDENGFDFIITGEVIGQRPKSQRKRFMPLISTESGADDRLLRPLCAQNLAPTLPEH
                     AGWVDRELLKDFSGRSRKPQMELAEEFGFRDYAQPAGGCCFLTDQTYSVKLRDLWDNR
                     GRREYELDDIMLLKVGRHIRPSQSYKLIISREEGETNFLSGYRHLFPSIKTVSHPGPL
                     TLIDGGPLDQQQIELACAITARFSKGRDANSVELCYNDRRQEHSIHVKPLPAEQLRQD
                     WVVG"
BASE COUNT          600 a          953 c         1019 g          675 t
ORIGIN      
        1 gctcttccga tctgtacttc aaaacctgga cttcgtcggc cttgcgcacc agcgtgttga
       61 aggcgacgcc gatctcgcac tggccggcgg tcgctacttc gtggtgatgg acctcgacct
      121 tgaggcccat ttcttccatc gccaggcaca tcgcggagcg aatgtcgtgc agcgaatcga
      181 ccggtggaac cgggaagtaa ccgcccttga tgcccggacg atgaccgagg ttgccgtcct
      241 cgtagagctt gatcgagctc cacgcggctt cctcggactc gatctggtag gagcagtgac
      301 ccatctcggt ggaccacttg acgtcgtcga acacgaagaa ttcgttttcc gggccgaagt
      361 aggcggtgtc cgcgatgccg gtagacttca gataggcttc cgcgcgcagc gcgatcgagc
      421 gcgggtcgcg ttcgtaacct tcgccggtcg acggctcgac gacgttgcag ctgatgttga
      481 cggtcggttc atcggtgaac gggtcgagca ccgcagtgtc cgggtcgggc atcaggatca
      541 tgtccgactc gttgatgcct ttccagccgg ctaccgagga gccgtcgaac atcttgccgt
      601 cggtaaaggt gtcgtcgccg accatgctgg ccgggaccga aacgtgttgc tccttaccct
      661 tggtatcggt gaagcggaaa tcgacgaact tggcgccgga ctcgttaatc ttgttcagca
      721 catcagcagc ggacatggtt gtatctccag aatcgggggt caaaaaacag ggtgagaatt
      781 gtaacagtta acggcgcgat gccaatttat cgtgcaaatt cgcggggaaa ggtcagtctt
      841 tggtattaaa tgcactatta tagtgcaaaa ttagttttga tagtagatgt tgaccttgcc
      901 gatgtagtcg agactactgg cgctttcgcg cagcgcatcg atctccggcc ggtgttcgtg
      961 gccactcagc gaagccggca gcaccaggtc gatctcgatt agccggtcca ggtagtgcag
     1021 cttgatctgg ctgacccggg cgcctgcgtc gatatcgcgc caggcattct gcaggtcgag
     1081 ctcgatcgtg ctgcgctcgg gcagccgcgc gagcaccgat tccttgatgc cgtcgtcgag
     1141 cggatcgata tggatttgca cgtcgacgat tcgcggaaac tgtgatttca cccggtgctc
     1201 gatcttgtgc gcaatgtaat gcgcctcgga aaccgtcagg ttggaatcga cctcgatatg
     1261 cgcatcgata tagccctggc cgcccatcga gcgtgacctg aggttatgaa tgccgaccac
     1321 gctggtgtcg gccagcatcg cctcgcgcac ttcgtcgacc agttcgaaat cgagcccggc
     1381 gtcgatcagt tcgttgagtg ccttgcggct caggcgatag cccatcagca tgatcatcac
     1441 cgcgaccacg atcgccgccg ccgcgtccat gtacggcacg cccagcagct gggccgagat
     1501 gccggcaacc accaccagcg acgacaacgc atccgaacga tggtgccagg cattggattc
     1561 gagcaggctg gagtgatgtt cgcgtgcggc cttcagcgtg tagcggtaaa ggccttcctt
     1621 ggcgacgatg gcgagaccgg cgaacaccac ggttatccat tcgggatcga tgccggatgg
     1681 cgatgcgatg ctctgcaaac cgcgcaggcc gataccgacg ccgaccgcga acaggatgcc
     1741 gccgagcagc atcgacgcca gggtttcgat gcggccgtga ccgtaggggt gccccttgtc
     1801 ggcttctcgc gacgcccgcc gtgacgcata gaggacgatg aaatcggtac tcaggtcgga
     1861 cagcgtatgc aggccgtcgg cgaatagcgc ctgggatttg ccgagcacgc cgaagacgat
     1921 ctgcaacgtc gccagcaggc agttcactgc cgccccgacc agggtcacgc gctgggtcac
     1981 gcgagagcgt gtttcattcc tcattcgcgc ctttttcaac caggaagacg aattcggttt
     2041 cacggtcgtc gatgtccagt acccggtggc cgctgattcg gcaccaggtt ggtatatcgt
     2101 tgagactgcc ggggtcggtg ctgacgatac gcacgcgcgt cccggccacc tgtcgtcgaa
     2161 tacagtcctg cagcttgatc acgggcaatg ggcagaacag tcggcgggcg tcgatttcga
     2221 catcggcggt catccgacga cccagtcttg ccgtagctgc tcggccggca gcggcttgac
     2281 gtgaatgctg tgctcctgcc ggcgatcgtt gtagcacaat tccacgctgt tggcatcgcg
     2341 gcccttgctg aagcgcgcgg tgatcgcgca ggccagttca atttgctgct ggtcgagcgg
     2401 gccgccgtcg atcagcgtca gcggaccggg atggctgacg gtcttgatgc tcgggaacag
     2461 gtgccggtag ccggacagga aattggtctc gccttcttcg cgggagatga tcagcttgta
     2521 ggactggctg gggcgaatgt gtcggccgac tttcagcagc atgatatcgt cgagttcgta
     2581 ttcgcgccgc ccgcgattgt cccacaggtc cctgagcttg accgagtaag tctggtcggt
     2641 caggaagcag cagccgcccg ccggctgggc gtaatcgcga aatccgaact cctcggcgag
     2701 ctccatctgc ggcttgcgcg agcggccgct gaagtctttc agcagttcgc ggtccaccca
     2761 gccggcgtgt tccggcaggg tcggcgccag gttttgtgca cacagcggtc gcagcagacg
     2821 gtcatcggcg ccggactccg tggatatcaa cggcatgaag cgcttgcgtt gcgacttggg
     2881 tcgctggccg atgacttcac cggtgatgat gaagtcgaag ccgttttcgt cgatccactc
     2941 gcgagccttg ccgaccatga agatcttgca atccaggcag gggttcaggt tggcgccgta
     3001 gccgtgccgt ggattgatga cgacgtcctt gtactcgtcg acgatatcga tgatatgcag
     3061 cttgatgccg agttgctcgg cgacccacag cgcgttattg cgcttgaccc gccggcggtc
     3121 gcgattgcgg atcgcatggg tgtgcccctc gacgcaaaag ccggtataaa agttgatacc
     3181 ctcgacatgg attccctgct gctgaatcag ccgcgccgcc agcaacgaat cgaggccgcc
     3241 ggagatc
//
LOCUS       DATCZV010000118         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_590744, whole genome shotgun sequence.
ACCESSION   DATCZV010000118 DATCZV010000000
VERSION     DATCZV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590744"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VIW27_02650"
     CDS             complement(<1..458)
                     /locus_tag="VIW27_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY5738593.1"
                     /translation="MTDAALDKLVQGYRRYRDGEYQDSRPLIEDLVTRGQHPQIAVVA
                     CSDSRVDPAILFQADPGDLFMVRNVANLVPPMEEGGTYHGTSAALEFAVLGLGVEHIV
                     VLGHAHCGGIRLMMQPDPYDSAFKFVSAWVSMLTAAHRRVVATMAQADEAA"
     gene            complement(489..1235)
                     /locus_tag="VIW27_02655"
     CDS             complement(489..1235)
                     /locus_tag="VIW27_02655"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5738594.1"
                     /translation="MGALDGKTAVVTGGGQGIGLGISEALAAAGAEILVAQRSRPPAE
                     LGAHWVEADLSGTDSPAAIADAVERHWGRLDILVNNAGFMFETSLSALALDDWNRMLA
                     VNLTAPVFVTQALLPKLRDSGGSIVNIGSIEGLAANPGHIAYCATKGGVHALTRAMAV
                     DLGAQGIRVNAIAPGWIRSELSDNYINAQADPDAAWEDLHRMHPVGRVGEPADVGQLA
                     VYLAGDHAAFVTGQVIVIDGGRTSKLPLPF"
     gene            complement(1235..2002)
                     /locus_tag="VIW27_02660"
     CDS             complement(1235..2002)
                     /locus_tag="VIW27_02660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5738595.1"
                     /translation="MRFDDKSVVITGGVGGIGQALVELFAAEGARVLFSDRSEPDCSA
                     YTRKLADSGFSTRFLAGDLREKAFCEALIDEAVTVHGGLDILINNAGIIPRGNILETT
                     DEMWFSALDVNLTAVFYLCRAAIPHLRAAGGGAIVNTSSTWGIYPGPGHVAYCTSKAA
                     VAALTRNLGRDHAADGIRVNAVCPHEVNTPMLRSGFERRGIDPRQGIEELGKTVPIGH
                     VAEPREIADVIAFLASHDARYVCGETVEVSGAKPVSG"
BASE COUNT          378 a          735 c          766 g          406 t
ORIGIN      
        1 gcggcctcgt cggcctgtgc catggtcgcc accacgcggc ggtgcgcggc ggtcagcatc
       61 gatacccagg cggacacgaa cttgaaggcg ctgtcgtagg ggtcgggttg catcatcagc
      121 ctgatgccgc cgcagtgcgc atgcccgagc accacgatat gctcgacgcc gaggccgagg
      181 acggcaaact cgagcgccgc gctggtgccg tggtaggtac cgccttcttc catcggcggc
      241 accaggttgg cgacgttgcg caccatgaac aggtcgccgg ggtcggcctg gaacaggatc
      301 gccgggtcga cgcgcgagtc ggagcaggca acgaccgcga tctggggatg ctggccgcgg
      361 gtgaccaggt cctcgatcag cggacgactg tcctggtatt cgccgtcacg gtagcggcgg
      421 tagccctgta ccagcttatc gagcgcagca tcagtcataa acgttatcct gacaggtttc
      481 ggcgccaatt aaaatggcag cggcagtttg ctggtgcgac cgccatcgat gacgatcacc
      541 tggccggtga cgaaagcggc atggtcgccg gcgaggtaga cggccagctg gccgacgtcg
      601 gcgggctcgc cgacgcggcc gaccgggtgc atgcggtgca aatcttccca ggccgcgtcg
      661 ggatcggcct gcgcgttgat gtaattatcg ctgagctcac tgcgaatcca gcccggcgcg
      721 attgcgttga cacgaatccc ttgcgcgccg agatcgaccg ccatcgcccg cgtcagcgcg
      781 tgcacgccac ccttggtcgc gcagtaggcg atatgcccgg gattggccgc gaggccctcg
      841 atcgagccga tgttgacgat gctgccgccg ctgtcgcgca gcttcggcag cagcgcctgg
      901 gtgacgaata ccggcgcggt caggttgacc gcgagcatgc gattccagtc gtcgagtgcg
      961 agtgcggaca gcgaggtttc gaacatgaaa ccggcattgt tgaccaggat gtcgagcctg
     1021 ccccagtgcc gttccaccgc gtcggcgatc gccgccgggc tgtccgtccc ggacaggtcc
     1081 gcctcgaccc agtgcgcgcc gagttcggcc ggcggccggc tgcgttgcgc gaccaggatt
     1141 tcggcaccgg ccgcggcaag agcttcgctg ataccgagcc cgataccctg gccgccgccg
     1201 gtgactaccg cggtcttgcc gtcgagtgcg cccatcagcc gctcaccggc ttggcgccgc
     1261 tgacttcgac ggtttcgccg cacacatagc gcgcgtcgtg cgaggccagg aaggcgatca
     1321 cgtcggcgat ttcccgcggt tcggcgacat gcccgatcgg caccgtcttg ccgagttcct
     1381 cgattccctg ccgcggatcg atgccgcggc gttcgaagcc gctgcgcagc atcggcgtat
     1441 tgacctcgtg cgggcagacc gcgttgacgc gaatgccgtc cgcggcgtgg tcgcggccga
     1501 ggttgcgggt cagcgctgcc accgcggcct tgctggtgca ataggccacg tggccgggtc
     1561 cggggtagat gccccaggtc gacgaagtat tgacgatcgc gccgccgcca gccgcgcgca
     1621 ggtgcggaat cgccgcgcgg cacaggtaga acaccgcggt caggttcacg tccagcgcgc
     1681 tgaaccacat ctcgtcggtg gtctcgagga tgttgcctcg cggaatgatg ccggcgttgt
     1741 tgatcaggat atcgaggccg ccgtgaaccg tcacggcttc gtcgatcagc gcttcgcaaa
     1801 aggccttttc gcgcaggtcg ccggccagga atcgcgtgct gaatccgctg tccgccagct
     1861 tgcgcgtgta cgccgagcaa tccggctcgc tgcgatcgga gaacagtacc ctggcgccct
     1921 cggcggcgaa cagttcgacc agcgcctggc cgatgccgcc gacgccgccg gtgatgacaa
     1981 cgctcttatc gtcgaatctc atcgctcgct tcccggccag tcggccgaca aattatggat
     2041 cgtcgcaata tatacgcatg atcgggatag cgaaaagcga cattaatcgg tagttgcgat
     2101 ctgctttttg tcatgcactt cgaatcaaag aaagcgggcc ggactgaagg cgcgaatatt
     2161 gtggctgggt tgctcgccgt tcgccagctc ggcgaccagc ttgccggtga taccggccag
     2221 cgtcagtccg aggtgctggt ggccgaacgc gtagatgatg tattccgaca ccgtgctgta
     2281 accga
//
LOCUS       DATCZV010000119         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_326220, whole genome shotgun sequence.
ACCESSION   DATCZV010000119 DATCZV010000000
VERSION     DATCZV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326220"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1092
                     /locus_tag="VIW27_02665"
     CDS             367..1092
                     /locus_tag="VIW27_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY5738596.1"
                     /translation="MKVVDNSAPPGSASRSSRGRVGVTTGNVAAQLRRRILDGDYAFE
                     ERLPAERSLAEEFGVARGTIRSVLQILEEQHLVTRQVGSGTYVTHREITNQQEIASVT
                     SPLEMNEVRIAIEPQMVRLAIANASSRELEELRDALQQCEDCGGDPEKFALGDAAFHM
                     ALAHCSRNKLMYWLYERISEVRRHAQWRTMKAKLLTPDRIAFYNGQHRRIYEAIAARD
                     TPSAVKLIKQHLYGVHDDLLETE"
     gene            1096..>2142
                     /locus_tag="VIW27_02670"
     CDS             1096..>2142
                     /locus_tag="VIW27_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEY5738597.1"
                     /translation="MTAPLFVSPPRVRSPGLSPLPTGTERYLVRAGGSVALTLAAGEQ
                     IELVNPEGLQAGEISAFDDDGHSDSGLLGTRSDGHGEALRAILEAGGSGADRLAALLA
                     QRNIDPAEVDSKRLFGAGSPAGDSIRFTAQQDLDCIIAVPGGRMRADRQDTATDLVAF
                     VHRQPARAEQPIVRLPEPLAEPLLDQRIAARTAFAYEVKAGQYIQIIDVEGRECSDFQ
                     CFDTPLLERGKERCLDATTTRHVVGASYPAPGLYSKFYDQNFEAMVEVVQDTCGRHDA
                     FGTACTAKYYDDMGYPGHVNCSDNFNQALQPFGVEPRKGWMAMNLFFNTNIDDSNQMY
                     FDEPWSRPGDYVLMR"
BASE COUNT          437 a          698 c          627 g          380 t
ORIGIN      
        1 aagacctcta ccccgatttc ctggacaatc tgatgccgaa tacgcacaaa tattggacca
       61 ctttcatttt cggtgttcat gcagctgact tgcgcgaccc gcggcggcct tcaaaaagcg
      121 ggctttctgc acaaatgaga cgcttaataa gtattttata tcagtaactt atatgcaact
      181 atcggaactg gatcatgacc tcgacagtac tcataccagg agcaaaagcg ttctggtgat
      241 ttcggtccaa tttcctgatt tcagcctctg tcagacttta taaccggctt gatttgtggt
      301 tttttatgac gcattattga accaatttac agtaccacgt tcctcacagg caaggagttt
      361 taccgcgtga aagtcgtcga taacagtgca ccgcccggca gcgcgtcacg gtcgagccgc
      421 ggccgtgtcg gcgtaaccac cggcaatgtc gccgcacagc tgcggcggcg aatcctcgac
      481 ggcgactacg ctttcgagga gcgcctgccc gccgagcgca gcctggccga ggaattcggc
      541 gtcgcccgcg gcaccatccg ctcggtgctg caaatcctcg aggaacagca cctggtaacc
      601 cgccaggtcg gcagcggcac ctatgtcacg caccgcgaaa tcaccaacca gcaggaaatc
      661 gccagcgtca ccagcccgct ggaaatgaac gaggtgcgca ttgccatcga gccgcaaatg
      721 gtgcgcctgg cgatcgccaa tgccagctcc cgcgagctcg aagagctgcg cgacgcgctg
      781 cagcaatgcg aggactgcgg cggcgacccg gaaaagttcg cgctcggcga cgccgctttt
      841 cacatggcgc tggcgcactg ctcgcgcaac aagctgatgt actggctcta cgagcgtatc
      901 agcgaagtac gccggcacgc acagtggcgg acgatgaagg ccaagctgct gaccccggat
      961 cgcatcgcgt tttacaatgg ccagcaccgg cgaatctacg aggcgatcgc cgctcgcgat
     1021 acgccgagcg cggtaaagct gatcaagcaa catctctacg gggtgcacga tgacttgctg
     1081 gagaccgaat aagtgatgac cgcgccgctg ttcgtgtcgc cgccgcgcgt gcgttcaccg
     1141 gggctcagcc cgctgcccac tggtaccgag cgctacctgg taagggccgg tggcagcgtc
     1201 gcgctgacgc tcgccgccgg cgagcagatc gaactcgtca atcccgaggg cctgcaggcc
     1261 ggtgaaatca gcgcctttga tgacgatggg cactctgaca gcggcctgct cggcacgcgc
     1321 agcgatggcc atggcgaagc cctgagggcg attctcgaag ccggcggcag cggtgccgac
     1381 aggctcgcag cgctgctcgc gcagcgcaac atcgaccccg ccgaggtcga cagcaagcgg
     1441 ctattcggtg ccggctcacc cgccggcgat tcgattcgtt tcaccgcgca acaggatctc
     1501 gactgcatca ttgccgtgcc cggcggccgc atgcgcgccg accgccagga tacggcgacc
     1561 gacctggtcg ccttcgtcca ccgccagccg gcgcgcgcgg aacagccaat cgttcgcctg
     1621 cccgagccgc tcgccgagcc gctgctcgac cagcgtatcg cggcgcgtac cgcctttgcc
     1681 tacgaagtca aggcgggcca gtacatccag atcatcgacg tcgagggccg cgaatgctcg
     1741 gacttccagt gcttcgacac accgctgctt gagcgcggca aggagcgctg cctggacgct
     1801 accacgaccc gccacgtggt cggcgcatcc tatccggcgc cgggactcta ctcgaagttc
     1861 tacgaccaga acttcgaggc catggtcgaa gtcgtgcagg atacctgcgg acgccacgac
     1921 gccttcggca ccgcctgcac cgcgaagtat tacgacgaca tgggctaccc agggcacgtc
     1981 aactgctcgg acaatttcaa ccaggcgctg cagccgttcg gagtagagcc tcgcaagggc
     2041 tggatggcga tgaacctgtt cttcaacacc aacatcgacg acagtaacca gatgtacttc
     2101 gacgagccct ggtcacgacc cggtgactac gtgttgatgc gc
//
LOCUS       DATCZV010000120         9047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_379264, whole genome shotgun sequence.
ACCESSION   DATCZV010000120 DATCZV010000000
VERSION     DATCZV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379264"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VIW27_02675"
     CDS             <1..700
                     /locus_tag="VIW27_02675"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEY5738598.1"
                     /translation="RLQVDSALSRQVYIDRAAIEAFVASALYPINFLDFETFNSAIPK
                     FPGQQPYRTAIPFQYSLHVLHADGELEHREFLADEHRDPRAEIARQLAADITTEGSIV
                     AYSQATEKSIIRRLAADSAEQAQTLLAMDARFIDLLQPFQQLMYYHPDFNGSFSIKSV
                     LPAMFPDDETLDYKGLDIQAGDMASMIYADLDRIDDASERRKIRQALLAYCKLDTLAM
                     VMIWRELSEIAERG"
     gene            complement(789..1295)
                     /locus_tag="VIW27_02680"
     CDS             complement(789..1295)
                     /locus_tag="VIW27_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY5738599.1"
                     /translation="MRGLVVAALLVLFSLPAGAQQAGSGLTRLVDAPPAPDFSLLDMD
                     GTVFRLSDHRGRVLIVNFWATWCPPCREEMPAMQRAWEQLREEGMLMIGINVGEDEDT
                     IFQFTADYPVDFPLLMDSDSAVTGRWPVRGLPTTYVVDPAGRLVYQAIGSREWDDPAL
                     LELVRALR"
     gene            complement(1298..1900)
                     /locus_tag="VIW27_02685"
     CDS             complement(1298..1900)
                     /locus_tag="VIW27_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738600.1"
                     /translation="MMPVLRVPGRVKPIPALLCAIPLLLGSSAATVERGVDAETGLRS
                     WEWREAGVVLRLVQRLPDQTRAFFQARGFSGAASDRIADACIFQTIFRNESARPLGYS
                     LDDWRVLHRGERTTLMTRERWDAGWQEGEASQAAQIALRWSLLPTRQHFEPGDYNWGM
                     TGYGLPPGSAFDLELVLRLGEQPMMRTIDGIVCAPDREAN"
     gene            complement(2199..3023)
                     /locus_tag="VIW27_02690"
     CDS             complement(2199..3023)
                     /locus_tag="VIW27_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5738601.1"
                     /translation="MDEFIRFERLYRRFDLSEPWLARVIYRRPKKFLDAVDDVSFSIE
                     RGKTYALVGESGSGKSTIARMAVGLLSPSSGRVLIDGHDLYDPHLSNADNRRIRQRLQ
                     MVFQSPFASLNPRWRVGSILAEPLRVFKLVDGERAETARVEELLDQVGLSASDARRFP
                     HEFSGGQRQRISIARALASKPDFIVCDEPTSALDVSVQAQVLNLLKRLQRDLGLTYLF
                     ITHDLAVVRVMAHRIGVLKSGKLVEEQSTADLIESPQSDYTRMLIRSAPRIHREAS"
     gene            complement(3016..3975)
                     /locus_tag="VIW27_02695"
     CDS             complement(3016..3975)
                     /locus_tag="VIW27_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5738602.1"
                     /translation="MSDPILRVKNLRVVFPHRHGELVPIDDISFDVRRGEILGFVGES
                     GAGKSMTGAAIIGLIDPPGYIEQGEIWLEDERIDKLSERQAMRIRGKRISMVFQDPLT
                     SLNPLRTIGDQLVETILTHLPMSEEKARRKAMAGLEEVGIDPGRFNAYPHEFSGGMRQ
                     RVVIALALSAEPELIIADEPTTALDVSVQAQVLDLLKKLCREHGASVILITHDMGVIA
                     ETTDRVAVLYAGRLAEIGTTREVINEPLHPYTRGLIASTPSVETGDIDTRLFQIEGAM
                     PRLDALPAGCAFNPRCQNAEQRCRGQRPDLMQGRVACWLYTDG"
     gene            complement(3972..4898)
                     /locus_tag="VIW27_02700"
     CDS             complement(3972..4898)
                     /locus_tag="VIW27_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018632587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738603.1"
                     /translation="MSGERGAWQRFLESDFFFDFKRSPVTIVSFTVVVTLILLAVLAD
                     VVAPTNPFDPASLNLMNGFTPPMEPNAFTGETFVFGTDDQGRDLLSAIIYGLRVSLFV
                     GLTAVILAMLLGVSLGLLAGYRGGWIDSVIMRVADIQMTFPSILIAVLIFGVARGLLP
                     PDLRDELAISVLIIAIGLSEWVPFARTVRGTTMVEKEKEYVQAARLIGLGGGQIMLRH
                     ILPNVLSPVLVIATITLALAIIAEATLSFLGVGAPPTEPSLGTLIRIGQDFLFSGEWW
                     ILLFPAVTLLALALSVNLLGDWLRDTLNPRLK"
     gene            complement(4895..5881)
                     /locus_tag="VIW27_02705"
     CDS             complement(4895..5881)
                     /locus_tag="VIW27_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738604.1"
                     /translation="MLFIIARRLLQAFVVLLLVALVAFTIFRFIGDPVESLLGQEATV
                     ADRQALIDRMGLNDPIPVQYLRFVGNALQGDFGISYRTAEPVSTLIAERLPATLELAF
                     VSALFAMVVGTLLGIYTAIYRDGFLAHAIMSSSLIGVSLPTFLIGILLIWLFAVELGW
                     LPSFGRGETVQIGFWSTGFLTASGLKSLILPAITLGLYQMTLIMRLVRAEMLEVLRQD
                     FIRFARARGLPERVVYFGHALKNTMVPVITVAGLQLGAIIAFALITETVFQWPGVGLM
                     FINAVFFVDIPVMSAYLLLVGTVFVFINLIVDLLYLAIDPRLRDGQLRRRAT"
     gene            complement(5934..7475)
                     /locus_tag="VIW27_02710"
     CDS             complement(5934..7475)
                     /locus_tag="VIW27_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5738605.1"
                     /translation="MLISSIASAQTLRWARSGDSLTLDPHAQNEGPTHTLAHQIYEPL
                     LHRDMAGQITPALATSWKALADNPNIWRFQLRQGVTFHDGAAFTADDVVYSFNRARKP
                     TSAMKELLTSVKEIRKVSDHVVDFETNGPNPLLINNLTNLFVMDSGWAEKNGASDPQD
                     VAAGETTFASQNTNGTGAFMLVSRAPDQKTVLKANPNYWGKGQLPLQVSEIVYTPIQS
                     AATRVAALLSGEVDFIQDVPVQDLRRVASQSGLKVVTAAQNRVIFFGMNVGDSDIESD
                     NVSGKNPFADIRVRKAMNMAINRGAIQKVVMRDQSSPTNVIMPPFVNGWTEQLDQVPA
                     NDVALAKQMMAEAGYGDGFSVTLNCPNDRYINDEAICQAAVGMLGRIGIKVNLDAKPK
                     AQHFPLIKNKTTDFYMLGWGVPTFDSHYIFNFLVHTTTENRGSWNGTRYSNPVLDKKI
                     VALESETDLARRNGIIADIWRQVQADQIYLPIHNQVLNWGMKNNIQFDVQPEDQPHFK
                     YLTFN"
     gene            8309..>9047
                     /locus_tag="VIW27_02715"
     CDS             8309..>9047
                     /locus_tag="VIW27_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5738606.1"
                     /translation="MATQDWKPGLGLGLSGGGFRASFFHLGVLARMAEMGMLRHVEVI
                     STVSGGSIVGAAYYLGLKSLLESKPNADITDDDYRDVVARVEYQFRLAVQQNLRMRTF
                     ADLVKNIRMMLPHYSRSDSIGELYDGLIYRNLIDVRDDDSPIMMTDLFIEPDGDKNFH
                     PANQSVDGKPLHAKVPILLLNATSLNSGHNWIFTARSMGEVPPRNAVFRDVDKKDRYR
                     RVNYDQIDKSVRRPNFPLGSAVAASAAV"
BASE COUNT         1804 a         2848 c         2779 g         1616 t
ORIGIN      
        1 gcgattgcag gtcgactcgg cgctatcgcg gcaggtatac atcgatcgcg cggcgatcga
       61 ggcattcgtg gccagcgcgc tttacccgat caacttcctg gatttcgaaa ccttcaacag
      121 cgcgattccg aagttccccg ggcaacagcc gtatcgtacc gcgatcccgt ttcagtattc
      181 gctgcacgtg ctgcacgccg acggcgaact cgagcaccgc gaattcctcg ccgacgaaca
      241 ccgcgacccg cgtgccgaaa tcgcacggca actggccgcg gatatcacca ccgagggctc
      301 catcgtggcc tatagccagg ccaccgaaaa atcgattatc cgccgcctcg cggctgactc
      361 cgccgagcag gcacaaacat tgctggcgat ggacgcgcga ttcatcgacc tgttgcagcc
      421 gtttcaacaa ttgatgtatt accacccgga tttcaacggc agcttttcga tcaaatcggt
      481 attgccggcg atgtttccgg atgacgaaac gctcgactac aagggattgg atatccaggc
      541 cggcgacatg gcctcgatga tttatgccga cctcgaccgc atcgacgacg caagcgaaag
      601 gcgaaaaatc cgccaggcgc tgctcgccta ctgcaagctg gacacgctgg cgatggtaat
      661 gatctggcga gagctcagcg agattgccga acggggttag ccgacgccat ggtcaccgca
      721 ttcgcgaacg cccgctgccc gctaatcgag aactccttgc ggcccggcgc gtgcgcgagc
      781 cgggcgcgtt acctgagcgc ccgcaccagc tccagcagcg ccgggtcgtc ccactcgcgg
      841 ctgccgatcg cctggtagac cagccggccg gcgggatcga cgacgtaggt ggtcggcagc
      901 ccgcgcaccg gccaccgccc ggtgaccgcg gagtcgctgt ccatcagcaa cgggaaatcg
      961 accgggtagt cggcagtgaa ctgaaaaatc gtatcctcgt cctcgccgac attgatgccg
     1021 atcatcagca tgccttcctc ccgcaactgc tcccaggcgc gctgcatcgc cggcatttcc
     1081 tcgcggcacg gcggacacca ggtcgcccag aaattgacga tcaggacacg gccgcggtgg
     1141 tccgacaggc ggaaaaccgt accgtccatg tcgagcaggc tgaaatccgg tgccggcggc
     1201 gcgtcgacaa gccgcgtcag gccgctgccg gcctgctggg cgcccgccgg cagcgaaaac
     1261 agcaccagca gggcggcgac aacaagcccg cgcatgctca gttcgcctcg cgatcgggcg
     1321 cgcaaacgat gccatcgatg gtgcgcatca tcggttgttc tccgaggcgc aaaaccagct
     1381 cgagatcgaa agccgaaccg ggcggcaggc cgtaaccggt catgccccag ttgtaatcgc
     1441 ccggctcgaa atgctggcgg gtgggcagca gcgaccagcg tagcgcgatc tgcgcggcct
     1501 gactggcctc gccttcctgc caaccggcat cccagcgctc gcgggtcatc agcgttgtgc
     1561 gctcaccgcg gtgcagcacg cgccagtcgt ccagcgagta gccgagcggc cgcgcgctct
     1621 cgttacgaaa gatggtctga aagatacagg catcggcgat acggtcggac gcggcaccgg
     1681 aaaacccccg cgcctggaaa aaggcccgcg tctggtccgg cagtctttgc accagccgca
     1741 gcaccacgcc cgcctcacgc cactcccagg agcgcaaacc agtctccgcg tcgacgccgc
     1801 gctcgaccgt cgccgccgag ctgccgagca gcagcgggat tgcgcacaac agcgccggta
     1861 tcggcttaac gcggccaggc acgcggagca ctggcatcat cgaatcgagt ctcccggctg
     1921 aaacttcaac gccatacctt aaccgccgcg cctgcgtata tccagccttg acagctcgcc
     1981 gcgctgcaca atctgcagcg aagcttcgcg taagcgttgc gaatgacaca cccggcggcg
     2041 agattctcag tcatcgattt caaacgcccg tatttgcgtg tcactcaggg ggtattagat
     2101 ataaagctca gggaccaggc actgcaattc acaggcagtc ttcgatttca atgaaagata
     2161 cgaggctcgg cccctggcta atgggccgca ggccccgctc aggacgcttc gcgatgaatc
     2221 cgtggcgccg aacggatcag catacgcgtg taatccgatt gcggtgactc gatcaggtcg
     2281 gcggtggact gctcctcgac cagcttgccg gatttcaaca cgccgatccg gtgcgccatc
     2341 acgcgcacca ccgcgagatc gtgggtaatg aacaaatagg tcaaaccgag atcccgctgc
     2401 agccgcttga gcaggttgag cacctgcgcc tgcaccgaca cgtcgagcgc cgaggtgggt
     2461 tcgtcgcaga cgatgaaatc gggtttcgac gccagcgccc gggcgatcga gatgcgctgc
     2521 cgctgcccgc cggaaaactc gtgcggaaag cggcgtgcat cggaggccga tagtccgacc
     2581 tggtcgagca attcctcgac gcgcgcggtt tcggcacgct cgccgtcgac cagtttgaaa
     2641 acccgcaatg gctcggccag gatgctgccc acgcgccagc gcggattcag cgacgcgaac
     2701 ggcgactgaa acaccatttg caggcgctgg cgaatgcggc ggttgtcggc attcgaaaga
     2761 tgcgggtcgt acaggtcatg accgtcgatc agcacgcggc cactgctggg gctgagcaat
     2821 cccaccgcca tgcgcgcgat ggtcgattta cccgaaccgc tttcgcccac cagcgcgtag
     2881 gttttgccgc gctcgatact gaaggataca tcgtcgaccg cgtcgaggaa cttcttcggc
     2941 cgccggtaaa tcacccgcgc gagccagggt tccgacaggt cgaaacgccg gtacagccgt
     3001 tcgaagcgaa taaactcatc catcggtata caaccagcag gcgacccggc cctgcatcag
     3061 atcgggccgt tggccacgac agcgctgctc ggcattctgg caacgcggat tgaaggcgca
     3121 accggcgggc agcgcatcga ggcgcggcat cgcgccctcg atctggaaca ggcgtgtatc
     3181 gatgtcgccg gtctccaccg acggggtcga cgcgatcagg ccgcgggtat aagggtgcag
     3241 cggttcgttg atcacttcgc gggtggtgcc gatctcggcg aggcggccag catagagcac
     3301 cgcgacgcgg tcggtggtct cggcgattac ccccatatcg tgagtaatca ggatcaccga
     3361 ggcgccgtgt tcgcggcaca actttttcag caagtcgagc acctgcgcct gcaccgacac
     3421 gtcgagcgcg gtggtgggtt cgtcggcgat gatcagttcg ggctccgccg acagcgccag
     3481 tgctatcacc acgcgctgcc gcatgccgcc ggaaaattcg tgcgggtagg cattgaagcg
     3541 acccggatcg atgccgactt cctcgagccc ggccatcgcc ttgcgcctgg ctttttcttc
     3601 gctcatcggc aggtgagtga ggatggtttc gacgagctgg tcgccgatcg tgcgcagcgg
     3661 gttgaggctg gtcagcgggt cctggaaaac catgctgatg cgcttgccgc gaatgcgcat
     3721 cgcctggcgt tcgctcaatt tgtcgatgcg ctcgtcctcg agccagattt cgccctgctc
     3781 gatgtacccg gggggatcga tcaggccgat gatcgcggcg ccggtcatcg acttgccggc
     3841 acccgattcg ccgacgaaac cgagtatctc gccgcgcctg acgtcgaagg agatatcatc
     3901 gatcggcacc agctcgccgt ggcggtgcgg aaacaccacg cgcaggtttt tgactcgcag
     3961 gatcgggtcg ctcacttgag cctcgggttc agcgtgtcgc gcagccagtc gccgagcagg
     4021 ttgaccgaca gcgccagcgc cagcagcgtg accgccggaa acaacaggat ccaccactca
     4081 ccggagaaca ggaaatcctg gccgatgcgg atcagcgtgc cgaggctcgg ctcggtcggc
     4141 ggtgcgccga cgccgagaaa cgacagggtg gcctcggcga tgatcgccag cgccagcgtg
     4201 atggtggcga tgaccagcac cggtgacagt acgttgggca ggatgtggcg cagcatgatt
     4261 tgtccgccgc cgagcccgat caggcgcgcg gcctgcacgt attccttttc cttttcgacc
     4321 atcgtggtgc cgcgcacggt acgcgcgaac ggcacccatt ccgacaggcc gatggcgatg
     4381 atcagcacgc tgatcgcgag ctcgtcgcgc aggtccggcg gcagcaggcc acgggcgacg
     4441 ccgaagatca gcaccgcgat caggatcgac gggaaagtca tctggatatc ggcgacgcgc
     4501 atgatcaccg aatcgatcca gccgccgcgg tagccggcca acagaccgag actgacgccg
     4561 agcagcatcg ccaggatcac cgcggtcaga ccgacgaaca gcgacacgcg caggccgtag
     4621 ataatcgccg acagcaggtc gcggccctgg tcgtcggtgc cgaagacgaa ggtctcaccg
     4681 gtgaaagcgt tgggttccat cggcggcgtg aagccgttca tcaggttcag cgaagccggg
     4741 tcgaacgggt tggtcggcgc cacgacatcg gccagcaccg ccagcaggat cagcgtgacg
     4801 acgacggtga aggaaacgat ggtgaccggc gagcgcttga agtcgaaaaa gaaatcgctt
     4861 tcgagaaagc gctgccaggc gccgcgttcg ccgctcatgt cgcccgccgt ctcaactggc
     4921 cgtcgcgcag ccgcggatcg atcgccaggt agagcaggtc gacaatcagg ttgatgaaaa
     4981 cgaacacggt gccgaccagc agcaggtagg ccgacatcac cgggatatcg acgaagaata
     5041 ccgcgttgat gaacatcagg ccgacaccag gccactggaa cacggtctcg gtgatcaacg
     5101 cgaaggcgat gatcgcgccc agctgcaggc ccgccacggt gatcaccggc accatggtgt
     5161 tcttcagcgc gtgaccgaaa tagacgacac gctcgggcag gccgcgcgcg cgcgcgaagc
     5221 gaatgaaatc ctggcgcaac acctcgagca tctcggcgcg caccaggcgc atgatcagcg
     5281 tcatctggta gagaccgagc gtaatcgccg gcaggatcag cgacttgagc ccggacgcgg
     5341 tcaggaagcc ggtgctccag aaaccgatct gcacggtctc accgcggccg aagctcggca
     5401 gccagccgag ttccaccgcg aacagccaga tcagcaatat accgattaaa aaggtcggca
     5461 gcgaaacgcc gatcagcgaa ctcgacatga tcgcgtgggc gaggaagccg tcgcggtaga
     5521 tcgccgtgta gataccgagc aaggtaccga ccaccatcgc gaacaatgcc gagacgaagg
     5581 cgagctccag tgtcgccggc aggcgttccg cgatcagcgt cgaaaccggt tcggcggtac
     5641 ggtacgagat gccgaaatcg ccctgcagcg cattaccgac gaaacgcagg tactgcaccg
     5701 ggatcgggtc gttgagcccc atgcgatcga tcagcgcctg gcgatcggcc accgtggcct
     5761 cctgccccag caggctttcc accggatcgc ctatgaaacg aaatatggtg aacgcaacca
     5821 gcgcgaccag cagcaggacg acaaacgcct gcaacaggcg gcgggcgatg atgaacaaca
     5881 tgggcgcgtg aaaaaagccc ggccgcgggt ctagcccgcg gccggcgatt tgcctagttg
     5941 aaggtcaggt atttgaagtg cggctggtct tccggctgca cgtcgaactg gatgttgttc
     6001 ttcattcccc agttcagcac ctggttgtgg atcggcaggt agatctggtc ggcctgcacc
     6061 tggcgccaaa tgtcggcaat gatgccgtta cgcctggcga gatcggtctc cgactcgagc
     6121 gcgacgatct tcttgtcgag taccgggttg ctgtagcgcg tgccgttcca ggaaccgcgg
     6181 ttctcggtgg tggtgtgcac caggaagtta aagatgtagt gcgagtcgaa ggtcggtacg
     6241 ccccagccga gcatgtagaa gtcggtagtc ttgttcttga tcagcggaaa gtgctgcgcc
     6301 tttggcttgg catcgaggtt caccttgatg ccgatgcggc ccagcatgcc gaccgcggcc
     6361 tggcagatcg cctcgtcgtt gatgtagcgg tcgttcgggc agttcagcgt aaccgagaaa
     6421 ccgtcgccgt aaccggcttc tgccatcatc tgcttcgcca gggcgacgtc gttcgccggt
     6481 acctggtcga gctgctcggt ccagccgttg acgaacggcg gcatgatcac gttggtcggc
     6541 gaggactgat cgcgcatcac gaccttctgg atcgcgccgc ggttgatcgc catgttcatc
     6601 gccttgcgca cgcgaatgtc ggcgaaagga ttcttgccgc tgacgttatc gctttcgata
     6661 tcgctgtcgc cgacgttcat gccgaagaag atcacacggt tctgtgccgc agtgacgacc
     6721 ttgaggccgg actggctggc gactcgacgc agatcctgca ccggcacgtc ctggataaaa
     6781 tcaacctcgc cggagagcag cgcggcgacg cgcgtcgcag ccgactggat cggcgtatag
     6841 acgatctcgc tgacctggag cgggagctgg cccttgcccc agtaattcgg gttggccttg
     6901 agtacggtct tctggtcagg cgcacggctg accagcatga aagcaccggt gccgttggtg
     6961 ttctgcgagg cgaaagtcgt ctcgcccgcg gcgacgtcct gcggatcgct ggcgccgttc
     7021 ttctcggccc agccgctgtc catgacgaac aggttggtca ggttgttgat cagcaacggg
     7081 ttcggcccgt tggtctcgaa gtcgacgacg tgatcgctga ccttgcggat ctccttgacg
     7141 ctggtgagca gctccttcat cgccgaggtc ggtttgcgag cccggttgaa gctgtagacg
     7201 acgtcgtcgg cggtgaacgc ggcgccgtcg tggaaggtca cgccctggcg caactggaag
     7261 cgccagatat tcggattatc ggcgagcgct ttccagctgg tcgccagcgc cggggtgatc
     7321 tggccggcca tgtcgcggtg cagcagcggt tcgtagatct ggtgcgcgag ggtatgcgtc
     7381 gggccttcgt tctgcgcgtg cggatcgagc gtcagcgaat cgccggaacg ggcccagcgc
     7441 agagtctgcg cggacgcgat gctggatata agcattcccg cagccaaggc cacggcaaac
     7501 ctggtagtca gggtactcat gtttacctcc gaggtggatt aattattgtc tgaagcgatt
     7561 agttgttttt agaaacctgc acattaaacg atgacccgcg ccaagtttca aataaattca
     7621 atctatttga caaatcgacc gactgtcgcc catccagccc tggccgactc tattcctggt
     7681 cgatacgcct cagggcccac ccgctacaac ctggtgcgcg tccttcaccc ccgatgtgcg
     7741 ttgtcccgcc cctagcgcgc ctcatctcat catccacgca cctcatctca gcatccacac
     7801 gcctcatctc agcatccaca cgcgtcatcc cggcatccat gcacgtcatc ccggcgcaag
     7861 ccgggatcaa gcgcttgccg gcagttcctc tcgcaaactc cgccgaaaac cacccgctac
     7921 cggagtcata aatatcggca ccaggtcccg gatttcatcc gcggtctggc tctggatgca
     7981 agctgcaccg gctgattcca atccttcagg caagccctta ccgcaacaaa cggcgggctc
     8041 aggggttaca tttcacagat ggttttcagc ctcgaggtaa gatgtaaagc ctgccctcgg
     8101 ttcgcagaac cgagcgtatt tcacctggtg gccggcgaac gaattgccaa gcaaatatcg
     8161 gtctgtggta atatcttccg caagggtgag ccggggtgcg taatccggat tgtaatcgct
     8221 cgactgcagt gtcaaagaaa aatccgtaag ggtatcaccg aaggcgtcca atagccttta
     8281 accaagtctg caaccctggg agacgaaaat ggcaactcaa gactggaaac ctggattggg
     8341 cctcggcctt tccggcggcg gctttcgtgc atcttttttt cacctcggcg ttttggcgcg
     8401 catggcggag atgggcatgc tgcgtcatgt cgaggtgatc tcgaccgttt cgggaggctc
     8461 tatcgtcggc gcggcctatt acctgggcct taaatcgctg ctcgaatcga agcccaatgc
     8521 cgatatcacc gatgacgatt accgtgacgt cgtcgccagg gtggagtatc aattccggct
     8581 ggccgtgcag caaaacctgc gcatgcgcac cttcgccgac ctggtcaaga acatccgcat
     8641 gatgctgccc cattattctc ggtccgattc gataggcgaa ctttacgacg ggctgatcta
     8701 ccgaaacctg atcgatgtcc gcgatgacga cagcccgatc atgatgaccg acctgtttat
     8761 cgagcccgat ggcgacaaga attttcatcc cgccaaccag tcggtggacg gcaagccact
     8821 gcatgccaag gtaccgatcc tgctgctcaa cgcgaccagc ctgaattcgg gccacaactg
     8881 gattttcacc gcgcgcagca tgggcgaggt gccgccgcgc aacgcagtct ttcgcgatgt
     8941 cgataagaag gatcgatacc ggcgggtgaa ctacgaccaa atcgacaaga gtgttcgccg
     9001 cccgaacttc ccgctgggca gcgcggtcgc cgcttcggcg gcggtgc
//
LOCUS       DATCZV010000121         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_8920, whole genome shotgun sequence.
ACCESSION   DATCZV010000121 DATCZV010000000
VERSION     DATCZV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8920"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..578
                     /locus_tag="VIW27_02720"
     CDS             27..578
                     /locus_tag="VIW27_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY5738607.1"
                     /translation="MRQDLLIEFRGLELQTTWGLFSPRAVDDGSRLLLDYLELDETDD
                     CLDLGCGYGVLGLCMARAAPLGHTLLVDKDFVAVEYAEGNRQRNRIDNASCLLSNGFQ
                     QIPAQSFDVIVSNIPAKVGKEMLYIYLFDALAHLKPGGSFYIVTITGLRQFFKRGFAE
                     VFGHYEKIKQGKTYTVARGVKQQ"
     gene            575..991
                     /locus_tag="VIW27_02725"
     CDS             575..991
                     /locus_tag="VIW27_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY5738608.1"
                     /translation="MMADTGLLPGRLIIAGGAILALLAVGAGAFAAHGLRDSLDAYRL
                     GIFETAARYQMYHALATFGVGILAAVTPLPRRWLNVAALAFGAGIVLFSGSLYALALG
                     APRWLGAITPLGGTLFMLGWLAVAVAALKARRGAHG"
     gene            complement(998..1474)
                     /locus_tag="VIW27_02730"
     CDS             complement(998..1474)
                     /locus_tag="VIW27_02730"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY5738609.1"
                     /translation="MIRGRLSNPASPIATSSTNPILILAASGLLAILSIALGFVSIAE
                     RKHLLQDGRKTDGIVVAIDVGIKGLKSVEAQFTRADGRRVVGRDIHRTQWFAANEPGD
                     AVELHYDPEYDGDAPPDILIDRGAWIWSNPALLIFAGVALLALGHFLARQPRRDGG"
     gene            complement(1517..>2049)
                     /locus_tag="VIW27_02735"
     CDS             complement(1517..>2049)
                     /locus_tag="VIW27_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5738610.1"
                     /translation="VSCFPPAQNTHAGGILDVSLAPAPVAPELAAEAIDAATRIARGL
                     DYCGVLAVEFFISRDRRVLVNEMAPRPHNSGHYTLDGCETSQFEQQVRMICDLPAGSC
                     RQHTPVAMLNLLGDIWPELGNPRWESVLEHEHAKLHLYGKLEARPGRKMGHVNCLGES
                     LDEARALLDRVRAELG"
BASE COUNT          335 a          678 c          668 g          368 t
ORIGIN      
        1 ataacctcga agaatacaac cgtaaaattc gccaggacct gctgatcgag tttcgcggcc
       61 tcgagctgca gaccacctgg gggctgttct cgccgcgcgc ggtcgacgac ggttcgcgcc
      121 tgctgctcga ctacctggaa ctcgacgaaa ccgacgactg tctcgacctc ggctgcggtt
      181 acggcgtact cgggctgtgc atggcgcgcg ccgcgccgct tggccacacg ctgctggtcg
      241 acaaggattt cgtcgccgtc gagtacgcgg aaggcaaccg gcagcgcaac cggatcgaca
      301 acgccagctg cctgctgagc aacggtttcc agcaaatccc cgcccagtct ttcgacgtca
      361 tcgtctccaa tattccggcc aaggtcggca aggaaatgct ctacatctac ctgttcgacg
      421 cgctcgctca cctgaagccg ggcggatcct tctatatcgt caccatcacc gggctcaggc
      481 agtttttcaa gcgcggcttt gccgaggttt tcggccacta cgaaaagatc aagcagggca
      541 agacctatac cgtggcgcgg ggcgtcaaac agcaatgatg gccgacaccg ggctcctgcc
      601 gggccggcta atcatcgccg gcggcgcgat cctggccctg ctcgcggtcg gcgccggcgc
      661 attcgccgcg catggcttgc gcgattcgct cgacgcctat cggctcggca tcttcgaaac
      721 cgcggcgcgc tatcagatgt accacgcgct ggcgacgttc ggcgtcggca tactcgccgc
      781 cgtgacaccg ttgccgcggc gctggctgaa cgtcgccgcg ctcgcgtttg gcgccggtat
      841 cgtgctgttc agcggctcgc tgtatgcgct cgccctcggc gcgccgcgct ggctgggtgc
      901 gatcaccccg ctcggcggca cactgttcat gctcggctgg ctggcggtcg ccgtcgcggc
      961 attgaaggcc cgccgcgggg cgcacggctg acgggactca accaccgtcg cggcgtggct
     1021 gccgcgcgag gaagtggccg agcgcgagca gcgcgacgcc ggcgaatatc agcaatgccg
     1081 ggttgctcca gatccaggca ccgcggtcga tgaggatgtc gggcggcgcg tcaccgtcgt
     1141 attccggatc gtagtgaagc tcgaccgcat cgccgggctc gttggccgca aaccattgcg
     1201 tcctgtggat gtcccggccg actacccggc ggccgtcggc gcgggtgaat tgcgcttcga
     1261 cgctcttaag gccttttatt ccgacatcga tcgccaccac gatgccatcg gtcttgcgcc
     1321 cgtcctgcag taaatgcttg cgctcggcaa tgctgacgaa accgagcgcg atcgacagga
     1381 tcgccagcag tccgctggcg gcaaggatca ggatcggatt ggtgctgctg gtggcgatgg
     1441 ggctggccgg gttgcttaat cgtcctcgaa ttatagtccg ccggcaagcg cggcggcggc
     1501 aatcgccgaa tcggcttcag ccgagctcgg cgcgcaccct gtcgagcaac gcccgagcct
     1561 cgtcgagcga ttcgccgagg cagttgacat gccccatctt gcggccggga cgcgcctcga
     1621 gcttgccgta gaggtgcagc ttggcgtgtt cgtgttcgag taccgactcc cagcggggat
     1681 taccgagttc gggccagata tcgcccagca ggttgagcat cgccaccggc gtgtgctggc
     1741 gacaggagcc cgccggcagg tcgcaaatca tccgtacctg ctgctcgaac tgcgaggtct
     1801 cgcagccgtc gagcgtgtaa tggccggaat tgtgcggccg cggcgccatt tcgttgacca
     1861 gcacccggcg gtcgcgggag ataaaaaatt ccaccgccag cacgccgcag tagtcgagcc
     1921 cgcgggcgat gcgggtggcc gcgtcgatag cctcagcggc aagttccggg gctaccggcg
     1981 ccggcgccag gctgacgtcg agaatcccgc cggcgtgggt attttgcgcc ggcggaaaac
     2041 agctcacct
//
LOCUS       DATCZV010000122         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_644003, whole genome shotgun sequence.
ACCESSION   DATCZV010000122 DATCZV010000000
VERSION     DATCZV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644003"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VIW27_02740"
     CDS             <1..415
                     /locus_tag="VIW27_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEY5738611.1"
                     /translation="PWLFSLALGLIAPYSGTIGARVEEIGPGYARVTLRDRRRVRNHL
                     RSTHAIALINLGEIATGLAVLSTVSANMRGIVLGIEASFVKKARGKLTATADFRLPEL
                     VEENTSCEVTAQLRDESGETVAEVKATWLIGYQAS"
     gene            412..1401
                     /locus_tag="VIW27_02745"
     CDS             412..1401
                     /locus_tag="VIW27_02745"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY5738612.1"
                     /translation="MKPDLPDAAKRFCEQVGIRYPVIGGPMYPCSNPELTAAVSQAGG
                     IGILQPISLTYVHGHDYRAGIRLMRRLTDKPIGMNALIEKSSDKYRRRMSQWIDIALE
                     EGVRFFVTSLGKPDWVAERVHAAGGLVYHDVTELKWARIGLDNGADGLICVNNRAGGH
                     AGDKTMRALYEELHSLGAPLVCAGGIGESTGLWEALDCGYAACQLGTRFIASHECRAS
                     EAYKQAVINANEADIVLSERITGIPVSVINTDFIRRSGTRIGRFSRWMLSGNTTKHWM
                     RMFYGLKSIWQLKRSSLDETGEKDYWQAGKSVSGIDSIETCATIMQRLLSREV"
     gene            1440..2021
                     /locus_tag="VIW27_02750"
     CDS             1440..2021
                     /locus_tag="VIW27_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY5738613.1"
                     /translation="MCSWCWGFEPTRRQLFAALEGRLQIERLVGGLAPDTDEPMPATM
                     RNMLQQTWHRIAISIPGTEFNFEFWEKCQPRRATYPANRAVIAARSQGDEFDVRMTER
                     IQRAYYLEARNPSDDSTLIELAADIGLDCERFADDLASAALQRQLLQEIQRSRMLGID
                     SFPSLAVEKSGHLHHIDLNYTDAQAMRAQIEAL"
     gene            complement(2028..>2254)
                     /locus_tag="VIW27_02755"
     CDS             complement(2028..>2254)
                     /locus_tag="VIW27_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5738614.1"
                     /translation="TDVAALSAMIGKTSTTLEADSALLAGLTHSTGLSPILVKAENYP
                     DILGNAQQSVEEINMTGGVIEVEEIREAIL"
BASE COUNT          462 a          651 c          686 g          455 t
ORIGIN      
        1 gccatggttg tttagcctcg cgctcggttt aatcgcgcct tacagcggca cgatcggcgc
       61 ccgcgtcgag gagatcgggc cgggttatgc gcgcgtgacg ctgcgtgacc gccgccgggt
      121 gcgcaaccac ctgcgttcga cacacgcgat cgctctgata aacctgggtg aaattgcgac
      181 cgggctggcc gtcctgtcga ccgtatccgc taacatgcgc ggcatcgtgc tcggtattga
      241 ggccagcttc gtaaaaaagg cgcgcggaaa actcaccgcg actgccgatt ttcgattgcc
      301 cgagctggtc gaggaaaaca cctcctgcga ggtcacggcg cagctgcggg atgagtccgg
      361 cgagacggtc gccgaggtca aggccacctg gctgatcggt taccaggcat catgaaaccg
      421 gatttgcccg acgccgccaa aaggttttgc gaacaggtcg gcattcgcta cccggttatt
      481 ggcggtccga tgtacccttg cagcaatccc gagctcaccg ccgcggtttc gcaagccggt
      541 ggaatcggta ttctgcaacc tatctcgcta acctacgtcc acggccatga ttatcgcgca
      601 ggcatccgcc tgatgcgccg actgaccgac aagccgatcg gcatgaatgc gctgatcgaa
      661 aaatcgtcgg acaagtatcg gcgacgcatg tcgcaatgga tcgatatcgc gctcgaggaa
      721 ggtgttcgct ttttcgttac ctcgttgggt aagcccgact gggtcgccga gcgcgtgcac
      781 gccgcgggcg gcctggttta ccacgacgtc accgagctga agtgggcccg aatcgggctc
      841 gataacggtg ccgatgggct gatctgcgtc aataatcggg cgggcggtca cgccggcgat
      901 aaaacgatgc gcgcgcttta cgaggaattg catagcctgg gtgcgccgct ggtctgtgcc
      961 ggcggtattg gcgaatccac cggactatgg gaggcgctgg actgcggcta cgccgcctgc
     1021 cagttgggga cgcgctttat cgccagccac gagtgccgtg caagcgaagc ctacaagcag
     1081 gcggttatca acgctaacga agccgacatc gtgctgtcgg aaagaattac cgggattccg
     1141 gtgtcggtga tcaataccga ttttatcagg cgcagcggca cccgcatcgg acggttttcc
     1201 cgctggatgc tcagcggcaa caccacaaag cactggatgc gcatgtttta cgggttgaaa
     1261 tcgatctggc aattgaaacg ctcatcgctc gatgaaaccg gtgaaaaaga ctactggcag
     1321 gcgggcaaga gcgtgtccgg tatcgacagc attgaaacct gcgcaacgat catgcaacga
     1381 ttgctttcac gcgaggtcta aacggcatgc aggaaacact ctattacgtt cacgatccga
     1441 tgtgtagctg gtgctgggga ttcgaaccca cgcgccggca actgtttgcc gcactcgagg
     1501 gccggctgca aatcgaacgc ctggttggag gcctggcgcc cgatacggac gaaccgatgc
     1561 cggcaacgat gcgcaacatg ctacagcaga cctggcatcg cattgcaata tcgattcctg
     1621 gcaccgagtt taatttcgag ttctgggaaa agtgccagcc gcgtcgtgcc acttatccgg
     1681 caaaccgcgc tgtcatcgcc gcgcgctcgc agggcgacga attcgatgtg cggatgaccg
     1741 agcgaatcca gcgggcctac tatctggagg ctcgtaatcc ctccgacgac agcacgctga
     1801 tcgaactggc ggccgatata ggtctcgatt gcgagcgttt tgcggatgac ctggcgtcgg
     1861 ccgcattaca acggcagttg ctgcaggaaa ttcaacgtag ccggatgctc ggcatcgata
     1921 gcttcccgag tcttgccgtg gaaaaatcgg ggcatttgca tcacatcgac ttgaactata
     1981 ccgatgcgca ggcgatgcgg gcgcagatcg aggcgctctg aagcgcccta taaaatcgct
     2041 tcccggatct cttccacctc gattacgccg ccggtcatgt tgatttcttc gacgctttgc
     2101 tgcgcattgc cgagtatgtc gggataattt tcggccttga ccaggatagg ggagagtccg
     2161 gtcgaatgcg tcaaacccgc aagcagcgcg ctatcggctt ctagcgtagt cgaggttttg
     2221 ccaatcatgg cgctgagcgc ggccacgtcg gtgc
//
LOCUS       DATCZV010000123         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_379541, whole genome shotgun sequence.
ACCESSION   DATCZV010000123 DATCZV010000000
VERSION     DATCZV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379541"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VIW27_02760"
     CDS             complement(<1..483)
                     /locus_tag="VIW27_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5738615.1"
                     /translation="MSNDTPIGPVSGTVVPRYAGSATFCRLPELRDLPRCDVAVLGVP
                     FDAGTSYRPGARFGPQAIRQASRHLRTNFHPAYQTEPFKSLQVADAGDVACNPYHIEE
                     AIGQIEAAADDVLTRAAALVSLGGDHTIAVPLLRAINKVAGRVALVHFDAHLDTWDTY
                     F"
     gene            566..1117
                     /gene="def"
                     /locus_tag="VIW27_02765"
     CDS             566..1117
                     /gene="def"
                     /locus_tag="VIW27_02765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5738616.1"
                     /translation="MSVRTLLKMGDPRLLEISSPVDPIDPVALAPLLNDMWDTMTAAN
                     GAGLAAPQIGVLRRVVIFGYQRNSRYPDAPPVPETVLINPQITPLGEAREQGWEGCLS
                     VPGMRGLVPRFSEIRYRGYDQYGELIEREVSGFHARVVQHECDHLDGILYPRRIEDLR
                     YFGYLDALDQHGVTEPQPCDDEP"
     gene            1107..1745
                     /locus_tag="VIW27_02770"
     CDS             1107..1745
                     /locus_tag="VIW27_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY5738617.1"
                     /translation="MSPDAAGARGFALLASGAHFSCALLQALLRRHCRPSLLMLPHYP
                     PAPEADSPAIEIDAGPVAPIRRIAADIETVYAPRARQPESAALVAERGIEFLLVACWP
                     YLIDGEFAASAAGAALNLHPSLLPKYRGPDPLQQQLATADRRFGVTLHLLDGQYDHGA
                     IVAQAELDDGGENSSLQELERRCAELGALLFIDALHAWPHWKPQPQPDPRNA"
     gene            complement(1968..2540)
                     /locus_tag="VIW27_02775"
     CDS             complement(1968..2540)
                     /locus_tag="VIW27_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738618.1"
                     /translation="MSEWLIYDDFVERVQVRHLQAFSGLLAGVTDSRHSFQIGFERGK
                     IILLTYRLSKGLEALRLITGLRRARIAEHPIPTMHAGRDDLIDTGAVLEALAAGTAND
                     TTTITRVDQSATGLKTIDLNMPGPLDERRKRIIEAAALRHFGPIGVLLCEEQFEKPDG
                     DVRSVVIGIAREVDASEDDTRAFIQSVAGA"
     gene            2785..>3104
                     /locus_tag="VIW27_02780"
     CDS             2785..>3104
                     /locus_tag="VIW27_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5738619.1"
                     /translation="MQPESIEELLTIDAAHVWHPYSAMHSELPVYAVEAAGGTGFTLA
                     DGRRLIDGMSSWWCAIHGYNHPRLNQAVTDQLRGMSHVMFGGLTHRPAVRLVERLVEI
                     TPPEL"
BASE COUNT          581 a          982 c          973 g          568 t
ORIGIN      
        1 gaaataggta tcccaggtat cgaggtgagc gtcgaagtgc accagcgcca cgcggccggc
       61 gaccttgttg attgcgcgca acaagggcac cgcgatggtg tggtcgccgc caaggctgac
      121 cagcgccgcg gcgcgcgtca aaacgtcgtc ggcggcggcc tcgatctggc cgatggcttc
      181 ctcgatatgg tacggattgc aggcgacgtc gccggcgtcc gcgacctgca ggctcttgaa
      241 cggctcggtc tggtaggcgg gatgaaaatt ggtgcgcagg tgtcgcgaag cctggcgtat
      301 agcctgcggc ccgaagcgcg cgccgggccg gtaactggtg ccggcgtcga acggcacgcc
      361 gagcaccgcg acatcacagc gcggcaaatc ccgcagttct ggaaggcgac aaaaggtagc
      421 cgatccggca tagcgcggca ccactgtgcc gctgacggga ccgatcggag tatcgttgga
      481 cacttcgcta acctcgctgg cggagcgatt tgttatatca tcgatatctg gcacaatcaa
      541 atcgacagcc tttgcggagt ttcctgtgag cgtcagaacc cttctcaaaa tgggcgatcc
      601 aagattgctg gagatttcca gtccggtgga cccgatagac cccgtggcgc tggcgccgtt
      661 gctcaacgac atgtgggata cgatgacggc ggccaatggc gccgggcttg ccgcaccgca
      721 gatcggcgtg ctgcggcgcg tggtgatctt cggctaccag cgcaattcgc gttatcccga
      781 cgcaccgccg gtgcccgaga cggtactcat caatccgcag attacgccgc tcggcgaggc
      841 ccgcgaacag ggctgggagg gctgtctcag cgttcccggc atgcgcggtc tggtgccgcg
      901 attcagcgaa attcgctacc ggggttacga ccagtacgga gaattgatcg agcgcgaggt
      961 ctcgggtttt cacgcgcgcg tggtgcagca cgagtgcgac cacctcgacg gtatcctgta
     1021 cccacggaga atcgaggacc tgcgttactt cggttacctc gacgcgctcg atcagcacgg
     1081 cgtgaccgag ccgcaaccct gcgacgatga gccctgacgc ggcaggcgca cgcggcttcg
     1141 cattgctggc cagcggcgcg catttttcgt gcgcactgtt acaggccttg ctgcgcaggc
     1201 actgccggcc gagtctgttg atgctgccgc attacccgcc ggcccccgaa gccgattcgc
     1261 cggccatcga aatcgatgcc ggacccgtgg cgccgatacg gcgtatcgcc gcggatatcg
     1321 aaaccgtcta cgcgccgcgg gcgcggcagc ccgaatcggc ggcactggtc gccgaacgcg
     1381 gcatcgaatt cctgttggtg gcttgctggc cttacctgat cgatggcgag ttcgccgcca
     1441 gtgccgccgg cgccgcgctc aacctgcatc cgtcgctgct gccgaagtat cgcggtcccg
     1501 atccgttgca gcagcagctg gccaccgcgg accggcgatt cggcgtaact ctgcacctgc
     1561 tcgacgggca atatgatcac ggcgccatcg tcgcccaggc cgaactcgac gatggcggcg
     1621 aaaacagcag cctgcaggaa ctggaacgac gctgcgccga actcggcgca ctgctgttca
     1681 tcgacgcgct gcacgcctgg ccgcattgga aaccacaacc ccagccagac ccgcgaaatg
     1741 catgagcatc acgcgacgcg gcgcgcgcca atcttcaccg ccggactctt cgcggtggca
     1801 ggcatttggt cagcatggcg acaacggcag attcgaatgc accactcgca ccggccggca
     1861 acctacggtt actcctcgag gaagttgatt tcggtttcgc taatggccag gacggcttgc
     1921 cgcgcaaagc cgttctccgg cctggtcaga tccctacagg ataggggcta ggccccggca
     1981 accgactgta taaaggcccg cgtatcgtcc tcgctggcat cgacttcgcg cgcgatgccg
     2041 atgacaaccg acctgacatc gccatcgggc ttttcgaact gctcctcgca aagcaatacg
     2101 ccgatcggac cgaaatgcct caacgcggcg gcttcgatga tccgtttgcg gcgctcgtcg
     2161 agcggtcccg gcatgttcag atcgatcgtt ttcaagcccg tcgcggattg gtcgacgcgc
     2221 gtgatcgtcg tggtgtcgtt ggcggtcccg gcagccagcg cctcgagcac cgcaccggtg
     2281 tcgatcaagt catcgcgacc ggcatgcatc gtcggtatcg gatgctcggc gatccgcgcg
     2341 cgtcgcagac cggtgatcag cctgagcgct tccaggccct tgctgaggcg gtaggtcagc
     2401 agaatgatct tgccgcgctc gaaaccgatc tggaaggagt gtcggctgtc ggttaccccg
     2461 gcaagcaggc cgctgaatgc ctgtagatga cgaacctgga cgcgttcgac gaaatcgtcg
     2521 taaatcagcc actcgctcat cgcatcagcc gccgtttacg ggttgcggga tcggcatgct
     2581 agtcatcagc gggcaaatcc tgtagtttga cgagcgcggc ggcaaccgtc actgccgatc
     2641 ggacacgaat ccggatcgac gggaattgtt tcatgcaagt aatcaacgac atcgaaccct
     2701 gcttatgata attatcgaat cggcgcatgc cgggaaatcg gctgttaacc tgtcccccag
     2761 gccacccgaa attttgaacc aatgatgcaa cccgagtcaa tcgaagaact gctgacaatc
     2821 gatgccgcgc atgtctggca cccctacagc gcgatgcaca gcgagttgcc ggtctatgcc
     2881 gtcgaagcag ccggcggtac gggatttacg ctggccgacg gccgccgcct gatcgatggc
     2941 atgtcgtcct ggtggtgtgc gattcacggc tacaaccacc cgcggctcaa ccaggcggta
     3001 accgatcagt tgcgaggcat gtcgcatgtc atgttcggcg gtctgactca ccggccggcg
     3061 gttcggctgg tagaacggct ggtggaaatc acgccgcctg agct
//
LOCUS       DATCZV010000124         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_167821, whole genome shotgun sequence.
ACCESSION   DATCZV010000124 DATCZV010000000
VERSION     DATCZV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167821"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VIW27_02785"
     CDS             complement(<1..317)
                     /locus_tag="VIW27_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEY5738620.1"
                     /translation="MSVRVIACLALLSLLAVLPPAMAGVEVSVDRNPVRLNDTFQLVF
                     ELDENPDRDPDFSSLREDFLVLGNNRSSSISIINGNYRRSVKWTLQLMAKDVGEFEIP
                     AVR"
     gene            complement(314..2155)
                     /locus_tag="VIW27_02790"
     CDS             complement(314..2155)
                     /locus_tag="VIW27_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5738621.1"
                     /translation="MIEQFHFLRPGWLLLLLPLAAWLWGLFRGRYDHGNWRAVVDERL
                     MPYVLSGSGGARGHGVRALPGAAAGLAIIALAGPTWEKLPQPVYQTETALVIMLDLSR
                     SMDAADIKPSRLVRARHKIADILNLRTEGQTGLVVYAADAFAVTPLTSDTETVMAMLP
                     NLDSSLMPAQGSRADRAVERALALFENAAHKRGDLLLISDGIGDSELQRVEALLARHA
                     EFRLSVLAVGTVDGGPIPLASGGFLKTGDGAIVISSMQEDNLRRLADHGGVYATLSSD
                     DIDINTLAYVFEASIDSSAARRADDRRTEVWRELGPWLLLAALPLAALAFRRGLFWLL
                     PLWLVVVPPNADAIDWQALWQNQDQRASGLFAAEDYAAAAEIFDDPAWKASSQYRAGD
                     YAGALDNWNAVDSDQALYNRGNALARLQRYDDALAAYDALLERNPEHRDALFNKRAIE
                     EFLRNRQQQQQQQSGEGGEQQQSQDQQQQQSQGGPQQQSQNGQQGQQQGDDEQQGAGA
                     GQPQSPAGEPQDDDQRQNAGQDGEQQRERQDDAGEQGSSEAGQGSESETAAPGAEPRE
                     GIDEQMSAQAAEQWLRKIPDDPGGLLRRKFLYQYRQRGTVDDEAESW"
     gene            complement(2152..>2801)
                     /locus_tag="VIW27_02795"
     CDS             complement(2152..>2801)
                     /locus_tag="VIW27_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5738622.1"
                     /translation="VNRLQATKQVASRFIERRRGDRLGLILFGTRAYLQAPLTFDNQT
                     VIRLLRESALGLAGERTAIGDAIGLAIKRLDLESESSRVLVLMTDGANTAGEVAPQKA
                     AQLAADRGLKIYTIGIGADQDIETSWFGIRRRNPSAQLDEESLRAIAQVSGGRYFRAR
                     DSEELEQIYRELDALEPMPRDSEKLRPASALFVWPLGIALALAAVLALPGFGRLR"
BASE COUNT          443 a          954 c          903 g          501 t
ORIGIN      
        1 aagcgaaccg ccggtatctc gaattcaccg acgtccttgg ccatcaattg cagcgtccat
       61 ttgacgctgc gccgataatt gccgttgatg atgctgatgc tgctgctgcg attgttgccg
      121 agcaccagga aatcctcgcg cagcgaagaa aaatcgggat cgcgatccgg attctcgtcg
      181 agctcgaaca ccagctgaaa ggtatcgttg aggcgcaccg ggttacggtc gacgctgacc
      241 tcgaccccag ccatcgccgg tggtaagacg gccagcagcg atagcaatgc caggcaggcg
      301 ataactcgta cgctcaccat gactcggctt cgtcgtcgac agtgccgcgc tggcggtact
      361 ggtagagaaa cttgcggcgc aacaatccgc cggggtcgtc gggaatcttg cgcaaccact
      421 gctccgcggc ctgcgccgac atttgctcgt cgatgccctc gcgcggctcg gcccccggcg
      481 ctgcggtttc gctctcgctg ccctgcccgg cctcggacga cccctgttcc ccggcgtcat
      541 cctgccgctc gcgctgctgc tctccgtcct gccctgcatt ttgccgctga tcgtcatcct
      601 gcggttcgcc cgctggagat tgcggctgcc cggcgccagc gccctgctgc tcgtcatcgc
      661 cctgttgctg cccctgctgc ccattctgcg actgttgctg tggcccgccc tgcgattgtt
      721 gctgttgctg atcctgcgac tgttgctgct ctccaccctc gccggactgc tgctgttgct
      781 gctgttggcg gttgcgcaga aattcctcga tcgcgcgctt gttgaacagc gcgtcccggt
      841 gttcggggtt gcgttccagc agcgcgtcgt aggcggccag cgcatcgtcg tagcgttgca
      901 ggcgggcaag cgcattgccg cgattgtaga gcgcctggtc gctgtcgaca gcgttccagt
      961 tatcgagcgc gcccgcgtag tcgccggcgc ggtattgcga cgacgctttc caggccggat
     1021 cgtcaaagat ttccgcggcc gccgcgtaat cctcggccgc gaacaaacca ctggcacgct
     1081 ggtcctggtt ttgccacagc gcctgccagt cgatggcgtc ggcgttcggc ggcaccacga
     1141 caagccatag cggcagcagc cagaacaggc cgcggcgaaa cgccagcgcc gccagcggca
     1201 gcgccgcgag cagcagccac ggacccaatt cgcgccacac ttcggtccgg cggtcgtcgg
     1261 cgcggcgcgc cgcgctcgaa tcgatgctcg cttcgaatac gtaggccagc gtattgatat
     1321 cgatgtcatc gctgctgagt gtcgcgtaga caccgccgtg gtcggcgaga cggcgcagat
     1381 tgtcctcctg catcgacgaa atcacgatcg caccatcgcc ggttttcaga aaaccaccgc
     1441 tggcgagcgg tatcggtccg ccgtcgacgg taccgacggc cagcaccgac aggcgaaact
     1501 cggcatgcct ggcgagcagc gcctcgacac gctgcagctc gctgtcgccg atcccgtcgc
     1561 ttatcagcag caggtcgccg cgcttgtggg cggcgttctc gaacaatgcg agcgcgcgct
     1621 cgacggcacg gtcggcgcgg ctgccctgcg ccggcatcag cgagctgtcg agattcggca
     1681 gcatcgccat cacggtctcg gtatcgctgg tcaacggtgt aacggcaaag gcgtcggcgg
     1741 cgtagacgac cagccccgtc tgcccttcgg tgcgcagatt gagaatatcg gcgatcttgt
     1801 gacgggcacg gaccaggcga ctgggcttga tgtcggcggc gtccatcgag cgcgacaggt
     1861 cgagcatgat caccagcgcg gtctcggtct gataaaccgg ttgcggtagc ttttcccagg
     1921 tcgggccggc cagtgcgata atcgcgagcc cggcggccgc gcccggcagc gcgcgcacgc
     1981 catgcccgcg cgcgccgccg ctgccgctga gcacatacgg catcaggcgc tcgtcgacca
     2041 ccgcgcgcca gttgccatgg tcgtagcggc cgcgaaacag tccccacagc catgccgcca
     2101 gtggtaacag caacagcagc cagcccggcc tgaggaaatg aaactgctcg atcatcgcag
     2161 cctgccgaag ccgggtaacg cgagcaccgc ggccaacgcc agcgcgatgc ccagcggcca
     2221 gacgaacagc gcgctcgccg ggcgcagctt ttcgctgtcg cgcggcatgg gttcgagagc
     2281 atcgagctcg cggtagatct gttcgagctc ctcgctgtcg cgggcgcgaa agtaacgccc
     2341 gccgctgacc tgcgcgatcg cgcgcaggga ttcctcgtcg agttgcgccg acgggttgcg
     2401 gcgtcgaatc ccgaaccagc tggtttcgat atcctggtcg gcgccgatgc cgatggtgta
     2461 gatcttcagg ccgcgatcgg cggccagctg cgcggccttt tgcggcgcca cctcgccggc
     2521 ggtattggcg ccgtcggtca tcagtaccag cacccgcgaa ctctccgatt cgagatcgag
     2581 gcgcttgatc gccagcccga tggcgtcgcc gatcgcggta cgctcgccgg cgagcccgag
     2641 cgccgactcg cgcagcagcc gaatcacggt ctggttgtca aaggtcagcg gcgcctgcag
     2701 gtaagcgcgg gtgccgaaca ggatcagccc caggcggtcg ccgcggcggc gctcgatgaa
     2761 gcggctggcg acctgcttgg tcgcctgcag gcggttgacc t
//
LOCUS       DATCZV010000125         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_749774, whole genome shotgun sequence.
ACCESSION   DATCZV010000125 DATCZV010000000
VERSION     DATCZV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749774"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIW27_02800"
     CDS             <1..190
                     /locus_tag="VIW27_02800"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005522574.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase"
                     /protein_id="HEY5738623.1"
                     /translation="DNFSLPQLRAAVALNARRIELEASGNIDSGNLREVAETGVDYVS
                     IGALTKHLQAIDFSLRYR"
     gene            391..1329
                     /locus_tag="VIW27_02805"
     CDS             391..1329
                     /locus_tag="VIW27_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5738624.1"
                     /translation="MAADSRPAEAQQDQVGKGILALILATLLFATQDAITKHLTASMP
                     IAQLMFVRYCFFALFALVFATRKLGLRAAFRSHSPRLQVLRGLLIAVEMALFAFCLKF
                     LGIAEMHALFACFPLMITGLSVPLLGERVGWRRWLAVAVGFAGTLIIVQPGSGVISSY
                     ALIALVCALMFAFYNIVTRKVSRRDNFETSLLYFGLVGLAATSLAVPFFWQTPRFEDA
                     VWLLAISIISIFSHLLLIKSLQLAPAVILQPFNYFILVWAMIIGYLFYGEILESATLA
                     GASLVVASGIYIARREYRVARTERRRLRRAMYPPEV"
     gene            1539..>3015
                     /locus_tag="VIW27_02810"
     CDS             1539..>3015
                     /locus_tag="VIW27_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEY5738625.1"
                     /translation="MSLLLWGLRMVRIGISDGWGEEIRATLGASLNNRLKALATGLGV
                     TMLLQSSSATALLAASFSGQGILTTATALAILLGADVGTSLVAQIFTFDLSILSPLLI
                     TLGVTLYTYFPGGRARDFGRLMLGIGLMLLALKLIIAASSPMRESVIVQEIFLALDQD
                     LVLAVLFAAIVAWASHSSLATVLLVISLAHTSALDLRVAFAFVLGANLGSAIPPLVAT
                     LGANHGARQPPLGNLLFRLCGVVIALPFVDLAVHQLAPLEVGNARQIANFHLLFNIAL
                     AVLFFPLIGTMAKLTERLLPSEDPLVDGLQPKKLDRGAFQTPLVALVNAERETLRMGE
                     VVERMLRNAFVALKKNDAALAERTQDMDLLANQFYDNIKEYLTALAREPLGAKESRRC
                     TEIISFATNLEHIGDIISGDLIDNIVSKKVAHRSKMSLQDREDLNNLYQPVLRAFDLS
                     LNVFTSGDVAMARTLLANKYKFIKRENRAVMNHLEKIRDDST"
BASE COUNT          539 a          953 c          886 g          637 t
ORIGIN      
        1 cgataatttt tctctgccgc aactgcgcgc ggccgtggcg ttgaacgcgc ggcgcatcga
       61 gctcgaggct tcgggcaaca tcgatagcgg taacctgcgc gaagtcgcgg agaccggcgt
      121 cgactacgtg tcgatcggtg cgctgaccaa gcacctgcaa gcgatcgact tctcgctgcg
      181 ttaccgctga tcgcggttcg cgacgctggc gatccatacg caccgtctat cgcaaccgtc
      241 ggaaaataca attattccaa tgctcggcaa cgcagtaatc tggcggtatt caatccaggc
      301 cgaaaaccat gcgtaatcca agtcccgggc tgctggcatg cccttttagc gctacagaaa
      361 agactcctcc cacggcgcgc ggatcccggt ttggcggccg atagtcgccc cgccgaggct
      421 caacaggacc aggtcggcaa gggcatactg gcgttgatcc tggcgaccct gttattcgcg
      481 acccaggacg cgatcaccaa gcacctgacg gcctcgatgc cgatcgcgca actaatgttc
      541 gtgcgctatt gttttttcgc gctgttcgcg ctggttttcg cgacgcgcaa gctcggcttg
      601 cgggccgcgt ttcgttccca cagcccgcga ctgcaggtgc tgcgcgggct gctgatcgcg
      661 gtagagatgg ccctgttcgc cttttgcctg aagtttctcg gcatcgccga gatgcatgcg
      721 ctgttcgcct gcttcccgct gatgatcacc ggtctctccg tgccgttgct cggcgagcgc
      781 gtcggctggc gccgctggct cgcggtagcg gtcggctttg ccggcacact gatcatcgtg
      841 cagcccggca gcggcgtgat ttcgagctat gcgctgatcg cgctggtctg cgcgctgatg
      901 ttcgcttttt acaacatcgt cacgcgcaag gtgtcgcgtc gcgacaactt cgaaacctcc
      961 ctgctctact tcggcctcgt cggcctcgcg gcaacctcgc tggcagtccc ctttttctgg
     1021 caaaccccgc ggttcgaaga cgctgtctgg ctactcgcaa tcagcattat ttccatcttc
     1081 agtcacctgc tgctgatcaa atcactgcag ctggcgccgg cagtcattct gcaaccgttt
     1141 aattatttca tcctcgtctg ggcgatgatc atcggttacc tgttttacgg cgaaatcctg
     1201 gaaagcgcga cgctggcggg tgcgtcgctg gtcgtcgcca gcggcatcta tatcgcgcgg
     1261 cgcgagtacc gggtggcgcg caccgaacgc cgccgcctgc gcagggcgat gtacccgccg
     1321 gaggtctgag ttgcaggagt ggggagttag acgccgatga aaggcgtcga ccgggggtca
     1381 caaaatgata attgttgtat ataatatgta tatacatcgt ggtagtttac caaatgcacc
     1441 tatactcatc gaaatgaagt acttcgaatc ctgccggatt tccggcgcga ggttgcgcca
     1501 ttagcggcag cagcatactc gtcaacctga tcggcggcgt gtccctgctg ctgtgggggc
     1561 tgcgcatggt tcgcatcggc atctccgacg gctggggcga agaaattcgc gccaccctcg
     1621 gcgcctcgct gaacaatcgc ctcaaggccc tggctaccgg cctcggggta accatgctgt
     1681 tgcaaagcag cagcgcgact gcgttgctcg ccgcttcctt cagcggccag ggcattctaa
     1741 ccaccgcgac cgcgctggcg atattgctcg gcgccgatgt cggcaccagc ctggtcgcgc
     1801 agatattcac gttcgacctg tcgatactgt cgccgttgct gatcacgctg ggtgtcacac
     1861 tctacactta tttcccgggc ggccgggcac gcgacttcgg tcggctgatg ctcggcatcg
     1921 ggctgatgct gctggcgctg aaactgatca tcgcggcatc gtcgccgatg cgggaatcgg
     1981 taatcgtgca ggagatattt ctcgcgctgg atcaggatct ggttttggcg gtcctgttcg
     2041 ccgcaatcgt cgcctgggcc tcgcactcaa gcctggcgac cgtattgctg gtgatttcgc
     2101 tggctcacac cagtgcgctc gatctgcggg ttgcattcgc attcgtgctc ggcgccaacc
     2161 tcggcagcgc gattccaccg ctagtcgcca ccctgggcgc caatcacggt gcccggcagc
     2221 cgccgctcgg caacctgctg tttcgccttt gcggggtggt gatcgcgctg ccgtttgtcg
     2281 acctggccgt gcaccagctt gcgccgctcg aagtcggcaa cgcgcggcag atcgccaact
     2341 ttcacctgct gttcaacatt gcgctggcgg ttctgttctt tccgctgatc ggcaccatgg
     2401 ccaagcttac cgagcgtcta ctgcccagcg aagatccgct cgttgatggg ctgcagccga
     2461 aaaaactcga tcgcggcgcc tttcagaccc cgctggtggc actggtcaac gccgagcgcg
     2521 aaaccctgcg catgggcgag gtcgtcgaac gcatgctgcg caacgcgttc gtggcgctga
     2581 agaagaacga tgccgccctc gccgagcgca cccaggacat ggacttgctg gcgaaccagt
     2641 tctacgacaa tatcaaggag tacctgaccg cgctggcccg cgaaccgctc ggcgccaagg
     2701 aaagccggcg ctgcaccgag atcatcagct tcgcgaccaa cctggagcac atcggggata
     2761 tcatttcggg cgacctgatc gacaacatcg tcagcaaaaa agtcgcccac cgcagcaaga
     2821 tgtcgctgca ggaccgcgag gatctgaata atctctacca gccggtgctg cgagccttcg
     2881 acctgtcgct gaacgtcttc acctcggggg acgtggccat ggcccgcacg ctactggcaa
     2941 acaagtacaa gttcatcaag cgcgagaacc gggcggtcat gaaccacctc gaaaaaattc
     3001 gcgatgacag cactt
//
LOCUS       DATCZV010000126         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_644195, whole genome shotgun sequence.
ACCESSION   DATCZV010000126 DATCZV010000000
VERSION     DATCZV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644195"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="VIW27_02815"
     CDS             complement(<1..1839)
                     /locus_tag="VIW27_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEY5738626.1"
                     /translation="MSKSSKHTRQGTRLILEELEQRRLFSGGAEGVLGIGDELDKDSL
                     FQDIDAYEAPLDDSGENAAQAEERSHEVVFVDAGVDDYQLLVDDIRANMDLSRELEVV
                     VLERDRDGIAQISEALKAYNDLDAIHIVSHGSDGSVRLGNASLNTETLEQNNLSIALW
                     ANAFSDIGDILFYGCNLAATENGQSLIDELGALTLTDVAASSDLTGVASKGGDWDLEF
                     RAGEIETAVVLGLDGQQTWDGLLVVPIITARETVDSDGDGQIDYIRMTTEDGNGNPVN
                     VNDKFDNIVVTVVDGAYNYAVTGYQTSIPSEAGAGNDNVFYVALTERGTPDTGATPTV
                     TVVSNTKLKEDAGKGSDTLPVDVVGVVATDDVAPILISASTNVLDGQQILDQQGESLT
                     LVFSESLSGAPTEAQLEAALSFAGGATDGNNLPDIGTGANPISLATTTVANDSVVVTF
                     NANNVAAADPVTAGIHTVSVGDGSNLTDTSGNTANTTLPAVVIDGGANPLWIVSRETA
                     DLDGDGFIDAIHVTFSVAVDDSTIFATDWDVFGVTGEAFVSTTNGDTADDADIYITFA
                     DGVLDTAAIPQISYTQGTLTDTLGNPLSDDLGIDWLDTSWLQRTKFA"
     gene            complement(1850..2791)
                     /locus_tag="VIW27_02820"
     CDS             complement(1850..2791)
                     /locus_tag="VIW27_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5738627.1"
                     /translation="MAVTKQSLNYIHASRRERRSASYRARTARAVAVLLALIACTPAV
                     ARAPAELDCLVKPEMYVDVSSPVISVLEEILVETGETVVKGQPLAKLEASVEKARVKL
                     ARLQAESTSEVENRKEQYRYAKQYFRRMQDLLAQKSVSQYEADKAQTELALAQIDLNR
                     ARENRRIAQVNLELAQSQLALRTIRSPIDGIVVDRYTMVGESVEGRVIMKLAQIDPLK
                     VELIAPTEYFGLISKEMKVDIYPEQPAGQAFEATVNVVDQLIDPASGSFTVRMELPNP
                     DDKLVGGVNCLARFDFETPKPSSGEVFSSLPDSVMAD"
     gene            complement(2791..>2970)
                     /locus_tag="VIW27_02825"
     CDS             complement(2791..>2970)
                     /locus_tag="VIW27_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738628.1"
                     /translation="LKFFSRLAGNSKPLPKLIAGALLRRAQNRHERQQCASRKALTRQ
                     DRRLRRILAFAGRFE"
BASE COUNT          519 a          897 c          924 g          630 t
ORIGIN      
        1 cgcgaacttg gtgcgctgta accatgatgt atccagccag tcgatgccca ggtcatcgga
       61 aagcggatta cccaacgtat cggtgagcgt gccctgggtg taggaaatct gcggaatcgc
      121 ggccgtgtcg agcacgccgt cggcgaaagt gatgtagatg tcggcgtcgt cagcggtgtc
      181 gccattggta gtcgagacga atgcttcgcc ggtgacgcca aagacgtccc agtcggtcgc
      241 gaaaatggtc gagtcatcga ccgccacact gaaggtgacg tggatggcgt cgatgaaacc
      301 atcgccgtcg aggtcggccg tctcccgcga cacgatccac agcgggttgg ccccgccgtc
      361 gatgaccacc gccggcaggg tggtattggc ggtgttgccc gaggtgtcgg tcaaattgct
      421 gccgtcgcca acgctcacgg tatggatacc ggcagtcacg gggtcggccg cggcgacgtt
      481 gttggcgttg aaggtcacca cgacgctgtc gttggcgact gtggtggtag ccagtgatat
      541 cgggttggcg ccagtgccaa tgtcaggcag gttgttgccg tcggtagcgc cgccggcaaa
      601 gctgagcgcc gcttcgagct gcgcttcggt gggcgcgccg ctgaggcttt cactgaacac
      661 cagggtcagc gactcgccct gctgatccag gatctgctgg ccatcaagaa cgttggtgct
      721 ggcggaaatc aggatcggcg caacgtcatc ggtggcgacg acacccacga cgtccacagg
      781 cagcgtgtcg gagcctttgc ctgcatcttc cttcagcttg gtgttcgaaa ccaccgttac
      841 cgtcggtgtc gcgccggtgt cgggggtgcc tctctcggtc agcgcgacgt aaaagacgtt
      901 gtcgttgcca gcacccgctt cgctcggaat actggtctga tagccggtaa cggcgtagtt
      961 gtaggcaccg tccaccaccg tgacaacgat attgtcaaat ttgtcgttta cattgaccgg
     1021 attaccgtta ccgtcttcgg tggtcatacg gatgtaatcg atctgtccgt cgccgtcgct
     1081 atccaccgtc tcgcgtgccg tgataatcgg gacgaccaat agcccgtccc aggtttgctg
     1141 gccatcgagg cccagcacga ccgcggtctc gatctcgccg gcccggaatt cgaggtccca
     1201 gtcgccgccc ttgctggcga cgccggtcag gtcgctggaa gcggcaacat cggtgagtgt
     1261 cagcgcgccg agttcgtcga tcaggctctg gccattttcg gtcgccgcca ggttacagcc
     1321 gtagaacagg atgtcaccga tatcgctgaa ggcattggcc cacagcgcga tgctgagatt
     1381 gttttgctcg agcgtctcgg tattgaggct ggcattgccg agccgcacgc tgccgtcact
     1441 gccgtgggaa acgatatgga tggcgtcgag gtcgttgtaa gctttcaagg cctcggatat
     1501 ctgcgcgata ccgtcgcgat cgcgctccag caccacgact tcgagttcgc ggctcaaatc
     1561 catgttggcg cgaatgtcgt cgaccagcag ctggtaatcg tcgacgccgg catcgacgaa
     1621 gacgacttcg tgactgcgtt cctcggcctg cgcggcattc tcgccactgt cgtcgagcgg
     1681 tgcctcgtag gcgtcgatat cctggaacag cgaatccttg tcgagctcgt cgccgatacc
     1741 cagcacgcct tccgcgccgc ccgagaacag gcgccgctgc tcgagttctt cgagtatcag
     1801 ccgggtgcct tgccgggtat gtttcgagga tttgctcata ttgtgccgtt taatccgcca
     1861 tcaccgaatc gggtagcgaa ctgaatacct cgccgctcga cggtttcggg gtttcgaagt
     1921 cgaagcgtgc caggcagttg acgccaccga ccagcttgtc gtccgggttc ggcaactcca
     1981 tgcgcacggt aaagctgccg ctggccgggt cgatcaattg atcgaccacg ttgacagtgg
     2041 cctcgaacgc ctggcccgcc ggctgctccg ggtaaatatc gactttcatc tccttgctga
     2101 tcagcccgaa gtactcggtc ggcgcgatca actcgacttt caacggatcg atctgggcga
     2161 gtttcatgat cacccggccc tcgaccgatt cgccgaccat cgtgtaacga tcgacgacga
     2221 tgccatcgat cgggctgcga atcgtgcgca gcgccagctg cgattgcgcc agctcgagat
     2281 tgacctgggc gatgcgccgg ttttcgcgcg cgcgattgag atcgatctgc gccagcgcga
     2341 gctcggtctg cgccttgtcg gcctcgtact gcgaaaccga tttctgcgcc agcagatcct
     2401 gcatgcgccg aaaatactgc ttggcatagc ggtactgctc cttgcgattc tcgacctcgc
     2461 tggtcgactc cgcctgtagg cgtgcgagct tgacgcgcgc cttctctacc gaggcttcga
     2521 gtttcgccag tggctgcccc ttgacgacgg tttcaccggt ctcgacgagg atctcttcga
     2581 gcaccgaaat taccgggctg ctgacatcga catacatctc cggcttgacc aggcaatcga
     2641 gttccgccgg ggcgcgagcg acggcgggtg tgcaggcgat caaagccagc aacacggcga
     2701 cggcgcgcgc ggtgcgggcg cgatagctgg ccgatcgccg ctcgcggcgg gatgcgtgga
     2761 tgtaattgag cgattgcttg gtaactgcca ttattcgaat ctccccgcaa aggccagtat
     2821 gcggcgcaac cggcgatctt gcctggttaa cgccttgcga ctggcgcact gctgtctttc
     2881 gtgacggttt tgcgcgcggc gcaacagggc gccggcgatt aatttgggca acggcttgct
     2941 attgccagcc agcctgctga aaaacttcag
//
LOCUS       DATCZV010000127         3719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_167914, whole genome shotgun sequence.
ACCESSION   DATCZV010000127 DATCZV010000000
VERSION     DATCZV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167914"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VIW27_02830"
     CDS             <1..275
                     /locus_tag="VIW27_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEY5738629.1"
                     /translation="PFILWIKDLSVMDPYYVLPIVMGISMVIQQKLNPPPPDPIQAKV
                     MMALPFVFTIFFAFFPAGLVLYWVVNNVLSIAQQWVITKRIEAGGK"
     gene            304..1638
                     /gene="mnmE"
                     /locus_tag="VIW27_02835"
     CDS             304..1638
                     /gene="mnmE"
                     /locus_tag="VIW27_02835"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY5738630.1"
                     /translation="MPAEDTIAAIATPPGTGGIGILRISGPDARSIGESISASHLEPG
                     LIQFRRFYDADGEVIDHGLCLYFAAPHSFSGEDCVELQAHGGAIVLDMLLERLCELGT
                     RLARAGEFSERAFLNGKLDLTQAEAIADLIESGSRAASRAAMRSLEGRFSKQVRQLVE
                     TVIELRVYVEAALDFADEEIDFLEDDAIGDRLADCRRNLETLLEQAEQGRRLQEGLTV
                     ALAGLPNAGKSSLLNYLAGYEAAIVTDIEGTTRDVLREHISLKGIPLRVYDTAGLRET
                     ENPVEKEGVRRAWNAIEQADVVLYLVDSSRGMSDADRRIIATLPRNRLQLVYSKSDLL
                     ATAPADEAGAIAVSVRDGAGTERLIERITGHTDDLNQDNQTFMARRRHVEALKRAAQS
                     LDQATTTFAASRSGELMAEDLRDAQRQLNEITGEFTSDDLLGRIFASFCIGK"
     gene            complement(1649..2617)
                     /locus_tag="VIW27_02840"
     CDS             complement(1649..2617)
                     /locus_tag="VIW27_02840"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY5738631.1"
                     /translation="MSVFTPIKQPQLEAFLERYDIGRLRAFAPIAAGITNTNYTVDTE
                     GGAFVLTLYEHHSDDELYYMLGLQRHLAARSVACAAPVGDRRGDFFSTLNQRPAAIIT
                     RLPGEVQPQPGIAHCAAVGAELARFHSAGMDFAARRINPRGADWVFAVSDMLDAELDD
                     SDRLLIASTLREYRGLDFESLPAGAVHADLFHDNVLFDGERLCGICDFDYACHDSLVL
                     DIAIVLHDWCRGASGELDEARGGALLGAYRHERELSRPEISALSAMLRFTALRFWLSR
                     HYDRLFPLPGELTYSKDPDEYRRLLLMCRDDGEILQQFLARFLEPA"
     gene            2706..>3719
                     /locus_tag="VIW27_02845"
     CDS             2706..>3719
                     /locus_tag="VIW27_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY5738632.1"
                     /translation="MNQENQKKLVLVDGSSYLFRAYHTRLSQMMTSPTGFPTQAIFIV
                     VNMLRKLLKDEQPDRVAVVFDAKGKTFRNDIYPEYKANRPPMPDDLREQIEPLHEIIR
                     AQGLPLICVEGVEADDIIGTLSREAVEQGYRVLISTGDKDLAQLVNDKVTLINTMSNE
                     SLNIDGVREKFQVGAEQIVDYLALMGDSSDNIPGVPKVGPKTAAKWIAEFGSLQNVMD
                     HADQIGGKVGENLRESLDFLPMSYQLATVKLDCDTGTTIDELEQRDPDPAVLAALYRE
                     YGFNRWLEELGAASETSHGQTAQPEANYECVLTRERFDHWLERLREANGFAVDTETTS
                     LDYM"
BASE COUNT          788 a         1141 c         1156 g          634 t
ORIGIN      
        1 caccgttcat cctctggatc aaggacctgt cggtcatgga cccctactac gtgctgccga
       61 tcgtgatggg tatcagcatg gtcatccagc aaaagctcaa tccgccgccg cccgacccga
      121 tccaggccaa ggtgatgatg gcattgccgt tcgtgtttac gatcttcttc gccttcttcc
      181 ccgccgggct ggtgttgtac tgggtcgtca acaacgtgtt gtcgatcgcg cagcagtggg
      241 tcatcaccaa gcgcatcgaa gccgggggca agtaagctcc ggtttcgcgg caacgcccag
      301 gccatgcccg cagaagatac tatcgccgcg atcgccacgc ctcctggcac tggcggcatt
      361 ggcatactcc gaatctcggg acccgacgcg cggtccatcg gcgagtcgat cagcgccagc
      421 cacctggagc ccggactgat ccagtttcgc cgtttttacg atgccgacgg cgaagtcatc
      481 gatcatggac tgtgcctgta cttcgccgcc ccgcacagct tcagcggcga ggactgtgtc
      541 gaattgcagg cccacggcgg cgcgatcgta ctcgacatgc tgctcgaacg gctgtgcgaa
      601 ctcggaacga ggctggcgcg cgccggcgaa ttttccgagc gagcattctt gaacggcaag
      661 ctggacttga cccaggccga ggcgatcgcc gacctgatcg aatcgggcag ccgcgcagcc
      721 agccgcgcgg cgatgcgctc gctcgagggc cgcttctcga aacaggtcag gcagctggtg
      781 gagacggtca tcgaactgcg cgtttacgtc gaggccgcgc tcgatttcgc cgacgaggaa
      841 atcgatttcc tcgaagacga cgcgatcggc gatcggcttg ccgactgccg gagaaacctg
      901 gaaaccctgc tcgaacaggc cgaacagggc cgcagactgc aggaagggct gaccgtggca
      961 ctcgccgggc tacccaatgc cggcaagtcc agcctgttga attacctcgc cggatatgaa
     1021 gccgcaatcg tcaccgacat cgagggtacc acgcgcgatg tgttacgcga gcacatctcg
     1081 ctaaaaggca taccgctgag ggtgtatgac acggcgggcc tgcgagaaac cgaaaacccg
     1141 gtggaaaagg agggcgtgcg ccgggcctgg aacgcgatcg aacaagccga cgtggtgctc
     1201 tacctggtcg attcgagtcg cggcatgagc gacgcggatc gcaggataat cgccaccctg
     1261 ccccggaaca ggctgcaact ggtctacagc aagagcgatc tcctcgccac tgcgcccgcg
     1321 gacgaggccg gtgcgatcgc ggtgtcggtg agggacggtg ccggtaccga gcgcctgatc
     1381 gagcgaatca ccggccacac agatgatctc aaccaggata accagacatt catggcgcgc
     1441 cgccgccatg tcgaagccct gaagcgcgca gcgcaaagtc tcgatcaggc cacgacaacc
     1501 tttgccgcga gccgctcggg cgaactgatg gccgaagacc tgcgtgatgc acagcggcaa
     1561 ttgaacgaaa tcaccgggga attcaccagc gacgacttgc tggggcgaat cttcgcgagc
     1621 ttttgtatcg gcaaatgatg ccgtcgtgtt atgctggttc gaggaagcgc gctaagaact
     1681 gctgcagaat ctcgccatca tcgcggcaca tcagtaacag tcgccgatac tcgtcgggat
     1741 ccttgctgta ggtcagttct cccggtagcg ggaacaggcg gtcgtagtgt ctcgacaacc
     1801 agaaacgcag cgcagtgaaa cgcaacatgg ccgacaaagc cgagatctcg ggccggctga
     1861 gttcgcgctc gtgccgatag gcgccgagca atgcgccgcc gcgcgcttcg tcgagctctc
     1921 cgctcgcgcc gcgacaccag tcgtgcagta cgatggcgat gtcgagcacc aggctgtcat
     1981 ggcaggcgta atcgaaatcg cagataccgc atagcctttc accgtcgaat agcacattgt
     2041 cgtggaacag gtcggcgtgc accgcaccgg ccggcaagga ttcgaaatcc agtccccggt
     2101 actcgcgcag tgtcgaggca atcagcaacc ggtcggagtc gtcgagttcg gcatccagca
     2161 tgtcgctgac cgcgaacacc cagtcagcgc ctcgcgggtt tatgcggcgg gcggcgaaat
     2221 ccatgccggc ggaatgaaag cgtgccagct cggcgccgac cgccgcacag tgcgcgatgc
     2281 caggttgcgg ctgtacctcg ccgggcagtc gcgtgatgat cgcggccggg cgctggttaa
     2341 gcgtcgaaaa gaaatcgccg cgacggtcgc caaccggagc tgcgcaggct accgacctgg
     2401 cggcgaggtg gcgctgcagg ccgagcatgt agtagagctc gtcatcgctg tgatgctcgt
     2461 agagcgtgag cacgaatgcg ccgccctcgg tatcgacggt gtaattggta ttggtgatac
     2521 cggccgcgat cggagcgaag gcccgcagcc gaccgatatc gtagcgctcg agaaatgcct
     2581 cgagctgggg ttgcttgata ggggtaaaga cagacatcaa tcgttgcagc cggcgtcact
     2641 ggcgaattcg ccaacgcggc ggtaatatgc tccgggttca gggcctggca gtttaacgca
     2701 ttcgaatgaa tcaagagaac cagaaaaaac tcgtgctggt cgacggcagc tcttacctgt
     2761 ttcgggcgta tcacacgcgc ctcagccaga tgatgacgag cccgaccgga tttccgacac
     2821 aggcgatctt catcgtcgtc aatatgctgc gcaagctgct caaagacgaa cagcccgacc
     2881 gggtcgcggt ggtattcgat gccaagggca agacgtttcg caacgacatt tatcccgagt
     2941 acaaggccaa ccggccgccg atgcccgacg acctgcgcga acagatcgaa ccgctgcacg
     3001 aaatcattcg cgcgcagggc ttgccgctga tctgtgtcga aggcgtcgag gccgatgata
     3061 ttatcggcac gctgagccgc gaggcggtcg agcagggtta ccgggtattg atttccaccg
     3121 gagacaagga cctcgcgcag ctggtcaacg acaaggtcac gctgatcaat acgatgagca
     3181 acgaatcgct caacatcgat ggtgtacggg aaaagttcca ggtcggcgcc gagcagatcg
     3241 tcgattacct ggcgctgatg ggcgacagtt ccgacaacat tcccggcgtg cccaaggtcg
     3301 ggccgaaaac cgcggccaag tggatcgccg agttcggcag cctgcaaaat gtcatggatc
     3361 atgccgacca aatcggcggc aaggtcggcg agaacctgcg cgagtcgctg gatttcctgc
     3421 cgatgtccta ccaactggcg acggtaaaac tcgactgcga tacgggcacc acgatcgacg
     3481 agctcgaaca gcgcgacccc gatccggcgg tgctcgcggc cctgtaccgc gaatacggct
     3541 tcaaccgctg gctggaagaa ctcggcgccg ccagcgaaac ctcgcacggg caaaccgcgc
     3601 agcccgaggc gaattacgag tgcgtactga cccgcgaacg cttcgaccac tggctcgaac
     3661 gcctgcgtga ggccaacgga ttcgccgtcg ataccgagac cacgagcctc gactacatg
//
LOCUS       DATCZV010000128         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_644280, whole genome shotgun sequence.
ACCESSION   DATCZV010000128 DATCZV010000000
VERSION     DATCZV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644280"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /gene="lpxC"
                     /locus_tag="VIW27_02850"
     CDS             <1..487
                     /gene="lpxC"
                     /locus_tag="VIW27_02850"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY5738633.1"
                     /translation="VEFTPFNGFKVSFEIDFDHPLFTRQKQICHINFSTTSFVKEVSR
                     ARTFGFQKDIEYLRANNLALGGSQDNAIVLDDYRVLNEDGLRYDNEFVKHKILDSIGD
                     LYLLGHSLIGAFSGYKSGHALNNRLLRALLADEDAWEEVTFEDASTAPISYAQPVEAA
                     V"
     gene            495..1409
                     /locus_tag="VIW27_02855"
     CDS             495..1409
                     /locus_tag="VIW27_02855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5738634.1"
                     /translation="MNILFLSSNRGKICGLNLTKPLHLSLLLVSLALLLGAAGYAGYS
                     LNPGHDRQVLIEQWQTDVHRQRVKLENIQQEAEANIDALSSRVGLLQAHVMRLDALGR
                     KLVQMASIDKAEFDFDLTPAMGGPETVGNAGIESDELELAIDQLSLELESRQSQLAIL
                     EDLVLDENLQKEVQPSGRPITRGWISSYYGMRTHPLSGRREMHKGIDFAGKLGGEVIA
                     VAKGVVTYAGRRWGYGKVIDIAHGNGYTTRYAHNSRLLVSVGDTVEKGFQIAEIGSSG
                     RSTGPHVHFEVLKDGREINPVKFIRASN"
     gene            1499..>3118
                     /gene="secA"
                     /locus_tag="VIW27_02860"
     CDS             1499..>3118
                     /gene="secA"
                     /locus_tag="VIW27_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5738635.1"
                     /translation="MLSKVIKALFGSRNDRIVKKLSQRVARISLLEPQMQALDDAALR
                     QKTDEFKRRLSDGETLEQILDEAFAVCREASVRTLGLRHYDVQLIGGMILHNNQITEM
                     RTGEGKTLVATLPAYLNALSGKGVHIVTVNDYLAERDAEWMGAIYKFLGLSVGVVLST
                     MDYDTKRESYRADITYGTNNQFGFDYMHDNLSFTLEERVQRELNYAIVDEVDSILIDE
                     ARTPLIISGQASDSSDLYTRIDGIIPRLKRVETEDGPGDYSIDEKSKQAYLTEDGHDT
                     AEQLMVETGLLNEGESLYDAANIALVHHLNAGLRAHAIFQRDVDYIVQDGSIVIVDEF
                     TGRTMPGRRWSDGLHQAIEAKERVSIRHENQTIASITFQNYFRLYDKLAGMTGTADTE
                     AFEFQQIYGLEVVVVPTNKPMIREDLGDLIYLTKREKFDSIIDDIRDCVTRGQPVLVG
                     TTSVETSEYLSGLLDKVKIKHQILNAKQHEREARIVADAGIPGTVTIATNMAGRGTDI
                     VLGGNLEVDLKDIAEDQQQKRQQLAEEWQKLH"
BASE COUNT          690 a          928 c          914 g          586 t
ORIGIN      
        1 ggtcgagttc acgccgttca atggcttcaa ggtcagtttc gagatcgatt tcgatcatcc
       61 gctgttcacc cgccagaagc aaatctgcca tatcaatttt tcgactacct cgttcgtcaa
      121 ggaagtcagc cgtgcgcgca ccttcggttt tcagaaggat atcgagtacc tgcgcgccaa
      181 caacctcgcg ctcggcggta gccaggacaa cgccatcgtg ctggacgatt accgcgtgct
      241 gaacgaggac gggctgcgct acgacaatga attcgtcaag cacaagatcc tcgactcgat
      301 cggcgatctc tacctgctcg gccacagcct gatcggcgct ttttccggct acaagtcggg
      361 tcatgcgctg aacaatcgcc tgctgcgggc actgcttgcc gacgaagacg cctgggaaga
      421 agtgaccttc gaggatgctt cgaccgcgcc gatatcctac gcccaacccg tggaagccgc
      481 ggtataggtg aaccgtgaat atcctgttcc tgtcatccaa tcgaggcaag atatgcgggc
      541 tgaacctgac caagccgctg cacctgtcgt tgctgttggt gagcctggcg ctgttgctgg
      601 gtgccgccgg atatgccggt tacagcctga accccggcca tgacaggcag gtcctgatcg
      661 agcagtggca gaccgacgtg catcgtcagc gggtcaagct cgaaaacatt cagcaggaag
      721 ccgaagccaa catcgacgcg ctgtcgtcgc gcgtcggctt gctgcaggct catgtcatgc
      781 gtctcgacgc gctgggtcgc aagctggtgc agatggcaag catcgacaag gccgagttcg
      841 acttcgacct gacgccggcg atgggtggtc ccgaaacggt cggcaatgcg ggtatcgagt
      901 ccgacgagct ggagttggcc atcgaccagt tgagcctcga actcgaaagc cgccagagcc
      961 aactggcgat cctcgaggac ctggtgctcg acgagaatct gcaaaaagaa gttcagccca
     1021 gcggacggcc gatcacccgt ggctggatat cgtcttatta cggcatgcgt actcacccgc
     1081 tttcgggccg tcgcgagatg cacaagggta tcgattttgc cggcaagctc ggcggcgagg
     1141 tcatcgccgt agccaagggc gtggtgacct atgccggccg gcgctgggga tatggtaagg
     1201 tcatcgatat cgcgcatggt aacggttaca cgacgcgcta tgcgcacaac tcacgcttgc
     1261 tggtgtcggt cggtgatacc gtcgaaaaag gcttccagat cgccgaaatc ggttcctccg
     1321 gccgttccac gggacctcac gtgcatttcg aggtactgaa ggacggacgt gaaatcaatc
     1381 cggtaaaatt tatccgtgcc tcgaattaac cggtgacgcg tcttttttca cgcgacccgc
     1441 ttgaaagctt tcaattcggt ctccaacttc gctagatatt cgtcatagtg gtcgtacgat
     1501 gttgtccaaa gtcataaaag ccctgttcgg cagtcgtaac gaccgcatcg tcaagaaact
     1561 ctcgcagcgc gttgcgcgaa tcagcctgct ggagccgcaa atgcaggcgc tcgacgatgc
     1621 cgcgctgcgg caaaagaccg acgaattcaa gcgccgcctg agcgacggcg aaaccctgga
     1681 gcagatactg gacgaagcct tcgcggtctg ccgcgaggcc tcggtgcgca cgctcggcct
     1741 gcggcactac gacgtgcagc tgatcggcgg catgatcctg cacaacaacc agattaccga
     1801 gatgcgaacc ggcgagggca agacgctggt cgcgaccctg ccggcctacc tgaacgcact
     1861 gagcggcaag ggcgtgcata tcgtcaccgt caacgattat ctcgccgagc gcgacgcgga
     1921 gtggatgggg gcgatctaca agtttctcgg gctgagtgtt ggcgtggtgc tcagtaccat
     1981 ggattacgac accaagcgtg aatcctaccg cgccgacatt acctacggca ccaacaacca
     2041 gttcggcttc gattacatgc acgacaatct ttcctttacc ctcgaggaac gcgtgcagcg
     2101 cgagctgaac tacgccatcg tcgacgaggt cgactcgatc ctgatcgacg aggcgcgcac
     2161 gccgctaatc atctccggcc aggccagcga cagttcggat ctctacacgc gcatcgacgg
     2221 catcattccg cgactcaagc gggtcgagac cgaggatggt ccgggcgact acagcatcga
     2281 cgagaaatcg aaacaggctt acctgaccga ggacggtcac gacaccgcgg aacagttgat
     2341 ggtcgagacc ggcctgctca acgagggtga gagcctctac gacgcggcca atatcgcgct
     2401 cgtgcatcat ttgaatgccg gcctgcgcgc gcacgccatt tttcagcgcg acgtggacta
     2461 tatcgtgcag gacggttcga tcgtcatcgt cgacgaattc accggccgca cgatgcccgg
     2521 ccggcgctgg tcggatggcc tgcaccaggc aatcgaggcc aaggagaggg tcagcattcg
     2581 gcatgaaaac cagactatcg cgtcgattac cttccagaat tacttcaggc tttacgacaa
     2641 gctcgccgga atgaccggca ccgccgatac cgaggccttt gaattccagc aaatctatgg
     2701 cctggaagtg gtcgtggtgc cgaccaacaa gccgatgatc cgcgaagatc tcggcgatct
     2761 gatatacctc accaagcgcg agaaattcga ctcgatcatc gacgatattc gcgactgtgt
     2821 cacccgcggc cagccggtac tggtcggtac tacctcggta gagacgtcgg agtatctgtc
     2881 gggattgctc gacaaggtca agatcaagca ccagatactc aatgccaagc agcacgagcg
     2941 cgaagccagg atcgtcgccg acgcggggat accgggcacg gtgaccatcg ccaccaacat
     3001 ggccggacgc ggcacggaca tcgtgctcgg cggcaatctc gaggtagacc tgaaggatat
     3061 cgccgaagac cagcagcaga agcggcaaca gctggccgag gaatggcaga aacttcac
//
LOCUS       DATCZV010000129         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_749928, whole genome shotgun sequence.
ACCESSION   DATCZV010000129 DATCZV010000000
VERSION     DATCZV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749928"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VIW27_02865"
     CDS             complement(<1..362)
                     /locus_tag="VIW27_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738636.1"
                     /translation="MKQKLHKELEKLLEFFLEQRRNPPAEYAYGKGIVERPEIFTVKD
                     LQAHLNNPLLQPEWLEMFHGGKKISLEPAFKFKLIQRKELKFLDKTFINRLLADGAAA
                     VLEGLDILDQRINEFQARL"
     gene            complement(704..2167)
                     /gene="mltF"
                     /locus_tag="VIW27_02870"
     CDS             complement(704..2167)
                     /gene="mltF"
                     /locus_tag="VIW27_02870"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEY5738637.1"
                     /translation="MVELSPFAHRVQLVSGLVAAVVVLVFLTQSPGFERRDPNALERI
                     QQRGYLTVLTLNGATTYYQDINGPNGFEYQLASWFAQSIGVKPRFVTVSRFADIYPEL
                     VFGGGDIAAAGLSLGESEFSRSLAYGPRYYEVANQVLYRKSKSRRPREVSDLQGGSLK
                     IISGTAHIGLLRELQQQYPQLNWIEADDIAAEELIEQVDAGVVDYALADSHEIALQRR
                     YFPELRIAFELGEKRQLRWAYNHAEDDSLARAIERFFRTIEKDGRLTQLVHRHYSHVE
                     TFNYSDIRTFTSRVETHLPRYEALFKEAAEKVGFDWRLLASIGYQESLWNPRAKSPTG
                     VRGLMMLTRATAKQMKVKNRLDPAQSIRGGAEYIARVHKKVPQRIPEPDRTWLALAAY
                     NVGFGHVEDARIITQRRGGNPDRWIDVKENLPLLARKKWYENTRYGYARGWEPVKYVE
                     NIRQYFAYLADFDTRGAREVPGSGKPPREVAPLPPSL"
     gene            complement(2192..>2709)
                     /locus_tag="VIW27_02875"
     CDS             complement(2192..>2709)
                     /locus_tag="VIW27_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020581720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEY5738638.1"
                     /translation="DVYLGLDAYSLSPADVEFAQDHLRILSGLYGLLRPLDLMQPYRL
                     EMGTRLDTDQGSNLYQFWGDRITRSLNQELQQTGSATLINLASGEYFKSVKPSLLKAD
                     IVTPAFREFRDGEYKFIQFFAKKARGLMARYLVDQRIDEPEGLKDFDYEGYKYNASLS
                     TAKNWVFTRRQ"
BASE COUNT          493 a          804 c          845 g          567 t
ORIGIN      
        1 agcctggcct gaaactcgtt gatccgttga tcgagtatat ccagcccttc cagtaccgct
       61 gccgcgccgt cggcgagcag ccggttgatg aaggtcttgt cgagaaattt cagttccttg
      121 cgctgtatca gcttgaattt aaaggccggc tccagcgaga tctttttgcc gccgtgaaac
      181 atctccagcc actcgggttg cagcaacgga ttattgaggt gggcctgcag gtccttgacg
      241 gtgaatatct cgggacgctc gacgatgccc ttgccgtaag catactcggc gggcggattg
      301 cgccgttgct cgaggaagaa ttccagcagc ttttcgagct ccttgtgtaa cttctgtttc
      361 atgcgcttat ctcagtgttc agccgcttga tgacgagtcg tgatttgcgg ggttccaggg
      421 taaagcggta agaccggtga cgattttact cgactatgcc gggtttatgt agccgaaccc
      481 tgccagcagc ggcgcacggc tggaacgagc gtcggccgca tcgtcatcct gtctgtaccc
      541 ttcgctcgcg gttactcgcc agccaagcgt ctggtcttcg cccgcgcggc ggtgcgggca
      601 tggcgaccag cgatgcgcgg cacttgcggc agctatggtc ccatccgccg ccgctcacgg
      661 aaatcagggc ggttccggtc cgcaacgcgg attcgacccg attttacagg gacgggggga
      721 ggggggcgac ttcgcggggc ggtttgccgc tgcccgggac ctcgcgagca ccgcgggtgt
      781 cgaaatcggc caggtaggcg aagtactgac gaatattttc cacgtacttg accggttccc
      841 agccgcgcgc gtaaccgtag cgggtgttct cgtaccattt cttgcgcgcc agcagtggca
      901 ggttctcctt gacgtcgatc cagcggtcgg ggttgccgcc gcgccgctgg gtgatgatac
      961 gcgcatcctc gacgtgtccg aagccgacgt tgtaggcggc cagcgccagc caggtacggt
     1021 cgggctcggg aatccgttgc ggtactttct tgtgtacgcg ggcgatgtac tcggcgccgc
     1081 cgcgaatgct ttgcgccggg tcgagccggt tctttacttt catctgtttt gccgtggccc
     1141 gcgtcagcat catcaggccg cgcaccccgg tgggcgactt ggcgcgcgga ttccacagcg
     1201 attcctggta gccgatcgag gccagcaggc gccagtcgaa gccaaccttt tcagccgcct
     1261 ccttgaacag cgcttcgtat ctcggcaggt gggtctcgac gcgactcgta aaagtacgga
     1321 tgtccgagta attgaaggtc tcgacgtggc tgtaatggcg gtgcaccagc tgcgtcagcc
     1381 ggccgtcttt ctcgatggtc ctgaaaaacc gctcgatcgc tcgcgccagg ctgtcatcct
     1441 cggcgtggtt gtaggcccag cgcagctgcc gcttttcacc gagctcgaaa gcgatgcgca
     1501 actccggaaa atagcgccgc tgcagggcga tttcgtgcga atcggcgagc gcgtaatcga
     1561 ccacgccggc atcgacctgt tcgatcagtt cctcggccgc gatatcgtct gcctcgatcc
     1621 agttcaactg cgggtattgc tgctgcagtt cgcgcagcag gccgatgtgg gcggtgccgc
     1681 tgatgatctt cagcgagcca ccctgcagat cgcttacctc gcgaggacgc cgggatttcg
     1741 atttgcgata caacacctgg ttcgcgactt cgtaatagcg cggcccgtag gccagcgaac
     1801 ggctgaattc cgactcgccg agcgacagac cggcggcggc gatgtcaccg ccaccgaaca
     1861 ccagttcggg gtagatatcg gcaaagcggg acacggtgac gaagcgcggc ttgacgccga
     1921 tcgattgcgc gaaccagctt gccagctggt attcgaaacc attcggtccg ttgatgtcct
     1981 ggtaataggt ggtcgcgccg ttgagcgtca atacggtcag gtagccgcgc tgctgaatgc
     2041 gctcgagcgc gttcggatcg cggcgttcga accccggcga ctgggtcaga aacaccagta
     2101 cgacgacggc ggcaaccagg cccgatacca gttgcactcg atgggcaaat ggggagagtt
     2161 ctaccatcga tcagccgctg tcatacatcg gctactgcct gcgggtaaac acccaattct
     2221 tcgcggtcga cagcgacgcg ttgtatttgt agccctcgta atcgaagtcc ttcaaaccct
     2281 cgggctcgtc gatccgctgg tcgaccaggt agcgcgccat caggccgcgc gccttcttgg
     2341 cgaaaaactg gatgaacttg tattcaccgt cgcgaaattc gcgaaacgcg ggagtgacga
     2401 tgtcggcctt cagcagcgat ggcttgaccg atttgaaata ctcaccggag gccaggttga
     2461 tcagcgtcgc agagccggtc tgctgcaact cctggttcag cgagcgggtg atgcgatcgc
     2521 cccagaactg gtacaggttg ctgccctggt cggtatcgag gcgggtgccc atctcgagac
     2581 ggtagggctg catcaggtcg agcgggcgca acaggccata taatcccgag agtatgcgca
     2641 gatgatcctg ggcaaactcg acatccgcag gagacaggct gtaggcgtct agcccgagat
     2701 aaacatccc
//
LOCUS       DATCZV010000130         4071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_750007, whole genome shotgun sequence.
ACCESSION   DATCZV010000130 DATCZV010000000
VERSION     DATCZV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750007"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..563
                     /locus_tag="VIW27_02880"
     CDS             69..563
                     /locus_tag="VIW27_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY5738639.1"
                     /translation="MHVLVGITWIGLLYYFNFVQVPGVAKALAEANDGGPGPAAINKY
                     IAPTALWWFRWAALATWLTGAMALEAMHGGPGSGVVAAFTFAEGYRAIGMGAWLGTIM
                     MFNVWVLIWPNQKKILGLDGKEHPAEAIAKAKTVALMASRTNTLLSIPMLYGMTAQGH
                     GMLF"
     gene            681..1853
                     /locus_tag="VIW27_02885"
     CDS             681..1853
                     /locus_tag="VIW27_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5738640.1"
                     /translation="MTDHLVKAYQPLPVSFTHGDGCYLWDTEGKQYLDALCGISVTSL
                     GHNYPPLTEAICEQARTLLHTSNLYAIGWQQKLADRLCEVSQMERVFFANSGAEANEA
                     AIKLARLHGHQRGIDQPQILVMQHGFHGRTMATLSATGSRKVQAGFEPLVAGFVRAPY
                     DDIEAVEAIAANNRNLVAVLVEPVQGEAGIVIPKRGYLQALRRICDRHGWLLMLDEIQ
                     TGMARSGRLFAFQHENLLPDVVTLAKALGNGVPIGACLCRGPAASVFSPGKHGSTFGG
                     NPLACRAALTVLDSMIEHDIARNAAERGAELHALLEKGLAGNPRVREVRHLGLLLAVE
                     MQQACTQLPALALEQGLLINVTAERIVRLLPPLIIDAAQTAEIAARLIACINRLSD"
     gene            1858..2769
                     /gene="argF"
                     /locus_tag="VIW27_02890"
     CDS             1858..2769
                     /gene="argF"
                     /locus_tag="VIW27_02890"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY5738641.1"
                     /translation="MTKRHFLSLLDFSSDELRALIAHAIDVKQKLKSGISHTPLAGKL
                     LAMIFEKSSTRTRVSFEAGMYQLGGHAMFLSPRDSQLGRGEPIEDSARVISSMVDGIL
                     IRTFGHDRIVKLAEHSRVPVINGLSDLLHPCQLLADMQTWFELRGDIAGGKVAWIGDG
                     NNMCHSYINAAIRFDFNLAISCPAGHEPDAAILDSAAERVEICASAADAASDADLIVT
                     DVWASMGQEQEQEQRLQTFSAYQVNREVMARASRDALFMHCLPAHRGEEVTAEVLDSD
                     QSVVWQEAENRLHAQKALLEILLAPAA"
     gene            complement(2813..4012)
                     /locus_tag="VIW27_02895"
     CDS             complement(2813..4012)
                     /locus_tag="VIW27_02895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5738642.1"
                     /translation="MTIDDQSPPVNSHHLQQQELVAYLYHRFRFGIYITLLVSLLASA
                     LAWVELSIQGREHWVIGWYGLLCAILLVRWRVLQQFLGLANKHYFPYRLWHRRFFVGV
                     VATGLVLGCGAALLMPYITSNMQIVLHAMLLTMCAGGIAYLSTSFPVYISYMLTMMLP
                     VTLWLFLQHNTASYVLSCLYLFFMAAGWVSVRHMNRLVNDALYYRYDNEALIEDLQRL
                     LQSVSQTNKALEKISTTDEISGLANYRAFRVRLEEVWRQYRGNGARVSLIRLSPDHYH
                     EFNAHYGEQTGDRYLRELGALLSAQISNDNQLAARLQGTEFVLLLPGIGCANARQIAR
                     DIAAELERKKIAHGKSPAGLLTLSIGVGCQTVNADAESRELLVRADTALKLARERGGN
                     RVEVLEA"
BASE COUNT          781 a         1312 c         1245 g          733 t
ORIGIN      
        1 tggccatcgt gctgatattc attctcggaa ccaccggtac cgtaaatccg tggcagttct
       61 gggtctggat gcacgtactg gtcggtatca cctggatcgg actgctgtac tatttcaact
      121 tcgttcaggt tcccggcgtc gccaaggcac tggccgaggc caatgatggc ggtcccgggc
      181 cggcagcgat caacaagtac atcgcgccga ccgcgctgtg gtggttccgc tgggccgcac
      241 tggcaacctg gctgaccggc gcgatggcgc tcgaagccat gcacggcggg ccgggttcag
      301 gcgtggtcgc ggcgttcacc tttgccgagg gttatcgtgc tatcggtatg ggcgcgtggc
      361 tgggtaccat catgatgttc aacgtctggg tgctgatctg gcccaaccag aagaaaatcc
      421 tcggcctcga cggcaaggag cacccggcgg aggcgatcgc caaggcgaaa accgtcgcgc
      481 tgatggcatc gcgtaccaac acgctgctgt cgattccgat gctctacggc atgaccgcac
      541 agggacacgg tatgctgttc tagccgccgc ccgtccgcag ataaacaaaa aaccccgccg
      601 cggcggggtt ttttgtgcgc aaagcttgaa atcgccgaga atcggcgtat gtttgaccgt
      661 ttttttcacg cctccagccg atgaccgacc atctcgtcaa agcctaccag ccgctgccgg
      721 tcagttttac ccacggcgac ggctgttacc tgtgggacac cgagggtaaa caatacctcg
      781 atgcgctgtg cggcatctcg gttaccagcc tcgggcacaa ttatccgcca ctgaccgagg
      841 ccatctgcga gcaggcccgg accctgctgc acacctccaa cctgtacgcg atcggctggc
      901 agcaaaagct cgccgaccgc ctctgcgagg tgtcgcaaat ggagcgcgtg ttcttcgcca
      961 attccggcgc cgaggccaac gaggcggcga tcaagctggc gcggcttcac ggccaccagc
     1021 gcggcatcga tcaaccgcag atcctggtca tgcagcacgg ctttcatggc cgcaccatgg
     1081 cgaccctgag cgccaccggc agccgcaagg tgcaggccgg cttcgagccg ctggtcgccg
     1141 gcttcgtgcg cgccccttac gacgatatcg aggccgtgga ggcgatcgcg gccaacaacc
     1201 gcaacctggt tgcggtgctg gtcgagccgg tacagggcga ggcgggaatc gttatcccta
     1261 agcgcggcta cctgcaagcc ctgcggcgca tttgcgaccg gcacggctgg ctgttgatgc
     1321 tcgacgaaat ccagaccggc atggcacgca gcggccggct gttcgcgttt cagcatgaaa
     1381 acctgctgcc cgacgtggtg acgctcgcca aggcgctcgg caacggcgtg ccgatcggtg
     1441 cctgcctgtg ccgcggccct gcggcatccg ttttcagccc gggcaagcac ggctcgacct
     1501 tcggcggcaa cccgctcgcg tgccgcgcgg cgctgaccgt gctcgacagc atgatcgagc
     1561 acgatatcgc gcgcaatgct gccgagcgcg gcgctgaact gcatgcattg ctggaaaaag
     1621 gcctcgccgg caatccgcgg gtacgcgagg tacgccacct cggattgctg ctggcagtcg
     1681 aaatgcagca agcctgcacg caactgccgg cactggcgct ggagcaagga ttactgatca
     1741 acgtcaccgc ggagcgcatc gtgcgcctgc tgccgccgct aatcatcgat gcggcgcaaa
     1801 ccgccgaaat cgccgccagg ctgatcgcct gcattaaccg cctgagcgac tgaaaccatg
     1861 accaagcgcc atttcctgag cctgctggac ttttccagcg acgagttgcg tgccctgatc
     1921 gcgcatgcca tcgacgtcaa gcaaaagctg aagagcggca tttcccatac accgctggcc
     1981 ggcaagctgc tggcgatgat tttcgagaaa tcctcgaccc gcacgcgggt ttccttcgaa
     2041 gccggcatgt accagctcgg cgggcacgcg atgtttctgt cgccgcgcga cagccagctc
     2101 gggcgcggcg aaccgatcga ggacagcgcg cgagtcattt ccagcatggt cgacggcatc
     2161 ctgatacgca ccttcggcca cgatcgcatc gtcaagctgg cggagcattc gcgggtaccg
     2221 gtcatcaacg ggctcagcga cctgctgcac ccgtgccagt tactggccga catgcagacc
     2281 tggttcgaac tgcgcggcga tatcgccggc ggcaaggtgg catggatcgg tgacggcaac
     2341 aacatgtgcc attcgtatat caatgccgcg atccgcttcg atttcaatct cgccatttcc
     2401 tgccccgcgg gacacgagcc cgatgccgca atcctggatt ccgccgccga acgcgtcgaa
     2461 atctgcgcta gcgccgccga cgcggccagc gatgccgacc tgatcgtcac cgatgtctgg
     2521 gcgagcatgg gccaggagca ggaacaggag cagcgcctgc agaccttctc cgcttaccag
     2581 gtcaaccgcg aggtcatggc gcgggccagc cgcgacgccc tgttcatgca ctgcctgccg
     2641 gcacaccgcg gcgaggaagt caccgcggaa gtactggaca gcgaccaatc ggtcgtctgg
     2701 caggaagcgg aaaaccgcct gcacgcgcaa aaagctctgc tcgaaattct gctcgcgccg
     2761 gcagcctagt cggcgattcg ccattcgggc aggctgcggt gcgcttggcg ggctaagctt
     2821 cgagcacctc gacgcggttg ccgccgcgtt ctcgcgccag cttcagcgcg gtatcggccc
     2881 tgaccagcaa ttcgcgcgat tccgcatccg cgttcaccgt ctggcagccg acgccgatac
     2941 tcagggtcag caggccggct ggagatttgc cgtgcgctat tttcttccgc tccagctcgg
     3001 cggcgatatc gcgcgcaatc tgacgcgcgt tggcgcaacc gatgcccggc agcagcagca
     3061 cgaactcggt gccctgcagc cgcgctgcca gctggttgtc gttgctaatc tgcgcgctca
     3121 acagcgcgcc cagttcgcgc aggtaccgat cgccggtctg ctcaccgtag tgggcattga
     3181 attcatgata atggtcgggg ctgagcctga tcagcgagac gcgggcaccg ttgccgcggt
     3241 actgcctcca gacctcctcg agacgcaccc gaaacgcgcg gtaattggcg aggccgctga
     3301 tctcgtcggt agtcgatatc ttttccagcg ccttgttggt ttgcgaaacg gattgcagca
     3361 gccgctgcag gtcctcgatc agcgcctcgt tgtcgtagcg gtagtagagt gcgtcgttca
     3421 ccagccggtt catgtgcctg acgctgaccc agcccgccgc catgaagaac aggtacaggc
     3481 agctgagcac gtagctcgcg gtattatgct gcaggaacag ccacagcgtg accggcagca
     3541 tcatcgtcaa catgtaactg atatagaccg gaaacgaagt cgacaagtag gcgattccgc
     3601 cggcgcacat ggtcagcagc atggcgtgca gcacgatttg catattgctg gtgatatacg
     3661 gcattaacag ggccgcaccg cagccgagta ccagcccggt cgcgaccacg ccgacgaaga
     3721 aacgccggtg ccacagtcgg tacggaaaat agtgcttgtt ggccaggccg agaaactgct
     3781 gcagcacccg ccagcgcact aacaggatcg cgcacagcaa gccataccag ccgattaccc
     3841 agtgttcacg cccttgaatc gacaattcca cccaggccag cgccgaggcc agcagcgata
     3901 ccaacagggt gatgtaaata ccgaatcgaa atcgatggta gaggtaggca acgagttcct
     3961 gttgctgcaa atgatggctg ttgactggcg ggctctggtc gtcgatcgtc aactcccttc
     4021 acggcgtgga ttcaagcgct gcagcagccc ggcttgagct ggcagagctc g
//
LOCUS       DATCZV010000131         2765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_115055, whole genome shotgun sequence.
ACCESSION   DATCZV010000131 DATCZV010000000
VERSION     DATCZV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115055"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1477
                     /locus_tag="VIW27_02900"
     CDS             428..1477
                     /locus_tag="VIW27_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738643.1"
                     /translation="MQQPLRPLLNAVQRNCHISDARHAGNYSLCVYLLKMREYYRWEK
                     GYPFQHQLSPSDVGDWLKERESFWETIEDEPFAELPIDGKLYDPFDSAAVNTALRGYG
                     LVYSGGLGKHCTPHFFLGKLERRETQADWTVFLSQREYARDLTAPPAMALDGTIVVRR
                     ESLRRLIWEKIEEWRWNRLDNAMGRALRDLDLDGDIEAALDELTDTILDTVLLHEKGE
                     VLAGKRLGEEWEQMLASLPRSQAEIMARAVRDHLADALSTLPGLVADGEDAALHFYFA
                     NLANMHKHLFPALAQAYEDWLKQGRRTAIEALIPTSVSHWSALAERMLDIYRARPGDL
                     AARLQALVEDHRLQR"
     gene            1748..2407
                     /locus_tag="VIW27_02905"
     CDS             1748..2407
                     /locus_tag="VIW27_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5738644.1"
                     /translation="MGEVQVHALRGIDLELYQGEFVVLLGPSGSGKSTLLNILGGLDV
                     PTSGSVRYQDHDLSTFRESVLTRYRREHVGFVFQFYNLIPSLTARENIALVTEISVNP
                     LPPDEALAMVDLGDRADHFPAQLSGGEQQRVAIARAIAKRPDVLLCDEPTGALDIKTG
                     IVVLEALARVNRELGTTTVVITHNVAIAAMADRVIRLSDGRISEIEINSRKKSPSELN
                     W"
     gene            2412..>2765
                     /locus_tag="VIW27_02910"
     CDS             2412..>2765
                     /locus_tag="VIW27_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738645.1"
                     /translation="MRALDHKLWRDLWHLRGQCAAIVAVMASGVACYLMFMSTLDSLL
                     LSRDLYYAENRFADIFMPIKRAPEAVAGRLAAIDGVDKIDTRVAAPLTIDIDGFDEPV
                     VGNVTSIPDDGEPLLN"
BASE COUNT          527 a          928 c          837 g          473 t
ORIGIN      
        1 gtttgccctg gcgaaacagc atgccaccgg cgctgatccg cgtctcgatg gccgagtcgg
       61 cgaggaacct gatatgtcct tcctcgagcc cgcaaaaaaa cgggtgccgg gacaggtaat
      121 ccgcgatcga ctgactgttc atgctgcgcc gctccgcact tggtcgactc ccagactaaa
      181 cgggttgacg cgagattcct tgatccccgg caaaggcagg gcaaaaattt ttaccttgaa
      241 gcggtcgaat ccggcgccgg cggcattccc gccccacctc cccccgagct ggcgccgacg
      301 agatagtcat tcgggttgcg ccgccaatcc ccggctggcg gcatgccgcc accccgaatc
      361 gcgggtgaaa gaatcgcgca actagtgtaa tttccgtatt tcacgcaagc tttcacttcc
      421 tggattgatg caacaacccc tgcggcccct gctgaacgcg gttcagcgca attgccatat
      481 ttccgacgcg cgtcatgccg gcaattactc gctgtgcgtc tacctgctga agatgcgcga
      541 gtattatcgc tgggaaaagg gttacccgtt tcagcaccag ctttcgccga gcgacgtcgg
      601 cgactggctc aaggaacgcg agagcttctg ggaaaccatc gaggacgagc ccttcgccga
      661 gctgccaatc gacggcaaac tctacgaccc gttcgacagc gctgcggtaa acacggcgct
      721 gcgcggctac ggcctggttt acagcggcgg cctcggcaaa cactgcacac cgcatttctt
      781 cctcggcaaa ctggagcgtc gggagacaca ggcggactgg accgtgttcc tgtcgcagcg
      841 cgaatacgcc cgcgatctga ccgcgccgcc ggcgatggcg ctcgacggca ccattgtcgt
      901 gcgccgcgaa tcgctgcggc ggttgatctg ggaaaagatc gaggagtggc gctggaaccg
      961 gctcgacaac gccatgggac gcgcgctccg cgacctcgac ctggacggcg atatcgaggc
     1021 cgcgctcgac gaattgaccg acacgattct cgacacggtg ctgctgcacg agaaaggcga
     1081 ggtacttgcc ggcaagcggc tcggcgagga atgggagcaa atgctcgcca gcctgccgcg
     1141 ctcgcaggcg gaaatcatgg cacgcgcggt gcgcgatcac ctggccgacg cgctgtcgac
     1201 cctccccggc ctcgtcgccg acggcgaaga cgcggcactg cacttctatt tcgccaacct
     1261 cgcaaacatg cacaagcacc tgttcccggc gctggcgcaa gcctacgagg actggctaaa
     1321 gcagggtcgg cgcactgcga tagaggcgct gatcccgacc agcgtaagcc actggagtgc
     1381 gctggccgaa cgcatgctgg atatctatcg agcccgcccc ggcgatctcg ccgcaagact
     1441 gcaagccctg gtcgaagacc acagactgca gcgctaatcc ccgccacggc atgctcatga
     1501 gcggtccgcc gccttgcctt tgacggcccg gcgctgccga attcgccacc aggatgccct
     1561 ggccggggat ttccggcagg catcgcgccg ctttctgacc tgtatcatgt cttcgcggcg
     1621 cgatcgaatc tacatttccg ggtgcagtca acaactttag ggaaacaggt cagtgacagc
     1681 cgtcagtccg cgagccacca ccggggatgt tgttttccac acccgcggga taaccaaggt
     1741 ctaccagatg ggcgaagtgc aggtgcacgc gctgcgcggt atcgacctcg agctgtacca
     1801 gggcgaattc gtcgtgctgc tgggcccgtc gggcagcggc aaatcgacgc tgctgaacat
     1861 tctcggcggt ctcgacgtgc cgaccagcgg ctcggtgcgc taccaggatc acgatctcag
     1921 cactttcagg gaatcggtgc tgacccgtta ccggcgcgaa cacgtcggct tcgtgttcca
     1981 gttctacaac ctgatcccca gcctgaccgc gcgcgagaac atcgctctgg tcaccgagat
     2041 ctccgtgaac ccgctgccgc ccgacgaggc gctggcgatg gtcgacctcg gcgatcgggc
     2101 cgatcacttc ccggcgcagc tgtccggcgg cgagcagcag cgcgtggcca tcgcccgcgc
     2161 catcgccaag cgccccgacg tgctgctgtg cgacgaaccg accggcgcgc tcgacatcaa
     2221 gaccggcatc gtcgtgctcg aagcgctggc cagagtcaac cgggaactgg gcaccaccac
     2281 cgtggtaatc acccacaacg tcgccatcgc cgcgatggcc gaccgggtca tccgtctcag
     2341 cgacggccgc atctcggaga tcgagatcaa ctcgcgcaag aaatccccgt ccgagttgaa
     2401 ctggtaacgc aatgcgcgcc ctggatcaca agctgtggcg agacctctgg cacctgcgag
     2461 gccagtgcgc cgcgatcgtc gcggtgatgg ccagcggcgt cgcctgttac ctgatgttca
     2521 tgagtacgct ggattcgctg ctgctgagcc gcgacctgta ctacgccgaa aaccgcttcg
     2581 ccgatatctt catgccgatc aagcgcgccc ccgaggcggt cgccgggcga ctcgccgcga
     2641 tcgacggcgt cgacaagatc gatacccgcg tagcggcgcc gctgaccatc gatatcgacg
     2701 gcttcgacga accggtggtc ggcaacgtaa cctcgattcc ggacgacggc gagccgctgc
     2761 tcaac
//
LOCUS       DATCZV010000132         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_591440, whole genome shotgun sequence.
ACCESSION   DATCZV010000132 DATCZV010000000
VERSION     DATCZV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591440"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="hrcA"
                     /locus_tag="VIW27_02915"
     CDS             complement(<1..850)
                     /gene="hrcA"
                     /locus_tag="VIW27_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY5738646.1"
                     /translation="MSEKYELDERAQLLLRQLINSYTRDAQPVGSKNLARMSGLDVSP
                     ATIRNIMARLEDMGLVDSPHTSAGRVPTVAGYRFFIDSLLEVGDLAESAQQAIADIFS
                     PDKTSSDLIHSATDILSRVTHLAGIVSLTHTAPAEVRHIEFMKLSERRVLVILVINQD
                     DVHNKVIHVDRDYSDIELQQAARTLSGYLIGCNFETAREALLEELAELRTDANTIMQM
                     VLEAMDEVCTLNAAESLITSGETNLLEYAELSDINKLRNIFNVFNEKTDLLKLLDGCT
                     SAKGVEI"
     gene            980..1849
                     /locus_tag="VIW27_02920"
     CDS             980..1849
                     /locus_tag="VIW27_02920"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY5738647.1"
                     /translation="MALKFNSIGLVVRQDMQARHESIQRVIDVLERQATVRVHFLEFD
                     ANTREFSNIGLDQLVDAADLVISLGGDGTLLTAARALVDKDTPLLGINLGRLGFLADV
                     SFENFEPYLEAVVAGRYTVEERFFIQGRYYEGDELVSTRIALNDIILHSHESASMIEY
                     EISSGGDLIHVQRSDGVIVTTPTGSTAYALSGGGPIMHPSLDTLAIVPICPHTLSNRP
                     IVLPADQPIEVRLSQDSLLAKVSYDGHSTQMVKHDNRVLITRYPEPLSLLHPEDYDYF
                     KVLRAKLYWSTNY"
     gene            1849..3513
                     /gene="recN"
                     /locus_tag="VIW27_02925"
     CDS             1849..3513
                     /gene="recN"
                     /locus_tag="VIW27_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY5738648.1"
                     /translation="MLDSILIKDFAIIDAVQLELDQGMTALTGETGAGKSILLDALKL
                     AAGDRADSDSIRSGAERAEISVCFGLDPRHPASAWLQDNDMSADGECVLRRVLYANGR
                     SKAFINGSNATLMQLRALCDLLIDIHGQHEHQSLQKPAVQRELLDTYLGEAALISEVT
                     DKYLAYRALEKRLQQARSGAQQREQRIDLLELYCAELRELDLADGEFENLQQEYRRLS
                     NAGRLIEQSSEVLGLLYENEEHNLQSALAHCEQRLGELLASDPALASPHEMLGAALIQ
                     VQEASAELRGYLDGIELDAERLRTVDDRIARVQQLARKHRVEATQLAVLRGELENELA
                     DLRDDGQNIDTLEAELEKSREDYAFSAAELSLKRRQTAADLSEQITRVMQQLGMENGR
                     CLIEVADANEASAHGIDAIAYLVSANPGQPPKPLAKVASGGELSRISLAIQVIMSESS
                     HIPTLIFDEVDSGVGGAVAEIVGRKLRLLGEGRQVLCVTHLPQVASQAHHHFRVVKHS
                     LADDTTTAVIALDGDARREEIARMLGGVRVTEQSRAHASEMMASRD"
BASE COUNT          726 a         1101 c         1129 g          670 t
ORIGIN      
        1 agatttccac accctttgcc gaggtgcagc cgtcgagcag cttgagcagg tccgtttttt
       61 cattaaacac attaaaaatg ttgcgtaact tgttgatatc tgagagttca gcatactcaa
      121 gcaagttggt ttcgccactg gtaatcaggc tctccgcggc gttgagtgtg cacacctcgt
      181 ccatcgcctc gagcaccatc tgcatgatcg tattggcgtc ggtgcgtaat tcggcgagtt
      241 cctcgagcag cgcttcgcgc gcggtttcga aattgcagcc gatcaggtaa ccgctgaggg
      301 tgcgcgccgc ctgttgcaac tcgatatcgg aatagtcccg atcgacgtgt atcaccttgt
      361 tgtgcacgtc gtcctggttg atgaccagaa ttaccagcac gcgccgctcg ctcaatttca
      421 tgaactcgat gtggcgaact tcggccggcg cggtatgcgt caggctgacg atgcccgcca
      481 ggtgcgtgac ccgggagaga atatcggtgg cactgtgtat caggtcactg gaggtcttgt
      541 cagggctgaa aatatcggcg atcgcctgtt gcgcggactc ggccaggtcg ccgacctcga
      601 gcaggctgtc aatgaaaaag cggtagccgg cgacggtcgg cacacgcccg gccgaggtat
      661 gcggggaatc gaccagcccc atatcctcga gccgtgccat gatgttgcga atcgtcgccg
      721 ggctgacgtc gaggccggac attcgggcga gattcttgga gccgaccggc tgtgcgtcgc
      781 gggtatacga attgatcagc tggcgcaata aaagctgagc gcgttcgtca agttcgtatt
      841 tttcactcat cgggtctgat atattgttgg ccagcgacag tttagctgta atttgcccgc
      901 aggcaaacac aagctataga ctaactcccc gtaaatcaag ggattatagg ggccggaccg
      961 gagctcgaca gcaaatataa tggcgctgaa attcaattca atcggcctgg tcgtgcgcca
     1021 ggacatgcag gcccggcacg agagcattca gcgggtcatc gacgtgctcg agcggcaggc
     1081 gacggtgcgg gtgcattttc tcgagttcga tgccaatacc cgcgaatttt ccaacatcgg
     1141 gctggatcaa ctggtcgacg ctgccgacct ggtgatttcg ctgggcggcg acggtaccct
     1201 gctgacagcc gcccgcgcgc tggtcgacaa ggatacgccg ttgctgggca tcaacctcgg
     1261 gcgcctgggc tttcttgccg acgtgtcgtt cgaaaatttc gaaccctacc tggaagcggt
     1321 ggttgccggc cgttatacgg tcgaggagcg gtttttcatc cagggccgct actacgaggg
     1381 cgacgagctg gtatcgaccc gcatcgcgct caacgacatc attttgcaca gccacgaatc
     1441 ggcgagcatg atcgagtacg agatctccag cggcggcgac ctgattcacg tacagcgttc
     1501 ggatggcgtg atcgtcacca cgcccaccgg ttccacggcc tacgcgcttt ccggcggcgg
     1561 gccgatcatg catccgtcgc tggacacgct ggcgatcgtg ccgatctgcc cgcacacgct
     1621 tagcaatcgg ccgatcgtac tgccggccga ccagccgatc gaggtccgcc tgagccagga
     1681 ttcgctgctc gccaaggtca gctacgacgg tcattcgacg caaatggtca agcacgacaa
     1741 cagagtgctg atcacgcgct acccggaacc gctgtcgctg ctgcaccccg aggactacga
     1801 ttacttcaag gtgctgcgcg ccaagctcta ctggagtacc aactactagt gctcgactcg
     1861 atactgatca aggatttcgc gatcatcgac gcggtgcagc tcgagctcga ccagggcatg
     1921 accgcgttga ccggcgagac cggtgccggt aaatcgatcc tgctcgatgc gctcaagctc
     1981 gccgctggcg accgtgccga cagcgacagc attcgcagtg gcgcggaacg cgccgaaatc
     2041 tcggtttgct ttggcctcga tccgcgtcac cccgcatccg catggctgca ggacaacgac
     2101 atgtccgccg acggtgagtg cgtgctgcgg cgcgtgctct acgccaacgg acgcagcaag
     2161 gcgttcatca acggcagcaa tgcgacgctg atgcaattac gcgcgttgtg cgacctgttg
     2221 atcgatattc acggacagca cgagcaccag tcgctgcaaa agccggccgt gcaacgcgaa
     2281 ctgctggata cctatctcgg cgaagccgcg ctgattagcg aggtaaccga caaatacctg
     2341 gcctaccggg cgctggaaaa acgcctgcag caggcgcgct cgggagcgca gcagcgcgaa
     2401 cagcgaatcg acctgctcga gctgtattgc gcagaactgc gggaactcga cctggccgat
     2461 ggcgagttcg agaacctgca acaggagtat cgccggctgt cgaatgccgg tcgactgatc
     2521 gagcagagca gcgaagtgct cgggctgttg tacgaaaacg aggaacataa cctgcagtcg
     2581 gcgctggcgc attgcgaaca gcgcctcggt gagctgctgg ccagcgaccc ggcactggcc
     2641 agcccccacg aaatgctcgg cgcggccctg atccaggtgc aggaagccag tgccgagcta
     2701 cgtggttacc tcgacggcat cgagctcgac gcggagcggt tgcgcaccgt cgacgatcgc
     2761 atcgcccgcg tccagcagct ggcgcgcaag caccgcgtcg aagcgacgca actggcggtg
     2821 ttgcgcggcg agctggaaaa cgagctcgcc gacctgcggg acgacgggca aaatatcgac
     2881 acgctcgagg ccgagctcga aaaatcacgc gaggactacg cattcagcgc cgccgaactg
     2941 tcgctgaagc gccgccagac cgcggccgac ctgtcggagc agatcacccg cgtgatgcag
     3001 caactgggaa tggagaacgg ccgatgtctg atcgaggtgg ccgacgcgaa cgaagcctct
     3061 gcccatggca tcgacgccat cgcctatctg gtcagcgcca atccgggcca gccgccgaag
     3121 ccgcttgcca aggtggcctc gggtggcgaa ctgtcacgca tcagcctcgc gatccaggtg
     3181 atcatgagcg aatcgtcgca tattccgacg ctgatattcg acgaggtcga cagcggcgtc
     3241 ggcggcgcag tcgccgagat cgtcggtcgc aagctgcgcc tgctgggaga gggccgccag
     3301 gtgctgtgcg tgactcacct gccccaggtc gcgtcccagg cccatcacca ttttcgagtc
     3361 gtcaagcaca gcctggcgga cgacaccacg accgcggtga tcgcgctcga cggcgacgcg
     3421 cgccgcgaag agattgcccg tatgctcggc ggggttcgcg tcacggaaca gtcacgcgct
     3481 cacgccagtg agatgatggc gagccgggat taaaccgcgc agcgcgcaaa ccgctcgagc
     3541 ggttttcggc gcagcccatg gcaggcgctt attgggagga ggcgcggtct cggctaccgg
     3601 ccggggttac ttttccttga agtggc
//
LOCUS       DATCZV010000133         2347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_273736, whole genome shotgun sequence.
ACCESSION   DATCZV010000133 DATCZV010000000
VERSION     DATCZV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273736"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VIW27_02930"
     CDS             complement(<1..1078)
                     /locus_tag="VIW27_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY5738649.1"
                     /translation="MEYRDLRDFIDRLERDGDLKRISQRVDPQLEITEIANRVLRAGG
                     PALLFEQPGNSGIPLLANLFGTEQRVAQALGEESATSLREVGKLLAFLKEPAPPKGFK
                     EAMKTLPIYKKVLDMSPRQVKKAPCQEVQLHGEEVDLSMLPVQTCWPGDAGPLITWGL
                     VTTRGPAMQRQNLGIYRQQVIGRNRVIMRWLSHRGGALDYREWCRQNPDQPFPLSVVL
                     GCDPATILAAVTPIPDSLAEYAFAGLLRGSKTEVTRSLLSDLVIPARAEIVLEGRINP
                     GDEADEGPFGDHTGYYNEVERFPVFTVEAMSMRKNPIYHSTYTGRPPDEPSVLGLALN
                     EVFIPILQKQYPEIIDFYLPAEGCS"
     gene            complement(1079..1738)
                     /locus_tag="VIW27_02935"
     CDS             complement(1079..1738)
                     /locus_tag="VIW27_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEY5738650.1"
                     /translation="MLKRTFLGLALMAYCVILSAQDTSGDRHYVTDELRLSMYSNAND
                     NSQVLKLLQSGDSVDIEQIQGPYALVLGPDGTRGWVKRGFLVTKPTSNLLLAEEKQKT
                     AQLAAEIEKLANSKTVIEQYEQDMDAMAARLAGVEDEKQQAQASIAALEQQIEAQQRE
                     LDARYEGGYAAWLALWDTARHYWLYLLPIVALIVLLTSLISKSIVESRIKARFQGIKI
                     W"
     gene            complement(1742..>2347)
                     /locus_tag="VIW27_02940"
     CDS             complement(1742..>2347)
                     /locus_tag="VIW27_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY5738651.1"
                     /translation="MLTRIDVPDVAGINGVEWDAVELPSQFLENFCWQREALALFARH
                     YRTDEPLPDDLYQRMLESKNFQSALQMLRQIEFALFDIRLHQADIESADQVQALLDEV
                     RARVSVVATPPINRFQCGFSHIFAGGYAAGYYSYKWAEVLSADAFAAFEEEGIFNPET
                     GRRFLYCVLEQGGSRPAMESFRCFRGREPQIEALLRHSGIS"
BASE COUNT          447 a          709 c          694 g          497 t
ORIGIN      
        1 aggagcaacc ctctgccggc aggtagaaat cgatgatttc ggggtactgc ttttgcagta
       61 tcggaataaa cacctcgttc aacgccagcc ccagcaccga cggctcgtcg ggcgggcggc
      121 cggtgtaggt gctgtggtag atggggttct tgcgcatgct catcgcctcg accgtgaata
      181 ccggaaagcg ctcgacctcg ttgtagtagc cggtatggtc gccgaacggc ccctcgtcgg
      241 cctcatcgcc ggggttaatg cgaccttcga gcacgatctc ggcacgcgcc ggtatcacca
      301 ggtccgagag cagcgaacgg gtgacctcgg ttttcgagcc gcgcagcaag cctgcgaagg
      361 cgtactcggc cagcgaatcg ggtatcggcg tgaccgcggc gagaatggtc gccggatcgc
      421 aaccgagcac caccgacagc ggaaacggct ggtccgggtt ttgccggcac cactcgcggt
      481 aatccagcgc gccgccgcga tgcgacaacc agcgcatgat cacgcggttg cggccgatga
      541 cctgctgccg gtaaataccc aggttttgcc gctgcatcgc aggtccccgg gtggtgacta
      601 gcccccaggt gatcaatggg ccggcgtcgc cgggccagca ggtttgtacc ggcagcatcg
      661 acaggtcgac ctcctcacca tgcagttgaa cttcctggca cggggctttt ttgacctgcc
      721 tgggcgacat atcgaggact ttcttgtaga tcggcagggt tttcatcgct tccttgaatc
      781 cctttggcgg tgccggctcc ttgaggaatg caagcagttt gccgacctcg cgcagcgagg
      841 tcgccgattc ttcgccaagc gcctgggcga cgcgctgctc ggtgccgaac aggttcgcga
      901 gtagcggtat gccgctgttt ccgggttgct cgaacaacag cgccgggccg ccggcccgca
      961 gcacgcggtt ggcgatctcg gtaatttcca gttgtggatc gacgcgctgg ctgatgcgct
     1021 tcaggtcacc gtctcgctcc agccggtcga tgaaatccct tagatcacgg tattccattt
     1081 accagatctt gatgccttga aagcgggcct tgatgcggga ctcgacgatg gacttactga
     1141 tcaacgaggt cagcaggacg attagcgcga ctatcggcag caagtagagc cagtaatgcc
     1201 tggccgtgtc ccacagcgca agccacgccg cgtaaccgcc ctcgtagcga gcgtcgagtt
     1261 cgcgctgctg ggcctcgatc tgttgctcga gcgcagcaat gctcgcctgt gcctgctgtt
     1321 tctcgtcttc gacaccggca agccgggcgg ccatcgcatc catgtcttgc tcgtattgct
     1381 cgatcaccgt cttgctgttg gcgagctttt cgatctcggc ggcgagttgt gcggtttttt
     1441 gcttttcctc ggcgagcagc aggttggagg tcggcttggt aaccagaaat ccccgcttga
     1501 cccagccccg cgtaccatcg ggaccaagca ccagtgcata gggtccctgg atttgctcga
     1561 tatcgaccga gtcgcctgac tgcagtaatt tcaacacctg gctgttgtcg ttggcgttcg
     1621 aatacatcga caaccgcagc tcgtcggtga cgtaatgtcg gtcgccgctg gtgtcctgcg
     1681 cagacaaaat tacgcagtac gccatcagcg cgaggcccag aaacgttcgc tttaacatcg
     1741 atcagctaat tccactatgc ctcagcaggg cttcgatttg cggctcgcga ccgcgaaagc
     1801 agcggaacga ttccatcgcg ggccttgaac cgccctgctc gagtacgcaa tacaaaaatc
     1861 tccgcccggt ctcgggattg aaaatacctt cttcttcaaa tgctgcaaat gcgtcggcgg
     1921 aaagaacctc cgcccacttg tagctataat aacccgcggc atatccacct gcaaaaatat
     1981 gtgaaaaacc acactgaaat cggttgatcg gcggtgtcgc taccaccgat acgcgggcgc
     2041 gcacctcgtc gagcagggcc tgtacctggt cggccgattc gatgtcggcc tggtgcaggc
     2101 gaatatcaaa cagcgcgaac tcgatttgcc tgagcatttg cagcgcgctt tgaaaatttt
     2161 tcgattccag catgcgctgg taaaggtcgt ccggcagcgg ctcgtcggtg cggtagtggc
     2221 gtgcgaacaa tgccagcgct tcgcgctgcc agcagaaatt ttccaggaac tggctcggca
     2281 gttcgaccgc gtcccattcg acgccattga taccggcaac atcggggacg tcgattcgag
     2341 tcagcat
//
LOCUS       DATCZV010000134         6818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_168417, whole genome shotgun sequence.
ACCESSION   DATCZV010000134 DATCZV010000000
VERSION     DATCZV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168417"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /gene="acnA"
                     /locus_tag="VIW27_02945"
     CDS             complement(<1..1824)
                     /gene="acnA"
                     /locus_tag="VIW27_02945"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5738652.1"
                     /translation="MNEQYRKPLPGTGLDYYDTRAAVEAIDADAWDRLPYTARVLAEN
                     LVRRCDPAALDASLRQIVERRRELDFPWYPARVVCHDILGQTALVDLAGLRDAIAHQG
                     GDPAQVNPVVPTQLIVDHSLAVEYAGFDPEAFAKNRAIEDRRNADRFHFIEWCKTAFG
                     NVDVIPAGNGIMHQINLEKMSPVIQARDGVAFPDTCVGTDSHTPHIDCLGVIAIGVGG
                     LEAENVMLGRASMMRLPEIVGVRLAGRRQPGITATDIVLALTEFLREARVVGAWLEFF
                     GDGAASLTIGDRATISNMAPEYGATAAMFHIDEQTLEYLRLTGREAEQVALVENYAKT
                     AGLWADDLREAEYERVLEFDLSTVERNMAGPSNPHRRLPTSALGERGIAAPWEQNPGE
                     MPDGAVIIAAITSCTNTSNPRNVVAAGLIARKANQLGLLRKPWVKSSFAPGSRVAKLY
                     LEEAGLLAELEKLGFGIVAYACTTCNGMSGALDPAIQQEIIDRDLYATAVLSGNRNFD
                     GRIHPYAKQAFLASPPLVVAYAIAGTVRFDIEKDALGEDVDGKPIRLRDIWPDDAEID
                     AIVAEHVRPEQFRQVYTPMFDLGEIEPAESPLYDWRPQSTYI"
     gene            complement(2102..3250)
                     /gene="prpC"
                     /locus_tag="VIW27_02950"
     CDS             complement(2102..3250)
                     /gene="prpC"
                     /locus_tag="VIW27_02950"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY5738653.1"
                     /translation="MNDTPGFKPKKSVALSGTAAGNTAICTVGRTGNDLHYRGYDILD
                     IAETCEFEEIAYLIVHEKLPTRDELAGYKARLKAMRGLPHEVKTVLEQLPSSTHPMDV
                     MRTGCSALGCLEPEAAISDYEATRTIIDRLMACLGSMLAYWYHYSHHGKRIELVTDDD
                     SIGGHFLHLLHGEPPRESWVKAMHISLILYAEHEFNASTFTGRVVAGTNSDIYSCITA
                     AIGALRGPKHGGANEEAFRIQSRYASADEAEADIRERVANKEIVIGFGHPVYTVSDPR
                     NEVIKRLAKSLSEENGDMLMFDIAERLESVMWELKNMFPNLDWFSAVSYNQMGVPTDM
                     FTPLFVISRTSGWGAHIVEQRQDGKIIRPSANYIGPEDLEYVPIDERS"
     gene            complement(3247..4134)
                     /gene="prpB"
                     /locus_tag="VIW27_02955"
     CDS             complement(3247..4134)
                     /gene="prpB"
                     /locus_tag="VIW27_02955"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY5738654.1"
                     /translation="MTDSNTPGNKLRDAVAANNPLQVVGAVNAYFAIMAEKSGHQALY
                     LSGGGVAACSLGTPDLGISTLADVLEDARRITDASTLPLLVDVDVGWGGAFNIARCIR
                     EMTRAGVAGVHIEDQIMQKRCGHRPNKAIVSSAEMVDRVKAAVDARSDDSLVIMARTD
                     ALAVEGMEAAIDRAAACVEAGADMIFPEAMTELDQYRRFVDAVRVPVLANITEFGATP
                     LFTTDELASAGVKLALYPLSAFRAASKAALVVYQQLLADGTQQSVVDSMQTRMELYDF
                     LDYHEYEQKLDQLFSEETS"
     gene            complement(4127..4825)
                     /locus_tag="VIW27_02960"
     CDS             complement(4127..4825)
                     /locus_tag="VIW27_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5738655.1"
                     /translation="MTEIESSLVEQGILESPTLAGRTFDSIKADILSGELAQGSKIVE
                     ADLALKYGISRGPLREAIHRLEQLKLIVRIPHAGSRVVTLDVKMMEDIYVARESLEGM
                     AARLAARLMSDADIAALNDLLDQHEAAIREVDGKAYFQREGDIDFHYRIATASGNRWI
                     LENLNGELYQLIRMCRRQSGQVPERAPIALAQHRQIAAAITARDEELAEILMRRHISG
                     AWKIVKRILEEQHD"
     gene            5153..6457
                     /locus_tag="VIW27_02965"
     CDS             5153..6457
                     /locus_tag="VIW27_02965"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY5738656.1"
                     /translation="MSDETVTLIDDTTGKEIKLPLYTPTAGPKVIDIGKLYKELGYFT
                     YDPGFMSTASCSSAITFLDGGKGVLQYRGYPIEQLAEHSSYLEVCYLLLNNRLPTKSE
                     LDHFENDVTRHTMLHEAMIRFFTGFRRDAHPMAIMVGCVGALAAFYHDHMDIHDAEHR
                     MITAYRLVAKMPTIAAMSYRYANGKPFVYPRNALSYTENFLYMMFSMPSEDYTPDPLL
                     AKALDLIMILHADHEQNASTSTVRLAGSSDANPFAAVASGIAALWGPAHGGANEAVIR
                     MLVDINESGKPLRDFVNRAKDKNDPFRLMGFGHRVYKNYDPRAKLIREICHKLLNDME
                     TSGEQKKLLETAMALEKVALEDDYFIERNLYPNVDFYSGIIFLAMGIPMNMFTVIFAM
                     ARTIGWISQWNEMMEDEDAKIGRPRQLYNGAIDLEYLPIDKR"
     gene            6506..>6818
                     /locus_tag="VIW27_02970"
     CDS             6506..>6818
                     /locus_tag="VIW27_02970"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020284711.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HEY5738657.1"
                     /translation="MLDAYREHVEARAAEGVPPKPLDAQQTAELVDLLKNPPAGEEQF
                     LVELLTRQVPAGVDEAAYVKAGFLAAITRGEAQSPLIDRVRAVEILGTMLGGYNIEPL
                     IA"
BASE COUNT         1216 a         2094 c         2174 g         1334 t
ORIGIN      
        1 aatgtaggtg gattgcggcc gccagtcgta aagcgggctc tccgccggct cgatttcgcc
       61 gagatcgaac atcggcgtgt aaacctggcg gaactgctcc ggcctgacgt gctcggcgac
      121 gatggcgtcg atctcggcgt cgtccggcca gatgtcgcgc agtcgaatcg gcttgccgtc
      181 gacgtcctcg ccgagcgcat ccttttcgat atcgaagcgc accgtgccgg cgatcgcgta
      241 agcgactacc agcggcggcg aggcgaggaa agcctgcttg gcgtagggat ggatgcggcc
      301 gtcgaagttg cggttacccg acagcaccgc ggtcgcgtac aggtcgcggt cgatgatttc
      361 ctgctggatc gccgggtcca gcgcgccgct catgccgttg caggtggtgc aggcataggc
      421 gacgatgccg aagccgagct tttccagctc ggccagcagg ccggcctcct cgaggtacag
      481 cttcgcgacc cgcgagccgg gggcgaacga cgatttcacc cacggcttgc gcagcaggcc
      541 gagctggttg gccttgcgcg cgataaggcc ggcggcgacc acgttgcgcg ggttgctggt
      601 attggtacag ctggtgatcg cggcgatgat caccgcaccg tccggcatct cgccgggatt
      661 ctgttcccag ggcgcggcga tgccgcgctc gccgagtgcg ctggtcggca ggcggcgatg
      721 cgggttggat gggccggcca tgttgcgctc caccgtcgac aggtcgaatt cgagcacccg
      781 ttcgtactcg gcttcgcgca aatcgtcggc ccacaggccc gcggttttcg catagttttc
      841 gaccagcgcc acctgctcgg cttcgcgacc ggtcagcctc aggtattcga gggtctgctc
      901 gtcgatatgg aacatcgccg cggtggcgcc gtactcgggg gccatgttgg agatcgtcgc
      961 gcggtcgccg atggtgagac tggccgcgcc gtcgccgaag aattccagcc aggcgccgac
     1021 cacgcgcgct tcgcgcagga actcggtcag cgccagcacg atatcggtgg cggtgatgcc
     1081 cggctggcgc ctgcccgcca gacgcacgcc gacgatctcc ggcaggcgca tcatcgacgc
     1141 gcggccgagc atgacgttct cggcttcgag tccgccgacg ccgatcgcga ttacgccgag
     1201 gcagtcgata tgcggcgtgt ggctgtcggt gccgacgcag gtgtcgggaa aggcgacgcc
     1261 gtcgcgcgcc tggatcaccg gcgacatctt ttccaggttg atctggtgca tgatgccgtt
     1321 gcctgccggg atcacgtcga cgttgccgaa cgcggtcttg caccactcga tgaaatgaaa
     1381 gcgatcggca ttgcgccggt cctcgatcgc gcggttcttg gcgaaagctt cggggtcgaa
     1441 accggcgtac tcgacggcca gcgagtggtc gacgattagc tgcgtcggca ccaccgggtt
     1501 gacctgcgcc ggatcgccgc cctgatgggc gatcgcgtcg cgcagcccgg cgagatcgac
     1561 cagcgcggtc tggccgagaa tatcgtgaca gacgacgcgt gccgggtacc acggaaagtc
     1621 gagttcacgc cggcgctcga cgatttgccg caacgaggcg tcgagcgccg ccgggtcgca
     1681 acgccgcacc aggttctcgg cgagcacgcg cgcggtgtac ggcaggcgat cccaggcatc
     1741 cgcgtcgata gcctcgacag cggcgcgggt gtcgtaatag tcgagcccgg taccgggcag
     1801 gggtttgcga tattgctcgt tcatctcttt acctgtcatc ccctgtatgc ctgaccgcgc
     1861 gagtggcgca atcggcccgg tgggggcttt tgcctgtggc tgccagtatt cggtaactca
     1921 actggcgagc cttcgatcgg gccggtcaac ggaaatggat ttcgggaccg ctgccagcac
     1981 atccctgtgc gcttgcggcg cgacatccgt gtcgcttagc atcccgcaat ccatttccgc
     2041 cgaccgaccc tccaattttt ccaaccggac tcactactcc gcctgtaagg accggcgtta
     2101 actatgagcg ttcgtcgatc ggcacgtact cgaggtcttc cggaccgatg taattggccg
     2161 acgggcgaat gatcttgccg tcctggcgct gctcgacgat gtgcgcgccc cagcccgagg
     2221 tgcgcgaaat cacgaacagc ggcgtgaaca tatcggtcgg cacgcccatc tggttgtagg
     2281 agaccgccga aaaccagtcg aggttgggga acatgttctt cagctcccac atcaccgact
     2341 cgagtcgctc ggcgatgtcg aacatcagca tatccccgtt ctcctcggac aggcttttcg
     2401 ccagccgctt gatgacttcg ttgcgcggat cgctgaccgt gtagaccggg tgaccgaaac
     2461 cgatgacgat ttccttgttg gcgacgcgct cgcgaatgtc ggcctcggcc tcgtcggcgg
     2521 aagcgtaacg cgactggatg cgaaacgcct cctcgttggc gccgccgtgc ttgggaccgc
     2581 gcagtgcgcc gatcgccgcg gtgatgcacg agtagatgtc ggaattggtg ccggcgacca
     2641 cgcggccggt gaaggtcgag gcattgaact cgtgttcggc gtagaggatc agcgagatgt
     2701 gcatcgcttt cacccaggat tcgcgcggcg gttcgccgtg cagcaggtgc aggaaatgac
     2761 cgccgatcga atcgtcgtcg gtgaccagtt cgatgcgctt gccgtggtgg ctgtagtgat
     2821 accagtaggc cagcatcgaa ccgaggcagg ccatcaggcg gtcgatgatc gtgcgggtcg
     2881 cctcgtaatc gctgatcgca gcctcgggct ccaggcagcc gagcgccgag cagccggtgc
     2941 gcatcacgtc catcgggtgg gtgctcgacg gcagctgttc gagtacggtc ttgacctcgt
     3001 gcggcaggcc gcgcattgcc ttcaggcgcg ccttgtagcc ggcgagctcg tcgcgggtag
     3061 gcagcttttc gtgcacgatc aggtaggcga tttcctcgaa ctcgcaggtt tcggcgatat
     3121 cgagtatgtc gtagccgcgg taatgcagat cgttgccggt acggccgacg gtacagatcg
     3181 cggtgttgcc ggccgcggtg ccggatagtg cgactgattt cttgggtttg aatccgggtg
     3241 tgtcgttcat gatgtctcct cgctgaataa ctggtccagc ttttgttcgt attcgtggta
     3301 atcgagaaag tcgtacagct ccatgcgcgt ctgcatgctg tcgacgacgc tttgctgcgt
     3361 gccgtcggct agcagctgct ggtagacgac gagcgcggcc ttgctcgcgg cgcgaaacgc
     3421 cgacagcggg tagagcgcca gcttgacccc ggccgaggcc agttcgtcgg tggtgaacag
     3481 cggcgtggcg ccgaactcgg tgatattggc cagtaccggc acgcgcaccg cgtcgacgaa
     3541 gcggcggtac tgatcgagtt cggtcatcgc ctcgggaaag atcatgtcgg cgccggcctc
     3601 gacgcaggcc gcggcgcgat cgatcgctgc ctccatgccc tcgaccgcga gcgcgtcggt
     3661 gcgcgccatg atcaccaggc tgtcgtcact gcgcgcatcg acggcggcct tgacgcggtc
     3721 aaccatttcg gcgctcgaga cgatcgcctt gttggggcgg tgaccgcagc gcttttgcat
     3781 gatctggtcc tcgatatgca cgccggcgac gccggcgcgg gtcatttcgc gaatgcagcg
     3841 cgcgatgttg aacgcaccac cccagccgac gtcgacgtcg accagcagcg gcagggtgct
     3901 cgcatcggtg atgcggcgcg cgtcctcgag cacgtcggcg agcgtgctga tgccgaggtc
     3961 gggtgtgccc agcgagcagg cggcgacgcc gccgcccgac aggtagagtg cctggtggcc
     4021 gcttttttcg gccatgatcg cgaagtaagc gttgaccgcg ccgacgacct gcagcggatt
     4081 gttggccgcc accgcgtcac gcagcttgtt gccgggagtg ttcgaatcag tcatgttgtt
     4141 cctcgagtat gcgtttgacg attttccagg cgccgctgat gtgccggcgc atgaggattt
     4201 cggcgagctc ttcgtcgcgc gccgtgatcg cggccgcgat ctggcgatgc tgggcgagcg
     4261 cgatgggcgc gcgttccggc acctgtcccg actgccgccg gcacatgcgg atcaactggt
     4321 agagttcgcc gttcagattt tcgagtatcc agcggttgcc actggcggtc gcgattcgat
     4381 aatgaaaatc gatgtcgccc tcgcgctgga aataagcctt gccgtcgacc tcgcgaatcg
     4441 ccgcttcgtg ctggtcgagc aagtcgttga gcgcggcgat atcggcgtcg ctcatcagcc
     4501 gtgccgcgag ccgcgcggcc atgccctcga gcgattcgcg ggccacgtag atgtcttcca
     4561 tcattttcac gtcgagagtg acgacgcgcg agccggcatg cggaatacgc acgatcagct
     4621 tcaactgttc gagccgatga atggcttcgc gcaacggtcc gcggctgatg ccgtacttca
     4681 gcgccaggtc cgcttcgacg atcttgctac cctgagcgag ttcgccgctg aggatgtcgg
     4741 ccttgatgct gtcaaaggtc cggcctgcca gggtcggcga ctcgaggatt ccttgttcaa
     4801 ccaggctgga ttcgatttcg gtcataaatt gtcgacaatc tagatggaaa gtgctgtata
     4861 ttggccggaa tatgcatttt tacgatgtta ttgtcaacaa tatgggtgta agattcccgg
     4921 ctgacgacgg cgtaaatcgc cccgaagcca gtgttggcgg ggcaaaccgg gttttaattt
     4981 tcagaatgaa taaatttaat agtcaagcgg gcggttagcg gttctaacca ataatgggta
     5041 tagttaaatg tctatcccgg cacgacccgg ctttcgaccg gggatttatc cgttaacgca
     5101 aaattcgaac ctcgtttaac ggttggcacg aagaattgtt gaggacacag ctatgagcga
     5161 tgaaaccgta accctgatcg acgacacgac cggcaaggaa atcaagttac ctttgtatac
     5221 tccgaccgcg ggtcccaagg ttatcgatat cggcaagctt tacaaggagc tcggttattt
     5281 cacctacgac cccggcttca tgtcgaccgc aagctgctcg agcgcgatca cttttctcga
     5341 cggcggcaag ggcgtgctgc agtaccgcgg ctacccgatc gagcagctgg ccgagcacag
     5401 ttcctacctc gaggtctgct atctgctgct caacaaccgc ctgccgacga agagcgagct
     5461 cgaccacttc gagaacgatg tcacccggca caccatgctg cacgaggcga tgatccgctt
     5521 cttcaccggc tttcgccgcg acgcgcaccc gatggcgatc atggtcggct gcgtcggcgc
     5581 gctggccgcg ttctaccacg accatatgga tatacatgat gccgagcatc gcatgatcac
     5641 cgcctaccgg ctggtggcca agatgccgac catcgcggcg atgagttatc gctacgccaa
     5701 cggcaagccg ttcgtctacc cgcgcaacgc gctttcctat accgagaact tcctctacat
     5761 gatgttctcg atgccgtcgg aggactacac gccggacccg ctgctcgcca aggcgctcga
     5821 cctgatcatg atcctgcatg ccgaccatga gcagaacgca tcgacctcga ccgtgcgcct
     5881 cgccggctcc tccgacgcca acccgttcgc ggccgtcgcc tcggggatcg ccgcgctctg
     5941 ggggccggca cacggcggcg ccaacgaggc cgtgatccgc atgctggtcg acatcaacga
     6001 gtccggcaag ccgctgcggg atttcgtcaa ccgcgccaag gacaagaacg acccgttccg
     6061 cctgatgggc ttcggccatc gcgtgtacaa gaattacgat cctcgcgcca agctgatccg
     6121 cgagatttgt cacaagctgc tcaacgacat ggagactagc ggcgaacaga agaaattgct
     6181 ggagaccgcg atggcgctgg aaaaggttgc cctcgaggac gactatttta tcgagcgcaa
     6241 cctgtacccg aacgtcgatt tttactccgg tatcatcttc ctcgccatgg gcatcccgat
     6301 gaatatgttc acggtgatct tcgcgatggc gcgcaccatt ggctggattt cacagtggaa
     6361 tgaaatgatg gaagacgagg atgccaagat cggccgcccg cgccagctgt acaacggcgc
     6421 catcgatctc gagtacctgc ccattgacaa gcgctgagac gagacgcata tagaaataat
     6481 ttccggctaa ccgagagaag acaacatgct tgatgcgtac cgcgaacacg tcgaggcgcg
     6541 cgcggcagaa ggcgttccgc ccaagccgct cgacgcgcaa caaaccgcag aactggtcga
     6601 cctgctgaaa aatccaccgg cgggcgagga gcagttcctc gtcgagttgc tgacccggca
     6661 ggtgccggcc ggtgtcgacg aggcagccta cgtcaaggcc ggtttcctgg ccgcgatcac
     6721 ccgcggcgag gcgcagtcac cgctgatcga ccgggtgcgg gcggtcgaaa tcctcggcac
     6781 catgctcggc ggttacaaca tcgagccgtt gatcgcct
//
LOCUS       DATCZV010000135         3005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_221559, whole genome shotgun sequence.
ACCESSION   DATCZV010000135 DATCZV010000000
VERSION     DATCZV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221559"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VIW27_02975"
     CDS             <1..280
                     /locus_tag="VIW27_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738658.1"
                     /translation="SRVEKAASLNVPAGTVTGKRGGVIFLSTVNIAQTNKRKVFNKNN
                     YWKNFAPSIDTHEICTCEAGLRSARRFSIRRQRSKFDQLCKSLKLKEK"
     gene            540..2735
                     /locus_tag="VIW27_02980"
     CDS             540..2735
                     /locus_tag="VIW27_02980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5738659.1"
                     /translation="MPRPRYARFAIPLPLYRVFEYSVDDADEIVAGMRYRLPFANRTR
                     TGVLLDHGESSDLDPARVKPVLQRLDPEPVLDAHLMSLARWLADYYLQPLGEVVFTCL
                     PSRLRGAQPHVTTRVKCWRLTETNPAEIEALRRRSPRQYEICSALQTRPAGMTALELR
                     QLNPNWHDAVRALEGKGLLRWQWAEDAQPAPPSGPLPQPSAEQAAILAELEPRLDRFG
                     VHLLDGITGSGKTEIYLRLIRSRLDAGQQVIYLVPEIGLTGQLLERVRQRFGGGFEVS
                     HSGLTDLQRYRAWDRFRRGEARIMLGTRSSLFSQCQRLGLIIVDEEHDTSYRQEDGVR
                     YHARDVAIKRAQMLGIPVVLGSATPSLESLHNCARETWHRYYLDRRPTRFPPPRLKLL
                     DVRDSRLEFGLSAAALALVERHLTQGGQVLVYLNRRGYSPVVMCHDCGWQALCRHCDS
                     RLTLHRSVNCLLCHHCGYRQPVPVACPECDNRELGQHGVGTEQLEHGLASRFAGIPVL
                     RIDRDVVASGEALEKRLQALREGAPCILVGTQMIAKGHDYPAITLAVVLEADQALFSA
                     SYRASERLLQTLFQVSGRSGRGDREGEAVVQTRFTEHPLMQAVLRRSYREIAVELLSE
                     RENYGFPPCARVVMFRADAVELDEALSILGRIRTLLEQAADFGGVDCIGPMPALMTRR
                     VSRYRAQLCLISRQYRVLRSVLDEAMPAITELPGSARVNWSVDVDAYDL"
     gene            2856..>3005
                     /locus_tag="VIW27_02985"
     CDS             2856..>3005
                     /locus_tag="VIW27_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738660.1"
                     /translation="MSDLIAQALRALEQAAQIPAGLDADIQVTPSKDASQGDFASNIA
                     LMLARR"
BASE COUNT          573 a          929 c          916 g          587 t
ORIGIN      
        1 gtctcgagtg gaaaaagcgg ccagtttaaa cgtccccgcg ggaactgtca ccgggaaacg
       61 cgggggggtc atttttttat ccacagtaaa tatagctcaa accaataaaa gaaaggtctt
      121 caataaaaat aattattgga aaaacttcgc tccaagtatt gacacgcatg agatatgcac
      181 atgcgaagca ggactgcgaa gcgctcgccg attttcgatt cgccggcagc ggtcaaaatt
      241 tgaccagctt tgcaagtcat tgaaattgaa ggagaaataa aatagatgca aaaatgaaca
      301 atctaaacaa agtccagcgg aaatgcggct tgcaggactt tttttgcaat tcatacacag
      361 acttatccac agaaaatgtg gataaaatta attgtcatga tttcttaaca atttagcatt
      421 tgataactcg aaataacatt aagaaagcgc gataagtgaa ttcgacgttt tgccgctgtc
      481 gatggctgag cctgtccctg gattcgagta tgattgccgt ttcgattcga gttacgcgcg
      541 tgccccgacc ccgttacgcc cgcttcgcca ttccgctgcc gctgtaccgg gtattcgaat
      601 acagtgtcga cgacgccgac gagattgtcg ccggcatgcg ttaccgcctg cctttcgcca
      661 atcgcacccg taccggtgtg ctgctcgatc acggcgaatc cagcgacctc gatccggcgc
      721 gcgtcaaacc ggttttacag cggctcgatc ccgaaccggt gctcgatgcg cacctgatgt
      781 cgctggcacg ctggctcgcg gactattatc tgcagccgct cggcgaagtc gtatttacct
      841 gtctgccctc ccgtctgcgc ggcgcccagc cgcacgtcac cacgcgggtc aaatgctggc
      901 ggctcaccga gactaacccc gcggagatcg aggcgctgcg tcgccgttcg ccacgccagt
      961 acgaaatctg tagcgcattg caaacgcgac cggcggggat gaccgcgctc gaactcaggc
     1021 agctcaaccc caactggcac gatgcagtca gggcgctcga gggtaagggt ctgctgcgct
     1081 ggcagtgggc cgaggacgcg cagccagcgc cgccgtccgg gccgctaccg caaccgagcg
     1141 ccgagcaggc cgcaatcctc gccgagctcg aacccaggct cgaccgcttc ggcgtgcacc
     1201 tgctcgatgg cataaccggc agcggcaaga ccgaaatcta cctgcgcctg atacggtcgc
     1261 ggctcgatgc cggccagcag gtgatctacc tggtacccga gatcggtctc accggccagt
     1321 tgctggaacg cgtgcggcaa cgcttcggcg gcggcttcga agtctcccac tcgggcctca
     1381 ctgacttgca gcgttaccgt gcctgggatc gttttcggcg gggcgaagcg cgcatcatgc
     1441 tcggtacccg ctcgagcctg ttttcgcaat gccagcggct cggcctgatt atcgtcgacg
     1501 aagagcacga cacttcctac cgccaggagg acggggttcg ttaccacgcg cgcgacgtgg
     1561 ccatcaagcg cgcccagatg ctcggtatac cggtggtgct cggttccgcc acgccgtcgc
     1621 tggagagcct gcacaattgc gcgcgcgaga cctggcatcg ttactacctg gatcggcggc
     1681 cgacgcgttt tccgccgccg cggctgaaat tgctcgacgt gcgcgacagc cggctcgaat
     1741 tcgggcttag cgcagcggcg ctggcactgg tcgagcgcca cctgacgcag ggtggccagg
     1801 tgctggtcta tctcaatcgc cgcggctatt cgccggtggt gatgtgccac gattgtggct
     1861 ggcaggccct gtgccggcat tgcgattcgc ggctaacgct tcaccgctcg gtgaattgcc
     1921 tgctttgtca ccattgcggc tatcgccagc cggtccccgt tgcctgtccc gaatgcgaca
     1981 accgcgaact cgggcagcac ggcgttggta ccgagcagct cgagcatggg ctcgccagcc
     2041 gcttcgcggg aatcccggta ctgcgcatcg atcgcgacgt ggttgcttcg ggcgaggcgc
     2101 tcgaaaaacg cctgcaggcg ttgcgcgaag gtgcgccctg catcctggtg ggtacgcaaa
     2161 tgatcgccaa gggacacgac tatccggcga ttacgctggc cgtggtgctg gaggcggacc
     2221 aggcgctgtt cagcgcgtcc taccgcgcgt cggagcgcct gctgcaaacc ctcttccagg
     2281 tatcggggcg ttccggccgc ggcgaccgcg agggcgaggc ggtggtgcag acgcgtttca
     2341 ccgagcatcc gctgatgcag gcggtattgc gccgcagtta ccgagagatc gccgtcgagc
     2401 tgctgagcga gcgcgaaaat tacggctttc cgccctgcgc ccgcgtagtc atgtttcgcg
     2461 ccgacgcggt ggaactcgat gaggcgctgt cgatcctcgg gcggatcagg acgctgctcg
     2521 aacaggccgc cgacttcggc ggtgtcgatt gcatcggccc gatgccggcg ctgatgacgc
     2581 gccgcgtcag ccgctatcgc gcgcagctgt gcctgatctc gcggcaatac cgcgtgctgc
     2641 gctcggtgct cgacgaagcc atgccggcga tcaccgaatt gccgggctcg gcgcgcgtca
     2701 actggagtgt cgatgtcgat gcctacgatc tgtgagccgc cggccgggca aatccacgat
     2761 ccggcgaaat cacaggtggt agggggtcgc cgcctcgggt aaaatcgcgc ttcatttctc
     2821 ccgtccccaa cgagtcccgg ttcttgaagc aagtcatctc agatctgatc gcgcaggcat
     2881 tgcgagcgct cgagcaggct gcgcaaattc ccgccggtct cgatgccgat atccaggtga
     2941 cgccgtcgaa ggatgccagc cagggtgatt tcgcgtcgaa tatcgcgctg atgctggccc
     3001 ggcgc
//
LOCUS       DATCZV010000136         4802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_327326, whole genome shotgun sequence.
ACCESSION   DATCZV010000136 DATCZV010000000
VERSION     DATCZV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327326"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..659)
                     /locus_tag="VIW27_02990"
     CDS             complement(51..659)
                     /locus_tag="VIW27_02990"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY5738661.1"
                     /translation="MPDLDRLTIDDIRRRLHAQRGTTYDVPPELLGAEAREAAVLVPF
                     VRIDDAWHILYIRRAQIDGDRHSGQVAFAGGKREAGDTSLLDTALREADEEVGIEPGD
                     VEVLGYINHHHTISEFQVRPYVGIVPWPYDLRLDPVEVAHAFTMPLAWLADESNYRTE
                     ARRHPDSQRSWPVVYFERYDGELLWGATARMTLSLIDTLRGG"
     gene            complement(652..840)
                     /locus_tag="VIW27_02995"
     CDS             complement(652..840)
                     /locus_tag="VIW27_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY5738662.1"
                     /translation="MDTEAAPIASPCIGVCTLDDDDVCVGCYRSIDEISGWRTAGDDE
                     KRAIVDRAAARGRILRRA"
     gene            complement(830..1729)
                     /locus_tag="VIW27_03000"
     CDS             complement(830..1729)
                     /locus_tag="VIW27_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5738663.1"
                     /translation="MLPIYPVTHVEPVFRPPSEAASLILQVTNGCSWNRCTYCEMYTD
                     PQKRFRVRPETEVLTEIRACAERGLAPRRVFLADGDALVLSMRRLRAILRAIRRELPS
                     VTRVGAYCLPANLQRKHDDELAELRELGLGIVYVGAESGDDDVLRQVAKGETRDTAVA
                     ALLRARSAGIRTSVMILNGLGGRRLSRRHALASAQLVNQAQPDYLATLVLTFYRGRER
                     FVAGFDDGFDELDTVGLCEEMRIFIDATELDSTIFRSDHVSNHLVLKGVLGRDKQRLL
                     RHIDESIEFFRQHPEFEHGDYGY"
     gene            1904..3016
                     /locus_tag="VIW27_03005"
     CDS             1904..3016
                     /locus_tag="VIW27_03005"
                     /inference="COORDINATES: protein motif:HMM:NF009133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoniopropionate demethylase"
                     /protein_id="HEY5738664.1"
                     /translation="MADKVLSISRRTRATPFSRRVEAAGVRGYTVYNHMLLPTFFESN
                     EFDYWHLCTDVQVWDVSCERQVEVCGPDSRRLVQLMTPRDISRCAIGQGLYAPLCDRD
                     GGIVNDPVLIKLADDHWWISIADSDVVLWADGLAQGYGLDVRVREADIWPLAVQGPKA
                     EQLVARVFGDQVPDIRFFRSRMLPFRGREMMVMRSGYSKQGGFEIYLNDADLAEPLWD
                     ELFDRGQDLNVRAGGPNLMERIESGLLSYGNDMTRDNTPFECGLEAFIDLDANIDAIS
                     LPALRRIHGKHQKQLMGLVFDQMVSIDDFEIEADGSLVGDIRSHSWSPRHSCYLAMAM
                     MNREFLGKNSKIDLNGKIAHIVDLPFDFATVREVHK"
     gene            3013..4320
                     /locus_tag="VIW27_03010"
     CDS             3013..4320
                     /locus_tag="VIW27_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY5738665.1"
                     /translation="MSRSTYLKPDTVALHTGYVPESEYGARAVPVYQTTSYVFESVSE
                     GSSLFNVEQGGHIYSRISNPTVAVLEQRIAALEGGSGAICTASGMSALFMTFISLCSA
                     GDHLISATQIYGSTATLLKHTIKRLGIDCTFVPINDEQALRDAIQPNTKLVFCESIGN
                     PGLEIANIPGIAAIAHESGIPLVVDATFATPSLCRPIDHGADVVVHSVTKWMGGHGIA
                     IGGVVVDGGQFDWSASGRFPELTEPYAPFHGIRFWEEFGPSALVMKMRAESMRDIGAT
                     MSPHNAFLILQGLETLNLRMKQHVGNAVELLAWLQQQEAVTWTNYPSMASNPSHVFAD
                     ALFPGGAGSMLCFGVTGGRAGGAAFIDALQLASNLANVGDARTLVLHPATTTHSRLTA
                     EDMKAAGISEDLVRVSVGIEDITDIEADFARGLKAAQRVARSA"
     gene            4320..>4802
                     /locus_tag="VIW27_03015"
     CDS             4320..>4802
                     /locus_tag="VIW27_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5738666.1"
                     /translation="MYIEVRGQQTYAATGGKDFDPALPTVMFVHGSGLDHRSWALQTR
                     WFAYNGYAVLAPDLPGHSLSAGEPLEAIEDAGSWLADFIRAAGADGVHAVGHSQGFLA
                     VLELAHQAPQLLKSITGIGTAGAIPVNPALIDAASKSAATAAEMLLMWGFGTDAQYGI
                     S"
BASE COUNT          871 a         1536 c         1534 g          861 t
ORIGIN      
        1 gcagtgatgg cttcgagatt gcggtattcg ctcgccggca tcttccctcc tcagccgccg
       61 cgcagggtgt cgatcagcga cagggtcatg cgcgcggtcg caccccacag cagctcaccg
      121 tcgtagcgct cgaagtagac caccggccag gagcgctgcg aatcgggatg gcggcgcgcc
      181 tcggtacggt aattggactc gtcggcaagc caggcaagcg gcatcgtaaa ggcatgcgcg
      241 acctcgaccg gatcgaggcg caggtcatag ggccagggaa cgatgccgac ataaggtcgc
      301 acctggaatt cactgatggt gtgatgatga ttgatatagc cgagcacctc gacatcgcca
      361 ggttcgatcc cgacttcctc gtcggcctcc cgcagcgcgg tgtcgagcag actggtatcg
      421 ccggcttcac gcttgccgcc ggcgaaagcg acctggccgc tgtgacggtc gccgtcgatc
      481 tgcgcacggc gaatgtagag gatatgccag gcgtcgtcaa tgcgcacgaa aggtaccagc
      541 acggccgctt cacgggcttc ggcgccgagc agttccggcg gcacatcgta agtggtgccg
      601 cgctgcgcgt gcaggcgccg gcgaatatcg tcgatcgtca ggcgatcgag atcaggcacg
      661 ccgcagaatc cggccgcgcg ccgcggccct gtcgacgatt gcgcgcttct cgtcgtcgcc
      721 ggcggtacgc cagccactga tttcgtcgat gctgcggtaa cagccgacac agacatcgtc
      781 gtcgtcgagc gtgcaaaccc cgatgcacgg ggacgcgatc ggagccgctt cagtatccat
      841 agtcgccatg ttcaaattcc gggtgttgtc tgaaaaattc gatcgattcg tcgatgtgcc
      901 gcagcagccg ctgcttgtcc ctgccgagca cgcctttcag caccaggtga ttagacacgt
      961 gatcgctgcg aaagatcgtc gagtcgagct cggtcgcatc gataaatatt cgcatttcct
     1021 cgcacagccc gacggtatcg agttcgtcga agccatcgtc gaagccagcc acgaagcgct
     1081 cgcgcccacg gtagaaagtc aataccaggg ttgccaggta atccggctgt gcctggttga
     1141 ccagttgcgc cgaagccagt gcgtgccgac ggctcaggcg gcggccgccg agaccgttaa
     1201 ggatcatcac tgaggtcctg ataccggcgc tgcgggcacg cagcagcgct gccacggcag
     1261 tgtcgcgggt ttcacccttg gcgacttgcc tgagcacgtc gtcgtcgccg ctttccgcgc
     1321 caacgtagac tatccccagt cccagctcgc gcagctcggc cagttcgtca tcgtgcttgc
     1381 gctgcaggtt cgccggcaga cagtaggccc cgacccgcgt caccgaaggc agctcacggc
     1441 gaatcgcgcg cagaatggcg cgcaagcgcc gcatcgacag gaccagcgcg tcgccgtcgg
     1501 cgaggaatac gcggcgcggc gcgagtcccc gctcggcgca ggcgcggatt tcggtcagca
     1561 cctcggtctc gggccgcacg cggaaacgct tttgcggatc ggtgtacatc tcgcagtagg
     1621 tgcagcggtt ccagctgcag ccgttggtga cctgcaggat caacgaagcg gcttcgctcg
     1681 gcgggcgaaa caccggctcc acatgggtaa cgggataaat cggcaacatt tcgcgtgtag
     1741 gctattgaat cgcgccgctc gagacaagcc gagcgcaccc cgactgtggg tcgtggacgg
     1801 cgcgtagggt attattttag ccgctatttg tcagcgaaat aaaaaaagtc tagcattgcg
     1861 ccctcgacgc ccgctgccca accgatgacc gaggtacctt gctgtggccg acaaagtatt
     1921 gagtatctcc cggcgcaccc gtgcgacccc gttcagccgc cgtgtcgagg ccgccggtgt
     1981 gcgcggttac actgtttaca accacatgct gctgccgacg ttcttcgaaa gcaacgaatt
     2041 cgattactgg cacctgtgca ccgacgtcca ggtctgggac gtgtcctgcg agcgccaggt
     2101 ggaggtctgc ggcccggaca gccggcgcct ggtgcaactg atgacgccgc gggacatctc
     2161 gcgctgcgcg atcggccagg gcctgtatgc gccgctgtgc gaccgcgacg gcggtatcgt
     2221 caacgacccg gtgctgatca agctcgccga cgaccactgg tggatttcga ttgccgacag
     2281 cgacgtcgtg ctgtgggcgg acgggctcgc gcagggctac ggtctcgacg tgcgggtacg
     2341 cgaggccgat atctggccac tcgcggtgca gggtccgaaa gccgagcagc tggttgcccg
     2401 cgtgttcggc gaccaggtgc ccgacattcg ctttttccgc tctcgaatgc tgccctttcg
     2461 tggccgcgaa atgatggtca tgcgctccgg ttacagcaag cagggcggct ttgaaattta
     2521 tctcaacgat gccgacctgg ccgagccgct gtgggacgaa ctgttcgacc gcggccagga
     2581 cctgaacgtg cgcgccggag ggcccaacct gatggaacgc atcgagagcg gcctgctgtc
     2641 ctacggcaac gacatgaccc gcgacaatac cccgttcgag tgcgggctgg aggccttcat
     2701 cgatctcgac gccaacatcg acgcgatcag cctgccggcg ctgcgccgca tccacggcaa
     2761 acaccaaaag cagctcatgg ggctggtctt cgaccagatg gtgtcgattg acgatttcga
     2821 gatcgaggcc gacggcagcc tggtcggcga cattcgttcg cactcctggt cgccgcgcca
     2881 ctcctgctac ctggcgatgg cgatgatgaa ccgggaattc ctcggcaaaa attcgaaaat
     2941 tgatttaaac ggtaaaatag cgcatatcgt cgacctgccg ttcgactttg ctacggtccg
     3001 cgaggtgcat aaatgagtcg ttccacctac ctgaaacccg atacggtcgc gctgcatacg
     3061 ggctatgtcc cggaaagcga gtacggcgcg cgcgcggtgc cggtttacca gaccacctcc
     3121 tatgttttcg agagcgtcag cgagggttcg tcgctgttca acgtcgaaca gggcgggcat
     3181 atctacagcc gcatcagcaa cccgaccgtg gcggtgctcg agcagcgcat tgccgcactc
     3241 gagggcggca gcggcgcgat ctgcacggcg tcgggcatga gcgcgttgtt catgaccttt
     3301 atctcgctgt gctccgccgg cgatcacctg atctcggcga cgcagatcta cggctccacc
     3361 gcgaccctgc tgaagcacac catcaagcgt ctgggcatcg attgcacctt cgtgccgatc
     3421 aacgacgagc aggcgctgcg cgacgcgatc cagccgaaca ccaagctggt gttctgcgaa
     3481 tcgatcggca acccgggtct cgaaatcgcc aatatccccg ggatcgccgc gattgcacac
     3541 gagtccggta ttccgctggt cgtcgacgca accttcgcca cgccgagcct gtgccggccg
     3601 atcgaccacg gcgccgacgt cgtcgtgcac tcggtgacca agtggatggg cggccatggg
     3661 atcgcgatcg gcggcgtggt cgtcgacggc ggccagttcg actggtcggc gagcggccgc
     3721 tttcccgagc tgaccgaacc ctacgcgccg ttccacggca ttcgcttctg ggaagagttc
     3781 ggaccgtcgg cgctggtaat gaagatgcgc gccgaatcga tgcgcgatat cggcgcgacg
     3841 atgagcccgc acaacgcgtt cctgatcctg cagggtctcg aaaccctgaa cctgcgcatg
     3901 aagcagcacg tcggaaatgc cgtcgagctg ctcgcctggc tgcagcagca ggaagccgtc
     3961 acctggacca attacccgtc gatggcatcg aatccgagcc acgtattcgc cgacgcgctg
     4021 tttcctggcg gcgccggctc gatgctgtgc tttggtgtca ccggcgggcg cgccggcggt
     4081 gccgcgttca tcgatgcgct gcagctggcg tcgaacctgg ccaacgtcgg cgacgcgcgt
     4141 accctggtac tgcacccggc gaccaccacg cactcgcggc tgaccgccga ggacatgaag
     4201 gccgccggca tcagcgagga cctggtgcgg gtttcggtcg gcatcgagga catcaccgat
     4261 atcgaagccg acttcgcacg cggcctgaag gccgcgcaac gcgtcgcgag gtcagcctga
     4321 tgtatatcga ggtacgcggt caacagactt acgcggccac cggcggcaag gatttcgacc
     4381 ctgccctgcc aacggtgatg ttcgtgcatg gcagcggcct cgaccaccgc agctgggcgc
     4441 tgcagacacg ctggttcgcc tacaacggtt acgcggtgct ggcaccggat ctgcccggtc
     4501 acagcctgtc ggccggcgag ccgctggagg ctatcgagga tgcgggctcc tggctggcgg
     4561 atttcattcg cgccgcggga gccgacggcg tgcacgcggt cggccactcg cagggatttc
     4621 tggcggtgct ggaactcgcg caccaggcgc cgcaattgct gaaatcgatc actggtatcg
     4681 gtaccgccgg agcgataccg gtcaacccgg cactgatcga cgcggccagc aagagcgccg
     4741 ccacggctgc cgagatgctg ttgatgtggg gtttcgggac cgatgcgcag tacggcatca
     4801 gt
//
LOCUS       DATCZV010000137         4918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_327334, whole genome shotgun sequence.
ACCESSION   DATCZV010000137 DATCZV010000000
VERSION     DATCZV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327334"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VIW27_03020"
     CDS             complement(<1..461)
                     /locus_tag="VIW27_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY5738667.1"
                     /translation="MTTSAELREKFLSYFESHGHTIVPSSPLVPGNDPTLLFTNSGMV
                     QFKDVFLGTDKRSYSRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGEYF
                     KKEAIHYAWDFLTNVIGLPAEKLWVTVYAEDDEAAEIWLHDIKVDPRRFTR"
     gene            complement(805..1209)
                     /locus_tag="VIW27_03025"
     CDS             complement(805..1209)
                     /locus_tag="VIW27_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY5738668.1"
                     /translation="MDLLARREHSTEELRRKLKAREFDQHAVELALEALQHDGLLSDA
                     RFAESFVHRRFNAGMGPMKIAYELRQKGIDAALADECLAVYADRWQESMLRERARRFG
                     DAIPGDYGARMKQARFLQNRGFSPESVMRLFR"
     gene            complement(1382..2452)
                     /gene="recA"
                     /locus_tag="VIW27_03030"
     CDS             complement(1382..2452)
                     /gene="recA"
                     /locus_tag="VIW27_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY5738669.1"
                     /translation="MDDNKQKALAAALGQIEKQFGKGSVMRLGDSGVDMAMGVISTGS
                     LSLDVALGVGGLTRGRVVEVYGPESSGKTTLALQVVAECQKLGGAAAFVDAEHALDPS
                     YADKLGVNIDDLLVSQPDTGEQALEITDMLVRSGAVDVVVVDSVAALTPKAEIEGDMG
                     DSHMGLQARLMSQALRKLTGNIKRSNTMVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRIGAIKRGDEVIGNETRVKVVKNKVAPPFRQCEFEILYGEGSSREGELIN
                     LGVKQGLIEKSGAWYSYNDERIGQGKDNVRAFLRENPEIAEDIDQRLRAELLPKPRSA
                     ERIDSDATEPDPRVEPEMVEVP"
     gene            2693..3472
                     /locus_tag="VIW27_03035"
     CDS             2693..3472
                     /locus_tag="VIW27_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738670.1"
                     /translation="MSNNRPDLSTTQILLLDASIAAAAAVILLAPGSTAPGSPAGQLA
                     AAGGALLLLAPLLFVVMKRSASSASPPAWFIVHVLASLAGSCLILVHAKAGDWLTPPG
                     LLLLLLVLLVMQGSLLRAVLSRGFSLLFARSSQANGFAAPAALDKAALQRLIERKTAL
                     LQDLDPAADEALFSPALEHWLRSPWQSLRYQWLAEREARMVGARAGAGPALAWSRRLH
                     LLLALGFYLGLLAHVVVVLFFAGYAADGGAVDWWHVTAWGD"
     gene            3476..>4918
                     /locus_tag="VIW27_03040"
     CDS             3476..>4918
                     /locus_tag="VIW27_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5738671.1"
                     /translation="MTDPHTQLALMIDLERCTGCKSCEAACKQTNALGPNSYRNRVIW
                     FDEQQPDNGENAPARLDFLTVTCQQCERPACLRACPVYPKAISKDALTGVVSIDEQRC
                     TGCGECALSCPYGAIGYNAEQHHAVKCDLCAERRADGAGPACAFVCPTRAISFGTRAA
                     LLETARRDGRELLDTDHFLQQPATIYLRRPRDAGHESSTHSTGTTPALMRDHSSRVSI
                     EPGNARGSYRRAEKSVLLEQAERIVPGGCNICFNGCPVKFHLRGNRVVNVYGNDEDPQ
                     FQGRICPKSQMTLQLYDNPHRLTTPLKRVGPRGSDSFIAISWEQALSEIAARLAAVRT
                     RHGSEALAIQSGSRTGVLTIIGAIPLFAGLWGTRNVLTTEPYCDLGKGVALKLTQGAG
                     FMCSVFTEDDIGRAGAYVYIGDNQAETRPVNFGLLNDWRLRRGAKMIVVDPRLTATAS
                     KADAWLAIRSGTDMALGLGIIHYLFAHDLHD"
BASE COUNT          896 a         1643 c         1461 g          918 t
ORIGIN      
        1 atgcgggtaa atctccgcgg gtccaccttg atgtcgtgca gccagatctc cgcggcttcg
       61 tcatcttctg cgtagaccgt cacccagagt ttttccgcgg gcaggccgat gacattggtc
      121 aggaaatccc aggcatagtg aatcgcttcc ttcttgaaat attcgccaaa gctgaaattg
      181 ccgagcattt cgaaaaacgt gtggtgacga gccgtgtagc cgacgttctc gaggtcatta
      241 tgcttgccgc cggcgcgcac acagcgctgc gcggtcgcgg cgcggctata gctgcgcttg
      301 tcggtgccga gaaacacgtc cttgaactgc accatgccgg aattggtgaa caacagggtg
      361 ggatcgttgc cgggcaccag cgggctcgac ggcacgatgg tatgcccatg tgactcgaaa
      421 tagctgagaa acttttcccg aagctcggcg ctggtagtca ttgactcgcg atcactaatt
      481 tacaaaaccg atgacgcccg gaaaatcccg ggcgttcacg acccgcgcat cccgacgcac
      541 gcgctacagc ggcggtgaaa tgggcggact cgcctgaaaa tagcacagtc ggatcagccc
      601 cccggtggca ggcaatcgaa accacaggaa aacgctctgc cgggtaaccg aatccagcta
      661 tagcagcgct tgtggttcga atcccgtccc gcgtctactc ggccggacgc gcctcgacca
      721 ggaaccacta cccctgtcgg tcaatgccgg cgtcgcggcc ctgttcccct gcgttactgc
      781 cagggcactg gtgcaaatat caggttatcg aaataatcgc atgactgact cgggagaaaa
      841 acccctattt tgtaaaaaac gcgcctgctt catacgcgcg ccgtagtccc ccggaatcgc
      901 atcgccaaaa cggcgcgctc gctcgcggag catcgattcc tgccagcgat ccgcgtaaac
      961 cgcgagacat tcatcggcaa gcgcagcgtc gatgcccttt tgccgcagtt cataggcgat
     1021 cttcatgggt cccatcccgg cattaaaacg ccggtgcacg aaagattcgg cgaagcgcgc
     1081 gtccgacagc aacccgtcgt gctgcaacgc ctcgagagcc agctcgaccg cgtgttggtc
     1141 gaattcccgt gccttgagct tacggcgcag ttcctcggta gaatgctcgc gccgtgccag
     1201 taaatccata gcccggctac gaatcgctac actcgaccct tccgtcatca cggccctccc
     1261 ggatcagcga cgccgcgcac cgcccgaacg cgcatagccg cgtaaaaaaa aagccttcgg
     1321 agcatgatgt ctccgaaggc attgccagtt tcgcaacatc tggtggatag ggataggtag
     1381 gctagggaac ttccaccatt tcgggttcga cgcgagggtc gggttctgtt gcatcactgt
     1441 caatccgctc cgccgacctg ggcttcggca gcagttcggc gcgcaggcgc tgatcgatat
     1501 cttcggcgat ctcggggttt tccctgagga aggcgcgcac attgtccttg ccctggccga
     1561 tccgctcgtc gttatagctg taccaggctc ccgacttctc gatcagcccc tgcttgacgc
     1621 cgagattgat gagttcgccc tcgcgcgacg aaccttcgcc gtagagaatt tcgaactcgc
     1681 actgcctgaa gggcggggcc accttgttct tgaccacctt gacgcgggtt tcgttaccga
     1741 tcacctcgtc accccgcttg atcgccccta tgcgccgaat gtcgaggcgc acggaggcat
     1801 agaactttag cgcgttgccg ccggtcgtgg tttccgggtt gccgaacatg acaccgatct
     1861 tcatacgaat ctgattgatg aatatcacca tcgtattcga gcgcttgata ttgccggtca
     1921 gcttgcgcag cgcctgcgac atcaggcgag cctgcagacc catgtgcgaa tcgcccatgt
     1981 cgccctcgat ttccgccttc ggcgtcagcg ccgcgaccga gtcgacgacc accacgtcga
     2041 ccgcgccgga gcgcaccagc atgtcggtga tctccagtgc ctgctcgccg gtatcgggct
     2101 gcgataccag caggtcgtcg atattgacgc cgagcttgtc ggcatagctc ggatcgaggg
     2161 catgctcggc atcgacgaag gccgccgcac cgcccagttt ctggcactcg gcgaccacct
     2221 gcagcgcgag ggtcgtcttg cccgaggatt ccgggccata gacctcgacc acgcgaccgc
     2281 gcgtcaaacc accgacgccc agcgccacgt cgagcgacag cgaaccggtt gaaattacgc
     2341 ccatggccat atcgactccc gaatctccaa gccgcatgac cgatcccttg ccgaattgct
     2401 tttcgatttg ccccagcgcc gcggcgagcg ccttttgctt gttatcgtcc attgtttacc
     2461 taccctatgt tgtggtttcg cggattatca cacaccaggg cgagaacata aagagaactt
     2521 cgatattttc gcgggttacc ctaccaaccc gtatcccggt gactgccgcc cattatcgcg
     2581 ggttactggc tcatatcgtg agccagtaaa aaaataatat cgctcgtgtg ccggcgcggt
     2641 tgaaccgcgc gcgccgcaag gctagtcttg acgcaaaata accggcggcg ccgtgagcaa
     2701 taatcgaccc gatctctcga caacacagat tctgctgctg gacgcatcga tcgccgccgc
     2761 cgcggccgtc attctgctgg cgccaggctc aacggcgccg ggctcgccgg ccggccagct
     2821 ggccgccgcg ggcggcgcac tgctgctgct cgcaccgctg ctgttcgtag tcatgaaacg
     2881 ctcggccagc agcgccagcc cgccggcctg gttcatcgtc catgtgcttg cctcgctggc
     2941 cggtagctgc ctgatcctgg tgcatgccaa ggccggcgac tggctgacgc caccgggcct
     3001 gctgctgttg ctgctggtat tgctggtaat gcagggcagc ctgctgcgcg cggtactgtc
     3061 gcgcggtttc agcctgctgt tcgcgcgcag cagccaggcg aacggtttcg ctgcgcccgc
     3121 cgccctcgac aaggccgcgc tgcagcgcct gatcgagcgc aagaccgcgc tgctgcaaga
     3181 cctcgatccc gcagccgacg aggccctgtt ctcgccggcc ctcgaacact ggctgcgcag
     3241 tccctggcag tcgctgcgct accagtggct ggcagaacgc gaggcgcgca tggtcggcgc
     3301 gcgcgctggc gccggtccgg cgctggcctg gtcgcggcgg ctgcacctgc tgctcgcact
     3361 cggcttctat ctgggcctgt tggcgcatgt cgtcgtggtg ctgtttttcg ccggctacgc
     3421 ggccgacggc ggcgccgtcg actggtggca tgtcaccgcc tggggagact gacgcatgac
     3481 cgatccgcac acccagctcg cgctgatgat cgatctcgag cgctgcaccg gctgcaagag
     3541 ctgcgaggcc gcctgcaaac agaccaacgc gctcggcccc aacagttacc gcaaccgcgt
     3601 aatctggttc gacgagcaac aaccggacaa cggtgagaac gcgcctgcaa ggctcgattt
     3661 tctgaccgtg acctgccagc agtgcgaacg ccctgcctgt ctgcgcgcgt gcccggtcta
     3721 tccgaaggcg atctccaagg atgcgcttac cggcgtggtg tcgatcgacg agcagcgctg
     3781 caccggctgc ggcgaatgcg cgctatcgtg tccctacggc gcgatcggct acaacgccga
     3841 acagcaccac gcggtcaagt gcgacctgtg cgccgagcgc cgtgccgatg gcgccgggcc
     3901 ggcctgcgct ttcgtctgtc cgacacgggc gatcagtttc ggcacgcgcg cggcgctgct
     3961 ggagaccgcg cggcgcgacg gacgcgaatt gctcgatacc gaccacttcc tgcagcaacc
     4021 ggcgaccatc tacctgcgcc ggccgcgcga cgccggccac gaatcgtcaa cgcattcgac
     4081 aggcaccacg ccggcactga tgcgcgacca ttccagccgc gtctcgatcg agcccggcaa
     4141 cgcgcgcggc agctaccgcc gggcggagaa aagcgtgctg ctcgaacagg ccgagcgcat
     4201 cgtgccgggc ggctgcaaca tctgtttcaa cggctgcccg gtcaagtttc acctgcgcgg
     4261 caaccgggta gtcaatgtct acggcaacga cgaggatcca cagttccagg ggcgcatctg
     4321 ccccaagtcg cagatgactt tgcagctcta cgacaacccc caccgcctga cgacaccgct
     4381 gaagcgagtc gggccgcgcg gcagcgattc gttcatagcg atctcctggg aacaggcgtt
     4441 gtcggaaatc gccgccaggc tggccgcggt gcgcacgcgc cacggcagcg aggcactcgc
     4501 catccaatcc ggatcgcgta ccggcgtgct gaccatcatc ggcgcgatac ccttgttcgc
     4561 cggcctgtgg ggcacgcgca acgtgctgac taccgagcct tactgcgatc tcggcaaggg
     4621 cgtggcgctg aagctgaccc agggtgccgg cttcatgtgc agcgtgttta ccgaggacga
     4681 tatcggccgc gcgggcgcct acgtctacat cggcgacaat caggccgaaa cgcggccggt
     4741 aaacttcggc ctgctgaacg actggcgcct ccgccgcggc gcgaaaatga tcgtcgtcga
     4801 cccgcggttg acggccaccg cgagcaaggc cgacgcctgg ctggcgattc gctccggtac
     4861 cgacatggcg ctcggtctcg gcatcattca ttacctgttc gcgcacgacc tgcacgat
//
LOCUS       DATCZV010000138         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_539503, whole genome shotgun sequence.
ACCESSION   DATCZV010000138 DATCZV010000000
VERSION     DATCZV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539503"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VIW27_03045"
     CDS             <1..785
                     /locus_tag="VIW27_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738672.1"
                     /translation="FTWMGSTRMALATVIMSWSAIICLTGIAAWLVLEDDTPARTVNG
                     DGKAHGVGWREVMAVLRMPAVWLISMVLLATNTGFWTTYYFTPYASEVFLLSVAVAGM
                     IALGRNWMNPIAPFIAGFIADRFGISRTSTALLLIMTISFALFAITPPVASLLPLVLL
                     NIVIVALSIYALRGIYFALLDEQAIPIAVTGTAAGVASVIGFTCDIYMPIVGGAILDA
                     LPGLPGYRVLFAGASVVSALGTLAAWRLMKNRRQLLTVAAAQ"
     gene            915..2459
                     /locus_tag="VIW27_03050"
     CDS             915..2459
                     /locus_tag="VIW27_03050"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5738673.1"
                     /translation="MSDRAVRRGRKARHESPPVASAIKPGMTGGAYRPLSEHDIEQIH
                     QAVLEVLETIGIADAIPIVEEHALKQGCWMNDKGRLCFPRALVEDIIASTAKELTFMG
                     QDSRHDLHLGDQRVHTYGGGEAVTMLDVGAKKFRPSTLPDVYDAARLVDKLDNIHAFS
                     RLIVATEMTGRFACDLNTAYVSAAGTAKHTALTFGDARNVEPTLEMLYMIAGGKDKFL
                     ERPFAHGGGCAIVSPLRYSQENCEVCIESTKFGAPVWIVTAPQAGATAPAALAGTLVQ
                     VTAEALACLLMINLVVPKYPVIMGPWPFVSDLRTGAFTGGGGEEAIVSAAAGQIMNWY
                     GLVSSVGAGMTDAKSPDNQAGYEKAIAVLLAAQAGTNNVSESAGMMGSLMGLSFESLV
                     IDNDMLGAVMRTVRGIEVNEETLSVDIIRSAIAGEGHYLRQDQTLSLMRTEYEYPKLA
                     DRRTPNDWVDAGSPDIREQAAERVKEILSTHYPEYIHPDVDKKIREKFKIELPREYMF
                     ASSGRW"
     gene            complement(2475..>2985)
                     /locus_tag="VIW27_03055"
     CDS             complement(2475..>2985)
                     /locus_tag="VIW27_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEY5738674.1"
                     /translation="QKHLPLHACSISAWCSILVLITRSYRIYEIIFFIGLAGATQTVL
                     TPDAGEYGLPHFRAIQTIAAHGMIIIVMTFMTVIEGYRPTWRSIWKALFAANIYMVLV
                     TGVNLLIGSNYMYTLGKPASASILDMMGPWPWYLFWAEFLALGLFTIFYLPFAIADWR
                     AKRQVAVTT"
BASE COUNT          584 a          853 c          967 g          581 t
ORIGIN      
        1 tattcacgtg gatgggcagt acgcgaatgg cgctggcgac ggtgatcatg agctggtctg
       61 cgatcatctg cctgaccggc attgccgcat ggctggtgct cgaggacgat acgccagcca
      121 ggacggtcaa cggtgacggc aaggcccatg gggttggctg gcgcgaagtc atggcggtgc
      181 tgcgcatgcc ggcggtatgg ctgatctcga tggtgctgct ggcgaccaat accggcttct
      241 ggaccaccta ctatttcacg ccctatgctt ctgaagtgtt cctgctgagc gtggcggtcg
      301 ccggcatgat cgccctcggg cgtaactgga tgaacccgat cgcgccgttt atcgcgggtt
      361 ttattgcgga ccggtttggc atttcgcgga cttcgacagc gctgctgctg atcatgacga
      421 tcagttttgc cctgtttgcg attactccgc cggtcgcctc cctgttgccg ctggtattgc
      481 tgaacatcgt catcgtcgct ctcagcattt acgcgttacg cggtatctac tttgccctgc
      541 tggacgaaca ggccatcccg attgcggtga ccggcaccgc ggcaggcgtc gcttcggtca
      601 tcggctttac ctgcgatatc tatatgccga tcgtcggcgg tgccatactc gatgcgctgc
      661 ctgggcttcc cggctaccgt gtcctgtttg ccggcgccag cgtggtttcg gcactgggca
      721 ccctggcggc atggcgcctg atgaaaaatc gcaggcaact gctgaccgtt gccgcggcac
      781 aataataata agcaggtcat cgcctcattg gcggggggag ttgaactcta atttcaaagc
      841 ggtgagactt ggacgctatg gcccgcccaa aaaaagcggg gcgccgaagc aggtgtaaac
      901 gtggggagta attgatgagt gacagagcgg tccgcagggg acgaaaggcg aggcacgaga
      961 gcccgccggt ggcatccgcg atcaagcccg gaatgacagg tggggcctac cgccccctca
     1021 gcgagcacga catcgagcaa attcaccagg cggtgctcga ggtactcgaa accatcggta
     1081 tcgcggacgc gattccgatc gtcgaggagc atgccctgaa acagggctgc tggatgaatg
     1141 acaaaggccg gctgtgtttt ccgcgggcac tggtcgaaga catcattgcg tcgaccgcaa
     1201 aagaactgac cttcatggga caggattcgc ggcatgatct gcacctcggc gatcagcgtg
     1261 tgcacaccta tggtggtggc gaggccgtca cgatgctgga tgtcggcgcg aaaaaattcc
     1321 ggccgtccac gctacccgat gtctacgatg cggcgcgtct tgtcgacaag ctcgataaca
     1381 ttcacgcgtt ctcccgcctg attgtcgcaa ccgaaatgac cgggcgcttc gcctgcgacc
     1441 tgaatacggc gtatgtgagc gctgcgggta cggcgaagca caccgcgttg acctttggtg
     1501 acgccaggaa cgtcgagccg acgctcgaaa tgctgtacat gattgccggc ggcaaggaca
     1561 agttcctgga acggcccttc gcccatggtg gcggttgcgc catcgtttcg ccgctgcgct
     1621 acagccagga aaactgcgag gtctgcatcg agtcgaccaa gttcggggcg ccggtgtgga
     1681 tcgtgaccgc accgcaggcc ggggcgacgg cacccgccgc attggcgggg acgctggtgc
     1741 aggtcacggc cgaggcgctg gcctgcctgt tgatgatcaa cctggtggtg ccgaagtacc
     1801 cggtgatcat ggggccctgg ccgttcgttt ccgacctgcg caccggcgcg ttcaccgggg
     1861 gcggcggcga agaagccatc gtctcggcgg cggccgggca gatcatgaac tggtacgggc
     1921 tggtcagttc tgttggtgcc ggtatgaccg atgccaagtc gcccgataac caggcgggtt
     1981 acgagaaggc gatcgccgtg ttgctggcgg cacaggcggg caccaacaac gtgtcggagt
     2041 ccgcgggcat gatgggcagc ctgatggggc tgagttttga atcgctggtg atcgataacg
     2101 acatgctcgg cgcggtgatg cgcacggtgc gcggtatcga ggtaaacgag gaaacgctgt
     2161 cagtcgatat tattcgcagc gctattgcgg gcgaaggcca ctacctgcgc caggaccaga
     2221 ccttgtcgct gatgcgcacg gagtacgaat acccgaagct ggctgatcgg cgcacgccca
     2281 acgactgggt cgatgccggc tcacccgata ttcgtgaaca ggcggccgag cgcgtgaagg
     2341 aaatcctttc gacacactac cccgagtaca ttcacccgga cgtcgataaa aagattcgcg
     2401 agaagttcaa gatcgagctg ccgcgcgagt acatgttcgc ctccagcggc cgctggtagt
     2461 cagcgcgacg aatatcaggt cgtgacagca acctgccttt ttgcgcgcca gtcggcgatc
     2521 gcgaacggca ggtagaagat cgtgaacagt cccagcgcga ggaactccgc ccagaacagg
     2581 taccacggcc acggacccat catgtcgagg atgctggccg acgccggctt gcccagcgtg
     2641 tacatgtaat tgctgccaat cagcaggttc acgccggtga ccagcaccat gtagatgttg
     2701 gcggcgaata gcgctttcca gatggacctc cacgtcgggc gataaccttc gatgaccgtc
     2761 atgaaggtca tgacgatgat gatcatgccg tgggcggcga tggtctgaat ggcgcggaaa
     2821 tgcggcaggc cgtactcgcc ggcatcgggt gtcagcactg tctgcgtggc gcctgcgaga
     2881 ccgatgaaaa agatgatttc gtaaatccgg tagctgcgcg tgatcagcac caggatcgaa
     2941 caccaggcac tgatcgaaca ggcatgcagc ggcaggtgct tctgg
//
LOCUS       DATCZV010000139         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_327341, whole genome shotgun sequence.
ACCESSION   DATCZV010000139 DATCZV010000000
VERSION     DATCZV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327341"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VIW27_03060"
     CDS             <1..515
                     /locus_tag="VIW27_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002908202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5738675.1"
                     /translation="AGIQHVAPLEAFSDENWQRIIEINLSAPFRLIRRALPAMRANGW
                     GRIINIASVHGLVASRHKSAYVAAKHGLVGLTKTVALETAAEPITCNALCPGYVRTAL
                     IEAQIASLAAVENISPEQAAESILREKQPSNRFVAPADIGSMVTFLCSAASSQITGAA
                     LTMDGGWSAQ"
     gene            complement(834..1784)
                     /locus_tag="VIW27_03065"
     CDS             complement(834..1784)
                     /locus_tag="VIW27_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEY5738676.1"
                     /translation="MPDIVVMFFLLGLVAGLVRSDLSLPRAAYDTISLLLMLTIGLKG
                     GMALYGNLGWQLLPELLAIITLGASIPLLLFPLLRKVVRLGLADCASIAAHYGSVSAG
                     TFAVALAYIESRDIAYAPEVTLYLVAMELPAIVVGLLLYRRLQPSTPAQPSLNKLWHE
                     ALTNRSVILLLGGVIIGMMYGPQQGAAVTDLYTGAFKAVLALFLLEMGLTAAETLRPI
                     PWHRWRLIAFALLIPPVLAVAGIGIALLLELPPGSAIILASLAASASYIAAPVAIGSA
                     IKEANIGLAMLASLGLTFPFNVLIGIGLYHRLIDLIPAFS"
     gene            complement(1829..2194)
                     /locus_tag="VIW27_03070"
     CDS             complement(1829..2194)
                     /locus_tag="VIW27_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738677.1"
                     /translation="MKNIDGIYRVELLGPYGWERFSTAFIDGGEFRSASSEHFTAGSC
                     TADGEGFRMRGRMTRYVDNRALFGRSELRGLPIQFNGVIRDGIIDGEATVVDDARRSL
                     RFRLARLALPDGGGGRRTA"
BASE COUNT          486 a          782 c          834 g          423 t
ORIGIN      
        1 atgccggcat acaacatgtc gcgccgctcg aggcattcag cgacgaaaac tggcagcgca
       61 tcatcgagat caacctgtcg gcgccgttcc ggctcattcg ccgggcgctg ccggcgatgc
      121 gcgccaacgg ctgggggcgg attatcaata tcgcctcggt tcacggcctg gtcgccagcc
      181 ggcacaaatc agcttacgtg gccgccaagc acgggcttgt cggcctgacc aaaacggttg
      241 ccctggaaac ggcggccgag cccatcacct gcaacgcgct atgcccggga tacgtgcgca
      301 ccgcgctgat cgaagcgcag atcgccagcc tggccgcggt ggaaaacatt agccccgagc
      361 aggcggcgga atcgattctg cgggaaaagc agccctcgaa ccgcttcgtc gccccggccg
      421 atattggctc aatggtcacc tttctgtgtt cggcggcaag ctcgcagata accggtgctg
      481 cattgaccat ggacggtggc tggtcggcgc agtagggcgg taacccggcg cgagcctgag
      541 gcaacgttta cccgcggccg ccgggaagat gcggcggagt tacgaggtcg gtcgccggct
      601 gcggcccgaa ttcccccgcg ggagcatggc atggtcgcgg caaagcgcgc tgtgggcgtc
      661 gcttcgccgc tcgcacccgt tgcatcgccg gcaaaatcct cgctttcctc gctggcaaag
      721 tcgccgcgcc gccaggatat tgttagcgac aatcctcgcg ccctgcgcgg tacgggtcga
      781 cgctgcgaga ccggttagcg aaaaaccgat cgcggcgagc aatccggccc gcttcaggag
      841 aaggccggta tcaggtcgat cagtcggtga tacaggccga taccgatcaa cacgttgaag
      901 gggaaggtca ggcccagcga agccagcatg gcgaggccga tgttggcctc cttgatggcg
      961 ctgccgattg ccaccggggc ggcgatgtag gacgcgctcg cggcgaggct ggcaagaatg
     1021 attgccgatc ccggcggcag ttccagcagc agcgcgatgc cgatgccggc taccgccagc
     1081 actggcggga ttagcagcgc gaaagcgatc agccgccagc gatgccatgg aatcgggcgc
     1141 agggtttccg ccgcggtcag tcccatttcg agcaggaaca gggccagcac cgccttgaag
     1201 gcgccggtat acaggtcggt taccgccgcg ccctgctgcg ggccgtacat catgccgatg
     1261 atgacgccgc cgagcagcag gatcacgctg cgattggtca gcgcttcgtg ccacagcttg
     1321 ttcagcgaag gttgtgccgg cgtcgagggc tgcaggcgtc ggtagagcag caggccgacg
     1381 acgatcgccg gcagttccat tgccaccagg tagagtgtga cctccggcgc gtaggcgata
     1441 tcgcggcttt cgatgtaggc cagcgccacg gcgaaggttc cggcgctgac cgaaccgtag
     1501 tgagcggcta tgctggcgca gtcggcgaga ccgagccgca cgactttgcg gagcagcggg
     1561 aacagcaaca ggggtatgct ggcgcccagg gtgatgattg cgagcaactc cggcagcagc
     1621 tgccagccca ggttgccgta cagtgccatt ccgcccttga gcccgatagt cagcatcagc
     1681 agcaggctga tcgtatcgta ggcggccctg ggcaggctca ggtcggaacg gaccagcccg
     1741 gcgaccaggc cgagcaggaa aaacatcacc acgatatcag gcacgatatc acctccggtt
     1801 tctttcggtg cgtttcaagc cgctgcgctc aagctgtccg gcgaccgccg ccgccgtcgg
     1861 gcaaagccag cctggcgaga cgaaagcgca gcgacctgcg tgcgtcgtcg acgactgtcg
     1921 cttcgccgtc gataatgccg tcgcgaatca cgccattgaa ctggatcggc aatcccctga
     1981 gttcgctgcg gccgaacagc gcgcggttat cgacatagcg ggtcatgcgg ccgcgcatgc
     2041 ggaagccctc gccatcggcg gtacagctac cggcggtaaa atgttcgctg ctggcgctgc
     2101 gaaattcgcc gccgtcgata aacgcggtgg aaaatctttc ccagccgtag gggccgagca
     2161 gttctacgcg gtaaataccg tcgatattct tcatgggttg cctgcaagcg gttgccaaac
     2221 gcgaacggcg gctatcatag gggcccgtaa aaaattatta attcgaatta ttcggcaata
     2281 ttattcgaaa tattcgaatt ggggctggta tcggcgaagt cgatcgcgat gggcggccga
     2341 cgccgatcgc gatcgatgat ggccgttgac ggcaagccgc tatcgaccgc cgccgcgtgc
     2401 cggcagccct gatttttccc gggggccatc gaagtcgccc aaacagcggg gcgccctggc
     2461 gggcgggccg tggacagccg ggcttgcggg gtcggccggc agcagtgttc caaaaataaa
     2521 gaacc
//
LOCUS       DATCZV010000140         4430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_115516, whole genome shotgun sequence.
ACCESSION   DATCZV010000140 DATCZV010000000
VERSION     DATCZV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115516"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..329
                     /locus_tag="VIW27_03075"
     CDS             3..329
                     /locus_tag="VIW27_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013962647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY5738678.1"
                     /translation="MIDKFQLWAVIIALGLGTYLIRFSFIGMIGGRPLPEWLLRHLRY
                     VAVAVIPGLIAPLVVWPAATGGETDPARMIAALVAFAIGIGFNSVLGAVFGGMAGLYL
                     MQFLLG"
     gene            343..1272
                     /locus_tag="VIW27_03080"
     CDS             343..1272
                     /locus_tag="VIW27_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008562215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5738679.1"
                     /translation="MLVEILDVVAPVFIVVGAGYTAVRTGFFTAAHIDQLMKFAIQIA
                     VPCLLFRAASTIDLAAAFDWRMLLSYYAAAFVCFIVAFLVMRKVFRRRPGEAVAIAFG
                     ALFSNLVLLGLPISERAFGADSLAPNFALVSVNAPFCYLVGITAMEFLRADGRGAAET
                     TRIVLRAMFRNSIMIGIGLGFIVNLGEIALPEALIGAVDLLARASLPVALFALGGVLT
                     RYSLSKSLGEAGVIALLSLAVQPALVWLIATQLQVPYGITHSVVLMAAVAPGLNAYLF
                     ASMYDRGLGTAASSVLLATILSVFTVSAWLVIL"
     gene            1283..1999
                     /locus_tag="VIW27_03085"
     CDS             1283..1999
                     /locus_tag="VIW27_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY5738680.1"
                     /translation="MMIGGRDEVMRKALLDALSLPAFILFFTMMGFGSLMQSSGFDAL
                     MAAAATLLIWGLPGQLAMVELTTTGQGIVAIVFACSLANARFMPMVVSFIPAMSRGGA
                     SLWRLLLDAQLLSINSWAMCLREFPRVEPAYRHLYYVTFAGSIMSAAVIGTLAGFYGA
                     FFLPVAIVLGLIFTSPLFFALVLSSTDGLEERLSMLAGCAIVGLAHWLLPAVDLLVTG
                     FVGGSVGFALGRFWRRRGQG"
     gene            1986..2318
                     /locus_tag="VIW27_03090"
     CDS             1986..2318
                     /locus_tag="VIW27_03090"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY5738681.1"
                     /translation="MGKAEWLWLIVLGCGLATFVWRALGVLVVRRIDPEGAFFKWVAC
                     VSYAMVAGLIFRMLVMPESDLAQVSLPLRLAAVAIAFAAYFVCGRRLVAGVLAGGSSL
                     AAMAQWLG"
     gene            complement(2341..3195)
                     /locus_tag="VIW27_03095"
     CDS             complement(2341..3195)
                     /locus_tag="VIW27_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738682.1"
                     /translation="MTRNPISAPVLLAAFCVMCLESPLVIAQEQERSADEIAAELANP
                     NTPVASLNFKTQYRTFDGDLPDADRQSGTTILFQPSLPFPLDNGDQILFRPAIPLIVD
                     QPVFSGAEFDGESGIGDIVFDLAYAPKNDKGLLIAYGLVASLPTATEDGLGSERYTLG
                     PEYLIGKITKTYVIGAFPNHQWDIGGSGDQDISLTTIQLFGTILPGGGWNYGSSPIIT
                     YDWESEQATVPLNFNFGKTVIANGQPWKLSFELNYYVEQADAFGPEWMLGFNVTPVVE
                     NRLASWFD"
     gene            3441..>4430
                     /locus_tag="VIW27_03100"
     CDS             3441..>4430
                     /locus_tag="VIW27_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5738683.1"
                     /translation="MEIPLVRARYAHAFARTLDKLGVPTSRLLQAHRLPPELAERQDG
                     LIAAYQLWSFAGDAARREGLSELGLLAGRVPVVEHGEFGKMVYQALTLYDAIRTFCDN
                     ARAEYSRADIYLTRDRNQAWFCRGPIDGDSAIQRQQVELYVLIMMIDTIRLGTGSDWV
                     PTRIHLQNEDRRGLADIPLFRNADTRFGCAATAVGIPLEALGNPLQGDISAFAKNTAD
                     PDDSGILDFDFANSLRRLLQNYLGSMPLTLDVTAEITGITPRTLQRRLSESGMTYQQV
                     IEQLRFETALPMLLDQGLNITAIAFELGYSDSAHFTRAFRRWAGMSPSEYRRRH"
BASE COUNT          692 a         1306 c         1438 g          994 t
ORIGIN      
        1 gcatgatcga caaatttcag ctctgggctg ttatcattgc gctcgggctc ggcacctacc
       61 tgatccgctt ttcgtttatc ggcatgatcg gcgggcggcc gctgcccgaa tggttgctgc
      121 gccacctgcg ctatgtcgcg gtcgcggtga taccggggct gattgcgccg ctggtggtct
      181 ggccggccgc taccggcggt gaaaccgatc cggcgcgaat gatcgccgct ctggtcgcct
      241 tcgccatcgg tatcggtttc aacagcgtgc tcggcgcggt gttcggtggc atggcggggc
      301 tttacctgat gcaatttctt ctgggctgac agcaggatat caatgctggt cgaaattctc
      361 gatgtcgtgg cgccggtatt catcgtcgtc ggtgccggtt atacggcggt aagaaccggc
      421 tttttcaccg ccgcgcatat tgaccagttg atgaagttcg cgatccagat cgcggtgccc
      481 tgcctgttgt tccgcgccgc gtcgacgatc gacctggcgg cggccttcga ctggcgcatg
      541 ctgctgtctt attacgctgc cgcgttcgtc tgtttcatcg tcgccttcct cgtcatgcgc
      601 aaggttttcc gccgtcgtcc cggcgaagcg gtggcgatcg ctttcggcgc gctgttttcc
      661 aacctggtgc tgctcggcct gccgatctcg gagcgcgcct tcggcgccga cagcctggcg
      721 ccgaatttcg cgctggtgtc ggtcaatgcg ccgttctgtt acctggtcgg catcaccgcg
      781 atggaattcc tgcgcgccga cggtcgggga gcggcggaaa ccacgcgtat cgtgctgcgg
      841 gcgatgtttc gcaacagcat catgatcggc atcgggctgg gtttcatcgt caatctcgga
      901 gagatcgcgc tgccggaggc gctgatcggc gccgtcgacc tgctggcgcg cgcctcgctg
      961 ccggtggcgc tgttcgcgct cggcggcgtg ctgacccgct acagcctgtc gaaatcgctc
     1021 ggtgaggccg gcgtgatcgc gctgctctcg ctcgccgttc agccggcact ggtctggctg
     1081 atcgccacgc aactgcaggt accctatggc atcacgcatt cggtggtatt gatggcggcg
     1141 gtggcgccgg ggctcaatgc ctatctgttc gccagcatgt acgaccgcgg gctcggcacc
     1201 gcggccagct cggtattgct ggcgacgatc ctgtcggtat tcacggtcag cgcctggctg
     1261 gtgatcctgt gatccacgga cgatgatgat cggcggccgc gacgaggtga tgcgcaaggc
     1321 gctgctcgat gcgttgtcgc tgccggcctt catcctgttt ttcacgatga tgggcttcgg
     1381 ctcgctgatg caaagcagcg gtttcgatgc gctgatggcc gccgcggcca cgctgctgat
     1441 ctggggtctg ccgggacagc tggcgatggt cgagttgacc acgaccggac agggaatcgt
     1501 cgccatcgtc ttcgcctgtt cgctcgccaa tgcgcgtttc atgccgatgg tggtctcttt
     1561 cattccggcg atgtcgcgcg gcggcgcgag cttgtggcgg ctgctgctcg atgcgcaact
     1621 gctgtcgatc aattcctggg cgatgtgcct gcgcgaattt ccgcgcgtcg aaccggctta
     1681 ccggcatctt tactatgtga ctttcgccgg ctcgatcatg agcgcggcgg tgatcggcac
     1741 tttggccggc ttttacggcg cgtttttcct gccggtggcg atcgtgctcg gactgatatt
     1801 taccagcccg ttgtttttcg cgctggtgct gtcgtcgacc gacggactcg aagagcgcct
     1861 gtcgatgctc gccggctgcg cgatcgtcgg ccttgctcac tggctgctgc ctgccgtgga
     1921 cctgctggtc accggcttcg tcggcggcag cgtcggcttc gccctcggac gtttctggag
     1981 gcgccgtggg caaggctgag tggctttggc tgatcgttct cggctgcggt ctcgcgacct
     2041 tcgtctggcg cgctctcggc gtgctggtgg tgcgccgcat cgatcccgag ggggcctttt
     2101 tcaaatgggt tgcctgcgtc tcctacgcga tggtcgcggg cctgattttt cgcatgctgg
     2161 tgatgccgga aagcgatctt gcgcaggtga gcctgccgct gcggctggcc gcggtggcga
     2221 ttgccttcgc ggcctacttc gtctgcggcc gccggctcgt cgccggtgtg ctcgccggcg
     2281 gttcgagcct tgccgcgatg gcgcagtggc tgggctgatc gttaaacgcg ttcggccggg
     2341 ctagtcgaac cagcttgcca gcctgttctc gaccaccggc gtaacgttga agccgagcat
     2401 ccattcgggg ccgaatgcgt cggcctgctc gacgtagtaa ttcagttcga aactcagctt
     2461 ccagggctgg ccgttggcga tcaccgtctt gccgaagttg aagttgagcg gcacggtggc
     2521 ctgttcgctc tcccagtcgt aagtgatgat cggcgaggaa ccatagttcc agccgccgcc
     2581 gggcaggatg gtgccgaaca actgtatcgt ggtaaggctg atgtcctggt cgccggagcc
     2641 gccgatatcc cactggtgat tcgggaaagc gccgatgacg taggttttgg tgatcttgcc
     2701 gatcaggtac tccgggccca gggtgtagcg ctccgagccg aggccgtcct cggtcgcggt
     2761 cggcagcgat gcgaccagcc cgtaggcgat cagcaggccc ttgtcgtttt tgggcgcgta
     2821 agcgagatcg aagacgatat ccccgatgcc ggattcgccg tcgaattccg ctccagaaaa
     2881 aaccggctgg tcgacaatca gcggtatcgc cggacggaac aggatctggt cgccgttatc
     2941 gagcgggaac ggcaggctcg gttgaaacag gatcgtcgtg ccggattgcc ggtcggcatc
     3001 gggcagatcg ccgtcgaaag ttcggtactg agttttgaag ttgaggctcg ccaccggggt
     3061 attgggatta gccagttccg cggcgatttc atccgcgctg cgctcctgct cctgcgcgat
     3121 gaccagcgga gactccaggc acatcacgca gaatgcagcc agcaagacgg gggccgagat
     3181 ggggttacgg gtcattagtc atccagaatc tggttcttcg gaagggcaga ttgctacagg
     3241 actacaggga ctgtcctgcc atttgacgac aatcgtcact gttatgactg tccgaatgaa
     3301 tggcgagtta gccgataggc tgtcagattt cgcttatttg gagagatggc ggcaaatggt
     3361 aacttggcgt taaatggcaa ttattgtccg gtgcggtgta gtatgtttta gaccctcagt
     3421 cattccattg ggttgattag atggagatac cgctcgtcag ggcccgatac gcgcacgcat
     3481 ttgccaggac actggacaag ctcggtgtgc cgacaagccg gctgctgcag gcgcaccgcc
     3541 tgccgccgga gctggcagaa aggcaggacg gcctgattgc cgcgtatcag ctctggtcgt
     3601 ttgccggcga tgccgcgcgc cgcgagggcc tgtccgaact gggcttgctg gccggccgcg
     3661 ttccggtggt ggagcacggt gaattcggca agatggttta ccaggctctg acgctgtatg
     3721 acgctattcg gacgttctgc gacaacgcaa gagccgagta ctcgcgggcc gacatttact
     3781 taacccggga tcggaaccag gcatggtttt gccgcggccc catcgatggc gactctgcaa
     3841 tacagcgcca gcaggtcgaa ttgtatgtac tgatcatgat gatcgatacg attcgccttg
     3901 gaaccggcag cgactgggta ccaaccagga ttcacctgca aaacgaagat cgcagggggt
     3961 tggccgatat cccgctgttt cgaaatgccg atacgcgttt cggctgcgcg gccaccgcgg
     4021 tcggcattcc cctcgaggcg ctcggtaatc ccctgcaggg cgatatttcg gcgttcgcca
     4081 aaaacacggc ggatccggat gacagcggga ttctcgattt cgactttgcc aattccctgc
     4141 gccggctact gcaaaattac ctcggcagca tgccgctgac gctcgatgtg acggcggaaa
     4201 tcaccgggat tacgccgcga accctgcagc gcaggttgag cgaatcgggg atgacttatc
     4261 agcaggtcat cgaacaactg cgcttcgaaa cggcgctacc catgctgttg gaccaggggc
     4321 tcaacataac ggcaatcgcc ttcgaactgg gctattccga ttcggcgcat ttcacccgtg
     4381 cgtttcgccg ctgggccggt atgagcccca gcgagtaccg gcgccgtcac
//
LOCUS       DATCZV010000141         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_380546, whole genome shotgun sequence.
ACCESSION   DATCZV010000141 DATCZV010000000
VERSION     DATCZV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380546"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="lpxD"
                     /locus_tag="VIW27_03105"
     CDS             <1..887
                     /gene="lpxD"
                     /locus_tag="VIW27_03105"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY5738684.1"
                     /translation="QDIAASDCAAVLVPAELAAEIDDRATLIADDPQLAFVQAIELLQ
                     VEGEPPPAGIHPSAQVAASASLGDDVAIDALAVIGEGAVIGRGTRIGAGCVIGDEVEV
                     GDDCRLHARVALSHGVVLGNRCILHPGAVIGADGFGLVVEDERWRKVPQLGGVRIGDD
                     VEIGANTTVDRGALDDTVIEKGCKLDNLIQVAHNVRIGAHTAIAACVGIAGSAEIGRY
                     CKISGAAVILGHLRIADRVTVTAMSLVTRDIPEAGVYSSGTPLMENRQWHRSNARYKQ
                     LEALARRIAALEKFADQQ"
     gene            944..1363
                     /gene="fabZ"
                     /locus_tag="VIW27_03110"
     CDS             944..1363
                     /gene="fabZ"
                     /locus_tag="VIW27_03110"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012325876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY5738685.1"
                     /translation="MELLPHRYPFLLVDRVVGLEPGKSLSAIKNVSINEPFFQGHFPG
                     QPIMPGVLILEAMAQATGLLAFSSMGDAHKSKLYMLVGIDKARFRGQVVPGDQLALNI
                     SLKRNMRGIGMFGCEALVDGEVVAEAEMMCSAQDRQQ"
     gene            1360..2130
                     /gene="lpxA"
                     /locus_tag="VIW27_03115"
     CDS             1360..2130
                     /gene="lpxA"
                     /locus_tag="VIW27_03115"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY5738686.1"
                     /translation="MIHATAIVDPGARLAGDVEVGAYSIIGEGVEIGAGTVVGPHVVI
                     MGPTSIGRENRIFQFSSIGEAPQDKKYGGEPTRLELGDRNVIREFVTLNRGTDEGNGK
                     TVIGSDNLFMAYTHVAHDCIVGNHTIFANAASLSGHVEVGDHAILGGFTSVHQFTQIG
                     SRAFCGLGSVITQDIPPFTTVAGNRARVVGINKEGLRRRGFSPELIRALHKSFRELLK
                     SRVSKPQAFENLKPLCDKYPEVEEFVSFVRNSKRGIAR"
     gene            2240..>2690
                     /locus_tag="VIW27_03120"
     CDS             2240..>2690
                     /locus_tag="VIW27_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738687.1"
                     /translation="MQLAGLAEGVQGQDILHFIEAPEYAEVLEDARGLKLTDDGTVYV
                     TSEQRGQLLKIVDGKIEQHSLVPSAFEDEDLGGIDVLPDGRLVIVNEGSGKVGVLNSG
                     LEPEILFSSSGGSVGELDDPLAVAVSVNGNIFVGDVKNRQVSVFNHQG"
BASE COUNT          528 a          794 c          862 g          506 t
ORIGIN      
        1 tgcaggatat cgccgccagc gactgtgccg cggtgctggt acccgccgaa ctggcggctg
       61 aaatcgacga ccgcgcgacg ctgatcgccg acgatccgca gctcgccttc gtgcaggcaa
      121 tcgagctgct gcaagtcgaa ggcgagccgc cgccagccgg catccacccg tcggcgcagg
      181 ttgccgccag cgcgagcctg ggtgacgatg tcgcgatcga cgccctggcg gtgatcggcg
      241 agggtgccgt gatcggccgt ggaacccgga tcggcgctgg ttgcgtgatc ggcgacgaag
      301 tcgaggtcgg cgatgactgc cgtctgcacg cccgcgtcgc gctgtcgcac ggcgtggtgc
      361 tcggcaatcg ctgcattctg cacccgggag cggtgatcgg cgccgacggt ttcggcctgg
      421 tagtcgagga cgagcgctgg cgcaaggtgc cgcagctcgg cggcgtgcgc atcggcgacg
      481 atgtcgaaat cggcgccaat accaccgtcg accgcggcgc actcgacgat accgtgatcg
      541 agaagggctg caagctcgat aacctgatac aggtcgcgca taacgtgcgt atcggcgcgc
      601 ataccgcgat cgccgcctgt gtcggtatcg ccggtagcgc cgagatcggc cgctactgta
      661 aaatttccgg cgccgcggtg atactcggcc acctgcgcat cgccgaccgg gtcacggtca
      721 ccgcgatgtc actggtcacc cgcgatattc ctgaagccgg tgtctactcg tcgggaacgc
      781 cgctgatgga gaaccgccag tggcatcgca gcaatgcgcg ttacaaacag ttggaagcgc
      841 tggcgcggcg tatcgcggct ctggaaaaat tcgccgacca gcaatgatcg gctcaatcga
      901 aactgacaag gggtaaaatt tggaactcga tatcagcaag gtgatggaat tgttgccgca
      961 tcgctaccca ttcctgctag tcgaccgtgt agtcgggctc gagccgggca agagcctgtc
     1021 ggcgataaag aatgtgtcga tcaacgagcc cttcttccag gggcatttcc ccggacaacc
     1081 gatcatgccg ggcgtattga tactcgaggc gatggcacag gccaccggcc tgctggcatt
     1141 cagttccatg ggcgacgccc acaagtcgaa gctctacatg ttggtcggca tcgacaaggc
     1201 gcgctttcgc ggtcaggtcg tgcccgggga ccagctggca ctgaatattt cgctgaaacg
     1261 caacatgcgt ggtatcggca tgttcggctg cgaggcactg gtcgatggtg aagtcgtcgc
     1321 cgaggccgaa atgatgtgct cggcgcagga tcgtcagcaa tgatccatgc gactgccatc
     1381 gtcgatccgg gtgccaggct tgccggcgac gtcgaagtcg gcgcctacag catcatcggc
     1441 gaaggcgtgg agatcggcgc cggtaccgtc gtcgggccgc acgtggtgat catgggtccg
     1501 acgtcgatcg ggcgggaaaa tcgaatattc cagttttcgt cgatcggcga ggcgccgcag
     1561 gacaagaaat acggaggcga gccgacccgc ctcgagctcg gcgaccgcaa cgtgattcgc
     1621 gaattcgtca ccctcaatcg gggcaccgac gagggcaacg gcaagacggt gatcggcagt
     1681 gacaacctgt tcatggccta tacccacgtg gctcacgatt gcatcgtcgg caaccacacc
     1741 atattcgcca acgccgcttc gttgtcgggc catgtcgaag tcggcgacca tgccattctc
     1801 ggcggtttca cctcggtaca ccagttcacg cagatcggca gccgggcttt ctgcggcctc
     1861 ggatcggtga ttacccagga tataccgccg tttaccacgg ttgccggtaa tcgtgcccgg
     1921 gtggtcggaa tcaacaagga aggcctgcga cgccgcggtt tttctcccga attgatacgt
     1981 gctttgcata aatcgttccg agaactctta aaatcgaggg tttcgaaacc acaggcattt
     2041 gaaaatctca agcctttatg tgataaatac cccgaagtgg aggaatttgt cagcttcgtg
     2101 cgcaacagta agcgaggtat cgcccgctag cggggttgaa gcacgctgca cgtttcggac
     2161 agccgaatcc gtcgctcatg gatacacggg gaggtgtcag atcaaaccgc gttcgaggtt
     2221 cctgtcgaca ctgcttgcaa tgcagctcgc cggccttgcc gaaggcgtgc aggggcagga
     2281 tatcctgcat ttcatcgagg cgccggaata cgccgaggtg ctggaggatg cccgcggact
     2341 gaagctgacc gacgacggca ccgtctatgt caccagcgag cagcgcggtc agctgctgaa
     2401 aatcgtcgac ggcaagatcg agcagcattc gctggtgccg tcggcattcg aagacgagga
     2461 cctcggcggc atcgatgttt tgccggacgg ccgcctggtg attgtcaacg agggctcggg
     2521 aaaggtcggc gtgctgaatt cggggctcga acccgaaatc ctgttctcga gttccggcgg
     2581 atcggtcggt gaactcgatg atccgctggc ggtagcggtc tcggtcaacg gcaatatctt
     2641 cgtcggcgac gtcaagaacc gccaggtcag cgtcttcaac caccagggtc
//
LOCUS       DATCZV010000142         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_62739, whole genome shotgun sequence.
ACCESSION   DATCZV010000142 DATCZV010000000
VERSION     DATCZV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62739"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIW27_03125"
     CDS             <1..286
                     /locus_tag="VIW27_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEY5738688.1"
                     /translation="HRVGGLYSDDWHRVHLISPRDVVPESIMPGYPWLEGEPLDDSDI
                     QAKMRTLRRLGVPYSDEQIESAPQALEGKTEMDALIAYLQGLGTQIKSRR"
     gene            289..459
                     /locus_tag="VIW27_03130"
     CDS             289..459
                     /locus_tag="VIW27_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY5738689.1"
                     /translation="MSITFVQSIWTIVVMVTFLGIVAWAFSSKRKASFDEAARLPFDD
                     EPHGNRRSSDPE"
     gene            464..1369
                     /gene="ccoP"
                     /locus_tag="VIW27_03135"
     CDS             464..1369
                     /gene="ccoP"
                     /locus_tag="VIW27_03135"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY5738690.1"
                     /translation="MADFNSDFWHWYIAIATIISILACVWLVRWMTSGFEKKDQVENT
                     GHVWDGDLTELNNPLPRWWLGLFYLTLAFGGFYLLLYPGLGSFQGLLGWSSKGAYEQE
                     VETIEAEVRPLFERYSQTPVLDLIEDEDALKVGERLFLTYCSTCHGSDARGARGYPNL
                     RDRAWLWGGDPQSIKTSITNGRVAAMPAWLAPLGGEAGVDEVTQYVLSLSGRPAIEEM
                     AAKGKLKYQTFCVACHGPEGKGNPALGAPNLTDGNWLYGGSITRIAESIANGRNGQMP
                     AQAERLGEAKIHLLTAYVYSLSQQR"
     gene            1369..1644
                     /locus_tag="VIW27_03140"
     CDS             1369..1644
                     /locus_tag="VIW27_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738691.1"
                     /translation="MDPVAPQKLPRTQVAIAVLWPSFLVAIAATGVFFSAFNPRELIP
                     FNLDIEVSPLAAYSIGFFCFWLIGILAGYGTVYFTVTNARLSGPPND"
     gene            1667..>2667
                     /gene="ccoG"
                     /locus_tag="VIW27_03145"
     CDS             1667..>2667
                     /gene="ccoG"
                     /locus_tag="VIW27_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY5738692.1"
                     /translation="MPDASQHTESQEFYAAHQKIYPREVEGRFARLRLLSFIVLLGIY
                     YALPLLRWDDRQAVLFDLPARKFYIFGLTLWPQDFIYLAALLILAGLSLFFFTALFGR
                     VWCGYACPQTVWTEVYLWMERKIEGNRSQRMKLDKAPASAAKIRTKALKHTIWILFSL
                     WTGFTFVGYFTPIDVLANKLVMLSLGPWETFWILFYSFATYGNAGFMREQVCLYMCPY
                     ARFQSAMFDSNTLIVAYHPERGEPRGSRRRSADPAQLGLGDCIDCSICVQVCPTGIDI
                     RNGLQYECIACSACIDACDEVMDKMSYPRGLIRYTTENSALGQRERILRPRILVYAA"
BASE COUNT          475 a          878 c          802 g          512 t
ORIGIN      
        1 gcaccgggtt ggcggcctgt acagcgacga ctggcatcgc gtgcacctga tcagcccgcg
       61 cgacgtggta cccgagtcga tcatgccggg ctatccgtgg ctcgaggggg aaccgctcga
      121 cgacagcgat atccaggcca agatgagaac cctgcgccgc ctcggcgttc cctatagcga
      181 cgagcagatc gagagcgcgc cgcaggcgct cgagggcaag accgaaatgg atgcgttgat
      241 cgcttacctg cagggactcg gcacgcagat caagtcgagg agatgagcat gtcgatcacc
      301 tttgtgcaat ccatctggac catcgtcgtg atggtgacct tcctcggtat cgtcgcctgg
      361 gcgttcagca gcaagcgtaa ggcgagcttc gacgaggccg ctcgcctgcc gttcgacgac
      421 gaacctcacg gcaaccgccg ctcttccgac ccggagtaga ttcatggccg acttcaacag
      481 cgacttctgg cactggtaca tcgccatcgc aacgatcatc agcatcctcg cctgcgtctg
      541 gctggtgcgc tggatgactt cgggcttcga gaaaaaggac caggtcgaaa acaccggcca
      601 cgtctgggat ggcgacctga ccgaattgaa caatccgctg ccgcgctggt ggctggggct
      661 tttttacctg acgctcgcgt tcggcggctt ctacctgttg ctgtatcccg ggctcggctc
      721 cttccagggc ctgctcggct ggtcttcgaa gggcgcatac gagcaggaag tcgaaaccat
      781 cgaagctgag gtcagaccgt tattcgagcg ctacagtcag accccggtac tcgacctgat
      841 cgaggatgag gacgcactca aggtcggcga gcgcctgttc ctgacctatt gctcgacctg
      901 ccacggctcc gatgcccgcg gcgcgcgcgg ctacccgaac ctgcgcgacc gtgcctggct
      961 gtggggcggc gatccgcaga gcatcaagac ctcgatcacc aacggccgtg tcgccgcgat
     1021 gccggcatgg ctggctccgc tgggtggcga ggccggcgtc gacgaagtaa cccagtacgt
     1081 gctgagcctg tccgggcggc cggcaatcga ggagatggcg gcaaagggca agctcaagta
     1141 ccagactttt tgtgtcgcct gccacggccc cgagggtaaa ggcaacccgg cgctcggcgc
     1201 gcccaacctg accgatggca actggctgta cggcggttcc atcacgcgca tcgccgaatc
     1261 catcgccaat ggccgcaacg ggcaaatgcc ggcccaggcg gagcgcctcg gcgaagccaa
     1321 gatccacctg ctgacggctt acgtctacag cctgtcgcag caacggtaat ggacccggta
     1381 gcgccgcaga aattgccacg cacgcaggtg gcgatcgcgg tgctgtggcc atcgttcctg
     1441 gtcgcgattg ccgccaccgg cgtgttcttt tcggccttca acccgcgcga gctgataccc
     1501 ttcaatctgg atatcgaggt cagcccgctg gccgcctaca gcatcggctt cttctgtttc
     1561 tggctgatcg gtatcctggc tggctacggg acggtatact tcaccgttac caacgcgcgc
     1621 ctttcggggc ccccgaacga ctaacagcgc tcacggcgcc ttcgacatgc ccgacgcaag
     1681 ccagcatacc gagtcccagg agttctacgc cgcgcaccag aaaatctatc cgcgcgaggt
     1741 cgaaggccgt ttcgcgcgcc tgcgcctgct atccttcatc gtcctgctcg gcatttatta
     1801 tgcactgccg ctgctgcgct gggacgaccg ccaggcggtg ctgttcgacc tgccggcgcg
     1861 caagttctac atcttcggcc tgaccctgtg gccgcaggat ttcatttacc tggccgcgct
     1921 gctgatcctg gccggcctgt cgctgttctt tttcaccgcg ctgttcggcc gggtctggtg
     1981 cgggtacgcc tgcccgcaga ccgtctggac cgaggtctac ctgtggatgg agcgcaagat
     2041 cgagggcaac cgctcgcagc gcatgaagct cgacaaggca cccgcgtcgg ccgcgaagat
     2101 tcggaccaag gcgctgaagc acaccatctg gatcctgttc tcgctgtgga cgggctttac
     2161 cttcgtcggt tacttcacgc ccatcgacgt actcgccaac aagctcgtca tgctgagcct
     2221 cggcccgtgg gaaaccttct ggatcctgtt ctacagtttc gccacctatg gcaatgccgg
     2281 cttcatgcgc gagcaggtct gcctgtatat gtgcccctac gcgcgctttc agagcgcgat
     2341 gttcgactcg aatacgctga tcgttgccta tcatccggaa cgcggcgagc cccgcggctc
     2401 gcgccgccgc agcgcggatc ccgcgcaact gggtctcggc gactgcatcg attgcagcat
     2461 ctgcgtgcag gtctgcccga ctggcatcga catacgcaac ggtctgcaat acgaatgcat
     2521 cgcctgttcg gcctgtatcg acgcctgcga cgaggtcatg gacaagatgt cctacccgcg
     2581 cggactgatc cgctatacca ccgaaaactc ggcgctcggc cagcgggagc gcatattacg
     2641 cccgcgaatc ctggtttacg ccgccat
//
LOCUS       DATCZV010000143         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_380849, whole genome shotgun sequence.
ACCESSION   DATCZV010000143 DATCZV010000000
VERSION     DATCZV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380849"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VIW27_03150"
     CDS             complement(<1..925)
                     /locus_tag="VIW27_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5738693.1"
                     /translation="MQRCDFIVSGARVVDGTGGISRNIDVAIDGGRIAALGDGNGWHA
                     EARIVADGLVLAPGFIDSHTHDDQAVFVSPLLECKLSQGVTTVIAGNCGISLAPLQAD
                     GTMPPPFPLLGDRQDFRFPSVADYRAALAAAPAAVNLALLAGHSSLRLGCMAERLHRE
                     ADAAAIAQMQQELRRALQQGCIGFSTGLTYPPAEAASTDEIVQLAGVLREFDKAVYAT
                     HMRDEGDRVVEAVAETLAIGSRARVPVVISHHKCAGANNYGRSSETLAMIDRARDAQQ
                     AVSLDVYPYTASSTSLLPQFVREAESVLVAES"
     gene            complement(946..1719)
                     /locus_tag="VIW27_03155"
     CDS             complement(946..1719)
                     /locus_tag="VIW27_03155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5738694.1"
                     /translation="MVKFLADIGRFNAVAIITLVLAAVSVMVTAVALAILEWVGLATI
                     SWLALSLAATVPLLAAPPIVWSLVDLLIDSFRKEQEMRQLASFDSLTGLPSRHAFFES
                     ANRHVSLANRNQSIFVVVLIDLDHFKAINDQYGHPAGDAVLRLFADVLNSVARRSDII
                     GRMGGEEFAMILPSTTTASALEFSERLHAAIGQAVLKYGESVIQYTVSIGIACSTAGR
                     VNNLENLLTRADLALYQAKRGGRNQTRVFTESQERIAVG"
     gene            1890..>2103
                     /locus_tag="VIW27_03160"
     CDS             1890..>2103
                     /locus_tag="VIW27_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY5738695.1"
                     /translation="MKVMMLIAHGSRKSAANDEVRQLAERIADIGGGDYDAVRAAFLE
                     LAEPDIQQGIDECVAQGATEIVAVPYF"
BASE COUNT          395 a          652 c          681 g          375 t
ORIGIN      
        1 tcgattcggc gaccagcacg gattcggcct cgcgcacgaa ctgcggcagc agcgaggtcg
       61 agctcgcggt ataggggtag acgtcgagac tcaccgcctg ctgcgcgtcg cgcgcccggt
      121 cgatcatcgc cagggtttcg ctggagcgcc cgtaattgtt ggcgccggcg cacttgtgat
      181 gagaaatgac gaccggcacc ctggcccgcg agccgatggc gagcgtctcg gccaccgcct
      241 cgaccacccg atctccctcg tctcgcatat gggtggcgta aaccgccttg tcgaactcgc
      301 gcagtacgcc ggccagttgt acgatttcgt cggtggacgc ggcctcggcg ggcggatagg
      361 tcaggccagt gctgaagccg atacagccct gctgcaatgc gcggcgcagt tcctgctgca
      421 tttgcgcgat tgccgcggcg tccgcttcgc gatgcagccg ctcggccatg cagccgaggc
      481 gcaggctgct gtggccggcg agcagcgcca ggttgaccgc tgccggtgcc gcggcgagcg
      541 ccgcgcggta atcggcgacc gatggaaaac gaaagtcctg ccggtcgccg agcagcggga
      601 aaggcggcgg catcgtgccg tcggcctgca atggcgccag gctgatcccg cagttgccgg
      661 caatcaccgt ggtgactccc tgggagagct tgcattcgag cagcggcgag acgaataccg
      721 cctggtcgtc gtgagtgtga ctgtcgatga aaccgggggc caggacgagg ccgtcggcaa
      781 cgattcgcgc ctccgcgtgc cacccgttgc cgtcgccgag agcggcgatc cgcccgccat
      841 cgatggcgac gtcgatgttt cgggaaatgc cgcccgtgcc gtcgacgacc cgggcgccgg
      901 aaacgatgaa atcacagcgt tgcatgttcc aaccccggcc ggtcttcagc caacagcaat
      961 gcgttcctgg gactcggtga atactcgtgt ctggttgcgg ccgccgcgct tggcctggta
     1021 gagggcgagg tcggcacgag tcaacaggtt ttcgagatta ttgacgcggc ccgcggtcga
     1081 acaggcgatg ccgatactga cggtgtattg aatcaccgat tcgccgtact tgagcactgc
     1141 ctggccgata gccgcgtgca gccgttcgga aaactccagc gcgctggcgg tagtcgtgct
     1201 cggcaggatc attgcgaatt cctcgccgcc catgcgaccg atgatgtcac tgcggcgggc
     1261 gacgctgttg agcacgtcgg cgaacagcct gaggaccgcg tcgccggcag ggtgcccgta
     1321 ctggtcgttg atcgccttga aatggtcgag atcgatgagt acgaccacga atatcgactg
     1381 attgcgattg gcgagagata cgtgacggtt ggcggattcg aaaaaagcat ggcggctggg
     1441 caagccggtc agcgaatcga agctcgccaa ctgccgcatc tcctgttcct tgcgaaaact
     1501 gtcgatcagc aaatcgacca gcgaccagac gatcggcggc gccgcgagca acggaacggt
     1561 ggccgccagc gacagcgcca gccacgaaat cgtcgccaac ccgacccatt ccagtatcgc
     1621 aagcgcgacc gcggtgacca tcaccgatac cgctgcgagc accagggtaa tgatcgcgac
     1681 cgcgttgaaa cgcccgatat ctgccaggaa tttaaccatg attacgtgcc gcccagatcg
     1741 cctgttatta ttgtgttttt gattttgcgg gccactttaa tgccatatcg gcccgggttc
     1801 aagtgattgc gggagtgccg gcgaatccaa attcccgcca ttcgcgtaca atttatcttc
     1861 tacgagagga accgggagac ccaaccacga tgaaagtgat gatgctgatc gctcacggca
     1921 gccgcaaatc cgccgccaac gacgaggtgc gccagctggc ggaacggatc gccgatatcg
     1981 gtggcggcga ctacgatgcc gtgcgggcgg cgttcctcga actcgccgag cccgacattc
     2041 agcagggtat cgacgaatgc gtcgcgcagg gcgccaccga gattgtcgcg gtgccctatt
     2101 ttc
//
LOCUS       DATCZV010000144         6457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_62826, whole genome shotgun sequence.
ACCESSION   DATCZV010000144 DATCZV010000000
VERSION     DATCZV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62826"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..942)
                     /locus_tag="VIW27_03165"
     CDS             complement(55..942)
                     /locus_tag="VIW27_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5738696.1"
                     /translation="MTVSPSITRRGNLWVGGFRCMASPCEVLLECDDRAQAHELIEIA
                     SREAFRIEQKFSRYRDDNIVHRINRGGQRLEVDEETARLLDFADNCYQLSDGWFDITS
                     GILRRAWKFGDGARLPRQREIDRLLAHIGWHQVSWEKPYLTLPAGMEIDFGGIGKEYA
                     VDSALGLIRDAADCSALVNFGGDCHASGPQAGGRPWMTGIENPNRPGDASAVIRLRQG
                     ALATSGDVFKYIEHRGRRYSHILNPKTGWPSPDAPRSVTVAAATCTEAGILSTLAMLQ
                     GGDAETFLDAQGVRYWCYR"
     gene            complement(1027..2274)
                     /locus_tag="VIW27_03170"
     CDS             complement(1027..2274)
                     /locus_tag="VIW27_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006992781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEY5738697.1"
                     /translation="MQLSKKKIAGLLSAATCSLLGGQSQAAGEWEVDTSILYYSEADD
                     RIQAIEPVIAARKDLGDDEILSWKLVVDSLTGSSASGAVPSTSPQTFTRPSGNGSYVV
                     EANQTPLDPSFKDTRVAYSMNWDKPLDRDRRRNLGFNLSREYDFTSLAVNGLWSLDSN
                     QKNTTWSFGSNLEYDVIDPVGGAPEPLTPMDDQRKQSGSEDRTIVDLLFGVTQVIDRS
                     SLFQVNLSLSLADGYLTDPYKLVSVVDNVSGEPIDQLFENRPDSRLRSALFAKYRKML
                     PGKDIFTASYRYMTDDWGVDSNTVDLTYRFRLGERYYLQPHLRYYQQSAADFYRYFLV
                     DGDLLPAEASADYRLGEMDATTVGVKFGHDIDGRHQWSVRLEQYVQSGESSPSEAFGQ
                     LAQQDLYPDVEATIVQFNYSFVF"
     gene            complement(2265..2486)
                     /locus_tag="VIW27_03175"
     CDS             complement(2265..2486)
                     /locus_tag="VIW27_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEY5738698.1"
                     /translation="MIARTRWLLIAALLLTAGCSSLGVKPWERDLLARDDMLMNADAL
                     DLAYDEHIYFSKEASSGGQGFAGGGCGCN"
     gene            complement(2483..2923)
                     /locus_tag="VIW27_03180"
     CDS             complement(2483..2923)
                     /locus_tag="VIW27_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012144153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5738699.1"
                     /translation="MKKLFLLLLLVAATPLRAEIFPIGDLDLKAHRGKVVYLDFWASW
                     CKPCRESFPWMNALLVKYPAENFTVITINLDAETAAMHRFLDKVPANFDIYHDPSGRL
                     AEQFQIEGMPTSYLISPTGEVVKKHIGFYTKHVERYEREIEELL"
     gene            complement(2999..4270)
                     /locus_tag="VIW27_03185"
     CDS             complement(2999..4270)
                     /locus_tag="VIW27_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5738700.1"
                     /translation="MKALLGDTPLFRKITASILLASVFFLSALSAIVFYSNEKLENDL
                     LDSQTRFELENALELLAQDPQSPLPAAASLSVYLHSRRATRPIPRHLVNLDPGVHHDL
                     VLDGKAYHVLVEPYGDDRIYIQYDVTEIERSEHLMQVIVVVAWVVLLVLIFFIARILS
                     RRISGPIAQLANELSHIDPDQRGVRLGERFADDEVGQIARAFDTYTGKMDDYVEKQMA
                     FAAMASHELRSPLTIVQTSADLIASRSADARILPHLEKIQRASSAMANMIHALLAVTR
                     DRPPERNLEDTRLRPLVDEIVESLEPEIRAKQITIDNRLDAASGVRSDPTLLTVVLTN
                     LIRNAVKHGDRSRIVIDLQHGRLSITDNGIGIDPESLQRIFYFGVRGQHSRGYGIGLY
                     ISRLVCEYQGWKLSLQPNPDGGIIAQIDFLG"
     gene            complement(4267..4947)
                     /locus_tag="VIW27_03190"
     CDS             complement(4267..4947)
                     /locus_tag="VIW27_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5738701.1"
                     /translation="MKILVIEDDADIASNIGQYFEERGHQLDFAYSGNHGLELALGKR
                     FDLLILDLMLPGKDGITLCREFRERTSYDTPVLMLTARDTLDDKISGFDAGADDYLVK
                     PFSLRELDARVHALTRRSRAPRPAQVISIGDLELNRATMQVTRAGQPVRLKPRAFRIL
                     EYLMDNADRVIPRQELIDHIWRDDPPDGDPLRVHIHNLRARIDKPFSRDLIHTVHGVG
                     YRIGTRAP"
     gene            5125..5718
                     /gene="msrA"
                     /locus_tag="VIW27_03195"
     CDS             5125..5718
                     /gene="msrA"
                     /locus_tag="VIW27_03195"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5738702.1"
                     /translation="MKRLLISILFAGTTIAAFGAPAATAVLAGGCFWCMEADFEKLDG
                     VTDVVSGFSGGSHPDPTYNGAHTGHYEAVEISYDPEVVDFQGLLDHFWVNVDPFDAHG
                     QFCDKGHSYLAAIFVGNDEERRLAEASKQRVIERFPGKTVVMPILERGEFFPIKGDEE
                     YHQDYYKKSPVRYQYYRWGCGRDNRLRQIWGDKVTVK"
     gene            5971..>6457
                     /locus_tag="VIW27_03200"
     CDS             5971..>6457
                     /locus_tag="VIW27_03200"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY5738703.1"
                     /translation="MGCQYFDPCEGGADTLTIAIPKYSFGRGCLREAGARAAGLGMSR
                     VGLFTDPFLRDSRYVATVLESLDAADIDVDTFDEITIEADDASVERGARFVAEGRFDG
                     VVSVGGGSVMDTAKASMLYGLHPTDEFLDYFAPPLGAGRTMPRSLLPHLACPTTSGTG
                     SE"
BASE COUNT         1156 a         1910 c         2140 g         1251 t
ORIGIN      
        1 aaccatcggc ccaaccacgg cgtttaacaa ataaaaatgt gcctcgaatc cggtctaccg
       61 gtaacaccag tacctgacgc cctgcgcatc gagaaaggtc tcggcgtcgc ctccctgcag
      121 catcgccagc gtcgacagga tgccggcctc ggtgcaggta gccgcggcga cggtcaccga
      181 gcgcggcgca tcggggctgg gccagccggt cttcgggttg agtatgtgac tgtagcgccg
      241 gccgcggtgt tcgatgtact tgaagacgtc gccgctggta gcgagcgcgc cctgtcgaag
      301 ccgaatcacg gcgctggcgt caccgggccg gttggggttc tcgatgccgg tcatccacgg
      361 ccggccgccg gcctgcgggc cgctggcgtg acagtcgccg ccgaaattga ccagcgcgct
      421 gcaatcggcc gcgtcgcgaa tcaggccgag cgccgaatcg acggcgtatt ccttgccgat
      481 gccgccgaaa tcgatttcca tgcccgccgg cagcgtcagg tacggctttt cccagctaac
      541 ctgatgccag ccgatgtgcg cgagcaggcg atcgatctcg cgctgtcgcg gaaggcgagc
      601 gccgtcgccg aatttccacg cgcgccgcag gataccggag gtgatgtcga accagccgtc
      661 gctgagctgg taacaattgt ccgcgaagtc gagcaagcgc gcggtttcct cgtcgacctc
      721 gaggcgttgg ccgccgcggt tgatgcgatg cacgatattg tcgtcgcggt agcggctgaa
      781 tttttgctcg atgcggaatg cttcgcggct ggcgatctcg atcagctcgt gggcctgcgc
      841 ccggtcgtcg cattcgagca gcacttcgca ggggctggcc atgcagcgaa agccgccgac
      901 ccagaggttg ccgcggcgcg tgatcgaggg agataccgtc atcgtttcga attctgccgg
      961 ccggcttatc gccgccagtg gccatagcgc gccgatgcgg cgcattgtaa cgccgccgcc
     1021 gtcgcgctag aagacgaaag agtaattgaa ttgtacgatc gtcgcctcga cgtcggggta
     1081 caggtcctgt tgcgcgagct ggccgaaggc ctcggacggc gaggattcgc cgctttgcac
     1141 gtattgttcg aggcgcaccg accattggtg gcggccgtcg atgtcgtggc cgaacttgac
     1201 gccgacggtg gtcgcgtcca tttcgccgag acgatagtcg gcgctggcct ctgccggcag
     1261 caggtcgccg tcgaccagga agtagcggta aaaatcggcc gcgctctgct ggtaatagcg
     1321 caggtgcggc tgcaggtaat aacgctcgcc gagccgaaag cggtaagtca ggtcgacggt
     1381 gttcgaatcc acgccccagt cgtcggtcat gtatcggtag gaggcggtga agatatcctt
     1441 gccgggcagc atcttccggt acttggcgaa aagcgccgag cgcagccggc tgtcggggcg
     1501 gttttcgaac aattggtcga tcggctcgcc gctgacgttg tcgaccacgc tgaccagctt
     1561 gtaggggtcg gtcaggtagc cgtcggccag gctcagcgac aggttgacct ggaacaggct
     1621 ggaacggtcg atgacctggg tgacgccgaa caacaggtcg acgatcgtgc gatcctcgga
     1681 acccgattgt ttccgctggt cgtccatcgg cgtcagcggc tcgggcgcgc cgccgaccgg
     1741 atcgatgacg tcgtactcga ggttgctgcc gaatgaccag gtggtgttct tctggttgct
     1801 gtcgagcgac cacaggccgt tcacggccag cgacgtgaag tcatactcgc gcgacaggtt
     1861 gaaaccgagg ttgcgccgcc ggtcgcggtc gagcggcttg tcccagttca tgctgtaggc
     1921 gacacgggta tccttgaacg acgggtcgag cggcgtttgg ttggcttcga cgacgtagct
     1981 gccgttgccg gacgggcggg tgaaagtctg cgggctggtg gatggcaccg caccgctcgc
     2041 cgacgagccg gtcaggctgt cgacgaccag tttccacgac aggatttcat cgtcgccgag
     2101 gtccttgcgt gccgcaatta ccggctcgat ggcctgaatg cggtcgtcgg cctcgctgta
     2161 gtagaggatc gacgtgtcga cctcccattc gccggccgcc tgcgactggc cgccgagcag
     2221 cgagcaggtc gctgccgaca gcaggccggc gatcttcttc ttgctcagtt gcatccgcaa
     2281 ccaccgccgg cgaagccctg tccgccgctg gaggcttcct tgctgaaata aatgtgttcg
     2341 tcgtaggcca ggtcgagcgc gtcggcattc atcagcatgt cgtcgcgcgc cagcaggtcg
     2401 cgttcccagg gcttgacgcc gagcgacgag cagccggccg ttagcagcag ggccgcgatc
     2461 agcagccagc gggttcgggc gatcataaca attcctcgat ttcgcgctcg tagcgctcga
     2521 cgtgcttggt gtagaagccg atgtgcttct tgactacctc gcccgtcggc gagatcaggt
     2581 acgaggtcgg catgccctcg atctggaatt gctcggcgag ccggccgctg ggatcatggt
     2641 agatgtcgaa atttgccggc accttgtcga gaaaacggtg catcgcagcg gtttcggcat
     2701 cgaggttgat ggtgatcacg gtgaagttct cggccgggta cttgacgagc agtgcgttca
     2761 tccacgggaa cgattcacgg cacggtttgc accaggatgc ccagaaatcc aggtagacga
     2821 ccttgccgcg atgggccttg aggtcgaggt cgccgatcgg gaaaatctcc gctcggagcg
     2881 gtgttgccgc gaccagtagc aacagcagga acagtttttt catgtggggt tcttcggcag
     2941 tcggtaacga gagtttgaca gcaaagttga tgctagcgtt cagcttgtat actcgatgtt
     3001 aaccgaggaa atcgatttgc gcgatgatgc cgccgtcggg attcggttgc agcgacagct
     3061 tccagccctg gtattcgcag accagccggg agatgtataa gccgataccg taaccacggc
     3121 tgtgctgtcc gcgaacgccg aaataaaaga tgcgctgtag cgactcgggg tcgatgccga
     3181 tgccgttgtc ggtgatcgac aggcgcccgt gctgcaagtc gatgacgata cggctgcggt
     3241 cgccatgctt gacggcgttg cgaatcaggt tggtcagtac cacggtcagc agggtcgggt
     3301 cggagcgcac gccgctggcg gcgtcgagcc ggttgtcgat ggtgatctgc ttggcccgaa
     3361 tctcgggctc cagcgactcg acgatttcgt cgaccagtgg ccgcagccgg gtatcctcca
     3421 ggtttcgctc gggcgggcga tcgcgggtta ccgcgagcaa tgcatggatc atgttggcca
     3481 tggcggaaga tgcgcgctgg attttctcga ggtgcggtaa aatgcgtgca tcggcactgc
     3541 ggctggcgat caggtcggcc gaggtttgca cgatggtcag cggcgagcgc agctcgtggc
     3601 tggccatcgc cgcgaacgcc atctgcttct cgacgtagtc gtccattttc ccggtatagg
     3661 tatcgaaggc ccgcgcgatc tggccgacct cgtcgtcggc gaaacgctcg ccgaggcgca
     3721 ccccgcgctg atcgggatcg atgtgcgaca gctcgttggc aagctgcgcg atcggccccg
     3781 atatgcggcg gctgaggatg cgtgcgataa agaatatcag caccagcagc accacccagg
     3841 ccaccaccac gatcacctgc atcaggtgtt cggagcgctc gatctcggtg acgtcgtact
     3901 ggatgtagat gcggtcgtcg ccgtagggtt cgaccagcac gtgataagcc ttgccgtcga
     3961 gcaccagatc gtggtgtacg ccgggatcga gattgacgag atgtcgtggt atcggccgcg
     4021 tcgcgcggcg gctgtggaga tagaccgaca ggctggccgc cgccggcagc ggtgactgag
     4081 gatcctgcgc cagcaactcg agagcgtttt ccagctcgaa gcgggtctgc gaatcgagca
     4141 ggtcgttttc gagcttctcg ttggagtaga agacgattgc cgacagcgcc gacaggaaaa
     4201 acaccgaggc cagcaggata ctggcggtga tcttgcgaaa caggggcgta tcgccgagca
     4261 gcgctttcat ggcgctcgcg tgccgatacg gtaaccgacg ccgtgcacgg tatggatcag
     4321 gtcgcgggag aacggcttgt cgatcctggc acgcaggttg tggatgtgga cgcgcagcgg
     4381 atcgccgtcg ggcggatcat cgcgccagat gtgatcgatc agttcctggc gtggaattac
     4441 ccggtcggcg ttgtccatca ggtactcgag gatacgaaat gcccgcggct tgagccgtac
     4501 cggctggccg gcgcgcgtca cctgcatggt cgcgcgattg agctcgagat cgccgatgct
     4561 gatcacctgg gccgggcgcg gcgcccggct gcgacgggtc agcgcatgca cgcgcgcgtc
     4621 gagttcgcgc agcgagaacg gcttgacgag gtaatcgtcg gcgccagcat cgaagccgct
     4681 gatcttgtcg tcgagtgtat cgcgcgcggt cagcatcaac accggcgtgt cgtaggaggt
     4741 gcgctcgcgg aactcgcggc acagggtgat tccgtccttg ccgggcagca tcaggtcgag
     4801 tatcagcaga tcgaagcgtt tgccgagcgc gagttccagc ccgtgattgc cgctgtaggc
     4861 gaaatccagc tgatgacccc gctcctcgaa atactgcccg atgttggaag cgatgtccgc
     4921 gtcgtcttcg ataacgagga tcttcatcat aaccaaggtt actcggcgcg ggtaaataaa
     4981 atgttaacaa acgggccacc gtgagcggag cgcacgctgc cgcctgccgg tagacttttc
     5041 cggcggcgtg ccaggaatgc agatattctt gctgaactca tcggctgcta atcggtccga
     5101 gggtttattt tacggaataa tccgatgaaa aggttgctga tttcgatact gtttgccggc
     5161 acgacgattg ccgcgttcgg cgcaccggcg gcgaccgcgg tcctcgccgg cggctgtttc
     5221 tggtgcatgg aagccgattt cgagaagctc gacggggtta ccgacgtggt ctccggcttt
     5281 agcggcggtt cgcaccccga cccgacctac aacggcgcgc acaccggtca ctacgaggcg
     5341 gtcgagatca gctacgatcc cgaggtcgtc gatttccagg gcctgctcga tcatttctgg
     5401 gtgaacgtcg atccgttcga cgctcacggg caattctgcg acaaggggca cagctacctg
     5461 gcggcgatat tcgtcggcaa cgacgaggag cggcgcctgg ccgaggcatc caaacagcgc
     5521 gtaatcgaga ggttccccgg caagacggtg gtgatgccga tcctcgagcg tggtgaattc
     5581 tttccgatca agggcgacga ggagtaccac caggattact acaagaagtc tccggtgcgt
     5641 taccagtatt accgctgggg ctgcggccgc gataaccgcc tgcggcaaat ctggggtgac
     5701 aaggttactg tcaagtaaac cgccgctcga ataaccgccc gaaaacggac ggtccggcct
     5761 cgagccggac tcgatcgccg tcgctgatcc gtcatgccgg cctcgcgccg gaatccattt
     5821 cagcggcccc actatcgagt tcgcgatgac ggcggtatat gaatacctgg tccggacaaa
     5881 tataaccggc tggccgggcc gaaggcggtt tgcgcgagta tcctgcgagg gtaatataag
     5941 aagtccagaa taattaaagg aattgtcgcc atgggctgtc aatatttcga tccctgcgag
     6001 ggcggtgccg ataccctgac catcgcgatt ccgaagtaca gcttcggccg cggctgtctg
     6061 cgcgaggcgg gtgcacgcgc cgccggtctc ggcatgtcgc gcgtcggcct gttcaccgac
     6121 ccgttcctgc gcgacagcag gtacgtcgcc acggtactcg aatcgctcga cgcggccgac
     6181 atcgatgtcg ataccttcga cgaaatcacc atcgaggccg acgatgccag cgtcgagcgc
     6241 ggcgcgcgct tcgtcgccga gggtcgcttc gacggcgtgg tctcggtcgg cggcggctcg
     6301 gtgatggata ccgccaaggc gtcgatgctg tacgggctgc acccgaccga cgagtttctc
     6361 gattacttcg cgccgccgct cggtgcgggc cgcacgatgc cacgctcgct gttgccgcat
     6421 ctcgcctgcc cgacgacctc gggtaccggt tccgagt
//
LOCUS       DATCZV010000145         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_169019, whole genome shotgun sequence.
ACCESSION   DATCZV010000145 DATCZV010000000
VERSION     DATCZV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169019"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VIW27_03205"
     CDS             complement(<1..655)
                     /locus_tag="VIW27_03205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5738704.1"
                     /translation="MTANPRKIGKYTIKSVIGEGATSIVYEGFDPDIVRRVAIKMLRA
                     NLVGGKVGEELLARFRREAISAARCVHPNVVSILEYGQQDDIPYIVMEYVDGVSVHSL
                     IKHRLKYGRGISLRRSLSIVSQLLAALHAAHKLNIVHRDIKASNVLILKNSGRIKLVD
                     FGMARITEHSDLTMIGSMIGTPRYMAPELRLGLEADSRADVFSAARLFIELLRMLAPD
                     "
     gene            complement(675..869)
                     /locus_tag="VIW27_03210"
     CDS             complement(675..869)
                     /locus_tag="VIW27_03210"
                     /inference="COORDINATES: protein motif:HMM:NF039406.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738705.1"
                     /translation="MAKTAALRIRIEPELHKRFIDTCKAQDIPASQVLREFMKQYVSM
                     HETTTQLDFFQQPGKTHAPD"
     gene            1205..2212
                     /locus_tag="VIW27_03215"
     CDS             1205..2212
                     /locus_tag="VIW27_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY5738706.1"
                     /translation="MDLLTQGLLGGALALSAAPRKESRLAAVIGFAAALVADADVLID
                     VADDSLLQIEYHRHFSHALVFIPLGALLAAAVLWPLLRRQLGFATIYLYALLGYATAG
                     VLDACTSYGTHLLWPFSETRVAWSIIAIIDPVFTLLLLSGLVLGWRRRRPGPARTALL
                     LASSYLLFGLWQHHEALEAAQALAERRGHTAERLLVKPTIANLVLWRSIYSSGDRFYV
                     DAIRIGPGPERIYPGVSAPAFDLERDLPGLPTNSLQALDIERFTALSEGFVAADPRRD
                     GVLIDIRYSMLPTSIAPMWGLDVKVASATQHARFELYRDRPDNYRELFIAMLLGRELP
                     E"
     gene            complement(2279..3598)
                     /locus_tag="VIW27_03220"
     CDS             complement(2279..3598)
                     /locus_tag="VIW27_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5738707.1"
                     /translation="MECADLEIGMYVCELDRPWLESPFMLQGFLIENEEDIEALKNAC
                     EFVYVDKIVESAKAAAKSPQPDPEMLADGRYRRPAAGPADEKSRIRLSREADMLRHPP
                     KKGQPIDKFFPGKKLVQYRDKTIWRNEKDKAKRAVDYLYGHIVQIMDQSLKSDHLDLQ
                     NVSKAVSPMVHSVIRNPDPCLWWATTRPATNYHHDSALRTSVFAAVLGRRLGLPVPDL
                     VSMAIGGMLFDIGKSRLENTILKGVSKFNDEEIALIRRHVEIGLKILDRSGIEDPQII
                     EYVAHHHERFDGSGYPQQLADDMIPAFGRIAGLVDCYSAMTSSRGYARIKSPAEAINQ
                     LYKLKDVHFHTDLIEEFIQTIGVYPVGALVEMTSDEVAVVVAQSRTRRLRPVILLLLD
                     ADKQPTSEPRYVELDKVAQLEDGSKYDIVRNLEPNAHGIDLPSIELI"
BASE COUNT          703 a         1189 c         1242 g          890 t
ORIGIN      
        1 aatccggcgc cagcatgcgc aacagttcga taaacagcct agccgccgag aacacgtcgg
       61 cgcgcgaatc ggcttccagc ccgagacgca gctcgggcgc catgtatctc ggtgtgccga
      121 tcatgctgcc gatcatcgtc aggtcggagt gctcggtgat acgcgccatg ccgaaatcga
      181 ccagcttgat gcggccgctg tttttcagga tcagcacgtt ggaagccttg atgtcgcggt
      241 gcacgatgtt gagcttatgg gccgcgtgca gtgccgcaag cagctgcgag acgatcgaca
      301 ggctgcgccg caggctgatg ccgcggccgt atttgagccg gtgcttgatc aggctatgca
      361 ccgaaacacc gtcgacgtac tccatgacga tgtacggaat gtcgtcctgc tggccgtact
      421 cgaggatgga gaccacgttg ggatgcacgc agcgcgccgc ggagattgcc tcgcgcctga
      481 accttgccag caattcttcg ccgaccttgc cgccgaccag gttggcacgc agcatcttga
      541 tcgccacgcg gcgcacgata tcgggatcga agccctcgta aacgatactc gttgcgccct
      601 cgccgataac cgacttgatc gtgtatttgc caatcttgcg agggttcgct gtcatcgtga
      661 aatccgcgtt tgattcaatc gggggcgtgg gtcttgccgg gctgttgaaa gaagtccagt
      721 tgcgtggtgg tttcatgcat ggagacgtat tgcttcatga attcccgcaa tacctgcgac
      781 gccggtatat cctgcgcctt gcaggtgtcg ataaaccgtt tgtgcaactc cggttcgata
      841 cgaatccgca gtgcggctgt tttagccatt gtgttgccat tggatacaca aatcttttag
      901 tcgccagtat acgcgttgag attcggacac tcaagtggta tttatgcaac gccgcgatgc
      961 cgcgggatcg gcaccgcagt ctttcgccat ggccaacgcc ggcgagcgca tgctgtcggt
     1021 ctgcggaatt tgatttatcg ggtttttggg gcaaactccg gtacgttcag gttttgcctt
     1081 acccctatct gccgcaggat atcgacactg actatctcga ccgccagaaa ttaccgctgc
     1141 cgcttgccga agctgcaatc ccgccattgc agatggacca gggtacattg acgcagagta
     1201 agctttggac ctgctgaccc agggcctgct cggtggcgct ttggcgctgt cggccgcgcc
     1261 gcgcaaggaa tcgcgcctgg cggcggttat cggcttcgcc gcggcgctgg ttgccgatgc
     1321 cgatgtgctg atcgacgtcg ccgacgatag cttgctgcag atcgagtacc accggcattt
     1381 ttcgcatgcg ctggtgttca ttccgctcgg ggctttgctc gccgcggccg tgctgtggcc
     1441 gttgctgcgc cgacagctcg gtttcgcgac gatctacctg tacgcgctgc tcggatacgc
     1501 gaccgccggc gtgctcgatg cctgtaccag ctacggcact cacctgctgt ggccgttcag
     1561 cgagacgcgc gttgcctgga gcatcatcgc gatcatcgac ccggttttta cgctgctatt
     1621 gctgagcggg ctggtactcg gctggcggcg aagacggccg ggaccggcgc gcaccgcgct
     1681 gctgctggcc tcgagctacc tgctgttcgg tctgtggcaa catcacgagg cactcgaggc
     1741 cgcgcaggcg ctggccgagc ggcgcgggca caccgcggaa cgcctgctcg tcaagccgac
     1801 gatcgccaac ctggtgctgt ggcgctcgat atactcgagt ggggatcgtt tctacgtcga
     1861 cgcgattcgc atcggacccg gtcccgagcg catttacccg ggagtctcgg cgccagcctt
     1921 cgacctcgaa cgggatcttc cgggattgcc gacgaacagc ctgcaggcgc tcgatatcga
     1981 gcgctttaca gcactgtccg agggcttcgt ggccgccgat ccgcgccgcg acggcgtgct
     2041 gatagacatt cgctactcga tgctgccgac cagcatcgcg ccgatgtggg gtctcgatgt
     2101 caaggtcgcg tcggccacgc agcatgcccg tttcgaatta tatcgcgacc gtcccgacaa
     2161 ttatcgcgag ctgttcatcg ccatgctgct ggggcgtgag ctgccggagt gaggcgatgg
     2221 cagcgggttg cgcggtgtcg gcgggtagtg cctgcttgcc cggggttggc ggtgaggctc
     2281 agatcagttc gatgctcggc aggtcgatgc cgtgcgcatt cggttccagg ttcctgacga
     2341 tatcgtactt gctgccgtct tccagctgcg cgaccttgtc gagttcgacg taacgcggtt
     2401 cggaggtcgg ctgcttgtcg gcgtcgagta acagcaggat caccggccgc agccggcgag
     2461 tgcgcgattg cgcgaccacg acggcgactt catcggaggt catttcgacc agcgcgccga
     2521 cgggatagac gccgatcgtc tggatgaact cctcgatcag gtcggtgtga aaatgaacgt
     2581 ctttgagctt gtagagctga ttgatcgcct ccgccggtga cttgatacgg gcgtagccgc
     2641 ggctgctggt catcgcgctg tagcaatcga ccagacctgc gatgcggccg aatgccggaa
     2701 tcatgtcgtc ggcgagttgc tgtgggtagc cgctgccgtc gaagcgttca tggtgatgcg
     2761 cgacgtattc gatgatttgc ggatcctcga tgccgctgcg atcgagtatc ttcagcccga
     2821 tttcgacatg gcggcggatc agtgcgattt cctcgtcgtt gaatttgctg acgcccttga
     2881 gaatggtgtt ctccagccgc gacttgccga tatcgaacag catcccgccg atcgccatcg
     2941 ataccaggtc cggtaccggt agtccgagcc gccgaccgag caccgcggcg aaaaccgagg
     3001 tgcgcagggc cgagtcgtga tggtagttgg tggccggtct cgtcgtcgcc caccacaggc
     3061 agggatccgg gttgcgaatc accgagtgca ccatcgggct caccgctttc gacacgttct
     3121 gcaggtcgag gtgatcggat ttcaggctct ggtccatgat ttgcacgatg tgaccataca
     3181 ggtaatcgac cgcgcgtttc gccttgtcct tctcgttgcg ccagatggtc ttgtcgcgat
     3241 actggacgag ttttttgccg ggaaaaaatt tatcgatcgg ctggcctttt ttcggcggat
     3301 gcctcagcat gtcggcctca cggctcagac gaatcctcga cttctcgtcg gcgggtcctg
     3361 ccgccggccg gcggtacctg ccgtcggcca gcatctcggg atccggttgc ggcgatttag
     3421 cggcggcctt tgcggactcg acgattttat cgacgtaaac gaattcgcag gcgtttttca
     3481 gggcctcgat atcttcctcg ttctcgataa gaaatccctg cagcatgaag ggcgattcca
     3541 gccacggccg atccagttcg caaacataca tgccgatttc gagatcggca cattccatgc
     3601 gaactttctc gatttttttc agccctgcct ctgcagcagt attagacatt cacgtacccc
     3661 gaatcgacgc cctggatcaa tagcctatct atagcttagc gcgctcggca atgcaaaggg
     3721 gaaattcaga gaatgcaacg agttacgcaa tatttctcac ttgtttacta actcccgggg
     3781 acgccgcgtt gcaggcctgt cgcgagccgt cggcaacgcc ggaacacgac tctcggtgcc
     3841 gctttcgggt cccgctcgag ttatcgccgc cgcggcagga atagggtgcg atcgtggaag
     3901 tccggcgagc gcatcgagca gcgggtcgat gcgggttgcc ggcaccgaca gtcgccggct
     3961 gacgtgaagg tagcgttagc gctggttacc cgttatccag caacgaattc gaagggggcg
     4021 aaaa
//
LOCUS       DATCZV010000146         4215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_63007, whole genome shotgun sequence.
ACCESSION   DATCZV010000146 DATCZV010000000
VERSION     DATCZV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63007"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VIW27_03225"
     CDS             <1..736
                     /locus_tag="VIW27_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738708.1"
                     /translation="SGYQLTDAGLRLLPLAEQIESSSHRLYQDIAGKDARLGGTVRVA
                     APEALGTQVIARHVAELRREHPDIRLELVAETRRLSLSKREADIAISLSRPDSGRLVA
                     WKLCDYRLRLYATRDYLESRAAIESPEDLAAHDFVSYIDDLIDMPELRFFDSTVKNAN
                     VVFRSTNVSAQFNAILDGIGLGLVHCFMAQREPRLQVVLPAQVSVERSYWLLVHEDLR
                     HVARVDAVCDFLTRSLSGNPRLMMGD"
     gene            complement(751..2622)
                     /locus_tag="VIW27_03230"
     CDS             complement(751..2622)
                     /locus_tag="VIW27_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5738709.1"
                     /translation="MPDSIKLLSTARFRPFFVTQLLGAFNDNLYKNGLTVFIAFQAAG
                     VSQQDSNTLVNVAAALFILPFFLFSAIAGQLADKYEKSVLIRRVKLLEIAIMGLGALA
                     FLLQSPAMLVAILFLMGAQSTLFGPVKYSLLPQALQPTELVGGNAMVETGTFVAILAG
                     LIVGVYLVGLGYPVIAVAVISVAVLGYLASRAIPRLPAAAPELEISFNIPRQTLNIMR
                     DARENRSVFYAVMGVSWFWFIGITYITQLPNYVRYELGGNEQVYVLLLAMFSIGIGAG
                     SFLCEKLSGRQVEIGLVPIGSLGLTVFGVDLYFSQGLSAGAELLTPITFLQQASSLRV
                     CADIVMLGISGGLFIVPLYALIQQRSDPHKCSRVIAANNVLNALFMVIASLYGLFALS
                     SGVSIPLLLLIMALMNAAVAIFIFTLVPEFIMRLLVWLLVHLVYRVDKQGLEHIPEHG
                     PALLVCNHVSFVDALVLAGCIRRPVRFVMYYRIFQLPLLRFIFRTAQAIPIAGAREDP
                     QMMERAFEAISDALRGGELVCIFPEGKLTENGELDSFRPGVSRILEHDPVAVVPLALR
                     GLWGSFFSRAHGKAMSRPFARGAFNRIELVAGGAVDAAKASPQRLRAAVLELRGDRL"
     gene            2932..>4215
                     /locus_tag="VIW27_03235"
     CDS             2932..>4215
                     /locus_tag="VIW27_03235"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY5738710.1"
                     /translation="MALLRSLFERDWFVGLIITLLFLIFAETGVFSGLDRQAYDLGTR
                     ISSAREPRDDIVIVAVDDESLQKLGAWPWSRDVLAQATLVLTQAKPSVIGFTIPFDTA
                     QYAGGLSSISYLRDVLVAERKLSRNVRRALDDTESTLRGDDKLAGAFRSGARIVLSMP
                     YIASERPASEVPATLPKYLKGFTLPDVSLDGDGGDGAGRAGRQVTRVGVLFPPIEKLV
                     HRAASVGVIGFNPRFNNEPLLVQYGEEFLPSFALMLAARSKGLSSEDIESRGGAGPTL
                     NGKTISADRSFRIYPRIYDDNDGKPAFESYSLIDVLNGSIDASRFQNKIIIVGLTSPR
                     LAGSLSTPSGQTISPTLATAHTVSSLLNNELFELPEWAGWAQRSLIVALGIYLMFALG
                     RFRTSTETLLSLFLLLVIFNTHFILMSSQALWVPMV"
BASE COUNT          851 a         1309 c         1298 g          757 t
ORIGIN      
        1 gagcggttac cagctgaccg atgccggcct gcgattgttg ccgctggccg agcagatcga
       61 gtccagctcg catcggctgt accaggatat cgccggcaag gatgcgcgtc tcggcggtac
      121 ggtgcgggtc gccgcccccg aggcgctcgg cacgcaggtg atcgcgcgcc atgtcgccga
      181 gctgcgccgc gagcatcccg atattcgtct cgagctggtc gccgaaaccc gccgcctgag
      241 cttgtccaag cgcgaggccg atatcgccat cagcctgtcg cgtcccgaca gcgggcgcct
      301 ggtggcgtgg aagctgtgcg attaccgcct gcggttgtat gcgacgcgcg actacctcga
      361 gtcgcgtgca gcgatcgaat cgcccgagga tctcgccgcg catgacttcg tctcctatat
      421 cgacgacctg atcgacatgc cggaactgcg tttcttcgac agtaccgtga aaaacgccaa
      481 cgtcgtgttt cgcagcacca atgtcagcgc ccagttcaac gcgatcctcg atggcatcgg
      541 tctcggtctg gtgcactgct tcatggccca gcgcgagccg cgcctgcagg tcgtgctgcc
      601 ggcgcaggtt tccgtcgagc gcagttactg gctgctggtg cacgaagatc tgcggcacgt
      661 cgcccgcgtc gacgcggtct gcgactttct gacccgttcg ctaagcggca atccacgcct
      721 gatgatgggc gactgaagcg acgcgcacgc ctaaagccga tcgccacgca actcgaggac
      781 tgccgcccgc agccgttgcg gggacgcttt agcggcgtcg accgcgccgc cggcgaccag
      841 ctcgatgcgg ttgaaggcgc cgcgggcgaa cggccgcgac atcgccttgc cgtgcgcgcg
      901 gctgaagaag ctgccccaca ggccgcgcag cgccagcggc accaccgcta ccgggtcgtg
      961 ctcgagaatg cggctgacac cgggccgaaa gctatcgagt tctccgtttt cggtcagctt
     1021 gccttcggga aaaatacaga ccagttcgcc gccacgcagc gcatcgctga tcgcctcgaa
     1081 agcgcgttcc atcatttgcg gatcctcgcg cgcgccggcg atcgggatcg cctgcgcggt
     1141 gcgaaaaatg aacctcagca gcggcagctg gaatattcgg tagtacatga cgaatcgaac
     1201 cggccgccga atgcagccgg cgagcaccag cgcgtcgacg aaactgacgt gattgcagac
     1261 cagcagtgcc gggccgtgct ccggtatatg ctccaatccc tgcttgtcga cgcggtagac
     1321 caggtgaacc agcagccaga ccagcagacg catgatgaac tcgggcacca gcgtgaaaat
     1381 aaaaatcgcc accgctgcat tcatcaacgc catgatcagc aacagcagcg gaatcgaaac
     1441 cccggacgac aacgcgaaca ggccatacag cgatgcaatg accatgaaca gggcattgag
     1501 cacgttgttg gcggcgataa cccgcgagca cttgtgcgga tcgctgcgct gctggatcag
     1561 cgcgtaaagg ggcacgatga acaggcctcc cgagatcccg agcatgacga tatcggcaca
     1621 gacgcggagg ctcgatgcct gctgcagaaa ggtgatcggg gtcagcagtt cggcgcctgc
     1681 cgacagaccc tggctgaagt aaaggtcgac gccgaacaca gtcagtccca gcgaaccaat
     1741 cggtaccagg ccgatttcga cctgccgccc ggagagtttt tcgcagagga aggaaccggc
     1801 accgatgcca atcgagaaca tcgccagcag cagcacataa acctgctcgt tgccgcccag
     1861 ttcgtagcgc acgtagttcg gcaactgggt gatataggta atgccgatga accagaacca
     1921 ggagacgccc atcaccgcgt agaacacgct gcggttttcg cgcgcgtcgc gcatgatatt
     1981 gagcgtctgc cgaggaatat tgaaactgat ctcgagttcg ggtgcggccg ccggcagccg
     2041 cggtatggcg cggctggcca ggtagccgag gacagcgacg ctgatcaccg cgacggcgat
     2101 taccgggtag ccgagcccga ccaggtagac gccgacgatc agcccggcaa gaatggcgac
     2161 aaaggtgccg gtttcgacca tcgcgttgcc gcccaccagc tcggtcggct gcagtgcctg
     2221 cggcagcagg ctgtacttga ccgggccgaa cagcgtcgac tgggccccca tcaggaacag
     2281 gatggccacc agcatggccg ggctctgcaa caggaaggcc agcgcaccga gccccatgat
     2341 ggcgatttcg agcagcttga cgcggcgtat cagtaccgac ttttcgtact tgtcggcgag
     2401 ttgtccggcg atcgccgaaa acaggaaaaa cggcaggatg aacagcgccg cggcgacatt
     2461 gaccagcgta ttgctatcct gctgggagac ccccgctgcc tgaaaggcga tgaaaacggt
     2521 caggccgttc ttgtacaggt tgtcgttgaa cgcacccagc agctgggtaa cgaaaaacgg
     2581 ccggaagcgc gcggtcgaca acagtttgat ggaatcgggc atcgttacct ctgctagctt
     2641 cgaacggacg gatccaatcc acaccgcgac attaacgccg tcgcaaccga tgcgacgggc
     2701 gatatcgccg cgagtcgctc ctctcagtca tgaaaaccgg tcccgatgac acgagtaacc
     2761 ccggacagaa gggaagatag ccgaaatggc ggcacgcatc acagatttgc ctcaaagtcc
     2821 ccgtcattcc cgcaggcgga tatatttttg ggcggtgcgg gcagataagg gctgcttcgt
     2881 gggctataaa gattgccact gtttgtcact atatgaaatg gcatagcttg tatggcgtta
     2941 ctgagatccc tgttcgaacg ggactggttt gtcggactga tcatcaccct cctgtttctg
     3001 atcttcgcgg aaacgggcgt gttttccggt ctcgaccggc aggcttacga tctcggcacg
     3061 cgtatttcct cggcccggga accgcgcgat gatatcgtca tcgtcgcggt cgacgacgag
     3121 tcactgcaaa agcttggcgc ctggccctgg tcgcgcgatg tgctggcgca agccacgctg
     3181 gtgttgacgc aggcaaagcc gagcgtcatc gggttcacca tccccttcga taccgcgcaa
     3241 tacgcggggg gactatcgtc gatcagctat ctgcgcgacg ttctggtagc cgagcgcaag
     3301 ctaagtcgca acgtcaggcg cgcgctcgat gacactgagt cgacgctacg cggcgacgac
     3361 aaactcgccg gggcattcag gtcaggcgcg cgcatcgtgc tgtcgatgcc ctatatcgcc
     3421 agcgaacgac ccgcgtccga ggtgccggca acgttgccga aatacctgaa aggatttacg
     3481 ctgcccgacg tcagcctcga cggggatggt ggcgacggag ccgggcgcgc cggacggcaa
     3541 gtcacccggg tcggcgtgct gtttccgccg atcgaaaaac tggtgcatcg cgccgccagt
     3601 gtcggcgtca ttggttttaa cccgcgcttc aacaacgagc cgctgctggt gcagtacggt
     3661 gaagaattct tgccgtcctt cgcgttgatg ctggcggcgc gcagcaaggg gctttcgagc
     3721 gaagatatcg agtctcgggg gggcgcgggc ccgacactga acggcaagac gatcagcgcc
     3781 gaccgcagct ttcgaatata cccgcgcatc tacgacgaca acgatggcaa gccggcattc
     3841 gagtcttatt cactgataga cgtgctcaac ggcagcatcg atgcaagccg ttttcagaac
     3901 aagatcatca ttgtcggcct gacctcgccg cgcctggcgg gttcgctgtc gacgccttcg
     3961 ggacagacga tctcgccgac gctcgcgacc gcgcacacgg tttcgagcct gctcaacaac
     4021 gaacttttcg aattgcctga atgggccggc tgggcgcagc gcagcctgat cgtcgcgctg
     4081 ggtatttatc tgatgttcgc gctcggccgg ttccgcacca gcaccgaaac ccttctcagc
     4141 ctgtttcttt tgctggtcat cttcaacacg cacttcatcc tcatgagctc gcaggccctg
     4201 tgggtgccga tggtg
//
LOCUS       DATCZV010000147         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_328008, whole genome shotgun sequence.
ACCESSION   DATCZV010000147 DATCZV010000000
VERSION     DATCZV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328008"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIW27_03240"
     CDS             <1..462
                     /locus_tag="VIW27_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5738711.1"
                     /translation="SGRQAFPHAVAIARRNEAELMVFHVVDNRDLQSLEGYMSDELWA
                     ELSNRNLDDARKILLGRKRDDLAIKNIEQLCAECIDDQPERPVLSYEIKVETGERLEK
                     ILDEAHAGGYDLLVIAKHGNRASVKDAVMGDTTRRVVRRCRIPVLVIPLDD"
     gene            complement(540..758)
                     /locus_tag="VIW27_03245"
     CDS             complement(540..758)
                     /locus_tag="VIW27_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY5738712.1"
                     /translation="MKTMIIDIDGMSCEHCVRHVTEALEALSGVAAVTVSLADKQASM
                     QTDDGFDEAAARDAVDEAGYEITGIRAA"
     gene            complement(797..>2712)
                     /locus_tag="VIW27_03250"
     CDS             complement(797..>2712)
                     /locus_tag="VIW27_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY5738713.1"
                     /translation="HMAGLPLPAWLAPESAPLAFALVQLALTLPVAWVGRHMYVNGLR
                     NLRHLAPNMDSLITVGTGSALLYSLLNTGRVAAGEAQMAGALYYETAAAILAFVMVGK
                     YLEAVSKGRTSDSIRKLMAIQPRTALVLRDGTEHEVAIDDIVPGDRLKVRPGERIPLD
                     GRVVEGATAVDESMLTGESMPVEKRVGDAVTGGSFNQNGGIVVEVERVGAETTLAQII
                     RLVEQAQGGKAPIARLADRVSGVFVPVVMGIALLSATAWLLAGQSLPFALSIFIAVLV
                     IACPCALGLATPTAIMVGTGKGAEFGVLIKGGEALERMQDIDVVVFDKTGTLTEGKPA
                     VTDIVAAQGFDEDELLRLAASAEKGSEHALGTAIVTEGERRGLERLPVTDFSAFPGRG
                     LGARVDGRALLIGNRALLRSNEIALPDEAPADALAGAGKTPVYIAIEGRYAGLIAIAD
                     RVRPESAAVVKALHDEGVRVVMLSGDNRLAAEAAAREVGISQVIAEVLPADKAATVKR
                     LQQQGKKVAMVGDGINDSPALAQADVGLAIGAGTDIAMETAQVVLMRGSVAGVLVAIR
                     LSRATLRNIRQNLFWAFAYNSAGIPIAAGLLWLFGGPLLNPMIAAAAMALSSVSVVTN
                     ALRLQRFKPGVEY"
BASE COUNT          469 a          905 c          892 g          446 t
ORIGIN      
        1 tcgggccgac aggcgtttcc gcacgcggtt gcgattgcgc gccgcaatga agccgaactc
       61 atggtcttcc atgtggtcga caatcgcgac ctgcaaagtc tcgaaggcta catgagcgac
      121 gagttgtggg ccgagttgag caaccgcaac ctggacgatg cgcgcaagat cctgctcggc
      181 cgcaagcggg acgacctggc gatcaagaat atcgagcagt tgtgcgcgga gtgtatcgac
      241 gaccagcccg agcggccggt attgagctac gaaatcaagg tcgagaccgg cgaacgcctg
      301 gaaaagattc tcgacgaagc gcatgccggc ggttacgact tgctggtgat cgccaagcac
      361 ggtaaccgcg cctcggtgaa ggacgcggtg atgggcgata ccacccggcg tgtggtccgc
      421 cgctgtcgca tacccgtgct ggtgataccg ctggacgact gacggcacgg gtcggaacga
      481 aattcaccgc gctccaacag ggccgaaaaa agcggcgggc gaacccgcat aaagcgattt
      541 caggctgcgc ggatgccggt gatttcgtaa ccggcctcgt cgaccgcgtc ccgcgcggcg
      601 gcttcatcga agccgtcatc ggtctgcata ctggcttgtt tgtctgcgag gctcaccgtc
      661 accgccgcga ccccgctcaa tgcctcgagc gcctcggtga cgtggcggac gcagtgttcg
      721 cagctcatgc cgtcgatatc gataatcatc gttttcatct tcgtttcctg tcgattcggt
      781 gcgattactg caccagtcag tattcgaccc cgggtttgaa gcgctgcaac ctgagcgcat
      841 tggtgaccac cgagaccgag ctcagcgcca tcgccgcggc ggcgatcatc gggttcagca
      901 gggggccacc gaacagccac agcaggccgg ccgcgatcgg gatacccgcg ctgttatagg
      961 cgaaggccca gaacaggttc tggcggatat tgcgcagcgt cgcgcgggac agccggatcg
     1021 ccaccagcac gccggcgacc gagccgcgca tcagcaccac ctgggcggtt tccatcgcga
     1081 tatcggtgcc ggcgccgatc gccagcccga cgtcggcctg tgcgagtgcc ggggaatcgt
     1141 tgatgccgtc gccgaccatc gccaccttct tgccttgctg ctgcagccgt ttcacggtcg
     1201 cggccttgtc cgccggcaag acttcggcaa tcacctgcga aataccgacc tcgcgtgccg
     1261 ccgcttcggc ggccaggcgg ttatcgccgc tcagcatcac caccctgacg ccctcgtcgt
     1321 gcagcgcctt gaccaccgcg gcgctctccg ggcgcacgcg atcggcgatc gcgatcagcc
     1381 cggcatagcg gccttcgatg gcgatataaa ccggggtctt gccggcgccg gcgagggcgt
     1441 ccgcgggcgc ctcgtcgggc agtgcgattt cattcgagcg cagcagcgcg cggttgccga
     1501 tcagcagcgc tcggccgtcg acgcgggcgc cgagcccgcg gccgggaaaa gcgctgaagt
     1561 cggtgaccgg caggcgctcg agcccgcggc gttcgccctc ggtgacgatg gccgtgccga
     1621 gcgcatgctc ggagcctttc tcggcgctcg ccgccagtcg cagcaattcg tcttcgtcga
     1681 atccctgcgc ggcgacgata tcggtcaccg cgggtttgcc ctcggtcagc gtgccggtct
     1741 tgtcgaacac cacgacgtcg atgtcctgca tacgttcgag cgcttcgccg cccttgatca
     1801 gcacgccgaa ctccgcgccc ttgccggtgc cgaccatgat cgcggtcggc gtggcgaggc
     1861 cgagcgcgca ggggcaggcg atgaccagca cggcgataaa gatcgacaac gcaaacggca
     1921 ggctttgacc ggcgagcagc caggcggtgg cggatagcag cgcgataccc atcactaccg
     1981 gtacgaagac accggacacc cgatcggcga gccgcgcgat cggcgccttg ccgccctgtg
     2041 cctgctcgac gagacgaatg atttgcgcga gcgtggtttc cgcaccgacc cgctcgacct
     2101 cgacgacgat gccgccgttc tggttgaaac tgccgccggt gacggcatcg ccgacgcgct
     2161 tttcgaccgg catgctttcg ccggtgagca tcgattcgtc gaccgcggtg gcgccctcga
     2221 cgacgcggcc gtcgagcgga atgcgttcgc cggggcggac cttgagccgg tctcccggca
     2281 cgatgtcgtc gatcgcgacc tcgtgctcgg tgccatcgcg caacaccagc gcggtgcgcg
     2341 gctggatcgc catcagcttg cggatcgagt cggaagtgcg ccccttggac acggcttcga
     2401 gatacttgcc gaccatcacg aaggcgagga tcgcggcggc ggtctcgtag tagagtgcgc
     2461 cggccatctg cgcctcgccc gcggccacgc gaccggtgtt gagtaacgag tacagcaacg
     2521 cggaaccggt gccgacggtg atcagcgaat ccatgttcgg cgccaggtga cgcaggttac
     2581 gcaagccgtt gacatacata tggcggccga cccaggcgac cggcagggtc aacgccagct
     2641 gcacgagcgc aaacgcgagc ggcgccgact ccggcgccag ccaggcgggc aacggcaggc
     2701 cggccatatg cc
//
LOCUS       DATCZV010000148        11125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_698555, whole genome shotgun sequence.
ACCESSION   DATCZV010000148 DATCZV010000000
VERSION     DATCZV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698555"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VIW27_03255"
     CDS             complement(<1..411)
                     /locus_tag="VIW27_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5738714.1"
                     /translation="MPESKEQKPERSEGDVNLSPRRSAWAERHLDADTAAMLDEDARY
                     FLHQSLSTPCLNALQASRGIYLEDVQGREIMDFHGNSVHQVGHGHPRVIEAIKAQLDE
                     LPFCPRRYTNRYAIDLARRLVELAPEGLDKVLFSP"
     gene            complement(525..1547)
                     /locus_tag="VIW27_03260"
     CDS             complement(525..1547)
                     /locus_tag="VIW27_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEY5738715.1"
                     /translation="MSEKVKIVRTDQELECPIIDNTLRDKGYRLVLLPDDVDSARLER
                     ETRDADLILMCYTPITADIIDKAENLKAIVKYGVGIDAIDIDAARRRKIPVVNIPEYA
                     EETVAEGAFSLMIALAKKLFPLRRVMQEEGWAWPERRWIGSDLAGKTVGLVGTGRIGR
                     SMARMAGAGFRARILGYDPNVSPSTMAAHGIEKYDDLNAMLMQSDFVSIHAVLNPDSH
                     GLIGANQLTAMKTSAFLINVSRGAIVDEAALLEALRSGQIAGAGLDVYSQEPLAKSGH
                     PLSELYAMENVILLPHLTFYTVDAMHRLEQETLQRCFEALEGRPVLIKSKDPRLLGQE
                     SGVAYV"
     gene            complement(1682..4141)
                     /locus_tag="VIW27_03265"
     CDS             complement(1682..4141)
                     /locus_tag="VIW27_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5738716.1"
                     /translation="MSSAIPERASAVVIGGGVIGSSVAFHLAKLGWSDVVLLERNQFS
                     CGTTWHAAGLIGTMRANESQARLCEYSMSVLAELEAETGQSTGFRQVGSLSLAHSEAR
                     FEELKRVAAMNNAFGVTQVDIITPQDVKSLYPFVETGDLLGASWVAQDGTASPVDITQ
                     AFVKGARGRGAACLEGVRVTGITRADGRVTGVVTDQGEIRADFVVNCGGLWGREIGRM
                     AGVSVPLHACEHYYAHTEKLDGLPANLPVMRDHDNCAYYREDAGSLLVGAFEPNAVPW
                     GHNGIPDDFAFEELEGHMDEQFMPVLEKAMARVPMLAECGWRSFFCGPESFTPDDQFH
                     MGEAPELKNFYVACGLNSVGIQTSGGLGRALADWMDKGHAPMDLWGNDIRRMFPFQST
                     QRYIESRVSETLGLLYENHYPYKQMRSARDLRHSPLHERLVEHNACFGEAAGWERANW
                     FAPAGVEPKYEYSFGKQNWFEYSAAEHRAAREKVVLFDQSSFSKYLVQGRDAVNVLQR
                     VSSADVDVEPGRMVYTHWLNEHGGIEADLTVTRLADDEFWVISGAAQTVKDLHWLKSH
                     IGDGEFCCVSDITNAWAMLGVMGPHSRALLGEVLDHEMSTAAFPFGSVQPVELGMARA
                     YAARVSYVGELGWELYVPVDQARHGFDYLWEKGRDHGLSLAGMHALNSCRLEKKFVHY
                     GHDVADHDTPLECGMGFVCAFDKAVPFIGRDAILAQQESRAFMRKRLVQFLLQDPDAV
                     LYHHEPILRDGVIVGHLTSGDYGHTLGGAVGIGYVEADEEITADYIAGGEFEIDVGGE
                     RIAARASLSAMYDPRAERMRG"
     gene            complement(4281..6704)
                     /locus_tag="VIW27_03270"
     CDS             complement(4281..6704)
                     /locus_tag="VIW27_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5738717.1"
                     /translation="MPIAKDIWLIYKLFLCLGLAYLVATAYSRWQEINSSSEVELAYL
                     NRVFSSSITLTFGQQEIMLDLLGRQIMTDESPDRWQRAERLLDGVLEKNDSLLAFGLA
                     NLDGEVIVGSSNLDLGKMPNLKRFDNSRSTFLKALEVDRMVLGRTYFLPALDDLVIPI
                     RKGIRDGENRLIGMMTAGIKARELLPELDAMSPGNNPDSPYQLRVFHDQDFYYAYVSG
                     IADQAQLQAIIDEPISQNYLQLVDRSLAEQRGLSLEQLRSQPGQVVFKATLQNGDSSL
                     YSLAYLPKYQLWTSAIVPRDFLLGQLYRSIAFYAFTFVVIFGLAFWLFRRIDASEQSS
                     RRKLIQQAGEDFLTGLKNRQYLRRAEKRWIHRQARPFSVFFIDLDNFKNINDSHGHSY
                     GDILLGQVADRLRSVFSDDDLVCRQGGDEFIVLCRRHDKAAISKQTEKVLRAIAASYY
                     VENYNFVIGASIGVARYPEDGNSFEELFSAADTAMYQAKNVKNSYAIFSSELREQLVE
                     TTFIQQALPQAISDRDFSLVYQPQVANAKSPVGVEALIRWRHQDRGLIPPDRFIPVSE
                     GNGMIIDIGHFVIDRALRDMAELAKTGRGRALKLSINVSIRQLQEAGFTRRLQNSLIE
                     SDFPPENLTLEITEGIFIDDLQYMIPILYEIRRLGVRLSLDDFGTGYSSLSLLKQLPI
                     DEMKIDKSFIDNIASNDEDRLMVLNIIDIAQNLNIDVVAEGIEQHEQSRMLIELGCHL
                     QQGYFYCHPVDLDQLVSYCEQTDWSFDEEEQEKNRKKGKLKDKDKDKDKDKRKHKHEN
                     KHKNRGNKQ"
     gene            complement(6842..7993)
                     /locus_tag="VIW27_03275"
     CDS             complement(6842..7993)
                     /locus_tag="VIW27_03275"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEY5738718.1"
                     /translation="MTAKPADTARSPSGDLYLLTPGPLTTSLTVKQAMLHDYGSRDAA
                     FIDLNARILERLVDIVGGRGTHVAVPLQGSGTFVVEAMIGNFVPAGGKLLILVNGAYG
                     ERIAKICDYYRRDYVVQQSPEDVPVDTAELDARLDADPAISHVAVVHCETTSGILNPV
                     AEVAEIVARHRRRLLIDSMSAFGALPLDAREIAFDAVVASSNKCLEGAPGMGFCVARE
                     TALVETEGNSPSLVLDLYDQWRAMQKNRQWRFTPPTHVLLAFDQALAEFEAEGGVAGR
                     GGRYRQNCELLVAGMRALGFETLLADELQAPIIITFRMPTNPEFDFAAFYDGLKELGY
                     VIYPGKLTVADSFRMGCIGRLGAEQINGALDAVGYLLARFGIERISSQP"
     gene            8085..8990
                     /locus_tag="VIW27_03280"
     CDS             8085..8990
                     /locus_tag="VIW27_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738719.1"
                     /translation="MPISHAHLRSFNAVATHGSFTRAAEMLNITQPTLSGQVKELEER
                     YGTRLFVRHGRRVELTEIGKSAFAITRLMFRHEEEVEQLLQSARALTSGQLRVGADSP
                     YIATPLLAQFQRLYPGIRISIRYGNSEELMHWIESRRCDVAFLPNIPQGDARLYSIPL
                     PPDRLVVFVHRDHDWAERRSVTIDELVTQRIVLREPGSRTRAIFEEAVSRAGHALHDV
                     MEIGGREGVREAVAAGFGIGVVAENELLADSRLRALPVSNAELAHAEYVVCLQESRGL
                     RVNEAFLEMVSSSQKDEPAGHRAGR"
     gene            9073..10323
                     /gene="phnA"
                     /locus_tag="VIW27_03285"
     CDS             9073..10323
                     /gene="phnA"
                     /locus_tag="VIW27_03285"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEY5738720.1"
                     /translation="MNSRRAINVNGRDYRWPDKPVVVVCIDGSEPDYIEQAIAAGVMP
                     WMQRIVEGAGSDLRADCVVPSFTNPNNISIVTGVPPVVHGICGNFYYDREGDREVMMN
                     EPELLRTPTIFKAFQDAGARVAIVTAKDKLRRLLGNELAFGEHGAICFSSEKADQVTL
                     AENGIDDVLGMVGMEVPSVYSAELSEFIFAAGVKLMKRVRPELMYLSTTDYIQHKFAP
                     GSDGANAFYAMMDKYWAQLDALGATIALTADHGMNAKHDAAGAPNIIYLQDELDALLA
                     PGAARVILPITDPYVVHHGALGSYATVYLPADADVNALQMQLADIDGIDAVYTNQKGC
                     ELFELAPDRMGDLILVSSRNVVIGTTAARHDLSGLDVPLRSHGGVSEQRVPLIVNRPL
                     DGLDAARPLRNFDAFDLALNHVVN"
     gene            10342..>11125
                     /locus_tag="VIW27_03290"
     CDS             10342..>11125
                     /locus_tag="VIW27_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5738721.1"
                     /translation="MTVKHEKMRIAGELVDGDCGTLEVFNPYNGEVVGTVPRASREQV
                     ARAFEIANAYKAKLTRYERQQILTRIAEIIISRKAEISDLITAECGLSKKDSLYECGR
                     AFDVYHLAGQLVINDDSEVFSCDLTPHGKQRKIFTQREPLRGVISAITPFNHPLNMIS
                     HKIAPAIATNNRLVGKPTEQTPLTALLLADICYEAGLPPQMLSIVTGLPADMGDEMIT
                     NPHIDLVTFTGSVPIGKYIAEKAGYRRTVLELGGNSELIVMDD"
BASE COUNT         2145 a         3486 c         3355 g         2139 t
ORIGIN      
        1 cggcgagaac agcaccttgt cgagcccctc gggcgccagt tcgaccagcc gcctggcgag
       61 gtcgatcgcg tagcggttgg tatagcggcg cgggcagaac ggaagctcgt cgagctgcgc
      121 cttgatcgcc tcgatgaccc gcggatggcc gtgaccgacc tggtgcacgc tgttgccgtg
      181 aaaatccatg atttcacggc cctgcacgtc ttcgaggtat atcccgcgac tcgcctgcaa
      241 cgcattcaga cagggcgtcg acaacgactg gtgcaggaag tagcgcgcat cctcgtcgag
      301 catcgccgcg gtatccgcat cgagatgtcg ctcggcccag gcgctgcgac gcggcgacag
      361 gttgacgtcg ccctcggaac gttccggttt ttgctctttg gattcaggca tctcgtgatc
      421 tcggttcgtt atcggtgttt acccctgcca ggaattcgcg gttcaagctc atacatcagg
      481 ccgcgtccag gggcgtcatc gggctgcaga cttcgacacc gaaatcaaac ataggccaca
      541 ccggattcct gcccgagtaa tctcgggtct ttggatttaa tcaggacggg gcgaccttcc
      601 agtgcctcga aacagcgctg cagggtttcc tgctccagtc gatgcatggc atccaccgtg
      661 taaaaagtca ggtgcggcaa cagtattaca ttctccatcg cgtagagttc gctgagcggg
      721 tggcccgatt tcgccaacgg ttcctggctg tatacatcga gacccgcacc cgcgatctgc
      781 ccgctacgca aggcttcgag cagcgccgcc tcgtcgacga tggcgccacg cgatacgtta
      841 atcaaaaaag cactggtctt catcgcggtc agttggttcg caccgatcag gccgtggctg
      901 tcgggattga gcaccgcgtg tatggacacg aaatcggatt gcatcagcat cgcgttcagg
      961 tcatcgtact tctcgatacc atgcgcggcc atggtagagg ggcttacatt cgggtcgtag
     1021 cccagaatgc gcgccctgaa accagcccct gccatgcgcg ccatactgcg accaatacgc
     1081 ccggtcccca ccagaccgac agtcttgccg gccagatcgc tgccaatcca gcgccgctcg
     1141 ggccaggccc agccttcctc ctgcatgacc ctgcgcaggg gaaagagttt tttggccagc
     1201 gcgatcatca ggctaaaagc gccctccgcc accgtttcct cggcatattc gggaatgttg
     1261 accaccggga tcttacgccg tctggcggca tcgatatcga tcgcatctat tccaaccccg
     1321 tacttgacga tcgctttaag attttcggcc ttgtcgatga tatcggcggt tatcggggtg
     1381 tagcacatca atatgaggtc ggcgtcccgg gtttcccgtt cgagacgcgc actatcgacg
     1441 tcgtcgggta acagcacgag ccggtagcct ttgtcgcgca gggtgttatc gataatcggg
     1501 cattcgagtt cctggtcggt gcggacgatc tttacctttt cgctcattcg ctttgtccac
     1561 gtgaagcatc agggccggac aggtgcccgg gataaaaaac cgccgccttc atattcgatt
     1621 catcctggtg cggggatggc gggccgccaa accgggtatg agggtcttta cgaataccga
     1681 cttagccgcg catgcgttct gccctggggt cgtacatcgc gctcaggctg gcgcgggccg
     1741 caatgcgctc gccgccgaca tcgatctcga actcgccgcc ggcaatataa tcggcggtga
     1801 tctcttcgtc ggcttcgacg taaccgatcc cgacggcgcc gccgagcgtg tgcccgtaat
     1861 cgcccgaagt caggtggccg acgatgacgc cgtcgcgcag gatcggctcg tggtggtaga
     1921 gcacggcgtc cggatcctgc agcaggaact gcaccaggcg cttgcgcatg aaggcgcgcg
     1981 actcctgttg cgcgagaatc gcatcgcggc cgatgaacgg cactgccttg tcgaacgcgc
     2041 agacgaagcc catgccgcat tccagcggcg tgtcgtggtc ggcgacatcg tggccgtaat
     2101 gcacgaattt cttttccagc cggcacgagt tcagcgcatg catgccggcc aggctcaaac
     2161 cgtgatcgcg gcctttttcc cacaggtaat cgaagccatg gcgggcctgg tcgacgggca
     2221 cgtagagttc ccagccgagt tcgccgacat aggaaacccg cgccgcgtat gcccgcgcca
     2281 tgcccagttc gaccggctgt accgagccga acgggaacgc cgcggtgctc atctcgtgat
     2341 cgagaacctc gccgagcagc gcacggctgt gcggacccat cacgccgagc atcgcccagg
     2401 cgttggtgat atcgctgacg cagcagaatt cgccgtcgcc gatatgcgac ttcagccagt
     2461 gcaggtcctt gaccgtctgt gccgcgccgc tgatcaccca gaactcgtca tcggcgagcc
     2521 gggtaacggt caggtcggcc tcgatgccgc catgctcgtt gagccagtgg gtgtaaacca
     2581 tacgtcccgg ctcgacgtcg acgtcggcgc tcgacacccg ctgcagcacg tttaccgcat
     2641 cacgcccctg caccaggtac ttggaaaacg aactctggtc gaagagcacg accttttcgc
     2701 gcgccgcgcg gtgctcggcg gcactgtact cgaaccagtt ctgcttgccg aaactgtatt
     2761 cgtatttcgg ctcgacgccc gcgggggcaa accagttggc tcgctcccag ccggccgcct
     2821 cgccgaaaca ggcattgtgc tcgaccagcc gttcgtgcag cggcgaatgc cgcaggtcac
     2881 gcgcgctgcg catctgcttg tagggataat ggttctcgta caacagtccg agggtttcgc
     2941 tgacgcggct ctcgatgtag cgctgggtcg actggaacgg gaacatgcgg cgaatgtcgt
     3001 tgccccacaa atccatcggc gcatggccct tgtccatcca gtcggcgagc gcgcgaccga
     3061 gtccgccgga agtctggata ccgaccgagt tgagaccaca ggcgacatag aaatttttca
     3121 gctccggcgc ctcgcccatg tggaactggt cgtcgggagt aaaactttcc ggaccgcaga
     3181 agaaactgcg ccagccgcat tcggccagca tcggcacacg cgccatcgct ttttcgagca
     3241 ccggcatgaa ctgctcgtcc atatggccct cgagttcctc gaaggcgaag tcgtcgggaa
     3301 taccgttgtg cccccagggc acggcgttgg gctcaaaggc gccgaccagc aaactgcccg
     3361 cgtcttcccg ataataggcg caattgtcgt gatcgcgcat caccggcaga ttcgccggca
     3421 ggccgtcgag tttctcggtg tgcgcgtagt agtgctcgca ggcgtgcagc ggcacgctga
     3481 ccccggccat acggccgatt tcgcgccccc acaggccgcc gcaattgacc acgaaatcgg
     3541 cgcgaatctc gccctggtcg gtgaccacgc cggtcacgcg gccgtcagcc cgggtgatgc
     3601 cggtaaccct gaccccttcg agacaggcgg cgccgcggcc acgcgcaccc ttgacgaacg
     3661 cctgggtgat gtcgaccggg ctcgcggtac cgtcctgcgc gacccagctg gcaccgagca
     3721 ggtcaccggt ctcgacgaac gggtacagcg acttgacgtc ctgcggggtg atgatatcga
     3781 cctgggtgac gccaaacgcg ttgttcatcg ccgcgacccg tttcaattcc tcgaaacgcg
     3841 cctcggagtg ggcgagcgac agcgagccga cctgcctgaa accggtcgac tgaccggtct
     3901 cggcctcgag ctcggccaac accgacatcg aatactcgca caatcgggcc tgcgattcgt
     3961 tggcacgcat ggtgccgatc agcccggccg catgccaggt cgtaccgcag ctgaactggt
     4021 tgcgttccag cagcacgaca tcgctccaac cgagcttggc gaggtgaaag gcgacactgg
     4081 agccgatcac gccgccgccg atgaccaccg ccgatgcccg ttcgggaatt gcactagaca
     4141 tggtttcgcc cacctcgatt tttattgtcg acaggcccgg tattctgcat taattaaatt
     4201 ggcgatagca aacggaatcg tccgtcgcgg gtaattagta gtgccggcaa tcgaccggcg
     4261 gctgccgccg cagatcgcga ttactgcttg ttgcccctgt tcttgtgctt gttctcgtgc
     4321 ttgtgcttgc gcttgtcttt gtctttgtct ttgtctttgt ctttgagctt gccctttttc
     4381 ctgttctttt cctgctcttc ctcgtcaaag ctccagtcgg tctgctcgca gtaactgacc
     4441 agctgatcga ggtcgaccgg gtggcaatag aaatacccct gctgcaggtg gcagccgagt
     4501 tcgatcagca tgcgtgactg ttcgtgctgc tcgataccct cggcgacgac atcgatgttc
     4561 aggttttgtg cgatatcgat gatgttcagc accatcagcc gatcctcgtc gttggaggcg
     4621 atattgtcga tgaagctctt gtcgattttc atttcgtcga tcggcaactg cttgagcagg
     4681 ctcagcgacg agtaaccggt gccgaaatca tccagcgaca ggcgaacccc gagacgcctg
     4741 atctcgtaga gtatcggaat catgtactgc aggtcgtcga taaagatccc ctcggtgatt
     4801 tccagggtca ggttctcggg gggaaagtcg ctctcgatca ggctattctg cagccggcgc
     4861 gtgaatccgg cctcctgcaa ctggcgaatc gaaacattga tcgacagttt caacgcccgg
     4921 cccctgcccg tctttgccag ctccgccatg tcccgcagcg cgcggtcgat gacgaagtgg
     4981 ccgatatcga tgatcatgcc attgccttcg gagaccggaa tgaagcgatc cggcggaatc
     5041 agccctcggt cctggtgccg ccagcgaatc agcgcttcga caccgaccgg cgacttggca
     5101 ttggcgacct gcggctggta gaccagcgaa aaatcgcggt cggagatagc ctgcggcagt
     5161 gcctgctgaa taaaagtcgt ctccaccagc tgctcgcgca gctcactgga gaatatcgcg
     5221 tagctgttct tgacgttctt ggcctggtac atggcggtat cggcggcact gaacagctct
     5281 tcgaaggaat tgccatcctc cgggtagcgc gccacgccga tgctggcacc gatgacgaaa
     5341 ttgtaatttt cgacgtaata ggacgccgcg atcgcgcgca gtaccttttc cgtctgctta
     5401 ctgatcgcgg ctttgtcgtg acggcggcag agcacgatga attcgtcgcc gccctggcgg
     5461 cataccaggt cgtcgtcgct gaaaaccgag cgcagccgat cggcgacctg cccgagcagg
     5521 atgtcgccgt aggaatgacc atggctgtcg ttgatgttct tgaaattgtc gagatcgatg
     5581 aagaacaccg agaacggccg tgcctgccga tggatccagc gtttctcggc gcgccgcaga
     5641 tactggcggt tcttaagacc cgtcagaaag tcctcgccgg cctgctggat cagctttcgc
     5701 cgactgctct gctcggacgc atcgatgcgg cggaacagcc agaacgcgag gccgaaaatg
     5761 accacgaacg taaacgcgta aaaagcgatc gaacggtata gctggccgag cagaaaatcc
     5821 cgcggcacaa tggcgctggt ccatagctgg tacttgggca ggtaggcgag cgaatacaga
     5881 ctggagtcac cgttttgcag cgtggccttg aacaccacct gcccgggctg gcttctcaac
     5941 tgttctagcg acaggccgcg ctgctcggcg agagaccggt cgaccagctg caggtagttt
     6001 tgcgaaatcg gttcgtcgat aattgcctgc agttgcgcct ggtcggcgat gccgctgaca
     6061 taggcataat aaaaatcctg gtcatggaac acgcgcaact ggtaaggcga gtcgggattg
     6121 ttgccgggac tcatcgcgtc gagttcgggc agcagttcgc gggccttgat acccgcggtc
     6181 atcatgccga tcaagcggtt ttcgccatcg cgaatgccct tgcggatcgg aatcaccagg
     6241 tcgtcgagtg cgggcagaaa ataagtgcgc cccaacacca tgcggtcgac ctccagggcc
     6301 ttgagaaatg tgctgcggct gttatcgaag cgcttcagat tcggcatctt gccgagatcg
     6361 aggttcgagc tgccgacgat gacctcgcca tccagattgg cgagaccgaa ggcgagcagc
     6421 gaatcgttct tttcgagtac cccgtcgagc agccgctcgg cacgttgcca gcggtccggg
     6481 gattcgtcgg tcataatctg ccggccgagc agatcgagca tgatttcctg ctgtccgaag
     6541 gtcagcgtta tcgacgaaga aaacactcga ttcaggtatg cgagttcgac ttccgagcta
     6601 ctatttattt cctgccagcg cgaataggct gtggcgacca ggtaagccag gcccaggcac
     6661 agaaacaatt tgtagatgag ccatatatcc ttggctatcg gcaccgcaat acccgatcga
     6721 tttttaatta cgccgagtat agttcaatcg agccatttca ctattcggcg caacggccgc
     6781 tgctcggcgc ccgccggccg cgggggaacc cagtccccgg gagcggcggc tgcgagcgtc
     6841 gctagggctg gctggagata cgctcgatac cgaaacgcgc cagcaaatac ccgacggcat
     6901 cgagcgctcc gttgatctgc tcggcgccga ggcgaccgat acagcccatg cggaaactgt
     6961 cggcgacggt cagcttgccc gggtagatca cgtagccgag ttccttgaga ccgtcgtaga
     7021 aggccgcgaa atcgaactcc gggttggtcg gcatgcgaaa ggtgatgatg atcggcgcct
     7081 gcaactcgtc ggccagcagg gtttcgaacc ccagcgcgcg catgccggcg accagcagct
     7141 cgcaattctg ccggtaacgg ccgccgcgac ccgcgacccc gccctcggcc tcgaactcgg
     7201 cgagcgcctg gtcgaatgcg agcagcacgt gggtcggtgg cgtgaagcgc cactggcgat
     7261 tcttttgcat cgcccgccac tggtcgtaga ggtcgagcac cagcgacggc gaattgccct
     7321 cggtctcgac cagcgcagtc tcgcgcgcaa cgcagaagcc catccccgga gcaccctcga
     7381 gacacttgtt gctggacgcg accaccgcat cgaaagcgat ttcacgagca tccagcggca
     7441 gtgcgccaaa cgcgctcatc gagtcgatca gcagacggcg ccggtgacgg gcgacgatct
     7501 ccgcgacctc ggcgaccgga ttcagaatgc ccgaggtggt ttcacagtgc accaccgcaa
     7561 catggctgat agcggggtca gcgtcgagcc gcgcgtcgag ttcggcggta tcgacgggca
     7621 cgtcctcggg cgactgctgc acgacgtagt cgcgccggta ataatcgcag attttggcga
     7681 tgcgttcgcc gtaggcgccg ttgacgagga tcaacagctt gccgccggcg ggcacgaagt
     7741 tgccgatcat cgcctcgacg acgaaggtac cgctgccctg cagcggcacc gcgacatgcg
     7801 tgccgcggcc gccgacgata tcgaccaggc gctcgaggat acgcgcgttg aggtcgataa
     7861 aggcggcatc gcgggaaccg taatcatgca gcatcgcctg ttttacggtc agcgacgtgg
     7921 tcagaggtcc cggggtcaac agatagaggt cgccgctcgg tgaccgggcg gtatcggcgg
     7981 gttttgcggt catgtttaag ccctgtgaaa cttgctcggt tgagatttat accgtcgata
     8041 gatactatcc atcaaatcga tatatttaat gcatcgaata gactgtgcca atatcacacg
     8101 cgcacctgcg ctcgttcaat gcggtcgcca cgcacggcag ctttacgcgt gccgcggaaa
     8161 tgctgaatat cacgcaaccg accctgtcgg gacaggtcaa ggaactcgag gagcgttacg
     8221 gcacccgcct gttcgtgcgc cacggtcggc gcgtcgagtt gaccgagatc ggcaagagcg
     8281 cattcgcgat cacgcgcctg atgttccgcc acgaagagga agtcgagcaa ttgctgcaat
     8341 cggcgcgtgc cctgacctcg ggccagttgc gggtcggtgc cgactcgcca tacatcgcga
     8401 cgccgttgct ggcccagttc cagcgccttt acccgggcat ccgaatttcg attcgctacg
     8461 gcaattccga ggaattgatg cactggatcg aatcgcggcg ctgcgacgtc gctttcctgc
     8521 ccaatattcc gcagggtgac gcccgcctgt actcgatccc gctgccgccc gaccggctgg
     8581 tggttttcgt ccaccgcgac cacgactggg ccgagcgacg ctcggtgact atcgacgaac
     8641 tggtcaccca gcgcatcgtg ctgcgcgaac cgggctcgcg cacgcgcgcg atcttcgagg
     8701 aagccgtgtc gcgggccggc cacgcactgc acgacgtgat ggagatcggc ggccgcgagg
     8761 gtgtgcgcga ggcggtcgcg gccggcttcg gtatcggcgt cgtcgccgaa aacgaactgc
     8821 tcgccgattc caggctgcgc gcgctgccgg tcagcaacgc cgagctggca cacgccgagt
     8881 acgtggtatg cctgcaggag tcgcgcggcc tgcgggtcaa cgaggcattt ctggagatgg
     8941 tgagcagcag tcagaaggac gagcccgcgg gtcatagagc gggtcgatag atggcatatg
     9001 taatatcgat ttaacccgca gtcgctaagc ggtatattgt gcgcctctat acttacgaat
     9061 agcggacaga tcatgaattc caggcgagcg atcaacgtca acggacgcga ttatcgatgg
     9121 cccgacaaac ccgtggtcgt ggtttgtatc gatggctcgg aacccgatta catcgaacag
     9181 gcgatcgccg ccggcgtgat gccgtggatg cagcgtatcg tcgaaggcgc cggcagcgac
     9241 ctgcgcgccg actgcgtggt gccgagtttc accaacccga acaatatctc catcgtcacc
     9301 ggcgtgccgc cggtggtgca cggcatctgt ggcaactttt actacgaccg cgagggcgac
     9361 cgcgaagtaa tgatgaacga gcccgaactg ctgcgcacgc cgaccatctt caaggccttc
     9421 caggatgccg gcgccagggt cgcgatcgtc accgccaagg ataagctgcg ccgcctgctc
     9481 ggcaacgaac tcgcctttgg cgagcacggt gcgatctgtt tttcgtccga gaaagcggac
     9541 caggtgacgc tggccgagaa cggcatcgac gatgtgctcg gcatggtcgg catggaagtg
     9601 ccgtcggtgt acagcgccga gctgtcggaa ttcattttcg ccgccggcgt caagctcatg
     9661 aagcgcgtgc gcccggagct gatgtacctg tcgaccaccg attacatcca gcacaagttc
     9721 gcgccgggca gtgacggcgc caacgcgttt tacgcgatga tggacaagta ctgggcccag
     9781 ctcgatgcgc tcggcgcgac tatcgcgctg accgcggatc acggcatgaa cgccaagcac
     9841 gatgccgctg gtgcacccaa tatcatctac ctgcaggacg agctcgacgc gctgctcgcg
     9901 cccggcgccg cgcgcgtgat cctgccgatc accgacccct acgtcgtgca ccacggcgcg
     9961 ctcggctcct atgccaccgt gtacctgccg gcggatgccg acgtcaacgc gctgcaaatg
    10021 cagctcgccg acatcgatgg catcgacgcg gtctatacca accagaaggg ttgcgaactc
    10081 ttcgaactgg cgcccgatcg catgggcgac ctgatactgg tgtcgagcag gaacgtcgtt
    10141 atcggcacca ctgccgcacg ccacgacctg tcgggtctgg atgtgccgct gcgctcgcac
    10201 ggcggcgtca gcgagcagcg cgtaccgctg atcgtcaacc gcccgctcga tggcctcgac
    10261 gcggcgcggc cgctgcgcaa cttcgacgcc ttcgacctgg cactcaacca cgtggtgaac
    10321 tgaactgata tggattctcc gatgaccgtc aaacacgaaa aaatgcgcat cgcgggcgaa
    10381 ctcgtcgacg gcgattgcgg tacgctcgag gtattcaacc cttataacgg cgaagtcgtc
    10441 ggcaccgtgc cgcgcgccag ccgcgagcag gtggcgcgcg ccttcgagat cgcgaatgcc
    10501 tacaaggcaa agctgacgcg ttacgagcgc cagcagatcc tgacccgcat cgccgaaatc
    10561 atcatttccc gcaaggcaga gatttcggat ttgatcaccg cggagtgcgg gctcagcaag
    10621 aaagattcgc tgtacgagtg cgggcgcgcc tttgacgtct accacctcgc cgggcaactg
    10681 gtgatcaacg acgacagcga agtgttttcc tgcgacctga cgccgcacgg caagcagcgc
    10741 aagatcttca cccagcgcga accgctgcgc ggcgtgatct ccgcgatcac gccgttcaac
    10801 catccgctga acatgatttc gcacaagatc gcgccggcca tcgccaccaa caaccggctg
    10861 gtcggcaagc ccaccgagca gacgccgctg accgcgctgc tgctcgccga catctgctac
    10921 gaagccgggc tgccgccgca aatgctgtcc atcgtcaccg gcctgcccgc ggacatgggc
    10981 gacgaaatga tcaccaaccc gcacatcgac ctggtgacct tcaccggcag cgtgccgatc
    11041 ggcaagtaca tcgccgaaaa ggccggttac cggcgcaccg tgctcgaact cggcggcaac
    11101 tccgaactga tcgtcatgga cgacg
//
LOCUS       DATCZV010000149         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_222336, whole genome shotgun sequence.
ACCESSION   DATCZV010000149 DATCZV010000000
VERSION     DATCZV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222336"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..431)
                     /locus_tag="VIW27_03295"
     CDS             complement(102..431)
                     /locus_tag="VIW27_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HEY5738722.1"
                     /translation="MTGKLIETLIMEGIAILMFVFAWLIGIKGKMELIAGYNERTAAR
                     VTDKAGLRRLIARTCLLVGIGSALMPFLTWIAVDHEAGLAHVIGGYGGFIVGVIGMVM
                     LQARDYT"
     gene            complement(453..1124)
                     /locus_tag="VIW27_03300"
     CDS             complement(453..1124)
                     /locus_tag="VIW27_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5738723.1"
                     /translation="MLIASEIGSVGRLVALGALLLASLVVTTIGHAAQPPSLEGKALV
                     EALRAGGYNIYFRHVATDWSQSDDVGSRNDWLDCDPARMRQLSDAGRDAAVAIGNAMR
                     RLKIPVFEVLASPYCRTQETARLMAVGPVEASTDVMNLRAAQYFGGRAAIVASARQLL
                     ASAPQPGSNRVIVAHGNVAREATPAYPSEGEGLVFKPGDDGEFYLVGRIEPEQWASLL
                     ELAAP"
     gene            complement(1131..1853)
                     /locus_tag="VIW27_03305"
     CDS             complement(1131..1853)
                     /locus_tag="VIW27_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5738724.1"
                     /translation="MKLSDFKLLTFDTYGTLIDWESGIYNALEPLLDKVPIKLGRDEV
                     LELFAEFEMQQQAATPSLVYSSLLAEVARSIAHKWQIKISDQEAFDFGRSVKHWPAFD
                     DSAEALNYLRRHYLMATVTNCDRISYMGSNARLEIEWDAIYTAQDIGCYKPNLRNFEY
                     MFERARRELGVLPHEILHVAQSLTHDMVPATSMGMTKVWINRRYNQEGLGATAPPQGD
                     YTIEWEFNSMADFVEAHRQEAA"
BASE COUNT          418 a          676 c          625 g          437 t
ORIGIN      
        1 gtggaactaa tgccgagcgc cgacgttggc gggcggggcg cgctggtcgc ggcgcgcatc
       61 gtcgccatgg tcatggggct ggccgcgcgc cagaaggatc actaggtata gtcgcgcgcc
      121 tgcagcatta ccataccgat aaccccaact ataaaaccgc catagccacc gatcacgtgt
      181 gccagcccag cctcatgatc cacggcaatc caggtcaaaa acggcattag cgcggaaccg
      241 ataccgacca acaggcaggt gcgcgcgatc aagcgcctga gtccggcctt gtcggtaacc
      301 ctggccgcgg tgcgctcgtt gtagcccgcg atcaactcca tcttgccctt gattccgatt
      361 agccaggcga acacgaacat cagtatcgcg atgccctcca tgatcagtgt ttcaatcaac
      421 tttccagtca tggaatatct cctcagtctg tgtcagggag cggccagctc cagcaagctc
      481 gcccactgtt ccggctcgat acggccaacc aggtaaaact cgccgtcgtc acccggtttg
      541 aaaaccaggc cctcgccctc gctcgggtag gccggcgtgg cttcgcgcgc aacgttgccg
      601 tgtgcgacga tgacccggtt gctgccaggt tgcggcgccg atgccaatag ttgccgcgcg
      661 ctggcgacga tcgcggcgcg gccgccaaaa tactgcgccg ctcgcagatt catgacatcc
      721 gtagaagcct cgaccggtcc gacggccatc aggcgcgcgg tttcctgggt gcggcaatag
      781 gggctcgcca gcacttcgaa gactggaatc ttcagtcgcc gcatcgcgtt gccgatcgca
      841 acggcggcgt cgcgaccggc atccgataat tgccgcatgc gcgcgggatc gcagtcgagc
      901 cagtcgttac gactgcccac atcgtcgctt tgcgaccagt cggtcgcgac gtgccggaag
      961 taaatgttgt acccaccggc gcgcagtgct tctaccaatg ccttgccctc gagactgggg
     1021 ggctgggctg catggccaat ggtagtgact accagactgg ccagcagcaa cgcgccgagg
     1081 gcgaccaacc tgccaaccga cccgatttcg gacgcaatca acatgagcgc ctaagccgct
     1141 tcctgccgat gcgcctcgac gaaatcggcc atgctgttga attcccactc gatcgtgtag
     1201 tcgccctggg gcggcgcggt cgcgccgagg ccttcctggt tgtaacggcg gttgatccag
     1261 accttggtca tacccatgct ggtggccggc accatgtcgt gcgtcagcga ctgcgcgacg
     1321 tgcagaattt catgcggcaa cacgccgagc tcacggcggg cgcgctcgaa catgtactcg
     1381 aagttgcgca ggttcggctt gtagcagccg atatcctgtg ccgtgtaaat cgcgtcccac
     1441 tcgatctcga gccgcgcgtt gctgcccatg tagctgatgc gatcgcagtt ggtcactgtc
     1501 gccatcaggt agtgcctacg caggtagttc aaagcctcgg cggaatcgtc gaacgccggc
     1561 cagtgcttga ccgaacgccc gaagtcgaag gcctcctggt cgctaatctt gatctgccat
     1621 ttatgcgcga tcgagcgcgc cacctcggcc agcagtgagc tgtaaaccag cgacggcgtc
     1681 gccgcctgct gctgcatttc gaactcggca aacagttcca gtacctcgtc ccgacccagt
     1741 ttgatcggca ccttgtccag taacggttcc agcgcgttgt aaataccgct ttcccagtcg
     1801 atcagcgtac cataggtgtc gaaggtcagt agtttgaaat cggatagttt catattgtcc
     1861 accggttcta ttcagtcagt gctcgggtta ttcgtctatt caccgggaaa ggattctaat
     1921 cgcatagggg gcatgccgtc tctgccaatt cgaatcgctc cctcgatctt aacgcgaatt
     1981 cgtcgcctcg agcgaccaga aaatccgggg ggaaacgcaa catactgcca ggcaggcagc
     2041 ggtggttgcc ggctagctga tctctaaatc agcagggcta gttgcggtag ctgttcggca
     2101 aatcgtagtg gtcgccgcgg aattcgccga atacttcttc cacttcggga ttgtgc
//
LOCUS       DATCZV010000150         4759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_328120, whole genome shotgun sequence.
ACCESSION   DATCZV010000150 DATCZV010000000
VERSION     DATCZV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328120"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1241)
                     /locus_tag="VIW27_03310"
     CDS             complement(48..1241)
                     /locus_tag="VIW27_03310"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY5738725.1"
                     /translation="MSHQLIDHMESALAEIRDQGLYKEERPLRSPQAAEVHAESWDQE
                     LINLCANNYLGLANHPQMIAAAHAGLDSHGFGMASVRFICGTQDIHKQLERRVADFVR
                     MDDAILFSSCFDANGGVFEVLLGEQDAVISDSLNHASIIDGIRLCKARRYRYANSDMA
                     ELEARLQEAADARFRLVVTDGVFSMDGTFANLPAICDLAEKHDALVMVDDSHAIGFTG
                     ERGAGTPELLGVEGRVHLTSGTFGKALGGASGGYITGPQPIIDMLRQRARPYLFSNSL
                     APCIVNATLTALDLLQQGGELRERLAANTAWFRERIVAAGFDVIDGTHPIVPIMTGDA
                     RIAQQLAAELMRHGVYVTAFSFPVVPEGKARVRTQMSAAHGREQLERALDAFVEVGRQ
                     IKLIS"
     gene            complement(1292..2485)
                     /locus_tag="VIW27_03315"
     CDS             complement(1292..2485)
                     /locus_tag="VIW27_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5738726.1"
                     /translation="MKDRPILLLAIAQTLIWACIFYSFPALLLHWEAALGWSRAELTG
                     AITLAIFMSAFCSPLYGRLIDAGKGAAMMTGASILGGCCMLLLTQVEFRWQFYLIWAV
                     IGVCMSGALYEPCFALITRARGAQARRSITFVTLVAGFAGTVSFPVAHGLAGEFGWRG
                     AALFFGLTAILVVSPIMWLGANAVEQGGAHRHLDTPPLAGARRAFLGSPVFWCLALGF
                     GFIAVTHGITLHHLLPILAERAIHPEVAVMAISFIGPMQVAGRLAMMAAERHVSSHGI
                     AIVCFLLIGASILMLIGAGSTPLMLVGFVILFGGAYGVVSIIRPVIARELLGERYFGA
                     KSGALALVYLAGSASAPYLGSLGWSWGGYRLVLPGLILLAAIGLGLYLSAQRLADADQ
                     GPGAV"
     gene            2699..3562
                     /locus_tag="VIW27_03320"
     CDS             2699..3562
                     /locus_tag="VIW27_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5738727.1"
                     /translation="MSNISVIGLGVMGSALAKTLLQNGYAVTVWNRSASKAAPLVEAG
                     ATQAGTAAEAINASPATIVCIATHDRTHELLEENAAALRGKTIIELSTGGAEEAEKLA
                     AMLQANGAEWLIGIINAYPQMIGEASTVLSIVGDPQVWARWEKVIRTLGGASSHFGNE
                     AGMAAVLFAALFTTRQGFMWGMLYGALVCRQAGIPLDVFSAQVPVSLGVLPGYHKYFA
                     DTAPSGNFDDPPASMNTYAAALDDALRTFESSGAPDELPRLFSELAHRGVDAGLGDQA
                     LTALIDLLVKK"
     gene            3609..4499
                     /locus_tag="VIW27_03325"
     CDS             3609..4499
                     /locus_tag="VIW27_03325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008846106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5738728.1"
                     /translation="MEKLTHIEIPQQAFDWYDEYAHGDIDRRTFMARLATLAGAGISL
                     SMLTSVLVPDYALAEQVSFNDPEIRASYATFASPNGHGEGRGYLVRPSQLELDAPAVL
                     VVHENRGLNPYVKDVARRLAKQGYVAFAPDALHPLGGYPGNDDEGRSMQRSMDRGKIE
                     QDFIAAARFLKAHELCNGKLGAVGFCFGGYITNLLAATLPEQIDAAVPFYGTPAKAEL
                     AGKVRGPVLLQFAEQDPRVNATWPEYEALLKKNGADYQAHVYPGVNHGFHNDSTARYD
                     REAAELAWSRTLEFFARHLG"
BASE COUNT          943 a         1580 c         1482 g          754 t
ORIGIN      
        1 ggccgggctc gtcgtgcgct tttaccaggg ctttcatgat gctctcctca ggaaatcagt
       61 ttgatctgcc gaccgacctc gacaaacgcg tcgagtgcac gctcgagctg ctcgcgaccg
      121 tgcgcggcac tcatctgtgt gcgcacgcgg gccttgccct cgggcaccac cggaaatgaa
      181 aacgcggtga cgtatacacc gtggcgcatc agttcagcgg ccagttgctg cgcgatgcgt
      241 gcgtcgccgg tcatgatcgg cacgatcgga tgagtaccgt cgatgacatc gaagccggcg
      301 gcaacgatac gctcgcggaa ccaggcggta ttggcggcca gccgttcgcg caactcgcca
      361 ccctgttgca acaggtcgag cgcggtcagg gtcgcgttga cgatgcacgg cgcgaggctg
      421 ttcgagaaca ggtaaggccg cgcacgctgg cgcagcatgt cgataatcgg ctgcggtccg
      481 gtgatatagc cgccgctggc gccgccgagc gccttgccga aggtgccgct ggtcaggtgc
      541 acgcggccct cgacgccgag cagttcgggc gtgccggcac cgcgctcgcc ggtaaagccg
      601 atcgcgtggc tgtcatcgac catcaccagc gcgtcgtgct tctcggccag atcgcagatc
      661 gccggcaggt tggcaaaagt gccgtccatc gaaaatacgc cgtcggtgac caccagccgg
      721 aaacgtgcat cggcggcctc ttgcagtctc gcttccagct ccgccatgtc gctattggca
      781 taacggtagc gccgcgcctt gcacagccga ataccgtcga tgatcgaggc atggttgagg
      841 ctgtcggaaa tcaccgcgtc ctgctcaccg agcaatacct cgaagacgcc gccgttggca
      901 tcgaaacaag acgaaaacag gatcgcgtca tccatgcgca cgaaatccgc gacccgccgc
      961 tcgagctgct tgtggatgtc ctgggtgccg cagatgaagc gcaccgaggc catgccgaag
     1021 ccgtgactgt cgagcccggc gtgcgcggcg gcgatcatct gcgggtgatt ggcgagaccg
     1081 agatagttgt tggcacacag attgatcagc tcctggtccc agctctcggc gtgcacctcg
     1141 gcggcctgcg gtgatcgcag cggacgctct tccttgtaga ggccctggtc gcgaatctcg
     1201 gcgagggcgg attccatgtg gtcgatcaat tgatgtgaca tacacaaact cccccggctg
     1261 gcgaattgcc actataacca aattttcgcc actatacagc gcccggacct tgatccgcgt
     1321 cagccaggcg ctgcgccgac agataaagac cgagcccgat agccgccagc aggatcagcc
     1381 cgggcagcac cagccggtag ccgccccacg accagcccag cgagcccagg taaggcgccg
     1441 acgccgaacc ggcgaggtac accagcgcca gcgcgcccga cttggcgccg aagtagcgtt
     1501 cgccaagcag ttcgcgcgcg atcaccgggc gaatgatgct gaccacgccg taggcgccac
     1561 cgaacaggat tacgaaaccg accagcatca gcggcgtgct gccggcaccg atcagcatca
     1621 ggatcgacgc gccgatgagc aggaaacaga cgatcgcgat accgtgactc gacacgtggc
     1681 gctcggcggc catcatcgcc agcctgccgg cgacctgcat cggtccgatg aaggatatcg
     1741 ccatcaccgc gacctcggga tgaatcgcac gctcggcgag aatcggcagc aggtgatgca
     1801 aggtgatgcc gtgggtcacg gcgatgaagc caaagccgag cgcgagacac cagaataccg
     1861 gcgaacccag gaaggcgcgc ctcgcccccg ccagcggtgg cgtatcgaga tggcggtgcg
     1921 cgccgccctg ctcgaccgcg ttggcgccga gccacatgat cggcgagacc acgaggatcg
     1981 cggtgaggcc gaagaatagc gccgcgccgc gccagccgaa ttcacccgcg agcccgtgcg
     2041 cgaccgggaa actcaccgtg ccggcgaaac ccgcgaccag cgttacgaag gtgatcgagc
     2101 ggcgcgcctg cgcaccgcgc gcgcgggtga tcaatgcgaa gcacggctcg tacagcgcac
     2161 ccgacatgca aacgccgatc accgcccaga tcaggtagaa ctgccagcgg aactcgacct
     2221 gcgtcaacag cagcatgcag cagccgccga ggatcgacgc gccggtcatc atcgcggcgc
     2281 ccttgccggc atcgatcagg cggccgtaca gcggcgagca gaacgccgac atgaaaatcg
     2341 ccagcgtgat cgcgccggtc agctcggcgc ggctccagcc gagcgctgct tcccagtgca
     2401 gcagcagcgc cgggaaactg taaaagatac aggcccagat cagcgtctgc gcgatggcca
     2461 gcaacaggat gggacggtct ttcataggca agcctgtatt gtgttgctat gatgccatcc
     2521 aatgatccgc agggatacac ggtcgtcgcg acgctgcaag ccggtatctt gccgggtggg
     2581 ccacgcggag caagtcagcc accatggatc agggttcacg ttctgcacag atcatgatat
     2641 gcgccggagg cggcgcacgc ggtgccatgc gccgttgcat caataacggg ggagaacaat
     2701 gtcgaacatc tcggttatcg ggctcggtgt gatgggcagt gcgctggcaa agacattatt
     2761 acagaacggt tacgcggtga ccgtctggaa ccgcagcgcg agcaaggcag caccgctggt
     2821 cgaggccggt gcgacgcagg cgggcactgc cgccgaggcg atcaacgcga gcccggcaac
     2881 gatcgtctgt atcgcgaccc atgacagaac ccacgaattg ctcgaggaaa acgcggccgc
     2941 gctgcgcggc aagacgatca tcgaactcag caccggcggt gccgaggaag ccgaaaagct
     3001 cgccgcaatg ctgcaggcga acggcgccga atggttgatc ggtatcatca atgcctaccc
     3061 gcagatgatc ggcgaagcga gcaccgtgct cagcatcgtc ggcgacccgc aggtctgggc
     3121 gaggtgggaa aaagtaatca ggacgctcgg tggcgcttcc agtcacttcg gcaatgaggc
     3181 cggcatggcc gcggtgctgt tcgccgcgct gttcacgacg cgccagggtt tcatgtgggg
     3241 catgctctac ggcgcgctgg tttgccgcca ggccggcata ccgctcgacg tgttctccgc
     3301 acaggtcccg gtttcgctcg gcgtgctgcc gggctaccac aagtatttcg ccgataccgc
     3361 gccgagcggc aacttcgacg atccgccggc cagcatgaac acctacgccg cggcgctcga
     3421 tgacgcgctc cgcaccttcg agtcgagcgg cgcgccggac gaattgccgc ggctgttcag
     3481 cgaactcgcg caccgcggag tcgacgccgg cctcggcgac caggcgttga ccgcgctgat
     3541 cgatttactg gtcaaaaaat aagcattttc gaaatcgttc gaggcagtct attcccggag
     3601 aaataaagat ggagaagctc actcatatcg agattccgca gcaggccttc gactggtacg
     3661 acgagtatgc ccacggcgac atcgaccgcc gcactttcat ggcacggctg gcgacgctgg
     3721 ccggcgccgg catcagtctc agcatgctca ccagcgtgct ggttcccgat tacgcgctcg
     3781 ccgagcaagt ctctttcaac gatcccgaga ttcgcgccag ttacgcgacc tttgcctcgc
     3841 cgaacggcca cggcgagggc cgcggctacc tggtcagacc gagccagctc gaactcgacg
     3901 cacccgcggt gctggtggtg cacgagaatc ggggactcaa tccgtacgtc aaggacgtcg
     3961 cgcgtcgtct cgccaaacag ggctacgtcg ccttcgcacc cgatgcattg cacccgctcg
     4021 gcggttatcc cggcaatgac gacgagggcc gcagcatgca gcgctcgatg gaccgcggca
     4081 agatcgaaca ggatttcatc gccgcggcac gcttcctgaa ggcgcacgaa ctctgcaacg
     4141 gcaaactcgg cgcggtcggc ttctgcttcg gcggttacat caccaacctg ctggccgcga
     4201 cgctgcccga gcagatcgac gccgcggtgc cgttctacgg cacgccggcg aaggcggagc
     4261 tggccggcaa ggtccgcggt ccggtactgc tgcaatttgc cgagcaggat ccgcgcgtca
     4321 acgcgacctg gccggaatac gaggcattgc tgaagaagaa cggcgccgac taccaggccc
     4381 atgtctaccc gggcgtcaac cacggctttc acaacgattc cactgcgcgc tacgaccgcg
     4441 aggcggcgga actggcctgg tcgcgcacgc tggaattttt cgcccgccac ctcggctgaa
     4501 gcggcctcga taaaccgggg tcaggctcga tctaccgagc gaagccccgc caattgcccc
     4561 tgaccccatt gttgtacagc ggcaaataag cattgccgtc cgcgcgggtc atgcccgcac
     4621 ccctactcgc gtcatccccg catacctgcc cgagtcatcc cccgcatacc tgcccgagtc
     4681 accccgcata cctatccgag tcatcccccg catacctgcc caagtcatcc ccgcatacct
     4741 gcccgagtca tccccgcat
//
LOCUS       DATCZV010000151         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_645652, whole genome shotgun sequence.
ACCESSION   DATCZV010000151 DATCZV010000000
VERSION     DATCZV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645652"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VIW27_03330"
     CDS             <1..145
                     /locus_tag="VIW27_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009680941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5738729.1"
                     /translation="HQFRLRWQRNTLAMWDNRCTQHYALNDYHGHRRVMHRIIVAGKP
                     PHP"
     gene            complement(273..1433)
                     /locus_tag="VIW27_03335"
     CDS             complement(273..1433)
                     /locus_tag="VIW27_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5738730.1"
                     /translation="MTAPRSADTRTLVAACAAITVFGFAFGMTYPLLSLILESRGVAS
                     DMIGINSAMMPLGILLISPAIPYATRRFGGRNVAITAAVVTAFAILAYKAFDTLAAWF
                     VIRLVQGMTISVLFVLSEAWITESAGDANRGKVVAIYASVLSASFGAGPLLISFIGID
                     GWTPFVLGAIVIVFGIIPFTLIRDNPQADPDESTPSGFFEFAPKAPMLLAAVGIFSIF
                     DAATLSLFPVYGIYNGLDLATSANLLTALILGNMLLQLPIGWLADRVEHRWVLAGCAL
                     TASVCLLMVPALIHSPLKWPLLVVAGTAGYGVYTVSLASLGNRFSGIELVNGASAFAL
                     VWGFGALFGSISGGWVMLGFGPHGLPVALALTYLLLAIGVSRRQLKLSHPPP"
     gene            1550..>2554
                     /gene="ltaE"
                     /locus_tag="VIW27_03340"
     CDS             1550..>2554
                     /gene="ltaE"
                     /locus_tag="VIW27_03340"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEY5738731.1"
                     /translation="MAEQAIVDLRSDTVTRPGAGMYQAIREAPVGDDVYGEDETVNRL
                     EALAAARLGKQAGLFVSSATQANLVAMLAHNARGEEILLGDEYHIFVSEAGGASALGG
                     VVMQPLPTDDNGGLALAQVLAAIKEDDPHKPITRTLALENTVSGQVQDPAQHRALAGA
                     VRPLGLRVHLDGARLMNAAVALGVNAAEIAEPFDSVMLCLSKGLGAPVGAVLCGAADF
                     IARARRLRKQLGGGMRQAGVLAACGLYALDHNVERLAADHVRAERLAEGLGEIAGLPV
                     RCATNMVFVEPRGEDCEPLAAHLRERGVEIGNQHPRLRLVTHLDVDDDGIERAIDGFR
                     A"
BASE COUNT          515 a          803 c          858 g          378 t
ORIGIN      
        1 gcaccagttc cgcctgcgct ggcagcgcaa tacgctggcg atgtgggata accgctgcac
       61 tcagcactac gcactcaacg attatcacgg acaccgccgc gtgatgcacc gcatcatcgt
      121 cgcaggcaag cctccccacc cctaatttgg gtcagatcca aattaaatcg acctccttaa
      181 ttcgggtcag atccaaatta agagcgctcg cgctaactcg ggtcagatct aaattaaagc
      241 gcgcgttcta atttagatct ggcccaaatt agtcaagggg gagggtggga cagtttgagc
      301 tggcggcggc tgacgccgat ggcgagcagc aggtaagtca gcgcaagtgc gaccggcaaa
      361 ccgtgggggc cgaagccgag catcacccag ccgcccgaga tcgagccgaa cagcgcgccg
      421 aatccccaca ccagcgcgaa cgccgatgcg ccgttgacca gttcgatgcc gctgaaacga
      481 ttgccgagcg aggccagcga cacggtataa accccgtagc cggcggtgcc ggcgacgacc
      541 agcagcggcc acttgagcgg cgagtggatc aaggccggca ccatcagcag gcaaaccgat
      601 gctgtcagcg cacagccggc gagcacccag cgatgctcga cccggtcggc gagccagccg
      661 atcggcaatt gcagcagcat gttgccgagg atcaacgcgg tcagcaggtt ggccgaagtc
      721 gccaggtcga gcccgttgta aatgccgtaa accgggaaca gcgacagggt cgcggcatcg
      781 aaaatcgaga agatgccgac cgcggccagc agcatcggtg ccttcggcgc gaactcgaag
      841 aagccggaag gcgtggattc atccgggtcg gcctgcggat tgtcgcggat cagcgtaaac
      901 ggaatgatgc cgaagacgat cacgatcgcg ccgagcacga atggagtcca gccgtcgata
      961 ccgatgaagc tgatcagcag cggcccggcg ccgaaactgg cggacaacac gctggcgtag
     1021 atagcaacca ccttgccgcg gttggcgtcg ccggcggact cggtaatcca ggcctcgctc
     1081 aacacgaaca gcaccgagat cgtcatcccc tgcacgaggc ggatcacgaa ccaggccgcg
     1141 agagtatcga atgccttgta ggcgaggatc gcgaacgcgg tgaccacagc cgcggtgatc
     1201 gcaacgttgc gcccgccgaa gcgccgggtc gcgtaaggga tcgccggcga gatcagcaga
     1261 atgccgagcg gcatcatcgc cgagttgatg ccgatcatgt cgctggcgac tccgcgcgac
     1321 tcgagaatca gcgacagcag cgggtaagtc atgccgaacg cgaagccgaa cacggtgatc
     1381 gcggcgcagg cggcgaccag ggtgcgggtg tcggcggagc ggggtgctgt catgcgcgaa
     1441 ttctacccga gccgccgaag tccggcaggc gatattttac ccctcgcagg cattcccatc
     1501 atcgccaaag cggcctacta ttaacggcga gtacaatgcg gaagttgtga tggccgagca
     1561 agcgatagtc gacctgcgca gcgatacggt aacgcgcccg ggcgcgggaa tgtaccaggc
     1621 gattcgggaa gccccggtgg gggacgatgt ttacggcgag gacgagacag taaatcgtct
     1681 cgaggcgctc gccgcggcgc gtctcggcaa gcaggcgggc ctgtttgtga gcagtgcaac
     1741 gcaggccaac ctggtggcga tgctggcgca caacgcgcgc ggcgaggaaa ttctgctcgg
     1801 tgacgaatac cacatcttcg tctccgaggc gggcggcgcg tcggcgctcg gcggtgtcgt
     1861 gatgcagccg ctgccgaccg acgacaacgg cggcctggcg ctcgcacagg tgctcgctgc
     1921 gatcaaggaa gacgacccgc acaaaccgat cacgcgcacg ctggcgctcg aaaacacggt
     1981 ttcggggcag gtgcaggatc cggcgcaaca tcgtgcgctt gccggcgcgg ttcgccctct
     2041 gggcctgcgc gtacatctcg atggcgcgcg cctgatgaac gcggcggtgg cgctcggcgt
     2101 gaacgcggcc gagatcgccg agccgttcga cagcgtcatg ctgtgcctgt caaagggcct
     2161 cggcgcgccg gtcggcgcgg tgctctgcgg agcggcggat ttcatcgccc gcgcgcggcg
     2221 gttgcgcaag caactcggtg gcggcatgcg ccaggccggg gtgctcgccg cctgcggcct
     2281 ttacgcactc gaccacaatg tcgagcggct ggccgccgat catgtgcgcg ccgaacggct
     2341 ggccgagggg ctcggcgaaa tcgccggcct gccggtgcgc tgcgcgacca acatggtatt
     2401 cgtcgaaccc cgcggcgaag actgcgaacc gctcgccgcc cacctgcgcg agcgcggggt
     2461 cgagatcggc aaccagcatc cgcgattgcg cctggtgact catctcgatg tcgacgacga
     2521 cggcatcgag cgcgcgatcg acgggtttcg cgct
//
LOCUS       DATCZV010000152         3892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_540655, whole genome shotgun sequence.
ACCESSION   DATCZV010000152 DATCZV010000000
VERSION     DATCZV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540655"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VIW27_03345"
     CDS             <1..723
                     /locus_tag="VIW27_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY5738732.1"
                     /translation="ETPANPTLKLTDIAAVSAVAQKHGIPHVVDNTFLTPFYQRPLEL
                     GADITLHSTTKFMEGHNISVGGSITAANPAHLEQIMFVRNCLGSNMTPMVAFYTLQGI
                     KTMSTRLEVQSANALKVAQFLETHANVERVAYPGLESFPQKELADRQASGHGSMLWFE
                     VKGGVAAGKKLMDTLELWSLAENLGSVESLVTHSVTMTHADMPREERLAAGITDGLVR
                     LSAGLESADDLIADLRQALDAL"
     gene            726..1835
                     /locus_tag="VIW27_03350"
     CDS             726..1835
                     /locus_tag="VIW27_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY5738733.1"
                     /translation="MIVTSIESFATEYVALVRVRTDDGDEGWGQISPYMADLSAQVLH
                     RQVAPHVLGMDAADIDAVTAAVLDREHKFQGTYLYRALCGVDTALWDIRGKRAGKSVQ
                     QLLGGVHAEFPVYASSMQREITPEAEAERMLALREQYGYRAFKFRIGRECGHDADQWP
                     GRTEAIVKLMRDTLGDDATLLVDANSCYSPQKAIEIGHLLTDYGISHFEEPCPYWELD
                     WTRQVTQALDIDVTGGEQDNNMIVWKQMIDSRAVDVIQPDICYMGGITRTLQVADYAH
                     RAGLPCTLHSANLSLVTLFSMHFMAAIDNPGKYVEFSIEGLDYYPWQDRIFTPGYQIR
                     DGNAQISAAPGWGIEINPEWLTLADYRVSYNGSRF"
     gene            1856..3223
                     /locus_tag="VIW27_03355"
     CDS             1856..3223
                     /locus_tag="VIW27_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5738734.1"
                     /translation="MTIVNSILELIGNTPILKLSKFDTGPCELFLKLESENPGGSIKD
                     RIAVCMIEAAEKEGKIEPGGTIIEATAGNTGLGLGLVAAQKGYKLILVIPDKMSPDKI
                     RHIRAMGVDVRLTRSDVQKGHPEYYQDYAKRLATEIPNSYFIDQFSNPNNPKTHETMT
                     GPEIYRQMDGRLDAVVVGVGSSGTVTGLGKYFHRELPDLEMVVADPVGSILADTINTG
                     NTPQASSWLVEGIGEDFLPPICDISLVKKAYAIPDREAFATQRELLRLEGMIAGSSTG
                     TLLSAALRYCREQTEPKRVLTFACDTGNKYLNKSFNDSWLADQGLLERESHGDLRDLI
                     ARPMGSGDMVTVTSEDNLITTYKRMRMFDISQLPVIDNGHIVGLIDESDILLGIYEDE
                     DNFQMPVSEVMVSNLETRPPSASIDDIVSLFKQDKVAIIADGDTFYGLITQIDLINHL
                     RQTMQ"
     gene            3444..>3892
                     /locus_tag="VIW27_03360"
     CDS             3444..>3892
                     /locus_tag="VIW27_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738735.1"
                     /translation="MDIQRLLTGLSNKIIVQLGVVLLAAVILIVLNFGILYPFYFENQ
                     LTDVGIVINGAIVALLLLGLGNILYHLLRYRREEATIAKFVGNAEALRPDLMEGLPAD
                     SIVARRHMTMRSIQKAHGEINHGALAAMLSADEATHFGLIRFINNIL"
BASE COUNT          802 a         1279 c         1141 g          670 t
ORIGIN      
        1 gagacgccgg ccaatccaac gctgaaactg accgatatcg cagcggtttc cgcggtcgct
       61 cagaaacacg gtattccgca cgtggtcgac aacacttttc tgacgccgtt ctaccagcgc
      121 ccgctcgaac tcggcgccga catcacactg cacagcacca ccaagttcat ggaaggccac
      181 aacatcagcg tcggcggctc gatcaccgcg gccaaccccg cgcacctcga gcagatcatg
      241 ttcgtgcgca actgcctcgg ctccaacatg acgccgatgg tcgcgttcta cacgctgcag
      301 ggtatcaaga ccatgagcac ccggctcgag gtgcagtcgg ccaacgcgct gaaggtcgcg
      361 caattcctcg agacccacgc caacgtcgaa cgcgtcgcct atcccgggct ggagtcgttt
      421 ccgcaaaagg aactcgccga ccgccaggcc agcggccacg gctcgatgct gtggttcgag
      481 gtcaagggcg gcgtcgccgc cggcaagaaa ctgatggata ctctcgaact gtggagcctg
      541 gcggaaaatc tcggctcggt cgagtcgctg gtcactcact cggtgaccat gacccacgct
      601 gacatgccgc gcgaggaacg cctcgccgcc ggcatcaccg acggactggt acgcctgtcg
      661 gccggcctcg aatcggccga cgacctgatc gcggatctca ggcaggcgct cgacgcactc
      721 tagccatgat cgtcaccagc atcgaatcct tcgccaccga gtacgtggcg ctggttcgag
      781 tgcgcaccga tgacggtgac gagggctggg gtcagatttc gccgtacatg gccgacctca
      841 gcgcacaggt gctgcaccgc caggtggcgc cgcatgtgct cggcatggac gccgccgaca
      901 tcgacgcggt taccgccgcg gtgctcgacc gcgagcacaa gttccagggc acctacctgt
      961 accgcgcgtt gtgcggcgtc gataccgcgc tgtgggacat tcgcggcaag cgcgccggca
     1021 agagcgtgca gcagttgctt ggcggcgtgc acgccgaatt cccggtgtat gcatcgagca
     1081 tgcagcgcga gatcactccc gaagccgaag ccgagcgcat gctcgcgttg cgcgagcagt
     1141 acggatatcg cgccttcaag ttccgcatcg gccgcgagtg cggtcacgac gccgaccagt
     1201 ggccgggacg caccgaggcg atcgtcaagc tgatgcgcga cacgctcggc gacgacgcca
     1261 ccctgctggt cgacgccaac agttgctaca gcccgcagaa ggcgatcgag atcgggcacc
     1321 tgctcaccga ctacggtatc agccatttcg aggaaccctg tccctactgg gaactcgact
     1381 ggacccggca ggtcacgcaa gcgctcgaca tcgacgtcac cggcggcgag caggacaaca
     1441 acatgatcgt ctggaaacag atgatcgata gtcgcgcggt cgacgtgatt cagcccgaca
     1501 tctgttacat gggcggcatc acgcgtacgc tgcaggtggc cgattacgcg caccgcgcgg
     1561 gcctgccgtg cacattgcac tcggccaacc tgtcattggt gacgctgttt tcgatgcact
     1621 tcatggcggc gatcgacaac cccggcaagt acgtcgagtt ctcgatcgag ggtctcgact
     1681 actacccgtg gcaggatcga atcttcacac ccggctacca gattcgcgac ggcaacgcgc
     1741 aaatttccgc ggcgccgggc tggggaatcg agatcaatcc cgaatggctt acactcgctg
     1801 actaccgggt aagttataat ggctcgcgct tctagcgcga acgaggagat ttgtgatgac
     1861 gatcgttaac tcgatactgg aactgatcgg caatacgccg attctcaaac tcagcaagtt
     1921 cgacaccggc ccgtgcgaac ttttcctgaa actggaatcg gaaaacccgg gcggctcgat
     1981 caaggaccgc atcgcagtgt gcatgatcga ggcggccgag aaagaaggca agatcgagcc
     2041 cggcggcacg atcatcgagg cgaccgccgg caataccggc ctcggactcg gcctggtggc
     2101 cgcgcaaaaa ggttacaagc tgatcctcgt catccccgac aagatgagcc ccgacaagat
     2161 tcgccatatc cgcgcgatgg gcgtcgacgt gcgcctgacg cgctccgacg tgcaaaaagg
     2221 ccatccggaa tactaccagg actatgccaa gcgcctggcc acggaaattc cgaactcgta
     2281 tttcatcgac caattctcca acccgaacaa cccgaagacc cacgagacca tgaccggccc
     2341 ggagatatac cggcagatgg acggcaggct cgacgcggtg gtggtcggcg tcggctcgag
     2401 cggcacggtg accggcctcg gcaagtattt ccaccgcgaa ctgccggacc tcgaaatggt
     2461 ggtcgccgat ccggtcggct cgatcctcgc cgacaccatc aataccggca acacgccgca
     2521 ggcgagttcg tggctggtcg agggcatcgg cgaggatttc ctgccgccga tctgcgacat
     2581 cagcctggtg aagaaagcct atgcgattcc cgacagggaa gccttcgcca cgcagcgcga
     2641 actgctgcgc ctcgagggca tgatcgccgg ctcgtcgacc ggcaccctgc tgtcggcggc
     2701 gctgcgctac tgccgcgaac aaaccgaacc gaaacgcgtg ctgaccttcg cctgcgatac
     2761 cggcaacaag tacctgaaca aatccttcaa cgattcctgg ctcgccgacc agggcctgct
     2821 cgagcgcgag agccacggcg acctgcgcga cctgatcgcg cgcccgatgg gcagtggcga
     2881 catggtcacg gtgacctccg aggacaacct gatcaccacc tacaagcgca tgcgcatgtt
     2941 cgatatctcg cagctgccgg tgatcgataa cggccatatc gtcggcctga tcgacgaatc
     3001 cgacatcctg ctcggcatct acgaagacga ggacaacttc cagatgccgg tcagcgaggt
     3061 catggtcagc aacctcgaga cccgaccgcc gtcggccagc atcgacgata tcgtgtcgct
     3121 gttcaagcag gacaaggtcg cgatcatcgc cgacggggat accttttatg gcctgatcac
     3181 gcagatcgac ctgatcaacc acctgcggca gacgatgcaa taagtggccg gctgccgcgg
     3241 ctgccgcttt cgggcgctgc gaccggcact gaaaatccgg agttttgcaa tgccttggcc
     3301 tgccctcctg gcgttggtcg gcggcccgtg ggaggtttct cggcgttgcg cagcggcgct
     3361 ttgacttcca ccggaaaacg ttatgtaatg cccggtatcg catcgtcgac tggtctgacc
     3421 gcacccaccc agagatcccc gcaatggaca ttcaacgcct gctcaccggt ttaagcaaca
     3481 agatcatcgt ccagcttggc gtcgtcctcc tcgccgcggt gatcctgatc gtgctgaatt
     3541 tcggcatcct ctacccgttt tacttcgaaa accagctgac cgacgtcggc atcgtcatca
     3601 acggcgccat cgtcgcgctg ctgttgctgg gactcggcaa catcctctat cacctgctgc
     3661 gctaccgccg cgaagaagcc acgattgcca aattcgtcgg caacgccgag gcgctgaggc
     3721 ccgacttgat ggaaggcttg ccggccgact cgatcgtcgc ccggcggcac atgacgatgc
     3781 gctcgatcca gaaggcccac ggcgaaatca atcacggcgc gctggcggca atgctgagcg
     3841 ccgacgaagc aacccatttc ggcctgatcc ggttcatcaa caatatcctg at
//
LOCUS       DATCZV010000153         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_116485, whole genome shotgun sequence.
ACCESSION   DATCZV010000153 DATCZV010000000
VERSION     DATCZV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116485"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VIW27_03365"
     CDS             complement(<1..282)
                     /locus_tag="VIW27_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5738736.1"
                     /translation="MTGHRLFPYLLILGAGVIWGATFSFALIATAGGAHPLAISAWQV
                     VLTAAFFLVICLLSRVRLFELRNLRHYIVLSLVGITLPNLLYYYAAPHLS"
     gene            538..1965
                     /locus_tag="VIW27_03370"
     CDS             538..1965
                     /locus_tag="VIW27_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5738737.1"
                     /translation="MDYLKFFINGDWITPLGTETIDVINPATEQPFAAVTAGTPADVD
                     RAVDAARRAFSDYSRWSVQQRLELLESIRSTYKRHFDAIAQAISDEMGAPVDLARGYQ
                     TKLGYAHIKSAIKALLNFRFEYTEEDMILRYEPIGVCGLITPWNWPMNQVAAKVMPAL
                     AAGCTMILKPSEESPLDAMIFTEVLREAGVPPGVFNLVNGYGPVVGEAMSGHAGIDMI
                     SFTGSTRGGIAVARAAADTVKRVAQELGGKSPNLILRDADLAAAVSEGVHYMMMNSGQ
                     SCNAPTRMLVPRDKLDEAVAAARAAAESIGVDDPAKPGEHLGPVVNKTQFDKIQGLIQ
                     QGIDEGAALVTGGTGRPEHLPTGYYVKPTVFADVDNGMTIAREEIFGPVLVIIGYDDE
                     DDAVAIANDTDFGLAAYVCSNEMERARAMARRLRAGQVAINYIGGTSDTPFGGYKQSG
                     NGREKGRWGLEEFLELKAITGSVTS"
     gene            1986..>2119
                     /locus_tag="VIW27_03375"
     CDS             1986..>2119
                     /locus_tag="VIW27_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5738738.1"
                     /translation="MRFDFSGKRVLITGGTSGIGEATALAFAGAGARVVISGRDTRRA
                     "
BASE COUNT          414 a          655 c          719 g          331 t
ORIGIN      
        1 ggacaggtgg ggcgccgcgt aatagtagag caggttcggc agggtgatgc cgaccagcga
       61 cagcacgatg taatgtcgca ggttgcgcaa ctcgaacagg cgcacccgcg agagcaggca
      121 gatgacgaga aagaatgccg ccgtcaacac cacttgccag gcggagatgg cgagtgggtg
      181 ggcgccgccg gcggtggcga tcagcgcaaa agaaaaggtc gctccccaga tgacgcccgc
      241 gccgagaatc agcaggtagg gaaacagccg atgtccggtc ataatacctt ttcgaggttg
      301 cgcgggaagg acgaacaggc cgattatagg ggccgatcac cgcacagggc acgcggtttt
      361 tttgcgggac cgggtcgcgc gcgcgcgcaa acggtgaatt atcgccgatc agccggtatg
      421 ggaccggcgc cgccacggtt cgcatcggcg gctgaaaaaa tccttgcacg ggccattgct
      481 tcgcggcaaa ttgtcccagg ttaaccacta ttcgcttcga cgacagggaa caggcatatg
      541 gattacctca agttttttat caatggcgac tggatcactc cgctcggcac cgagaccatc
      601 gacgtcatca accccgccac cgaacagccg ttcgccgcgg tcaccgcggg aacgccggcg
      661 gatgtcgatc gcgcggtcga tgccgcgcgc cgagcgttca gcgactactc gcgctggagc
      721 gttcaacagc gtctcgaact gctggaaagc attcgcagca cctacaagcg gcatttcgac
      781 gctatcgcgc aggcgatcag cgacgagatg ggcgcaccgg tcgacctggc ccgcggctac
      841 cagaccaagc tcggctacgc gcacatcaag agcgcgatca aggcgctgct gaatttccgc
      901 ttcgagtaca ccgaggagga tatgatcctc cgctacgagc cgatcggcgt ctgcggcctg
      961 atcacgccgt ggaactggcc gatgaaccag gtcgccgcca aggtcatgcc ggcgctggcc
     1021 gccggttgca cgatgattct gaagccgagc gaggaatcgc cgctggacgc gatgattttc
     1081 accgaggtat tgcgcgaggc cggcgtgccg ccgggtgtct tcaatctcgt caatggttac
     1141 ggcccggtgg tcggcgaggc catgtcgggc catgcgggga tcgacatgat atcgttcacc
     1201 ggctcgacgc gcggcggcat cgcggtcgcc agggcggccg ccgacaccgt caagcgggtc
     1261 gcccaggaac tcggcggcaa gtcgcccaat ctcattttgc gcgacgccga cctcgccgcg
     1321 gcggtcagcg agggtgtgca ctacatgatg atgaacagcg gacagtcgtg caacgcgccg
     1381 acccgcatgc tggtgccgcg cgacaagctc gacgaggcgg tcgccgcggc cagggcggcc
     1441 gcggagagca tcggcgtcga cgacccggcg aagcctggcg agcatctcgg cccggtggtc
     1501 aacaagaccc agttcgacaa gattcaggga ctgatccagc agggaatcga cgagggcgcg
     1561 gcgctggtta ccggcggcac cgggcgcccc gagcacctgc cgaccggtta ctatgtcaag
     1621 ccgaccgtgt tcgccgatgt cgacaacggc atgaccatcg cgcgcgagga gatattcggt
     1681 ccggtgctgg tcatcatcgg ctacgacgac gaggacgacg cggtggcaat cgccaacgat
     1741 accgatttcg ggctcgccgc ctacgtttgc tcgaacgaga tggagcgcgc cagggcgatg
     1801 gcgcgccgcc tgcgcgccgg tcaggtggcg atcaactata tcggtggcac ctccgatacc
     1861 ccgttcggcg gttacaagca gtccggcaac ggccgcgaaa agggcaggtg gggactcgag
     1921 gaatttctcg aactgaaggc gatcaccggc tcggtgacga gttgaccgcg gtgagcagcg
     1981 cctcgctgcg cttcgacttt agcggcaagc gagtgctgat caccggcggt acctcgggca
     2041 tcggcgaggc caccgcgctg gcttttgccg gggccggcgc gcgggtcgtg atcagcgggc
     2101 gcgacaccag gcgggccag
//
LOCUS       DATCZV010000154         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_116561, whole genome shotgun sequence.
ACCESSION   DATCZV010000154 DATCZV010000000
VERSION     DATCZV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116561"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VIW27_03380"
     CDS             complement(<1..826)
                     /locus_tag="VIW27_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738739.1"
                     /translation="MSVGVRVLIAVFIVLTMGLSSTRVLDDYVDDYTDEALKNAALTY
                     ATARGINALVSMMQSSEIEAGVGVVSGAITVGELLDPLNDMIERFSTIMTWVLASLAA
                     QKVLLLIASHKLFLYLVAALGILALLALFNGTPRTRNLVFRLFLVAVFVRFSLGLAVA
                     LNSGADLLFLDQQLQENDAEIARFQDKVIGIDGDQALDSSSVRNSALRFWQGLSLDEL
                     ERRISEGIESFINLVAIYLLKTILFPLGFFYLAFHLVRLLWRFDLGPAAPRLTADRP"
     gene            complement(833..1174)
                     /locus_tag="VIW27_03385"
     CDS             complement(833..1174)
                     /locus_tag="VIW27_03385"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010371199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5738740.1"
                     /translation="MSSLSQQECEACRADAPKVSDEELRELMPQIPDWAATVVDGVMQ
                     LTREYSFANFEQAIAFTNRVGEIAESAGHHPAILTEWGKVTVNWWTHKIRGLHRNDLI
                     MAARTDELYGG"
     gene            complement(1254..2153)
                     /locus_tag="VIW27_03390"
     CDS             complement(1254..2153)
                     /locus_tag="VIW27_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5738741.1"
                     /translation="MSDQTANAPTCTAASGAIDLLIEPKARDLGGFSVRRVLPSPRRR
                     MVGPFIFFDHMGPAEFPPGEGIQVRPHPHIGLATVTYLFEGEIFHRDNLGYQQPIRCG
                     AVNLMTAGSGIVHSERAGDDLHTTSRLHGIQSWMALPLDDEECTPDFTHYPADSIPGF
                     EHQGASIRVIIGSAFGHQSPVRQFAPTLYLECRLPQGARLELPRETEELACYVVAGEV
                     RIDEHKIGDGLMAVACDDADLRIEATTDSLLMIIGGASLGKRHIWWNFVSSSKQRIEQ
                     AKEDWREGRFAGVPGDDEFIPLP"
     gene            complement(2210..3415)
                     /gene="nhaA"
                     /locus_tag="VIW27_03395"
     CDS             complement(2210..3415)
                     /gene="nhaA"
                     /locus_tag="VIW27_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY5738742.1"
                     /translation="MIPTPEPASLVNMMRQFIRLEAAGGILLLITAVLAMLIANSPLA
                     SLYGSLLDTTIAVQIGTLEIRKPLLLWVNDGLMAVFFFLVGLEIKREVLEGELSSVSQ
                     IVLPAVGALGGMLVPAAIYVWLNWNDSGALDGWAIPVATDIAFALALIGVFGARVPTA
                     LKVFLLTLAIFDDLAAIVIIALFYSGDLSIGALLFGGAALAAAVIINRLGITRVSAYI
                     LIGIVLWIAVLKSGVHATLAGVLIAFCVPMRDAEGNSPLRSLEHDLHTPVAFFILPIF
                     AFANAGLALGGMAVADILHPVTLGVMLGLLLGKPVGVVLFVGLAVAMRLVSLPANVNW
                     LQVLGVGFACGIGFTMSLFIAGLAFEHGGGDYFAGDRLGILLGSILSAAAGYLVLQFS
                     LPKGAAAGE"
     gene            3551..>3893
                     /locus_tag="VIW27_03400"
     CDS             3551..>3893
                     /locus_tag="VIW27_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738743.1"
                     /translation="MSKSRFVLKFIGWTLAALLALVVIVRLSLAPLVSGAIVGFFADQ
                     GVEARIDDLSFELADGKATLGGLSAAVNGRTMLALGQLELGWSWDALTENRLRIESLR
                     LDGLDLEILQGE"
BASE COUNT          797 a         1235 c         1204 g          657 t
ORIGIN      
        1 gggggcgatc ggcggtgaga cggggggcgg cggggccgag atcgaagcgc cataacagtc
       61 tgacgaggtg aaaggcgagg taaaaaaagc cgagcggaaa gaggatggtc ttcagcaggt
      121 agatcgcgac caggttgatg aagctctcga tgccttcgct gatgcggcgc tccagttcgt
      181 cgaggctcaa cccctgccag aagcgcagcg ccgagttgcg cacactgctg ctgtccagcg
      241 cctggtcgcc atcgataccg atgaccttgt cctgaaagcg ggcgatctcg gcgtcgtttt
      301 cctgcagctg ttgatcgaga aacaacaggt cggcgccgct gttgagcgct accgcgaggc
      361 ccagcgaaaa gcgcacgaag accgccacca ggaacagcct gaaaaccagg ttgcgggtac
      421 gcggcgtgcc gttgaacaat gccagcaacg cgagtatgcc gagcgccgcg accaggtaaa
      481 ggaacagctt gtgggaggct atcagcagca gcaccttttg cgccgccagc gaagccagca
      541 cccaggtcat gatggtcgag aagcgctcga tcatatcgtt caacggatcg agcaactcac
      601 cgaccgtgat cgcacccgag accacgccga ccccggcctc gatctcactg ctttgcatca
      661 tcgataccag cgcattgatg ccgcgcgcgg tggcataagt cagcgccgcg tttttcagcg
      721 cctcgtcggt gtaatcgtcg acatagtcgt cgagcacccg ggtgctcgac aggcccatcg
      781 tcagcacgat gaagaccgca atcagcactc ggacgccaac gctcatcgcg ggctaaccgc
      841 cgtagagttc gtcggtgcgc gcggccatga tcaggtcgtt gcggtgcagg ccgcgaatct
      901 tgtgcgtcca ccagttgacg gtgaccttgc cccattcggt gaggatcgcc gggtgatgcc
      961 cggccgactc tgcgatttcg ccgacgcgat tggtaaaagc gatagcctgt tcgaaattcg
     1021 caaagctgta ctcgcgcgtc aactgcatca caccgtcgac gacggtcgct gcccagtcgg
     1081 gaatctgcgg catcaactcc ctgagttcct cgtcgctcac cttcggcgca tcggcgcggc
     1141 aggcctcgca ttcctgttgg ctcaagctgc tcatctggat actccgcttg cgattgactg
     1201 agcgcctagt ttaaagcgcg caccggcatt agcaaatccc ggccagtccg gcatcagggc
     1261 agcggaatga actcgtcgtc gccgggcacc ccggcgaagc gcccctcgcg ccagtcttct
     1321 ttcgcctgct cgatgcgctg cttcgaactg gaaacgaaat tccaccagat atgccgcttg
     1381 ccgaggctgg cgccgccgat gatcatcagc aggctgtcgg tcgtcgcttc gatgcgcaga
     1441 tcggcatcgt cacaggccac cgccatcagg ccatcaccga tcttatgctc gtcgatgcgc
     1501 acttcgccag caaccacgta gcaagccagt tcctcggtct ctctcggcag ttcgagcctg
     1561 gccccctgtg gcaggcggca ctcgaggtaa agcgtcggcg cgaattgccg gacgggcgac
     1621 tggtggccga aagcggagcc gataattacc cgaatcgatg cgccctggtg ttcgaatccg
     1681 ggaatcgaat ccgccgggta atgggtgaaa tccggcgtgc attcctcgtc gtcgagcggc
     1741 agcgccatcc acgattgaat gccgtgtagc cgcgaggtgg tatgcagatc gtcgccggcg
     1801 cgttccgaat gcacgatgcc gcttcccgcg gtcatcaggt tgaccgcgcc gcagcgtatc
     1861 ggctgctggt agccgagatt gtcgcggtga aagatttcgc cctcgaacag gtaagtcacc
     1921 gtggccagcc cgatgtgcgg atgcgggcgc acctgtatgc cctcgccggg cggaaattcc
     1981 gccggtccca tgtggtcgaa aaagatgaac ggcccgacca tgcgccgtcg cggcgatggc
     2041 agcacgcgac gcacgctgaa tccgcccagg tccctggcct tgggctcgat cagcagatcg
     2101 atcgctcccg aggcagccgt gcaggtcggt gcgttggcag tctggtcact cattatcaca
     2161 ctcctgggta tatgatcgac cgccactggc cggtcgacgg gtcctcgagc tactcgcccg
     2221 cagcagcgcc cttcggcagg ctgaactgca ggaccaggta gccggccgcg gccgacagga
     2281 tcgagcccag taaaatgccg aggcgatcac cggcgaagta gtcaccgccg ccgtgctcga
     2341 aggcgaggcc ggcgataaac aggctcatcg tgaacccgat accacaggca aagccgaccc
     2401 cgagtacctg cagccagttg acattggccg gcaggctgac caggcgcatc gccaccgcca
     2461 gaccgacgaa cagcacgact ccgaccggct tgccgagcag caggccaagc atcacgccga
     2521 gggtgaccgg gtgcaggata tcggcgacgg ccatgccgcc gagtgccagg ccggcgttgg
     2581 caaaagcgaa gatcggcaga atgaagaagg ctaccggggt atgcaaatcg tgctccagcg
     2641 agcgcagtgg cgaattgccc tcggcgtcgc gcatcggcac acaaaacgcg atcaacaccc
     2701 cggcgagcgt cgcgtgcacg ccggacttca gaaccgcgat ccacagcacg ataccgatca
     2761 ggatataggc cgacacgcgg gtgatgccga ggcgattgat gattaccgcg gcggccagcg
     2821 cagcgccgcc gaacaacagc gcgccgatcg acagatcgcc cgagtaaaac agcgcgatga
     2881 tgacgatcgc ggcgaggtcg tcgaatatcg ccagcgtcag caggaaaacc ttgagcgcgg
     2941 tcggtacgcg cgcgccgaac acgccaatca gcgccagcgc gaaggcgata tcggtggcca
     3001 ccgggatcgc ccagccatcg agcgcgccgc tgtcgttcca gttcagccag acataaatcg
     3061 ccgccggtac cagcatgccg ccgagcgcac cgaccgcggg cagcacgatc tgcgataccg
     3121 aagacagctc gccttccagc acctcgcgct tgatttccag gccgacgaga aaaaagaata
     3181 ccgccatcag gccgtcgttg acccatagca acaacggttt gcgaatttcc agcgtaccga
     3241 tttgcaccgc gatcgtggta tccagcaggc tgccgtaaag cgacgccagc ggcgaattgg
     3301 cgatcagcat cgccagcacc gcggtaatca acaacagtat gccgccggcc gcctcgaggc
     3361 ggatgaactg gcgcatcatg ttgaccaggg acgctggttc cggagtaggt atcataggga
     3421 gcacagttta attatcgctg ggcaacatgc aatcccgacg atcgaacgcg aattaatccg
     3481 aagttgagga ctccgcgtaa ctagtgcgtt attcttacac ccgactcaaa acatcgccaa
     3541 gcccagaccc atgtcgaaat caagatttgt cctcaagttc atcggctgga cgctggccgc
     3601 gctgctggcg ctggtggtaa tcgtgcgttt gtcgctggcg ccgctggtca gcggcgcgat
     3661 cgtcggcttt ttcgccgacc agggtgtcga ggcgcgcatc gacgatctga gcttcgaact
     3721 cgccgacggc aaggcgacgc tcggcggcct gtctgcggcc gtcaacggca ggacaatgct
     3781 cgccctcggc cagctcgagc tcggctggtc gtgggacgcg ttgaccgaga accggctgcg
     3841 catcgagtcg ctgcggctcg acggcctcga tctcgaaatc ctgcagggcg agg
//
LOCUS       DATCZV010000155         6228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_434522, whole genome shotgun sequence.
ACCESSION   DATCZV010000155 DATCZV010000000
VERSION     DATCZV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434522"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VIW27_03405"
     CDS             <1..296
                     /locus_tag="VIW27_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003396053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5738744.1"
                     /translation="SFAIDDFGTGYSSLNYLHRLPVDELKIDQSFVRNISGSSSNAVI
                     IDTIIMMAQQLDLKVVAEGIETRAELDYLLARNCDYYQGFYFSRPMPPELFTG"
     gene            complement(293..3430)
                     /locus_tag="VIW27_03410"
     CDS             complement(293..3430)
                     /locus_tag="VIW27_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006010085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5738745.1"
                     /translation="MTRYFAAHPTAANILMFIILLLGIAALSGLNKETFPEIELYQVQ
                     VTVNYPGASASEVEEGICNRLEDATDGISFLEERSCQARDNLGIMVLEMQEIGNLQKF
                     IDDINSAVDGITDFPDDAEEPLIDELGRTVAVVSVAIAAQLSQSELKALAEYYRNRLL
                     ALSEVPMVDVSGFSTHEFSVLIKAETLRRYRLSIQDIAELIRNQAVDLPAGVLKGGQR
                     WYQIRVENERRSVEELADLVVLNDGRGGQLRLGDIATIADEFTDREVRVELDGRPAAL
                     LQVRKNSGDDTLTVYDAVSDFVAAENARLPASTRLVITQDAASIVKDRLNMLIRNGWQ
                     GLLLATLSLFLFFSWRYTFWVALGLPVSFIGGLMVMNLFGISINMISMVALLMAIGIL
                     MDDAIVISESIESEHRAGKPPLQAAVDGIAKVGRGVLASYLTSAILFGSLLFLSGHMG
                     QVLGVLPVVLLSVLTISLVEAFLILPHHLVNSLRHHRNELSSGWRKGFERRFERLRDG
                     VGRVADLAINYRYLTVGCALGLLVVSISLIPAGLIKFKAFPDLEGNLLQARLIMPQGT
                     PFERTEEVVAGLLESLAEARRQLPPEPDGELIRHVQVFYSDNADAGEQGAHLATVSLD
                     LLDSGLRRTSLNELRRRWLEATGSVPDAVSIQFKEPVLGPAGQAISIRLQHDDLRQLS
                     NASWELQTWLNGYPGVSNLMDDLRLGKPQFTVSLLPGSLGSGLNARQLAQQLRAAYQG
                     VKVDDVYRGREAYEINVRLDTDRDNALRDFEQLGVFNDQGVDIPLSAIARIEEKREFS
                     HITRINHQRTATISGDIDADIANTNEIINDTRERFLGGLQQRYPGLFVSLEGEVKNSR
                     ETNQSVLLGFALGIFGVYFLLSFQFRNYREPLVVLINIPLALIGVIWGHKLMGLEITL
                     PSIIGFVSLSGIVVNDSILLVEFVKRRSLEGMTLHAAAGQAVRDRFRAIFLTSVTTVA
                     GMLPLLTETSLQAQVLVPLVASVVFGMISSTLLLLLVLPSAYAIMEDLGIRENDDQAR
                     AFIDTMKPGAASETA"
     gene            complement(3430..4791)
                     /locus_tag="VIW27_03415"
     CDS             complement(3430..4791)
                     /locus_tag="VIW27_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY5738746.1"
                     /translation="MKLQARDGFALLLALAVAAAIAVVLVNSRVPLEHVAVEMPRRAV
                     EALVVREIDFRSRITAYGNVEPAITLDSMAEVSGKISYVHPNLKSGETIPAGTLVVSI
                     EAEDYELSLQQTREDLKASRSALAELEAEVKSTRRSLELARENLVVGEAELARIRDVY
                     RQQLIAKSTLDAEEQKVIQLRQQVEDLQGRIDSQASRRQSIEAQIARAEQEVQNRATI
                     LGRTGTTLPFDARIGSVSIDENEFVTVGSPLFEAIDLRGVEISAQLPLVSMRQLISHL
                     EDREVALQQFVQTGGRINESLGLSARVRLVNGMPEAVWDARVLRISEAIDETRQTLGV
                     VVGVDDPYEKIIPGRRPPLLKGMYTAVDLFAPQRSALVIPRRALHEGRVYLADADDRL
                     EIRAVDVQLTQGELAVIRGGLEIGERVIVNDLVPVIAGMPLEVNLVAGIEEDLRRRAL
                     GEI"
     gene            4860..>6228
                     /gene="pyk"
                     /locus_tag="VIW27_03420"
     CDS             4860..>6228
                     /gene="pyk"
                     /locus_tag="VIW27_03420"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5738747.1"
                     /translation="MTRYSPRRVAATVIAPAFAAIIGRLSLLFARLTLIQRKKFRKTK
                     VIATIGPACNSLPVLKAMIEAGMNVARLNMSHGDEASHAATVASIREAAAAAGATIAI
                     MVDTRGREIRSGRVDNGQVLLNAEQAFTIYADDRVGDASGVSISLKSLDRHARPGQRV
                     LIDDGQIELQVESVEPGQVHCRVVTGGVLRNNKGVNLPDTTAALNDIEYDTTRELRFA
                     VDQQVDYLAASFVRNAAEVESITAQLRELGAEIPIIAKIENREGVQNIEEIIEASAGI
                     MVARGDLGVELDMGEGPTIQKRIIRATVANGKPVITATQMLDSMERNPRPTRAEASDV
                     ANAIFDGSSAVMLSGETASGSRPVEAVATMVHLALEAEQNLREFGVLQQIKPHPSNDV
                     TEAVAQAAITMANHLNSAAIIVMTETGFSARLVSKYRPDCPIVAITFTEEVVRRLALS
                     WGIYGF"
BASE COUNT         1186 a         2061 c         1881 g         1100 t
ORIGIN      
        1 tcagcttcgc gatcgacgat ttcggcaccg gttactcgtc gctgaattac ctgcaccggt
       61 tgccggtcga cgagctgaag atcgaccagt ctttcgtcag aaatatttcc ggatcgtcga
      121 gcaacgcggt gatcatcgac accatcatca tgatggcgca acagctcgac ctcaaggtag
      181 tcgccgaagg catcgagacg cgcgccgagc tcgactacct gttggcacgc aactgcgact
      241 attaccaggg cttttacttc tcgcggccga tgccgccgga actgtttacc ggttaagcgg
      301 tttcgctcgc agcgcccggt ttcatggtgt cgatgaatgc ccgcgcctgg tcatcgttct
      361 cgcgaatacc gagatcttcc atgatcgcgt aggcggacgg cagcaccagc aacaatagca
      421 gcgtcgacga aatcatgccg aacaccacgc tggcgaccag cggcaccagc acctgcgcct
      481 gcaggctggt ttcggtcagc agcggcagca tgccggcgac ggtggttacc gaggtcagga
      541 aaatcgctcg aaaacgatcg cgcaccgcct gcccggcggc ggcatgcagc gtcatgccct
      601 cgaggctgcg gcgcttgacg aactcgacca gcaggatcga atcgttgacc acgatccccg
      661 acagcgagac aaatccgatg atgctcggca gcgtgatttc gagccccatc agcttgtgac
      721 cccagatcac gccgatcaac gccagcggaa tattgatcaa caccaccagc ggctcgcggt
      781 aattgcgaaa ctggaagctc aacaggaagt aaaccccgaa gataccgagc gcgaagccga
      841 gtagaaccga ctggttggtc tcgcggctgt tcttgacctc gccctcgaga ctgacgaaca
      901 gccccggata gcgctgctgc aggccgccga gaaagcgctc gcgggtatcg ttgataattt
      961 cattggtatt ggcgatgtcg gcatcgatat cgccgctaat ggtggcggta cgctggtgat
     1021 tgatgcgcgt gatatgcgaa aactcgcgct tttcctcgat acgggctatg gcgctcaatg
     1081 gaatatcgac gccctggtcg ttgaacacgc cgagctgctc gaagtcgcgc agcgcattgt
     1141 cgcgatcggt atccagcctg acgttgattt cgtaggcctc ccggccgcgg tagacatcgt
     1201 cgaccttgac cccctggtag gcggcccgca gttgctgcgc cagctgccgg gcattgagtc
     1261 ccgagcccag cgacccgggc agcaggctga ccgtaaactg cggcttgccg agccgcaggt
     1321 cgtccatcag gttggaaacc cccgggtagc cgttgagcca ggtctgcaac tcccacgaag
     1381 cgttcgacaa ctgccgcagg tcgtcgtgct gcaggcgaat cgagatcgcc tggcctgccg
     1441 ggccgagcac aggctccttg aactggatcg agaccgcgtc cggcaccgag ccggtggctt
     1501 ccagccagcg acgacgcaat tcgttcagcg acgtgcggcg caaccccgaa tcgagcaggt
     1561 cgagactgac cgtggccagg tgtgcgccct gttcgccggc atcggcgttg tcgctgtaga
     1621 acacctgcac atgccggatc agctcgccat cgggttccgg cggcaactgc cgccgcgcct
     1681 ccgcgagact ctccagcagc ccggcgacga cttcctcggt acgctcgaag ggcgtcccct
     1741 gcggcatgat caggcgcgcc tgcagcaggt tgccctcgag gtccggaaag gccttgaact
     1801 tgatcagccc cgccgggatc aggctgatac tgaccaccag caggcccagc gcgcagccga
     1861 cggtcaggta tcggtagttg atggcgaggt cggccactcg cccgacaccg tcccgcaatc
     1921 gttcgaagcg acgctcgaaa cccttgcgcc agcccgacga cagctcgttg cggtgatgcc
     1981 gcagcgagtt caccaggtga tgcggcaata tcaaaaacgc ctcgaccagg ctgatggtca
     2041 gcaccgacag cagtaccacc ggcagcacgc cgagcacctg ccccatgtgt ccgctcagga
     2101 acaacaggct gccgaacagg atagccgagg tcaggtacga cgccagcacg ccgcggccga
     2161 ccttggcgat gccgtctacc gcggcctgca gcggcggctt gccggcgcga tgctcgctct
     2221 cgatactctc cgaaatgacg atcgcgtcgt ccatcaggat accgatcgcc atcagcagcg
     2281 cgaccatcga gatcatgttg atgctgatgc cgaacaggtt catcaccatc aatccaccga
     2341 tgaaagacac cggcaagccg agcgccaccc agaaggtgta acgccagctg aagaacagga
     2401 acagcgacag cgtggccagc agcaatccct gccagccgtt gcggatcagc atgttgaggc
     2461 ggtccttgac gatggaggcc gcatcctggg taatcaccag ccgggtcgac gcgggcagcc
     2521 gggcattttc ggcggccacg aagtcgctga ccgcgtcgta aacggtcagc gtgtcgtcac
     2581 cgctattctt tctgacctgc agcaaggcgg ccggccgccc atcgagttcg acgcgcactt
     2641 cccggtcggt gaactcatcc gcgatggtgg caatatcgcc gagacgcagc tgcccgccgc
     2701 gaccgtcgtt gagcaccacc aggtcagcca gctcctcgac gctgcggcgc tcgttttcga
     2761 cgcggatctg gtaccagcgc tggcctccct tgagcactcc cgccggcagg tcgaccgcct
     2821 gattgcgaat cagttcggcg atatcctgaa tgctgagccg atagcgccgc agggtttcgg
     2881 ccttgatcag cacgctgaac tcgtgggtcg aaaaaccgct gacatcgacc atcggtacct
     2941 cggacagcgc cagcagccgg ttgcggtaat attccgccag cgccttgagt tcggattgcg
     3001 acaactgtgc cgcgatcgcc acgctgacca cggcgacggt ccggcccagc tcatcgatca
     3061 gcggttcctc ggcatcgtcc gggaagtcgg tgatgccgtc caccgccgag ttgatgtcgt
     3121 cgataaactt ctgcaggttg ccgatttcct gcatctcgag caccatgatg ccgaggttgt
     3181 cgcgcgcctg gcagctgcgt tcctcgagaa aactgatgcc gtcggtggcg tcctcgagcc
     3241 ggttgcagat gccctcctct acctccgagg cgctggcgcc ggggtaattc acggtcacct
     3301 gcacctggta aagctcgatc tcgggaaagg tttccttgtt caggccggac aaggcggcga
     3361 tgcccagcag caggataatg aacatcagga tattcgcggc cgtcggatgg gccgcaaaat
     3421 aacgcgtcat caaatctctc cgagcgcccg gcgccgcaga tcctcctcga tcccggcaac
     3481 caggttcacc tcgagcggca tgcccgcgat caccggcacc aggtcgttga cgatgacgcg
     3541 ctcgccgatc tcgaggccgc cgcgaatgac cgccagttca ccctgggtca gctgtacgtc
     3601 gaccgctcga atctcgagcc gatcgtcggc atcggccaga taaacccggc cttcgtgcag
     3661 cgcgcggcgc ggtatcacca gcgccgagcg ctgcggtgca aacaggtcga ccgcggtata
     3721 catgcccttg agcagcggcg gtcgccggcc cggaatgatc ttttcgtagg gatcgtcgac
     3781 accgaccacc acacccagcg tctgccgcgt ctcgtcgatc gcctcgctga tgcgcagcac
     3841 ccgcgcatcc cagaccgctt ccggcatgcc attcaccagg cgcacccgcg cgctcaaccc
     3901 gagactctcg ttgatgcgcc cgccggtctg cacgaactgt tgcagcgcga cttcgcgatc
     3961 ctcgaggtga ctgatcagct ggcgcatcga gaccagcggc aactgggcgc tgatctccac
     4021 gcccctcaga tcgatggctt cgaacaacgg cgaaccgacg gtgacgaatt cattctcgtc
     4081 gatactgacg ctgccgatgc gcgcatcgaa gggcagcgtg gtgccggtac ggccgaggat
     4141 cgtggctcgg ttctgcacct cctgctcggc gcgcgcgatc tgcgcctcga tcgactgccg
     4201 gcggctggcc tggctatcta tcctgccctg caggtcttcc acctgctggc gcaactgaat
     4261 gaccttttgc tcctcggcgt cgagcgtgga tttggcaatc agctgctgcc gataaacatc
     4321 ccgaatgcgc gccagttccg cctcgccgac taccagattc tcgcgcgcca gctcgagcga
     4381 gcgacgggtc gacttgacct cggcctcgag ttcggccagc gccgagcggc tcgccttgag
     4441 gtcttcgcgg gtctgctgca gcgacagctc gtaatcctcg gcctcgatgc tcacgaccag
     4501 ggtaccggcc ggaatggtct cgcccgactt gagattggga tgcacgtagc tgatcttgcc
     4561 gctgacttcg gccatgctgt cgagggtaat cgccggctcg acattgccgt aagcggtgat
     4621 ccgcgaccga aaatcgatct cgcgtaccac cagcgcctcg acggcgcggc gcggcatctc
     4681 caccgcgacg tgctccagcg ggactcgact gttcaccagc acgaccgcta tcgccgcggc
     4741 cacggcgagc gcgagcaata gcgcaaatcc gtctctcgct tgcaatttca tggttttctg
     4801 gtccctgcga atattccttt acgggcctgc gccgcgattc taacttgcca gcgccgacga
     4861 tgaccagata ttccccacgc cgcgttgcgg cgacagtgat tgcgccggcg ttcgcggcta
     4921 taattggccg gttatcatta cttttcgcga gacttaccct gatccagcgc aagaaattca
     4981 gaaagaccaa ggtcatcgcc accatcggcc cggcctgcaa ttcactgcca gtgctgaagg
     5041 cgatgatcga ggccggcatg aacgtcgcac ggctcaacat gtcgcacggt gacgaggcat
     5101 cgcatgcggc gaccgtcgcc agcattcgcg aggccgccgc cgcggccggg gcgacgatcg
     5161 cgatcatggt cgatacccgc gggcgcgaga tccgcagcgg cagggtcgac aacggccagg
     5221 tgttgttgaa tgccgagcag gcgttcacga tctacgcgga cgatcgcgtc ggcgacgcca
     5281 gcggcgtatc gatctcgctg aagagcctcg accggcatgc gcggccgggg cagcgggtgc
     5341 tgatcgacga cgggcagatc gagctgcagg tcgagtcggt cgagccggga caggttcact
     5401 gtcgcgtggt caccggcggt gtgctgcgca acaacaaggg tgtcaacctg ccggatacca
     5461 ccgcggcgct caacgacatc gagtacgaca ccacgcgcga actgcgcttt gccgtcgacc
     5521 agcaggtcga ctacctggcc gcgtcgttcg tgcgcaacgc cgccgaggtc gagagcatca
     5581 ccgcacaatt acgcgagctc ggcgccgaaa tcccgatcat cgccaagatc gaaaaccgcg
     5641 aaggggtgca gaatatcgag gaaatcatcg aagcctcggc cggcatcatg gtggcgcgcg
     5701 gcgacctcgg cgtcgaactc gacatgggcg aaggcccgac gatccagaaa cgcatcattc
     5761 gcgccacggt cgccaacggc aaaccggtga tcaccgccac acagatgctc gattcgatgg
     5821 aacgcaaccc gcggccgacc cgcgccgagg ccagcgacgt cgccaacgcg atcttcgacg
     5881 gcagctcggc ggtcatgctg tcgggagaga ccgcttccgg ctcgcgcccg gtcgaggcgg
     5941 tcgcgaccat ggtccacctg gcgctcgaag ccgagcaaaa cctgcgcgag ttcggcgtct
     6001 tgcagcagat caagccgcac ccgtcgaacg acgtcaccga ggcggttgcc caggctgcga
     6061 tcaccatggc caaccacctg aattcggcgg ccatcatcgt catgaccgag accggctttt
     6121 ccgcgcgtct ggtttccaag tatcggcccg actgcccgat cgtcgccatc actttcaccg
     6181 aggaagtagt gcgccggctg gcgctgagct ggggtatcta cggttttc
//
LOCUS       DATCZV010000156         7058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_593120, whole genome shotgun sequence.
ACCESSION   DATCZV010000156 DATCZV010000000
VERSION     DATCZV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593120"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VIW27_03425"
     CDS             <1..845
                     /locus_tag="VIW27_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5738748.1"
                     /translation="AGGSGWDVFVPTNYTITTYVGEDLIEPLDLSKLSNFKADAFDPR
                     FSDAGTVDGKLYAVPKNWGTTGYVVDNAHNKGARMTSWKDFWDRTKTDFSGRTMVHDY
                     QLTTIGNALKYFGYSFNSVDPKELADAEKLLLEVKPHLFAINSDYQPPLRNGDAWMSM
                     CWTGDGKQLNSDIPTMEYVLGVEGGEIWSDYYAVPRGAEHREAGYALINFMLDPAINA
                     REVLAHGYPVADSRTNALLPKELLEDPILYPAQELLDALEFGAAATLTDPNRAELLAR
                     FKSA"
     gene            867..1718
                     /locus_tag="VIW27_03430"
     CDS             867..1718
                     /locus_tag="VIW27_03430"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738749.1"
                     /translation="MKSSWAHRLLLLPASLWLLVMLVLPLLVVFVFSFGERAPAGGYI
                     PAFSFDNYLNLPARLTAFKNTMIMAPLGTLAALLIAYPLAYYLAVRANPRIKTTLLVM
                     IIIPFWTSILIRIYAWIYILGGRGIPQLLEWIGIEGVRLINTPTAVLIGIIYGYLPLM
                     VFPIYVSLEKFDKRLLEASEDLGASPLRTFRDVTLPLSLPGIATGCMLVFILLMGEFL
                     IPALLGGGKVFFVGNALVDLFLQSRNWTYGSAVATALVVIMLVTVTVYMKLIMRGAGQ
                     REDVSLL"
     gene            2115..2933
                     /locus_tag="VIW27_03435"
     CDS             2115..2933
                     /locus_tag="VIW27_03435"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013067975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738750.1"
                     /translation="MRLYAIVIYLFLYLPIGIIVLFSFNAGRHASDFQGLSVKWYGKV
                     LSNPFAMDALATSLTVAFVTALLASLFGTMAALGLQQVRGRLRALIDAVIYIAIMIPG
                     IVIGIATLIALVTAFDFLNPALQASWPAWLGDAPRLRMGRVSLVAAHTMFTMALVIVI
                     VRARIAGMDRTLIEASADLYATPMGTFRQVTLPQIFPAVLAGFLLSFTFSFDDFIIAF
                     FVAGSETTLPIYVFSSIRRGVTPEINAIGTMVLAFSLTLLAAAQVMLRRRAQPN"
     gene            3315..4307
                     /gene="dctP"
                     /locus_tag="VIW27_03440"
     CDS             3315..4307
                     /gene="dctP"
                     /locus_tag="VIW27_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY5738751.1"
                     /translation="MKKTIPALAAFLVFSVFATTTMAADTILRISLQLPLKSHLGQNL
                     LMFKNEVEKKSNGGIEVQIYDSAQLYKDKEVPQAVGSGAIEMGVASLTRFVGDVPAVD
                     VFYLPFMFNTEALVRRAVAPDSPVRSLLDEAILDTGSRVLWWQAYGGAIMLSKGAPVK
                     SPADMVGRKVRVFGKTLGAFTEAAGGSPTLISGSEQYLAYQRGTVDYGMTGVSGVKSR
                     KLWEVMDHITVTNHADIEFIVLVNEKFWQGLSEADRKLISSAARNAEIAVRDEMAKIE
                     ADAYAAAKQNGMTVYTPNAEELTAWKQTAKPVIDGYKAGAGELGERVLKAAQGL"
     gene            4390..4893
                     /locus_tag="VIW27_03445"
     CDS             4390..4893
                     /locus_tag="VIW27_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013961260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY5738752.1"
                     /translation="MKSFGRLTDWLGDLSAWLFFATGAMLTYEVLARYVFAAPTIWAA
                     EISQMFLIWGMYLALPRTIMRRENISIEVIHELLPAWARRSCDFIALAFMIFFCSIVL
                     WYGWDIAWDSFERGRSTGTMLNIPNWWIEMIIPFGFGLSLLACLAGLLRLAQGADLGK
                     PSDETVE"
     gene            4890..6164
                     /locus_tag="VIW27_03450"
     CDS             4890..6164
                     /locus_tag="VIW27_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY5738753.1"
                     /translation="MTTLLILLALFALLLMRVPVGFALGGLGLCLLMIGGFSPLMAPQ
                     AMLGTVDNFVLLAVPLFLLMSNVLLAGGVGRDLFAAVQAWLGHLPGGLAIATVVSCGI
                     FAAISGSSVATAATIGTVAIPEMSQRGYPRPFVLGLLAAGGTLGILIPPSIPMIIFGF
                     ITEESVISLFLAGIGPGLLLIAFFIGFSMIYAWLSPECQASPRATWRERRDTGLRALP
                     ALALAALIIWGIYWGVFTPTEAAAIGFAAALLITGPILRTLTWAGLKQAVTKSMVTTV
                     TILLIVAGAKVFGKAISLYRIPQDVSMFISANFSEAALFVLVVGLVLVLMGLFLEALS
                     MMLIMVPVLSPSLASLGIDPIWFGVFFVIMIECALITPPVGLNIYVIQAVGNASMQEV
                     AKGVLPFLALMLLTVLLIYLVPDIAMYIPFKL"
     gene            6174..7052
                     /locus_tag="VIW27_03455"
     CDS             6174..7052
                     /locus_tag="VIW27_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY5738754.1"
                     /translation="MTDNSPATRLRRLLDEDTCHVMPCCWDGLSAKMIADAGFPFTFM
                     SGFAVSAARLGAPDTGLISFGEMLDQGRNICAATSIPVIGDGDTGYGNALNVRRTVEM
                     YARAGFAAVMIEDQLAPKRCGHTRGKLVVERDEAFNRIRAAVDAREAGADIMILARTD
                     ARHGHGLDEAIERARGFADLGADILFVEAPQSEEEMQRLTSEAPGIHMANLVEGGATP
                     LLPPEQLHRLGYRFAAYPLTLLSAAMKAIQSALASLSKHEHPAEALLEFAELRQIVGF
                     DDYYEAEQNYADRREP"
BASE COUNT         1216 a         2289 c         2180 g         1373 t
ORIGIN      
        1 aggccggcgg cagcggctgg gacgtgttcg tgccgaccaa ttacacgatc acgacctacg
       61 tcggcgagga cctgatcgaa ccgctggacc tgtcgaagct gtcgaatttc aaggccgacg
      121 ccttcgatcc gcgcttctcc gacgccggca ccgtcgacgg caagctctac gcggtgccga
      181 aaaactgggg caccactggc tacgtcgtcg ataacgcgca caacaagggc gcgcgcatga
      241 ccagctggaa ggacttctgg gaccgcacca agaccgattt ttccggtcgc acgatggtgc
      301 acgattacca gctgacgacc atcggcaacg cgctcaagta cttcggctac tcgttcaact
      361 cggtcgaccc gaaagagctc gccgacgccg agaagctatt gctcgaggtc aagccgcacc
      421 tgttcgcgat caacagcgac taccagccgc cgctgcgcaa cggcgacgcc tggatgtcga
      481 tgtgctggac cggcgacggc aagcagctca acagcgatat tccgacgatg gaatacgtgc
      541 tcggtgtcga gggcggcgag atctggagcg attactacgc ggtaccgcgc ggcgccgagc
      601 atcgcgaagc cggctacgcg ctgatcaact tcatgctcga cccggcgatc aacgcgcgcg
      661 aagtgctggc ccacggctac ccggtggccg acagccgcac caacgcgctg ctgccgaagg
      721 agttgctcga ggatccgatc ctgtatccgg cgcaggagct gctcgacgcg ctcgagttcg
      781 gtgcggcggc aacgctgacc gacccgaacc gcgccgaact gctggcgcgc ttcaagtccg
      841 cctaggcgag gcgcggggcg actgaaatga agagcagctg ggcgcaccgg cttttgctgt
      901 tacccgcatc cctgtggctg ctggtgatgc tggtgctccc gctgctcgtc gttttcgtgt
      961 tcagcttcgg cgagcgcgcg cccgcgggcg gctacatacc cgccttcagt ttcgacaatt
     1021 acctgaacct gccggcgcgc ctgaccgcgt tcaagaacac gatgatcatg gcgccgctgg
     1081 gcacgctggc ggcgctgctg atcgcttacc cgctggccta ctacctcgcg gtcagggcca
     1141 acccgagaat caagaccacg ctgctggtga tgatcatcat cccgttctgg accagtatcc
     1201 tgattcgaat ctacgcgtgg atatacatcc tcggcggccg cggcataccg cagctgctgg
     1261 aatggatcgg catcgagggc gtgcgcctga tcaacacgcc caccgcggtg ctgatcggca
     1321 tcatctacgg ttacctgccg ctgatggtgt tcccgattta cgtcagcctg gaaaaattcg
     1381 acaagcggct gctcgaggcc tccgaggacc tcggcgcctc gccgctcaga acctttcgcg
     1441 acgtgaccct gccgctgtcg cttcccggca tcgccaccgg ctgcatgctg gtgttcatcc
     1501 tgctgatggg cgaattcctg atcccggcgc tgctcggcgg cggcaaggtg ttcttcgtcg
     1561 gcaacgcgct ggtcgatttg ttcctgcagt cgcgcaactg gacctacggc tcggcggtgg
     1621 cgaccgcgct ggtcgtcatc atgctggtta ccgtaaccgt ctacatgaaa ctgatcatgc
     1681 gcggcgccgg ccagcgcgaa gacgtgtcgc tgctctagcc tgcctattgc acgaaggcgg
     1741 caccaactct ggaggaaaac tatcaaaatc aaacccgtgt atagaaacta catgaaaaat
     1801 tttgctttca gtttgtgcct gaacgaaatc aggaccaatc cgcaggatgc ggattggcga
     1861 tgcgcagcga ccccgcaggg gtctgcgcca ggaatggcgc agatcagaaa tctggctgcc
     1921 attctcgaac gatctcccct gatccatagc tatggctatg ggtcggcggg cgatcgtccg
     1981 acctcgtttg ctcggcgcgt ccctgcgcct cgcccctgcg gggccgcgct aaagcgcgtc
     2041 caaaatcgct ccaggcgatt ttgtgccaga tttcccctga gtttcgttcc cttcatgcaa
     2101 tatgcaggct agccatgcgc ctctacgcga tcgtgattta cctgttcctc tacctgccga
     2161 tcggcatcat cgtgctgttc agcttcaacg ccggccggca cgcgagcgac ttccagggcc
     2221 tctcggtgaa gtggtacggc aaggtgctgt cgaacccgtt cgcgatggat gcgctggcga
     2281 cgagcctcac ggtggccttc gtcaccgcgc tgctggcgag cctgttcggc accatggccg
     2341 cgctcggtct gcagcaggtc aggggccgcc tgcgcgccct gatcgatgcg gtaatctaca
     2401 tcgccatcat gatccccggc atcgtcatcg gcatcgccac gctgatcgcg ctggtgaccg
     2461 cgttcgattt cctgaacccg gcgctgcagg cctcctggcc ggcatggctc ggcgacgcgc
     2521 cgcggctccg catgggccga gtgtcgctgg tcgcggcgca cacgatgttc acgatggcgc
     2581 tggtgatcgt catcgtgcgc gcgcgcatcg ccggcatgga ccgcaccctg atcgaagcct
     2641 cggccgacct ctacgcgacg ccgatgggca cctttcggca agtgacgctg ccgcaaattt
     2701 tccccgccgt gctggccggc ttcctgctga gtttcacctt cagcttcgac gacttcatca
     2761 tcgcgttttt cgtcgccggt tcggaaacca cgctgccgat ctacgtgttc tcgtctatcc
     2821 ggcgcggcgt tacccccgag atcaacgcca tcggcacgat ggtgctcgcc ttttcgctca
     2881 ccctgctggc cgcggcgcag gtcatgttac gccggcgcgc ccagccgaat tagcgaaagc
     2941 ggcggaagtg aattacgctg gcagttaact tatcttcgcc tcaagcagac tgctacccag
     3001 tttagcggtc gcaccattcc gccgcggcgg cggcgcgcca tcttcgaacc gatcgacaag
     3061 ccgcgccccg agcaaaggcc cgggcagccg gtctgaaatt acggaatttt gcaattccgg
     3121 tgacagttta cttatcttct gcaattacgg agacagttca cttatcttcg aattaagcaa
     3181 actgtcacca taattccgcc cgccggccga attgaatcgc gataatcgtt atgcaagaat
     3241 tgcccggctg cggtttctgc atgcaggccg cgagaataac tagatcacaa caacaacaca
     3301 ggacatcaga catcatgaaa aaaacgattc cggcattggc cgcgttcctg gtattttccg
     3361 ttttcgccac caccacgatg gcggccgata ccatcctcag aatttcgctg cagttaccgc
     3421 tgaagagcca cctgggccag aacctcctca tgttcaagaa cgaggtggaa aagaaatcca
     3481 acggcggtat cgaggtacag atatacgact cggctcagct ctacaaggac aaggaagtgc
     3541 cgcaggccgt cggctccggc gcgatcgaaa tgggtgtggc ctcgctgacg cgcttcgtcg
     3601 gcgacgtgcc ggcggtggac gtcttctacc tgccgttcat gttcaatacc gaagccctgg
     3661 tgcgcagggc ggtagcgccg gacagcccgg tgcgcagcct gctcgacgaa gccattctcg
     3721 acaccggctc gcgcgtgctc tggtggcagg catacggcgg cgcgatcatg ctgtcgaagg
     3781 gcgcaccggt gaaatccccg gctgacatgg tcggcaggaa agtccgcgtg ttcggcaaga
     3841 cgctgggcgc ctttaccgag gctgccggcg gctcgccgac cctgatctcg ggctcggagc
     3901 aatacctcgc ctaccagcgc ggcaccgtgg attacggcat gaccggcgtc tccggcgtca
     3961 agtcgcgcaa gctctgggaa gtcatggacc acatcacggt gaccaaccac gccgatatcg
     4021 agttcatcgt gctggtcaac gagaagttct ggcaggggct gtccgaagca gaccgcaagc
     4081 tgatctcgag cgcggcgcgc aatgccgaaa tcgccgtgcg cgacgagatg gcgaagatcg
     4141 aagccgatgc ctacgcggcg gccaaacaga acggcatgac cgtctacacg ccgaacgcgg
     4201 aagaactgac tgcctggaag cagaccgcaa agccggtcat cgacggctac aaggccgggg
     4261 ccggggaact cggcgagcgc gtgttgaagg ccgcgcaagg cctgtagcga ctccattcaa
     4321 aggctggact cggggtcgtc caccacgggc gaccccgcat cttcacgtgc catttccggg
     4381 ggccatagct tgaaatcatt cggccgactg accgactggc tgggggatct ttccgcctgg
     4441 ctgttcttcg ccaccggcgc gatgctgacc tacgaggtgc tcgcgcgcta cgtattcgcc
     4501 gcgccgacca tctgggccgc cgagatttcg cagatgttcc tcatctgggg catgtacctg
     4561 gcgctgccgc gcaccatcat gcgccgcgaa aatatcagca tcgaggtgat tcacgaactg
     4621 ctgccggcct gggcgcggcg atcctgcgat ttcatcgccc tggctttcat gatcttcttc
     4681 tgcagtatcg tgctctggta cggctgggac atcgcctggg acagtttcga gcgcggccgc
     4741 tccaccggca ccatgctgaa tattcccaac tggtggatcg agatgatcat cccgttcggc
     4801 ttcgggctat cgctgctggc ctgcctggcc ggcctgctgc gcctcgcgca gggcgccgat
     4861 ctcggcaagc cgtccgacga gacagtcgaa tgacgacgct gctgatcttg ctggcgctgt
     4921 tcgcgctgtt gctgatgcgc gtgccggtcg gcttcgcgct cggcgggctc ggcctgtgcc
     4981 tgctgatgat cggcgggttt tcgccgttga tggcgccgca ggcgatgctc ggcaccgtcg
     5041 ataatttcgt gctgctggcg gtgccgctgt tcctgctgat gtcgaacgtg ctgctcgccg
     5101 gcggcgtcgg gcgcgacctg ttcgccgcgg tgcaggcctg gctcgggcac ctgccgggcg
     5161 ggctggcgat cgcgaccgtg gtctcctgcg gcatcttcgc ggcgatctcc ggctcctcgg
     5221 tggccaccgc ggcgaccatc ggcaccgtgg cgattcccga aatgagccag cgcggctatc
     5281 cgcggccctt cgtgctcggc ctgctggccg cgggcggcac gctcggcatc ctgatcccgc
     5341 cgtcgatccc gatgatcatc ttcggcttca tcaccgagga atcggtgatc agcctgtttc
     5401 tcgccggcat cggcccgggg ctgttgctga tcgccttttt catcggtttt tcgatgatct
     5461 acgcgtggct ctcgcccgag tgccaggcgt cgccgcgcgc gacctggcgc gaacgccgcg
     5521 ataccggcct gcgcgcgctg ccggcgctgg cgctggcggc attgatcatc tggggcatct
     5581 actggggcgt gttcacgccg accgaggcgg cggcgatcgg ttttgccgcg gcgctgctga
     5641 tcaccggccc gatactgcgc acgctgacct gggccgggct caagcaggcg gtgaccaaat
     5701 cgatggtaac caccgtcacc atcctgctga tcgtcgccgg cgccaaggtc ttcggcaagg
     5761 ccatctcgct gtaccgcatt ccgcaggacg tatcgatgtt catctccgcg aatttttccg
     5821 aggcggcgct gttcgtgctc gtggtcgggc tggtgctggt gttgatgggc ctgtttctcg
     5881 aagcgctgtc gatgatgctg atcatggtgc cggtgctgtc gccgtcgctg gcgagtctcg
     5941 gcatcgatcc gatctggttc ggcgtgttct tcgtcatcat gatagagtgc gctctgatca
     6001 cgccgccggt gggcttgaat atctacgtga tacaggcggt cggcaacgcc tcgatgcagg
     6061 aggtcgcgaa aggcgtcctg ccgttcctcg cgctgatgct gctgaccgtg ctgttgattt
     6121 acctggtgcc cgatatcgcc atgtacatac cgttcaaact ctaggctcac aagatgaccg
     6181 ataattcgcc cgccacccgg ctgcgccgac tgctcgatga ggatacctgc cacgtcatgc
     6241 cctgctgctg ggacggcctc tccgcaaaaa tgatcgccga cgccggcttt ccgttcacct
     6301 tcatgtccgg cttcgccgtc tcggccgcgc gcctcggtgc gcccgatacc ggcctgatct
     6361 cgttcggcga aatgctcgac cagggacgca atatctgcgc ggccacctcg atcccggtga
     6421 tcggcgacgg cgataccggc tacggcaacg cgctcaacgt gcggcgcacg gtggagatgt
     6481 atgcgcgcgc cggattcgct gcggtgatga tcgaggacca gctggcgccg aagcgctgcg
     6541 gacacacgcg cggcaaactg gtggtcgagc gcgacgaggc gttcaaccgc atccgcgcgg
     6601 cggtagacgc gcgcgaggcc ggcgccgaca tcatgatcct ggcgcgcacc gacgcgcggc
     6661 acgggcacgg tctcgatgag gcgatcgaaa gagcgcgcgg cttcgccgat ctcggcgccg
     6721 atatcctgtt cgtcgaagcg ccgcaaagcg aggaagaaat gcagcggctg accagcgagg
     6781 cgccggggat ccacatggcc aacctggtcg aaggcggcgc cacgccgctg ttgccgcccg
     6841 aacagctgca tcgcctcggc taccgctttg ccgcctaccc gctgaccctg ctgtcggcgg
     6901 cgatgaaggc gatccagagc gcgctcgcat cgctgtcgaa acacgagcac ccggccgagg
     6961 cgctgctgga attcgcggag ctgcgccaga tcgtcggctt cgatgactat tacgaagcgg
     7021 agcagaacta cgcggatcgg cgcgagccgt aatttcga
//
LOCUS       DATCZV010000157         2978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_541045, whole genome shotgun sequence.
ACCESSION   DATCZV010000157 DATCZV010000000
VERSION     DATCZV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541045"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VIW27_03460"
     CDS             <1..405
                     /locus_tag="VIW27_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5738755.1"
                     /translation="YGGKQLHENFAVEISYQDLGRLRKDRGSFDAIAESEGFNLSLLG
                     IIPLGEVAFAYGKAGYMLSDTRYSRVEGDVTTKTDDQRSDFTFGAGFGFRFNHKYDLR
                     AEFESLNDLTDDYVPGGDTITSFSIGGTIYLD"
     gene            484..942
                     /locus_tag="VIW27_03465"
     CDS             484..942
                     /locus_tag="VIW27_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY5738756.1"
                     /translation="MTKILTGLIIALLVAAGAQAAGEGMIKLKSAHSVAVTADRLENV
                     LKSSGMTVLKRIDHAAGAASVGRQLRPTELLIFGNPRVGTPLMQCSPSIAIDLPQKAL
                     IWQDRRGQVWLGYNDPKFLALRHDISGCDELLEQVANALAGFAAAAVAAE"
     gene            1507..2094
                     /locus_tag="VIW27_03470"
     CDS             1507..2094
                     /locus_tag="VIW27_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5738757.1"
                     /translation="MNFLQQMLKPPNLVSALRILFAPLLFALALLQLETWFLAVLLFS
                     AFTDIVDGQLARRLKMTTPLGAHLDSWGDFTIYTTMAVCAWILWPEITRRELVYYAMI
                     LFSFLLPALVGLIKFGKLTGYHTWAVKLAVFVTFVGYVSLYGGFASWPFVLASILCVI
                     AGSEEILITLTLREERTDVRSLLAALKIRRGDEPG"
     gene            2311..2523
                     /locus_tag="VIW27_03475"
     CDS             2311..2523
                     /locus_tag="VIW27_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738758.1"
                     /translation="MNIPTLEEIASTDKLRASLGLAIAEARMARSLLKRRNPAADLER
                     EKQLAELEREIDQLETTLLELKSKDA"
     gene            2839..>2978
                     /locus_tag="VIW27_03480"
     CDS             2839..>2978
                     /locus_tag="VIW27_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005906919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5738759.1"
                     /translation="MKKPTKIVIPVAGLGTRVLPASKVIPKEMLTVVDKPVIQYVIEE
                     AVA"
BASE COUNT          621 a          926 c          862 g          569 t
ORIGIN      
        1 tacggcggca agcagttgca cgaaaatttc gctgtcgaga tctcgtacca ggatctcggg
       61 cgactgcgca aggaccgcgg cagcttcgat gcgatcgccg agtccgaggg tttcaacctg
      121 tcgctgctcg gcatcattcc gctcggtgaa gtggcattcg cctacggcaa ggccggttac
      181 atgttgtcgg atactcgcta ttcgcgggtc gagggtgacg taaccaccaa aaccgatgac
      241 cagcgcagcg atttcacctt cggcgccggt ttcggctttc gcttcaacca caagtacgac
      301 ctgcgcgccg aattcgagag cctcaacgac ctgaccgatg attacgtgcc cggcggcgac
      361 accatcacgt cgttctcgat cggcggcacg atttatctcg actaggccgc gcggcgagtt
      421 gcctgccgaa ggcatacccg cataatgtta acgggaccac taccaaacag gggaggactc
      481 gcgatgacaa aaatcctcac cggcctgata atcgcgctgc tggttgccgc cggcgcacag
      541 gccgccggcg aaggcatgat caagctgaag agtgcgcact cggtagcggt aaccgccgat
      601 cgtttggaaa acgtgctgaa aagcagcggc atgacggtgc tgaaacgcat cgatcatgcc
      661 gcgggcgcgg ccagcgtcgg caggcaattg cggcccaccg agctgctgat cttcggtaat
      721 cccagggtcg gcacaccgct gatgcagtgc agccccagca tcgccatcga cctgccgcag
      781 aaagcgctga tctggcaaga caggcgcggc caggtctggc tcggctacaa cgatccgaaa
      841 tttctcgctt tacgtcatga catcagcggc tgcgatgagc ttctcgagca ggtcgccaat
      901 gcgctcgccg gcttcgccgc cgccgccgtg gccgccgaat gacccatgcg caccggcgag
      961 ccggcgtagg ctagcgcatc gtggcgtatt gaagtggatg ccgcggtccg gcgttccggc
     1021 tcgaaggccc actgctgtcc ggaataagct acgacctgct gctctctcgg caaacgacaa
     1081 tctgccatcc cggccctggg ctgaggcctc ttaccgagca ggtttcgaat cttgaccggt
     1141 tgttgattca accaaggccg gcctactggc gatgtttaag gcttcgctgg caattgcgaa
     1201 agatgccggg tcaagcccgg catgacgtct tcggcataat gctcagacca aaatcgagtc
     1261 gcaatcgtga attaatcatg taatgcctga aaactgtacc aatagccgtg ccgaagccgg
     1321 caaccgacag ccagccgcgc gacggcctgc ctgaatatgc ccggttaccg aacagcggct
     1381 tgtagcgcat aatacgcctt tgacggcaaa ccgagagcca gtgatgatat gtgacgctgc
     1441 ggcgtattgc aggcacgcca cgcaccgccg agcgcggcgc ggtcgacccg atgcaggcgg
     1501 cgggcaatga actttctcca gcaaatgctc aagccgccga acctggtcag tgcgctgcgc
     1561 atactgttcg caccgctgct gttcgcgctc gcgctgctgc agctcgaaac ctggttcctc
     1621 gcggtgctgc tgttctcggc gttcaccgac atcgtcgacg gccagctggc gcggcgcctg
     1681 aaaatgacca ccccgctcgg cgcccatctc gacagctggg gggatttcac catctacacg
     1741 acgatggccg tctgcgcatg gatcctgtgg ccggagatca cgcgccgcga actggtttac
     1801 tacgcgatga tactgttcag ctttttgctg ccggcgctgg tcgggctgat caagttcggc
     1861 aagctcaccg gctaccacac ctgggccgtg aagctggcgg tattcgtcac cttcgtcggc
     1921 tacgtctctc tttacggcgg ctttgcgagc tggccgttcg tgctcgccag tatcctctgc
     1981 gtcatcgccg gcagcgagga aatcctgatc acgctgacgc tgcgcgagga gcgaaccgac
     2041 gtgcgctcgc tgctggctgc gctgaagatt cgcagaggcg acgaaccggg ctagccggcg
     2101 gcgctgccgc ggccatccct taacctataa aatgcattaa ataataacta taaatgtttg
     2161 tttttattgt ttatttttat aatccagaat gctcgcttcg ggtcgctatc gacaggcgta
     2221 agattaatcc tacaaaaatt cgccgggatt gcgatttccc gcgccgcaca atcctgcgat
     2281 atttgccgtg atggtgattc ggggacacaa atgaacatac cgacactcga ggaaattgcc
     2341 tccaccgata agctgcgcgc cagcctcgga ctcgcgattg ccgaagcgcg catggccagg
     2401 tctctgctga aacggcgcaa cccggcagcc gatctcgagc gggaaaagca actcgccgag
     2461 ctcgaacgcg agatcgacca actcgagacc acgctgctcg agctgaagtc gaaggacgct
     2521 taataaatca gggccggcgc gcgatttcct ggcccctgct cccggccggc cgactgaacc
     2581 ggcaggtagg cacccgtaaa cccgctcgac ctcctccgag gcttcgcccg acggccgcgg
     2641 ccgaaggcaa tcgatgcagg gacgcgagcc ctcgcgccga tccgcaactc tatgttataa
     2701 aaggcgcggc gataacgcgc ccgcgcgcct gcccgatcgg tcgcgggaca aacattcgta
     2761 ctcgacccgt cgttctgcat tacaattagc tgaaacaaca cagttcccgc aatcgacttt
     2821 aagacaactg ttctacccat gaaaaaaccg acaaaaatcg tgattccggt ggccggcctg
     2881 ggtacccggg tgctgcccgc cagcaaggtg ataccgaaag aaatgctgac cgtggtcgac
     2941 aagccggtga tccagtacgt gatcgaggaa gcggtcgc
//
LOCUS       DATCZV010000158         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_170223, whole genome shotgun sequence.
ACCESSION   DATCZV010000158 DATCZV010000000
VERSION     DATCZV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170223"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VIW27_03485"
     CDS             complement(<1..258)
                     /locus_tag="VIW27_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5738760.1"
                     /translation="MNCRIAKSLMQDAADGKLNGADTGLLNAHLEECATCRKEYRQTA
                     MLLEALRAIPVPPPSPGFAERALRNATRSQPPATRSHHFRLA"
     gene            complement(255..839)
                     /locus_tag="VIW27_03490"
     CDS             complement(255..839)
                     /locus_tag="VIW27_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5738761.1"
                     /translation="MSIINLFRNREKSADRFECLLRPHIDPMYRFAYRLCGASDDAEE
                     LVQVFLTRLFPKLERIEKIDKLLPWLCRGLYNLYVDGYRRQQREITIFDTDEQLYEGS
                     DDTDTTFIGASSAELSSQIESALEQLNPDQRLVVLLHDSEGYTLEELSQILRVPLGTL
                     KSRLNRAHKVLTKLLQTEPFGDPVRVRDIERRNS"
     gene            1427..1723
                     /locus_tag="VIW27_03495"
     CDS             1427..1723
                     /locus_tag="VIW27_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738762.1"
                     /translation="MGSKRVAAARVTGLSNITCTEVQVKPILLDGSWRNRECVSALLE
                     VDVAKAGPAFANHVVVSARSAVDPRRRHHRQLDAFVEGNRVAEQQAEAGSLQGS"
     gene            complement(1720..>2733)
                     /locus_tag="VIW27_03500"
     CDS             complement(1720..>2733)
                     /locus_tag="VIW27_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738763.1"
                     /translation="EIEGEQDLTGPGSYPVTLSLEPREEKAAPIVSEQAPAATGGDDA
                     DSDEPPAAQIRIVPVPDSNSLLIFATPSQFASIELALEELDTPPLQVLMEVTIMDVQL
                     TGDLAYGVQWFIENGSPGDSDSITIGDALTFPQTLSYAAVRDGGDLRAIFGLLSADGK
                     VNVLSSPSVLVRNNQSASIRIGNQQPIGTAALNPDGTIIASSVTYRDTGVLLEIEPSI
                     TSSGTVNVQLSQQVTDVGEIDDATGQRTFLNRNLSTSVSVSNGETIILGGLIRTNKAI
                     TKSGVPGLRDIPGLGLLFGKTITSDVRVELLMMLSPRIIRSPAEHNTVIEEYKAKFQS
                     LEF"
BASE COUNT          458 a          798 c          932 g          545 t
ORIGIN      
        1 ggccaggcga aagtggtgac tgcgggttgc cggcggctgc gagcgggtcg cattgcgcag
       61 tgctcgctcg gcaaagccgg gcgacggcgg cggtaccgga atagcccgca gggcttcgag
      121 cagcatggcc gtttgccggt actctttacg gcaggtggca cactcttcga ggtgcgcatt
      181 gagcaggccg gtatcggcgc cgttgagctt gccgtccgcg gcgtcctgca tcagtgattt
      241 tgcaatacgg cagttcacga atttctcctc tcaatatccc ttacacgaac gggatcgccg
      301 aaaggttccg tctgtagcaa tttagttagc accttgtggg cgcggttgag gcgcgatttc
      361 agcgtgccca gtggaacccg caggatctgc gacagctctt cgagggtata cccctcggag
      421 tcgtgcagca ggaccaccag tcgctgatcg gggttgagtt gctccagcgc cgactcgatt
      481 tgcgaggaca gttcggcgct gctggcgccg atgaaggtgg tgtcggtatc gtccgatccc
      541 tcgtagagtt gctcgtcggt atcgaaaatg gtgatttcgc gctgctgccg gcgatagccg
      601 tcgacgtaga ggttatagag gccgcgacac agccacggca gcagcttgtc gatcttctcg
      661 atccgctcca gcttcgggaa caggcgcgtg agaaagacct gcaccaactc ctcggcgtcg
      721 tcggacgcgc cgcacaggcg gtaggcgaag cggtacatcg ggtcgatatg cgggcgcagc
      781 aggcattcga agcgatcggc tgacttttcc ctgttcctga agaggttgat aatgctcacg
      841 aaaatctcgg tcccgtctgc tcaaatcgcg tcccaaaccg gcgttcgcca agccccggga
      901 aaaccgcggc agggcagggt acgggccgcc cggagcccgg tgatttaggc ctccgcgaca
      961 gtctctcgcc ggaagttcga atctaccaga ttccggctta aaagccttgt attcgtgggg
     1021 ttttggcggc cgctaatgtg cctaattgtg acgggcttcg caatcgcgtg gcgaaaacga
     1081 cgccgaccct gcaaacgggg ctgtcagggg tcgtcgacga ggtcggcgtc agcatcaatg
     1141 gcgtcgcggg gaaagggcac ggaatggccg ccggcgcccg gaatggccgc cggcgcccgg
     1201 aatgctgggg ccctggcggt tcggtcggcg ggcaaaccgc tggaccacgc ggccaccgtg
     1261 gtggtcgatc tgtcagcggc cgggcaagca gtggtcgctg gtttatcggc ccgcatgctg
     1321 gcggtaataa tcgtgaaaca tccccgcttc gattgcgctg cagcgccgcg aggcgctgcg
     1381 cgagtcgacg gaattacggt gattgcctgg cgcggatcag tcaatcatgg gaagcaagcg
     1441 cgtagccgcc gcccgcgtga ctggattgtc gaacattacc tgtaccgagg tacaggttaa
     1501 acccatactt ttggatggtt cgtggagaaa tcgcgaatgc gtttcggccc ttttagaagt
     1561 cgatgtggcg aaagccgggc ctgcattcgc caatcacgtc gttgtgtcgg ctcgatccgc
     1621 ggttgatccg cgccgacggc atcatcgcca gctcgacgcg ttcgtcgaag gtaatcgtgt
     1681 tgccgaacag caggccgagg ccgggagtct tcagggcagc tagaattcga ggctctggaa
     1741 cttggccttg tattcttcga tgacggtgtt gtgctcggcc gggctgcgaa tgatacgcgg
     1801 cgacagcatc atcagaagct cgacgcggac gtcggaggta atggtcttgc cgaacaggag
     1861 gccgagcccc ggaatatccc gcagccccgg cacgccgctc ttggtgatcg ccttgttggt
     1921 gcgaatcagg ccgccgagaa tgatggtttc gccgttgctg accgataccg acgtgctcag
     1981 gttgcggttg agaaaggtgc gctgcccggt ggcgtcgtcg atctcgccga cgtcggtgac
     2041 ctgctgcgac agctgcacgt tgaccgtgcc gctggaggtg atcgacggct cgatctccag
     2101 cagcacgccg gtatcacggt aggtcaccga gctggcgatg atggtgccgt cggggttcag
     2161 cgccgcggtg ccgatcggct gctggttgcc gatacggatg gacgcgctct ggttgttgcg
     2221 caccagcacc gacggtgacg acagcacgtt gaccttgccg tcggcggaca gcaggccgaa
     2281 gatcgcgcgc aggtcgccgc cgtcgcgtac cgcggcgtag ctcagggttt gcggaaaggt
     2341 cagtgcgtcg ccgatggtaa tcgagtcgct gtcgccgggg ctgccgtttt cgatgaacca
     2401 ctgcacgccg taggccaggt cgccggtcag ctgcacgtcc atgatggtca cttccatcag
     2461 cacctgcagc ggcggcgtgt cgagttcctc gagggcgagc tcgatgctgg cgaactggct
     2521 gggcgtggcg aagatcagca gcgagttgct gtcgggtacc ggcacgatgc ggatctgcgc
     2581 cgccggcggc tcgtcgctgt cggcgtcgtc gccgccggtg gctgccggcg cttgttcgct
     2641 gacgatcggc gcggcttttt cttcccgggg ctcgaggctc agcgtcaccg ggtaggatcc
     2701 cggcccggtg aggtcttgct cgccctcgat ctc
//
LOCUS       DATCZV010000159         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_593532, whole genome shotgun sequence.
ACCESSION   DATCZV010000159 DATCZV010000000
VERSION     DATCZV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593532"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VIW27_03505"
     CDS             complement(<1..504)
                     /locus_tag="VIW27_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738764.1"
                     /translation="MNLRDLKYIVAVADSGHFGRAARHCFVSQPTLSGQIKKLEAELG
                     VMIFERTNRRVAVTPTGEEILRHARLMIEQSDAITQIARARQDPLAGPLRIGAIPTLS
                     PYLMPLILLPLKKRHPQMKLVLSEETTETLLQRLAHHEIDAALLATAEDESDLDRIEL
                     FDEPFWIA"
     gene            complement(625..1554)
                     /gene="ppk2"
                     /locus_tag="VIW27_03510"
     CDS             complement(625..1554)
                     /gene="ppk2"
                     /locus_tag="VIW27_03510"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY5738765.1"
                     /translation="MDKQTELDADEPATDEDSSLAEPEDNRMNIIRRFENGMYPYPRK
                     IGRVAYEKEKASLQVELLKVQKWVKSTGKKIVIIFEGRDAAGKGGAIKRFMEHLNPRG
                     ARVVALEKPTIAEQTQWYFQRYINHLPSAGEIVLFDRSWYNRAGVERVMGFCDPAAYL
                     EFMRQCPEIERMLVRSGVLLFKYWFSVTQDEQRRRFDSRKSDPLKQWKLSPVDQASID
                     KWDDYTEAKEAMFFYTDTADAPWTIVKSDDKKRARLNCMRHFLSTLDYDDKDEETVGK
                     PDPFIVTSSDHAIGNSEHILGKSLHPGLRRSRK"
     gene            1770..2243
                     /locus_tag="VIW27_03515"
     CDS             1770..2243
                     /locus_tag="VIW27_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY5738766.1"
                     /translation="MAQLVEQLAAALLREGMRACTAESCTGGLIAKTFTDLAGSSDWF
                     ERGFVTYSNEAKSEMLGVPAALIADYGAVSEPVAVAMASGALQHSHAGLAIAVTGIAG
                     PAGGSDDKPVGTVWIAVATADEQRAARYFFDGDRRAVRTASARQAIADARRLLGD"
     gene            complement(2262..3449)
                     /locus_tag="VIW27_03520"
     CDS             complement(2262..3449)
                     /locus_tag="VIW27_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019616041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5738767.1"
                     /translation="MEALHFSVSSQFLTTLGVILLAGLLISALGQRTLLPRATLLILF
                     GMIIGGEMLDLLPRLVSAQFPLVADLTLLMVGFLLGGKLTRSSLQEHSSLSLGISLCA
                     ALLPAALVCAGMLAFGIQLEIAILLGCFAAATAPAAVFDVVEESGIRNRFSELLLLVV
                     VLDDVWGLMLFAIGMAVVSSLNGAAGDVGAATLIVHELGGAILLGAVIGLPAAYLTGR
                     IRPGKPVMSEAIGIVLLCGGLAAWLQVSYLIAAITMGAVITNLARHHEHPFHAIEGIE
                     SMFMLVFFVLAGASLEIGALPTVGLIALVYIICRSAGKLAGGWLGNRVGGAVAGRLDW
                     IGAALLPQAGVAIGMALVASIHFPGYHQIMLPIVIASTIIFEIAGPVFTRHALRHSLR
                     QAD"
BASE COUNT          612 a         1093 c         1135 g          616 t
ORIGIN      
        1 ggcgatccag aagggttcgt caaacagctc gatgcgatcg aggtcggact cgtcctcggc
       61 ggttgccagc agcgcggcgt cgatttcgtg gtgcgccagt cgctgcagca gggtttcggt
      121 ggtttcctcc gacagcacca gtttcatctg cgggtggcgc tttttgagcg gcagcaggat
      181 caacggcatc aggtaggggc tcagggtcgg aatcgcgccg atcctgaggg ggccggccag
      241 tgggtcctgg cgggcgcggg cgatctgcgt gatcgcgtcg gactgttcga tcatcaggcg
      301 cgcatggcgc aggatttcct ccccggtcgg cgtcaccgcg acacggcgat tggtgcgttc
      361 gaaaatcatc acgccgagtt cggcttcgag tttcttgatc tgcccgctca aggtaggctg
      421 actgacgaaa cagtgccgcg cggcgcggcc gaagtggccg ctatcggcga ccgcgacaat
      481 gtatttgagg tcgcgcaggt tcatggctct tcaataggca atgacgatca ttgaacctta
      541 aacgaaaggc gtggcaagca ccatgttttc gtcatcaccg cttatcgggt cagccccggc
      601 agcgattgcg cgcggtgcgc aggcctactt gcgcgagcgc cgcaggcccg ggtgcaggct
      661 cttgccgagg atatgctcgc tgttgccgat cgcatggtca ctggaggtga cgatgaacgg
      721 atcgggctta ccgacggttt cctcgtcctt gtcgtcgtaa tccagcgtcg acaggaaatg
      781 ccgcatgcaa ttgagtcgcg cgcgtttctt gtcgtcggac ttgacgatgg tccagggggc
      841 gtccgcggta tcggtgtaaa agaacatggc ctccttggcc tcggtgtaat cgtcccactt
      901 gtcgatcgag gcctggtcga ccggcgacag tttccattgc ttgagcgggt cgctcttgcg
      961 cgagtcgaag cgccggcgct gctcgtcctg ggtgaccgag aaccagtatt tgaacagcag
     1021 cacgcccgag cgcaccagca tgcgctcgat ctccgggcac tggcgcatga actccagata
     1081 ggcggcgggg tcgcaaaagc ccatcactcg ctcgactccg gcacggttgt accaggagcg
     1141 gtcgaagagc acgatttctc cggccgaggg cagatggttg atatagcgct ggaagtacca
     1201 ctgggtctgt tcggcgatgg tgggcttttc gagtgccacg acgcgcgcgc cgcgcgggtt
     1261 caggtgctcc atgaagcgct tgatcgcgcc acccttgccg gccgcgtcgc ggccctcgaa
     1321 gatgatgacg atcttcttgc cggtcgactt gacccacttc tgcaccttca gcaactcgac
     1381 ctgcaagctc gccttttcct tttcgtaggc gacccgtccg atcttgcgtg gataggggta
     1441 catgccgttt tcgaagcgac gaatgatatt catgcgattg tcttccggct ccgcgaggct
     1501 gctgtcctcg tcggttgccg gctcgtcggc gtcgagttcg gtctgtttgt ccatggtgtg
     1561 cgtggttttc ggaaacgggc cccgtatact aatgcaatcc gccaccccga tgaagcccgc
     1621 gcgccggatt tccggcctgc gcggtcgggc gcggcgcggc gtttgccttt cgccgccgcc
     1681 agcggcgatg cgcgatctga cgccgcaagg cgtgaatcga gccgcgatga aactgccttt
     1741 ttggctataa tggcgcgatg tcttcgaaga tggctcaact ggtcgagcaa ctggccgcgg
     1801 cgctgctgcg cgagggcatg cgcgcctgca ccgcggaatc ctgcaccggc ggcctgatcg
     1861 ccaagacctt taccgacctg gccggcagtt ccgactggtt cgagcgtggt ttcgtcactt
     1921 acagcaacga ggccaagagc gaaatgctcg gcgtgccggc tgcgctgatc gccgattacg
     1981 gtgcggtcag cgaaccggtt gccgtggcca tggcaagcgg tgcgctgcag cacagccacg
     2041 ccggtttggc gatagcggtc accggtatcg ccggcccggc cggcggcagc gacgacaaac
     2101 cggtcggcac ggtgtggatc gcggtggcca cggccgacga gcagcgcgcc gcgcgttatt
     2161 ttttcgacgg cgaccgccgt gccgtgcgta ccgccagcgc gcggcaggcg attgccgatg
     2221 cccgccggct gctcggcgat tgatgccggc cgctgcgggc gctagtccgc ctggcgcaga
     2281 ctgtgccgca gggcgtgacg agtgaacacc ggccccgcga tctcgaagat tatcgtcgag
     2341 gcaatgacga tcggcagcat gatctggtgg tagccgggaa agtggatcga tgcgaccagc
     2401 gccatgccga tcgcgacacc ggcctgcggc agcagcgccg cgccgatcca gtcgagccgc
     2461 ccggcgaccg cgccgccgac ccggttgccg agccacccgc cggccagctt gccggccgag
     2521 cggcagatga tgtagacgag cgcgatcagg ccgactgtcg gcagcgcgcc gatctccagc
     2581 gaggcgccgg cgagcacgaa aaacaccagc atgaacatcg attcgatgcc ctcgatggca
     2641 tggaacgggt gttcgtggtg gcgcgcgagg ttggtgatca ccgcgcccat cgtaatcgcc
     2701 gcgatcaggt aggaaacttg cagccacgcc gccagtcccc cgcagagcag cacgataccg
     2761 atcgcttcgc tcatcaccgg cttacctggc cgaatgcggc cggtcaggta agcggcaggc
     2821 agcccgatga ccgcgccgag caggatcgcg ccgccgagtt cgtgaacgat gagggttgcc
     2881 gccccgacat cgccggccgc cccgttcagc gaggaaacca ccgccatacc gatggcgaac
     2941 agcatcagtc cccagacgtc gtcgagcacc accaccagca gcagtagttc gctgaaccgg
     3001 ttgcgaatgc cggattcctc gaccacgtcg aaaaccgccg ccggtgccgt cgcggccgca
     3061 aagcagccga gcaatatcgc gatctccagt tggatcccga acgcgagcat gccggcgcaa
     3121 accaacgccg cgggcagcag cgcggcgcac agtgaaatcc ccagcgacag gctgctgtgt
     3181 tcctgcagcg aactgcgcgt cagtttgccg ccgagcagga aacccaccat cagcagtgtc
     3241 aggtcggcca ccagcgggaa ctgcgcgctg accagtcgcg gcagcagatc gagcatttcg
     3301 ccgccgataa tcatgccgaa caggatcagc agcgtggccc gcggcagcag cgtgcgctga
     3361 ccgagggccg agatcagcaa cccggcaagc aggataaccc cgagcgtggt tagaaactgg
     3421 gacgaaacgc tgaaatgcag ggcctccatc ggcacc
//
LOCUS       DATCZV010000160         3548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_11419, whole genome shotgun sequence.
ACCESSION   DATCZV010000160 DATCZV010000000
VERSION     DATCZV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11419"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VIW27_03525"
     CDS             <1..1044
                     /locus_tag="VIW27_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5738768.1"
                     /translation="DPNIVAKLERGEEQRIRPCVGVTHCIGNHRPTCVHNAASGRELQ
                     WPQRIERAAGMARRAVVVGAGPAGLEAGRILAERGHRVVIFEATDRPGGQMRLAATAS
                     WRRDVVGIVDWRVAELERLGVEIRYNTLAEAADVLVENPDIVIVASGGLPDLDWLPGA
                     ELATPSWDILSGSVKPAAKVVVYDGNGRHTGATAAEYCHDAGSEVQLVMLDEMAVAEL
                     EYGERVIWRREFARRGLVPLTEYALLALTPAAAGIDAHLRHELSGEERVLNAEQVVVE
                     RGTLPFVDVFDSLRDDSANQGVTDIDALLDGRPQPGLEQDDGYALFRIGDAASSRNLA
                     AAMFDALRLCSTL"
     gene            complement(1061..2428)
                     /locus_tag="VIW27_03530"
     CDS             complement(1061..2428)
                     /locus_tag="VIW27_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5738769.1"
                     /translation="MSDSLLSDIRALVGDAGLLTGDAVSGRSAGIWSTAPLEALALVR
                     PATTDEVAGVLRLCHAAGQSVIPVGGMTGLAGAHTSGAGDLLLSTERMKRIESVDTEA
                     RSMVVEAGAVLQQVQQAAEDAGLYFALDLGARASCTIGGNIATNAGGVRVLRYGMMRE
                     QLLGLEVVLADGQVVSSMFNLLKNNTGYDLRQLFVGSEGTLGVITRAVLRLHEAPPEV
                     QTALLAVPGWPQVMSLLRYFDASAPGQLAACEVMWANDYELNTGEFSAIDPPLQPGAP
                     YYLLIDLYAAEREEFENRLADSFERGDVVDGVIAQSGAERERLWRIREDFEPEQRRFG
                     DIIGYDVSLPIQQMESMVETVSRELKARDAAAELFAYGHVGDGNLHYTIYPASLPHAE
                     VDELVYRPLAAVRGSISAEHGIGIDKKAYLHYTRNDAEIALMRQLKRALDPANLLNPG
                     KLFDL"
     gene            2664..>3548
                     /locus_tag="VIW27_03535"
     CDS             2664..>3548
                     /locus_tag="VIW27_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY5738770.1"
                     /translation="MIEIIEALSLPALFTVVLLAAVVIGIAGTRIVFVVESISHKSGL
                     GDAISGALFIGIATSLSGSVLSISAASEGHTGLAISNAMGGIVVQTFFLVIGDLLYRR
                     ANIEHAAASLENTMQATLSILLMAVVLFFHFGPELAFFGIHPGSFLLVAIYITGLLVI
                     RQSHQFPQWIARRSAETRDDETRESMPDETLGKLLIRFTLLALLLVATGLVLTRATLA
                     LSSQTGISENVLGALLTSTLTSLPELVTMITAIRRGALTLAVGNIIGGNTFDVLFLSA
                     SDAAYRDGSLFHTMTGEHL"
BASE COUNT          566 a         1147 c         1169 g          666 t
ORIGIN      
        1 gacccgaata tcgttgccaa gctcgaacgc ggcgaagaac agcgcatacg cccctgcgtc
       61 ggggttacgc actgcatagg caaccatcgt ccgacctgcg tgcacaacgc ggccagcggg
      121 cgtgagctgc agtggccgca gcgcatcgag cgcgctgccg gcatggcgcg gcgggcggta
      181 gtcgtcggcg ccggcccggc cgggctggag gcgggccgga tcctcgccga gcgcggccac
      241 cgggtggtaa tcttcgaggc caccgaccgc cccggcgggc agatgcgcct ggccgccacg
      301 gcgagctggc gacgcgacgt ggtcggcatc gtcgactggc gggtggccga actcgagcgc
      361 cttggcgtcg aaatccgcta caacacgctc gccgaggccg ccgatgtgct ggtcgagaat
      421 cccgatatcg tgatcgtcgc cagcggcggc ctgccggatc tcgactggct gccgggtgcc
      481 gaactcgcca ccccgtcctg ggatatcctg tcgggctcgg tgaagccggc ggcgaaggtg
      541 gttgtctacg atggcaacgg ccgtcacacc ggcgcgaccg cggccgagta ctgccacgac
      601 gccggcagcg aggtacagct ggtgatgctc gatgaaatgg ctgtcgccga actcgagtac
      661 ggcgaacgcg tcatctggcg gcgcgaattc gcgcgccgcg gactggtgcc gttgaccgag
      721 tacgcattgc tggcgctgac gcccgcggcc gccggcatcg atgcccacct gcgccacgag
      781 ctgagcggcg aggaacgcgt gctgaatgcc gagcaggtgg tcgtcgagcg tggcacgctg
      841 ccgttcgtcg atgtctttga cagcctgcgc gacgattccg ccaaccaggg agtgaccgat
      901 atcgacgcgt tgctcgacgg caggccgcag cccgggctcg aacaggacga cggctatgcg
      961 ctgtttcgca tcggcgacgc cgcgtcgagc cgcaacctgg ccgcggcgat gttcgacgcg
     1021 ctgcgcctgt gctcgacgct ctgaagcgcg gaattcggct tcacaaatcg aacagcttgc
     1081 cgggattcag caggttggcg ggatcgaggg cacgcttcag ctgtcgcatc agcgcgatct
     1141 cggcgtcgtt gcgcgtgtag tgcaggtagg ctttcttgtc gataccgatg ccgtgttcgg
     1201 cggaaatcga cccgcgaacg gccgccagcg gccggtagac cagttcgtcg acctcggcat
     1261 gcggcaggct tgcgggatag atcgtgtagt gcaggttgcc gtcgccgacg tgcccgtaag
     1321 caaacagctc cgccgcggcg tcgcgcgcct tgagctcgcg gctgacggtt tcgaccatcg
     1381 actccatctg ctggatcggc aggcttacgt cgtagccgat gatgtcgccg aagcggcgtt
     1441 gttccggttc gaaatcctcg cgaattcgcc acagccgttc gcgttccgca ccggactgcg
     1501 cgatcacacc gtcgacgaca tcaccgcgct cgaaactgtc ggccagccgg ttttcgaact
     1561 cttcgcgctc ggccgcgtac aggtcgatta acaggtaata gggtgcgccg ggctgcagcg
     1621 gcgggtcgat agccgaaaat tcgccggtat tgagttcgta gtcgttggcc cacattacct
     1681 cgcaggcggc aagctggccg ggggccgaag catcgaaata acgcagcagc gacatgacct
     1741 gtggccagcc gggcaccgcg agcagcgcgg tctgtacttc cggtggggcc tcgtgcaggc
     1801 gcagcacggc gcgggtgatc acgccgagcg tgccttccga gccgacgaac agctgtcgca
     1861 ggtcgtagcc ggtattgttt ttcagcaggt tgaacatcga cgacaccacc tggccgtcgg
     1921 cgagcacgac ctcgaggccg agcaattgct cgcgcatcat gccgtagcgc agcacgcgca
     1981 cgccgccggc attggtggcg atattgccgc cgatcgtgca cgaggcccgc gcgccgagat
     2041 cgagcgcgaa gtacagcccg gcgtcttcgg cggcctgctg aacctgctgc aggaccgcgc
     2101 cggcctcgac gaccatgctg cgagcctcgg tatcgaccga ctcgatgcgt ttcatgcgct
     2161 cggtcgacag cagcaggtcc ccggcgccgg aggtgtgcgc accggcgagc cctgtcatgc
     2221 cgccgaccgg gatcacgctc tgcccggccg cgtggcacag ccgcaacacg ccggcgacct
     2281 cgtcggtagt agccggtcgc accagcgcca gcgcctcgag cggcgccgtc gaccagatgc
     2341 cggcgctgcg cccgcttacg gcgtcgccgg tcagcaggcc ggcgtcgccg accagcgcgc
     2401 gtatatcgga taacaggctg tcgctcatga ccccggattt tgaaccgccg accctgctgc
     2461 tgcaagcggc tcgatcgtca cggctggccg gtctcgatcc ctctgcacgc gtcgtcgagg
     2521 gcggcgatga gttcgcttcg agtttctccc cccaatcttt gggccgtcac ttgtcgacgg
     2581 gcctctgcct cttcctgcga gccgttgcag tggtctggca agccttgatt tgagactatg
     2641 gagcgagttt cggactgcgg ttaatgatcg aaatcatcga agcactgtct ctgccggccc
     2701 tgttcacggt cgtgctgttg gccgctgtcg tcatcggcat cgccgggacg cggatcgttt
     2761 tcgtggtcga gagcatttcg cacaaatccg gcctgggcga cgccatttcg ggggcattgt
     2821 tcatcggcat cgcgacttcg ctgtcgggat cggtgctcag catcagcgcg gcgtccgagg
     2881 gccataccgg gctggcgatt tccaatgcga tgggcgggat cgtcgtgcag acgtttttcc
     2941 tggtaatcgg ggatctgctg tatcggcgag cgaatatcga acatgcggcc gcttcgctgg
     3001 aaaacacgat gcaggccacg ctgtcgattc tgctgatggc tgtggtgctg tttttccact
     3061 tcgggccgga gctggcgttt ttcggtattc accccggatc atttctgctg gttgcgatct
     3121 atatcaccgg gttgctggtg atacggcaat cgcaccaatt tccacaatgg attgcccggc
     3181 gatccgcgga gacccgcgat gacgagactc gcgaatcgat gccggacgaa actctcggca
     3241 aattgctcat ccgcttcacc ctgctggctt tgctgctcgt cgcgaccggc ctggtgctca
     3301 cccgggcaac cctggcgttg tcgtctcaga ccggcatatc cgagaatgtt cttggcgcgc
     3361 tactcacctc gaccctgacc tcgctgcccg aactggtgac gatgatcacc gcgattcggc
     3421 ggggtgcgct gacgctggcg gttggtaaca ttatcggcgg caacaccttc gacgtattgt
     3481 tcctctccgc ctccgacgcc gcctatcgtg atggttcgct gtttcatacg atgaccggcg
     3541 agcacttg
//
LOCUS       DATCZV010000161         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_593867, whole genome shotgun sequence.
ACCESSION   DATCZV010000161 DATCZV010000000
VERSION     DATCZV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593867"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1092
                     /locus_tag="VIW27_03540"
     CDS             211..1092
                     /locus_tag="VIW27_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738771.1"
                     /translation="MLNHLRAIYIFVKAVELGSFRATAKALSLSPSVVSYQISRLEKD
                     LEVALLYRSTRRLSLTSEGRELYELMAALVAGAERGVDQLTAALSEPVGRLQVTLAAS
                     FSRENITRQIAAFARQHPRVEMSVRFSDEQKDLIADGIDLAIRSGPIADSSLQARKLF
                     SLERILVASPRYLARMPRAEQPDDLADWDWIWLDVTAEHREFRHRQSGERQNVKLNKR
                     VIVNDGYAMCEFAVEGLGVLTSPDYLVEEYLRQGQLVEVLPDWRLAPIDVYAIWPPNS
                     PRTGLTRRFVDHLLEHR"
     gene            1172..2329
                     /locus_tag="VIW27_03545"
     CDS             1172..2329
                     /locus_tag="VIW27_03545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5738772.1"
                     /translation="MTEDRSYPEVRDAVARLCRDYPDTYWRDKDRDKAYPEEFVQALT
                     DAGYLSVLIPEEYGGAGMDLGAACAVLEEIQRSGANGAACHAQMYIMGTLLRHGSEQQ
                     KQKYLPDIAAGRLRLQAFGVTEPSAGTDTTRIKTRAERDGDDFVVNGQKVFISRVEHS
                     DLLLLLVRTTSRDAVDKPSRGMSVLLVDLRDAIGKGLTVKPLATMLNHATTELFFDNL
                     RVPAANLIGEEGKGFGYILDGMNAERILIAAECIGDARWFIDRAGNYAAQREVFGMPI
                     GKNQGIQFPIARAHVQTEAAALMVDKACRLFDAGEPCGSEANRAKLRAADASWAAADM
                     CLQTHGGYGFTVEYDVERKFRETRLYQVAPISTNLILSNIATHVLGLPKSF"
     gene            2329..>3016
                     /locus_tag="VIW27_03550"
     CDS             2329..>3016
                     /locus_tag="VIW27_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5738773.1"
                     /translation="MLKLDDILVVSLEQAVAAPFCSNRLARAGARVIKIERAEGDFAR
                     GYDDVVNGESAYFVWLNQGKESLVLDFKRAADAELLHRLLASADVFIQNLAPGAAARA
                     GFGSDALRERYPRLICCDITGYGDSGEYRRMKAYDLLVQCESGMASITGTPEAPGRVG
                     VSACDINCGQQAYAAILEALIARESTGSGCVVKVSLFDAMAEWLAVPLLHYDYGGRVQ
                     GRVGLNHATIS"
BASE COUNT          562 a          956 c          950 g          548 t
ORIGIN      
        1 cgaggccgcg catgtggtcg gtgtatttcg acagggactg gcgaaagccc tcgagttcga
       61 acccgggttt catgtcgaaa caggtcagca tgtgaaacat cgttcctccg gagggtcagg
      121 gatacaaaat gtcggcgacc agattaaacc gatggtcgat atagaaacaa gtcagtacaa
      181 ttcagaatca ctattctaat actttaaata atgctgaatc acctgcgtgc gatttacatt
      241 ttcgtcaagg cggtcgaact gggctcgttt cgcgcgaccg cgaaagccct gtcactgtcg
      301 ccgtcggtgg tcagctacca gatttcacgg ctagaaaagg acctcgaggt cgcgctgcta
      361 taccgttcga cgcgccgcct gtcgctaacc agcgaaggtc gcgaactcta cgagctgatg
      421 gcggcgctgg tcgccggcgc ggagcgcggc gtcgaccagt tgactgcggc cctgtcggaa
      481 cctgtcggcc ggctccaggt cacgctggca gcctcgtttt cacgcgagaa catcacgcga
      541 cagattgccg ccttcgcgcg ccagcacccg cgcgtagaaa tgtcggtgcg tttttcggac
      601 gagcaaaagg acctgattgc ggacggtatc gatctcgcga tccgcagcgg cccgattgcc
      661 gacagttcgt tgcaggcgag gaaactgttt tcgctcgaac gcattctggt cgcttcccca
      721 cgctacttgg cgagaatgcc gcgcgcggaa cagcccgacg acctggccga ctgggactgg
      781 atctggctcg atgttaccgc cgagcaccgc gagttccggc accgccagag tggcgaacgc
      841 cagaacgtga agttgaacaa gcgggtgatc gtcaacgacg gctacgcgat gtgcgaattc
      901 gcggtcgagg gtctcggcgt gctgacctcg cctgattacc tggtcgagga atacctgcgc
      961 caggggcagc tggtggaggt cctgcccgac tggcgtctcg ccccgatcga tgtctacgcc
     1021 atctggccgc cgaattcgcc gcgcaccggt ctaacgcgac gcttcgtcga tcatttgctg
     1081 gaacaccgat agtatccggt gagaatcaag aggcctactt ctttggcgcg caaaaccagt
     1141 atactgtgct ccgtttttct cccggattgt catgactgaa gatcgatcct atcccgaagt
     1201 gcgcgacgcg gtcgcgcgcc tgtgccgcga ttacccggac acctactggc gcgacaagga
     1261 ccgcgacaag gcgtacccgg aagaattcgt gcaggcgctg accgacgccg gttacctgtc
     1321 ggtgctgatt cccgaggaat acggtggcgc cggcatggac ctcggcgcgg cctgcgcggt
     1381 actcgaggaa atccagcgca gcggcgccaa cggcgccgcc tgccacgcgc agatgtatat
     1441 catgggcacg ctgctacgcc atggcagcga gcaacaaaag cagaaatatc tgcccgacat
     1501 cgccgccggc aggttgcgcc tgcaggcttt cggcgtcacc gagccgtcgg ccggcaccga
     1561 caccacgcgc atcaagactc gcgcggagcg cgacggcgac gatttcgtcg tcaacggtca
     1621 aaaggtattc atctcgcgcg tcgagcattc cgacctgctg ctgttgctgg tgcgcaccac
     1681 gtcgcgcgat gcggtcgaca agcccagccg gggcatgtcg gtgctgctgg tcgacctgcg
     1741 tgacgcgatc ggcaagggtc tcaccgtcaa accgctcgca accatgttga accatgccac
     1801 caccgaactg ttcttcgaca acctgcgggt gccggccgcc aacctgatcg gcgaggaagg
     1861 caagggcttc ggctacattc tcgacggcat gaacgcggaa agaatcctaa tcgccgccga
     1921 gtgtatcggc gacgcgcgct ggttcatcga ccgcgcgggc aactatgccg cgcagcgcga
     1981 ggtgttcggt atgccgatcg gcaaaaacca gggtatccag tttccgatcg cgcgcgctca
     2041 cgtgcagacc gaggcggcgg cgttgatggt cgacaaggcc tgccgcctgt tcgacgccgg
     2101 cgagccctgc ggctcggaag ccaacagggc caagctgcgg gccgccgacg cctcctgggc
     2161 ggcggccgac atgtgcctgc aaacccacgg cggctacggc ttcactgtcg agtatgacgt
     2221 cgagcgcaag tttcgcgaaa cccgcctcta ccaggtggcg ccgatctcga ccaacctgat
     2281 cctgtccaac atcgctaccc acgtgctcgg cctgccgaag tcgttttgat gctgaagctc
     2341 gacgacatcc tcgtggtctc gctggagcag gcggtggccg cgccattctg ttccaaccgc
     2401 ctggcccgtg ccggcgcgcg ggtgatcaag atcgagcgcg cggaaggcga tttcgcgcgc
     2461 ggctacgacg acgtggtgaa cggcgaatcg gcttacttcg tgtggctcaa ccagggcaag
     2521 gaatcgctgg tgctggattt caagcgggcg gccgacgccg aattgctgca ccggctgctg
     2581 gcgagtgccg atgtctttat ccagaatctc gcgcccggcg ccgcggcgcg tgccggcttt
     2641 ggcagcgacg cgctacgcga gcgttacccg cggctgatct gctgcgatat caccggttac
     2701 ggcgacagcg gcgaataccg ccgcatgaaa gcctacgacc tgctggtgca gtgtgaatcg
     2761 ggcatggcct cgatcaccgg cacgccggaa gcgccggggc gcgtcggcgt ctcggcctgc
     2821 gatatcaact gcggccagca ggcctatgcc gcgatcctcg aggcgctgat cgcccgcgaa
     2881 tccaccggca gcggctgcgt ggtcaaggtc tcgctgttcg acgcgatggc cgaatggctc
     2941 gcggtgccgc tgctgcatta cgactacggc ggccgcgtcc agggccgcgt cggcctcaac
     3001 cacgcgacga tttcgc
//
LOCUS       DATCZV010000162         9777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_541492, whole genome shotgun sequence.
ACCESSION   DATCZV010000162 DATCZV010000000
VERSION     DATCZV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541492"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="ppk1"
                     /locus_tag="VIW27_03555"
     CDS             complement(<1..1592)
                     /gene="ppk1"
                     /locus_tag="VIW27_03555"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY5738774.1"
                     /translation="MVAPKAEKTLSIVENREPQIPDDAAQTPPVADSQAAPGRYQDLD
                     APELYLNRELTWLEFNRRVLHEAQDERNPLLERLKFVGIVSSNIDEFFMKRIGGLKQQ
                     VGAGVMDFSIDGRLPAEQIAECYVKVREIEAQKDALVPELIGQLAEQGIYIKPYDELG
                     DADKETIRNYYFDNIYPLVTPQGVDSAHPFPFISNLSLNLLVSVRHRDSEDEMRARVK
                     VPVGLGIPRFLQVPNTFIFVPLESVMAKNLDVLFPKMDIIACETFFVIRNANTARDED
                     QADDLLAMIESEVRHRRFAKIVRVVVQSDMDSTRRGMLAAELGLDEADVFERHGIQSA
                     RDLLSLTDLEIPALRDPPHHPIDHPKLVHMPNIFHAVRDIGSILLHHPYESFSTSVER
                     LLREASRDPKVRAIKMTLYRTSGDTKVIDYLVDAAQNGKQVAVVVELKARFDEAANIR
                     WSSRLEEAGIHVTYGVMELKTHCKVILVVRQDYSGLRRYVHIGTGNYHAGNARLYTDL
                     GLLTSDPDIGRDITELFNYLTTG"
     gene            complement(1618..2436)
                     /gene="ppk2"
                     /locus_tag="VIW27_03560"
     CDS             complement(1618..2436)
                     /gene="ppk2"
                     /locus_tag="VIW27_03560"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY5738775.1"
                     /translation="MNDTANAQAENKKRNKISNKEYDKELAELQIELVKLQEWIKEKG
                     LKVVCIFEGRDAAGKGGVIKRITESLNPRICRVVALGTPTEREKTQWYFQRYVPHLPA
                     AGEMVLFDRSWYNRAGVERVMGFCSEHEYEEFMRSCPEFERMLVRSGIILIKYWFSVS
                     DEEQERRFQGRIKDPTKRWKLSPMDVESRNNWVEYSKAKDQMFAHTDIKQAPWYVVNA
                     DVKKHARLNCIKHLLSMIPYEDLTPEPVELPPREKAGGYVRPPLNDQTFVPEHH"
     gene            2640..4199
                     /locus_tag="VIW27_03565"
     CDS             2640..4199
                     /locus_tag="VIW27_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5738776.1"
                     /translation="MNLSVLLAERAARLGDRDFLLFEHQSGDGEADGFRQRLSYAELE
                     ASVNRACRMLAAHGLEAGEVINLHLPNCPAFVILWFAAARLGAVMMPTNVLASADELT
                     YLLEHSQSKIAFTTRQHSPVVEQCRANYGGLREIMLCDPCSAQPAAGSFEAELPSYPD
                     HSSECPAADTSLAAIMYTSGTTSKPKGVMVTHANYITAGRTVAGALELDEHDRQFVVL
                     PLFHGNAQYYSVMSALLQGASVALMDRFSASRYFDKCIEYQCTVASLFAAPMRMILAQ
                     PENPAHRENALRVVIFAQNLTAAQLDDWQRRFDAPLCQLWGMTETMGPPLMNPLHGER
                     RNWTVGKPAGDYRIALVDEQGQQLDAGREGEIAVSGEPGRTIMAGYFRNPTATDETIR
                     DGWLYSGDNAVRDEDGFYRFVDRKKDMIKRAGENVSAGEVESVVLQHPAVFECAVVGM
                     PDEVRDEEIVAVVVTRPGAVLGDAELIEFCAARLAKFRVPGRILFTDSLPKTSVGKIQ
                     KHLLRESLSDA"
     gene            4322..5686
                     /locus_tag="VIW27_03570"
     CDS             4322..5686
                     /locus_tag="VIW27_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5738777.1"
                     /translation="MSKTFAPVAALLLAVALLLTGNGLLGTLIPVRAQLEDFSIFAIG
                     LLGSSYFIGFGAGCFYGPHLLRRAGHIRVFTAMASIVSAVVLGHGLMAIPFPWWLMRG
                     ITGFCFAVLYIVIESWLNEASSNETRGKVLSAYLVINLTVITVGQMMLTLDDPAEFEL
                     FALASILVSISVVPIALTAARAPAQIETVHLRIGKLFETSPIAFVGCIGVGLANGAFW
                     TLSPLFVQRAGYDIEAVALVMSATVLGGAIGQWPIGRLSDRMDRGVLLGWAALAAGVL
                     SVGVIIASALSVGVLLVAAAAWGALSFPLYAVAVAQANDHAQPSEYVEVSGGLLFAFA
                     AGAVVGPLLATVFMDLIEVGGLYVFTSSVQVALAMFVYRQIKRGKPLAETEHHLTMAE
                     ALQTAHTVSTVFDATLAQDESGNAQQETTGATEDSAMETVDRSDPPDVEPGPDPERPQ
                     DAGK"
     gene            complement(5705..7108)
                     /locus_tag="VIW27_03575"
     CDS             complement(5705..7108)
                     /locus_tag="VIW27_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY5738778.1"
                     /translation="MLRAAILAPLLILLTSVAPALEQNISSSVVKITTKTTVGGKIFV
                     ESATGWAWQKPTQVVTALHAVAGKKFIEVKNRQGQKSTAQIVRVVKQGDLALLQLDKD
                     LGLVPLETGPVQHNSPETFHVVGFPHNIPTLSADRIRFSTSFEGQPVLSHLIGGTKLE
                     NLLRSQGYPLPETYIYRLSDTIQPGHSGAPIVRENGIVIGVADGGLKKGTARINWAIP
                     ADHYVAQLDSSSETIPQLPSKQSELFSVKIEIESAMVEAPEIMESGSISDPSGQVTVH
                     KVWNASLEEIYFSLEESDQQDLEDIFEDFSEEEIEYLAQALRYDIYEDYNTGATFAVP
                     EGAEIVYEDEAFAVSLGQFEYDFIPQNTATFEEAVQTVRIFADELLKYYPNALQYEED
                     DEDGDADEQWYNLAKMRVIEEDGEQKFFAVGGEALGENAALIVFVSPLPEVLDEDQMI
                     RFMGFVVGLEIISFASY"
     gene            complement(7125..7961)
                     /locus_tag="VIW27_03580"
     CDS             complement(7125..7961)
                     /locus_tag="VIW27_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738779.1"
                     /translation="MQNNKRLVSVLSVLFAGLGMGLLIGLIMGLSISPTTKIVMGVLA
                     AGLGAFLGFDQRAFKASSSDNPQAGRSDLLRELRVGSFAFAVIAGILLGMTIRTNELL
                     LPTISESVKKWTDAGYTVEVARQFVALERLRIDPKTGEVRDLTDADKSRITALFSSQK
                     QIAFSHLIRPKEWAADMDSAIAEARALELSDLENLLTAIYNNAPEESRELLLAGIKQL
                     AEHVESGSRFACSLADEPEQWRNEAVAGILPLLMAIDSPNERSAIGRATRALLCKLES
                     SL"
     gene            complement(8306..9115)
                     /locus_tag="VIW27_03585"
     CDS             complement(8306..9115)
                     /locus_tag="VIW27_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5738780.1"
                     /translation="MTTQPLLVLGIRIAAIASRRSASLLSVIVLSTAGMAIWGPAGAA
                     DPQAHEFTCSSCHLAEVVDATNASNLVGEESALCQTCHQNSVTASHPVGVRPQVPPPD
                     AFPLNASGEITCSTCHRVHDPAPGKLRTTLEGQSFCEACHQPSFFSGMKDEGISLMAL
                     GHLDASASLSGDIDNFSIQCMSCHDKSGDALRMPLTVAGSERRNHPIGSRYNDSISFG
                     SYRPVAGLPENILLPDGKLSCVSCHVGYSEQHGALVVDNRGDRLCQSCHAL"
     gene            complement(9133..9390)
                     /locus_tag="VIW27_03590"
     CDS             complement(9133..9390)
                     /locus_tag="VIW27_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738781.1"
                     /translation="MKQANCGGGHNTLNGTWRENREHPSGGMHRMAITASGGNSIDSI
                     LIDEVDQGAAGVLTAEAANIRESHSPMALKAASSWIAPENI"
     gene            complement(9550..>9777)
                     /locus_tag="VIW27_03595"
     CDS             complement(9550..>9777)
                     /locus_tag="VIW27_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738782.1"
                     /translation="HRCAVSGMTFRVEEKLHHSGQRSAATSRLNDLIRRALCLGSDPV
                     VAANISNAVARNDPNPEGMAWEMQEHASRIA"
BASE COUNT         1733 a         2955 c         3079 g         2010 t
ORIGIN      
        1 ccggtggtca ggtaattgaa cagttcggta atgtcgcggc cgatgtcggg gtcgctggtc
       61 agtagcccaa ggtcggtgta cagccgtgcg ttgccggcat ggtaattgcc ggtgccgatg
      121 tgcacgtagc ggcgcagccc gctgtaatcc tggcgtacca cgaggatgac cttgcagtgt
      181 gtcttcaact ccatcacgcc gtaggtgacg tggatgccgg cttcctccag gcggctcgac
      241 cagcgtatgt tggcggcttc gtcgaagcgc gccttgagtt cgaccaccac cgcgacctgc
      301 ttgccgtttt gcgccgcgtc caccaggtaa tcgataacct tggtgtcgcc cgaggtgcgg
      361 tacagcgtca tcttgatcgc acgcaccttg ggatcgcggc tggcctcgcg cagcagccgc
      421 tcgaccgagg tcgaaaagga ttcgtaggga tggtgcagca ggatcgagcc gatgtcgcgc
      481 accgcgtgaa agatgttcgg catgtgcacc agcttgggat ggtcgatcgg gtggtgcggc
      541 ggatcgcgca gcgccggaat ctcgagatcg gtcaggctta gcaagtcgcg cgctgactgg
      601 atgccgtggc gctcgaagac gtcggcctcg tcgagcccga gctcggcggc gagcatgccg
      661 cggcgcgtcg agtccatgtc gctttgcacc accacgcgca cgatcttggc gaaacgccga
      721 tggcgcacct cggattcgat catcgccagc aggtcgtcgg cctggtcctc gtcgcgcgcg
      781 gtgttggcgt tgcgaatgac gaagaacgtt tcgcaggcga tgatgtccat tttcggaaac
      841 agcacgtcga ggttttttgc catcacgctt tccagcggca cgaatatgaa tgtgttcggc
      901 acctgcagga aacgcggaat acccagcccc accggcacct tgacgcgcgc gcgcatctca
      961 tcctcgctgt cgcgatggcg caccgagacc agcaggttca gcgacaggtt ggagatgaac
     1021 gggaacgggt gcgcggagtc gacaccctgc ggcgtgacca gcgggtagat gttgtcgaag
     1081 taatagttgc gaatggtctc cttgtcggca tcgccgagct cgtcgtaggg cttgatgtag
     1141 atgccctgtt cggcaagctg gccgatcaac tcgggtacca gtgcgtcttt ctgcgcctcg
     1201 atttcgcgta ccttgacgta gcattcggcg atctgctcgg cgggcaggcg gccgtcgata
     1261 ctgaaatcca tgaccccggc acccacctgc tgcttgaggc cgccgattcg tttcatgaaa
     1321 aattcgtcga tattcgagct gacgataccg acgaacttga gccgctcgag cagcgggttt
     1381 ctctcgtcct gcgcctcgtg caacacgcgg cggttgaact cgagccaggt cagctcgcgg
     1441 ttgagataca gctcaggcgc atcgaggtcc tggtatctgc cgggagccgc ctgtgaatcg
     1501 gcgaccggcg gcgtttgtgc ggcatcgtcg ggaatttgcg gctcgcggtt ttcgacaatc
     1561 gacagggtct tttcggcttt cggggcaacc atgctgttta ttccttttga ttttgctcta
     1621 gtggtgttcg ggaacgaagg tctggtcgtt cagcggcggg cgcacgtagc caccggcttt
     1681 ttcgcgcggc ggcagctcga ccggttccgg ggtcaggtct tcgtagggaa tcatgctcaa
     1741 caggtgcttg atacagttga gacgcgcgtg cttcttgacg tcggcattga ccacgtacca
     1801 gggcgcctgc ttgatatcgg tatgcgcgaa catctggtcc ttggcctttg aatactcgac
     1861 ccagttgttg cgcgactcga cgtccatcgg gctcagtttc cagcgcttgg tcggatcctt
     1921 gatgcgcccc tggaagcggc gttcctgctc ttcgtcgcta accgaaaacc agtacttgat
     1981 caggatgata cccgagcgca ccagcatgcg ctcgaactcg gggcagctgc gcatgaattc
     2041 ctcgtactcg tgctcggaac agaaacccat cacccgctcg accccggcgc ggttgtacca
     2101 gctgcggtcg aacagcacca tctcgccggc ggcaggcaga tgcggcacgt agcgctggaa
     2161 ataccactgg gtcttctcgc gttcggtcgg cgtgccgagt gcgactacgc ggcagatacg
     2221 cgggttgagg ctctcggtga tgcgcttgat gacgccgcct ttgccggccg cgtcgcggcc
     2281 ctcgaagatg cacactacct tgagcccctt ttccttgatc cactcctgca gcttgaccag
     2341 ctcgatctgc agctccgcga gctccttgtc gtattccttg ttggagattt tgttccgttt
     2401 tttattctcg gcctgcgcgt tagctgtatc gttcattacc gctctccccg tttgtcatag
     2461 cttgacccac caacctataa caaacgctaa accctgtcca gaaaccgggc gacgaatttg
     2521 ccgcccgata ccgcgaccgg ccgatcgcgg ttgcctggcc gggtgctgag gtaaccgttg
     2581 atcgcttgac cgtgatcgct aaatgctaaa ttatagtccg cgtgagcgct gcatcgacga
     2641 tgaatctgtc cgtgctgctc gccgagcggg cggcgcgcct cggcgacagg gatttcctgt
     2701 tgttcgagca ccagtccggc gacggcgagg ccgatggctt ccgccagcga ttgagttatg
     2761 ccgaactgga ggcgagtgtc aatcgcgcct gccggatgct cgcggcacac ggcctcgagg
     2821 ccggcgaggt gattaacctg cacctgccca attgcccggc cttcgtcatc ctctggttcg
     2881 ccgccgcgcg cctcggcgcg gtgatgatgc cgaccaacgt gctggcgtcg gccgacgagc
     2941 tgacctacct gctggaacat tcgcaatcga aaatcgcctt taccacccgc caacactcgc
     3001 cggtagtcga gcaatgccgg gcgaactatg gcggcttgcg cgaaatcatg ctttgcgatc
     3061 cctgcagcgc gcaacccgcc gccggcagct tcgaggccga gctgccgtct tacccggacc
     3121 actcctcgga atgtccggcc gccgacacca gcctggcggc gatcatgtat acctcgggca
     3181 ctacctcgaa accgaagggc gtgatggtca cccatgccaa ttacatcacc gcggggcgta
     3241 ccgttgccgg tgcgctcgaa ctcgacgagc acgaccggca gttcgtcgtg ctgccactgt
     3301 tccacggcaa tgcgcagtat tactcggtga tgagtgcatt gttgcagggt gccagcgtgg
     3361 cactgatgga ccggttttcg gcgagccgtt atttcgacaa gtgcatcgag taccagtgca
     3421 ccgtcgccag cctgttcgcc gcgccgatgc gcatgatcct ggcgcagccg gagaatcccg
     3481 cgcaccgcga gaacgcgctg cgggtggtga tcttcgcgca aaacctgacc gcggcgcagc
     3541 tcgacgactg gcagcggcgt ttcgacgcgc cgctgtgcca gctgtggggc atgaccgaga
     3601 ccatgggacc gccgttgatg aatccgttgc acggcgagcg gcgcaactgg accgtcggca
     3661 agcccgcggg tgattaccgg atcgcgctgg tcgacgagca gggccaacag ctcgacgccg
     3721 ggcgagaagg cgagatcgcg gtcagcggag agccgggccg cacgatcatg gcgggttact
     3781 tcaggaatcc gacggccacc gacgagacga ttcgcgacgg ctggctgtac agcggtgaca
     3841 acgcggtacg cgacgaagac ggcttttacc gcttcgtcga tcgcaagaag gacatgatca
     3901 agcgcgccgg cgaaaacgtc tccgccggcg aagtcgagag cgtggtgctg cagcatccgg
     3961 cggtattcga gtgcgcggtc gtcggcatgc ccgacgaagt ccgcgacgag gaaatcgtcg
     4021 ccgtggtcgt gacccggccc ggcgcggtgc tcggcgacgc cgaactgatc gagttttgtg
     4081 ccgcgcggct ggcgaaattc cgcgtgccgg gacgcatcct gttcaccgac tcactgccga
     4141 agacctcggt cggcaagatc cagaaacacc tgctgcgcga gtcgctgtca gacgcttaat
     4201 ccgggcggcg ggcagattcg acaatcgcga cgacagaaac tagactctag agtcaaaagc
     4261 ggaacgctcg tccgggaaca ggtgccggcg ggagaattcc gccgctcagg gataactgac
     4321 aatgtcgaaa acctttgcgc cggttgctgc gctgttgctc gcggttgccc tgctgctaac
     4381 cggcaacggc ttgctcggca cgctgattcc ggtgcgtgcg cagctggaag atttctcgat
     4441 cttcgccatc ggcctgctcg gctcgagcta tttcatcggc ttcggcgccg gctgtttcta
     4501 cggcccgcac ctgctgcgcc gcgccgggca tattcgcgtc ttcaccgcga tggcgtcgat
     4561 cgtctccgcc gtggtgctgg ggcacggcct gatggcgatt ccgttcccgt ggtggctgat
     4621 gcgcggcatc accggcttct gtttcgcggt gctttacatc gtcatcgaaa gctggctcaa
     4681 cgaggcctcc agcaacgaga cccgcggcaa ggtgttgtcg gcctacctgg ttatcaacct
     4741 gaccgtgatc accgtcgggc agatgatgct gaccctcgac gatccggcgg agttcgaact
     4801 gttcgcgctg gcctcgatcc tggtatcgat ctcggtggtg ccgatcgcgc tgaccgcggc
     4861 gcgcgcgccg gcgcaaatcg aaaccgttca cctgcgtatc ggcaagctgt tcgagacttc
     4921 gccgatcgcg ttcgtcggct gtatcggcgt cggtctcgcc aacggcgctt tctggacgct
     4981 gtcgccattg ttcgtgcagc gcgcgggtta cgacatcgaa gcggtggcgc tggtgatgag
     5041 tgctaccgtg ctcggcggcg ccatcggcca atggccgatc gggcggctgt ccgaccgtat
     5101 ggaccgcggc gtgctgctcg gctgggccgc gctcgcggcc ggcgtgctgt cggtcggcgt
     5161 gatcatcgcc agcgcgctgt cggtcggcgt actgctggtg gccgccgcgg cctggggcgc
     5221 gctgtcgttc ccgctgtacg cggtcgccgt cgcgcaggcc aacgatcacg cgcagccgtc
     5281 cgaatacgtc gaggtgtccg gtggcctgct gttcgccttc gccgccggcg cggtggtcgg
     5341 gccactgctg gcgaccgtgt tcatggatct gatcgaagtc ggcggattgt acgtgttcac
     5401 ctccagcgtg caggtggcgc tggcgatgtt cgtttaccgg cagatcaagc gcggcaagcc
     5461 gctggccgag accgagcatc acctgacgat ggccgaagcc ctgcagaccg cgcacacggt
     5521 atcgacggta ttcgacgcca cgctggcgca agacgagagt gggaatgcgc agcaggagac
     5581 taccggtgcg acggaagata gcgcgatgga gaccgttgac agatccgacc cgccagacgt
     5641 cgagccaggt ccggatccgg agcgaccgca ggacgccggc aaataggccg gggacccgct
     5701 ggcttcagta gctcgcgaac gaaataattt ccaaaccgac gacaaaaccc atgaagcgga
     5761 tcatttgatc ctcatcgagg acttcgggca gcggactgac aaaaacgatc aacgccgcgt
     5821 tctcgcccag cgcttcgccg ccaaccgcga aaaacttttg ctcgccgtct tcctcgatga
     5881 cgcgcatttt ggcgaggtta taccactgct cgtcggcgtc cccgtcttca tcgtcttctt
     5941 cgtactgcag cgcgttcggg tagtatttca gcagttcgtc ggcgaaaatc cgcaccgtct
     6001 gcacggcctc ttcgaaggtc gcggtatttt gcgggataaa atcgtactcg aactgcccga
     6061 ggctgaccgc gaaggcttca tcctcgtaaa ctatctcggc cccttcgggc accgcaaagg
     6121 tggcgccggt gttgtagtcc tcgtagatat cgtagcgcag agcctgggcc aggtattcga
     6181 tctcttcctc ggagaaatct tcgaatatgt cctccaggtc ctgctggtcg gattcttcca
     6241 acgaaaaata gatttcctcc agcgatgcat tccacacctt gtgcacggtg acctggccgc
     6301 tcggatcgga aatgctgccg ctttccatga tctccggtgc ctcgaccatg gcggattcga
     6361 tctcgatctt gacgctgaac aactccgatt gtttggaagg cagctgcggt atcgtttcac
     6421 tgctgctgtc gagctgggcg acatagtggt cggccggaat cgcccagttg attcgcgcgg
     6481 tgcctttttt cagaccaccg tcggcgacgc cgatgacgat gccgttttcg cgtacgatcg
     6541 gcgcgcccga gtggccaggt tgaatggtat cggagaggcg gtagatatag gtttccggca
     6601 gcgggtaacc ctggctgcgt aacaggttct cgagcttggt gccgccgatc aggtgactca
     6661 ggacaggctg accctcgaag gaggtcgaaa agcgaattcg atccgccgac agggtcggga
     6721 tgttgtgcgg gaaaccgacg acgtgaaagg tttcgggcga attatgctgc accgggccgg
     6781 tctcgagtgg aaccaggccg aggtccttgt cgagctgcag cagggcgaga tcgccctgct
     6841 tgacgaccct gacgatttgc gccgtggatt tttgcccctg ccggtttttg acctcgatga
     6901 atttttttcc cgccaccgca tgcagcgcgg tcaccacctg ggtcggtttt tgccaggccc
     6961 agccggtggc ggattcgacg aagatcttgc cgccgaccgt ggtcttggtg gtgatcttga
     7021 ccaccgaact ggaaatgttt tgctcgagcg ctggcgccac ggacgtcagc agtatcagca
     7081 gtggggcgag aatcgcggca cgtaacattc gttgtttcct cccgttacag gctcgactcg
     7141 agcttgcaga gcagtgccct ggttgcccgg ccgatagccg atctttcgtt cgggctgtcg
     7201 atggccatca atagcggcaa tattccggca accgcctcgt tgcgccactg ctccggctcg
     7261 tcggccagcg agcaggcgaa gcggctgccg ctctcgacgt gttcggccag ctgcttgata
     7321 ccggcgagca ggagctcgcg gctttcttcc ggcgcgttgt tataaatcgc ggtgagcagg
     7381 ttctcgaggt cgctcaactc gagcgccctg gcctcggcga tggcgctatc catgtcggcg
     7441 gcccactcct tcggccggat caagtggctg aaggcaattt gtttctgcga agagaaaagc
     7501 gcggtgatac gcgacttgtc ggcatcggtc aggtcgcgca cctcgccggt tttcggatct
     7561 atgcgcaggc gttccagcgc cacgaactgg cgtgcgactt cgaccgtgta cccggcgtcg
     7621 gtccattttt ttacgctttc gctgatcgtc ggcagcagta attcattggt gcgaatagtc
     7681 attccgagca ggatgccggc aatcaccgcg aaggcgaagc tgccgacgcg taactcgcgc
     7741 agcagatcgc tgcggccagc ttgagggttg tcgctggagg aagctttgaa tgcgcgctgg
     7801 tcgaagccga ggaacgcgcc gagaccggcg gccagcacgc ccatcacgat cttcgtcgtc
     7861 ggtgaaatcg acagacccat gatcaggccg atcagcagcc ccatgccgag gccagcgaag
     7921 agtacggata agacggatac cagccgttta ttgttttgca atgcatgctc gccggtatga
     7981 ggacgcggaa aagtatattt aggcgacccc agataagcaa gcaaagcctt ggggctggaa
     8041 agctctcgaa atttcaacag gatgggtgaa tctaccccgg ggccggcaac tgcctacaga
     8101 gtgggcggcg gaatccggcg ggcagcgttg ccggacgaat cggctcctcc aggctgacgt
     8161 gatccggctg gccgtcgtgc cgggctctgg tcaggcgatt gccgtctttt atggtgggta
     8221 tgcgaggccg gcatgaatgt ggtcgaacgg atggcgggca gcaacgcggg caagcggggc
     8281 ggggaatgcc gtgggcggcg cctggctaca gtgcgtggca actctgacac aggcggtcgc
     8341 cgcgattatc gacgaccagc gctccgtgct gctcgctgta gccgacgtgg cagctgacac
     8401 aactgagttt gccgtcgggc agcaggatgt tctcgggcaa tccggcgacc gggcgataac
     8461 tgccgaaact gatgctgtcg ttgtagcggc tgccgatcgg atgattcctg cgctcgctac
     8521 cggcaaccgt gagcggcatg cgtaacgcat cgcccgattt atcgtgacag gacatgcact
     8581 gaatcgaaaa gttgtcgata tcgccgctca gcgacgcact ggcgtcgagg tgaccgaggg
     8641 ccatcagcga aatgccctcg tccttcatgc cgctgaaaaa gctcggctga tggcaggctt
     8701 cgcaaaagct ctggccctcg agggtagtgc gcagcttgcc gggcgccggg tcgtgcactc
     8761 ggtgacaggt actgcaggtg atttccccgc tggcattcaa cggaaacgcg tcgggcggcg
     8821 gtacctgcgg ccgaaccccg accgggtgac tggcggtgac cgagttttga tggcaggtct
     8881 ggcacaaggc actttcttcg ccgaccaggt tgctggcatt ggtggcgtcg acaacctccg
     8941 caagatgaca actgctgcag gtgaactcgt gcgcctgcgg gtcggcggcg ccggccgggc
     9001 cccagatggc catgccggcc gtcgacagca cgatgaccga cagcaggctg gccgagcggc
     9061 ggcttgcgat cgccgcaatc cgaatcccga gcactaagag aggctgtgta gtcatcgaag
     9121 ccctggtgaa ggtcagatgt tttctggcgc tatccacgat gaagccgcct tcagtgccat
     9181 cggtgaatgt gattcacgaa tattcgccgc ctcggccgtc agcacaccgg ccgcgccctg
     9241 atcgacctcg tcgataagaa tcgagtcgat gctgttaccg cccgatgcgg taatggccat
     9301 tctgtgcatg ccgccggacg gatgctcgcg attctcacgc caggttccat tgagtgtatt
     9361 gtggccaccg ccgcaatttg cctgcttcat ttcacaccgc ccattcggtc ttctgatgtt
     9421 gaagattttt ccagaagcgc aaagaaaaaa atgtggtaaa taccacctgc gacagtaaaa
     9481 acgtcctcgt cggctactcc ttgtatacgc acccgacgcc cggggggatt actcgaagcc
     9541 gacaaaccgt caagctattc ggcttgcgtg ctcctgcatt tcccacgcca ttccctcggg
     9601 attcggatcg tttctggcta cggcattgga gatattcgct gccaccaccg ggtcgctgcc
     9661 caggcagagt gctctgcgga taagatcatt cagccgcgac gtcgctgcgc tccgctgtcc
     9721 cgagtggtga agtttctcct caactcggaa agtcatcccg gaaaccgcgc agcgatg
//
LOCUS       DATCZV010000163         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_170837, whole genome shotgun sequence.
ACCESSION   DATCZV010000163 DATCZV010000000
VERSION     DATCZV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170837"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2274
                     /locus_tag="VIW27_03600"
     CDS             <1..2274
                     /locus_tag="VIW27_03600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5738783.1"
                     /translation="LDKVLTTITQVGEPHSEVLKNICRESADGFSEAYFLDVLSHDET
                     EFRAAASEILSQSGSGDSSRLFQRLHEPGASLNEVIEVLEARKHLLKPEDIINHSLKL
                     ESDYAIQLLKLIEGSQQPVDLSNLSFQLDKIESPIYKINLLNYFGTVNEPKIPLIVSR
                     FLDDTNKVVVLEALKTLNRLKIDFDVSVLLPYAETMSGVELEYVLKIIAKQADASLVP
                     HLSAYLTSKSSELNDFFARIVVVNSDTENFEKFLRRLMLEDDWTQQQAVACVQKFANE
                     NISDVARELTGHDQEFVRNSAQALVMNLIGDEDLGRVEEFALSDNWQVRERAIQSLAK
                     SSNRGAIAILKKQVEAYPDDFVLVLRAVKQLGFGKGLEIAFDGLKSSHANVQRAALET
                     IEAITTEKHAINVRDNILWSLPMLTTEMREFAKMLMSQITRDFDLPDMQIDENSNTST
                     GAVDLPLFDATGSLRPVENISLLDALGPGEVWMDRYHIKEEIGRGAMGRVMLVEDDMV
                     DEKLVLKFMLPELTMDQDSTERFKREVKYARRVSHRNVIRVHDILLKDQVCAISMEYF
                     RSRGLEAVIKKINSFETVDGLSILYQIASGMAAAHEEEVIHRDLKPSNILIDDKWQVK
                     IVDFGIASAGTQSDSTLTQTGSIIGSPAYLAPERAEGADADERSDIYSLGVIAYYMFS
                     GKLPYVGKPMEVLSMHRAGDAPPIVKVKDGASPEVSRLIVKMMAVDPGARPQSMAAVR
                     DEVKALHDALELPEEIS"
     gene            complement(2311..>2696)
                     /locus_tag="VIW27_03605"
     CDS             complement(2311..>2696)
                     /locus_tag="VIW27_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016789651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY5738784.1"
                     /translation="VAMLSFSTDGSASHPLVDKVRRATELARRQMPGVAVEGEVQLDA
                     AIVPAIAAQKIKDSRIDGRANVLIFPDLDAGNIGYKLTQRFGGASAIGPVLQGLNRPA
                     NDLSRGCSTEDVYNLIALTAVQSRG"
BASE COUNT          585 a          766 c          810 g          535 t
ORIGIN      
        1 ctggataaag tgctgacgac cattacccag gtcggcgagc cgcatagcga agtgctgaaa
       61 aatatctgtc gtgaatcggc cgacggtttt tccgaggctt attttctcga tgtgttgtcg
      121 catgacgaaa ccgagttccg cgccgctgcc agcgaaatcc tgtcgcagtc cgggtcgggc
      181 gactcgagca ggttgttcca gcgtttacac gagccggggg cttcgctcaa cgaagtcatc
      241 gaagtgctgg aagcacgcaa gcacctgctg aaaccggaag atattatcaa tcactcgctc
      301 aaactggagt cggattacgc gatccagctt ttgaaactga tcgaaggcag tcagcagccg
      361 gtcgatttat cgaatttgag tttccagctg gacaagatcg aaagcccaat ttacaagatc
      421 aacctgttga attatttcgg cactgtcaac gagccgaaga tcccgctgat cgtctcccgc
      481 ttcctggacg ataccaacaa ggttgtcgtt ctcgaggcgc tcaagacgct gaaccggctg
      541 aagatagatt tcgatgtctc ggtactgttg ccctatgccg aaaccatgag cggtgtcgag
      601 ctcgaatacg tgctcaagat tattgccaag caggctgacg ccagcctggt gccgcatctg
      661 tcggcctatc tgacgtcgaa gtcgagcgag ttgaacgact tcttcgcgcg tatcgtagtg
      721 gtcaactccg acaccgagaa tttcgagaaa tttctacgcc gcctgatgct cgaggatgac
      781 tggacgcaac agcaggcggt tgcctgcgtg cagaagtttg ccaatgagaa catatccgat
      841 gtggcgcgcg aactgaccgg tcacgatcag gaattcgtgc gcaacagcgc gcaggcgctg
      901 gtcatgaacc tgatcggtga cgaggatctc ggaagagtcg aggaattcgc gttgagcgac
      961 aactggcagg tgcgggagcg cgcgatccag tcgctggcca agtcgtccaa ccgcggcgct
     1021 atcgcgattc tgaagaaaca ggtcgaggcc taccccgacg acttcgtact ggtgttgcgc
     1081 gcggtcaaac agctggggtt cggcaagggg ctcgagatcg ctttcgacgg gctcaagagc
     1141 tcgcatgcca acgtccagcg cgcggcgctg gaaactatcg aggcgatcac caccgaaaag
     1201 cacgcaatca acgtgcgcga caatattctg tggagcctgc cgatgctgac cactgaaatg
     1261 cgcgaattcg ccaagatgct gatgtcgcag attacccgtg atttcgacct gcccgatatg
     1321 cagatagacg aaaattcgaa taccagcacc ggggcggtcg atttgccgct gttcgatgct
     1381 accggttccc tgaggccggt ggaaaatatc tcgctgctgg atgcactggg tcccggcgag
     1441 gtgtggatgg atcgctatca catcaaggag gaaatcgggc gcggcgcgat ggggcgcgtt
     1501 atgctggtcg aggacgatat ggtcgacgag aaactggtac tcaagttcat gctgcccgag
     1561 ctaaccatgg accaggattc caccgagcgt ttcaagcgcg aggtcaagta cgcccgccgg
     1621 gtaagccatc gcaacgtcat tcgcgtgcac gatatcctgc tcaaggatca ggtctgcgcg
     1681 atctcgatgg agtatttcag gagtcgggga ctggaagcgg tgataaaaaa gatcaacagt
     1741 ttcgagaccg tcgatggtct cagtattctt taccagattg ccagcggcat ggcggccgcg
     1801 cacgaagagg aagtgatcca tcgcgacctg aagccgagta acatcctgat cgacgacaaa
     1861 tggcaggtca agatcgtgga tttcggtatc gcctcggctg gcacccagtc agattctacg
     1921 ctgacccaga ccggttcgat tatcggcagc cccgcctacc tcgcgcccga acgcgccgag
     1981 ggcgccgacg ccgacgagcg cagcgatatt tattcgttgg gcgtcatcgc ctactacatg
     2041 ttcagcggca agttgcccta tgtcggcaag cccatggagg tgctgtcgat gcatcgcgcc
     2101 ggcgatgcgc cgccgatcgt caaggtcaaa gacggcgcct cgcccgaggt ctcgcggctg
     2161 atcgtgaaaa tgatggcggt cgaccccggt gcgcgcccgc agtcgatggc cgcggtgcgc
     2221 gacgaagtca aggcgctgca cgacgcgctg gaactgcccg aagagatttc ctgaaattcc
     2281 ccgatcccgt cgaaccgacc cgggattaac ctagccccgt gactgcaccg cggtcagcgc
     2341 gatcaggttg tagacgtctt cggtactgca gccgcgcgac aagtcgttgg ccggacggtt
     2401 gagtccctgc agaaccggac cgatcgcgct tgccccgccg aatctctggg tcagcttgta
     2461 accgatattg ccggcgtcga gatcgggaaa aatcagcacg ttggcgcggc cgtcgatacg
     2521 cgaatctttg atcttttgcg cggcaatcgc gggcacgatg gcggcgtcga gctgcacttc
     2581 tccctccacc gcgacgccgg gcatctgccg gcgcgcgagc tcggtcgccc ggcgcacctt
     2641 gtccaccagc ggatgactgg cgctgccgtc ggtggaaaag gacagcatcg cgacac
//
LOCUS       DATCZV010000164         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_330121, whole genome shotgun sequence.
ACCESSION   DATCZV010000164 DATCZV010000000
VERSION     DATCZV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330121"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..451)
                     /locus_tag="VIW27_03610"
     CDS             complement(233..451)
                     /locus_tag="VIW27_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738785.1"
                     /translation="MTNYPEEARKDGSGGSDDTPRRRSTDLPPILDYSTRELVKNIVD
                     NKPLVDDKPSGEIRRKSLLRKFLDRGTK"
     gene            682..1095
                     /locus_tag="VIW27_03615"
     CDS             682..1095
                     /locus_tag="VIW27_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5738786.1"
                     /translation="MSSNRIQPGLRADTEILVGSRDTAHHVGSGKIKVLATPVMIMLL
                     EEAALNAVEDLLPRGYQTVGTRLDISHTAATPVGMRVTAHAELVAVDGRKLTFRVWAE
                     DEIELIGEGTHERIVVEVERFDRRTRAKSGGAPDA"
     gene            1133..1297
                     /locus_tag="VIW27_03620"
     CDS             1133..1297
                     /locus_tag="VIW27_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738787.1"
                     /translation="MLGDLNFGSGIVQIAEIPGDSQQFQDRMRFAADDDRLLRFHVAL
                     VESPLRSVLQ"
     gene            complement(1848..>2058)
                     /locus_tag="VIW27_03625"
     CDS             complement(1848..>2058)
                     /locus_tag="VIW27_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY5738788.1"
                     /translation="GVKCLSIDLAERGIGVISLHPGWVKTDMGGPNAEIGSDHSAAGL
                     KAILQSAGPEHSGGFFNYDGSPIPW"
BASE COUNT          383 a          641 c          631 g          403 t
ORIGIN      
        1 tcggaccgct cgccagcaac ctgctgcgtc accggccgct gaagacgggc aaacccgagg
       61 cgccaccgga agcggcggat gcggcacagg ctgcctccga accggcttcg ctgcgcgact
      121 agtcgcgggc agcggcaacg cgccgaccct cgttattcca ggtcccgata tcgagcaaga
      181 tcctgctcgc ggtccccggg gttatccagt cgaggccacc cgcttgcggc gattacttcg
      241 tgccgcggtc gaggaacttg cgcagcaagg atttgcgacg gatttcgccg ctgggtttgt
      301 catcgacgag gggtttgtta tcgacgatat tcttcaccag ttcccgggtc gaatagtcga
      361 gtatcggcgg caagtcggtc gagcgccggc gcggcgtatc atcactgccg ccggacccgt
      421 ctttgcgagc ctcttcgggg taattggtca tgtctgccat ccagggtaca actctccagc
      481 cgctaaatta cctgctcgca gccgccgatt tcgtgtccga attcacaacg acgacgattt
      541 aaaccgggtc gattgccggt aaatcgggcc ttgcagtgac agtttgcggg ctttacaggc
      601 agttgcggcc gagcggtccg gctggggatt cggcgcccgg ccgcgtatca tgcagccgtt
      661 gcccactgca tggatctccg catgtccagc aatcgaatcc agcccggcct gcgggccgac
      721 accgaaattc tcgtcggcag ccgcgatacc gcgcaccacg tcggcagcgg taagatcaag
      781 gtactggcga cgccggtgat gatcatgttg ctcgaggaag ccgcgttgaa cgcggtcgaa
      841 gacctgctgc cgcgcggtta ccaaaccgtc ggcacgcgcc tcgacatcag ccacaccgcg
      901 gcgacaccgg tcggcatgcg cgtcacggcg catgccgagc tggtcgcggt ggacgggcgc
      961 aagttgacat ttcgcgtctg ggccgaggac gagatcgaac tcatcggcga aggcacccac
     1021 gagcgcatcg tggtcgaggt ggaacgcttc gaccggcgca cccgcgcgaa atccggcggc
     1081 gcacccgacg cctgaagctc aatcgacgcc tgaaattcaa atcgacgaga atttgctcgg
     1141 cgatttgaat ttcggttctg gcatcgtcca gatcgccgaa atccctggcg atagccagca
     1201 gttccaggac cgtatgcggt ttgccgccga tgacgaccgt ctgctgcggt ttcacgtagc
     1261 ccttgtggaa tcgccgcttc gatccgttct gcaataaata ggtgccattg gtcgaatcga
     1321 gatcgcgcag gtagatcttc tggttggaga tccgaatctc ggcgtgatgg ttactcgccg
     1381 tgtttgaatc gatgaagata tgcccctggc gcccaatgat gaaggatcgg ccctcgacga
     1441 gcttgatgtt ctggtctgat tggttactcg gcattgttac ctcgtttcac ctggctgcac
     1501 tgatcgccaa accggcccgc gcaacgaatt ctcggacggc tgggggcaac gtacggccag
     1561 ctaaatgaga atagaacaaa ctgaacaatt ttcattaaat caggggcttg tgccgacgaa
     1621 cggagctccg ataccgtccc gttcggtagc cggcgaaaat cgctgcccgg gtgcaaatcg
     1681 ggcaggcgcc tgctgccgca ggccggctta aaggttattc cgggcggcga ccgccagccg
     1741 cgcgcggtcg aagcgcgtcg gcatggtcgc cggtcaccgc aggcaagcgg gtatcctgtg
     1801 ttctacccct gccccgttca cataggtcgc ggtgcagcgc cgtacggcta ccagggaatc
     1861 ggactgccgt cgtaattgaa aaacccgccg ctgtgctctg gcccggccga ctgcagaatc
     1921 gcctttagcc cggcagcgct gtgatcgctg ccgatttcgg cattcggccc gcccatgtcg
     1981 gttttgaccc atcccggatg caggctgatg acgccaatac cgcgctcggc gaggtcgatc
     2041 gacaggcact tgaccccc
//
LOCUS       DATCZV010000165         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_12398, whole genome shotgun sequence.
ACCESSION   DATCZV010000165 DATCZV010000000
VERSION     DATCZV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12398"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VIW27_03630"
     CDS             <1..632
                     /locus_tag="VIW27_03630"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY5738789.1"
                     /translation="FDLLELPTRVTDFLDPVQIKTVYEAEISEFLERVTGAYRVHQFD
                     HTVRASSPELREIKNLREPATLVHNDYTSKSGFTCLREQLGDEAEELLRGRFQIINVW
                     RPLSDPVEDYPLALVDARSVAPGNIVDTERRSPTHVGEIQLAVHDPGQRWYYYPAMRP
                     PEVLVFKTFDSIDGGRRSCTIHTAIKLPDAPPNAKPRESIETRALVFYA"
     gene            634..1761
                     /locus_tag="VIW27_03635"
     CDS             634..1761
                     /locus_tag="VIW27_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEY5738790.1"
                     /translation="MSTADTRRQALLEIWQAAIDAVAGRTAVIHALDDDDDFSPDLIV
                     AVGKAASGMCLGALERFPDCPALVVTKYDHSDAELRALPRVEVIESAHPIPDENSLRA
                     GRALLERIGGMPADSRVLLLVSGGASALAEALPDDMTLADLRAMTDKMIATGKTIGEI
                     NARRKQASLIKDGKLIATFDGAEIRVYAISDVEGDGIATIGSGIGDCHRAAGKARSTI
                     IASNSIAREQAERRARELGLEVGLNQEILYGDVFELAPKIAILLRDPEPGIYILGGEP
                     TVMLPDKPGRGGRNQSLALALAEHIAGRDDISILVAGTDGTDGPTTAAGGLVDGGTWH
                     EDARAALERADAGSFLDAHDALLVTGPTNTNVMDLVIAIVG"
     gene            1986..>2129
                     /locus_tag="VIW27_03640"
     CDS             1986..>2129
                     /locus_tag="VIW27_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5738791.1"
                     /translation="MKIAAIDHIVLRTSRLDDMLDFYCRVLGCQVERETGPEIGLVQL
                     RAGN"
BASE COUNT          404 a          680 c          687 g          358 t
ORIGIN      
        1 gattcgacct gctcgagttg ccgacgcgag tcaccgattt tctcgatccc gtgcagatca
       61 aaacggtcta tgaggcggag ataagcgaat tcctggagcg ggttaccggc gcataccgcg
      121 tgcaccagtt cgaccacacg gtgcgcgcct cgtcgccgga actgcgcgaa atcaagaacc
      181 tgcgcgaacc ggcgacactg gtgcacaacg attacacgtc gaaatcgggt tttacctgcc
      241 tgcgggagca actcggcgac gaggccgaag aactgttgcg gggccgcttt cagatcatca
      301 atgtctggcg gccgctgtcg gacccggtcg aggactaccc gctggcgctg gtcgatgcac
      361 gcagcgtcgc gcccgggaac atcgtcgaca ccgagcgccg ttcgccgacc catgtcggtg
      421 agatccagct tgccgtgcac gatcccgggc agcgctggta ttactacccg gcgatgcgcc
      481 cgcccgaagt gctggtgttc aagaccttcg actcgatcga cggtggtagg cgcagttgca
      541 cgatccacac cgcgatcaag ctgcccgatg caccgcccaa tgcgaagccg cgagagagca
      601 tcgagactcg cgccctggtg ttttacgcct gagatgtcca ccgccgacac acgccggcag
      661 gcgctgctcg aaatctggca ggcggcgatc gacgccgtcg ccggccgcac ggcggtgatc
      721 catgcgctcg acgacgacga cgacttcagc cctgacctga tcgtcgcggt cggcaaggcg
      781 gcttccggca tgtgcctcgg cgcgctggag cgttttcctg actgccccgc actggtggtc
      841 accaagtatg atcacagcga tgccgagttg cgcgcgttgc cgcgggtcga ggtgatcgaa
      901 tccgcccacc cgatccccga cgaaaattcg ctgcgagcgg gcagggcgct gctcgaacgt
      961 atcggcggaa tgcccgccga cagccgcgtg ctgctgctgg tctcaggcgg agcgtcggcg
     1021 ctcgccgagg cgctgcccga cgacatgacg ctggccgacc tgcgcgcgat gaccgacaaa
     1081 atgattgcca ccggcaagac catcggcgaa atcaatgcgc gccgcaagca ggcttcgctg
     1141 atcaaggacg gcaagctgat cgcgaccttc gacggcgccg agattcgggt atatgcaatt
     1201 tccgacgtcg agggcgacgg catcgcgacc atcggctcgg ggatcggcga ttgtcaccgc
     1261 gcggccggca aggcgcgatc gacgatcatc gcctccaaca gcattgcccg cgagcaggcc
     1321 gaaaggcgag cccgggaact cgggctcgaa gtcgggctga accaggagat cctctacggc
     1381 gacgtgttcg agctggcgcc gaaaatcgcc atcctgttgc gcgaccccga acccggaatc
     1441 tatattcttg gcggcgagcc gaccgtgatg ctaccggaca agcccggccg tggcgggcgt
     1501 aatcagagcc ttgcgctggc actcgccgag catatcgcag gccgggacga catcagcatc
     1561 ctggtcgccg gcaccgacgg taccgacggc ccgaccacgg ccgccggcgg cctggtcgac
     1621 ggtggcacct ggcacgaaga cgcgcgcgca gcgctcgagc gcgccgatgc cggcagcttt
     1681 ctcgacgcgc acgacgcgct gctcgtgacc gggccgacca ataccaatgt catggatctc
     1741 gtcatcgcca tcgtcggttg atgacgaata gaggcgcagt cgcaagccga ataacacatt
     1801 cgcgcgattg tcgagttaca atccgtgatg gaggatggcg ttttgtggct gaaggctatc
     1861 gatatgagga gatctcgcac gctggcgaag cgcgcaatgt gagcgcccgg cgtttatctc
     1921 ggtcggcgca ctgcaggcgc tggcacctga cgttatattt cgccggcttt gacgcggagg
     1981 agtaaatgaa aatagcggcc attgatcaca tcgtgttgcg gacctcgcgg ctggacgaca
     2041 tgctggattt ctactgccgg gtactgggct gtcaggtcga acgcgaaacc ggtcccgaaa
     2101 tcggcctggt gcagctgcgc gccggcaac
//
LOCUS       DATCZV010000166         4232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_330370, whole genome shotgun sequence.
ACCESSION   DATCZV010000166 DATCZV010000000
VERSION     DATCZV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330370"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VIW27_03645"
     CDS             complement(<1..390)
                     /locus_tag="VIW27_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738792.1"
                     /translation="MNWQTGECVAVKHRHRIRRQYDGGGMGAASPGSRLVRALVLVVT
                     FFGGLLCLVASLGRVFGSLFGSALGILRGLLGSLFGGALGIFSGFFGGLLGGSFSFFS
                     GFFGSLFGGALGVLRGLLGSLFGGALGI"
     gene            452..1711
                     /locus_tag="VIW27_03650"
     CDS             452..1711
                     /locus_tag="VIW27_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5738793.1"
                     /translation="MLYRLLLPLIVLLPLVLPALAASPAAETEFEAGTRAFKAEKYQQ
                     ALDHFERAEQAGMSEARLFYNLGAVHYRLDNYDRSRRYFERLLDHPQLGALGYYNLGL
                     IAHKSGDRADAIAYFEKCSEISADQGLAALAEKQIASLGGRPPKSWFGYVSAVYGYDS
                     NITLLTSSSAANQSGDFVQALALGELTLAGKPEDSYELTLLLLATDFLDGDEFDDDLL
                     TVGVERRRRAGDWGINYGLEAGTSTFAGEDYTDTLALSAGGRTGFEPDHELRLRLTLE
                     DITASSSDFDFVAGSRAEFNASYRIDSGSREYRFEYGLESNDRQNTETDSFSPTRHRL
                     RLRYFHQLGASTEIGASLEFRDSQYQGVPGDDRHDQRLRVRLESKYQFDAGWGAQSEI
                     TYTDNDSNQTGSSYHKYQALVSINAVF"
     gene            2336..3289
                     /locus_tag="VIW27_03655"
     CDS             2336..3289
                     /locus_tag="VIW27_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738794.1"
                     /translation="MPIIERDPWRQQYFDQVECPPELLIPTDDEHAYELYPEHRWIYN
                     KLLICDTQDYAGAPHGVPPPGFPVFSKPIYNMHGMGVGSRIIHNQQELDDNMTAGYMW
                     MVLADGEHVSSDVVVIGGEAQWWRHSVGKALGDGVFDHWTVQAAERPEIERYCGDWLR
                     RNLRGYSGAVNMETIGGHIIEVHLRFADQWPDLYGEGWLEAIVELYRRHRWQFADDDR
                     RDGYSVVLFGAHGVPYRHPPAALVEEIAARPGVSSVQITFHENLAPELHAMPPGGFRL
                     AIVNCWQLQAGLQAREDLALALWSTQALGKHRQPADVPAAE"
     gene            3298..>4232
                     /locus_tag="VIW27_03660"
     CDS             3298..>4232
                     /locus_tag="VIW27_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5738795.1"
                     /translation="MATRQRRSFPRPIEEIENLYIPLADGTRLAARLWLPTDARRDPV
                     PAILEYIPYRKRDFMRLRDEPIHRYYAGHGYAAIRVDLRGSGDSEGLLEDEYLPREQL
                     DALEVIDWLAAQPWCDGKVGMTGISWGGFNALQVAAHRPPALRAIITLCASDDRYADD
                     AHFMGGCLLNENQIWGTAFFANNALPPDPALAGDRWREMWLRRLEANRPFPALWLRHQ
                     RRDDYWKQGSVCEDFSRIGCAVYAVGGWADGYSNAVPRLLAGLECPRKGLIGPWAHTF
                     PHNGIPGPAIGYLQEALRWWDHWLKGSDDGIMDEP"
BASE COUNT          814 a         1405 c         1337 g          676 t
ORIGIN      
        1 aatgccgagc gcgccgccga acaggctgcc gagaaggccg cggagaacgc cgagcgcgcc
       61 gccgaacagg ctgccgaaaa agccgctgaa aaagctgaac gagccgccga gcaggccgcc
      121 gaaaaagccg ctgaaaatgc cgagcgcgcc gccgaacagg ctgccgagaa ggccgcggag
      181 aatgccgagc gcgctgccga acaggctgcc gaaaacgcgg ccgaggctcg cgacaaggca
      241 caacaggccg ccgaaaaagg taacaacaag aacaagcgcg cgaactaagc gggaaccagg
      301 gctcgccgcc cccattccac cgccatcata ctgccgacga atccggtgtc ggtgcttaac
      361 agcgacgcat tcgcctgtct gccagttcac ctgcccggca gcggccgcag gtaatgcata
      421 cgctttgcca taactctgta ataatcaatg aatgctttac cgtttgttgc taccgctgat
      481 cgtgctgctg ccgctcgtgt tgccggcgct ggcagcaagc cccgccgcgg aaaccgagtt
      541 cgaggcgggc acgcgcgcct tcaaggcgga aaagtatcaa caggctctcg atcacttcga
      601 gcgcgccgag caggctggca tgagcgaggc gcgcctgttc tataatctcg gcgcagtgca
      661 ttaccgcctc gataactacg accgcagccg ccgttacttc gaaaggctgc tggatcaccc
      721 gcaactcggc gcactgggct attacaatct cggcctgatc gcccacaaga gcggcgatcg
      781 cgccgacgcg atcgcctact tcgaaaaatg cagcgagatc agcgccgacc agggtctcgc
      841 ggcgctcgcc gaaaagcaga tcgcgtccct cggcggtcga ccgccgaagt cctggttcgg
      901 ttacgtctcc gcggtctatg gctacgactc caacattacg ctgctgacat cgagcagcgc
      961 cgccaaccaa tcgggtgact tcgtgcaggc actggcgctc ggcgaactga cgcttgccgg
     1021 caaacccgaa gacagttacg agctgaccct gctgttgctc gccaccgact ttctcgacgg
     1081 cgacgagttc gatgacgacc tgctcacggt cggcgtcgag cgccgccgtc gcgcgggtga
     1141 ctggggcatc aattacggcc tcgaggcggg aacctcgacc ttcgccggcg aagattacac
     1201 cgacaccctc gcgctgtccg cgggcgggcg caccgggttc gagcccgatc acgaattgcg
     1261 cctgcgcctg acgctggaag atattaccgc gagcagcagc gatttcgatt ttgtcgccgg
     1321 cagccgcgcc gagttcaacg cctcctaccg catcgacagc gggtcgcgcg agtatcgctt
     1381 cgaatacggc ctggaaagca acgaccgcca aaataccgaa accgatagct tttcaccgac
     1441 gcgtcaccgg ttgaggctgc gctacttcca ccagcttggc gccagcaccg agatcggcgc
     1501 cagtctcgaa ttccgtgaca gccaatacca gggagtaccc ggcgacgacc gtcacgatca
     1561 gcgcctgcgc gtgcgcctgg agagcaagta ccagttcgat gccggctggg gcgcacagtc
     1621 agagatcacc tataccgaca acgattccaa ccagaccggc tcgagctacc acaagtacca
     1681 ggcgctggtg tcgatcaacg cggtgttcta gcgcccggct gcctgccgtg ccaggtgcac
     1741 cttgacgtct tcgcaacccg aggcgttcaa attccatttt gagtagccag ccagtcggcc
     1801 cggctcgctt gccgacgatc ccggcctgcg ggcgtttgct ggcctgcaga tgcctgcaaa
     1861 ggtttacggc aaggcccggc tgcgcaagct gctgtcgccg ttccatgcgg tgcggccggc
     1921 gcgccgtgaa gttccacgcg cttatctttc gatgccgttg aaacggcgaa gaacgcgcgc
     1981 ctgccaccgc cgcggcggtg aaccggcgtc acgtaccagc tgccgccggc gacgaggcgc
     2041 atcgcccgcg cttcccggcg ccgattccga ttgccatttc gcggtgccgc attcgataat
     2101 atcgtgccgg gcaatagcgt ctaagcgcga cggcccggcg tatcggcaac atggcctgca
     2161 gtctttcgag gtcggcgtaa caggatcgtg aaaaatattc ggtttacgcg gcagccggag
     2221 cgaccggcga gaccacgagg acgaggactc gagccgatac agcgattgcc accggcggcc
     2281 gcaacgacag ttcgccgccg gggattgcag gttaccgcgc agctggagaa caacgatgcc
     2341 gatcattgaa cgcgacccct ggcgacagca atacttcgac caggtcgagt gcccgccgga
     2401 gctgctcatt ccgaccgatg acgagcatgc ctacgaactg tatcccgagc accgctggat
     2461 ctacaacaag ctgttgatct gcgacaccca ggactatgcc ggcgcgccgc atggcgtgcc
     2521 gccaccggga ttcccggtat tcagcaagcc gatctacaac atgcacggca tgggcgtcgg
     2581 cagccgcatc atccataacc agcaggaact cgacgataat atgaccgccg gctacatgtg
     2641 gatggtgctg gccgacggcg agcatgtcag cagcgacgtg gtggtcatcg gcggcgaggc
     2701 gcaatggtgg cgccatagcg tcggcaaggc gctcggtgac ggggtgttcg accactggac
     2761 cgtgcaggcc gccgagcggc ccgagatcga gcgatactgt ggcgactggc tgcgcaggaa
     2821 tctgcgcggc tacagcggcg cggtgaacat ggaaaccatc ggcgggcaca tcatcgaggt
     2881 acacctgcgc ttcgccgatc aatggcccga cctctatggc gagggctggc tcgaggccat
     2941 cgtcgaactc taccgccgcc atcgctggca attcgccgat gacgaccgcc gcgacggtta
     3001 cagcgtggtg ctgttcggcg ctcacggcgt gccctatcgc catccgccgg ccgcgctggt
     3061 cgaagaaatc gccgcgcgtc ccggggtttc cagcgtgcag atcacctttc acgaaaacct
     3121 ggcgcccgaa ctgcacgcga tgccgcccgg cggctttcgg ctggcgatcg tcaattgctg
     3181 gcaattgcag gccggtttgc aggcccgcga ggatctcgcg ctggcgctgt ggtcgaccca
     3241 ggcgctgggc aagcatcgac aaccggccga cgtaccggcc gcggagtagc acagcgcatg
     3301 gcaacccggc aacggcggag ttttccgcgt ccgatagaag agatcgagaa cctgtatatt
     3361 ccgctcgccg acggcacgcg actggcggcg cgcctgtggc tgccgacgga cgcgcgacgc
     3421 gacccggtgc cggcgatcct cgagtacatt ccctaccgca agcgcgattt catgcgcctg
     3481 cgcgacgagc cgatccatcg ctactacgcg ggccacggct atgccgcgat tcgcgtcgac
     3541 ctgcgcggca gcggcgactc ggagggcttg ctcgaggacg agtacctgcc gcgcgaacag
     3601 ctcgacgcgc tggaagtcat cgactggctc gccgcccagc cctggtgcga cggcaaggtc
     3661 ggcatgaccg gaatttcctg gggcggcttc aacgcgctgc aggtcgccgc gcatcgcccg
     3721 ccggcactgc gagcgatcat cacgctgtgc gccagcgacg accgctacgc cgacgatgcg
     3781 catttcatgg gcggttgcct gttgaacgaa aaccagatct gggggacggc atttttcgcc
     3841 aacaacgcgc tgccgcccga cccggcgctg gccggcgatc gctggcgcga aatgtggctg
     3901 cggcgcctcg aggccaaccg gccgttcccg gcgctgtggt tgcgccacca gcgccgcgac
     3961 gactactgga agcagggatc ggtgtgcgag gacttcagcc gcattggttg cgcggtgtac
     4021 gcggtcggcg gctgggccga tggctacagc aacgcggtac cacgcctgct ggcgggtctc
     4081 gaatgcccgc gcaagggcct gatcgggccg tgggcgcata cctttccgca caacggcata
     4141 cccggccccg cgatcggcta cctgcaggaa gccctgcgct ggtgggatca ctggctgaaa
     4201 ggcagcgacg acggcatcat ggacgaaccc at
//
LOCUS       DATCZV010000167         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_65154, whole genome shotgun sequence.
ACCESSION   DATCZV010000167 DATCZV010000000
VERSION     DATCZV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65154"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VIW27_03665"
     CDS             complement(<1..1377)
                     /locus_tag="VIW27_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019017639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY5738796.1"
                     /translation="MTVIKQNDLVDSVADALQYISYFHPPDFIRALDEAYQREQNPAA
                     RDAIAQILINSRMCAMGHRPICQDTGIVTVFVSVGMNVQWDAEMSVTEMCNEGIRRAY
                     NFPDNLLRASILEDPDGARRNTGDNTPGVIHYDIVPGDEVEVHVAAKGGGSEAKSKFA
                     MLNPSDSVVDWVLEQVPKMGAGWCPPGMLGIGVGGTAEKAMLLAKEALMEPIDIHDLQ
                     ARGASNRAEELRLELYDKVNRLGIGAQGLGGLTTVMDVKVRDFPSHAANKAVAIIPNC
                     AATRHAHFVLDGSGPARLPTPRLEDWPEVTRETGDNVRRVNLDKVTRDEVKTWKPGDT
                     LLLSGKMLTGRDAAHKRMVDMLSRGETLPVDLGGRFIYYVGPVDPIGDEVVGPAGPTT
                     ATRMDKFTRTMLEQTGLLGMIGKAERGPAAIDAIREFESVYLMAVGGAAYLVAQAIKA
                     AKVVAFEDL"
     gene            complement(1562..>2198)
                     /locus_tag="VIW27_03670"
     CDS             complement(1562..>2198)
                     /locus_tag="VIW27_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5738797.1"
                     /translation="LGCLPLQERDLQVAQRHKAWIAVGSFTLAIALATAGIIGLPAAL
                     ALVCIAMVAFNIVPLKEIYTSIEWPVIVLLGSMLPLGAALEATGGTALIARYIVEFAE
                     AYGAVVVLGLLMVATMTLSDVLDNAATTVVMAPIALDIANRLGVNPDSFLIGIAVAAS
                     CAFLTPIGHKNNTLIMGPGGYRFGDYWRMGLPLEIIVLVVSIPMILLVWPF"
BASE COUNT          409 a          709 c          672 g          408 t
ORIGIN      
        1 caggtcctcg aaggcgacga ctttcgccgc cttgatggcc tgcgcgacca ggtaggccgc
       61 accgcccacc gccatcaggt agaccgattc aaactcgcga atcgcgtcga tcgcggccgg
      121 cccgcgctcc gccttgccga tcattcccag caggccggtt tgctcgagca tggtgcgcgt
      181 gaacttgtcc atgcgcgtcg ccgtggtcgg cccggcgggc ccgactacct cgtcgccgat
      241 cgggtcgacc gggccgacgt agtaaatgaa cctgccgccg agatctaccg gcagggtctc
      301 gccgcgcgac agcatgtcga ccatgcgctt gtgcgcggcg tcacggccgg tcagcatctt
      361 gccggacagt aacagcgtat cgcccggctt ccaggtcttg acctcgtcgc gggtcacctt
      421 gtcgaggttg acgcggcgca cgttgtcgcc ggtctcgcgc gtgacctcgg gccagtcttc
      481 gagccgcggc gtcggcaacc gcgccggacc gctgccgtcg agcacgaagt gcgcgtggcg
      541 cgtcgccgcg caattgggaa tgatcgctac cgccttgttg gccgcgtgcg atggaaaatc
      601 gcgcaccttg acgtccatta ccgtggtcag cccgccgaga ccctgcgcgc cgataccgag
      661 acggttgacc ttgtcataga gttcgaggcg cagttcctcg gcacggttgc tggcgccgcg
      721 cgcctgcagg tcgtgtatat cgatcggctc catcagcgct tccttggcca gcagcatcgc
      781 cttttcggcg gtgccgccga cgccgatgcc gagcatgccg ggcggacacc agccggcgcc
      841 catcttcggc acctgctcga gcacccagtc gaccaccgag tccgacgggt tgagcatcgc
      901 gaacttcgac ttggcttcgc tgccgccgcc cttggccgcg acgtgtacct cgacttcatc
      961 gccgggcacg atatcgtagt ggatcacgcc tggagtattg tcgccggtat tcctgcgcgc
     1021 accgtcggga tcttcgagga tcgacgcgcg cagcaggtta tccgggaagt tgtaggcgcg
     1081 gcggatgccc tcgttgcaca tctcggtgac cgacatctcg gcgtcccatt gcacattcat
     1141 gccgacgctg acaaataccg tgacgatgcc ggtatcctgg cagatcggcc ggtgacccat
     1201 cgcgcacatg cgcgaattga tcaggatttg cgcgatcgcg tcgcgcgccg ccggattctg
     1261 ttcgcgctgg taagcctcgt ccagcgcgcg aatgaaatcc ggcggatgaa aataagagat
     1321 atattgcagt gcgtcggcga cgctatcgac caggtcgttt tgcttgatca cggtcatgaa
     1381 atcggttccc ggcttcggca agtttcgcca atgataccgc ctttaataat tggggtcaga
     1441 ttcaaattag cgcattcacc ccctaaaatg gggtcagagt catttttttc gaatctaatt
     1501 gaagtcagta aaccggggga acctcagagt caattttcta aaaaatgact ctgaccccaa
     1561 tttagaaggg ccagacgagg aggatcatcg ggatggagac gacgaggacg atgatctcga
     1621 gcggcaggcc catacgccag tagtcgccga aacggtagcc gcccgggccc atgatcagcg
     1681 tattgttctt gtgcccgatc ggggtcagga aagcgcagga ggccgccacc gcaattccga
     1741 tcaggaacga atcagggttg accccgagcc ggttcgcgat atcgagggcg attggcgcca
     1801 tgaccaccgt ggtcgcagca ttgtcgagca catcggacag cgtcatggtc gccaccatca
     1861 acaggccgag cacgaccacg gcgccgtagg cttcggcgaa ctcgacgata taccgcgcga
     1921 tcaatgcggt cccgccggtg gcttccagcg cggcacccag cggcaacatc gagcccagca
     1981 gcacgatcac cggccactcg atcgacgtat agatctcctt cagcggcacg atgttgaagg
     2041 cgaccatcgc aatgcaaacc agcgctagcg ccgcgggcag gccgattatg ccggcggtag
     2101 ccagcgcgat cgccagggta aaactgccga ccgcaatcca cgccttgtgc cgttgcgcca
     2161 cctgcaggtc gcgctcctgc agcggcaggc agccgagc
//
LOCUS       DATCZV010000168         7692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_383146, whole genome shotgun sequence.
ACCESSION   DATCZV010000168 DATCZV010000000
VERSION     DATCZV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383146"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1079
                     /locus_tag="VIW27_03675"
     CDS             114..1079
                     /locus_tag="VIW27_03675"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY5738798.1"
                     /translation="MVGKVASNDLMVFTGNANLELAKRIVGHLEMPLGIASVGTFSDG
                     EISVEIGENVRGKDVFIVQPTCAPTNDHLMELLVMIDAIKRASSNRVTAVIPYFGYAR
                     QDRRVRSQRVPITAKVVANMLTSVGVDRVLTVDLHADQIQGFFECPVDNVYASPILMG
                     DIWKQKYPQMIVVSPDVGGVVRARAIAKQLDEAELAIIDKRRPQPNMSKVMNIIGDVR
                     DKTCIIVDDMVDTAGTLCEAAGALKDKGAHGVYAYCTHAVLSGKAIENVQNSTMDELV
                     VTDTIPLSAEGAACTRIRQVSVAGMLAETIRRIHYEESVSSLYMD"
     gene            1172..1903
                     /locus_tag="VIW27_03680"
     CDS             1172..1903
                     /locus_tag="VIW27_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY5738799.1"
                     /translation="MSETIHLNAELRSDSGKGASRRLRRQGLVPAIVYGGDREPAKIS
                     IAHNEFIHELENETIYTQVIELRVADKRQDVILRDLQRHPYKNKVIHADFYRIDENKP
                     IKVVVPIHVANADKCVGVKGDGGVMTQMVTEIEIISLPKDLPEYLEIDVIDLHLGDIL
                     HLTDIQLPEGVQIVALTHLEDVEDIEHDDHNVGVISVFKPREEVIEEEAPEAPEAPEI
                     EGEEAGAEDQESGGEAASGEPRDEE"
     gene            1912..2496
                     /gene="pth"
                     /locus_tag="VIW27_03685"
     CDS             1912..2496
                     /gene="pth"
                     /locus_tag="VIW27_03685"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY5738800.1"
                     /translation="MPALTADIRMIVGLGNPGADYVDTRHNAGFWMIDLLAADCGASF
                     RFDKRYNAEVGKLRADGRDVHLQKPLAFMNRSGQSVAGLARYYKLEPSQVLIMHDELD
                     LPPGTNRIKLDGGHGGHNGLRDVIAHLGSREFFRLRIGIGHPGDSRAVTNYVLRRPSV
                     ADRGAIEAANRDTLAVMPLLLEGRVDKAMQALHT"
     gene            2507..3598
                     /gene="ychF"
                     /locus_tag="VIW27_03690"
     CDS             2507..3598
                     /gene="ychF"
                     /locus_tag="VIW27_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY5738801.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNSLTRAGIAAENYPFCTIDPNVGVV
                     EVPDPRLQKLADIVQPKKIVPTTMEFVDIAGLVAGASKGEGLGNQFLGHIRETDAIAH
                     VVRCFEDDDIAHVGNAVDPLADIETINTELLLADMESVARQAERAAKQAKSHDKEAMA
                     RHEFMQAVQAHVEAGKPARSLELDARRSPWLKELHLITAKPVLYIANVAEDGFEDNPH
                     LDAVREFAVGEGAEVVAVCAAIEADIAELDVAEAAEFLDEMGLDEPGLDRVIHAGYRL
                     LQLQTFFTAGPQEVRAWTVRAGATAPRAAGRIHTDFEKGFIRAEVVAYDDFVACNGEQ
                     GAKDAGKWRLEGKDYVMREGDVVHFRFNV"
     gene            3697..5001
                     /locus_tag="VIW27_03695"
     CDS             3697..5001
                     /locus_tag="VIW27_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEY5738802.1"
                     /translation="MTNPAIPGAEFLPLDEIRLLQRERWARQREYLQARSAFYGNNFA
                     PLPRSLDEFVDFPLTDKQMLRDDQARHPPFGSYLASDADRISRVHRTSGTTGVAMNLA
                     LSRADAEMTASVAGRAQSAAGLGPGYRVVHCLNYCLWMGGYSDHAGLEATGATVVPYG
                     VGGSEGLIRVIQELGIDAISCTPSYPGLLEQVIADKFPGLEPRDLGLRLGLFGGEPGL
                     DEAAFRERLESTWGFEARNANFGVSDVLCNFAAQTEQCSDLHLVGCDVLYPELIEPQS
                     GDLLPWREGERGELVLTHLAKECQPLLRFRSGDIVDLTATDIAACGRSAPRFRVVGRS
                     DDMVVVRGLNLFPAMIGGVLHRFAELSGGWQVRLDAPPPYDFLPLQAELAYPVGEGEA
                     LARRVEAAIKAELGASARVVLLDPGSLPRTEGKTRYVFRTCQ"
     gene            4998..5756
                     /locus_tag="VIW27_03700"
     CDS             4998..5756
                     /locus_tag="VIW27_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5738803.1"
                     /translation="MSEFEYRTVRSECVDGIRTITLNRPQSLNAMNRALIDDVAQAFD
                     DANRDPQTRVIVLTGAGRAFCAGDDRKAHVHPRNETEARELVDAIQRATRSMLLGDKP
                     VLGAINGWAVGGGFEWALNCDLSIWARGARAFFPEVSLNLFVTGAVTAILPAMVGLVK
                     AREMLYLGRRYDAAELQRLGVAWRVVDDEHLVETCHEAAAELAALPPKSLAAMKRVLN
                     QCAAADIERALELETEATVAGFLDPETTRRLKAF"
     gene            5856..5932
                     /locus_tag="VIW27_03705"
     tRNA            5856..5932
                     /locus_tag="VIW27_03705"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5890..5892,aa:Met,seq:cat)
     gene            complement(5958..7043)
                     /locus_tag="VIW27_03710"
     CDS             complement(5958..7043)
                     /locus_tag="VIW27_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY5738804.1"
                     /translation="MAKKKTPGLVRRGRIWHFDKTIDGRRIQGSTGTRCLDEAEAVLS
                     HILTTHHRAKFYGIRPRRLFREAATKYLQEETKKSLHNDAQALRALDTFIGDLYLEQV
                     HSGTLEPFVRHMRDKGRSAGTINRYIAVVRRILNLAARYWRDVDSGLTWLETAPLFRL
                     EKGPRRNPYPISWDEQRLLLSELTPTLARMALFLINTGVRDQELCSLRWSWQQAEDLF
                     ILPGHCSKNGQERFVVLNGIAQSVVREQRGKDDQRVFPRRRVYSKGWKHGRERAANRY
                     EDELGAPCPWGFRHLRIHDLRHTFGRRVRAAGWSNEDRKDLLGHKNGDITTHYSRVEI
                     DHLREVVASITRPISYNSPTLRAVGNV"
     gene            7436..7687
                     /locus_tag="VIW27_03715"
     CDS             7436..7687
                     /locus_tag="VIW27_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY5738805.1"
                     /translation="MQDLVNSGATSLAQQITERYGLLLSQAQLAELLGRSADGLRYSL
                     KCPSDSSTRELKACGRKVGRRIYYPSSEVARILLGSGEA"
BASE COUNT         1457 a         2377 c         2441 g         1417 t
ORIGIN      
        1 attcgttggt tcgaatccag ccggcccagc caaacaacgg ggctttcgcc gggttcccgc
       61 gaggcgcgga aaccggcgag ggccctcaaa gtttatgtaa gcggaagagc tcgatggttg
      121 gcaaagtagc ctcgaacgat ctgatggtgt ttaccggtaa cgccaacctg gaactggcca
      181 aaaggatagt cgggcacctg gaaatgccgt tgggcattgc atcggtcggg actttcagcg
      241 acggcgagat ttccgtggag atcggcgaga acgtgcgcgg caaggatgtt ttcatcgtcc
      301 agccgacctg cgcgccgacc aacgatcacc tgatggaatt actggtgatg atcgacgcca
      361 tcaagcgtgc ctcgtcgaac cgggtgacgg cggtgatacc gtatttcggt tatgcgcggc
      421 aggaccgtcg agtgcgctcg cagcgggtgc cgatcaccgc caaggtggtc gccaacatgc
      481 tgaccagcgt cggcgtcgat cgcgtgctga ccgtcgatct gcatgccgac cagattcagg
      541 gctttttcga atgcccggtg gacaatgtct acgcgtcgcc gatcctgatg ggtgatatct
      601 ggaaacagaa gtacccgcag atgatcgtcg tctcgccgga cgtcggtggc gtggtgcgcg
      661 cgcgggccat cgccaagcag ctcgacgagg cggagctggc gatcatcgac aagcggcggc
      721 cgcagccgaa catgtcgaag gtgatgaata tcatcggcga cgtcagggac aagacctgca
      781 tcatcgtcga tgacatggtc gacaccgccg gtacgctgtg cgaagcggcc ggggcgctca
      841 aggacaaggg tgcacatggc gtttatgcct attgcacgca cgcggtattg tcgggtaagg
      901 caatcgagaa cgtgcagaat tcgaccatgg acgaactggt ggtcacggat actatcccgc
      961 tgtccgcgga gggcgcggcc tgtacccgaa ttcgccaggt gtcggtggcg gggatgctcg
     1021 cggaaaccat tcgccgcatc cactacgaag aatcggtcag ttcgttgtat atggactgac
     1081 gatcgccgcg caagcgcggc gcaacgaaac ctgtctggtc gcaaggcagg cctccgtcat
     1141 ttggcggatt tttatttttt ggagtacagc aatgtcagaa acgatacatt tgaatgctga
     1201 actgcgctct gactcgggga aaggtgcgag ccgccgcctg agacgccagg gcctggtacc
     1261 agcaatcgtc tacggcgggg atcgcgaacc cgcgaaaatc agcatcgcgc acaacgagtt
     1321 cattcacgag ctcgaaaacg agaccattta cacccaggtc atcgaactgc gtgtcgccga
     1381 caagaggcag gacgttattc tgcgtgacct gcaacgccac ccgtacaaaa acaaggtcat
     1441 ccacgcggat ttttaccgca tcgacgaaaa caagccgatc aaggtcgtgg tgccgatcca
     1501 tgtcgccaat gccgataagt gtgtcggcgt caagggcgac ggcggcgtca tgacgcaaat
     1561 ggtgaccgag atcgagatca tctcgctgcc gaaggatctg cccgagtacc tggaaatcga
     1621 tgtgatcgac ctgcatctcg gagacattct tcacctgacc gacatccagc tgcctgaagg
     1681 cgtgcaaatc gtcgcgctga cgcacctcga ggacgtcgag gatatcgagc acgacgacca
     1741 caatgtcggc gtgatctcgg tattcaagcc gcgcgaggag gtcatcgaag aagaggcacc
     1801 ggaagctccg gaagcgcccg agatcgaagg cgaggaagcc ggcgccgagg accaggagtc
     1861 cggtggcgaa gctgccagcg gcgagccgcg cgacgaggag taatcacccg cttgcctgcg
     1921 ctgaccgcag atattcgtat gatcgtcggc ctcggtaacc ccggggccga ttacgttgat
     1981 acgcggcaca atgccggctt ctggatgatc gatctgctgg ccgccgattg cggggcgagt
     2041 tttcgtttcg acaagcgcta caacgccgag gtcggcaagc tgcgcgctga cggccgcgac
     2101 gtacacctgc agaaaccgct ggcgttcatg aaccgcagtg gccagtcggt agccgggctg
     2161 gcgcgctatt acaagctcga accgtcgcag gtcctgatca tgcatgatga actcgacctg
     2221 ccgccaggca ccaatcgcat caagctcgac ggcgggcacg gcgggcacaa cggactgcgc
     2281 gatgtgatcg cccatctcgg cagccgcgaa ttctttcgcc tgcgcatcgg tatcggccat
     2341 cccggtgaca gccgcgcggt caccaactac gtgctgcgca ggccgtcggt cgccgatcgc
     2401 ggggccatcg aggctgccaa ccgcgatacg ctggcggtca tgccattgtt actggaaggg
     2461 cgcgtcgaca aggcgatgca agccctgcac acctgaggaa atcgcgatgg gattcaagtg
     2521 cggtatcgtc ggtctgccga atgtcggcaa gtcgacgctg ttcaactcgt tgacccgcgc
     2581 cggcatcgcc gccgagaact acccgttctg taccatcgac ccgaacgtcg gcgtggtcga
     2641 ggtccccgat ccgcgcctgc aaaagctcgc cgacatcgtg cagccgaaga aaatcgtgcc
     2701 gacgacgatg gagttcgtcg atatcgcggg actggtcgct ggcgcgtcga agggcgaggg
     2761 tctcggcaac cagtttctcg gacacattcg cgagaccgac gccatcgcac acgtggtgcg
     2821 ctgcttcgag gacgacgaca tcgcccatgt cggcaacgcg gtcgatccgc ttgccgacat
     2881 cgagaccatc aacaccgagc tgctgcttgc cgacatggaa tcggtcgccc ggcaggccga
     2941 gcgtgccgca aagcaggcca agtcgcacga caaggaagcc atggctcggc acgaattcat
     3001 gcaggcggtg caggcacacg tcgaagccgg caagcccgcg cgcagcctcg agctcgacgc
     3061 tcgcagaagc ccgtggctga aggagctgca tctgattacc gccaagccgg tactctacat
     3121 cgccaatgtc gccgaggacg gtttcgagga caacccgcat ctcgacgcgg tgcgcgagtt
     3181 cgccgtcggc gagggcgccg aggtagtcgc ggtctgcgcc gcgatcgaag cggacatcgc
     3241 cgaactcgat gtcgccgagg cggccgagtt cctcgacgag atgggtctcg acgagccggg
     3301 gctcgaccgc gtgatccatg ccggataccg cttgctgcaa ttacagactt tctttaccgc
     3361 cggcccgcag gaagtgcggg cctggacggt gcgcgccgga gctaccgcgc cgcgcgcggc
     3421 tgggcgcatc cataccgact tcgaaaaggg cttcattcgc gccgaagtcg tcgcctacga
     3481 tgatttcgtc gcctgcaacg gcgagcaggg ggccaaggac gccggcaagt ggcgcctcga
     3541 gggcaaggat tacgtgatgc gcgagggcga cgtcgtgcac ttccgcttca acgtctgatt
     3601 gggggttgca ggcgccggcg cgctggtgcg ctcggccgct gctggttata ctgcgcctgt
     3661 tctgcgacgg agcagtcgtg attattctcc aggggcatga ccaatcccgc gattcccggt
     3721 gccgagttcc tgccgctcga cgaaattcgc ctgctgcagc gcgagcgctg ggctcggcag
     3781 cgcgaatacc tgcaggcccg ctcggccttc tacgggaata atttcgcccc cctgccgcgc
     3841 tcgctcgacg agttcgtgga cttcccgctc accgacaagc agatgctgcg cgatgaccag
     3901 gcccggcacc cgccgttcgg cagttacctc gccagcgacg cagaccggat cagccgggtc
     3961 caccgcacct ccggcaccac cggcgtggcg atgaacctcg cgctcagccg cgccgacgct
     4021 gaaatgaccg cgagcgtcgc cggcagggcg caatccgcgg ccggactggg ccccggctac
     4081 cgggtggtgc attgcctcaa ctactgtctg tggatgggcg gctatagcga ccatgccggg
     4141 ctcgaggcga ccggcgcgac ggtggtcccc tacggtgtcg gcggctcgga aggcctgatc
     4201 agagtgatcc aagaactcgg catcgacgcg atttcctgca cgccgtccta ccccggactg
     4261 ctggaacagg tgatcgccga taagtttccc ggcctcgaac cacgcgacct cggcctgcgg
     4321 ctcggcctgt tcggcggcga accgggactc gacgaagcgg cgtttcgcga acgcctcgaa
     4381 tcgacctggg gcttcgaggc gcgcaacgcc aatttcggcg tctccgacgt gctgtgcaat
     4441 ttcgccgcgc agaccgagca gtgctcggat ttgcacctgg tcggctgcga tgtgctctat
     4501 cccgagctga tcgaaccgca atccggtgac ctgctaccgt ggcgcgaagg cgaacgcggc
     4561 gagctggtac tgacccacct tgccaaggaa tgccagcctt tgctgcgttt tcgcagtggc
     4621 gatatcgtcg atttgacggc caccgatatc gccgcctgcg ggcgcagtgc accgcgtttt
     4681 cgcgtcgtcg gtcgcagcga cgacatggtc gtggtgcgcg ggctcaacct gttcccggcg
     4741 atgatcggcg gcgtgctgca tcgtttcgcc gagttgtccg gcggctggca ggtgcgactc
     4801 gacgcgccgc caccgtacga tttcctgccg ctgcaggccg aactcgcgta tccggtcggc
     4861 gaaggcgagg cgctcgcgcg ccgcgtcgag gcggccatca aggccgagct cggcgccagc
     4921 gcgcgggtcg tgctgctgga tcccggcagc ctgccgcgaa cggagggcaa gacgcgttac
     4981 gtcttcagga cctgccaatg agcgaattcg agtatcgaac cgtgcgcagc gaatgcgtcg
     5041 acggcatcag gacgattacc ctgaatcggc cccagagcct gaacgcgatg aaccgcgcgc
     5101 tgatcgacga tgttgcacaa gcctttgacg atgccaaccg tgatccgcag acacgcgtca
     5161 tcgtgcttac cggcgccggg cgcgcgtttt gcgccggcga cgatcgcaag gcacacgtgc
     5221 atccgcgaaa cgagaccgag gcgcgcgaac tggtcgacgc gatccagcgg gccacgcgct
     5281 cgatgctgct cggcgacaaa ccggtgctcg gcgcgatcaa cggctgggcg gtcggcggcg
     5341 gcttcgaatg ggcgctcaac tgcgacctgt cgatctgggc gcgcggcgcg cgcgcctttt
     5401 tccccgaggt gtcgctgaac ctgttcgtga ccggcgcggt gaccgcgatc ctgccggcga
     5461 tggtggggct ggtgaaggca cgcgaaatgc tttacctggg ccggcgctac gatgccgcgg
     5521 aactgcagcg cctcggggtg gcctggcgag tggtcgatga cgagcatctc gtcgagacct
     5581 gccacgaagc ggcggccgaa ctggcggctt tgccgccgaa atccctggcc gcgatgaaac
     5641 gcgtgctgaa ccagtgtgcc gcggccgata tcgagcgcgc cctcgaactc gagaccgagg
     5701 ccaccgtggc cggcttcctc gaccccgaga ccacccgccg cctgaaggcg ttttgagcat
     5761 ggccgcacct cggcttggcg cttgacacta cccgccgatc acgcgaaaat cgcgcgctct
     5821 ccaaggctgg gcaacccagc cggcagaatt tccatggcta cgtagctcag ctggttagag
     5881 cacatcactc ataatgatgg ggtcccctgt tcaaatcagg gcgtagccac catttttttc
     5941 aatgacttag gctccaatta cacgtttccg accgccctca atgtaggaga attgtaggag
     6001 attggcctgg tgatcgatgc cacgacctca cgtaaatggt cgatttcgac ccggctgtag
     6061 tgagtcgtga tgtcgccatt cttgtgtccc aacaagtctt ttcggtcttc gttgctccag
     6121 cccgccgcgc gcacccggcg cccgaaggta tgccgcagat catggatccg caggtggcga
     6181 aagccccagg ggcagggtgc gccgagctcg tcctcgtaac gattcgccgc gcgttcccgt
     6241 ccgtgcttcc agcccttgct gtagacccgt ctgcgcggga acacgcgctg atcgtccttg
     6301 ccgcgttgct cccgtactac cgactgcgcg atcccgttca acaccacgaa tcgttcctgg
     6361 ccattcttcg agcagtgccc gggcagaatg aacaggtcct cggcctgctg ccagctccag
     6421 cgcagcgagc agagttcctg gtcccgcacc cctgtgttga tcaggaacaa cgccatccgc
     6481 gcaagcgtcg gggtcagttc cgacaacagc aggcgctgct cgtcccagct gatcggatag
     6541 ggatttctcc gcgggccttt ctccaggcgg aataacggtg ccgtttcgag ccaggtcaga
     6601 ccgctgtcga catcgcgcca gtaacgcgcg gccaggttca ggattcgccg caccaccgcg
     6661 atgtagcggt tgatggtgcc ggcgctccga cccttgtcgc gcatgtgccg cacgaagggc
     6721 tccagcgtcc cgctgtgcac ctgctcgaga tacagatcgc cgataaaggt atccagcgcg
     6781 cgcagggcct gggcgtcatt gtgcaacgat tttttcgttt cctcctgcag gtacttggtt
     6841 gccgcttccc ggaacaggcg ccgtgggcga atgccgtaga acttggcccg gtgatgcgtg
     6901 gttaaaatat gactcaggac cgcttccgct tcgtcgaggc agcgcgtgcc agtgctgccc
     6961 tgaattcggc gtccgtcgat cgttttgtcg aagtgccaga ttcggcctcg tcgaacgagg
     7021 ccgggtgtct ttttctttgc catgagattt ctccacctgg cttagccgga cacccgttgc
     7081 gggagatata ttgatccacc caggcgtcca gttcaagtcg gtcgaaggcg atgccctgtt
     7141 taccaatgcg aatttcagtc aaatacggtc gcactatggc gttgaaacgg ttgcgatcca
     7201 tgccgagata gcgcggcgca tcgcgaaagc ggagcagtct cggtgtgatg ggtggcgagt
     7261 gttgcatttg tcgggaccgt aaatcgggta tctacaaatg gctcatggta gcgccgaaac
     7321 gggacgaaaa aacgctcgaa agctcgcact tggttggggc ttttcaagct ttttttcgtg
     7381 cacttagagg tggcaaactg gtggtttggc aaacgaaaaa tggagatcac cgatcatgca
     7441 agatctggta aattccggtg caacgtcgct ggcgcagcaa atcaccgaac gctacggcct
     7501 gttgctttca caggcccagt tggcggaact gctgggccga tcggccgatg gcctgcgtta
     7561 cagcctcaag tgcccgagcg actcgagcac acgagagctg aaggcctgcg gccgtaaagt
     7621 cgggcgccgg atctactacc catcctccga ggttgctcgg attctgctcg gtagcggcga
     7681 ggcatgagca at
//
LOCUS       DATCZV010000169        13284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_648081, whole genome shotgun sequence.
ACCESSION   DATCZV010000169 DATCZV010000000
VERSION     DATCZV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648081"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VIW27_03720"
     CDS             <1..269
                     /locus_tag="VIW27_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738806.1"
                     /translation="LQMVEHGIGIALVDHISAREHLGQRVAFRRFDSPISMKVWLMRP
                     RLRPRSMVVDQFIELLHASVDAGELDSPFAGNTPRGTGRGATVS"
     gene            complement(324..1103)
                     /locus_tag="VIW27_03725"
     CDS             complement(324..1103)
                     /locus_tag="VIW27_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738807.1"
                     /translation="MGEAIVQFLEAWSPLDVVLIAQNFDRFLMGAWVTLQLTVISLVI
                     GGLLSIPLAIVRAGSNPILNAPVWAFTYMFRGTPLLVQTYLIYYGLGQFEWIRESWLW
                     GPVLSQAWYCALIAFSLNTAAYTTELLRGAIVTTNKGEIEAAKACGLSRWMRMRRIIM
                     PSAFRRALPAYSNEVIFMLHGSVVASTITIQDLLGVGRWLNGRYYLAYEGFITAAVLY
                     LVIVLLISRGFRLWEKHWLAHLYPQDAVKAKPKKGFAAFRI"
     gene            complement(1096..1788)
                     /locus_tag="VIW27_03730"
     CDS             complement(1096..1788)
                     /locus_tag="VIW27_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738808.1"
                     /translation="MIIEYQDQLLAGTWVTIRLAVLSLVIAVLCGLLGAWAKLSKNIL
                     AQKTAAGYTTVVRGVPDLVLILLVFYGGQEFANQIGAVTGLWDYVEISQFAAGVGTIG
                     VVFGGYMTETFRGAILAIPRGQIEAGIACGMSRLTIFRRIIWPQMVRYALPGFSNNWL
                     VQIKATALVSVVGLQDVVYNGFVAGRSTRQLFTFMFAVLMIYLVLTAISDAGLRLLDK
                     KYSKGIARADDG"
     gene            complement(1860..2633)
                     /locus_tag="VIW27_03735"
     CDS             complement(1860..2633)
                     /locus_tag="VIW27_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020327769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5738809.1"
                     /translation="MKIKQLITTLGIALLLLGGASAQAADLRVGVEGAYPPFSWKESD
                     GTLKGFDIDIAEAICAEMGRNCVLVEQDWDGMIPALLAKKFDAIIASMSITEERKKRV
                     DFTDKYYNTPAKFVGKKGADIEISKGGLAGKRVGLQRGTTHQCFMEKMYPGVELVLYG
                     TQDEVFQDLAIGRIDLQFSDSIAADEGFLQTDAGKGFAFIGGDQHDEECHGPGAGIAV
                     RKSDGALRDELDAAIKAIRDKGIYQKINAKYFDFDIFGG"
     gene            complement(2711..3496)
                     /locus_tag="VIW27_03740"
     CDS             complement(2711..3496)
                     /locus_tag="VIW27_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010674267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5738810.1"
                     /translation="MSDATGGHAALEVVDLHKSFGEHEVIRGVSMRAHKGDVISILGA
                     SGSGKSTFLRCINLLEIPSAGEVFVAGEKIRMHHTKSGAYEPEDRRQVERMRARLGMV
                     FQSFNLWSHMTVLENVIEAPVHVLKLPKAEAIEEAEALLHKVGIHDRKDYYPAQLSGG
                     QQQRAAIARGLAMRPAVMLFDEPTSALDPELVGEVLKVMRDLAEEGRTMLVVTHEMGF
                     ARDVSSEVVFLHEGQIEEQGDPQRVFRNPDSERFRQFLSRTMQ"
     gene            complement(3607..4482)
                     /locus_tag="VIW27_03745"
     CDS             complement(3607..4482)
                     /locus_tag="VIW27_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY5738811.1"
                     /translation="MDWQVLAAMVARDRGLALREVQATPVGGGDINRAYRLRIDNRQF
                     FVKLNREMLLSMFEAEQAGLAAIAATGAIRVPAVVASGCLSGFAYIVMEYIDFGGRAD
                     AARLAAALAAMHGNCHARFGFAIDNTIGTTPQPNGFLDDWIEFWRKRRLEFQLQLAAA
                     NGIDSRVIDAGYRLAADLEPLFGDYQPRASLLHGDLWSGNQAADQAGNPVIFDPACYY
                     GDHEADLAMMELFGSPGRRFFEQYNDHFPIDSGYARRRDLYNLYHVLNHANLFGGGYA
                     ARAGHMIDGLLASLG"
     gene            complement(4482..5018)
                     /locus_tag="VIW27_03750"
     CDS             complement(4482..5018)
                     /locus_tag="VIW27_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5738812.1"
                     /translation="MSRSLLFNKPFRVLSQFSADGDKRTLAEFIDLPGVYAAGRLDYD
                     SEGLMLLTDDGALQARISDPRYKTAKTYLAQVEGRPSAATLADLARGVELKDGTTRPA
                     RVAAVDEPGWLWPREPPIRVRRHIPTQWLRLEIKEGRNRQVRRMTAAVGHPTLRLVRI
                     AIGDWTLDGLAPGEYRLL"
     gene            complement(5020..5937)
                     /locus_tag="VIW27_03755"
     CDS             complement(5020..5937)
                     /locus_tag="VIW27_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY5738813.1"
                     /translation="MLKLQFMRLFLVDSSIYVFRAWFGPDPERSDLDGRPNQAFVGFS
                     DFVYRLLTEQAPSRLVFAFDESLGTSARREIYPDYKANRAPAPVELKRQFGCCREWLE
                     ALGISCVSSPDWEADDLIGSLARYHRRQELPVAILTADKDLAQLITEGDLWWSYADNR
                     QLDYRAVCKKFGVRPEQIAAQLALAGDKVDNIPGIPEIGPRTAARLLKKYQTLDNLRR
                     HLHEVGEMKFRYAARVQQSLIDNQAQLDVGIRLTRINCELAEMARVETARGEPDAAIL
                     ERIMQEQDFGHARQARWQRWLESAAAERV"
     gene            6030..6182
                     /locus_tag="VIW27_03760"
     CDS             6030..6182
                     /locus_tag="VIW27_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738814.1"
                     /translation="MPSCTGCRRSAAAPINDARELAAYVVDLLEGFGPCEARPMFGGQ
                     GFFTRV"
     gene            complement(6236..7393)
                     /locus_tag="VIW27_03765"
     CDS             complement(6236..7393)
                     /locus_tag="VIW27_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY5738815.1"
                     /translation="MNQPYPSERPGTLLVPQGEFVLQRNPRDQRLQAWDAADEYLLGY
                     LDECRVPSRHGKLLLLNDAFGALAVALADHPLYSWNDSCLAQRALADNLVANGYTATQ
                     VATNSGIDLPAMAVDCVLIKIPRSLALLEHQLYCLRDVLHHDTLILGAGMARHIHRST
                     LELFETILGPATTTRARKKSRLIRVERDHSLQEGRSPYPDSYELFVDRSYQISNHASL
                     FSRDRLDPGSRLMIERMPIDPGLRRIVDLGCGNGVLGIVAAALNPPASLVFADESHMA
                     IASARDNFHRAFGRLREAEFRLGDCLEGLADGSADLVLVNPPFHQHHSVGDRVARNMF
                     RDAHRVLAAGGELRVIGNRHLGYHLRLGRLFGNCTTVAANSKYVLLSATRP"
     gene            complement(7390..8217)
                     /locus_tag="VIW27_03770"
     CDS             complement(7390..8217)
                     /locus_tag="VIW27_03770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY5738816.1"
                     /translation="MRVMEQAMETFAIDNEAGIRLAVFIAVFLVMGLWEWLSPRRRLT
                     VSKPLRWVNNLGLLAINSVVLRLLFPAAAVGLAYSAADLQWGLFNRVALPYWLEVVAA
                     VLLLDLSIYLQHLLMHRVPLLWRLHRVHHADLDIDLTTGSRFHSIEILLSMLFKWGVI
                     LLLGPALLAVLIFEVLLNGMALFNHANVRLPALADRVLRRLLVTPDMHRVHHSTRVTE
                     TNSNYGFNLSVWDRIFGTYIDQPQLGHDGMSIGLNEFRDPRRVDRLPGMLALPFTRP"
     gene            8473..10431
                     /locus_tag="VIW27_03775"
     CDS             8473..10431
                     /locus_tag="VIW27_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY5738817.1"
                     /translation="MLNDILLLLILSAVSVAIFRRIDVPAVLGYLVVGLLAGDRALGL
                     IHDSHAIEQIAEIGVVFLLFAIGLEVSIPRLLAMRNIVFGIGLTQVVISTLSTLVIGM
                     AFGLSWQAAFAVGGALAMSSTAVVTKLLTEQYELHQPHGNISLGVLLFQDLAVVPFLV
                     LIPILAAPGEGSLLVPISLALAKGALAFAVIFYAGQYLLRPAVRKVAATHSSELFTLF
                     VLSTALIAAWLTWQLGLSLAMGAFLAGIMLAETEYQHRIENEIRPFRDVLMGLFFISV
                     GSQFDWQVIINEPFEVAVLTVGLVFGKGFAITVISALAGYARDIAIRAGILLGQGSEF
                     GFAVLAVAITGGLLGLEPSQPIIAAIILSMVISPMMIRHNAAIAQRIAPAAENLPNPS
                     ETALEEACRDLQDHVLLCGFGRTGQNLGQFLRHQGIPFIALEIEADIVNEAREAGEPV
                     FIADSGNPRILKLAGIDKARVLVISFTEVEPAENICRQVRELRPDIPLIIRTRDDRHL
                     EHLLECGADEVIPDTVESSIMLARHTLTALGESGPDMDHLLEQARAGHYARIRAFFHS
                     VRDVNLGAADRHHLHSVEILGTYYAVGRSIDTMERLQQVRIITIRRNGVSSEDPPPEL
                     ELKTGDVLVIEGRPADIRAAEIQLMSGL"
     gene            10516..11169
                     /locus_tag="VIW27_03780"
     CDS             10516..11169
                     /locus_tag="VIW27_03780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY5738818.1"
                     /translation="MRIRPFGGLEVVIPPRFPRAQIAALVERHATWARRQLDRQARLR
                     DAIRLPQHLDLAYDNSSTPVIYATEPLTWTGDLFAEPSQAPIVVEAADQAARIGELRG
                     WIRRRAHQLLPPKLQALSRRTGLEYAGVGIRSQKTRWGSCSSRGHISLNDQLLFLPAD
                     TVDYLMIHELCHTRHLDHSPRFWRLVQTHCPDYRAHEALMSRSRGLVPDWFLLDLYR"
     gene            complement(11185..11682)
                     /locus_tag="VIW27_03785"
     CDS             complement(11185..11682)
                     /locus_tag="VIW27_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY5738819.1"
                     /translation="MARQQSTSPWVWAFLLLMLASFVAFILFLDQKIVGRGADGTVEQ
                     PPSGGETKPTIDFYTILPQRKVEIPISEEDREAIENPVINKEAVSQAILQVGSFQSAA
                     EADSLKARLAFLGLEAQIETAVVADDTWHRVQLGPFASESRLSRTKNLLIENRIEYIQ
                     RNLQN"
     gene            complement(11687..>13284)
                     /gene="argS"
                     /locus_tag="VIW27_03790"
     CDS             complement(11687..>13284)
                     /gene="argS"
                     /locus_tag="VIW27_03790"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY5738820.1"
                     /translation="PRAGQPPRELAQAIVDSLPVAEAIERVEIAGPGFINFFLASGAS
                     QSVIGRILEAGERFGRGDFGQGCRIQVEFVSANPTGPLHVGHGRGAAYGATLANLLEY
                     TGHEVQREYYVNDAGRQMHILGTSTWLRYLELCGEEIAFPSNAYQGDYIWDIAATLHR
                     EHGERFLQPADRVYDGVCADAPAGDKEKHIDELIEKARRLLGEDDYRLVFDRALDTLV
                     EVIRADLQAFGVDFDRWFSERSLADDGLIDAAIRRLDGNGHVYLQDGALWFRSSDFGD
                     EKDRVLRRDNGQTTYFASDVAYHMNKFDRGFDKVINVWGADHHGYIARVKASLAALGY
                     SADDLEIQLVQFANLFENGVKIAMSTRSGEFVTLKQLREDVGRDAARFFYVMRKADNH
                     LDFDLDLAREQSRDNPVYYVHYAHARICSVRRECAEKGIEVTSADARLERLDTSHEQA
                     LIRHLGLFPERVEAAARRREPHLVVNYLRELANFFHSWYDAHKFIVDDAELRGARLAL
                     AEAVGQVLRNGLALMGVSAPEKM"
BASE COUNT         2401 a         4268 c         4254 g         2361 t
ORIGIN      
        1 ccctgcaaat ggtcgaacac ggtatcggca ttgcgctggt cgaccacatc tccgcgcgcg
       61 aacacctcgg ccagcgcgtc gcgtttcgcc gtttcgattc accgatcagt atgaaagtct
      121 ggctgatgcg gccgcggctg cggccgcgct cgatggtggt cgatcagttc atcgagttat
      181 tgcacgcgtc ggtggatgcg ggcgagctcg acagcccgtt cgccggcaac acaccccggg
      241 gcacaggccg aggcgccacg gtcagttgag catcggctgg gatcgacgcc ggacatcgcg
      301 cgcggcgccg gctgccaacc ggatcagatg cgaaacgcgg cgaatccttt cttcggcttc
      361 gccttgaccg cgtcctgcgg gtaaagatgc gccagccagt gcttttccca caggcgaaag
      421 ccacgcgaga tcagcagcac gattaccagg tagagcaccg cggcggtgat gaaaccttcg
      481 taggcgaggt agtaacggcc gttgagccag cggccgacgc cgagcaggtc ctggatggtg
      541 atcgtgctgg cgaccaccga accgtgcagc atgaagatga cttcgttgga ataggccggc
      601 agcgcgcggc gaaacgcgct cggcatgatg atgcgccgca tgcgcatcca gcggctcagc
      661 ccgcaagcct tggcggcttc gatctcgccc ttgttggtgg tcacgatcgc gccgcgcaac
      721 agctcggtgg tataggccgc ggtattcagt gaaaacgcga tcagcgcgca ataccaggcc
      781 tgtgacagca ccgggcccca cagccaggat tcgcgaatcc actcgaactg gccgaggccg
      841 tagtaaatca gataagtctg caccagcagc ggcgtgccgc ggaacatata ggtaaacgcc
      901 cacaccggcg cattcagtat cgggttactg ccggcacgca cgatcgccag cggtatcgac
      961 agcaggccgc cgatcaccag tgagatcacg gtcagctgca gcgtgaccca ggcgcccatc
     1021 aggaagcggt cgaaattttg cgcgatcagg acgacatcga gcggcgacca ggcttcgagg
     1081 aactgaacga tcgcctcacc catcgtcggc cctcgcgata cccttgctgt actttttgtc
     1141 gagcaggcgc aggccggcgt cggagatcgc ggtcaacacc aggtagatca tcagcaccgc
     1201 gaacatgaag gtgaacagct gccgtgtcga gcggccggcg acgaagccgt tgtagaccac
     1261 gtcctgcagg ccgaccaccg agaccagcgc ggtcgccttg atctgcacca gccagttgtt
     1321 ggaaaaaccg ggcagggcgt agcgcaccat ctgcggccag atgatgcggc ggaaaatcgt
     1381 cagccgcgac atgccgcagg cgataccggc ctcgatctgg ccacgcggga tggcgaggat
     1441 cgcgccgcga aaagtctcgg tcatgtaacc gccgaatacc acgccgatgg tgccgacacc
     1501 ggcggcaaac tggctgattt cgacgtagtc ccagagaccg gtgacggcgc cgatctggtt
     1561 ggcgaactcc tgaccgccgt agaacaccag caggatcagc accaggtccg gcacgccgcg
     1621 caccacggtg gtgtaaccgg cggcggtctt ctgcgcgaga atattcttcg acagcttggc
     1681 ccaggcgccc agcagcccgc agaggacggc gatcaccagc gacagcaccg cgagccgaat
     1741 ggttacccag gtaccggcca gcagctggtc ctggtactcg atgatcagat ccacgtcgat
     1801 atttcccggc agccgaattc cggcccgtca ggcgacgggc cggatcgagt catcgccgct
     1861 cagccgccaa aaatgtcgaa atcgaaatac ttggcgttga tcttctggta gatgcccttg
     1921 tcgcggatcg ccttgatcgc ggcatcgagt tcgtcgcgca gcgcgccgtc ggacttgcgc
     1981 accgcgatac cggcaccagg gccatggcat tcctcgtcgt gctgatcgcc gccgatgaag
     2041 gcgaaaccct tgccggcgtc ggtctgcaga aagccctcgt cggccgcgat cgagtctgag
     2101 aactgcaggt cgatacgccc gatcgccagg tcctggaaga cttcgtcctg ggtaccgtaa
     2161 agcaccagct cgacaccggg atacattttc tccatgaagc actggtgcgt ggtgccgcgc
     2221 tgcaggccga cgcgcttgcc ggccaggccg cccttgctga tctcgatatc ggcacctttc
     2281 ttgccgacga acttggccgg cgtgttgtag tacttgtcgg tgaagtcgac acgcttcttg
     2341 cgttcctcgg taatcgacat cgaggcgatg atggcgtcga acttcttcgc cagcagtgcc
     2401 ggaatcatgc cgtcccagtc ctgctcgacg agtacgcagt tgcgtcccat ttccgcgcaa
     2461 atcgcctcgg cgatgtcgat atcgaatcct ttcagggtgc cgtcggattc cttccacgag
     2521 aacggcggat aagcgccttc gacgccgacg cgcaggtcag cggcctgggc gctggcgccg
     2581 ccgagcagca gcagcgcgat accgagcgtg gtgatcagtt gtttgatttt cattttttgg
     2641 ttgcctctct tcgatagtct gtagcgaaag atattatcga ttgccccacg gcccgacggc
     2701 cgcgggaaac tcactgcatc gtcctcgaca ggaattgccg aaaacgttca gaatccggat
     2761 tgcgaaatac gcgctgcggg tctccctgct cctcgatctg cccctcgtgc aggaacacga
     2821 cttccgacga aacgtcgcgc gcgaagccca tctcgtgggt cacgaccagc atggtgcggc
     2881 cctcctcggc gaggtcgcgc atgaccttca gcacctcgcc gaccagttcc gggtcgagcg
     2941 ccgaggtcgg ctcgtcgaac agcatcaccg cggggcgcat cgccagcccg cgcgcgatcg
     3001 ccgcgcgctg ctgctgaccg cccgacagct gcgccgggta ataatccttg cggtcgtgaa
     3061 tgccgacctt gtgcagcagc gcctcggctt cctcgatagc ctcggctttc ggcagcttga
     3121 gcacgtgcac cggcgcttca ataacgtttt cgagcacggt catgtgcgac cacaggttga
     3181 aactctggaa taccatgccg aggcgcgcgc gcatgcgctc gacctggcgc cggtcctccg
     3241 gctcgtaggc cccggacttg gtgtggtgca tgcgtatctt ttcaccggcc acgaaaacct
     3301 cgccggcact cggaatctcg agcaggttga tacaacgcag aaaagtgctc ttgccggagc
     3361 ctgaggcgcc gagaatcgat atcacgtcac ccttgtgtgc gcgcatcgat acgccacgaa
     3421 tgacttcgtg ctcgccgaaa ctcttgtgaa gatccacgac ctccagcgcg gcgtggccgc
     3481 cggttgcgtc gctcatgcga ttttgccctc ccgggctgac cgccatgctg gcggcaggtt
     3541 ttagttatgc gtgcatggta acactatttt tgcacccgaa tcactagtgc gggggcgtgg
     3601 cggcgctcag ccgaggctcg ccagcaagcc atcgatcata tgcccggcgc gcgccgcata
     3661 gccgccgccg aacaggttgg cgtggttgag aacatgatac aggttgtaca gatcgcgccg
     3721 ccgcgcatag ccgctgtcga tcggaaagtg atcgttatac tgctcgaaga atcgccgccc
     3781 cggactgccg aacagttcca tcatcgccaa atcggcctcg tggtcgccat aataacaggc
     3841 cggatcgaag atcaccgggt tgccggcttg gtccgccgcc tggttgccgc tccacaggtc
     3901 gccatgcaac agcgacgcgc gcggctggta atcgccgaac agcggttcga gatcggcggc
     3961 cagccggtac ccggcatcga tcacgcggct gtcgatgccg ttggcggccg ccagttgtag
     4021 ctggaactcg agccttcgtt tgcgccagaa ctcgatccag tcgtcgagaa agccattcgg
     4081 ctgcggcgta gtgccgatgg tattgtcgat ggcgaagccg aatcgcgcgt ggcaattccc
     4141 gtgcatcgcg gccagcgccg ccgccaggcg cgcggcgtcg gcacggccgc cgaagtcgat
     4201 gtattccatc acgatatagg caaatccgga caggcagccg ctggccacca ccgccggcac
     4261 gcggatcgcg ccggtggcgg caatcgctgc cagacccgcc tgctcggcct cgaacatcga
     4321 caacagcatc tcgcggttga gcttgacgaa gaactgtcga ttatcgatcc gcagccggta
     4381 ggcgcgattg atatcgccgc cgccgaccgg cgtcgcctga acctcgcgca acgccaggcc
     4441 ccgatcgcgg gcgaccatcg cggccagcac ctgccagtcc atcacaacag ccggtactcg
     4501 ccgggcgcga gaccgtcgag tgtccagtcg ccgatcgcaa tgcgcaccag gcgcagggtc
     4561 gggtgaccga cggccgcggt catgcgccgc acctggcggt tgcgcccctc tttaatctcg
     4621 agccgcagcc actgggtcgg gatatgacgg cgcacgcgaa tcggtggctc gcgcggccac
     4681 agccagccgg gctcgtcgac cgccgcgacg cgcgcaggac gcgtggtgcc gtctttcaat
     4741 tccacgccgc gagccagatc cgccagcgtg gcagcgctcg gcctgccctc gacctgcgcg
     4801 aggtaggtct tcgcggtctt gtagcgggga tcgctgatcc gcgcctgcaa cgcgccgtcg
     4861 tcggtgagca gcatcaggcc ctcgctgtcg taatcgaggc ggccggctgc atagacgccg
     4921 ggcaggtcga tgaattcggc tagcgtgcgc ttgtcgccgt cggcggaaaa ctggctgagc
     4981 acgcgaaacg gcttgttgaa caacagcgag cgggacattt cagaccctct cggccgccgc
     5041 cgattcgagc cagcgctgcc agcgcgcctg gcgcgcgtgg ccgaaatcct gctcctgcat
     5101 gatgcgctcg aggatcgccg cgtcgggctc gccacgcgcg gtctcgaccc gcgccatctc
     5161 ggccagctcg caattgatgc gcgtcagccg aatgccgacg tcgagttgcg cctggttatc
     5221 gatcagcgac tgctgcactc gcgccgcgta acgaaacttc atttcaccga cctcgtgcag
     5281 gtggcggcgc aggttatcca gcgtctggta tttcttcaac agccgcgccg cggtgcgcgg
     5341 gccaatttcc ggaatgcccg gaatgttgtc gaccttgtca ccggcgagcg ccagctgcgc
     5401 ggcgatctgc tcgggccgca cgccgaactt cttgcacacc gcgcgatagt cgagctggcg
     5461 attgtcggcg taagaccacc acaggtcgcc ctcggtgatc agctgcgcga ggtccttgtc
     5521 ggcggtgagg atcgcgaccg gcagctcctg ccggcggtga tagcgcgcca acgagccgat
     5581 caggtcgtcg gcttcccagt cgggactgct gacacagctg atgccgagcg cctccagcca
     5641 ttcgcggcag cagccgaact ggcgtttcag ttccaccggc gccggcgcgc gattggcctt
     5701 gtaatcggga tagatttctc gcctcgcgga agtcccgaga ctctcgtcga aagcgaatac
     5761 cagccgcgac ggcgcctgtt cggtgagcag gcgatagacg aaatcgctaa agccgacgaa
     5821 agcctggttg gggcgcccgt caaggtcgga gcgctcggga tcggggccga accaggccct
     5881 gaatacgtaa atactggagt cgaccaggaa caggcgcatg aactgtaatt tcaacactcg
     5941 gggttgcgca acgatactac aaccgggagc gcaaatcagg gcgcaatgga aaatcgccgc
     6001 cgagcggtta atatcctgac tatgaaagca tgccttcatg caccggctgc cgccggtcgg
     6061 ccgccgcgcc gataaacgac gcgcgcgaac tggcggccta cgtggtcgac ctgctcgagg
     6121 gcttcggccc ctgcgaggcg cggcccatgt tcggcggcca gggctttttt accagggtct
     6181 gatgttcgcg ctgatcgccg acggcaaccg ctacttaatt tggatctgac ccaaattagg
     6241 ggcgggtggc ggagagcagt acgtacttgc tgttggcggc gacggtggtg cagttgccga
     6301 acaggcggcc tagccgcagg tggtagccga ggtggcggtt gccgatcacg cgcagttcac
     6361 cgcccgcggc cagcacgcgg tgcgcatcgc ggaacatgtt gcgcgcgacg cggtcgccga
     6421 cgctgtgatg ctggtggaac ggcggattta ccagtaccag gtcggcactg ccgtcggcga
     6481 gaccctcgag gcaatcgccg aggcggaact cggcttcgcg cagcctgccg aaagcgcggt
     6541 gaaaattgtc gcgcgcactg gcgatcgcca tgtgcgactc gtccgcgaac accaggctgg
     6601 ctggcggatt gagggccgcc gcaacgatac ccagcacgcc gttaccgcaa ccgagatcga
     6661 cgatgcgccg caagccggga tcgatcggca tccgctcgat catcaggcgg ctgccgggat
     6721 ccagccgatc gcggctgaac aggctggcat ggttgctgat ttgatagctg cggtcgacga
     6781 acagttcgta actgtcggga tacgggctgc gcccttcctg taacgagtga tcgcgctcga
     6841 ccctgatcag gcgactcttc ttgcgcgcgc gggtggtggt cgccggaccg agaatggttt
     6901 cgaacagctc cagcgtcgag cgatggatgt ggcgggccat tccggcaccg agaatcagcg
     6961 tgtcgtggtg cagcacgtca cgcaggcaat aaagctggtg ctctagcagg gccagtgacc
     7021 tggggatctt gatcagcacg cagtcgaccg ccatcgccgg caaatcgata ccgctgttgg
     7081 tcgccacctg ggttgccgta tagccgttgg cgacgaggtt gtcggccagc gcgcgctgcg
     7141 cgagacaaga gtcgttccac gagtagagcg ggtgatcggc gagcgccacc gccagggcgc
     7201 caaaggcgtc gttgagcaac agcagcttgc cgtgccgcga tggcacccgg cattcgtcca
     7261 ggtagccgag caggtactcg tcggccgcgt cccaggcctg caggcgctgg tcgcgcgggt
     7321 tgcgttgcag gacgaactcg ccctgcggca ccagcagcgt tcccggccgc tcgctcgggt
     7381 aaggctggtt catggccggg taaacggcaa ggccagcatg cctggcaggc ggtcgacccg
     7441 ccgcgggtcg cggaactcgt tgagaccgat gctcatgccg tcgtgtccca gttgcggctg
     7501 atcgatgtag gtgccgaaaa ttcgatccca gaccgacagg ttgaaaccgt agttgctgtt
     7561 ggtttcggtg acgcgagtcg aatggtgcac gcggtgcatg tcaggggtca ccaacaggcg
     7621 gcgcaataca cggtcggcga gcgcgggcag gcgcacgttg gcgtggttga acaatgccat
     7681 gccattgagc agcacctcga atatcagcac cgccagcagc gccgggccga gtagcaggat
     7741 caccccccac ttgaacagca tcgacaacag gatctcgatg ctgtggaatc tcgacccggt
     7801 ggtcaggtcg atgtcgaggt cggcatggtg cacgcggtgc aggcgccaca acagcggcac
     7861 ccggtgcatc agcaaatgct gcaggtaaat cgacaggtcc agcaacagca ccgcagcgac
     7921 gacctcgagc cagtagggca gcgcgacgcg gttgaacaac ccccattgca ggtcggcggc
     7981 cgaataggcc agtccgaccg cggcggccgg gaacagcagg cgcagtacca cggagttgat
     8041 cgccagcagg ccgagattgt taacccagcg cagcggcttg gaaaccgtca gccggcggcg
     8101 cggcgacagc cactcccata gccccatgac caggaataca gcgatgaaaa cagccagccg
     8161 tatgccggcc tcgttatcga tcgcaaaggt ttccatcgcc tgttccatga cgcgcattgt
     8221 cgagcaaaag tcgcaaattt gaaacccggc ggcggcggga cgcgacaggc acatcgatcg
     8281 agactggctc ggcgatgctg cgggcgcctg acgataatgc tcgcagcacc ggcgcaacga
     8341 attagcggcc gaccgggtgg cgattcgagc gcggattgat agcggcattt cacgcgcggt
     8401 ttcgatccgc catttcattt cccgagcatg ggtgtgttat acaggcgcgg atcatcgaaa
     8461 atccggggaa ccatgctcaa cgacattctg ctgctgctga ttctttccgc tgtcagcgtc
     8521 gcgattttcc gccgcatcga cgtcccggca gtgctcggct acctggtcgt cgggctgctt
     8581 gccggcgacc gcgcgctggg gctgatacac gattcgcatg cgatcgagca gatcgccgaa
     8641 atcggcgtcg ttttcctgct gttcgcgatc ggcctggaag tgtcgattcc gcgactgctg
     8701 gcgatgcgca atatcgtctt cggtatcggc ctgacccagg tcgtaatttc gaccctgagc
     8761 acgctggtga tcggcatggc tttcggcctg tcgtggcagg cggccttcgc ggtcggcggc
     8821 gcgctggcga tgtcgtcgac ggcggtggtg accaagctgc tcaccgagca gtacgagctg
     8881 caccagccgc acggcaacat ttcgctgggc gtgctgctgt tccaggatct cgcggtggtg
     8941 cccttcctgg tgctgattcc gatcctcgcg gcgcccggcg agggatcgct gctggtgccg
     9001 atcagcctgg cgctggccaa gggcgcgctc gccttcgcgg ttatctttta cgccggccag
     9061 tacctgttgc gacccgcggt gcgcaaggtc gcggcgaccc actccagcga gctgttcacg
     9121 ctgttcgtgc tgtcgaccgc gttgatcgcg gcctggctga cctggcaact cgggctgtca
     9181 ctggcgatgg gtgcctttct cgccggtatc atgctcgcgg aaaccgagta ccagcatcgc
     9241 atcgaaaacg agatcaggcc gtttcgcgac gtgttgatgg gcctgttctt catttccgtc
     9301 ggctcgcagt tcgactggca ggtgatcatc aacgagccct tcgaggtcgc cgttctgact
     9361 gtcggcctgg tgttcggcaa ggggttcgcg atcacggtga tttccgcgct ggccggttac
     9421 gcgcgcgata tcgcgatccg tgccgggatt ctgctcggcc agggcagcga attcggcttc
     9481 gcggtgctcg cggttgcgat aaccggcggc ctgctcggcc ttgagccgag ccaaccgatc
     9541 attgcggcga ttattctcag catggtgata tcgccgatga tgattcgcca taacgcggcg
     9601 atcgcgcagc gcatcgcgcc cgccgccgaa aaccttccca acccgtcgga aaccgcactc
     9661 gaagaagcct gccgggatct acaggaccac gtgctgctgt gcggcttcgg ccgcaccggg
     9721 caaaacctcg gacagttcct gcgccaccag ggcattccgt tcattgcact cgaaatcgag
     9781 gcggatatcg tcaacgaggc gcgcgaggcc ggcgagcccg tgtttatcgc cgacagcggc
     9841 aacccgcgga ttctgaagct cgccggcatc gacaaggccc gggtgctggt gatttcgttc
     9901 accgaggtcg agccggccga aaatatttgc cggcaagtca gggaactgcg ccccgacatt
     9961 ccgctgatca tccgcacccg cgacgaccgc cacctcgaac acctgctgga gtgcggcgcc
    10021 gacgaagtca ttccggacac ggtcgaatcc agcatcatgc tggccaggca tacgctgacc
    10081 gcgctcggcg aaagtggccc cgatatggac catttgctcg aacaggcgcg tgccggccac
    10141 tacgcccgaa tacgcgcgtt ctttcacagc gtgcgcgacg tcaatctcgg ggctgccgac
    10201 cgccaccacc tgcacagcgt cgagattctg ggcacctatt acgcggtcgg gcgcagtatc
    10261 gataccatgg agcggctgca gcaggtgcgc atcatcacca tacgccgcaa cggcgtcagc
    10321 agcgaagacc cgccgccgga gctggaactg aagaccggcg acgtactggt catcgaaggc
    10381 cgtcccgccg atattcgggc ggcggagatt caactcatgt cgggattata ggagccgcag
    10441 ctgacacacc cggacgacag gccagagccc ctgcgctggg aattgcgggt ggcgccgcgc
    10501 gcgcgacgcg caaaattgcg aattcggccc ttcggcggtc tcgaggtcgt gattccgccg
    10561 cggtttccgc gcgcgcagat cgcggcgctg gtggagcggc acgccacctg ggcacggcgc
    10621 cagctcgatc ggcaagcccg cctgcgcgac gcgattcgcc tgccgcagca tctcgatctc
    10681 gcctacgaca acagcagcac accggtaatc tacgcgaccg aaccgctgac ctggaccggc
    10741 gacctgttcg ccgagccgtc acaggcgccg atcgtcgtcg aggccgcgga ccaggcggcg
    10801 cggattggcg agctgcgcgg ctggatcagg cgccgcgcgc accagctgct gccgccgaag
    10861 ctgcaggcac tgtccaggcg caccgggctc gaatacgccg gcgtcggcat tcgcagccag
    10921 aagacccgct ggggcagttg ctccagccgc ggccatatca gcctcaacga tcaattgctg
    10981 ttcctgcccg cggacaccgt cgactacctg atgatccacg agctgtgcca cacccggcac
    11041 ctcgatcact caccgcgttt ctggcgactg gtgcaaaccc attgcccgga ctaccgcgcg
    11101 cacgaggcgc tgatgagccg ctcgcgcggc ctggtgcccg attggttcct gctcgacctg
    11161 taccgctgag tcgcgggcgc cgaatcaatt ctgcaggttg cgctggatat actcgattcg
    11221 gttttcgatt agcaggttct tggtgcgcga caggcgcgat tcgctggcga acggccccag
    11281 ctgcacgcgg tgccaggtat cgtcggcgac caccgccgtc tcgatttgcg cctcgagccc
    11341 gagaaatgca agccgcgcct tcaggctgtc ggcctcggcg gcgctctgaa aggacccgac
    11401 ctgcaatatg gcctggctca cggcttcctt gttgattacc gggttttcga tcgcctcgcg
    11461 gtcttcctcg gagatcggga tctcgacctt gcgctgcggc aagatggtgt agaagtcgat
    11521 cgtcggcttg gtttcgccac cgctcggcgg ctgctccacg gtaccgtcgg cgccgcggcc
    11581 gacgatcttt tgatcgagga agaggataaa ggcgacgaac gacgccagca tcagcagcag
    11641 gaaggcccac acccacggcg aggtcgactg ttggcgggcc atggcgctac atcttctccg
    11701 gtgccgagac gcccatcagg gcgaggccat tgcgcagcac ctggccgacc gcctcggcca
    11761 gcgcgaggcg cgcgccgcgc agctcagcgt cgtcgacgat gaacttgtgc gcgtcgtacc
    11821 aggagtgaaa aaagttggcg agctcgcgca ggtaattgac caccaggtgc ggctcgcggc
    11881 gcctggccgc cgcttcgacg cgttcgggaa acaatcccag gtgcctgatc agtgcctgct
    11941 cgtgactggt gtcgagacgc tcgagcctgg cgtcggccga cgttacctcg atgccttttt
    12001 cggcgcattc gcggcgcacg ctgcaaatgc gcgcgtgcgc gtaatgtacg tagtaaaccg
    12061 ggttgtcgcg gctctgctcg cgcgccaggt cgaggtcgaa atcgaggtgg ttgtcggcct
    12121 tgcgcatcac gtagaaaaaa cgcgccgcgt cgcggccgac gtcctcgcgc aactgcttga
    12181 gcgtgacgaa ttcgccggat cgcgtcgaca tcgcgatctt gacgccattc tcgaacaggt
    12241 tggcgaactg caccagctgg atctcgagat cgtcggcact gtaaccgagc gccgccagcg
    12301 acgccttgac gcgtgcgatg taaccgtggt gatccgcgcc ccagacgttg atcaccttgt
    12361 cgaagccgcg atcgaacttg ttcatgtggt aggcgacatc ggaagcgaaa taagtggtct
    12421 ggccgttgtc acgccgcagt acgcggtctt tttcgtcgcc aaagtcgctg gagcggaacc
    12481 atagggcgcc atcctgcaga tagacgtggc cgttgccgtc gagccgccgt atcgcggcat
    12541 cgatcaagcc gtcgtcggcc agcgagcgct cggagaacca gcggtcgaag tcgacgccaa
    12601 aggcttgcag gtcggcgcga atcacctcca ccagcgtgtc gagggcgcgg tcgaaaacca
    12661 ggcggtaatc gtcctcgccg agcaggcggc gagccttttc gatcagctcg tcgatatgct
    12721 tttccttgtc gccggcgggc gcgtcggcac acaccccgtc gtatacccgg tcggcgggct
    12781 gcagaaaacg ctcgccatgt tcacggtgca gcgtggcggc gatatcccag atgtagtctc
    12841 cctggtaggc gttgctcgga aaggcgattt cctcgccgca cagctcgagg tagcgcagcc
    12901 aggtcgaggt accgagaata tgcatctgcc gaccggcatc gttgacgtaa tactcgcgct
    12961 gcacctcgtg gccggtgtac tccagcaggt tggcgagcgt ggcgccgtag gcggcgccgc
    13021 ggccgtgacc gacgtgcaac ggcccggtgg gattggcgga aacgaattcg acctggatac
    13081 gacaaccctg accgaaatca ccgcggccga agcgctcccc ggcctcgagg atgcggccga
    13141 tcaccgactg actggcgccg ctggcgagga aaaaattgat gaatccgggg ccggcgatct
    13201 ccacccgttc gatggcctcg gccaccggca ggctgtcgac gatggcctgc gccagctcgc
    13261 gcggcggctg cccggcgcgc gggg
//
LOCUS       DATCZV010000170         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_171811, whole genome shotgun sequence.
ACCESSION   DATCZV010000170 DATCZV010000000
VERSION     DATCZV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171811"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VIW27_03795"
     CDS             <1..1001
                     /locus_tag="VIW27_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY5738821.1"
                     /translation="YMTELHRYKQLNRQLLAMRGQPIRLHLDGEERLTVEKTDVMLEA
                     LATSLQIHLQVPFDEIVPTYHAGLWASMLILAATANSPLVLGKCCWQESRIGIFKQAV
                     DTRNPQEIENHIIPRVHLGKGYIASLLDLFEDNFYYSPILPEVRERKPEDLHHLCLHN
                     GTIWRWVRPIVAHDGDGQYHLRLELRVVPSGPTLVDTIANLVLYVGLTEGLKTLGEEL
                     TRVPFATLEADFYSAARHGLEAAAHWPDGREAPLRQLLLEDALPLARDSLGRAGIEDG
                     GRWLDIIEARVDSGATGARWISGHWKRHRDAPRLVREYITRAQTNRPVHLWQDPGE"
     gene            998..2017
                     /locus_tag="VIW27_03800"
     CDS             998..2017
                     /locus_tag="VIW27_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY5738822.1"
                     /translation="MIETFERLHEMPDGFFDIDSANICRLFPHPTLIHLEGRDSRHLF
                     VSILLHGNEVTGLQVMQSLLAGYADGLPRSLLLFIGNVRAAEQNRRFLPGQVDYNRCW
                     PGGDLVDDPSARMMQRVIEIAHGLPLFAAVDIHNNTGRNPHYACITDPRPENQNLAAM
                     FNRVGIVFRQRGVSTMAFNGICPAVTLECGLPGEPSGTEHARRYLEELLLLDELPTRQ
                     PTRDELQLVESHLMLRIPEDVSFEFDPDADVDLRFEADFENRNFTLFDPRQVFGYTRL
                     ERPLTAIDTDGHDLTHEILRVEQGRIYLNNTLMPAMITRDKLVVRQDCLCHLLQDYLP
                     HEEVF"
     gene            2092..>2426
                     /locus_tag="VIW27_03805"
     CDS             2092..>2426
                     /locus_tag="VIW27_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738823.1"
                     /translation="MIKPVYAAVLALLLAALAPLAAAADGAPDSAGRTLSPAALLGAA
                     GDGDFARAELPYPFRFPADHGSHDAYRTETWQLFGVLESGDRGPLGMQLSVIRMALGD
                     SSAAAGWATG"
BASE COUNT          432 a          818 c          762 g          414 t
ORIGIN      
        1 gatacatgac cgaattgcat cgttacaaac agctcaaccg gcaattgctg gcgatgcgcg
       61 gccagccgat tcgcctgcat ctcgatggcg aggaacggct gaccgtggaa aaaaccgacg
      121 tcatgctcga agcgctggcg acctcgctgc agattcacct gcaggtgccg ttcgacgaaa
      181 tcgtgcccac ttaccacgcc ggcctgtggg cgagtatgct gatcctcgcc gcgaccgcca
      241 actcgccgct ggtgctcggc aagtgttgct ggcaggaatc gcgcatcggt atcttcaagc
      301 aggcggtcga tacccgcaat ccgcaagaaa tcgaaaacca catcattcca cgcgtgcacc
      361 tcggcaaggg ctacatcgcg tcgctgctcg atctgttcga agacaacttt tactacagcc
      421 cgatcctgcc cgaggtgcgc gagcgcaagc ccgaggacct gcaccacctg tgcctgcaca
      481 acggcaccat ctggcgctgg gtaaggccga tcgtcgccca cgacggcgac ggccaatatc
      541 atctgcggct cgagctgcgc gtagtgccgt cggggccgac gctggtcgac acgatcgcca
      601 acctggtgct ctacgtcggc ctgaccgagg gcctgaagac gctcggcgag gagttgaccc
      661 gcgtgccatt cgcgactctc gaggccgatt tctacagtgc cgcgcgacac gggctagagg
      721 ccgcggccca ctggcccgac ggccgtgaag cgccgctcag gcagctgctg ctcgaagacg
      781 ccttgccgtt ggcgcgtgac tctctcggcc gcgccggtat cgaagacggc ggccgctggc
      841 tggatatcat cgaagcccgc gtcgacagcg gcgcgaccgg tgcgcgctgg atcagcgggc
      901 actggaagcg ccaccgcgac gcgccgcggc tggtgcgcga gtatatcacc cgggcgcaga
      961 ccaaccgccc ggtgcacctg tggcaggatc cgggagaatg atcgagacct tcgagcgtct
     1021 ccatgaaatg cccgacggct tcttcgacat cgacagcgcg aatatttgcc ggctgtttcc
     1081 gcaccccacg cttatccatc tcgagggccg cgattcgcgc cacctgttcg tgtcgatcct
     1141 gctgcacggc aacgaggtca ccggcctgca ggtgatgcag tcgctgctgg caggctatgc
     1201 cgacggtctg ccgcgctcgc tgctgctgtt catcggtaac gtgcgcgcgg ccgagcaaaa
     1261 ccggcgcttt ctgcccggac aggtggacta caatcgttgc tggccgggcg gcgacctggt
     1321 cgacgatccg agcgcgcgga tgatgcagcg ggtgatcgag atcgcgcatg gattgccgct
     1381 gttcgcggcg gtcgacattc acaacaatac cggccgcaac ccgcactacg cctgcattac
     1441 cgacccgcgg ccggaaaacc agaacctggc ggcgatgttc aaccgcgtcg gcatcgtgtt
     1501 ccgccagcgc ggcgtcagta cgatggcttt caacggtatc tgcccggcgg tcaccctcga
     1561 atgcggcctg cccggagagc cgagcggtac cgagcatgcg cgccgctacc tcgaagagct
     1621 gttgctgctc gacgaactgc cgacccgcca accgacgcgc gacgagttgc aactggtcga
     1681 atcgcacctg atgctgcgga tccccgagga cgtcagtttc gagttcgatc cggatgccga
     1741 cgtcgatctg cgcttcgagg ccgatttcga gaatcgtaat ttcacgctgt tcgatccgcg
     1801 gcaggtgttc ggctacacgc gcctcgagcg accgctgaca gcgatcgata ccgacggcca
     1861 cgacctgacg catgaaatcc tgcgtgtcga gcaagggcga atctatctca acaatacgct
     1921 gatgccggcg atgatcactc gtgacaagct cgtcgttcgc caggattgcc tgtgccattt
     1981 gctgcaggat tacctgcccc acgaggaggt gttctgaagc accgtcgggg catgtcacgg
     2041 gaagcgcaga acgcctctgc tggccgccgg caacgctgga cataaaccgc gatgatcaaa
     2101 cccgtttacg ccgccgtcct cgcgctgctg ctcgccgccc tcgccccgct cgcggcggcg
     2161 gccgacggcg ccccggatag cgcggggcga acgctgtcgc cggccgccct gctcggcgcc
     2221 gccggcgacg gcgatttcgc gcgcgccgag ctgccctacc cgtttcgatt tccggccgac
     2281 cacggcagcc acgacgccta ccgcaccgaa acctggcaac tgttcggcgt gctcgagagc
     2341 ggcgatcggg gtccactggg catgcaactg tcggtgatcc gcatggcgct gggcgacagc
     2401 agtgcagcgg ccggctgggc gacggg
//
LOCUS       DATCZV010000171         6059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_701141, whole genome shotgun sequence.
ACCESSION   DATCZV010000171 DATCZV010000000
VERSION     DATCZV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701141"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2475
                     /gene="carB"
                     /locus_tag="VIW27_03810"
     CDS             <1..2475
                     /gene="carB"
                     /locus_tag="VIW27_03810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5738824.1"
                     /translation="PAQTLTDKESQIMRNASIAVLREIGVETGGSNVQFAINPENGEM
                     IIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNDITGGATPASFEPTID
                     YVVTKIPRFTFEKFPQADDRLSTQMKSVGEVMAIGRNFQESFHKALRGLETGVFGLDP
                     IVDQSQDAEELEDLYRTELRLPGAKRIWYVADAFRSGYSLEQVFQQTAIDPWFLVQIE
                     DLVRIEGSLGGRQLEDLDAVEMRALKRKGFADRRLAELLGCEETAFAARRRELGVRPV
                     YKRVDTCAAEFATSTAYMYSSYDEECEAMPSARDKIVVLGGGPNRIGQGIEFDYCCVH
                     AALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTYEDVLEIIHLEQPRGVIVQF
                     GGQTPLNLARALEAAGVPIVGTTPDSIDLAEDRERFQKLIQELGLRQPPNQIAFDVEA
                     ASRLAEQVGFPLVVRPSYVLGGRAMEIVYDQEDLARYMSEAVKVTRDSPVLLDHFLDE
                     AVEVDVDAICDGERVLIGGLMEHIEQAGVHSGDSACSLPPYTLSAEIQQQLCEQVGAM
                     ARALNVVGLMNTQFAIKGDEVYVLEVNPRASRTVPFVSKASGVQLAKVAARCMVGQTL
                     EQQGRTEQVVPEFVSVKESVFPFTKFQGVDPILGPEMKSTGEVMGIGSNFAEAFAKAM
                     RGSGAEIPTPGTAFLSVREADKEGIVEIARQLHAQGFSLVATGGTGKVIRDADIECLH
                     INKVKEGRPHIVDMIKNDEIALIVNTTEGRSAIADSYTIRREALMHAVCYTTTLSGAR
                     ATCQAMAHREIKQVYCIQTLHQAVEQ"
     gene            2472..2948
                     /gene="greA"
                     /locus_tag="VIW27_03815"
     CDS             2472..2948
                     /gene="greA"
                     /locus_tag="VIW27_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY5738825.1"
                     /translation="MKQTPITAAGAERIRDELRELKSVKRPAVIDAIATARDHGDLKE
                     NAEYHAAREEQGFIEGRIAELEAALAHAQIIDITRIPASTRAVFGCTVTLHDVASENE
                     ITYQIVGDIEADINENRIAISSPIARALIGKETGEDVIVEAPAGTMEYIITAVEYK"
     gene            complement(3371..3628)
                     /locus_tag="VIW27_03820"
     CDS             complement(3371..3628)
                     /locus_tag="VIW27_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY5738826.1"
                     /translation="MKLKPVVIIGQKGLSENVQNEIDGALTHHELVKLRLPALDRVER
                     RELCEQLCHMHSATLVEAIGGIAVFYRRNPDTDRFASLLKS"
     gene            3603..4364
                     /gene="rlmE"
                     /locus_tag="VIW27_03825"
     CDS             3603..4364
                     /gene="rlmE"
                     /locus_tag="VIW27_03825"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HEY5738827.1"
                     /translation="MMTTGFSFIRCPSARSCLICPLDSFILRVRERRPACILTQPRRA
                     TAVPRSKSSKQWLERHQRDEYVQKARAEGLRSRAVYKLEQIQQRDHILAPGQTVLDLG
                     AAPGGWSEYASRVVGPRGRVLAVDMLPMDAIADVEFLQGDFTEQQTLDELLALLGDHP
                     LDLVLSDMAPNLSGVQSVDQPKSIYLVELALDLARRQLQPGGGFVAKLFQGEGFDRLV
                     AELRADFVSVKLRKPAASSSRSSEIYAVCSGLRQA"
     gene            4454..>6059
                     /gene="ftsH"
                     /locus_tag="VIW27_03830"
     CDS             4454..>6059
                     /gene="ftsH"
                     /locus_tag="VIW27_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5738828.1"
                     /translation="MNDVVKNLVLWIVIAVVLLSVFHSFGERGSNAQTLIYSEFLTQV
                     EQGQVREVTIEGQNIAGKMISGQPFTTFSPETNNSALIGDLKRADVAVVGKPPEPPSL
                     LGHMFISWFPFIVLIGLWIFFMRQMQGGSGGRGAMSFGKSKARLLGEDQINISFNDVA
                     GVDEAKEEVAELVEFLRDPSKFQKLGGKIPRGVLMVGSPGTGKTLLAKAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFDQAKKHSPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGRSQ
                     ILKVHMRKVPAAENVDPMVIARGTPGFSGADLANLVNEAALFAARANKKLVTMGEFER
                     AKDKIMMGAERKSMIMKEDEKKLTAYHEAGHAIVGRLVPEHDPVYKVSIIPRGRALGV
                     TMFLPEDDRYSFSKKHLESQLSSLFGGRIAEQQIFGEDAVTTGASNDIERATSIARSM
                     VTQWGLSDRMGPLSYSEEEGEVFLGKS"
BASE COUNT         1219 a         1779 c         1936 g         1125 t
ORIGIN      
        1 ccggcgcaga cgctgaccga caaggaatcc cagatcatgc gcaacgcctc gatcgcggtg
       61 ctgcgcgaga tcggggtgga gaccggcggt tccaacgtgc agttcgcgat caatcccgaa
      121 aacggcgaga tgatcatcat cgagatgaat ccgcgcgtgt cacggtcgtc ggcgctggcc
      181 tccaaggcca ccggcttccc gatcgccaag gtcgccgcca agctggcggt cggctacacg
      241 ctcgacgaac tgaaaaacga tatcaccggc ggtgccacgc cggcgtcctt cgagccgacc
      301 atcgattacg tggtcaccaa gattccgcgc ttcaccttcg agaaattccc gcaggccgac
      361 gaccggctgt cgacgcagat gaaatcggtc ggcgaggtga tggcgatcgg gcgcaatttc
      421 caggaatcct ttcacaaggc gctgcgcggc ctcgaaaccg gcgtcttcgg cctcgacccg
      481 atcgtcgacc agtcgcagga tgccgaggaa ctcgaggacc tgtatcgcac cgagctgaga
      541 ctgccgggcg cgaagcgcat ctggtacgtg gccgatgcgt ttcgctcggg ttacagtctc
      601 gagcaggtct ttcagcagac cgcgatcgat ccgtggttcc tggtgcagat cgaggatctg
      661 gtgcgcatcg agggcagcct cggcggccgt cagctcgaag atctcgacgc cgtcgagatg
      721 cgcgcgctga agcgcaaggg atttgccgac cgacggctgg cggaactgct cggctgcgag
      781 gagaccgcgt tcgccgcgcg gcgccgcgaa ctcggcgtgc gcccggttta caagcgcgtc
      841 gacacctgtg ccgcggaatt tgccaccagc accgcctaca tgtattcgag ctacgacgag
      901 gaatgcgagg cgatgccttc cgcgcgcgac aagatcgtcg tcctcggtgg cggcccgaac
      961 cggatcggcc agggtatcga gttcgattac tgctgtgtgc acgcggcgct ggcgatgcgc
     1021 gaggatggtt acgagaccat catggtcaat tgcaacccgg agaccgtgtc caccgactac
     1081 gacacctcgg accggctgta cttcgagccg ctgacctacg aggacgtgct ggagatcatc
     1141 cacctcgaac agccgcgcgg cgtgatcgtg cagttcggcg gtcagacacc gctcaacctg
     1201 gcgcgcgcgc tggaggcggc cggcgtgccc atcgtcggta ccacgcccga ctcgatcgat
     1261 ctcgccgagg accgcgagcg ctttcagaaa ttgatccagg agctgggtct tcggcagccg
     1321 cccaaccaga tcgcctttga cgtcgaagcg gcgtcgcggc tggccgagca ggtcggcttt
     1381 ccgctggtgg tgcggccgtc ctacgtgctt ggcgggcggg cgatggaaat cgtctacgac
     1441 caggaggacc tggcgcgcta catgagcgag gcggtcaagg tgacgcgcga ttcgccggtg
     1501 ttgctggatc actttctcga cgaagccgtg gaggtcgatg tcgacgcgat ctgcgatggc
     1561 gagcgggtgc tgatcggcgg tttgatggag cacatcgagc aggccggcgt gcattccggc
     1621 gattccgcct gctcgctgcc gccgtatacg ctgtcggcgg aaatccagca gcaactgtgt
     1681 gagcaggtcg gcgccatggc gcgcgcactg aacgtcgttg ggctgatgaa tacgcagttc
     1741 gcgatcaagg gcgacgaggt ctacgtgctc gaggtcaacc cgcgcgcctc gcgtaccgtg
     1801 ccgttcgtct ccaaggccag cggcgtgcag ctggccaagg tcgccgcgcg ctgcatggtg
     1861 ggacagactc tcgagcagca gggccgtacc gagcaggtgg tgccggagtt cgtctcggtc
     1921 aaggaatcgg tttttccgtt caccaagttc cagggtgtcg acccgattct cggcccggag
     1981 atgaaatcca ccggcgaagt gatgggcatc ggcagcaatt tcgccgaggc ctttgccaag
     2041 gccatgcgcg gttcaggagc agagataccc acgcccggaa cggcattcct gagcgtgcgc
     2101 gaagccgaca aggagggtat cgtcgagatt gcccgccaac tgcatgcgca gggcttttcg
     2161 ctggtggcca ccggcggcac cggtaaggtg attcgcgatg ccgacatcga gtgcctgcat
     2221 atcaacaagg tcaaggaagg gcgtccgcac atcgtcgaca tgatcaagaa cgacgaaatc
     2281 gcgctgatcg tcaacaccac cgagggacgt tcggcgattg ccgattccta caccattcgt
     2341 cgcgaggcgt tgatgcatgc ggtatgctat acgacgacct tgtccggggc gagggccacc
     2401 tgtcaggcaa tggcccaccg cgagataaaa caggtatatt gcatccaaac cctacatcag
     2461 gcagtcgaac aatgaagcag acaccgatta ccgcggccgg cgcggagcgg atcagagacg
     2521 agctcagaga gctgaaatcg gtcaagcgac cggcggtgat cgacgcgatc gccaccgcgc
     2581 gcgaccacgg cgacctgaag gaaaacgccg agtaccacgc ggcgcgcgag gagcagggct
     2641 tcatcgaggg gcgtatcgcc gagctcgaag cggcgctggc gcacgcgcaa atcatcgata
     2701 tcacgcgaat tccggcatct acgcgcgcgg tattcggctg caccgtgacg ctgcatgacg
     2761 tcgccagcga aaacgaaatc acttaccaga tcgtcggcga catcgaggct gacatcaatg
     2821 agaaccgcat cgcgatatca tcaccgattg cgcgcgcgct gatcggcaag gaaaccggcg
     2881 aggacgtcat cgtcgaagcc cccgccggaa ccatggaata catcatcacc gccgtcgaat
     2941 acaagtaatt cctgtaccgt aaaccactac acgattgtcc cttcgatggc gcggccgctc
     3001 gagaaacttt gcgagggacg ccgaaaatac ctccatctag gctcgtgcgc gccatccagg
     3061 gcgcgcaacg cccttgcaaa gtttctcggg cgtccacgcc ctacattgat actgatctca
     3121 acaatttaga gcttcggcag gaaggcgctc catttgggca agctgtaacg ggttgagaga
     3181 atcgaatact ggacgctctt gcagaggcaa tcgaagcagc cactcgcttc cattcaggtc
     3241 agttttcgtg gagacacagg ccaccgggcc gggcgcgagt ggctgcttcg gttgccgtat
     3301 gggaaatatt ttcgaacgtg acaactgagg atgccgaggc tatcgggcgc gctttttgct
     3361 ttatgaaatg ttaagacttg aggagggaag cgaagcggtc ggtgtcggga ttgcggcggt
     3421 aaaagacggc gatgccgccg atggcttcga cgagggtggc gctgtgcata tggcagagtt
     3481 gttcgcagag ttcgcggcgc tcgacgcggt ctaacgccgg cagccgcaac ttgaccagtt
     3541 cgtggtgtgt cagcgcgccg tcgatttcgt tttgcacgtt ttcagacagg cctttctggc
     3601 cgatgatgac caccggtttc agtttcatcc gatgcccttc ggcgcgcagc tgcttgattt
     3661 gtccactgga tagtttcatt ctgagggttc gagaaagacg accagcatgc attttaacac
     3721 agccccgacg cgcaactgcc gtgcccagat cgaaatccag taaacaatgg ctcgagcgcc
     3781 atcaacgaga tgaatatgtg cagaaagcgc gtgccgaggg attgcgttcg cgcgcggttt
     3841 acaagctcga gcaaatccag caaagagacc atattcttgc gcccggccag acggtgctcg
     3901 atcttggtgc ggcgccgggc ggctggagcg aatacgcgag ccgcgtggtc ggtccgcgcg
     3961 ggcgcgtgct ggcggtggac atgctgccga tggacgcgat tgccgatgtc gagttcctgc
     4021 agggagactt taccgagcag caaaccctcg acgaattgtt ggcgctgctc ggcgatcacc
     4081 cgctcgacct tgtattatcc gacatggccc ccaatttgag tggggtacaa agcgtggatc
     4141 agccgaaatc catttacctg gtggaactgg cgctcgacct cgcccggcgg caactgcaac
     4201 cgggcggggg atttgtcgcc aagcttttcc agggcgaggg gttcgatcgc ctggtcgctg
     4261 agctgcgtgc ggacttcgtc tcggtcaaat tgcgcaaacc ggccgcatcc agttcacgat
     4321 cgagtgaaat ttatgcggtt tgcagcggtc tgagacaggc ttagtcacgt aaaatacgct
     4381 agtatctggg tttattagca gtaaaatgaa tcgcggagct ggcaataagt agttagccaa
     4441 agcaggtgga atagtgaacg acgttgtaaa gaatttggta ttgtggatag tcatcgcggt
     4501 ggtattactg tcggtattcc acagtttcgg tgagcgcggc agcaatgcgc aaaccctgat
     4561 ttactcggaa ttcctgaccc aggtcgagca ggggcaggtg cgcgaggtca ccatcgaggg
     4621 tcagaatatc gccggcaaga tgatttccgg ccagccgttc acgaccttca gcccggaaac
     4681 caacaatagt gcgctgatcg gcgatctcaa gcgtgccgat gtcgccgtgg tcggcaagcc
     4741 gcccgagccg ccgtcgctgc tcggccacat gttcatcagc tggttcccgt tcatcgtact
     4801 gatcggcctg tggatattct tcatgcgcca gatgcagggc ggcagcggtg gtcgcggcgc
     4861 gatgtcattc ggcaagagca aggctcgcct gctcggcgaa gaccagatca atatctcttt
     4921 caacgatgtc gccggcgtcg acgaggccaa ggaagaagtg gccgagctgg tcgaattcct
     4981 gcgtgatccg tcgaaattcc agaagctggg cggcaagatt ccgcgcggcg tgctgatggt
     5041 gggttcaccg ggtaccggta aaaccctgct cgccaaggcc atcgccggcg aggccaaggt
     5101 gcctttcttc accatttccg gctccgactt cgtcgaaatg ttcgtcggtg tcggcgcctc
     5161 gcgggtgcgc gacatgttcg atcaggcgaa gaagcactcg ccgtgcatca tcttcatcga
     5221 cgagatcgac gcggtcggcc gtcatcgcgg cgccggcctc ggcggcggtc acgacgagcg
     5281 cgaacagacc ctgaatcagc tgctggtcga aatggacggc ttcgagggca atgagggtgt
     5341 gatcgtcatc gccgcgacca accgtccaga cgtgctcgat ccggcattgc tgcgcccggg
     5401 ccgcttcgac cgccaggtgg tggtgccgct gcccgacgtg cgtggccgct cgcagatcct
     5461 caaggtgcac atgcgcaagg tgccggcggc ggaaaacgtc gatccgatgg tgatcgcgcg
     5521 tggcacgccg ggattttccg gtgccgatct cgccaacctc gtcaacgagg cggcgctgtt
     5581 cgcggcgcgc gccaacaaga agctggtgac gatgggcgag ttcgaacgcg ccaaggacaa
     5641 gatcatgatg ggcgccgagc gcaagtcgat gatcatgaag gaagacgaaa agaagctgac
     5701 tgcttatcac gaagccggtc acgcgatcgt cggccgcctg gtgccggagc acgacccggt
     5761 ttacaaggtc agcatcattc cgcgcgggcg cgcgctcggg gtgaccatgt tcctgccgga
     5821 agacgatcgt tatagtttca gcaagaagca cctcgagagc cagctgtcca gcctgttcgg
     5881 cggccgaatc gccgagcagc agatattcgg cgaggacgcg gtgaccaccg gcgcctcgaa
     5941 cgatatcgag cgcgccacgt cgatcgcgcg cagcatggtc acccagtggg gcctttcaga
     6001 tcgcatggga cccctgtcct atagcgagga agagggcgag gtatttctcg gcaagtcct
//
LOCUS       DATCZV010000172         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_225113, whole genome shotgun sequence.
ACCESSION   DATCZV010000172 DATCZV010000000
VERSION     DATCZV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225113"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VIW27_03835"
     CDS             <1..753
                     /locus_tag="VIW27_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY5738829.1"
                     /translation="SWNGEHIGAGRLTHIGLRANSAIAQQIEFTRPFKAHHRVAWSFE
                     SRGDKTLASWEMNGRMPLLKRFMAARIEPMIGRDYELGLALLNGFLNADAEHLRLTFD
                     GREQLEDFSYWSIPCHGNLRQLEANRDASLDALQRAAAGKTGLGLTLYRGFDPLVAHY
                     RAEFAIPVVSTTPDSNYTRREFHGGTYLRMTLRGGHQFLPLGWHALYSHCRLLGLKTD
                     RARPSLEIYHTTPAQTVDSNQVTTVLYAPLKA"
     gene            complement(765..1819)
                     /locus_tag="VIW27_03840"
                     /pseudo
     CDS             complement(765..1819)
                     /locus_tag="VIW27_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            complement(1902..>2835)
                     /locus_tag="VIW27_03845"
     CDS             complement(1902..>2835)
                     /locus_tag="VIW27_03845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY5738830.1"
                     /translation="ERLEMGRMPDPECGPGEVEIMVKACGVNFADLLLISGRYQLRPA
                     QPFVPGMEAAGRVTRVGEGVDGISTGDKVAAYVKHGGYADRVVAPMTQVAVLPESISS
                     TAAAAFPVSYASAELAFERASLAAGEVVLIGGAAGAIGTACVELARQRGATVIACVGD
                     REKEELARACGAQHVVSSHSRNLGQDLQAVAPDGIDVIIDPVGGNFFEDSLRALRFGG
                     RLVVLGFASGKISSLRLNHLLVKHQSVIGSSFGLSCIREPAAVAARWPKLVALLEQGQ
                     IKPRVGRTLPFTELPKALNLLKERRVAGRIVLSG"
BASE COUNT          504 a          955 c          870 g          506 t
ORIGIN      
        1 agctggaatg gcgaacatat cggcgccggc aggctgaccc acatcggcct gcgcgcaaat
       61 tccgcgatcg cgcaacagat cgagtttacg cgtccattca aggcgcacca ccgggtcgca
      121 tggtcctttg agagccgcgg cgacaaaacc ctggccagct gggagatgaa cggccgcatg
      181 ccactgctaa agcgcttcat ggccgctcgc atcgagccga tgatcggtcg cgattacgag
      241 cttggcctgg cgttgttgaa cggtttcctg aatgccgacg ccgagcatct acggctgact
      301 ttcgacggcc gtgaacagct cgaggatttc agttactggt cgattccgtg ccacggcaac
      361 ctgcgccagc tcgaggcgaa ccgcgatgcc agcctcgatg ccctgcagcg tgccgccgcc
      421 ggcaagaccg ggctcggcct gacactctac cgcggcttcg atccactggt cgcgcattac
      481 cgcgccgagt tcgcgatacc ggttgtgtcc actacgcccg actcgaacta cacgcggcgc
      541 gagtttcacg gtggcaccta tctgcggatg accttgcgcg gcggccacca gttcctgccg
      601 ctcggctggc atgcgctcta tagccactgc cgcctgttgg gactgaaaac cgaccgtgcg
      661 cgcccgtcac tggagattta ccacaccacg ccggcgcaaa ccgtcgacag caatcaggtg
      721 acgaccgtgt tgtacgcgcc actgaaagcc tgagccgcgg cggcttattc gaaccggcag
      781 ctcggcgtga atgccgacaa ctgcttctcc tcgtcggtca tcacggcctc gatattctcg
      841 aacagcggcg tcgacagata acgttcgccg gtatccggta acatgcacag catattggac
      901 cccgccggcc ccgtctcggc gacctgcagt gcgccggcca gggtgccgcc ggcactgatg
      961 ccggcactga tgccggcgca tatgccttcc tgctgcgcca gctcgcggct gcactccatc
     1021 gcgcaggcgt tgctgatctt gacgatcgcc accacgcagc ggctatccat cgcatcgccg
     1081 gtcagcttag ggatgaaatc gggcgtccag ccctgcctcg ggtgcggcct gaagctcggg
     1141 tggctgtcca cgggtgagcc gtcgtcgtgg cgctgctgcg cgatgccgct gctcagcagg
     1201 ctggcgacct cgggcttgca aacgatgatc cgggtagcgg ggctcttctc ccgtaatacc
     1261 cgcgccacgc ccttgagcgt gccgccggta ccgtaactgg taaccctgta gtcgaggcgc
     1321 acgccgtcga aggcttcgag aatctctggg gccgtggtac gcgaatgcat atcggcattg
     1381 gattcgtttt cgaattgccg ggtcaggaac cagccattct tttcggcgag ttccctggcc
     1441 ttctcgacca tgccgctgcc cttcagcagg gccggcgtca acaccccctg gcgccgagga
     1501 agcgcatcaa ccggcggcgc tcgacgctga aactctcggc catggtgacc accagcggac
     1561 agcccttctg cgcgcagacc atcgccaggc cgataccggt gttgccgctg gtcgcctcga
     1621 taacggtctg gccgggcttc agccggccgg ctttttctgc gtcctcgata atccccagtg
     1681 cgcgccggtc cttgaccgaa ctcatcggat tgaaagcctc gatcttgacg tagaggttga
     1741 cgtgatcggg cgccatagga gtgtttttga agaccggggt attgccgatc gtgccgagaa
     1801 tgttatcgag cacttccact tgcagaccac ttcgatgttc accactgatc ccgcaaccgg
     1861 gataatcggc aagttggggg cagtcccgac cgtcgcggac ctcagccact caacacgatg
     1921 cgacccgcca ctctgcgttc cttgagcaga ttgagcgcct ttggcagctc ggtgaacggc
     1981 agcgtgcggc cgacacgcgg tttgatctgt ccctgttcca gcagggcgac gagtttcggc
     2041 cagcgtgcag ccaccgcagc cggttcgcga atacagctga ggccaaacga tgaaccgatg
     2101 acgctctggt gcttgacgag caggtgattg agcctgagcg acgagatctt gccggacgcg
     2161 aaaccgagca cgaccaggcg cccaccgaac ctcagcgcgc gcagcgagtc ctcgaagaaa
     2221 ttacccccta ccgggtcgat gatgacgtcg ataccgtcag gcgccaccgc ctgcaagtcc
     2281 tggccgagat tgcgcgaatg cgacgagacc acgtgttgcg cgccgcaggc gcgcgccagc
     2341 tcctcctttt ctcgatcacc gacacaggcg atcacggtcg cgccgcgctg cctggcgagc
     2401 tcgacgcagg cggtgccgat agcgccggcc gcgccgccga tcagcaccac ttcgccggcg
     2461 gccagcgagg cacgctcgaa cgccagttcg gcggatgcgt aggagaccgg aaacgcggca
     2521 gcggcagtcg aggagatcga ctccggtagc accgcgacct gggtcatcgg cgcgaccacg
     2581 cggtcggcgt agccgccgtg cttgacgtag gccgcgacct tgtcgccagt gctgattcca
     2641 tcgacgccct cgccgacacg agtcacgcgc ccggcggctt ccatgcccgg cacgaacggc
     2701 tgcgcgggac gtaactggta cctgcccgag atcagcagca gatcggcgaa attgacgccg
     2761 caggccttga ccatgatctc gacctcgccg ggaccacatt cgggatcggg catgcggccc
     2821 atctcgagac gctcg
//
LOCUS       DATCZV010000173         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_225179, whole genome shotgun sequence.
ACCESSION   DATCZV010000173 DATCZV010000000
VERSION     DATCZV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225179"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..2008
                     /gene="trpE"
                     /locus_tag="VIW27_03850"
     CDS             497..2008
                     /gene="trpE"
                     /locus_tag="VIW27_03850"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY5738831.1"
                     /translation="MNPSQFSQLARDFNRVPLVREVFADLDTPLSAYLKLADEPYSYL
                     FESVQGGEKWGRYSIIGMAAGTRIKVFGKRVRVEADGETAVNTEVDDPLRFIEDYLAE
                     FRVAESPCLPKFHGGLVGYFGYDTIRYIEPKLAEGPNPDKIETPDILLMLSDRLVVFD
                     NLSSRMFLIVHVDPSQDDAWQQGQRQLDEFEDRLLSARLERRVKAADQVVGEHDFTSG
                     FTQQGFEEAVEKARQYIIDGDAMQIVLSQRLSIPYSSSPVDLYRALRSLNPSPYLYYL
                     NLDDHHVVGSSPEILVRLEDQTVTVRPIAGTRPRGASAAEDLALEQDLLGDPKELAEH
                     LMLIDLGRNDAGRVSQIGSVRLTEKMIVERYSHVMHIVSNVEGKIRPEMSAFDVLRAT
                     FPAGTVSGAPKIRAMEIIDELEPVKRGIYSGAVGYISWSGNMDTAIAIRTAVIKDNQL
                     YIQAGAGIVYDSVPANEWAETMNKGRAIFRAVALAQSGLPSRDPCSEQSAGDA"
     gene            2008..>2540
                     /locus_tag="VIW27_03855"
     CDS             2008..>2540
                     /locus_tag="VIW27_03855"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5738832.1"
                     /translation="MKVLMIDNYDSFTYNLVQYLGELEAEVEVVRNDQVGIDAIRATD
                     ATHLMVSPGPCTPNEAGISMAAIEALAGRKPILGVCLGHQSIGQVFGGRVVHAHAIMH
                     GKTSPIRHTGAGVFAGLPDPFTATRYHSLVVERDSVPACLEITAWTENDDGSIDEIMG
                     FRHREMPIEGVQFHPES"
BASE COUNT          520 a          770 c          778 g          472 t
ORIGIN      
        1 gctgtcgctg ctgctactgc cgccgcaggc ggcaagaaga aatagctgtg aagcaagcag
       61 cactggcaac cagctacgaa atcgattctt attatcgact ctgttagtca taggaactcc
      121 aacgggtttg tttgtttgaa acgaacatcc tatatagttc gaaattcaaa aaaagtctca
      181 agtttcaata atgtgacgct cgtcccatct gccgattcgg gttgtcgagc agaaaaatgc
      241 atcgatccgg agcgcgcgcg catcgcattc gcgggcggcc gcgtggctcg aagggcaagc
      301 ggggcaaccg cgaaatgatt agtcgtggcc ttgctcgcga gcctgtgata gattctctgg
      361 caggtccgca acaaaatgat tcgcatgact cgaaaacgct tgcagaaagc ccggagtacg
      421 caaaactggc gatggtagat cgcaagccgc tgtacccctg tgccgttttc tgttcaaccg
      481 tttttaagag tcagtcatga atcccagcca attttcgcaa ctcgcccgtg atttcaaccg
      541 cgtgccgctg gtgcgcgaag tcttcgccga tctcgacacg ccgctgtccg cctacctgaa
      601 gctcgccgac gaaccctaca gctacctgtt cgaatcggtg cagggcggcg aaaaatgggg
      661 gcgctactcg atcatcggca tggccgcggg cacgcgcatc aaggtattcg gcaagcgcgt
      721 gcgggtcgag gccgacggcg agacggcggt gaataccgag gtcgatgatc cgctgcgctt
      781 catcgaggat tacctggccg aattccgcgt cgccgagtcg ccctgcctgc ccaagttcca
      841 cggtggcctg gtcggctact tcggttacga cacgatccgc tacatcgaac cgaaactggc
      901 cgagggcccg aatcccgaca agatcgagac cccggatatc ctgctgatgc tgagcgaccg
      961 gctggtggtg ttcgataacc tgtcgtcgcg catgttcctg atcgtacacg tcgacccgtc
     1021 gcaagacgat gcctggcagc aaggccagcg gcaactcgac gagttcgagg accgtctgct
     1081 gagcgcgcgg ctcgagcgtc gcgtaaaggc ggctgaccaa gtcgtcggcg agcatgactt
     1141 cacttccggc ttcacgcagc agggtttcga ggaggcggtc gagaaagcgc gccagtacat
     1201 catcgacggc gacgcgatgc agatcgtgct gtcgcagcgg ctgtcgatac cctactcgtc
     1261 gtcaccggtc gatctgtacc gtgcgctgcg cagcctgaac ccgtcgccct acctgtatta
     1321 cctgaacctc gacgatcacc acgtggtcgg ctcgtcgccg gaaattctgg tgcgcctcga
     1381 ggaccagacc gtgaccgtca ggccgatcgc cggcacccgc ccgcgtggcg ccagcgccgc
     1441 cgaggacctg gcgctggaac aggacctgct tggcgacccg aaggaactcg ccgaacacct
     1501 gatgctgatc gatctcgggc gcaacgatgc cggccgcgtc agtcagatcg gcagcgtgcg
     1561 gctgaccgaa aagatgatcg tcgaacgcta ttcgcacgtg atgcacatcg tctccaacgt
     1621 cgaggggaag attcggcccg aaatgtcggc cttcgacgtg ctgcgcgcca cttttccggc
     1681 cggcaccgtt tccggcgcgc cgaagattcg cgcgatggaa atcatcgacg agctggaacc
     1741 tgtcaagcgc ggcatctatt ccggcgcggt gggctatatc tcgtggagcg gcaacatgga
     1801 taccgcgatc gcgatccgca ccgcggtgat caaggataac cagctgtata ttcaggccgg
     1861 tgccggaatc gtttatgatt cggttccggc aaacgagtgg gcggagacca tgaacaaggg
     1921 gcgggcgata ttccgcgccg tggcgctggc gcagtcgggc ctgccgtcgc gagatccctg
     1981 cagtgagcag tcggcggggg atgcgtgatg aaagtcctga tgatcgacaa ctacgattcg
     2041 tttacctaca acctggtgca atacctcggc gagctcgagg cggaagtcga agtggtgcgc
     2101 aacgatcagg tcggcatcga cgcaatccgc gccaccgacg cgacccacct gatggtctca
     2161 cccgggccct gcacgcccaa cgaggccggc atttcgatgg ccgcgatcga ggcgctggcg
     2221 ggccgcaagc cgattctcgg cgtttgcctc gggcaccaga gcatcggcca ggtattcggc
     2281 ggccgagtgg tgcacgcaca cgcgatcatg cacggcaaaa cctcgccgat caggcacacc
     2341 ggcgcgggag tattcgccgg cctgccggac ccgtttaccg ccacgcgcta tcactcgctg
     2401 gtggtcgagc gcgacagcgt gcccgcgtgt ctggagataa ccgcatggac cgaaaatgac
     2461 gacggcagta tcgacgaaat catgggcttc aggcatcgcg aaatgccgat cgagggggta
     2521 cagtttcatc ccgagtcgat
//
LOCUS       DATCZV010000174         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_595239, whole genome shotgun sequence.
ACCESSION   DATCZV010000174 DATCZV010000000
VERSION     DATCZV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595239"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="VIW27_03860"
     CDS             <1..1850
                     /locus_tag="VIW27_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY5738833.1"
                     /translation="SMRSSIKQVASGRFGVTTEYLVNSDMMQIKVAQGAKPGEGGQLP
                     GHKVDATIAAVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNVNPRGQVSVKLVSE
                     IGVGTVAAGVSKARSDHVTIAGASGGTGASPLTSTMNAGSEWEIGLAETHQTLVLNDL
                     RGRIAVQADGGMRTGRDIVIAALLGADEIGFGTIALIAEGCIMMRKCHLNTCPVGVAT
                     QDPELRKRFTAKPEDLVNFFMFVAEEVREWMARLGYRKFSEMIGHANRLDVNRAIDHW
                     KAKGLDFRGIFSVPQAGPDVAIYNCQEQDHGLDKALDHQLIARLRPALDKGEPVVLED
                     IVVANVNRTVGAMLSGEIARRYGHAGLPDDTIRIKARGNGGQSFGAFLAAGVTLELAG
                     EANDYTGKGLSGGRIIVYPPAESPLKADDNIIVGNTVLYGAISGEVYFSGVAGERFCV
                     RNSGATAVVEGVGDHGCEYMTGGCVVVLGHTGRNFAAGMSGGIAYVLDVDGMFDKRCN
                     LAMVDLEPITDENDALEDLEHQGGDLEAHGLVDVMHDLTRYDELRLKTLIEAHRRYTG
                     SARATEVLENWSDYRGRFVKVMPVEYRKALQKMQQRTQEEAGVSVAVGA"
     gene            1909..>3102
                     /locus_tag="VIW27_03865"
     CDS             1909..>3102
                     /locus_tag="VIW27_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY5738834.1"
                     /translation="MGKPTGFIEISRRDRGYVPVDERVKHYNEFVIPLGEKATREQAA
                     RCMDCGIPFCHNGCPVNNIIPEWNDLVYHDQWREALQVLHSTNNFPEFTGRLCPAPCE
                     ASCTLNLDDEPVTIKTIECAIADRGWQEGWIEPQVPARRSGKRVAVVGSGPAGLACAQ
                     QLGRTGHRVAVFEKNRRMGGLLRYGIPDFKMEADKVDRRIAQMQAEGIRFRPNSHIGV
                     NVHADDLLRDYDAIVLACGSEKPRDLEVPGRQAAGVHFAMDFLTQQNRRVGGEAFGDD
                     EILATDKHVVVIGGGDTGADCVGTSNRQGAASVTQLEIMPRPPERENKLLTWPNWPLR
                     LRTSTSHDEGCERDWSVATRSIKSENGVVTGLELVRLEWQQDETGQWKMSELDGSEFV
                     IPADLM"
BASE COUNT          602 a          997 c         1001 g          502 t
ORIGIN      
        1 attcgatgcg ctcgtcgatc aagcaggtgg cgtcgggacg attcggagtc acgacggaat
       61 acctggtcaa ttccgacatg atgcagatca aggtcgccca gggcgcgaag cccggcgaag
      121 gcggtcagtt gccgggccac aaggtcgacg cgacgattgc cgcggtgcgg cattcgaccc
      181 cgggagtcgg tctgatctcg ccgccgccgc accacgacat ctattcgatc gaggacctgg
      241 cgcagttgat tttcgatctg aagaacgtca atccccgtgg ccaggtcagt gtcaagctgg
      301 tgtcggaaat cggcgtcggc accgttgccg ccggggtatc caaggcgcgt tccgatcacg
      361 tcacgatcgc cggggcgtcg ggcggcaccg gcgccagccc gttgacctcg accatgaacg
      421 caggctcgga gtgggaaatc gggctggccg aaacccacca gacgctggtg ctcaatgacc
      481 tgcgcgggcg catcgcggtg caggccgacg gcggcatgcg caccggccgc gatatcgtca
      541 tcgcggcgct gctcggcgcc gacgagatcg gattcggcac catcgcgctg atcgccgagg
      601 gctgcatcat gatgcgcaag tgccatctca atacctgccc ggtgggcgtc gccacccagg
      661 acccggaact gcgcaagcgc tttaccgcga aacccgaaga cctggtgaat ttcttcatgt
      721 tcgtcgccga agaggtgcgc gaatggatgg cccgcctcgg ttaccgtaaa ttcagcgaaa
      781 tgatcggcca cgccaatcgc ctcgacgtca accgcgcgat cgatcactgg aaggcgaaag
      841 gcctcgactt caggggtatc ttttcggtac cgcaggccgg tcccgacgtc gccatctaca
      901 attgccagga acaggatcac ggcctcgaca aggcgctcga tcaccagctc atcgcccggc
      961 tgcgccctgc gctcgacaag ggcgaacccg tggtactcga ggatatcgtc gtcgccaacg
     1021 tcaaccgcac cgtcggcgcg atgctgtccg gtgaaatcgc gcgccgttac ggtcatgccg
     1081 ggctgccgga cgacaccatt cgaatcaagg cgcggggcaa cggcggccag agtttcgggg
     1141 ctttcctcgc cgcaggcgtt acgctggaac tcgccggtga agccaacgac tacaccggca
     1201 aggggctctc gggcggacgc atcatcgtct acccgccggc cgaatcgccg ctcaaggccg
     1261 acgacaacat catcgtcggc aataccgtgc tttacggcgc gatctccggc gaagtctact
     1321 tctccggggt ggccggcgaa cgtttctgcg tgcgcaattc cggcgccacc gcggtggtcg
     1381 aaggcgtcgg cgatcatggc tgtgagtaca tgaccggcgg ctgtgtggta gttctcgggc
     1441 ataccgggcg caatttcgcc gccggcatga gcggcggtat cgcctacgta ctcgacgtcg
     1501 acggcatgtt cgacaaacgc tgtaacctgg cgatggtcga tctcgaaccg atcaccgacg
     1561 agaacgacgc gctggaggac ctggagcatc agggcggcga tctcgaggcc catggcctgg
     1621 tcgacgtgat gcacgacctg acacgctacg acgagctgcg cctgaagacg ctgatcgagg
     1681 cccaccgccg ttacaccggc agcgccaggg cgaccgaagt cctcgagaac tggagcgact
     1741 accgtggccg cttcgtcaag gtcatgccgg tcgagtaccg caaggcgctg cagaaaatgc
     1801 agcagcggac acaggaagaa gctggcgtca gcgtcgccgt cggcgcctga cgccggcatt
     1861 gttacaccgg gaaccgtggc cgttaggtcc caatcgagag gttgaatcat gggtaagcct
     1921 acaggattta tcgagatttc gcgccgcgac cgtggctatg tcccggtcga cgagcgggtc
     1981 aagcactaca acgagttcgt catcccgctc ggcgagaaag cgacgcgcga gcaggcggct
     2041 cgttgcatgg actgcggcat tccgttttgc cacaacggct gcccggtcaa caacatcatt
     2101 cccgagtgga acgacctcgt ctaccacgac cagtggcgtg aggcactgca ggtgctgcat
     2161 tcgaccaaca actttcccga attcaccggc cgcctgtgcc cggcgccgtg cgaagcctcg
     2221 tgcacgctga acctggacga cgagccggtc accatcaaga ccatcgaatg cgcgatcgcc
     2281 gaccgcggct ggcaggaagg ctggatcgaa ccgcaggtgc cggcgcgccg cagcggcaag
     2341 cgcgtcgccg tggtcggctc cggccccgcg ggcctggcct gcgcccagca actgggacgc
     2401 accggacacc gggtggcggt attcgagaag aatcgccgca tgggcggcct gctgcgctac
     2461 gggattccgg atttcaagat ggaagccgac aaggtcgatc ggcgtatcgc acaaatgcag
     2521 gccgagggaa ttcgttttcg acccaacagc catatcggcg tcaacgtgca cgccgacgac
     2581 ctgttgcgcg actacgacgc gatcgtgctc gcctgcggtt cggaaaaacc gcgcgaccta
     2641 gaggtgcccg gccggcaagc agccggcgtg catttcgcga tggattttct gacccagcag
     2701 aaccgccgcg tcggcggcga ggccttcggc gacgacgaaa ttctcgccac cgacaagcat
     2761 gtcgtcgtta tcggcggcgg cgataccggt gccgactgcg tcggcacctc caatcgccag
     2821 ggtgccgcca gcgtcaccca gctggaaatc atgccgcgcc caccggagcg cgagaacaag
     2881 ctgctaacct ggccgaactg gccgctcagg ctgcgcacct cgacctcgca cgacgagggc
     2941 tgcgagcgcg actggtcggt ggccacgcgc tcgatcaaga gcgagaacgg ggtcgttacc
     3001 ggcctcgaac tggtgcgcct cgagtggcaa caggacgaga ccgggcaatg gaaaatgtcg
     3061 gaactcgacg gcagcgagtt cgtcataccg gcggacctga tg
//
LOCUS       DATCZV010000175         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_330853, whole genome shotgun sequence.
ACCESSION   DATCZV010000175 DATCZV010000000
VERSION     DATCZV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330853"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VIW27_03870"
     CDS             complement(<1..194)
                     /locus_tag="VIW27_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738835.1"
                     /translation="MAVDPENPQINLRVYDFCVRAFRSTRKLLKLNIKLHQDTPGVEH
                     AVHDGDIFLFNHFARFETFIP"
     gene            complement(314..889)
                     /locus_tag="VIW27_03875"
     CDS             complement(314..889)
                     /locus_tag="VIW27_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5738836.1"
                     /translation="MSETPSTMLDIGTPAPDFSLPEPASGKTVGLADFGGQPLLVVFA
                     CNHCPYVLHILDSFTEFANQLQQRGVGVVMISANDITGYPADSPQKMAQLAAEHGFKF
                     PYLYDESQQVALAYRAACTPDFFLFDASHQLVYRGQYDASRPGNQAAVDGGDLRAAVD
                     ALLQGRPVSAEQIPSVGCNIKWRAGNEPDYF"
     gene            1207..1677
                     /locus_tag="VIW27_03880"
     CDS             1207..1677
                     /locus_tag="VIW27_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY5738837.1"
                     /translation="MTTIDLTPLYRSSIGFDRMGSLLDHALRSQKDGSGFPPYDIETT
                     GEDRYAITLAVAGFDESELDIQVENGVLRVRGKKADDSEEKSYLYRGIANRSFERKFN
                     LADHIEITGASLKNGLLTISLVKEIPEAMKPRSIEIATPGATLEHKQKKADKAA"
     gene            complement(1838..1924)
                     /locus_tag="VIW27_03885"
     tRNA            complement(1838..1924)
                     /locus_tag="VIW27_03885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1888..1890),aa:Leu,seq:caa)
     gene            2049..3083
                     /gene="queA"
                     /locus_tag="VIW27_03890"
     CDS             2049..3083
                     /gene="queA"
                     /locus_tag="VIW27_03890"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY5738838.1"
                     /translation="MQLSDFDYHLPQELIAQQPLRERVASRLLLVDPRNGEYADRRFT
                     DFVDLVDPGDLLVFNDTRVIPARLYGEKHSGGRVEVMVERVLDTQRLLAHLRASKAPR
                     PGTSLRLEGEIECVMRARDGDLFVLEQRQGDWLELLERHGHMPLPPYIRRADQRADRE
                     RYQTVYARKPGAVAAPTAGLHFDDGILQRLAAKGVRAAHVTLHVGAGTFQPVRGEDID
                     SHVMHGELLDVPRSVCEAIAETRARGRRVIAIGTTVVRSLESAADNGGVREFHGESNL
                     FIKPGYEFKVVDAMLTNFHLPRSTLLILVSAFAGWQLMRDAYAHAVVEGYRFFSYGDA
                     MFIASRARQA"
BASE COUNT          608 a          957 c         1037 g          585 t
ORIGIN      
        1 ggaatgaagg tttcgaagcg cgcgaagtga ttgaacagaa aaatatcgcc gtcgtgcacg
       61 gcgtgctcga cacccggcgt gtcctggtgc agtttgatgt tcagcttgag cagtttgcgg
      121 gtcgagcgaa acgccctgac gcaaaaatcg tagacccgaa ggttgatttg cggattttcc
      181 ggatcgacag ccattagccg gcgtgtttcg acgatgggtt agacaacgcg gcctggtcca
      241 tgctgcgggc gacccctgga ctgttcaggc ctcgggggcc gtgctcgggg aacagcacat
      301 cgcgcggccg aggctagaag tagtcgggtt cgttgcccgc ccgccacttg atattgcagc
      361 cgacactggg tatctgctcg gccgataccg gcctcccttg aagcagggca tcgaccgcgg
      421 ccctcaggtc accaccgtcg accgcggcct ggttgccggg tcttgaagca tcatactgtc
      481 cgcggtaaac cagctggtgt gaagcgtcga agaggaagaa gtctggcgtg caggcggcac
      541 ggtaagccag cgccacctgt tgcgattcgt cgtacaggta gggaaatttg aaaccatgct
      601 ccgcggccag ctgcgccatc ttttgcgggc tgtcggcagg gtaaccggtg atgtcgttgg
      661 cgctgatcat gaccacgccg acgccgcgct gctgcagctg gttggcaaac tcggtgaacg
      721 agtcgaggat gtgcagcacg taggggcagt ggttgcaggc aaacaccacc agcagcggtt
      781 gtcccccgaa gtccgcgagg ccgacggtct tgccgctggc cggctcggga agcgaaaagt
      841 cgggcgccgg cgttccgata tccagcatcg tcgatggggt ttctgacatg gtctcgctcg
      901 tgttaccgct gtgaacttcg aattataacc cgaatgcgcg cgcgcggtta agcaagcggg
      961 cggaaaccga gggtcaagac cgtcgggcct ggcgtcaacg cggtaccggt ccggtaaagg
     1021 ccgggaagta gccacctgtg tcgccgtccg ccaggcgaag atgaccgttc gagggggcga
     1081 tagcgcgaaa aacaggtatc tcgaggcttg aaattccgct ggtcgttcct atctcgagga
     1141 ctacgccggc aggtctcgag aagaatggcg gcgtggttac taattgcttc tgaaaggaga
     1201 attgttatga cgactatcga tttgaccccc ctgtaccgca gcagcatcgg cttcgaccgt
     1261 atgggttcgc tgctcgatca cgccctgcgc agccagaaag acggctccgg gtttccgccc
     1321 tacgatatcg aaaccacggg cgaagaccgc tacgcgatta ccctcgcggt agccggcttc
     1381 gacgaaagtg agcttgacat ccaggtcgag aacggcgtgt tgcgcgtacg cggcaagaaa
     1441 gccgacgaca gcgaggagaa gtcctacctg tatcgcggta ttgccaatcg cagcttcgag
     1501 cgcaagttca atcttgccga ccatatcgaa attaccggag ccagcctcaa gaatggcctg
     1561 ctgactatca gcctggtcaa ggagattccg gaagcgatga agccgcgctc gatcgaaatc
     1621 gcgactccgg gcgcgaccct cgagcacaag caaaagaaag ccgacaaagc cgcctgataa
     1681 ggccgcttcg agctaacggc gaggccgccg cagccgtccg gttgcggcgg cttttttatg
     1741 tcttcgggac tcacgcacga cggtcgtcgg gggagggcag cgcgcgttgc gaggcgctgt
     1801 tgtggcgaat ctgcacgcag atctggatcc aaaagtctgg taccgggagg gggactcgaa
     1861 cccccaaggt gtcaccacca gcggattttg agtccgccgc gtctaccagt tccgccatcc
     1921 cggcaagggt gggcgaattc taattgaatt cagagcggag tggtagcttg tgcggcgcgc
     1981 gctcgaaaac cacagtggcg agttcggcaa atggcgctat catacgcgct tttcgggcca
     2041 ttcccggcat gcagctcagc gatttcgatt accacttgcc gcaggaactg attgcacagc
     2101 agccgttgcg cgagcgcgtc gcctcgcgcc tgttgctggt cgatccgcgc aacggcgagt
     2161 acgccgatcg ccggttcacc gatttcgtcg atctcgtcga tcccggagac ctgttggtgt
     2221 tcaacgatac gcgggtgatc ccggcccgcc tgtacggcga gaaacacagc ggcggccggg
     2281 tcgaagtgat ggtcgaacgg gtgctcgata cgcagcggct gctcgcccac ctgcgcgcca
     2341 gcaaggcacc gcggccgggc acgtcgctca ggctggaagg tgaaatcgag tgcgtgatgc
     2401 gcgcccgcga cggcgatctt ttcgtgctcg agcagcggca gggcgactgg ctggaattgc
     2461 tcgagcgtca cgggcacatg ccgttgccgc cgtacattcg ccgcgccgac cagcgtgccg
     2521 accgcgaacg ctaccagacg gtctatgccc gcaaacccgg cgcggtcgcg gcgccgaccg
     2581 cgggactgca cttcgacgac ggcatcctgc agcggctggc ggcaaagggc gtgcgcgcgg
     2641 cccatgtcac gctgcatgtc ggcgccggca ccttccagcc ggtacgtggt gaagatatcg
     2701 actcccacgt gatgcacggc gaactgctcg atgtgccgcg ctcggtctgc gaggcgatcg
     2761 cggaaacccg tgcgcgcggg cggcgagtca tcgccatcgg caccaccgtg gttcgtagcc
     2821 tcgagtcggc cgccgacaac ggtggtgtgc gcgagtttca cggcgagtcg aacctgttca
     2881 tcaagccggg ctacgaattc aaggtcgtcg atgcgatgct gaccaatttc cacctgccgc
     2941 gctcgacgct gttaatactg gtcagcgcgt tcgccggctg gcaattgatg cgcgacgcct
     3001 acgcgcacgc ggtcgtcgag ggttatcgat tcttcagtta cggcgacgcg atgttcatcg
     3061 ccagccgcgc acggcaggcc tgacccatgc aattcgacct cgcaactacc gatggcgacg
     3121 cgcgacgcgg gcgcctgcgg ttcgaccgcg gcagtgtcga gacgccagcc ttcatgccgg
     3181 tggggac
//
LOCUS       DATCZV010000176         7566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_383550, whole genome shotgun sequence.
ACCESSION   DATCZV010000176 DATCZV010000000
VERSION     DATCZV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383550"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="rapZ"
                     /locus_tag="VIW27_03895"
     CDS             complement(<1..836)
                     /gene="rapZ"
                     /locus_tag="VIW27_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY5738839.1"
                     /translation="MTSDKDASQHENELLKLVIVSGLSGSGKTVALRTLEDGGYYCID
                     NLPLGMLPELIDNMINTRFAPLESVAIAIDARSGVEHAQRFDEIVRQVTAHTIQLKIV
                     FLSCETSRLLKRFSETRRKHPLSREGLPLADAIQQERKLLADIQANANLTIDSTNLNV
                     HQLRQTILDRLLSNSAAAMDVLIQSFGFKNGVPADTDFVFDVRCLPNPHWEKELRPLT
                     GRDRAVIKYLESYPEVDEMATTIHQFLALWIPRFRKENRSYLTVSLGCTGGQHRSVYL
                     AER"
     gene            complement(912..1364)
                     /locus_tag="VIW27_03900"
     CDS             complement(912..1364)
                     /locus_tag="VIW27_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY5738840.1"
                     /translation="MKISALLSPKSIFLDVEVTSKKKLLELIANIVAGRTELSESTIF
                     GNLLNRERLGSTALGRGFAVPHARVDNLDDTLACFFRLKEAVNFEAPDDVPVDLVFTI
                     VIPEQATEEHLIILSSLARIFSSEEVCDAIREAGSKDEIAQIIESAED"
     gene            complement(1491..1808)
                     /gene="raiA"
                     /locus_tag="VIW27_03905"
     CDS             complement(1491..1808)
                     /gene="raiA"
                     /locus_tag="VIW27_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY5738841.1"
                     /translation="MQVSLSGHHVDITDSMRNYVNEKIARLDRHFDQALDIHIVLTVE
                     KLRHKAEATLHVSGGNLHADDVRDDMYAAIDGLIDKLDRQGKKHKEKLKDHRLKNIPV
                     ESV"
     gene            complement(1859..3352)
                     /locus_tag="VIW27_03910"
     CDS             complement(1859..3352)
                     /locus_tag="VIW27_03910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY5738842.1"
                     /translation="MKQKLQLRIGQSLQMTPQLQQAIKLLQLSSLELQTEIQQLLDSN
                     PLLDQEDNLGESTLADFDTQRETNSNLEDTTAYTSSAANVAENRAEQRLPDELEIDAR
                     WEQIYDQSPGQGSGSQASAIDHREMFENQSGEVDTLKQHLLWQLDCSRLSPTDYDIGL
                     AVIDSINDNGYLGQSLEDIYEGLQNQFENLEFDEVEAILHLVQHFDPVGVAARSPSEC
                     MLIQLSQYDPETPHLAKARLLVENYLEHLAGNDMALLRRRLQAGHAELEQIIRLIQTT
                     NPHPGHSVSQNHAEYIVPDVYVSRKSGVWQASINPDNAPRLRVNDRYAGMIKRSDSSD
                     QNTYLKDHLQEARWFIKSLQSRNDTLLRVAEAIVERQHAFLDYGEEAMQPMVLRDIAE
                     QLEMHESTISRVTTQKYMHTPRGILEFKYFFSSHVDTADGGECSATAIRAIIKKFVAA
                     ERPEKPLSDNKIASLLGEQGIKVARRTVAKYREAMHIPPSNERKRLF"
     gene            complement(3414..4148)
                     /gene="lptB"
                     /locus_tag="VIW27_03915"
     CDS             complement(3414..4148)
                     /gene="lptB"
                     /locus_tag="VIW27_03915"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY5738843.1"
                     /translation="MNGSHLLEASELAKSYKRREVVKSVSLRVSGGEIVGLLGPNGAG
                     KTTCLYMVVGLIDNDSGVISLNGRPIDHLPMHARARLGIGYLPQEASVFRNLTVEQNI
                     LAILQLRRDLDRPGRRRLLQQLLEEFQIEYLRASEGISLSGGERRRVEIARALANNPE
                     FILLDEPFAGVDPISVIEIQTIVRQLAERGIGVLITDHNVRETLGICDRAYVLGEGRI
                     LAEGDPGQILENKMVRKIYLGDNFKL"
     gene            complement(4154..4672)
                     /gene="lptA"
                     /locus_tag="VIW27_03920"
     CDS             complement(4154..4672)
                     /gene="lptA"
                     /locus_tag="VIW27_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY5738844.1"
                     /translation="MTAANPLSTIIALALLLLPAAAPRALSTDGEQPILVEADSLEMR
                     DAENISIYEGNVSLVQGSLKLNSERLVIHFNDDNELVLIVMTGSPARFRQLDDQRREM
                     LGQAMRIRYLEADSMLELYESASFTHAGDHIESDRIHVNTRDNSIEAGGKQSDQRVKM
                     LIQPRQDNGTTE"
     gene            complement(4659..5216)
                     /gene="lptC"
                     /locus_tag="VIW27_03925"
     CDS             complement(4659..5216)
                     /gene="lptC"
                     /locus_tag="VIW27_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY5738845.1"
                     /translation="MKSRLTILIVVLGIGAVLLGWVYESQLRTRVETRELVVPDNIDY
                     FLANLKFRAIDDHGKLEYALQSPRLEHFPRGDLSRIETPALQIYSPRDDWQVVALEGE
                     FRHADNLLYLRRDVVMNREGVDPLRLSTESIRFEPARDFVTTEAEVVIETHAGRIEAE
                     RASFDLAAGIYRFTRSSAVYHNDGS"
     gene            complement(5213..5740)
                     /gene="kdsC"
                     /locus_tag="VIW27_03930"
     CDS             complement(5213..5740)
                     /gene="kdsC"
                     /locus_tag="VIW27_03930"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HEY5738846.1"
                     /translation="MTAENGARARQVKLLIVDVDGVLTDGGLYFDNRGEELKRFCSLD
                     GHGMRMALGGGIEIAVITGRRSNIVEHRMRDLGVSHVYQGSRDKLPVFEQLLRDTGLE
                     AEQAAYVGDDLPDLPIMHRVGFAIAVQNAHGFVKQHCDWVTSARGGDGAVREVTDFIL
                     HSQGLLDAQQQGYLT"
     gene            complement(5917..6891)
                     /locus_tag="VIW27_03935"
     CDS             complement(5917..6891)
                     /locus_tag="VIW27_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY5738847.1"
                     /translation="MTRHAFLELGLAVLKTEAEGINEVSGRLDQSFTDACELILRCSG
                     RIVVTGMGKSGHIGNKIAATLASTGTPAFFMHSGEASHGDLGMITEQDLVIALSNSGE
                     TSEITLLLPLLKRLGIAVIALTGNPASTLARSAEIHLDVSVSKEACPLGLAPTSSTTA
                     SLAMGDALAIAVLEARGFTEEDFALSHPGGNLGRRLLLRVSDIMHTGTAIPLVPVGSS
                     LKDTLIEMTAKGLGFAGVTEADGQRLVGIYTDGDLRRTFETMPDIGSSRVEDFMTASC
                     VTIDADRIASEALKIMHDRKINALMVVGDDARVQGALNMHDLLRAGVV"
     gene            complement(7138..>7566)
                     /gene="fabA"
                     /locus_tag="VIW27_03940"
     CDS             complement(7138..>7566)
                     /gene="fabA"
                     /locus_tag="VIW27_03940"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEY5738848.1"
                     /translation="LPPMLMFDRIIRITEEGGKFDKGEIIAELDVDPELWFFKCHFQT
                     DPVMPGCLGLDAMWQLIGFFLGWKGGPGHGRALGAGEVKYFGQVLPDAKLVTYRIDMK
                     RVIMRKLVMGIGDASMEVDGREIYSARDLRVGLFTSTDDF"
BASE COUNT         1327 a         2249 c         2374 g         1616 t
ORIGIN      
        1 cgttccgcga gatagaccga gcggtgctgg ccgccggtgc agccgaggga aacggtcaga
       61 tagctgcggt tttcctttct aaaccgcgga atccacagcg ccaggaattg atgaatcgta
      121 gtcgccatct cgtcgacctc gggataggat tcgaggtact tgattaccgc ccgatcgcgg
      181 ccggtcagcg gccgcaattc cttttcccag tgcgggtttg gcaggcaacg gacgtcgaac
      241 acgaaatcgg tatcggccgg cacgccgttc ttgaaaccga aggattgaat cagcacgtcc
      301 attgccgccg ccgaattcga cagcagccgg tcgagtatcg tctgcctgag ctggtgtacg
      361 ttcaggttgg tgctgtcgat ggtcaggttg gcgttggcct ggatatccgc cagcagcttg
      421 cgctcctgct gtatcgcgtc cgccagcggc aggccctcgc gggacagcgg gtgcttgcgg
      481 cgcgtctcgc tgaagcgttt cagcaaccgc gaggtctcgc aactcaggaa gacaattttg
      541 agttgtatcg tatgcgcggt cacctggcgt acgatttcgt cgaagcgctg cgcatgttcg
      601 acgccgctgc gggcatcgat ggcgatggcc accgactcca gcggcgcgaa gcgcgtattg
      661 atcatgttgt cgatgagttc cggcagcatg ccgagcggca ggttgtcgat gcagtaataa
      721 ccgccgtctt cgagcgtgcg cagggcgacc gtcttgccgg agcccgacag cccgctgacg
      781 atcaccagct tcagcagttc gttttcgtgt tgtgaagcgt ccttatcaga agtcatgagg
      841 tgattaccgg tgcaaacgaa ccgcggcagc cggcggtcgg ccggcgatcg ccgctgctgg
      901 caggccgcga actagtcttc ggccgattcg atgatttgcg cgatctcatc cttgctgccg
      961 gcctcgcgaa tcgcatcgca aacctcttcg ctggagaata tcctggccag tgacgacagg
     1021 atgatcaggt gctcctcggt cgcctgttcc ggaattacga tggtgaacac caggtccacc
     1081 ggaacatcgt cgggtgcttc gaagttgacc gcctccttga ggcgaaaaaa acaggccagc
     1141 gtgtcgtcga ggttgtcgac gcgggcgtgc ggtaccgcga atcctctgcc cagcgcggtg
     1201 ctgccgaggc gttcgcggtt cagcaggttg ccgaaaatcg tcgactcgga cagctccgtg
     1261 cgccccgcga cgatattggc gatcagttcc aggagtttct ttttgctggt cacctcgacg
     1321 tcgagaaaga tacttttggg cgacagcagc gctgagattt tcattgcgac ctgggtcggg
     1381 aatgccgagt attcgaagtt gggcgaatgt tagtcaaatc gccgcagtat gcaagcccgg
     1441 ccggcttgcc ggcgtcgagg ctcgggggag ccgcggctcc cccggcagac tcagaccgat
     1501 tccacgggga tattcttgag gcgatgatcc ttgagcttct ccttgtgctt tttgccctgg
     1561 cgatccagtt tgtcgatcag gccgtcgatc gccgcataca tgtcgtcccg aacatcatca
     1621 gcgtgcaggt tgccgccgct aacgtgcagc gtggcctcag ccttgtgacg cagtttttcc
     1681 acggtcaata cgatatgtat gtccagcgcc tggtcgaaat gccggtccag ccgggcaatt
     1741 ttttcattga cgtagttgcg catggaatcc gtgatgtcga catgatggcc gcttaaacta
     1801 acttgcatct gtaacctcct cggctgtgca ttccgcatca ttgcggatga ttaatgtttc
     1861 aaaaaagacg tttacgttca ttggacggcg gaatatgcat cgcctcccgg tacttggcca
     1921 ccgttctgcg ggcgaccttg attccctgtt cgcccagcaa actggcgatc ttgttgtcgc
     1981 tgagaggctt ttccggccgc tcggcggcga cgaatttctt gatgatcgca cgaatcgcgg
     2041 tggccgaaca ttctccaccg tcggcggtat cgacgtggct ggagaagaaa tacttgaatt
     2101 cgagaatgcc gcgcggcgtg tgcatgtatt tctgcgtggt aacccgcgag atggtcgatt
     2161 cgtgcatttc gagctgctcg gcgatatcgc gcaacaccat cggctgcatc gcttcctcgc
     2221 cataatcgag gaacgcatgc tggcgttcga cgatggcctc ggcgacgcgt aacagggtgt
     2281 cgttgcgact ttgcaggctt ttgatgaacc agcgcgcttc ctgcaggtgg tccttgagat
     2341 aggtattctg gtcgctgctg tcgctgcgct tgatcatccc ggcatagcgg tcgttgacgc
     2401 gcagccgggg tgcattgtcg ggattgatac tggcctgcca gacgccggat ttcctgctga
     2461 cgtagacgtc cggcacgatg tactcggcat ggttttgact gacgctgtgc ccggggtgcg
     2521 gattggtggt ctgaatcagc ctgatgatct gttcgagctc cgcatggccg gcttgcaggc
     2581 gccggcgcag cagtgccatg tcattgccgg cgagatgctc gaggtagttt tccaccagca
     2641 ggcgggcctt ggcgagatgc ggagtctcgg gatcgtactg gctcagctgg atcagcatgc
     2701 attcgctcgg gctgcgcgcc gccaccccga ccgggtcgaa gtgctgcacc aggtgcaaga
     2761 tggcctcgac ctcgtcgaat tcgaggttct cgaactggtt ttgcaagccc tcgtagatgt
     2821 cttccagcga ttgccccagg taaccgttgt cgttgatcga atcgatcact gccagcccga
     2881 tatcgtaatc ggtcggcgac aggcgcgagc aatccagctg ccagagcagg tgctgtttga
     2941 gggtatcgac ctcaccgctc tggttttcga acatttcgcg gtggtcgatc gccgatgcct
     3001 gtgagccact gccttgcccc ggggactgat cgtagatctg ctcccagcgg gcgtcgattt
     3061 cgagttcgtc gggcaggcgt tgctcggcgc ggttttcggc cacgttcgcc gctgacgagg
     3121 tgtaggcggt ggtgtcctcg aggttggaat ttgtctcgcg ttgcgtgtcg aaatcggcga
     3181 gcgtgctttc gcccagatta tcttcctggt cgagcagcgg attggagtct agcagttgct
     3241 gaatttcggt ttgcagctcc agcgaggaga gttgcagcaa cttgatcgcc tgctgcaatt
     3301 gcggcgtcat ctgcaggctt tgccctatgc gcagttgcag cttttgtttc attgtcgatg
     3361 cgagtccgtc gcgcgatttc cgtgaggatt caggtggtct cccatgcagc tagttatagc
     3421 ttaaaattgt ctcccaagta aatctttcgg accattttat tttccaatat ctgacccgga
     3481 tcaccctcgg caagaatgcg gccttcgccc agtacgtagg cacggtcgca tatgcccagg
     3541 gtctcgcgta cgttgtgatc ggtgatcagc acgccgatgc cgcgctcggc gagctggcgg
     3601 acgatcgtct ggatttcgat caccgagatc gggtcgaccc cggcgaacgg ctcgtcgagc
     3661 aggatgaatt cgggattgtt ggcgagcgcg cgcgcgatct ccacccggcg gcgctcgccg
     3721 cccgacagcg agatgccctc gctggcgcgc aggtactcga tctggaattc ctcgagcaac
     3781 tgctgcagca gcctgcggcg cccaggccgg tcgagatcgc ggcgcagctg caggatcgcg
     3841 agaatattct gctccacggt caggttgcga aaaaccgagg cctcctgcgg caggtagccg
     3901 atgccgaggc gcgcgcgcgc gtgcatcggc aggtggtcga tcggcctgcc attcagcgag
     3961 atcacgcccg agtcgttgtc gatcagcccg accaccatat ataagcaggt ggtcttgccg
     4021 gcgccgttgg ggcccagcag gccgacgatc tcgccgccgg agacccgtaa agaaaccgat
     4081 ttgaccacct cgcgccgctt gtaggacttg gccagctcgc tggcctcgag caggtggctg
     4141 ccgttcatcg cggctattcc gtggtgccgt tatcttgccg cggctgaatc agcatcttca
     4201 cgcgttgatc ggattgtttg ccaccggcct caatgctgtt gtcgcgggta ttgacatgaa
     4261 tgcggtcgct ttcgatgtgg tccccggcat gggtgaagct cgcggattca tagagttcca
     4321 gcatcgagtc ggcttccagg tacctgatgc gcatggcctg acctagcatt tcccggcgct
     4381 gatcgtcgag ctgccggaag cgggccggcg agccggtcat tacgatcagc accagttcgt
     4441 tgtcgtcatt gaaatgaatc accaggcgct cggaatttaa tttcaggctg ccctgcacca
     4501 ggctgacgtt gccctcgtag atactgatgt tttcggcatc gcgcatctcg aggctgtcgg
     4561 cctccaccag gatcggctgc tcgccgtcgg tggatagcgc gcgcggtgcg gccgccggca
     4621 atagcagcag cgccagcgcg atgatcgtgg aaagcgggtt agctgccgtc attgtggtag
     4681 accgcgctgg agcgagtgaa gcggtagatg ccggcggcga gatcgaaact ggcccgctcg
     4741 gcctcgatgc gcccggcatg ggtttcgatg acgacctcgg cctcggtggt gacgaaatcc
     4801 cgtgcgggct cgaaacgaat gctctcggtc gacaggcgca acggatcgac cccttcgcgg
     4861 ttcatgacca cgtcgcgccg caggtacaac aggttgtcgg catgccggaa ttcgccctcc
     4921 agcgcgacca cttgccagtc gtcgcgcggg ctatagattt gcagcgccgg ggtctcgata
     4981 cggctcaggt cgccgcgcgg aaaatgctcc aggcgcgggc tctgcaatgc gtattcgagc
     5041 ttgccgtggt cgtcgatggc gcggaatttc aggttggcga gaaagtagtc gatgttgtcg
     5101 gggaccacca gttcgcgggt ttcgacccgc gtgcgcagtt gcgactcgta tacccagccg
     5161 agcagcaccg cgccgattcc cagcaccacg atcagaatcg tcagccggga cttcatgtca
     5221 ggtaaccttg ctgctgcgcg tcgagcaggc cttgcgaatg cagaatgaaa tccgtgacct
     5281 cgcgcaccgc gccgtcgccg ccgcgtgcgc tggttaccca gtcgcagtgc tgcttgacga
     5341 aaccgtgggc gttttgcacc gcaatcgcga agccgacgcg gtgcatgatc ggcaggtccg
     5401 gcaggtcgtc gccgacatag gctgcctgct cggcctcgag accggtgtcg cgcagcagtt
     5461 gctcgaacac cggcagcttg tcgcgcgagc cctggtagac atggctgacg ccgaggtcgc
     5521 gcatgcgatg ctcgacgatg ttcgagcggc ggccggtaat caccgcgatt tctatgccgc
     5581 cgcccagtgc catgcgcatg ccatggccgt cgagggagca gaagcgcttc agttcctcgc
     5641 cacggttgtc gaaatacagg ccgccatcgg tcagtacgcc gtcgacatcg acgatcagca
     5701 atttcacctg cctggcgcgg gcgccattct ccgcagtcat gattgccgcc gagccgcggg
     5761 ttgtgccagc gaacggtggg ccggcacggg gctgcagcga ccggcagccg ggccgccgtc
     5821 gccgcgagtt ttcaagattc tatttccgtg cattcaaata ttttccacat tcgtcggtcc
     5881 gcggccagcc cggggacaac cattgtaaca ggcgggctag actacacccg ctcttaatag
     5941 atcatgcatg tttaacgcac cctgaacgcg ggcatcgtcg ccgaccacca tcagggcgtt
     6001 gattttcctg tcgtgcatga ttttcagcgc ctcgctggcg atgcgatcgg cgtcgatggt
     6061 cacgcagctc gcggtcatga agtcctcgac gcgcgaggag ccgatatcgg gcatcgtttc
     6121 aaaggtgcgc cgcagatcgc catcggtata aataccgacc aggcgctgtc cgtcggcctc
     6181 ggtcacgccg gcaaagccga gtcccttggc agtcatttcg atcagcgtat ccttcagcga
     6241 gctgccgact ggcaccagcg gaatcgcggt accggtatgc atgatgtcgc tgacgcgcag
     6301 cagcaggcgt ctgccgaggt tgccgcccgg gtgggagagc gcgaaatctt cctcggtgaa
     6361 accgcgagcc tcgagcaccg cgatcgccag cgcatcgccc attgccaggc tcgcggtggt
     6421 gctggaagtc ggtgccagtc ccagcgggca ggcttccttg ctgacgctga cgtcgaggtg
     6481 gatttcggcg ctgcgggcca gcgtcgaggc cgggttgccg gtgagcgcga tcaccgcgat
     6541 gccgaggcgc ttcaacagcg gcaaaagcag cgtgatttcg ctggtctcgc cggagttgga
     6601 caacgcgatt accaggtcct gctcggtaat catgccgaga tcgccgtggc tggcttcgcc
     6661 ggaatgcatg aaaaacgccg gcgtgccggt gctcgccagg gtagccgcaa tcttgttgcc
     6721 gatatgcccc gacttaccca tgccggtgac gacgatgcgc ccgctgcagc gcaggatcaa
     6781 ttcgcaggcg tcggtgaaac tctgatcgag gcgcccgctc acctcgttaa tgccctcggc
     6841 ttcggttttg aggaccgcga gacccagctc caggaacgcg tgtctggtca tcgcgggccg
     6901 agcgggtatt ccccggtgtg gttgcagtgc acgactatag tctcccggtg attgtcgggt
     6961 tgccagcgat taccgtggtc gccgctaaac aggttcagga cccggttacc gtgctggcgc
     7021 atccggcggg ctgttcgacg acggcctcat gatacaaata tcgctgcgaa atgacaaaat
     7081 tcccgcgtgc ggaagtcggc gcctgcggct ggtcgcggca cccggcggtt gcggatctca
     7141 gaaatcgtcg gtagaggtga acaggccgac acgcagatcg cgagcgctgt agatctcgcg
     7201 gccgtcgacc tccatgctcg cgtcgccgat acccatcacc agcttgcgca tgatcacgcg
     7261 tttcatatcg atgcggtaag tgaccagttt tgcatcgggc agcacctggc cgaagtactt
     7321 gacctcgccg gcgccgagcg cgcggccgtg cccggggccg cccttccagc cgaggaaaaa
     7381 gccgatcagc tgccacatcg cgtcgagccc caggcacccc ggcatcaccg ggtcggtctg
     7441 gaaatggcac ttgaaaaacc acagctcggg atcgacgtcg agttcggcga tgatttcgcc
     7501 cttgtcgaat ttaccgcctt cctcggtgat gcgaatgatg cgatcgaaca tcagcatcgg
     7561 cggcag
//
LOCUS       DATCZV010000177         3906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_172478, whole genome shotgun sequence.
ACCESSION   DATCZV010000177 DATCZV010000000
VERSION     DATCZV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172478"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /gene="acnA"
                     /locus_tag="VIW27_03945"
     CDS             complement(<1..2526)
                     /gene="acnA"
                     /locus_tag="VIW27_03945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5738849.1"
                     /translation="MTPGNNSFDTLSKLTVGDRSYLYYSLEKLTGEKPAIAKLPYSLK
                     VLLENILRYEQGGEDSAGDVAAFEEWVENRESRREIAFRPARVLMQDLTGVPAVVDLA
                     AMRDAADDPQRINPQIPIDLVIDHSVMVDEFASPGAFAANVKLEVKRNDERYRFLKWG
                     AGAFDNFRVVPPGTGICHQVNLEYLAKTVWQQQVGDETYVFPDTLVGTDSHTTMINGL
                     GVLGWGVGGIEAEAGMLGQPISLLIPQVVGFQFTGQLREGITATDLVLTVTQMLRQRG
                     VVGKFVEFCGPGLDALPLADRATIANMAPEYGATCGFFPVDAETLRYLELTGRDRQTL
                     ELVEAYCRAQGMWYQPDTPDPVFSNTLTLDLSSVEASLAGPKRPQDRVALSGIKAATE
                     EIVGDSASGERGSDDELRDGDVVIAAVTSCTNTSNPKVMMAAGLVARKARALGLGTRP
                     WVKTSLAPGSKVVTEYLTASGLQEDLDAIGFNLVGYGCTTCIGNSGPLPPAIEKQLRE
                     RKLTVASVLSGNRNFEGRIHQLVRANWLASPPLVVAYALAGSTRIDLTSEPLGESADG
                     EPVYLRDIWPSNQEIADEVIKVNEEMFNRQYADVFSGDDNWRAIEVEQNMTYPWDDAS
                     TYIRNPPYFSLDNSRQLEPIMHARILAIFGDSITTDHISPAGAIAVDSPAGKYLIERG
                     VERSGFNSYGSRRGNHELMMRGTFANIRIRNEMTPEVEGGVTRLMPGGEQMSIYDAAM
                     KYQANSTPLVVIAGKEYGTGSSRDWAAKGTRLLGVKAVIVESFERIHRSNLVGMGVLP
                     LQFKDGLDRKQLGLDGTEVIDITGVAGGITPRMDVNCRITYADG"
     gene            complement(2568..3473)
                     /locus_tag="VIW27_03950"
     CDS             complement(2568..3473)
                     /locus_tag="VIW27_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5738850.1"
                     /translation="MARDTINCVVMFADVAGSTSMYENMGDELARERISKALDALISI
                     SGSHDGKLVKTIGDEILVYFIDADAAVNAAREIQETMEDHRTPETVGVSIRIGMQYGS
                     IILEKDDIFGDTVNVAARIASMAKARQILCTQEIAEMVRNPELSSNMRPYDRLRVKGR
                     NEQLDVYLYGWEQEGDITNMATLSSFTNPARFSQATKLMLRYEGSALSIDADSDVYIL
                     GRGKDCELVVTGDLISRYHSRIEHRRGKFVIADQSTNGTFVRTVDGQDVYLRREEYTL
                     FGSGFISLGKKIDLNDDNLIHFQCE"
     gene            complement(3666..>3906)
                     /locus_tag="VIW27_03955"
     CDS             complement(3666..>3906)
                     /locus_tag="VIW27_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY5738851.1"
                     /translation="RERLAALESWNAAAIHAAIEQLTGELGVGMGKIGQPLRVAVTGG
                     SFSPPIDQTLELLGRQTSLARVDRAIEFVSTARAD"
BASE COUNT          690 a         1218 c         1199 g          799 t
ORIGIN      
        1 gccatcggcg taggtgatcc tgcagttgac gtccatacgc ggcgtgatgc cgccggcgac
       61 gccggtgata tcgatcacct cggtgccgtc cagccccagc tgcttgcggt cgagtccgtc
      121 cttgaactgc aacggcagca cgcccatgcc gaccaggttc gaccggtgga tgcgctcgaa
      181 actctcgacg atcaccgcct tgacgccgag caggcgtgtg cccttagcgg cccagtcgcg
      241 gctcgaaccg gtgccgtatt ccttgccggc gatgaccacc agcggcgtgc tattcgcctg
      301 gtatttcatc gccgcgtcgt agatgctcat ctgctcgccg cccggcatca ggcgggtgac
      361 gccgccctcg acctccgggg tcatctcgtt gcgaatgcga atgttggcga aggtgccgcg
      421 catcatcagt tcgtggttgc cgcgccgtga gccgtaggaa ttgaacccgc tgcgctcgac
      481 gccgcgctcg atcaggtact tgccggccgg gctgtcgacc gcgatcgcgc cggccggcga
      541 gatatggtcg gtggtgatcg aatcgccgaa aatcgccagg atgcgggcgt gcatgatcgg
      601 ttccagttgc cggctgttgt cgaggctgaa gtacggcggg ttgcgaatat aggtcgaggc
      661 atcgtcccag gggtaagtca tgttctgctc gacctcgatg gcgcgccagt tgtcgtcgcc
      721 gctgaacacg tcggcgtact ggcggttgaa catttcctcg ttgaccttga tcacctcgtc
      781 ggcgatctcc tggttgctcg gccagatatc gcgcaggtat accggctcgc cgtcggcaga
      841 ttcgccgagt ggttcggagg tcaggtcgat gcgggtactg ccggccagtg cataggcgac
      901 caccagcggc ggcgatgcca gccagttcgc cctcaccagc tggtgaatac gcccctcgaa
      961 gttgcggtta ccggacagca cgctggcgac ggtcagctta cgctcacgca gctgcttctc
     1021 gatcgccggc ggcaggggac cggaattgcc gatacaggtg gtgcagccgt agcccaccag
     1081 gttgaagccg atggcgtcga gatcctcctg caagccgctc gccgtcaggt actcggtgac
     1141 gactttcgag ccgggcgcga gcgaggtctt tacccatggc ctggtgccga gacccagcgc
     1201 cctggctttg cgcgcaacca gtccggcggc catcatcacc ttgggattcg aggtgttggt
     1261 gcacgaggtg accgcggcga taaccacgtc gccgtcgcgc aattcgtcat cgctgccgcg
     1321 ctcgccgctc gccgagtcgc cgactatttc ctcggtcgcc gccttgatgc cggacagcgc
     1381 gacgcgatcc tgcgggcgtt tcggtccggc cagagaggct tcgacgctcg acagatccag
     1441 cgtcagcgta ttgctgaaaa ccgggtccgg ggtgtcgggc tgataccaca tgccctgcgc
     1501 ccggcaataa gcctcgacga gttcgagcgt ctgccggtcg cggcccgtca gctccaggta
     1561 gcgcagggtt tccgcgtcga ccgggaaaaa tccgcaggtg gcgccatact cgggcgccat
     1621 gttggcgatg gtggcgcggt cggccagcgg cagggcgtcg agtcccggcc cgcagaactc
     1681 gacgaacttg ccgaccaccc ctcgctgccg cagcatctgg gtcacggtca gcaccaggtc
     1741 ggtggcggtg atgccctcgc gcaactggcc ggtaaactga aagccgacga cttgtggaat
     1801 caacagcgaa atcggctggc cgagcatgcc cgcctcggcc tcgatgccgc cgacacccca
     1861 gccgagcacg ccgaggccgt tgatcatcgt ggtatgcgaa tcggtgccga ccagggtgtc
     1921 cggaaacaca taggtctcgt cgccgacctg ctgctgccat accgtcttcg ccaggtactc
     1981 gagattgacc tggtggcaga taccggtgcc gggcggcacc acgcgaaaat tatcgaaggc
     2041 gccggcgccc catttgagaa accggtaacg ctcgtcgttg cgctttactt ccagcttcac
     2101 gttggcggca aacgccccgg gagacgcgaa ttcgtcgacc atcaccgagt ggtcgatcac
     2161 caggtcgatc ggaatttgcg gattgatacg ctgcggatca tcggccgcgt cgcgcatcgc
     2221 cgcgagatca accaccgccg gcacgcctgt caggtcctgc atcaacacgc gcgccggccg
     2281 aaacgcgatt tcgcgccgcg actcgcggtt ttctacccac tcctcgaagg ccgcgacatc
     2341 accagcgctg tcttcgccgc cctgctcgta gcgcaggata ttctcgagca aaaccttcag
     2401 cgaatacggc agtttcgcga tcgccggttt ttcgccggtg agcttttcga ggctgtagta
     2461 aagataactg cggtcaccga cggtgagttt tgatagcgtg tcgaaactgt tgtttccagg
     2521 tgtcatgggg aatctccgtc ctgctgctcg gcgaagatta taacgcgtta ctcacactgg
     2581 aaatgaatta gattgtcgtc attgaggtcg attttcttgc cgagactgat gaacccggat
     2641 ccgaacaggg tgtattcctc gcggcgcagg taaacatcct gcccgtcgac ggtgcgcacg
     2701 aaggtgccgt tggtgctctg atcggcgatc acgaatttac cgcgccggtg ttcgatgcgc
     2761 gaatggtagc gggagatcag gtctcccgtg acgaccagtt cgcaatcctt gccacggccg
     2821 agaatgtaga cgtccgaatc ggcatcgatc gacagcgcgc tgccttcgta gcgcagcatc
     2881 agtttggtgg cctgtgaaaa gcgcgccggg ttggtgaaac tgctcagggt cgccatgttg
     2941 gtgatatcgc cttcctgctc ccagccataa aggtagacat cgagctgctc gttgcgaccc
     3001 ttgacccgca agcggtcgta gggacgcatg ttactcgaca gttccgggtt cctgaccatt
     3061 tcggcgattt cctgggtgca caggatctgg cgtgccttgg ccatgctggc gatgcgcgcc
     3121 gccacgttga ccgtatcgcc gaaaatatcg tctttttcga gtatgatgct gccgtactgc
     3181 atgccaatgc gaatcgatac gccgaccgtt tccggggtgc gatgatcttc catggtttcc
     3241 tggatttcgc gcgcggcgtt gaccgctgcg tcagcatcga tgaagtaaac caggatttcg
     3301 tcgccgatgg ttttgaccag cttgccgtcg tgactgccgg aaatcgaaat cagcgcgtcg
     3361 agagccttcg aaatacgctc cctggcgagt tcgtcaccca tgttttcgta catcgaggtg
     3421 ctgcctgcga catcggcaaa catcaccacg caattaatcg tatctcgcgc caccaaccga
     3481 attccttcgt tatttgcagc ctgggccgtc cgctacggga ccgctaccgg gagccagccc
     3541 ggagccagca cgaccgaccg cggccagtcg tcgccctttg catcttttta tcgcggcgaa
     3601 gtctaacatg gcccgtctga atcttcctta agtttatata gcaggttact gaatataata
     3661 aagaatcaat ccgcgcgggc ggtcgagaca aattctatcg cccggtctac ccgtgccaga
     3721 ctggtctgtc tgcccagcaa ttcgagggtc tggtcgatgg gcggtgaaaa cgagccgccg
     3781 gtaaccgcga cgcgcagcgg ctggccgatc ttgcccatgc cgacgccgag ctccccggtc
     3841 aattgctcga tagccgcatg gatcgcggcc gcgttccagg attcgagtgc ggccaggcgc
     3901 tcgcgg
//
LOCUS       DATCZV010000178         8528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_489619, whole genome shotgun sequence.
ACCESSION   DATCZV010000178 DATCZV010000000
VERSION     DATCZV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489619"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VIW27_03960"
     CDS             <1..1568
                     /locus_tag="VIW27_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5738852.1"
                     /translation="EQSDERLAAESKRVGYPQLIKASAGGGGKGMRLVEQAGQFQAAL
                     ESARREAASAFGDDRMLIERYLVKPRHVEMQVFADSHGNCVHLFERDCSIQRRHQKIL
                     EEAPAPGVDEDLRARMGQAAVDCAQAIDYVGAGTVEFLLDADGEFFFMEMNTRLQVEH
                     PVTEMITGLDLVEWQLRVAAGEALPLAQQQITCSGHAFEARVYAENPQRDFMPAAGRI
                     RYLAEPAREDGLRIDTGVRNGDQIGIFYDPMIAKLIVHGSDRSTALQRLAHALHGYRI
                     LGLQTNVGFLARLAAIPELDAGEVDTGFIERHRASLFANQDEQLRLAAILAAAALLPG
                     LDIDAADAGSPWESRSHWRLNLRTVENICLEAGGHRFEIEAEREAGHWRLRLDDRDYR
                     VAGVRLDATHMRVRVDGRRFEYPVMRAADGIDLCLQGSAYEFDIPDAGRAADAMAVDA
                     SQPRAPMSGAVVALPVAVGDTVEPGATLIVIEAMKMEHAIVAEAGARVAEIHFAVGDQ
                     VDEGDTLLLLEVD"
     gene            1573..2481
                     /locus_tag="VIW27_03965"
     CDS             1573..2481
                     /locus_tag="VIW27_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY5738853.1"
                     /translation="MSFPAQVKIVEVGPRDGLQNESVTVPAEVKIRLIEMLADAGLPV
                     IESGAFVSPKWVPEMATTAEVMRRVERRAGVSYPVLVPNLKGLELALECGVEEIALFA
                     AASETFSQKNTNCSIAESIERFDAVIDAARQAELRIRGYISCVLGCPYEGEVDFDTVA
                     MIADKLFERGCYEVSLGDTIGVGTAGRARELVELLARKLPVERLAAHFHDTYGQALAN
                     IHAVMQCGVAVIDSSVAGLGGCPYAKGATGNVATEDVIYMLDGMGIETGVDMQRLLEA
                     GLYISDFLGREPVSRAATALLRKRAG"
     gene            2484..3062
                     /locus_tag="VIW27_03970"
     CDS             2484..3062
                     /locus_tag="VIW27_03970"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEY5738854.1"
                     /translation="MELPEPASREKKHRRSIECEGFLREDGLWDIEARVVDTRTYDCT
                     YDEFHRDGLIRAGEPVHDMWLRLTIDLDFEIHDVQAASVHTPFSICPRATEAMRDLVG
                     LRIGSGWIRQARERIGTSRSCTHLMDLLGPLAATAYQTLHAALEEREAAQPTRTRPPI
                     LDTCLALSSSGEVVRNLWPEFYRPEPGQESES"
     gene            3059..3727
                     /locus_tag="VIW27_03975"
     CDS             3059..3727
                     /locus_tag="VIW27_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019594365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY5738855.1"
                     /translation="MTVALAFDIYGTLIDPHGVVTQLRQTLGERASEFSRVWRDKQLE
                     YTWRRGLMRRHADFGVCTRQALDYADLLLETGLDEASKQALMGAYRELPAYDDVPAAL
                     ERLRGADCRLYPFSNGTAEMVAAVLEHAGIDGYFEDIISVDSLQTFKPDPEVYRHVIA
                     VAGVDPAACWLVSSNGFDVIGAVAAGMKAAWLQRFSGVVFDPWEIEPTARIASLDELP
                     GVVG"
     gene            3975..4361
                     /locus_tag="VIW27_03980"
     CDS             3975..4361
                     /locus_tag="VIW27_03980"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY5738856.1"
                     /translation="MYEDFVKLTQDSLKPVMKMAENNTAMAVKLMQRQTENASEMMQG
                     NLNHMKALMETKDLNAAVEMQQKYVEGLNEKLIDSAKENAAVIEAAIAEAGKIFEGSF
                     AEVQAQAKKTVEKMEKEISKAGKKAA"
     gene            4481..5659
                     /locus_tag="VIW27_03985"
     CDS             4481..5659
                     /locus_tag="VIW27_03985"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5738857.1"
                     /translation="MANVVIVDGVRTPIGAFGGTLAGTPASKLGAVVIKALLERNGIA
                     PEQVDEVILGQILTAGTGQNPARQAAMEAGLPQECPSMSINKLCGSGLKAVHLAAQAI
                     KCGDADVVIAGGQENMSIAPHLLPRSREGQRMGDWKMEDSMLKDGLMCAFNDYHMGVT
                     AENIAKKWGLSRDDQDAFAAASQQKCEQAQADNVFANEIAAVTIPQRKGDPVVFDRDE
                     YPKAGVTAEGLSKLRPAFDREGTVTAGNASGINDGAAAVLVMSEDKAKELGLTPMATI
                     VSYASAGVDPAIMGTGPIPSTRKALDKAGWKVDDLDLIESNEAFAAQSMSVNAELGWD
                     TDKVNISGGAIALGHPVGASGARVLVTLLHGMQRTDAKKGLATLCIGGGQGVALTVER
                     "
     gene            5695..6417
                     /gene="phbB"
                     /locus_tag="VIW27_03990"
     CDS             5695..6417
                     /gene="phbB"
                     /locus_tag="VIW27_03990"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY5738858.1"
                     /translation="MSRVALVTGGTRGIGEAISLALKDAGYTVVANYAGNDEKASSFS
                     EQHGIPVYKFDVGDYEACQTTVAQIESDVGPVEVLINNAGITRDGTMHKMTFEQWNQV
                     IQTNLTSCFNMCHAVLNGMRERGFGRIVNIGSVNGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGAAKGITVNAIAPGYIATEMVRAVPENVLEKIVQRIPVGRLGEAGEIARGVAFL
                     VADDAGFITGSTLSINGGQHMY"
     gene            complement(6832..7239)
                     /locus_tag="VIW27_03995"
     CDS             complement(6832..7239)
                     /locus_tag="VIW27_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738859.1"
                     /translation="MFKRFIGWFAAVAAVIGVLLASQYFFQPMSSDLSAIGQGQPALV
                     LAYENFSPESGAVLNQLNRIRKDYQGVMNFVVADLGTPQGQAFARQHNLSNGAAVFLS
                     AEGKPLSAAFLTSDEQALRKMLDENLLLAGIAR"
     gene            complement(7731..>8528)
                     /locus_tag="VIW27_04000"
     CDS             complement(7731..>8528)
                     /locus_tag="VIW27_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5738860.1"
                     /translation="VLLFYLQQRTLRQAFEVFDWLRANLTAIRQKQPETARVSMPSEV
                     APLVAEIEKLVEHLRHRIVRTRNAMGNLAHELKRPLQLLSIQQQSAAGTDMQDTLGEI
                     RNILERELRRARISGSTGAIGTFDIAVETESMTEVLRKIYPDINLEVELRSRVVTSDL
                     DRDDMLELLGNLLDNACKFARTRVRLTVDAIDNHLELVFEDDGQGLAADQLQLLQRRG
                     RRLDERVAGHGLGLGICRDILDYYHGELAFTESELGGLSVTARIPFT"
BASE COUNT         1683 a         2623 c         2691 g         1531 t
ORIGIN      
        1 acgagcagag cgacgagcgc ctggccgccg aatcgaagcg cgtcggctac ccgcaattga
       61 tcaaggccag cgccggcggt ggcggcaagg gcatgcggct ggtggagcag gccggccagt
      121 tccaggccgc gctcgaatcg gcgcgccgcg aggccgcgtc ggcgttcggc gacgatcgca
      181 tgctgatcga acgttacctg gttaaaccgc ggcacgtcga gatgcaagtg tttgccgata
      241 gccacggcaa ctgcgtgcac ctgttcgaac gcgactgctc gatccagcgg cgtcaccaga
      301 aaatcctcga ggaagcgccg gcgcccggcg tcgacgaaga tttgcgcgcg cgcatggggc
      361 aggccgcggt ggattgcgcg caagccatcg actatgtcgg cgccggtacc gtcgaattcc
      421 tgctcgacgc cgacggcgag ttcttcttca tggaaatgaa tacccgtcta caggtcgagc
      481 acccggtcac cgagatgatc accgggctcg atctggtcga gtggcagttg cgggtcgccg
      541 ccggcgaagc cctgccgctg gcgcagcagc agatcacctg cagcggccac gccttcgagg
      601 cgcgcgtcta cgccgaaaat ccgcagcgcg acttcatgcc ggccgccggg cgcattcgct
      661 acctggcgga gccggcgcgc gaggacgggt tgcgcatcga taccggggta cgcaacggcg
      721 accagatcgg cattttctac gacccgatga tcgccaagct gatcgtgcac ggcagtgatc
      781 gttcgaccgc gctgcaacgg ctggcgcacg cgctgcacgg ctaccgaatc ctcggcctgc
      841 aaaccaacgt cggtttcctc gcgcggctcg cggcgatacc ggagctcgac gccggcgaag
      901 tcgataccgg cttcatcgag cgccaccgcg caagcctgtt cgcgaaccag gacgaacagt
      961 tgcggctggc cgcgatactc gccgccgcgg cgctgctgcc cggcctcgac atcgacgccg
     1021 cggatgcggg ctcgccgtgg gaatcacgct cgcactggcg actcaacctg cgcacggtcg
     1081 agaatatttg cctcgaggcc ggcggccacc gcttcgagat cgaggccgaa cgcgaggccg
     1141 gtcactggcg cctgcggctc gacgaccggg attaccgcgt cgccggtgtc aggctcgacg
     1201 cgacgcacat gcgcgtgcgt gtcgacggcc gccgcttcga gtacccggta atgcgcgccg
     1261 ccgacggcat cgacctgtgc ctgcagggca gcgcttacga attcgatatt ccggacgccg
     1321 gccgcgccgc ggacgcgatg gcggtcgacg ccagccaacc gcgcgcgccg atgtcggggg
     1381 ccgttgttgc gctgccggtg gcggtcgggg atactgtcga gcccggcgcg acgctgatcg
     1441 tcatcgaggc gatgaagatg gaacacgcca tcgtcgccga ggccggcgcg cgggtcgcgg
     1501 agattcactt cgcggtcggc gaccaggtcg acgaaggcga tacgctgctg ttactggagg
     1561 tcgattgagt cgatgagttt ccccgcccaa gtcaagatcg tcgaggtagg gccgcgcgac
     1621 ggcctgcaaa acgaatcggt cacggtaccg gccgaggtca agattcggtt gatcgaaatg
     1681 cttgccgacg ccggcctgcc ggtgatcgag tccggagcct tcgtctcgcc gaagtgggtg
     1741 ccggaaatgg cgaccacggc tgaagtgatg cggcgcgtcg agcggcgcgc cggcgtctcc
     1801 tatccggtgc tggtgcccaa tctgaagggc ctcgaactgg cgctcgaatg cggcgtcgag
     1861 gaaattgcgc tgttcgccgc cgccagcgag accttctcgc aaaagaacac caattgctcg
     1921 atcgccgaga gcatcgagcg cttcgacgcg gtgatcgatg ccgcgcggca ggcggagctg
     1981 cggattcgcg gctatatctc ctgcgtgctc ggctgcccgt atgagggcga ggtcgatttc
     2041 gataccgtgg cgatgatcgc cgataagctg ttcgaacgcg gctgctacga ggtttcgctc
     2101 ggcgacacca tcggcgtcgg taccgcgggg cgtgcccgcg aactggtcga actgctggcc
     2161 cgcaagctgc cggtcgagcg gctggcggcg cattttcacg atacctacgg tcaggcgctg
     2221 gcgaatattc acgcggtcat gcaatgcggc gtcgcggtga tcgacagttc ggtcgccggt
     2281 ctcggcggtt gtccctatgc aaagggcgcg accggcaacg tcgccaccga ggacgtgatc
     2341 tacatgctcg acggcatggg gatcgaaacc ggcgtcgaca tgcaacgctt gctggaggcg
     2401 ggtctttaca tcagcgattt tctcggccgc gaaccggtat cgcgcgcggc gaccgcgctg
     2461 ctgcgcaaga gggcgggttg agcatggaat tgcctgaacc cgccagccgc gaaaagaaac
     2521 accggcgcag tatcgaatgc gagggcttcc tgcgcgagga cggcctctgg gacatcgagg
     2581 cgcgcgtcgt cgatacccgc acctacgatt gcacctacga cgagtttcac cgtgacggcc
     2641 tgattcgcgc cggcgagccg gtgcacgata tgtggctgcg gctgaccatc gatctcgatt
     2701 tcgagataca cgacgtgcag gccgcctcgg tgcacacgcc gttcagtatc tgtccgcgcg
     2761 ctactgaggc gatgcgcgac ctggtcggcc tgcgtatcgg ctccggctgg atcaggcagg
     2821 cgcgcgaacg catcggcacc agccgcagtt gcacgcacct gatggatttg ctcggcccgc
     2881 tcgcggccac cgcctaccag acgctgcacg ccgccctcga ggagcgcgaa gccgcgcagc
     2941 cgacgcgtac caggccgccg atcctggaca cctgcctggc gctgtcgagc agcggcgagg
     3001 tggtcaggaa cctgtggccc gaattctatc ggcccgaacc cgggcaggag tccgaatcat
     3061 gaccgttgca ctggctttcg atatctacgg cacgctgatc gatccgcacg gcgtggtcac
     3121 gcagttgcgg caaacgctcg gcgagcgggc cagcgagttt tcgcgggtgt ggcgcgacaa
     3181 gcagctcgag tacacctggc ggcgcggact gatgcggcgt catgccgatt tcggtgtctg
     3241 cacgcgccag gcgctcgact atgccgacct gttgctggaa accggtctcg acgaggcgag
     3301 caaacaggcg ctgatgggcg cctaccgcga attgccggcc tacgatgatg taccggcggc
     3361 tctcgagcgg ttgcggggcg cggactgccg tctctacccg ttctccaacg gcaccgccga
     3421 aatggtcgcc gcggtgctcg agcacgccgg catcgacggc tacttcgagg acatcatcag
     3481 cgtcgacagc ctgcaaacct tcaagcccga ccccgaggtt taccggcacg tgatcgctgt
     3541 cgccggcgtc gacccggccg cttgctggct ggtgtcgagc aacggcttcg atgtcatcgg
     3601 cgcggtcgcc gcgggcatga aggcggcctg gctgcagcgc ttcagcggcg tggtattcga
     3661 tccctgggag atcgagccga cggcgcgcat cgcgagtctc gacgagttgc ccggggtggt
     3721 cggctgactc gcgcccgaat cgctgccaac accaccccgt gaggacgaaa atttttcgca
     3781 aaaattgccc gcaatgcttt cccgcacttt tatagttcaa ggtaaatctt tatatttcaa
     3841 taacttgaat tattgaaatt atttttgttg cgctgcaaca tttttcttga catcggcgtg
     3901 catgacccct atattatgtc cattgctgca gtgcagcata agtttactaa cccgatctta
     3961 tagaggtaat taacatgtac gaagatttcg taaagctgac ccaggacagc ctcaagccgg
     4021 tcatgaaaat ggctgaaaac aacaccgcta tggcggtcaa gctgatgcag cgccagaccg
     4081 agaacgcttc ggaaatgatg cagggcaacc tgaaccacat gaaagcgctg atggaaacca
     4141 aggacctgaa cgctgctgtt gaaatgcaac agaagtacgt cgaagggctc aacgagaagc
     4201 tgatcgactc ggccaaggaa aatgccgcgg taatcgaagc agccatcgcc gaagccggca
     4261 agattttcga aggctctttc gccgaagttc aagcccaggc caagaaaacc gtcgagaaga
     4321 tggaaaagga aatcagcaag gctggcaaga aagctgcctg aaccgcttag cggagattgc
     4381 tccggcccag gccggagcga tcgctccaaa gtttactcct ccaaccttgg gcccggtggt
     4441 attattaccc ccgggctttt ttttaagcag gtgacagaat atggcaaacg tagtgatagt
     4501 tgacggggtg cgcaccccaa ttggcgcgtt cggcggcacg ctggcgggaa cgccggctag
     4561 caagctgggc gcggtcgtga tcaaggccct gctggagcgc aacggcattg ctccggaaca
     4621 ggtcgacgaa gtgatcttgg gacagatttt gactgccggc accgggcaga acccggcccg
     4681 ccaggcggca atggaggccg gcctgccgca ggaatgcccg tcgatgtcga tcaacaagct
     4741 gtgcggcagc ggcctcaagg ccgtgcatct tgccgcgcag gcgatcaagt gcggcgacgc
     4801 cgatgtcgtc atcgccggcg gccaggaaaa catgagcatc gcaccgcacc tgctgccgcg
     4861 gtcgcgcgaa ggccagcgca tgggcgactg gaagatggaa gactcgatgc tgaaggacgg
     4921 cctgatgtgc gccttcaacg actaccacat gggcgtgacc gccgagaata tcgccaagaa
     4981 gtggggcctg agccgtgacg accaggatgc cttcgccgcc gcttcgcaac agaaatgcga
     5041 gcaggcgcag gccgacaacg tcttcgccaa cgaaatcgcg gcggtcacga ttccgcagcg
     5101 caagggcgac ccggtcgtct tcgaccgcga cgaatacccg aaggccggtg tcaccgccga
     5161 gggcctgtcc aagctgcgtc ccgccttcga ccgcgaaggt accgttaccg cgggcaacgc
     5221 ttccggcatc aacgatggcg ccgccgcggt gctggtaatg agcgaggaca aggcgaaaga
     5281 actcggcctg acgccgatgg cgaccatcgt ctcctacgcg agtgctggtg tcgaccccgc
     5341 gatcatgggt accggcccga tcccgtcgac ccgcaaggcg ctggataaag cgggctggaa
     5401 agtcgatgat ctcgacctga tcgaatccaa cgaggcgttt gccgcgcaat ccatgtcggt
     5461 caacgccgaa ctcggctggg acaccgacaa ggtcaacatc agcggcggcg cgatcgcact
     5521 cggccacccg gtcggcgctt ccggcgcgcg tgtactggtc accctgttgc atggcatgca
     5581 gcgcaccgac gcgaaaaagg gcctcgccac gctgtgcatc ggcggcggtc agggcgttgc
     5641 cctgacggtg gagcggtgaa taatacgaaa cacaacctat aaaggagaga cactatgagc
     5701 agagtagcgc tggttaccgg cggcacccgc ggcatcggcg aagccattag cctggccctc
     5761 aaggatgccg gctacacggt ggtcgccaac tatgccggta acgacgaaaa agccagcagt
     5821 ttcagtgaac aacacggcat tccggtctac aagttcgacg tcggcgatta cgaagcctgc
     5881 cagacgacgg tggcgcagat cgagagtgac gtcggtccgg tcgaagtgct gatcaacaac
     5941 gccggcatca cccgtgacgg caccatgcac aagatgacgt tcgagcaatg gaaccaggtc
     6001 attcaaacca acctgacctc gtgcttcaac atgtgccacg cggtattgaa cggtatgcgc
     6061 gagcgcggtt tcggccgcat cgtcaacatc ggttcggtca acgggcaggc cggccagtac
     6121 ggccaggtca actatgctgc cgcgaaatcg ggtatccacg gtttcaccaa ggcgctggcg
     6181 caggaaggcg ccgccaaggg aatcaccgtc aatgccatcg cgccgggcta catcgccacc
     6241 gagatggtgc gcgcggtgcc ggagaacgtg ctcgagaaga tcgtgcagcg gattccggtc
     6301 ggccgtctcg gcgaggccgg cgaaatcgcc cgcggtgtgg ccttcctggt cgccgacgac
     6361 gccggtttca tcaccggctc gacgctgtcg atcaacggtg gccagcacat gtactaggcc
     6421 gccggcgagt cgcgcaacaa ccgcgggcgg agtccgcggg tttcgcgaca ggtcgagggc
     6481 tggcggtttg cggactgccg atgctcgaga cacgccaacc ggcatcatgg ccgctgctca
     6541 cgggcagcgg cttttttaag cccggtgcca cgggccgagc gtgtatcgcg cgtcgagcgg
     6601 ggatgaattc cgcgttcgag ggccagcgcc cgccagcctg gaaagccggc attcgaccag
     6661 aggtagccag actggactgg ctaccgaatc gactgtaatt tcaccgcgaa aatcggccgg
     6721 gcaagctatc gggcctgcct gatggattcc ggccggtgcg cgcaggcttt cgccaggatg
     6781 acaaattaaa gggtaccgtc ccctttgccc cggatacctg ctaccggatt tctaccgagc
     6841 aattcctgcc aggagaagat tttcatccag cattttacgc aaggcctgct cgtcgctggt
     6901 caaaaaagcg gcgcttagcg gtttaccttc ggctgataaa aacaccgccg caccattact
     6961 gagattgtgt tgtctggcga atgcctggcc ttgtggcgtg cccagatcag ccaccacaaa
     7021 gttcatgacg ccctgataat ctttcctgat gcgattcagc tggttgagca ccgcaccgct
     7081 ttcaggtgag aagttttcgt aagccagtac cagtgcaggc tggccttgcc caatggctga
     7141 gagatcagaa ctcattggct gaaagaaata ttggctcgcc agcaaaacac cgatgactgc
     7201 cgccactgcg gcaaaccaac ctatgaatcg tttaaacatg aatacctgtc atacctcgct
     7261 gactattgtg agtgacttta aaaatggcca tcatttgcca ggctcgatca aaatccgggc
     7321 catggatgaa gctacaatct cccgggtgac cgccgaatta gaaattatcg accggcttgc
     7381 tagaccctgg cgatctcaat aatgccatct tccacgttat caaagaaagg ggtgtatcag
     7441 agataaggga ttagttacaa acgagtatta ttagcacact gagcccgaga ccatctgtca
     7501 ccaaccactt tccctggccc tttcctccct gtgcccgcta ctgtatggct gttcgagccg
     7561 gcactgtcag aaatgatgcg gggacttgcg cggttatgaa atgttcgggc attgcccggc
     7621 gaatcatcgg tcatcgctat cgcgacagtg tttcccgggc accgggcagc ttcggaagag
     7681 tgccgctgcg gatacctatc ccggtgccac gccggcgacg gggctcgcgg ctaggtaaac
     7741 ggaattctgg cggttaccga tagcccgccc agttcggact cggtgaatgc cagctcgccg
     7801 tggtagtagt ccaggatatc gcggcaaatg cccagcccca atccatgccc ggcgacacgc
     7861 tcgtccagcc gccggccccg ccgctgcagc aactgcagct gatcggcggc gagcccctgg
     7921 ccgtcgtctt cgaagaccag ttcgaggtgg ttatcgatcg cgtcgaccgt cagacgcacc
     7981 cgggtacgcg cgaacttgca ggcgttgtcg agcagattgc cgagcagttc gagcatgtca
     8041 tcgcgatcca ggtccgaggt tacgacccgg ctcctgagtt cgacctcgag gttgatatcg
     8101 gggtatattt ttcgcagcac ctcggtcatc gactccgttt ccacggcgat gtcaaaggta
     8161 ccgatggcac cggtcgagcc ggagattctt gctcgcctca gttctcgctc caggatattc
     8221 ctgatctcgc cgagcgtgtc ctgcatatcc gtgcccgctg cgctctgctg ctggatcgac
     8281 agtaattgca gcgggcgctt gagttcgtgc gcgaggttgc ccatcgcgtt tcgggtgcgc
     8341 acgattcgat ggcgcaggtg ttcgaccagc ttttcgattt cagcaaccag cggggcgacc
     8401 tcggacggca tcgacacccg cgcggtttcg ggttgctttt gccggatcgc cgtcaggttg
     8461 gcacgcaacc agtcgaacac ctcgaaagcc tggcgcaaag tgcgttgctg caggtaaaaa
     8521 agcagtac
//
LOCUS       DATCZV010000179         4132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_65967, whole genome shotgun sequence.
ACCESSION   DATCZV010000179 DATCZV010000000
VERSION     DATCZV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65967"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="VIW27_04005"
     CDS             complement(<1..2177)
                     /locus_tag="VIW27_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY5738861.1"
                     /translation="MKETALSARSATSLQDQAPADVGVDSAALERRLRAELEGEVLFD
                     AFSRGRYSTDASIYQIEPLGVVVPRNEQDVVRALQVAADHGVPLLPRGGGTSQIGQTV
                     GRALVVDTSKYLCRVRDFDPESRRVVVQPGTVLDQLNRQLKPHGLFFPVDVSTGNRAT
                     LGGMAGNNSCGARSIRYGNMVHNVRAIDAILADGNRAHFAGMQQPEGNGSFARLVERM
                     AALGAREAGEIEARFPRLLRRVGGYNIDQLASPEDHSMARLLVGSEGTLAFFTGLELE
                     LQPIPPHKVLGICQFPSFYQAMDSTRHIVELGPSAVELVDRTMIELSREIPLFRDTVE
                     RFVNGEPDALLLVEFAGENAQQQLERLARLVELMGDLGFPGAVVEALDPDFQSAVWEV
                     RKAGLNIMMSMKGDGKPVSFIEDCAVQLEDLAEYTDRLNRVFEKHGTTGTWYAHASVG
                     CLHVRPILNMKDASGARKMRAIMEETLEIVQQYKGSHSGEHGDGIARSEFHERMFGAR
                     MQRAFEEVKDSFDPAGLFNPGKIVRAPRMDDRSLFRYAPGYRVEPIDTALDWSEWGGF
                     GGAVEMCNNNGACRKANVGVMCPSYRATRDEQHSTRGRANTLRLAISGQLGADALLSD
                     SMLRTMELCIGCKGCKRECPTGIDMAKMKVEFLYHYRKRHGLPLGERLIAYLPRYAPV
                     ASRLGWLLNLRDRLPGLAWLSQQLFGFSARRSLPRWRRDYFDDR"
     gene            2323..3492
                     /locus_tag="VIW27_04010"
     CDS             2323..3492
                     /locus_tag="VIW27_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5738862.1"
                     /translation="MSYRPGRHFLQIPGPTNVPDRVLRAMDMPTSDHRGPQFAGLTRE
                     ILDGLKAVFKTSAEVVVYPSSGTGAWEAALVNTLSPGDRVLMFETGQFATLWRGIATD
                     LGLEVEFVPGDWRHGVDPAEVETRLAADSEQAIKAVAVVHNETSTGVTSRIGRIREAI
                     DRAGHSALLLVDVISSLGSIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNAISAKALAA
                     AENSTLPAAYWRWQPMLDNNARGYFPYTPATNLLYGLREALRMLHEEGLENVFARHRR
                     HGEATRRAVRAWGLEVLCLEAEEYSDALTAVLLPEGEDADRLRQVILERFDMSLGTGL
                     GKLQGRVFRIGHLGDFNDLMLAGTLCGVEMGLAAAGIAFNRGGVNAALEYLAAAS"
     gene            3546..>4132
                     /locus_tag="VIW27_04015"
     CDS             3546..>4132
                     /locus_tag="VIW27_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020058824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY5738863.1"
                     /translation="MSKAESDGFLQLWPTTLLQRTLPGHEAANRALAALIEQLESANR
                     ELTTDYLRDNLLTHEHPAIEWLRECINRTAVDYLQRQGLDYPVDWTLHGWANINRLGD
                     YHDLHNHPHSYLSGTYYVAMPSTAARAAGSRNDLNPGAISFYDPRPQANMNAIRGDAQ
                     ISPQHTIQPRPGMILMWPAFLHHLVHPNLSDEKRIS"
BASE COUNT          761 a         1373 c         1313 g          685 t
ORIGIN      
        1 cggtcgtcga agtaatcgcg acgccagcgc ggcaggctgc gccgcgcgga aaatccgaac
       61 agctgctggc tcaaccaggc cagccccggc aagcggtcgc gcaggttcaa caaccagccg
      121 agacgtgacg ccaccggcgc atagcgcggc aggtaggcga tcagtcgctc gccgagcggc
      181 agtccgtgac gcttgcggta atggtaaaga aattccacct tcatcttcgc catgtcgata
      241 ccggtcgggc attcgcgctt gcagcccttg caaccgatgc acagctccat ggtgcgcaac
      301 atcgaatcgg acagcagcgc atcggcgccg agctggccgc tgatcgccag gcgcagggtg
      361 ttggcgcgcc cgcgcgtcga gtgctgttcg tcgcgggtcg cgcggtagga cgggcacatc
      421 acgccaacat tggccttgcg gcaggcaccg ttgttgttgc acatctcgac cgcgccgccg
      481 aaaccgcccc actccgacca gtcgagcgcg gtgtcgatgg gttcgacccg gtagcccggc
      541 gcatagcgaa acaggctgcg atcgtccatc cgcggcgcac gcacgatctt gcccgggttg
      601 aacaagccgg cgggatcgaa actgtccttg acctcctcga aagcgcgctg catgcgcgca
      661 ccgaacatgc gctcgtggaa ctcggagcgc gcgataccat cgccatgctc gccggaatgt
      721 gagcccttgt actgctgcac gatttccagc gtctcttcca tgatcgcgcg catcttgcgc
      781 gcgccgctgg cgtctttcat gttcagaatc ggccgcacgt gcaggcagcc gaccgaggca
      841 tgcgcatacc aggtgccggt ggtgccgtgc ttttcgaata cccggttcag gcggtcggtg
      901 tactcggcca ggtcctcgag ctgcaccgca cagtcctcga tgaaggatac cggcttgccg
      961 tcgcccttca tcgacatcat gatgttgagc ccggccttgc gcacttccca gacagccgac
     1021 tggaagtcgg gatctagcgc ctcgacgacg gcgccgggaa atcccaggtc gcccatcaac
     1081 tcgaccagcc gcgcgagccg ctcgagttgc tgctgcgcat tctcgccggc gaactcgacc
     1141 agcaacagcg cgtccggctc accgttgacg aaacgctcga cggtatcgcg aaacagcgga
     1201 atctcgcgcg acagctcgat catcgtacga tcaaccagct cgaccgcgct cggccccagc
     1261 tcgacgatgt ggcgggtcga atccatcgcc tggtaaaagc tcgggaactg gcaaatgccg
     1321 agcaccttgt gcggcggaat cggctgcaac tcgagctcga ggccggtgaa aaaggccagc
     1381 gtgccttccg agcccaccag caaccgcgcc atcgagtggt cttcgggact ggccagctgg
     1441 tcgatgttgt agccaccgac ccgccgcagc aagcgcggaa agcgcgcctc gatttccccg
     1501 gcctcgcgtg cgccgagggc cgccatccgt tccaccaggc gggcgaagct gccgttgcct
     1561 tcgggctgct gcatgccggc gaagtgcgcg cgattgccgt cggcgagtat cgcgtcgatg
     1621 gcgcgcacgt tgtgcaccat gttgccgtag cgaatcgagc gcgcgccaca gctgttgttg
     1681 ccggccatgc cgccgagcgt ggcgcggtta ccggtcgaca cgtcgaccgg aaagaacagg
     1741 ccatggggtt tgagctggcg attgagctgg tcgagcacgg taccgggctg gaccacgacg
     1801 cggcgcgatt cgggatcgaa atcgcgcacc cggcacaggt acttgctggt gtcgacgacc
     1861 agcgcgcggc cgaccgtctg gccgatctgc gaggtgccgc caccgcgcgg cagcagcggg
     1921 acgccatgat cggcggctac ctggagggcg cgcacgacat cctgctcgtt gcgcggcacc
     1981 accacgccga gcggttcgat ctgatagatc gacgcatcgg tactgtagcg gccgcgactg
     2041 aatgcgtcga acagcacctc accctcgagt tcggcgcgca gccggcgttc aagggcggca
     2101 ctgtcgaccc cgacgtcggc gggagcctgg tcttgcagcg aagtcgcgga cctcgccgac
     2161 aaagcggttt ctttcatcga ttaaagcggt ttaaactgcg cagacgccgg tgattcggcg
     2221 ccggttttag gtgcaagcaa cgataacaat gatcgaaaac catctcaact gcaaccaccc
     2281 gatgcgcaag ccacgcaccg ggacatttcg gggaaaatca tcatgagcta tcgaccgggg
     2341 cgccattttc tgcagatacc cggccccacc aacgtgccgg accgggtact cagggcgatg
     2401 gacatgccaa ccagcgacca tcgcggtcca cagttcgccg ggctgacgcg tgaaattctc
     2461 gatggcttga aagcggtgtt caagacctcc gcggaggtgg tcgtctaccc gtcctcgggt
     2521 accggtgcct gggaagcggc gctggtcaac accctgtccc ccggcgaccg ggtgctgatg
     2581 ttcgagaccg gccagttcgc gaccctgtgg cgcggcatcg ccaccgatct cggtctcgag
     2641 gtcgagttcg tgcccggcga ctggcgtcac ggcgtcgacc cggccgaggt cgagacgcgg
     2701 ctggcggcgg attcagagca ggccatcaag gcagtggccg tagtgcacaa cgagacctcc
     2761 accggcgtca ccagccgcat cggccgcatt cgcgaagcga tcgaccgcgc gggccattcg
     2821 gcactgctgc tggtcgacgt catttcgtcg ctgggatcga tcgattaccg gcacgacgaa
     2881 tggggcgtcg acgtcaccgt ggccggctcg cagaaaggct tgatgctgcc accggggctg
     2941 agcttcaacg cgatcagcgc gaaagcgctg gcggccgccg agaactccac gttgccggcc
     3001 gcctactggc gctggcaacc aatgctcgac aacaacgcgc gcggctattt cccgtatacc
     3061 ccggcgacca acctgctgta cggcttgcgc gaggcgttgc gcatgctgca cgaggaagga
     3121 ctcgaaaacg tgtttgcacg ccaccggcgt cacggcgagg cgactcgccg cgcggtgcgc
     3181 gcctgggggc tcgaggtgct gtgtctcgag gccgaggaat acagcgatgc gctgaccgcg
     3241 gtattgctgc ccgagggcga ggatgccgac cgcctgcgac aagtcatcct cgagcgcttc
     3301 gacatgtcac tcggcaccgg cctcggcaaa ttgcaggggc gcgtgtttcg catcggtcat
     3361 ctcggcgatt tcaatgacct gatgctggcg ggtaccctgt gcggggtcga aatgggcctc
     3421 gccgcggccg gcatagcgtt caaccgcggc ggcgtcaatg cggcactcga gtatcttgcc
     3481 gcggcatcct gagccgcgca cagcgttcga tcgccggttc gaaacaggta tggtgagaca
     3541 agcggatgtc gaaagcggaa agtgacggat tcctgcaact gtggccgacc acgttgctgc
     3601 agcgcacgct acccggtcac gaggccgcca accgggccct cgcggcactg atcgaacagc
     3661 tcgaatccgc gaaccgcgag ctgaccaccg attacctgcg cgataacctg ctgacccacg
     3721 agcatccggc gatcgagtgg ctgcgggagt gtatcaaccg aaccgcggtg gattacctgc
     3781 agcgccaggg cctcgattac ccggtcgact ggaccctgca cggctgggcc aacatcaacc
     3841 ggctcggcga ttaccacgac ctgcacaacc atccgcacag ttacctgagc ggcacttact
     3901 acgtggccat gccgtcaacc gcggcgcggg cggcgggcag ccgcaacgac ctcaaccccg
     3961 gcgcgatctc gttttacgat ccgcgcccgc aggccaacat gaatgcgatt cgcggcgacg
     4021 cgcagatatc gccgcagcat acgattcagc cgcggccggg aatgatactg atgtggcccg
     4081 cctttctgca ccacctggtt caccccaacc tgagcgacga aaaacgtatc tc
//
LOCUS       DATCZV010000180         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_278048, whole genome shotgun sequence.
ACCESSION   DATCZV010000180 DATCZV010000000
VERSION     DATCZV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278048"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="gspL"
                     /locus_tag="VIW27_04020"
     CDS             <1..965
                     /gene="gspL"
                     /locus_tag="VIW27_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY5738864.1"
                     /translation="LLGAIEYQIEDRLAQDVESQHFALGDTRQNPISVAVVSREIMDR
                     CMMLAQTHGLRLAQVIPELFLCPWGGQGVSLSEGYDGWLLRYGDYRGLKCNSAALPAM
                     LELVGHEIDFDAIRCYGEAPQLDGVEIEQLALNEARPGFAGAPLIDLQQRDYQLSSPW
                     LGLARTWKWIGLMVLLLLAAFTYNRAVALQNLEHELDEIRQQQYELLQPYLPAASGPN
                     DDLKRMLIERIKQLRASRQEQGFLQLMLEFVRAREKFPDIEISRVGYQGDRLSFDISS
                     SKLNDIEALLASVKQRGVQAKLESLNIKPELSSGRLVMEGGGDA"
     gene            958..1431
                     /gene="gspM"
                     /locus_tag="VIW27_04025"
     CDS             958..1431
                     /gene="gspM"
                     /locus_tag="VIW27_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY5738865.1"
                     /translation="MLEYFHRFTPRERAIVMLALLVAIGVGLHALVIEPFQLRFAELE
                     EAIEQQRADYEWMRTAVAEMPNVEIADDAVQIDGTLANFINQAVNRQGRSAQLSQMSP
                     IGSDEIRMRYNAVDFNRLVNFIAQVNARGLEIKDLRITAADTPGRVDSSLIFVRR"
     gene            1431..2204
                     /locus_tag="VIW27_04030"
     CDS             1431..2204
                     /locus_tag="VIW27_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY5738866.1"
                     /translation="MLGKLWKFTLFFLLCLIVALLFNLPIQQVQPHVTLPPTVRLADI
                     DGTILGGKAREIRINEFPFQNVSYSYQPSCIPLLKVCYRIDYDQGRLQAAYDLLNGDT
                     EINDLRIVYPVSEVVAYVPNLLVNPTGRVELLVDEVSMIAGRPATLAGKLVWRDLGID
                     NDGVRINIGDYELDFVGDTSAYDFELRDRDATLDVDGEGKVSANGDYSFDIRILTDGN
                     LDSNVRYVLNLVAKKTGPSSFRIEQSGRLPPRVRSRIFP"
     gene            complement(2251..>2613)
                     /locus_tag="VIW27_04035"
     CDS             complement(2251..>2613)
                     /locus_tag="VIW27_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEY5738867.1"
                     /translation="AIWGASFKPGSASLDSAPSLMVIDALVAQQAEVRIHDPEALENV
                     QQIYRDMPAVKTIGGKYDALKNVDALLLLTEWPEYWSPDFEIMLRQMKSPVIVDGRNV
                     YNRAQLEALGFTYYGVGR"
BASE COUNT          494 a          809 c          819 g          491 t
ORIGIN      
        1 agttgctcgg cgcgatcgag taccagatcg aggaccgcct ggcacaggac gtcgagtcgc
       61 agcacttcgc gctcggcgat acgcggcaaa acccgatctc ggtcgcggtg gtgtcgcgcg
      121 aaatcatgga tcgctgcatg atgctggcgc agacccacgg attgcgcctt gcgcaggtga
      181 taccggaact gtttctgtgc ccgtggggcg gccagggtgt ctcgctcagc gagggttacg
      241 acggctggtt gctgcgctac ggcgattacc gcgggctcaa gtgcaatagc gcggcgctgc
      301 cggcgatgct cgagctggtc ggccacgaaa tcgatttcga cgcgattcgc tgttacgggg
      361 aggcgccgca actcgacgga gtcgagatcg agcagctggc attgaacgag gcgcgcccgg
      421 ggttcgccgg tgcaccgttg atcgacctgc agcagcgcga ttaccagctg tcgtcgccgt
      481 ggctcgggct cgcgcgaacc tggaaatgga tcggcctgat ggtactgtta ctgctggccg
      541 catttaccta caaccgggcc gtcgcgctgc agaacctcga gcacgaactc gacgagattc
      601 gccagcagca gtacgaattg ctgcaaccct acctgccggc agccagcggg ccgaacgacg
      661 atctgaaacg catgctcatc gagcgcatca agcagttgcg cgccagccgc caggagcagg
      721 gatttctgca gttgatgctc gagttcgtac gcgcccgcga aaagtttccc gacatcgaga
      781 tttcgcgtgt cggttaccag ggcgatcgcc tcagcttcga tatcagcagc agcaagctca
      841 acgatatcga ggcgctgctg gcgagcgtca aacagcgcgg cgtgcaggcg aaactcgaga
      901 gcctcaacat caagcccgag ttgagttctg gccggctggt gatggagggc ggcggcgatg
      961 cttgaatatt tccatcgctt cacgccgcgc gagcgcgcca tcgtcatgct cgcgctgtta
     1021 gttgcgatcg gcgtcggcct gcacgcgctg gtgatcgaac ccttccagct gcggttcgcc
     1081 gaactcgagg aggcgatcga gcaacagcgc gccgattatg agtggatgcg cacggcggtc
     1141 gccgaaatgc ccaacgtcga gatcgccgac gacgccgtgc agatcgacgg cacgctcgcc
     1201 aattttatca accaggcggt caatcggcag ggtcggtcgg cgcagttgtc gcagatgtcg
     1261 ccgatcggca gcgacgaaat ccgcatgcgc tacaatgcgg tggatttcaa ccggctggtg
     1321 aatttcatcg cccaggtcaa cgcccgcggc ctggaaatca aggaccttcg aataaccgcg
     1381 gccgacaccc cgggcagggt cgatagcagc ctgatcttcg tgcgtcgctg atgcttggca
     1441 agctctggaa gttcacgctg tttttcctgc tgtgccttat cgtcgcactg ctgttcaacc
     1501 tgccgatcca gcaggtacag ccccatgtga cgttgccgcc gacggtgcgc ctggcggaca
     1561 tcgacggcac catcctcggc ggcaaggcgc gcgaaatccg tatcaacgag ttcccgtttc
     1621 aaaatgtgag ctacagctac cagccgtctt gcattccgct gctgaaggtt tgctaccgga
     1681 tcgactatga ccagggcagg ctgcaggccg cttacgacct gctcaacggc gataccgaga
     1741 tcaacgacct gcgcatcgtc tacccggtca gcgaggtggt cgcttacgtg cccaatctgc
     1801 tggtcaatcc gaccggccgc gtcgaattgc tggtcgacga agtgtcgatg atagccgggc
     1861 ggccggccac gctcgccggc aagctggtgt ggcgcgatct cggtatcgac aacgacggcg
     1921 tgcgcatcaa catcggcgat tacgaactcg atttcgtcgg tgataccagt gcctacgatt
     1981 tcgaactcag ggatcgcgac gcgacgctcg atgtcgacgg cgagggcaag gtcagcgcca
     2041 acggggatta cagtttcgac attcgtatcc tgaccgacgg caatctcgac tcgaatgtcc
     2101 gctacgtgct caacctggtg gcgaaaaaga ccggccccag cagtttccgc atcgagcaga
     2161 gcggccgcct gccgccgcgc gttagaagcc gcatcttccc ctgaatgccg gccgccgcga
     2221 cggcggaatt cgcgctgacc ggccccggtg ctagcggccg acgccgtagt aggtgaaacc
     2281 cagggcttcc agctgggcgc ggttgtagac gttgcgcccg tcgacgatga ccggcgattt
     2341 catctgccgc agcatgatct cgaaatccgg cgaccagtac tccggccact cggtcagcag
     2401 cagcagcgcg tcgacgttct tcagtgcatc gtacttgccg ccgatggttt tcaccgcggg
     2461 catgtcgcga tagatttgct gcacgttctc gagtgcctcg ggatcgtgaa tccgcacctc
     2521 ggcctgttgc gcgaccagcg catcgataac catcaggctg ggcgccgagt ccagcgaggc
     2581 cgatccgggt ttgaacgaag ctccccagat ggc
//
LOCUS       DATCZV010000181         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_13491, whole genome shotgun sequence.
ACCESSION   DATCZV010000181 DATCZV010000000
VERSION     DATCZV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13491"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VIW27_04040"
     CDS             <1..392
                     /locus_tag="VIW27_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5738868.1"
                     /translation="LQNDTGMALVLITHDMGVVAETAQRVVVQYAGQQVERQPVGGLF
                     DHPHHPYTAALLDALPDRASGDRLPAIPGVVPGQADRPDGCLFNPRCAFADDLCRSTR
                     PAVREGDPTLTLCHYPLAAKRTAGAES"
     gene            389..1357
                     /locus_tag="VIW27_04045"
     CDS             389..1357
                     /locus_tag="VIW27_04045"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY5738869.1"
                     /translation="MTGEPILRAHELTRYYEVKKGTFGESATVKALNGASFELRAGET
                     LAIVGESGCGKSTLARVLTMIEPPTSGRLEIEGREISLAGSRPSKELRQRVQIVFQNP
                     YGSLNPRKTIATMLEEPLKLNTDLDPAQRRRAAEEMIARVGLRPEHADRYPHMFSGGQ
                     RQRIAVARSLMLRPRILILDEPVSALDLSIQAQILNLLGDLQDEFGLAYIFISHDLSV
                     VRRIARDIIVMYFGKPVEAGPAAEIFNRPRHPYTQALFSATPIADPTRRKQRIRLSGE
                     PPSPLAPPPGCAFAPRCPRAEDKCRQTEPLLEPLDGVDVACFFPVD"
     gene            complement(1427..2284)
                     /locus_tag="VIW27_04050"
     CDS             complement(1427..2284)
                     /locus_tag="VIW27_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019294967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEY5738870.1"
                     /translation="MSRIVYLNGEFLPPEQARVSIFDRAVNFGDAVYEVAGVLDGRLV
                     DFEHHMQRYRGSLQKLGFESPLDREQILAAFRKLVELNDLDEGLVYMQVSRGVAERDF
                     VWPGNLEPTVFMFTQPKSDAERTATETGIRLASTPDIRWARRDIKTVNLLGQVLAKQA
                     AHEAGADEALMVDADGFVTECGSMSFFIVADGRVLTRPLADNGILPGITRRAVIALCA
                     STEMDWCETRFTLEQALTADEAFASAASAYVLPVVNIDDRDIGDGRPGPLTRRLQAIY
                     LDYARASLV"
BASE COUNT          440 a          807 c          779 g          406 t
ORIGIN      
        1 ggttgcaaaa tgataccggc atggcactgg tgctgatcac ccacgatatg ggcgtcgttg
       61 ccgaaacggc gcagcgggtc gtcgtgcaat atgccggcca gcaggtcgaa cggcagccgg
      121 tcggcgggct gttcgaccat ccgcaccatc cctatacggc cgcccttctc gatgcgttgc
      181 cggaccgggc gagcggcgac cggctgccgg cgattcccgg cgttgtgccg ggccaggccg
      241 accggcccga cggctgcctg ttcaatccgc gctgcgcgtt cgccgacgat ttgtgccgca
      301 gcacgcggcc tgcggtgcgc gagggcgacc cgaccctgac cctgtgccat tacccgctgg
      361 cagcgaaacg caccgccgga gcggagtcat gactggcgag ccgatcttgc gggcacacga
      421 gctgacccgc tattacgagg tcaaaaaggg gactttcggc gaatcggcaa cggtcaaggc
      481 gctgaatggc gcatccttcg agctgcgcgc cggcgagacg ctggcgatcg tcggcgaaag
      541 cggttgcggc aagtcgacgc tggcgcgggt gctgacgatg atcgagccgc cgaccagcgg
      601 ccggctggaa atcgagggcc gggagatatc gctggccggc agcaggccat cgaaggaatt
      661 acgccagcgg gtgcagatcg ttttccagaa tccctacggt tcgctgaatc cgcgcaagac
      721 catcgcgacg atgctcgagg aaccgctcaa gctcaacacc gatctcgacc cggcgcaaag
      781 acgccgcgcc gccgaggaaa tgatcgcccg ggtcggcttg cgccccgagc atgccgatcg
      841 ctacccgcac atgttctcgg gcggccagcg gcagcggatc gcggtcgcgc gctcgttgat
      901 gctgcggccc agaatcctga ttctcgacga accggtatcc gcgctagacc tgtccatcca
      961 ggcgcagata ctgaacctgc tcggcgatct gcaggacgaa ttcggcctcg cctatatttt
     1021 catctcacac gacctgtcgg tggtaaggcg catcgccagg gacatcatcg tcatgtattt
     1081 cggcaagccg gtcgaggcgg ggcccgcggc cgagatcttt aaccgcccgc ggcacccgta
     1141 tacccaggcg ctgttttcgg caacgccgat cgccgatccg acgcggcgca agcagcgcat
     1201 tcgcctgagc ggcgaaccgc cgtcgccgtt ggcaccgccg cccggttgcg ccttcgcgcc
     1261 gcgctgtccc cgggcagagg ataaatgtcg gcaaaccgag ccgttgctag aacccctgga
     1321 cggcgtcgac gtggcctgct tcttcccggt cgactgataa tcacccgcac aatgctggta
     1381 ctcaggggcg attgccgctg gcgcgggaat gaccgcgggg ccgggatcag accagtgacg
     1441 cgcgcgcgta gtcgaggtag atcgcctgca gccggcgcgt cagcgggccc ggcctgccgt
     1501 cgccgatatc acgatcgtcg atgtttacca ctggcaggac ataggccgac gcggcgctgg
     1561 caaaggcttc gtctgccgtc agcgcctgct cgagtgtgaa gcgggtttcg caccaatcca
     1621 tttccgtcga tgcacacagc gcgatcaccg cgcggcgcgt gatgccgggc agaatgccgt
     1681 tgtctgccag cggccgggtc aggacgcggc catcggcgac gatgaaaaaa ctcatgctgc
     1741 cgcactcggt gacgaagccg tccgcgtcga ccatcagcgc ctcgtcggcg ccggcctcgt
     1801 gggcggcctg cttcgccagc acctggccga gcaggttgac ggtcttgata tcgcgccgcg
     1861 cccagcgaat gtcgggcgtg ctggcgagcc gaataccggt ctcggtcgcg gttcgctcgg
     1921 cgtcggactt gggctgcgtg aacatgaaca cggtcggttc gaggttacct ggccagacga
     1981 aatcgcgctc ggcgacgccg cgcgacacct gcatgtagac cagcccctcg tcgaggtcgt
     2041 tcaactcgac gagcttgcga aacgctgcca ggatctgttc gcgatcgagc ggcgattcga
     2101 aacccagttt ctgcagcgag ccgcggtagc gctgcatgtg gtgctcgaaa tcgaccaacc
     2161 tgccgtcgag cacgccggcg acctcgtaga ccgcgtcgcc gaaattcacc gcgcggtcga
     2221 agatcgagac cctggcctgc tccggcggca ggaattcacc attcaaatag acgatacggc
     2281 tcatcacgcg gcaccttact ttaacccggc aaaatgctag cataccgcgc atgtcacaaa
     2341 ctcgtcgcga aaaactggcc gcccgctttc gggaattcct cgataccgag agcgtcgtcg
     2401 accaaccgac ctcgctgaag acctacgaat gc
//
LOCUS       DATCZV010000182         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_702160, whole genome shotgun sequence.
ACCESSION   DATCZV010000182 DATCZV010000000
VERSION     DATCZV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702160"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..771)
                     /locus_tag="VIW27_04055"
     CDS             complement(382..771)
                     /locus_tag="VIW27_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738871.1"
                     /translation="MAEQDINHFLEGDGLDTEREKDIGQHIGYRYDVNLVPDMDRCTP
                     FLHNYMEIMGWRDLNWLEDVHMGYEEGRPAVFDRNINGWVTIPSDIELPDNQQDRDMI
                     ARELLVKFQMSKSHPMVDLRKAYEKFA"
     gene            complement(784..2121)
                     /locus_tag="VIW27_04060"
     CDS             complement(784..2121)
                     /locus_tag="VIW27_04060"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY5738872.1"
                     /translation="MSNESDRYNLGKSFIFPPEVINDIHIKSELGRYRMRGFSLFKKI
                     PTWDDLTFMPGTLTRFVIEGYREKCLTKTVIGPEAKRPLELDIPIYVTGMSFGALSYE
                     AKTALARGATMAGTATCSGEGGMIPDERRYSSKWLYQCIQSRYGFNPNHLRLADACEF
                     FIGQGCKVGLGGHLMGQKVTDQVAEMRSLPAGIDQRSPARHPDWLGPDDLALKIQEIR
                     EATDNQIPIQLKLGAARVYDDVRMAAKTGPDSIYMDGMEGGTGAGPHLATEDTGVPGI
                     AAIRQARRALDDVGMSGRISLVYAGGIRNGADVAKAIALGADAVAIGHSVMMALNCNK
                     AIPEADFEKEMGVKAGYCYHCHTGRCPVGVATQDPELRKRLDPDEAAERVYNFLHTLT
                     IEAQMFARACGKTNIHSLEPEDLAALTLEASACAQVPMAGTQYTVGRPDMTRF"
     gene            complement(2149..>2782)
                     /locus_tag="VIW27_04065"
     CDS             complement(2149..>2782)
                     /locus_tag="VIW27_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GXGXG motif-containing protein"
                     /protein_id="HEY5738873.1"
                     /translation="QSGHGSITVRNPRAKHSLGVGILNRLNLTFEGSLGYFGCGLIDG
                     PNVHVTGRVGWSCAENMMAGTVVIEKNAGSLFGAAIRGGDLVCKGQAGARVGIDQKGG
                     TIIVGDSVGAFTGFMMQRGRMIILGDAGDNLGDSMYDGTIYVGGKIASLGVDCVETEL
                     TDLDRKYLRGKLELYGLEAPNGIDNIVKLTSGKQLWNYDNLEPTEKKLVL"
BASE COUNT          504 a          856 c          842 g          580 t
ORIGIN      
        1 atgtggttct tggttctcga tgcgcccacc tcgtacatac agaagccgac gtgaatcaag
       61 aacatgaata ctaccgtgct ccagtagtaa tactcggtaa agataattcc gagcgcagtt
      121 aactcattat ccatcccctc gttacctcgc ttgtttatgt ttattttccg ccgaaattgc
      181 tccggcgagc cccagttacc tcatttccag ttggtgaaaa tgtttaccca cgggaaattt
      241 tctccccccg cgagaaaatc cggttattta tacgcccatt gccggcccgc ttcaagaatt
      301 agccaaaaac gccccatttg agggagtgaa ttgccgattt gccgacaatg cctcagggct
      361 ccgggaaacg ggcccgcggc ttcaggcaaa cttttcgtag gccttgcgca gatcgaccat
      421 cgggtgcgac ttcgacatct ggaacttgac cagcagttcg cgcgcgatca tgtcacgatc
      481 ctgctggttg tcgggtaatt cgatgtcgct cggaatcgtc acccagccgt tgatgttgcg
      541 gtcgaacacc gcgggccggc cttcctcgta acccatatgc acatcctcga gccagttcag
      601 gtcccgccag cccatgatct ccatgtagtt gtgtaaaaac ggcgtacagc gatccatgtc
      661 cgggaccagg ttgacatcgt agcggtaacc gatgtgctgg ccgatatcct tttcgcgttc
      721 ggtatcaaga ccgtcgcctt cgagaaaatg gttgatgtct tgttcagcca tgttgaagcc
      781 ctcctagaaa cgggtcatgt cggggcggcc gacggtgtac tgggtaccgg ccatcggcac
      841 ctgcgcgcag gccgaggcct ccagcgtcag cgcggcgaga tcttccggct cgagcgaatg
      901 aatgttggtc ttgccacagg cgcgcgcaaa catctgcgcc tcgatggtca gcgtgtgcaa
      961 aaagttgtag acccgctcgg cggcctcgtc cgggtccagc cgcttgcgca gttccgggtc
     1021 ctgggtggcg acgccgaccg ggcagcggcc ggtgtggcaa tggtaacagt agcccgcctt
     1081 gacgcccatt tccttttcga aatcggcttc cgggattgcc ttgttgcagt tcagcgccat
     1141 catcaccgag tgaccgatcg cgaccgcgtc ggcgcccagc gcgattgcct tggcaacgtc
     1201 ggcgccattg cggataccgc ccgcgtaaac cagcgaaatg cgtcccgaca tgccgacatc
     1261 gtcgagcgcg cgccgcgcct ggcggatcgc ggcgatgccg ggtacgccgg tatcctcggt
     1321 ggcgaggtgg ggacccgccc cggtgccgcc ttccatgccg tccatgtaaa tcgagtccgg
     1381 cccggtcttg gccgccatgc gcacgtcgtc gtagacccgc gccgcgccga gcttgagctg
     1441 gatcggaatc tggttgtcgg tggcttcgcg aatctcctgg atcttcagcg ccaggtcgtc
     1501 cgggccgagc cagtcgggat ggcgcgccgg cgagcgctgg tcgataccgg ccggcagcga
     1561 gcgcatctcg gcgacctggt cggtcacctt ctgccccatc aggtggccgc cgaggccgac
     1621 cttgcagccc tggccgatga agaactcaca ggcgtcggcg agacgcagat ggttgggatt
     1681 gaagccgtag cgcgactgga tgcactgata cagccacttc gacgaatagc gccgttcgtc
     1741 ggggatcatg ccaccctcgc cggaacaggt cgcggtgccg gccatggtcg cgccgcgtgc
     1801 cagcgcggtc ttggcctcgt aggacaaggc gccgaaggac atgccggtca cgtagatcgg
     1861 gatgtcgagt tccagcgggc gcttggcctc cgggccgata acggttttgg tcaggcactt
     1921 ttcgcgatag ccctcgatga cgaaacgcgt cagggtgccg ggcatgaaag tcaggtcgtc
     1981 ccaggtggga attttcttga acagcgaaaa gccgcgcatg cggtagcggc cgagttccga
     2041 cttgatgtgg atatcgttga tgacctcggg cggaaagatg aagctcttgc ccaggttgta
     2101 gcgatcggat tcgttagaca tctgtattct ccaatttccc ggcggcgcct agagcaccag
     2161 cttcttttcg gtgggctcga ggttgtcgta gttccacaac tgcttgcccg acgtcagctt
     2221 gacgatgttg tcgatgccgt tgggggcttc gagcccgtag agctcgagct tgcccctgag
     2281 gtacttcctg tcgagatcgg tcaactcggt ctcgacacaa tcgacgccga ggctcgcgat
     2341 cttgccgccg acgtatatcg tgccgtcgta catcgagtcg ccgaggttgt cgccagcgtc
     2401 accgaggatg atcatgcggc cacgctgcat catgaagccg gtgaaagcac cgacgctgtc
     2461 gccgacgatg atcgtaccgc ccttctggtc gataccgaca cgcgcgcccg cctgtccctt
     2521 gcagaccagg tcgccaccgc ggatcgcggc gccgaacagc gagccggcgt tcttttcgat
     2581 caccaccgtg ccggccatca tgttctcggc gcaggaccag ccgacacggc cggtgacgtg
     2641 cacgttcggg ccgtcgatca gaccgcagcc gaagtaaccg aggctgcctt cgaaggtcag
     2701 gttgagccgg ttgagaatac ccacgccgag cgaatgcttg gcacgcggat tcctgaccgt
     2761 aatgctgccg tgcccggatt gc
//
LOCUS       DATCZV010000183         8071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_331716, whole genome shotgun sequence.
ACCESSION   DATCZV010000183 DATCZV010000000
VERSION     DATCZV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331716"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1134
                     /locus_tag="VIW27_04070"
                     /pseudo
     CDS             301..1134
                     /locus_tag="VIW27_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
     gene            1330..2367
                     /gene="mutY"
                     /locus_tag="VIW27_04075"
     CDS             1330..2367
                     /gene="mutY"
                     /locus_tag="VIW27_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004854172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5738874.1"
                     /translation="MQEQIAVIDWYRRYGRRDLPWQGRDAYRVWLSEIMLQQTQVATV
                     IPYYLAFVDRFADLRALADADIDEVLQYWQGLGYYARARNLHKAAQIIRDRHRGRFPE
                     DFATLVALPGIGRSTAGAILSLACGQRWPILDGNVKRVLARCFRVPGWYAQSATMKQL
                     WYLAESVTPAENIHEFNQAMMDIGAMVCVRSNPKCEACPLKPMCSSYRHHSQAEFPQR
                     RPARAKPRRQTLMLLHRCGDQLLLWRRPPSGIWGGLWSLPEVDDERAIELWQQSFLSV
                     TQAPKLVQQNVIHHQFTHYSLDISLAIIEMAQLPAKIADRDNYAWVETGELGGRGLPT
                     PVRKLLASLQD"
     gene            complement(2436..2711)
                     /locus_tag="VIW27_04080"
     CDS             complement(2436..2711)
                     /locus_tag="VIW27_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738875.1"
                     /translation="MRKTYEGEMRTRLQALRKNVEALEQRAEQAETNLELEYFTLLEE
                     LRLALENAERKFELLLESHDEQWEGIKTEFEQLWRGARELIRAINSP"
     gene            complement(2752..3102)
                     /locus_tag="VIW27_04085"
     CDS             complement(2752..3102)
                     /locus_tag="VIW27_04085"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HEY5738876.1"
                     /translation="MKTREIAHDEWSEFFDRVSGSLQGKQIVIEVDYPDLGAQIEVSK
                     LSLNGLSYDRKGDAFIISTEVIEHLIHQPRKIFVSGDEDGFDGLEITTDGGGAHIIGF
                     SEPLALPLMPATPA"
     gene            3174..3299
                     /locus_tag="VIW27_04090"
     CDS             3174..3299
                     /locus_tag="VIW27_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738877.1"
                     /translation="MGVKHRCRRAPPATALLDEYQHAVKTGVPPAVAVRADALSR"
     gene            complement(3289..4377)
                     /gene="amrS"
                     /locus_tag="VIW27_04095"
     CDS             complement(3289..4377)
                     /gene="amrS"
                     /locus_tag="VIW27_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY5738878.1"
                     /translation="MPDSHQPDSYQLVETQLQQPLDNGDVICNLCLWRCRLRHGQRGF
                     CQAHVNRNGTLYNLSYGIISAIEVDSIRNKPVYHYRPDSRVMSIGSYGCNFRCKGCHN
                     LEISWGVEALDRLARGQSDDAWVTPRQIVDAAKRHGVEGIAFTYSEPAVWLEYVIDVS
                     RLAHQAGLFTVYVSNSFVTDEALEAMATHIDVLCSDIKSLSDEFYREICPVGRVEQVL
                     GSIRRAAELGIHVETRTNVIPTKNDSLEELAGIAEWIRDNLGAASPWHVTKFFPAYKL
                     SHLPATDDTIIEQAAAAGRALGLENVYAHSEISCDCATENAPVSAWLELDPEAAGKIN
                     KCAADCCGDEGILLKKLEREAGLLGITG"
     gene            complement(4450..6390)
                     /locus_tag="VIW27_04100"
     CDS             complement(4450..6390)
                     /locus_tag="VIW27_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5738879.1"
                     /translation="MSKKDFLKSYVRAMDFKSSLLFVSIGAMIIALTLWNWHYDLKPR
                     LVNEAQSNTRVLAASYARAIESQFQTISGKADVSVIHNSINEMLLINDPTTDENLFLG
                     VTLEVDYDAFPASYDMVNFYVGVTNCENCIVTENPIFSHQSGELIAILKIFANPIFYQ
                     RLTHDIIDDLAVMLFGIVVVLVIAWMTSNRLLSRIRERERSLVFEIGERKSAEEKLHK
                     IAAYDQLTNLPNRYLLQADFKRKLEETARRHNMLATLFFDLDQFKQVNDVHGHETGDA
                     LLEQVASRISDVTRNYDLLARFGGDEFVMIMSNVENRADVIYVAEKIISSFRKSFELP
                     DTSVQITTSIGISIYPDDGRDPSELLKNADLAMYRAKAEGRNCYQFFNSEMNLELQYS
                     QWVENNLRKALEENRLELHYQPQMNLDTGEVESCEALIRWPQEDGDHINPADFIRVAE
                     RTGQIIDISIWVLNEACRNICRWIEDGYQPVRVDINLSSKDFIDRGVLYKFLQILRQY
                     NLKPHNIGIEITENVLLDTTEQVIKSLTVLHNAGVHVSIDDFGTGFSSLNYLKQLPLS
                     GVKIDQSFVHEAPSNKDDLIIMQAITLVGHGFGLDVVAEGVETEWHESLCREIGCNTI
                     QGIFVSEPLTPAEFGGKFLQKG"
     gene            complement(6383..6943)
                     /locus_tag="VIW27_04105"
     CDS             complement(6383..6943)
                     /locus_tag="VIW27_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HEY5738880.1"
                     /translation="MMRSSSGIGLVFALCLLSAAGTPVLAQDPLSPRLQIGINLLPAV
                     IAANNGLAKFTENDSLPIYFVYLENNHIAELLNRSIDRIGEIKQRQLDARAISLEELL
                     ARDIAPMSTIFVAEPLVDRLGELIEYSKARRALLFSPFKGDVDRGVASGFHVTDRVRP
                     LVNLETLKQSKIQLKAFFLRIAVKHE"
     gene            complement(6961..>8071)
                     /locus_tag="VIW27_04110"
     CDS             complement(6961..>8071)
                     /locus_tag="VIW27_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5738881.1"
                     /translation="EWSVSLLQNDTEKNGLFLGVAEAFGGLGDEDDIYADSKLTEQRV
                     EARIDLQYQGEQHTLFGELSVSNVKVAESNQFINLDPVTNLPSPIFNEFPGPVDDMLD
                     RGAVSLVLQDEYRIDSSLTLTSGLRYDNYEDIDTAISPRIALVWRYSDNHVFKTQYAR
                     AFRPPSLIETGGRLEASIDPEINDTVEFGHIYLSTGFTLRNTVFMTRIEDLIVFQDSA
                     PFGYLNAGSHELLGYELEFEKPFAEDWEIVASLSLQDYSDETLPAAAPWMAKLGIGYL
                     LSPSTNLHLQLNSTAARARADGDTRDDFEQTTQADIALRTQNAFNVGGLELRWGLINL
                     LGDEVAYPAPADTYPDDFPTTQGSTLWAQLLFRPL"
BASE COUNT         1523 a         2390 c         2537 g         1621 t
ORIGIN      
        1 gatgccgaca gcggtgctgc cggcggcgaa cgggcggcga gcggcgggcg agatttcgaa
       61 atcgcgggtg ctttcgccgg gctcgatctg caaaacgtca ggctgctgtg gctcgacgag
      121 caggccggca gcagattcga aatcaccgga ctcgacgcca gtaccgggcg catcgtgccc
      181 gaccggccgt tcccgatgac actgcgggtc gatgccagtg ccgacgatgt gttgatcagc
      241 ctcgatttcg cggccgacat cgaatacctg atcggcgagc agcgcctgtc aatgaacaat
      301 atgctgctga cgctcaacga gttcgaaatc agcggcagcc tgcaactgca cgacttctcg
      361 cggccggcgc cggtattgcg cttcgacctg gcgtcccggg atctcgacct cgacgcgctc
      421 accggcaccc tgccggcgca gactgccggt ccggcgccag cgggggagca gggcgatgat
      481 gcgagcgccg gtgaggatct gcgtatccag ctgccgatgc agatcctgcg cgacctggat
      541 atcgacggca agattgccat cgcgcaactc aaggcgcaga acctgcgcat gcaggatctc
      601 gaagtcgcgg tcaaggcgaa ggacggaatc gtcggcctga aaccggtggc gctgaatctc
      661 tacgacggac gggcggagat ctcggtggcc gtcgacgtcc gctcgcaagt cccgaagtat
      721 ggtatcgacg agaccatcag cggcgtacag gcggggccgt tgttgaccga tttcagcggc
      781 gcgcaaacga tcagcggtcg cctggatgcg cagtccaacc tcaccaccag cggcgagtgg
      841 ctcagcgagc tcaagcgcaa cagcaacggc acgctgaaac tcgcgtttac cgacggcgcg
      901 ctgcacggtt tcaatattcg ccagtcgatc gacagcgcga aggccaggct gcgcggtgaa
      961 ccggcgccgc cggcagagac actgaagacc gatttttcgt cgttgtcgct gagcggggtg
     1021 attcgcaacg gggttttctc cagcgacgat ctcgacctgc aggcgccgtt gctgcgcgcc
     1081 ggcggcaagg ggcgcgccga tcttaacgac gaaaccgtcg attacctgat cgacaccaag
     1141 ctggtcggca gtatcgaggg ccagcagggc aagtcggccg atgaactggc cggactggcg
     1201 ataccggtga gcatcaacgg gcctttcagc gggccgaaga tcgacgtatt gctcgatgaa
     1261 atgatgaagg ccagggcgaa cgccgagaag gcccggctga aggcggagat cgaggcgcag
     1321 aaaaaggcct tgcaggagca gatcgcggtg atcgactggt accggcgtta cgggcgccgc
     1381 gatctgccct ggcagggccg ggatgcctac cgcgtctggc tgtccgaaat catgttgcag
     1441 caaacccagg ttgccacagt aatcccctat tacctggctt tcgtcgatcg ctttgccgac
     1501 ctgcgcgcgc ttgccgacgc cgacatcgac gaagtgctgc agtactggca gggcctcggt
     1561 tattacgccc gcgcccgtaa cctgcacaag gcggcgcaaa ttatccgcga tcgacaccgg
     1621 ggccgctttc ccgaggattt cgctacgctg gtagcgctgc ccgggatcgg ccgctccacc
     1681 gccggcgcga ttctcagcct ggcctgcggc cagcgctggc cgatcctcga cggcaacgtc
     1741 aagcgggtgc tggcgcgctg ttttcgcgtg ccgggttggt atgcgcaaag cgcgacgatg
     1801 aagcagctct ggtatctcgc cgagtcggtt acgccggcgg aaaacattca cgaattcaac
     1861 caggcgatga tggacatcgg cgcgatggtc tgcgtcaggt cgaatccgaa atgcgaggcc
     1921 tgcccgctga agcccatgtg ttcgagttac cgtcaccata gccaggcgga attcccgcaa
     1981 cgccggcccg ccagggccaa accgcggcgg caaaccttga tgctgctaca ccgctgcggc
     2041 gatcagctgc tgctttggcg gcggccgccg agcggcatct ggggcgggct gtggtcgttg
     2101 cccgaggttg acgacgagcg cgcgatagaa ctctggcagc agagctttct gtcggttacg
     2161 caggcgccga aactggtgca gcaaaacgtg attcatcacc agttcacgca ttacagcctc
     2221 gatatctcgc tggcgatcat cgagatggcg caattgccgg cgaagattgc cgaccgcgat
     2281 aactacgcct gggtcgagac cggtgaactc ggcggccgcg gtctgccgac cccggtgcgc
     2341 aagctactgg catccctgca ggattagatc cccggtgcca ggcaccgatc gcggctgacc
     2401 gccgagcctt aaccgggcac cggcgggcga gcgattcacg gcgagttgat cgcccttatc
     2461 agctcccgtg ccccgcgcca caactgttcg aactcggtct tgatgccttc ccattgctca
     2521 tcgtgcgatt cgagcagcag ctcgaatttg cgctcggcat tttccagcgc gagccgcagc
     2581 tcttcgagca gcgtgaagta ctcgagctcg agattggttt cggcctgctc ggcgcgctgc
     2641 tcgagggcct cgacattttt ccgcagcgcc tgcaggcgcg tgcgcatctc gccctcgtaa
     2701 gtttttctca tgctgcgtcc tccgccaatt cttgccaccg gcgcggggcc gctaggccgg
     2761 ggtggccggc attaacggca gggccaacgg ctcgctgaag ccgatgatat gcgccccgcc
     2821 gccgtcggtc gttatctcca ggccatcgaa accatcctcg tcgccggaga cgaagatctt
     2881 tcgcggttgg tgaatcaggt gctcgatgac ctcggtagag atgatgaagg catcgccctt
     2941 gcggtcgtag ctcaggccat tgagcgataa cttgctgact tcgatttgtg cgccgaggtc
     3001 ggggtagtcg acctcgataa cgatctgctt gccctgtagc gatccgctaa cccggtcgaa
     3061 gaattcggac cattcgtcgt gtgcaatctc cctggttttc atgatgtgtc tcccgatatc
     3121 ctgccacgtc tctcggaaaa ccaatttaat tcgattgaaa tcgacgcatc ttgatgggag
     3181 tcaagcatcg gtgccgtcgc gcgccgccgg caaccgcgct gctcgacgaa taccagcacg
     3241 cggtgaaaac cggcgtgccg ccggcggtcg cggtgcgggc cgatgcgctc agccggtgat
     3301 gccgagcagg ccggcctcgc gctcgagttt cttcagcagg atgccttcgt cgccgcagca
     3361 gtccgcggca cacttgttga tcttgccggc ggcctcggga tcgagttcca gccacgccga
     3421 tacgggcgcg ttctcggtcg cgcagtcgca ggaaatctcg ctatgcgcgt agacgttctc
     3481 gagacccagc gcgcgaccgg cggcggccgc ctgctcgatg atcgtgtcgt cggtggccgg
     3541 caggtgcgat aatttgtaag cggggaaaaa cttggtcacg tgccacgggc tcgcggcgcc
     3601 gaggttgtcg cgaatccatt cggcgatgcc ggcaagctct tcgaggctgt cgtttttggt
     3661 cggaattacg ttggtgcggg tttcgacgtg tatgccgagt tcggcagccc ggcgaatcga
     3721 ccccagcacc tgctcgacgc ggccgaccgg gcagatctcg cggtaaaatt cgtcggacag
     3781 gctcttgatg tcggagcaca gcacgtcgat gtgggtcgcc atcgcctcga gcgcttcgtc
     3841 ggtgacgaag ctgttggata cgtagaccgt gaacaggccg gcctggtgcg cgaggcgcga
     3901 aacgtcgatc acgtattcca gccacaccgc gggctcggaa taagtgaagg cgatgccttc
     3961 gaccccgtgc cttttcgccg cgtcgacgat ttgtcgcggt gtgacccagg catcgtcgga
     4021 ttgcccgcgc gcgagccgat cgagcgcttc gactccccag gagatttcca gattgtggca
     4081 acccttgcag cggaagttgc aaccatagga accgatcgac atgacacggc tgtcgggccg
     4141 gtagtggtag accggcttgt tgcgaatcga atccacctcg atcgccgaaa tgatgccgta
     4201 ggaaaggttg taaagcgtgc cgttacggtt tacgtgcgcc tggcaaaaac cgcgctggcc
     4261 atgccgcagc cggcagcgcc acaggcacag gttgcagatc acgtcgccgt tgtcgagcgg
     4321 ctgctgcagc tgcgtctcga cgagctggta gctatcgggc tgatgggaat cgggcatcgg
     4381 agggtttcga gtttggtgtc cgctgcaatg tagcctaatt ccgctcgcgg cgcaaagccc
     4441 gcgaccggat cagcctttct gcagaaactt gccgccgaat tccgccggcg tcagcggctc
     4501 ggaaacgaat atcccctgta tcgtgttgca gccgatttcg cggcacagcg attcgtgcca
     4561 ctcggtctcg acaccctcgg cgaccacgtc gaggccgaag ccgtgtccga ccagtgtgat
     4621 cgcctgcatg atgatcaggt catccttgtt gctcggcgcc tcgtgcacga agctctgatc
     4681 gatcttgacg cccgataacg gcaattgctt gaggtagttc agcgacgaga atccggtgcc
     4741 gaagtcgtcg atcgaaacgt gcaccccggc gttatgcagt accgtgagcg acttgatcac
     4801 ctgctcggtg gtatcgagca gcacgttctc ggtgatctcg ataccgatgt tatgcggctt
     4861 caggttgtac tgccgcagta tctgcaaaaa cttgtagagc acgccgcgat cgatgaaatc
     4921 cttgctcgac aggttgatgt cgacacgcac cggctggtaa ccgtcctcga tccagcggca
     4981 gatattgcga caggcctcgt tgagtaccca gatgctgata tcgatgattt gcccggtacg
     5041 ctcggcgacg cgaataaaat cggcggggtt gatgtgatcg ccgtcttcct gcggccagcg
     5101 aatcaacgcc tcacaggatt cgacctcgcc ggtatcgagg ttcatctgcg gctggtagtg
     5161 cagctccagc cggttttcct cgagcgcctt gcgcaggttg ttctcgaccc attgcgagta
     5221 ttgcagctcg aggttcatct ccgagttgaa gaactggtag cagttgcgtc cttcggcttt
     5281 ggcgcgatac atcgccaggt cggcgttttt cagcagctcg gatggatccc tgccatcgtc
     5341 gggatagatg ctgatgccga tgctggtggt gatctggacc gaggtatcgg gaagttcgaa
     5401 ggacttgcga aaactggaaa tgatcttctc ggcgacgtag atgacatccg cgcgattctc
     5461 gacgttcgac atgatcatga cgaattcgtc accgccgaaa cgcgccagca aatcgtaatt
     5521 gcgggttaca tcggagattc gcgaggcgac ctgctcgagc agcgcgtcgc cggtttcgtg
     5581 gccgtggacg tcgttgacct gcttgaattg gtcgaggtcg aaaaacagcg tcgccagcat
     5641 gttgtgccgg cgcgcggttt cctccagctt gcgcttgaaa tcggcctgta gaaggtagcg
     5701 attgggcaag ttggtcagct ggtcgtaggc cgcgatcttg tgcagttttt cttccgccga
     5761 cttgcgctcg ccgatttcga ataccaggct gcgttcgcgc tcgcgaatgc ggctcagcag
     5821 gcggttggaa gtcatccacg cgataaccag caccaccacg ataccgaaca gcatcaccgc
     5881 gaggtcgtcg atgatgtcat gagtcaggcg ctggtagaaa atcgggttgg cgaaaatttt
     5941 caggattgcg atcaactcgc cgctctggtg gctgaagatc gggttttcgg taacgatgca
     6001 gttctcgcaa ttggtgaccc cgacgtagaa attgaccatg tcgtaagaag ccggaaaagc
     6061 gtcgtagtcg acttccagcg tgactcccag gaacaggttt tcatcggtgg tcggatcgtt
     6121 gatcaacagc atctcgttga tcgagttatg gattaccgag acatcggcct tgccgctgat
     6181 ggtctggaac tgcgattcga ttgcccgcgc ataggatgcg gccagcacac gggtattcga
     6241 ctgggcctcg ttgaccagtc gcggcttgag atcgtagtgc cagttccaca gcgtcagtgc
     6301 gatgatcatc gcgccaatac tgacgaatag cagcgaggac ttgaaatcca tcgcccgaac
     6361 atagctcttg agaaaatctt tcttactcat gtttcaccgc gatgcgcaga aaaaacgctt
     6421 tcaattgaat tttggattgc ttgagagttt cgaggtttac cagtggccgc acgcggtcgg
     6481 tgacgtggaa accggaagcc acacccctgt cgacatcccc cttgaacggg gaaaacaaca
     6541 gtgctcgacg agctttcgaa tactcgatca gttcgccgag ccgatccacc agcggctcgg
     6601 ccacaaaaat cgtactcatc ggcgcgatat cccgcgccag cagttcctcg agcgatatcg
     6661 cgcgggcatc cagctggcgc tgcttgattt cgccgatgcg gtcgatgctg cggttcagca
     6721 actcggcaat gtggttgttt tcgaggtaga cgaaataaat cggcaggctg tcgttttcgg
     6781 tgaacttggc gagcccgttg ttggcggcga tcaccgccgg cagcaggttg atcccgattt
     6841 gcaggcgcgg cgacaacggg tcctgcgcga gcaccggtgt gccggcggcc gacaacaagc
     6901 acagggcgaa aaccagcccg atacccgacg atgagcgcat catgggtcgc cgcccacggt
     6961 tcatagcggc cggaacagca gttgtgccca cagcgtgctg ccctgggtgg tcggaaagtc
     7021 gtccgggtag gtgtcggccg gagccgggta ggcgacctcg tcgcccagca ggttgatcag
     7081 gccccagcgc aattccaggc caccgacgtt gaaggcgttt tgtgtacgca gcgcgatgtc
     7141 ggcctgcgtg gtctgctcga agtcgtcgcg cgtatcgccg tccgcgcgcg cgcgcgcggc
     7201 ggtgctgttg agttgcagat gcaggttggt cgacggcgag agcaggtaac cgataccgag
     7261 cttcgccatc cacggcgccg cggccggcag ggtctcgtcg gagtaatcct gcagtgacag
     7321 gctggcgact atttcccagt cctcggcgaa cggcttctcg aactcgagtt cgtagccgag
     7381 taattcatgc gagcccgcgt tgaggtagcc gaacggcgcg ctatcctgga acacgatcag
     7441 gtcttcgatg cgggtcatga agaccgtgtt gcgcagcgta aacccggttg acagatagat
     7501 atgcccgaac tcgacggtat cgttgatttc aggatcgatc gacgcttcca gccggccccc
     7561 ggtctcgatc agcgacggcg gccgaaaggc gcgcgcatac tgggtcttga agacatggtt
     7621 gtcggagtat cgccagacca gcgcgatgcg cggtgaaatc gcggtgtcta tatcctcgta
     7681 attgtcgtag cgcagtcccg aggtcagggt caggctgctg tcgatgcggt actcgtcctg
     7741 cagcaccagg ctgaccgcgc cacgatcgag catgtcgtcg accggcccgg ggaactcgtt
     7801 gaatatcggc gacggcaaat tggtgaccgg atcgaggttg atgaactgat tcgattcggc
     7861 aaccttgacg ttgctgaccg acagttcgcc gaacagcgtg tgctgttcgc cctggtattg
     7921 caggtcgatg cgggcctcga cacgctgctc ggtcagtttc gagtcggcgt agatgtcatc
     7981 ctcgtcgccg aggccgccaa aggcctccgc cacgccgagg aacaggccgt ttttctcggt
     8041 atcgttttgc agcagactga ccgaccactc g
//
LOCUS       DATCZV010000184         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_490194, whole genome shotgun sequence.
ACCESSION   DATCZV010000184 DATCZV010000000
VERSION     DATCZV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490194"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VIW27_04115"
     CDS             complement(<1..298)
                     /locus_tag="VIW27_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5738882.1"
                     /translation="MSDIDEMRRRRTFAIISHPDAGKTTVTEKLLLFGKAIQMAGTVK
                     SRKAARHATSDWMEMEKQRGISVTSSVMQFPYRDSIINLLDTPGHEDFSEDTYRT"
     gene            complement(606..1085)
                     /gene="rimI"
                     /locus_tag="VIW27_04120"
     CDS             complement(606..1085)
                     /gene="rimI"
                     /locus_tag="VIW27_04120"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY5738883.1"
                     /translation="MSESPLRFRRMLLSDIDRVMKVEREVYDFPWTERIFSDCIRVGY
                     LCWLALRDQDVVGHAIISVVAGESHMLNLSIARAHQGRGYGRQFIDFLVSEARARNAQ
                     TMLLEVRPSNFAAINCYNSAGFNEIGSRKDYYPAADGREDALLFAKQLASSSESASG"
     gene            complement(1082..1882)
                     /locus_tag="VIW27_04125"
     CDS             complement(1082..1882)
                     /locus_tag="VIW27_04125"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5738884.1"
                     /translation="MLTARQQHCLRGIGVPLWRARAGAAVDAPAAEHETSLDTVAAAQ
                     AAAEPQAPLAVAAAAPPADETLSAVAAQPAPVVPVATTKLDSWDAILTAIHACQACEL
                     AQNCTQKVPGVGDRQADLLIVGEGPGHDEDIQGEPFVGRSGQLLDRMLAAIGFSREQV
                     YITNIVKCRPPNNRDPRPDEAQHCRAYLEAQIERIAPRAILSVGRVSAHNLLGSDQPV
                     GKLIRQQHTLPGTDIPVKVTYHPAYLLRNPSAKAIAWQDLKLLHRMLR"
     gene            complement(1889..>3411)
                     /locus_tag="VIW27_04130"
     CDS             complement(1889..>3411)
                     /locus_tag="VIW27_04130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5738885.1"
                     /translation="DTTLRDGEHSPGASMTRDEKIRIARALELMKVDIIEAGFPIASD
                     GDFESVKAVAEIIKDSTVCGLARAKPVDIERAGEAIKPANSGRIHTFLATSPIHMEHK
                     LRMTPDQVVESAVAAVRKARQYTDDVEFSPEDAGRSEPDFLCRVIEAVIAEGATTINI
                     PDTVGYNVPHQFGSLIRQLIERIPNADKAVFSVHCHNDLGLAVANSLEAVLNGARQVE
                     CTINGLGERAGNASLEEIVMAVRTRQDIFPCETGLDSTQIMNCSKLVSGITGFTVQPN
                     KAIVGANAFAHESGIHQDGVLKSRETYEIMRAQDVGWSTNKLVLGKHSGRNAFKTRMS
                     ELNIHFDSEEELNEVFAAFKALADKKHDIYDEDLLALVNEVAFETENETVKLVSLRVC
                     CETGETPVASITLNIDGEEQRSEASGDGPVDACFKAIEKLTATGVNLQLYSVNAITGG
                     TDSQGEVTVRVEKQGRVINGQGADTDIVIASAKSYINAINKVINSSGRTHPQLGDV"
BASE COUNT          574 a         1049 c         1098 g          690 t
ORIGIN      
        1 gcgtgcggta agtgtcctcc gagaaatcct cgtgaccggg cgtgtccagc aggttgatga
       61 tcgagtcgcg gtaggggaac tgcatcaccg aggaggttac cgagatgccg cgctgtttct
      121 ccatctccat ccagtcggag gtcgcatgcc gcgcggcctt gcggctcttg acggtacccg
      181 ccatctggat cgccttgccg aacagcagca gtttttcggt gaccgtggtc ttgcccgcgt
      241 cgggatgcga gatgatcgca aaggtccggc gacgacgcat ttcgtcaata tcagacatac
      301 tagaattcca ggatcagaca gggtagggcg gctagcccga gcgttgtgcc ggcagtgcgc
      361 gcgataatac ggcccaaccg cggccgggcg atcgatccgg ccataggccg cggctcgatg
      421 gcaggccgcg cgcggcggcc ggctcgaaca tggttcggca ggccgggttc gaaatggccg
      481 cggcgcgcag gcgcctgcgg ttagcgttat gcgctctcaa tataccctct gtcatgcgtt
      541 caaatactat tcgacttcat cgccatatgg cggggtcggc gaccaccgtt ggctgcaatg
      601 acaggctagc cgctggcgga ttcggacgac gacgcgagct gcttcgcgaa cagtaacgcg
      661 tcttccctgc catcggcggc gggataataa tccttacggc tgccgatctc gttgaaaccc
      721 gcggaattat aacaatttat tgccgcgaaa ttgctcgggc gaacctcgag cagcatggtt
      781 tgcgcgttac gcgcgcgtgc ctcgctgacc aggaagtcga taaattggcg cccgtatccg
      841 cggccctggt gcgcgcgtgc gatcgacagg ttgagcatgt ggctttcgcc ggcgaccacg
      901 ctgatgatcg cgtgaccgac gacatcctgg tcgcgcagcg ccagccagca caggtaaccg
      961 acgcgaatgc agtcgctgaa gatacgctcg gtccacggga aatcatagac ctcgcgttcg
     1021 actttcatga cgcgatcgat atccgagagc agcatgcgcc ggaacctgag tggggattcg
     1081 ctcatcgaag cattcgatgc agcaatttca ggtcctgcca ggcgatggcc ttggctgacg
     1141 gattacgcaa caggtaggcg gggtggtagg tgaccttcac cggtatatcg gtgcccggca
     1201 gcgtatgctg ctggcgaatc aacttgccca ccggctgatc gctgccgagc aggttgtgtg
     1261 cggatacgcg gccgaccgac aggatcgccc gcggcgcgat gcgttcgatt tgcgcctcca
     1321 ggtaggcgcg gcaatgctgc gcctcgtccg gccgcggatc gcgattattg ggcggccggc
     1381 acttgacgat attggtgatg taaacctgct cgcggctgaa gccgatggcg gccagcatgc
     1441 ggtcgagcaa ctggccggaa cgcccgacga agggctcgcc ctgaatatcc tcgtcgtggc
     1501 cgggaccttc gccgacaatc agcagatccg cctgccggtc gccgaccccg ggcaccttct
     1561 gggtacagtt ttgcgccagc tcgcaggcct ggcaggcatg gatcgcggtg aggatagcat
     1621 cccaggaatc gagcttcgtg gtcgccacgg gaacgaccgg cgccggctgc gcggcgaccg
     1681 cgcttaaggt ttcgtcggcg ggcggcgctg ccgccgctac cgccaacggc gcctgcggct
     1741 ccgcggcggc ttgcgcggcg gccactgtat cgagggaggt ttcgtgctct gcggccggcg
     1801 catcgacggc cgcgccagcg cgcgctcgcc acaggggaac accgattccc cgcaggcagt
     1861 gctgttggcg cgcggtcagc atcggtacct acacgtctcc cagttgcggg tgggttctgc
     1921 cggagctgtt gatgaccttg ttgattgcgt tgatatagga ttttgccgac gcgatcacga
     1981 tgtcggtgtc ggcgccctga ccgttgatca cccggccctg cttctcgacg cgcacggtga
     2041 cctcgccctg cgaatcggta ccgccggtaa tcgcgttaac cgagtaaagc tgcaggttga
     2101 cgccggtagc ggtcagtttt tcgatcgcct tgaaacaggc atcgaccggt ccgtcgccgc
     2161 tggcttcgga gcgctgctcc tcaccgtcga tgttgagcgt gatgctggcc accggcgtct
     2221 cgccggtctc gcagcaaacc ctgaggctca ccagcttgac ggtctcgttc tcggtttcga
     2281 aggcgacctc gttgaccagt gccagcaagt cttcgtcgta gatatcgtgt ttcttgtcgg
     2341 ccagtgcctt gaatgccgcg aatacctcgt tcaattcctc ctccgaatcg aagtggatat
     2401 tcaactcgga catgcgggtc ttgaacgcgt tgcggcccga atgcttgccg agcaccagct
     2461 tgttggtact ccagccgacg tcctgggcgc gcatgatttc gtaggtttcg cgcgatttca
     2521 gcaccccgtc ctgatgaatg ccggattcgt gggcgaaagc gttggcgccg acgatcgcct
     2581 tgttcggctg cacggtgaac ccggtgatcc cggaaaccag cttcgagcag ttcatgatct
     2641 gcgtgctatc gaggccggtt tcgcagggaa agatatcctg gcgggtgcgt accgccatca
     2701 cgatttcttc gagcgatgcg ttgccggcac gttcaccgag gccgttgatc gtgcactcca
     2761 cctggcgtgc gccgttcaac acggcttcca gcgagttggc caccgcgagc ccgaggtcgt
     2821 tgtggcagtg caccgagaat accgccttgt cggcgttcgg aatacgttcg atcagctgcc
     2881 gaatcagcga accgaactgg tgcggcacgt tgtagccgac ggtatccggt atgttgatcg
     2941 tcgtcgcacc ctcggcgatc accgcctcga tgacccggca caggaaatcg ggttccgagc
     3001 gtcccgcgtc ctcgggcgaa aactcgacgt cgtcggtgta ctgccgcgcc ttgcgaacgg
     3061 cggccaccgc ggactcgacg acctggtcgg gcgtcatccg cagcttgtgc tccatgtgga
     3121 tcggcgaggt cgcgagaaag gtgtgaatgc gccccgagtt ggccggcttg atggcctccc
     3181 ccgcgcgctc gatatcgacc ggcttggcgc gcgccaggcc gcaaacggtg ctgtccttga
     3241 tgatttcggc gaccgctttc accgattcga agtcgccgtc gctggcaatc gggaaaccgg
     3301 cctcgatgat gtcgaccttc atcagctcga gggcgcgggc gatgcggatt ttctcgtcgc
     3361 gcgtcatcga tgcgcctggg ctatgctcac cgtcgcgaag cgtggtgtcg a
//
LOCUS       DATCZV010000185         7700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_596543, whole genome shotgun sequence.
ACCESSION   DATCZV010000185 DATCZV010000000
VERSION     DATCZV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596543"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VIW27_04135"
     CDS             <1..1118
                     /locus_tag="VIW27_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5738886.1"
                     /translation="RFIFERNPYYHRIDENGRQLPYLDSIAVNIASSKLVPAKAGADE
                     SDLQARYLRMDNYTFLKTAARHSNYDVRLWQTAKGAHMALYPNLNASDPVYRELLQDV
                     RFRRALSLAIHRHEVNQVVYFGLVGEGNNTVLMESPLYRPEYQRDWAEFDLRTANRLL
                     DELGLVERDDRGVRLMADGRPLEILVQTAGESTEQTDVLELIHDSWLEAGIKLYSIPS
                     TREVFRNRIFSGDAIMSIWSGLENGIPGVSSIPAELAPTNKYQYHWPQWGAWYETRGK
                     SGQPPELPAARELVELVDRWKQAIGDAERREIWHRMLDIHREQVFTIGIVNRVPHPVV
                     VNNHLHNVPETGFYNFDPGAYFGIYRPDTFWFDEARR"
     gene            1118..2116
                     /locus_tag="VIW27_04140"
     CDS             1118..2116
                     /locus_tag="VIW27_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738887.1"
                     /translation="MLAYILRRILTMIPTLLIISLLVFVIIQLPPGDYLESHIAELQS
                     QGESVDEKKIQFLREQYGLDKPLPQQYWYWLTGMLQGDFGYSFEYDLPVTDVVGDRLF
                     LTVLISIATILFTWAVAFPIGIYSATHQYSWGDYGLSTLGFLGLATPNFLLALVMLYL
                     ANVYFGTSIGGLMDAEYVDAPWSLDKLLSILEHLWIPVIVIGTSGTAGMIRRLRANLL
                     DELQKQYVTTGRAKGLPPGRLLRKYPLRIAINFFVADIGNLLPTVISGAEIVAVVMSL
                     PTTGPILLASLQSQDMYLAGSFLMFLALLTVVGVLVSDILLALLDPRIRLGGSAGK"
     gene            2113..3267
                     /locus_tag="VIW27_04145"
     CDS             2113..3267
                     /locus_tag="VIW27_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738888.1"
                     /translation="MTHEHFVSSEPFDPRSIEALTPAQEKYYLASQWKLMWWKLKRHR
                     LAVICGVILALNYATVLFSEVIAPYDLHTRHTDHIYSPPHSVHLFHDGEFIGPFVYAR
                     DYELDMEILKRNYPERRGRQDVQPLRFFCLGDEYRFWGLFEASFHLVCPPDNGTFFLL
                     GTDRLGRDMFSRITYGARISLTIGLVGITISFFFGIVIGGLAGYYGGWVDASVQRLIE
                     VIRSFPELPLWMALSAVLPVNWSPILIFFGITVILALLDWTGLARAVRSKLLALREED
                     YCAAAELMGARPGRIIGRHLLPGFMSHLIASATLSVPAMILGETALSFLGLGLRPPVT
                     SWGVLLNEAQNINVVALYPWLLFPVVPVVLVILAFNFFGDGLRDAADPYK"
     gene            3271..4155
                     /locus_tag="VIW27_04150"
     CDS             3271..4155
                     /locus_tag="VIW27_04150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5738889.1"
                     /translation="MQPGFQARDRCPVCDARKRRELCAIRFDDPRMAEFLDGFYRGRL
                     PLDALHGGVYRVVACGRCQFVFQDPVLDDSGMHLLYEHWIDDDRSLRKKQSAEQTLKR
                     RYAGQLQSVAALLHGSPATQRLLEFGMGWGYWCRAAHEYGFDVSGFELSAARRAYARE
                     MGVRVIDELPPPGARFDCIYSSQVFEHLPEPRQTLRALCARLAPGGLVYLRVPDGRGV
                     AQTLTTRGWSPGMQAIHPLEHINCFTRRTLIALAASQGLRRVDPPPRLAWRSPWRSLK
                     REISDRYLNTHLFFRLVD"
     gene            complement(4165..6798)
                     /locus_tag="VIW27_04155"
     CDS             complement(4165..6798)
                     /locus_tag="VIW27_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY5738890.1"
                     /translation="MEPSLYRYILTHSKRDQLLLVLLSLASLPLVYITLELPKKIINL
                     LEGVELPDQLLGFELDRLEFLMALSFAFLLVVLSSGGLKYLLNVYRGALGERILRRFR
                     YELYERILRFPLPHFKRVSSGELIPMITAETEPLGEFIGESFTLPVFQGGTLLTYLFF
                     IFQQDLYLGLAAIALYPFQLYVIPRLQRRVNELSKERVSTVRNLSGRIGETISGIGDV
                     HANDTSRFERAHISQRLGRIYLIRFDIYKRKFFIKFLNNFIAQLTPFFFYSVGGYFVL
                     RGDLSIGSMVAVLVAYKDLSGPWKELLRYYQRMEDVKVKYTQVIQQFDPHNLIEPEII
                     NERPESVALPGQEIRANNLRYSEDGLYFSIDGASFKLPVDQHCAAVGLGNSGKDELAG
                     LVSRLLHPSSGNLILGNAKMQELPEAITGNRLGYVGSSPFMFNGSVFDNLTYSLKHQP
                     VKSADQKPDKALEQERHLAALAGNTPDRYDDEWIDYQALDCADREQLIERIVETLKCV
                     ELDEEIYQFGLQSFVDQSKHADFAARIMEARRQLRDRFTDSAVEKLVEPFDHRKYNLN
                     MTVAENLLFGTIYDEQIDTDSLIDNPVIGEVLDETGLSETFLEAGAKITEIMLDLFSD
                     VEPDSELFEQFSFISAEDLPDFNRLLQVTRQRGIGDLNTADKHRLIALTFKLVVARHR
                     LGLITEPIQQRILEARQRIYELAQSEDLGIEFFNFESFNPRISIQDNILFGKLAYGQA
                     FAQQKINALIADLVQSLNLREDIIEAGLGHDVGVAGGRLTSSQRQKLGVARALLKKPD
                     LLIVNEALSSLDTTAERRLIENIRQAMDGRGILWILGRVQLAEQFDNILVMERGKLVE
                     QGSYDHIHSTNEHFQHLLQAE"
     gene            6946..>7700
                     /locus_tag="VIW27_04160"
     CDS             6946..>7700
                     /locus_tag="VIW27_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HEY5738891.1"
                     /translation="MKGYPRLSETFVAQEIRALELRGFELCIFSLRHPYDPAIHPVHA
                     EIEAPVYYLPEYAHDDWPRVLSACLRLCLRAGFWRALVRLAADLLHDPTRNRLRRFAQ
                     AAVMSVEMPATVRHYYVHFMHTPASVGYYSSLISGRSWSISAHAKDIWTLDRRELAAK
                     LASADWTVTCTAANRRYLAQIADRDDKVALLYHGLDFSRFELNPGHPAQRDGRDPTHP
                     VRLISVGRAVDKKGYDDLLRALAELPADLHWRFT"
BASE COUNT         1402 a         2303 c         2405 g         1590 t
ORIGIN      
        1 agcgctttat cttcgagcgt aatccctact accaccggat cgacgagaat ggccggcaat
       61 tgccttacct cgactcgatc gcggtcaata tcgcttccag caagctggtg ccggcgaagg
      121 ccggcgccga cgagtcggac ctgcaggcgc gctacctgcg catggacaac tacacgtttc
      181 tgaagaccgc ggccaggcac agcaattacg acgtgcgcct gtggcagacc gccaagggtg
      241 cccacatggc gctgtatccg aacctgaacg ccagcgaccc ggtgtaccgc gagctgctgc
      301 aggacgtacg tttccgtcgc gcgctgtcgc tggcgatcca tcggcacgaa gtcaaccagg
      361 tggtgtactt cggtctggtc ggcgaaggca ataacacggt attgatggag agcccgctgt
      421 atcggccgga atatcagcgc gactgggccg aattcgacct gcggaccgcc aaccgcctgc
      481 tcgatgaact cggcctggtc gagcgtgacg atcgcggcgt tcgcctgatg gcagacggca
      541 ggccgctgga gatcctggtg cagaccgccg gcgagagcac cgagcagacc gacgtactgg
      601 aattgatcca cgacagctgg ctcgaggccg gcatcaagct gtacagtatt ccatcgaccc
      661 gcgaagtgtt ccgcaaccga atattttccg gcgacgcgat catgtcgatc tggtccgggc
      721 tcgaaaacgg gattcccggc gtgtccagca ttccggcgga actcgcgccg accaacaagt
      781 accagtacca ctggccgcag tggggcgcct ggtacgagac tcgcggcaaa agcggtcagc
      841 cgcccgagct gccggcggca cgcgaactgg tggaactcgt cgatcgctgg aaacaggcca
      901 tcggtgacgc cgagcgcagg gagatctggc atcgcatgct ggatatccac cgcgagcagg
      961 tgtttaccat cggcatcgtc aatcgcgtgc cgcacccggt agtcgtcaac aaccatctgc
     1021 ataacgtgcc cgaaaccggc ttctacaatt tcgatccggg cgcctatttc ggcatttacc
     1081 ggccggatac cttctggttc gacgaggcca ggcgttaatg ctcgcctaca tcctgcgccg
     1141 cattctgacc atgataccga cgttgctgat catcagcctg ctggtgttcg tcatcatcca
     1201 gttgccgccg ggggattacc tcgagagcca tatcgccgaa ctgcaaagcc agggagagtc
     1261 ggtcgacgaa aagaaaatcc agttcctgcg cgagcaatac gggctcgaca agccgctgcc
     1321 gcaacagtac tggtactggc tgaccggcat gctgcagggg gatttcggtt attccttcga
     1381 atacgacctg ccggtcaccg atgtggtcgg cgaccgcctg ttcctgaccg tgctgatctc
     1441 gatcgcgacg attctgttca cctgggcggt ggccttcccg atcggtattt actcggccac
     1501 gcatcaatac agctggggtg attacgggct gtcgacgctc ggttttctag gcctggcgac
     1561 accgaacttc ctgctcgcgc tggtcatgct gtacctcgcc aacgtttact tcggcaccag
     1621 catcggcggc ctgatggacg ccgaatacgt cgatgcgccc tggagcctcg acaagctgct
     1681 gtcgatcctc gagcacctgt ggatcccggt catcgtcatc ggcacctcgg gcaccgcggg
     1741 catgatccgt cggttacgcg ccaatctgct cgacgaactg caaaagcagt atgtgaccac
     1801 cgggcgcgcc aaggggctgc cgccgggcag gttgctgcgc aagtacccgc tgaggatcgc
     1861 gatcaatttt ttcgtcgccg atatcggtaa cctgctgccg acggtgatat ccggtgccga
     1921 gatcgtcgcc gttgtcatgt cgttgccgac gaccgggccg atcctgctcg cctcgttgca
     1981 aagccaggac atgtacctgg cgggatcctt cctgatgttc ctcgccctgc tgacggtggt
     2041 cggcgtgctg gtctcggata tcctgctggc cctgctggat ccgcgtattc gcctcggcgg
     2101 gagcgcgggt aaatgacgca cgaacatttc gtttccagcg aacccttcga tccgcgctcg
     2161 atagaggcgc tgaccccggc gcaggaaaaa tactacctgg cgtcgcaatg gaagctgatg
     2221 tggtggaagc tcaagcgtca ccggctggcg gtgatctgcg gcgtcatcct ggcactgaac
     2281 tacgcgacgg tcctgttcag cgaggtaatc gcgccctacg acctgcacac ccggcacacc
     2341 gaccacatct attcgccgcc gcatagcgtg cacctgtttc acgacggcga gttcatcggg
     2401 ccgttcgtct atgcccgcga ctacgagctg gacatggaga tactgaagcg caattacccc
     2461 gagcgcaggg ggcggcaaga cgtgcagccg ctgcgctttt tctgcctggg agacgagtat
     2521 cgtttctggg ggctgttcga ggccagcttc catctggtct gcccacccga caacggcacc
     2581 tttttcctgc tcggcaccga ccgcctgggt cgcgatatgt tctcgcggat tacctacggc
     2641 gcgcgcatat cgctgaccat cgggctggtg ggtatcacga tcagcttttt cttcggcatc
     2701 gttatcggcg gcctggccgg ctattacggc ggctgggttg acgcctcggt acagcgcctg
     2761 atcgaggtca ttcgctcgtt tccggagctg cctttgtgga tggcgctgtc ggcggtgcta
     2821 ccggtcaact ggagcccaat actaatcttt ttcggcatca ccgtaatcct cgcgctgctc
     2881 gactggaccg ggctggcacg tgccgtgcgc tcgaaactgc tggcgctgcg cgaagaggat
     2941 tactgtgccg cggccgagct gatgggcgcg cgtccgggac gcatcatcgg acgccacctg
     3001 ctgcccgggt tcatgagcca cctgatcgcg tcggcgacgc tgtcggtgcc ggcgatgatt
     3061 ctcggcgaaa ctgcgctcag ctttctcggc ctcggcctgc ggccgccggt gaccagctgg
     3121 ggggtattgt tgaacgaggc gcagaacatc aatgtcgtgg cgctttatcc gtggctgttg
     3181 tttccggtgg tcccggtggt gctggtgatc ctggccttca actttttcgg cgacggattg
     3241 cgcgacgctg ccgatcctta caagtaaccg atgcagcccg gtttccaggc gcgcgatcgc
     3301 tgcccggtct gcgacgcgcg caagcgccgc gagttgtgcg cgattcgctt cgacgatccg
     3361 cgcatggcgg aattcctcga cgggttttat cgaggccggt tgccgctcga tgcgctgcac
     3421 ggcggagttt accgggtagt cgcctgcggg cgctgccaat tcgtgttcca ggatccggtg
     3481 ctcgatgaca gcggtatgca tctgctatac gagcactgga tcgatgatga ccgcagcctg
     3541 cgcaagaaac agtccgccga gcaaaccctg aagcgtcgct atgcaggtca gctgcagtcg
     3601 gtggcggcgc tgctgcacgg atcgccggcg acgcagcggc tgctcgaatt cggcatgggc
     3661 tgggggtact ggtgccgcgc cgcgcacgaa tacggtttcg acgtcagcgg tttcgaactg
     3721 tcggcggcgc gccgcgctta cgcgcgcgaa atgggtgtca gggtaatcga cgaattgcct
     3781 ccgcccgggg cgcgcttcga ttgcatctat tcgagccagg tgttcgagca cctgccggag
     3841 ccgcggcaga ctctgcgcgc gctgtgcgca cggctggctc cgggcgggct cgtctacctg
     3901 cgcgtgccgg acggtcgcgg cgtcgcacaa acgttgacta cccggggctg gtcacccggc
     3961 atgcaggcga tccacccgct cgaacacatc aactgtttca cccgccggac actgatcgcg
     4021 ctggcggcat cgcaggggct gcgacgcgtc gatccaccac cgcggctggc atggcgcagc
     4081 ccgtggcgca gcctgaagcg ggagatttcc gatcgttacc tgaataccca cctgttcttc
     4141 aggttagtcg actgatatcc gtgcttactc cgcttgcagc agatgctgga agtgttcgtt
     4201 ggtcgagtgg atgtggtcgt aggatccctg ttcgaccagc ttgccgcgct ccatgaccag
     4261 gatgttgtcg aattgctcgg ccagttgcac gcggccgaga atccataaaa tgccgcgccc
     4321 gtccatggcc tggcgaatgt tttcgatcag gcggcgctcg gcggtggtat cgagactgga
     4381 cagcgcctcg ttgacgatca gcagatcggg ttttttcagc agcgcgcgcg ccacgccgag
     4441 cttttgtcgc tggctggacg tcaggcgtcc cccggccacg ccgacatcgt ggccgagacc
     4501 ggcctcgatg atgtcctcgc gcaggttgag cgactgcacc aggtcggcga tcagcgcatt
     4561 gattttttgc tgcgcgaaag cctgcccgta agccagcttg ccgaacagga tattgtcctg
     4621 gatcgagatg cgcggattga aggattcgaa gttgaagaat tcgattccca ggtcctcgct
     4681 ttgcgcgagt tcgtaaatac gctggcgggc ctcgagaatg cgctgctgga ttggctcggt
     4741 aatcagtccg aggcgatgcc gcgcgaccac cagtttgaat gtcagggcta tcaggcgatg
     4801 cttgtcggcg gtattcaggt cgccgatacc acgctgtcgc gtaacctgca gcaggcggtt
     4861 gaagtcgggc aggtcttccg cggaaatgaa gctgaattgt tcgaacagct cgctgtcggg
     4921 ttcgacgtcg gaaaacaggt cgagcatgat ctcggtgatt ttcgcgccgg cctcgagaaa
     4981 ggtttcgctg agcccggttt cgtcaagtac ctcgccgatc accgggttgt cgatcaggct
     5041 gtcggtgtcg atctgctcgt cgtaaatggt gccgaacagc aggttctcgg cgaccgtcat
     5101 gttcaggttg tacttgcggt gatcgaaagg ctcgaccagc ttttcgacgg cggaatcggt
     5161 gaaacggtcg cgtaactggc ggcgggcttc catgatacgc gccgcgaaat ccgcatgttt
     5221 cgactggtcg acgaaggatt gcagcccgaa ctggtagatt tcctcgtcga gttcgacgca
     5281 tttgagggtc tcgacgatgc gctcgatgag ctgctcccga tccgcgcaat cgagggcctg
     5341 gtaatcgatc cactcatcgt cgtaacgatc cggcgtatta cccgccaggg ccgcgagatg
     5401 gcgttcctgt tcgagtgcct tgtccggctt ttggtcggcg gatttgaccg gctgatgttt
     5461 cagcgagtag gtcagattgt cgaataccga gccgttgaac atgaatggcg acgagccgac
     5521 gtagccgagc cggttgccgg tgatcgcctc gggcaattcc tgcatttttg cgttgccgag
     5581 gatcaggttg ccggagctgg ggtgcagcag tcgcgagacc aggccggcga gttcgtcctt
     5641 gccgctgttg ccgaggccaa ccgcggcgca gtgctgatcc acgggcagtt tgaagctggc
     5701 accgtcgatg ctgaaataga ggccatcctc gctgtaacgc aggttgttgg cacggatttc
     5761 ctggcctggc aaggccaccg attcggggcg ctcattgatg atttcgggtt cgatcaggtt
     5821 gtgcgggtcg aattgctgaa tgacctgggt gtacttgacc ttgacatctt ccatgcgctg
     5881 gtagtaacgc agcaactctt tccacggtcc cgacaggtct ttgtaggcga ccagcacggc
     5941 aaccatcgag ccgatcgaca ggtcgccgcg caatacgaaa tagccgccca cagaataaaa
     6001 gaaaaacggc gtcagctggg cgataaaatt gttgagaaac ttgatgaaaa acttgcgctt
     6061 gtaaatgtcg aagcgaatca gatagatgcg accgagtcgc tggctgatat gggcacgctc
     6121 gaagcggctg gtatcgttgg cgtgcacgtc gccgataccg gaaatggttt cgccgatgcg
     6181 tcccgacagg ttgcgcaccg tggacacgcg ctccttgctg agttcgttga ctcgcctttg
     6241 cagtctcgga atcacgtaga gctggaacgg gtagagcgcg atcgccgcca ggccgaggta
     6301 aagatcctgc tgaaatatga agaacaggta ggtcagcagg gtgccgccct ggaataccgg
     6361 cagggtgaag gattcaccga taaattcgcc cagcggctcg gtttcggcgg tgatcatcgg
     6421 gatcagttcg cccgatgaga cgcgtttgaa gtgcggcagc ggaaagcgca ggatgcgttc
     6481 gtagagctcg tagcgaaagc gtcgcaggat gcgctcgccg agcgcgccgc ggtaaacgtt
     6541 gagcaggtac ttgagaccgc cgctcgacag caccaccagc aagaatgcga aactcagcgc
     6601 catcaggaat tcgaggcggt cgagttcgaa gccgagcagt tggtcgggca actctacgcc
     6661 ctcgagcagg ttaatgattt tcttgggtaa ttcgagggtg atgtaaacca gcggtaacga
     6721 cgccagagac agcagcacca gcagcagttg gtctcgtttg ctgtgggtta gaatgtacct
     6781 gtatagcgac ggttccataa actcccggat attgaatcta gttctggttc cttggcccgg
     6841 gtcccgcgac tggcgtcggt agcacggcaa gtcgggccag tgtactatcg ccgatcattc
     6901 gatttatatc aaataccgcc tatgccacct caggtagccg tcgttgtgaa gggttatccg
     6961 cgcctctcgg aaacctttgt cgcccaggaa atccgggcgc tggaactacg cggcttcgaa
     7021 ttgtgcattt tttcactgcg ccatccctat gacccggcga tccacccggt gcatgcggaa
     7081 atcgaggcgc cggtgtacta cctgcccgaa tatgcgcacg atgactggcc gcgcgtgctg
     7141 tcggcctgcc tgcggctgtg cctgcgcgcg ggtttctggc gggcgctggt ccggctcgcc
     7201 gccgacctgc tgcacgaccc gacccgcaat cggctgcggc gtttcgcgca ggccgcggtg
     7261 atgagcgtcg agatgccggc gacggtgcgc cattattacg tccatttcat gcataccccg
     7321 gcatcggtcg gttactactc tagcctgatc agcggtcgca gttggagcat ttcggcgcat
     7381 gccaaggata tctggacgct cgaccggcgc gaactcgccg ccaagctcgc cagcgctgac
     7441 tggacggtca cctgcactgc cgccaaccgg cgctacctgg cgcaaatcgc tgatcgtgac
     7501 gacaaggttg cgctgctcta ccacggactc gatttcagcc gattcgaact caaccccggc
     7561 catccggcac aacgcgacgg gcgcgatccg acgcacccgg tcaggctgat cagcgtcggc
     7621 cgcgcggtgg acaagaaagg ctacgacgac ctgctgcggg cgctggcgga actgcccgcc
     7681 gacctgcact ggcggttcac
//
LOCUS       DATCZV010000186         3795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_649717, whole genome shotgun sequence.
ACCESSION   DATCZV010000186 DATCZV010000000
VERSION     DATCZV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649717"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VIW27_04165"
     CDS             <1..276
                     /locus_tag="VIW27_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5738892.1"
                     /translation="ISNPLDIPVGPILSMKELAEEPALRETGTIVECDHPERGPYLSV
                     GCPVKLSATDVTVERSPLLGEHSEEILGEVLGYNAAEIAALKEAGAV"
     gene            325..1239
                     /locus_tag="VIW27_04170"
     CDS             325..1239
                     /locus_tag="VIW27_04170"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5738893.1"
                     /translation="MKLIVHGQQAYGKSVLEAILDKGVDEVVAVYCAPDKEGRPLDPL
                     KEFALEKGLPVHQPASWKDPEVWNQLASHNADLCVMAYVLLFVPEEALNVPKYGSIQY
                     HPSLLPWHKGPSSINWPIIMGKEKTGLSIFWPDNGLDTGPILLQKEVDIGPDDTLGSI
                     YFDKLFPLGVEAMMEAIELVRDGKAPKQVQNPDDGSYEGWCRKQDVEIDWSKSTDEVY
                     NLIRGANPQPGAWTTNGGAELKIFDSRKAEGSGDPGTVLAIGDDGVVIATADGAIRAI
                     KVRADAGKVPAQEYAAEAGLAEGSKLGG"
     gene            1290..2957
                     /locus_tag="VIW27_04175"
     CDS             1290..2957
                     /locus_tag="VIW27_04175"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY5738894.1"
                     /translation="MSDIEIARAATAQPITEIAAKLGIPAEAILPYGHTKAKVTPEYI
                     ESLEDRPDGKLILVTAISPTPAGEGKTTTSVGLNDGLNRIGKKSVVCLREPSLGPCFG
                     MKGGAAGGGYAQVIPMEDINLHFTGDFHAIGAAHNLLSAMVDNHIYWGNEAGLDARRI
                     AWKRVVDMNDRALRSIVNSLGGPGNGFPREDGFDITVASEVMAIFCLSKNLADLRERL
                     NRIIVGYTREREPVTAEQMNAPGAMAVLLKDAIAPNLVQTLENNPAFIHGGPFANIAH
                     GCNSVIATKTALKLADYVVTEAGFGADLGAEKFFDIKCRSAGLKPDAAVIVATIRALK
                     MHGGVALADLGSENVDAVAKGCSNLARHIRNVKSFGVPVVVAINRFSADTDAEVAKVR
                     EVTREYGVNAIDCTHWANGGAGTEELANQMVDIIAKDSSQFSPLYGSDMPLWEKMRTI
                     ATHIYGANDIIADKKVREQIDQFQQSGFGNLPICVAKTQYSFSTDPNLKGAPSDHVVP
                     IREVRLSAGAGFLVFVCGEIMTMPGLPRVPAADKIHIDEAGLIQGLF"
     gene            2972..>3795
                     /locus_tag="VIW27_04180"
     CDS             2972..>3795
                     /locus_tag="VIW27_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5738895.1"
                     /translation="MIKIILVPLDGSERSAAVLDTALIVAGRFDAHIKVVHVRERVSE
                     PFLFAEMPDSLREQVMKMNSQASESVVDTVREQFHQFCKRGKVKVTRKPSSAKQVSAS
                     LHILEGDTVSVLDRESRLVDVIAMSRPVQHRLGGRAVGELHESLMMHSGRPVLVVPPD
                     WTPHRVDHAAIGWNDSVEASRALAITLPWLAQMKKVTVIVSRKREAGVGDVVEYLKHH
                     GCKADYHVISSGGNVGKKMLAACKEIDAEFLVVGGFSHTRTRQRFFGGVTSHLLANT"
BASE COUNT          758 a         1169 c         1227 g          641 t
ORIGIN      
        1 atcagcaacc cgctggatat tccggtcggg ccgatcctgt cgatgaagga actggccgaa
       61 gaaccggcgc tgcgcgagac cggcaccatc gtcgagtgcg atcaccccga gcgtgggccc
      121 tacctgtcgg tgggctgtcc ggtcaagctt tcggccaccg atgtgacggt cgagcgctcg
      181 ccgctgctcg gcgagcattc cgaggaaatt ctcggcgagg tgctcggtta caacgcggcg
      241 gaaatcgcgg cgctcaagga agcgggtgcg gtttaagaca atcatggcgc gcgctgatgg
      301 cgcgcgcggc ggcaggaggt gaagatgaaa ttaatcgtac atggccagca ggcctacggc
      361 aaatcggtcc tcgaggcgat cctcgacaag ggcgtcgacg aggtggttgc ggtttactgc
      421 gcgcccgaca aggaaggccg gccgctggac ccgctgaaag aattcgccct cgaaaagggt
      481 ctgccggtgc accagccggc atcctggaag gatcccgagg tctggaatca gctcgccagc
      541 cacaacgccg atttgtgcgt gatggcctat gtgttgctgt tcgtacccga ggaggcgctc
      601 aacgtgccga agtacggctc gattcaatac cacccgtcgc tgttaccgtg gcacaaggga
      661 ccgagttcga tcaactggcc gatcatcatg ggcaaggaga agaccggcct gtcgatcttc
      721 tggcccgaca acggcctcga taccggcccc atcctgctgc agaaagaagt cgacatcggt
      781 cccgacgaca ccctcggttc gatctacttc gacaagctgt tcccgcttgg tgtcgaggcg
      841 atgatggaag ccatcgaact ggtgcgcgac ggcaaagcgc cgaaacaggt gcagaatccg
      901 gacgatggca gctacgaggg ctggtgccgc aagcaggacg tggaaatcga ctggagcaag
      961 tccaccgacg aagtctacaa cctgatacgc ggcgccaatc cgcagcccgg cgcctggact
     1021 accaacggtg gcgccgagct gaagattttc gacagccgca aggccgaggg cagcggcgat
     1081 ccgggcaccg tgctcgcgat tggtgacgac ggcgtcgtaa tcgcaacagc ggatggcgcg
     1141 attcgcgcga tcaaggtacg ggccgatgct ggtaaagtgc ctgcccagga gtatgctgcc
     1201 gaagccgggc tcgcggaagg ctcgaagctc ggcggttgat cgcgacgcaa acaacgacag
     1261 tgccggcgcg ccggccaagg tgagacagaa tgagtgatat cgaaatagcc cgcgcggcta
     1321 ccgcgcaacc gattaccgag atcgccgcaa aactgggaat acccgccgag gcgatcctgc
     1381 cttacggtca caccaaggcc aaggtcacac cggagtatat cgagtcgctc gaggatcgcc
     1441 ccgacggcaa gctgatcctg gtgaccgcga tctcgccgac gccggcgggc gagggcaaga
     1501 ccacgacatc ggtcgggctc aacgacggcc tcaaccgcat cggcaagaag tcggtggtct
     1561 gtctgcgcga gccgagtctc ggaccctgct tcggcatgaa gggcggcgcc gccggcggcg
     1621 gctacgcgca ggtgattccg atggaagaca tcaacctgca cttcaccggc gattttcatg
     1681 ccatcggcgc ggcccacaac ctgctgtcgg cgatggtcga caaccatatc tactggggca
     1741 acgaggccgg cctcgatgcg cgccgtatcg cctggaagcg cgtcgtcgac atgaacgacc
     1801 gcgcgctgcg ctcgatcgtc aactcgctcg gcgggccggg taatggtttt ccgcgcgagg
     1861 acggtttcga tatcacggtg gcctccgagg tgatggcgat tttctgcctg tcgaagaatc
     1921 tggccgattt gcgcgagcgc ctgaaccgca tcatcgtcgg ctacacgcgc gagcgcgagc
     1981 cggtcaccgc cgagcagatg aacgcgcccg gcgcgatggc ggtgctgctg aaggacgcga
     2041 tcgcgcccaa cctggtgcag acgctggaaa acaacccggc cttcatacac ggtggcccgt
     2101 tcgccaatat cgcccacggc tgcaactcgg taatcgccac caagaccgcg ttgaagctgg
     2161 ccgactacgt ggtcacggag gccggctttg gtgccgatct cggcgcggag aaatttttcg
     2221 acatcaagtg ccgcagcgca ggtctcaagc ccgatgcggc cgtcatcgtc gccaccattc
     2281 gcgcgctgaa gatgcacggc ggcgtcgcgc tcgccgatct cggcagcgaa aacgtcgacg
     2341 ccgtcgccaa gggctgcagc aacctcgcgc ggcacattcg caacgtcaag tccttcggcg
     2401 tgccggtagt ggtcgcgatc aaccgcttca gcgccgatac cgatgccgag gtcgcgaaag
     2461 tgcgcgaggt gacgcgcgaa tacggcgtca acgccatcga ctgcacgcac tgggccaacg
     2521 gcggtgccgg taccgaggaa ctcgccaatc aaatggtcga tatcatcgcc aaggattcga
     2581 gccagttttc gccgctctac ggcagcgata tgccgctgtg ggaaaaaatg cgcaccatcg
     2641 cgacccatat ctacggtgcc aacgatatca tcgccgacaa gaaagtccgc gagcagatcg
     2701 accagttcca gcagagtggc ttcggcaacc tgccgatctg tgtcgccaag acgcagtaca
     2761 gtttctccac cgatccgaat ctcaagggtg cgcccagcga ccacgtggtg ccgattcgcg
     2821 aggtgcggct gtctgccggc gccggtttcc tggtgtttgt ctgcggtgaa atcatgacca
     2881 tgccgggcct gccccgggtg ccggcggccg acaagattca tatcgacgag gcgggcctga
     2941 tccagggcct gttctgagtg agggcaagct gatgatcaag ataattctgg tacccctgga
     3001 cggctcggag cgcagcgccg cggtgctcga taccgcgttg atcgtcgccg ggcgtttcga
     3061 cgcgcacatc aaggtggtgc acgtgcgcga gcgggtcagc gagccgttcc tgttcgcgga
     3121 aatgcccgac tcgctgcgtg agcaggtcat gaaaatgaac agccaggcct cggaatcggt
     3181 ggtcgatacg gtgcgcgagc aatttcacca attctgcaag cgcggcaagg tcaaggttac
     3241 ccgcaagccg tcgagcgcca agcaggtatc ggcctcgctg cacattctcg agggtgatac
     3301 cgtcagcgtg ctcgatcgcg agtcccggct ggtggacgtg atcgcgatgt cacgcccggt
     3361 acagcatcgg ctcggtggcc gcgcggtcgg cgaattgcac gaatcgctga tgatgcattc
     3421 cggccgcccg gtgttggtag tgccgccgga ctggacgccg caccgcgttg accacgcggc
     3481 gattggctgg aacgacagcg tcgaggccag ccgtgcgctg gcgattacgc tgccgtggct
     3541 cgcacagatg aagaaggtga cggttatcgt ctccaggaaa cgcgaggccg gtgtcggcga
     3601 cgtcgtcgag tatctcaagc accacggctg taaagccgat tatcacgtga tcagcagcgg
     3661 cggcaacgtc ggcaagaaga tgctggctgc ctgcaaggaa atcgacgccg agttcctggt
     3721 ggtcggcggc ttcagtcaca cgcgcacccg ccagcgcttt ttcggcggcg ttaccagcca
     3781 cctgctcgcc aacac
//
LOCUS       DATCZV010000187         8436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_226647, whole genome shotgun sequence.
ACCESSION   DATCZV010000187 DATCZV010000000
VERSION     DATCZV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226647"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VIW27_04185"
     CDS             <1..589
                     /locus_tag="VIW27_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5738896.1"
                     /translation="EYGHILTVEDPIEFVHESKKCLVNQREVHRDTLGFNEALRSALR
                     EDPDTILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKSMV
                     RSMLSESLKAVISQTLMKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGQN
                     VGMQTLDQNLKTLLAQGVVAKDEAMRKAAHPDALA"
     gene            616..1785
                     /locus_tag="VIW27_04190"
     CDS             616..1785
                     /locus_tag="VIW27_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5738897.1"
                     /translation="MDRNKAISFMHDLLRMMVSKNGSDLFITTGSPPAMKIDGKVAAV
                     SKQNLSPNHTQMLARSIMNDKQLSEFEEKQECNFSIALPGVARFRVNAFVQRGSSGMV
                     LRIINSEIPSFDELNLPPILKDIAMTKRGLVIFVGGTGSGKSTSLASMVDYRNRNSYG
                     HIITIEDPVEFVHNHQNCLVTQREVGVDTESYEIALKNTLRQAPDVILIGEIRDRETM
                     EHAIAFAETGHLCLSTLHANSTNQALDRIINFFPDERRQQLLMDLSLNLKGMVSQRLI
                     PKLNSKGRIPAIEVMLNTPLMADLIFKGDVHEMKSLIAKSNEAGMQTFDQALFNLFEA
                     EKITFEDALRNADSVNDIRLRIKLESDVARKTGMVEDHRDFELEDSDDDAAGGMI"
     gene            1803..2972
                     /locus_tag="VIW27_04195"
     CDS             1803..2972
                     /locus_tag="VIW27_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019594499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5738898.1"
                     /translation="MSQEPTTKLLEPYLKVMVEKQASDLYFVTGAAPNIKIQGSTAAI
                     AKNPFQAGQVQKLAYSLLTDEQIRDFEINRELNLGFTLADVGRFRVNIFIQRSEVSMV
                     IRYIKWDIPTIEELGLPAVLKQIVMNQSGLVLVVGSTGSGKSTTLASMLNHRNETAGG
                     HILTIEDPIEFVFRHVRSIVSQREVGIDTLTYGNALREALREAPEVIMIGEARDSETM
                     KAAINFADTGHLCLTTLHAVNSNQAMDRIMNMFSTEMRPQLLMDLSLNLKAVISQRLV
                     PGMQGRLACAVEIMMNTPYISELLRDGNFNEIKAIMEKGDTTGMQTFDQSLFDLYKAD
                     RISIKDALAYADSSTNLEWKINFGGDKASSQDTRHNLNQQGDLEELTLPSEEGQD"
     gene            complement(2969..4252)
                     /locus_tag="VIW27_04200"
     CDS             complement(2969..4252)
                     /locus_tag="VIW27_04200"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5738899.1"
                     /translation="MKLQIRNGLLVEPGEAKTRKADVFVENGRIAAIGKAPTGFKAQQ
                     TIDAAGHWILPGLIDCQARLRDPGQPEKANIASETAAAVRGGITTLCLPPDTQPPIDN
                     SATVELIHHRNEMFGHKARIYPIGALTRGLGGEQLSNMASLRDNGCIAFGNALFPLAS
                     NLVQRRAMDYAAGLRSLVVIQPLDIELAANGCAHEGAIATRLGLPAIPEAAETAALAK
                     DIELVAQTGARTHFGQLSCARSVEMIGRAKAKGLPVSADCAIHQLFLTDHDIGMFNSN
                     MLVIPPLRSQYDRDALRAGVADGIIDCLCSDHQPHEVDAKLKPFPSAEPGISGLDTLL
                     ALALRLVDEELLPLGELIARLTANPAQIFDLPGGRIAAGEIADLIIVDPETHWICKAE
                     NFVSKGRNTAFEGWDFRGSVTHTLIDGEIVYQAAG"
     gene            complement(4249..5226)
                     /locus_tag="VIW27_04205"
     CDS             complement(4249..5226)
                     /locus_tag="VIW27_04205"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY5738900.1"
                     /translation="MTDNIQLDDNGKLRHFLGIEGLNQTIITEILDTAASFTSVTDRT
                     IKKVPILRGKTVANLFFEASTRTRSTFELAEQRLSADILSLNVDTSATVKGESLLDTL
                     QNLEAMQIDMFVIRHQDSGSAHFFAQHVRPGVSVLNAGDGQHEHPTQAMLDMYTIRHH
                     KQDFTRLRVAIVGDILHSRVARSQIHALNLLNTAEIRVIAPRTLLPVETESLGVKVYE
                     DLERGLEDVDVVIMLRLQKERMRGALLPSEKEYFRKYGLSQERLRRARPDAIVMHPGP
                     ANRGVEIESSVMDGGQSVILQQVSFGISVRMAVMSIVVGQSGLKSGGAT"
     gene            complement(5219..5737)
                     /gene="pyrR"
                     /locus_tag="VIW27_04210"
     CDS             complement(5219..5737)
                     /gene="pyrR"
                     /locus_tag="VIW27_04210"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY5738901.1"
                     /translation="MTLPAVESLLDKLSQALTPYFSSTESQPVIVGIETGGAWVAERL
                     RQRIAPDAELGRLNISFYRDDFTRSGLHPTVKPSSLPTDIDERTIVLVDDVLHSGRTV
                     RAAMNEIFDYGRPKRIVLAILIDRGEREIPIQADVVGATMKLDAGSHIKLEGPEPLRL
                     VVKTLEPEDPHD"
     gene            6141..6485
                     /locus_tag="VIW27_04215"
     CDS             6141..6485
                     /locus_tag="VIW27_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY5738902.1"
                     /translation="MELPLQITFRDLPRSEALEADIRDKAGKLDQFYEKVMACRVVVE
                     RPHGHQYKGELFHIRIDLTVPGRELVVNRGPDQHHAHEDPYVAVRDAFDAARRRLQDF
                     ARKQRGDVKHHQ"
     gene            complement(6823..7080)
                     /locus_tag="VIW27_04220"
     CDS             complement(6823..7080)
                     /locus_tag="VIW27_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5738903.1"
                     /translation="MAARLRQKLKARCLLAFLSLLRFAEVSTKVELPTVNDMAAILGV
                     TAESVRRILAEFKRQRILQPMDYSPREGDRIVFPRLRQQTC"
     gene            complement(7053..7466)
                     /gene="ruvX"
                     /locus_tag="VIW27_04225"
     CDS             complement(7053..7466)
                     /gene="ruvX"
                     /locus_tag="VIW27_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012323974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY5738904.1"
                     /translation="MNESLTTVIGFDYGSKWIGIAVGQTLTRQARPLGAIKTGDWNAI
                     KQVIDDWRPQRLIVGLPMNMQGEQQEMSARAERFGRQLEGRFGITTSCIDERLTTREA
                     YTVAIDSGERKTKQQIDSLAAALITESWLRDYDKN"
     gene            complement(7477..8043)
                     /locus_tag="VIW27_04230"
     CDS             complement(7477..8043)
                     /locus_tag="VIW27_04230"
                     /inference="COORDINATES: protein motif:HMM:NF001266.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5738905.1"
                     /translation="MNQQTASLVDKCLIATPAIKDPIFASSLVYMCEHNQDGSMGLVV
                     NHETSQVLDDIFQQLNIDCDDERIRNQPVYIGGPVQLEQGFVLHAGGGHWQNSIEVSS
                     GIHLTSSLDILQAIASGQGPEDYLVILGFSGWASGQLESELQQNSWLTSACNADLLFH
                     EKPEDKWQVAFDSLGFDINSLSPVSGNA"
     gene            complement(8053..>8436)
                     /locus_tag="VIW27_04235"
     CDS             complement(8053..>8436)
                     /locus_tag="VIW27_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY5738906.1"
                     /translation="IDYAKRPRTLTLTASTRKAVEASYLASWVQKIERTGNLNYPREA
                     RSRNIDGRLRMSVRLNAAGEVLNMEITSSSGTSVLDEAAKRILRMAQPFAPFSDELRE
                     RADQIVIIRTWDFKSNRFQTQSGVS"
BASE COUNT         1657 a         2500 c         2613 g         1666 t
ORIGIN      
        1 cgagtacggg catatcctga ccgtcgagga tccgatcgaa ttcgtgcacg agagtaagaa
       61 gtgcctggtc aaccaacgcg aggtgcatcg cgatacgctg ggattcaacg aagccctgcg
      121 ttcggcgctg cgcgaagacc cggacaccat cctggtcggc gagatgcgcg atctggaaac
      181 cattcgcctg gcattgtcgg ccgccgaaac cggtcacctg gtgttcggca cgctgcatac
      241 cagctcggcg gcgaagacca tcgaccgtat cgtcgacgtg ttcccggccg ccgagaagtc
      301 gatggtcagg tcgatgctgt cggagtccct gaaggcggtc atctcgcaga ccctgatgaa
      361 aaaaatcggt ggcggccgcg ttgccgcgca tgagatcatg atcggcacgc ccgcgattcg
      421 caacctgatt cgcgaggaca aggtggcgca aatgtactcg gcgattcaga ccgggcagaa
      481 cgtcggcatg cagacgctgg accagaatct gaagacattg ctcgcccagg gtgtggttgc
      541 caaggacgag gccatgcgca aagcggcgca tcccgatgca ctggcgtaag cagctgaaat
      601 tatcggggga atacaatgga tcgtaacaag gccatcagct tcatgcacga cctgttgcgc
      661 atgatggtga gcaagaacgg atcggacctg ttcatcacca cgggttcgcc accggcgatg
      721 aaaatcgacg gcaaggtcgc cgccgtatcc aagcaaaacc tgtcgccgaa tcatacccag
      781 atgctggcgc gctcgatcat gaatgacaag caattgtcgg aattcgagga gaaacaggaa
      841 tgtaattttt cgatcgcgct gcccggtgtc gcccgcttcc gtgtcaacgc tttcgtgcaa
      901 cgcggcagct ccggcatggt gctgcgtatc atcaactcgg aaatcccgag tttcgatgaa
      961 ctcaacctgc cgccgatact gaaagacatc gcgatgacca agcgcggcct ggtgattttc
     1021 gtcggcggta ccggctccgg taaatcgact tcgctggcgt cgatggtcga ttaccgcaac
     1081 cgcaattcct acggccatat catcaccatc gaggacccgg tggaattcgt tcacaatcac
     1141 cagaactgcc tggtcaccca gcgcgaggtt ggcgtcgata ccgagtctta cgaaattgcc
     1201 ctgaaaaaca cgctgcgcca ggcgcccgac gtgattctga tcggcgagat ccgcgaccgc
     1261 gaaaccatgg agcacgcgat cgcgtttgcc gaaaccggtc atctgtgcct gtcgacactg
     1321 cacgccaaca gcaccaacca ggcactcgac cgtatcatca acttctttcc tgacgagcgc
     1381 cgccagcaat tgctgatgga cctgtcgctg aacctgaagg gcatggtgtc gcagcgcctg
     1441 attccgaaac tgaacagcaa gggccgcatc ccggcgatcg aggtcatgct caacacgccg
     1501 ttgatggcgg acctgatctt caagggcgac gtgcatgaga tgaagtcgct gatagccaag
     1561 tccaacgaag ccggcatgca aaccttcgac caggccttgt tcaatctttt cgaggcggag
     1621 aagattactt tcgaagacgc gttgcgcaac gccgactcgg tcaacgatat tcgcctgcgc
     1681 atcaagctcg aaagcgatgt cgcgcgcaag acgggtatgg tcgaggatca tcgtgatttc
     1741 gagctcgagg attccgatga tgacgccgcg ggcggcatga tctaggcgag aagagagacg
     1801 cgatgtcaca agaaccgacg accaagctgc tcgagcccta cctgaaggtc atggtcgaaa
     1861 agcaggcttc cgacctgtat ttcgttaccg gcgcggcgcc gaacataaaa atccagggca
     1921 gtaccgcggc aatcgccaag aacccgttcc aggccggcca ggtgcaaaag ctggcctata
     1981 gcctgctgac cgacgagcag atccgcgatt tcgaaatcaa ccgcgaactg aacctcggct
     2041 tcacgcttgc cgatgtcggc cggtttcgcg tcaatatctt cattcaacgt tccgaagtgt
     2101 cgatggtcat tcgctacatc aagtgggata tcccgaccat cgaagaactc ggcttgccgg
     2161 cggtgctcaa gcaaatcgtc atgaaccagt cggggctggt gctggtcgtg ggatccaccg
     2221 gctccggtaa atcgaccacc ctggcgtcga tgctgaatca tcgcaatgaa accgccggcg
     2281 gccatatcct gacgatcgag gacccgatcg agttcgtatt tcgccacgtc aggtccatcg
     2341 tatcgcagcg tgaagtcggc atcgacacgc tgacttatgg caacgcgctg cgcgaagccc
     2401 tgcgcgaggc gccagaggtg atcatgatcg gcgaggcgcg tgattccgag accatgaaag
     2461 cggcgatcaa tttcgccgat accggccacc tgtgcctgac cacgctgcac gcggtcaact
     2521 cgaaccaggc gatggaccgc atcatgaaca tgttttcgac cgaaatgcgt ccccagttgc
     2581 tgatggacct gtcgctgaat ctgaaggccg ttatctctca gcgcctggtg ccgggtatgc
     2641 agggcaggct cgcctgcgcc gtggaaatca tgatgaatac cccctacatc tcggaattgt
     2701 tgcgcgatgg taacttcaac gagatcaagg caatcatgga aaagggcgat accaccggca
     2761 tgcagacctt cgaccagtct ctcttcgatc tttacaaggc ggatcggatt tccatcaagg
     2821 acgcgctcgc ctacgccgat tcgagcacca acctggaatg gaaaatcaac ttcggcggcg
     2881 acaaggcctc gtcacaggat acccgtcata acttgaacca gcagggcgat ctcgaggaac
     2941 tgacgctgcc ttccgaggaa ggccaggact aacccgccgc ctggtaaacg atctcgccgt
     3001 cgatcagcgt gtgggtcacg ctgccgcgaa aatcccagcc ttcgaaggcc gtgttcctgc
     3061 ccttggaaac gaaattctca gctttgcaga tccagtgggt ttcgggatcg acgatgatca
     3121 ggtcggcgat ttcaccggcc gcgatgcgac cgccgggcag atcgaagatc tgcgccgggt
     3181 tggcggtcag ccgcgcgatc agttccccca gcggcagcag ctcctcgtcg accagccgaa
     3241 gcgccagtgc cagcagcgta tcgagcccgc tgatgccggg ttcggccgaa ggaaaaggtt
     3301 tcagcttggc gtccacttca tgcggctggt ggtccgaaca caggcagtcg atgatgccat
     3361 cggcgacgcc ggcacgcagt gcatcgcgat cgtactggct gcgcagcggc ggaatgacca
     3421 gcatgttgct gttgaacatg ccgatgtcgt gatcggtcag gaacagctgg tggattgcgc
     3481 aatcggcgct taccggcaga cccttggcct tggcgcgacc gatcatctcc accgagcgcg
     3541 cgcaggacag ctggccgaaa tgagtgcgcg caccggtttg cgcgaccagt tcgatatcct
     3601 ttgccagcgc cgcggtttcg gcagcctcgg gaatcgccgg cagcccgagg cgggtagcga
     3661 tcgcgccctc gtgggcacag ccgttggccg ccagttcaat gtccagcggc tggatcacca
     3721 ccagcgaacg caggcccgcc gcgtagtcca tcgcgcggcg ttgcaccagg ttgctggcga
     3781 gcggaaacag cgcgttgccg aaggcgatac agccgttgtc cctgaggctc gccatgttgc
     3841 tcagctgttc gccgccgaga ccgcgtgtca gtgcgccgat cgggtaaatc cgtgccttgt
     3901 ggccgaacat ttcgttgcgg tgatggatca gttcgacggt cgccgagttg tcgatcggcg
     3961 gctgcgtatc cggcggtaag cacagcgtgg tgatgccgcc cctgaccgcg gccgcggttt
     4021 cgctggcgat attggctttc tcgggttgcc cgggatcgcg cagacgcgcc tggcaatcga
     4081 tcagccccgg caggatccag tgcccggcgg cgtcgatggt ctgctgcgcc ttgaagccgg
     4141 tcggcgcctt gccgatcgcc gcgatgcggc cgttctcgac gaaaacgtcg gccttgcgcg
     4201 tcttcgcttc cccgggttcg accagcaggc cattgcgaat ttgcagtttc acgtcgcgcc
     4261 cccggacttg aggccggact ggccgaccac gatcgacatc accgccatgc gcaccgagat
     4321 gccgaagctg acttgctgca ggatcaccga ctggccgcca tccatgacgc tggactcgat
     4381 ctcgacgccg cggttggcgg gtcccgggtg catgacgatg gcatcgggcc tggcgcgccg
     4441 cagccgctcc tgcgacaggc cgtacttgcg aaagtattcc ttttcgctcg gcagcagcgc
     4501 gccgcgcatg cgctcctttt gcaggcgcag catgatcacc acgtcgacgt cctcgagccc
     4561 gcgctcgaga tcctcgtaga ccttgacgcc gagcgactcg gtttcgaccg gcagcagggt
     4621 gcgcggcgcg atgacgcgaa tctcggcggt gttcagcagg ttaagggcgt gaatctgcga
     4681 gcgcgccacc cgcgagtgca ggatatcgcc gacgatggcg acgcgcaggc gggtgaaatc
     4741 ctgcttgtgg tgacgaatcg tgtacatgtc gagcatcgcc tgggtggggt gctcgtgctg
     4801 gccgtcgccg gcgttgagca ccgatacccc ggggcgcacg tgctgggcga aaaagtgcgc
     4861 gctgccgctg tcctggtggc ggatcacgaa catgtcgatc tgcatcgcct cgaggttttg
     4921 cagcgtatcg agcagcgatt cgcccttgac cgtagccgag gtatcgacgt tgaggctcag
     4981 aatatccgcc gacaggcgct gctcggccag ctcgaaggtc gagcgggtgc gcgtgctggc
     5041 ttcgaaaaac aggttggcga cggtcttgcc gcgcaggatc ggcacctttt tgatggtgcg
     5101 gtcggtgacc gaggtgaagg atgccgcggt gtcgagaatt tcggtgatga tcgtctggtt
     5161 cagcccctcg atgccgagga agtgtctgag cttgccatta tcgtcgagct ggatattgtc
     5221 agtcatgcgg atcttcgggc tccagcgtct tgacgaccag gcgcagcggt tcgggtccct
     5281 cgagcttgat gtgactgccg gcgtcgagtt tcatggtggc gccgaccacg tccgcctgga
     5341 tcgggatctc gcgctcgccg cgatcgatca ggatggcgag cacgatgcgt ttcggccgcc
     5401 cgtaatcgaa gatttcattc atcgccgcgc gcaccgtgcg tcccgagtgc agcacgtcgt
     5461 cgaccagcac gatggtgcgc tcgtcgatat cggtcggcag gctcgacggc ttgaccgtcg
     5521 gatgcaggcc gctgcgggtg aagtcgtcgc ggtaaaagga aatgttcaat cgcccaagtt
     5581 cggcatcggg agcgattctt tgccgcaggc gctcggcgac ccacgcgccg ccggtctcga
     5641 tgccgacgat gacgggttgc gattcggtag aggaaaaata gggagtcagt gcctggctta
     5701 gcttatcgag cagcgattcg acggcgggta aggtcactta ggctccatgg atacgtgcgc
     5761 tggctttata gcataaatcg agcttgaaaa cagcagactt gacccggacc aatggctcga
     5821 taccgagctc gggcaaacgg gtatccgtgg cccggccgct gtgtagttcg gggtatgcct
     5881 cgtcattgca ttctgcccgc gcatgcagcg atttcgagcg gcaggctgtc gatcggcggc
     5941 gcggtcattt cgaacaccct cgtcggcagc atggaacgac ccgcgatagt ctgacgatca
     6001 tcgatagtaa tgcctccatt tcactggcct ttgatgtaag tcaggatctc gcgatccgct
     6061 ccgggagatg ctaattaccg taaggaaaat ctgcgctatg agcggatttt cgagatttac
     6121 cagcaaccga aaggtgacta atggaactgc cactgcaaat cacgtttcgc gatctaccgc
     6181 gctccgaggc gctggaagcc gacatccggg acaaggccgg gaaactcgac cagttctacg
     6241 aaaaagtcat ggcttgccgc gtggtcgtgg aaagaccaca tggccaccag tacaagggag
     6301 agctgtttca tatccgcatc gacctgaccg tgcccggcag ggaactggtg gtcaatcgcg
     6361 ggcctgatca gcaccatgct cacgaagacc cttacgtcgc ggtgcgcgac gccttcgatg
     6421 ctgcacgccg ccggctgcag gacttcgcgc gcaagcagcg cggcgacgtc aagcaccacc
     6481 agtagccgcc gcacggcaag atactcgagg tgccaccttt gcaggactaa ggcacggtcg
     6541 aaagtgccgg cggccggtaa atctgttttc atcgccacag ggtcgccgac ggcgatttcg
     6601 aagcaatcgt ggttggcgac gagaccgacc aggtttgtcg aggaagccgc cgtcgagaga
     6661 acccgggaga gtcgcgtgca agcggtcggc aagccccatc gcagcgaaca ggattttccg
     6721 cagaggcggt gaattttcgg gttgccggct ggtaccggaa gacgtcttaa tgcctgtaaa
     6781 cgaagccata cgcggcgggg cgtccgtgtg tcctgagaat cgttagcagg tttgctgcct
     6841 tagtcgcgga aaaacgatcc ggtctccttc tcgcggcgaa tagtccatcg gttgcaggat
     6901 ccgctggcgc ttgaattcgg ccaggatgcg gcgcacgctt tccgcggtta caccgaggat
     6961 agccgccatg tcgtttacgg tcggcagttc gacctttgtc gacacttcgg cgaacctgag
     7021 cagcgacaga aacgcgagca ggcagcgggc cttcagtttt tgtcgtagtc tcgcagccag
     7081 ctctcggtga tcagcgcggc ggcgaggctg tcgatttgct gcttcgtctt gcgctctccg
     7141 ctatcgatcg cgacggtata ggcttcccgg gtcgtcaggc gctcgtcgat acaactggtg
     7201 gtgataccga agcggccttc gagttgacgc ccgaaacgct cggcgcgcgc gctcatttcc
     7261 tgctgctcgc cctgcatgtt catcggcaga ccgacgatca gtcgctgtgg ccgccagtcg
     7321 tcgattacct gtttgatggc gttccagtcg ccggtcttga tcgcgccgag cgggcgcgcc
     7381 tggcgggtca gggtctggcc gacggcgata ccgatccatt tgctgccgta atcgaagccg
     7441 atcacggtag tcagcgattc attcatcgac gtgaattcag gcgttgccgc tgaccgggga
     7501 gagcgaattg atatcgaagc cgagcgaatc gaaagccacc tgccacttgt cctcgggctt
     7561 ttcgtgaaac agcaggtcgg cattgcaggc cgaggtcagc cacgaattct gctgcagctc
     7621 ggattcgagt tgcccggagg cccagccgga aaagccgaga atcaccaggt agtcctcggg
     7681 tccctggccg ctggcgatcg cctgcaggat gtcgagcgag ctggtcaggt ggatgccgct
     7741 ggatacttcg atgctgttct gccagtgccc gccgccggcg tgcagcacga aaccctgctc
     7801 gagctgcacc gggccgccga tataaaccgg ctggttgcga atgcgctcgt catcgcaatc
     7861 gatattcagc tgctggaaga tatcgtcgag gacctgggaa gtttcgtggt tgacgaccag
     7921 ccccatgctg ccgtcctggt tgtgctcgca catgtagacc aggctggagg cgaaaatggg
     7981 atccttgatt gccggagtcg caatcaggca cttgtcgaca agactggcgg tctgctggtt
     8041 cattcgcagc ttctagctta ctccgctttg ggtttgaaat cgattgcttt tgaagtccca
     8101 ggtgcgaatg atgacgatct ggtcggcgcg ttcgcgcaat tcgtcggaga acggcgcgaa
     8161 cggctgggcc atgcgcagaa tacgcttggc ggcctcgtcg agcacgctgg ttccggatga
     8221 gctggtaatt tccatgttga gcacttcgcc ggccgcgttc aggcgcacgc tcatgcgcag
     8281 cctgccgtcg atattcctgc tgcgggcctc gcgcggatag ttcagattcc cggtacgctc
     8341 gattttctgc acccaggagg cgaggtaact cgcctccacc gccttgcgcg tgctggcggt
     8401 cagggtcagg gtcctgggtc gcttggcgta gtcgat
//
LOCUS       DATCZV010000188         3631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_332217, whole genome shotgun sequence.
ACCESSION   DATCZV010000188 DATCZV010000000
VERSION     DATCZV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332217"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1067
                     /locus_tag="VIW27_04240"
     CDS             234..1067
                     /locus_tag="VIW27_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEY5738907.1"
                     /translation="MSKTLADYISRYQRLFVLAGAGLSTASGIPDYRDERGEWKHSKP
                     MDYRDFVARHQARQRYWARSCIGWPRFQRARPNAAHSALARLETLGRVVVTVTQNVDG
                     LEQRAGCREVIQLHGSLDGVGCLDCQAGISREAMQHRLLAANPELALASAAVLPDGDA
                     ELAFDTGAVRVPECRACGGILKPQVVFFGEAVPAARVAACYRALDEADAVLVLGSSLM
                     VYSGFRFVREAARAGLPIAAINRGKTRADDLLDLKLTQDCAVALNTALDDAGFADAEI
                     A"
     gene            1230..1505
                     /locus_tag="VIW27_04245"
     CDS             1230..1505
                     /locus_tag="VIW27_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738908.1"
                     /translation="MTTVNIHIDPLTTHGVLAQLGQAIEEIKLLRAIDCLKQQLYSGD
                     AVKLDFGRRRYLVAELHHLNQQLQALREQAIGEGCRHGLASSSDIAI"
     gene            1622..2425
                     /locus_tag="VIW27_04250"
     CDS             1622..2425
                     /locus_tag="VIW27_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY5738909.1"
                     /translation="MALNYAAGSLAQRAADLCLMLWPRRRPSRDALEHCRIVSHRGEH
                     DNRRLRENTLAAFAAAADAGSWGVEFDVRWTRDLQPVVIHDPDTARVFGPRIEIAAVE
                     FDELRRHLPEIPRLDEVVRTFGGRQHLMVELKADSIGEDERKAARLAEIFAGLEAGDD
                     YHFLALQESVLEPAGFAGRKACLLVAETNTARISRAVLDGNYGGLCGHYLLIGKRLLA
                     RHRAREQQLGTGFAASRFCFYRELNRGIDWIFTNHARRLAAIRARLLQR"
     gene            2508..3191
                     /locus_tag="VIW27_04255"
     CDS             2508..3191
                     /locus_tag="VIW27_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5738910.1"
                     /translation="MIQDSKGIVEALGKSHLFSALDEAQFDRVRRHSHITDMIEGESL
                     FFQGDEVGCFYLVLEGRIKLYRVSPDGKEKVVEIMETGHTFAEALMFMDEATYPVTAT
                     ALVPSRVIGINCRDFREMLRESVDTCFLLMGKMSYRLRGLIHEIDTLSLDTGTVRTVA
                     YLLRVAPPDQDSFELEIAKSVIASRLSVKPETFSRILRHLHEQDIVSIDGRRVTIHDR
                     DAMIGICSG"
     gene            complement(3202..>3631)
                     /locus_tag="VIW27_04260"
     CDS             complement(3202..>3631)
                     /locus_tag="VIW27_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY5738911.1"
                     /translation="PERVEALRRQLLRWGGNSSGINIANIDNLGNVHPDTMWWDYTLG
                     NVRQRPFSQIWQDTTDPLMAGLKASPRPLEGRCASCRERAICGGNSRTRAWQLTGNPW
                     AEDPGCYLSDAEIGVTDQPGRIGLTPYRRRQEARKLLRRA"
BASE COUNT          604 a         1169 c         1172 g          686 t
ORIGIN      
        1 gaatgttctc gaccagggta tggcgccaga gttcgtggtt gacctcggcg gccggcttgg
       61 aagccctgcc gtggatcatg atgatgcgct tgtcagccag gtcgatggtc atcgaaaagc
      121 cccgtgtcca ggattccggg cccagtttat acgcggtcct cgaaggcaat caatcgcgtg
      181 cgcagatcgt cgccggggct ttccgtgcgc tcagcggctg gtaaactgcg gccatgtcca
      241 aaacgctcgc cgactacatc tctcgctacc agcgcctgtt cgtgctcgcc ggtgccggcc
      301 ttagcaccgc ttccggtata cccgattacc gcgacgagcg cggtgaatgg aaacacagca
      361 agccgatgga ctaccgcgat tttgttgccc gccaccaggc ccgccagcga tactgggcgc
      421 gttcctgcat cggctggccg cggtttcagc gcgcacggcc gaacgctgcg catagcgcgc
      481 tcgcgcggct cgagacgctg ggccgcgtgg tcgtcacggt cacgcaaaat gtcgatggcc
      541 tcgagcagcg tgccggctgt cgcgaagtga tccagctgca cggcagtctc gacggggtcg
      601 gctgcctgga ttgccaagcc ggcatctccc gcgaagcgat gcagcatcgg ctgctggccg
      661 cgaacccgga actggcactg gcgtcggccg ctgtgctgcc cgacggcgat gccgagctgg
      721 cgttcgacac cggcgcggtg cgggtgccgg agtgtcgtgc ttgcggcggg atcttgaaac
      781 cgcaggtggt gttcttcggc gaagccgttc ccgcggcacg cgtcgctgcc tgttaccggg
      841 cactcgacga agccgacgcg gtgctggtgc tcggctcctc gttgatggtt tactcggggt
      901 ttcgtttcgt gcgcgaggcg gcccgcgccg gcctgccgat cgccgcgatc aatcgcggca
      961 agacgcgtgc cgacgatttg ctcgatctca agttgaccca ggattgcgcc gtcgcgctga
     1021 acacggcgct tgacgacgcg ggcttcgcag acgccgagat cgcctgatcg ccggtgtgac
     1081 gcgcgtcggg aaaaattttc gacgcgcgtc acgattccgc ggttttgccc cacccccggc
     1141 atgcgggctc gcgaaattcg gcgaaacggg tcacagtccg aaagcgcgcg gcctcctata
     1201 taccagtcgt taccgcagcg agactaggca tgacaacggt caatattcac atcgatcctc
     1261 tcaccacaca tggcgtgttg gcgcagctcg gtcaggcgat cgaggaaatc aagctgctgc
     1321 gggccatcga ttgcctcaag caacagcttt acagcggcga tgccgtgaaa ctcgatttcg
     1381 gccgccgccg ctacctggtt gccgaactgc accacctgaa ccagcaactg caggcgctgc
     1441 gtgaacaggc catcggcgaa ggctgccggc atggtctcgc cagcagttcc gacatcgcca
     1501 tttaattccc gcacgcattg cactagagtt cacggatgca atgcatccac ccatgtccgt
     1561 gctcgatcac ggtacgcttc cctccacggg gccctcggcg ggcccggcaa cggcgctgac
     1621 cgtggccttg aactatgccg cgggcagcct cgcgcagcgc gccgccgatc tgtgcctgat
     1681 gctgtggccg cgccggcggc cgtcgcgtga cgccctcgag cactgccgca tcgtctccca
     1741 ccgcggcgag catgacaatc gcaggcttcg cgaaaataca ctggcggcgt tcgcggccgc
     1801 tgcagacgcc ggcagctggg gagtcgagtt cgacgtgcgc tggacgcgcg acctgcagcc
     1861 ggtggtgatt cacgatcccg acacggcgcg agttttcggt ccgcgcatcg agatcgccgc
     1921 ggtcgagttc gacgagctgc gccggcacct gccggagata ccgcggctcg atgaggtggt
     1981 gcgaaccttt ggcgggcggc agcacctgat ggtggaactg aaggccgaca gcatcggcga
     2041 ggacgagcgc aaggctgcgc gactggcgga gattttcgcc gggctcgaag cgggtgacga
     2101 ctatcatttc ctggcgctgc aggagagtgt cctcgagccg gccggtttcg ccggccgcaa
     2161 ggcgtgcctg ctggtcgccg agacgaatac cgcgcggatc agccgcgcgg tgctcgacgg
     2221 taactacggt ggactctgcg ggcattacct gttgatcggc aagcggttgc tcgcgcgcca
     2281 ccgcgcccgc gagcagcagc tcggcaccgg ttttgctgcc tcgcgtttct gtttttaccg
     2341 cgagctgaac cgcgggatcg actggatttt taccaatcat gcgcgccgcc tcgccgcgat
     2401 tcgcgcccgc ttactgcagc gctaggactc cgccctgtgc cggactcatg catccgggcc
     2461 agtttcatgt caatatccgt tttcgttgcc ggcaaacgaa aaaaaccatg atccaggatt
     2521 caaaaggtat agtcgaggcc ctcgggaagt cgcacctgtt ctccgcgctc gacgaggcgc
     2581 agttcgaccg cgtgcgtcgt cactcgcaca tcaccgacat gatcgaaggc gaatcgctgt
     2641 ttttccaggg cgacgaggtc ggctgtttct acctcgtgct ggagggccgc atcaagctgt
     2701 accgggtgtc gccggacggc aaggaaaagg tcgtcgaaat catggagacc ggccatacct
     2761 ttgccgaggc gctgatgttc atggacgagg cgacctaccc ggtgaccgcg accgcgctcg
     2821 tgccgagccg cgttatcggc atcaattgcc gcgattttcg cgagatgctg cgtgagtcgg
     2881 tcgacacctg ctttttgctg atgggtaaaa tgagctaccg tctgcgcggc ctgatacacg
     2941 aaatcgatac gctgagtctc gataccggca ccgtgcgcac ggttgcctac ctgctgcgcg
     3001 tggcgccgcc ggatcaagac agtttcgagc tcgaaatcgc caagagcgtc atcgcctcgc
     3061 ggctgtcggt caagccggaa actttctcgc gtattctcag gcacctgcac gagcaggaca
     3121 tcgtgtcgat cgatggccgt cgggttacga ttcacgaccg cgacgccatg atcggtatct
     3181 gctcgggttg agtcgtgcgg ctcaggcgcg ccgcagcagc ttgcgagcct cctgccgccg
     3241 tcggtagggt gtcagcccga tgcggccggg ctggtcggta accccgatct ccgcatcgct
     3301 caggtaacag cccggatcct cggcccaggg attgccggtc aactgccagg cgcgggtgcg
     3361 cgagttaccg ccgcagatgg cgcgttcgcg acagctcgcg cagcgtccct cgagcggccg
     3421 cgggctggct ttcaggccgg ccatcaacgg gtccgtggta tcctgccaga tctgcgagaa
     3481 cgggcgctgc ctgacgttgc cgagggtgta atcccaccac atggtgtccg ggtgcacgtt
     3541 gccgaggttg tcgatgttgg cgatgttgat tcccgacgaa ttgcctcccc agcgcagcaa
     3601 ttggcggcgc agtgcctcga cccgctccgg c
//
LOCUS       DATCZV010000189         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_596865, whole genome shotgun sequence.
ACCESSION   DATCZV010000189 DATCZV010000000
VERSION     DATCZV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596865"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1148
                     /gene="prmC"
                     /locus_tag="VIW27_04265"
     CDS             300..1148
                     /gene="prmC"
                     /locus_tag="VIW27_04265"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY5738912.1"
                     /translation="MAEHIDAAIRWATGQIAACSESPRLDAELLLAHCLDKPRSYLYS
                     WPEKPLDESCWQCFQQLVRQRLQPMPVAYLLGRREFFSLEYAINSAALVPRPETELLI
                     ELALQRIPTAESWRVCDLGTGSGIIAVTVASQRPACRVSACDLDPRCLALARDNADRH
                     GVEVEFFESDWYLGLAPGARFDLILSNPPYIAAGHPFLNQGDLPAEPRLALTPGPTGL
                     EALRTIIAGAPEFLEPGGWLMVEHGYDQQLPVAELMAAAGFVEIRCESDASDLPRASV
                     GRLSGD"
     gene            complement(1176..2276)
                     /gene="prfA"
                     /locus_tag="VIW27_04270"
     CDS             complement(1176..2276)
                     /gene="prfA"
                     /locus_tag="VIW27_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY5738913.1"
                     /translation="MKESLLNKLETLCARHEEIAGLLSDIAVINDQNRFRSLSMEYAQ
                     LEDVVKNFNEFQQAREAIGSVREMLDEDDAEMRKLAEEEVAANNSRLETLEVELQRLL
                     LPKDPADNANVFLEIRAGTGGDEAAIFAGDLFRMYSRYAESQRWQLEVLNSSEGEHGG
                     YKEIIARIIGHGAYSKLKFESGAHRVQRVPETESQGRVHTSAATVAIIPESEEVQMIE
                     INPADLRIDTFRASGAGGQHVNKTDSAIRLTHLPTGTVVECQDERSQHKNKARAMSLL
                     QARLYDDEKQRQQAEQAAERKSLVGGGDRSERIRTYNFPQGRITDHRINLTLYKLEEV
                     MQGSLGQVIDPLMHEYQAEQLASLSEEDGGLN"
     gene            complement(2280..3506)
                     /gene="hemA"
                     /locus_tag="VIW27_04275"
     CDS             complement(2280..3506)
                     /gene="hemA"
                     /locus_tag="VIW27_04275"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5738914.1"
                     /translation="MTAPVDIREKVAFAPEQMQRALVELQEIPAVNESVIVSTCNRTE
                     IYCDASSDCSEMITHWLTAHHGINDDGLSPYIYRHSDQEVARHLFRVASGLDSMVLGE
                     PQILGQLKESYDRARGGNAVNAILDRLFQHSFSVAKRVRTDTEIGSNPVSVAFAAVNL
                     SKQIFGNLDKLHALLIGAGETIELVSRHLRSQQIGSMTIANRSIERARALAEQIGAEA
                     VPINAVPEQLSRADIVISSTAAQLPILGKGATESALKQRKRRPIFMVDLAVPRDIEPE
                     VGEMQDIYLYTVDDLKSVVDENLRGRELAAEAAQEIVDLEVNAFNQWLRTHQSADHIR
                     QLRDSAEMIKHQALEKALAQLRNETDPERALERLANEITNKLLHRPTIEMRKALQSDD
                     QERIELLKFLLGSDSH"
BASE COUNT          578 a         1054 c         1130 g          746 t
ORIGIN      
        1 caagcgtctt gcgcgaatca cggcattacc ggaaaagttg atctcaggga ggtctctgct
       61 gtcgtgaggg agtcaagtgc aaggcgaaat tccacgcaat agcgggagca atacgtaaaa
      121 gtatgcgagc gctttgcgtg gaattttaac gccgcaattg gcttcctcac ggcggcagag
      181 aaaaaggccg tccctggcct acgtgatatc atcaatattc gggggaggag gtattgatgt
      241 ccacggatca tatgtataga ccattttcag aatttccata gtgaaagatt tcataattca
      301 tggctgagca catcgatgct gcgattcgct gggctaccgg gcaaatcgcc gcctgcagcg
      361 aaagcccgcg cctggacgcc gaattgctgc tagcgcactg cctcgataaa ccccgcagct
      421 acctgtacag ctggcctgag aaacccctcg acgagtcttg ttggcaatgt tttcagcagc
      481 tcgtgcggca acgcctgcag ccgatgccgg tggcttatct gttgggccgg cgggagttct
      541 tttcgctgga atacgcgatt aattccgcgg ccctggtgcc gcgaccggag accgagctgt
      601 tgatcgaact cgcgctgcag cggattccga ccgcggaatc ctggcgggtc tgcgatctcg
      661 gtaccggcag tggcattatc gcggtgactg tcgccagcca gcgcccggcc tgccgggtca
      721 gcgcctgtga tctcgatccg cgttgcctgg cactggcgcg tgacaacgcg gatcgccacg
      781 gggtcgaagt cgagtttttt gaatccgact ggtacctcgg cctggcaccc ggcgcgcgtt
      841 tcgacctgat cctgtcgaac ccgccctata tcgccgccgg ccaccccttt ctcaaccagg
      901 gcgatctgcc ggccgagccg cggctcgcgt tgaccccggg gccgaccggg ctcgaagcgc
      961 tgcggacgat cattgccggc gcgcccgaat ttctcgagcc gggcggctgg ttgatggtcg
     1021 agcacggtta cgatcagcag ctgccggtcg cagagctgat ggcggcggcg ggattcgtcg
     1081 agattcgctg cgagagcgat gccagcgatc tgccgcgcgc cagtgtcggg cgcttgagcg
     1141 gggattgatc tcgtctgcga gcggcgcgct gccggtcaat tcaggccgcc gtcctcttcg
     1201 ctgaggctgg cgagttgctc ggcctggtat tcgtgcatca gtggatcgat gacctggccg
     1261 agactgccct gcatgacttc ctcgagcttg taaagcgtca ggttgatgcg gtggtcggtg
     1321 atccggccct gtggaaagtt gtaggtgcga atgcgctcgg aacgatcgcc gccgccgacc
     1381 agcgacttgc gctcggccgc ctgttcggcc tgttgccgct gcttttcatc gtcatagagc
     1441 cgcgcctgca aaagcgacat cgcgcgcgcc ttgttcttgt gttgcgagcg ctcgtcctgg
     1501 cattcgacca cggtgccggt cggcaggtgg gtcagccgaa tcgcggaatc ggtcttgttg
     1561 acgtgctggc cgccggcgcc cgaggcgcga aaggtgtcga tgcgcaggtc ggccgggttg
     1621 atttcgatca tctgcacttc ctcgctctcc ggaatgatcg ctaccgtcgc ggcggaagta
     1681 tgcacacggc cctgcgactc ggtctccggt acgcgctgca cgcggtgcgc accggattcg
     1741 aatttcagct tcgaataggc gccgtgaccg atgatgcggg cgatgatttc cttgtagccg
     1801 ccatgctcgc cctcgctgct gttgagtacc tccagctgcc agcgctgcga ctcggcgtag
     1861 cggctgtaca tgcgaaacag gtcgccggcg aagatcgccg cctcgtcgcc gccggtgccg
     1921 gcgcgaattt ccaggaatac gttggcatta tcggccgggt ctttcggcag cagcagcctt
     1981 tgcagttcga cttcgagggt ttccagtcga ctgttgttgg cggcgacctc ttcctcggcg
     2041 agtttgcgca tttcggcatc gtcctcgtcg agcatctcgc gcaccgagcc gatcgcctcg
     2101 cgggcctgct ggaactcgtt gaaattcttc accacgtcct cgagctgggc gtactccatc
     2161 gacaggctgc gaaagcggtt ctggtcgttg atgactgcga tatcggaaag cagcccggcg
     2221 atttcctcgt gcctggcgca aagcgtttcg agcttgttca gcagggattc cttcatcggt
     2281 taatgggaat cgctgccgag cagaaacttg agcagctcga tacgctcctg gtcgtcactt
     2341 tgcagcgcct tgcgcatttc tatggtcggc ctgtgcagca gcttgttggt aatctcgttg
     2401 gcgagccgct ccagcgcccg ctccgggtcg gtttcgttgc gtagctgggc gagggctttt
     2461 tccagcgcct gatgcttgat catttccgcg ctatcgcgca gttggcgaat gtggtcggcg
     2521 gactgatggg tccgcagcca ctggttgaat gcgttcacct cgagatcgac gatttcctgg
     2581 gccgcctcgg cggccagctc gcggccgcgc aggttttcgt cgaccacgga tttgaggtcg
     2641 tcgacggtgt acagatagat atcctgcatc tcgccgacct cgggctcgat atcgcgcggc
     2701 accgcgaggt cgaccatgaa tatcggccgc cgtttgcgct gcttcagggc cgactcggtc
     2761 gcgcccttgc cgagaatcgg caactgggcg gcagtcgacg aaatcacgat gtcggcgcgc
     2821 gacagctgtt ccggcaccgc attgatcggc accgcctcgg cgccgatctg ctcggccagc
     2881 gcccgggcgc gctcgatgct gcggttggcg atcgtcatcg agccgatctg ctggctgcgc
     2941 aggtgccgcg agaccagttc gatggtttcg ccggcgccga tcagcaacgc atgcagcttg
     3001 tcgagattgc cgaagatctg cttcgacagg ttgaccgcgg cgaaggccac cgacaccggg
     3061 ttggagccga tctcggtatc ggtgcgcacg cgcttggcga cgctgaagga atgctggaaa
     3121 aggcgatcga ggatggcgtt gacggcattg ccgccgcgcg cccggtcgta ggactccttg
     3181 agctggccga gaatctgcgg ctcgccgagc accatcgaat cgaggcccga ggcgacgcga
     3241 aacaggtgac gcgcgacttc ctggtccgag tggcggtaaa tatagggcga caggccgtcg
     3301 tcgttgatcc cgtgatgggc ggtcagccaa tgggtgatca tctcgctgca gtcggaggac
     3361 gcgtcgcagt agatctcggt gcgattacag gtcgatacga tcaccgattc gttgaccgcg
     3421 ggtatctcct gcagttcgac cagcgcccgc tgcatctgct cgggcgcaaa cgcgactttc
     3481 tcgcgaatgt cgaccggagc ggtcaggt
//
LOCUS       DATCZV010000190         7326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_649939, whole genome shotgun sequence.
ACCESSION   DATCZV010000190 DATCZV010000000
VERSION     DATCZV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649939"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VIW27_04280"
     CDS             <1..689
                     /locus_tag="VIW27_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738915.1"
                     /translation="VQGPWHRYGLADAEFSNLGFRINYRTGVKRDRTVNRIALNSADD
                     GQQVVEIGDHVPLVIGHYRFYTSHNKGYAPVFEWLAAGSETPVIGSIHLPAYPINEYR
                     QALEWQVPGSDLQLWTLLRIDENVLPEDRPFEFTVPEAHRIVVRVGEQRFEMAPGERI
                     ELDGGVLGYRGLSSWMGYKIDYDWTRPWLLATAIIAILSLFAHYVIKFARPGTLPSRD
                     SLVSQSSVRA"
     gene            complement(774..1031)
                     /locus_tag="VIW27_04285"
     CDS             complement(774..1031)
                     /locus_tag="VIW27_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEY5738916.1"
                     /translation="MSIATEKFHLGLVAGEALRRLQQQLREWAASAAVKRELRHERRQ
                     LAAMSDTQLRDLGISRADAELEASRVDIPAARIAHQLEYRR"
     gene            1152..2012
                     /locus_tag="VIW27_04290"
     CDS             1152..2012
                     /locus_tag="VIW27_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5738917.1"
                     /translation="MTANRLQTFDLGTLRSFVTIAEAGSMTRAAARLFLTQSAISMQI
                     KRLENSLGFSVFERSPQGMVTTTEGEQLLGFAHRMLALNDEAVGRLTSPDYEGVVRFG
                     VPGDIVYPQIPRVLKEFSRDFPRVQIKFTTLGTFNLLREFDDGKHDIVLTTEQQPRDG
                     GEIIVTHPLVWIGAEDGHAWKKRPLPLGFARHCAFRAGVVAALDEAGIAWLDQVHSER
                     ESAIDAMVAADFCVSARLRMSSIPDARAIDHGGQLPELPDYSIALYCGENRANPIVQT
                     LADYLRRAHG"
     gene            2135..3328
                     /locus_tag="VIW27_04295"
     CDS             2135..3328
                     /locus_tag="VIW27_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5738918.1"
                     /translation="MQRRQVSFLFLNLGHFLDHLFMLIFASVAALRLTSEWGLSYAEL
                     IPYATPGFVAFGLCAVLAGWIADKWSRPGMIVIFFIGIGASSILAGFAQTPLQIAFTL
                     FLIGVFAAIYHPVGLAMVVHERERTGVPLAINGIFGNLGVASAALLTGLLIDSAGWRS
                     AFFVPGVISILVGFTYWLFVRGEPAQATNSSGGPRTKTSAAPTRHVLLRVFAIIFFTT
                     ALGGLIFQSTTFALPKIFDERLGEFAVSATQVGWYAFLVFALAACAQLLIGYLVDRHS
                     VRAIFASVALLQAGFFFAMTRLDGLAALLVAIAFMFAVFGQIPINDVLVGRMARSEWR
                     SRAYALRYVVTFSVMASAVPMIGWVHGSWGFERLFQILAVAALAIFAATLLLPRAERP
                     LPVSA"
     gene            3425..3931
                     /locus_tag="VIW27_04300"
     CDS             3425..3931
                     /locus_tag="VIW27_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY5738919.1"
                     /translation="MKKSIAIILTVTGLALAPMTSQAEEEALFSSQSLTPETALKAAT
                     AALQKCRADGYQVAVAVTDRSGLVQALLRDRFAGAHTIDTATRKAWTSASFRTDTVEI
                     VAFLKDNPQQVGIYQIGGAMMVGGGKVIDAAGSQVGAIGVSGGPNGVIDEVCAAAGVA
                     AIELDIQF"
     gene            3963..4466
                     /locus_tag="VIW27_04305"
     CDS             3963..4466
                     /locus_tag="VIW27_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014108296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY5738920.1"
                     /translation="MKRKRIRRKGRRARFLRFLDDVRRHTPVTGMAVLLVVLWLGFTA
                     AIYVVERGADGTAINSYGRALYWGVAAFSTAGIADTPATDGGRLIGGIWIVIGSVLFF
                     GAIVATVTGYFMRPLQRPHKKIIETIEYNLEQLEDLDPDELDLLRETVDTLIEHVERI
                     KLGNPPS"
     gene            4547..5779
                     /locus_tag="VIW27_04310"
     CDS             4547..5779
                     /locus_tag="VIW27_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5738921.1"
                     /translation="MSFEYSQKSQRLQQALAAFMERHVYANETEFHRQLAQAGKRFAA
                     LPLIEELKQQARAAGLWNLFVPETHGEYSAHGGLSNLDYYPLAETMGRVLWSAEVFNC
                     NAPDSGNMEVLMKYATPEQREQWLQPLLDGEIRSCYAMTEPQVASSDATNIELSMKLE
                     DDEWLLDGRKWFVTGAMNERTRIIIVMGKSDPDNPDRHRQQTQLLVPRDTPGVEVLRA
                     LTTLGYDDAPIGHAEIRFDRVRVPQQNVLLGAGRGFEIAQGRLGPGRMHHCMRLVGAA
                     QRALEITCRRVQSRSTFGRQLARHQSVREDIARSFSEIEMARLLVQKTCVRMDEVGAR
                     DARDLIAACKTSVPLMVQQILDRCMQMHGAAGLSSDFFLAEAFNYARWCRQADGPDQV
                     HQMALGKQIIERYGGEPA"
     gene            5776..6792
                     /locus_tag="VIW27_04315"
     CDS             5776..6792
                     /locus_tag="VIW27_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013783784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5738922.1"
                     /translation="MSTAELDLGALDAYLRERLPQIGHLRGADKFSDGQSNPTFLLHA
                     DSGDYVLRRQPPGELLRSAHAVDREFRVISALEHSEVPVPRALHLCEDRSVLGSLFFV
                     MSHVPGRIFWDPTLPQLDQAERGAVYAEMNRVLAALHDIDIEAAGLADFGRPGNYFER
                     QVSRWSRQYQASEIEPIADMHRLIDWLPRNLPPDDGKVSLIHGDYRIDNMIFDAQGTS
                     ALALLDWELSTLGHPYADLAYQCMQWRMARDCVLPGLGDIDRAALGIPDEQAYVDSYC
                     QRRGLDAIDDWNFYLVFGFFRFAAILQGVLKRAVDGNASSDKAFAYGALAPVLARMAV
                     ELID"
     gene            complement(6789..>7326)
                     /locus_tag="VIW27_04320"
     CDS             complement(6789..>7326)
                     /locus_tag="VIW27_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5738923.1"
                     /translation="HPGRVSKFCAVDIVPTLDVWEQMDAAAAVSQFHWPLLATDAPLP
                     ENLIGQNPEFFYRALLQRWAGSFERLDSRAVAAYLAQYRDPRRIAAQCEDYRAGATVD
                     RINDRIDREAGRRLDCPVLVLWGKGYLGAKARSPLDTWRRWADQVSEIAIDCGHFVVE
                     EEPERALAALREFFREPG"
BASE COUNT         1272 a         2343 c         2361 g         1350 t
ORIGIN      
        1 tggtgcaggg gccctggcat cgctacgggc tcgccgacgc ggaattttcc aacctcgggt
       61 tccgcatcaa ttatcgcacc ggcgtcaaac gcgaccgcac cgtcaaccgt atcgcgctga
      121 attcagccga tgacggccag caggtggttg aaatcggcga ccacgtgccg ctggtgatcg
      181 gccactaccg tttttacacc tcccacaaca agggttacgc accggttttc gaatggctcg
      241 cggcgggatc cgagacgccg gtgatcggca gtattcacct gccggcttac ccgatcaacg
      301 aataccggca ggcgctcgaa tggcaggttc cgggcagcga tctgcagctc tggaccctgc
      361 tgaggatcga cgagaacgtg ctgcccgaag accggccgtt cgaattcacc gtgcccgagg
      421 cgcatcgcat cgtggtgcgg gtcggcgagc agcgtttcga aatggcaccg ggcgagcgca
      481 tcgaactcga cggcggcgtg ctcggttacc ggggattgtc ttcgtggatg ggttacaaga
      541 tcgactatga ctggacccgc ccgtggctgc tggcgaccgc gatcatcgcc atcctgtcgc
      601 tgttcgcgca ctacgtgatc aagtttgcgc gaccgggcac gctgccgtcg cgcgactcgc
      661 tggtgtcgca gtcgagcgtc agggcctgac tcgacgcgcg gctgccggcc cgcaggccaa
      721 ggggtgcgag ccgccggcgg attgcgcagc ggcggcccgc tttggcggcg ccatcaacgc
      781 cgatactcga gttggtgggc aatgcgtgca gccggaatgt cgacccgcga cgcctcgagc
      841 tcggcgtcgg cgcggctgat accgagatca cgcagctgcg tgtcggacat tgctgcgagc
      901 tggcggcgct cgtggcgaag ctcgcgcttc acggctgcgc tggccgccca ctcgcgcagc
      961 tgctgttgca gcctgcgcag cgcctcgccg gcaacgagcc cgagatggaa tttttcggta
     1021 gcaatgctca tcatcatctc ctatcagatt ttgtgaaggc taggggcctt cagttcacgg
     1081 atgttgacta taggtggaat cgagggtata agtaaaacga atatttttga taatcaagtt
     1141 cacaaataat tatgactgcg aatcgcctgc aaactttcga tctcggcacc ctgcgcagct
     1201 tcgtgaccat cgccgaagcc ggcagcatga cgcgcgccgc cgcacgcctg ttcctgaccc
     1261 agtcggcgat cagcatgcag atcaagcgtc tcgagaacag cctcggtttc agcgtcttcg
     1321 agcgctcgcc gcagggcatg gtgacgacca ccgagggcga gcaactactc ggtttcgctc
     1381 accgaatgct ggcgttgaac gacgaggcgg tcggccgcct gacctcgccg gattacgagg
     1441 gcgtggtgcg cttcggcgtt cccggcgata tcgtctatcc gcagataccg cgggtgctga
     1501 aggaattcag ccgcgacttt ccgcgggtgc agatcaagtt cactacgctc ggcaccttca
     1561 acctgttgcg cgagttcgac gacggcaagc acgacatcgt gttgaccacc gaacagcagc
     1621 cgcgcgacgg cggcgagatc atcgtcaccc acccgctggt ctggatcggc gccgaggacg
     1681 ggcacgcctg gaagaaacgg ccgctgccgc tcggcttcgc gcggcactgc gcgtttcgtg
     1741 ccggcgtcgt cgccgctctc gacgaggcgg gtatcgcctg gctcgaccag gtgcactccg
     1801 agcgcgagtc agccatcgac gcgatggtgg cggcggattt ttgcgtcagc gcgcgcctca
     1861 ggatgagcag cattccggat gcgcgggcga tcgatcacgg cgggcaactg ccggagttgc
     1921 cggattactc gatcgcactg tattgcggtg aaaatcgcgc caacccgatc gtgcaaaccc
     1981 tggcggatta cctgcgccgc gcgcacggct gacggctcgc ggagcgtcac cgggagcgcg
     2041 atttcgatag cccgggaccg agcgataggg taatctcaaa gccgccgcga ccaccggcgg
     2101 cgcgggcatt caccatcgag gaggacagca agcggtgcag cgccgacagg tcagtttcct
     2161 gtttttgaac ctcggtcatt ttctcgatca cctgttcatg ctgattttcg cctcggtggc
     2221 ggctctccgg ctgacctcgg aatggggcct cagctatgcc gaactgattc cctacgcgac
     2281 ccccggcttc gtcgcctttg ggctctgcgc ggtgctcgcc ggctggatcg ccgacaagtg
     2341 gagccggccc ggcatgatcg tgattttttt catcggcatc ggtgccagct cgatcctcgc
     2401 cggattcgcg caaaccccgc tgcaaattgc cttcacgctt ttcctgattg gcgttttcgc
     2461 cgccatctac catccggtgg ggctggcgat ggtggtgcac gagcgcgagc gcaccggcgt
     2521 accgctggcg atcaacggca tcttcggcaa tctcggcgtc gccagtgctg ccctgctgac
     2581 cggtttgctg atcgattcgg ccggctggcg cagtgccttc ttcgtgccgg gcgtgatttc
     2641 gattctggtc ggtttcacct actggctttt cgtgcgcggc gaaccggcgc aggcaaccaa
     2701 cagctccggc ggcccgcgga ccaaaacctc ggccgccccg acgcggcatg tgctgctgcg
     2761 cgtgttcgcg atcatttttt tcaccaccgc gctcggcggg ctgatctttc agagcacgac
     2821 cttcgcgttg ccgaagatct tcgatgaacg gctgggcgaa ttcgcggtca gtgcgacgca
     2881 ggtcggctgg tacgcgttcc tggtattcgc gctggcggcc tgcgcgcaac tgttgatcgg
     2941 ttatctggtc gatcgtcact cggtgcgcgc cattttcgcc agcgtggcgc tgctgcaggc
     3001 ggggttcttt ttcgccatga cgcggctcga cggcctcgcc gcgctgctgg tcgcgatcgc
     3061 attcatgttc gcggtgttcg gccagatacc gatcaacgac gtgctggtcg ggcgcatggc
     3121 gcgcagcgaa tggcgcagcc gcgcctacgc gctgcgctac gtcgtcacct tcagcgtgat
     3181 ggcctcggca gtgccgatga tcggctgggt acacggcagc tggggtttcg agcgcctgtt
     3241 tcagatactc gcggtcgcgg cattggcgat attcgccgcc acgctgctgt tgccgcgcgc
     3301 cgagcggccg ctgccggtat cggcctgaag gattttgatc tgcatcaaag ctttaccatt
     3361 aaggggtaaa agccaggcca gggctggtgt attcttgacc gccttacaga gggggaggca
     3421 tatcatgaaa aagtcgatcg cgatcattct taccgtcacc ggtctcgcac tagcgccgat
     3481 gacttcgcag gccgaggaag aagcattgtt ttcgagccag tcgctaaccc ctgaaaccgc
     3541 gctcaaggcg gcgaccgcgg cactgcagaa atgccgcgcc gacggttacc aggtagcggt
     3601 tgcagtcacc gatcgcagcg gtctcgtaca ggcgttgttg cgcgaccgtt ttgccggcgc
     3661 gcataccata gataccgcga cccgcaaggc ctggacctcg gcgagtttcc gtaccgatac
     3721 ggtcgagatc gtcgcgtttc tgaaagacaa tccgcaacag gtcggaattt accagatcgg
     3781 tggcgcgatg atggtcggcg gtggcaaggt catcgacgcc gccggctcgc aggtcggcgc
     3841 gatcggcgtc tccggcggac cgaacggcgt tatcgacgaa gtttgtgcag cagcgggcgt
     3901 tgccgcgatt gaactggata ttcagttcta ggcaaaggtc accaaaccga agcccgccaa
     3961 aggtgaagcg caaacgaatc agacgcaagg gtcgccgggc gcgcttcctg cgttttctcg
     4021 acgacgttcg caggcatacg cccgtcaccg gcatggcggt tttgctggtg gtgctgtggc
     4081 tgggtttcac ggccgcgatt tacgtcgtcg aacgcggcgc cgacggcacg gcgatcaatt
     4141 cctacggcag ggccctgtac tggggcgtgg ccgcgttttc gaccgccgga atcgccgata
     4201 cgccggcaac cgacggcggc cggctgattg gcggtatctg gatcgtgatc ggctcggtat
     4261 tgtttttcgg cgccatcgtc gccaccgtga ccggttactt catgcgcccg ctgcagcgcc
     4321 cgcacaagaa aatcatcgaa accatagaat acaatctcga gcagctcgaa gacctggacc
     4381 cggacgagct ggatttgctg cgcgagaccg tcgacaccct gatcgagcat gtcgagcgca
     4441 tcaagctcgg caacccgccg tcctgaacta agttcgacac gggctgtttc aagcaagact
     4501 ttctcactta taatctgccg gcatagccag cctgcacgga ttcgccatgt cattcgaata
     4561 ctcgcaaaaa tcccagcgcc tgcaacaggc gctggcggct ttcatggagc gccacgtgta
     4621 cgccaacgag accgagttcc accgccagct ggcgcaggcc ggcaagcgtt tcgccgcgct
     4681 gccgctgatc gaggagctca agcaacaggc gcgcgccgcg ggcctgtgga acctgttcgt
     4741 gcccgagacc cacggcgagt acagtgcgca cggcggcctc tccaacctcg attattaccc
     4801 gctggccgag accatgggcc gcgtgctgtg gtcggcggaa gtgttcaatt gcaatgcgcc
     4861 cgacagcggc aacatggaag tgctgatgaa gtacgcgacc cccgagcagc gtgagcagtg
     4921 gctgcagccg ctgctcgacg gcgagatccg ctcctgttac gcgatgaccg agccgcaggt
     4981 ggcgtccagc gacgcgacca atatcgaact gtcgatgaag ctcgaggacg atgaatggct
     5041 gctcgacggg cgcaagtggt tcgtcaccgg ggcgatgaac gaacgcacgc gaatcatcat
     5101 cgtgatgggc aagtccgatc ccgacaaccc cgatcgccac cggcagcaaa cgcagctgct
     5161 ggtaccgagg gacacgcccg gcgtcgaagt gttgcgcgcg ctgaccacgc tcggttacga
     5221 cgacgcgccg atcggtcacg ccgagattcg cttcgaccgc gtcagggtgc cgcagcaaaa
     5281 tgtattgctc ggcgccggcc gcggcttcga gatcgcccag ggccggctcg gtccggggcg
     5341 catgcaccac tgcatgcggc tggtcggtgc cgcgcagcgc gcgctcgaaa ttacctgccg
     5401 gcgagtgcag agccgctcga ccttcggccg ccaactggcc cgtcaccagt cggtcaggga
     5461 agatatcgca cgcagtttca gcgaaatcga gatggcgcgc ctgctggtgc agaaaacctg
     5521 cgtacgcatg gacgaggtcg gcgcccgcga cgcccgcgac ctgatcgcgg cctgcaaaac
     5581 cagcgtaccg ctgatggtgc agcagattct cgatcgttgt atgcagatgc acggcgccgc
     5641 cggcctgtcg agcgatttct tcctcgccga ggcattcaat tacgcgcgct ggtgccggca
     5701 ggcggacggt cccgaccagg tgcaccagat ggcactcggc aaacagatca tcgaacgcta
     5761 cggcggcgaa ccggcgtgag caccgcggaa ctcgatctcg gcgcgctcga cgcctacctg
     5821 cgcgagcggc taccgcagat cggccacctg cggggcgccg ataaattcag cgacgggcag
     5881 tccaatccga ccttcctgct gcatgccgac agcggcgact acgtgctgcg gcgccagccg
     5941 ccgggcgaat tgctcaggtc ggcgcacgcg gtcgaccgcg aatttcgcgt catcagtgcg
     6001 ctcgagcaca gcgaggtgcc ggtaccgcgc gcgctgcacc tgtgcgagga tcgctcggta
     6061 ctcggcagcc tgtttttcgt catgagccac gtgccggggc gcatcttctg ggacccgacg
     6121 ctgccgcagc tcgaccaggc cgagcgcggc gcggtctacg ccgagatgaa ccgcgtgctg
     6181 gcggcgctgc acgacattga tatcgaggcc gcggggctcg ccgatttcgg tcgcccggga
     6241 aattacttcg agcgccaggt cagccgctgg agccgccagt accaggcctc cgagatcgag
     6301 ccgatcgcgg acatgcaccg gctgatcgac tggctgccgc gcaacctgcc gcccgacgac
     6361 ggcaaggtca gcctgatcca cggcgattac cgcatcgata acatgatctt cgacgcccag
     6421 ggcaccagcg cgctggcgct gctcgactgg gaactgtcga cgctcggcca cccttatgcc
     6481 gatctcgcct accagtgcat gcagtggcgc atggctcgcg attgcgtgct gcccggtctc
     6541 ggcgatatcg atcgcgccgc gctcggcatt cccgacgagc aagcctatgt cgacagttac
     6601 tgccagcggc gcgggctgga cgccatcgac gactggaact tttacctggt gttcggcttt
     6661 ttccgcttcg ccgcgatcct gcagggcgta ttgaaacgcg cggttgacgg caacgcgtcg
     6721 agcgacaagg cctttgccta cggcgcgctg gcgccggtgc tggcgcgcat ggcggtggaa
     6781 ctcatcgact agccgggttc acgaaagaat tcacgcagtg ccgccagcgc gcgctcgggc
     6841 tcttcctcga ccacgaagtg gccgcaatcg atggcgatct cgctgacctg gtcggcccag
     6901 cggcgccagg tgtcgagcgg cgagcgggct ttggcgccga gataaccctt gccccacagc
     6961 accagcaccg ggcagtcgag ccggcgaccg gcctcgcgat cgatgcgatc gttgatgcga
     7021 tcgaccgtgg cgccggcccg gtagtcctcg cattgcgccg cgatgcgccg cggatcgcgg
     7081 tactgcgcga ggtaagccgc gaccgcgcgg ctgtcgaggc gctcgaaact gccggcccag
     7141 cgctgcagca gcgcgcggta gaagaattca gggttctgac cgatgaggtt ctccggcagg
     7201 ggtgcgtcgg tggccagcaa cggccagtga aactgcgata ccgcggcggc ggcgtccatc
     7261 tgctcccaga cgtcgagggt cggcacgatg tcgacggcgc agaatttcga cactcggccc
     7321 gggtgg
//
LOCUS       DATCZV010000191         3490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_596890, whole genome shotgun sequence.
ACCESSION   DATCZV010000191 DATCZV010000000
VERSION     DATCZV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596890"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="aroB"
                     /locus_tag="VIW27_04325"
     CDS             <1..786
                     /gene="aroB"
                     /locus_tag="VIW27_04325"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY5738924.1"
                     /translation="VILALGGGVVGDIAGFGAACWQRGVDFMQLPTTLLAQVDSAVGG
                     KTGVNHPLGKNMIGAFHQPRCVVADIGSLNTLPARELSAGLAEVIKYGLIHDAEFFAW
                     LERHIDGLVARDPALLAEAVLVSCRIKARVVELDEREGGLRAILNLGHTFGHAIEAVM
                     GYGSWLHGEAVAAGMVMAIELSIREGWIDESVRQRSVALLERAGLPVAPPAEMSGAQF
                     LDAMAVDKKVQHGRIKLVLLRALGEACITVDYDHDKLLQTLPA"
     gene            1138..>3490
                     /locus_tag="VIW27_04330"
     CDS             1138..>3490
                     /locus_tag="VIW27_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY5738925.1"
                     /translation="MLKGENNKFRQGLYDPQNEHDSCGIGFIADIKNRKSHRIVRDGL
                     TILENLTHRGAVGADPLAGDGAGILIQIPDRLMRAETQALGFELPPPGEYAVAMVFMP
                     QNESTQKECEFALELIANEEGQQVLGWRDVPCDNACLGFSVKPREPVIRQFFIGRGDN
                     CADTAAFERKLFVIRKSAHFRIRKLKPDAWDEFYVASMSASTLLYKGMVLAGNLSKYF
                     LDLRDQRLESALALVHQRFSTNTFPSWELAQPFRFLCHNGEINTVRGNINWMLARKAN
                     MQSDLLGDDLEKLWPLIGDGNSDSATFDNCLELLVAGGYSLAHAVMLMIPEAWLDNPL
                     MDDNRRAFYEYHAALMEPWDGPASVAFTDGRQIGATLDRNGLRPARYLITDDDTVVMA
                     SEMGVLDIPEQKIVKKWRLQPGKMFLIDLEQGRIIDDEELKEQLATAQPYQQWLDDTQ
                     IKLEDVPAEVAAMVPDAATLLDCQQAFGYTREDVDFFLKPMALTGQDPVSAMGRDIPP
                     AVLSHRAKNLFDYFKQNFAQVTNPPIDPIREELVMSLVSLIGPRPNLLDLKSGGKHKR
                     LEVHSPILSSVDLEKIRRIEDFEQSTFRTSTLDIVYPAAEGAAGMEAAIIRLCEKAGK
                     LVKDSGYNILILSDRAVDADFVAIPSLLATGAVHHYLIRQGLRTDVGIVVETGEARRV
                     HDFCLLAGYGAEAINPYLALDSISHIVPEIDDELSVEEAHQRYIKAINKGILKVMSKM
                     GISTYQSYCGAQIFDAVGLRSKFVDKYFAGTATAVEGIGLKEVA"
BASE COUNT          676 a         1133 c         1080 g          601 t
ORIGIN      
        1 gtcatcctgg cgctgggggg cggggtcgta ggcgacatcg ccgggttcgg ggcggcgtgc
       61 tggcagcgcg gcgtggactt catgcagctg ccgacgacgc tgttggccca ggtggactcc
      121 gccgtcggcg gcaagaccgg cgtcaaccac ccgctcggca agaacatgat cggcgccttt
      181 caccagccgc gctgcgtagt cgccgacatc ggcagcctca acaccctgcc ggcgcgcgaa
      241 ctgtcggcgg gactcgccga ggtgatcaag tacgggctga tccacgatgc cgagttcttc
      301 gcctggctgg agcggcatat cgacggcctg gtggcgcgcg atccggcact gctggccgag
      361 gcggtactgg tctcctgccg catcaaggca cgcgtcgtcg aactcgacga gcgcgagggc
      421 ggcctgcgcg cgattctcaa tctcggccat accttcggcc acgcgatcga ggcagttatg
      481 ggctacggca gctggctgca cggtgaagcc gtcgccgccg gcatggtgat ggcgatcgag
      541 ttgtcgattc gcgagggctg gatcgacgaa tcggtgcgcc agcgcagcgt tgcgctgctc
      601 gagcgtgctg gcctgccggt ggcgccgccc gccgaaatga gcggtgcgca gttcctcgac
      661 gcgatggcgg tcgacaaaaa ggtgcagcac ggccgtatca agctggtatt gctgcgcgcc
      721 ctcggtgaag cctgcatcac cgtcgactac gaccacgaca agctgctgca aaccctgccc
      781 gcataatcga tcccggcggc ccggcgcatc cgtcgggccc ggaatgacag gcgtccccct
      841 gttttcgcga gcctcgaatc aacccgctct ccccattttg gtgcatgccg atgtcgatgc
      901 ctgatcgcga tacccgctat acctccagca tgcggacaac tgcgcgccac ggcgccgtaa
      961 accgtgtttc gggctggatt aattgcttat ttctgattat tctggagggt ccccggaacc
     1021 ggcggcctta gccataacga gtgtcgccga aattatcccg acgcagattc catctagcac
     1081 gaccacagaa caatggctcg gggcaaaaga ctgcgcaggg ggctaatgaa ggtgagcatg
     1141 ttgaaaggcg aaaacaacaa attccggcag ggtctgtacg atccgcagaa cgagcacgat
     1201 tcgtgcggca tcggcttcat cgccgatatc aagaaccgca agagtcatcg aatcgtccgc
     1261 gacggactca cgatcctcga aaacctgacg catcgcggcg cggttggcgc ggacccgctg
     1321 gccggcgacg gcgccggcat cctgatccag atccccgacc gcctgatgcg cgcggaaacg
     1381 caggcgctcg gattcgaatt gccgccgccg ggggaatacg cggtggcgat ggttttcatg
     1441 ccgcagaatg aatcgacgca gaaggaatgc gagttcgccc tcgagctgat cgccaacgag
     1501 gaagggcaac aggtcctcgg ctggcgcgac gtgccctgcg acaacgcctg cctcggcttc
     1561 agcgtcaagc ccagggaacc ggtgatccgc cagttcttca tcggccgcgg cgacaactgc
     1621 gccgacaccg ccgccttcga acgcaagctg tttgtgattc gcaagagcgc acacttcaga
     1681 atccgcaagc tgaaacccga tgcctgggac gagttttacg tcgcctcgat gtccgccagc
     1741 acgctgctct acaagggcat ggtgctggcc ggcaacctgt cgaagtattt tctcgatctc
     1801 agggaccagc gcctcgagtc ggcccttgcc ctcgtgcacc agcgcttctc gaccaacacc
     1861 ttcccgagct gggaactggc gcaaccgttc cgcttcctgt gccacaacgg cgaaatcaac
     1921 acggtgcgcg gcaatatcaa ctggatgctg gcgcgcaagg ccaacatgca gtccgacctg
     1981 ctcggcgacg acctcgagaa actgtggccg ctgatcggcg acggcaactc ggactcggcg
     2041 actttcgaca attgtctcga actgctggtc gccggcggct attcgctggc gcacgcggtc
     2101 atgctgatga tcccggaagc gtggctcgac aacccgctga tggacgacaa ccggcgcgcc
     2161 ttctacgaat accacgccgc gctgatggaa ccctgggacg gccccgcgtc ggtggccttt
     2221 accgacggcc ggcagatcgg cgccaccctc gaccgcaacg gcctgcgccc ggcgcgctac
     2281 ctgatcaccg acgacgacac cgtggtgatg gcctcggaaa tgggcgtgct ggatattccg
     2341 gaacagaaaa tcgtcaagaa atggcgcctg cagcccggca agatgttcct gatcgacctc
     2401 gagcaggggc gcatcatcga cgacgaggag ctcaaggagc agctcgccac cgcacagccc
     2461 taccagcagt ggctggatga cacccagatc aagctcgaag acgtacccgc cgaagtcgcg
     2521 gcgatggttc ccgacgcggc caccctgctc gattgccagc aagctttcgg ctacacgcgc
     2581 gaggacgtcg acttcttcct gaaaccgatg gcgcttaccg gtcaggatcc ggtcagcgcg
     2641 atgggccgcg acattccgcc ggccgtactg tcccaccggg ccaagaacct gttcgattac
     2701 ttcaagcaga atttcgccca ggtgaccaat ccgccgatcg atccgattcg cgaggaactg
     2761 gtgatgtcac tggtgtcgct gatcggcccg cggccgaacc tgctcgatct gaagtccggc
     2821 ggcaagcaca agcggctgga ggtgcactcg ccgatcctgt cgagcgtcga cctcgagaag
     2881 atccgccgca tcgaggactt cgagcaaagc accttcagga cttccacgct cgacatcgtc
     2941 tatccggcgg cggaaggcgc ggccggcatg gaggcggcga tcatccgcct gtgcgaaaag
     3001 gccggcaaac tggtcaagga cagcggctac aacatcctca tcctgtcgga ccgcgcggtc
     3061 gatgccgatt tcgtcgcgat cccgtcgctg ctcgccaccg gcgcggtgca tcattacctg
     3121 attcgacagg ggctgcgcac cgacgtcggc atcgtggtcg agaccggcga agcccgccgg
     3181 gtgcacgact tctgcctgct cgccggttac ggcgccgaag cgatcaatcc ctacctcgcg
     3241 ctcgacagta tttcccacat tgtgccggag atcgacgacg agctcagtgt cgaagaagcc
     3301 caccagcgtt acatcaaggc gatcaacaag ggaatcctca aggtgatgtc gaagatgggc
     3361 atttcgacct accagtctta ctgcggcgcg cagattttcg acgcggtggg cctcagaagc
     3421 aagttcgtcg acaagtattt cgccggcacc gcaaccgcgg tcgagggtat cgggctcaag
     3481 gaggtcgccg
//
LOCUS       DATCZV010000192         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_490668, whole genome shotgun sequence.
ACCESSION   DATCZV010000192 DATCZV010000000
VERSION     DATCZV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490668"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VIW27_04335"
     CDS             complement(<1..285)
                     /locus_tag="VIW27_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738926.1"
                     /translation="MKFLRPPNLLIILLPLCSAAAFANPLDEAARLLEETNMARLHEQ
                     QTRPDVVIDEFRSDGCSGGLSDSWKTLARAWPEMARAIGEQPPWESCCVEH"
     gene            complement(424..975)
                     /locus_tag="VIW27_04340"
     CDS             complement(424..975)
                     /locus_tag="VIW27_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HEY5738927.1"
                     /translation="MSSLQEQLLKAGLVDEKKLARANQQKNQQAKQARKQRGKKAKPA
                     NTGRPRPQDNKAERDRELNRQRQQQLRQKELTAQAKQLIVDNLLDRSAGEYSYGFVYK
                     RKVKKIYVTEAQKNQLARGQLAIATHVASDGRRFDLVPAAVADKIAERDASMRVAIDP
                     TQESRRDDDDPYAGYEVPDDLIW"
     gene            complement(1389..2585)
                     /locus_tag="VIW27_04345"
     CDS             complement(1389..2585)
                     /locus_tag="VIW27_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5738928.1"
                     /translation="MNVQWQRRITWLAVLLAIVAALAYSFRPLPRLVDMAPAQRGPMQ
                     VTIEDEGKTRVIDRYIITAPVAGTTCRVDLEVGDPVAKDETLVNINPLQSQALDPRSR
                     AEAQARVAAGEASLHAAEQNVDAARAEADLAGSELARLEPLVEAGHISVERLDQARAV
                     KRGSEAALRSARFAVDVARYELEAARTALRYTGNTSSDPQDIVRVTAPVSGRILKLHQ
                     ECEGVVSRGQPLLEIGDTRSLEIETDVLSADAVKIRPGMPVRYERWGGGDPLGGRVRR
                     VEPVGFTKVSALGVEEQRVLIISDITSDAERWQTLGDGYRVESRFILWEDEDVLQIPG
                     SALFRVDGRWAVFVIDDGEARRREVEIGQRNGLSAQIAQGLNEGEAVITHPDKTLEDG
                     AAVKTR"
BASE COUNT          420 a          897 c          894 g          540 t
ORIGIN      
        1 gtgttcgacg cagcaggatt cccacggcgg ttgctcgccg atcgcacgcg ccatttccgg
       61 ccacgcccgc gccagggttt tccagctatc cgacaggccg ccgctgcagc catcggagcg
      121 aaactcgtcg atgactacgt ccgggcgcgt ctgctgttcg tgcaggcgcg ccatgttggt
      181 ttcctcaagc agccgcgcgg cctcgtcgag cgggttggcg aatgccgccg ccgagcacag
      241 cggcagcagg atgatcaaca ggtttggcgg tcgcaagaat ttcacccggc aaaggtaatc
      301 gacgggcgct tgccgatcaa gcgttggcga atggcggatc gacgagccgg ctgaactcgc
      361 agcggcagat gcgagccgcg acgggcgtcg agcgccgtgg ctgccgggtg ccgccgcgag
      421 cgtctaccag atcaggtcat cgggcacttc gtagccggcg tagggatcgt cgtcgtcgcg
      481 ccgcgattcc tgggttggat cgatggctac ccgcatggac gcatcccgct cggcgatttt
      541 gtcggcgacc gcggctggca ccaggtcgaa tcgtctgccg tcgctggcga catgggtggc
      601 gatagccagc tggcctcgcg cgagctggtt tttctgtgcc tcggtgacgt agattttctt
      661 gaccttgcgc ttgtagacga agccgtagga atattcgccg gctgaccggt cgagaagatt
      721 atcgacgatc aactgtttcg cctgggcggt caactccttc tgccgcagtt gctgttgccg
      781 ctgccgattc agctcgcgat cgcgctcggc cttgttgtcc tgcggccgtg gccggccggt
      841 attcgccggc ttagctttct tgccgcgctg cttgcgcgcc tgcttggcct gctggttctt
      901 ctgctggttg gcgcgcgcca gcttcttctc gtcgaccaga ccggctttga gtaattgttc
      961 ctgtaatgac gacatgcggg gaattgtacc aaagtatcaa ccagccggtt gcgttctcta
     1021 gcaagcgcca gtgatatcgc ccggcggtgt tatcaaccag ctcgaagaca atcgactccg
     1081 gccgacgccc gggtcagata gcgggttgcg ggaagccgca aggaacacac actgatcgcc
     1141 acagcagcgg ttgcggtgat gtcaatgttc gcggccccga tcgccgcggc caggcgggtt
     1201 cttctgtcgc tcgcatccca gtctgcggcc cggttttcga aaaggtctgc cattcatcgg
     1261 tctccaaaat ccaatcggtc ggcattcggc ggacagtcag tatacccatc agccgcggtg
     1321 ccgccagccg cgcgggccgg atcggcgaga ctgcccgcgt aactcgatca cgctgccgcc
     1381 gccggcgttc aacgggtttt caccgcggcg ccgtcttcga gcgtcttgtc gggatgggtg
     1441 atcacggctt cgccctcgtt gagtccctgc gcgatctgcg ccgacaggcc gttgcgctgc
     1501 ccaatctcga cctcgcgccg ccgcgcctcg ccgtcgtcga tgacgaatac cgcccagcgg
     1561 ccgtcgacgc gaaacagtgc gctgcccggg atttgcagta catcctcgtc ttcccagaga
     1621 ataaaacgcg actcgacccg atagccgtcg ccgagggttt gccagcgttc ggcgtcagag
     1681 gtgatgtcgg aaatgattag cacgcgctgt tcttcgacgc ccagcgcgga gaccttggtg
     1741 aagccgaccg gctcgacgcg gcgcactcga ccgccaagcg ggtcaccgcc gccccagcgc
     1801 tcgtagcgca ccggcatgcc gggcctgatt ttcaccgcgt ccgcggagag cacgtcggtt
     1861 tcaatttcca gcgaacgggt atcgccgatt tccagcagtg gctggccgcg gctgaccacg
     1921 ccctcgcatt cctggtgcag cttcagaatc ctgccgctga ccggtgccgt cacgcgtact
     1981 atatcctgcg gatccgacga cgtgttgccg gtgtaacgca gcgcggtgcg cgccgcttcg
     2041 agctcgtacc tggcgacgtc gaccgcgaac cgtgcggagc gcagcgccgc ctcgctgccg
     2101 cgcttgacgg cccttgcctg gtcgagccgt tccaccgaga tatggcccgc ctcgacgagc
     2161 ggttcgaggc gcgccagttc cgacccggcg aggtcggcct cggcgcgcgc ggcgtcgacg
     2221 ttctgttcgg cggcgtgcag cgaggcttcg ccagccgcta cccgtgcctg cgcctcggcg
     2281 cggctgcgcg ggtccagcgc ctgcgattgc agcggattga tgttgaccag cgtctcgtct
     2341 tttgccaccg gatcgcccac ctcgagatcg acgcggcagg tggtgccggc gaccggcgcg
     2401 gtgatgatgt agcgatcgat cacccgggtc ttgccttcgt cctcgatggt gacctgcatc
     2461 ggtccgcgct gtgcgggggc catgtcgacc agtcgcggca ggggcctgaa gctataggcc
     2521 agggcggcga cgatggccag caggaccgcc agccaggtga tgcgtctttg ccattgtacg
     2581 ttcatcgtta ttccttggtt ttcaacactt cgatcagatc gagattgtcc agccggcggc
     2641 gcaccagcag ggccgaagca gcggcggcga gcaacaccac cagcgcggcg aatgcgtagg
     2701 tggacggttc gagcaccagt ggcacgcggt acatttcgtt gccgatgctg g
//
LOCUS       DATCZV010000193         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_332361, whole genome shotgun sequence.
ACCESSION   DATCZV010000193 DATCZV010000000
VERSION     DATCZV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332361"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /gene="malQ"
                     /locus_tag="VIW27_04350"
     CDS             complement(<1..1683)
                     /gene="malQ"
                     /locus_tag="VIW27_04350"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY5738929.1"
                     /translation="MSITDHAALDALCDACGIANGYYDISGSLHQPGIEVKRKLLEAL
                     DYPVSSDADIGRHLETLRSREWRRIIASVMVFRQSGSSLWLRLSLTPAQCAAPVRWEL
                     LEEGGRSRQGSWELQADHASGEYWLDEVPVKRFDVELTDVSDIGYHQLEIKTVDGAAA
                     TSCLILAPGACYEPPAFEHQQKSWGVSLQLYSLRSERNWGIGDFGDLRSAVEILAPMG
                     VDVIGLNPLHALFAHQPEKASPYSPSSRDHLNPVYLDIETIEDYGECPEAQALVTSEG
                     FQANLRSLREEELVNYSGVWAAKLQVLGMLYQSFRHEHLDSGSERAEAFRQFQHDGAE
                     DLFRFALFEALQQYFHAADSNCDRWQQWPLDYQSPDSGLTIEWAERNVAAIEFHQYLQ
                     WNADRQLAEVDDCCRRSGMNIGIYRDLAIGDEKSAAQCWAEPELYAPGMSIGAPPDDF
                     SSSGQNWGLPPLKPPTVNAHFYQQFARTLRANMRHAGALRIDHIMGLMRLYWMPRDCS
                     PDPGSYVAYPLQDLLGITALESQRNRCLVVGEDLGTVPDEVRYALEPNKILSY"
     gene            complement(1674..>2144)
                     /locus_tag="VIW27_04355"
     CDS             complement(1674..>2144)
                     /locus_tag="VIW27_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738930.1"
                     /translation="RGNNNAYCQDNELNWLDWSLLDSNSGLLGMVKRLIQLRNHYAVL
                     HYDRFVHDSRIDWLRDDGGSMTEADWHDPNRQFFAMLLGHAETDSPRKATLVLAFNAA
                     GTRITFELPASPHGWRCIFSTVTSEPDTAAVSRVSLAARSVQVFESGSGEGSCP"
BASE COUNT          450 a          651 c          609 g          434 t
ORIGIN      
        1 gtacgacagg atcttgtttg gttccagcgc atagcgcacc tcatcgggta ccgtgcccag
       61 gtcttctccc accaccaggc agcgattgcg ctgactttcc agcgccgtga tcccaagcaa
      121 gtcctgcaac gggtaggcta cgtagctgcc cgggtccggg gaacaatcgc gcggcatcca
      181 gtaaaggcgc atcaaaccca tgatgtggtc gatacgaagc gcgccggcgt ggcgcatgtt
      241 ggcgcgcagc gttctggcaa actgttggta aaaatgcgcg tttacggtcg gcggtttgag
      301 gggcggcaaa ccccagttct gaccgctcga actgaaatca tcgggcggag caccgatgct
      361 catccccgga gcataaagtt ccggctcggc ccagcattgg gcagcagact tttcgtcgcc
      421 tatcgcgaga tcgcggtaaa tgccgatatt cataccgcta cggcgacaac aatcatcgac
      481 ctcggcgagc tgccgatcgg cattccactg caggtattga tgaaactcga ttgccgcaac
      541 attgcgctct gcccattcga tggttagacc cgaatcgggg gactggtaat cgagcggcca
      601 ttgctgccag cgatcgcagt tcgaatctgc cgcatgaaaa tattgctgca acgcttcgaa
      661 cagggcaaac ctgaacaagt cttcggcacc gtcatgctga aactgtcgaa acgcctccgc
      721 tcgttccgag ccggaatcga gatgctcgtg ccgaaagctc tggtagagca tccccagtac
      781 ctgcagtttc gctgcccaga cgccactgta gttaacgagc tcttcctcgc gaagtgaccg
      841 caggttcgcc tgaaagcctt cgcttgtgac cagcgcctgc gcctccgggc attcgccgta
      901 gtcttcgatc gtctcgatat cgaggtaaac cggattgaga tgatcgcggc ttgatggact
      961 gtagggactc gctttctcgg gctggtgcgc aaacagcgca tgcagggggt taagcccgat
     1021 aacatcgaca cccatcggtg ccagaatctc gaccgccgag cgcagatcgc caaaatcacc
     1081 gataccccag ttgcgctcgg atcgcagcga gtaaagctgc aggctaacgc cccaggattt
     1141 ttgctgatgt tcgaaagcag gcggttcgta acatgccccg ggcgcgagga tcagacagct
     1201 tgtcgccgcg gccccgtcga ccgttttgat ctccagctgg tgataaccga tgtcactaac
     1261 gtcggttagt tcgacgtcga accgctttac cgggacttcg tctagccaat actcgccgct
     1321 tgcatggtct gcctggagct cccaactgcc ctgtcggctg cgcccgcctt cttcgagcaa
     1381 ttcccagcga accggtgcgg cacattgcgc gggcgtcagg cttagcctga gccacaagga
     1441 tgagccgctc tggcgaaaaa ccatgaccga cgcgataatt cgccgccatt cacgggatct
     1501 caaagtttcc aggtgacgcc cgatatcggc gtcgctgctg acagggtagt cgagcgcctc
     1561 gagcagctta cgcttcacct cgataccggg ttggtgcagc gaacccgaaa tatcgtaata
     1621 accgttggcg atgccgcagg catcgcagag ggcatcgagc gctgcgtggt cggttatgga
     1681 catgagcctt ccccagagcc tgattcgaat acctgcaccg accgggctgc gagcgacacg
     1741 cggcttaccg cagccgtatc tggctcactg gttaccgtcg agaagatgca gcgccagcca
     1801 tgcggtgagg cgggtaactc gaaagtaatt cgagtaccgg cggcgttgaa agccagcacc
     1861 agcgtcgcct tgcgcggcga atcggtttcg gcatggccca acagcatcgc gaaaaactgc
     1921 cgattcgggt cgtgccagtc ggcctctgtc atcgacccgc cgtcgtcccg cagccagtcg
     1981 atccggctat cgtgaacgaa acgatcgtag tgcagcaccg cgtaatggtt gcgcaattgt
     2041 atcaggcgtt taaccatgcc cagcagcccg gaattcgaat ccagcaatga ccagtccagc
     2101 caattcagct cattgtcctg gcagtaggca ttattgttac cccg
//
LOCUS       DATCZV010000194         5771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_226930, whole genome shotgun sequence.
ACCESSION   DATCZV010000194 DATCZV010000000
VERSION     DATCZV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226930"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VIW27_04360"
     CDS             <1..1398
                     /locus_tag="VIW27_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY5738931.1"
                     /translation="FDYIGPQMKPSTTITARSSLTDELRQFFVIALPLSAAYLAEFAM
                     FITTKMVVGKLGYQSLAAVGIAGDLSFEVLVILMAMLSIVGVLGAQAHGGGRHDELGR
                     AIRQGFLVGLGIGLPAMLLIWNLDLFMTITGQDTLVVELAQDFLTGLSPAVLPILWFA
                     VLRNFVAVMSRTLPVLVVTSAAVLVNYLLTLWFVHGGFGLPAMGLFGAGLSITLVSWL
                     MFAALAWHIWRQPLYRGYGIFSGKWRLHIATCREIVVLGLPVAGLAFLEAGLFVATAI
                     LSGIIGAKTLAAYEIVIAWAGIPFVIAFGLAEATMIRVAHAVGREDAAERRRAGLMGM
                     GLVLAILSGMLFVPLMFADEIIRIFITPADPGFAEVSALASRFLAISAIFLLFDGLQA
                     AAARALRGMKDNLVPLWIAGFGYWVLGIGGGALLAFHFELGGIGLWFGLAAGLAVAAC
                     LLTLRFHLLTRLDRA"
     gene            1531..2124
                     /locus_tag="VIW27_04365"
     CDS             1531..2124
                     /locus_tag="VIW27_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738932.1"
                     /translation="MKQKLMLVFASALLAVSMATSANQANTETAAEAVPQQAPAAANN
                     FFDPGYWTGAFSANVQQPAISSEITFNAAQPSAWMQWVDPRTHAGMHATFVNPASYMQ
                     FMRPDFYMEFMKPENMAAWMNPAAYQVMMNPQTMSYWMNPATYMHAVDPAMYQQTMNP
                     ANYMAYMNPAAYQQLFESVTCDSENGNQASTWFGIGC"
     gene            complement(2226..3032)
                     /locus_tag="VIW27_04370"
     CDS             complement(2226..3032)
                     /locus_tag="VIW27_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY5738933.1"
                     /translation="MNELIDLERYPLDRLDSPAGERLVECCLAELESGGMFTLKGFLR
                     REAIDAMLDDLQRRMRDESYTHAREHNIYFDDEIDGIPADHPALRRVRTVNHTLCGDQ
                     LAEPLLQVYHWPGMTDFLARVMNKTALFPMADPLACANVLGYYAGEGLNWHFDRSEFT
                     TTLLLQAAERGGEFQYCKALRSDSDPNYDGVARLLRGEDERVSTLTLGAGDLNVFKGK
                     NTAHRVTAPHGERPRLVTVFSYYETPGRLFSDRENLGFYGRTAASAARFE"
     gene            complement(3040..3372)
                     /locus_tag="VIW27_04375"
     CDS             complement(3040..3372)
                     /locus_tag="VIW27_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738934.1"
                     /translation="MSNAEQGISQAQTEAVRGQLVEKDGLRSAMSTNIVYQNERIIVY
                     SLAFCMLGLLVVWATASSPLLLHGSLALVILLTLLWGLARIAGIERKRAERAQQAAEF
                     ESANQRFE"
     gene            complement(3471..4082)
                     /locus_tag="VIW27_04380"
     CDS             complement(3471..4082)
                     /locus_tag="VIW27_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009723291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY5738935.1"
                     /translation="MNQQTINRHLDRLAKNDPELRRGLAQVGYPPPRIRPPGFETLLS
                     TIVSQQISTAAAAAIMQRLHALLPNMAPAALLDLPRGALRAAGLSSPKVAYAEDLARA
                     VIDGRVAIDRFDDMEDAAVVEAITSLKGFGTWSAEIYLMFSLRREDVFPAGDLALRSA
                     LQRLRGLNEKLSEKRARELIADWSPYRSAGSLFLWHFYHGTPL"
     gene            4199..4483
                     /locus_tag="VIW27_04385"
     CDS             4199..4483
                     /locus_tag="VIW27_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007427410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY5738936.1"
                     /translation="MAAYWIAHVEVTDPDAYGEYAKRATDAIEAHGGEFLARGSRTVW
                     LEGRERARNVVVRFPSVEDAERCYASAAYQEALCFARDAADRDLCIVEGS"
     gene            4493..5299
                     /locus_tag="VIW27_04390"
     CDS             4493..5299
                     /locus_tag="VIW27_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5738937.1"
                     /translation="MPLLDQASVEAFQRDGVVLLAGVFRDWVETLRAGVERNMAEPGR
                     YGREYIDGEQGRFFGDYCNWNRIGEYRRFLFDSPAAEIAADLMQSSGTRLFHEHVLVK
                     EPGTDKVTPWHHDQPYYCVDGRQVCSLWIPLDPVARETCPEFIAGSHDWGRWFLPRKF
                     SGIDYDHDDARLEAVPDIDADRDTYDIRSWALEPGDAIAFHFLTLHGAPANRSPQRRR
                     GFAARWLGDDAVFAHRSGEISPPFPGLEQRLQPGDPLDTEEFPLVFPRPV"
     gene            complement(5513..>5771)
                     /locus_tag="VIW27_04395"
     CDS             complement(5513..>5771)
                     /locus_tag="VIW27_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEY5738938.1"
                     /translation="GMLGTLSAGIFVAAGLGGAGLADGVGIGQQLGVQALGIAVTVGW
                     TALVTFVILKIVGALVELRVSEEDETMGLDLTAHEESGYNL"
BASE COUNT         1017 a         1835 c         1787 g         1132 t
ORIGIN      
        1 ttcgactaca ttggccctca gatgaaaccg tctaccacca ttaccgcgcg ttcgagcctg
       61 acggacgaac tcaggcagtt tttcgttatc gcgctgccgt tgtcggccgc ctatctcgcc
      121 gagttcgcaa tgttcattac caccaagatg gtggtcggca agcttggcta ccagtcgctg
      181 gccgcggtcg gcattgccgg cgacctgtcc ttcgaggtgc tggtgatcct gatggcgatg
      241 ctgtcgatcg tcggcgtgct cggcgcgcag gcacacggcg gcgggcggca cgacgaactg
      301 gggcgcgcaa ttcgccaggg attcctggtc ggcctcggca tcggcctgcc ggcgatgctg
      361 ctgatctgga acctcgacct gttcatgacc atcaccggcc aggacacgct ggtcgtcgag
      421 ctggcgcagg atttcctgac cggtctgtcg ccggcggtgc tgccgatatt gtggttcgcg
      481 gtgctgcgca atttcgtcgc cgtgatgtcg cgcaccctgc cggtcctggt ggtaaccagc
      541 gccgcggtac tggtcaacta cctgttgacg ctgtggttcg tgcacggcgg tttcggcctg
      601 ccggcaatgg gcctgttcgg cgccgggctg tcgatcacgc tggtctcctg gttgatgttc
      661 gcggcgctgg cgtggcatat ctggagacag ccgctgtacc gcggctacgg gatattcagc
      721 ggcaaatggc gactgcacat tgcaacctgc cgggaaatcg tggtactcgg actgccggtt
      781 gccggcctcg cgtttctcga ggcgggactg ttcgtggcca ccgcgatact atcgggcatc
      841 atcggcgcga aaaccctcgc cgcctacgag atcgtcatcg cctgggccgg gattccgttc
      901 gtcatcgcct tcgggctcgc cgaggcgacg atgattcgcg tcgcccacgc ggtcggtcgc
      961 gaggatgccg ccgaacggcg ccgcgccggc ttaatgggca tgggcctggt actggcaatc
     1021 ctgtccggca tgctgttcgt gccgctgatg tttgccgacg agatcattcg catttttatc
     1081 actcccgcgg atcccggttt tgccgaggta tccgcgctgg catcacgctt cctcgcgatc
     1141 tcggcgatct tcctgctgtt cgacggcctg caggcagccg ccgcgcgcgc gttgcgcggc
     1201 atgaaggaca acctggtgcc gctatggatc gcgggcttcg gctactgggt gctcggcatc
     1261 ggcggcggcg cgctgctggc ctttcatttc gaactcggcg gcatcggcct gtggttcggc
     1321 ctggcggccg ggctcgcggt tgccgcctgc ctgctgaccc tgcgctttca cctgctaacg
     1381 cgactggatc gcgcctgaat cacggcgcgg gcacatatct taccaagggg atagatgcta
     1441 atacttcata agtatattag ttaatactta tatgtatgat aatcgccgcc gaattacggc
     1501 taatttcaat taacatcttg gggaataaac atgaaacaaa aattgatgct cgtcttcgcg
     1561 tccgcactgc tcgcggtcag catggcaacc agcgccaacc aggcaaacac cgaaaccgct
     1621 gccgaggcag tgccgcagca ggcacctgcg gccgccaata attttttcga ccccggttac
     1681 tggaccggcg ctttttccgc caacgtgcag cagccggcga tttccagcga aatcaccttc
     1741 aacgccgcac agccttcggc ctggatgcag tgggtcgacc cgcggacgca cgccgggatg
     1801 catgcgacct tcgtcaaccc ggccagttac atgcaattca tgcgtcctga tttctacatg
     1861 gaattcatga aaccggaaaa catggcagcg tggatgaatc cggccgcgta ccaggtgatg
     1921 atgaacccgc aaaccatgag ctactggatg aacccggcaa cctacatgca cgccgtcgat
     1981 ccggcgatgt accagcaaac gatgaatccg gcgaactaca tggcttacat gaacccggcc
     2041 gcctaccagc agctgttcga gtctgtgact tgtgacagcg aaaacggcaa ccaggcttcg
     2101 acctggttcg gaatcggctg ctagaccctc ccaaatacct gttaataggg tatttctcca
     2161 cgcttgaccg cggtctctgg ccgcgggttt tttttgcgcg ctgaaagcct gcctgccgaa
     2221 cgcggctatt cgaagcgcgc ggcggaggcg gccgtgcgac cgtagaaacc caggttttca
     2281 cgatcggaga acagccgccc gggggtttcg tagtacgaga acacggttac cagacgcggc
     2341 cgctcgccgt gcggcgcggt cacgcggtgc gcggtattct tgcccttgaa gacgttgagg
     2401 tcaccggcgc cgagggtcag cgtcgacacc cgctcgtcct cgccgcgcaa caggcgcgcg
     2461 acgccgtcgt agttgggatc gctgtcgctg cgcagcgcct tgcagtactg gaattcgccg
     2521 ccccgttccg ccgcctgcaa caatagcgtg gtggtgaact cggagcgatc gaagtgccag
     2581 ttgagaccct cgcccgcgta atagccgagc acgttcgcgc aggccagcgg gtcggccatc
     2641 gggaagagag cggtcttgtt cattacccgc gccagaaaat ccgtcattcc aggccagtgg
     2701 taaacctgca gcagcggctc ggcgagctgg tcgccgcaca gcgtgtggtt gacggtgcgc
     2761 acacgcctga gcgccgggtg gtcggcggga ataccgtcga tctcatcatc gaaataaata
     2821 ttgtgttcgc gcgcgtgggt gtaggactcg tcgcgcatgc gacgctgcag gtcgtcgagc
     2881 atcgcgtcga tcgcttcgcg gcgcagaaaa cctttgagcg tgaacatgcc gccgctttcg
     2941 agttcggcga ggcagcactc gaccaggcgc tcgcccgccg ggctgtcgag ccggtccagc
     3001 gggtagcgct cgagatcgat caattcattc atctgcgtat tactcaaacc gctggttggc
     3061 cgattcgaat tccgcggcct gttgcgcgcg ctcggcacgc ttgcgttcga ttccggcgat
     3121 acgtgccagc ccccacaaca aggtcagcaa gatgaccagc gcgagcgaac catgcaacaa
     3181 cagcggcgag cttgccgtcg cccagaccac cagcagcccg agcatacaaa aggccagtga
     3241 ataaacgatg atgcgttcgt tctggtagac gatatttgtt gacatcgccg agcgcaggcc
     3301 atctttttcg accagttggc cgcgtaccgc ctcggtttgc gcttgcgaaa tgccctgttc
     3361 tgcgttcgac atatcgaccc ccgccggatt ttcgacgatt aactatattc ctgcctcgcg
     3421 ggcccggcaa gcggtgattt atcaaccgcg atacggtgct ttctcgccgc tcacagcggc
     3481 gtgccgtgat agaagtgcca caggaacagg ctccccgcgc tgcggtacgg cgaccagtcg
     3541 gcgattaatt cgcgggcgcg cttttcgctg agtttctcgt tcagccccct gagccgctgc
     3601 agcgcgctgc gcaaggccag gtcgccggcc gggaacacgt cctcgcggcg cagcgagaac
     3661 atcaggtaaa tctccgcgct ccaggtgcca aagcctttca gcgaggtgat cgcctcgacc
     3721 accgccgcat cctccatgtc gtcgaagcgg tcgatcgcga cgcggccatc gatcaccgcc
     3781 cgcgccagat cctcggcata agcgaccttg gggctggaca ggccagccgc gcgtaacgcg
     3841 ccgcgaggca gatccagcag tgccgcgggc gccatgttcg gcagcagcgc gtgcaggcgc
     3901 tgcatgatcg cggccgcggc ggcggtcgaa atctgttgcg agacgatagt cgacaacagg
     3961 gtttcgaagc ccggcggacg aatgcgcggc ggcggatagc cgacctgcgc gagcccgcgt
     4021 cgtagttcgg gatcgttctt ggcaagcctg tcgaggtgcc ggtttatcgt ttgctggttc
     4081 atcgctaatt cggttgcgaa gcggcatcct gtcgagttcg accacgaagc ggtttgcttg
     4141 atggcccgaa gcccctaata tatcaggcca ccgtcaatct catcaggagg ttctgccaat
     4201 ggcagcttac tggatcgccc acgtcgaagt caccgatccc gacgcttacg gcgaatatgc
     4261 aaaacgcgcc accgatgcca tcgaggcgca tggcggtgag tttctggcac gcggctcgcg
     4321 caccgtctgg ctggagggcc gcgaacgcgc gcgtaacgtc gtggtgcgct ttccgagcgt
     4381 cgaggacgcc gagcgctgct acgcgtccgc ggcttaccag gaggccctgt gtttcgcccg
     4441 cgatgccgct gaccgcgatc tgtgcatcgt cgagggcagc tagcgaattc cgatgccgtt
     4501 actggatcag gcaagcgtcg aggcttttca gcgcgacggc gtggtactgc tcgcaggagt
     4561 attccgagac tgggtcgaga cgctacgcgc cggcgtcgag cgcaacatgg ccgagcccgg
     4621 ccgttacggc cgcgaatata tcgacgggga acagggccgt ttcttcggtg attattgcaa
     4681 ctggaaccgc atcggcgagt accgtcgttt cctgttcgac tcgccggcgg cggaaatcgc
     4741 cgccgacctg atgcaatcga gcggcacgcg cctgttccac gagcacgtgc tggtcaagga
     4801 gcccggcacc gacaaggtaa cgccgtggca ccacgaccag ccgtattact gcgtcgacgg
     4861 ccgccaggtc tgcagcctgt ggattccgct cgatccggtg gcccgcgaaa cctgccccga
     4921 gttcatcgcc ggttcgcacg actgggggcg ctggttcctg ccgcgcaagt tcagcggcat
     4981 cgactacgac cacgacgatg cgcggctcga agcggtgccg gatatcgacg ccgaccgcga
     5041 tacttacgat attcgcagct gggcactcga gccgggcgat gcgatcgctt ttcatttttt
     5101 gacgctgcac ggcgcaccgg ccaatcgttc gccgcagcga cgacgcggtt tcgccgcgcg
     5161 ctggctcggc gacgacgcgg tatttgcgca ccgcagtggc gagatttcgc cgccttttcc
     5221 gggactggag caacgcctgc aacccggcga cccgctcgat acggaagaat ttccgctggt
     5281 ctttccccgc cccgtctgaa agcgaattcg cgggtttcga ggaccctgga acgaacgcgc
     5341 gcctgtttcc cggctggcgc cgcgattcga ttggcgaact tctgtcgccg gcgaagggag
     5401 cgcagctcgt caacgctcag gggacggcga acagagactt cgcaatcggc tgaacacgat
     5461 acccgtttac ctctatatca atccgggtga acgggtatcg tgccccgggt tatcagaggt
     5521 tgtaaccgct ctcctcgtgc gcggtcaggt cgagacccat ggtttcgtct tcctcgctga
     5581 cacgcagttc gacaagcgcg ccgacgatct tgaggatcac gaacgtcacc agcgccgtcc
     5641 agccgacggt caccgcgatg cccagcgcct gcacgcccag ctgctggccg ataccgacac
     5701 cgtcggcgag acccgcgccg ccgagcccgg cggcgacgaa gatgcctgcc gacagcgttc
     5761 ccagcatgcc g
//
LOCUS       DATCZV010000195         4866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_438392, whole genome shotgun sequence.
ACCESSION   DATCZV010000195 DATCZV010000000
VERSION     DATCZV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438392"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1344)
                     /locus_tag="VIW27_04400"
     CDS             complement(370..1344)
                     /locus_tag="VIW27_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5738939.1"
                     /translation="MTRPKPAYVYDPGIEIANLLQHRDAQHVNPANSRNIPPPGEPGR
                     VLIASWNVANLGVHKRRDIDLRVIAEILGWFEIVALQEIADNLDDFLAVAAHLPPYFD
                     YIFNDRAGNDERSAYLFDTRRVRLSAKIGEVVVVDSDRKYISLPGIERRFTGFNRNPY
                     LAGFEIENTALLLANCHLLYGSSRSAAERLASIERRQLEAYAIARWCDLRRNDKHHWT
                     RNILALGDFNLPLAQAGDPIHDALTRRGLSLPPHETRIPTNVSGNADYDQIAVTPGLK
                     SKVRKMGVFDFDRVLFADIYDAERSAYWRRCAKYYVSDHRPLWMQLEL"
     gene            1513..1926
                     /locus_tag="VIW27_04405"
     CDS             1513..1926
                     /locus_tag="VIW27_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HEY5738940.1"
                     /translation="MARRDFTFTSFLLRLLFAALLVFLTYNPSGYSWVGWLDSGVPMV
                     YKVAGGIVLLIGWMMFVRATWNSLGAIGTVLAAAFFVVIIWLLIEWNIVSLSSAAVFQ
                     WVILVILTGVLAVGMSWSHVRRRLSGQYDTDEIEG"
     gene            1964..2617
                     /locus_tag="VIW27_04410"
     CDS             1964..2617
                     /locus_tag="VIW27_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5738941.1"
                     /translation="MPGLSDNVILLHGLARTDRSMHKLEQALLEQGYCVDNVRYESTR
                     HDIETLSEQAIGAALATCPAGQPVHFVTHSLGGILVRQYLENHRIENLGRVVMLGPPN
                     KGSEVVDKLAEFPGFHFINGDAGLQLGTGATSVPNRLGRADFDLGIIAGTRSVNLLLS
                     SIIPGADDGKVSVENTRLEGMRDHLEMPVTHTFMMQNTEVIEQVIHYLRFGEFRRPA"
     gene            complement(2709..3152)
                     /locus_tag="VIW27_04415"
     CDS             complement(2709..3152)
                     /locus_tag="VIW27_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY5738942.1"
                     /translation="MADRVADGKIEHGSFFENARARAFEYLRNPGKLNRLLDRATREI
                     YRRQEAFAGLRDSLQTCLRLLRAYADGRYRGIPAASLVSIVAWLIYFVMPVDSIPDFI
                     LALGLVDDAALLGWVLSSVRTDLDRFVEWENRVAGEPAKSALGKN"
     gene            complement(3163..4017)
                     /locus_tag="VIW27_04420"
     CDS             complement(3163..4017)
                     /locus_tag="VIW27_04420"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEY5738943.1"
                     /translation="MKILMIPIHRILLAAALLAVAGCSLPRFAYQQAPNYVAGKLDEA
                     FDLDEVQSAALDERLQRFFAWHREQELRQYRAVLQRAARDSSDGIRAEEFLRVHDEIR
                     AAWRRAVDKAIDNVGDLALTLTPAQIASYEAYYEERSERYREYQEMSVQQREDYRLTR
                     NLERLEQWLGDFDEFVRDRVSDRLRQLPDNYESWIRYRDARHRELVAALQDAQRTGLD
                     TTRLKAILIGPDTEYARAYAPARQAYWQAYAEALEDISGWIDTRQRARLVSRLEDYAR
                     IVDDLVSS"
     gene            complement(4059..>4866)
                     /gene="ispH"
                     /locus_tag="VIW27_04425"
     CDS             complement(4059..>4866)
                     /gene="ispH"
                     /locus_tag="VIW27_04425"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5738944.1"
                     /translation="VDGLRDRGAIFVDQLDEVPDGATVIFSAHGVSQAVRSEAERRRL
                     KVFDATCPLVTKVHMEVSRYSRQGIETVLIGHRGHPEVEGTMGQYDRAAGGDIYLVES
                     AADARRLQPRNPEQLRFVTQTTLSKDDTGIVIDTLQQVFPKIRGPKKEDICYATQNRQ
                     DAVKQLAERCDLILVVGSRNSSNSNRLREIAENRGIEGHLIDDESDIRPEWLRGKQCV
                     GVTAGASAPELLVQNVVEFLQQHGAGEATSLGFTEDVIFPLPRPLREATG"
BASE COUNT          806 a         1487 c         1604 g          969 t
ORIGIN      
        1 ttgcccgtcc cgggacgatc cgcagcgggc ggccctcggc gatgccgtga atggattgcg
       61 gctcggggcc gcaatgaccg cgccggccgc gttgccgcga attccccgtc atgccggccc
      121 tcgagccggc atcccaatac gcccggcaat agtgcccgtt ccgggactat ccgcagcggg
      181 cgaccacctc ggtaaagccg ttaatggatt gcggctcggg gccgcaatga ccgcgccggc
      241 cgcgttgccg cgaattcccc gtcatgccgg ccctcgagcc ggcatccatg ccggtagtgg
      301 cgaggttccc atgccgggca gcccgatttt ccgggtatgg ctccgcgacc ggaatcgcac
      361 tggacctgct cagagttcca gctgcatcca cagcggacga tgatccgaga cgtagtactt
      421 ggcgcagcga cgccagtagg cggatctttc cgcatcgtag atgtccgcga acagcacgcg
      481 gtcgaaatcg aaaacaccca tcttgcgtac cttcgacttc agacccggcg tcaccgcgat
      541 ctggtcgtag tcggcgttgc ccgagacatt ggtcggaatc cgggtttcgt gcggcggcag
      601 gctcaggccg cggcgcgtca gcgcgtcgtg aatcgggtcg ccggcttgcg ccagcggcag
      661 attgaaatca cccagtgcca ggatattacg cgtccagtgg tgcttgtcgt tgcgccgcag
      721 gtcgcaccag cgcgcgatcg cgtacgcctc cagctggcgg cgttcgatcg atgccagccg
      781 ctcggccgcc gagcggctgg aaccgtaaag caggtggcag ttggcgagca gcagggcggt
      841 attctcgatc tcgaaaccgg ccaggtaggg gttgcggttg aagccggtga agcggcgctc
      901 gatgccgggc aagctgatgt atttgcggtc gctgtcgacc accacgactt cgccgatctt
      961 ggcgctcagc cgtacacgcc gggtgtcgaa caggtaggcc gagcgctcgt cgttgccggc
     1021 gcgatcgttg aagatgtaat cgaagtaagg cggcaaatgg gcggcgacgg cgaggaaatc
     1081 gtcgaggttg tcggcgatct cctgcagcgc gacgatttcg aaccagccga ggatctcggc
     1141 aatcacgcgc aggtcgatgt cgcggcgctt gtgcacgccg agattggcga cattccagct
     1201 ggcgatcaat acccggcccg gctcccccgg cggcggaatg ttgcggctgt tcgccgggtt
     1261 gacgtgctgt gcgtcgcggt gttgcagcag gttggcgatc tcgatgccgg gatcgtagac
     1321 gtacgcgggt ttcggtctag tcaatgatcg catccatcgt ctcctggtat ccggtgaatg
     1381 gccgctgctg ccgccctgcg gatttccggc cggcccgggc acagtttact gccatgacgg
     1441 gcgcttgtga atttcacgcg gcgacgataa tgtctgctaa cataacgttt ttatcaccat
     1501 tgccgaatcc agatggcccg tcgagacttc actttcacca gtttcctgtt gcggctgtta
     1561 ttcgccgcgc tgctggtatt tctgacctac aacccgagtg gttattcctg ggtcggctgg
     1621 ctcgattccg gggtgccgat ggtctacaag gtcgccggcg gtatcgtgct gctgatcggc
     1681 tggatgatgt tcgtgcgcgc gacctggaat tcgctcggcg cgatcggcac ggtgctggcg
     1741 gcggcgttct tcgtcgtcat catctggctg ctgatcgagt ggaatatcgt gtcgctgagc
     1801 agtgccgccg ttttccagtg ggtgatcctg gtaatcctta ccggggtgct ggcggtgggc
     1861 atgtcctggt cgcacgtgcg gcgacggtta tcgggacagt acgacaccga tgaaatcgag
     1921 ggttgaggcg ctcggcctgg cgctggtgct gtggattctg ccaatgcccg gcctcagcga
     1981 taacgtcatt ctgctgcatg gcctggcgcg caccgatcgc tcgatgcaca agctcgagca
     2041 ggccctgctg gagcagggat actgcgtcga taacgtgcgc tacgaatcca cgcgtcacga
     2101 tatagaaacg ctgtcggagc aggctatcgg tgccgcgctt gccacctgcc cggcgggaca
     2161 gccggtgcat ttcgtgaccc attcgctggg cggtattctg gtacgccagt atctcgaaaa
     2221 ccatcgcatc gagaatctcg gccgcgtggt catgctcgga ccgcccaaca agggcagcga
     2281 ggtggtcgac aaactggccg agtttccggg ttttcacttc atcaatggcg atgccggact
     2341 gcaactcggc accggggcga ccagcgtgcc gaaccggctc ggccgagccg acttcgatct
     2401 tggcatcatc gccggtacgc gcagtgtcaa tctgctgttg tcgagcatca taccgggtgc
     2461 cgacgacggc aaggtttcgg tcgaaaacac gcgcctggaa ggaatgcgcg atcatctcga
     2521 gatgccggtg acccatacct tcatgatgca gaataccgag gtgatcgagc aggtgatcca
     2581 ttacctgcgt ttcggcgaat tcaggcgccc ggcctagccg gcgagattat tcggtttttg
     2641 accgcggctg gcggagagga agggggctgg cgaggaacag cgctgaatgt cgccagccac
     2701 cggtgtcgtc aatttttgcc gagcgccgat ttggccggct cgccggcaac gcgattttcc
     2761 cactcgacga agcgatcgag atcggtcctg accgacgata gcacccagcc gagcagggcg
     2821 gcatcgtcga ccaggcccag cgcgagaatg aaatcgggta tcgagtcgac cggcatgacg
     2881 aaataaatga gccaggcgac gatcgacacc aggctggcag ccggaatccc ccggtaacga
     2941 ccgtcggcgt aggcgcgcag cagtctcagg caggtttgca gtgaatcgcg aagccccgcg
     3001 aatgcctcct ggcgacggta gatctcgcga gttgcccggt cgagcagccg gttgagtttg
     3061 ccggggttgc ggaggtactc gaaggcgcgc gcgcgggcgt tttcgaagaa cgagccgtgt
     3121 tcgatttttc cgtccgcaac cctgtcagcc atcggttcct gcctagctgc tcacgagatc
     3181 gtcgacgatg cgcgcatagt cctcgagcct ggagaccagg cgcgcccgct gccgcgtgtc
     3241 gatccagccg ctgatgtctt ccagtgcctc ggcataggcc tgccagtatg cctgccgcgc
     3301 gggtgcgtag gcgcgcgcgt actcggtgtc cgggccgatc aggatggctt tcaggcgggt
     3361 ggtatcgagg ccggtccgct gggcgtcttg cagcgccgcg accagctccc gatggcgggc
     3421 gtcgcgatat cgtatccagg actcgtagtt atcgggcagc tgccgcaggc gatcgctgac
     3481 gcggtccctg acgaattcgt cgaaatcgcc caaccattgc tcgagccgtt cgaggttgcg
     3541 tgtcaggcga tagtcttcgc gctgctgcac gctcatctcc tggtattcac ggtatctctc
     3601 ggaacgttct tcgtagtaag cctcgtaact ggcgatttgc gccggggtca gggtcagcgc
     3661 gagatcaccg acattgtcga tggctttgtc caccgcacgc cgccaggcag cgcggatctc
     3721 gtcgtgcacg cgcaggaact cctcggcgcg aatgccgtcg ctgctgtcgc gggcggcgcg
     3781 ttgcaggact gcgcggtact ggcgtaattc ctgttcgcgg tgccaggcga aaaatcgctg
     3841 caggcgctcg tccagcgccg cactttgcac ctcgtcgagg tcgaaggctt cgtcgagctt
     3901 gccggcgaca tagttcggcg cctgctggta ggcgaacctg ggcagactgc aacccgccac
     3961 ggccagcagt gccgcggcga gcaggattcg gtggatcggt atcatcaaga ttttcatacg
     4021 tcgcttcgat tcgcctggac cagccctcgt cacacggctc agccggtggc ctcgcgcagc
     4081 ggccggggca gcggaaagat cacgtcctcg gtaaagccca gcgaggtcgc ttcgccggcg
     4141 ccgtgttgtt gcaggaattc gaccacgttt tgcaccagca gttcgggtgc cgaggcaccc
     4201 gcggtcacgc cgacgcattg cttgccgcgc agccactcgg gacgaatatc ggattcgtcg
     4261 tcgatcaggt gaccctcgat accgcggttc tcggcaattt cgcgcaggcg gttggaattc
     4321 gagctgttgc gcgaaccgac caccaggatc aggtcacagc gctcggcgag ctgcttgacg
     4381 gcatcctggc ggttttgcgt ggcatagcag atgtcttcct ttttcggccc gcgaatcttc
     4441 ggaaacactt gctgcagggt gtcgatgacg atccccgtat cgtccttgga cagcgtggtc
     4501 tgggtgacga agcgcagttg ctccgggttg cgcggttgga gtctgcgtgc atcggcggcc
     4561 gattcgacca ggtaaatgtc gccgccggcc gcgcgatcgt actgccccat ggtgccttcg
     4621 acctcgggat gaccgcggtg gccgatgagc acggtttcta tgccctggcg gctgtagcgg
     4681 ctgacttcca tgtgcacctt ggtgaccagc gggcaggtgg cgtcgaacac cttcagccgg
     4741 cgccgttcgg cctcgctgcg caccgcctgg gagacgccgt gggcgctgaa gatgacggtc
     4801 gcgccgtcgg ggacctcgtc gagctggtcg acgaagatcg ccccgcgatc gcgcaggccg
     4861 tcgacc
//
LOCUS       DATCZV010000196         3241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_67119, whole genome shotgun sequence.
ACCESSION   DATCZV010000196 DATCZV010000000
VERSION     DATCZV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67119"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VIW27_04430"
     CDS             complement(<1..1245)
                     /locus_tag="VIW27_04430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5738945.1"
                     /translation="MADDAPLIDVRSEQIRIVFAAVPSSLATILAASAILSGVLWSHV
                     DHVAVATWFVLTNLLSLYRLQLYRRFRREDTGRVVDEHWRRPAVLTSMASGLAWGSGG
                     FLLLAEQSPAHQVFLAIVIAGVAAGAVTTMSALLPVVRGFVLLTVTPIIVKFNLVDSE
                     FGVAVTVMSVLFVVLILESAKRLNRMIIESLEVRHQRGLAEQTIRRQAEFDELTDLPN
                     RRLLLSNLRQELAKAQRHQRFGAVFFIDLDRFKAVNDSLGHAVGDELLIKAAQRISER
                     LREEDTVGRLGGDEFVVLLPEIGKTEASSSAHASRIANQIRQLFATPFVVQDHEIHLT
                     ISIGIALFPADVSPEDLLKFADVAMYRAKNEGRDGVRLFSVEMQETVNRQRRVEAGLR
                     HALGNDELQLFLQSQYDSQHRLV"
     gene            complement(1245..2081)
                     /gene="aroE"
                     /locus_tag="VIW27_04435"
     CDS             complement(1245..2081)
                     /gene="aroE"
                     /locus_tag="VIW27_04435"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY5738946.1"
                     /translation="MTAAGAVARYAVVGNPISHSLSPRIHARFAEQTGQALSYQAIEL
                     DVDGFRSGVQDLQQQGYAGLNVTVPFKRDAWEICDRRSRLAEDAGAVNTLSLRPDGGI
                     SGDNTDGTGLVRDLETNLQCVLAGKRILLLGAGGAARGVIGPLLDLTPRSLVIANRTP
                     ARAVELVGIFTGRGEMHAAGFDELGDAHFELVINATAAGLTGELPPLPASCIDTGTIC
                     YDMMYDLAAPTAFVAWARSRGAALACDGLGMLVEQAAEAFFIWRGVRPNSADVIAMLR
                     PH"
     gene            complement(2081..3076)
                     /gene="hemB"
                     /locus_tag="VIW27_04440"
     CDS             complement(2081..3076)
                     /gene="hemB"
                     /locus_tag="VIW27_04440"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY5738947.1"
                     /translation="MASYPDTRKRRMRRDDFSRRLMREHRLTVDDLIYPMFVLDGEQQ
                     REPVESMPDVERVSIDLLLEEAGQLVDLGIPAIALFPVVGAAGKSDGADESWNPDGLI
                     QRAVHELKSRHAELGVITDVALDPYTSHGQDGLLDESGYVTNDATLEALVKQALSHAA
                     AGADVVAPSDMMDGRIGAIRQALEGDGHVNTRILAYAAKYASSFYGPFRDAVGSAGNL
                     GGGNKYTYQMDPANSNEALYEVALDIDEGADMVMIKPGLPYLDIVRRVSEELQFPTFV
                     YQVSGEYAMLKAAGQQGWIDEKACVLEALLSIKRAGANGILTYFSKSVARWLQDS"
BASE COUNT          563 a         1029 c         1077 g          572 t
ORIGIN      
        1 gaccaggcgg tgctggctgt cgtattgcga ctgcaggaac agttggagtt cgtcgttgcc
       61 gagcgcatgc ctgagcccgg cctccacccg ccgctggcgg ttgacggttt cctgcatctc
      121 gaccgagaac aggcgcactc cgtcgcgtcc ctcgttcttg gcgcgataca tcgcgacgtc
      181 ggcgaatttc agtaaatcct ctggcgagac gtcggccgga aacagggcga tgccgatact
      241 gatggtcagg tgaatttcgt gatcctggac gacgaacggg gttgcgaaca gctgccgaat
      301 ttggttggcg atgcgcgatg cgtgagcact gctggaggcc tcggtcttgc cgatctccgg
      361 aagcagcacg acgaactcgt cgccgccgag acgcccaacc gtgtcttcct cgcgcaatcg
      421 ctcggatatc cgctgcgccg ccttgatcag caactcgtca ccgaccgcgt gcccgaggga
      481 gtcgttgacc gccttgaaac gatccaggtc gatgaaaaaa accgcgccga agcgctgatg
      541 acgctgggcc ttggcgagtt cctggcgcag gttcgacagc agtagccgcc ggttcggcag
      601 gtcggtcaac tcgtcgaact cggcctggcg gcggatcgtc tgctcggcga gcccgcgctg
      661 atggcgaact tcgagcgact cgatgatcat tcggttcagg cgcttcgccg attcgagtat
      721 caggaccacg aacagcaccg acatcacggt gacggcaacg ccgaattcgc tgtcgaccag
      781 gttgaacttg acgatgattg gcgtcacggt cagcagcacg aagccgcgta ctaccggtaa
      841 cagcgccgac atcgtggtga ccgcaccggc cgcgacgccc gcgatcacga tggcgaggaa
      901 cacctggtgc gccgggcttt gctcggcgag cagcagaaaa ccgccactgc cccaggcgag
      961 gccggaggcc atgctggtca gcaccgcagg ccgccgccag tgctcgtcga cgacccgtcc
     1021 ggtgtcttcg cgcctgaaac gacgatacag ctgcaggcga tacagcgaca gcaggttggt
     1081 gagcacaaac caggtcgcga ccgcgacgtg atcgacatgg ctccagagca cgccggagag
     1141 gatcgcgctg gccgcgagga tggtcgccag cgaactcggg accgcagcaa agacgatgcg
     1201 aatctgctcc gagcggacgt cgatcagcgg cgcatcgtcc gccatcagtg cggtctgagc
     1261 atggcgatca cgtcggcgct gttcggtcgc acgccgcgcc agatgaagaa ggcttcagca
     1321 gcctgctcga ccagcatgcc gagtccgtcg caggccagcg cggcgccccg cgaccttgcc
     1381 caggcgacga acgcggtggg cgcggcgaga tcgtacatca tgtcgtagca gatcgtaccg
     1441 gtatcgatgc atgatgccgg cagcggcggc agctcgccgg tgagaccggc ggcggtggcg
     1501 ttgatcacca gctcgaaatg ggcgtcaccg agttcgtcga aacccgcggc atgcatctcg
     1561 cctcgcccgg tgaatatccc caccagctcg acagccctgg ccggcgtgcg attggcgatc
     1621 acgaggctgc gtggggtgag atcgagcagc ggcccgatga cgccgcgcgc ggcgccgccg
     1681 gcgccgagca gcaggattcg tttgcccgcg agcacacatt gcaggttggt ctccaggtcg
     1741 cgcaccaggc cggtgccgtc ggtgttgtca ccgctgatac cgccgtcggg ccgcaggctc
     1801 agcgtattga cggcaccggc gtcctcggcc agccggctgc ggcgatcgca gatctcccag
     1861 gcgtcgcgct tgaacggtac cgtgacgttg agccctgcgt aaccctgctg ctgcaggtct
     1921 tgcactccgc tacggaaacc gtcgacgtcc agctcgatcg cctggtaact gagcgcctgg
     1981 ccggtctgct cggcgaaacg cgcgtgaatg cgcggcgaca ggctgtgaga aatcggattg
     2041 ccgactaccg catagcgcgc gaccgctccg gcagccgtca ctagctgtcc tgcagccagc
     2101 gggctacgga tttggaaaag taggtcagaa tgccgttggc gccggcgcgc ttgatcgaca
     2161 gcagcgcctc gagtacgcaa gccttttcgt cgatccagcc ttgctggccg gcggccttga
     2221 gcatcgcata ctcaccgctg acctggtaga cgaaagtcgg gaactgcagt tcctcgctga
     2281 cccggcgcac gatgtcgagg tagggcaggc cgggcttgat catgaccatg tcggcgccct
     2341 cgtcgatatc gagcgcgacc tcgtagagcg cctcgttgga attcgccggg tccatctgat
     2401 aggtgtactt gttgccgccg cccagattgc cggcggagcc gaccgcgtcg cggaatggcc
     2461 cgtaaaagct cgacgcatac ttggccgcat aggcgaggat gcgggtgttg acatggccgt
     2521 cgccttcgag cgcctggcga atcgcgccga tgcggccgtc catcatgtcc gatggcgcga
     2581 ccacgtcggc accggccgcg gcatgcgaca gtgcctgctt gaccagtgcc tccagggtgg
     2641 cgtcgttggt cacgtagccg gactcgtcga gcagaccgtc ctggccgtga ctggtgtagg
     2701 ggtcgagcgc gacatcggtg atgacgccga gttccgcatg gcggctcttc agttcgtgca
     2761 cggcgcgctg gatcaggccg tcgggattcc agctttcatc ggcgccgtcg ctcttgccgg
     2821 cggcgccgac caccgggaac agcgcgatcg ccggaatccc gagatcgacg agctgaccgg
     2881 cttcctcgag taacaggtcg atgctgacac gctcgacgtc gggcatggat tcgaccggct
     2941 cgcgctgctg ctcgccatcg agaacgaaca tcgggtagat caggtcgtcc acggtcaggc
     3001 gatgctcgcg catcaggcgc cgtgaaaagt cgtcgcgccg catgcggcgc ttgcgggtat
     3061 cgggatagct ggccacgcct tgctcggatt gttcgggggt gcctaatatc gcataaatct
     3121 gatctcaggg acaggcgttg gcggcccgtg actggcgctg cgcggcgtcg gcggcatgcg
     3181 gggaagcttc gcccgctttg ttcaattgtt tgccgattgc ggcattccgg actataagct
     3241 t
//
LOCUS       DATCZV010000197         3473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_650412, whole genome shotgun sequence.
ACCESSION   DATCZV010000197 DATCZV010000000
VERSION     DATCZV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650412"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VIW27_04445"
     CDS             complement(<1..706)
                     /locus_tag="VIW27_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HEY5738948.1"
                     /translation="MRFKSSSPISLAVLLTLLAAALLYRFAVPAGLDNGFDLSNSILP
                     RNEIQHGGPPRDGIPALSDPRLVTAGEADYLQPADRVLGIEIDGEARAYPIPILNWHE
                     IVNDTVGGRRLAITYCPLCGTGVAFDAHIDGEPTDFGVSGLLYNSDVLLYDRASESLW
                     SQILGKAVSGERVGKRLLPVPLTHTTWKAWRASYPHTLVLSDDTGYSRNYQRDPYDGY
                     EQSRHTYFSVNNAAPAS"
     gene            868..1983
                     /locus_tag="VIW27_04450"
     CDS             868..1983
                     /locus_tag="VIW27_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY5738949.1"
                     /translation="MKARAAVAWEPKKPLEIEEVEVEGPREGEVLLRVHATGVCHTDA
                     FTLSGEDPEGAFPAILGHEGGCEVLECGAGVSSLQPGDHVIPLYIPECQQPACPYCSS
                     NKNNLCQSIAGTVWTGFMPDGTRRFRARGKEIYHYMGCSTFAEYTVVPEIALAKISPQ
                     APLDMACLLGCGVTTGIGAVLNTAKVEPGSSVAVFGLGTVGLSVIQGAVMAKAERILA
                     IDINPGKEEIARQLGATDFVNSAEIDGSIPEYIQEMTGGGVDYSFDCTGIVQVMRDAL
                     ECTHMGWGTSCIIGVAGAGEEIRTRPFNLVVGRRWIGTAFGGVKGRTELPGMVDQYMN
                     GQIELDKLVTHTMPLEEINTAFDLMHEGKSIKSVIKF"
     gene            1992..2831
                     /gene="fghA"
                     /locus_tag="VIW27_04455"
     CDS             1992..2831
                     /gene="fghA"
                     /locus_tag="VIW27_04455"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY5738950.1"
                     /translation="MKEIESIKESGGYLNRYTHASAVCNCEMTFSVYLPPQSRNARVP
                     ALYWLSGLTCSDDNARTKGGFQRFAARHGVAIVFADTSPRGDDVADQVERYDLGQGAG
                     FYVNATQDPWRPHYQMYAYVTAELPALIEAELPLQAGVKSITGHSMGGHGALVCALRQ
                     PNAYRSVSAFAPISNPVACPWGEGCFGAYLGDDRGAWEAYDATCLLRAGARVGDILVD
                     QGDADEFLHEGQLLPEHLQAACDAAGQPLTLRMQPGYDHSYHFIASFIEDHFEYHAGF
                     LNG"
     gene            3023..>3473
                     /locus_tag="VIW27_04460"
     CDS             3023..>3473
                     /locus_tag="VIW27_04460"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008758606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEY5738951.1"
                     /translation="MSDFDADKVAKIHRELHSHLPSEPALRVKALETLLVDKGLVNSQ
                     TIDAWVEAYTEEIGPKRGARVVAKAWTDPEYRKRLLADAPAAIDELGYLGKATAHLKA
                     VENTDTVHNLVVCTLCSCYPFSILGIAPAWYKAAAYRARAVRDPRGVL"
BASE COUNT          647 a         1086 c         1137 g          603 t
ORIGIN      
        1 aactggccgg cgccgcatta ttgaccgaaa aataggtgtg gcgggactgt tcgtagccgt
       61 cgtacggatc gcgctggtag ttgcgcgaat aacccgtgtc gtccgacagc accagggtat
      121 gcgggtagct ggcccgccag gccttccagg tggtgtgggt gagcgggacc gggagcaggc
      181 gcttgccgac gcgctcgccg gacaccgcct tgccgaggat ttgtgaccac agcgactcgc
      241 tggcgcggtc gtaaagcaac acgtcgctgt tgtacagcag gccggagacg ccgaagtcgg
      301 tcggctcacc gtcaatatgg gcgtcgaagg cgaccccggt gccgcacagc gggcagtagg
      361 taattgccag gcggcggccg ccgaccgtat cgttgacgat ttcgtgccag ttcaggatcg
      421 ggatcgggta ggcgcgggct tcaccgtcga tctcgatgcc gagcacgcgg tccgcgggtt
      481 gcaggtaatc ggcttcgccg gcggtgacca gtcgcgggtc ggacagcgcc ggtatgccat
      541 cccgcggcgg cccgccatgc tggatttcgt tgcgtggcag gatcgagttc gacagatcga
      601 agccgttatc gagtcccgcc gggaccgcaa agcggtacag caacgcggcg gcgagcagcg
      661 tcagcaatac cgctaatgaa attgggctgg aggatttgaa tcgcatttcg ggctcccctg
      721 cagttgacct cgataaggtt tgagtcccga atcggcaaat aattcaatcg ctttgcgctc
      781 gccgccaggt gtcgtggcca gcttgactcg atggttgaat ctcaattagt ctgcggcaac
      841 tcaaaaaaac tatccgcagg agagtccatg aaagccagag ccgcggttgc gtgggaaccg
      901 aaaaaacccc tcgagatcga ggaagtcgaa gtcgaaggcc cgcgcgaggg cgaggttctg
      961 ctgcgggtgc atgcgaccgg cgtctgtcat accgacgcgt tcacgctttc cggcgaggat
     1021 cccgagggcg cctttccggc gattctcggc cacgagggcg gctgcgaagt gctcgagtgc
     1081 ggtgcgggcg tcagcagcct gcagccgggc gaccacgtca tcccgctata tattcccgaa
     1141 tgccagcaac cggcctgtcc gtactgcagt tccaacaaga ataacctttg ccagtcgatc
     1201 gctggcaccg tgtggaccgg gttcatgccc gacggtacgc gccgtttcag ggcccgcggc
     1261 aaggaaatct accactacat gggctgttcg acgtttgccg agtacaccgt ggtgccggag
     1321 atcgcgctcg ccaagatcag cccgcaggcg ccactcgaca tggcctgcct gctcggctgc
     1381 ggcgtcacca ccggcatcgg cgcggtattg aataccgcga aggtcgagcc cggctcgtcg
     1441 gtcgcggttt tcggtctcgg cacggtcggt ctgtcggtca ttcaaggggc ggtgatggca
     1501 aaggcggagc ggattctggc catcgacatc aacccgggca aggaagaaat cgcccgtcag
     1561 ctgggagcca ccgatttcgt caattcggcg gagatcgacg gctcgattcc ggaatacatc
     1621 caggagatga ccggcggcgg tgtcgactat tccttcgact gtaccggcat cgtgcaggtg
     1681 atgcgcgatg cgctcgagtg cacgcacatg ggttggggca cgtcgtgcat catcggcgtc
     1741 gccggtgccg gcgaggaaat ccgcacgcgc ccgttcaacc tggtcgtcgg ccggcgctgg
     1801 atcggcaccg cattcggcgg cgtcaagggc cgcaccgagc tgcccgggat ggtggatcaa
     1861 tacatgaacg gtcagatcga gctcgacaag ctggtcacgc acaccatgcc gctggaggaa
     1921 atcaacaccg ctttcgacct gatgcacgaa ggcaagagca tcaagtcggt aatcaagttc
     1981 tgagccgcgc gatgaaggaa atcgaaagca tcaaggaaag cggcggctat ctcaaccgct
     2041 atacgcatgc gtcggcggtc tgcaactgcg agatgacctt ttcggtctac ctgccgccgc
     2101 aatcgaggaa tgcgcgggtg ccggcgctct actggctgtc gggcctgacc tgcagcgacg
     2161 acaatgcccg caccaagggc ggcttccagc gcttcgcggc ccggcatggc gtcgctatcg
     2221 tgttcgcgga caccagcccg cgcggtgacg acgtcgccga ccaggtggaa cgctacgacc
     2281 ttggccaggg tgccggcttt tacgtcaacg cgacgcagga tccgtggcgc ccgcattatc
     2341 aaatgtacgc ttacgtgacc gccgaactgc cggcgctgat cgaggccgag ttaccgttgc
     2401 aggccggggt caagtcgatc accggtcatt cgatgggcgg acacggcgcg ctggtctgcg
     2461 cgctcaggca gccgaatgcg tatcgctcgg tatcggcgtt cgcaccgatc agcaacccgg
     2521 tcgcctgtcc ctggggagaa ggctgtttcg gtgcctacct gggcgacgac cgcggcgcct
     2581 gggaggctta cgacgccacc tgcctgctgc gagccggcgc gcgtgtcggc gacatcctgg
     2641 tcgaccaggg cgacgccgac gaattcctgc acgaggggca attattgccg gaacacttgc
     2701 aggcggcttg cgacgcggcg ggccagccgc tgacgctgcg catgcagccg ggctacgacc
     2761 acagctatca tttcatcgcc agcttcatcg aagaccattt cgagtaccac gccggattcc
     2821 tcaacggcta agcgatcggc cgtgccgtgc ggcccacggg aagtcgaaca cttcaccggc
     2881 tgtggccacc cggtgtctac tcgcgcgggc gccggttagc tgacgaatgc gccgaatgcc
     2941 acgcggctga atcggcaatc ggcgccgccg gagtcgtgcc gcggcgctta aatttcggcg
     3001 ccgaatacag taagctcgct gcatgagcga tttcgacgcc gacaaggtag ccaaaataca
     3061 ccgcgaactg cacagccacc tgccgtcgga accggcgttg cgcgtcaagg cgctggaaac
     3121 cctgctggtc gacaaggggc tggtcaattc gcagaccatc gacgcctggg tggaagccta
     3181 taccgaggag atcggcccga aacgcggcgc ccgcgtagta gccaaagcct ggaccgatcc
     3241 cgagtaccgc aagcggctgc tcgccgacgc gcccgcggcg atcgacgagc tcggctacct
     3301 cggcaaggcc accgcccacc tcaaggcggt cgagaatacc gacacggtgc acaacctcgt
     3361 ggtctgcacg ctgtgctcgt gctacccgtt ttcgatcctc ggtatcgcgc cggcctggta
     3421 caaggccgcg gcctaccgcg cgcgcgcggt gcgcgatccg cgcggcgtac tcg
//
LOCUS       DATCZV010000198         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_650428, whole genome shotgun sequence.
ACCESSION   DATCZV010000198 DATCZV010000000
VERSION     DATCZV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650428"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VIW27_04465"
     CDS             <1..555
                     /locus_tag="VIW27_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY5738952.1"
                     /translation="IPDDVVLDNLVFTTDAKGERSKVGELMKITMTPEYAEQAFGIVP
                     PDLSLIARSRGVDWLYNYLRGFYVDETREGLGVNNGVFANVGMPHVLAELQGLQAPKY
                     RTVTDAEGNEQQIINGFEIVEPGSMSPSDYDRMIHDLVAFLDYLAEPYKQTRKNVGTG
                     VIIFLFFFLILAYLLKKEYWKDVH"
     gene            572..1198
                     /locus_tag="VIW27_04470"
     CDS             572..1198
                     /locus_tag="VIW27_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5738953.1"
                     /translation="MSIVANRRSVMSCFSSPTDPECHRARIVLAEKDITVEIHDVDTN
                     NLPEDLLDLNPYATVPTMVDRDLVLYNARVLIEYLDERFPHPPLMPVDPVSRAKSRLA
                     LFRIESDWFSLLPDIERGTEKRKAQAKKALAESLINSAEVFGAMPYFLSHEFSLLDCS
                     IAPILWRLPYYGIELPSSAKQVTAYMKRVFSRPSFQASLSEQERDMVA"
     gene            1209..1586
                     /locus_tag="VIW27_04475"
     CDS             1209..1586
                     /locus_tag="VIW27_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY5738954.1"
                     /translation="MTSSKPYLVRALCQWIVDNGKTPHILIDTTLKGVVLPPGIGSDG
                     SAVLNLAPQAVQKLEMTNEHVAFSARFNGVVHDVYCPIESLLGIYARETGKGMMFPPE
                     EDVGDDGTGDDDKPRGPSLTVVK"
     gene            complement(1750..2115)
                     /locus_tag="VIW27_04480"
     CDS             complement(1750..2115)
                     /locus_tag="VIW27_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY5738955.1"
                     /translation="MSWRRKGLRFEDRARSYLERRGLSLLSQNFHGRRGEIDLIMRDG
                     DRICFIEVKYRKSRAYGGAANAIPVAKRRRIVDTALYYLSTRATAARLAPRFDAVLIQ
                     QQPDGGEAIEWIRNAFYAE"
     gene            complement(2124..>2354)
                     /locus_tag="VIW27_04485"
     CDS             complement(2124..>2354)
                     /locus_tag="VIW27_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY5738956.1"
                     /translation="RLGPLFALGIDAYRVIPQLRRLLINPDESLAHNTGVLSVGTDGR
                     VRRSLRMATYENGRARPMQTPLLREQTSSLQP"
BASE COUNT          468 a          699 c          720 g          467 t
ORIGIN      
        1 attccagacg acgtggtgct cgacaacctg gtgtttacca ccgacgccaa aggcgaacgc
       61 agcaaggtcg gtgagctgat gaagatcacc atgacccccg aatacgccga gcaggcgttt
      121 ggtatcgtgc cgccggacct gtcgctgata gcgcgctcgc gcggggtcga ctggctgtac
      181 aattacctgc gcggatttta cgtcgacgag acgcgcgaag gcctgggcgt caacaacggt
      241 gtgttcgcca atgtcggcat gccccacgta ctggccgaac tgcagggcct gcaggcgccc
      301 aagtacagga cggtgaccga tgccgagggt aacgaacaac agattatcaa cggtttcgaa
      361 atcgtcgagc cgggttcgat gtcgccgtcc gattacgatc gcatgattca cgacctggtg
      421 gctttcctcg actatctcgc ggaaccctac aagcagacgc gtaagaatgt cggtaccggt
      481 gttattattt tcctcttctt cttcctgatt cttgcttact tgttgaagaa agaatactgg
      541 aaggatgtcc actaactaag gattagaaat tatgtctatc gtggcgaatc gtcgctcggt
      601 gatgagttgt ttttcctctc cgaccgatcc cgaatgccac cgcgcgcgga tcgtgctcgc
      661 ggaaaaggac atcacggttg aaatccacga tgtggatacc aataacctgc ccgaggattt
      721 gctcgatctg aatccctacg ccaccgtgcc gacgatggtc gaccgcgacc tggtgctgta
      781 caacgcgcgc gtgctgatcg agtatctcga cgagcgtttc ccgcatccgc cgttgatgcc
      841 ggtggacccg gtatcccgcg ccaagagccg gctggccctg tttcgcatcg agagcgactg
      901 gttcagcctg ttgcccgata tcgagcgcgg taccgaaaag cgcaaggcgc aggcgaaaaa
      961 ggcgctcgcc gaaagcctga tcaattcggc cgaggtattc ggcgccatgc cgtatttcct
     1021 gtcgcatgaa ttcagcctgc tggattgcag cattgcgccg atcctgtggc ggctgcctta
     1081 ttacgggatc gaactgccga gttcggcaaa gcaggtcacc gcctacatga agcgggtgtt
     1141 ttcgcgcccg tcgttccagg ccagcctctc cgagcaagag cgggacatgg tcgcctgatc
     1201 acccggcgat gacctccagc aagccctacc tggttcgcgc gctctgtcaa tggatcgtcg
     1261 ataatggcaa aacgccgcat atcctgatcg ataccacgct aaagggtgtg gttctgccgc
     1321 ccgggatcgg cagcgacggc agcgcggtgt tgaacctggc gccgcaggcg gtgcagaaac
     1381 tcgaaatgac caatgaacac gtagcgtttt cggcgcgctt caacggtgtc gtccatgacg
     1441 tctattgccc catcgaatcg ctgctgggca tctacgcgcg tgaaaccggc aaggggatga
     1501 tgtttccgcc cgaggaagac gtcggcgacg acggtaccgg ggatgacgac aagcccaggg
     1561 ggccgagtct cacggtcgtc aaatgatcct gccagctgtg atcgggtaac gattgcagcc
     1621 aggcagcttg ccggcggctc gcgcaccaac gcctgcagcc cggtcgaagc cgaggcagcg
     1681 gcaatccggc gttgatatcg acgatttcag gttcggcgcg agcccgcaga tgaccagcgg
     1741 ctgctgcagc tattccgcgt aaaacgcgtt cctgatccac tcgatcgcct caccgccatc
     1801 gggctgctgc tggatcagca ccgcgtcgaa acgcggtgcc aggcgggcgg cggtcgcacg
     1861 cgtggacagg taatatagcg cggtgtcgac aatgcggcgt cgtttggcga ccgggatcgc
     1921 gttggcggca ccgccgtagg cgcgggattt tcgatacttg acctcgatga agcagatgcg
     1981 gtcaccatcg cgcatgatca ggtcgatttc gccgcggcgg ccatgaaagt tctgcgacag
     2041 cagcgacagg ccgcgccgtt cgagataaga ccttgcccgg tcttcgaatc gcaatccttt
     2101 gcgccgccag ctcatttacc gtttcagggt tgtagcgaag acgtctgctc gcgaagcaac
     2161 ggcgtttgca tcggccgagc gcggccgttt tcgtaggtcg ccatccgcag cgaacgcctt
     2221 accctgccgt cggtgccgac cgacaggacg ccggtgttgt gcgccagcga ttcgtcgggg
     2281 ttgatcagca ggcggcgcag ctgcgggatc acccggtagg catcgatacc gagcgcgaac
     2341 aacgggccga gacg
//
LOCUS       DATCZV010000199         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_491096, whole genome shotgun sequence.
ACCESSION   DATCZV010000199 DATCZV010000000
VERSION     DATCZV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491096"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1311)
                     /locus_tag="VIW27_04490"
     CDS             complement(184..1311)
                     /locus_tag="VIW27_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY5738957.1"
                     /translation="MRKSRLLFFAALLLPLTASADDAAAPESRLSGLLSGDGDFGFEK
                     AIEPRAFVFPEDHGPHPEFRNEWWYVTGNLDGENGRRFGFELTIFRFALTPGLTATES
                     NWRSNQVYIAHFAVTDAGSERFYAAERYSRGALGLAGASADPFRVWIDDWEIAAAENA
                     SPEQWRLRAADPEFALDVALTAAKPPVLNGVDGLSQKSAEPGNASYYYSITRWLTEGS
                     LRLGDDEYRVSGLSWLDREWSSSALGADQLGWDWFALQLSDGSELMFYNLRKLDGSQD
                     GHSAGTWINADGTSTHIDREDIEIVVSDTWDSPEGGRYPSAWTLKLPQQGLSLEIRPV
                     MADQELFTTVRYWEGAVDIEGERGGSPVEGRGYVELTGYAE"
     gene            complement(1321..>2587)
                     /locus_tag="VIW27_04495"
     CDS             complement(1321..>2587)
                     /locus_tag="VIW27_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5738958.1"
                     /translation="ILSGRNLVAGLIAMFLLIFGFALCVPLAVKVASAVLAPLASRIG
                     GMLARMAVSGIGESLSRTGVAIVALAVAVSATIGVSVMVDSFRGSVDEWLQRTLQADV
                     YAGVQRGSLDPGLLGDIVSLDGVGSSSTSRRVQLEDAAGRTQLIVIRMAPGGYAGTEI
                     IDANPDDVWPAWERDDVVLVSEPYAYQHEVSRGDIVQLPTDRGPHSFVIAAIYQSYDI
                     NASAMLMSRVVYDRHFDDDGVDSVGLYLSGGVDPEAIMARIGEISEGRQEIRFNSNAR
                     IRELSLEIFDRTFIITDVLYWLAVGVAFIGILGAMLALQLERGRELAVLRALGMTPWQ
                     LGGLITTQTAMIGLLSGVAAVPLGIMMAYVLIEVINRRAFGWQIDMSVAPDILLSAIA
                     FAVGAALLAGIYPSFRAARSQPAAAMREE"
BASE COUNT          510 a          870 c          791 g          416 t
ORIGIN      
        1 acgagcaatc attgccgggc gcactcgccg accgcgcggc gctggaagac gatacggtcg
       61 cctaccgctg cctcggcacg cattgcgagc tgcctgtgac gagctgggag gcgctcgccg
      121 ccagcctgtc ggaaaccgcc tgatatcgat gcgctaccgc aggagcggtt ccggaaccgc
      181 gacctattcg gcgtaacccg tcagttcgac gtagccgcga ccctcgacgg gcgaaccgcc
      241 gcgttcgcct tcgatatcta cggcgccttc ccagtagcgc accgtcgtga agagctcctg
      301 gtcggccatg acagggcgga tctccagcga gagtccctgc tgcggtaatt tcagcgtcca
      361 ggccgacgga tatcgtccac cctcgggact gtcccaggta tcgctgacca cgatctcgat
      421 gtcctcccgg tcgatgtgcg tcgacgtgcc gtcggcgttt atccaggtgc cggcactgtg
      481 cccgtcctgg ctaccgtcga gcttgcggag gttgtagaac atcagctcgc tgccatcgga
      541 cagctgcagc gcgaaccagt cccagccgag ctgatccgcg ccgagcgcgc tgctgctcca
      601 ctcgcgatcg agccaggaca agcccgaaac ccggtactcg tcatcgccga ggcgcagcga
      661 accctccgtc agccatcggg tgatggaata gtaataggag gcgttgcccg gctcggccga
      721 cttctgcgaa agcccatcga cgccgttcag cacgggtggt tttgcggcag tgagcgcgac
      781 gtcgagcgcg aactcagggt cggccgcccg caatcgccat tgctcggggg acgcgttttc
      841 cgctgccgct atttcccagt cgtcgatcca gacgcgaaat ggatcagcac tcgcgcccgc
      901 gagcccgagc gcgccccgcg agtaacgctc ggccgcatag aagcgctcgc tgccggcatc
      961 ggtcacggca aagtgcgcaa tgtagacctg gttactgcgc cagttcgatt ccgtagccgt
     1021 gaggcccggc gtcaacgcga agcgaaagat cgtcagctcg aagccgaagc gcctgccgtt
     1081 ctcgccgtcg aggttgccgg tgacgtacca ccactcgttg cgaaattcgg gatgcgggcc
     1141 atggtcttcg ggaaatacga acgcgcgtgg ttcgatggct ttctcgaacc cgaaatcgcc
     1201 atcgccgctc aacaacccgg acagccgcga ctcgggcgcc gcagcatcgt cggcagacgc
     1261 cgttaacggc aacagcaggg ctgcaaaaaa aagcaggcgg ctcttgcgca ttgcggccac
     1321 ctactcctcc cgcatggccg cggccggctg gctccgggcg gcacgaaagg aaggatagat
     1381 gccggccagc agcgcggcgc caacagcgaa agcaattgca gacaagagga tatcgggggc
     1441 gaccgacatg tcgatctgcc agccgaacgc acggcggttg atgacctcga tcagcacgta
     1501 agccatcatg atccccagcg gcaccgccgc gacgccgctg agaaggccga tcatcgcggt
     1561 ctgggtcgtg atcagtccac caagctgcca cggcgtcatg ccgagcgcgc gcaataccgc
     1621 gagctcacgg ccgcgctcca gctgcagcgc cagcatggcg ccgaggatgc cgatgaacgc
     1681 cacgccgacc gcgagccagt aaagcacgtc ggtaatgatg aacgtacgat cgaagatctc
     1741 gagggagagc tcacggatgc gcgcattgga attgaatcgg atctcctgcc ggccctcgct
     1801 gatctcgcca atgcgcgcca taatcgcctc cggatccaca ccgccggaca gatacaggcc
     1861 tacggaatcc acgccatcat cgtcgaagtg ccggtcgtag acgacgcggc tcatgagcat
     1921 cgcgctcgcg ttgatgtcgt agctctggta gatggctgca atcacgaaag agtgtggccc
     1981 gcggtcggtc ggcagctgca cgatgtcgcc acgcgaaacc tcgtgttgat aggcataggg
     2041 ctcggatacc agcacgacgt cgtcacgttc ccaggccggc catacgtcgt cgggattcgc
     2101 atcgatgatc tcggtgccgg catacccgcc cggcgccatc ctgatcacga tcaactgcgt
     2161 gcggccggcg gcatcttcca gctgtacgcg ccgactggtg ctggaagacc cgacgccgtc
     2221 gagcgaaacg atatcgccga gtaatcccgg atccagtgat ccccgctgca cgcccgcgta
     2281 gacgtcagct tgcagtgttc gctgcagcca ttcgtcgacg gaaccgcgga aactgtccac
     2341 cattacgctc acaccgatcg ttgcggaaac cgctaccgcc agcgcaacga tcgcaacgcc
     2401 cgtgcggctc aggctttcgc caatcccgga taccgccatg cgcgccagca tgccgccgat
     2461 tcgcgatgcc aaaggcgcca gcaccgccga cgcgaccttg accgcaagcg gcacgcaaag
     2521 cgcgaaaccg aagatcagca ggaacatcgc gatgagtccc gcgacgagat tgcggccgga
     2581 aagaatc
//
LOCUS       DATCZV010000200         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_174129, whole genome shotgun sequence.
ACCESSION   DATCZV010000200 DATCZV010000000
VERSION     DATCZV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174129"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..586)
                     /locus_tag="VIW27_04500"
     CDS             complement(8..586)
                     /locus_tag="VIW27_04500"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY5738959.1"
                     /translation="MDDEKQRCWWCGEDPLYVAYHDREWGVPVHGERELFEFLCLEGA
                     QAGLSWITILRKRDNYRRAFDNFDAQRIACYGDDRIQALLQDSGIVRNRLKVNGFVRN
                     ARAYLELLDEGMTLDEYLWGFVDGETIHNRRESRAEVPATSAESDTMAKALKKRGFTF
                     VGSTICYAFMQAAGLVNDHLVDCYRHRELIGG"
     gene            complement(587..1756)
                     /locus_tag="VIW27_04505"
     CDS             complement(587..1756)
                     /locus_tag="VIW27_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5738960.1"
                     /translation="MQFSSLTERVKGGAADAWDLHYAAKLAHERGEDVIVLSVGDPDF
                     ATPPGIVERAFAAIAEGDTHYTSIAGHRGLREAIAARHRALSGQLVDADQVITTSGAQ
                     NALFSCALCLLDHGDEAIVLQPMYVTYEASVQAPGAILVPVVLDADNGFRLRREALQA
                     AVSERTRAIFYASPSNPTGIALNAEELDFIAELAVQHDLWVVADEVYGDFVFDGEFRP
                     AASHPGFDDRLVTIGSLSKSYAMSGWRVGWAIGPPQLIANMDKLALCMLYGLPGFIQQ
                     AAQYALENCAAERDRMRDIYRDRLRRMHAAFAAMPGLAPLMPEAGMFMLVDVRGTGLA
                     VSEFTRRLYDSTGVAVLDATAFGASAAGHVRVSATVGDAELDAAISRIRRFVEGL"
     gene            complement(1761..2336)
                     /locus_tag="VIW27_04510"
     CDS             complement(1761..2336)
                     /locus_tag="VIW27_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006991408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY5738961.1"
                     /translation="MQKRFVEEQELLEDAYRLAVGVFDSGFRPDFIVGLWRGGSTVGI
                     YVQECLQYLGVETDHIAIRTSYRGREDYLRRLEEHGEIRAHGLQYLFDHLNADNRLLL
                     VDDVYSTGRNIDAVITRLRRTLRRNTPQEIRIAAPWYRPGGGRAPDYYLHETADWLVL
                     PYELSGLTRAEAMAHKPWVVPLLDELRPGWT"
BASE COUNT          458 a          823 c          769 g          433 t
ORIGIN      
        1 gacatcttca acctccgatc agttcgcggt ggcggtagca atcgaccagg tgatcgttga
       61 ccaggcccgc cgcctgcatg aacgcataac atatggtact gccgacgaat gtaaagcccc
      121 gctttttcag cgccttcgcc atcgtgtcgg actcggcgct cgtcgccggc acctcagccc
      181 tcgattcgcg ccgattgtgg atggtttcac cgtcgacgaa tccccacagg tattcatcca
      241 gcgtcatgcc ctcgtcgagc aactcgagat aggcgcgcgc attcctgaca aaaccgttga
      301 ccttgagccg gttgcgcacg atacccgaat cctgcagcag cgcctggatt cgatcatcgc
      361 cgtaacacgc gatccgctgc gcatcgaaat tgtcgaatgc gcggcggtaa ttgtcgcgct
      421 tgcgcagaat cgtgatccac gacaatcccg cctgtgcgcc ctccagacac aggaattcga
      481 acagctcgcg ctcgccgtgc accggcaccc cccattcccg gtcgtggtag gcgacgtaca
      541 gcgggtcttc gccgcaccac cagcagcgtt gtttttcatc atccatctac aggccctcga
      601 cgaagcggcg aatgcggctg atcgcagcgt cgagctcggc atcgccgacc gtcgccgaga
      661 cccgcacgtg gccggccgcg ctggcgccga aagcggtcgc atcgagcacc gctacgccgg
      721 tgctgtcgta gagtcgccgg gtaaattcgg acaccgccag accggtgccg cgcacgtcga
      781 cgagcatgaa catgccagcc tcgggcatca gcggtgcgag acccggcatc gcagcgaaag
      841 ccgcatgcat gcgccgcagc cggtcacggt agatgtcgcg catgcggtca cgctcggcgg
      901 cgcagttctc gagcgcgtat tgcgcggctt gctgaatgaa gcccggcaat ccgtagagca
      961 tgcacagcgc gagcttgtcc atgttggcga tcaattgcgg cggaccgatc gcccagccga
     1021 cgcgccagcc ggacatcgcg taggatttcg acaggctgcc gatggtcacc aggcggtcgt
     1081 cgaacccggg gtgcgaggcc gccgggcgaa actcgccgtc aaacacgaaa tccccgtaga
     1141 cctcgtcggc gaccacccac aggtcgtgct gcacggcgag ttcggcgatg aaatcgagct
     1201 cctcggcgtt gagcgcgatg ccggtcgggt tgctcggcga ggcgtagaaa atcgcgcggg
     1261 tgcgctcgct gacggcggcc tgcagcgctt cgcgacgcag tcgaaagcca ttgtcggcat
     1321 cgagcaccac cggcaccagg atcgctcccg gcgcctgcac gctagcctcg taggtgacgt
     1381 acatcggctg cagcacgata gcttcgtcgc catgatcgag caggcacagc gcgcacgaaa
     1441 acagtgcatt ctgcgcgccg gaagtcgtga ttacctggtc ggcgtcgacc agctggccgg
     1501 aaagcgcgcg gtgacgagcg gcaatcgctt cgcgcagccc gcgatgcccg gcgatcgacg
     1561 tgtaatgagt atcgccctcg gcgatcgccg caaacgcgcg ctcgacgatg cctggcggcg
     1621 tggcgaagtc aggatcgccg accgacagca cgatgacgtc ttcgccgcgc tcatgagcca
     1681 gcttggcggc ataatgaagg tcccaggcgt cggccgcgcc gcccttgacc cgctcggtca
     1741 gcgaggaaaa ttgcatcggc ttacgtccac ccgggcctga gttcgtcgag cagcggcacc
     1801 acccagggtt tgtgcgccat cgcctcggct cgcgtcaggc cgctcaactc gtagggtagt
     1861 accagccagt ccgcagtctc gtgcaggtaa taatcgggcg cccgcccgcc gcccgggcga
     1921 taccagggcg ccgcaatgcg tatctcctgc ggcgtgttgc gccgcagggt gcggcgcagg
     1981 cgggtgatca ccgcgtcgat gttgcgcccg gtgctgtaga cgtcgtcgac cagcagcagg
     2041 cgattgtcgg cgttcaggtg gtcgaacagg tattgcaggc cgtgagcgcg aatctcgccg
     2101 tgttcttcga gacgccgcag gtaatcctcg cggccgcgat acgaagtgcg aatcgcgatg
     2161 tggtcggttt cgacgccgag atattgcagg cattcctgca cgtagatacc gaccgtcgac
     2221 ccgccgcgcc acaggccgac gatgaagtcg ggtcgaaaac cgctgtcgaa gacgccgacc
     2281 gccaggcgat aggcatcctc gagcaactcc tgttcctcga cgaaccgttt ttgcatgctg
     2341 aacgcgccgt ttcccaagct gctgtgctat tctgccgcag cctaccatag accccggtaa
     2401 atgcatcccc tcgatgagcg aaaaacgcta cctatcggcg cagcagttgc tcgaagattc
     2461 ttttcggctc ggcgcgcgca tca
//
LOCUS       DATCZV010000201         4136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_545077, whole genome shotgun sequence.
ACCESSION   DATCZV010000201 DATCZV010000000
VERSION     DATCZV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545077"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VIW27_04515"
     CDS             <1..498
                     /locus_tag="VIW27_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5738962.1"
                     /translation="MIARLMPLMEKGEVANASKLARESNATVSRILLQGMEHYKSARR
                     RDDFEAVMDESVMEAIPRLEKRVHYLAMFANMATLLGLLGTIVGLIKAFTAVAQVDPS
                     MKAEILSTSISVAMNTTAFGLIVAIPLLLFYTMLQTKATEIVDNLETTVVKFVNLLWR
                     VKSRQ"
     gene            507..1010
                     /locus_tag="VIW27_04520"
     CDS             507..1010
                     /locus_tag="VIW27_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5738963.1"
                     /translation="MSKRTARMRHPAAELNITAFMNLMVVLVPFLLMTAVFTQITVLD
                     LKLPPPDALNKPQPEQKPYELRLTIRADSLVLSDNRVGLIKQFARRDGKHDFAQLKSA
                     LKQVKARIPAHTGITILSEQQTRYKDLIEAMDSSRSYRTQVEGEWVFAELFPNISIGD
                     AARQVAK"
     gene            1007..1495
                     /locus_tag="VIW27_04525"
     CDS             1007..1495
                     /locus_tag="VIW27_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5738964.1"
                     /translation="MSTRRDKRKQKFKARRRPRATLLNLVSLMDIFTILVFFLLVNAS
                     TAEVLPSPKNILLPEAGADKLPALNLVIAVDDRVIRLQGKQVIAVSDVLEDGKSSIRP
                     LYDALLEARKTVKNLTDKQGVTIMGDQEIPYALLKKIMLTCTGAEYTNISFAVIQKVA
                     ES"
     gene            1495..2442
                     /locus_tag="VIW27_04530"
     CDS             1495..2442
                     /locus_tag="VIW27_04530"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY5738965.1"
                     /translation="MSATATARAGDMLWMDHQEDRGFRWLSIFLLVLFLTGGVVLNSI
                     ELPEVEQKRLVDVSPRLAKLILEKQKEPPPPPPAPKPKKEQAKKEKPAEKKKPPPKKA
                     EKAPPKKSAREVAQQSGLIAMADELADLRDSFALDDLAALPQTSAGKQAIEVASTDQL
                     LTAGAGRSSGGIQTDTLNRKITTSELAQRRTTSVESAIEAAAGKSVASSSSATRQKDL
                     STVRSRDEIERVFQQYKGGIFNIYNRALRKDPTLAGKVVIELTIAPDGSVKAARILSS
                     ELGDEQLERKLILKIKRFKFSKSNVAEITVTYPIEFLPS"
     gene            2488..2685
                     /locus_tag="VIW27_04535"
     CDS             2488..2685
                     /locus_tag="VIW27_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738966.1"
                     /translation="MRLGGSRAALSDVRNQRSGQPFGRCHQVGENLKLGVGHDFTDFA
                     DGSPDLDFDNQDFFGNVTSKL"
     gene            2930..3520
                     /locus_tag="VIW27_04540"
     CDS             2930..3520
                     /locus_tag="VIW27_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5738967.1"
                     /translation="MSAPFAIATSRLRLRWLTASDADFVLRLVNDAEWLRFIGDKGAP
                     DLDGARRYIEAGPAAMYREFGFGLNRVALGDSDRAIGICGLLQRATLAAPDLGFALLP
                     AWRGQGYALEAARAVLEHARETLALPRVLAIVDAQNTPSINLLSRLGFERSGEHSDET
                     GSRALDLYALDLHAAGDPGDERVANRTRRPARAAGI"
     gene            3468..3929
                     /locus_tag="VIW27_04545"
     CDS             3468..3929
                     /locus_tag="VIW27_04545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5738968.1"
                     /translation="MSGSRIALAALLALLVFSGPAPAADDYALRDLDGKLHRASEHRG
                     KWLVINFWATWCLPCLRAMPELEDYYRANRTRVEVWGVTFEDSELAKILEFVERVGVS
                     YPILGYGQSPQTGYGKVRVLPTTFVVDPEGELHHRFEGPITAADLRRVIGD"
BASE COUNT          806 a         1355 c         1295 g          680 t
ORIGIN      
        1 atgatcgcgc gcctgatgcc gctgatggaa aaaggcgaag tcgctaatgc cagcaagctg
       61 gcgcgcgagt cgaatgccac ggtaagccga atcctgctgc agggcatgga gcactacaag
      121 tcggcgcggc ggcgtgacga cttcgaggcg gtgatggacg agagcgtcat ggaggcgatc
      181 ccgcggctcg agaaacgggt gcactacctg gcgatgttcg ccaacatggc gaccctgctc
      241 ggcctgctcg gcaccatcgt cggcctgatc aaggcgttta ccgcggtcgc ccaggtcgac
      301 ccgtcgatga aggcggaaat cctctccacc agtatctcgg tggcgatgaa taccaccgcc
      361 ttcggactga tcgtggcgat tccgctgctg ctgttctaca ccatgctgca aaccaaggcc
      421 accgagatcg tcgataacct cgaaactacc gtcgtcaagt tcgtcaacct gctgtggcgg
      481 gtcaagagcc gccagtaggc agaagcatgt cgaaacgaac tgccagaatg cggcatccgg
      541 ccgcggagct gaatatcacc gccttcatga acctgatggt ggtgctggtg ccgtttttgc
      601 tgatgacggc ggtcttcacc cagatcaccg tgctcgatct gaagctgccg ccgcccgacg
      661 ccctgaacaa gccccagccc gagcaaaagc cctacgagct gcggctgacg attcgcgccg
      721 acagcctggt gttgtccgat aaccgcgtcg gcctgatcaa gcagtttgcg cgccgcgacg
      781 gcaagcacga cttcgcgcag ttgaaatcgg cgctcaagca ggtcaaggcg cggatccccg
      841 cgcataccgg gatcaccatc ctgtccgagc agcagactcg ctacaaggac ctgatcgagg
      901 cgatggacag ctcgcgttcc tatcgcaccc aggtcgaggg cgaatgggta ttcgccgagc
      961 tgttcccgaa tatttccatc ggcgacgcgg cccggcaggt agcgaaatga gtacccggcg
     1021 cgacaaacgc aaacagaaat tcaaggcccg tcggcggccg cgcgcgaccc tgctcaacct
     1081 ggtatcgctg atggatatct tcaccatcct ggtgttcttc ctgctggtca acgcctccac
     1141 cgccgaggtc ctgccgtcgc caaagaatat cctcctgccc gaggccgggg ccgacaagct
     1201 gccggctctc aacctggtga tcgcggtcga cgaccgcgtg attcggctgc agggcaagca
     1261 ggtgatcgcg gtcagcgacg tactcgagga cggcaagtcg tccatcaggc cgctctacga
     1321 tgcgctgctc gaagcgcgca aaaccgtcaa gaacctgacc gacaagcaag gcgtgaccat
     1381 catgggcgac caggaaatac cctatgcgct gctgaaaaag atcatgctga cctgcaccgg
     1441 ggccgagtac accaatatct cgttcgcggt cattcagaag gtggcggaat cctgatgtcg
     1501 gcgaccgcga ctgccagggc cggcgatatg ctgtggatgg atcaccagga agaccgcggc
     1561 ttccgctggc tgtcgatctt cctgctggtg ctgttcctga ccggcggtgt agtgctgaat
     1621 tcgatcgaat tgccggaggt ggaacagaag cgactggtgg acgtctcgcc gcggctggcc
     1681 aagctgattc tcgaaaagca aaaggaacca ccgccgccac cgccggcccc gaagccgaaa
     1741 aaagaacaag ccaaaaaaga aaaaccggcg gagaagaaaa agccaccgcc gaaaaaggcc
     1801 gaaaaggcgc cgccgaagaa aagcgcccgc gaggtcgcgc agcaaagcgg cctgatcgcg
     1861 atggccgacg aactcgccga cctgcgcgac agcttcgcgc tcgacgatct cgccgcgctg
     1921 ccgcaaacca gtgccggcaa gcaggcgatc gaggtcgcgt cgaccgacca gctgctgact
     1981 gccggtgccg gccgctccag cggcggcatt cagaccgaca ccctgaaccg caagatcacc
     2041 accagcgaac tggcccagcg caggaccacc agcgtcgaga gcgctatcga agccgccgcc
     2101 ggcaaaagcg tcgcttcgag ctcctccgcg acgaggcaaa aagacctgtc gacggtgcgc
     2161 agcagggacg agatcgagcg cgtctttcaa cagtacaagg gggggatctt caatatctac
     2221 aaccgcgcgc tgcgcaagga cccgacactg gccggcaagg tagtcatcga gctgaccatc
     2281 gcgcccgacg gcagcgtcaa ggcggccagg atcctgtcga gcgaactcgg cgacgaacag
     2341 ctcgaacgca agctgatcct gaagatcaag cgcttcaagt tctcgaagtc gaacgtcgcc
     2401 gaaatcaccg tcacctaccc gatcgaattc ctgccctcct gacgcggcga cgccaatcca
     2461 gtcgccccgc gcccgccgta ataactgatg cggctcggcg gttctcgcgc agcgctgtcg
     2521 gatgtccgca accagcgttc gggccagccg ttcggcagat gccaccaggt cggcgaaaac
     2581 ctcaagctcg gtgtcggcca cgacttcacc gactttgccg acggctcgcc cgatctcgat
     2641 ttcgacaacc aggatttctt cggcaacgtg accagcaagc tctagcttgc gccgtggcgc
     2701 cgcgcccgcg atcctggtcc ctgcggtgca ggctgcaatc cactgcggcg actcgctttg
     2761 ccagcccgtc gacagcgtgc gacgagcccc ggccccggcc ccggccccgg ccccggccct
     2821 cggcgaggcg aggcgaggcg aggcaatcgg tttacggggg acggggccgt ccgcaaactg
     2881 gcagcggctt gctgccgggc ttatcattga tcttttgctg ttagcaatca tgagcgcacc
     2941 gttcgccatc gccacttcga gactgcgact acgctggctg accgcgagtg atgcggattt
     3001 cgtgctgcgc ctggtcaacg acgccgagtg gctgcgcttc atcggcgaca agggcgcgcc
     3061 ggacctcgat ggtgcgcgtc gctatatcga ggccggcccg gcggccatgt accgcgagtt
     3121 cggcttcggt ctcaaccgtg tggcgctcgg cgacagcgac cgcgcgatcg gtatctgcgg
     3181 cctgctgcaa cgcgccacgc tggccgctcc cgatctcggt ttcgcgctgc tgccggcctg
     3241 gcgcgggcag ggttacgcgc tggaggcggc gcgcgcggta ctcgaacacg cacgcgagac
     3301 gctcgcgctg ccgcgcgtgc tggcgatcgt cgatgcgcaa aacactccat cgatcaactt
     3361 gctgtcgcgc ctcggcttcg agcgttcagg cgagcacagc gacgagaccg ggtcgcgggc
     3421 actggatctg tatgcgctcg acctgcacgc ggcgggggac ccgggcgatg agcgggtcgc
     3481 gaatcgcact cgccgccctg ctcgcgctgc tggtatttag cgggccggcg ccggcggccg
     3541 acgattacgc gctgcgcgat ctcgacggca agctgcaccg ggcgtcggag catcgcggta
     3601 agtggctggt gatcaacttc tgggcgacct ggtgcctgcc atgcctgagg gcaatgcccg
     3661 agctagaaga ctactaccgc gccaaccgca cgcgggtcga ggtctggggc gtgactttcg
     3721 aagatagcga gctggcaaag atcctcgagt tcgtcgagcg ggtcggcgtc agctacccga
     3781 tactcggtta cggccagtcg ccgcagactg gctatggcaa ggtgcgcgtg ctgccgacga
     3841 ccttcgtggt cgaccccgag ggcgagctgc accaccgctt cgagggaccg attaccgccg
     3901 ccgacctgcg gcgcgtcatc ggcgattgac tcgacgccgc gcgcgattgc ctggatcctg
     3961 cgctatcctg tatatatacg tctgacgaac aatcccggag ccccgcatgt ccaagctggt
     4021 cagtgccgaa caagccgtcg ccagaattcc cgacgacgcc acgctggcga ccgccggctt
     4081 cgtcggcatc ggttttcccg aggcgctggc gatcgcgctc gaaaagcgtt ttctgg
//
LOCUS       DATCZV010000202         6832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_597613, whole genome shotgun sequence.
ACCESSION   DATCZV010000202 DATCZV010000000
VERSION     DATCZV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597613"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1279
                     /locus_tag="VIW27_04550"
     CDS             44..1279
                     /locus_tag="VIW27_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY5738969.1"
                     /translation="MQTKSLLGLIVVIIALVGGAFWLGINQGIDLSDTELRPQPPAPT
                     SVADAAPTPPAAAATDQTAESAAAAAAPKPGFTHFRVGNRNVKSIFAEDDKIWVGTSG
                     GVIRYLPATDEYRLFDVRNGLLANGVFHVSRWTAAKMLVGTYGGGLAVYDEIEDKFDI
                     FNVPEGLADAFVYDVLVMPNGDLWIATWSGANLVRGGRLEDPEAWETYTVENTGNGLP
                     NDWVYGLELGKDGEVWFATEGGLARLQDGKWANWNHQDGQGAPYEMVKNDNTFNRDPA
                     AMSNHHAKQKIEMGLEDVDTAYNPNYIVAIAVDRDGIVWVGTWGGGLAKFDGDQFTNY
                     TVADGLPSNHVFMLHEDSDGTLWVGTSHGLTTFMDGKFGHRLTTHEGLFSNTIFSMDT
                     QQDGLLWVGSFGGVTRLKM"
     gene            complement(1304..1900)
                     /locus_tag="VIW27_04555"
     CDS             complement(1304..1900)
                     /locus_tag="VIW27_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5738970.1"
                     /translation="MSRGRFPRFQQARRRYLGAGLLLLGLVVLGGWLLSDWITQPDYL
                     PLARESSLPPAATGVGELFELAVERMQQRDYPAALQLWHRVLLQSPENPQVKVNMGFT
                     LIELGNYAAAGDFFSSAVEQNPYQANAYYGLALVSEQAGDLEMALGAMRSYIHLAGAD
                     QDERFLRRARAALWEWEERLTAAREAEADSGAAADAAQ"
     gene            complement(1900..2694)
                     /gene="ccsA"
                     /locus_tag="VIW27_04560"
     CDS             complement(1900..2694)
                     /gene="ccsA"
                     /locus_tag="VIW27_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5738971.1"
                     /translation="MPKVDEFLLLNLACGFYLAAMIPAYLDRRRFPYALLIAACGLGL
                     ASHSLSLALRWVRVGHGPFINLYEILSSNLWSLMLGLTVFLLLARRHREAMGYVIPLP
                     AMLMAWLLLTPSKDTYLPATYDTVWLYFHVLTGKFFLALLMLATGLAVYEYRHRAGAL
                     ESAATAAFESVSYRLLAFAFCFQSLMLLFGAVWAQDAWGRYWAWDPLETWAFVTWVSV
                     IFVLHWRLNHAGQGIYALLVGTSLALAILTFFGVPFISTAPHQGMI"
     gene            complement(2758..4626)
                     /locus_tag="VIW27_04565"
     CDS             complement(2758..4626)
                     /locus_tag="VIW27_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5738972.1"
                     /translation="MASPLKQFLAVSIIGVTLTVAALLYLHVQLTEGYIHTHLDSHNE
                     NLAIVLRNSALQTGLEQELAADRDTLSAPMLAILGERLDRELQRIPVLKVKIYNRNGI
                     VVYSTKADEIGEDATHNPGFLSALGGVPASSKVDPDHVNEFDRVVEIDNLHQQYIPMT
                     MLDSEQPIGVFETYLDINDIVASVESMQRTMFWLMGGLLAAIYLALAATFLRTHRLLQ
                     EERYQHEAHLRALMEMRETLENRVAERTAELDHARHFLQATMDNVPDAILTCDAEFVI
                     ESANLSALELFNLEESQLAGRKLSEFFPDDGDGELLQLAATEHQQTVLKPEGGEVVPV
                     DLWQGPLANGNGLNRHIVLIRDITGEVRAQRELEKTRKQYFHQEKMAAIGQLAAGILH
                     EVGNPIAAIAGAAGEVQASLGRDDSDVDVAQNIELINRQILRLGKITSDIAEFASPKP
                     RERELLDLNGLLRSTANLLTYDRRFDKMGVRLELDKNLPAIVGVADQLTQVFMNLLIN
                     SMDACVSKKSLEDCIVVSSAMDGDRVHVAVEDSGSGMSAETLEHVMETFFTTKPVGEG
                     TGLGLSLCDTIVSAHGGELKIRSEQGKGTLVDVFLPVELPDDAAPEELAPERRDHQ"
     gene            5005..6156
                     /locus_tag="VIW27_04570"
     CDS             5005..6156
                     /locus_tag="VIW27_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5738973.1"
                     /translation="MALIRRARRFGLLPVAACLLALLWLTLPALDARAQYAGPGVIAD
                     RPATTYERLLPLAEEGDPQARHLLGYMYFYGEGVELDYDQAHQWFHLAAEEGEPKSMR
                     NLGLYHAHAISRIPARFYDPREANLWFSLAATAAANPVVASAADASYDRFLASDKVSL
                     LAAAAPGELGETVYTAHCAGCHGFDGHSSYIHAPSFALGESLHKSDTQLLISIGRLLD
                     AWPHQLEGIPRQAQQAALTYIREQLGGKPTRVGLSSNLLPAVSSSPTRARAQHGPGAE
                     IYPRFCGGCHGFNGIATYVNSPSFALGERLHKSDDELAATIRNGRGEMPSWEYMLQPA
                     EIKSLVSYLRSFEPALQLGIDGGLHIDPERFFQFKPRSLGGPGNLRGTQ"
     gene            6325..>6832
                     /locus_tag="VIW27_04575"
     CDS             6325..>6832
                     /locus_tag="VIW27_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY5738974.1"
                     /translation="MPVNNKRISLPRQLVKRDGRQVSFEPLRIAVAIEKAGQATGEFE
                     PPEARRIARQVLRVIAHRDDDGSLPGVEAVQDICEQALIAANHFKTARAYIVYREQHR
                     KLRADRRTLLDVASSINEYLDRSDWRVKANANQGYSLGGLILNTSGKMIANYWLNHVY
                     PPEIGEAHR"
BASE COUNT         1309 a         2260 c         2059 g         1204 t
ORIGIN      
        1 cggctggcca tgcaaccctc gcaatcactc tcgagctccg ataatgcaaa ctaaatcact
       61 gctaggccta atcgtcgtca tcatcgccct ggtcggcggc gccttctggc tgggtatcaa
      121 ccagggcatc gatctcagcg acacggagct tcggccacag ccccccgcac ccacctcggt
      181 agctgacgcg gcgccgacac cgccggccgc cgcggcaacc gaccagacag cggaatcggc
      241 ggctgccgct gcggcaccga aacccggctt tacccacttt cgagtcggca accgcaacgt
      301 caagtcgatc ttcgccgagg acgacaagat ctgggtcgga acctccggcg gcgtgatccg
      361 ctacctgccc gcgaccgacg aataccggct gttcgacgtg cgcaacggcc tgctagccaa
      421 cggcgtgttt cacgtcagcc gctggaccgc cgccaagatg ctggtcggaa cctacggcgg
      481 cggcctcgcc gtttacgacg agattgagga caagttcgac atcttcaatg tgccggaagg
      541 actggccgat gccttcgttt acgacgtgct ggtgatgccc aacggcgacc tgtggatcgc
      601 gacctggtcg ggtgccaacc tggtcagggg tggccgcctg gaggatcccg aagcctggga
      661 aacctatacc gtcgaaaata ccggcaacgg cctgcccaac gactgggtat acggcctgga
      721 actgggcaag gacggcgaag tctggtttgc gaccgagggc ggcctggcgc gacttcagga
      781 cggcaaatgg gcgaactgga accaccagga tggtcagggc gcgccctacg aaatggtcaa
      841 aaacgacaat actttcaacc gtgatccggc ggcgatgtcg aaccaccacg ccaagcaaaa
      901 gatcgaaatg gggctcgagg acgtcgacac cgcctacaac ccgaattaca tcgtcgccat
      961 cgccgtcgac cgcgacggca tcgtctgggt cggcacctgg ggcggcgggt tggcgaagtt
     1021 cgacggcgac cagtttacca attacacggt cgccgacggg ctgccttcga accacgtctt
     1081 catgctgcac gaggacagcg acggcaccct gtgggtcggc accagccacg gcctgacgac
     1141 cttcatggac ggcaagttcg gccaccgcct gacgacccac gagggcctgt tttcgaatac
     1201 cattttctcg atggataccc agcaggacgg actcctctgg gtcggcagct tcggcggcgt
     1261 cacccgcctg aaaatgtaac cggccgtcgc ctgccggccg cgctcattgc gcggcgtcgg
     1321 ccgctgcgcc gctgtcggcc tcggcttcgc gcgccgcggt cagccgctct tcccactccc
     1381 acaacgccgc gcgcgcgcgg cgcaggaaac gctcgtcctg gtcggcgccg gccaggtgga
     1441 tgtagctgcg catcgcgccc agtgccatct ccaggtcgcc ggcctgctcg ctgaccagcg
     1501 ccagcccgta ataggcattg gcctgatacg gattctgctc caccgcgctg ctgaaaaaat
     1561 ccccggcggc ggcgtaattg ccgagctcga tcagcgtgaa ccccatgttg accttgacct
     1621 gcggattttc cgggctctgc agcaacacgc ggtgccagag ttgcagcgcg gccgggtagt
     1681 cgcgttgctg catgcgctcg acggccagct cgaacaattc gccgaccccg gttgccgccg
     1741 gcggcagcga cgactcgcgc gccagcggca ggtagtcggg ctgggtgatc cagtcgctga
     1801 gcagccaacc ccccaacacc accaggccca gcagcaacag gccggcgccg agataacgcc
     1861 ggcgcgcctg ctgaaagcgc ggaaagcgcc cccgggacat caaatcatgc cctggtgcgg
     1921 cgcggtagag atgaagggca cgccgaagaa ggtcaggatg gcgagcgcca ggctggtgcc
     1981 caccagcagc gcgtaaatgc cctggccggc atgattcagg cgccagtgga gcacgaaaat
     2041 gacgctgacc caggtcacga aggcccaggt ttcgagcgga tcccaggccc agtacctgcc
     2101 ccaggcgtcc tgtgcccaga ccgcgccgaa cagcaacatc agcgactgga aacagaacgc
     2161 gaacgccagc aagcggtaac tgaccgactc gaacgcggcg gtggccgctg actccaacgc
     2221 gccggcgcgg tgacggtact cgtagaccgc gagtccggtc gccagcatca acagggcgag
     2281 aaaaaacttg cccgttagca catgaaaata cagccacacg gtatcgtagg tggcgggcag
     2341 gtaggtgtcc ttcgacggcg tcagcaacag ccaggccatc agcatcgcgg gcagcgggat
     2401 cacgtaaccc atcgcctcgc gatgccgtcg cgccagcagc aggaacacgg tcaggccgag
     2461 catcaggctc cacaggttgc tgctgaggat ttcgtataga ttgatgaacg ggccatgacc
     2521 gacgcgcacc cagcgcagcg cgaggctgag actgtggctc gccagtccca gtccacaggc
     2581 ggcgatcagc agcgcgtacg gaaaccgccg ccggtccagg taagcgggaa tcatcgcggc
     2641 caggtaaaaa ccgcaggcca ggttcaacag caggaattcg tcgactttcg gcattgggtt
     2701 tatctcgatt tcgggcttgc gcgcgacgaa aaaccgggca ggtccgccgt gcaacggtta
     2761 ttgatgatcc cggcgctccg gcgccagctc ctcgggcgcc gcatcgtccg gcagttcgac
     2821 cggcaggaac acgtcgacca gagtcccctt gccttgctcg ctgcgaatct tcagctcgcc
     2881 accgtgagcg ctgacgatgg tatcgcacaa tgacaggccg aggccggtgc cctcgccgac
     2941 cggcttggtg gtgaaaaacg tttccatgac atgttcgaga gtctccgccg acattcccga
     3001 accgctatcc tcgacggcga catgcaccct gtcgccgtcc atcgcgctgc tgacgacgat
     3061 gcaatcctcg agagattttt tcgacacgca ggcgtccatc gagttgatca gcaggttcat
     3121 gaacacctgg gtcagttgat cggcgacgcc gacgatggcc ggcaggttct tgtccagctc
     3181 gaggcgcacg cccatcttgt cgaagcggcg gtcataggtc agcaggttgg ccgtacttct
     3241 gagcaaaccg ttgaggtcca gcaattcccg ttcgcgcggt ttcggcgacg cgaactcggc
     3301 gatatcgctg gtaatcttgc cgagcctcaa gatctggcga ttgatcagtt cgatattttg
     3361 cgccacgtcg acgtcgctat cgtctctgcc gagcgaggcc tgcacttcac cggcggcgcc
     3421 ggcgatcgcc gcgatcgggt tgccgacctc gtgcagtatg ccggccgcaa gctgaccgat
     3481 ggccgccatc ttttcctggt gaaagtattg cttgcgggtt ttttccagct cccgctgggc
     3541 tcttacctcg ccggtaatat cgcgtatcag gacaatatgc cggttaagcc cgttgccgtt
     3601 ggcgagcggt ccctgccaca ggtctaccgg gacgacttcc ccgccctcgg gtttcaacac
     3661 cgtctgctga tgctccgtgg cagccagttg cagcaattcg ccatccccgt catcgggaaa
     3721 aaactcgctc agtttcctgc ccgccagctg cgactcttcg aggttgaaca attccagcgc
     3781 ggaaaggttg gcgctttcga tgacgaactc tgcatcacag gtcagtatcg catcgggaac
     3841 attgtccatg gtcgcctgca gaaaatgcct ggcatgatcg agttccgcgg tgcgctccgc
     3901 cacgcggttc tcgagggttt cccgcatctc catgagtgcg cgcaggtgcg cctcgtgctg
     3961 ataccgctct tcctgcaaca aacggtgtgt gcgcagaaag gtggccgcca acgccaggta
     4021 gatcgccgcg agcaaaccgc ccatcagcca gaacatggtt ctctgcatcg attccacgct
     4081 ggcgactatg tcgttgatgt cgagataggt ctcgaatacc ccgatcggct gctcggagtc
     4141 gagcatggtc atgggtatgt attgctggtg aagattgtcg atctcgacga cgcgatcgaa
     4201 ctcgttcaca tggtccgggt cgaccttgct ggatgccggc accccaccga gcgcgctcag
     4261 gaagccggga ttatgggtgg catcttcacc gatttcatcc gccttcgtcg agtagacgac
     4321 gatgccgttg cgattgtaga tcttgacctt gagcaccgga atccgctgca gctcgcgatc
     4381 gagccgttcg ccgaggatgg caagcatcgg tgccgacagc gtatcccgat ccgcggccag
     4441 ctcctgttcg aggccggttt gcagggccga attgcgcagt acgatcgcga ggttctcgtt
     4501 gtgcgaatcg agatgcgtat gaatgtagcc ctcggtcagc tgcacgtgca ggtacagcag
     4561 cgcggctacc gtcagcgtga cgccgatgat actgacggcc aggaactgtt tcagcggcga
     4621 agccaacgca cataccctgc aggtgaactg ccgctcactg aatttgcctc gaacatgggg
     4681 agcacttctc gaatcaatta ccgaattcta agatagatag ccgcagactg cattcaattg
     4741 acatgggaca cccccggttt aagtttgcga cccgcattct actaccgatt gcgcaactcg
     4801 ggtaacgccc gggcaaggcg cacaggcccg cccgcggcgg ccggacctcg aaccccgtcc
     4861 caggggagcg tagccgggaa actgcctcac cggctttcgg cctcgcttta ccgtgccgat
     4921 accactgctt ataattcggc tcatgtacct gcagtcgacg cgcgcccgag acctcccggc
     4981 caaccaacgg cgctggcggt tgccgtggcg ctgatccgtc gcgccaggcg cttcggcctg
     5041 ctgccggtgg ccgcctgcct gctggccctg ctgtggctga ctctccccgc tctcgatgcc
     5101 cgcgcccagt atgccggccc tggcgtgatc gccgaccgcc ccgccaccac ctacgaacgc
     5161 ctgctgccgc tggccgagga aggcgatccg caagcccggc atttgctcgg ctacatgtat
     5221 ttctacggcg aaggcgtcga actggattac gaccaggcgc accagtggtt tcatctggcc
     5281 gccgaggaag gcgagccgaa gtcgatgcgc aacctcggcc tttaccacgc ccacgccata
     5341 tcgcgcattc cggcccgctt ctacgatccc cgcgaggcca acctgtggtt cagcctggcg
     5401 gcgaccgctg ccgccaatcc cgtggtcgca tccgccgccg atgccagcta cgaccgcttc
     5461 ctcgccagcg acaaggtatc gctgctcgcc gccgccgcgc cgggcgagct cggcgaaacg
     5521 gtttataccg cgcattgcgc cggttgccat ggcttcgacg gccattcgag ttacatccat
     5581 gcgccttcgt tcgcgctcgg cgaaagcctg cacaagagcg acacccagct gctgatcagc
     5641 atcgggcgcc tgctcgatgc ctggccgcat cagctcgaag gtattccacg gcaggcgcag
     5701 caggccgcgc tcacctatat tcgcgagcaa ctgggcggca agccgacccg ggtcgggctg
     5761 tcgtcgaacc tgctgcccgc ggtctcgtcg tcgccgacgc gggcgcgcgc ccagcatggt
     5821 cccggagcgg agatctatcc ccgcttctgc ggcggctgcc acggcttcaa cggtatcgcc
     5881 acctacgtca attcaccttc attcgcgctc ggcgagcgcc tgcacaagag cgacgacgag
     5941 ctggcggcga cgatccgtaa tggccgcggc gaaatgccgt cgtgggaata catgctgcag
     6001 ccggcggaga taaagtcgct ggtaagctac ctgcgcagct tcgaacccgc cctgcagctg
     6061 ggtatcgacg gcggcctgca catcgatccc gagcgctttt ttcagttcaa accgaggagc
     6121 ctgggcgggc ccggaaacct gcgcggcacg cagtagcaga actgagccgc gacgcccgta
     6181 ccgccgccgg cgcaggccgc gccgcgaccg ttccgaatcc gccgcgaggg gtaaaatgcg
     6241 gttcaattga cataagtcaa ttaattcgtc gaaactacta tataaagtgt tcggtctgat
     6301 aataaaacac aatatatagt agccatgcca gtcaacaaca aacgaatttc cttaccccgg
     6361 cagctcgtca agcgcgatgg ccgacaggtc tcgttcgagc cgctgcggat cgccgtcgcg
     6421 atcgaaaaag ccggccaggc caccggcgaa ttcgagccgc ccgaggcgcg ccggatcgcg
     6481 cgccaggtac tgcgggtcat cgctcaccgc gacgacgatg ggagcctgcc cggggtcgag
     6541 gcggtgcagg acatctgcga gcaggcgctg atcgccgcca accatttcaa aaccgcgcgc
     6601 gcctacatcg tctaccgcga gcagcatcgc aagctgcgcg ccgaccggcg cacgctgctc
     6661 gacgttgcca gttcgatcaa cgagtatctc gatcgttccg actggcgggt caaggccaac
     6721 gccaaccagg gttactcgct gggcggcctg atcctcaaca cttcgggcaa gatgatcgcc
     6781 aactactggc tgaaccacgt gtatccgccg gagatcggcg aagcccatcg cc
//
LOCUS       DATCZV010000203         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_545373, whole genome shotgun sequence.
ACCESSION   DATCZV010000203 DATCZV010000000
VERSION     DATCZV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545373"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VIW27_04580"
     CDS             <1..299
                     /locus_tag="VIW27_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738975.1"
                     /translation="PADSGSGWRHPGGADGIVLDARGNATGSGAKSLGVELANSRAFA
                     QCQVDKAFEAVCLRDPNQLAADRAARDSFVGNFSGGGFNMREVFTDVAAYCKGN"
     gene            302..>2418
                     /locus_tag="VIW27_04585"
     CDS             302..>2418
                     /locus_tag="VIW27_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY5738976.1"
                     /translation="MPGPYRASRPIAALVIAAALAACDTGGVGTSANPDLGTGNSGFV
                     YSGPAARDVDVSNFQFYLYNNLNPDNRCGGCHNSQASTPVSPLFFDTTDVNAAYNASN
                     AYVDLDNPPASGFVQQMNGGHFCWEPVSSICGTLIAGWIEDWKNAANGGIASRQINLQ
                     PPENIRPPGQTRSFPAGPDDNDPNSFAQTVYPVLSGTLGSIVGDNCQGCHSETAPGLP
                     QAPFFASGDVAAAYAAARSKMNIDTPENSRLVVRVRDGHNCWSNSCDSDADLLEQAIR
                     DFALAIALEDIDLALVTSMALQLGDGIIASGGSRHETDQFAIWEFKTGSGLTAFDTSG
                     IDPSIHLSLIGSVDWVGGYGLEFSGGRAQADTISSDKLYTYIRTTGEYALEAWVIPSN
                     VSQENTNIVSYSGGSATRNFTLAQTLYDYEAYNRIDSVQPQAVNGVPFLTTGQNGLEI
                     AQASLQHVVVNYDPFVGRSIYVNGEPVPGLTDPAAAPTSIANAWDDGFAFVLGGEIGG
                     SRAWSGQIKLVAMHNRKLTAEQIRQNFEVGVGQKFFLLFWVGQHLGEDPADPQSFILF
                     EVAQFDSFGYLFNRPTFINLDPTWVPSGSIAIRGLRIGINSSEAIAGQAYANLDTSVD
                     AGNYDPGQGRLLSPLGTVIALDKGPQSDEFFLTFETFAGRSNAFTDIQPTLPADPADP
                     PTPPAADIGLRTFEEINSAIAEIT"
BASE COUNT          452 a          845 c          726 g          395 t
ORIGIN      
        1 gcccggccga cagcggtagc ggctggcggc acccgggcgg cgccgacggc atcgtcctcg
       61 acgcacgcgg caacgccacc gggtcgggcg ccaagtcgct gggcgtcgag ctcgccaaca
      121 gccgcgcctt cgcgcaatgc caggtcgaca aggccttcga agcggtgtgc ctgcgcgatc
      181 ccaaccagct cgcggcagac cgcgccgcgc gcgactcctt cgtcggcaat ttcagcggcg
      241 gtggcttcaa catgcgcgag gtatttaccg acgtggcggc ttactgcaaa gggaattgac
      301 gatgccaggc ccataccgtg caagcagacc gatcgccgcc ctggtgattg ccgccgcgct
      361 tgccgcctgc gacaccggcg gcgtcggcac cagtgccaac cccgacctcg gcaccggcaa
      421 tagcggtttc gtctacagcg ggccggccgc ccgtgacgtc gacgtcagca atttccagtt
      481 ttacctgtac aacaatctca acccggacaa tcgctgcggc ggctgccata actcgcaggc
      541 ttcgacccct gtcagcccgt tgtttttcga caccaccgac gtcaacgcgg cctacaacgc
      601 atcgaacgcc tacgtcgatc tcgacaatcc gccggcctcg ggtttcgtgc aacagatgaa
      661 cggcggccac ttctgctggg agccggtgtc gtcgatctgc ggcacgctga tcgccggctg
      721 gatcgaggac tggaaaaacg ccgccaacgg cggtatcgca tcgcggcaga tcaacctgca
      781 gccgccggag aatattcgcc cgcccggcca gaccagatcg tttccggccg gccccgacga
      841 caatgacccg aacagtttcg cgcaaaccgt atacccggtg ctgagcggta cgctcggctc
      901 catcgtcggc gacaactgcc agggctgcca tagcgagacc gcaccgggcc tgccgcaggc
      961 gccgtttttc gccagcggcg acgtcgccgc ggcctacgcg gccgcgcgct cgaaaatgaa
     1021 tatcgacacc ccggagaatt cacgtctggt ggtgcgcgtg cgcgacggcc ataactgctg
     1081 gtcgaacagt tgcgacagcg acgccgattt gctcgagcag gcgatccgcg attttgcgct
     1141 cgccatcgcg ctggaggata tcgatttggc gctggtcacc agcatggctc tgcagctcgg
     1201 cgacggcatt attgcctccg gcggcagccg tcacgaaacc gaccagttcg cgatctggga
     1261 attcaagacc ggcagcgggt tgaccgcgtt cgataccagc ggcatcgacc cgtcgatcca
     1321 cctgtcgctg atcggcagcg tcgactgggt cggcggttac gggctggaat tcagcggcgg
     1381 gcgcgcgcag gcggacacca tctccagcga caagctgtat acctacattc gaaccaccgg
     1441 cgaatatgcc ctcgaagcct gggtcatccc gtccaatgtc agccaggaaa ataccaacat
     1501 cgtcagctat tcgggcggca gcgcgacgcg caacttcacg ctcgcgcaga cgctctacga
     1561 ttacgaggcc tacaaccgca tcgactcggt gcagccgcag gcggtcaatg gcgtaccctt
     1621 tctgaccacc gggcagaacg gcctcgagat cgcacaggcc agcctgcagc atgtggtcgt
     1681 caattacgac ccgttcgtcg gccgcagcat ctacgtcaac ggcgagccgg tgcccgggct
     1741 gaccgatccg gccgcggcgc cgacctcgat cgccaacgcc tgggacgacg gcttcgcctt
     1801 cgtgctcggc ggcgagatcg gcggcagccg cgcctggagc ggccagatca agctggtcgc
     1861 gatgcacaac cgcaagctga cggccgaaca aatccggcag aatttcgagg tcggcgtcgg
     1921 gcagaaattt ttcctgctgt tctgggtcgg ccagcacctc ggcgaagacc cggcggaccc
     1981 gcagagcttc attctcttcg aggtcgcgca gttcgacagc ttcggctacc tgttcaaccg
     2041 gcccaccttc atcaatctcg acccgacctg ggtgccgtcg ggctcgatcg cgattcgcgg
     2101 cctgcgcatc ggcatcaaca gcagcgaagc catcgccggc caggcctatg caaatctcga
     2161 caccagcgtc gacgccggca actatgatcc cggccagggc cggctactgt ccccgctcgg
     2221 caccgtgatc gcgctcgaca agggcccgca gagcgacgag tttttcctga ccttcgaaac
     2281 cttcgctggc agaagcaacg catttaccga tatccagccg acgctaccgg ccgatcccgc
     2341 cgatccacca accccgccgg cggccgatat cgggctgcgc accttcgagg aaatcaacag
     2401 cgcgatcgcc gagatcac
//
LOCUS       DATCZV010000204         5805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_174517, whole genome shotgun sequence.
ACCESSION   DATCZV010000204 DATCZV010000000
VERSION     DATCZV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174517"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VIW27_04590"
     CDS             <1..222
                     /locus_tag="VIW27_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5738977.1"
                     /translation="LKALPVLILGGFTSVPGAIIGGIIIGVGEKVAEVYAGPFVGGAI
                     ENWFAYVLALAFLLVRPQGLFGEKIIERV"
     gene            389..1465
                     /locus_tag="VIW27_04595"
     CDS             389..1465
                     /locus_tag="VIW27_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5738978.1"
                     /translation="MIYRETGQFKASYQADQALFPIAQDRFVVAGILLVVFALVPYLL
                     NEYWLNAILLPFLIYALAALGLNILVGYCGQLSLGTGAFMAVGAFSIYKLMVAMPELG
                     FIPLVILSGLITAAVGTLFGLPSLRIKGFYLAVATLAAQFFLIWMFNKVGWFFNYSAS
                     GMINAPPMTVLGFEITGPTANPTAKYYLVAAIVTVFALAAKNIARGRIGRNWMAIRDM
                     DIAAELIGVRPLVAKLSAFAVSSFYIGMAGALYFAVWLGSAEATEAFDINQSFLVLFM
                     IIIGGLGSVLGSFLGAAFLVIMPILLKNIMVDYLGTDSALATHLEFIIVGVLIIAFLI
                     VEPHGLARLWRIAKEKLRLWPFPY"
     gene            1535..2848
                     /locus_tag="VIW27_04600"
     CDS             1535..2848
                     /locus_tag="VIW27_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5738979.1"
                     /translation="MKKLTTTIVAALLSLPLLSTNAAAYELVMPTLDYRTGPYAPNGI
                     PFANGYGDYLNMLNERDGGINGVRISLVPCETGYNTKRGVECYENTKGEGMSGALIYQ
                     PLSTGITYQLIPKASADEIPVFSMGYGRTSAANGKVFPWIFNFPATYWSQATVFVKYI
                     GEQEGGLANLKGKKIALVYHNSAYGKEPIRTLESAAAKYGFEFMQLPVDHPGQEQKAT
                     WLQIRRERPDWVLMWGWGVMNQVAIKEAASIRYPMDRFIGVWWSGSENDVLPAGDGAH
                     GYLSGAFHQPGGNFPVHDDIRKHVYDKGMGLGERERIGEVLYNRGMVAAMWSSEAIRK
                     AMDIHGTTEIRGTHVRDGLEALDVDEARLKELGMANFTIPVKITCENHEGPGKVALQQ
                     WDAKAKKWSLVTDFYEPMRDITGPLIAEDSAQFAAENNVEERSCN"
     gene            2876..3706
                     /locus_tag="VIW27_04605"
     CDS             2876..3706
                     /locus_tag="VIW27_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5738980.1"
                     /translation="MNQAANDIAASPLLAVNNIEVIYDHVILVLKGVSLEVPEQGIVA
                     LLGANGAGKTTTLKAISNLLRAERGDITKGSIEFGGQRIDRLSSAELVKRGVIQVMEG
                     RHCFEHLTVEENLLTGAYTRRDGHTAIQRDLELVYSYFPRLKRRCKAQAGYTSGGEQQ
                     MVAIGRALMSKPKIMLLDEPSMGLAPQLVEEIYEIVERLNREEGVTFLLAEQNTNIAL
                     RHADHGYILENGRVVLEGSAVSLRENNDVKEFYLGISGSERVNFREVKHYRRRKRWLT
                     "
     gene            3885..5120
                     /locus_tag="VIW27_04610"
     CDS             3885..5120
                     /locus_tag="VIW27_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5738981.1"
                     /translation="MSDFYDALETRSTEEREQAQLAALRAQIDHARQNTQAYAASLAG
                     VDAAGITDAAAFSALPLTRKSALIELQKAQRPFGGYAPTGGAEYSHVFASPGPIYEPG
                     YAVADFWRFARSLYAAGFRRGDLVHNTFSYHFTPAGQMLDSGARALGCPVIPAGTGQT
                     ELQVQTIADLKPAGYVGTPSFLKIILDKADELGSDVASIDKALVTGEALPPPLRESFA
                     ARGINVLQCYATADLGLIAYESDAREGLIVDEGVYLEIVRPGSGDPVADGEVGEVVVT
                     SLGREYPLIRFATGDLSAFMPGASPCGRTNRRITGWMGRADQTAKVRGMFIHPQQVDA
                     VVKRHPEIARARLVVDWVNQADEITLQCEVDDYNESLGAAIADSIRAICKLRGEVDLQ
                     APGSLPNDGKVIDDVRKYD"
     gene            5128..>5805
                     /locus_tag="VIW27_04615"
     CDS             5128..>5805
                     /locus_tag="VIW27_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5738982.1"
                     /translation="MTSRPLLAGVRVIDLSQYIPGPFATRQLADLGADVIKIEPPGGD
                     PMQHFMATENDEPSPVYRHINRGKRICRVDLKQDDGQRILAGLLENADILLESFRPGV
                     LARLGFDRDRLDAINPRLIHCALSGFGHTGPYAQRAGHDINYCALAGQSLVSGTAERP
                     VIGFPPIADHASAQQAAIAMLAALHGRENRDGGIFLDISISESILAWQYLPALGGASE
                     RAASILDG"
BASE COUNT         1112 a         1877 c         1792 g         1024 t
ORIGIN      
        1 ctgaaagcgc tgccggtact aatcctcggc ggcttcacct cggtgccggg tgcgatcatc
       61 ggcggcatca tcatcggtgt cggcgaaaag gtcgccgagg tttacgcagg ccccttcgtc
      121 ggcggcgcga tcgaaaactg gttcgcctac gtgttggcgc tggccttcct gctggtgcgc
      181 ccgcagggac tgttcggcga aaagattatc gagcgggtgt gatggcagtt ctgcaagaaa
      241 tgcttgtcgt ccgcgcgaac ctcgaactga tcgccgcgca agcggggatc cacagggccg
      301 cgacagcgat cgcgccggga tcgcggggcc tcgatacagc gagccgccgc tgtcgccgga
      361 tcgacttcag tagcctggaa accaaccaat gatctaccgc gagacaggcc agttcaaggc
      421 cagctaccag gccgaccagg cgcttttccc aatcgcccag gaccgcttcg tggtcgccgg
      481 catcctgctg gtggtgttcg cgctggtacc ctacctgctg aacgagtact ggctgaatgc
      541 gatcctgctg ccgtttttga tctacgcgct ggccgcgctg ggcctcaaca tcctggtcgg
      601 ctactgcggc cagttgtcgc tcggtaccgg cgccttcatg gcggtcgggg cgttctcgat
      661 ctacaagttg atggtggcca tgccggagct cggatttatt ccgctggtga tcctttccgg
      721 gctgatcacc gccgcggtcg gcaccctgtt cggactgccg tcgctgcgta tcaagggctt
      781 ttacctggcg gtggcgacgc tggccgcgca attctttttg atctggatgt tcaacaaggt
      841 cggctggttt ttcaattaca gcgcgtcggg gatgatcaat gcaccgccga tgaccgtgtt
      901 gggcttcgaa attaccggcc cgacggccaa tccgacagcc aagtactacc tggtcgccgc
      961 gatcgtcacg gtgttcgcgc tggcggcgaa gaacatcgcc cgcggccgca tcggccgtaa
     1021 ctggatggcg attcgcgaca tggacatcgc cgccgaattg atcggcgtgc gcccgctggt
     1081 ggccaagctg tcggcattcg cggtatcgtc attctacatc ggcatggccg gcgcactcta
     1141 cttcgcggtc tggctgggtt ccgcggaagc gaccgaggcc ttcgacatca accagtcctt
     1201 cctggtgctg ttcatgatca tcatcggcgg cctcggctcg gtgctcggct cgttcctcgg
     1261 cgccgccttc ctggtcatca tgccgatcct gttgaaaaac atcatggtcg actacctcgg
     1321 taccgacagc gcgctggcca cgcacctgga attcatcatc gtcggtgtgt tgatcatcgc
     1381 ctttctgatt gtcgaaccgc acgggctcgc gcgcctgtgg cgaatcgcca aggaaaagct
     1441 gcgcttgtgg ccgtttccct actaacgagt ggacgggtcc gcgcggcgta acatagtata
     1501 ttgcaacaac ataacaaccg gaggactgaa gataatgaaa aaactgacca caacgatcgt
     1561 ggcggcactg ctgtctctgc cgttgttgag caccaatgcg gccgcctacg aactggtgat
     1621 gccgacgctc gactatcgga ccggccccta tgcccccaac ggcataccgt ttgccaacgg
     1681 ctacggggat tacctcaaca tgctcaacga gcgcgacggc ggcatcaacg gcgtgcgcat
     1741 cagcctcgta ccctgcgaga ccggctataa caccaagcgc ggcgtggagt gctacgaaaa
     1801 taccaagggc gagggcatgt ccggcgcgct gatctaccaa ccgctgtcca ccggcatcac
     1861 ctaccagctg attccgaaag cctcggccga cgaaatcccg gtgttctcga tgggctatgg
     1921 ccgcacctcg gccgccaacg gcaaggtctt tccgtggatt ttcaacttcc cggcgaccta
     1981 ctggagccag gcgacggtat tcgtcaagta tatcggcgag caggaaggcg gactggccaa
     2041 tctcaagggc aagaagatcg ccctggtcta ccacaactcg gcctacggta aggaaccgat
     2101 ccgcacactc gaaagtgccg ccgcgaaata cggattcgaa ttcatgcaac tgccggtcga
     2161 tcatcccgga caggagcaaa aggcgacctg gctgcaaatt cgccgcgagc gtcccgactg
     2221 ggtgctgatg tggggctggg gcgtcatgaa ccaggtggcg atcaaggaag ccgcgtcgat
     2281 ccgttacccg atggatcgtt tcatcggcgt ctggtggtcg ggttccgaga acgacgtgct
     2341 gccggccggc gacggcgccc acggctacct gtcgggtgct ttccaccagc cgggcggtaa
     2401 tttcccggta cacgacgata tccgcaaaca cgtctacgac aagggcatgg gtctcggcga
     2461 gcgcgagcgc atcggcgaag tactctacaa tcgcggcatg gtcgcggcga tgtggagctc
     2521 cgaggcgatt cgcaaggcga tggatatcca cggcaccacc gagattcgcg gcacgcacgt
     2581 gcgcgacggc ctcgaggcgc tcgacgtcga cgaagcgcgc ttgaaggaac tcggaatggc
     2641 gaacttcacc ataccggtaa agatcacctg cgagaatcac gaggggccgg gcaaggtagc
     2701 cttgcagcaa tgggatgcca aggccaagaa gtggagcctg gtgaccgact tctacgagcc
     2761 gatgcgcgat atcaccggcc cgctgatcgc cgaggattcg gcccagttcg cggccgagaa
     2821 caacgtcgag gagcggagct gcaactagct ccggctcgcg ataccgggag tcaatgtgaa
     2881 ccaggccgcc aacgacatcg ccgccagccc gctgctggcg gtgaacaaca tcgaggtgat
     2941 ctacgatcac gtcatcctgg tattgaaagg cgtatcgctg gaagttcccg agcagggcat
     3001 cgtcgccctg ctcggggcca acggcgccgg caagacgacg accctgaagg cgatctccaa
     3061 cctgctgcgc gccgagcgcg gcgacatcac caagggcagc atcgagtttg gcggtcagcg
     3121 catcgaccgc ctgagttcgg ccgaactggt gaaacgcgga gtcatccagg tgatggaggg
     3181 ccgccattgc ttcgaacacc tgacggtcga ggaaaacctg ctcaccggtg cttacacacg
     3241 ccgcgacggc cataccgcca tccagcgaga cctggaactg gtctacagct acttcccgcg
     3301 gctcaagagg cgctgcaagg cgcaggcagg ctatacctcg ggcggcgagc aacaaatggt
     3361 cgccatcggc cgcgcgctga tgtcgaagcc gaagatcatg ctgctcgacg agccatcgat
     3421 gggcctcgca ccgcaactgg tcgaggaaat ctacgaaatc gtcgagcgcc tcaacaggga
     3481 agaaggcgtc accttcctgc tcgccgagca aaataccaac atcgcgctgc gtcacgccga
     3541 ccacggttac attctcgaaa acggccgcgt ggtactcgag ggcagcgccg tcagcctgcg
     3601 ggaaaacaac gacgtcaagg agttttacct tggcatcagc ggcagtgagc gggtcaattt
     3661 ccgcgaagtg aagcattacc gccgcagaaa aagatggctt acctagcgca ggggatccgt
     3721 caccccggcc cccgagccga aacgtcggac cgcggggtcc atcgtgatgc gcgacattcg
     3781 aaaaacacct tgaaaactca atgccgggat ttgagcgctg ctttagcgcc ttactgcagt
     3841 tcacatggat gccggctcgg cggccggcat gacggaggtt gaagatgtca gatttctacg
     3901 acgcgctgga aacacgcagc accgaggaac gagaacaggc gcaactggct gcgttgcgcg
     3961 cgcaaattga ccatgcccgg caaaacacgc aagcctatgc cgcgtcgctc gccggcgtcg
     4021 acgccgccgg gatcaccgat gcggccgcct tcagcgcgct gccgctgacg cgcaagtcgg
     4081 cactgatcga gctgcaaaag gcgcagcgcc cgttcggcgg ttacgcgccg accggtggcg
     4141 ccgaatactc tcacgtgttt gcatccccgg ggccgatcta cgaacccggc tacgcggtag
     4201 ccgatttctg gcgcttcgcg cgctcgctgt atgccgccgg ctttcgccgc ggtgacctgg
     4261 tgcacaacac cttcagttac cacttcacgc cggccgggca gatgctcgac agcggcgcca
     4321 gggcgctcgg ctgcccggtg attccggccg gcaccgggca gaccgagctg caggtgcaga
     4381 ccatcgccga cctgaagcct gctggctatg tcggcacgcc gtcgttcctg aaaatcattc
     4441 tcgacaaggc cgacgagctc ggcagcgatg tcgcctcgat cgacaaggcg ctggtcaccg
     4501 gcgaggcgct gccgccgccg ctgcgcgagt cattcgccgc gcgcggcatc aacgtattgc
     4561 aatgttacgc caccgccgac ctcgggctga tcgcctacga atccgacgcc cgcgaggggc
     4621 tgatcgtcga cgagggcgtc tacctcgaga tcgtgcgtcc gggcagcggc gacccggtcg
     4681 ccgacggcga ggtcggcgag gtcgtggtca ccagtctggg ccgcgaatat ccgctgatcc
     4741 gcttcgccac cggcgacctg tcggctttca tgcccggcgc cagcccctgc gggcgcacca
     4801 accgccgcat caccggctgg atggggcgcg ccgaccagac cgccaaggtg cgcggcatgt
     4861 tcatccatcc gcaacaggtc gacgcggtcg tcaagcggca tcccgagatc gcgcgcgccc
     4921 gcctggtcgt cgactgggta aaccaggccg acgagatcac cctgcaatgc gaagtcgacg
     4981 attacaatga atcgctcggc gcggcgattg ccgacagcat tcgcgcgatc tgcaaattgc
     5041 gcggcgaggt cgatctgcag gcacccggca gcctgcccaa cgacggcaag gtcatcgacg
     5101 acgtccgcaa gtacgactga agccgcgatg acttcccgcc cgctgctcgc cggcgtccgc
     5161 gtcatcgacc tgtcgcaata catccccggc ccgttcgcga cccggcaact cgccgatctc
     5221 ggcgccgacg tgatcaagat cgagccgccc ggcggcgatc cgatgcaaca cttcatggcg
     5281 acggaaaacg atgagccctc gccggtctac cgccatatca atcgcggcaa gcgcatctgt
     5341 cgcgtcgatt tgaaacagga tgacggtcaa cgcatccttg ccgggctgct ggagaatgcc
     5401 gatatcctgc tggagtcctt tcgccccggc gtgctcgcgc gtctcggttt cgaccgcgac
     5461 cggctcgacg cgatcaaccc gcggctgatt cactgcgcac tgtccggctt cggccacacc
     5521 ggtccctacg cgcagcgcgc cggacacgac atcaattact gcgcgctggc cggacaatcg
     5581 ctggtcagcg gcaccgccga gcggccagtc atcggttttc cgccgattgc cgaccatgcg
     5641 tcagcgcagc aggcggcgat cgcgatgctg gccgcgctgc acggccggga gaatcgcgat
     5701 ggcggaattt ttctcgacat cagcatcagc gaatcgattc tcgcctggca gtacctgccg
     5761 gcgctcggtg gcgcgagtga acgcgccgcg tcgatcctcg acggc
//
LOCUS       DATCZV010000205         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_227710, whole genome shotgun sequence.
ACCESSION   DATCZV010000205 DATCZV010000000
VERSION     DATCZV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227710"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /gene="tatC"
                     /locus_tag="VIW27_04620"
     CDS             complement(<1..678)
                     /gene="tatC"
                     /locus_tag="VIW27_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5738983.1"
                     /translation="MSEEEDLEQSKAGSLMSHLVELRDRVVRMVVAILVIFAGLFYWA
                     NDIYIYLAEPLTRHLPENANMIAIDVASPFLTPFKLVLMLSVFLAMPVILYQIWAFVA
                     PGLYANEKRIAGPLLVSSIILFYAGVAFAYYVVFPLVFGFFTRIGPEIVNISTDIGRY
                     LDFVLALFFGFGLAFEVPIATIILVAIGFTTPKKLSEKRPYVIVGAFIIGMMLTPPDV
                     ISQVLLAL"
     gene            complement(675..1139)
                     /gene="tatB"
                     /locus_tag="VIW27_04625"
     CDS             complement(675..1139)
                     /gene="tatB"
                     /locus_tag="VIW27_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY5738984.1"
                     /translation="MFDMGFTEMMLIGIVALIVIGPERLPGVARTAGKYFGRLKRFMT
                     SVRADVEQELRADELRQILAQQQRELDTLKDSISETGKEIGAEAAAVGKVGEELAASL
                     EDDPAAAKPATETATETALTADDGPELLNETPAPAADDAEPVDEASAANSRR"
     gene            complement(1189..1416)
                     /gene="tatA"
                     /locus_tag="VIW27_04630"
     CDS             complement(1189..1416)
                     /gene="tatA"
                     /locus_tag="VIW27_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5738985.1"
                     /translation="MGFGGISIWSLLLILAIVILLFGTKKLRNVGGDLGGAIKNFKKS
                     VKDEEVVQDNPDTSNEGQVIEGKVTEEKDKV"
     gene            complement(1425..1724)
                     /locus_tag="VIW27_04635"
     CDS             complement(1425..1724)
                     /locus_tag="VIW27_04635"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY5738986.1"
                     /translation="MTVLASRKDAEPDSSYVAGLYAKGLDAILKKIGEESAETIIAAK
                     SGDRQQIVHETADLWFHCLVMLAQQNLHARQVLEELERRFGVSGIDEKAAREQDK"
     gene            complement(1748..2131)
                     /gene="hisI"
                     /locus_tag="VIW27_04640"
     CDS             complement(1748..2131)
                     /gene="hisI"
                     /locus_tag="VIW27_04640"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY5738987.1"
                     /translation="MPGPMMEPKFDDRGLVVTVAQDRLTGEVLMVAWMNREALQLTAE
                     QQCAVYWSRSRRKLWFKGEESGHRQRLREIRVDCDADVVLLQVEQVGGIACHTGRRRC
                     FFRVLRDGEWQIDEQIIKNPDEIYR"
BASE COUNT          424 a          688 c          634 g          434 t
ORIGIN      
        1 cagcgccagc agtacctgcg aaatcacgtc gggcggggtc agcatcatgc cgatgatgaa
       61 tgcgccgacg atcacgtagg ggcgtttttc ggacaacttt ttcggtgtcg taaagccgat
      121 ggcgaccaga atgatggtcg cgatcggcac ctcgaacgct agcccgaagc cgaaaaacag
      181 cgccagcacg aaatccaggt aacggccgat gtcggtcgat atgttgacga tttccgggcc
      241 gatacgggtg aagaagccaa ataccagcgg aaacacgacg taataggcaa acgcgacacc
      301 ggcgtagaac aggatgatgc tcgataccag cagcgggccg gcgatgcgct tttcgttagc
      361 gtagagaccg ggcgccacga aggcccagat ctggtagaga atgaccggca tcgccaggaa
      421 tacggacagc atcagcacca gcttgaacgg cgtcaggaac ggcgaggcga cgtcgatcgc
      481 gatcatgttg gcgttttccg gcaggtgccg ggtgagcggt tcggccaggt agatatagat
      541 atcgttggcc cagtagaaca ggccggcgaa tatgaccagg atcgccacca ccatgcgcac
      601 cacgcggtcg cgcagttcga ccaggtgcga catcaggctg ccggctttag actgctccag
      661 gtcctcttct tcgctcatcg gcgcgaattc gcggcgctgg cctcgtcgac gggctcggcg
      721 tcgtcggcgg ccggcgcggg ggtctcgttg agcagttcgg gtccatcgtc cgccgtgagc
      781 gcggtctcgg tggcggtctc ggtggcgggc ttggcggcgg cgggatcgtc ctcgaggctg
      841 gccgccagct cctcgcccac cttgccgaca gccgcggcct ccgcgccgat ctcttttccg
      901 gtctcggaga tgctgtcctt gagggtgtcc agttcgcgtt gctgctgagc caggatctgg
      961 cgcaactcgt cggcgcgcag ctcctgctcg acatcggctc gcacgctggt catgaaccgc
     1021 ttcagacgac cgaaatactt gcccgccgtg cgcgccacgc cgggaagccg ttcggggccg
     1081 atgacgatca aggcgacgat gccgatcagc atcatctcgg tgaaacccat gtcgaacata
     1141 gctttccggc cgctacccga tcaattgcag gtaagagtgt aagctggctt agaccttgtc
     1201 cttctcctcg gtgaccttgc cctcgataac ctgcccttcg ttggaggtat cgggattgtc
     1261 ctggacgact tcctcgtcct tcaccgactt cttgaaattc ttgatcgcgc cacccaggtc
     1321 accgccgaca ttgcgcagct tcttggtacc gaacagcagg atcacgatcg ccaggatcag
     1381 taacagcgac cagatactaa ttccaccaaa acccatttct cacctcattt atcttgttcg
     1441 cgcgcggcct tttcatcgat cccggagacc ccgaaacgcc gttccagttc ctccagaacc
     1501 tgccgtgcat gcaaattctg ttgtgccagc atcaccaggc agtgaaacca caaatcggcg
     1561 gtttcgtgaa caatttgctg cctgtcaccc gatttcgcgg caatgatggt ttccgccgat
     1621 tcctcgccga ttttcttcaa aatcgcgtcc aggcccttag cgtacaggcc cgcgacgtaa
     1681 gacgaatccg gctcggcgtc cttcctcgat gccagcaccg tcatcaggcg caggagtata
     1741 tcatcactca tcgataaatc tcgtcgggat ttttgattat ttgctcgtcg atttgccatt
     1801 ctccgtcgcg caaaacccga aagaaacagc gcctgcggcc ggtatgacag gcgattccac
     1861 cgacttgctc gacctgcagc aggacgacgt cggcatcgca gtcgacacgg atttcccgca
     1921 gtcgctgtcg atggccggat tcctcgccct tgaaccacag cttgcgccgc gagcgcgacc
     1981 agtaaaccgc acactgctgt tccgcggtca gctgcagggc ttcgcgattc atccacgcga
     2041 ccatcagcac ctcgccggtc aggcgatcct gcgccacggt gaccaccaga ccgcggtcgt
     2101 cgaacttcgg ctccatcatc gggccgggca gggtagcagg ctcgccggag ccggcgatcg
     2161 ccccggctgc gggggctgag
//
LOCUS       DATCZV010000206         4958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_279887, whole genome shotgun sequence.
ACCESSION   DATCZV010000206 DATCZV010000000
VERSION     DATCZV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279887"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1302
                     /gene="fabF"
                     /locus_tag="VIW27_04645"
     CDS             64..1302
                     /gene="fabF"
                     /locus_tag="VIW27_04645"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY5738988.1"
                     /translation="MSKRRVVVTGLGLLTPVGNTVEESWKNIVAGKSGIAPITAFDAS
                     QFSTRISGSVKDFDATEYIAAKDLKKMDTFIHFGIAAGIQALDDSGIEVSDENAERIG
                     VAIGSGIGGLPSIEKNRDAYVAGGPRKISPFFVPSSLINMVSGNLSIMRGLKGPNISI
                     VSACTTGAHNIGDAARMISYGDADVMVAGASEMATSALGVGGFAAARALSTRNDDPEA
                     ASRPWDRDRDGFVLGDGAGVVVLEEYEMAKKRGANIYCELIGYGMSGDAYHMTAPSEG
                     GEGAARCMINAMNDAGLNPEDVNYINAHGTSTPAGDIAETNAVKLAMGDHAYKLVVSS
                     TKSMTGHLLGAAGGIEAIFSILALRDQVVPPTINLDNQDPECDLDFVANTAREMKLSV
                     SMSNSFGFGGTNGTCVFKLI"
     gene            1467..2483
                     /gene="mltG"
                     /locus_tag="VIW27_04650"
     CDS             1467..2483
                     /gene="mltG"
                     /locus_tag="VIW27_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY5738989.1"
                     /translation="MKFIKKFIAISMAGAALAIAVLVFKLLSFQHGSVTLPESEQVFL
                     ISPGSNIKSIAQDMTRRGIIDDPWLFILLAKLKDVETRVRAGEYLLEAGQTPDNLLET
                     FTTGKSIQYGFTVIEGWSFRQMMDALAEEPVIMHTLQGKSDAEIMRAIGYPDQHPEGM
                     FFPDTYRFPKGTTDADFLKRAYDVMQQHLEREWLQREPDLPLNSSYEALILASIIEKE
                     TAVGSERPLIAGVFTERLRRNMRLQTDPTIIYGLGSGFDGDIRFRDLKKDTPYNTYLH
                     AGLTPTPIALPGLDSIRAALHPEPTEALYFVSKGDGTHHFSATLEEHNAAVARYQLKR
                     RKKN"
     gene            2498..3163
                     /gene="tmk"
                     /locus_tag="VIW27_04655"
     CDS             2498..3163
                     /gene="tmk"
                     /locus_tag="VIW27_04655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018693214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY5738990.1"
                     /translation="MNDTDNRMIVIDGVDGSGKGVQSRRLLAAFDAAGMPAILTREPG
                     GCPAAEEIRGLLVTGEPDKWDDMTELLLMYAARRAHLRDTVWPALADGLWVISDRYAD
                     SSRAFQGIAGNLGLDRVEAVHRTAIGEFEPALVIILDLPETVALQRTRERGGSEDRFE
                     KKGTAYHARVRNAFLQIAAGNPRRYEVVDAGQSIDRVGDAILDLVNRRFGLQLAAATE
                     AAR"
     gene            3160..4155
                     /locus_tag="VIW27_04660"
     CDS             3160..4155
                     /locus_tag="VIW27_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5738991.1"
                     /translation="MSARPTQYLPWQAAVVEHALALKAGGRLPQAVLLEDASQRDIGG
                     FVDYLCRLLLCDQPDGLLPCDQCDACRMMRAGTYSDYRFVTLESDEKSKKLSKNIKIE
                     QIRKLIHELSLSHQYARLKIAAIYPAESMNRNSANALLKTLEEPAADVLLLLVTHNRG
                     RIPITLRSRCQSWRIPLPAKPAAREWLQQQGLSTDDAALYLDYADGDPRLALDLQQHG
                     YASLVSEFKQRLGHFLRGSLGVSALCAQLMVYDTQLLRRLVGMTLNAYCYRSSGVDIA
                     GNAAEGADRQRARQLLELRNRAQKQLQVEENNLDLRIQLEDVLISLKQILIRRTV"
     gene            4155..4502
                     /locus_tag="VIW27_04665"
     CDS             4155..4502
                     /locus_tag="VIW27_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5738992.1"
                     /translation="MAAPGSQGILSLNIKDKSALYAAYMPYVKNGGLFIPTNKHYALG
                     DEVFMLLSLMDDKERLPVAGKIIWVTPQGAQSNKAAGIGVQFSAQDHGTTRNKIEGYL
                     AGALQADRPTHTM"
     gene            4620..>4958
                     /locus_tag="VIW27_04670"
     CDS             4620..>4958
                     /locus_tag="VIW27_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5738993.1"
                     /translation="MYFDSHCHLDRIDLDDHAGDFGQLLSTIESSGVERMVCIGVTLE
                     SFEDMYARIAELPQVYCTAGVHPDYENIEEPTVERLCAFAARDKVVAIGETGLDYFHQ
                     SGHLDWQRRRF"
BASE COUNT         1016 a         1526 c         1502 g          914 t
ORIGIN      
        1 agcatgctaa gcggcatttt cattttgcgg ccgcgctgca agtatcgtga aggggagtga
       61 atcttgtcaa aacgtcgtgt tgtagtaacg ggactcggat tgctgacacc ggtcggcaat
      121 accgtggaag agtcctggaa gaacatcgtt gccggaaaga gcggtatcgc gccaatcacc
      181 gcgttcgatg ccagccagtt ttccacccgt atctcgggtt cggtcaagga cttcgatgcc
      241 accgagtaca tcgccgccaa ggatctgaag aaaatggata cgttcatcca tttcgggatc
      301 gccgccggca tccaggcgct tgacgattcg ggaatcgaag tcagcgatga aaacgccgag
      361 cgtatcggtg tcgccatcgg ctccggaatc ggcggcctgc cgtccatcga gaagaatcgc
      421 gacgcctatg tcgccggcgg tccgcgcaag atttcgccgt tcttcgtgcc gagctcgctg
      481 atcaacatgg tgtctggcaa cctgtcgatc atgcgcggcc tcaagggtcc gaatatctcc
      541 atcgtcagcg cctgcaccac cggtgcgcac aatatcggcg acgcggcacg catgatcagc
      601 tacggcgacg ccgacgtgat ggtcgccggc gcctccgaaa tggcgacctc ggcgctcggc
      661 gttggcggct ttgccgcggc gcgcgcactg tcgacccgca acgatgatcc ggaagccgcc
      721 agccgtccct gggaccgcga ccgcgatggc ttcgtgctcg gcgacggggc cggcgtcgtt
      781 gtcctcgagg aatacgaaat ggcgaaaaag cgcggcgcca acatttactg cgaactgatc
      841 ggctacggca tgagcggcga cgcttaccac atgaccgcac cgtctgaagg cggggaaggg
      901 gccgcgcgtt gcatgatcaa cgcgatgaac gatgccggcc tgaatcccga ggacgtcaat
      961 tacatcaatg ctcacggcac ctcgacaccc gctggcgaca tcgccgagac caacgcggtc
     1021 aagctggcga tgggtgacca tgcctacaag ctggtggtca gctcgaccaa gtcgatgacc
     1081 ggccacctgc tgggtgcggc gggcggcatc gaagccatct tttcgatact cgcattgcgt
     1141 gaccaggtcg tgccgccgac gatcaatctc gacaaccagg atcctgaatg cgacctggat
     1201 ttcgtcgcca acacggcacg cgaaatgaag ctttcggtca gcatgtcgaa ttccttcggt
     1261 ttcggcggta ccaacggcac ctgtgttttc aagttgatct agcctcggga tacgcgccgg
     1321 ggaccggacg gcgcctcggc caggctgagc aatgctttgt gacacactgt ttttgcgccg
     1381 cctcgatttc ccgctatgct tctagtaacg acggctcccg gcatcagtgc cggcgagcca
     1441 gccgaacggg ttgacgacga gcgacagtga aattcatcaa gaaatttatc gcgatttcga
     1501 tggccggcgc cgcgctggct atcgccgtgc tggttttcaa gctgctgagt tttcagcatg
     1561 gcagcgtcac cctgccggag tccgagcagg tctttctgat cagtccgggc agtaatatca
     1621 aatcgatcgc ccaggatatg acgcggcgtg gaatcatcga cgatccatgg ctgttcatcc
     1681 tgctggcgaa actcaaggac gtcgagaccc gtgtgcgcgc gggggagtac ctgctcgagg
     1741 ccgggcaaac gcccgacaat ctactcgaga ccttcaccac cggcaagtcg atacagtatg
     1801 ggttcacggt catcgagggc tggtcttttc ggcagatgat ggatgcgctc gccgaggaac
     1861 cggtgatcat gcataccctg cagggcaaga gcgacgcgga gatcatgcgc gccatcggct
     1921 acccggacca gcatcccgag ggcatgtttt tccccgatac ctaccgcttt ccgaaaggca
     1981 ccaccgatgc cgatttcctg aagcgcgcct acgacgtcat gcagcagcac ctggaacgcg
     2041 agtggctgca gcgcgaaccc gacctgccgc tgaattcgag ctacgaggcg ctgatcctgg
     2101 catcgatcat tgaaaaggaa accgcggtcg gctcggaacg gccgctgatc gccggcgtgt
     2161 tcaccgagcg cctgcggcgc aacatgcgcc tgcaaaccga cccgaccatc atctacggtc
     2221 tcggcagcgg tttcgacggc gatatccgct ttcgagattt gaaaaaggat acgccgtata
     2281 atacctacct gcacgcgggg ctgacgccga ctccgatcgc gttgccgggc ctggattcga
     2341 ttcgcgcggc actgcacccc gagccgaccg aggccctgta tttcgtttcc aagggggacg
     2401 gcacgcatca tttctcggcg accctggagg aacataatgc ggccgtggcg cgctaccagt
     2461 tgaagcgacg caagaaaaat tgaacgcgaa tgacaaggtg aacgacaccg ataaccgtat
     2521 gatcgtcatc gatggtgtcg acggctctgg caagggtgtg cagtcgcgcc gcctgctggc
     2581 cgcattcgac gccgccggca tgccggcgat cctgacccgc gaaccgggcg gttgtccggc
     2641 tgccgaggaa atccgcggcc tgctggtaac cggcgaaccg gacaagtggg acgatatgac
     2701 cgaactactg ttgatgtacg cggcgcggcg cgcgcacctg cgcgataccg tgtggccggc
     2761 gctggccgat ggcctgtggg taatcagcga tcgttacgcc gattccagcc gcgcctttca
     2821 ggggattgcc gggaacctgg gactcgatcg cgtcgaagcc gtgcatcgta ccgccatcgg
     2881 cgaattcgaa ccggcactgg tgatcattct cgacctgccg gaaaccgtcg ccctgcagcg
     2941 aacccgcgaa cgcggcggca gcgaggaccg cttcgagaaa aagggtaccg cctaccacgc
     3001 gcgggtacgc aatgccttcc tgcagatcgc tgccggcaat ccacgtcggt acgaggtcgt
     3061 cgacgccggg cagtccatcg accgcgtcgg cgatgcgatc ctcgacctcg tcaatcggcg
     3121 cttcggcctg cagctggcgg ccgcgaccga ggcagccagg tgagtgcgcg gccgacgcaa
     3181 tacctgccgt ggcaggcagc ggtcgtcgaa cacgcactgg cattgaaggc aggcggacgc
     3241 ctgccgcagg ccgtattgct cgaggacgca agccagcggg atatcggtgg tttcgtggat
     3301 tacctgtgcc gactgttgct ctgcgatcag cctgacggcc tgctgccctg cgaccagtgc
     3361 gacgcttgcc gaatgatgcg ggccgggacc tactcggact atcggttcgt tactctcgaa
     3421 tccgacgaaa aatccaaaaa attaagcaaa aacataaaga tagagcaaat acgtaaactg
     3481 atacatgaat tatctttgag ccatcaatac gcgcgcctga agattgccgc gatctatccc
     3541 gcggaatcga tgaaccgcaa cagcgccaat gccttgctga aaaccctcga ggagccggcg
     3601 gccgatgtac tgttgctgct ggtcacccac aatcgcggcc gcataccgat cacgctgcgc
     3661 agccgttgcc agtcctggcg tatcccgctg ccagcgaaac ctgccgcgcg cgaatggctg
     3721 caacagcagg gcctgagcac cgacgatgcc gcactgtatc tcgattatgc cgacggcgat
     3781 ccgcggctgg cgctcgatct gcagcaacac ggctacgcct cgctggttag cgagttcaag
     3841 cagcggctgg gtcatttcct gcgcggcagc ctcggcgtat cggcactttg cgcgcagttg
     3901 atggtttacg atacgcaact gttacggcgc ctggtcggca tgaccctgaa cgcctactgt
     3961 taccgtagca gcggtgtcga catcgccggc aatgccgccg agggcgccga tcggcaacgc
     4021 gcccggcaat tgctcgaact gagaaatcgc gcacaaaagc aattgcaggt cgaagaaaac
     4081 aatctcgatt tacgaattca actcgaagac gtgctaatat ccctgaaaca gattctgatc
     4141 cggagaacgg tctgatggca gcacctggaa gccagggcat actgtcgctc aacatcaagg
     4201 acaagagtgc gctgtacgcg gcttacatgc cctatgtcaa gaatggcggt cttttcattc
     4261 cgaccaacaa gcattacgcg ctcggcgacg aagtgttcat gctgctgagc ctgatggacg
     4321 acaaggaacg cctgccggtt gccggcaaga tcatctgggt gacgccgcag ggcgcgcaaa
     4381 gcaacaaggc cgccggcatc ggcgtgcagt tcagcgccca ggaccatggc accacgcgca
     4441 acaagatcga gggttacctg gccggcgcgt tgcaggccga ccgtccgacg cataccatgt
     4501 aaaatcgccc acgcgcgggc agcggctgcg gtcgcggccg gtggccgctc gactcggtgg
     4561 tccgcgggca tgccggcacc gaggatccgg accgattcac cgttagcagg accccatccg
     4621 tgtatttcga ttcccattgc catctcgacc gcatcgatct cgatgaccac gctggcgact
     4681 tcggccagtt gctgagtacc atcgaaagca gcggcgtcga gcgcatggtc tgcatcggcg
     4741 tgacgctgga gtccttcgaa gacatgtatg cccgcattgc cgagctgcca caggtgtatt
     4801 gcacggcggg tgttcacccc gactacgaaa atatcgagga accgactgtc gagcgcctgt
     4861 gcgcattcgc ggcgcgcgac aaggtggtcg ccatcggcga gaccgggctc gattatttcc
     4921 accagtctgg ccatctcgac tggcagcgcc ggcgcttc
//
LOCUS       DATCZV010000207         4323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_385946, whole genome shotgun sequence.
ACCESSION   DATCZV010000207 DATCZV010000000
VERSION     DATCZV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385946"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VIW27_04675"
     CDS             complement(<1..270)
                     /locus_tag="VIW27_04675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY5738994.1"
                     /translation="MAYILAVTWTAKPGNEDEVLGLLRRLREASNQEPGVITYEAHVD
                     PENPRQFFIYEVYHDASGLEAHQETPHFRELVLERAIPLLEFRERK"
     gene            650..1828
                     /locus_tag="VIW27_04680"
     CDS             650..1828
                     /locus_tag="VIW27_04680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5738995.1"
                     /translation="MTLLATLAALGWWLGIPLRAAVNDRKLQSLADFDYDDSQHWPRI
                     SVLVPARNEADTLEAAMQSLLRVDYPNLEIIIVDDRSSDGTSAIIERLAVADSRIRSM
                     RIDRLADGWLGKVHALHRGLEVCSGEWVLFTDADVHFSRCLLKRALASCLEGSRDFLA
                     LLPEFFATRPLLGAAQLAFATLLLAALDRERIAAPRNPAAMGIGAFNLARKSFLDTRE
                     GLEWLRMEVADDAGLALLMKRRGAAIDIQSGRGLLRVDWYPSLAAMMDGVMQRMVLGA
                     NYRLPVFSLQCLLVCLCLLAPLILALSLLPHSPLAWLSVTLYLLPTIILRTATRHFTP
                     PPAAAWGMPLGFAVIGFGMLRSLLFCLRHGGIYWRGNIYPLADLRAGQRVTVSVFLRR
                     "
     gene            2511..3035
                     /locus_tag="VIW27_04685"
     CDS             2511..3035
                     /locus_tag="VIW27_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HEY5738996.1"
                     /translation="MSEESEVSRARLALEKDLFLRTLIRELSGTLEDVVGLDEAAGFI
                     SIVGQKIGDWIDAEYRQALHTEKLDGQQVRDALIDLKTRIDGDFYLLAEDDEKIVLGN
                     RRCPFGDKVLDRPSMCMMTSNVFGTITAENLGYAKVSLEETIARGSRECRVVVYKKQG
                     TESDTADGRVYYAS"
     gene            3051..>4323
                     /locus_tag="VIW27_04690"
     CDS             3051..>4323
                     /locus_tag="VIW27_04690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5738997.1"
                     /translation="MTPNRFLQIVDAIPESFMLIDSEGRILSVNRHAAEFLGIDRHHS
                     GANIGDLVSTPSEKLEAWLRIWSRSQAPVPFRLAWKNLDPETQSRLLCQAFVLEPGTR
                     NRLARIVLRCVAGRSVASRFVELNREIDRQKTVLEKLKHSREALEAEIERAKVTLRSI
                     GDAVITTDKRGRIDFLNPVAEKLTGWLNTDATGRPVAEVFRIVNELTREPADDPVEYC
                     LLRERTVGLAHNTVLISRDGTDYVIEDSAAPIRNNDGEIVGVVLVFRDVTGERLAHRQ
                     LEYLAQHDTLTGLKNRFYFEQQLGRVVDVAARGRHGCALLYLDLDRFKLVNDTAGHAA
                     GDQLLIEIAAMLAERLRQGDVLARLGGDEFGIILDNVRDDQLYDIAGKFSRALSNSLF
                     QWEGNDYNITCSVGVAMIDERTLSSAEAMRQA"
BASE COUNT          829 a         1332 c         1310 g          852 t
ORIGIN      
        1 cttgcgttcg cggaattcga gcagcgggat cgccctttcc agcaccagct ccctgaaatg
       61 cggagtttcc tggtgcgctt ccagtccgct ggcgtcgtga tagacctcgt aaatgaagaa
      121 ttgccgcggg ttttccgggt cgacatgcgc ttcgtaggtg attacgccgg gttcctggtt
      181 cgacgcttcc cgcagccgtc gcagtagccc gagtacctcg tcttcgttgc ccggtttcgc
      241 ggtccaggta accgccagta tatatgccat attcccctct ccccgctggc aatggtttgc
      301 aatggtggcg tcgatattaa cagccaggcg cgaaactttc tcgcccgggc ggcacaaatc
      361 cggcgcactc gctgctggcg atgcccgaag atcgccgtcg ggcgcactcg ccgccgaccg
      421 tggcgagttt gtttacgccg cggcaccccc tatactcgag cagcggtccg ccggcattgc
      481 cgcagcggtt cgaccacacc gggaaacccg ccggacccgg ccaaccaaaa tatcgagaga
      541 cagaacgccg tgcaactccg gattcagtgc gcaactagcg gcgcaactag cggctcgacg
      601 cccggcgtca agatacgcgg gaccggcgcg aatggtggat aactggttag tgacactgct
      661 agcgacattg gccgcgctcg gctggtggct ggggatacca ctgcgcgcgg cagtcaacga
      721 ccgcaagctg caatcgctgg ccgacttcga ctacgatgat tcacagcatt ggccgcggat
      781 cagcgtactg gtacccgcca ggaatgaggc ggacacgctc gaggcggcca tgcaaagcct
      841 gctgcgtgtc gattatccga atctcgaaat cattatcgtc gacgaccgtt cgagcgatgg
      901 caccagtgcc atcatcgagc ggctcgcagt cgccgattca cggattcgca gtatgcgcat
      961 cgatagactt gccgatggct ggctcggcaa ggtgcatgct ctgcaccgcg ggctcgaggt
     1021 ctgcagcggc gaatgggtat tgttcaccga tgccgacgtc catttttccc gctgtctgct
     1081 gaaaagagcg ctcgccagtt gcctcgaggg cagcagggat tttctcgccc tgctgccgga
     1141 atttttcgct acccgcccgt tgcttggcgc cgcgcaactg gccttcgcga ccctcttgct
     1201 ggcggcgctc gatcgcgagc ggatcgccgc gccacggaat ccggcggcga tgggtatcgg
     1261 cgccttcaac ctggcgcgca aaagcttcct cgatacgcgc gaggggctgg agtggctgcg
     1321 catggaagtc gccgatgacg ccggcctggc cttgctgatg aagcgccgcg gcgcggcgat
     1381 cgacattcaa tcgggccgcg gactactcag ggtcgactgg tacccgtcgc tggcggcgat
     1441 gatggacggc gtcatgcagc gcatggtgct gggggccaat taccgccttc ccgtgttttc
     1501 gctacagtgc ctgctggtgt gcctgtgcct gttggcgccg ctcatactgg cgctgtcgct
     1561 gctgccgcat tcgccgctgg cctggctgag cgtcacgctt tacctgctgc cgacgatcat
     1621 tctgcgcacc gcgacccggc atttcacgcc gccacccgcg gctgcctggg gaatgccgct
     1681 cggatttgcc gtcatcggct tcggcatgct gcgttcactg ctgttctgtc ttcgccatgg
     1741 cggtatctac tggcgcggca acatttatcc gctggccgat ctgcgcgccg ggcagcgcgt
     1801 cacggtcagc gtttttttgc ggcgatgatc ctgtcagggc gcagacaggc aacagcgccc
     1861 ggcaaacccc ggcgaccacc gggatcgcct gcccgcagcc atactgccac ctggcagaat
     1921 cgccgacagc ccgacggtta atggatccgg ccgacacggc ccggctaaat tcgctgatcg
     1981 accgcgctgc cgtcgcgcac cgtcggcgtg cgccggcgag cgataatttg gtgcatggac
     2041 cacccgacta atcgacgccg taaaaccgtt cactggtaag cctatgaaat ttagagattt
     2101 aatttatcgc tttatttcgt ccgccgacag ccaaccggag tcggcgcaga attgtgatat
     2161 gttttgtgca tcgcagcatc gggtcccgga ctcgccgacg actgccggct tcacccgggg
     2221 cccgcaattt aactgcccat ggtttcgggt cagcatggct ggcgccgatc gtcccaagat
     2281 cttctcctcc taagatattg ggatattcag gaccgcttaa aaccggtcct gttttttatc
     2341 agcgtcgcgc acggcaataa gagccgcgat gactcgccgg cctgcagatg ccgcggtacc
     2401 aatatgaaca gggtcgtcta tactttctga catccatcca ttgcgagaat tattcgggga
     2461 attctatcgg tggccccggt tcccgcgcag gcaatgcata aggaatggtc atgtcagaag
     2521 aaagcgaggt ttcccgggcg agattggcac tcgaaaaaga cctcttcctg cgcactctga
     2581 tacgcgaact ctcgggcacg ctggaagatg tcgtcggcct cgacgaagcc gccggcttca
     2641 tcagtatcgt gggccaaaag atcggcgact ggatcgacgc cgagtatcgc caggccctgc
     2701 acacggaaaa gctggacggt cagcaggtaa gagacgctct tatcgatctg aaaacgcgca
     2761 tcgacggcga tttttacctg ctcgcggagg atgacgaaaa gattgtcctc ggcaatcggc
     2821 gctgcccgtt cggcgacaag gtcctcgacc ggccctcgat gtgtatgatg acttcgaacg
     2881 tattcggcac gatcaccgcc gagaacctcg gatacgccaa ggtcagcctc gaggagacca
     2941 tcgcccgtgg ctcgcgggag tgcagggtag tcgtctacaa aaagcagggc acggagtcag
     3001 acacggcgga cggccgggtt tattacgcta gctgagctgg aacttctccg atgacgccga
     3061 accggtttct gcagatcgtc gatgcgatcc ccgaatcatt catgctcatc gacagcgagg
     3121 gcaggatcct gtcggtcaac cgccatgcag ccgaatttct cggtatcgat cgccaccaca
     3181 gcggcgccaa tatcggcgac ctggtctcca cgccatcgga aaagctcgag gcttggttac
     3241 gcatctggag ccgcagccag gcaccggttc cgttccgcct ggcatggaag aacctcgatc
     3301 ccgagactca atccaggctg ctgtgccagg ctttcgtact cgagcccggc actcgtaatc
     3361 gcctggccag gatcgtgctg cgctgcgtgg ccggcaggtc ggttgcgagc agattcgtcg
     3421 agctgaatcg ggagatcgac cgccagaaga cggtgctgga gaaactcaag cacagccgcg
     3481 aggcgctcga ggcggagatc gaaagagcaa aggtaaccct gcgatcgatc ggcgatgcgg
     3541 taatcacgac cgacaaaagg ggccgtatcg actttctgaa cccggttgcc gaaaagctga
     3601 ccggctggct caatacggac gccacgggcc gccctgtcgc cgaggtattc aggatcgtca
     3661 atgaattgac ccgcgaaccg gccgacgacc cggtcgagta ctgcctgctt cgagaacgaa
     3721 ctgtcggcct tgcgcacaac accgtcctga tatccaggga cggtaccgac tacgtaatcg
     3781 aggattcggc ggcgccgatt cgcaacaacg atggagagat cgtcggcgtg gtgctggtat
     3841 ttcgcgatgt caccggtgag cgactggcgc accggcaact cgagtatctc gcccagcacg
     3901 atacgctaac cggcttgaag aatcgctttt atttcgagca acaactgggg cgcgtcgtcg
     3961 acgtcgccgc ccgcggcagg cacggctgcg ccctgcttta cctggacctg gaccgtttca
     4021 agctggttaa cgataccgcc gggcacgcgg ccggcgacca gttgctgatc gagatcgccg
     4081 cgatgctagc cgaacgattg cgtcagggcg atgtgctggc caggctcggt ggtgacgagt
     4141 tcggtatcat cctcgacaac gtcagggatg accagctgta cgatatcgcc ggcaagtttt
     4201 cgcgtgcgct gagcaactcg ctgtttcaat gggaaggcaa cgactacaac atcacctgca
     4261 gtgtcggggt agcgatgatc gacgaacgca cgctatcatc ggcggaagcg atgcgccagg
     4321 ccg
//
LOCUS       DATCZV010000208         5053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_756581, whole genome shotgun sequence.
ACCESSION   DATCZV010000208 DATCZV010000000
VERSION     DATCZV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756581"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1055)
                     /locus_tag="VIW27_04695"
     CDS             complement(69..1055)
                     /locus_tag="VIW27_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY5738998.1"
                     /translation="MKHWQQFEARRGAARLSENFVCDADGLGLILVTGDDARDFLQNQ
                     LSNDIGLIDETRLQLSSYSTPKGRMVGIFRVIQVSNGYLLLTVKSMVQPLLERLFHFI
                     VKSRVQLAAASDYFARIALVTDNPQVLQHQGLPREPGEVLQAESVIAMQLEPLGSQRR
                     FVIMCLNGAGAVELWEQFTTQLQAADFESWRLSEIRAGIPSIYPPTSEQFVLQMTNLG
                     VLGGVSFKKGCYPGQEIVARMQYLGKLKRRMFLAELETEQLPLAGDELVLAGKLEADG
                     SGTVVDAEFDADGVAHCLYVAQIARAESGSLQLLRQPDTRIDNVDLPYPPPA"
     gene            complement(1052..2104)
                     /gene="queG"
                     /locus_tag="VIW27_04700"
     CDS             complement(1052..2104)
                     /gene="queG"
                     /locus_tag="VIW27_04700"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY5738999.1"
                     /translation="MSITSTIKQLGLELGFQQVGITDVELDSEHRRYQQWIAHNYHGE
                     MAYMARNIDKRLHPPRLVPNTLSVICVRLDYLVEPQQRMLELLDHPRQAYVSRYALGR
                     DYHKLMRKRLQKYADRIIEICGPMGYRVFTDSAPVLEKALAARAGLGWQGKHSNLLQR
                     EHGSWFFLGEIYTDLELETDAPLENHCGRCTSCIDICPTDAIVEPYTVDARRCISYLT
                     IEHRSEIPLEFRAAIGNRIYGCDDCQLACPWNRFAQFTREPDFQPRHGLDSISLLDCY
                     RWSEDEFLRNTEGSAIRRIGYQCWLRNIVVALGNAAPDPHIVATLKRDYDAHSGFVQL
                     HIDWAIDRQQRQEEQR"
     gene            2203..2580
                     /gene="crcB"
                     /locus_tag="VIW27_04705"
     CDS             2203..2580
                     /gene="crcB"
                     /locus_tag="VIW27_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY5739000.1"
                     /translation="MSIYLAIAAGGSLGAMSRYWVSMTTYQWLGQHFPHGTLLVNLLG
                     SLAMGFLSVLLVHRFQVSDEIRFGLLAGFLGSFTTFSTFAMDTLQLASSEALFKAALY
                     VLLSVALCILGAWAGLVAAKQMI"
     gene            2584..3864
                     /gene="serS"
                     /locus_tag="VIW27_04710"
     CDS             2584..3864
                     /gene="serS"
                     /locus_tag="VIW27_04710"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY5739001.1"
                     /translation="MLDPKILRSDLDLVAEKLLVKKYELDKARFTDLEESRKKLQVET
                     QNLQNERNKLSKAIGQAKSRGEDAEPLLRDVESRSADLKSAETRLAEIQGDLDYLLLR
                     IPNLPDESVPAGDSEDDNTEVLRWGEPRQFDFEPRDHIEIGRLLGGMDFERAAKLSGA
                     RFVTMTGDLVTLQRALIQFMIDRHITEHGYTEAYAPYLVNEATLTGTGQLPKFKEDLF
                     HIVSEDHDLYLIPTAEVPVTNFVADTIVEAEQLPLKFVAHTPCFRSEAGSYGKDTRGM
                     IRQHQFEKVELVQIVRPADSMAALEELTGHAESILRALELPYRKVILCGGDLGFASTK
                     TYDLEVWLPGQQTYREISSCSNMTDFQARRMKARWRNPASGKPEAVHTLNGSGLAVGR
                     TLIAIMENFQQSDGSIRIPEALQPYLRGKTEIGI"
     gene            3951..4409
                     /locus_tag="VIW27_04715"
     CDS             3951..4409
                     /locus_tag="VIW27_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5739002.1"
                     /translation="MLLPFTILRKDDGTTMLSFSDFKLPDFSPPALPELPKVDSITDS
                     VGTSGGQVTIYEWRDSEGNIQFTNEPPPQGVEFAIRQFDPNANVIQSVQLPTEEPEPA
                     ASETQQPQAPGAVEAPSPYSPESVKKLMDDAKNVEKLLNQRFQNQQTAID"
     gene            4426..4914
                     /locus_tag="VIW27_04720"
     CDS             4426..4914
                     /locus_tag="VIW27_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY5739003.1"
                     /translation="MKLTTLILLAMMSCSLQAAGKFAFINSKMLLEQSPQAIAANSAL
                     QEQFGERESTLRNLAQSIQEMEQTYQTDRAIMSDEQKQKAEDNILQSKRRFQFEQQSL
                     KEDVQAKQRELLQQVQTEIKAAIQAYGESQGYDFIFTDTSIAFASDAVDITDEILQEL
                     KK"
BASE COUNT         1039 a         1488 c         1539 g          987 t
ORIGIN      
        1 atatcggcct cgactacggc gaccccgggg ccatgctgcg gcaaatcgaa gctgcctgac
       61 gcgctgtctc aggcaggcgg cgggtagggc agatcgacgt tgtcgatacg ggtatcgggc
      121 tgcctcagca actgcagcga gccgctctcg gcgcgagcga tctgtgcgac atagaggcag
      181 tgggcgacgc cgtcggcgtc gaactcggca tcgaccacgg tcccgctgcc gtcggcctcg
      241 agcttgccgg ccagtaccag ttcatcgccc gccagcggca gctgctcggt ttcgagttcg
      301 gcgagaaaca tgcgccgttt gagtttgccg aggtattgca tgcgggcgac gatttcctgc
      361 ccgggatagc aacccttctt gaaactgacc ccgccgagga cgcccaggtt ggtcatctgc
      421 aaaacaaact gttcgctggt cggcggataa atcgacggga ttccggcgcg gatttcggac
      481 agccgccagg attcgaaatc ggcggcctgg agttgcgtgg tgaattgttc ccacagttcg
      541 acggcgccgg cgccgttcag acacatgatc acgaacctgc gttgcgagcc gagcggttcg
      601 agctgcatcg cgatcaccga ttcggcttgc agcacctcgc ccggttcgcg cggcaagccc
      661 tggtgttgca acacctgtgg attatcggtc accagtgcga tgcgcgcgaa gtaatcgctg
      721 gcagcggcga gctgcacccg cgatttgacg atgaaatgaa acaggcgttc gagtaacggc
      781 tgtaccatcg atttcacggt cagcaacagg tagccgttgg atacctggat taccctgaag
      841 ataccgacca tgcgaccctt gggcgtcgag taggagctga gctgcagccg cgtttcgtcg
      901 atcagtccga tatcgttgct gagctggttt tgcaggaaat cgcgcgcgtc gtcgccggtg
      961 accagtatca gtccgagccc gtcggcgtcg cagacgaagt tttcgcttag ccgcgccgcg
     1021 ccgcgccgcg cctcgaattg ctgccagtgt ttcatcgctg ttcttcctgt cgttgctgtc
     1081 ggtcgatggc ccagtcgata tggagttgta cgaagccgct gtgcgcgtcg tagtcccgtt
     1141 tcagtgtcgc tactatatgc gggtcaggcg ccgcgtttcc aagcgcaacg acgatattcc
     1201 tgagccagca ttggtaaccg atgcggcgaa tcgccgaacc ctcggtgttg cgcagaaact
     1261 cgtcttcgct ccagcggtaa cagtccagca gggaaatgct gtcgagcccg tgtcgcggct
     1321 gaaaatccgg ctcgcgggtg aattgggcga agcggttcca cgggcacgcc agctggcaat
     1381 cgtcgcagcc gtaaatgcgg ttgccgatgg cggcgcgaaa ctcgagcgga atctccgagc
     1441 ggtgctcgat cgtcaggtag gaaatacagc gccgcgcgtc gaccgtgtag ggctcgacga
     1501 tggcgtcggt cggacagata tcgatgcagc tggtgcagcg cccgcagtgg ttttccagcg
     1561 gcgcatcggt ttcgagctcc aggtcggtgt agatctcgcc gagaaaaaac cacgagccat
     1621 gttcgcgctg cagcaggttg ctgtgcttac cctgccagcc gagtccggcc ctggcggcga
     1681 gggccttttc cagcactggc gcgctgtcgg tgaatacccg gtaacccatc gggccgcaga
     1741 tttcgatgat gcggtcggcg tatttctgca gacgcttgcg catcagcttg tgataatcgc
     1801 ggccgagcgc gtagcgcgac acgtaggcct gccgagggtg gtcgagcaat tccagcatgc
     1861 gctgctgcgg ttccaccagg taatcgagcc gcacgcagat caccgacagg gtattcggca
     1921 ccagccgcgg tgggtgcagg cgcttgtcga tgttacgcgc catgtatgcc atctcgccgt
     1981 gatagttatg cgcgatccac tgctggtaac gccggtgttc ggaatcgagc tcgacatcgg
     2041 tgatgccgac ctgttgaaag cccagttcga ggccgagttg cttgatggtg ctggtgatcg
     2101 acaaggaatt agtctgcctg gtctggtgtg cttatttggc gctgattcga gtgcatcttt
     2161 gcgggcggct ggtacaatat cgcattttta accaaatctg aaatgtcgat ctatttagcc
     2221 atcgccgcgg gcggttcgct gggcgcgatg tcccgttact gggtatcgat gaccacttac
     2281 cagtggctgg gtcagcattt cccgcacggc acgttgctgg tcaacctgct cggatcgctg
     2341 gcgatgggat tcctgtccgt attgctggtg caccgttttc aggtcagtga cgaaatccgc
     2401 ttcgggctgc tggccggttt tctcggctcg tttacgacat tttcgacttt cgcgatggac
     2461 acgctgcagc tggcctcgag cgaagccctg ttcaaggccg cgctctacgt cctgctcagc
     2521 gtggccctgt gtatcctcgg cgcctgggca ggcctggtcg ccgccaaaca gatgatatga
     2581 ccgatgcttg atccgaaaat tttgcgcagc gatctcgacc tggtggccga gaaacttctg
     2641 gtgaagaaat atgaactcga taaggccagg ttcaccgatc tggaagaaag ccgcaagaag
     2701 ctgcaggtag aaacccagaa tctgcaaaac gaacgcaaca aactctcaaa ggccatcggt
     2761 caggcgaaaa gcaggggaga ggatgcggag ccgttgctca gggacgtgga aagccgcagt
     2821 gccgacctca agagcgcgga gacccggctg gcggagattc agggcgacct ggattacctg
     2881 ttgctgcgca ttcccaatct gcccgacgaa tcggtgcccg ccggcgacag cgaagacgac
     2941 aacaccgagg tattgcgctg gggcgaaccg cggcagttcg atttcgagcc ccgcgaccac
     3001 atcgaaatcg ggcggctgct cggtggcatg gatttcgaac gcgcggcgaa gctgtccggg
     3061 gcgcgtttcg tgaccatgac cggcgacctg gtgacgctgc agcgtgcatt gatccagttc
     3121 atgatcgacc gccatatcac cgagcacggt tacaccgagg cttacgcgcc ctatctggtc
     3181 aacgaggcca cgctgacggg gaccggccaa ttgcccaagt tcaaggaaga cctgttccac
     3241 atcgtttccg aagaccacga tctctacctc attccgactg ccgaggtacc ggtgaccaat
     3301 ttcgtcgccg acaccatcgt cgaggccgag cagttaccgc tcaagttcgt cgcccacacc
     3361 ccctgtttcc gctccgaggc cggctcctac ggcaaggaca cccgcggcat gattcgccag
     3421 catcagtttg aaaaggtcga gctggtgcaa atcgtcaggc cggcggattc gatggccgcg
     3481 ctcgaggaat tgaccggtca tgccgaaagt attctgcgcg ctctcgagct gccttaccgc
     3541 aaggtcatcc tctgcggcgg ggatctcggc ttcgcgtcga ccaagaccta cgacctcgag
     3601 gtctggctgc cgggtcagca gacctaccgg gaaatttctt cctgcagcaa catgaccgac
     3661 ttccaggcgc ggcgcatgaa agcacgctgg cgcaatccgg caagcggcaa gccggaagcg
     3721 gtgcatacgc tgaatggttc ggggctcgcg gtcgggcgca ctctgatcgc gatcatggaa
     3781 aacttccagc agagcgatgg cagcattcgt attcccgagg cactgcagcc ctacctgcgc
     3841 ggcaagactg aaatcgggat ttgacagtct atactgaggg tgagctgcgc aaacccggaa
     3901 acctgatgaa attattcgcc aaactgttac tcgcggcact ggtcgtcgcc atgctgctgc
     3961 cgtttacgat cctcagaaaa gacgatggca ccacgatgct gagtttttcc gacttcaagc
     4021 tacccgattt ttcgccgccg gcgttgcctg aattgcccaa ggtcgatagc attaccgatt
     4081 ccgtcggcac gagcggcggc caggtgacga tttacgagtg gcgcgacagc gagggcaaca
     4141 tccagtttac caacgaacca cccccgcaag gggtcgaatt cgcgattcga cagttcgatc
     4201 ccaatgctaa tgtgatacag tcggtgcaac tgccgaccga ggagccggaa ccggcagcta
     4261 gtgaaacgca gcagccgcaa gcacccggcg ccgtggaggc acccagcccg tattcgccgg
     4321 agagcgtgaa aaaactgatg gatgacgcca agaacgtcga aaagctgctc aaccaacgtt
     4381 tccagaacca acaaaccgca attgactgaa aaggatgacc gaggcatgaa attaaccacc
     4441 ctgatcctgc tggcgatgat gagttgctcg ctgcaggccg ccggcaagtt cgcttttatc
     4501 aattcgaaga tgctgctcga gcagtcgccg caggcgatcg ccgccaatag cgcgttgcag
     4561 gagcagttcg gcgagcgcga atcaaccctg cgcaacctgg cccagagcat ccaggagatg
     4621 gagcagacct accagaccga cagggccatc atgagcgacg aacaaaagca gaaggccgag
     4681 gataatatcc tgcagagcaa gcgccgcttt cagttcgaac agcagtcgct gaaagaagac
     4741 gtccaggcta aacagcgcga gttgctgcag caggtgcaaa ccgagatcaa ggccgcgatt
     4801 caggcctacg gcgagtcgca gggctacgac ttcattttca ccgataccag tatcgccttc
     4861 gccagcgacg cggtcgatat caccgacgaa attctgcagg aactcaagaa gtaagccgca
     4921 gccggcgcca cgcagctggc cgagtggttg ccggccacgt cgcccggcga cattcgagcg
     4981 gttgactgcg agcgacgcgg cacgaagaaa ggcaatctca cctctacggc tgcgcctgtc
     5041 gagtgcagtc tct
//
LOCUS       DATCZV010000209         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_68156, whole genome shotgun sequence.
ACCESSION   DATCZV010000209 DATCZV010000000
VERSION     DATCZV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68156"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="VIW27_04725"
     CDS             <1..2143
                     /locus_tag="VIW27_04725"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEY5739004.1"
                     /translation="YWGDTHLHTNYSPDAYSLLTTTADPDTSYRFAKGLPVIADLSRA
                     RVQIRTPLDFLVVTDHAEFMGVISELAAGNEELMQTEFGQRWKPMLEDGRGGEVFFEM
                     IGAINTDPASIMPLNSEAIRKTVWESVVDIAETHNDPGVFTAFIGWEWSSVPDGRNLH
                     RIIFTPDAKDKAMQYLPYSLLDSAKPRDLYNWLAKTADEAGTDFVAIAHNMNLSAGSM
                     FPLFDESGNPIDLEYAELRERWEPVDEVSQYKGDSETHPKLSPNDPFADFETYEHLLD
                     PTVTEAPEPEAGDYARTALMRGLQMENALGKNPYKFGMVGATDSHTAFSSAEENNFLG
                     KYALDSIPENGDKETVPGAVGWDAAAQGLAGVWATENTREAITAAFKRKEVYASTGPR
                     ILLRVFGGYDFKAEHLQASDIATVGYRHGVPMGGDLAQPESGKPPSFLVQAAKDPKGA
                     NLDRVQIVKGWLNTDGTVEEKIYDLALSDGRTNGSEAVGNTVDLASASYTNEIGDPEL
                     AAVWTDPDFDPGLRAFYYVRVIEIPTPRHSVFDAVALGIDPSETGKPTTIQERAYSSP
                     IWYSPHSHRAKLAKILPQARESAPLVEDLLADGYLQMSGAEIGALIGKKLILRNRVSG
                     QEYTGMLLEGGRRVLSIVDGAASGPALQGLYHGGDPVLMGENGYAIVGDTVITSDGMR
                     TITSKLYRKDDKIVAARDVDSGRANFEVRAE"
     gene            2215..>3092
                     /locus_tag="VIW27_04730"
     CDS             2215..>3092
                     /locus_tag="VIW27_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5739005.1"
                     /translation="MLKPILRDPLLHFLVVAIGLFVLHDIFVHEDDSGNARQIVVDRD
                     ALLNFIQYRSKSFDQQVAERQLASLTDDELDKIITELVREEALAREARALGLDKDDYV
                     IKRRLVQKVDYVARGFADAAFKVTEEDIRAYFEENRADFYIQPRITFTHVFFSNERNG
                     ADHAEQLAAETLIRLREEGANFEEAGQYGERFLYGLNYVDRSRILVESHLGAEMVEAI
                     FGLDPTVSAWSGPFRSEHGAHAVLVTAVEPGRDPSLREVRVQVEQQASRALSERRARE
                     AAQLIVDAYDVEITYDP"
BASE COUNT          615 a          956 c          983 g          538 t
ORIGIN      
        1 gtactggggg gatacccacc tgcacaccaa ttactcgccg gacgcgtatt cgctgctgac
       61 caccacggct gatccggaca cctcttatcg tttcgccaag gggctgccgg tgatcgcgga
      121 tctcagccgg gcgcgggtgc agattcgcac gccgctcgat ttcctcgttg taaccgacca
      181 cgcagagttc atgggcgtga tttcggaact ggcggccggc aacgaggaac tgatgcaaac
      241 cgagttcggc cagcgctgga agccgatgct ggaagatggg cgtggaggcg aagtgttctt
      301 cgagatgatc ggggcgataa ataccgatcc cgcctcgatt atgcccctga actcggaggc
      361 gatacgcaaa acggtctggg agtcggtcgt cgatatcgcg gagacgcaca acgatccggg
      421 cgtgttcacc gctttcatcg gctgggaatg gagttcggtg cccgacggcc gcaacctgca
      481 ccgcattatc ttcacgccgg atgccaagga caaggcgatg cagtacctgc cttacagtct
      541 gctcgattcg gccaagccgc gggatctcta caactggctg gcgaagactg ccgacgaggc
      601 cggcaccgac ttcgtcgcca tcgcgcacaa catgaacctg tcggcgggct cgatgtttcc
      661 gctgttcgac gagagcggca atccgatcga tctcgagtat gccgagttgc gcgagcgctg
      721 ggagccggtc gacgaagtgt cgcagtacaa gggcgacagc gaaacccacc ccaagctgtc
      781 gccgaacgac ccgttcgccg atttcgaaac ctacgagcac ttgctggatc cgaccgtgac
      841 cgaggcgccc gaacccgaag cgggcgacta tgcgcgcacc gcgctgatgc gcggcctgca
      901 gatggaaaac gcactcggca agaaccctta caagttcggc atggtcggcg cgaccgactc
      961 gcataccgct ttttcctcgg cggaggaaaa taatttcctc ggcaagtacg cgctcgacag
     1021 tattcccgag aacggcgaca aggaaaccgt gccgggtgcg gtcggctggg acgccgcggc
     1081 gcagggcctg gctggcgtct gggctaccga gaacacgcgc gaggcgatca ccgccgcctt
     1141 caaacgcaag gaagtttacg cgagcacggg cccgcgcatc ttgctgcgcg tgttcggcgg
     1201 atacgacttc aaggctgagc acctgcaggc cagcgacata gcgaccgttg gctatcgcca
     1261 cggcgtgccg atgggtggcg acctggcgca gccggaaagc ggcaagccgc cgtccttcct
     1321 ggtgcaggcc gcgaaggatc cgaaaggcgc caacctggat cgcgtgcaga tcgtcaaggg
     1381 ctggctcaac accgacggta ccgtcgagga aaagatctac gatctcgcac tgtcggatgg
     1441 acgcaccaat ggcagtgaag cggtcggcaa cacggtcgat ctggccagcg ccagttacac
     1501 caacgaaatc ggcgatcccg agctcgccgc ggtgtggacc gaccccgatt tcgatccggg
     1561 cctgcgcgcg ttctactacg tgcgtgtcat cgagattccg acgccgcgtc actcggtgtt
     1621 cgacgcggtc gcgctcggca tcgatccgag cgaaaccggc aagccgacga ctattcagga
     1681 acgcgcctat tcgtcgccga tctggtattc accgcacagc caccgggcca aactggcgaa
     1741 gattttgccg caggcgcgcg aatccgcgcc gctggtcgaa gatctgctgg ccgacggtta
     1801 cctgcagatg agcggtgcgg aaatcggggc cctgatcggc aagaagctaa tcctgcgcaa
     1861 ccgggtcagc gggcaggagt acaccggtat gttgctggaa ggcggtcgtc gagtcctgag
     1921 tatcgtggac ggtgctgcaa gtggccccgc gttgcagggc ctgtatcacg gcggcgaccc
     1981 ggtactgatg ggcgaaaatg gctacgcgat cgtcggcgat accgtcatta cctccgacgg
     2041 catgcgcact atcacctcga aactctatcg caaggacgac aagatcgtcg ccgcgcgtga
     2101 cgtcgatagc ggccgggcca acttcgaggt gcgagccgag tagacctgcc gcctcgaaca
     2161 tccgtaatcc acaaacgcgg tccttcgggg ccgcgttcaa ttagaagacc tgacatgctg
     2221 aaacccattc ttcgagatcc gctgctgcat tttctggtgg tcgcgatcgg cctgttcgtg
     2281 ttgcacgaca tcttcgtcca cgaggacgac agcggcaacg cgcggcaaat cgtcgtcgac
     2341 cgcgacgcgc tgctgaattt catccagtac cgttccaagt ctttcgacca gcaagtcgcc
     2401 gagcgccagc tggcgagcct gacggacgat gaactcgaca agatcatcac tgagctggtg
     2461 cgcgaggaag ccctggcgcg ggaggcgagg gccctggggc tcgacaagga cgactacgtg
     2521 atcaagcgcc gcctggtgca aaaggtcgat tacgtggcgc gcggtttcgc cgatgccgcg
     2581 ttcaaggtca ccgaggagga tatacgcgcc tatttcgagg aaaaccgcgc ggacttctat
     2641 atccagccgc ggatcacctt tacccacgtc tttttcagta acgaacgcaa cggcgcggat
     2701 catgccgagc aactggccgc cgagaccctg atccggttgc gcgaggaggg cgcgaacttc
     2761 gaggaagcgg gccagtacgg cgagcggttc ctctacggtc tgaattatgt cgatcgctcg
     2821 cgcatcctgg tcgaaagcca tctcggtgcc gaaatggtcg aggcgatctt cggcctcgac
     2881 ccgacggttt ctgcctggag cgggccgttc cgttccgagc acggcgccca tgccgtgctc
     2941 gtcacggcgg tcgaacccgg ccgggatccg tcgctgcggg aagttcgtgt gcaggtggag
     3001 cagcaggcat cgcgggcgct gagcgaacgt cgcgcgcgcg aggcggcgca gctgatcgtc
     3061 gacgcctacg acgtcgaaat cacctacgac cc
//
LOCUS       DATCZV010000210         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_651448, whole genome shotgun sequence.
ACCESSION   DATCZV010000210 DATCZV010000000
VERSION     DATCZV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651448"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VIW27_04735"
     CDS             <1..241
                     /locus_tag="VIW27_04735"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960979.1"
                     /note="essential enzyme that recycles AMP in active cells;
                     converts ATP and AMP to two molecules of ADP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY5739006.1"
                     /translation="GAIYNVKSKPPKVDGVCDVCGGTDIVQRDDETEAAIENRLEVYN
                     LQTAPLVDFYRNEGLLVSLPTSEFEPVLETIKSRL"
     gene            complement(251..625)
                     /locus_tag="VIW27_04740"
     CDS             complement(251..625)
                     /locus_tag="VIW27_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739007.1"
                     /translation="MIAKILVTLAVIAACIWMLSARAKPQLREIPNPAEQRRRKQLRN
                     AAIAFMTFMVLAAATMIYLELDARGAVVTVHVVNTQSGETRSYRARRDEIHSDGFTTL
                     DGVEVFTAGIERIEIEATDSRQ"
     gene            complement(756..1802)
                     /locus_tag="VIW27_04745"
     CDS             complement(756..1802)
                     /locus_tag="VIW27_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739008.1"
                     /translation="MTSQVDVENLDAGYDDLQVLSQVSFALQSGQIGCILGPSGCGKT
                     TVLRVIAGFEAVWSGRIVIDRVEVSSTAGCLAPEKRNVGMVFQDFALFPHLSVEANVR
                     FGLRGMPARQQQARVQEMLAIVGMPAFAQAYPHQLSGGQQQRIALARALAPKPSVLLL
                     DEPFSNMDAELREQLAREVRNILKQESSTAILVTHDQNEAFAMADRICVMHEGVIQQQ
                     GSGYELYHQPVNRFVADFVGQGVIIPATVESATAVRTDLGVIDGDEPLGVEPGSMVDV
                     LIRPDDVLHDDASGESAIVVEKAFRGADFLYYLRMDSGIEVMCLAPSHHNHRINERIG
                     VRLDVDHLVIFSRG"
     gene            complement(1799..>2288)
                     /locus_tag="VIW27_04750"
     CDS             complement(1799..>2288)
                     /locus_tag="VIW27_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5739009.1"
                     /translation="IIPFAWFDNSVDALMRSNFDISTGLLLSGSLFAVMFAYMVRFLA
                     VSIQAVESGLLKIRRSMDEAARSLSYAPSEILLRVHLPLMKGTVLTAMLIVFVDVMKE
                     LPATLILRPFDFNTLAVRAFELASDERLADSSTAALAIVGVGLLPVILLSRSIGVSRA
                     GS"
BASE COUNT          444 a          736 c          710 g          398 t
ORIGIN      
        1 cggcgcgatc tacaacgtca agagcaagcc gccaaaagtc gacggcgttt gcgacgtctg
       61 cggcggcacc gacatcgtgc agcgcgacga cgaaaccgag gcagcgatcg agaaccggct
      121 cgaggtctac aacctgcaga ccgcgccgct ggtcgacttc taccgtaacg agggcttgct
      181 ggtgagcctg ccgacctcgg aattcgaacc ggtgctggaa accatcaaga gccgactgta
      241 gccgccgggt tcactgtcga ctgtccgtcg cctcgatctc gatgcgctcg atgccggcgg
      301 tgaatacctc gacgccgtcg agcgtcgtga agccgtcgct gtggatctcg tcccttcgtg
      361 cgcggtagga cctggtttcc cccgattgcg tattgaccac gtgcacggta accaccgcgc
      421 cgcgggcgtc gagttcgagg tatatcatgg tcgccgcggc cagcaccatg aatgtcataa
      481 aagcgatggc cgcgttgcgc agctgcttcc tgcggcgttg ttcggccggg ttggggattt
      541 cacgcaattg cggcttggcg cgcgccgaca gcatccagat gcaggcggcg attaccgcta
      601 gggtaacgag tatcttggcg atcatctaat tcccgacgat tgaatgcaac aggcgcggca
      661 cgggccgccg gtagacggcc aggcgcctgc cgaaagaaag ccctatttta tggttatgcc
      721 ggcggcgcgc aaccgcggcg tcacgcggct cgccatcagc cgcgcgaaaa tataaccagg
      781 tgatcgacat cgaggcgaac gccgatgcgc tcgttgatgc ggtgattatg gtggctcggc
      841 gccaggcaca tgacctcgat accgctgtcc atgcgcaggt aatagagaaa atcggcgccg
      901 cgaaacgcct tttcgacgac gatcgccgac tcgccggagg catcatcgtg caagacgtcg
      961 tccggacgga tcaggacgtc gaccatgctg cccggctcga cgccgagcgg ctcgtcgccg
     1021 tcgatcacgc cgaggtcggt tctgaccgcg gtcgcgctct cgacggtcgc cgggatgatc
     1081 acgccctggc cgacgaaatc ggcgacgaag cggttgaccg gctgatggta aagctcgtaa
     1141 ccgctgccct gctgctgaat gacgccctcg tgcatgacgc aaatgcggtc ggccatcgca
     1201 aaggcttcgt tctgatcgtg ggtgaccaga atcgcggtgc tgctttcctg tttgaggatg
     1261 ttcctgacct cgcgcgccag ctgttcgcgc aattccgcgt ccatgttcga aaacggttcg
     1321 tcgagcagca gcaccgacgg tttcggtgcc agcgcccgag ccagcgcgat gcgctgctgc
     1381 tgcccgccgg acaactggtg cgggtaggcc tgcgcaaagg ccggcatgcc gacgatcgcc
     1441 agcatttcct gcacgcgcgc ctgctgttgc ctggccggca ttccgcgcag gccgaatcgc
     1501 acgttggctt cgacgctgag gtgcgggaac agcgcgaaat cctggaacac catacccacg
     1561 ttgcgctttt ccggtgccag gcagcctgcc gtggaactga cttccacgcg gtcgatcacg
     1621 atgcgtcccg accacaccgc ttcgaagccg gcgatcacgc gcaacacggt cgtcttgcca
     1681 caaccgctgg ggccgagtat gcagccgatc tgcccggatt gcagcgcaaa gctgacctgg
     1741 ctcaacacct gcagatcgtc gtatccggcg tccaggtttt cgacatcgac ctgcgaagtc
     1801 acgagccggc cctcgatacg ccgatactgc ggctgagcag gattaccggc aacaggccga
     1861 cgccgacgat cgccagcgcc gcggtcgacg aatccgcgag gcgctcgtcc gaggccagct
     1921 cgaaggccct gaccgccagc gtgttgaagt cgaacggccg caggatcagg gtcgccggca
     1981 gctccttcat gacatcgacg aaaacgatca gcatcgcggt cagcaccgtg cctttcatca
     2041 gcggcaggtg tacccgcagc agaatttccg acggcgcata ggacagcgaa cgcgccgcct
     2101 cgtccatcga gcgcctgatc ttcagcaggc cggactccac cgcctggatc gataccgcca
     2161 gaaagcgcac catgtacgcg aacatcaccg cgaacaggct gccggagagc agcaggccgg
     2221 tggaaatatc gaaattcgag cgcatcagtg catcgacgct attgtcgaac caggcgaacg
     2281 gaatgatc
//
LOCUS       DATCZV010000211         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_121721, whole genome shotgun sequence.
ACCESSION   DATCZV010000211 DATCZV010000000
VERSION     DATCZV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121721"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="VIW27_04755"
     CDS             <1..1725
                     /locus_tag="VIW27_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739010.1"
                     /translation="VVISDGAAQSPASLQSELLALRAAGIPVYSLGIGAEHYARDIEL
                     TAVQMPGSVLRGSRLLAAVTVSQQGYDGTTLELRVEDDYRILHKQPIRLDTASQRFEV
                     PLAAADAGARQLKFSVAAQPGEKLVDNNSRIAMLAVERERQRILYYEGEPRFELKFLR
                     RAVADDRQLAVAGLIRTADAKYYRVGIESSDELRDGFPTTRDELFAYDAIILGSVEVS
                     LLDREQQQMIVDFVSQRGGGLLFLGGRHAFAEGGYHDSPLRELFPVFIDAPPQNDFSR
                     RIKIRPTPAALVHPALRLDEDERKSADRWLTLPALTTVNPLRRIKPGATLLLAGEVEN
                     ENANEGYVAMAFQRYGRGKVVAFAVQNSWLWQMHHEIDLDDQSHEILWRQLLRWLVES
                     VSGRIELTLSDETLHIGGNLRLRGEVLQADFDVHQAAEVRAVLVSPTGFERIVPLARN
                     PALRGVYEAEIIAAEAGDYRVHLELDEAGETLRGAEARFVVSAAGDEYFGSQMNARLL
                     GDIAAQTGGEFFAAARADDLPRALADAQRGARLLTRHQLWDMPLLFMLLVLLLCAEWA
                     YRRWRGLV"
     gene            1722..>2204
                     /locus_tag="VIW27_04760"
     CDS             1722..>2204
                     /locus_tag="VIW27_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739011.1"
                     /translation="MRRALSICLLLLLSLPATAGRHQLLIVSGLGGTTAYGEQFTGLA
                     TRLAQAARDAGVASEQIVTLSASANAAGARADRDTVLAAISRAAETLGEDDRFFLVMF
                     GHGNPRGDGAVFNLPGPDLTATELAAALEAFGERLVIVVNTASSSGPFVAPLAAPNRV
                     V"
BASE COUNT          371 a          758 c          719 g          356 t
ORIGIN      
        1 gtagtgatca gcgacggcgc cgcgcaatct ccggcatcgc tgcagtccga gctgctggcg
       61 ctgcgcgccg ccggcatacc ggtatacagt ctcggcatcg gcgccgagca ctatgcgcgc
      121 gacatcgaac tgactgcggt acagatgccg gggagcgtat tgcgcggcag ccgcctgctc
      181 gccgcggtta ccgtcagcca gcagggctac gatggcacca cgctggaact gcgggtcgag
      241 gacgactacc gcatcctgca caagcaaccc atcagactcg ataccgcctc acagcgcttc
      301 gaagtgccac tggcagccgc cgatgccggg gcgcggcaac tcaaattcag tgtcgccgcg
      361 cagcccgggg aaaaactggt cgacaataac agccgcatcg cgatgctggc cgtcgagcgc
      421 gagcgccaga gaatcctcta ctacgagggt gaaccgcgct tcgaactgaa attcctgcgc
      481 cgcgcggttg ccgacgaccg tcagctggcg gttgccgggc tgattcgcac cgccgacgcc
      541 aagtattacc gcgtcggcat cgagtcgagc gacgaactgc gtgacggctt tccgaccact
      601 cgcgacgagc tgttcgctta cgacgcaata atcctaggca gcgtcgaggt gtcgctgctg
      661 gatcgcgaac agcagcaaat gatcgtcgat ttcgtcagcc agcgcggcgg cggactgctg
      721 ttcctcggcg ggcgccacgc gttcgccgag ggtggctatc acgactcgcc actgcgcgag
      781 ctgttcccgg tattcatcga tgcgccgccg caaaacgatt tttcgcgccg catcaagatc
      841 cggccgaccc cggcggcact ggttcacccg gcgctgcggc tcgacgagga cgagcgcaaa
      901 tcggccgacc gctggttgac cctgccggcg ctgaccaccg tcaatccgct gcgccgcatc
      961 aagccgggcg ccacgctgct gctcgccggt gaggtcgaga acgaaaacgc caacgaaggc
     1021 tacgtggcaa tggcctttca acgctacggg cgcggcaagg tggtagcctt cgcggtgcaa
     1081 aattcctggc tttggcaaat gcaccacgaa atcgatctcg acgaccagag ccacgaaatt
     1141 ctgtggcgcc agctgttgcg ctggctggtg gaatcggtat ccgggcgtat cgaattgacg
     1201 ctgtccgacg aaaccctgca catcggcggc aacctgcggc tgcgcggcga agtgctgcag
     1261 gccgatttcg acgtccacca ggccgccgag gtgcgcgcgg tgctggtttc gccgaccggc
     1321 ttcgaacgaa tcgtgccgct ggcgcgcaac cccgccctgc gcggcgttta cgaagcggaa
     1381 atcatcgccg ccgaagccgg cgactatcga gtgcatctcg aactcgatga agccggcgag
     1441 acactgcgcg gcgccgaggc gcgattcgtc gtcagcgccg ccggcgacga atatttcggt
     1501 tcgcagatga acgcgcgcct gctgggcgac atcgccgcgc aaaccggcgg cgaattcttt
     1561 gccgcggcgc gcgccgacga tctgccgcga gcgctggccg acgcccagcg tggcgctcgg
     1621 ctgctcaccc gccaccaact ctgggacatg ccgctactgt tcatgctgct ggtcctgctg
     1681 ctgtgcgccg agtgggccta ccgccgctgg cgagggctgg tatgagacgc gcgctgtcga
     1741 tctgcctgtt gctgctgctg agcctgcccg ccaccgccgg ccggcaccag ctgctgatcg
     1801 tcagcggcct gggcggcacc accgcctatg gcgagcagtt caccgggctg gcgacgcgcc
     1861 tggcacaggc cgcgcgcgac gccggcgtcg ccagcgagca aatcgtcacg ctgtcggcga
     1921 gcgccaacgc cgcgggcgct cgcgccgatc gcgacacggt gctggcggcc atctcgcgag
     1981 ccgccgagac cctcggtgaa gacgatcggt ttttcctggt aatgttcggc catggcaatc
     2041 cacgcggcga cggcgcggtg ttcaacctgc ccggacccga tctcaccgcc accgagctgg
     2101 ccgcggccct cgaagccttc ggcgagcggc tggtgatcgt ggtcaacacg gcctcgtcga
     2161 gcggcccgtt cgtcgccccg cttgcggcgc ccaaccgggt ggtg
//
LOCUS       DATCZV010000212         4755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_68802, whole genome shotgun sequence.
ACCESSION   DATCZV010000212 DATCZV010000000
VERSION     DATCZV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68802"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VIW27_04765"
     CDS             complement(<1..561)
                     /locus_tag="VIW27_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY5739012.1"
                     /translation="MRLIVHGQQAYGKSVLEAILEKDVDEVVAVYCAPDKEGRPLDPL
                     KEFALEKDLPVFQPDSYKNPDVWEQLASHDADLCVMAYVLLFVPEEALNVPKYGSIQY
                     HPSLLPWHKGPSSINWPIIMGKEKTGLTIFWPDNGLDTGPILLQKEVDIGPDDTLGSV
                     YFDKLYPLGVEAMMESIELVRDGNAPR"
     gene            complement(612..1862)
                     /gene="frc"
                     /locus_tag="VIW27_04770"
     CDS             complement(612..1862)
                     /gene="frc"
                     /locus_tag="VIW27_04770"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY5739013.1"
                     /translation="MSKPLDHIKILDFTHVQSGPTCTQLLAWMGADVIKVERPGVGDA
                     TRKQLVDVEGADSLYFTMLNHNKRSLTLNTKHESGKEVLERLIKTCDVLVENFAPGAL
                     DRMGFGWERIQELNPAMIMASVKGFGPGPYEDCKVYENVAQCAGGAASTTGFLGGLPL
                     VTGAQIGDSGTGLHLAFGICAALIHREKTGKGQRVLAPMQDGVLNLCRVKLRDQQRLK
                     AGPLKEYSQYGEGIPFGEAAPRAGNDSGGGQPGRILKCKGWETDPDAYTYFITQAAVW
                     EKICDVIGKPEWKEDPDFATPPARLPRLNEVFGAIEEWTMTKTKYEVMEICNPLDIPV
                     GPILSMKELAEEQSLRETGTIVECDHPERGPYLSVGCPVKLSDNDVTVTRSPLLGEHT
                     DEVLTEVLGYSAEEIAAFKEAGAV"
     gene            complement(1903..3651)
                     /locus_tag="VIW27_04775"
     CDS             complement(1903..3651)
                     /locus_tag="VIW27_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5739014.1"
                     /translation="MATKAQEVSSTAHDTLAQKTKDGSVISGGHLVAKALKAEGVDTI
                     FTLCGGHIIDIYDGCLDEGIRIVDVRHEQTAAHAADGYARQTGKLGCVVTTAGPGCTN
                     AVTGIATAYRSESPILHIGGQSSLTQHKMGSLQDLPHVDMMTPITKFASGVFSTERVA
                     DMISMAARECFNGAYGPSYLEIPRDILDREIPIENAVIPKPGHYRASVKSIGDPADIE
                     KLADILVKSKSPAVLFGQQVWVSGGAQQAIDFVRALDIPAYMNGASRGMLPRDDPHHF
                     DRTRSDAFKNADVILIVGTPFDFRMGYGKRISQDATLVQIDQSYATVGKNRDISLGLA
                     GDPGAILAAVAQAASGRIDDGARKQRQKWMAQLRDIEATKLEKLMPMFTTDQNPIHPY
                     RLAYEINEFLGEDTIYIGDGGDIVTISAQAVRPRNPGQWMDPGALGSLGVGTGFSMAA
                     KLAHPDKEVICLYGDGAFGMTSFDMETAQRFGAPYLAVVGNNSAMNQIRYGQLAKYGQ
                     DRGDVGNKLGDVPFSKFGEMIGGYGEEVTEAKEIQPALQRAREAIARTGRCAVVNVWV
                     DPNEYAPGTKAQTMYK"
     gene            complement(3701..4441)
                     /locus_tag="VIW27_04780"
     CDS             complement(3701..4441)
                     /locus_tag="VIW27_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5739015.1"
                     /translation="MRKAGTDNKKAAKPEPAGGVRVVPIGATSVLKDQVYAILREAVS
                     NMDIYSTPEPPRLDERKLAEELGVSRTPVREALSRLEQEGFVRNIPRRGTFVVRKTKR
                     EVLEVVYAWAALESMAARLATERASDEEIGRLREIFATFDDEEEARAHINEYSDTNIE
                     FHQTLIGLSQCELIKTMSSSLFLHMRAIRVQALKDTEHHRSDQSVIDHMRIIEALEAR
                     DADRVERLVREHALSLSKHIEEYAQYLD"
     gene            complement(4470..>4755)
                     /locus_tag="VIW27_04785"
     CDS             complement(4470..>4755)
                     /locus_tag="VIW27_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY5739016.1"
                     /translation="QTMHDVWLMFLFGVAGYLLRKLDYPVAPAVLAIVLGPLAERSLR
                     QSLLASQGDPSIFLTRPISLACILIAVALVMVPVVQKIKRRRKKAAEQQA"
BASE COUNT          803 a         1499 c         1453 g         1000 t
ORIGIN      
        1 cctcggcgcg ttaccgtcac gcaccagttc gatcgactcc atcatcgcct cgaccccgag
       61 cgggtacagc ttgtcgaagt aaaccgaacc gagggtgtcg tcggggccga tatcgacttc
      121 cttctgcagc aggatcggtc cggtatcgag accgttatcc ggccagaata tggtcaatcc
      181 ggttttttcc ttgcccatga tgatcggcca gttgatcgag ctcggaccct tgtgccaggg
      241 cagcagcgac gggtggtact gaatcgagcc gtacttcggc acgttgagcg cctcttcggg
      301 cacgaacagc aatacgtacg ccatcacgca caggtcggca tcgtgactgg cgagctgctc
      361 ccagacgtcc gggttcttgt aggaatcggg ttgaaatacc ggcaaatctt tctccagcgc
      421 gaattccttg agcgggtcca gcggcctgcc ttccttgtcg ggcgcgcagt aaaccgccac
      481 cacttcgtcg acgtctttct cgagaatcgc ctcgagaaca gacttgccat aggcctgctg
      541 accatgaacg attagtctca tcattacctc cagttaccgc ggatgacacg tgccacccgg
      601 ggtttaatta tttaaacggc gccggcttct ttaaaggccg cgatctcttc cgcgctgtat
      661 ccgagcacct cggtaaggac ttcatcggta tgctcaccga gcagcggcga gcgcgtaacc
      721 gtaacgtcgt tgtccgacag cttgaccggg caacccaccg aaaggtaagg cccgcgctcg
      781 gggtgatcgc attcgacgat agtgccggtt tcacgcagcg actgctcctc ggccagttcc
      841 ttcatcgaca ggatcggccc caccgggatg tcgagcggat tgcagatttc catgacttcg
      901 tacttggttt tggtcatggt ccattcctcg atcgcgccga acacttcgtt gagccgcggc
      961 aggcgcgccg gcggggtcgc gaagtcgggg tcttccttcc actccggctt gccgatgaca
     1021 tcgcaaatct tttcccagac cgccgcctgg gtaatgaaat aggtataagc gtcgggatcg
     1081 gtttcccagc ccttgcactt gaggatgcgg cccggctgcc cgccgcccga atcgttgccg
     1141 gcgcgtggcg cggcctcgcc gaacggaatg ccttcgccat actgcgagta ctccttcagc
     1201 ggccccgcct tgagccgctg ctggtcgcgc agcttgacgc ggcacaggtt gagcaccccg
     1261 tcctgcatcg gcgccagcac gcgctgaccc ttgccggtct tttcacggtg aatcagcgcc
     1321 gcgcagatac cgaaagcgag gtgcaggccg gtgcccgagt cgccgatctg cgcgccggta
     1381 accagcggca ggccacccag aaaaccggtc gtcgacgcgg cgccgccggc gcactgggcg
     1441 acgttttcgt aaaccttgca atcctcgtac gggccgggac cgaagccctt gaccgaggcc
     1501 atgatcatcg ccgggttcaa ttcctggatg cgctcccagc cgaaacccat gcggtcgagc
     1561 gcaccgggcg cgaagttttc aaccagcacg tcgcaggtct tgatcaggcg ctcgaggact
     1621 tccttgccac tctcgtgctt ggtattcagg gtcagcgagc gcttgttgtg gttgagcatc
     1681 gtgaaataca ggctgtcggc gccttcgacg tcgacgagtt gcttgcgggt cgcgtcgccg
     1741 acgccgggac gttccacctt gatcacatcg gcgcccatcc aggcgagcag ctgggtacag
     1801 gtcggaccgg actggacatg cgtgaagtcc aggattttta tatggtcgag tggctttgac
     1861 attttctaca tctacctctg ttgatcgcgc tgcgatatcg gtttacttgt acatggtttg
     1921 cgccttggtg ccgggcgcgt attcgttcgg atccacccac acgttgacca ccgcgcaacg
     1981 gccggtcctg gcgatcgctt cgcgggcgcg ctgcagcgcg ggctggattt ccttcgcctc
     2041 ggtgacttcc tcgccgtagc cgccgatcat ttcgccgaac ttcgagaacg gcacgtcgcc
     2101 gagcttgttg ccgacgtcgc cgcggtcctg accgtacttg gcgagctggc cgtagcgaat
     2161 ctggttcatc gccgagttgt tgccgacgac cgcgaggtat ggcgcgccga agcgctgcgc
     2221 ggtttccatg tcgaacgagg tcatgccgaa ggcaccgtcg ccgtacaggc agataacctc
     2281 tttatcggga tgcgcgagct tggccgccat cgagaagccg gtaccgacac cgagcgaacc
     2341 gagcgcaccg ggatccatcc attgccccgg attgcgcggg cgtaccgcct gcgccgaaat
     2401 ggtcacgatg tcgccgccgt cgccgatgta gatcgtgtcc tcgccgagga actcgttgat
     2461 ctcgtaggcg aggcggtagg gatggatcgg gttctggtcg gtggtgaaca tcggcatcag
     2521 cttttccagc ttggtcgcct cgatgtcgcg cagctgcgcc atccacttct ggcgttgctt
     2581 gcgcgcaccg tcgtcgatgc ggcctgatgc agcctgcgcg acggcggcca gtatcgctcc
     2641 gggatcgccg gcgaggccga ggctgatgtc acgattcttg ccgaccgtgg cgtagctctg
     2701 atcgatctgt accagcgtgg cgtcttgcga gatgcgcttg ccgtagccca tgcggaaatc
     2761 gaacggcgta ccgacgatca ggatcacgtc ggcattcttg aacgcgtccg agcgtgtgcg
     2821 gtcgaaatgg tgcgggtcgt cccgcggcag catgccgcgg ctggcaccgt tcatgtaggc
     2881 cggaatatcg agcgcgcgca cgaaatcgat cgcctgctgc gcgccgccgg aaacccagac
     2941 ctgctggccg aacagcaccg ccgggctctt cgacttgacc aggatatcgg cgagcttctc
     3001 gatgtcggcc gggtcaccga tcgacttcac cgaagcgcgg taatgtccgg gcttcgggat
     3061 caccgcgttt tcgatcggaa tctcgcgatc gagtatgtcg cgcggaattt cgaggtagga
     3121 aggcccatag gcgccgttga agcattcgcg cgcggccatc gaaatcatgt cggcgacacg
     3181 ctcggtggag aatacgcccg atgcgaactt ggtgatcggc gtcatcatat cgacgtgcgg
     3241 cagatcctgc agcgagccca tcttgtgctg ggtcagcgaa ctctggccgc cgatgtgcag
     3301 gatcgggctt tccgaacggt aagcggtggc gatgccggtc accgcgttgg tgcagccggg
     3361 ccccgcggtg gtgaccacgc agccgagctt gccggtctgg cgcgcgtagc cgtcggcggc
     3421 gtgcgcggcg gtctgctcgt gacgcacgtc gacgatgcga atgccctcgt cgaggcagcc
     3481 gtcgtagata tcgatgatgt gcccgccaca gagcgtgaaa atggtgtcga cgccttcggc
     3541 tttcagtgct tttgccacca ggtgtccgcc cgaaatgacc gatccatcct tggttttctg
     3601 cgccagagta tcgtgagcgg tgctgctgac ttcttgagct ttggttgcca ttgcttgttt
     3661 cctcgttgct acgggccggg ctgttccggc cgggttttgt ctagtcgaga tactgcgcgt
     3721 attcctcgat gtgtttactt aaggagagcg catgttcgcg caccaacctt tcgacccggt
     3781 cggcgtcgcg cgcctccagg gcctcgataa ttcgcatgtg gtcgatcacc gactgatccg
     3841 atcgatgatg ctcggtatcc ttgagcgcct gcacgcgaat cgcgcgcatg tgtaaaaaca
     3901 ggctgctcga catggtcttg atcaactcgc actggctcag accgatcagg gtctggtgaa
     3961 actcgatgtt ggtatccgag tattcgttga tgtgcgcgcg cgcttcctcc tcgtcgtcaa
     4021 aagtcgcgaa gatctcgcgc agcctgccga tttcctcgtc gctggcgcgc tcggtcgcga
     4081 gccgcgccgc catgctttcc agcgccgccc aggcatagac cacttcgagc acctcgcgct
     4141 tggtcttgcg caccacgaag gtaccgcggc gcggaatatt cctgacgaaa ccttcctgct
     4201 cgagcctcga cagcgcctcg cgtaccgggg tacgactgac gccgagttcc tcggccagct
     4261 tgcgctcgtc gagccgtggc ggttcgggtg tcgagtagat atccatgttg ctgacggctt
     4321 cccgcaggat cgcatacacc tgatccttga ggacgctggt cgcgccgatc ggcactaccc
     4381 gcacgccgcc cgccggttcc ggctttgccg ctttcttgtt gtcggttccg gctttgcgca
     4441 catcggtctc cattcgccag gcggccgcct caggcctgct gttccgcggc cttcttcctg
     4501 cgccgcttga tcttctgcac taccggcacc atcaccagcg cgaccgcgat cagaatgcaa
     4561 gccagagaaa tcgggcgggt aaggaaaatc gacgggtcgc cctgcgacgc caacaacgat
     4621 tgcctcagtg agcgctcggc gagcggcccg agcacgatcg ccagcaccgc cggtgcaacc
     4681 gggtagtcga gctttctgag caaatagccg gccacgccaa acaggaacat cagccagacg
     4741 tcgtgcatgg tttgc
//
LOCUS       DATCZV010000213         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_492424, whole genome shotgun sequence.
ACCESSION   DATCZV010000213 DATCZV010000000
VERSION     DATCZV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492424"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..985)
                     /locus_tag="VIW27_04790"
     CDS             complement(338..985)
                     /locus_tag="VIW27_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5739017.1"
                     /translation="MNLAHETPQASIWVIDDDQSMRWVLSKTLTNSGYRVTAFESGSL
                     ALASFKRAAADERPNLMITDVRMPGINGFELLKQVKNISPQTPIIVMTAYTDLDTTKQ
                     AFHEGAFEYLPKPFDIDEAIELVARACAQANEQGSRASQPLPSEAGDWQSPLRMWARQ
                     QLSQGETDILKNAARTFEKTLLECALEATRGRKQDAARLLGWGRNTLTRKLKELG"
     gene            complement(982..2040)
                     /gene="glnL"
                     /locus_tag="VIW27_04795"
     CDS             complement(982..2040)
                     /gene="glnL"
                     /locus_tag="VIW27_04795"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY5739018.1"
                     /translation="MTPTPDSGSRILDTLKAALLCLDNERRITYVNATAELLFDNSAA
                     SLVGRPFDSLLSRSEPSTISQNLAREPISMTEHEAVITLASGKSFIADYSIYSDSERD
                     PDSDILLEIRLLDRQAQIAREEFNQLQQLARQQLARGLAHEINNPLGGIRGAAQLLQR
                     ELENPDLLEYTEVIISEVDRLRALIANMLGPEIRPQRKPINIYEVIEHIRKIIQAAEP
                     ERIRIHRDYDPSIPQMMADRDLLIQAFLNIARNAVQAIDGEGDITFRTRVDRNYTIGQ
                     TNHPLVVKIDIIDSGCGISSELGDSIFLPMITDKPDGSGLGLPIAQEIIFRHGGSLQM
                     QSSSEGTTFSTILPMGQS"
     gene            complement(2152..>2800)
                     /gene="glnA"
                     /locus_tag="VIW27_04800"
     CDS             complement(2152..>2800)
                     /gene="glnA"
                     /locus_tag="VIW27_04800"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925545.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY5739019.1"
                     /translation="TFMPKPIFGDNGSGMHTHQSIWKDGKPTFAGSGYADLSETALFY
                     IGGIIKHARAINAFANAATNSYKRLVPGFEAPVLLAYSARNRSASIRIPYVSSPKARR
                     IEVRFPDSTANPYLAFSAMMMAGLDGIQNQIHPGDAMDKDLYDLPPEEERDIPKVAFS
                     LDEALEALDQDREFLKAGGVFTDDVIDGYIGLKMDDVERMRLATNPVEFDLYYSV"
BASE COUNT          474 a          804 c          923 g          599 t
ORIGIN      
        1 ggcgtacgat actctacagc acgcacttgc tcgccgatgc cgaggatctg tgcgatcagt
       61 tcggcatttt gcacaacggc gaaatgaagt accggggcac accggcggat tgcatcgcgc
      121 gctacgccac cgaaacgctc gagcaggcct acatgaagtg catatccgca tgaccgggcg
      181 agcgcgagcc acgcggcggg cggagaggcc cgatcctggt ctggcgcgaa tcaacggacg
      241 ataaagcgaa tacacctgtg ccggttttgc gttagccgcg ccatcgcccg cggcggataa
      301 atgccgcact gtgttggcgc gacccgcagc ggtcggctca gcccagttcc ttcagcttgc
      361 gggtcagcgt attgcggccc cagccgagca ggcgcgcggc gtcctgcttg cggccccggg
      421 tggcttccag cgcgcactcg agcagggttt tctcgaaggt cctggcggcg tttttcaata
      481 tatcggtctc accctgcgac agttgctgcc gtgcccacat gcgcagcggt gattgccagt
      541 cgccggcttc cgacggcagc ggttgcgagg ctcggctgcc ctgctcgttc gcctgcgcgc
      601 aggcgcgcgc gaccagctcg atcgcctcgt cgatatcgaa gggtttcggc aggtactcga
      661 acgcgccttc gtgaaacgcc tgcttggtgg tgtcgagatc ggtgtaggcc gtcatcacga
      721 tgatcggcgt ctgcggcgag atgttcttga cctgcttcaa caattcgaag ccgttgatgc
      781 cgggcatgcg cacgtcggta atcatcaggt tcggtcgttc gtcggcggcc gcgcgcttga
      841 agctcgccag agcgagcgag ccggactcga aggcggtcac ccggtagccg ctgttggtca
      901 gggttttact cagtacccag cgcatcgatt ggtcgtcgtc gatgacccag atgctggctt
      961 gcggagtttc atgtgctaga ttcatgattg tcccatcgga agaatagtgc tgaaagtggt
     1021 cccctcgctg gagctttgca tctgcaggct accgccgtgg cgaaagatga tttcctgggc
     1081 gattggcagg cccagtccgc tgccgtccgg cttgtcggtg atcatcggta aaaaaatcga
     1141 gtcaccgagt tcgctggata tgccgcagcc gctgtcgatg atgtcgatct tgaccaccag
     1201 cggatggttg gtctggccga tggtgtaatt gcggtcgacc cgggttcgaa aggtaatatc
     1261 gccttcaccg tcgatcgcct gcacggcatt gcgcgcgata ttcagaaacg cctggatcag
     1321 cagatcgcgg tcggccatca tttgcggaat gctgggatcg tagtcgcggt gaatgcgaat
     1381 gcgctcgggc tcggccgcct ggatgatctt gcgaatatgc tcgatgacct cgtagatgtt
     1441 gatcggcttg cgctgcggcc ggatttcggg tcccagcatg ttggcaatca gtgcccgcaa
     1501 gcggtcgact tcgctgatga tgacctcggt atactcgagc aagtccggat tttcgagttc
     1561 gcgttgcagc aattgcgccg cgccgcgaat gccgcccaac ggattgttga tttcgtgtgc
     1621 cagtccgcgc gccagctgtt gcctggcgag ttgctgcagc tggttgaatt cctcgcgcgc
     1681 gatctgcgcc tggcggtcga gcaggcggat ttcgagcagg atgtcgctgt caggatcgcg
     1741 ctcactgtcc gaatagatgc tgtagtcggc gatgaaagac ttgccgctgg cgagcgtgat
     1801 caccgcttca tgctcggtca tcgatatcgg ttcccgcgcc aggttctggc tgatcgtcga
     1861 cggttcgctg cggctcaaca ggctgtcgaa tggccgcccg acgaggctcg cggcgctgtt
     1921 gtcgaacagc agttcggcgg tcgcgttgac gtaggtgatg cggcgctcgt tatccaggca
     1981 aagcagggcc gccttcagcg tatccaggat gcgactgccg gaatcgggtg tcggtgtcat
     2041 gctgcgggcc atcgatagct gcatggaatt gcgcgaggca tttctgcggg caatgccgag
     2101 acaaaaaagg ccccgtcggg ggcctttcct gtcctagctt ccagcccggg attaaacgct
     2161 gtagtagagg tcgaactcga ccgggttggt tgccagccgc atgcgctcga cgtcgtccat
     2221 cttcaggccg atgtagccgt cgatgacgtc atcggtgaac acgccaccgg ccttgaggaa
     2281 ttcgcggtcc tggtccaggg cctcgagcgc ctcgtcgagc gagaaggcga ccttcggaat
     2341 atcccgctct tcctctggcg gcaggtcgta gaggtccttg tccatcgcgt cgccgggatg
     2401 gatctggttc tggatgccgt cgaggccggc catcatcatc gcgctgaagg cgaggtaggg
     2461 gttggcggtg gaatccggga agcgtacttc gatgcggcgt gccttggggc tgctgacgta
     2521 cggaatgcga atcgatgccg aacggttacg tgccgagtag gccagcagta ccggcgcttc
     2581 gaagcccggc accaggcgct tgtagctgtt ggtcgcggcg ttggcgaagg cgttgatcgc
     2641 gcgcgcatgc ttgatgatgc cgccaatgta gaacagcgcg gtttccgaaa ggtccgcgta
     2701 accggagccg gcgaaggtcg gcttaccgtc cttccagatg gactgatgtg tgtgcatgcc
     2761 cgacccgttg tcgccgaaga tcggcttggg catgaaggtg
//
LOCUS       DATCZV010000214         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_598754, whole genome shotgun sequence.
ACCESSION   DATCZV010000214 DATCZV010000000
VERSION     DATCZV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598754"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1200)
                     /locus_tag="VIW27_04805"
     CDS             complement(142..1200)
                     /locus_tag="VIW27_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5739020.1"
                     /translation="MSISINRHAGPASIRAWSMALLLAAAACLQAGGVRAGGQVWGAD
                     YFPNYELTAHTGEKLRFFDDVIAGKVVAINFIFTNCTDICPMETARLADVYRLLGDRV
                     GEDVHFYSITIDPQNDTVAVLNEYADRFGIDGRNWKLLTGDKRQIDHIREKFGLYGTA
                     AEEAELSNHNINLVLGNQSTGHWVRRSPFENPYILANQLGSWLHGGKVTSAHTRRNYA
                     EAPSLRQISAGEIMFRDRCSSCHVISGGLAPTRKAKALGPDLYAVSQRRDPQWLRRWL
                     AEPDVMLAEGDPIASALYAQYKVIMPNFRLDASEIEALIDYMASETERLRSSKLAAGD
                     PVSQLPKPNAGVAEHAGH"
     gene            complement(1266..>2878)
                     /locus_tag="VIW27_04810"
     CDS             complement(1266..>2878)
                     /locus_tag="VIW27_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY5739021.1"
                     /translation="WPMILAGHDHINTDASDPRAATPDGAGGTIRIPGDYRETMSTHW
                     FHDHMLDFTAQNVYKGSAAMMNYYSALDRGNEAVDDGVNLRLPSGSALPWGNRDYDVN
                     LLLAAKAWDDEGQLWFNIFNKDGFLGDQMTVNWLYKPYFDVRARRYRFRILNGSVSRY
                     LKLALVQEVVGHRSGEIPGRRSQGVSWNRIPFHMVANDGNIMEHTVPFDGSVDLDGDG
                     NADEHRGTLPVQAIAERFDIVVDFAAHGLQPGDKLYFVNLLEHKNGKVVEGSVDLGAV
                     LRETYKAELDYDDGVAARWENGDPVVGKFLEFRIKAYGGTDLSMNPADYVPGGQKMIS
                     LVIDRDDPVQMAEVADARRRTFVFGRSSGTDSAPWTIKTDGGSAYTADTRRLTAAPQL
                     ANGPTPGGYAGDGTAEIWRFETGGGWSHPIHVHFEEGVILTEDGETPPEWHRWARKDV
                     FRLGPEEDAAREVEVFYNFREFAGSYVEHCHNTQHEDHAMLLRWDLEQPGQVQIMPAP
                     IPTWDGVEYVDSIALPTFRSGEGFGLQP"
BASE COUNT          533 a          911 c          878 g          556 t
ORIGIN      
        1 tcgaaaaggc cgccgacgcg gtggtggagg cgccgaacag ggatccggca gccagcatta
       61 tgggaacaag tttgattttc attgagttat ccctcggtaa attcgaaata aaaaaatttg
      121 caacagtaaa tcctatcgat atcaatggcc cgcatgctcc gcaacgccgg cgttcggctt
      181 cggcagctgc gacaccggat caccggcggc cagcttactg ctgcgcagac gctcggtttc
      241 actggccatg taatcgatca gcgcctcgat ctcgctcgca tccaggcgaa aattgggcat
      301 gatcaccttg tactgggcat agagcgcgga cgcgatcgga tcgccctcgg cgagcatcac
      361 gtcaggctcg gcgagccagc gccgcagcca ctgcggatca cgccgctggc tcaccgcgta
      421 caggtcgggg ccgagcgcct ttgctttacg tgtcggcgcg agcccgccgc tgatcacgtg
      481 gcacgacgaa caacggtcgc gaaacatgat ctcgccggcc gaaatctgcc gtaacgacgg
      541 ggcttcggca taattccggc gcgtgtgcgc actggtcacc ttgccaccgt gcagccagga
      601 gcccagctga ttggcgagga tataggggtt ttcaaacggc gagcgcctga cccagtgccc
      661 ggtcgactga ttgccgagca ccaggttgat attgtggttc gacagctcgg cttcctcggc
      721 ggccgtgccg tagaggccaa acttttcacg aatgtgatcg atctgccgct tgtcgccggt
      781 cagcagcttc cagttacggc cgtcgatacc gaaccgatcc gcgtactcgt tgagtaccgc
      841 caccgtatcg ttctgcggat cgatcgtgat cgagtagaaa tgcacgtcct caccgacgcg
      901 gtcgccgagc agccggtaaa cgtcggcaag ccgcgcggtc tccatcgggc aaatgtcggt
      961 gcagttggta aagatgaagt tgattgccac gaccttgccg gcgatcacat catcaaaaaa
     1021 ccgcagtttc tcgccggtgt gcgctgtcag ctcgtaattg gggaagtagt cggcacccca
     1081 tacctggccg cccgcgcgca caccgcccgc ctgcaaacag gcagccgccg ccagcagcag
     1141 cgccattgac caggcgcgga tcgacgcggg tccggcatgc ctgttgatcg aaatactcat
     1201 gtatgaatcc tcttgccggg gctgcccacc agccgctgcg cgactggtgg gcctgccgtt
     1261 gccggtcagg gttgcaggcc gaagccttca ccgctgcgga aagtcggcag cgcgatcgag
     1321 tcgacgtatt cgacaccgtc ccaggtgggg ataggcgcgg gcatgatctg cacctgtccg
     1381 ggttgctcca ggtcccagcg caacagcatc gcgtgatcct cgtgctgcgt gttatgacaa
     1441 tgctccacgt agctgccggc gaattcgcgg aagttgtaga acacctcgac ttcgcgtgca
     1501 gcgtcctctt cgggcccgag gcggaagacg tcctttcttg cccagcggtg ccactcgggc
     1561 ggcgtctcgc cgtcctcggt caggatcacg ccttcctcga agtgcacgtg aatcgggtgg
     1621 ctccagccac cgccggtttc gaagcgccag atctcggcgg tgccgtcacc agcgtagcca
     1681 ccaggcgtgg gcccgttcgc cagctgcggc gcggccgtca ggcggcgcgt gtcggcggta
     1741 taggcactgc cgccgtcggt cttgatcgtc cacggcgccg aatcggtgcc cgagctgcgg
     1801 ccgaagacga aagtccggcg gcgtgcatcg gcgacttcgg ccatctgcac gggatcgtcg
     1861 cgatcgatca ccaacgagat catcttttgc ccacccggca cataatccgc cgggttcatg
     1921 ctcaggtcgg tgccgccgta ggctttaatg cggaattcga gaaacttgcc aacgaccgga
     1981 tcaccatttt cccagcgagc cgccacacca tcgtcgtagt cgagctcggc cttgtaggtc
     2041 tcgcgcaata ccgcgccgag atcgacgctg ccctcgacca ctttgccgtt cttgtgctcg
     2101 agcaggttga cgaagtacag cttgtcaccc ggctgcagcc cgtgcgcagc gaaatcgacg
     2161 acgatgtcga agcgctcggc gatcgcctgc accggcagcg taccgcggtg ctcgtcggca
     2221 ttgccgtcgc cgtcgagatc gacactgccg tcgaacggta ccgtgtgctc catgatgttg
     2281 ccgtcgttgg caaccatgtg gaaaggaatg cggttccacg ataccccttg cgagcggcgc
     2341 cccggaattt caccactgcg gtgaccgacg acttcctgca ccagcgccag ctttaagtaa
     2401 cgcgataccg agccgttgag gatgcggaag cggtagcggc gcgcgcgcac atcgaagtac
     2461 ggcttgtaaa gccagttgac ggtcatctgg tcaccgagga atccatcctt gttgaagatg
     2521 ttgaaccaca actgaccctc gtcatcccag gccttggcgg cgagcagcag gttgacatcg
     2581 taatcgcggt tgccccatgg caacgcacta ccgctcggca ggcgcaggtt gacaccgtcg
     2641 tcgacggcct cgttaccacg atcgagcgcg ctgtagtaat tcatcatcgc agcgctgccc
     2701 ttgtagacgt tctgcgcggt gaaatcgagc atgtggtcgt ggaaccagtg cgtgctcatc
     2761 gtctcgcggt agtcacccgg aatccggatc gtgccgccgg cgccatccgg cgtggccgct
     2821 ctgggatcgg acgcgtcggt attgatgtga tcgtgtccgg caaggatcat cggccagc
//
LOCUS       DATCZV010000215         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_546491, whole genome shotgun sequence.
ACCESSION   DATCZV010000215 DATCZV010000000
VERSION     DATCZV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546491"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VIW27_04815"
     CDS             complement(<1..1136)
                     /locus_tag="VIW27_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739022.1"
                     /translation="MIAITVLSGLGLLRPAFNPLWIGGFAAWCAALLLFADTTRVLKI
                     QVSLILLAGIGLILFARSRGAAIDPDFVISSSTGLMTMIAAVGFLRLVVIPDTQQEES
                     LPVGRKALLQTLIGLNVSSSVINISAPILIADRIHRKRPIDRFTAQTFTRVFCGVSSW
                     SPFFGAMAVVLTYVSDASLAWIIVAGLPFMLIGFIGVYVEARLRYAREIEDFVGYPTQ
                     LRDLGVPAILIVVVLAMSRLLEGAPILVLIAISALLVTVVVLAVRVGARQSLTRLVAH
                     VQQGLPRIVNELCLFLSAGVLAAGISALVMHGVIDNPVTRFDAVSAAGLLALIMLCAF
                     CGIHPIIMISSLSPMILTLDPDPNLLAATYLFSWHIGTCSNPLSG"
     gene            complement(1282..2682)
                     /locus_tag="VIW27_04820"
     CDS             complement(1282..2682)
                     /locus_tag="VIW27_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5739023.1"
                     /translation="MSIDENQIAKMLRGVQILRKLDDEIIIDIARHIKTTAFQADQLI
                     VEKGRIGERIFFIFEGTVEVRIPGERAEEERRILLRKGEVVGEISLLINSSYSADIVA
                     LSSTRAFYLDKPRFEELVETHPDFAELMTQLMISRMAQKGGINRVGKYELRGKLGEGN
                     MATVFNAWDPELEREVAIKMLKYKLSYDEKFVQRFEREAKTIASLNHPNIVNVYEVIE
                     GFSTRFIVMEKLHGRDLSAMLDTKGAFSIAETRDILSQVASALQYAHHHGRNGIVHRD
                     IKPSNIVIDDYGNIKLTDFGIASPPQDKDVNIEGTPSYLAPEIINGDLLDGRADIYAL
                     GAMAFHMLTKSPPFSASTLSKLLMMQVQQKPPNIRNNIPDIDDAFADFIESALSKNPN
                     DRISDWERIRKTLMPLAGKFELPLDPHELAVIIRFRDTSYQQSASFINSMHKILQEQG
                     INHLIEMQRGRADDET"
     gene            complement(2775..>3128)
                     /locus_tag="VIW27_04825"
     CDS             complement(2775..>3128)
                     /locus_tag="VIW27_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5739024.1"
                     /translation="ATNADPGLALYNASKAFVQGLTRSIAVDHGPAIRCNAIAPGWIM
                     TGMADAAFALARDPEAARRDALHRHPAGRFGQPADIAALAAWLASDEAAFATGQCYTL
                     DGGLTAASPLNPGLF"
BASE COUNT          629 a          890 c         1014 g          595 t
ORIGIN      
        1 cccgacaacg gattcgaaca ggtgccgata tgccatgaaa acaggtaggt cgctgccagc
       61 agattgggat cggggtcgag cgtcagaatc atcggcgaca ggctcgagat catgatgatc
      121 gggtgaatgc cgcaaaatgc gcatagcatg atcagggcca gcaggccggc cgcgctgacg
      181 gcatcgaagc gtgtcaccgg gttgtcgatg acgccgtgca tgaccagcgc cgagatgccc
      241 gcggcgagta cgcccgccga caggaacagg cagagttcgt tgacgatgcg cggcaggccc
      301 tgctgcacgt gcgcgaccag tcgtgtcagg gattgccgcg ccccgacgcg aacggcgagc
      361 acgacgaccg tcaccagcag agcgctgatt gcgatcagta ccagaatcgg ggcaccctcg
      421 agcaggcgcg acatcgccag caccacgacg atcagaatcg ccggcacgcc gaggtctcga
      481 agctgggtcg ggtagccgac gaaatcctcg atctcgcgcg cgtaacgcag gcgtgcctcg
      541 acgtagacgc cgataaaacc gatcagcatg aatggcaagc cggcgacgat gatccatgcc
      601 aggctggcat cggatacata ggtcaacacc accgccatcg cgccgaagaa cggtgaccag
      661 ctggagacgc cgcagaatac tcgggtaaag gtttgcgcgg taaaccggtc gatcgggcgt
      721 ttgcggtgaa tgcggtcggc gatcaggatc ggcgccgaga tattgatcac cgagctcgac
      781 acgttcaagc cgatcagggt ttgcagcagc gccttgcgcc cgaccggcag cgattcttcc
      841 tgctgggtgt cgggaatcac caccaggcgc agaaagccga ccgcggcgat catcgtcatc
      901 aggccggtgc tcgaactgat gacgaaatcg ggatcgatgg cggcgccgcg gctgcgggcg
      961 aacagaatca ggccgatgcc ggcgagcagg atcagcgata cctgaatttt cagcacccgc
     1021 gtggtgtcgg caaacagcag cagcgcggcg caccaggcgg cgaatccgcc gatccacaac
     1081 gggttgaaag ccggtcgcag caggcccagc ccggagagta ccgtgatcgc gatcatcagc
     1141 agaaccagcg ggccggcaag ccgatggcga aacggggtat cggcgacgtt gatagcgaat
     1201 cctcggggcg ggtgggactg ccgccaatgt taaccgaaag ccgcccaaac gcttagcgcc
     1261 gtggccggcg cggatcgctt ttcaggtttc gtcgtcggcc ctgccgcgct gcatttcgat
     1321 cagatggttg atgccctgtt cctgcagtat tttgtgcatc gaattgatga agctggcgga
     1381 ttgctggtag gaggtgtcgc gaaagcgaat gatgaccgcc agttcgtggg gatccagcgg
     1441 taattcgaac ttgccggcca gcggcatcag ggtcttgcga atccgctccc agtccgagat
     1501 gcggtcgttg ggattcttgg acaatgcgct ctcgatgaaa tcggcgaacg catcgtcgat
     1561 atcgggaatg ttattgcgaa tattcggcgg cttctgctgg acctgcatca tcaacagctt
     1621 ggacagcgtc gaagcgctga acggtgggct cttggtcagc atgtgaaacg ccatcgcgcc
     1681 gagcgcatag atatcggcgc gcccgtcaag caggtcgccg ttgatgattt ccggggccag
     1741 ataggacggc gtgccctcga tgttgacatc cttgtcctgc ggcgggctgg cgatgccgaa
     1801 atcggtcagc ttgatgttgc catagtcgtc gatcacgata ttggagggct tgatgtcgcg
     1861 atgcacgatg ccgttcctgc cgtgatgatg tgcgtattgc agggcgctgg cgacctgcga
     1921 caggatatcc cgggtctcgg cgatgctgaa ggcgcctttg gtatccagca tcgccgacag
     1981 gtcgcgtccg tgcagttttt ccatgacgat gaatcgagtc gaaaatccct cgatgacttc
     2041 gtagacgttg acgatattgg ggtgattcag gctggcgata gtctttgcct cgcgctcgaa
     2101 gcgctgcacg aatttttcat cgtaggagag cttgtacttg agcatcttga tcgccacctc
     2161 gcgctccagc tcggggtccc aggcgttgaa cacggtcgcc atattgccct caccgagctt
     2221 gccgcgcagt tcgtatttgc cgacccggtt gatgccgccc ttttgcgcca tgcggctgat
     2281 catcagctgg gtcatcaact cggcgaaatc ggggtgggtt tcgaccagtt cctcgaaacg
     2341 cggcttgtcg agataaaatg ctcgagtact gctgagggcg acgatatcgg ccgaatagga
     2401 cgagttgatc aataacgaga tttcgccgac cacttcgccc tttctgagca ggatgcggcg
     2461 ctcttcttcg gcgcgttcgc cgggaatacg cacctcgacg gtaccttcga agataaaaaa
     2521 gatgcgctcg ccgatacggc ctttttcgac gatcagctgg tcggcctgga acgcggtcgt
     2581 cttgatgtgc cgggcgatgt cgatgatgat ctcgtcgtcc agcttgcgca gaatctggac
     2641 gcctcgcagc attttcgcga tctggttctc gtcgatggac atagggtaaa tcggcccggt
     2701 tttgggttat tcgactacag catagttcga ttcgcgcgcg aggtggcgct acaggtcaca
     2761 tatagtataa aaacctaaaa cagaccggga tttaacggcg aggcagcagt caggccgccg
     2821 tccagcgtat agcactggcc ggtggcgaaa gcggcttcgt cggaggccag ccaggcagcc
     2881 agcgcagcga tgtcggcggg ctggccgaag cgaccggccg ggtggcggtg cagggcgtcg
     2941 cgccgcgccg cctcggggtc tcgcgccagc gcgaacgcgg cgtccgccat gccggtcatg
     3001 atccaccccg gagcgatcgc gttacagcgg atcgcgggac catgatcgac ggcgatcgag
     3061 cgggtcaggc cctgcacgaa ggccttggaa gcgttataca gagcgagccc gggatcggcg
     3121 ttggttgc
//
LOCUS       DATCZV010000216         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_228836, whole genome shotgun sequence.
ACCESSION   DATCZV010000216 DATCZV010000000
VERSION     DATCZV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228836"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1603)
                     /gene="betC"
                     /locus_tag="VIW27_04830"
     CDS             complement(71..1603)
                     /gene="betC"
                     /locus_tag="VIW27_04830"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004545874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HEY5739025.1"
                     /translation="MENILIVMADQLSALALGCYRNPAVKSPNIDRLADEGVLFESAY
                     SSSPLCTPARYAFMTGQNISVCGGYDNAAYMPATMPTFAHYLRLMGYRTCLSGKMHFV
                     GPDQLHGFEDRVTTDIYPADFGWVPDWNHPDERIDLWYHNMSSVKQAGVAAITNQLAY
                     DDEVGSQAMRVIYDHARSEDQRPLCLVASFIHPHDPYATRQQYWDLYADVAIPLPAVP
                     RPAEQDPHHKRLEKVIALDAVDVSDQEIINARRAYYGNVSYVDEWLGRLRATLDDCGM
                     GDNTTILLTSDHGDMLGEFGLWYKMSFREWSNRIPLIVHRPSRFASRRVAAPVAQVDV
                     LPTLVDLAAEATGLDKPMPIDPLHGRSLIPLCDGDASGDPNACVSEYLAEGTGAPMLM
                     IRRGQYKYISCSTDPEQLFDLEADPHELENLADHPQLESFRAAAAAHWDSEAVRAAVI
                     HSQERRRAVHAALRHGRYQGWDFNPPRDASEEYTRSHMDLTKFDITSRYPRPPAFNPK
                     WK"
     gene            complement(1632..1991)
                     /locus_tag="VIW27_04835"
     CDS             complement(1632..1991)
                     /locus_tag="VIW27_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY5739026.1"
                     /translation="MTHHYEGSCHCGAVKFSYDGEEITRGLRCTCSMCRRKGAMMSTE
                     AIPQENLKVEADPDDLGLYQFDSKIAKHYFCKRCGIYTHNVMLRFPDKCRVNLGCIDD
                     LDTSGFEVDVFDGKNLI"
     gene            complement(2435..>2800)
                     /locus_tag="VIW27_04840"
     CDS             complement(2435..>2800)
                     /locus_tag="VIW27_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739027.1"
                     /translation="LETDWTGAILNSHDQALTRRIFDAWDLACRNPNLPKRLRPLLTG
                     LGFAALRVEAIPVLNAGYCENSFSARMLENFAVAARKRQIITADESRAWLDGIAALIR
                     DDAYFFCVNRFLFTAIKQH"
BASE COUNT          482 a          809 c          956 g          553 t
ORIGIN      
        1 tcccggaaat ttagatctga cccgaattaa agcgcatcgt ttaatttaga tctgacccaa
       61 attaagtggg ttatttccat ttggggttga aggccggcgg gcgcgggtag cgcgaggtga
      121 tgtcgaattt cgtcaggtcc atgtggctgc gcgtgtattc ctccgaagcg tcgcgcggcg
      181 ggttgaaatc ccagccctgg tagcgaccat ggcgcagcgc cgcgtgcacc gcgcggcggc
      241 gttcctggct atggattacg gcagcgcgca ccgcctcgct gtcccagtgg gccgcggccg
      301 cggcgcgaaa cgattccagc tgcgggtgat cggccaggtt ttcgagctcg tgcggatcgg
      361 cctcgagatc gaacagttgc tcggggtcgg tgctgcagct gatgtacttg tactgcccgc
      421 ggcggatcat cagcatcggc gcaccggtgc cctcggcgag gtactcgctg acgcaggcgt
      481 tcgggtcgcc gctcgcatca ccgtcgcaca acgggatcaa cgagcgcccg tgcagcggat
      541 cgatcggcat cggcttgtcg agtccggtgg cctcggcggc aagatcgacc agcgtcggca
      601 gtacgtccac ctgggccacc ggtgcggcca cgcggcgcga cgcgaagcgc gatggccgat
      661 gcacgatcag cggaatgcgg ttcgaccatt cgcggaaact catcttgtac cagaggccga
      721 actcgccgag catatcgccg tgatccgagg tcagcaggat ggtcgtattg tcgcccatgc
      781 cgcaatcgtc gagagtcgcg cgcagccggc cgagccactc gtcgacatag ctgacgttgc
      841 cgtagtaggc gcggcgcgcg ttgatgattt cctggtcgct gacatcgacc gcgtcgagcg
      901 cgatgacttt ttccagccgc ttgtggtgcg ggtcctgttc ggccggccgc ggcaccgccg
      961 gcagcggtat ggcgacatcc gcgtaaagat cccagtactg ctggcgcgtg gcgtagggat
     1021 cgtgcgggtg gataaaactc gccaccaggc acagcggccg ctggtcctcg ctgcgcgcgt
     1081 ggtcgtagat cacgcgcatc gcctggctgc cgacctcgtc gtcgtaggcg agctggttgg
     1141 tgatcgccgc gaccccggcc tgcttgacgc tcgacatgtt gtggtaccac aggtcgatac
     1201 gctcgtcggg atggttccag tccggcaccc agccgaaatc ggccgggtaa atatcggtgg
     1261 tgacgcgatc ctcgaagccg tgcaactggt ccgggccgac gaagtgcatc ttgcccgaca
     1321 ggcaggtgcg gtatcccatc aaccgcaggt aatgggcaaa ggtcggcatg gtcgccggca
     1381 tgtaggcagc gttatcgtag cccccgcaaa cggaaatgtt ttgcccggtc atgaacgcgt
     1441 agcgcgccgg ggtgcacagc ggactcgaac tgtaggccga ttcgaacagt acgccctcgt
     1501 cggcgaggcg gtcgatgttg gggcttttca ccgccgggtt acggtagcag ccgagcgcca
     1561 gcgcgctgag ctggtcggcc atcacgatca ggatgttttc cattgcggtc ggctcgctcg
     1621 aggtaaccgg gctagatgag attcttgccg tcgaagacgt cgacctcgaa acccgaggta
     1681 tcgagatcgt cgatgcagcc gaggttcacc cggcacttgt cgggaaagcg cagcatcacg
     1741 ttgtgggtat aaatgccgca acgcttgcag aaatagtgtt tggcgatctt cgagtcgaac
     1801 tggtaaagcc cgagatcgtc cgggtcggcc tcgaccttga ggttttcctg cggaatcgct
     1861 tccgtgctca tcatcgcgcc cttgcgccgg cacatcgagc aggtgcaacg caagccgcgg
     1921 gtgatttctt cgccatcgta ggaaaatttg accgcgccgc aatggcagct gccctcgtag
     1981 tggtgtgtca tgtcgtctcc ttcggattgg atcgagcgct cgatcaaaac ggctagtttg
     2041 ttcttacgtc gccgtcattt caaggttatc gcgggcacgg aggtatgtta cgcatttaat
     2101 agccgcagct gacggcaagc ccgcgggctt gccgtgcgga ctctgccggt acggtgcgat
     2161 gggccccggc tcgggggccg gggcgacggc ggcgcgggga tgacgcgggg ggtcgctgac
     2221 ctcgggagat ccccgcttgt gcgacgatga cgagtagtgg tggggatgat gggtagtagc
     2281 gggatgaggt atttgtgagt cattcccacg taagcggtgc accgccccgc ggtaatcttg
     2341 cactaacgcc gcggttacaa gatctccgct tgtgccggga tgacgcgggg ctcgctgacg
     2401 ctgggagata cccgcttgcg cggggacggc gctgttagtg ctgtttgatg gcggtgaaca
     2461 ggaagcgatt gacgcagaag aaatacgcgt cgtcgcgaat cagcgcggcg atgccatcca
     2521 gccaggcgcg ggattcgtcg gcggtgatga tttgccgctt gcgtgccgcc acagcgaagt
     2581 tttccagcat gcgggcggag aagctgtttt cgcaataacc ggcattgagc accggtatcg
     2641 cctcgacgcg cagcgcagcg aaaccgaggc cggtgagcag cgggcgcagg cgtttgggca
     2701 ggttcggatt cctgcaggcg aggtcccagg cgtcaaagat gcgccgcgtc agcgcctggt
     2761 cgtggctgtt gaggatggcg ccggtccagt cggtttccag
//
LOCUS       DATCZV010000217         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_652368, whole genome shotgun sequence.
ACCESSION   DATCZV010000217 DATCZV010000000
VERSION     DATCZV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652368"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VIW27_04845"
     CDS             complement(<1..988)
                     /locus_tag="VIW27_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY5739028.1"
                     /translation="MADRKFGIETLCLHAGQVPDPVTGARAMPIYQTTSYVFDDAEHA
                     ASLFNLQTFGNIYVRLSNPTTAAFEERMAALENGRAGLAVSSGMAAQMTAILTLMEQG
                     DHLVSAKALYGGTFSQFDYTFRKMGIDTTFVDSDVPENFEKAITPKTKALYAEIIGNP
                     TNNILDIEAVGAIAAEHGIPLIVDNTFATPYLCKPMDHGADIVVHSATKFVGGHGTSM
                     GGVLVESGRFDYGNGNFPGMTEPSEGYHGIRFYETFGDFGFTGKARCETLRTLGQALS
                     PFNAFMLLQGLETLPVRMDRHCQNALGVARFLRDHPAVEWVNYAGLEDNPYRD"
     gene            complement(1001..1480)
                     /locus_tag="VIW27_04850"
     CDS             complement(1001..1480)
                     /locus_tag="VIW27_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY5739029.1"
                     /translation="MTDDTDIRTLRRILHDYRRVAMVGLSADWYRPSNFAAKYLLDRD
                     FEVIPVNPKYDEILGQKCYPDLVSIPTPVDIVDLFQRPDRIPPFVDQAIEIGAKVVWM
                     QLGIVNQEAAQKARDAGLEVVMDRCMKIEYARLYGGLNWLGVNTGVISAQRPRYINR"
     gene            complement(1477..>2826)
                     /locus_tag="VIW27_04855"
     CDS             complement(1477..>2826)
                     /locus_tag="VIW27_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5739030.1"
                     /translation="GAVIHALNFRLDAAAIAFQLEHGGARVLLTDREFSATIAAALEQ
                     LETKPYVIDVDDAAAEDGELIGDIEYEAFIAGGDLDFDWRPIDDEWRAIALGYTSGTT
                     GNPKGVVTHHRGAYLNAISNVLAWNLQAHPVYLWTLPMFHCNGWCFPWTLAALNGTSV
                     CLRAVRDDAIYDAIKRHRVTHLCGAPVVLSLLVNADEQLKAGIDHRVEVMTAAAPPPP
                     AILEAMANLGFEVTHVYGLTEVYGPAVVCSWHREWNQLSYAEQAEVKSRQGVRYPMLE
                     ELVVMDPETMLPVPRDGETLGEIMFRGNIVMKGYLKNPQATGEAFAGGYFHSGDLAVC
                     HPDGYIEIRDRAKDIIISGGENISSIEVEQVLYRHPAVLEAAVAAMPDDKWGETPCAF
                     VTLRADAGDVGEREIIDFCRERMARFKAPRKVVFGPLPKTSTGKIQKFELRERARQL"
BASE COUNT          517 a          849 c          916 g          544 t
ORIGIN      
        1 ggtcgcggta ggggttgtct tcgaggccgg cgtaattgac ccactcgaca gccgggtgat
       61 cgcgcaggaa tcgggccacg ccgagcgcgt tttggcaatg gcggtccatg cgcaccggca
      121 gggtttcaag cccttgcagc agcatgaaag cattgaacgg gctcagcgcc tggccgagcg
      181 tgcgcaacgt ttcgcagcgg gccttgccgg tgaagccgaa atcgccaaag gtctcgtaga
      241 aacggatacc gtggtaaccc tccgagggtt ccgtcatgcc cgggaaattg ccgttgccat
      301 agtcgaagcg gccggattcg accagcacgc cgcccatact ggtgccatga ccgccgacga
      361 acttggtcgc cgagtgcacg acgatatcgg ccccgtggtc catcggcttg cacaggtagg
      421 gggtcgcgaa ggtattgtcg acgattaacg gaatgccgtg ctcggcagcg attgcgccga
      481 ctgcctcgat atcgaggatg ttgttggtcg ggttgccgat gatttccgcg tacagggcct
      541 tggtcttcgg tgtgatcgcc ttctcgaaat tttccggtac gtcggaatcg acgaaagtcg
      601 tgtcgatgcc catcttgcga aacgtgtagt caaactgcga aaaggtccct ccgtagaggg
      661 ccttggccga caccaggtga tcgccctgct ccatcagcgt caggatcgcg gtcatctgcg
      721 cggccatgcc ggagctgacc gcgagtcccg cgcggccgtt ctcgagtgcc gccatgcgct
      781 cctcgaaagc cgccgtggtg gggttggaca ggcgcacgta gatattgcca aaagtctgca
      841 ggttgaacag gctggcagcg tgctcggcat cgtcgaacac gtaggaagtc gtctggtaga
      901 tcggcatcgc acgcgcgccg gtcaccgggt cggggacctg cccggcgtgc aggcacaggg
      961 tttcgatacc gaattttctg tctgccatga tctgctccgg ttaccggttg atgtagcgcg
     1021 ggcgttgcgc ggagatcacg ccggtattga cgccgagcca gttgagcccg ccgtaaagac
     1081 gcgcgtactc gattttcatg caacgatcca tgaccacttc gagcccggcg tcgcgcgcct
     1141 tttgcgcggc ttcttggttg acgatgccga gctgcatcca gaccactttg gcgccgatct
     1201 cgatcgcctg gtcgacgaac ggtggaatac gatccgggcg ctgaaacagg tcgacgatgt
     1261 cgaccggggt cggaatcgag acgaggtcgg gataacactt ttgccccagg atttcgtcgt
     1321 atttcggatt taccgggatg acttcaaaat cgcggtcgag caggtacttg gcggcgaagt
     1381 tactgggtcg ataccagtcg gcggaaagcc cgaccatggc gacgcgccgg tagtcatgaa
     1441 gaatacgccg cagcgtcctg atatcggtat cgtcggtcat aattgcctcg cacgctcgcg
     1501 cagttcgaat ttctggatct tgccggtcga ggttttcggc aacgggccga acaccacctt
     1561 gcgtggtgcc ttaaagcgcg ccatgcgctc gcggcagaag tcgatgatct cccgttcgcc
     1621 gacgtcgccg gcatcggccc gcagggtcac gaaggcgcac ggcgtctcgc cccatttgtc
     1681 atcaggcata gccgcaaccg cggcttcgag cactgcgggg tgtcgataca gcacctgctc
     1741 gacctcgatg ctggaaatat tctcgccacc ggaaatgatg atgtccttgg cgcgatcacg
     1801 aatttcgata taaccgtccg ggtggcagac cgcgagatcg ccggaatgga agtagccgcc
     1861 ggcgaaagcc tcgccggtcg cctgtgggtt tttcaggtaa cccttcatca cgatattgcc
     1921 gcgaaacatg atttcgccga gagtctcgcc gtcgcgcggt accggcagca tggtctcggg
     1981 gtccatcacc acgagctcct cgagcatcgg gtagcgcacg ccctggcgcg acttgacctc
     2041 ggcctgctcg gcataggaca gctggttcca ttcgcgatgc cacgagcaga ccaccgccgg
     2101 cccatagacc tcggtcaggc cgtagacgtg ggtgacttcg aaaccgagat ttgccatcgc
     2161 ctcgaggatc gccggcggcg gcggcgcggc cgcggtcatg acttcgacgc ggtgatcgat
     2221 acccgccttg agctgctcgt cggcgttgac cagcaggctc agcaccaccg gtgcgccgca
     2281 cagatgcgtg acgcggtgac gcttgatggc atcgtaaatc gcatcgtcgc gcaccgcgcg
     2341 caggcagacg ctggtaccgt tgagcgccgc cagcgtccac ggaaagcacc aaccgttgca
     2401 gtgaaacatc ggcagcgtcc acaggtaaac cggatgggcc tgcaagttcc aggcgagcac
     2461 gttggaaatc gcgttgagat aagcgccgcg atgatgggtg accacgccct tggggttgcc
     2521 ggtggtgccg gaggtgtagc cgagcgcgat cgcgcgccat tcgtcgtcga tcggccgcca
     2581 gtcgaagtcg agatcgccgc cggcgatgaa tgcctcgtac tcgatatcgc cgatcagctc
     2641 gccgtcttcg gccgccgcgt cgtcgacgtc gatgacgtag ggcttggttt cgagctgctc
     2701 gagcgcggcg gcgatggtgg ccgaaaactc gcggtcggtc agcagcacgc gggcgccgcc
     2761 gtgctcgagt tgaaaggcga tcgcggccgc gtcgagacgg aaattgagcg cgtgaatcac
     2821 ggcgcc
//
LOCUS       DATCZV010000218         5596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_705417, whole genome shotgun sequence.
ACCESSION   DATCZV010000218 DATCZV010000000
VERSION     DATCZV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705417"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="VIW27_04860"
     CDS             <1..1687
                     /locus_tag="VIW27_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5739031.1"
                     /translation="NANTLVLVNGRRLNNADLDPPALNHILPADIERIEVMVGSAGSL
                     YGDQAVGGVVNIITRRPAEPDQRVELRAGSYDYRGVQFSSSMQVNESFGYRLSAETFD
                     TNNYRDHNAQQYSNVGTLLDYQRGANRLFAELQAISDDLELPGALLEDEFEDDPQQIN
                     PAFENDFRDEDTRVYRVGYERELGAQQLSIDSSRRKTESDLLQSFRNSPSPAAGFLDR
                     ENGSVNAKLAGSFEFGYATSYVIGIDIEETELEFEIPNSFSSSRSSSEQDTDSLYFQL
                     NPRLSQDLQLTLGMRHSRVENDLDYADVLGTQTQDKFDDQVTVGELGLVYRIDDGTRA
                     TLRYDQNFRFAKVDELTFSTEAGTLLDTQTGESWEIGLDFVRGSQRLLVSVYQLDLEG
                     EIAFDPTVGPDFGFGPTGLNVNFDETRRRGATLSWQSSLSRQLSLNTEISLVDAEFKS
                     GVFEGKNISGVADQLASLRADYQLSGAAGIYLEYNYTSPRYAQGDNANELGKLASITV
                     YNAGAGYRIAGWDLALRINNLADESYAEFINSFGAVFPSPERNYMLTAGYRFE"
     gene            1687..2250
                     /locus_tag="VIW27_04865"
     CDS             1687..2250
                     /locus_tag="VIW27_04865"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY5739032.1"
                     /translation="MGNRLSKIYTRTGDDGTTGLGDGSQRSKDDPRVQCMGEVDELNA
                     SIGLALSMMEDGPLQQALFAVQHDLFDIGAELAQPGKVLLDDAYVAALERRADEFNAE
                     LAPLKEFILPGGPQSVAAIHLARTICRRVERGLVGLAAIEDVNPATGRYLNRLSDLLF
                     IIGRAQSQGEDGGEVYWNSKYSRLNRG"
     gene            complement(2247..3038)
                     /locus_tag="VIW27_04870"
     CDS             complement(2247..3038)
                     /locus_tag="VIW27_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5739033.1"
                     /translation="MPQLGYQRLGDGPPLLILHGLFGSGRNWQSHARRLAEFFDVVTI
                     DLRNHGQSFHADEMDYSLMAADVERLIGDLDLGACNLLGHSMGGKVAMALALESPQTV
                     ARLVVADIAPVIYRHDHDELVDAALGLAVDAIGARADADRELQSAVPDPGLRAFLLQN
                     LVRDGDNWRWRVNWRAIQRNMRQLTGFVELADDWHVDVPALFIRGAKSDYVGAAEIEQ
                     IERHFGNAEIATVDDAGHWLHAERPQAFIERVLEFLLPPAERGGD"
     gene            complement(3063..4805)
                     /gene="lpdA"
                     /locus_tag="VIW27_04875"
     CDS             complement(3063..4805)
                     /gene="lpdA"
                     /locus_tag="VIW27_04875"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY5739034.1"
                     /translation="MSVKDLLVPDIGDFEKVEIIELLVAEGDTIEVEDPILTLESDKA
                     TMDIPSPYAGTVKTLGVAVGDKISEGDKLGSIEVAGGSAAGGETAGKASEKAATSEVP
                     AAAKPAGDADVEAEVVVLGAGPGGYTAAFRAADLGKKVVLVERYESLGGVCLNVGCIP
                     SKALLHTAQIINEAAHMQANGVTFGKPKIDLKGINGFKDKVVGKLTGGLAGLAKQRKV
                     KVVHGVAQFTGPNNLQVEKDGKTTNISFQNCIIAAGSQATEIPSFPNDDPRLMDSTDA
                     LLLEEVPKKLLVVGGGIIGLEMATVYHALGSEISVVEFMDTLIPGCDKDLLRGLNKII
                     SKRYKAIYLKTRVSEIKAQKNGLKVTFEGDKAPDPAIYDRVLVAVGRRPNGHKIGADK
                     AGVKVDDAGFIPVDKQMRTNVPHIFAIGDIVGNPMLAHKATHEGKTAAEVIAGMKSYF
                     EPLTIPSVAYTDPEVAWMGLSEAEAKEQGIDYEKGAFPWAASGRSLGIDRDEGMTKVI
                     FDKQTKRILGAGMVGPNAGELIAEAVLALEMGADAEDIGLTIHPHPTLSETFNFAAEM
                     AEGTITDLYVPRKK"
     gene            complement(4808..>5596)
                     /locus_tag="VIW27_04880"
     CDS             complement(4808..>5596)
                     /locus_tag="VIW27_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY5739035.1"
                     /translation="VKSAMAASTGGAAAAGGVQVSSAPAVDHSKFGEIETVPLSRIKK
                     ISGAHLHRSWVTVPHVTQFDEADITELEAFRKSLAKEAEKKTVRMTPLIFLMKAVVCA
                     LREYPDFNASLDADGENLIMKKYFHIGIAVDTPNGLVVPVVRDVDQKGLFDLAGELGE
                     ISARARDGKLSPTEMQGGCFTISSLGGIGGTYFTPIVNAPEVAILGVSRSKMQPVYID
                     GEFQPRLILPLGLSYDHRVIDGAQAARFTSFLSQVIADIRRILL"
BASE COUNT         1044 a         1779 c         1708 g         1065 t
ORIGIN      
        1 caacgccaac acgctggtgc tggtcaacgg tcggcggctt aacaatgccg atctcgatcc
       61 gccggcgctg aaccacattc ttccggcgga catcgaacgc atagaagtaa tggtgggcag
      121 cgccggcagc ctctatggcg accaggcggt cggcggcgtg gtcaatatca tcacccggcg
      181 cccggccgaa cccgatcagc gtgtcgagct gcgcgccggc agctacgatt accgtggagt
      241 gcagttcagt tcgtcgatgc aggtaaacga atccttcggc taccggttat ccgccgagac
      301 cttcgacacc aataattacc gcgatcacaa cgcgcagcag tattccaacg tcggcactct
      361 actcgactac caacgcggcg cgaaccggct gttcgccgaa ttgcaggcca tcagcgacga
      421 tctcgaattg ccgggtgcgt tgctcgagga cgagttcgaa gacgatccgc agcaaatcaa
      481 cccggccttc gagaacgact ttcgcgacga ggatacgcgc gtctaccggg tcggctacga
      541 gcgcgaactc ggtgcccagc aattgagcat cgacagttcg cgacgcaaga ccgagagcga
      601 tctgttgcag agtttccgca atagcccgag cccggccgcg gggttcctcg accgcgagaa
      661 cggcagcgtc aacgccaagc tcgcgggcag cttcgagttc ggctacgcga ccagctatgt
      721 gatcggtatc gatatcgagg agaccgagct cgaattcgag ataccgaact cgttcagcag
      781 ctcccggtcg agcagtgagc aggataccga cagcttgtat ttccagctca atccgcgtct
      841 cagccaggac ctgcagctga cgctggggat gcggcattcg cgggtcgaaa acgatctcga
      901 ttatgccgac gtgctcggca cccagaccca ggataagttc gacgaccagg tcaccgttgg
      961 cgaactgggg ctggtttacc gaatcgacga cggcactcgc gcgacgttgc gctacgatca
     1021 gaactttcgc ttcgccaagg tcgacgagct gacgttctcg accgaggccg gcaccctgct
     1081 cgatacgcag accggtgagt cctgggagat cgggctggac ttcgtgcgcg gcagtcaacg
     1141 cttgctggtc tcggtctacc agctcgacct cgagggcgaa atcgccttcg atccgaccgt
     1201 cggccccgat ttcggattcg gccccaccgg gctgaacgtc aatttcgacg agacccgcag
     1261 acgtggcgcg accctgtcct ggcagagcag cctgtcgcga caactgtcgc tgaacaccga
     1321 gatcagcctg gtcgacgccg agttcaaatc cggtgttttc gagggcaaga atatttccgg
     1381 cgtggcggat cagctcgcca gcctgcgggc agactaccag cttagcggtg cggccgggat
     1441 ctacctggaa tacaattaca cctcgccccg ctatgcgcag ggcgacaatg ccaacgagct
     1501 tggcaagctc gcttcgatta cggtttataa tgccggggcc gggtatcgaa tagccggctg
     1561 ggatttggct ctgaggatca ataacctggc cgacgagagt tacgccgagt tcatcaacag
     1621 tttcggtgcc gtgtttccga gccccgagcg caactacatg ctgaccgcgg gataccgctt
     1681 cgaataatgg gaaatcgact gagcaagatc tatacacgca ccggcgacga tggcaccacc
     1741 ggcctcggcg acggcagcca gcgttcgaaa gacgatccgc gcgtgcagtg catgggcgag
     1801 gtcgacgaac tcaacgccag catcggcctg gcgctgagta tgatggaaga cggacccttg
     1861 cagcaggcgt tgttcgcggt acagcacgac ctgttcgata tcggcgccga actggcgcag
     1921 cccggcaagg tcctgctcga cgacgcctac gtcgccgctc tcgaacgccg cgccgacgag
     1981 ttcaacgccg agctcgcccc gctgaaggaa ttcatcctgc cgggcgggcc gcagtcggtg
     2041 gccgcgattc acctcgcgcg cacgatctgc cgccgcgtcg aacgcggact ggtcggcctg
     2101 gcggcaatcg aggatgtcaa cccggccacc ggccgatacc tcaaccggct gtccgacctg
     2161 ctgttcatca tcgggcgcgc gcagtcgcag ggcgaggacg gcggcgaggt gtactggaac
     2221 tcgaagtact cgcggctgaa ccgcggctag tcgccgccgc gttcggctgg cggcagtaaa
     2281 aattccagca cgcgctcgat gaaggcctgc ggccgctcgg cgtgcagcca gtgccccgca
     2341 tcgtcgacgg tagcgatttc ggcgttgccg aaatggcgct cgatctgctc gatctcggca
     2401 gcgccgacat aatccgactt cgcgccgcgg atgaacaacg ccggcacatc gacgtgccag
     2461 tcgtcggcca gctcgacaaa acccgtcagc tgtcgcatgt tacgctgaat ggcgcgccag
     2521 ttgacacgcc agcgccagtt atcgccgtcg cgcaccaggt tctgcagcag gaaggcgcgc
     2581 aggccgggat cgggaaccgc ggattgcagc tcgcggtcgg cgtcggcacg cgcgccgatg
     2641 gcgtcgacgg ccaggccgag cgcggcatcg acgagttcgt cgtgatcgtg cctgtagatc
     2701 accggcgcga tatccgcaac caccagcctg gcaaccgttt gcggggactc gagcgccagt
     2761 gccatcgcca ccttgccgcc catgctgtga cccagcaggt tacaggcgcc gagatcgaga
     2821 tcgccgatca gtcgttcgac gtcggcagcc atcagcgagt aatccatttc gtcggcatgg
     2881 aaggattggc cgtgattgcg caggtcgata gtgaccacat cgaagaattc ggccagccga
     2941 cgggcatgcg actgccagtt acggccggag ccgaacaggc cgtgcaggat cagtaacggc
     3001 ggcccgtcgc cgaggcgctg gtagccgagt tgcggcatgc cgttgagcgc ggctccgggg
     3061 ccttacttct tgcgcggcac gtagaggtcg gtgatcgtgc cctcggccat ttccgccgcg
     3121 aaattgaagg tttccgaaag tgtcgggtgc ggatgaatgg tcagaccgat gtcctcggcg
     3181 tcggctccca tttccagcgc cagcaccgcc tcggcaatca gttcgccggc attgggcccg
     3241 accatgccgg cgccgagaat gcgtttcgtc tgcttgtcga aaatcacctt ggtcatgccc
     3301 tcgtcgcgat cgataccgag cgagcggccg ctcgccgccc acgggaaagc tcccttctcg
     3361 taatcgatgc cctgctcctt ggcctcggcc tcactcaggc ccatccaggc gacctcgggg
     3421 tcggtgtagg cgaccgacgg gatcgtcagc ggctcgaaat aggatttcat gccggcgatg
     3481 acctcggccg cggtcttgcc ctcgtgggtc gccttatgcg cgagcatcgg gttgccgacg
     3541 atatcgccga tcgcgaagat atgcggcacg ttggtgcgca tctgcttgtc gaccggtatg
     3601 aaaccggcat cgtcgacctt gacgccagcc ttgtcggcgc cgatcttgtg gccgttggga
     3661 cggcggccaa cggccaccag cacgcgatcg tagattgccg gatcgggcgc cttgtcaccc
     3721 tcgaaggtga ccttgagacc gttcttctgc gccttgattt ccgacaccct ggtcttcagg
     3781 taaatcgcct tgtagcgttt ggaaataatc ttgttcaatc cgcgcagcaa gtccttgtcg
     3841 cagcccggga tcagcgtatc catgaattcc acgacgctaa tttcagagcc gagcgcatga
     3901 tagacggtgg ccatctcgag accgatgatg ccgccgccga ccaccagcaa cttcttcggt
     3961 acctcctcga gcaacagcgc atcggtggaa tccatcagcc gcgggtcgtc gttcggaaac
     4021 gacgggatct cggtggcctg cgagccggcg gcaatgatgc agttctgaaa cgatatattg
     4081 gtggtcttgc catctttttc gacctgcagg ttgttggggc cggtgaattg cgcgacgccg
     4141 tgcacgacct tgaccttgcg ctgcttggcg agcccggcga gtccgccggt gagcttgccg
     4201 actaccttgt ccttgaagcc attgataccc ttgaggtcga ttttcggctt gccgaaggtt
     4261 acgccgttgg cctgcatgtg cgcggcttca ttgatgattt gcgccgtatg cagcagcgcc
     4321 ttggatggaa tgcagccgac gttcaggcag acgccgccga gcgactcgta gcgctcgacc
     4381 agcaccacct tcttgccgag atcggcggcg cgaaacgcgg ccgtgtaacc gccgggtccg
     4441 gcaccgagta ccacgacctc ggcctcgaca tccgcgtcgc cggctggctt ggcggccgcc
     4501 gggacctcgc tggtggccgc tttttcggac gccttgccgg cagtctcgcc gccggccgcg
     4561 gaaccgccgg cgacctctat gctgccgagc ttgtcgccct cggaaatctt gtcgccaacg
     4621 gcgacaccga gtgttttcac ggtaccggcg taaggcgacg ggatatccat cgtcgccttg
     4681 tccgactcca gcgtcaggat cgggtcctcg acctcgatcg tatcgccctc ggcgacgagt
     4741 agttcgatga tctcgacttt ctcgaaatcg ccgatgtccg gaactagcag atcttttacg
     4801 ctcatgatta caataatatc cttctgatgt cagctattac ctggctcagg aaactggtga
     4861 agcgcgccgc ctgtgcgccg tcgatgacgc ggtgatcata ggacagcccc agcggcagta
     4921 tcagccgcgg ctgaaactcg ccgtcgatat acaccggctg catcttcgag cgcgacacgc
     4981 cgagtatcgc cacctcgggc gcattgacga tcggcgtaaa ataggtgccg ccgataccgc
     5041 ccagactgga aatcgtgaag cagccgccct gcatctcggt cggcgacaat ttgccgtcgc
     5101 gcgcccgcgc cgagatctcg ccgagttcgc cggcgagatc gaataatcct ttttgatcca
     5161 cgtcgcgtac caccggcacc accagaccgt tgggagtatc cacggcgata ccgatgtgga
     5221 aatacttttt catgatcagg ttttcgccgt cggcatccag tgaggcgttg aaatcgggat
     5281 actcgcgcaa cgcgcagacc accgctttca tcaggaaaat cagcggcgtc atacgcaccg
     5341 tttttttctc ggcctctttg gccagcgatt tgcgaaaggc ttccagctcg gtgatgtcgg
     5401 cctcatcgaa ctgcgtcacg tgcggtaccg tcacccagct gcggtgcagg tgcgcgcccg
     5461 aaatcttctt gatccgcgac agcggcacgg tctcgatttc gccgaacttc gagtggtcga
     5521 cggcgggcgc gctgctgacc tgtacaccgc cggcagcggc ggcgccgcca gtactggcgg
     5581 ccatcgccga tttgac
//
LOCUS       DATCZV010000219         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_387436, whole genome shotgun sequence.
ACCESSION   DATCZV010000219 DATCZV010000000
VERSION     DATCZV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387436"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /gene="paaZ"
                     /locus_tag="VIW27_04885"
     CDS             complement(<1..1579)
                     /gene="paaZ"
                     /locus_tag="VIW27_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEY5739036.1"
                     /translation="MNLQNFAENSWFTAADEGKVLTSAVDGQPVASITSSGLDFGAML
                     DYARRSGGSNLRKYTFHERALMLKELAKYLMEHKKEFYALSTQTGATRADSWIDIDGG
                     ISTLFVYSSKGRREMPNDKVYLDGAPEMLSRNGTFVGQHIYTPRPGAAVHINAFNFPC
                     WGMLEKLAPALLAGVPAIVKPASSTAYLTELVFRRMIESQILPEGSLQLICGGVGDLF
                     DHLTCQDIVAFTGSKSTAEKLQQHPRIVAESVHFTAETDSLNASILGPDATPDTPEFD
                     LFIKEVTREMTSKVGQKCTAIRRIIAPAELAADLVKALSTALGEIRIGNPASKEVDMG
                     ALASLGQREEVRARVADLSGEAEVVYGNRSDFDLVDADADRGAFFMPTLLHCERPLSA
                     TAVHSVEAFGPVSTVLPYNNLDEAIELARLGDGSLAGSVITNDNDIARQLVLGAAAWH
                     GRMLVINRHCAAESTGHGSPLPHLVHGGPGRAGGGEELGGIRGALHYMQRTALQGSPE
                     TLAAIGNRWIRGGAEATS"
     gene            1690..>2285
                     /locus_tag="VIW27_04890"
     CDS             1690..>2285
                     /locus_tag="VIW27_04890"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HEY5739037.1"
                     /translation="MSIQAASRALLDEFRSRPTIRAGSLIITVFGDAVAPRGGTVWIG
                     SLIKVLDDFGVSERLVRTSVFRLTRDAWLDVNQVGRRSYYSLTADGARRFEQATQRIY
                     GEPRQSWSGDWCLVLLADLAAEQKDIVRRELGWQGFAAISTNVLAHPSQPKPEIERTL
                     EGLGVERDLVVMQGRTLGKNQDEAMRTLVHKSWNLDDI"
BASE COUNT          424 a          696 c          730 g          435 t
ORIGIN      
        1 cggatgtagc ctcggcaccg ccacggatcc agcgattgcc gatcgcggca agcgtctccg
       61 gcgatccctg tagcgcggtt ctctgcatgt agtgcagcgc gccgcgaatg ccgcccagtt
      121 cctcgccacc accggcgcgg cccggaccgc cgtggacgag gtgcggcaac ggggaaccgt
      181 gcccggtcga ttccgcggcg caatgacgat tgatgaccag catgcgcccg tgccaggcgg
      241 ccgcgccgag caccagctgc cgcgcgatat cgttgtcgtt ggtgatgacg gaaccggcca
      301 ggctgccgtc gccgagtcgc gccagctcga ttgcctcgtc caggttgttg taaggaagca
      361 cggtgctgac gggcccgaaa gcctcgacgg aatgcacggc ggtcgcggaa agagggcgtt
      421 cacagtgcag cagcgtcggc ataaagaatg cccctctgtc cgcatcggca tcgaccaggt
      481 cgaagtcgct gcgattgccg taaacgactt ctgcttcgcc ggagagatct gccacccgcg
      541 cgcgcacttc ctcgcgttgc ccgagactcg cgagcgcgcc catgtcgacc tccttcgagg
      601 cagggttgcc gatgcgaatc tcgccgagcg ccgtcgacaa tgccttcacc aagtcagccg
      661 ccaattccgc aggcgcaatg atccggcgga tggccgtgca tttctggccg accttgctgg
      721 tcatctcgcg cgtcacttct ttaataaaga ggtcgaactc cggcgtgtcg ggcgttgcat
      781 cgggaccgag aatagaagcg ttcagtgaat cggtctccgc cgtgaagtgc accgactcgg
      841 caacgatgcg cggatgctgc tgcagctttt ccgcggtcga tttcgaaccc gtgaatgcga
      901 cgatgtcctg gcaagtcaga tgatcgaaca ggtcgccgac gccgccgcag atcagttgca
      961 gcgatccctc gggcaggatc tgcgactcga tcatgcgccg gaacaccagc tcggtcaggt
     1021 aggcggtgct cgatgccggt ttgacgatcg ccgggacgcc ggcaaggagc gcgggcgcga
     1081 gtttctcgag catcccccag cagggaaaat tgaaggcatt gatgtgcacc gccgcgcccg
     1141 gtcgtggtgt gtagatatgc tggccgacaa aggtgccgtt ccgcgagagc atttccgggg
     1201 cgccgtcgag atagaccttg tcgttgggca tctcgcggcg ccctttgctc gaatacacga
     1261 acagcgtgga gatgccgccg tcgatgtcta tccaggagtc tgctctggtg gctcccgttt
     1321 gcgtggacag ggcgtaaaat tccttcttgt gctccatcag gtacttggcg agttccttca
     1381 acatgagggc gcgctcgtga aaggtgtact tgcgaagatt gctgccgccg ctgcgcctcg
     1441 cgtaatccag catcgcgccg aaatcgagac ccgaactggt gatgctggcc accggctggc
     1501 catccaccgc gctcgtgagg accttgcctt cgtcggcggc agtgaaccag ctgttttctg
     1561 caaaattctg aagattcatc gtgttaatcc cgagcgggcc ggagaggacc cctaagatac
     1621 acaaaatcgg gaaatttgta atttttggtg tcacaatgcc ccggcattgc gcatttgatg
     1681 tgaggcagga tgtcgatcca ggcggcaagc agggcattgc tcgacgaatt tcgttcgcgg
     1741 ccgacgatac gcgcgggctc cctgatcatc accgttttcg gcgacgcggt tgcgccgcgc
     1801 ggcggtaccg tgtggattgg cagcctgatc aaagtgctcg atgacttcgg cgtcagcgaa
     1861 aggctggtca ggacctccgt ttttcgcctg acgcgcgacg cctggctcga tgtcaaccag
     1921 gtcggtcgcc ggagttacta cagcctcacc gcggacggcg cgcggcgctt tgagcaggcg
     1981 acgcaaagaa tctacggtga gccgcggcag agctggtcgg gtgactggtg cctcgttctg
     2041 ctcgcggacc tcgctgcgga acagaaagac atcgtacgcc gggaactcgg ctggcagggc
     2101 tttgccgcga tttccaccaa cgtactcgcg catccgtcgc aaccgaaacc ggaaatcgaa
     2161 cggacgctcg aaggcctcgg tgtcgagcgc gacctggtcg tgatgcaggg ccggaccctc
     2221 ggcaagaacc aggatgaagc gatgcgtacg ctggtgcaca agagttggaa cctcgacgac
     2281 atcga
//
LOCUS       DATCZV010000220         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_69548, whole genome shotgun sequence.
ACCESSION   DATCZV010000220 DATCZV010000000
VERSION     DATCZV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69548"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..234
                     /gene="ccmD"
                     /locus_tag="VIW27_04895"
     CDS             76..234
                     /gene="ccmD"
                     /locus_tag="VIW27_04895"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY5739038.1"
                     /translation="MLEFFQMGGYAAFVWPSYGLAALVLWLNWYLPRRQHAQELRKLK
                     RRLETERR"
     gene            231..665
                     /gene="ccmE"
                     /locus_tag="VIW27_04900"
     CDS             231..665
                     /gene="ccmE"
                     /locus_tag="VIW27_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY5739039.1"
                     /translation="MTPARKKRLALIVLMVAGVSVGVGFALNSLNQNIMFFLTPSEVL
                     AGEAPRDRLFRVGGMVVDGSVARPGDGLTVHFDLTDNERQVTVRYTGILPDLFREGQG
                     IIANGRLDENGEIVAEEVLAKHDENYMPPELAGMHKPEGAEQ"
     gene            662..2635
                     /locus_tag="VIW27_04905"
     CDS             662..2635
                     /locus_tag="VIW27_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY5739040.1"
                     /translation="MIPEIGHFALLIALCIAFIQAGLPLAGASLGIQRWVGVAVPAAL
                     AQLLFLAISYAALTWAFVQSDFSVLYVAQNSNVDLPLIYRISGVWGAHEGSLLLWALV
                     LALWTGAVAVFTPNVPRAMRARVLGVLGLVSIGFLLFIVITSNPFERLLPAATQGRDL
                     NPLLQDIGLAIHPPMLYFGYVGFSVAFAFAIAALISGQVDSAWARWSRPWTNIAWLFL
                     TLGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAITEKRGTFKAWTA
                     LLAIFSFSLSLLGTFLVRSGVLTSVHAFATDPERGIFILGFLVVVVGGSLVLYAWRAP
                     MLKSAAKTNLLSRDGMLLLNNIFLAVATAMILLGTLYPIAIDAMGGKISVGPPYFNLM
                     FISLTAPLAVLMGVGMSIRWKEDTLTRLRSRLWVPALLALAVGLALALVLPEWRWGAF
                     AGLAMAVWITFTAGLAIYERLHGRSLGSALRSTPAGFYGMLLGHLGIAVFIVGVTLTS
                     LYNTEKDLRMEPGDIYSVAGYEFRFNGVREFNGQNYVATRGAFSVRAVDGDFRAEMFP
                     EKRIYRVQTMPMTEAAIDAGFTRDLFIALGEPIGTGGAWAVRVYYKPFIRWIWLGAII
                     MAVGGLFAASDRRYRRLVRSRSETGQAATERSV"
BASE COUNT          413 a          818 c          921 g          581 t
ORIGIN      
        1 ctggcgctgt ttcacaaatg ccgcgccgag ttactggtgc gcgagcgcaa cagtaagtgg
       61 gttgaggaac tttaaatgct ggaatttttc caaatgggtg gttacgccgc gttcgtctgg
      121 ccgagctacg ggctggcggc gctggtgttg tggctgaact ggtatttacc gcgcaggcaa
      181 cacgcgcagg agttacgtaa attgaaacgt cgacttgaaa cggagcgccg atgaccccgg
      241 cgcgcaaaaa aagactggca ctgatcgtgc tgatggtggc cggtgtctcg gtgggcgtgg
      301 gtttcgcgct caattcgctg aaccagaaca tcatgttctt tctcacgccc agcgaagtgc
      361 tggccggcga agcgccgcgc gaccggctgt ttcgcgtcgg cggcatggtc gtcgacggca
      421 gcgttgcgcg gcccggagac ggcctgacgg tgcatttcga cctgaccgac aacgagcgcc
      481 aggtgacggt gcgctatacg ggcattctgc cggacctgtt ccgcgagggg cagggcatca
      541 tcgccaatgg ccggctcgat gaaaacggcg agatcgtcgc cgaggaagtg ctcgccaagc
      601 acgacgaaaa ctacatgccg ccggagctcg ccggcatgca caagccggag ggtgcagaac
      661 aatgattcct gagatcgggc atttcgcgct gctcatcgcg ctgtgtatcg ccttcatcca
      721 ggcgggattg ccgctggccg gtgccagcct cggcattcag cgctgggtcg gcgtcgcggt
      781 gccggccgca ctcgcacaat tgctgttcct ggcgatttcc tacgccgccc tgacctgggc
      841 attcgtgcag agcgacttct cggtgctgta cgtcgcgcag aattcgaatg tcgacctgcc
      901 cctgatatac cgcatttcgg gggtctgggg cgcgcacgag ggctcgctgc tgttgtgggc
      961 gctggtgctg gcgctgtgga ccggcgcggt ggcggtgttc acgccgaatg tgccgcgggc
     1021 gatgcgcgcg cgcgtactcg gcgtgctcgg tctggtcagc atcggctttc tgctgttcat
     1081 cgtcatcacc tcgaacccgt tcgaacgcct gctgccggca gccactcagg ggcgggatct
     1141 gaatccgctg ctgcaggata tcggcctcgc gattcatccg ccgatgctgt acttcggcta
     1201 cgtcggtttc tcggtagcct tcgcctttgc catcgccgcg ctgatttcgg ggcaggtcga
     1261 ctcggcctgg gcgcgctggt cacggccgtg gactaatatc gcctggctgt tcctgacgct
     1321 cggtatcgcg ctcggcagct ggtgggccta ctacgaactt ggctggggcg gctggtggtt
     1381 ctgggacccg gtggaaaacg cgtcgttcat gccctggctg gtcggcactg cgctgatgca
     1441 ctcgctggcg attaccgaga agcgcggtac cttcaaggcg tggacagcgc tgctggcgat
     1501 cttttcgttt tcgctgagcc tgctcggcac ctttctggta cgctccggcg tgctcacctc
     1561 ggtacatgcc ttcgccaccg atcccgagcg cgggatcttc attctcggct tcctggtggt
     1621 ggtcgtcggc ggatcgctgg tgctctacgc ctggcgtgcg ccgatgctga agagtgcggc
     1681 gaaaaccaac cttttgtcac gcgacggcat gttgttgttg aacaacatat tcctcgccgt
     1741 ggccaccgcg atgattctgc tcggcacgct gtacccgatc gcgatcgacg cgatgggcgg
     1801 caagatctcg gtgggtccgc cttatttcaa cctgatgttc atcagcctga ccgcgccgct
     1861 ggcggtattg atgggcgtcg gcatgtcgat ccgctggaaa gaagacacgc tcacgcgctt
     1921 gcgatccagg ctgtgggtgc cggcactgct ggcgctggca gtggggctgg cgctggcgct
     1981 ggtgctgccg gagtggcggt ggggcgcctt tgcgggtctg gcgatggcgg tctggatcac
     2041 ctttaccgcg gggctggcga tttacgagcg gctgcacggc cgttcgctgg gttcggcgct
     2101 gcgttccacg cccgccggct tctacggcat gctgctcggg cacctgggta tcgcggtatt
     2161 cattgtcggc gtgaccctga ccagtctcta caacacggag aaggatctgc gcatggagcc
     2221 cggcgacatc tactcggtgg ctggttacga attcaggttc aacggggtgc gcgaattcaa
     2281 cggccagaac tacgtcgcca cgcggggtgc gttcagcgtg cgcgccgtcg acggcgattt
     2341 ccgggccgag atgttcccgg aaaagcgtat ctaccgggtg cagacgatgc cgatgaccga
     2401 agccgcgatc gacgccggtt ttacccgtga cctgttcatc gcgctcggcg agccgatcgg
     2461 caccgggggc gcctgggcag tacgggtgta ttacaagccg ttcatccgct ggatctggct
     2521 cggcgcgatc atcatggccg tcggcggcct gttcgcggcc agcgaccgcc gctaccggcg
     2581 cctggtcaga agccgtagcg agaccggcca ggcggccaca gaacgtagcg tatgaatcgc
     2641 ttcgttctgc cgctggcggt tttcgtgctg atggtcggtt tgctcggcta tggcctgagc
     2701 ctcgatccga agaaggtgcc gtcgccgctg atc
//
LOCUS       DATCZV010000221         4526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_69606, whole genome shotgun sequence.
ACCESSION   DATCZV010000221 DATCZV010000000
VERSION     DATCZV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69606"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VIW27_04910"
     CDS             complement(<1..481)
                     /locus_tag="VIW27_04910"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEY5739041.1"
                     /translation="MARVQSFEPVIGKNPRAIILGSMPGVASLEAVQYYAHPRNAFWP
                     IIGELFGFDHAVDYETRIDAIRQLPIILWDSLLSCRRPGSLDSNIDIETAQANDFPGL
                     LRKYPQVRAVFFNGAAAEKYFRKLALPKLPEGVDIELQRLPSTSPANAGMSFERKLAA
                     "
     gene            complement(489..1745)
                     /gene="rho"
                     /locus_tag="VIW27_04915"
     CDS             complement(489..1745)
                     /gene="rho"
                     /locus_tag="VIW27_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5739042.1"
                     /translation="MNLNELKQNTVPELIETLKDMGGDHSARMRRQDIIFSILKSQAK
                     KGEDIFSEGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKDNERYFALLKVDQINFDLPENVKHKVLFENLTPLHPNERMKLERGNGSTEDITAR
                     IIDLVSPIGKGQRGLVVSPPKAGKTLMLQNIAQSIASNHPECYLIVLLIDERPEEVTE
                     MERMVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     TIPSSGKVLTGGVDAHALHRPKRFFGAARNIEEGGSLTILATALVETGSKMDEVIYEE
                     FKGTGNMEIHLDRRIAEKRVFPAININRSGTRREELLIDQDELQKVWILRKFLHSMDE
                     IEAMEFVLGRMQNSKTNLEFFDQMKK"
     gene            complement(2100..2426)
                     /gene="trxA"
                     /locus_tag="VIW27_04920"
     CDS             complement(2100..2426)
                     /gene="trxA"
                     /locus_tag="VIW27_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY5739043.1"
                     /translation="MSDKIVYLSDESFESDVLQADGPVLVDYWAEWCGPCKMIAPILE
                     EIAEEYDGRLTVAKLNIDDNANTPPKYGIRGIPTLMLFKGGSVEATKVGALSKSQLTA
                     FLDSNI"
     gene            2647..3990
                     /locus_tag="VIW27_04925"
     CDS             2647..3990
                     /locus_tag="VIW27_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5739044.1"
                     /translation="MAQQHLTEQKFGSLSLDQRLLDALSRLDFQYCTPIQAKALPLLL
                     EGKDISGQAQTGTGKTLAFLLACGQRLLTSPRPPTEPGKPRVLILAPTRELAIQIHKD
                     AERLFQDLPLRLAICYGGKAYEQQKRQFDEPVDILIGTPGRLIDFFKQRLFDFKSIEV
                     MVLDEADRMFDMGFIADVRYMLRRLPPPDQRLNMLWSATLAQKVMELAYEHMNEAQSI
                     TIDADTPAVERIEQSVYHPANHEKIPLLLGLLKKLEPPRAIIFANTKYATIKIWEYLQ
                     GNGLSAAIISGDIHQNKRESLLKRFHDGEYSILVATDVASRGLHIPDVTHVFNYDLPE
                     LGEDYVHRIGRTARAGKSGMAISFACEDHAMNLIDIESYTRRPIPTVSISNELLIKPE
                     PPVKMKGGRLKPGARGGKPHGGQGRGGGRGSGGNQQRPRRRGGSRPQNQRKQQTG"
     gene            3987..>4526
                     /locus_tag="VIW27_04930"
     CDS             3987..>4526
                     /locus_tag="VIW27_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY5739045.1"
                     /translation="MNRKNNVLIIGGSGFIGKHLAVALANRGYQVTVPCRRPHRNRDL
                     LVHPDIRVLQAEVGDPAELQNLCAEQQVVINLIGILHEKRKGDFRRMHVEFVKQVIEA
                     CKQAGIQRLLHVSALGANEASGSSLYLRSKGEGENLLHTLGQRGLRVTSFQPSVVFGK
                     GDQFINQFAGILRWCFGYFP"
BASE COUNT          921 a         1351 c         1331 g          923 t
ORIGIN      
        1 aggcggcgag tttgcgttcg aagctcatgc cggcattggc cgggctggtc gacggcaggc
       61 gctgtaactc gatatccacg ccctccggta atttcggcag tgcgagtttg cgaaagtatt
      121 tttccgctgc cgcgccgttg aaaaagaccg cgcgaacctg cggatacttc cgcaacagcc
      181 ctgggaaatc gttggcctgc gcggtttcga tatcgatatt tgaatccagg ctgccggggc
      241 gacgacaact cagcagcgaa tcccacaaaa tgatcggcag ttggcgaatc gcgtcgatcc
      301 gggtctcata atcgaccgcg tggtcgaagc cgaacagctc gcctatgatc ggccagaagg
      361 cgttgcgcgg atgcgcgtaa tactgcaccg cctcgagcga cgcaacgccc ggcatgctgc
      421 cgaggatgat ggcgcgggga tttttgccga tgaccggttc gaacgactga acccgggcca
      481 cgggccgctt atttcttcat ctggtcgaag aattcgaggt tggtcttgct gttttgcatg
      541 cggccgagca cgaattccat cgcctcgatc tcgtccatcg aatgcaggaa cttgcgcagg
      601 atccagactt tctgcagttc gtcctggtcg atcagcaatt cctcgcggcg ggtaccggaa
      661 cggttgatat tgatcgccgg gaatacgcgc ttctcggcga tgcgccggtc gagatggatt
      721 tccatgttgc cggtgccctt gaattcctcg tagatgactt cgtccatctt cgaaccggtc
      781 tccaccagcg cggtcgccag aatcgtcagg ctgccgcctt cctcgatgtt gcgcgcggcg
      841 ccgaagaaac gcttcggccg gtgcagcgca tgcgcgtcga caccgccggt caacaccttg
      901 cccgaagacg ggatcgtggt gttgtaagcg cgcgcgagac gcgtgatcga gtcgagcaga
      961 atgaccacgt cgcgcttgtg ctcgaccagc cgcttggctt tttcgataac catttcggcg
     1021 acctgcacat ggcggctggc aggctcgtcg aaggtcgacg cgaccacctc gcccttgacc
     1081 atgcgctcca tctcggtaac ttcttccggg cgctcgtcga tcagcagcac gatcaggtag
     1141 cattccggat ggttgctggc gatgctctgc gcgatgttct gcagcatcag tgtcttgccg
     1201 gccttcggcg gcgataccac gaggccgcgc tgccccttgc cgatcggcga caccaggtcg
     1261 atgatgcgcg cggtgatatc ctcggtactg ccgttgccgc gctcgagttt catgcgctca
     1321 ttggggtgca gcggcgtcag gttctcgaac aggaccttgt gcttgacgtt ctccggcagg
     1381 tcgaaattga tctggtcgac cttgagcagc gcgaagtaac gttcgttgtc cttcggcggc
     1441 cttatcttgc cgctgatggt atcgccggta cgcaggttga agcgacgaat ctggctcggc
     1501 gacacgtaga tgtcatccgg cccggcgaga taggaactgt ccgcggagcg caggaagccg
     1561 aagccgtcct gcaggatttc gagcactccc tcgctgaaga tatcctcgcc ctttttcgcc
     1621 tgggacttga gaatgctgaa aataatgtcc tggcgtcgca tgcgggccga gtgatcgccc
     1681 cccatgtcct tcagggtttc gatgagttcg gggacggtgt tttgcttcag ctcgtttaga
     1741 ttcatattag ggagtgcact acatgcaggg ttgggtcagt caaagacgta agaacggcct
     1801 atgggtggaa cgggaactga ttcggttttt tattacggga tttgccgacg gggacgcggc
     1861 aacgaataac gaagcctagc acatgaacca ggggtcgtcc accaaataca gccgcctttt
     1921 tgtcaattaa atctgccgct tcgatcgccc tcggcggcaa ccagggtact cttggagagc
     1981 cttgcgcggc gcggcttgca gcttgccgcc gccgaacgct ccggggtttc gccggacacc
     2041 gttatcgagc cggtgtcagc gaatagtctc gcactgccgg cgagacccgc agctgagtgc
     2101 tagatattgc tgtcgagaaa ggcagtcaac tgggatttcg acagtgcacc gaccttggtt
     2161 gcctcgactg acccgccttt gaacagcatc aatgtcggga tgccgcgaat accgtatttc
     2221 ggtggcgtat tggcgttgtc gtcgatgttg agcttggcga cggtgaggcg tccatcgtac
     2281 tcttcggcga tttcttcgag tatcggcgca atcatcttgc agggaccgca ccactcggcc
     2341 cagtaatcga ccagcaccgg cccatcggcc tgcaatacgt cagattcaaa gctctcgtcc
     2401 gaaaggtata ctattttatc actcacacgt ttctccagcc cgcaggcttt ttcagcagga
     2461 ccgacaattt agtcgcatca gttttaactg tcaataatgc cgccggcgag cgattgtcgc
     2521 gaacctggca cccccattcg cgagcatata ggcacactgt ccggtttttc aaatcacagc
     2581 gcaggttttt ttccaatttg ctaccatagg ccgattaacc aacaacctgc gagaatcagc
     2641 gacagtatgg cgcaacaaca tttaaccgag cagaaattcg gctctctatc actggatcag
     2701 cgcctgctgg acgccctgtc ccgactcgac ttccagtatt gcacgccgat ccaggccaaa
     2761 gcgctgccgt tgctgctcga aggcaaggac atttccggcc aggcgcaaac cggaaccggt
     2821 aagacgctgg cgttcctgct cgcctgcggg cagcgcctgc tgacctcgcc gcggccgccg
     2881 accgagccgg gcaagccgcg cgtactgata ctcgcgccga cccgcgagct ggcgatccag
     2941 atccacaagg acgccgaacg gctgttccag gacctgccgt taaggctggc gatctgttac
     3001 ggcggcaagg cttacgaaca gcagaagcgg caattcgacg aacccgtgga catcctgatc
     3061 ggaacgccgg gacggctgat cgactttttc aagcagcggc tgttcgattt caagtcgatc
     3121 gaagtcatgg tgctcgacga agccgaccgc atgttcgata tgggcttcat cgccgatgtg
     3181 cgctacatgc tgcggcgcct tccgcccccc gaccagcgcc tcaatatgct ctggtccgca
     3241 accttggcgc aaaaggtcat ggaactggcc tacgagcaca tgaacgaagc gcaatcgatc
     3301 accatcgacg ccgatacgcc agcggtggaa cgtatcgagc aaagcgtcta tcacccggcc
     3361 aaccacgaaa agatcccgct gttgctcggc ctgttgaaga aactcgagcc gccgcgcgcg
     3421 ataatcttcg ccaataccaa gtacgcgacc atcaaaatct gggaatacct gcagggcaac
     3481 gggctgtcgg cagcgatcat ttccggcgac attcaccaga acaagcgcga aagtctgttg
     3541 aaaaggtttc acgacgggga gtactctatc ctggtcgcga ccgacgtcgc gtcgcgcgga
     3601 ttgcacattc cagatgtcac gcatgtcttc aattacgact tgcccgaact cggcgaagac
     3661 tacgtgcacc ggatcggcag aaccgcgcgg gccggcaagt cggggatggc gataagcttt
     3721 gcctgcgagg atcacgcgat gaatctgatc gatatagaat cctacacccg caggccaatt
     3781 ccgaccgtat cgatcagcaa tgagttgttg atcaagcccg aaccgccggt gaagatgaaa
     3841 ggcggacggc tgaaacctgg cgcccgaggc ggcaagccgc acggcggcca gggacgcgga
     3901 ggcggccgcg gaagcggtgg caaccagcaa cggccgcgcc gccggggcgg ttcgcggcct
     3961 caaaaccagc ggaaacagca gacaggatga accgaaaaaa caacgttcta atcattggcg
     4021 gcagcggctt tatcggcaag cacctggcgg ttgcgctggc caatcgcggc taccaggtca
     4081 cggtgccgtg ccgcaggccg caccgcaacc gcgacctgct ggtccatccc gacattcgcg
     4141 tgctgcaggc cgaggtcggc gatccagccg aactgcaaaa cctgtgcgcg gaacagcagg
     4201 tcgtcatcaa cctgatcggc atcctgcacg aaaaacgcaa gggcgatttt cgccgcatgc
     4261 atgtcgagtt cgtcaagcag gtcatcgagg cctgcaagca ggccggcatt caacgcctgt
     4321 tgcacgtcag tgcgctcggc gccaacgagg cctcgggcag cagtctctac ctgcgcagca
     4381 agggcgaagg cgaaaacctg ttgcataccc ttggccagcg cggcctgcgg gtaaccagtt
     4441 ttcagccgtc ggtggtgttc ggcaagggag accagttcat caatcagttc gccggcattt
     4501 tgcgctggtg cttcggctat tttccg
//
LOCUS       DATCZV010000222         6019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_547353, whole genome shotgun sequence.
ACCESSION   DATCZV010000222 DATCZV010000000
VERSION     DATCZV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547353"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VIW27_04935"
     CDS             complement(<1..191)
                     /locus_tag="VIW27_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEY5739046.1"
                     /translation="MKLTDFKMLSFDTYGTLIDWESGIYAALQPLLDKVPIKLERNEV
                     LELFAEYELAQQRATPTQV"
     gene            319..807
                     /locus_tag="VIW27_04940"
     CDS             319..807
                     /locus_tag="VIW27_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5739047.1"
                     /translation="MSDTLPAIREFLEHCGYAHEVWPCDPELADTAVFCEHYGVALEN
                     SANAILVRSKTGTRKFVVCVLLATDRLNTNHTVRKRMGARKVSFASADETREMTGMEI
                     GGVTPLALPAGLPIWIDAAVMECEYVVLGGGNRESKIRLDPRALLEQATAEVVAGLAL
                     RK"
     gene            1001..2281
                     /locus_tag="VIW27_04945"
     CDS             1001..2281
                     /locus_tag="VIW27_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739048.1"
                     /translation="MYKLIKVESREIPSAAVSFLFAFTLMASYYLLRPVRDAMSSDWS
                     DAELSWLWTLNFFISTAAVLVYGWVVSRVPLKRLVPGIYLFFALSFVLFYAAAGWLPD
                     TGLIDKAFYVWVSFFALFHISVFWSFTSDIYTRAQAKRLFGLFGAGASIGAIAGPALT
                     VSLVDSVGVYNLLLIAAMMLVVLLPMIFFLERSRVATAGQGQPRSDPLASIGGNLLGG
                     FQDFISHRFLLGIGLFILLYTLMSTFVYFELKNAMVDYDRAARSQYWGIMDLAVNTLA
                     ILTALFATGRLATRWGLAVTLALVPVIMVFGWLAVAVAPGLMALIGLQIVRRAGNYAI
                     TKPGREMLFTGVERDTRYKTKPVIDIVVYRGGDVAAGWTYTALAQGVGLGLGAIALAA
                     AAIAVGWALVGVFLGNRHERAAGAEPKPALLTET"
     gene            2283..3221
                     /locus_tag="VIW27_04950"
     CDS             2283..3221
                     /locus_tag="VIW27_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5739049.1"
                     /translation="MKDATRGAFTRRRFIKSTAAAALLGPLLPAAGQATQSGAIKKTI
                     PSSGERLSVIGLGTSRTFNVAPDDAAQSPLVDVMQAFFDRGGQLIDSSPMYGNAEAVT
                     GVLLDQVANKQELFTATKVWTNGKRAGIEQMQASMRHFGVERIDLMQIHNLRDWRTHI
                     ETLKEWKADGRIRYIGITTSHGRYHAELEQLLQELPLDFVQFSYNIANRDVEQDLLPL
                     AAERGIATLINRPYQRGSLFRAVRGKPLPDWAAEIDCASWGQYFLKFIAAHPGVTCVI
                     PATSKVKHMVDNMGAGFGRLPDAAMRKRMIAYLESI"
     gene            complement(3296..4324)
                     /locus_tag="VIW27_04955"
     CDS             complement(3296..4324)
                     /locus_tag="VIW27_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5739050.1"
                     /translation="MKNTAENPVDVLILALPETAGSALYGMLDVLSATGNLWQTLVRS
                     GPQSRCFRVRILGVQEAPFTCGNGIPVHPQVSIADDPLASILILPEIWLGPDESIHGR
                     YPALIDWIRRQHRHGAEIYSACSGSILLAETGLLDGRAATSHWGYADLFRRQYSKVRF
                     DAASNLVYADPGARIVTAGGTTSWHDLALHIIARHAGPGAALQIAKVYLLKWHPEGQL
                     PYTGLVRRAPHADSVARRAEDWLAENYREIDAIRRVVELAGIPERSLKRRFKMATGST
                     LIEYLQNLRIEQAKLLLEQGELPIEAVSEAVGYMDVSFFRRLFKRLTGLPPGQYRRMF
                     QSQARLTA"
     gene            4470..5057
                     /locus_tag="VIW27_04960"
     CDS             4470..5057
                     /locus_tag="VIW27_04960"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5739051.1"
                     /translation="MSQQNSQQSAVESREAVFVNGVDLDALGETVSAIQADPELGVSR
                     FRAANRWAGGNRNESTVTGFYGAKQEIAHRQAFTLIADEPPILAGNDDGANPVEHLLH
                     ALASCLTTSMVAHAAVRGIKIEELEARLEGDIDLNGFLGLNPNTPKGFTAIRAEYRVN
                     AAPEDLEKIASLASFSPVFNTLTNGVPVEVRVEAR"
     gene            5157..5567
                     /locus_tag="VIW27_04965"
     CDS             5157..5567
                     /locus_tag="VIW27_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739052.1"
                     /translation="MQQKTHDGRRGGGIKTKFAACLEAPWPSATRILPTRQRHAALAR
                     LESRSLEDAGITTEPSSTSCQTLAEELEMITAACDSSVREDDRAGTAAQTSVQSPWSA
                     DTSVARDSLAANCNREIDAWARHALAANGFGDLA"
     gene            5823..>6019
                     /locus_tag="VIW27_04970"
     CDS             5823..>6019
                     /locus_tag="VIW27_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014593234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5739053.1"
                     /translation="MSFESLGLRAELLRAVVEKGYATPTPIQTQAIPLILDGRDLMGC
                     AQTGTGKTAGFTLPILQRLAAS"
BASE COUNT         1126 a         1962 c         1846 g         1085 t
ORIGIN      
        1 tagacctgcg tcggcgtggc gcgctgttgc gcgagctcgt actcggcgaa caattcgagc
       61 acctcgttgc gctcgagctt gatcggtacc ttgtcgagca ggggttgcag cgcggcgtag
      121 atgccgctct cccagtcgat cagcgtaccg taggtatcaa agctcagcat cttgaagtcg
      181 gtcagtttca ttttcggctg cctggtgcgt cggtcgaggc cccaattctg accgacgcga
      241 cgattaaatc aagacgctgt tttgcgccgc caccgaaacc tgttgtataa agtcgggcaa
      301 accgacggcc tggcaatcat gagcgatacc ctgcccgcga tacgcgaatt tctcgaacac
      361 tgcggctatg cgcacgaagt ctggccctgc gacccggaac tcgccgacac cgcggtattt
      421 tgcgaacact acggcgtggc gctggagaac tcggccaatg cgatcctggt gcgttcgaaa
      481 accggcacgc gcaagttcgt ggtctgcgtg ctgctcgcca ccgatcggct caacaccaat
      541 cacacggtgc gcaagcgcat gggtgcgcgc aaggtgtcgt tcgcgtcggc cgacgaaacc
      601 cgcgaaatga ccggcatgga gatcggcggc gtgacgccgc tggcgctgcc cgcggggttg
      661 ccgatctgga tcgacgccgc ggtaatggaa tgcgaatacg tggtgctcgg cggtggcaac
      721 cgcgagtcga agatcaggct cgatccgcgc gcattgctcg agcaggcgac cgccgaggtc
      781 gtcgcaggcc tggcgttgcg caaataggac agcccgggca aacggcattc ggggtcggcc
      841 ccgctccgcc ggtcgagtcg cggattcccg cttgcgggcg gcgaaatccg gcaaacaaca
      901 aacggaccgc gcgagcggtc aacctcgatc ccggcggatt cgagtataat gccgggcata
      961 gccgttgcat cgaactgtta ccgtaagctt gccaaacctg ttgtacaaac tgatcaaggt
     1021 cgagtcgcgc gaaattccgt cggcggcggt atccttcctg ttcgcattca cgctgatggc
     1081 gagctattac ctgctgcgac cagtgcgcga cgcgatgtcg agcgactgga gcgatgccga
     1141 attgtcgtgg ctgtggacac tgaacttctt catcagcacc gccgcggtgc tggtttacgg
     1201 ctgggtggtt tcgcgcgttc ccttaaagcg gctggtgcct ggaatctacc tgttcttcgc
     1261 actctccttt gtgctgtttt acgcggccgc tggctggctg ccggacaccg gcctcatcga
     1321 caaggcgttc tacgtctggg tcagtttttt cgcgctgttc catatctcgg tgttctggag
     1381 tttcacctcc gatatttata cccgcgcgca agcaaaacgc ctgttcggcc tgttcggtgc
     1441 cggcgccagc atcggtgcca tcgccggccc ggcgctgacc gtgtcgctgg ttgattccgt
     1501 cggcgtctac aacctgttgc tgatcgcggc aatgatgctg gtagtgctgt tgccgatgat
     1561 ctttttcctc gaacgcagcc gcgtcgccac cgcgggccag ggtcagccgc gtagcgatcc
     1621 gctggcctcg atcggcggca acctgctggg cggtttccag gatttcatca gccaccgttt
     1681 cctgctcggg atcggcctgt tcatcctgtt gtacacgctg atgagcacct tcgtttactt
     1741 cgagctgaag aacgcgatgg tcgactacga tcgcgccgca cgctcgcagt actggggcat
     1801 catggacctg gcggtcaaca cgctggcgat cctgaccgcg ctgtttgcca ccggccgcct
     1861 ggcgacccgc tgggggctcg cggtgacgct ggcgctggtg ccggtgatca tggtcttcgg
     1921 ctggctcgca gtggccgtgg cacccggcct gatggcgctg atcggcctgc agatcgtgcg
     1981 ccgcgccggc aattacgcga tcaccaagcc gggccgcgaa atgctgttta ccggcgtcga
     2041 acgcgatacc cgttacaaga ccaaaccggt gatcgacatc gtcgtctatc gcggcggcga
     2101 cgttgccgct ggctggactt acaccgcgct ggcccagggc gttggcctcg ggctcggcgc
     2161 gatcgccctg gcggccgcgg caatcgcggt cggctgggcg ctggtaggag tatttctcgg
     2221 caatcgtcac gaacgcgccg ctggcgccga accgaaaccc gccctgctca cggagacctg
     2281 aaatgaaaga tgcaacccgt ggcgccttta cgcggcgccg gtttatcaaa tccaccgcgg
     2341 ccgccgcgct actcgggccg ctgctgcccg ccgccggcca ggcgacgcaa tccggcgcga
     2401 tcaagaagac gataccctcc agcggcgagc gcctgtcggt gatcgggctc ggcacttcgc
     2461 gcaccttcaa cgtcgccccc gacgatgccg cgcaatcgcc gctggtcgac gtcatgcagg
     2521 cgtttttcga ccgtggcggg cagctgatcg attcatcacc gatgtatggc aacgccgagg
     2581 cggtcaccgg ggtgctgctc gaccaggtcg ccaacaagca ggagctgttc accgcgacca
     2641 aggtatggac caacggcaag cgcgccggca tcgagcagat gcaggcttcg atgcgccact
     2701 tcggcgtcga gcgcatcgac ctgatgcaaa tccacaacct gcgcgactgg cgcacgcaca
     2761 tcgaaaccct caaggaatgg aaggccgacg gccgaatccg ctatatcggc atcaccacgt
     2821 cccatgggcg ctaccatgcc gaactcgaac agctgttgca ggaactgccg ctcgatttcg
     2881 tgcagttcag ctacaacatc gccaaccgcg acgtcgagca ggacctgttg ccgttggccg
     2941 ccgagcgcgg catcgcgacg ctgatcaacc gtccctacca gcgcggcagc ctgtttcgcg
     3001 cggtgcgcgg caagccgctg cccgactggg cggcggaaat cgactgcgcg agctgggggc
     3061 agtattttct caagttcatc gccgcgcatc ccggcgtgac ctgcgtcatt ccggcgacct
     3121 ccaaggtcaa acacatggtc gacaacatgg gcgccggctt cggccgcctg cccgacgcgg
     3181 ccatgcgcaa gcgtatgatc gcctacctgg agtccatctg acgctcggcg acgcggcccc
     3241 gaggagaatt tcagatctga cccaaattcg gtgaccgcgg gatcgcggcg ccgaatcagg
     3301 ccgtgagccg agcctgggac tgaaacatgc gccgatactg gcccggcggc aaaccggtga
     3361 ggcgcttgaa caggcgcctg aaaaacgaga catccatgta gccgaccgcc tcgctgaccg
     3421 cctcgatcgg cagttcgccc tgctccagca atagcttggc ctgctcgatc ctcaggtttt
     3481 gcagatactc gatcagcgtg ctgccggtgg ccatcttgaa gcgccgcttc agcgaccgtt
     3541 cgggtattcc ggccagctcg acgacgcgcc tgatggcatc gatctcgcgg taattttcgg
     3601 ccagccagtc ctcggcccgc cgcgccaccg aatcggcgtg cggcgcgcgg cgtacgaggc
     3661 cggtataggg taactgccct tccgggtgcc atttcagcag atagaccttg gcgatctgca
     3721 gcgcggcgcc aggacccgcg tggcgagcga tgatatgcag cgcgagatcg tgccacgagg
     3781 tcgtgccgcc ggcggtcacg atgcgtgcgc cggggtcggc gtaaaccagg ttcgacgcgg
     3841 catcgaagcg caccttcgaa tactggcggc gaaacaaatc cgcgtagccc cagtgcgagg
     3901 tcgccgcgcg accgtcgagc aggccggttt cggcgagcag gatcgagccc gaacaggccg
     3961 agtagatttc ggcgccgtgg cgatgctgac gtcgaatcca gtcgatcagc gccgggtaac
     4021 gcccgtgaat cgattcgtcc ggcccgagcc agatctcagg caggatcagg atcgaggcca
     4081 gcgggtcgtc ggctatcgaa acctgcgggt gcaccggaat accgttgccg caggtaaagg
     4141 gcgcctcctg gacacccagg atgcgcactc taaagcagcg cgactgcggc cccgaacgca
     4201 ccagcgtctg ccacaggttg ccggtcgccg acaacacgtc gagcattccg taaagcgccg
     4261 acccggccgt ctccggcaaa gccaatatca gcacatcgac tggattttca gcagtatttt
     4321 tcataaatac gtaaatttta tggcataaat agcctgtttt tgtattttat gcatctaacg
     4381 ctcacgctgt caatcgctca tagtttcacc tgccgaggga gctttcccga cgccaattcc
     4441 aactttcagc gaacccagga gcaaaaaaca tgagccaaca aaatagccaa cagtcggccg
     4501 tcgagtcccg cgaggcggtt ttcgtaaacg gcgtcgatct cgacgcactc ggcgagaccg
     4561 tcagcgcgat ccaggccgat cccgaactcg gcgtgagccg cttccgcgcc gccaaccgct
     4621 gggcgggcgg caaccgcaac gagagcaccg tgaccggctt ttacggcgcg aaacaggaaa
     4681 tcgcacaccg gcaggcgttc acgctgatcg ccgacgagcc gccgattctc gccggcaacg
     4741 acgacggcgc caacccggtc gagcacctgc tgcacgcgct ggcctcttgc ctgacgactt
     4801 cgatggtggc ccacgcggcg gtgcgcggca tcaagatcga ggaactggaa gcccgcctcg
     4861 aaggcgatat cgatctcaac ggctttctcg gcctgaatcc gaacaccccg aaggggttta
     4921 ccgcgattcg cgccgagtac cgtgtcaacg cggcccccga agatctcgag aaaatcgcct
     4981 cgctggcgag cttttcgccg gttttcaaca ccctgaccaa cggcgtaccg gtcgaggtcc
     5041 gcgtcgaggc aaggtaggcg aatcacgcaa tcaccggggt caaccagcgt cggcgctgca
     5101 cccgcccggc gaccccggga ttgcgatccc accaatcccc gaacaggagc acagcaatgc
     5161 aacagaaaac tcacgacggc cgccgcggag gcgggataaa aacgaagttc gcggcgtgcc
     5221 tcgaagcccc ctggccgagc gcgacacgaa tcctgcctac ccggcaacgg cacgcggcac
     5281 ttgcgcggct cgaatcccgc tcgctcgagg atgccgggat taccacagag ccttcatcga
     5341 caagctgcca aacccttgcg gaggagctgg agatgattac tgcagcctgc gattcgagcg
     5401 tccgcgaaga cgaccgggcg ggcacggcgg cgcaaacgtc ggttcagtct ccgtggtcgg
     5461 ccgacaccag cgtagcgcgc gactccctgg ccgcgaattg caatcgcgaa atcgacgcct
     5521 gggcgcggca cgcgctcgcc gccaacggct tcggcgacct cgcctgagcg gatgccgcgg
     5581 tgcacggcgg cacttgttgc cggtggtcac gcggccgcgg cgggcggggc aaaaataaat
     5641 cacctatttg cgcaccattc gtgtatcctg agcccacttt tcggcgccac atcggtcggc
     5701 gccgccattt tcaggtcgat ccgttccgat ttccctggct aacacacgaa tatcaaccct
     5761 gaccgttgac atgcatgcgc gagcagcatt ttcgagggtt gatttccgga gaacaaaaaa
     5821 acatgtcatt cgaatccctt ggcctgaggg ccgagctgct gcgcgccgtt gtcgaaaaag
     5881 gctatgccac cccgacaccg atccagacgc aggcaatccc gttgatcctc gacggccgcg
     5941 acctgatggg ctgcgcgcag accgggaccg gcaaaaccgc cggcttcacg ttaccgatcc
     6001 tgcagcgcct ggctgcctc
//
LOCUS       DATCZV010000223         3814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_441281, whole genome shotgun sequence.
ACCESSION   DATCZV010000223 DATCZV010000000
VERSION     DATCZV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441281"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VIW27_04975"
     CDS             complement(<1..216)
                     /locus_tag="VIW27_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739054.1"
                     /translation="MSAARLNVISHFDKSPWFRCTANPKAVYTQYAGRLVLHDIGNDV
                     VDEYWTLRRSVGLFDVPEKPLEIRGRQA"
     gene            complement(311..952)
                     /locus_tag="VIW27_04980"
     CDS             complement(311..952)
                     /locus_tag="VIW27_04980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5739055.1"
                     /translation="MSDDKISDNQAIDAALRLDGDPERVNAYYEEWARNYNRDVASAG
                     YSGPAISARLLQRHRPDAGIEIIDAGCGTGLAGLALQALGYRRVDGFDLSASMAAEAR
                     TIGCYREVRGEIDIMQASQYYAGVSYDALLSVGVFTLGHVPPEALLELLHLVRNGGVA
                     VLSTRTHYYQQTGFQRLIDELLDAGRIEVLQLLENAAYNHDGDGHYWVLQKRG"
     gene            1072..3114
                     /locus_tag="VIW27_04985"
     CDS             1072..3114
                     /locus_tag="VIW27_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEY5739056.1"
                     /translation="MPADDPLLQPFRLKHLTLKNRLMTTAHEPAYTEDGLPKQRYRLY
                     HRARAEAGLAMTMTAGSALVSRDSPPAFGNILAYREEVVPWLRELVDDCHQFDCRVMI
                     QLTHLGRRAHWNHSDWLPTLSPSGMREAAHRAFPKIIEDWDIERIVADYALAAEHMHA
                     AGLDGIELQCYGHLIDQFWSPVTNFRDDDWGGSLDNRLRFTHLVLDAIRDRVPAEFIV
                     GLRLVVDEQMQSGITREVGLEIARRMVATGQVDFLNVIRGHIDTDAALTDVIPVQGMA
                     SSPHLDFAGEVRAETRFPVFHAARIADIASARHAIASGKLDMVGMTRAHIADPLIVSK
                     LAAGREADIRPCVGATYCLDRIYLAGEALCIHNPASGREATMPHEVATQSERPGKVVI
                     VGAGPAGLEAARVAAARGHRVIVFEATPRAGGQVLLLTRSPRRREMIGIVDWRVEQCD
                     RAGVEFRFNTYAEADEILAAEPDVVVIATGGMADTEVLASGNELAVAARDIIAGDVAP
                     GKRVLLFDDGADHPGLQAAEFIADSGASLEYVSPERQIAPDVGGTNLTPYMRHLLPLD
                     VTFTLCRRALSAQRDGDGIRVTLGSDYGDFTLERHVDQLVVEHGTRPLDAVYFALKPL
                     SVNLGTVDYDALLGGRPQQIERNADGRFRLFRIGDAVASRNIHAAVYDALRLMTTI"
     gene            complement(3124..3552)
                     /locus_tag="VIW27_04990"
     CDS             complement(3124..3552)
                     /locus_tag="VIW27_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739057.1"
                     /translation="MNAMMRNALLVSLLLLAAACSSKSSQPSEPPIVKFGVIAAKQDA
                     TSDQVQSATRGTTSVYGSISTGGRVSIGVGVGVLLGTFGSSSSAEQPVRYDIDLQDGG
                     QITIYHQSPDFAVDDCVRITVYPDEQKHPPDMERSKEGCE"
BASE COUNT          675 a         1298 c         1194 g          647 t
ORIGIN      
        1 cgcctggcga ccgcggattt cgagcggctt ttccggcacg tcgaacaggc ccactgacct
       61 gcgcagcgtc cagtactcgt cgacgacatc gttaccgatg tcgtgcagta ccaggcgccc
      121 ggcatactgc gtgtatacgg ctttcgggtt cgcggtacag cgaaaccacg gagacttgtc
      181 aaagtggctg atgacgttca ggcgcgctgc agacatgcgg gcggctccag ggtcgtggat
      241 ctcggagcgc ctattaaacg caaacccgcc accgctttca atgaaaaccg cgacgcccgc
      301 ggcaattcgg ctagccgcgc ttctgcaaca cccagtagtg accgtcgcca tcgtggttgt
      361 aggcggcgtt ctcgagcagc tgcaaaacct cgatacggcc ggcgtcgagc aactcgtcga
      421 tcagccgctg aaacccggtt tgctggtaat agtgagtacg tgtcgacagt actgcgacgc
      481 cgccgttgcg caccaggtgc agcagttcca gcagcgcctc gggcggtacg tggccgagcg
      541 tgaagacacc gaccgacagc agcgcatcgt agctgacacc ggcatagtat tgcgacgcct
      601 gcatgatgtc gatctcaccg cgcacttcgc gatagcaacc gatcgtgcgc gcctcggcgg
      661 ccatcgatgc cgacaggtcg aaaccatcga cccgccggta cccgagcgcc tgcaacgcaa
      721 gcccggcaag cccggtgccg caaccggcat cgatgatctc gataccggca tcgggccgat
      781 gccgctgcag caggcgcgcc gagatcgccg ggccgctgta gccggcactg gcgacatcgc
      841 ggttgtagtt tcgcgcccac tcctcgtagt aagcattgac gcgttcgggg tcgccgtcga
      901 gacgcagcgc cgcgtcgatg gcctggttgt cggatatctt gtcgtcgctc atgcgcccag
      961 cttaacacga ccgacgagcg ctgccggcgc tgccgcgacg caaccgggtt tgtcgcggcc
     1021 ggttcgcggc tgctaatatt acccgaatct cgaatcgacc ggctaccgcc catgcctgct
     1081 gacgaccccc tgctgcaacc gtttcggctc aagcacctga cgctcaagaa ccggctgatg
     1141 acgaccgccc acgaaccggc ctacaccgag gacggcctgc ccaagcaacg ctaccgactc
     1201 taccaccgcg cgcgcgccga ggccgggctg gcgatgacga tgaccgccgg ctcggcactg
     1261 gtgtcgcgcg acagcccgcc ggccttcggc aacatcctcg cctaccggga agaagtcgtg
     1321 ccgtggctgc gcgaactggt cgacgactgc caccagttcg actgcagggt aatgattcag
     1381 ctgacccatc tcggtcgccg tgcgcactgg aaccattccg actggctgcc gacgctgtca
     1441 ccttccggca tgcgcgaggc ggcacaccgc gcgttcccga agatcatcga ggactgggac
     1501 atcgagcgca tcgtcgccga ttacgcgctc gccgccgagc acatgcatgc ggccggcctc
     1561 gacggcatcg agctgcagtg ctacggccac ctgatcgacc agttctggtc gccggtcacc
     1621 aatttccgcg acgacgactg gggcggctcg ctggacaacc ggctgcgctt cacccacctg
     1681 gtcctcgacg cgatccgcga ccgcgtaccc gcggaattca tcgtcggcct caggctggtg
     1741 gtcgacgaac agatgcaaag cggtatcacc cgcgaggtcg gcctggagat cgcgcgccgc
     1801 atggtggcca ccgggcaggt cgattttctg aacgtgattc gcggccacat cgataccgac
     1861 gccgcgctca ccgacgtgat cccggtgcag ggcatggcgt cgtcaccgca cctggatttc
     1921 gccggcgagg tgcgcgcgga gacccggttc ccggtatttc acgccgccag gatcgccgac
     1981 atcgccagcg cccgccatgc gatcgccagc ggcaagctcg acatggtcgg catgacacgc
     2041 gcgcacatcg ccgatccgct aatcgtcagc aaactcgccg ctggccgcga ggcggatatt
     2101 cggccctgcg tcggcgccac ctattgcctc gatcgcatct atcttgccgg cgaggcgctg
     2161 tgcattcaca acccggccag cggccgcgag gcgacgatgc ctcacgaagt cgctacgcaa
     2221 agcgaacggc caggcaaagt ggtaattgtc ggcgccgggc cggcgggact cgaggcggcc
     2281 cgggtagccg ccgcgcgcgg tcaccgcgtg atcgtcttcg aagccacgcc gcgggccggc
     2341 ggccaggtat tgttgctgac gcgctcgccg cgacgccgcg aaatgatcgg cattgtcgac
     2401 tggcgggtcg aacaatgtga ccgggccgga gtcgaattcc gcttcaatac ctacgccgag
     2461 gccgatgaga ttctcgccgc agagccggac gtggtggtca tcgccaccgg cggcatggcg
     2521 gataccgagg tgctggctag cggcaacgaa ctcgcggtcg ccgcgcgcga catcatcgcc
     2581 ggcgacgtcg cgcccggcaa acgcgtgctg ctgttcgacg acggcgccga tcatccggga
     2641 ctgcaggcgg ccgaattcat cgccgacagc ggcgccagcc tcgagtacgt atcccccgaa
     2701 cggcaaatcg cccccgatgt cggcggcacc aacctgacac cgtacatgcg ccacctgttg
     2761 ccgctcgacg tgacctttac gctgtgccgg cgggcgctgt cggcgcaacg cgacggcgat
     2821 ggcatccgcg tcacgctggg cagcgactac ggcgatttca cactcgagcg ccacgtcgat
     2881 caactggtgg tcgagcacgg cacgcggccg ctggacgcag tctatttcgc gctcaagccg
     2941 ctgtcggtca atctcggcac ggtcgattac gacgcgctgc tcggcgggcg gccacagcag
     3001 atcgagcgca atgcagacgg tagattcaga ttgttccgta tcggcgacgc ggtcgcgtcg
     3061 cgcaatattc acgccgcggt ttacgacgcg ctgcgcctga tgacgacgat ctagaagccc
     3121 cgcctattca cagccttcct tgctgcgctc catatccggc gggtgctttt gctcgtccgg
     3181 gtagacggtg atgcgcacgc agtcgtcgac cgcgaaatcc gggctctggt ggtaaatggt
     3241 gatctgcccg ccatcctgca ggtcgatatc gtagcgcacc ggttgctcgg cggaactgct
     3301 cgagccgaag gtgccgagca acacgccgac gccgacgccg atcgaaaccc tgccgccagt
     3361 cgagatcgaa ccatagacgc tggtggtacc gcgggttgcc gactgcacct ggtcactggt
     3421 ggcgtcctgc ttggcggcga taacgccgaa cttgacgatc ggcggttcgc tcggctgact
     3481 gcttttcgaa ctgcaggcag ccgccagcag cagcaggcta accagtaacg cgttgcgcat
     3541 catggcgttc atcgcttgtg ggttgctacc agtccggtta gagcgcgcaa acagcgcgcg
     3601 agttcccgcc gatagccggc cacgaggccg gccagcggcg ttcgcaccgc gcttattccg
     3661 cgttggcgaa ctcgatgccc ttgcgaatat tgccttcgag tacctcgcgc atctcgtcga
     3721 acagctgctg ctcgtactcg ctgagcggcg gcaaatccgg aatcgacgcg acgccatcct
     3781 tgccgagcac taccggctgg gcatgaaaac tcga
//
LOCUS       DATCZV010000224         4372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_758891, whole genome shotgun sequence.
ACCESSION   DATCZV010000224 DATCZV010000000
VERSION     DATCZV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758891"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VIW27_04995"
     CDS             complement(<1..728)
                     /locus_tag="VIW27_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739058.1"
                     /translation="MLEINGLAIDFLLHGNPVPAVTNASFSIAPGESVALVGESGSGK
                     SVTAQAIMGILPANAVIRGGEIRFDNPETGELVDIARLDPESAQMRRIRGGNISIIFQ
                     EPMTSLSPVHTIGDQISEALFLHRDASRAEGMHLTEAMLQHVGFPDARRALRMYPFEL
                     SGGLRQRAMIAMAMICHPCLMIADEPTTALDVTIQARILKLIRELQAELGMALLLITH
                     DLGVVANMSDQVVVMYHGRVVESGP"
     gene            complement(809..1819)
                     /locus_tag="VIW27_05000"
     CDS             complement(809..1819)
                     /locus_tag="VIW27_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEY5739059.1"
                     /translation="MTLLVTGAAGFIGHALATRLCERGDEVIGIDNLNDYYAVTLKQA
                     RLRQLEGRDNFRFQRLDLADSAAVGALFDEHRFERVLHMAAQAGVRYSLENPRAYIDS
                     NLVGFGNLLECCRRHEVEHLVYASSSSVYGANRRLPYSTSDNVDHPVSLYAATKKANE
                     AMAHSYSHLFNLPCTGLRFFTVYGPWGRPDMSPILFADAIMQGRPIKVFNEGRHRRDF
                     TYIDDIAAGVLACLERVPEPDPDWSGLDPDPSVSSAPWKIYNIGRGEPVDLMHYIDLM
                     EQALGRTTTRQLLPAQPGDVEETWADIGPLVADTGYRPTISIEEGLQEFAVWYLDHYQ
                     KK"
     gene            complement(1877..2536)
                     /locus_tag="VIW27_05005"
     CDS             complement(1877..2536)
                     /locus_tag="VIW27_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEY5739060.1"
                     /translation="MPFAAALKSAWAKPLLHGLCLLPLATLLWAAFNGGLGANPVEKL
                     THETGDWTLRLLLLTLALSPLRQWTGIAAVIRFRRMLGLYTFFYLCGHFLIWFVADHS
                     LAITGMLEDIVERPYITLGFSGFVLMIPLALTSNRASLLRLGKRWKTLHQLVYPILLL
                     GVLHFIWLTRADYLEPGIYAIIAVILLLHRVGPVRRAVSGSPPVARRPEFGPDPRRMQ
                     Q"
     gene            complement(2538..3479)
                     /gene="msrP"
                     /locus_tag="VIW27_05010"
     CDS             complement(2538..3479)
                     /gene="msrP"
                     /locus_tag="VIW27_05010"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019590084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY5739061.1"
                     /translation="MIKIPKRWEIPESEVTPEPIYRRRREFIKGAGALGGAMLLNPLV
                     SAQASLRIDSYQPGVVTLDEELTSEEDATSYNNFYEFGTGKEDPKRNAQGFKTDDWSI
                     EVSGHCEKPGTFDIESLVRPHSIEERIYRLRCVEAWSMVIPWLGVPLGSILAGFQPTS
                     KAKYVAFKTLHDPVRMPGQQRSVLNWPYREGLTIAEAMHPLTLLAVGMYGKTMPNQNG
                     APIRLVVPWKYGFKSIKSIVSIEFTEEQPPTSWNIAAPREYGFYSNVNPEVDHPRWSQ
                     KRERRIGEIFKRDTLMFNGYEQEVVELYDHLDLTVNF"
     gene            complement(3590..>4372)
                     /locus_tag="VIW27_05015"
     CDS             complement(3590..>4372)
                     /locus_tag="VIW27_05015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY5739062.1"
                     /translation="IGAARPRVAILREEGVNGQLEMAAAFDRAGFDAHDVHMSDIIAG
                     RVSLADFQGLAACGGFSYGDVLGAGEGWSKSILYNQRARDEFSAFFERGDSFGLGICN
                     GCQMMSNLYRMIPGAGHWPRFVRNRSEQFEARVAMVEILDTPSIFLAGMPGSMMPVVV
                     AHGEGRIETRAHDAASLLEDGLACLRYVDAHGRPSETYPLNPNGSALGLNGFSSEDGR
                     FTIMMPHPERVFRSVQNSWRSPDWGEYGPWMRLFRNARHWID"
BASE COUNT          833 a         1372 c         1368 g          799 t
ORIGIN      
        1 ggtcccgact cgactacccg cccgtggtac atgaccacca cctggtcgga catgttggcg
       61 accaccccca ggtcgtgggt gatcaacagc aaggccatgc cgagctcggc ctgcaactcg
      121 cggatcagtt tgaggattcg cgcctggatg gtcacatcga gcgcggtggt cggttcgtcg
      181 gcgatcatca ggcagggatg gcagatcatc gccatcgcga tcatcgcccg ctggcgcagg
      241 ccgccggaca attcgaacgg atacatgcgc agcgcgcgcc gcgcgtcggg aaagccgacg
      301 tgctgcagca tcgcctcggt caggtgcatg ccctcggcgc ggctggcgtc gcggtgcagg
      361 aacagcgctt cgctgatctg atcgccgatg gtatgcaccg gcgacagcga agtcatcggc
      421 tcctggaaga tgatcgagat attaccgccg cgaatacggc gcatttgcgc ggattcggga
      481 tcgagccggg cgatgtcgac cagctcgccg gtttccgggt tatcgaagcg tatttcaccg
      541 ccacgaatga cggcgttggc gggcaatatg cccatgatgg cctgcgcggt aaccgattta
      601 cccgatcccg attcgccgac cagtgcgacc gactcgcctg gcgcgatcga gaacgaagcg
      661 ttggtcaccg cgggtaccgg attaccatgc agcaagaaat cgatcgcgag accgtttatt
      721 tcgagcaagg cagactgtga agcagcctct acgatgtcac accccttgag ccagttccct
      781 ttacgggatt attgcacgca attataaatt atttcttttg atagtgatct aaataccaga
      841 cggcaaattc ctgcaaaccc tcctcgatgg atatcgtcgg ccggtaaccg gtatccgcga
      901 ccagcgggcc tatatccgcc caggtttcct cgacgtcccc gggctgcgcc ggcagcagtt
      961 ggcgcgtcgt cgtacgcccc agcgcctgtt ccatcaggtc gatgtaatgc atcaggtcga
     1021 ccggttcgcc gcggccgatg ttgtagattt tccacggcgc gctgctgacc gacggatccg
     1081 ggtcgaggcc cgaccaatcg gggtcgggtt cgggaacccg ctcgagacag gcgagtacgc
     1141 cggcggcgat atcgtcgata taggtgaaat cgcggcgatg ccgaccctcg ttgaacacct
     1201 tgatcggtct gccctgcatg atcgcgtcgg cgaacaggat cggcgacata tccggccgcc
     1261 cccacgggcc atagacggta aagaagcgca ggccggtgca cggcaggttg aacaggtggc
     1321 tatagctgtg ggccatcgct tcattggctt tcttggtcgc cgcgtacagc gagaccgggt
     1381 ggtcgacgtt atcgctggtc gagtaaggca gccgccggtt ggcgccgtag accgaactgg
     1441 aagacgcgta caccaggtgc tcgacctcat gccggcggca gcattccagc aggttgccga
     1501 aaccgaccag gttgctgtcg atgtaggcgc gcggattttc cagcgagtaa cgtacgccgg
     1561 cctgcgcggc catatgcagc acgcgctcga atcgatgttc gtcaaacaac gcgccaacag
     1621 ccgccgagtc ggcgagatcg aggcgctgga agcggaaatt gtcgcgcccc tcgagctgcc
     1681 gcaggcgcgc ctgcttgaga gtgacagcat aataatcgtt caggttatcg atgccgataa
     1741 cctcgtcgcc gcgctcgcag agccgtgtcg cgagcgcatg gccaataaag ccggcggcgc
     1801 cggtaaccaa aagtgtcatt tcgtcttaat cacatgggcc gcggcggctt cccagcgcgc
     1861 cgtggacagg gtcgagttac tgctgcatgc gccgcgggtc ggggccgaac tcgggccgcc
     1921 gggcgaccgg gggcgacccc gacacggcgc gcctgaccgg ccccacgcgg tgcaacagca
     1981 ggattaccgc gataatcgca taaatgcccg gttcgagata gtccgcgcgg gtcagccaga
     2041 taaagtgcag tacgccgagc agcagaatcg ggtagaccag ctgatgcagg gttttccagc
     2101 gttttccgag gcgcagcaga ctcgcccggt tcgaggtgag cgccagcggg atcatcagca
     2161 caaagccgct gaaaccgagc gtgatgtagg gccgctcgac gatatcctcg agcatgccgg
     2221 tgatcgccag cgagtgatcg gcgacgaacc agatcagaaa gtgaccgcaa aggtagaaaa
     2281 acgtgtacag gccgagcatg cgccgaaagc gaatcaccgc ggcgatgccg gtccattgcc
     2341 gcagcggcga cagcgcgagc gtgagcagca gcaatcgcag cgtccagtcg ccggtttcgt
     2401 gggtcagctt ttcgaccggg ttggcgccga gcccgccgtt gaacgccgcc cacagcagcg
     2461 tcgccaacgg caacaggcaa agcccgtgca gcaatggctt cgcccaggcg gacttcagcg
     2521 ctgcggcgaa cggcatttca gaagttgacg gtcaggtcca ggtgatcgta aagctcgacg
     2581 acctcttgct cgtagccgtt gaacatcagc gtgtcgcgct tgaaaatttc gccgatccgg
     2641 cgttcgcgtt tctgcgacca gcgcggatgg tcgacttccg ggttgacgtt ggaataaaag
     2701 ccgtattcgc gcggcgcggc gatgttccac gaagtcggcg gctgttcctc ggtgaactcg
     2761 atgctgacga tcgacttgat gctcttgaag ccgtatttcc agggcaccac cagccgaatc
     2821 ggcgcgccgt tctggttcgg catggtcttg ccgtacatgc cgaccgcgag cagcgtcaac
     2881 gggtgcatcg cctcggcgat ggtcaggccc tcgcgatacg gccagttcag caccgagcgt
     2941 tgctggcccg gcatgcgtac cgggtcgtgc agggtcttga aggccacgta ttttgccttc
     3001 gaggtcggct gaaagccggc cagaatcgag cccagcggca cgcccagcca cggaatcacc
     3061 atcgaccagg cctcgacaca gcgcagccgg tagatgcgtt cctctatcga atgcggcctg
     3121 accaggctct cgatatcgaa ggtgccgggt ttctcgcaat gaccgctgac ctcgatgctc
     3181 cagtcgtcgg tcttgaaacc ctgtgcattg cgtttcggat cttccttgcc ggtaccgaac
     3241 tcgtaaaaat tattgtaact ggtggcgtct tcttcgctgg tgagttcctc gtcgagggtt
     3301 accacgccgg gctggtaact gtcgattcgc agcgaggctt gcgcgctgac cagcggattg
     3361 agcagcatcg ccccgccgag cgcgccggcg cccttgatga actcgcgccg ccggcggtat
     3421 atcggttcgg gggtaacctc gctttccggg atttcccagc gcttcggaat cttgatcatg
     3481 ttttcctcct gcaggatcct tctagacccg attatcggcg acgagttccc gtttacggct
     3541 cgcccacgcc agcaccaccc ccactcgccg gcagcgtaac cgcgttaatt cagtcgatcc
     3601 agtggcgcgc gttgcgaaac agccgcatcc acggaccata ctcgccccag tcgggcgagc
     3661 gccacgaatt ctgcacgctg cggaacaccc gctcggggtg cggcatcatg atcgtgaagc
     3721 ggccatcctc gctgctaaac ccgttcaatc ccagtgccga accgttgggg ttcagcggat
     3781 aggtctcgct cggccggcca tgcgcatcga catagcgcag gcaggcaagg ccatcctcga
     3841 gcaggctggc ggcgtcatgc gcgcgggtct cgatgcgccc ttcgccgtgg gcaaccacca
     3901 ccggcatcat cgatcccggc atgccggcaa ggaagatcga cggggtgtcg agtatttcga
     3961 ccatcgcgac gcgcgcctcg aactgctccg agcggttgcg cacgaagcgc ggccagtgcc
     4021 cggcgccggg aatcattcga tacaggttgg acatcatctg gcagccgttg cagataccga
     4081 gcccgaagct gtcgccgcgc tcgaagaacg ccgagaactc gtcgcgcgcg cgctggttgt
     4141 agagaatcga tttcgaccag ccctcgcccg cgccgagcac gtcgccatag gaaaagccgc
     4201 cgcaggccgc aagaccctga aaatcggcga gactcacgcg cccggcgatg atgtcgctca
     4261 tgtgtacgtc gtgcgcgtcg aagccggcgc gatcgaaagc ggccgccatt tccagctggc
     4321 cgttgacgcc ctcctcgcgc agaatggcga cgcgcggcct ggcagcgccg at
//
LOCUS       DATCZV010000225         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_758916, whole genome shotgun sequence.
ACCESSION   DATCZV010000225 DATCZV010000000
VERSION     DATCZV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758916"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VIW27_05020"
     CDS             <1..347
                     /locus_tag="VIW27_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5739063.1"
                     /translation="QLQHKLGERLGDEVVLVSVSVDPVRDTPQRLKAYSQIHQARPGW
                     IWLTGQKSTMNAVLDGLGAYSPNFEDHAAMVLIGDGQSGQWSRLYGFPNPDRIIERVN
                     ALQAARDAVSGG"
     gene            351..932
                     /locus_tag="VIW27_05025"
     CDS             351..932
                     /locus_tag="VIW27_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5739064.1"
                     /translation="MIKLKRTLTGFCLGLLLLPLAGHAQQSAEDKARNYFTDTQVIDQ
                     NGNKLRFYTDVLKERVVLISFMFMNCEGACPMLAQKLKQARAMMVPAIKDEVWYVTLS
                     VDPERDTPQAMKQFAENQGLDESRWLFLTGEKQNVELLIRKLGQYTPNIESHTTLMLA
                     GNDRTRHWTRVLPMVPPDGIAQQMRALVEETPG"
     gene            932..2512
                     /locus_tag="VIW27_05030"
     CDS             932..2512
                     /locus_tag="VIW27_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739065.1"
                     /translation="MGGQRIRQYLAPAALLLAFLFAPQAPAEPSAAESRGKQIYVEGS
                     SPSGGEITAIVGTEGVSLPASAVPCASCHGADGLGRREGGVIPPDIRWSELTKVYGHV
                     HEDGRKHQAFDEISIARLIRTGLDPDFNQLDRSMPLYNMSVEDMNSLIAYLRFLEQDL
                     DPGIGKDSIQFGTLLPLRGPRGELGQAMAQVMLAYFKDINQRGGVFGRRLELLAIPWG
                     DSAESTLDNLRSAFEQEGVFALVGAYTIGMDDQLLELVREQRMPMIGPFTLNPGDAFV
                     NQDVFYIYPGFAEQAQALAEQAIAQMKGARTNLVLVGPQGADVDGLIAAVEDRFPQDK
                     TFESRSLRYPRGQLDASALATDLQSNGGDAILFLGGQDELVTLLEALDSRQHYPGIFA
                     LSAFAPQLYEAPAAFNQRIYLAYPTLSRDVSAAGRSEYQRLAETYALPPGHLQGQVAA
                     LAAVKLLVEGLRGAGHSLDRARLVEAIEALYRYDTGFTPPLTYGPNRRIGALGAHVMV
                     VDIDNRTTRPVGEWRELR"
     gene            2810..>3520
                     /locus_tag="VIW27_05035"
     CDS             2810..>3520
                     /locus_tag="VIW27_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEY5739066.1"
                     /translation="MLSLVRPCNARNRLTTRVGYVLAAILLLGFVGPAPAEVTVSVDR
                     YPVRVNESFQLVFSNDVTPDREPDFSLLQPHFTILGNKRGSRTRIVDGEYQRRIDWTL
                     QLMPKRVGEATVPAVGFDDERSEPLKITVMPAVLTVVPRDELVLDLLADVDEAYVQGQ
                     VVLTLRLLSETDNSAYEFADLGVGSLDVVIEPLGGERQYQARIAGHRYLVRERQYALF
                     PQQSGRLEIGPALAETARY"
BASE COUNT          652 a         1068 c         1154 g          646 t
ORIGIN      
        1 gccagttgca gcataagctc ggcgagcggc tcggcgacga ggtggtgctg gtctcggtca
       61 gcgtcgaccc ggtcagggac acgccgcagc ggctcaaggc ctattcgcag atccaccagg
      121 cgcgaccggg ctggatctgg ctgaccggcc agaagagcac gatgaacgcg gtgctcgacg
      181 gcctcggcgc ctacagcccc aatttcgagg accacgcggc gatggtgctg atcggcgacg
      241 gccagagcgg ccagtggtca cgcctgtatg gtttccccaa tcctgaccgg ataatcgagc
      301 gggtcaatgc attacaggcc gcgcgcgatg cggtctctgg aggataaatt atgatcaagt
      361 tgaaacgaac actgaccggg ttttgcctcg gcttgctgtt gttgccgctg gccggtcacg
      421 cccagcagtc cgcggaagac aaggcgcgga attactttac cgacacccag gtgatcgacc
      481 agaacggcaa caagctgcgc ttctataccg acgtgctgaa ggagcgcgtg gtgctgatca
      541 gcttcatgtt catgaactgc gagggcgcct gcccgatgct ggcgcagaag ctgaagcagg
      601 cgcgcgcgat gatggtgccg gcgatcaagg acgaagtctg gtacgtgacg ctgagcgtcg
      661 acccggagcg cgacacgccg caggcgatga agcagttcgc cgagaaccag ggcctggacg
      721 aaagccgctg gctgttcctg accggcgaga aacagaacgt cgaattgctg atcagaaagc
      781 tgggtcagta cacgccgaat atcgagtcgc acaccacgct gatgctggcc ggaaacgacc
      841 gcacccggca ctggacccgc gtgctgccga tggtgccccc ggacggcatt gcccagcaga
      901 tgcgggcgct ggtcgaagag acgcccgggt aatgggcggg cagcgcattc ggcaatacct
      961 ggcgccggca gcgttgctgc tggcgttttt gtttgcgccg caggcgcccg cggaaccgag
     1021 cgcggcagaa agccgcggca agcagatcta tgtcgagggc agcagcccga gcggcggcga
     1081 gattaccgcg atcgtcggca ccgagggtgt cagcctgcca gccagcgcgg tgccctgtgc
     1141 cagctgtcat ggcgccgacg gactcggccg ccgcgagggc ggcgtgattc cgccggacat
     1201 tcgctggagc gaactgacca aggtctacgg ccacgtccac gaggacggcc gcaagcacca
     1261 ggcgttcgac gagatcagta tcgcgcggct gatcaggacc ggcctcgatc cggatttcaa
     1321 ccagctcgac cgctcgatgc cgctgtacaa catgtcggtc gaggatatga acagcctgat
     1381 agcctacctg cgtttcctcg agcaggacct ggatccgggc atcggcaagg acagcattca
     1441 gttcggcacg ctgctgcctt tgcggggtcc ccgcggcgaa ctcgggcagg ccatggcgca
     1501 ggtgatgctg gcctatttca aggatatcaa tcagcgcggc ggcgttttcg ggcgcaggct
     1561 cgaattgctg gcgattccct ggggcgactc ggccgagtcg acgctcgaca acctgcgctc
     1621 cgcgttcgag caggaaggtg tgttcgcgct ggtcggcgcc tacaccatcg gcatggatga
     1681 ccagcttctc gaactggtac gcgaacagcg aatgccgatg atcggcccgt ttacgctgaa
     1741 cccgggcgat gcattcgtca accaggatgt tttctatatt taccccggct ttgccgagca
     1801 ggcacaggcg ctggccgagc aggccatcgc gcaaatgaag ggcgccagga cgaacctggt
     1861 gctggtcggg ccgcaaggcg cggacgtcga cggcctgatc gcggcggtcg aggaccgatt
     1921 tccgcaagat aagaccttcg agtcgaggag cttgcgttac ccgcgcggcc aactcgatgc
     1981 aagcgcgctg gcgaccgacc tgcaaagcaa tggcggtgat gccatcctgt ttctgggcgg
     2041 gcaggacgaa ctcgttaccc tgctcgaggc gctggattcc cggcagcact acccgggtat
     2101 tttcgcgctg tcggccttcg ccccgcagtt gtacgaggca cccgcggcct tcaaccagcg
     2161 tatttacctg gcctatccga cgctgtcgag agatgtcagt gctgccggcc gcagcgagta
     2221 ccagcgactg gcggagacct acgctctgcc gcccggccac ctgcaggggc aggtggcggc
     2281 gctggccgcc gtcaaactgc tcgtggaggg cctcaggggc gccggccaca gtctcgaccg
     2341 cgcgcgcctg gtcgaggcca tcgaggcgct gtaccgctac gatacgggtt tcacaccgcc
     2401 gctgacctat ggccccaacc gccgcatcgg cgcgctgggc gcgcacgtga tggtggtcga
     2461 tatcgacaac cgcacgaccc ggccggtggg agagtggcgt gagctcagat aatcatctgg
     2521 ggtcggacca ggttaagcgt ctgattttat tcaacttcag tgggttcttg gtttgttttc
     2581 agtccagttg gggtcggacc aaggtaacca gttgatttta ttgaatttaa gccatttttt
     2641 ggcctgtttc agggtttaaa atgacgattt ttccagtgaa aaatatacct tggggagaat
     2701 agacgcataa accgggacgg ctttcgcgag aggcagcatt tttcgcgcaa aatgcccttg
     2761 ccaggcctga ttccgggcca gttttctgcc gaaattcttt ttttatcaga tgcttagcct
     2821 ggtccgaccc tgcaatgccc gcaaccgctt aacgacgcgg gtcggctacg ttctcgccgc
     2881 aatattgttg ctcggtttcg tcgggccggc gccggcggag gtaacggtca gcgtcgatcg
     2941 ctacccggtg cgggtcaacg aatccttcca gctggtgttt tcaaacgacg ttacgccgga
     3001 tcgcgagccc gatttttcgc ttctgcagcc gcacttcacg atcctcggca ataagcgcgg
     3061 cagccgcacc cgcatcgtcg atggcgagta tcagcgcaga atcgactgga cgctgcagct
     3121 gatgccgaag cgggttggcg aggccaccgt gccggcggtc ggcttcgacg acgagcgcag
     3181 tgaaccgcta aagatcacgg ttatgccggc ggtcctgacg gtcgtgccgc gggatgaact
     3241 ggtgctggac ctgctcgccg acgtcgacga ggcctacgtt cagggccagg tcgtgctgac
     3301 gctgcgcctg ctgagcgaga ccgataattc ggcttacgag ttcgccgacc tcggggtcgg
     3361 ctcgctggac gtggtgatcg aaccgctggg cggcgaacgc cagtaccagg cgcgcatcgc
     3421 cggccaccgt tacctcgtgc gggagcggca gtacgcgctg tttccgcagc aaagcggccg
     3481 cctcgagatc ggaccggcgc tggccgagac ggcgcgctac
//
LOCUS       DATCZV010000226         5131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_123218, whole genome shotgun sequence.
ACCESSION   DATCZV010000226 DATCZV010000000
VERSION     DATCZV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123218"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VIW27_05040"
     CDS             <1..1619
                     /locus_tag="VIW27_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5739067.1"
                     /translation="ATVATLVVIVLSSIVGMGIAGYAGVKLTPISMSAPIVILTLAVA
                     DSIHILLTLRNLLRTGMDKAAALVEAVRLNFLAVGITSLTTIVGFLALNFSDSPPYWH
                     LGNITAVGIGAAWLYSVTLFPALLALLPYRVARAQGPAWSLRAMDRFADWVIANHRRL
                     LIGVGAGALALIAFIPRIEFNDQWVQYFDERIEFRNDSDNAEAFFGLYPIEFSVPAAS
                     AGGVSEPEYLQHLDQFADFLRTRPIVSHVYSLSDIMRRLNRNMHGDDRAYHRIPDDRD
                     LSAQYLLLYELSLPYGLDLNDRINIDKSASRVTATLTRATTAETRAFFREVAGWQQAN
                     WPAYMYAEPTSAAVMFTYISQRNMENMVTGTIIAIVAISLIMVFALRSVSLGLLSLVP
                     NGLPILATFGAWALLVGTVGFSVATVASIALGIIVDDTVHLLSKYVRARDERKLSIED
                     SIRYAFHNVGGAIIVNTIVLSAGFMVMMTSAFKMNVDLGLMTVLSIVFALILDFLLLP
                     ALLMLGVRKRDAAASQPLPGEPSMISVSFN"
     gene            1644..2447
                     /locus_tag="VIW27_05045"
     CDS             1644..2447
                     /locus_tag="VIW27_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY5739068.1"
                     /translation="MKHLPRNTLASLLAAVLALGSAAAASTDVEQGFAIAARADRSDV
                     GFGNSEVELQMILRNAAGEESTRRLRISTLEKPDESVGDKSLVLFDTPADIKGTALLS
                     HARILDSDDQWLYLPALKRVKRISSRNKSGPFVGSEFAFEDFTAIELNKFDYRYIGTE
                     ACGEFECDLVERLPRYQNSGYKRQLSWIDRTHYQLRKVEFYDRRGDLLKVLELNDYRR
                     YDNGTWRAHRLSMNNMQTNKQTELVYGDYRFAVDIGERDFESAGLKRLR"
     gene            complement(2533..2733)
                     /locus_tag="VIW27_05050"
     CDS             complement(2533..2733)
                     /locus_tag="VIW27_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739069.1"
                     /translation="MIFETEAERVREFVRDGNYHAAYNIALSAMNACRRDGDQSGVDA
                     CIALIREIVDSLADEFGGEQAD"
     gene            complement(2761..3849)
                     /locus_tag="VIW27_05055"
     CDS             complement(2761..3849)
                     /locus_tag="VIW27_05055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5739070.1"
                     /translation="MVRLLLALGLLCWDSAATAGARAEIPLTEATPQSQGLDGARLQQ
                     AVDGIAAGDYGRIDALLVLRNDFIVLERYFSPLYRGPDYRYPVRSVTKSITSALVGIA
                     RGQGKLPALQTGLRELFPQYSSIANLDTRKRAVTFEHLLSMTAGFAWDELSPGAMSDS
                     AMVSSPDWIKHVLDLPMHDLPGERLVYNSGCSMLLSDILLRGTGMAVDEFAARYLFEP
                     IGIDNWRWSLGKNNVINTAYGLSMTRRDMARFGTLYLNRGNWRGRQVVPEDWVGLSTA
                     TRVLGSGENYPYAYAYQWWRLQDGEPTAAMLATNDLYTALGMGGQFITVIPHLDMVVV
                     STAENYAPANEELLLKLLRDHIIPAVSR"
     gene            3997..4803
                     /locus_tag="VIW27_05060"
     CDS             3997..4803
                     /locus_tag="VIW27_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5739071.1"
                     /translation="MTKLLYEVVDGIAWIRFNKPEILNAIDAEETRRFVELLRESSND
                     PDVKAVIVSSVGEAFCVGDDLKVAMDEYPKILAGEIHPILDIVEDITENLQQIARVMR
                     DSPKVCISAVRGYAVGAGFEIAIDSDLIVAADNAVFGFPEMNAGMTVTGGASKLLASI
                     VGLNRARELMLTGEFIDAEEAWRIGLVNRLVKPGEEEKAAEKLARIVMSRAPLAIMQH
                     KRLLAQAAEAGFETVLNLEKQTISNLIFSEDYGEALKAFAEKRKPEFKGR"
     gene            4800..>5131
                     /locus_tag="VIW27_05065"
     CDS             4800..>5131
                     /locus_tag="VIW27_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5739072.1"
                     /translation="MSKLEIETTENRDLPTVLANRVAHCPDKPWILTADKSWSYREMD
                     EQSGRLAQGMVECGIAHGDTVLIMLPDTVDYILAWCALSKFGGIEVPVNVHNRGNPFA
                     YLVNDSLA"
BASE COUNT          964 a         1665 c         1547 g          955 t
ORIGIN      
        1 cggcgaccgt tgcgacgctg gtggtcatcg tgctgtcgtc gatcgtcggc atgggcatcg
       61 ccggctacgc gggcgtcaag ctgacgccga tctcgatgtc ggcgccgatc gtgatcctga
      121 cgctggcggt ggccgactcg atccatatcc tgctgacgct gcgcaacctg ctgcgcacag
      181 gcatggacaa ggccgcggcg ctggtcgaag ccgtccggct caacttcctc gccgtcggta
      241 ttacctcgct gaccaccatc gtcggtttcc tggcgctcaa cttttccgat tcgccgcctt
      301 actggcacct cggcaacatc accgcggtgg gtatcggcgc cgcctggctg tactcggtga
      361 cactgttccc ggcgctgctc gcgctgctgc cctaccgcgt agcgcgggcg cagggcccgg
      421 cctggtcgct gcgcgcgatg gaccgtttcg cagactgggt gatcgccaat caccggcggc
      481 tgctgatcgg ggtcggcgcc ggcgcgctgg cattgatcgc cttcattccg cgcatcgaat
      541 tcaacgacca gtgggtgcag tacttcgacg agcgtatcga gtttcgcaac gactcagaca
      601 atgccgaggc attcttcggc ctttacccga tcgaattctc ggtaccggcg gcatcggcgg
      661 gcggcgtcag cgaacccgag tacctgcaac acctcgacca gttcgccgat ttcctgcgca
      721 cccggccgat cgtctcgcat gtctattcgc tgtccgacat catgcgccgg ctaaaccgca
      781 acatgcacgg cgacgacagg gcataccacc gtattccgga cgaccgcgac ctgtccgcgc
      841 aatacctgct gctttacgaa ctgtcgctgc cctacggtct cgacctcaac gatcgcatca
      901 atatcgacaa gtccgcgagc cgcgtaaccg cgacactgac ccgcgcaacc accgccgaaa
      961 cccgcgcttt tttccgcgaa gtcgccggct ggcagcaggc caactggccg gcctacatgt
     1021 acgccgagcc gaccagcgcg gcggtgatgt tcacctacat cagccagcgc aacatggaaa
     1081 acatggtcac cggcacgatc atcgccatcg tcgcgatctc gttgatcatg gtatttgcgt
     1141 tgcgcagcgt atcgctcgga ttgctgagcc tggtgcccaa cggcctgccg atcctggcga
     1201 ccttcggcgc ctgggcgctg ctggtcggca cggtcggttt ttcggtggcc acggtagcgt
     1261 cgatcgcgct cggcatcatc gtcgacgata ccgtgcacct gttgtcgaag tacgtgcgcg
     1321 cgcgcgacga acgcaagctt tcgatcgagg actcgattcg ctatgccttt cataacgtcg
     1381 gcggcgcgat catcgtcaac accatcgtgc tcagcgccgg tttcatggtg atgatgacct
     1441 cggccttcaa gatgaacgtc gatctcggcc tgatgaccgt gttgtcgatc gtgttcgcgc
     1501 tgattctcga ttttctgctg ctgcccgcgc tgctgatgct tggcgtgcgc aagcgcgatg
     1561 ccgcggcatc gcaaccgctg ccgggcgaac ccagcatgat cagcgtcagt tttaactaaa
     1621 ccttttttac aggagataac accatgaaac atctaccccg taataccctc gcatccctgc
     1681 tcgccgccgt gctcgcgctc ggcagtgcgg cggcggccag caccgacgtc gagcagggct
     1741 ttgcaatcgc cgcgcgcgcg gatcgcagcg acgtcggctt cggcaacagc gaagtcgagc
     1801 tgcagatgat cctgcgcaac gccgccggcg aggaatcgac gcgcaggctg cgcatttcga
     1861 cactggaaaa gcccgacgaa agcgttggcg acaagagcct ggtgctgttc gacacgccgg
     1921 ccgatatcaa gggtaccgcg ctgttgtcgc acgcacgcat cctcgactcc gacgaccagt
     1981 ggctctactt gccggcactg aagcgcgtca agcgcatttc gtcgcgcaac aagtccggtc
     2041 cgttcgtcgg ttccgagttc gcgttcgagg atttcaccgc gatcgagctc aacaagttcg
     2101 actaccgcta catcggcacc gaggcctgcg gcgaatttga atgcgacctg gtcgagcgcc
     2161 tgccgcgcta tcagaactcg ggttacaagc gccaactctc gtggatcgac aggactcact
     2221 accagttgcg caaggtcgag ttctacgacc gtcgcggcga cttgctgaaa gtgctcgagc
     2281 tgaacgacta ccggcgttac gacaacggta cctggcgcgc gcatcgcctg agcatgaata
     2341 acatgcagac caacaagcag accgagctgg tatacggcga ttatcgcttc gcggtcgata
     2401 tcggcgaacg cgatttcgag agcgctggtc tcaagcgcct gcgctagggc ggatcgagca
     2461 ttcctctgcg gttgcgcctt gccggcgttc gggcgccgtc ctcaaacgat gcccgaacgc
     2521 tggcggcagc ggtcaatccg cctgctcgcc accgaactcg tcggccagcg agtcgacgat
     2581 ctcgcgtatc agcgcgatac aggcgtcaac cccggactgg tcgccgtcgc gacggcaggc
     2641 attcatcgcc gacaacgcga tgttgtaggc agcatggtaa ttgccgtcgc ggacgaattc
     2701 gcgtacccgc tcggcctctg tttcgaaaat catttcagat tctccggtgg ttgatggcga
     2761 tcatcgcgat acggcgggaa taatgtggtc gcgcaacagc ttcaacagca actcctcgtt
     2821 cgcgggagcg taattttccg cggtcgaaac caccaccatg tcgagatggg gaatgaccgt
     2881 gatgaactgg ccgcccatac cgagcgcggt atacaggtca ttggtcgcca gcatcgctgc
     2941 ggtcggctcg ccgtcctgca ggcgccacca ctggtaggca taggcgtagg ggtaattttc
     3001 gccgctgccg agcaccctgg tagcggtcga caagccgacc cagtcctcgg gtacgacctg
     3061 tcggccccgc cagttgccgc ggttcaggta gagcgtgccg aagcgcgcca tgtcgcgtcg
     3121 cgtcatcgac aggccgtagg cggtgttgat gacgttattc ttgcccagcg accagcgcca
     3181 gttgtcgatg ccgatcggct cgaacagata cctggcagcg aattcatcta ccgccatgcc
     3241 cgtaccgcgc agcagtatat ccgacagcag catcgagcaa ccgctgttgt acaccagtcg
     3301 ctcgccgggc aggtcgtgca tcggcaggtc gagcacgtgc ttgatccagt ccgggctgct
     3361 caccatcgcc gaatcgctca tcgcaccggg ggaaagctcg tcccaggcaa agccggcggt
     3421 catcgacagc aggtgttcga aggtcacggc gcgcttgcga gtatcgaggt tggcgatact
     3481 cgaatactgc ggaaacagct cgcgcagccc ggtctgcaga gcgggcagtt taccctggcc
     3541 ccgcgcgatg ccgaccagcg ccgaggtgat gctcttggta accgagcgaa ccgggtagcg
     3601 gtagtccggg ccgcggtaga gcggcgaaaa gtaacgctcg agcacgatga agtcgttgcg
     3661 caataccagc agtgcgtcga tcctgccgta gtcgccggcc gcgatgccgt cgacggcctg
     3721 ctgcaggcgc gcgccgtcga gcccctgcga ctgcggcgtc gcctcggtca gcggtatttc
     3781 ggcccgggcg ccggcagttg ccgcgctatc ccagcacagc aggcccagcg ccaatagcaa
     3841 cctgaccatt tccgacagtc ccatcctgga tatcacccgc cattataccc gaaggccgcc
     3901 gggcccgctt cgcggaccgc gagttgacaa atgcccgtgc atggcgctag cgttttgttt
     3961 ctttcaccga ccgccgcgaa tgcgacaaac gagcctatga ccaagctgct gtacgaagta
     4021 gtcgacggga tcgcctggat acgcttcaac aagccggaaa tcctcaacgc gatcgacgcc
     4081 gaggaaaccc gccgcttcgt cgagttgctg cgcgagtcgt cgaacgaccc cgatgtcaag
     4141 gcagtcatcg tctccagcgt cggcgaggct ttctgcgtcg gcgacgacct caaggtagcg
     4201 atggacgagt atccgaagat cctcgccggc gagattcacc cgatcctgga tatcgtcgag
     4261 gatatcaccg agaacctgca gcaaatcgcg cgcgtgatgc gcgactcccc caaggtctgc
     4321 atttcggcgg tgcgcggcta cgcggtcggc gccggtttcg agatcgccat cgattcggac
     4381 ctgatcgtcg ccgccgacaa tgccgtgttc ggctttcccg aaatgaacgc gggcatgacg
     4441 gtgaccggcg gtgccagcaa gctgctggcg tctatcgtcg gcttgaaccg cgcgcgcgaa
     4501 ctgatgctga ccggcgaatt catcgatgcc gaggaagcct ggcgcatcgg cctcgtcaac
     4561 cggctggtga agccgggcga agaagaaaag gctgccgaaa aactcgcgcg tatcgtgatg
     4621 tcgcgcgcgc cgctggcaat catgcagcac aagcggctgc tcgcgcaggc cgccgaggcc
     4681 ggcttcgaaa ccgtgttgaa cctggagaag cagacgatct ccaacctgat cttcagcgag
     4741 gattacggcg aggcgctcaa ggctttcgcc gaaaaacgca aacccgagtt caagggcaga
     4801 tgagcaaact cgaaatcgaa accaccgaaa accgcgacct gccgacggtg ctcgccaacc
     4861 gcgtcgcgca ctgccccgac aagccgtgga tactgaccgc cgacaagtcc tggagctacc
     4921 gcgagatgga cgaacagtcg ggccggctcg cgcagggcat ggtcgagtgc ggcatcgccc
     4981 acggcgacac ggtgctgatc atgctgccgg acacggtcga ttacattctt gcctggtgcg
     5041 ccttgtcgaa gttcggcggc atcgaggtac cggtcaacgt gcacaaccgc ggcaacccct
     5101 tcgcttacct ggtcaacgat tcgctggcca a
//
LOCUS       DATCZV010000227         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_706382, whole genome shotgun sequence.
ACCESSION   DATCZV010000227 DATCZV010000000
VERSION     DATCZV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706382"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..150
                     /locus_tag="VIW27_05070"
     CDS             13..150
                     /locus_tag="VIW27_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739073.1"
                     /translation="MPVFASIMAVIFLGEAFRLYHALALFLVLGGIGLSELGKPQPAD
                     K"
     gene            complement(159..1061)
                     /locus_tag="VIW27_05075"
     CDS             complement(159..1061)
                     /locus_tag="VIW27_05075"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5739074.1"
                     /translation="MMQNAEWKIAPPVMLEQLVGRGQELPSMPEIYMRVSELLENDNS
                     SLQQIADTVQNDPAITSRVLKMVNSSYYGLPSQITSVAQAVVLLGRERLRHLLIGSVL
                     RGVFIEHDNPAFSMLAFWQHSIKTAIIARQLAAEIEQIEEPDAMFTAGLLHDIGKLLL
                     INRFPERMLAAEEYMMRKRADVLTAELIQMGVTHTAVGEALMQYWGLPQLLVDCASRH
                     HESVHDGHNRYATHLIYLASHLSNYVPPLDEQETRDILDDIQNWDMGRLSLEQIGSAC
                     QQADELVFEVMESLGMVAMEISGD"
     gene            1130..1468
                     /locus_tag="VIW27_05080"
     CDS             1130..1468
                     /locus_tag="VIW27_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739075.1"
                     /translation="MERRRSLDEALKNVTQIMFHRAIAACFTLPGHLPAASRLPLPTG
                     GPPLAEPRSNHGVEIRAMRRCWLRLEGLIACISLAKQEPMVALWPAADVKYPAVSFSD
                     NQFAACRKIR"
     gene            complement(1583..>2642)
                     /locus_tag="VIW27_05085"
     CDS             complement(1583..>2642)
                     /locus_tag="VIW27_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5739076.1"
                     /translation="GDGIGVDVTAAALAVVDAALARQSRLRLSYREIHAGAGYFRETG
                     QDIEPGGEAAAGQAEAIFLGAIGLPRVRHADGTEISPHLRLREIYQLYAGVRPVRAYP
                     NVPQRLADPRAAGIDLVILRESTEGLFYSAAVHGRSQVIGDREVRDTLRITRATTEKL
                     HRFGFELARKRRQRGRNGALTCVDKANVFTSMAFFRRIFDEIAAEYPDVHTDYNYVDA
                     QALDLVRRPWEFDVLVTENMFGDILSDLAGGLVGGMGMAACAEIGDHNGLFQPAHGSA
                     PDIIGQDLANPLAAVLSAALMLEYLADRSGEAGFADAAQAIEDAVYHGFEECRLRPAE
                     FGGDMGTRALTAALLELL"
BASE COUNT          483 a          831 c          821 g          507 t
ORIGIN      
        1 ttcgtcaacc tggtgccggt gttcgcatcg atcatggcgg tgatttttct cggcgaggcg
       61 tttcggcttt accacgcact ggcactgttc ctggtgctcg gcggtatcgg cctgtcggag
      121 ctcggcaagc cacagccggc cgacaagtaa attcggcttc agtcgccgct gatttccatc
      181 gccaccatgc cgagcgactc catgacctcg aaaaccagtt catcggcctg ctggcaggca
      241 ctgccgatct gctccagcga cagccgcccc atgtcccagt tctgaatgtc gtcgagaata
      301 tcgcgggttt cctgctcgtc gagcggcggc acgtaattgc tcaggtggct ggccaggtag
      361 atcaggtgcg tggcatagcg gttatggccg tcgtgtaccg actcgtggtg cctgctggcg
      421 caatcgacca ataactgggg caggccccag tactgcatca acgcctcgcc gaccgcggta
      481 tgagtgacgc ccatctggat cagttcggcg gtcagcacgt cggcgcgctt gcgcatcatg
      541 tattcctcgg ccgccagcat gcgttccgga aaacggttta tcaacagcag tttgccgatg
      601 tcatgcagca ggccggcggt gaacatcgcg tcgggttcct cgatctgctc gatctcggcg
      661 gccagctggc gggcgatgat cgcggtcttg atgctgtgtt gccaaaatgc cagcatcgag
      721 aaggcgggat tatcatgttc aatgaaaaca ccgcgcagta ccgagccaat cagcaaatgc
      781 ctgagacgct cacgaccgag cagcaccacc gcctgagcca ccgaggtgat ctggctgggc
      841 aggccgtaat agctactgtt gaccatcttc agcacgcggc tggtgatcgc cggatcgttt
      901 tgcacggtgt cggcaatttg ctgcaggctg ctgttatcgt tttcgagcaa ttcggaaact
      961 cgcatgtaga tttccggcat gctgggtaat tcctggccgc ggccgacgag ctgctcgagc
     1021 atcaccggag gggcgatttt ccattcggcg ttttgcatca tgagaatgag catagcagag
     1081 aaattttttt gcatagcgcc gaatatctga cggccccgac gaggctgcca tggagcggcg
     1141 acgtagcctt gacgaagcac taaaaaatgt gacgcagatc atgttccacc gcgcgattgc
     1201 ggcctgtttt accctgcctg gccatcttcc tgcagcgagc cgactaccgc tgccgacagg
     1261 cgggccgccg ctggccgaac cgcggagcaa ccatggcgtc gaaattcgcg caatgcgccg
     1321 atgctggctg cgtctggaag gcctcattgc ttgcataagc ctcgcgaagc aggagccgat
     1381 ggtcgccttg tggcccgctg cggacgtcaa atacccggcg gtcagtttta gcgacaatca
     1441 attcgccgcc tgtcggaaaa tacgctgatg attgggtcgg cccggccaga gtgcgggcaa
     1501 aaccggcgat aaccggtgtc gattgcttcg gcttacagct gcgaacgccg cgactcgcgg
     1561 aagaataccg acggcacgcg gttcacaaca gctcgagtaa cgctgcggtc agcgcgcgcg
     1621 tgcccatgtc gccgccgaac tccgctggcc gcagccggca ctcctcgaat ccatggtaca
     1681 cagcgtcctc gatcgcctgc gccgcgtcgg caaagccggc ttcgccgctc ctgtcggcca
     1741 ggtattcgag catcagcgcg gcgctcagaa ccgccgccag cgggtttgcc aggtcctgcc
     1801 cgatgatatc cggcgcgctg ccgtgggctg gctgaaacag cccgttatgg tcgccgatct
     1861 cggcacaggc cgccatgccc atgccgccga ccaggccgcc ggcgaggtcg gagaggatat
     1921 cgccgaacat gttctcggtc accagcacgt cgaactccca cgggcggcgc accaggtcca
     1981 gcgcctgcgc gtcgacatag ttgtaatcgg tgtgcacatc cgggtattcg gcggcgattt
     2041 cgtcaaatat tcgccgaaag aaagccatcg acgtgaacac gttggccttg tcgacgcagg
     2101 tcagggcacc gttacgtccg cgctgcctgc gcttgcgcgc cagttcaaag ccgaaacggt
     2161 gcagcttctc ggtggtggcg cgcgtgatcc tcagcgtgtc gcgtacctcg cgatcgccga
     2221 tgacctggct gcggccatgc accgcggccg agtagaacag gccctcggtg gactcgcgca
     2281 gaatcaccag gtcgatgccg gctgcgcgcg gatcggcaag ccgctgtggc acattcgggt
     2341 aagccctgac cgggcgcacg ccggcatata actggtagat ttcccgcagc cgcaggtgcg
     2401 gcgagatctc ggtaccgtca gcatggcgca ctcgcggcag gccgatggca ccaagaaata
     2461 tcgcctcggc ctggccggcc gcggcttcgc cgccgggctc gatatcctgc ccggtttcac
     2521 gaaagtagcc ggcgccggcg tggatttcac gataactcag gcgcaaacgg gattgccggg
     2581 ccagcgccgc gtcgacgacc gcgagcgcgg cggcggtgac gtcgacaccg atgccgtcgc
     2641 cg
//
LOCUS       DATCZV010000228         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_600502, whole genome shotgun sequence.
ACCESSION   DATCZV010000228 DATCZV010000000
VERSION     DATCZV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600502"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VIW27_05090"
     CDS             complement(<1..1503)
                     /locus_tag="VIW27_05090"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEY5739077.1"
                     /translation="MIRYQVSCPVPASHYFAVVLRIDKPDPAGQALRLPNWIPGSYMI
                     RDFARNLLDLRAHAGGIEVALEQLDKSNWRAAPCDGELTVEYRVYAKDLSVRSAYLDH
                     TRCYYNGSSLFLEPLGLSQQACEVLIEKPADPRYADWRLATSLPRKQAGPFEFGLYEA
                     ADYDELIDHPVEMGDFTVIPFEACGVPHDIVLAGRFECDGERLAADLRAICEQHIRFF
                     GEPAPMDYYQFQVLIVGDGYGGLEHRSSTSLVARRASLPQAGAAGVSDDYREFLGLCS
                     HEYFHTWNVKRIKPAAYQPYDLQREVYTELLWAFEGITSYYDDLALRRAGLIDAPSYL
                     ELLAQTMTRVQRGLGRSRQSVAESSFNAWTKFYQQDENAANAIVSYYAKGSLIAACID
                     LKMRLLSGGETSLDDLMRRLWSDYLQSREGIAQDRIRQLAAELCGAEFGSFIDELIHG
                     TDDPPLPQLLAEAGIEVTRRAPANAQDKGGKPFTGELPRADFGALLQAAEG"
     gene            1581..2144
                     /locus_tag="VIW27_05095"
     CDS             1581..2144
                     /locus_tag="VIW27_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY5739078.1"
                     /translation="MNDSLKHLQRQSTPAKALAEPAPDDRQLRKILKTALTAPDHGRL
                     HPYRFISIRGDARHRLSEVFGAATRRRDPDVDPAYLKKQQDKPLRSPLILVVVAQLID
                     SPKIPEIEQMLSAGAAAHNVLLAANALGFGSIWLTGDNAYDAHVCDELGLADNERIIG
                     FIYIGTPVIELPPRPIPEVSTHHSEWK"
BASE COUNT          422 a          754 c          751 g          400 t
ORIGIN      
        1 gccctcggcc gcctgcagca gcgcaccgaa atcggcgcgc ggcagttcgc cggtgaacgg
       61 tttgccgccc ttgtcctggg cgttggcggg cgcgcgccgc gtaacctcga tgccggcctc
      121 ggccagcagt tgcggcagcg gcgggtcatc cgtgccgtga atcagttcgt cgatgaaact
      181 gccgaactcg gcaccgcaga gttccgcggc cagctgccga atgcggtcct gcgcgatacc
      241 ttcgcggctt tgcaggtaat ccgaccagag gcggcgcatg aggtcgtcga ggctggtctc
      301 gccgccgctc agcaggcgca tcttgaggtc gatgcaggcg gcgatcaggc tgcccttggc
      361 atagtaactg acgatcgcgt tggcggcgtt ttcatcctgc tggtagaact tggtccaggc
      421 attgaagctc gactcggcca ccgactggcg gctgcggccg aggccgcgct gtacgcgggt
      481 catggtttgc gccagcagct cgaggtacga aggcgcgtcg atcaggccgg cgcggcgcag
      541 cgcgagatca tcgtaatagg aggtgatccc ttcgaaggcc cacaacagct cggtatagac
      601 ttcccgctgc aggtcgtagg gttgataggc cgccggcttg atgcgcttga cgttccaggt
      661 atggaaatac tcgtggctgc acaggccgag gaattcgcgg taatcgtcgc tgaccccggc
      721 agcgccggct tgcggcaggc ttgcgcggcg cgccaccagg ctggtgctgg agcggtgctc
      781 gaggccgccg tagccgtcgc cgacgatcag cacctggaac tggtagtaat ccatcggcgc
      841 gggctcgccg aaaaaccgaa tgtgctgctc gcagattgca cgcagatcgg cggcgaggcg
      901 ttcgccgtcg cactcgaatc gcccggcgag cacgatatcg tgcggcacgc cgcaggcctc
      961 gaacggaatc accgtgaagt cgcccatttc caccgggtgg tcgatcagct cgtcgtaatc
     1021 cgccgcctcg tagagcccga actcgaacgg cccggcctgc ttgcgcggca gcgaggtcgc
     1081 cagccgccaa tcggcatatc gcgggtccgc cggtttttcg atcagcacct cgcaagcctg
     1141 ctgcgacaga cccagcggct cgagaaacag gctgctgccg ttgtagtaac agcgggtatg
     1201 gtcgagatag gcgctgcgca ccgacaggtc cttcgcgtat acccggtact cgacggtgag
     1261 ctcgccgtcg cagggcgcgg cacgccagtt cgacttgtcg agctgctcga gcgcaacctc
     1321 gatgccgccc gcgtgggcgc gcaggtcgag caggttgcgc gcgaagtcgc gaatcatgta
     1381 actgcccgga atccagttcg gcaggcgcag ggcctggccg gcggggtccg gcttgtcgat
     1441 gcgcaagacg acggcaaaat agtggctggc gggaacgggg cagctgacct ggtatctgat
     1501 cattcggatt tgttacgcaa aaatcgaggc cgaagtgtat cattggcgga ccggacgcca
     1561 aatcgaatta gccccttgtt atgaatgact cgctgaaaca cctgcagcgc caatcgacgc
     1621 cggccaaggc cctggccgag cccgctcccg acgacaggca attgcgcaaa atcctcaaaa
     1681 ccgcgttgac ggcgcccgat cacggccgcc tgcatcccta ccgcttcatc agcattcgcg
     1741 gcgatgcgcg ccaccggctc tccgaggtat tcggcgcggc gacgcggcgg cgcgacccgg
     1801 acgtcgaccc cgcctatctg aaaaagcagc aggacaagcc gttgcgctcg ccgttgatcc
     1861 tggtggtggt cgcgcaattg atcgacagcc cgaagattcc cgagatcgag caaatgctgt
     1921 cggccggcgc ggccgcacac aacgtgttgc tggctgccaa cgcactcggc ttcggctcga
     1981 tctggctgac cggcgacaat gcctacgatg cacacgtctg cgacgaactc gggctggccg
     2041 acaacgagcg tatcatcggc ttcatctaca tcggcacccc ggtgatcgaa ctgccgccgc
     2101 ggccgattcc cgaagtctcg actcaccaca gcgagtggaa gtagctttct ttacccgcct
     2161 tcccggcagc gctcgaagta ccctagatta accgtcgccc cggcccgcga gccggggccc
     2221 atcgcaagag ccgaacaggc actgcgcgaa gcactgctcc ctgctctcgc gcagtatccg
     2281 acaaagtttg aaaagattgc ggggcgagcc cgcaatgacg gcggtta
//
LOCUS       DATCZV010000229         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_229839, whole genome shotgun sequence.
ACCESSION   DATCZV010000229 DATCZV010000000
VERSION     DATCZV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229839"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1984)
                     /locus_tag="VIW27_05100"
     CDS             complement(<1..1984)
                     /locus_tag="VIW27_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739079.1"
                     /translation="MMRRILIASSVALVLAAAGAGWLMNTESGLRWAWRTTASWLPGD
                     LQAASVSGVLAGPIELSAASFADGELTLAAGRVELDWNPWALAAFTLEIDSLRIDSLR
                     IALPAANPDPQAEPRPFTAPEIRLPLKLRLEALELRAIEIARGDSAWRLERLGGNLYA
                     DGDRFEFGNLQLQSEFLDAGLSGHIATGDAWDHQVDFEWKTVLASGADLSGTGKLSGD
                     LAATRLEQQLDGALRMRQLLELEDLPGELRWRSRIDVAQFDTRLLDASLPELDGSLAA
                     MLQGDLESWAAEGNLKTVLPRLGAVSADFDLRGLEGERRFDGMRVEDFVLHALDGEIG
                     ARGEIAWAPVPAWEAEISLREIDPAGLDSEWPGRLNGSITTRGAWQADALTASARIVR
                     VDGELRGYPVAARGSGEWRRQGLSIEALEIDSGGTRLTASGRADDTLDLSWALHSDDL
                     AEFYPGARGDIDLEGMVRGTRDAPRIEASLAAEALEYADYRVGKITGKASVEPLQAER
                     FDIDLDAEDLRIGLREFERVTVRADPKRIDAHLVSILGSTDIALAGSLDGAAWRGMLV
                     GLTVETEEFSNWKLSAPAAITLSPEYSSLARSCLLGDNQGRVCSAWSGNQRDWDLALD
                     IESMPLQLLSNRIPRQLRLTGGRTSAEADLAKRDGAP"
     gene            complement(1981..>2571)
                     /locus_tag="VIW27_05105"
     CDS             complement(1981..>2571)
                     /locus_tag="VIW27_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY5739080.1"
                     /translation="DGDSNLLIPGVAWSRTWGQDFINVLDGLRFDLSLSGASESLASD
                     LDFAQAVGGIKFITSFDNRNRLIARGGAGATETKDFDLLPASLRFYAGGSQTVRGYKY
                     KSLGPTNDDGDVIGARFLLFGGLEFEHYFDDRWGVAVFIDAGNAIDNINEDLEQGAGF
                     GLRWKSPIGPVRVDLANAISDEDQPWRLHINIGPDL"
BASE COUNT          436 a          849 c          809 g          477 t
ORIGIN      
        1 ggggcgcccc gtcgcgtttc gccagatcgg cctcggcgct cgtcctgcct cctgtcaggc
       61 ggagttgtcg tggtattcga ttcgacaata gttgtaacgg catgctttcg atgtcgagcg
      121 cgaggtccca gtcccgttga tttccgctcc acgcactgca aacccggccc tggttatcgc
      181 ccagcaggca actccgggcc aggctcgagt actccgggga cagggtaatc gcggcgggcg
      241 ccgacagttt ccagttcgaa aattcctcgg tttcgaccgt caggccgacc agcatgccac
      301 gccaggcagc gccgtcgagg ctgccggcga gcgcgatatc cgtgcttccc aggatactga
      361 ccaggtgcgc gtcgatgcgc ttgggatcgg ccctgacggt aacgcgttcg aattcccgca
      421 gcccgatacg caggtcctcg gcatcgaggt cgatgtcgaa tcgctccgct tgcaagggct
      481 cgacgctcgc tttaccggta atcttgccga cgcggtagtc ggcgtattcc agtgcctcgg
      541 ccgcgaggct ggcttcgatc cgcggcgcgt cgcgcgtgcc gcgtaccatg ccctctagat
      601 cgatatcgcc cctggcgccg gggtagaatt cggccaggtc gtcgctgtgc aacgcccagc
      661 tcaggtccag tgtatcgtcc gcacggccgc tggcggtgag ccgggttccg ccgctgtcga
      721 tttcgagcgc ttcgatcgac aggccttgtc ttcgccactc gcccgatccg cgcgccgcga
      781 ccgggtagcc gcgcaattca ccgtcgacac gcacgatcct ggcgctggcc gtcaatgcat
      841 cggcctgcca ggcgccgcgg gtcgtaatgc tgccgttcag gcgtccgggc cattcactgt
      901 cgaggcccgc gggatcgatt tcgcgcagcg aaatctcggc ctcccaggcc ggcacgggcg
      961 cccaggcgat ttcgccgcgc gcgccgattt cgccatccag cgcatgcagg acgaagtctt
     1021 cgacccgcat gccgtcgaag cggcgttcgc cttcgagacc gcgcaggtcg aagtccgcgc
     1081 tgaccgcacc gagcctcggt agcacggttt tcaggtttcc ctcggcagcc caggattcca
     1141 gatcgccttg cagcattgcc gcgaggctgc cgtcaagctc cggcaggctt gcatccagta
     1201 gccgggtatc gaattgcgcg acgtcgatgc gcgatcgcca gcgcagctcg cccggcaggt
     1261 cctcgagttc gagcagctgc cgcatccgta atgcgccatc gagctgttgt tccagtcgcg
     1321 ttgcggcgag gtcaccgctg agcttgccgg tgccgctcag atcggcgccg gaggcgagca
     1381 cggttttcca ttcgaaatca acctggtggt cccaggcatc gccggttgcg atgtgaccgc
     1441 tcagaccggc gtcgagaaac tccgattgca gttgcaggtt gccgaattcg aatcgatcgc
     1501 catccgcgta cagattcccg cccaatcgct ccaggcgcca ggctgaatcg ccacgggcga
     1561 tctcgattgc ccgcaattcg agggcctcga ggcgcagttt caaaggcagt cggatttccg
     1621 gtgcagtgaa cggtcggggt tccgcctgcg ggtccggatt tgccgctggt aatgcaatgc
     1681 gcagcgagtc gattcgaaga ctgtcgattt cgagggtaaa cgcggcgagc gcccagggat
     1741 tccagtcgag ttcgacgcgc ccggccgcga gcgtcagttc accatcggcg aagcttgccg
     1801 cggacaattc gatgggaccg gccagtaccc cggaaaccga cgcagcctgg aggtctcccg
     1861 gcagccagga agcggtcgta cgccaggccc aacggagccc ggattcggta ttcatcagcc
     1921 agccggcgcc ggcggccgcc agtaccagcg ctacactcga ggcgatcagg attcggcgca
     1981 tcacaggtcg ggcccgatat tgatgtgcaa tcgccacggc tggtcttcgt cgctgatcgc
     2041 attggcgaga tcgacacgca ccggcccgat cggcgatttc cagcgcaggc cgaagcccgc
     2101 cccctgctcg agatcctcgt tgatgttatc gatcgcgtta ccggcgtcga tgaacacggc
     2161 gaccccccat cggtcgtcga agtaatgttc gaactcgagt ccgccaaaca acaggaacct
     2221 ggcgccgatg acatcgccgt cgtcgttggt cggccccagc gacttgtact tgtatccacg
     2281 cacggtctgc gaaccgccgg catagaatct cagcgatgcg ggcagcaggt cgaaatcctt
     2341 cgtctcggtg gcacctgcgc cgccgcgagc gatcagtcgg ttgcggttat cgaaggaagt
     2401 gatgaacttg atgccgccga cagcctgcgc aaagtcgagg tcggaagcca ggctctcgct
     2461 cgcgccggag aggctcaggt cgaagcgaag gccgtcgagc acgttgatga aatcctgacc
     2521 ccaggtgcgg ctccaggcta ctcccggaat caacagattg gagtcgccat c
//
LOCUS       DATCZV010000230         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_441836, whole genome shotgun sequence.
ACCESSION   DATCZV010000230 DATCZV010000000
VERSION     DATCZV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441836"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VIW27_05110"
     CDS             complement(<1..151)
                     /locus_tag="VIW27_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5739081.1"
                     /translation="MLRRSKIIATLGPATDDVKTLCKMIDAGLDVARLNFSHGNHDAQ
                     RQRLEM"
     gene            267..1649
                     /locus_tag="VIW27_05115"
     CDS             267..1649
                     /locus_tag="VIW27_05115"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEY5739082.1"
                     /translation="MLERRRFLQLIAAAAVPSARASATRSPVYGPFGSLRPDPQGILD
                     LPENFSYEVISRRGEEMDDGLLVPARHDGMAAFPGRNGETLLVCNHENPARLQDFGPF
                     GAALERLDRIDKSEIYDYGKGSSPGTGGTTTIAYDTRTRQRKRIHLSLAGTEINCAGG
                     PTPWGSWLSCEEIFRDPGSSFEWSAVVNREQKHGYIFEVHAADEGLADPVPLKAMGRF
                     EHEAAAVNPATGVVYMTEDRYRSLFYRFIPDVAGRLQEGGRLQALAVKGVPEFDTRNW
                     VGRERLQPGAWLETSWVDLEEPDVAENDLRFRGREKGATIFARGEGLCYADGAFFMTA
                     TIGGPSRLGQVFTYRPSAAEGTPGEERNPGRLALIAESTHESLLRHADNLTMSPWGDL
                     VVCEDTARHCGLIGLTPDGTQYPIADNAYTSSELAGVCFSPDGSTLFVNIQDRGLTVA
                     ITGPWPVTNG"
     gene            complement(1636..>2317)
                     /gene="pgk"
                     /locus_tag="VIW27_05120"
     CDS             complement(1636..>2317)
                     /gene="pgk"
                     /locus_tag="VIW27_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY5739083.1"
                     /translation="ELAALGQALEDPRRPMVAIVGGSKVSTKLDVLAALADIVDTLIV
                     GGGIANTFIAAAGYEVGKSLHEADMLATAKALANDRDDRAVIPLPVDVVVASEFSADA
                     PASTRAIDDVGDEELILDIGPQTAERFAEIVAGAGTVIWNGPLGVFEFDQFGAGTEKL
                     ARSIAASSAFSVAGGGDTLAAIDKYGVADDISYISTGGGAFLEFVEGKKLPAVAILEE
                     RALDTSRS"
BASE COUNT          453 a          711 c          701 g          452 t
ORIGIN      
        1 gcatttcgag tcgttgccgc tgtgcatcat gattgccgtg agaaaaattg agcctggcga
       61 cgtccagacc ggcatcgatc atcttgcaaa gcgttttcac gtcgtcagtt gccggtccga
      121 gcgtcgcgat tatctttgat ctgcgcagca tgggcgggat tattgcacag ctgaccaccg
      181 gtcgctgcgg gcattcccta tgtactcgat ccgtaacgag gttaagctac tatgccaaat
      241 ctgctgtttg accaaagaat gtgccaatgc tcgaacgccg ccgtttcctg caattgattg
      301 ccgccgccgc cgtgccgtcc gcccgggcgt cggcgaccag gtcgcccgtc tatggcccgt
      361 tcggaagtct gcggcccgat ccgcaaggca tcctcgacct gccagagaat ttcagctacg
      421 aggtaatttc ccggcgcgga gaggaaatgg acgatgggct cctggttcct gccaggcatg
      481 atggcatggc ggcattcccc gggcgaaatg gcgagaccct gctggtatgc aatcacgaaa
      541 atccggcaag gctgcaagac ttcggtccgt tcggcgcggc gctggagcgc ctcgacagga
      601 ttgataagtc ggagatttac gactatggca aaggcagtag ccccggcacc gggggaacta
      661 cgacgatcgc ctacgacacc aggacgcggc agcgcaagcg tatccatctg agtcttgccg
      721 gaaccgagat caattgtgcc ggcgggccta ccccctgggg cagctggctg tcttgcgaag
      781 aaatttttcg cgatcccggc tcgagtttcg agtggagcgc ggtcgtcaat cgcgagcaga
      841 aacacggcta catcttcgaa gtgcatgcgg cggacgaggg gctggcagat ccggtgcccc
      901 tgaaagccat gggtcgtttc gaacacgaag ccgccgcggt caatccggcg accggcgtcg
      961 tttacatgac cgaagatcgt taccgcagcc tcttttatcg ttttattccg gacgtggcgg
     1021 gccgacttca ggagggcggc aggctgcagg cgcttgcggt caagggcgtg ccggagttcg
     1081 acacgcgaaa ctgggttggc agagagaggc tgcaaccggg ggcctggctc gaaacgagct
     1141 gggtcgacct ggaagaaccg gacgttgccg aaaatgacct gcgatttcgc ggccgggaaa
     1201 aaggtgccac cattttcgcg cgcggcgaag gattgtgtta cgcggacggc gcgtttttca
     1261 tgaccgccac catcggcggc ccgtcgcgcc tcgggcaagt tttcacttat cggccgagcg
     1321 ctgccgaagg cacgcccggt gaagagcgaa atcccggacg actcgcgcta atcgcggaat
     1381 ccacccacga gagtctgctg cgacacgccg ataatttgac catgagtccc tggggcgacc
     1441 tggtcgtctg tgaagacaca gcacggcatt gtgggctaat tggtttgacg cctgacggta
     1501 cgcaataccc gatcgccgac aatgcctaca ccagttcaga gctggcaggc gtctgcttct
     1561 cgcccgatgg ctctacgcta ttcgtcaata tccaggaccg cggcttaacg gtagcgatca
     1621 ccggcccgtg gcccgtcacg aacggctagt atccagagcg cgctcctcga gaatcgcgac
     1681 tgccggtaac ttcttaccct cgacaaactc gaggaacgcg ccgccgcctg tcgagatgta
     1741 ggatatgtcg tcggcaacgc cgtatttgtc tatcgctgcc aaggtatcgc cgcctccggc
     1801 gaccgaaaac gccgaactcg ccgcaattga ccgcgccagt ttttccgttc cggcgccaaa
     1861 ctgatcgaat tcgaaaacac ctagcggccc gttccagata acggtgcccg cgccggcaac
     1921 gatctcggca aaccgttctg cagtttgcgg cccgatgtcg agtatcagtt cctcatcgcc
     1981 gacatcatcg atggccctgg ttgacgccgg tgcatccgcc gaaaactcgc ttgccaccac
     2041 gacatcgacc ggcaacggaa tcacggcgcg gtcatcgcgg tcgttggcaa gcgctttggc
     2101 cgtcgccagc atgtcggctt cgtgcagcga cttccccacc tcgtagccgg cggcggcgat
     2161 gaaggtgttc gcgatgccgc cgcccacaat cagggtatca acgatgtctg cgagcgcggc
     2221 cagtacgtcg agcttcgtcg agactttcga tccgccgacg atcgcgacca tcggtctgcg
     2281 cggatcttcg agcgcctggc caagcgccgc gagttcc
//
LOCUS       DATCZV010000231         7634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_177393, whole genome shotgun sequence.
ACCESSION   DATCZV010000231 DATCZV010000000
VERSION     DATCZV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177393"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..765)
                     /locus_tag="VIW27_05125"
                     /pseudo
     CDS             complement(347..765)
                     /locus_tag="VIW27_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017014462.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
     gene            1459..2238
                     /locus_tag="VIW27_05130"
     CDS             1459..2238
                     /locus_tag="VIW27_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY5739084.1"
                     /translation="MVELSERQVEITERLREQGFLAVETLAEEFRVTTQTIRRDLTTL
                     CDHGLARRRHGGIERLVDGGNLAYVSRQILARHAKQAIAHEVAQHIPDYASVAFSIGT
                     TLEVVATALLQHKGLRIFTNNLNVAMLARANPTFEVNIAGGRIRNGDNDVLGPDLENF
                     FSSYIFDFGIYGVAGVSERGTLLDFYDEEVRARRLIHDNSRENFLVLDHSKFGRTAHV
                     RGGQIGEASKVFCDARPPKPIVEALEKSGSQLVICGEDQNQ"
     gene            2235..3239
                     /locus_tag="VIW27_05135"
     CDS             2235..3239
                     /locus_tag="VIW27_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020027766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739085.1"
                     /translation="MTGITITNLVKEWGTTRAVDDVSFTVQEGTLTVLLGPSGCGKST
                     ILRMIAGLESVTSGSVDIGGTDVTRLDPAKRGVSMVFQSYALFPHLDVRENILFGLKV
                     RRVDPQEQQKRLMDAANMVGLEKLLDRKTAQLSGGQRQRVALARTIVAKQPVCLMDEP
                     LSNLDAKLRAEMRDEIHRLQRGLGLTMVYVTHDQTEAMSMADQVILLKDGRIVQIGTP
                     AELYDWPATDFAAQFLGTPPMNVLPAAELGGRVPLPGEGHYAGIRPENVVIADEGLEV
                     EVFAVDYMGAETVLRLRHGDNMIMARVDGRADATDGDQLHITWDASDVHLFDQSGSRL
                     "
     gene            3361..4641
                     /locus_tag="VIW27_05140"
     CDS             3361..4641
                     /locus_tag="VIW27_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739086.1"
                     /translation="MFTIIKARLTRILLAAVFAIGTTAAQAATELTMYYPVAVGGPLT
                     QVVDGIIADFMKENPDIKVNAIYAGNYNDARIKALAALKSGEPAQLSVMFSIDIYELI
                     EQDAIVAFDDIVTTAEDKKWLDSFYPSLMENGRTKGKTWGIPFQRSTIVMYYNKDAFR
                     AAGLDPEKPPATWDELVAAGKKLTKSDGSQWGMMIPSTGYPYWMFGALTMQNDQVLMS
                     GAGNETYFDAPATVEALQFWKDLGAKHKVMPDGTIEWGTLRQNFLEGKTAIMWHSTGN
                     LTTVKNNASFDFGIAMLPANERRGTPTGGGNFYIFKDTSPEERTAAMKLIKFMTAPER
                     SAEWSIKTGYMGVSPAAYQTQALKDYVKGFPYAAVARDQLEYATAELSTYETGRIRKA
                     LDDAIQAALTDAKTPEAALKEAQATAKRLLRVYQ"
     gene            4726..5589
                     /locus_tag="VIW27_05145"
     CDS             4726..5589
                     /locus_tag="VIW27_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5739087.1"
                     /translation="MRHVYGWLLLTPAAILLITFTHYPTVATFYESLFSKGTLTRPSK
                     FIGIDNYQFMTEDPIFWKVLVNNLWFALGTIPASIALAIAMALLVNKALPGRSFLRLA
                     YFTPTILPMIAVANIWLFFYSPDIGLIDQVLGIFGFSGHNWLGDPGTVLGAIIVMTIW
                     KEAGFFMIFYLAALQNLPPELEEAAKLEGASRWYYFRRVVFPLLMPTTLFVLINAVLN
                     SFKLVDHLFVLTKGGPDNASNLLLYYIYENAFSYFDTSYAATLTVVLLTMLALMALGQ
                     FFILDKRVHYR"
     gene            5586..6422
                     /locus_tag="VIW27_05150"
     CDS             5586..6422
                     /locus_tag="VIW27_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5739088.1"
                     /translation="MIQNEATDRKWTFADVVELAGAWLLAILWVSPLAYAFWAAFHAS
                     EYAIHFDLFAPLTLDNFRQALDQAPFLRYGFNTFVLVSMLLVGQFVLCTLAAYAFARF
                     EFAGSNIAFALVLVQLMITPEILIVENYVTMANLGLVDTITGIGLPYMASAFGIFLLR
                     QTFKSTPKELEEAARLEGCGMLGILWRVYIPLARPTYLAYGLVSISHHWNNFLWPLVI
                     TNSVETRPLTVGLGIFGAPESGVDWSIVSAATLLSVAPLLIAFLLFQRQFIQSFMHSG
                     IK"
     gene            6419..7285
                     /locus_tag="VIW27_05155"
     CDS             6419..7285
                     /locus_tag="VIW27_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5739089.1"
                     /translation="MTSVLSWNIQNGKGCDGKISLERIAAVIGAMGTPDIICLQEVSR
                     GLVLEEGSAAPDQVAELQQLFPDYEIAFGVAFEARTPGKSERWQYGNAILTKMPLLMM
                     LAHPLPRPGVENIRHMTRQATEVVVEDDSGPLRIVNTHLEFHSAIHRMAQVDRLRDLQ
                     REAIEETTMPPLTSASGPYQQVARPVDAIYCGDFNMAPGSEEYRRLLAPLAGEAEPFI
                     DAWLQLYPDREHDPTCGIHDHEQWPEGPHCRDFFFAAGRCTACLRALRVDAKTDASDH
                     QPLMLELGGLSI"
BASE COUNT         1476 a         2443 c         2302 g         1413 t
ORIGIN      
        1 acatcggcaa taccctgccg cccatcctcg agcgactgga gatcatcgcc gggcaatggc
       61 gcagcaacac cacgcagttc gaggcattac acccgaagcg gtttaatcga gtcgtacctg
      121 cgctcgatac gggttaaccc ggaacactct actctcccaa tttcgaccgg tgaacagcgc
      181 ggacgggcga gtgtggctgg agatcacgct aactgcatga caatggctgt tgttgagtgc
      241 tggatgcgga tgaagggtgg atttgagggg ttgggttggg atcgcgcgtg gtgggcgaaa
      301 ttttcttgtt agagtgtgtg tccctattca cctgtctatt cacccgggtg gaaacgccgg
      361 tgatcggaat atcgccgcag gccgagacca ccgcgcggtg cgtgccgtcg ccgccgagca
      421 cgatgatcgc cgcgaccccg gcggcgcgca tacggcgcgc ggcctcgacg ctgtcgccgg
      481 tcgaggtgct gaccggcatg tcgaggtatt cgacgcgcgg aaaacgcgcc tcaccgagct
      541 tgccggagcg ctccaggccg ccaccacgtg ctggcgaatg ccggaatact cgggcatcac
      601 taaaactcga tcgatgccga ccgcgccgag cccggcgagc aggtgcagca cgatgttggc
      661 acgatcggtg atctgcaggt tgcccgcgta ggcgacgatg cggcgaatgt cgcgcgccga
      721 caccgggttg gcgatgatgc cgacgacggg cctgtccgcg ctcatctgcg atccctgagc
      781 gcgcaacggc acgagatgtc gaaatcgata aacgcgaacc gccgtggcgc agtaccaatg
      841 gcgctcccgg ccaggcttgc gggcataatc gcgcgggcag gggtcatgtc gttattcgtt
      901 gtcctcccgg tgctttccgg tgttccacac cggaatccag cctcccgaat ccccgccgat
      961 gaaatcagcc gtgccgcgta aagccgccgg cgctcctggt ggcgggaatg ataattacta
     1021 ctcagctcgt ccggcggcgt cgactttgga atgggatcgg ggatccattg cactcgatga
     1081 cgggaccgaa tgcgcctgtt cgagtagtcc cggtcgatcg ccacccgctt gcccgcagtg
     1141 gtcccgcccg cagcaggcgg cgttttcgaa ctcatcccgc gcatgcagga ttcggtaatg
     1201 gcggcaagcc tggagattgc cgcttgcgcg gccatgactc cggcgcgggc agggcggcaa
     1261 taacaccgcg tcgatcctgc cgagacaggt gctaatgaat acgcgaatcg ccgtcgcagg
     1321 ttggcgccga tgatcgcatt ttgagttgtt atgaaactga tctgtaagga attattttca
     1381 tatgaacatc aaatgatgcg tgttagaaaa taatctgcta tcgtatgacg ctcaaaaagg
     1441 gggaggcacc ggttatcaat ggtggagctt tcggagcgcc aggttgaaat cacggagcgt
     1501 ctgcgcgagc agggatttct cgcggtcgag acgctggccg aagaatttcg ggttaccacg
     1561 caaaccattc gccgtgatct gacgacgctc tgcgaccacg gcctggcgcg gcgccgccac
     1621 ggtggtatcg agcgtctcgt cgatggcggc aatctcgctt acgtatcgcg ccagatcctc
     1681 gcccgccacg ccaagcaggc catcgcgcac gaagtagcac agcatattcc cgactatgcc
     1741 tcggtcgcgt tcagtatcgg cactaccctg gaagtcgtcg ccacggcttt gctgcagcac
     1801 aagggcctgc gcatcttcac caacaatctc aacgtcgcca tgctggcccg cgccaacccg
     1861 accttcgagg tcaatatcgc gggcggccgg attcgcaacg gcgacaacga cgtcctcggg
     1921 ccggacctcg aaaacttctt ttcgtcctat atcttcgatt tcggcatcta cggcgtcgcc
     1981 ggcgtcagcg agcgcggcac cctgctggac ttctatgacg aggaagtccg cgcgcggcgg
     2041 ctgatccacg acaacagccg cgagaacttc ctggtgctcg accacagcaa gttcggtcgc
     2101 acggcccacg tcaggggcgg ccagatcggc gaggccagca aggttttctg cgacgcacga
     2161 ccgccgaaac ccatcgtcga agccctggaa aaatcaggta gccaactggt gatctgtggt
     2221 gaggaccaaa accaatgaca ggcatcacaa tcacgaatct cgtcaaggaa tggggaacca
     2281 cgcgcgcggt cgacgacgtc tcgttcaccg tgcaggaagg cacgctgacg gtcctgctcg
     2341 gcccctcggg ctgcggcaag tcgaccatcc tgcggatgat cgccgggctt gagtcggtca
     2401 catccggcag cgtcgacatc ggcggcaccg acgtcacccg gctcgatccg gcgaagcgag
     2461 gcgtctccat ggtgttccag tcctacgcgc tgttcccgca cctcgatgtg cgcgaaaaca
     2521 ttctcttcgg attgaaggtg cgccgcgtcg acccgcagga gcagcagaag cggctcatgg
     2581 atgcggcgaa catggtcggc ctcgaaaaac tgctcgatcg caagaccgcg cagctgtccg
     2641 gcggccagcg ccagcgcgtc gcgctcgcgc gcaccatcgt cgccaagcag ccggtgtgcc
     2701 tgatggacga accgttgtcc aacctcgacg ccaagctacg cgccgaaatg cgcgacgaga
     2761 tccatcgcct gcagcgcggc ctggggctca ccatggttta cgtcacccac gaccagaccg
     2821 aggcgatgtc gatggccgac caggtgatcc tgctcaagga cggccgcatc gtgcagatcg
     2881 gcacgccggc cgaactctac gattggccgg cgacagactt cgctgcgcag ttcctcggca
     2941 cgccgccgat gaacgtgctg cccgccgccg agctcggcgg ccgcgtgccg ctgccgggcg
     3001 agggccacta cgccggcatt cggccggaaa atgtggtgat cgccgacgag ggcctcgagg
     3061 tcgaggtttt cgcggtcgat tacatgggcg ccgagaccgt gctgcgcctg cgccacggcg
     3121 acaatatgat catggcgcgc gtcgacggcc gcgccgacgc gaccgacggc gaccagctgc
     3181 acatcacctg ggacgccagc gacgtgcacc tgttcgatca gtcgggaagc cgattgtgat
     3241 gccgcggacg gttaaccgtg tcatcgccgg ccggcgtgag cgcgccggca ggattatttt
     3301 tgcgcagcgg atgcgcacca gttctttacc tgataaacct caaaaaaagg aggaagcacc
     3361 atgtttacga taatcaaggc gaggctgacc cgcatcctgc tggctgccgt tttcgccatc
     3421 ggcacaaccg ccgcgcaggc cgccaccgag ctgaccatgt attacccggt cgcggttggt
     3481 gggccgctga cccaggtggt cgacggcatc atcgccgact tcatgaagga aaacccggac
     3541 atcaaggtca acgcgatcta cgccggcaac tacaacgacg cgcgcatcaa ggcgctcgcg
     3601 gcgctgaaga gcggcgagcc ggcgcagctg tcggtgatgt tttcgatcga catctacgaa
     3661 ctcatcgagc aggacgcgat cgtcgccttc gacgatatcg tgaccaccgc cgaggacaag
     3721 aagtggctgg attcgttcta tccgtcgctg atggagaacg gccgcaccaa aggcaagacc
     3781 tggggcattc cgttccagcg ctcgaccatc gtcatgtact acaacaagga cgccttccgc
     3841 gccgccggcc tcgaccccga gaaaccgccg gcgacctggg acgaactggt cgccgcgggc
     3901 aagaagctga ccaaatccga cggctcgcag tggggcatga tgattccgtc gaccggttac
     3961 ccctactgga tgttcggcgc gctgaccatg cagaacgacc aggtgctgat gagcggcgcc
     4021 ggtaacgaga cttacttcga cgcgccggcc acggttgagg cgctgcagtt ctggaaggac
     4081 ctcggcgcga agcacaaggt catgccggat ggcaccatcg aatggggcac gctgcggcag
     4141 aacttcctcg agggcaagac cgcgatcatg tggcactcca ccggtaacct gacgactgtc
     4201 aagaataacg cgagcttcga tttcggcatc gccatgctgc ctgccaacga gcgccgtggc
     4261 acgccgaccg gcggcggtaa tttttatatc ttcaaggaca ccagccccga ggagcgcacc
     4321 gcggcaatga agctgatcaa gttcatgacc gcgcccgagc gctccgccga gtggtcgatc
     4381 aagaccggtt atatgggcgt gagcccggcg gcttaccaga cccaggcgtt gaaggactac
     4441 gtcaagggct tcccgtatgc cgcggtcgca cgcgaccagc tggaatacgc gacggctgag
     4501 ctgtctacct acgagaccgg ccgcatccgc aaggcgctcg acgatgccat ccaggccgcg
     4561 ctgaccgatg cgaaaacacc cgaagctgcg ctcaaggaag cgcaggccac ggcgaagcgc
     4621 ctgttgaggg tttaccagta gagcgattcc cggtggcggc gtcgagcggc gccgccaccg
     4681 ggatcgaacc aggggaaggg gaatcgagtg caatcgacca acgcgatgcg acacgtttac
     4741 ggctggctgc tgctgacgcc ggccgcgatc ctgctgatca catttaccca ttaccccacg
     4801 gtcgcgacct tttacgagag cctgttttcc aagggcacct tgacgcggcc atcgaaattc
     4861 atcggcatcg acaattacca gttcatgacc gaggacccga tcttctggaa ggtgctggtc
     4921 aacaacctgt ggttcgcgct gggcacgatc ccggcgtcga tcgcgctggc gatcgccatg
     4981 gcgctgctgg tcaacaaggc gctgcccggg cgctcgttcc tgcggctcgc ctatttcacg
     5041 ccgaccatcc tgccgatgat cgcggtcgcc aacatctggc tgtttttcta ttcgccggac
     5101 atcggcctga tcgaccaggt gctcggcatc ttcggcttct ccgggcacaa ctggctcggc
     5161 gaccccggca ccgtgctcgg cgcgatcatc gtcatgacga tctggaaaga ggccgggttt
     5221 ttcatgatct tttacctcgc cgcgctgcaa aacctgccgc ccgaactcga agaggcggcg
     5281 aagctcgagg gcgcatcgcg ctggtattac ttccgccgcg tcgtgtttcc gctgctgatg
     5341 ccgaccacgc tgttcgtgct gatcaatgcg gtgctgaact cgttcaagct ggtcgaccac
     5401 ctgttcgtgc tcaccaaggg cgggccggac aacgccagta acctgctgct ctactacatc
     5461 tacgaaaacg cgttttccta cttcgatacc agttacgcgg cgacgctgac cgtggtgctg
     5521 ctgacgatgc tggcgctgat ggcactgggg cagttcttca ttctcgacaa gcgggttcac
     5581 taccgatgat ccagaacgaa gcaaccgaca ggaagtggac cttcgccgac gtggtggagc
     5641 tcgccggcgc gtggctgctc gccatcctgt gggtctcacc gctggcgtat gcgttctggg
     5701 cggcgtttca cgccagcgaa tacgcgatcc atttcgattt gtttgcgccg ctgacgctcg
     5761 acaacttccg ccaggcgctc gaccaggcgc cgttcctgcg ctacggcttc aacaccttcg
     5821 tgctggtcag catgctactg gtgggccagt tcgtgctgtg cactctcgcg gcttacgcgt
     5881 ttgcgcgctt cgaattcgcc ggcagcaata tcgccttcgc gctggtgctg gtgcagctga
     5941 tgatcacgcc ggaaatcctc atcgtcgaga actacgtgac catggccaac ctcggcctgg
     6001 tcgacaccat caccggtatc ggcctgccgt acatggcgag cgcgttcggc attttcctgt
     6061 tgcgccagac cttcaagtcg acgccgaagg aactcgagga ggccgcgcgg ctcgagggct
     6121 gcggcatgct cggcatcctc tggcgggtct acattccgct cgcgcggccg acctatctcg
     6181 cttacgggct ggtgtcgatc agccaccact ggaacaactt cctgtggccg ctggtcatca
     6241 ccaactcggt cgaaactcgg ccgctgaccg tcggcctcgg catctttggc gcgccggaat
     6301 cgggcgtcga ctggtcgatc gtctcggccg ccacgctgct gtcggtggcg ccgctgctga
     6361 tcgcctttct gctgtttcag cgccagttta tccagtcctt catgcactcc ggcattaaat
     6421 gacgagcgtc ctgtcgtgga acatccagaa cggtaagggg tgcgacggca aaatctcgct
     6481 cgagcgcatc gccgcggtga tcggcgcaat gggcacgccc gacatcatct gcctgcagga
     6541 agtctcgcgt ggcctggtgc tcgaggaagg cagtgccgcg cccgaccagg tcgccgagct
     6601 ccagcaactg ttccccgatt acgaaatcgc cttcggcgtc gcattcgaag cccgcacgcc
     6661 gggcaaaagc gagcgctggc aatacggcaa tgcgatcctg accaaaatgc cgctgttgat
     6721 gatgctcgcg cacccgctgc cgcgccccgg ggtcgagaac atacgccaca tgacccgcca
     6781 ggccaccgag gttgtggtcg aggacgactc gggcccgctg cgcatcgtca acacccacct
     6841 cgagtttcac agcgcgattc accgcatggc ccaggtcgac cgcctgcgag acctgcagcg
     6901 cgaagccatc gaagaaacca ccatgccgcc gctgaccagc gccagcggcc cctaccagca
     6961 agtcgcaaga ccggtcgacg ccatctactg cggcgatttc aacatggcac ccggctcgga
     7021 agaataccgg cgcctgctgg cgccactggc cggggaggca gaacctttta tcgatgcctg
     7081 gctgcagcta taccccgacc gcgaacacga tcccacatgc ggcatccacg accacgagca
     7141 atggcccgag ggcccgcatt gccgcgattt cttcttcgcc gccggtcgct gtacagcttg
     7201 tcttcgtgct ttgagagtcg acgccaaaac cgacgcctcg gaccaccagc cactaatgct
     7261 ggagttgggc ggcctctcca tctaacacga ctcatcgcca ccgactcgga agtcttcatc
     7321 gcaacccctc cgtcatcccc gcgaaagcgg ggatcttgct atgtcaagat gatcttgaga
     7381 ctcctttcca acgcaagcgg ctaattaatc gccgaacgct acgatctggt cctgcccagc
     7441 actgggagcg gaagaagctg gaacggttat gccgttacat taccaaagcc gtcataccaa
     7501 acgttaaata tttttgggtc atagctaaaa ctcccataat tttggctctg acccaaaatt
     7561 atgggatgtc ggctacgggc acagacctga cacctgatca ctaggcttgc gtggcggtct
     7621 tgggtcggtt cgtg
//
LOCUS       DATCZV010000232         7796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_70722, whole genome shotgun sequence.
ACCESSION   DATCZV010000232 DATCZV010000000
VERSION     DATCZV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70722"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1128)
                     /locus_tag="VIW27_05160"
     CDS             complement(184..1128)
                     /locus_tag="VIW27_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739090.1"
                     /translation="MTYEMTSRFSPQTLAKFASLYAGLAYGLYWIPLRALEQAGLHDV
                     LPALMFNLVPMVLILPLIVWRWPNIARAPMHFHLTGVVVGLALVGYTNAFLYADVVRV
                     LILFYLTPIWGFILARIFLGDLITPVRWISILLGIGGMLTIFDVGTGIPLPESVGDWI
                     ALAAGVLWAIASLMMLMGKESPIDYALWFFLWNGIAAIAVCAIFFAGDLTALPPPAVF
                     IDVLPWLVPLALVLIIPAGFAVIYGPTQLNPGIAGVLFMVEIGVGTITASILTDEPFG
                     WRELSGVLMITAAALIEPVRDLIRHRYGRDQSDFLDFS"
     gene            1523..3334
                     /locus_tag="VIW27_05165"
     CDS             1523..3334
                     /locus_tag="VIW27_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5739091.1"
                     /translation="MTKGIKPQYLYGTLLAATLTLTFWATWSFSWHQGLAELQQGNQQ
                     YLEQFSGHLDSRLSQFRFLPRLVAKNELLSELLISPDSLPNVQAVNRFLEEVNDITGA
                     SDTYLMNTMGLTIAASNWRKQLTFVGQNFSFRPYFAQAMQGQVGHYFALGTTSKERGY
                     YFSYPVRRAAGIVGVLVIKMDLQNIEQYWSSQDTRFLATDDDGVIFITTTPEWLYRSI
                     RPLNESDIERIRKTRRYADAEVAALTYRPRDEVADDSSIVDIGMPGQAPTRYLASVQQ
                     MPVAGWSVHVLTPLSELTGESLVLALAAVLLMISMLSIALLIRWRIRRQQERERIYRE
                     AQKELEQKVLERTSDLQNEIQLHKATEQTLRATQDELIQAAKLALVGEMSASISHELN
                     NPLAAIRSYADNARRLLAKREEGKVDENLERISGLTERMAKISTQLKFFARKSSGRLE
                     PANLGPVIQTAIEIAGPQYRKKPIRIDTQGVDSGIQARVDTIQLEQVLINLISNAIHA
                     IGDQGDGEIRIATEQADGSVLIHVDDSGPGIDAKDLSRIFEPFFTTRESGLGLGLSIS
                     ARIVDGMNGKLTARNLNGGGARFTVALPGNEQFDEHA"
     gene            3321..4670
                     /locus_tag="VIW27_05170"
     CDS             3321..4670
                     /locus_tag="VIW27_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5739092.1"
                     /translation="MSTPDVYFIDDELDLRLANEQTLQLAGFSVALFEKAEDALPQLD
                     AQTGGVVVSDIRLPGINGLGLLQALQQVDSALPVILITGHGDISMAVDAMQKGAYDFI
                     EKPFSSERLVDTVRRALEKRRLTLENRDLRIELDEKDALGPRLIGKTAAMKALRRSIR
                     QLADTSADILLVGETGTGKELAARSLHEHSSRRERNFVAINCGAIPENLIESELFGHE
                     KGAFTGAEARRIGKFEYADRGTVFLDEIESMPLAAQVRLLRVLQERRLERLGSNEGID
                     LDIRIVAASKVDLKQAAEHGEFREDLFYRLNVVTLALPPLRERREDIPLLFQHFLLVA
                     AARYGKEAPALPADFSRSLMVYNWPGNVRELRNAAERFVLLGDAPGLQPDAADGGSPG
                     VPMTLPEHVEAFERALIEQALAESGGVIKETMERLGLPRKTLYDKMQKYGLDKRRYR"
     gene            4913..5884
                     /locus_tag="VIW27_05175"
     CDS             4913..5884
                     /locus_tag="VIW27_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY5739093.1"
                     /translation="MNIKRRSFLVAGSALLAAGMMMPLGAAAKERVVFSGGPAGGTFQ
                     VVANAIQVYDPIKESDTFRVRAQSSAGSVENLRKVNSGKAQMGVVYSGHVYLGRNGKM
                     KNDTKKYEDVMAVAWLYGAPAQLVVRKDSGIKSTKDLVGKKVGVGNAGSGAFANCELF
                     FTHMGVWDKIERNAMGYNDAAQAFGNNQLDAFWLFTAFPSGAVIMAAQTNDIELIDLG
                     KDAKDSGFFDQYPYFGQLSVPAGTYRGVDTETPSFQDSALWVANSKVSEQTVYEMLSA
                     IYTDEGLAHMKAQKKTFKNMSLESGTQGVVTPWHPGAVKFWKEKGMM"
     gene            5996..7735
                     /locus_tag="VIW27_05180"
     CDS             5996..7735
                     /locus_tag="VIW27_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEY5739094.1"
                     /translation="MYEKLHKVERLMFDAMGIGLVLFYSYSAVLQPAATQYHRGIYVV
                     ITYVLVLMLYRTPGVAGRVWDYFLMLVSVVALGYWIVNFEAINYRAGAETELDQWVAM
                     AGVLVGIEIARRVVGTVFVVIGTVMLLYGVFGDSEMIPEVLQHAGDTFPLLCTSIFYK
                     SDGVFGIMANVLATYIILFVIFGAFLEKSGAQRFFIDFPLAAVGHKIGGPGKVSVIAS
                     GLFGSISGSAIANTVSTGAFTIPMMKKAGFKPHVAGGIEPAASIGGMFMPPIMGAGGF
                     IMAELTGLPYSQIMLVAIFPALMYFFSVFMMVHYEAKKENIVGEKSKRSAKEILRKEW
                     FYILPLVAITILMLMGYSPGYSAILGIVTCIAVSWFRKDTRIDLKGFVDASRSGAETS
                     LKIGATVGVIGIIIGVLTYSGLILTFADIVIDLAEGRLWLTILLVALASLVLGMGVPV
                     TAAYLITAVVAVPALTHLGVNELAAHMIVYWLSQDSNITPPVCIAAFAGATIAKASMW
                     ATAWVAFKFAKFLYLAPFIFGYVPAFSLDGSAMDIAITFVLVFFGTWAYSWFLSGIWL
                     KRKPKAAAAGEQN"
BASE COUNT         1550 a         2375 c         2421 g         1450 t
ORIGIN      
        1 gtattccagt gtgcgaaacg caatccgatc gaggcgcatt gatgcggcac atgtactgac
       61 tgttcaatca tccattagcg gtttgaatgt ctgccatccg cgctgatgcg ctggcaaact
      121 cgcacttaat ttctactcgc ctgtcgctgc cgcgcgtgcc ggaatggttc gagcgcccga
      181 aggtcacgaa aaatcgagga aatcggattg gtcgcgaccg tagcggtgcc ggatcagatc
      241 gcgaaccggc tcgatcaggg ccgcggcagt gatcatcagc acgccactga gctcgcgcca
      301 gccgaagggc tcgtcggtca ggatgctcgc ggtgatcgtg ccgacgccga tttcaaccat
      361 gaacaggaca ccggcgatcc ccgggttcaa ctgggtcgga ccatagatca ccgcgaagcc
      421 ggccggaatg atcagcacca gcgccaacgg caccagccag ggcagcacgt cgatgaacac
      481 cgccggcggc ggcagcgcgg taagatctcc ggcgaagaaa atagcgcaga cagcgatcgc
      541 cgcgattccg ttccagagga aaaaccacag cgcgtagtca attggacttt ccttgcccat
      601 cagcatcatc aggctggcga tagcccacag caccccggcg gcgagcgcga tccagtcacc
      661 aacactctcg ggcaacggaa tgccggtgcc gacatcgaat atcgtcaaca tgccgccgat
      721 gccgagcagg atcgagatcc agcgaaccgg ggtgatcagg tcgccaagaa atatgcgggc
      781 aaggataaag ccccagatcg gggtcaggta aaacaggatc aggacccgca ccacgtcggc
      841 atacagaaag gcgttggtat agccgaccag cgcgagcccg acgacgacac cggtgaggtg
      901 aaaatgcatt ggcgcgcggg cgatattcgg ccagcgccac acgatcagcg gcaggatcag
      961 caccatcggc accaggttga acatcagtgc cggcaatacg tcgtgcaatc cggcctgctc
     1021 gagcgcgcgc agcggaatcc agtaaaggcc gtaggcaagt cccgcgtaga ggcttgcgaa
     1081 cttggcaagc gtctgcggag aaaaacggct ggtcatctcg taggtcatta tttgcagctg
     1141 agtatatcca taattcgaaa ccgaacgaca accctgttgc cactgcagcg ctccgcgacc
     1201 ccgcgggcgc gcgccccgat ccaggccgca gccgtaaccc gccggcctcg aatagcgatc
     1261 cgcccgcggg cctgcgcgat ggtacgcggc gcgcgtgacg atcgccatat ttttccacgg
     1321 aggttcgagg agcggtcgtt tgcggccggc gcggttgccg ccggccgggg atcggcgctc
     1381 gcgcggcaat gaccgcggga ggtgtgatac tcgcacgggc ggcccgcgcg gacttttatc
     1441 tcgattgcta agcgccgcga ttcttctgat aatgggtaca ggaccacgat tggtcaaatc
     1501 cacccggcag ttcgcagcgc tcatgacgaa aggcatcaag cctcagtatc tttacggcac
     1561 cttgctggcc gcgactctga ccctgacctt ctgggctacc tggtcgttca gctggcacca
     1621 gggcctcgcg gaactgcagc agggcaatca gcagtacctc gagcagttct ccggtcatct
     1681 cgattcccgc ctgtcgcagt ttcgcttcct gccgcggctg gtggcgaaga acgaactgct
     1741 gtccgagttg ctgataagcc ccgacagtct gccgaacgtg caggcggtca accgctttct
     1801 cgaggaggtc aacgatatta ccggtgcctc ggatacctac ctgatgaata ccatggggct
     1861 gaccatcgcc gccagcaact ggcgcaagca gctgaccttc gtcggccaga acttcagctt
     1921 tcggccttat ttcgcgcagg ccatgcaggg gcaggtgggt cattatttcg cgctcggcac
     1981 gacctcgaag gagcgcggct attacttttc ctacccggtg cgccgcgcgg ccgggatcgt
     2041 cggtgtgctg gtcatcaaga tggacctgca gaacatcgag caatactggt cgagccagga
     2101 tacccgcttc ctggccaccg acgatgacgg ggtgatcttc attacgacga cacccgaatg
     2161 gctgtatcgc agtattcggc cgttgaacga aagcgatatc gagcgcatca ggaaaacgcg
     2221 gcgctacgcc gacgccgagg tcgctgcgct gacctatcgc ccgcgcgacg aggttgccga
     2281 cgatagctcg atcgtcgaca tcgggatgcc cggccaggcg ccgacgcgct acctcgcgag
     2341 cgttcagcaa atgccggtgg ccggctggtc cgtgcacgtg ctgacaccgt tgtccgagct
     2401 gaccggcgaa agcctggtgc tggccctggc cgcggtgcta ctgatgatct cgatgctgtc
     2461 gatcgcgctg ctgataagat ggcggatccg ccggcagcag gaacgcgaac ggatctaccg
     2521 tgaggcgcaa aaagagctcg aacagaaggt gctcgagcgc accagcgatc tgcaaaacga
     2581 aattcagctg cacaaggcaa ccgagcaaac cctgcgcgcg acccaggacg aattgatcca
     2641 ggcggccaaa ctggccctgg tcggtgaaat gtcggccagc atcagtcacg aattgaacaa
     2701 cccgctggcc gcgatccgta gctatgccga caatgcgcgc cggctgctgg cgaaacgcga
     2761 ggagggcaag gtcgacgaaa atctcgagcg gatttcgggg ctgaccgagc gcatggcgaa
     2821 gatcagcacc cagctcaagt tcttcgcgcg caaatcgagc ggccggctgg agccggcaaa
     2881 cctgggcccg gtgatccaga ccgcgatcga gatcgccggc ccgcagtacc gcaagaaacc
     2941 cattcgtatc gatacccagg gtgtcgactc gggcatccag gcccgcgtgg atacgatcca
     3001 gctggaacag gtgctgatca acctgatcag caatgccatc catgccatcg gcgaccaggg
     3061 tgacggcgag atccgcatcg ctaccgagca ggccgatggc tcggtgctga ttcacgtcga
     3121 cgattccggc ccgggtatcg acgccaaaga cctgtcgcgc atattcgaac cctttttcac
     3181 cacccgggag tccggcctcg gcctcggtct ttccatttcg gcgcgcatcg tcgatggtat
     3241 gaacggtaag ctgaccgcgc gcaacttgaa cggcggcggc gcccggttta ccgtcgcgct
     3301 gccggggaac gaacaattcg atgagcacgc ctgacgttta ctttatcgac gacgagctgg
     3361 acctgcgtct ggccaacgag caaaccctgc agctggccgg cttcagcgtg gcactgttcg
     3421 agaaggccga ggacgcgctg ccgcagctcg acgcgcagac cggcggtgtg gtggtcagcg
     3481 atattcgcct gccgggcatc aacgggctgg gtttgctgca ggccctgcaa caggtcgaca
     3541 gcgcgctgcc ggtcatcctg attaccggcc acggcgatat ctcgatggcg gtcgacgcga
     3601 tgcagaaggg cgcctacgat ttcatcgaaa agccgttttc ttcggagcgc ctggtggaca
     3661 cggtcaggcg ggccctggaa aagcggcgcc tgactctgga gaaccgcgat ctcaggatcg
     3721 aactcgacga gaaggacgcg ctcggaccgc gactgatcgg caagacggcg gcgatgaaag
     3781 cgctgcggcg ctcgatcagg cagctcgccg atacctcggc ggatatcctg ttggtcggcg
     3841 agaccggcac cggcaaggaa ctggccgcac gctcgctgca cgagcacagc tcgcgccgcg
     3901 agcgcaattt cgtcgccatc aactgcggcg cgattccgga aaacctgatc gagagcgaat
     3961 tgttcggcca cgagaaggga gcctttaccg gcgccgaggc ccggcgtatc ggcaagttcg
     4021 agtacgccga ccgaggcacg gtgtttctcg acgaaatcga atcgatgccg ctggcggcgc
     4081 aagtcaggct gctgcgggtg ttgcaggagc gtcgcctgga gcgcctcggt tcgaacgagg
     4141 gcatcgacct cgacattcgt atcgtcgccg ccagcaaggt ggacctgaag caagcggcag
     4201 agcacggcga gtttcgcgaa gaccttttct accggctgaa cgtggtcacg ctggcattgc
     4261 cgccgctgag ggaacgccgc gaggatattc cgctgctgtt ccagcatttc ctgctggtcg
     4321 ccgccgcccg ctacggcaag gaggcgcccg cgttgccggc ggacttcagc cgcagcctga
     4381 tggtttacaa ctggcccggc aatgtccgcg agctgcgcaa cgcggccgag cgtttcgtgc
     4441 tgctgggcga cgcacccggg ctgcagcctg acgctgctga cggcggttcg cccggtgtgc
     4501 cgatgacctt gccggagcat gtcgaagcct tcgagcgggc actgatcgag caggcgctcg
     4561 ccgagtccgg cggcgtcatc aaggagacca tggagaggct cgggctgccg cgcaagaccc
     4621 tgtacgacaa gatgcagaag tacgggctcg acaagcggcg ctaccggtaa tccgccaccc
     4681 cgacactcgc caatgggtcg attaccccca cgcgcaagat acccgaacgg gtggaaatcc
     4741 acccctgacc cgctcgacag ccacttcgcg actcgcgcaa taactaccta tttatcaatg
     4801 gcttgaatca atatttcgag gcctggcacg gattggcccg cgaattgcaa cctgactctc
     4861 attgccttta tcgacgggca ttttttcaat acacaaatca agggggtttc tcatgaacat
     4921 caaacgcagg tcatttctgg tcgccggttc ggcattgctc gccgccggca tgatgatgcc
     4981 gcttggcgcc gcggccaagg aaagagtggt tttcagcggc ggccccgctg gcggtacctt
     5041 ccaggtagtc gccaacgcga tccaggttta cgacccgatc aaggaatcgg atacgtttcg
     5101 cgtgcgcgcg caatcctccg ccggctcggt ggagaatctg cgcaaggtca attccggcaa
     5161 ggcacagatg ggcgtggtct attccggcca tgtctacctc ggccgcaacg gcaagatgaa
     5221 gaacgacacc aagaaatacg aagacgtgat ggcggttgcc tggctgtacg gcgcgcccgc
     5281 gcagctggtg gtacgcaagg attccgggat caagagcacc aaggacctgg tcggcaagaa
     5341 ggtcggcgtc ggcaatgccg gttccggcgc cttcgccaac tgcgagctgt tcttcacgca
     5401 catgggcgtg tgggacaaga tagagcgcaa cgcgatgggt tataacgatg ccgcgcaggc
     5461 cttcggcaac aaccagctgg acgccttctg gctgttcacc gcctttccga gcggcgcggt
     5521 gatcatggcc gcgcaaacca acgatatcga actcatcgat ctcggcaagg acgcgaagga
     5581 cagcggcttc ttcgatcagt atccttactt cggccagctg tcggtaccgg ccggcaccta
     5641 tcgtggcgtg gataccgaga cgccttcgtt ccaggattcg gccctgtggg tggcgaactc
     5701 caaggtctcc gagcagacgg tctacgagat gctgtccgcg atctataccg acgagggcct
     5761 ggcacacatg aaggcgcaga aaaagacctt caagaacatg agcctggaat ccggtacaca
     5821 gggtgtggtt accccgtggc atcccggtgc ggtcaagttc tggaaagaaa agggcatgat
     5881 gtaacacgcc tgaaaggcgg ggcctgccct ggccccgcct tttcttgctt cgcagtcgcc
     5941 gggccgcgga tcggcattcg gaatcgacaa taacatcgag ggggatcggg gcatgttgta
     6001 cgaaaaactg cacaaggtcg aacgcctgat gttcgacgcc atgggaatcg ggctggtgct
     6061 gttctattcc tattccgccg tgctgcagcc ggcggccacc cagtatcatc gcggtattta
     6121 tgtcgtcatt acctacgtgc tggtgctgat gctttaccgc acaccgggag tggccgggcg
     6181 tgtctgggac tacttcctga tgctggtgtc ggtcgttgct ctgggctact ggatcgtcaa
     6241 cttcgaagcc atcaattacc gcgccggcgc cgaaaccgag ctcgaccagt gggtggcgat
     6301 ggccggggtg ctggtcggta tcgagatcgc gcgccgcgtg gtcggtaccg tgttcgtcgt
     6361 catcggcacg gtcatgctgc tgtacggagt gttcggcgac agcgaaatga taccggaagt
     6421 cctgcagcat gcgggcgaca cctttccgct gctgtgcacc agtatcttct acaagagcga
     6481 cggcgttttc ggcatcatgg ccaacgtgct ggcgacctat atcatcctgt tcgtgatctt
     6541 cggcgccttc ctcgaaaaga gcggcgcgca gcgcttcttc atcgactttc cgctggccgc
     6601 ggtcggtcac aagatcggcg gaccgggcaa ggtctcggtc atcgccagcg gcctgttcgg
     6661 ttcgatttcc ggcagtgcga tcgccaacac ggtgtcgacc ggtgcgttca ccattccgat
     6721 gatgaaaaag gccggcttca agccgcacgt ggcgggcggg atcgaaccgg cggcgtcgat
     6781 cggcgggatg ttcatgccgc cgatcatggg cgccggcgga ttcatcatgg cggagctgac
     6841 cgggttgccc tattcacaga tcatgctggt cgcgatcttt ccggcgctga tgtacttctt
     6901 cagcgtgttc atgatggttc attacgaggc caagaaggag aatatcgtcg gcgaaaaatc
     6961 gaagcggagc gccaaggaga ttctgcgcaa ggagtggttt tacatcctgc cgctggtggc
     7021 gatcacgatc ctgatgctga tgggctattc gccgggttac tcggccatcc tgggtatcgt
     7081 cacctgcatc gcggtgagct ggtttcgcaa ggatacgcgc atcgacctca agggtttcgt
     7141 cgacgcctcc cgctccggcg ccgaaaccag cctgaagatc ggcgccaccg tcggcgtcat
     7201 cggcatcatc atcggcgtgc tgacctacag cggcctgatc ctgacctttg ccgacatcgt
     7261 catcgatctc gccgagggac ggctgtggct gaccatcctg ctggtggcgc tcgcctcgct
     7321 ggtactggga atgggcgtgc cggtgaccgc ggcctacctg attaccgcgg tggtggcggt
     7381 gccggcgctg acccacctcg gggtcaacga actcgctgcg cacatgatcg tctactggct
     7441 gtcgcaggat tccaatatca cgccgccggt atgcatcgct gcgttcgccg gtgccactat
     7501 cgccaaagcc agcatgtggg cgaccgcgtg ggttgccttc aagtttgcca agttcctcta
     7561 cctggcgccg ttcatcttcg gttacgtgcc ggcgttctcg ctggacggtt cggcaatgga
     7621 tatcgccatc accttcgtgc tggtgttttt cggcacctgg gcctatagct ggttcctgag
     7681 cggaatctgg ctgaagcgta agccgaaagc cgcagccgcg ggagagcaga actgatgtca
     7741 aagcacaagc cgctaccgca aatcgattac cgcaagattc tctatgtcac cgacct
//
LOCUS       DATCZV010000233         7509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_282636, whole genome shotgun sequence.
ACCESSION   DATCZV010000233 DATCZV010000000
VERSION     DATCZV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282636"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2720)
                     /locus_tag="VIW27_05185"
     CDS             complement(<1..2720)
                     /locus_tag="VIW27_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5739095.1"
                     /translation="MSYRQRLCTIGYSLLTLLAFCFSPQAVSQDTQAWVATLKSVQGT
                     VETRKAEQTEWRPAALDDTFAIGDSVRVLGYSRAAILLPDETLLRLDQNTTVTFTPPR
                     DQNRSWLDLLKGAIHIISRDPRALRVVTPFAGAGLEGTEFLVEVGTDATTITVYEGEV
                     AMTGDAGDVNLGPGQRATARAGLQPLAEVAVRPRDAVQWTLYYAPLLNRKPPAADAQA
                     EGEQAENPQFYTGRAASRLAVGRVDGALADIATALRLDADDVEALALQAIIAVAQNDT
                     DTAFERANPALAHDADSAAALVALSYAQQAIFDISGALSTMQRAVDRHPSNGLALARL
                     SELWLAVGDIDKSVEVAEEAVEQNANVARAHTVLGFAYLARIDIPQARAAFDRAIVLD
                     QAAPLPRLGLGLAMIRDGDLEGGRAEIELAVILDPGNSLLRSYMGKAYYEERRDGLAA
                     SQLDLARKLDPLDPTPWFYDAIRQQTGNRPVEALADLEASVARNDNRAVYRSRAYLDQ
                     DRAVRGISQARVYEDLGFEQLGVLAGTRSLGIDPANHSAHRFLSDSYAAQPRHEIARA
                     SELLQSQLLQPINGSPVQPQLPEVDLNIVSGAGPAEAAFNEFTPLFEGNGPQLVVSGL
                     YGSNDTWSDEVVLSGIQDRVAYSIGQFHHESDGFRENNDIEHDIYNFFVQAELSTNID
                     VQFEFRRRETEQGDLRLNFDPDDFSVTERTNIDQDVGRLGFHLAPTPRSDFVVSLIKS
                     EREQIVNRMDPVAPIIESDLESESEDIQGQYLFRGDRFNITAGLGVSNIDVEGGNFID
                     ASPIFGGICPPSPPFFGVCASTTPVDQTTEQQNEYIYVNLIGPGKVTWTIGASHDSVE
                     DGPLDLDESNPKFGVQWEATNNIRLRAAYFETVKRTLVAEQTLEPTQVAG"
     gene            complement(3100..3666)
                     /locus_tag="VIW27_05190"
     CDS             complement(3100..3666)
                     /locus_tag="VIW27_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739096.1"
                     /translation="MKQALEKSAPIIEHLKSWLGIVAVIGAGVVTPWEYIDLKQEKRV
                     ERTLSYVERYGDGGYMDSAILLDEAIADRHEQLVAVLQNADLAAREIESAYSRVILGI
                     FDDRELRRAMNSVLRFQEELVQCVVNDLCDRKVALSFFSNSTGDMLRSYYPYICDKRA
                     RWQDQDAFRDVENFYLGESDTDICRNLN"
     gene            complement(3691..3906)
                     /locus_tag="VIW27_05195"
     CDS             complement(3691..3906)
                     /locus_tag="VIW27_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739097.1"
                     /translation="MNALKIPTLILCLLLGSPFAAAAGQAQSAEDGSQRAPASGPESA
                     EADDQGGDRGFDPCLLNANLAVCADRQ"
     gene            4081..4887
                     /locus_tag="VIW27_05200"
     CDS             4081..4887
                     /locus_tag="VIW27_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739098.1"
                     /translation="MNASQILPGFRSLCLALVIIISALPKALAAKPLDLHYIGGLSLG
                     YSSFDFPAKLDHKLTFPVYQLHGGIAYKKYYVVVNLADSFADADVSEEEDIGEAGRYD
                     RDISLGYQLTPSWGIFAGYKTGKTTIDYISREDQDAGGTVPVRQESYRHQGPFVGVSY
                     ARKFERAGRLSLSLAYADLDASNRFVRDADDEGEDEKPEFDDLTGSVEGDSTGLSFGA
                     RWSIPVAGNLLYYLSYRINDYQQDLTIDGNRFDNIDETITYLTTGLFFVF"
     gene            complement(4927..5808)
                     /locus_tag="VIW27_05205"
     CDS             complement(4927..5808)
                     /locus_tag="VIW27_05205"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY5739099.1"
                     /translation="MIDSKIPGRQQVTPQRGRLSIALLAGLALALSICPQLSAAAEMR
                     RMALLVGNADYYFAPLKNPLNDANDLGRELAAIGFETRVLENPGYAQFRDAIANFYDR
                     VGEDSNTLSVFYYAGHAVQMNNVNYLIAVDSKIVDQQTLIDGSISINDLLRDMQQTRN
                     GQNLIILDACRDNPFKAIEPGDQSRGVAMPDDEVRELQQGLAPVEAPAGTIIAYATAP
                     GGVAKDGKGRNGTYTRYLLEHLTEQRPVEEVFKRVRKDVMRASRGEQIPWEHSSLSKS
                     VYFYPPRNEEIPEIGGF"
     gene            complement(6188..6547)
                     /locus_tag="VIW27_05210"
     CDS             complement(6188..6547)
                     /locus_tag="VIW27_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEY5739100.1"
                     /translation="MHRGKAILTLAWLLLGGCAAHSSKPVIDPAGVDMAQYEKDLGEC
                     EQIALQVESKAGAGAAEGAVVGGLIGGIFGGSDGALKGAGAGAVGGAAGGAADTQREQ
                     STVVKNCLRNRGYQVLN"
     gene            complement(6713..7420)
                     /locus_tag="VIW27_05215"
     CDS             complement(6713..7420)
                     /locus_tag="VIW27_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739101.1"
                     /translation="MTIPIFRITGLILLISLVVLYPRLAAAQSPSDAAQIVDNIVRAY
                     GGRAALERMHGVRHSGQIQSYRLGKTGTLSRLFVLPGRLRVDLNYSDGPSEQRITTAD
                     GAWRDGRPATAPMHLAMQLQSARFRLPLLLTEGPTTLLGKSEGRLHLQMPLTDTTSME
                     VLVDPASWRIVRSMGQMNFNGMRMAFTADYSDFRNVDGVLFAHREELAAMGMPTGIAT
                     LERIEVNPDFGPLDFKP"
BASE COUNT         1339 a         2317 c         2379 g         1474 t
ORIGIN      
        1 aagccggcga cctgcgtcgg ctccagcgtc tgctcggcga ccagcgtccg ctttacagtt
       61 tcaaagtaag ctgcgcgcag gcgaatgttg ttagtagcct cccactgcac accaaatttg
      121 ggattggatt cgtccaggtc cagagggcca tcctccacgg agtcatgact cgcgccaatt
      181 gtccaggtta cttttccagg accgatcaga tttacgtata tgtactcatt ttgttgttcg
      241 gttgtctgat ctacgggggt tgtggatgcg caaaccccga agaacggcgg ggacggaggg
      301 caaattccgc caaaaattgg gctcgcatcg ataaaattgc ccccttcaac gtcgatgttc
      361 gacacgccca gcccggccgt aatattgaat cgatcacccc gaaacaggta ctgcccctga
      421 atgtcttcac tttcggactc aaggtcagat tcgatgattg gcgccacggg atccatccga
      481 tttacgattt gctcacgttc actcttgatt agcgatacga cgaaatcgga tcgcggcgtt
      541 ggtgcaaggt gaaagcccaa ccgcccgaca tcctggtcaa tgttcgttcg ttccgtgacg
      601 gagaagtcgt ccgggtcgaa gttgaggcgc aggtcaccct gttcggtctc gcgcctgcgg
      661 aattcgaatt gcacatcgat gtttgtgctg agttcggcct gcacgaagaa gttgtagatg
      721 tcgtgctcga tgtcgttgtt ttcgcgaaag ccgtcggact cgtggtggaa ctggccgatg
      781 ctgtaggcga cgcggtcctg aatgcccgat aacaccacct cgtcggacca ggtgtcgttg
      841 ctgccgtaga gaccggaaac caccagctgc ggcccgttgc cttcgaaaag cggagtaaat
      901 tcattgaacg cagcctcggc cgggccggct ccgctgacga tattgagatc gacctccggc
      961 agttgtggct gcaccggaga accgttgatc ggctgcagca attgcgattg cagcaattcg
     1021 ctggcgcggg cgatttcgtg acgcggttgc gccgcgtagc tgtcggacag aaagcgatgt
     1081 gccgagtggt ttgccggatc gataccgagc gatctcgtgc ccgcgagcac accgagttgc
     1141 tcgaagccga ggtcctcgta gacgcgtgcc tggctgatgc cgcggaccgc gcggtcctga
     1201 tccaggtagg cgcgcgaacg ataaacggcg cggttgtcgt ttctggcgac cgaagcctcg
     1261 aggtcggcca gcgcctcgac ggggcggttg ccggtttgct ggcgaatggc gtcataaaac
     1321 cacggcgtcg ggtccagcgg gtccagtttc ctggcaagat cgagttgcga cgccgcgagg
     1381 ccgtcgcgcc tttcctcgta gtaagccttg cccatgtagc tgcggagcag cgagttaccg
     1441 ggatcgagga tcaccgcgag ttcgatttcg gcgcggccgc cttcgaggtc gccgtcgcga
     1501 atcatggcga ggccgagacc gaggcgcggc agcggcgccg cctggtcgag gacgatagcc
     1561 ctgtcaaagg ctgcccttgc ctgcggaata tcgatgcgcg cgagataagc gaatccgagc
     1621 acggtgtgcg cacgggcgac attcgcattc tgctcgacgg cttcttctgc aacctcgacc
     1681 gatttatcga tatcgccgac ggcaagccag agttcggaca ggcgcgccag ggcgaggccg
     1741 ttcgacggat ggcgatcaac cgcgcgttgc atggtcgaca gcgcccccga aatatcgaag
     1801 atggcctgtt gcgcgtaaga cagcgcaacc agcgccgcgg ccgaatcggc gtcgtgcgcc
     1861 agcgcgggat tggcgcgttc gaacgcggtg tcggtatcgt tttgcgcgac cgcgatgatt
     1921 gcctgcagcg ccagcgcttc gacgtcgtct gcatccagcc gcagcgccgt ggcgatgtcg
     1981 gcgagtgcgc cgtcgacccg gccgacggca agtcggctgg cggcgcggcc ggtatagaac
     2041 tgcggatttt cggcctgttc gccctcggcc tgcgcatctg ccgccggtgg cttgcgattc
     2101 agtagcggcg cgtaatacag cgtccactgc acggcatccc gcggccgcac cgcgacctct
     2161 gccaggggct gcaagccggc gcgggcggtg gcgcgctgac cgggaccgag gttcacgtca
     2221 ccggcatcgc cggtcatcgc tacctcgccc tcgtagacgg tgatggtggt tgcatccgtg
     2281 ccgacctcga ccaggaattc cgtgccctcg agaccggcac cggcaaacgg tgtcaccacc
     2341 ctgagcgcgc gcgggtcgcg gctgatgatg tgaatcgcgc ctttcagcaa atcgagccac
     2401 gagcgattct ggtcgcgggg cggagtgaag gtgacggtgg tgttctggtc gaggcgcaga
     2461 agcgtttcgt cgggcagcag aatggcggcc cggctgtagc ccagcacgcg caccgagtcg
     2521 ccgatcgcga aggtgtcgtc gagcgcggcg ggccgccact cggtctgctc ggctttacgg
     2581 gtttcgacgg tgccctgcac cgacttcagc gtcgccaccc atgcctgggt gtcctgtgaa
     2641 acagcctgcg gcgagaagca aaacgccaat agcgtgagca gactgtaacc gatagtgcac
     2701 agtcgctgcc ggtaactcac tcgcatcccc ctcgccggtg tgtttccttc gcgcaatgcc
     2761 cgcgtgcagt gccccggctt tattgcttca cacttgccac gggattattt ttattctgaa
     2821 actgatggaa agtgggccca gaataccatt cctgactgcg aggtctagtg ttatttccgc
     2881 agcggccggc tgacgggtcg gtccgctgtc gacaggatcc gcggctgccc gcgcgtagtt
     2941 tgcgggtgtt ttggatgcaa ttaccaatcg ccaaaacccg gtgccgggcc cggctcgcgc
     3001 atactgaaac cggcttcgac ggcacggctg atactccgcc ggccggattt cgatcgactg
     3061 ccgggcaagg tgcgccaggc cgggtcgccg cgcacccgct cagttgagat tgcggcaaat
     3121 atccgtgtcc gattcgccca ggtaaaaatt ctcgacgtcg cggaatgcgt cctggtcctg
     3181 ccacctggcc cgcttgtcgc agatgtacgg gtagtaggac ctgagcatgt cgccggtact
     3241 gttggaaaaa aagctcagcg ccaccttccg gtcgcagagg tcgttgacga cgcactgcac
     3301 cagttcttcc tggaagcgca gcaccgagtt catcgcccgg cgcagttccc ggtcgtcgaa
     3361 gatgccgagg atgacccggc tgtaggccga ctcgatctcg cgcgcggcta aatccgcgtt
     3421 ttgcagcacg gccaccagct gctcgtggcg gtcggcaatc gcctcgtcga gcaggatcgc
     3481 ggaatccatg taaccgccgt cgccgtaacg ctcgacatag ctcagcgtgc gctcgacgcg
     3541 cttttcctgc ttgaggtcga tgtattccca gggggtgacg acgccggcgc caatcaccgc
     3601 gacgataccc agccagctct tcaggtgttc gatgatcggg gcgcttttct cgagagcctg
     3661 tttcaaaggg ggctgcctgg gtctcggggg ttactgccgg tcggcgcaga ccgccaggtt
     3721 tgcattcagc aggcaggggt cgaagccgcg gtcgccaccc tggtcgtcgg cctcggcgct
     3781 ttccggtccg gacgctggcg cgcgctgcga accgtcttca gcggattgcg cctggccggc
     3841 cgcggcggca aacggcgaac ccagcagcag gcacaggatc agggttggta ttttcaatgc
     3901 attcattgcc ttggcgatga tagtcgaagt attatcgagg tgtctcgaat agtattacaa
     3961 acgcccaggt taaggaagtt gggtggttgc attcctgcct gcacccggct ggccgccgtg
     4021 tcgaacgggc gagacgcact cacgggaatc actcggcaca gaccgggaac gggggacaat
     4081 ttgaacgcat cgcagatttt gccaggattc cgcagccttt gcctggcgct ggtcataatc
     4141 atcagtgcgc tgccaaaggc gctcgcggcc aagccgctcg acctgcatta catcggcggc
     4201 ctgtcgctgg gttacagcag cttcgacttt ccggcgaagc tggaccacaa gctcaccttt
     4261 ccggtctatc aactgcatgg cggcatcgcc tacaaaaagt actacgtcgt ggtcaatctc
     4321 gcggacagct ttgccgatgc cgatgtctcc gaggaagagg atatcggcga ggccggtcgc
     4381 tacgatcgcg atatcagcct gggataccag ttgacgccga gctggggaat tttcgccggc
     4441 tacaagacgg gcaagacgac gatcgattac atctcgcgcg aggaccagga cgcgggcggc
     4501 acggtaccgg tgcggcagga atcgtaccgt caccaggggc ctttcgtcgg tgtcagttac
     4561 gcgcgcaagt tcgagcgggc cgggcggctc agcctcagcc tcgcctatgc ggacctcgac
     4621 gccagcaatc gtttcgttcg cgatgccgac gacgagggcg aggacgaaaa gccggaattc
     4681 gacgatttga cgggcagcgt cgagggcgat tcgacggggc tgagcttcgg tgctcgctgg
     4741 agcattccgg tggcgggcaa cctgttgtat tacctcagct accgaatcaa cgattaccag
     4801 caggacctga ccatcgacgg caatcgcttc gacaacatcg acgagaccat cacctacctg
     4861 accaccggcc tgttcttcgt gttttgagtc ggcccgcgac cgccggcggt cggcgaaggg
     4921 cgcgcctcag aagccgccga tctcgggaat ttcctcgttc cgcggcgggt agaaataaac
     4981 cgatttcgac agcgacgaat gttcccacgg aatctgctcg ccgcgggatg cccgcatcac
     5041 gtccttgcgc acccgcttga atacctcttc aaccgggcgt tgctcggtaa ggtgctcgag
     5101 caggtagcgg gtgtaggtgc cgttgcggcc cttgccgtcc ttggccacgc cgcccggcgc
     5161 ggtggcatag gcgatgatgg tgccggccgg tgcctcgacc ggtgccagcc cctgttgcag
     5221 ctcgcgcacc tcgtcgtcgg gcatcgccac gccgcggctc tggtcaccgg gctcgatcgc
     5281 cttgaacgga ttatcgcggc aggcatcgag gataatcagg ttttgcccat tgcgggtttg
     5341 ctgcatgtcg cgcagcaggt cgttgatgct aatgctgccg tcgatcaggg tttgctggtc
     5401 gacgatcttc gaatccaccg cgatcaggta gttgacgttg ttcatctgca ccgcgtgacc
     5461 ggcatagtaa aacaccgaca acgtgttcga atcctcgccc acgcggtcgt agaaattcgc
     5521 gatcgcgtcg cgaaactgtg cgtagccggg gttttccagc accctggtct cgaagccgat
     5581 agcggcgagc tcgcgaccga gatcgttggc gtcgttgagt ggatttttca gcggcgcgaa
     5641 ataatagtcg gcattaccga ccagcaacgc catgcgccgc atctcggcgg ccgcggagag
     5701 ttgcgggcag atcgagagcg ccaacgccag cccggcgagc agggcgatgg agagccggcc
     5761 gcgctgcggc gtaacttgct gcctgccggg aattttgcta tcgatcatgt ccgctgtgcc
     5821 gaggttcgct ggtagtttgc agccggcagt ataaccgact ccgcggccgc gggggtttcg
     5881 ccgccgaaga ttgcggctgt cgacagcaag ccgctccgct cgccgaaccc ggatcattgc
     5941 ctccggattc tattcgatag gcgggtcgcg gtaaatcgat gctcggcgat atttggcatg
     6001 gcggcgggta aaggagtcac ggcagtcgac cgatcgagaa tcggcagcaa ccacggatcg
     6061 cagggccgcg cggctggttg ttgtgcattc gactcggcag ccgcggtcga ggtgaaatcc
     6121 gcagacagcc cgccaatggc ccggcaagcg gagacatcgg ctcgcgcgca cggcacggtt
     6181 tgcgccgcta gttcaggacc tggtaaccgc ggttacgcag gcagtttttt accaccgtgc
     6241 tttgctctcg ctgggtatcg gccgcaccgc ccgcggcgcc gccgacggcg cccgcgccgg
     6301 cccccttcag cgcgccgtcg ctaccgccga agatgccgcc gatcagaccg ccgaccaccg
     6361 ctccctcggc cgcgccggcg ccggccttcg actcgacctg cagagcgatc tgctcgcact
     6421 cgccgagatc tttctcgtac tgcgccatgt cgacaccggc cgggtcgatg accggtttcg
     6481 agctgtgcgc ggcgcagccg ccgagcagca gccatgccag ggttaaaatc gccttgcctc
     6541 ggtgcactgt attctcccgt tgccgcgacc ccggcctgtc ggcggggtga gcggcgctaa
     6601 atcgttatcg tcaatagtat gaaaatttta gcaccccggg atgacgattc cagcgaatgt
     6661 cttgcgcggc gcgtatcgtc ggccgcgcgt tagcgtttgc cgattgccaa ttttacggtt
     6721 tgaaatccag cggaccgaag tcgggattca cctcgatgcg ttcgagggtc gcgatgccgg
     6781 tcggcattcc catggccgcg agctcttctc gatgggcgaa cagcacgccg tcgacgttgc
     6841 gaaaatcgga gtaatcggcg gtaaaggcca tgcgcatgcc gttgaaattc atctgcccca
     6901 tcgagcgcac gatccgccag ctcgcgggat cgaccagcac ttccatcgag gtcgtgtcgg
     6961 tcagcggcat ctgcaggtgc aggcggccct cggacttccc cagcagggtc gtcgggccct
     7021 cggtcagcaa cagcggcaac cgaaaacgcg ccgactgcag ctgcatcgcc aaatgcatcg
     7081 gcgcggttgc cggtcggcca tcccgccagg cgccgtccgc ggtggttatc cgttgctcgc
     7141 ttggtccgtc cgaataattc aaatcgactc gcaagcgacc gggcaggacg aacagccggc
     7201 tcaatgttcc cgtcttgccc aggcgataag actggatctg gcccgagtgc cgcacgccgt
     7261 gcatgcgctc cagcgcggca cgcccaccgt atgcgcgcac gatgttgtcg acgatttgcg
     7321 ccgcgtcgga cgggctctgc gcggcagcca ggcgcggata gagcacgaca aggctgatca
     7381 acaggatcaa cccggtgatt cgaaatatag gtatcgtcat ctcgctgtcc tgctcgccga
     7441 cctgccgcgt tggtaagcgg ctcaatatac catcgaaagg cgtcctggtc cggctattct
     7501 cggactacc
//
LOCUS       DATCZV010000234         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_336104, whole genome shotgun sequence.
ACCESSION   DATCZV010000234 DATCZV010000000
VERSION     DATCZV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336104"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VIW27_05220"
     CDS             complement(<1..514)
                     /locus_tag="VIW27_05220"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5739102.1"
                     /translation="MRFRVKQLALGILVSVSLATAGPAQASTDPELRRLLKEAVESDY
                     GFEDRFDAQVWLLDMSNRLAKFVEDPQNRIDLLKQVHYEARRVDIEPELVLAVIEVES
                     HFDEYAISVSGARGLMQVMPFWLDEIGISDKNLFKIRTNLRMGCTILRYYIDMEPDDL
                     GRALARYNGSL"
     gene            complement(499..>2080)
                     /locus_tag="VIW27_05225"
     CDS             complement(499..>2080)
                     /locus_tag="VIW27_05225"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY5739103.1"
                     /translation="VLRKVEDIIRQEMDRAGALELLMPSFQPAELWQESDRWNQFGPE
                     LLRLKDRHQRDACIGPTHEEIVTDIFRREVSSYRQLPINFYQIQTKFRDEIRPRFGVM
                     RSREFIMKDAYSFDLDPAGMQRSYDIMDEAYTRIFDRFGLEYRAVSADSGAIGGSKSQ
                     EFHVLADSGEDAIAFSDQGDFAANIETVEALAPAGERSAPAAEMETVDTPGMYSIEDL
                     ANGLGISPAQCLKTLIAESTDGGLVALVLRGDHELNALKAQDLEGVASPLRLASREQI
                     QQQLGNEIGSIGPVDLGIQTFVDRSAAQLADFVCGANQNDKHFVNANWGRDCEEPRVV
                     DIRNVIDGDPSPDGKGQLRILRGIEVGHIFQLGQKYSQSMQATVLDEQGKSTVPYMGC
                     YGIGVTRVVAAAIEQNHDQQGIIWPATLAPFQLAICPINYHKSAEVRQAADEFYQVCL
                     DSGIEVLLDDRPLRPGVIFSDMELIGIPHRVVFSDRGLKARQFEYKGRRDAESRDLPL
                     DELSSFVKSLFPESCASA"
BASE COUNT          375 a          622 c          653 g          430 t
ORIGIN      
        1 cgaggctgcc gttgtaacgc gccagcgctc ggccgaggtc gtccggttcc atgtcgatgt
       61 aataacgcag aatggtgcaa cccatgcgca ggttggtgcg gatcttgaac aggttcttgt
      121 cggaaatgcc gatttcatcg agccagaagg gcattacctg catcaggccg cgggcgcccg
      181 aaaccgagat cgcgtactcg tcgaagtggc tttcgacctc gattaccgcg agtaccagtt
      241 cgggttcgat atcgacccgc cgcgcctcgt agtgcacctg cttgagcagg tcgatacggt
      301 tttgcggatc ttcgacgaac ttcgccagcc ggttagacat gtcgagcagc catacctggg
      361 cgtcgaagcg atcctcgaat ccgtagtcgg actcgaccgc ttcttttaac agccgcctga
      421 gttcggggtc ggtcgaggcc tgtgcggggc cggcggttgc cagcgatacc gacaccagga
      481 tgccaagtgc gagttgcttt acgcggaagc gcatgattcc gggaacagcg attttacgaa
      541 cgacgacaat tcgtcgagcg gcaggtcgcg tgattcggcg tcgcgccgtc ccttgtactc
      601 gaactgtcga gccttgaggc cgcgatcact aaacaccacg cggtggggta tgccgatcaa
      661 ttccatgtcg gaaaaaatca ccccgggacg cagcggccga tcgtcgagta gaacctcgat
      721 gccgctgtcg agacaaacct ggtaaaactc gtcggccgcc tggcgtacct cggcggactt
      781 gtggtagttg atcggacaga tcgcgagctg aaacggcgcc agcgtggcgg gccagatgat
      841 gccctgctga tcgtggttct gctcgatcgc ggctgccacc acgcgggtca cgccgatgcc
      901 gtagcaaccc atgtagggca ccgtcgattt gccctgttcg tcgagtaccg tcgcctgcat
      961 cgactgcgaa tacttttgac cgagctggaa gatatgtccg acctctatgc cgcgtaaaat
     1021 cctcagctgt cccttgccgt cggggctcgg gtcgccgtcg atcacgttgc gtatgtcgac
     1081 cacccgcggc tcctcgcagt cccggcccca attggcgttg acgaagtgct tgtcgttctg
     1141 gttggcgccg caaacgaaat cggcaagttg cgcggcactg cgatcgacga aggtctggat
     1201 gccgaggtcc acgggcccga tggagccgat ttcgttgccg agctgttgtt gaatctgttc
     1261 gcggctggcc agccgcagcg ggctggcgac gccctcgagg tcctgcgcct tgagggcgtt
     1321 cagttcgtgg tcgccgcgca gcaccagcgc caccaggccg ccatcggtcg attccgcgat
     1381 cagtgtcttc aggcattgcg cagggcttat tccgaggccg ttggccagat cctcgatgct
     1441 gtacatgccg ggcgtgtcga cggtttccat ttcggcggcc ggcgcggacc gctcgccggc
     1501 gggcgcaagt gcttcgacgg tctcgatgtt ggccgcgaaa tctccctggt cggaaaatgc
     1561 gatcgcatcc tcgccggagt cggcgagcac gtgaaactcc tgggatttac tgccgccgat
     1621 tgcgcccgag tcggcactaa ccgcgcggta ctcgagcccg aaacgatcga atatccgcgt
     1681 ataggcctcg tccatgatat cgtaggagcg ctgcataccg gccggatcga ggtcgaatga
     1741 ataagcgtct ttcatgatga attcgcgcga gcgcatgacg ccgaaccgcg gcctgatctc
     1801 gtcgcggaac ttggtctgga tctgatagaa attgatcggc agctgccggt aactgctgac
     1861 ttcccggcgg aaaatgtcgg tgacgatttc ctcgtgggtg gggccgatgc aggcatcgcg
     1921 ctggtggcgg tccttgaggc gcagcagctc gggcccgaac tggttccagc gatcggattc
     1981 ctgccacagt tcggcgggct gaaacgacgg catcagcaat tccagtgcgc cggcgcgatc
     2041 catttcctgg cgaatgatat cttcgacctt gcgcagaacc
//
LOCUS       DATCZV010000235         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_548752, whole genome shotgun sequence.
ACCESSION   DATCZV010000235 DATCZV010000000
VERSION     DATCZV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548752"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2547
                     /locus_tag="VIW27_05230"
     CDS             136..2547
                     /locus_tag="VIW27_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5739104.1"
                     /translation="MESQARVVIIGGGVVGCSILFHLAKLGWQDCVLLERNELTSGSS
                     WHAAGQIHTISSDPNISRLQGYTINLYRELEELSGQSVGLHSTGGFYAASNKDWYDYL
                     KRERSKARYMGLDQEFISLKELAEKHPLIDTSRYIAALWDPLDGDIDPSGVTYAYAKA
                     AKKLGASYHTHAAVIETNQRKDGSWDVVTKEGTIHAEHIVNAGGLWAREVGLLSGLHL
                     PVQPMEHHYLITETIPEIAAREERLPAGIDYEGNIYFRQEGQGMLLGTYEPKATPWMV
                     GGTPQNFGHELLEPQLDHIAERLELGFERIPALAEAGIKDMINGPFTFGPDGNPMIGP
                     VPGMRNYWAAVGVMAGFCQAGGVGKCLAEWMIDGEPEIDVWAMDIARFGDYASPDYGT
                     IKSSENYERRFLLTFPNETLPKGRRMRTTALYDRLTAKGAVWGDGFGLEAALWFADGA
                     DDAHEEPTFRRSRAHDYVAREVKAVREAVGVTEIANFAKHSISGPDARKFLDRVMAGR
                     LPPPGRIALSPMLTPKGKLYGDLTVACFADDDFMIFGSGAAQEMHRRWFESQLEGYHL
                     RYRNESRRYHGLGLSGPNARELLSRITRDDVSAQGLKFRDIRRSFVGTVPAILARLSF
                     SGELGYEIYVEPEYQLKLYEEIVAAGEDLGLTHYGLRALMSLRLEKNWGAWTLDFRPD
                     FTAAQAGLDAFIDWDKDFIGKAAAQKEREAGPTIRLVTMTIDTDGIDVSNDEAILQDG
                     DCVGYVTSGGYAHHVGKSMALGYVPAELAADGTALEVEINGETYAAKVVGKPLYDPAG
                     EKMRA"
BASE COUNT          542 a          919 c          907 g          500 t
ORIGIN      
        1 acgtccgcct tgctcggcac gaaaaatcct gcgtcaggtt acccaggttg gcgtcatccc
       61 ggcccccgag ccgggatctt gtaatttccc gaagttttca aaagacatga atcaagtgaa
      121 tttccggagt tgaatatgga atcgcaagca cgcgtagtga ttatcggcgg cggcgtggtc
      181 ggctgctcga tcctgttcca cctggcgaaa ctcggctggc aggattgcgt tctgctcgag
      241 cgcaacgaac tgacctcggg gtcgtcgtgg cacgcggccg gccagatcca tacgatctcg
      301 tcggacccga atatctcgcg cctgcagggc tatacgatca acctgtacag ggagctcgag
      361 gaactctccg ggcaatccgt cggcctgcac agtaccggcg gtttttatgc cgcgtccaac
      421 aaggactggt acgattattt gaagcgcgag cgctccaagg cgcgctacat gggtctcgat
      481 caggagttca tcagtctcaa ggagctcgct gaaaagcatc cgctgatcga taccagccgc
      541 tacatcgcgg cgttgtggga tccgctcgac ggcgacatcg atccgtcggg cgtgacctac
      601 gcctacgcca aggcggcgaa aaaactcggc gcgagttatc acacgcacgc ggcagtgatc
      661 gaaaccaatc agcgcaagga cggcagctgg gacgtggtga ccaaagaggg cacgatccac
      721 gccgagcaca tcgtcaacgc cggcggcctg tgggcacgcg aggtcggcct gctctccggc
      781 ctgcatctgc cggtgcagcc gatggagcac cactacctga tcaccgagac gatccccgag
      841 atcgccgcgc gcgaagagcg cctgccggcc ggcatcgact acgagggcaa tatctatttt
      901 cgccaggaag gccagggtat gctgctcggc acttacgagc cgaaggcgac accatggatg
      961 gtaggtggca cgccgcagaa cttcggccac gagctgctcg agccgcagct cgatcacatc
     1021 gccgagcgcc tcgaactcgg cttcgagcgc atcccggcgc tcgccgaggc cggtatcaag
     1081 gacatgatca acggcccatt caccttcggt cccgacggca acccgatgat cggcccggtg
     1141 ccgggcatgc gcaactactg ggccgcggtc ggcgtcatgg ccggtttttg ccaggccggc
     1201 ggggtcggca agtgccttgc cgagtggatg atcgacggcg agcccgagat cgacgtctgg
     1261 gcgatggaca tcgcgcgctt cggcgattac gcgtcgccgg attacggcac catcaagtcg
     1321 agcgaaaact acgaacgccg gtttctgctg accttcccca atgaaaccct gcccaagggc
     1381 cggcgcatgc gcactaccgc gctctacgac cggctgaccg cgaagggcgc ggtgtggggc
     1441 gatggcttcg gtctcgaggc cgcgctgtgg ttcgccgacg gagcggacga cgcgcacgag
     1501 gaaccgacct tccgccgctc gcgcgcacac gactatgtcg cgcgcgaggt gaaagccgtg
     1561 cgcgaggcgg tcggtgtcac cgagatcgcc aacttcgcca agcactcgat cagcggcccc
     1621 gacgcacgca agtttctcga ccgcgtcatg gccggacggc tgccgccgcc cggccgcatc
     1681 gcgctgtcgc cgatgctgac tccgaaaggc aagctttacg gtgacctgac cgttgcttgc
     1741 ttcgccgacg atgacttcat gatcttcggc tccggcgccg cgcaggaaat gcaccgccgc
     1801 tggttcgagt cgcagctgga aggttaccac ctgcgctacc gcaacgagtc gcgccgctac
     1861 cacggtctcg gcctctccgg ccccaacgcg cgcgaactgc tgtcgcgtat cacgcgcgac
     1921 gacgtttccg cgcaaggcct gaagttcagg gatattcggc gcagcttcgt cggcaccgtg
     1981 ccggcgatcc tcgcacggct gtcgttctcc ggtgaactcg gctacgagat ctacgtcgag
     2041 cccgaatacc agctcaagct ctacgaggaa atcgtcgccg ccggcgagga cctgggactg
     2101 acccactacg gcctgcgcgc gctgatgagc ctgcgcctgg aaaagaactg gggtgcgtgg
     2161 acgctcgact ttcggccgga cttcaccgcc gcgcaagcgg gtctcgacgc gttcatcgac
     2221 tgggacaagg attttatcgg caaggcagcg gcgcaaaagg aacgcgaggc cggcccgacg
     2281 atcaggctgg tgacgatgac catcgacacc gacggcatcg acgtctccaa cgacgaagcc
     2341 attctgcagg acggcgactg tgtcggctac gtgacctcgg gaggctacgc gcatcacgtc
     2401 ggcaaatcga tggcgctcgg ctacgtaccg gcagaactgg ccgccgacgg caccgcgctc
     2461 gaggtcgaaa tcaacggcga gacgtatgcc gcgaaggttg tcggtaagcc gctctacgac
     2521 ccggccggtg aaaagatgcg tgcctagata aagttgcccg tcgccgacaa ggtcctgctg
     2581 tacctgagga ccagcggcgc aatgcgcctc cacggtatcg ctgtcagcaa cgggcctcgc
     2641 cgggttcgaa ctgttttgct tagccccggg gtttcgcagc gataatcgct gcggactctg
     2701 gtacccgaag cacgtcttgc ccgttggctc gatgtgcggt gccggccccg gggtttgcat
     2761 gattgcgggc agggcagatt cgggaggagc gccgggctga aggacgtgtc cggtctctat
     2821 atactgcgcc ctgatacgcg gagcatttgc gcttactccg tctgtacc
//
LOCUS       DATCZV010000236        11324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_336227, whole genome shotgun sequence.
ACCESSION   DATCZV010000236 DATCZV010000000
VERSION     DATCZV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336227"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /gene="nadA"
                     /locus_tag="VIW27_05235"
     CDS             complement(<1..230)
                     /gene="nadA"
                     /locus_tag="VIW27_05235"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY5739105.1"
                     /translation="MVATAIDLQPYASLNDEDCERRITRARQILGDRVIILGHHYQRD
                     EVFKHADFSGDSLKLSREAAASKAEYIVFCGV"
     gene            complement(308..2086)
                     /gene="aspS"
                     /locus_tag="VIW27_05240"
     CDS             complement(308..2086)
                     /gene="aspS"
                     /locus_tag="VIW27_05240"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY5739106.1"
                     /translation="MRSHYCGELTRQQVDQQISVCGWVNRRRDHGGVIFVDLRDRKGM
                     LQVVFDPDDAAMFADAETLRNEFVLRVEGRLRLRPEGTENPEMATGEVELLAQRLEIL
                     NASETPPFQLDDDDVHDDIRLRYRYIDLRRPEMQQRMQLRARVTQFLREWLTANEFLD
                     IETPMLTKATPEGARDYLVPSRTHPGSFFALPQSPQLFKQLLMMSGMDRYYQIVRCFR
                     DEDLRADRQPEFTQLDIETTFLDEQGIMQLMETMMRTLFVELLGADVEDSFPHMSYAE
                     AMERYGSDKPDLRIDLELKTVSDLLQDVEFKVFSGPASDPRGRVAALRVPGGNSLTRK
                     EIDDYTDFVGRYGARGLAYIKCNDVAQGREGLQSPILKFLPDEAIAGILERTGAASGD
                     LIFFGADRANVVNESLGALRVRLGHDLGRVEPGWRFLWVVDFPMFEYDEREDRLNALH
                     HPFTAPATGDPDELEAHAGEMLSRAYDMVLNGTELGGGSVRIHRMEMQQRVFKLLGID
                     EQEAEEKFGFLLTALRYGCPPHGGIAFGLDRLVMLMTGAASIRDVMAFPKTQTASCLL
                     TQAPSPVSERQLRELGIRLRKASSDT"
     gene            complement(2147..2458)
                     /locus_tag="VIW27_05245"
     CDS             complement(2147..2458)
                     /locus_tag="VIW27_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5739107.1"
                     /translation="MPIYEYVCNECGHQFEALQKLSDAALKTCPGCDKDSLKKKISAA
                     GFRLSGSGWYETDFKSDRKKNLAQGDSSQDGAGKGEQGKPAASGSEKPGKTDGAKEAV
                     A"
     gene            complement(2580..3353)
                     /locus_tag="VIW27_05250"
     CDS             complement(2580..3353)
                     /locus_tag="VIW27_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739108.1"
                     /translation="MKFTGAALKTKLKATAIHMSMSIAVFAFLAYQIYYNWYPQPYFE
                     IDGGWQGIRLIAAVDLVLGPVITFLIFDLSKRMRAIIFDLVVILVIQFGALAYGVYVT
                     YTQRPIAVVLIDEFVVPAIMEHYGGKLESTEQLSRYSDEKPPIIYADLPLDEAQLAEI
                     NRIKIEEKVLENAQMQLYRPQSGLREALDRLQPVFYDRLDFFGARAALEEWLETHGKS
                     ADEVLIARFEGRFGNAWLVFDRRGEYLSYFLNPESTVVE"
     gene            3391..4173
                     /locus_tag="VIW27_05255"
     CDS             3391..4173
                     /locus_tag="VIW27_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739109.1"
                     /translation="MEAVPEKFKIRTAYNEFHVFRHQGLLELRSGDNSLQSAIDPRAP
                     QRLCLRNLEHLMTVLLFIPEPRNILVLGTAAGSLLHYLRHYLPAARLTAVDIDTELIA
                     QLLQLGVLPPRDDRLDYVTADAADFIAGCEQRFDLVLVDLFNGARSPAWLLERESSDA
                     LYRLCSERGAVAYNLLLASEHDFARFYRELRQLYRGLTLSLPVQGFENRIVCAVRERA
                     PENVDMNARLQQAVALAERLQLDFPRLLAVAYNSNPAGVGLL"
     gene            complement(4207..4521)
                     /locus_tag="VIW27_05260"
     CDS             complement(4207..4521)
                     /locus_tag="VIW27_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY5739110.1"
                     /translation="MTMQAIIEDKLASEFDAFYLQVENESHMHNVAPGSESHFKVTIV
                     SDSFRDQMLIKRHRRVNQVLQDELRQIHALALHTLTLDEWRARGGTVAASPKCQGGGR
                     NQ"
     gene            complement(4541..6838)
                     /locus_tag="VIW27_05265"
     CDS             complement(4541..6838)
                     /locus_tag="VIW27_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5739111.1"
                     /translation="MTEKLSQATKESVDRLLQVIPETSAPHSEILRKICRDQVEGGSE
                     DLFLGKLDDEATEIRSTAASILSETGQINPNKLFSKLHQPNVSQSEIIEILFFQRESL
                     NPAQIIANALKLDKGHAEQLLKLLPDNRQPLDIPALQLEPVAIKNPSVKIGLLRYFAG
                     IEEPAAAELISRFLVDPNHTVVMEALRSLRQLDVRFDATFMLQSVEKMNEAELEMASE
                     ILEKQADFKLVPKVAPFVCGKSDEVRQRLIRLFVEHVSPKGLEIFLTLLEPQDWWGRD
                     QALKCLQQFANAKVADAAKTLVEDNNEFIRDQAQQLAASSNDASDLKQLWQNAIHENW
                     QVRDNALQAIGRSQKREAIPLLKKVLETRPESTSAVLKAAAQLGFSKGLEIAFSCLRM
                     PEALIQREALETIGKLTNERHARLVRDKLMQRVPQLQATVRDTAGEVINRLTDDYQLP
                     ALEIDQETYFDTRLVRFEDTQTIEQYKATAEEPEINIEDLKKGDLWIDRYRIDREIGR
                     GAMGRVMLATDEVVEETVILKFMHPELTADTDSRERFLREVKYSRKISHPNVIRVHDM
                     LFRNNLSAISMEFFPSRGLDEMLRQQKHFEVSEALDILLQVANGMAAAHDQQVIHRDL
                     KPSNILMNDAGLVKIVDFGIASASSQSEGTLTKSGSIIGTPAYISPERARGKDSDHRS
                     DIYALGIIAYGMLSGRLPYNGEPMSLLYQHIEGNAVPVHEIRKEVGARVSLLVQKMMA
                     VDINDRLQTMQDVAEAIKGLQRKSA"
     gene            complement(7037..9625)
                     /gene="clpB"
                     /locus_tag="VIW27_05270"
     CDS             complement(7037..9625)
                     /gene="clpB"
                     /locus_tag="VIW27_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY5739112.1"
                     /translation="MRMDRLTSKFQDALSDAQSLAVGRDHQMIEPVHLMIALLDQQGG
                     SVRPLFSQAGVNINLLRSQLGEMLERLPRVEGAGGEIHVSNNLGKLLNVTDKLAQERK
                     DQFISSELFVLAALDDKGPLGDALRAAGADQKMLAAAVDAIRGGQTVDNANAEEQRMA
                     LEKYTIDLTERAEQGKLDPVIGRDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINSEVPEGLKHKRVLSLDMGALIAGAKFRGEFEERLKAVLHDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVQEPSVEDTIAILRGLKERYEVHHGVDITDPAIVAAATLSYRYIADRQLPDKAID
                     LIDESASRIRMQIDSKPEPMDRLERRLIQLKIEREALKKESDSASKKRLAALQDEISS
                     LEREYSDLEEVWKSEKAALQGSTHIKEELERARLDLETAQRAGDLARMSELQYGRIPE
                     LEKQLNAAANFELQDMQLLVNKVGEEEIAEVVSRWTGIPVSKMLESEREKLLDMETRL
                     SERVVGQHEAVKAVSDAIRRSRAGLSDPSRPNGSFLFLGPTGVGKTELTKALTHFLFD
                     SEDAMIRLDMSEFMEKHSVARLVGAPPGYVGYEQGGYLTEAVRRRPYSVILLDEIEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNCVVVMTSNLGSTEIQAMAGDQHYDEIKRA
                     VMEQVGRHFRPEFINRIDETVVFHPLTREQIRSIAQIQLQSLSARLKDKDIELSISDA
                     ALDTLGELGFDPVYGARPLKREIQQALENPIAMSILSAEFEPGDRIAVEIDSEGRFEF
                     SKRRLH"
     gene            complement(9705..10433)
                     /gene="pgeF"
                     /locus_tag="VIW27_05275"
     CDS             complement(9705..10433)
                     /gene="pgeF"
                     /locus_tag="VIW27_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY5739113.1"
                     /translation="MIELIEPLWTAPPAVRSAFTTRGGGFSEPPYAGFNLGLHVGDDP
                     ARVARNRALLRSTLDLPAEPSWIAQTHGTRAVVLEAETRRDADAAITRVPGQVAVVMV
                     ADCLPILLCNRDGSEVAAVHAGWRGLQAGVVASTLGRMRSSAAQLLAWIGPGISRDNF
                     EVGDEVLEAFGESFDGARDFFEAHGEGHWMCDLGGLAERELERLGVTEIWRDPRCSYG
                     GARQFYSYRRDRVTGRMAALIWID"
     gene            complement(10426..>11324)
                     /gene="rluD"
                     /locus_tag="VIW27_05280"
     CDS             complement(10426..>11324)
                     /gene="rluD"
                     /locus_tag="VIW27_05280"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEY5739114.1"
                     /translation="SRLDQALQQLLPDYSRSLIQGWIQQGFVSINRQLCKPRQKVFPG
                     DRVDLDVPEQARIADPPQAVEFDLLHEDDDILVVDKPAGLVVHPAAGHQDGTLVNGLL
                     AHDPRLEQLPRAGIVHRLDKDTTGVMVVARNLRAHRWLVEELQARRVKREYVAIARGV
                     VTAGRSIELAIGRHPQQRKKMSVQKSGKPALTHFQVVRKFAHYSLLRLQLETGRTHQI
                     RVHLAHLHHPLLGDPVYGGRNRIPAGLNAALSERIAAFRRQALHAERLSFVHPATREP
                     VSFEAPLAADFEHLLEALEQYD"
BASE COUNT         2024 a         3554 c         3421 g         2325 t
ORIGIN      
        1 tgcacgccgc aaaagacgat atattcggcc ttcgaagccg cggcctcgcg cgacagcttc
       61 agcgagtcgc cggaaaaatc agcgtgcttg aaaacctcgt cgcgctggta gtggtggccg
      121 agaatgatca ccctgtcgcc caggatttgc cgtgcgcgcg tgatgcggcg ctcgcaatcc
      181 tcgtcattca acgacgcata gggctgtaaa tcgatcgcgg tggctaccat ttcggactct
      241 gacgggcgga cgaacggcca atatatcatc tgccgtaatg gcttgccaat cggcccggcg
      301 ccgggaatca ggtgtcgcta ctggccttgc gcaggcgaat gccgagttcg cgcagctggc
      361 gctcactgac cggtgacggc gcctgcgtca acaggcagga agccgtctgc gtcttcggaa
      421 acgccatcac gtcacggatc gacgccgcgc ccgtcatcag catcaccagc cggtcgaggc
      481 cgaaggcgat gccaccgtgg ggcgggcaac cgtaacgcag ggcggtcagc agaaagccga
      541 atttttcctc ggcttcctgc tcgtcgatac cgagcaactt gaacacgcgc tgctgcattt
      601 ccatgcggtg aatacgcacc gagccgccgc cgagctcggt gccgtttaac accatgtcat
      661 aggcgcggga cagcatttcg ccggcatgcg cctcgagctc gtccggatcg ccggtcgccg
      721 gcgcggtaaa cggatgatgc agtgcattga gacggtcttc gcgctcgtcg tactcgaaca
      781 tcggaaagtc gaccacccac aggaaacgcc agcccggctc gacccggccg aggtcatgac
      841 ccagcctgac gcgcagtgcg cccagcgatt cgttaaccac gttcgcgcgg tcggcgccga
      901 aaaagatcag gtcgccgctg gcggcaccgg tgcgctcgag aatgccggct atggcctcgt
      961 ccggcaggaa cttgaggatc ggcgactgca gcccctcgcg accctgcgcg acatcgttac
     1021 atttgatgta agcgagcccc ctggccccgt agcggccgac gaaatcggtg tagtcgtcga
     1081 tctccttgcg cgtcaatgaa ttgccgccgg gcacccgcag cgcggcgacc cggccgcgcg
     1141 gatcgctggc aggacccgag aagaccttga actcgacgtc ctgtaacaaa tcggaaaccg
     1201 tcttcagctc caggtcgatg cgcaggtcgg gcttgtccga gccgtagcgc tccatggcct
     1261 cggcgtaact catgtgcggg aacgaatcct cgacgtcagc gcccagcaat tcgacgaaca
     1321 gcgtgcgcat catggtttcc atcagttgca tgatgccctg ctcgtcgaga aaagtggtct
     1381 cgatatcgag ctgcgtgaac tcgggctggc gatcggcgcg caggtcctcg tcgcgaaagc
     1441 agcgtacgat ctggtaatag cggtccatgc ccgacatcat cagcaactgc ttgaacagct
     1501 gtggcgactg cggcagcgcg aagaaactgc cggggtgggt tcggctcggc accaggtaat
     1561 cgcgcgcacc ctcgggggtc gccttggtca gcatcggggt ttcgatgtcg agaaactcgt
     1621 tggcggtcag ccattcgcgc aggaactgcg taacgcgggc gcgcaattgc atgcgctgct
     1681 gcatctcggg gcggcgcagg tcgatgtagc gatagcgcag ccgaatgtcg tcgtgcacgt
     1741 cgtcgtcgtc gagctggaac ggcggggtct ccgacgcgtt gaggatttcg agccgctgcg
     1801 ccagcagctc gacttcgccg gtcgccattt cggggttttc ggtaccctcg ggccgtagcc
     1861 gcaatctgcc ctcgacgcgc agcacgaatt cgttgcgcag ggtttcagca tcggcgaaca
     1921 tcgccgcatc gtcgggatcg aacaccacct gcagcatgcc cttgcggtcg cgcagatcga
     1981 cgaaaatgac gccgccgtga tcgcggcggc gattgaccca accgcaaacg ctgatttgct
     2041 ggtcgacctg ctggcgtgtg agctcgccgc aatagtggct gcgcataatg actggtccga
     2101 attaggtttc gtgatatcgc gccggtgacg ctcggcacga ggttattcag gcgacggcct
     2161 ctttggcacc atcggtcttg cccggctttt cgctgccgct ggcggcgggc ttgccctgct
     2221 cgcccttgcc ggcgccgtct tgcgagctat cgccctgcgc caggttcttc ttgcggtccg
     2281 acttgaaatc ggtttcgtac cagccagaac cgctcaacct gaacccggcc gcggaaatct
     2341 tctttttcag gctatccttg tcgcagccgg gacaggtctt cagcgccgcg tcgctgagtt
     2401 tttgcaaggc ttcgaactgg tgcccgcatt cattgcatac gtattcgtaa attggcatcg
     2461 tggtctccga aaaacagtcc ggttcaactt tccggcagta gcgcacaacg cgcaaaaaag
     2521 gcaccggatt ctataatagt ccgcccgaat ttccaaggct cgatcctgcg ctgcgccatt
     2581 tattccacga cggtcgattc ggggttgaga aagtaggaca ggtattcacc ccggcgatcg
     2641 aataccagcc acgcattgcc gaagcggcct tcgaagcgcg cgatcagcac ttcgtcggcg
     2701 ctcttgccat gggtttcgag ccattcctcg agcgccgcgc gagcgccgaa gaaatccagc
     2761 cgatcgtaga acaccggttg cagcctgtcg agtgcttcgc gcaacccgga ctgcggccga
     2821 tagagctgca tctgcgcatt ttccagcacc ttttcctcga tcttgatgcg gttgatttca
     2881 gcgagctgcg cctcatccag gggcagatcc gcgtagatga tcggcggctt ttcatcgctg
     2941 tagcggctca attgctcggt ggattcgagc ttgccgccgt aatgctccat gatcgccggg
     3001 acgacgaatt cgtcgatcag cacgaccgcg atcggtcgct gcgtataagt cacgtaaact
     3061 ccgtaggcaa gcgcaccgaa ctggatcacg aggattacca cgaggtcgaa tatgattgcc
     3121 cgcatccgct tgctgaggtc gaaaatcagg aaggtaatga ccgggccgag caccaggtcg
     3181 acagccgcga tcaggcgaat gccctgccag cccccgtcga tttcgaaata gggctgcggg
     3241 taccagttgt aataaatctg gtaggccaga aaggcgaaca ccgcgatact catcgacatg
     3301 tgtatcgcgg tcgcttttag cttggttttc agcgctgcgc cggtaaattt cattatactt
     3361 cgctccgctt acaacacgcg catatagaat atggaagctg ttccggaaaa attcaagatt
     3421 cgaaccgctt ataacgagtt tcacgtattc cgtcaccagg gattgctcga gctgcgcagc
     3481 ggcgataatt ccctgcaaag cgccatcgat ccgcgggcac cgcagcggct gtgcctcaga
     3541 aacctcgaac acctgatgac cgtgctgctg ttcattcccg agccgcgcaa tatcctcgtg
     3601 ctcggcacag cggccggttc gctgcttcat tacctgcgcc attacctgcc cgcggcccgg
     3661 cttaccgcgg tcgatatcga taccgaactc atcgcgcagc tgctacaact gggggtgctg
     3721 cctccccgcg acgaccggct cgattacgta accgcggacg cggccgattt catcgccggt
     3781 tgcgagcagc gattcgacct ggtgctggtg gacctgttca acggtgcccg ctcgccggcc
     3841 tggctgctgg agcgcgaatc gagcgacgcc ctttaccgcc tgtgcagcga acgaggagcg
     3901 gtcgcctaca acctgctgct cgccagcgag catgacttcg cgcggtttta ccgcgagctg
     3961 cgccagcttt accgcgggct gacgctgtcg ctgccagtgc agggtttcga aaatcgcatc
     4021 gtttgcgcgg ttcgtgagcg cgctcccgaa aacgtcgaca tgaatgcccg tctgcaacag
     4081 gccgttgccc tggcggagcg cctgcaactc gactttccgc gcttgctggc ggtggcttac
     4141 aacagcaatc cggcgggagt cggactactg tagcccgcaa tcccggccag acccggggaa
     4201 tcgaagctac tggttcctgc cgccgccctg gcacttcggg gaagcggcca ccgtgccgcc
     4261 gcgcgcccgc cattcgtcca gggtcagcgt gtgcagcgcg agcgcgtgga tttgccgcaa
     4321 ctcgtcctgc aacacctggt tgacgcgacg gtggcgcttg atcagcatct ggtcgcgaaa
     4381 gctgtcgctg acgatggtga ccttgaaatg ggattccgaa cccggcgcga cattgtgcat
     4441 gtggctttcg ttttccacct gcaggtagaa agcgtcgaac tcgctggcga gcttgtcttc
     4501 gataatggct tgcatggtca tggcgaccga ttatacagct tcaggcggat ttgcgctgca
     4561 atcccttgat cgcctcggcg acgtcctgca tggtttgcag gcgatcgttg atgtcgacgg
     4621 ccatcatctt ttgcaccagc aggctgacgc gggcaccgac ttccttgcgg atttcgtgta
     4681 ccggcaccgc gttgccctcg atatgctggt agagcagcga catcggctcg ccgttgtagg
     4741 gcaggcggcc gctgagcatg ccgtaagcga tgatgccgag agcgtagata tcgctgcggt
     4801 gatcggaatc cttgccgcgc gcgcgctccg gcgaaatata ggccggagtg ccgatgatcg
     4861 agccggactt ggtcagggtc ccctcggatt gcgacgacgc cgaggcgatg ccgaaatcga
     4921 cgatcttgac cagcccggca tcgttcatca ggatgttgct tggcttcaga tcgcggtgaa
     4981 tgacctgctg gtcgtgcgcc gccgccatgc cgttggcgac ctgcaacagg atatcgagcg
     5041 cctcgctgac ctcgaagtgc ttttgctgac gcagcatctc gtcgagcccg cggctcggga
     5101 aaaactccat cgagattgcg ctcaggttgt tgcgaaacag catatcgtgc acgcgaatga
     5161 cattcggatg actgatcttg cgcgaatact tgacctcgcg caggaatcgt tcgcgggaat
     5221 cggtgtcggc ggtcagctcc gggtgcatga acttcagtat caccgtttcc tcgaccacct
     5281 cgtcggtggc gagcatgacc cgccccatgg cgccgcggcc gatttccctg tcgatgcggt
     5341 agcgatcgat ccacaggtct ccctttttca ggtcctcgat gttgatctcg ggctcttcgg
     5401 cggtagcctt gtattgctct atcgtctgcg tatcttcgaa tctgaccagg cgggtatcga
     5461 aataggtttc ctggtcgatt tccagcgccg gcaactggta atcgtcggtt agccgattga
     5521 tgacctcgcc ggcggtatcg cgcacggtgg cctgcaattg cggaaccctt tgcatcagct
     5581 tgtcgcgtac cagccgcgcg tgacgctcgt tggtcagctt gccgatggtt tccagcgcct
     5641 cgcgctgtat cagggcttct ggcattcgca ggcaggagaa tgcgatttcc agccccttgg
     5701 agaatccgag ctgcgcggcg gccttcaata ccgcgctggt cgattccggc cgggtttcga
     5761 ggaccttttt cagcaatggg atggcttcgc gcttttgcga gcggccgatt gcctgcagtg
     5821 cattgtcgcg cacctgccag ttttcgtgta tcgcgttttg ccacaactgc ttgaggtcgg
     5881 atgcatcgtt actgctggcc gcaagttgct gcgcctggtc acgaataaac tcattattgt
     5941 cttcgaccag ggtcttggcg gcgtcggcga ccttggcatt ggcgaactgc tgcaggcatt
     6001 tgagggcctg gtcccggccc caccagtcct gcggttcgag cagggtgagg aatatttcca
     6061 gtcccttggg cgagacgtgc tcgacgaaca gccgaatcaa ccgctggcgc acttcgtccg
     6121 acttgccgca tacgaacggc gccaccttcg gaaccagctt gaaatcggcc tgcttttcga
     6181 ggatttcaga agccatttcc agctcggcct cgttcatttt ttccaccgac tgcagcataa
     6241 aggtggcatc gaaccgaaca tccagctggc gcagcgagcg cagcgcctcc atcaccacgg
     6301 tatggttggg gtccaccagg aacctgctga tcaactcggc ggccgcgggc tcctcgatgc
     6361 cggcgaaata acgcagcagc ccgattttta ccgacgggtt cttgatcgcc acgggctcga
     6421 gttgcagcgc cgggatgtct agcggctgcc tgttatccgg caacagcttg agcaactgct
     6481 cggcatgccc cttgtccagc ttgagcgcgt tggcgatgat ttgcgccgga ttcaggcttt
     6541 cgcgctgaaa aaacaggatc tcgataattt ccgactgcga gacgttcggc tgatgcagct
     6601 tgctgaacag tttgttgggg ttgatctgcc cggtctccga caggatcgac gccgcggtgg
     6661 aacgaatctc ggtggcttcg tcgtcgagct tgccaaggaa cagatcttcc gatccgccct
     6721 cgacctggtc gcggcaaatc ttgcgcagta tctcgctatg cggcgccgag gtttccggga
     6781 tcacctgcag caggcggtcg accgattcct ttgtcgcctg gctcagtttt tcggtcaaag
     6841 cgacgccctt cgcactattc agatcctcac actcgaggaa ctgcttgagc gtgcgattga
     6901 acataatgga gcctagaacc gccattttcg tgtcgttccg cccgaagccc tgcaggaagt
     6961 taccgtttac acgggattat agtcacaaaa tggctcaata ctgcctattc gcgggcttat
     7021 ttctgcgcaa cagttctcag tgcaggcgcc gcttcgaaaa ctcgaagcgg ccctcgctgt
     7081 cgatctcgac tgcaattcga tcgccgggct cgaattctgc ggacaggatc gacatcgcga
     7141 tcgggttctc gagcgcctgc tgtatctcgc gtttcaacgg ccgtgccccg taaaccggat
     7201 cgaaaccgag ctcgccgagc gtatccagcg ccgcgtcact gatcgacagc tcgatatcct
     7261 tgtccttgag gcgcgcgctc aggctttgca gctgaatctg cgcgatcgag cggatctgct
     7321 cgcgggtcaa cgggtggaag accacggttt catcgatgcg gttgatgaac tcgggccgaa
     7381 aatgccgtcc gacctgttcc attaccgcac gcttgatttc gtcgtaatgc tggtcgccgg
     7441 ccatcgcctg gatctcggtc gagccgaggt tcgaggtcat caccaccaca cagttgcgga
     7501 agtccacggt gcgtccctgg ccgtcggtga ggcggccgtc gtccagcacc tgcaacagta
     7561 tattgaacac gtccgggtgt gccttttcga tttcatcgag caggatcacc gagtagggcc
     7621 ggcggcgtac cgcctcggtc aggtaaccac cctgctcgta gccgacatag cccggcgggg
     7681 caccaaccag ccgcgcgacg ctgtgctttt ccatgaattc cgacatgtcg agacggatca
     7741 tcgcgtcctc gctgtcgaat agaaaatgcg tcaacgcctt ggtcaactcg gtcttaccga
     7801 ccccggtagg accgaggaac aggaacgaac cattggggcg cgacgggtcc gacagcccgg
     7861 cacgcgaacg ccgaatggcg tcggataccg ctttcaccgc ctcgtgctgg ccgaccacgc
     7921 gctcgctgag ccgggtttcc atgtcgagca gtttttcgcg ctcggattcg agcatcttgg
     7981 aaaccggaat gccggtccag cgagagacca cctcggcgat ctcttcctcg ccgaccttgt
     8041 tgaccagcag ctgcatgtcc tgcaactcga aattggcggc cgcattgagc tgtttttcca
     8101 gctccgggat gcgcccgtac tgcaattccg acatgcgcgc caggtcgccg gcacgctgtg
     8161 cggtttcgag atcgaggcgc gcgcgctcca gctcttcctt gatatgggtg ctgccctgca
     8221 aggcggcctt ttccgacttc cagacttcct cgagatcgga gtactcgcgc tcgaggctgg
     8281 aaatctcgtc ctgcagcgcc gccagccgct tcttgctcgc cgaatcgctt tccttcttca
     8341 gcgcctcgcg ctcgatcttg agctggatca ggcgccgctc gaggcggtcc atcggctccg
     8401 gcttggaatc gatctgcatg cgaatgcggc tcgccgactc gtcgatcagg tcgatcgcct
     8461 tgtcaggcag ctggcggtcg gcaatgtaac ggtaagacag ggttgccgcg gcgacgatcg
     8521 ccgggtcggt gatatcgaca ccgtggtgca cctcgtaacg ttccttgagg ccgcgcagga
     8581 tggcgatcgt gtcttcgacg ctgggttcct gcaccagcac cttctggaaa cggcgctcga
     8641 gcgcggcatc cttttcgatg tactggcggt attcgtcgag cgtggtggca ccgatacagt
     8701 gcaactcgcc gcgcgccagc gcgggtttaa gcatgttgcc ggcgtccatc gcgccctcgg
     8761 ccttgccggc gccgaccatg gtgtgcagct cgtcgatgaa caggataatc tgaccttcct
     8821 gtttggcgag gtcgtgcagt accgccttca aacgctcttc gaactcgccg cgaaacttgg
     8881 cgccggcgat cagcgcgccc atgtcgagcg acagtacgcg cttgtgtttc aggccttcgg
     8941 gcacctcgct gttgatgatg cgctgcgcca ggccttcgac gatcgcggtc ttgccaacac
     9001 cgggttcgcc gatcagcacc gggttgttct tggtacgccg ctgcaacacc tggatcgcgc
     9061 ggcgaatttc ctcgtcgcgg ccgatcaccg ggtcgagctt gccctgctcg gctcgctcgg
     9121 tcaggtcgat ggtgtatttt tccagcgcca tgcgctgctc ctcggcatta gcgttatcga
     9181 cggtctgtcc gccgcgaatg gcatctaccg cggccgccag catcttctgg tcggcgcccg
     9241 ccgcgcgcag cgcatcgccc aacgggccct tgtcgtcgag ggcagcgagc acgaacagtt
     9301 cgctggaaat gaactggtcc ttgcgctcct gcgccagctt gtcagtgacg ttgagcaact
     9361 tgccgaggtt gttggaaacg tgtatttcac cgcccgcgcc ctcgacccgc ggcagccgct
     9421 cgagcatttc gccgagctgc gaacgcagca ggttgatatt gacgccggcc tgcgagaaca
     9481 gcggtcgcac cgatccgccc tgttggtcta gcagcgcgat catcaggtgc accggctcga
     9541 tcatctggtg gtcgcggccg actgccagcg actgcgcatc ggacagtgcg tcctggaatt
     9601 tactggtcaa gcgatccata cgcatgggat catcctcata aacactaaaa attatatggg
     9661 tatgtggtac tgcccgggcc tttttcaagc cgccgggcgg atgatcaatc gatccagatc
     9721 agggccgcca tccggccggt gacgcggtcg cgccggtagg aatagaattg tcgcgcaccg
     9781 ccgtagctgc agcgcggatc gcgccagatc tcggtgacgc cgagccgctc gagttcgcgc
     9841 tcggcaagcc cgccgaggtc gcacatccag tgaccctcgc cgtgcgcttc gaaaaagtcc
     9901 cgcgcaccgt cgaacgactc cccgaatgct tccagcactt cgtcgccaac ctcgaaattg
     9961 tctcgcgaga tgcccggccc gatccaggcc agcagctgtg ccgcggatga gcgcatgcgc
    10021 ccgagggtcg aggcgacgac cccggcctgc agcccgcgcc agccggcatg caccgcggca
    10081 acctcgctgc cgtcacggtt gcacagcagt atcggcaggc aatcggcaac catcaccacg
    10141 gccacctggc cagggacacg ggtaatcgcg gcatcggcat cgcgccgcgt ctcggcttcg
    10201 agcaccaccg cgcgagtacc gtgggtttgc gcgatccagc tcggctcggc cggcaaatcg
    10261 agcgtacttc gcagcagcgc gcgattgcgc gccactcgcg cgggatcgtc gccgacatgc
    10321 aggccaagat tgaaaccggc gtaaggcggc tcgctgaaac ccccaccgcg ggtggtaaac
    10381 gccgagcgta ccgcgggcgg cgccgtccaa agaggttcga tgagctcaat catactgctc
    10441 gagtgcctcg agcaaatgct cgaaatctgc cgctaacggt gcttcgaaac tgactggctc
    10501 gcgggtcgcc ggatgcacga aggagagcct ctcggcatgc agcgcctggc gtctgaaagc
    10561 ggcaatccgc tcgctcagcg cggcgttcag gccggccggg atgcggttac gcccgccgta
    10621 aacgggatcg cccagcagcg ggtggtggag atgcgccagg tgcacgcgga tctggtgcgt
    10681 gcggccggtc tcgagctgca gcctgagcag gctgtaatgc gcgaacttgc gcactacctg
    10741 gaaatgggtc agcgccggct tgccgctttt ctgtacagac atttttttgc gctgctgcgg
    10801 atggcggccg atcgccagct cgatgctgcg cccggcagtc accacgccgc gcgcgatcgc
    10861 cacgtactcg cgctttacgc ggcgcgcctg cagttcctcc accagccagc gatgcgcgcg
    10921 caggttgcgc gccaccacca tgaccccggt ggtgtccttg tcgagccgat ggacgatacc
    10981 ggcgcgcggt agctgctcga ggcgcggatc atgagccagc aaaccgttca ccagtgtacc
    11041 gtcctggtgc cccgccgccg gatgcacgac cagcccggcg ggcttgtcga ctaccaggat
    11101 gtcgtcatcc tcgtgcagca ggtcaaattc gaccgcttgc ggcgggtcgg cgatacgcgc
    11161 ctgctcgggc acgtcgagat cgacgcgatc gccgggaaaa accttttgcc gcggtttgca
    11221 cagctgccgg ttgatggaga cgaaaccctg ctgaatccag ccctggataa ggctgcgcga
    11281 ataatccggc agcaattgct gtaatgcctg gtcgaggcga ctgc
//
LOCUS       DATCZV010000237         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_230740, whole genome shotgun sequence.
ACCESSION   DATCZV010000237 DATCZV010000000
VERSION     DATCZV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230740"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIW27_05285"
     CDS             <1..578
                     /locus_tag="VIW27_05285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5739115.1"
                     /translation="LLRHGMTAWNLEKRVQGRIDQPLCSAGRESLARLRLPCWAHECR
                     WYCSPLQRATQTAQLLGIEDFRIEEALLEMSWGDWEGEILKPLRRRLGDRMRHNESRG
                     LDFQPPGGESPRQVQARLRPWLRRVAATHRDSAAVVHKGVIRCIYSLARGWDMRGESP
                     VDFAWDALHRFELDVSGELADSYQAIPLEHA"
     gene            complement(664..1443)
                     /locus_tag="VIW27_05290"
     CDS             complement(664..1443)
                     /locus_tag="VIW27_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5739116.1"
                     /translation="MTRPSIAGQAWQWLERELERWSSDGRRALFWWRDDDASRAENRL
                     DRLLKLADKHALPLAIAAIPARLDDSLAGRLRDTRAISILQHGYAHDNFALPGQRSLE
                     LGGSRTVEEVAADLQRGFEIMGTAFGERYAAVLVPPWNRIDERLMPRLAGIGFRGIST
                     HKARRAAHPARGLLQVNTHLDPVDWRHADGFIGVYPAIAVLVQHLVARRAGYRDADEP
                     SGILSHHLAMNEATWKFLDQLLHFLNQHPAVEFVGANQIWN"
     gene            complement(1440..>2528)
                     /locus_tag="VIW27_05295"
     CDS             complement(1440..>2528)
                     /locus_tag="VIW27_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5739117.1"
                     /translation="ALLLAEALAAHGMRAEVVSGGLPPPMPVPAAVGFHQLAPARSPD
                     AAFTRLVDADGSALGDAWRVTRRRQLLDLFDRLEPHALITETFPFGRNLLRFELLPLL
                     EAARRSRSCRQTITSIRDILQPKTRPERNREICELLNRYYDQVLVHGDPSIAGLDYSF
                     DQYDTIRELVAYSGYITAASSAGAVEHTASGEVLVSAGGSPTGLKILETALAARPLTS
                     LAKRHWRIRVSHAIDAAQFAGLEKQAGGNTSIERNRDDFGQRMQRAALSVSQAGYNTV
                     CDLLASRAAAVVVPFAEADEAEQTLRAERLQARGRLVLLAQRDLDAQSLAAAIERALA
                     LDTRFEVDLDGARHSAALIARWLEESRR"
BASE COUNT          391 a          816 c          891 g          430 t
ORIGIN      
        1 tgctgctgcg ccacggaatg accgcctgga acctcgagaa gcgggtccag ggccgcatcg
       61 accagccgct ttgcagcgcc gggcgcgagt cgctcgcgcg gctgcggctg ccgtgctggg
      121 ctcacgaatg ccgctggtat tgcagtccgt tgcagcgcgc gacgcagacc gcgcaactgc
      181 tcggcatcga agattttcga atcgaggagg cgctgctcga aatgagctgg ggcgactggg
      241 agggcgagat attgaagccg ctgcgccgtc ggctcggtga tcgaatgcgg cacaacgaaa
      301 gccggggtct cgattttcag cctcccggcg gcgagagtcc gcggcaggta caggcgcggc
      361 tgcggccctg gctgcggcgc gtggcagcga cccaccgcga ctccgcggcg gtcgtgcaca
      421 agggcgtcat tcgctgtatt tactcgctgg cccgcggctg ggacatgcgc ggcgaatcgc
      481 cggtcgattt tgcctgggac gcgttgcatc gcttcgaact ggatgtcagc ggcgaactcg
      541 ccgacagcta ccaggccatt ccgctggagc atgcctgata ccggttgtac tggccgaggc
      601 cgtcggcctg gccgctagcc gggtgccgcg gccggttgcg cgccagccgg aaccgcggat
      661 cgatcaattc cagatttgat tcgcgccgac gaattcgacg gccgggtgct ggttcaggaa
      721 atgcagtaat tggtcgagaa atttccaggt cgcttcgttc atcgccaggt gatgactgag
      781 gattccgctg ggctcatcgg catcgcggta gccggcgcgc cgggcgacga ggtgttgcac
      841 gagcaccgcg atcgccgggt agacgccgat gaaaccgtcg gcgtgccgcc agtcgaccgg
      901 atcgaggtgg gtgttgacct gcagcagtcc gcgcgccggg tgcgccgcgc gccgtgcctt
      961 gtgggtcgag ataccacgaa atccgatgcc tgccaggcgg ggcatcaagc gctcgtcgat
     1021 gcggttccac ggcggtacca gtaccgctgc gtagcgctcg ccgaacgccg tacccatgat
     1081 ttcgaatccg cgttgcaggt cggcggcgac ctcttccacg gtgcgcgagc cgccgagttc
     1141 caggctgcgt tgaccgggca gggcgaagtt gtcgtgcgcg tatccgtgtt gcaggatgct
     1201 gatcgcccgc gtgtcgcgca gccgcccggc gaggctatcg tccaggcgtg ccggaatcgc
     1261 ggcgatggcc agcggcaggg cgtgtttgtc ggccaacttc agtagcctgt cgaggcggtt
     1321 ttcggcccgc gaggcgtcgt cgtcgcgcca ccagaaaagc gcgcgccgac cgtcgctgga
     1381 ccagcgctcc agttcgcgct ccagccactg ccaggcctgc ccggctatcg acggccgggt
     1441 catcgccgcg attcctctag ccagcgagcg atcagcgcgg cactgtggcg cgcgccgtcg
     1501 agatcgacct cgaagcgggt atcgagtgcc agcgcgcgct cgatcgccgc cgccagggat
     1561 tgcgcatcga gatcgcgttg tgccagcagg accagccggc cgcgagcctg cagccgctcg
     1621 gcgcgcaggg tctgctcggc ttcgtcggcc tcggcgaacg gcaccaccac cgcggcggcg
     1681 cggctggcga gcaggtcgca gacagtattg tagccggcct gcgacaccga cagcgcggct
     1741 cgttgcatgc gttggccgaa atcgtcgcga ttgcgttcga tgctggtgtt gccgccggcc
     1801 tgcttctcga ggccggcgaa ctgcgccgca tcgatggcat gactgacgcg aatccgccag
     1861 tgacgcttcg ccaacgaggt cagtggccgc gcggccagcg cggtctcgag aattttcagg
     1921 ccggtcggac tgccgccggc cgataccaac acttcgccgc tagccgtgtg ctcgacggcc
     1981 ccggcgctac tggcggccgt gatatagccg ctataggcga ccagttcgcg aatcgtgtcg
     2041 tattggtcga agctgtagtc gaggcctgcg atagacgggt cgccgtgcac cagcacttgg
     2101 tcgtaatagc ggtttaacag ttcgcagatt tcgcggttgc gttcggggcg cgtcttcggc
     2161 tgcaggatat cgcgaatcga cgtgatcgtc tgtcggcagc tgcggctgcg tcgagcggcc
     2221 tcgagcaacg gcagcaattc gaatcgcagc aggttcctgc caaacggaaa ggtttcggtg
     2281 attagcgcgt gaggttcgag ccggtcgaac aggtcgagca gctggcgccg gcgcgtaacc
     2341 cgccaggcat cgccgagcgc gctcccgtct gcatcgacga gccgtgtaaa ggcggcgtcc
     2401 gggctgcgcg ccggcgccag ctggtggaag ccgacagcgg ccggaaccgg catcggtggc
     2461 ggcaggccgc cggataccac ctcggcgcgc atgccgtgcg ccgcgagcgc ctccgccagc
     2521 aacagcgc
//
LOCUS       DATCZV010000238         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_707297, whole genome shotgun sequence.
ACCESSION   DATCZV010000238 DATCZV010000000
VERSION     DATCZV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707297"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /gene="pyrE"
                     /locus_tag="VIW27_05300"
     CDS             complement(<1..502)
                     /gene="pyrE"
                     /locus_tag="VIW27_05300"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY5739118.1"
                     /translation="MQDFQKRFVDYCLERGALKFGEFTLKSGRLSPYFFNAGLFNRGA
                     DLDALAAFYADAIVQSGIDFDLLFGPAYKGIPLAAITAGALYRNHDRDLPYAFNRKEA
                     KDHGEGGSTVGAEISGRVMIIDDVITAGTAIREAIALIEAHGARVTAITIALDRQERG
                     QGRLSAI"
     gene            577..1359
                     /locus_tag="VIW27_05305"
     CDS             577..1359
                     /locus_tag="VIW27_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY5739119.1"
                     /translation="MRVISINANGIRAAARKGFFDWLPRQRADIVCVQETKAQAEQLD
                     EPLYHPRNFHCSYYDALKKGYAGTAIYSRHRPLQVIRGFGESEFDNEGRYLEYRFPEL
                     SVISLYAPSGSSGDHRQESKWRFMDSFLLHLQALRRKRREFIICGDWNIAHQNIDLKN
                     WRSNQKNSGFLPEERAWLSDLFERVGYVDGFRVVDPRAEQYTWWSNRGQSWDKNVGWR
                     IDYQIVTPGLRDRIRKARIYRERRFSDHAPLIMDYDLELQTR"
     gene            1356..>2549
                     /locus_tag="VIW27_05310"
     CDS             1356..>2549
                     /locus_tag="VIW27_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739120.1"
                     /translation="MTNRSLPWLETFELFFHRRVVTMLFLGFSAGVPLLLIFSSLSLW
                     LREAGAERAAVTYFSWAALGYSFKFVWAPLVDQLPLPLLTRRLGRRRAWLLTAQAAIV
                     GAILCMAAIDPAASESSLTLMALAAVLLGFSSATQDIVIDAYRIESAEVDLQAMMSAT
                     YIAGYRIGMLVAGAGSLYLADWFGSSSEVYDYAAWQWTYRAMALAMGVGVITTLIIRE
                     PNTRVSGDDFRSSGDYVRFLMLFVVAIGGFAGLFFVSGDPVDLLKAHFVGAFGAPFLA
                     AFLMESLRLVTAIGFAWVLAILLVRSGLVDYGMVERSYLRPVRDFFERYGWSLAWILL
                     ALIGLYRISDIVLGVIANVFYQDLGFSKTEIASVVKTFGLLMTIAGGFVGGLLAARFG
                     VVRILL"
BASE COUNT          399 a          754 c          842 g          554 t
ORIGIN      
        1 ggatcgccga caggcgtccc tggccgcgtt cctggcggtc cagtgcgatg gtaatggcgg
       61 taacgcgagc gccgtgggcc tcgatcagcg cgatcgcttc gcggatcgcg gttccggcgg
      121 tgatcacgtc gtcgatgatc atcacccgcc cgctgatctc ggcgccgacc gtgctgccgc
      181 cttcgccgtg atccttggct tccttgcgat tgaacgcata gggaaggtcc cggtcgtggt
      241 tgcggtagag cgcgccggcg gtgatcgcgg ccagcggtat gcccttgtaa gcgggtccga
      301 acagcaggtc gaagtcgatc ccggactgca cgatcgcgtc ggcgtagaaa gcggccagcg
      361 cgtcgaggtc ggcgccgcgg ttgaagaggc cggcattgaa gaaatagggg ctcagtcgcc
      421 cggacttgag cgtgaactcg ccgaatttca gcgcgccgcg ctcgaggcag tagtcgacga
      481 aacgtttctg gaagtcttgc attcggggat tgccggctag tcgcttatct caagtcatgt
      541 atcatatcgc cagtttctct tattggcagc aataacatgc gggtgatctc aatcaacgcc
      601 aacggtattc gtgcggccgc gcgcaagggc tttttcgact ggctgccgcg gcagcgcgcc
      661 gatatcgtct gcgtgcagga aaccaaggca caggccgagc aactcgacga gccgctgtat
      721 cacccgagga attttcattg cagctactac gacgcactga agaaagggta cgcgggcacc
      781 gcgatctaca gccgccaccg gccgctacag gtgattcgcg gtttcggcga atccgagttc
      841 gacaatgaag gccgttacct cgaataccgc tttcccgagc tgtcggtgat ttcgctgtac
      901 gcgccgtccg gctcgtccgg cgatcaccgc caggagtcca agtggcgatt catggacagt
      961 ttcctgctgc acctgcaggc gctgcgccgc aagcggcgcg agttcatcat ctgcggcgac
     1021 tggaatatcg cgcaccagaa tatcgatttg aagaactggc gctcgaacca gaaaaattcg
     1081 ggtttcctgc cggaggagcg ggcctggctt tccgacctgt tcgagcgtgt cggctacgtc
     1141 gacgggttcc gcgtcgttga tccgcgcgcc gagcagtaca cctggtggtc caaccgcggc
     1201 cagtcctggg acaagaacgt cggctggcgc atcgactatc aaatcgtcac cccggggctt
     1261 agggacagga ttcgcaaggc gcgcatctac cgcgagcggc gcttttccga tcacgcgccg
     1321 ctgatcatgg attacgatct ggagctgcaa acccggtgac caaccgctcg ctgccgtggc
     1381 tggagacctt cgagctgttt ttccaccgcc gcgtggtgac gatgctgttt ctgggttttt
     1441 cggcgggcgt gccgctgctg ctgatctttt cgtcgctgtc gctgtggttg cgcgaggctg
     1501 gtgccgagcg cgcggcggtg acctatttca gctgggcagc tctcggttac tcgttcaagt
     1561 tcgtctgggc gccgctggtc gatcagctgc cgctgccgtt gctgacccgc cgcctcggcc
     1621 ggcgccgtgc ctggctgctc accgcgcagg cggcgatcgt tggcgcgatc ctgtgcatgg
     1681 cggcgatcga cccggcggcg agcgagtcga gcctgacgct gatggcgctg gccgcggtat
     1741 tgctcggctt ttcgtcggcg acccaggata tcgtcatcga cgcttaccgg atcgaatcgg
     1801 ccgaggtgga tttgcaggcg atgatgtcgg cgacctatat cgcgggttac cgtatcggca
     1861 tgctggtcgc cggcgccggc tcgctgtatc tcgccgactg gttcggcagc agcagcgagg
     1921 tttacgatta cgctgcctgg caatggactt accgggcgat ggcgctggca atgggggttg
     1981 gcgtgatcac cacgctgatc attcgcgagc cgaatacccg ggttagcggc gacgattttc
     2041 gctcgagtgg cgattacgtg agattcctga tgctgttcgt ggtcgccatc ggcggcttcg
     2101 ccggcctgtt ttttgtctcc ggagatccgg tcgacctgct caaggcccat ttcgtaggcg
     2161 cgttcggcgc tccgttcctg gcggctttcc tgatggagag cctgcgcctg gtgacggcga
     2221 tcggttttgc ctgggtgctg gcgatactgc tggtcaggtc ggggctggtc gactacggca
     2281 tggtggagcg cagctacctg aggccggtgc gggatttctt cgagcgctac ggctggtcac
     2341 tggcctggat tttactcgcg ctgatcggcc tttatcgaat ctcggatatc gtgctcggcg
     2401 taatcgccaa cgtgttttac caggatctcg gtttcagcaa gaccgaaatc gccagcgtgg
     2461 tcaagacctt cggcctgctg atgacgatcg ccggaggctt cgtcggcggg ctgctggcgg
     2521 cgcgcttcgg cgtggtcagg atcctgttg
//
LOCUS       DATCZV010000239         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_707434, whole genome shotgun sequence.
ACCESSION   DATCZV010000239 DATCZV010000000
VERSION     DATCZV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707434"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..819)
                     /locus_tag="VIW27_05315"
     CDS             complement(37..819)
                     /locus_tag="VIW27_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5739121.1"
                     /translation="MVSPERYLDGRVALVTGGFAGIGKAIALALAARGALLAVGARRQ
                     PASAIAELRAACPNLFHHPLDVADPGSVETFVAALEELHGPADILVNSAGVTAHQPVC
                     GHDERTWNQVIDINLSGPFRMIRACLPRMIERRWGRIINIGSTAATTATDDHAAYCAS
                     KSGLLGLNRAVALEGAPHGVSSVVISPTWVQTEMMDASMRTRARVAGIGVEQAYAEVI
                     AAQPQRRLVQPAELGALAAFLCRDEALGITMEDIQLNAGALW"
     gene            complement(813..1253)
                     /locus_tag="VIW27_05320"
     CDS             complement(813..1253)
                     /locus_tag="VIW27_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5739122.1"
                     /translation="MKYLHTMVRVHDLEASLGFYRDLLGLVETGRYESEEGQFTLVYL
                     AAPDDLERAESERAPLLELTYNWDGEVYQGGRNFGHLAFAVDDVYAICQKAMDAGITI
                     NVPPRDGHMAFFRSPDNISIELLQKGEALPPQEPWASMQTTGEW"
     gene            1359..>2385
                     /locus_tag="VIW27_05325"
     CDS             1359..>2385
                     /locus_tag="VIW27_05325"
                     /inference="COORDINATES: protein motif:HMM:NF005732.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEY5739123.1"
                     /translation="MNFNPHVLDCAAAPIAEAWSWVRDPASEQLIDMCQAVPAHLPPQ
                     SLRDYLGDAIKAGEGATYTDIRGIPPLREALAANISERYRGSVSADDVLITAGCNQAF
                     CSVIDVLCREGDEVIVPLPCYFNHQMWLSTRAVTPRYLSFNAGTGMPDPAEARGLINE
                     RTRAILLVTPNNPCGAIYTPARIAEFYDLAAEHGIALITDETYRDFIDIEQLPHALFA
                     RDDWRDTLVHLYSFSKVFSLTGFRTGAVVAGQALLEQLRKVQDCTAICAPHAGQLAAV
                     YGLQHLHDWKLEKSDELARRAVAIRQAFEHPQLEYRLITAGAYFAYVEHPFELPAREV
                     VKRLVQEQ"
BASE COUNT          442 a          800 c          738 g          405 t
ORIGIN      
        1 gctggctgac agattcaata tcgacatcag gccaccctac cataatgcac cggcattgag
       61 ctgaatatct tccatggtga tacccagcgc ctcgtcgcgg cacaggaatg ccgccagcgc
      121 gccgagttcg gccggctgca ccaggcgccg ctgcggctgc gcggcgatta cctcggcata
      181 ggcttgttcg acaccgatgc cggcaacgcg ggccctggtc cgcatgctgg cgtccatcat
      241 ctcggtctgc acccaggtcg ggctgatcac taccgagctg acgccatgcg gtgcgccttc
      301 cagcgcgacc gcgcggttca gtcccagcaa tcccgacttg gaggcacagt aggccgcatg
      361 gtcgtcggtc gcggtagtcg cagcggtgga accgatattg atgattcgcc cccagcgccg
      421 ctcgatcatt cgcggcagac aagcgcgaat catgcgaaac gggccggaca aattgatatc
      481 gatgacctgg ttccaggtgc gttcgtcgtg gccgcagaca ggctggtggg cggtgactcc
      541 ggcggagttg accaggatgt ccgccggtcc atgcaactct tcgagcgcgg cgacgaaagt
      601 ctcgacactg ccgggatcgg cgacgtcgag cgggtgatga aaaagattcg ggcaggccgc
      661 ccgcagctcg gcgatcgccg aggcgggctg acggcgcgcg ccgacggcaa gcagcgcgcc
      721 gcgcgcggcg agcgccagcg cgatcgcctt gccgataccg gcaaagccgc cggtgaccag
      781 cgcgacgcgg ccgtcgagat agcgctccgg gcttaccact cgccggtggt ttgcatcgac
      841 gcccagggtt cctgcggcgg cagcgcttca cccttttgca gcaactcgat cgaaatattg
      901 tccggcgagc ggaaaaacgc catgtgaccg tcgcgcggcg gcacattgat cgtgatgccg
      961 gcatccatgg ctttctggca gatcgcgtag acatcgtcca ccgcaaacgc gaggtgaccg
     1021 aagttacgac cgccctggta aacctcgcca tcccagttgt aggtcagctc gagcagcggc
     1081 gcgcgctcgg actcggcccg ctcgaggtca tccggcgctg ccaggtaaac cagcgtgaac
     1141 tgtccctcct cgctttcgta gcggccggtt tcgaccagcc ccagcaaatc gcggtaaaaa
     1201 ccgagcgatg cctccaggtc gtgcacgcgc accatggtat gcaggtattt cattgttact
     1261 cccgtattga atcagggcga cattatcgca cagccctgcc gggggtggta agccgcagcg
     1321 gaattcacta acatcgacgc aaactaacgg taagcgtcat gaattttaac ccgcatgtac
     1381 tcgattgtgc cgccgcgcca atcgccgagg cctggtcctg ggtcagggac ccggcatccg
     1441 aacagctgat cgacatgtgc caggcagtgc cggctcacct gccgccgcaa tcgctgcgcg
     1501 actacctcgg cgatgcgatc aaggccggcg aaggggccac ctataccgat attcgcggca
     1561 ttccgccgct gcgcgaggcg ctggctgcca acattagcga gcgctaccga ggctcggtca
     1621 gcgccgacga cgtgctgatc accgccggct gcaaccaggc cttttgctcg gtcatcgacg
     1681 tactgtgccg cgagggcgac gaagtcatcg tgccgctgcc gtgctacttc aaccaccaga
     1741 tgtggctgtc gacgcgtgcg gtgacgccgc gctacctgtc attcaacgcc gggaccggca
     1801 tgccggaccc cgccgaggcg cgcggcctga tcaacgagcg tacccgcgcg atcctgctgg
     1861 tgacgccgaa caatccctgc ggcgcgatct atacgcccgc gcggatcgcc gaattttacg
     1921 atctcgccgc cgaacacggc atcgcactga ttaccgacga gacttaccgc gacttcatcg
     1981 acatcgaaca gctgccgcac gctctgttcg cgcgcgacga ctggcgcgat acgctggttc
     2041 acctgtacag cttctccaag gtgttcagcc tcaccggatt tcgcaccggc gcggtggtcg
     2101 ccggccaggc cctgctcgag cagctgagaa aggtgcagga ctgcaccgcg atctgcgcgc
     2161 cgcacgccgg ccagctggcc gccgtttacg gcctgcaaca cctgcacgac tggaaactcg
     2221 aaaaatcgga cgagctggcg cggcgcgcgg tcgccatcag gcaagccttc gagcacccgc
     2281 agctggaata ccggctgata accgccgggg cttacttcgc ctacgtcgag caccccttcg
     2341 aactgcccgc gcgcgaggtc gtcaagcgcc tggtgcagga acagg
//
LOCUS       DATCZV010000240         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_71557, whole genome shotgun sequence.
ACCESSION   DATCZV010000240 DATCZV010000000
VERSION     DATCZV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71557"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VIW27_05330"
     CDS             complement(<1..1062)
                     /locus_tag="VIW27_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5739124.1"
                     /translation="MTVDELSGRLAVILHADVIGSTLLVQRDEQLAHRRIQEAFRRFG
                     KTISSYHGRVRELRGDALLAEFERASDAVAASLAFQREQARLVDALDDEIRPLLRIGV
                     ALGEVIVADNTVTGEGVVLAQRLEQFAEPGGLCITPAIREALPKRLPFALDNLGAREL
                     KGFDDPIDVYRVALQDGAVPPPPTAAPAAARTGKRRGPGIAVAAVLVSIAAAAIYALL
                     VPAPPPVDGLESADKPSIVVLPFTNMSGDPQQEYFADGISEDLTTDLSKISGLFVVAR
                     NSAFSYKGRQVDVRTVAEELGVRYLLEGSIRRVDDQIRINAQLVDGISGGHLWAERFD
                     GTMADVFALQDHVNREIVRA"
     gene            complement(1198..1935)
                     /locus_tag="VIW27_05335"
     CDS             complement(1198..1935)
                     /locus_tag="VIW27_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739125.1"
                     /translation="MKRRGFVKLCAAAVAGITAAAETIAGEQQQYRRYGRVALVDPDN
                     GRPVRAASLEVGETYVFHYPFISTPCFLVDLGKTVEARTELRTHDGAPYVWRGGAGPK
                     SSVVAFSAICAHKMTHPARAVSFINYRHGETRFRNSDDRVEQRAGVIYCCSENSVYDP
                     ARGGQVLGGPAPQPLAAIELDYRPGEDTFYATATIGGEMFDRFFGDFHDRLVLEHGRT
                     DIDRTMQGTSELVRLSDFTSNLVSCQA"
     gene            complement(2006..>2308)
                     /locus_tag="VIW27_05340"
     CDS             complement(2006..>2308)
                     /locus_tag="VIW27_05340"
                     /inference="COORDINATES: protein motif:HMM:NF028177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HEY5739126.1"
                     /translation="AYACLSGPDGLQRCEVPLAGYMLLAPDFEYVDHRHAPREIYLVL
                     TPGARWRLDGGEWFDVEPGELIVHHPWQLHAMRTGDRPLLAFAAWLEAGERRAIEI"
BASE COUNT          425 a          777 c          734 g          372 t
ORIGIN      
        1 agctcttaca atttcgcggt tgacatggtc ctgcagcgcg aacacgtcgg ccatcgtgcc
       61 gtcgaaacgc tcggcccaga ggtgcccgcc gctgatgccg tccaccagct gcgcattgat
      121 tcgaatctga tcgtcgacgc ggcgaatgct gccctccagc aggtagcgca cgccgagttc
      181 ctcggccacg gtacgcacat cgacctggcg gcccttgtag gaaaaggccg aattgcgcgc
      241 caccacgaac agtcccgaaa tcttcgacaa atccgtggtc aggtcctcgg agatgccgtc
      301 ggcaaaatac tcctgctgcg gatcgccgct catgttggtg aacggcaaga ccacgatcga
      361 tggcttgtcg gcggattcca gcccgtccac cggcggcggc gccggcacca gcagcgcata
      421 gattgcggcc gcggcgatcg acaccaggac cgccgccacc gcaatcccgg ggccgcgccg
      481 tttgccggta cgcgcggccg cgggcgccgc cgtcggcggc ggcggcacgg caccgtcctg
      541 caacgccacc cggtagacgt cgatcggatc gtcgaagcct ttcagttcgc gggcgccgag
      601 gttatcgagc gcaaacggca gccgcttcgg cagcgcctct cgaattgccg gcgtgatgca
      661 cagaccgccg ggttcggcga actgttcgag acgctgcgcc agcaccacgc cctcgccggt
      721 gacggtattg tcggcgacga tgacctcgcc gagcgcgacc ccgatgcgca gcagcggacg
      781 aatttcgtcg tcgagcgcgt cgaccagccg cgcctgttcg cgctgaaaag ccagtgacgc
      841 cgcgaccgcg tccgaggcgc gttcgaactc ggccagcagc gcgtcgccgc gcaactcgcg
      901 cacccgcccg tgataactcg agatggtctt gccgaaacgc cgaaaggcct cctgtatgcg
      961 ccggtgcgca agctgttcgt cgcgctgcac cagcagcgtc gaaccgatca cgtcggcgtg
     1021 caggatcacc gcgagtctgc cgctgagctc gtcaaccgtc atctaatttc cgcacccgga
     1081 tagcgaaaag cgacagtata ctccgtgcgc cacagaagtg attgtgccaa ccgccagtgc
     1141 aacagccgct caccacaggc cttgccccgg ccgcggcaat cggctggcaa ctgcgggcta
     1201 ggcctggcag ctgaccagat tgctggtgaa atcgctcagc cgcaccagtt ccgaggtccc
     1261 ctgcatcgtg cggtcgatgt cagtacgccc gtgctcgagt accagccggt cgtgaaaatc
     1321 gccgaaaaag cgatcgaaca tttcgccgcc gatagtcgcg gtggcgtaaa acgtgtcctc
     1381 gccgggccgg tagtcgagct cgattgcggc cagcggctgc ggcgccgggc cgcccagtac
     1441 ctggccaccg cgggccgggt cgtagacgct gttctccgaa cagcagtaga tgaccccggc
     1501 acgttgctcg acgcggtcgt cactgttgcg aaagcgggtt tcgccgtggc ggtaattgat
     1561 gaagctgacc gcgcgcgccg ggtgagtcat cttgtgcgcg cagatcgccg aaaatgccac
     1621 caccgaggat ttcggcccgg cgccgccgcg ccagacatag ggcgcaccgt cgtgggtgcg
     1681 caactcggta cgcgcctcga ccgtcttgcc gagatcgacc agaaaacagg gcgtggagat
     1741 gaacggatag tgaaacacat aggtttcgcc gacctctagc gacgcggctc tgaccggccg
     1801 gccattgtcg ggatcgacca gcgcgacgcg cccgtagcgg cggtactgct gttgttcacc
     1861 agcgatggtt tcggcagcgg cggtaatacc ggcaaccgcg gccgcgcaca gcttgacgaa
     1921 acctcttctc ttcatgcggg ttttctcccg gtagcctacg aaattataaa aagtatagag
     1981 taactccgct ggccaaggta gcccgtcata tctcgatcgc gcggcgctcg ccggcctcca
     2041 gccaggcggc gaaagccagc agcgggcggt cgccggtgcg catcgcgtgc agctgccagg
     2101 gatggtgaac gatcagttcg ccgggctcga catcgaacca ttcgccgccg tcgagacgcc
     2161 agcgcgcacc cggcgtcagt accaggtaaa tctcgcgcgg cgcatgccgg tgatcgacgt
     2221 actcgaagtc cggcgcgagc agcatatagc cggcgagcgg cacctcgcag cgctgcaggc
     2281 cgtcgggccc ggaaagacag gcataggc
//
LOCUS       DATCZV010000241         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_124623, whole genome shotgun sequence.
ACCESSION   DATCZV010000241 DATCZV010000000
VERSION     DATCZV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124623"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..811)
                     /locus_tag="VIW27_05345"
     CDS             complement(23..811)
                     /locus_tag="VIW27_05345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEY5739127.1"
                     /translation="MKPAELEAVFARIPQLAGRAPDEFTITPLPGYTNRNLRLHDGEQ
                     DWVLRVPRASTDRFVDREAEAHNQALACGLGIAPRPAWRDLSGLMLTPTLAASRNPAP
                     RDFTARPAAEALLEPVQRLHRSGAAFRGRVDLVELLHRYYLLLDAALRHSYRQRLRAA
                     QESLSRLDDRDLEYVPSHNDLVLENLLLEDGRVWLIDWEFSSLASPYWDLATLCNAAA
                     LDDAQSVALLQLYCAGGPQMEESLLFDYRNLLQLLSDCWMAALA"
     gene            complement(808..1515)
                     /locus_tag="VIW27_05350"
     CDS             complement(808..1515)
                     /locus_tag="VIW27_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5739128.1"
                     /translation="MLEIRRLEFAYSNAAPRMHFELDVEGGEVLSLIGPSGSGKSTLL
                     ALLAGFLEPSSGQILLDGEAIEGMPVERRPVSIVFQQHNLFPHLDIHTNVAIGIDPSL
                     RLLPEQARAVDEALERLGLGGLERRRPGELSGGQQQRVALARVLVRRRRILLLDEAFA
                     ALGPAQREEMIGLVRQLVAEQRMVALLVSHQPRDATLASARTAFVCDGRIAALQETRA
                     LLDESPLAEVRDYLGSL"
     gene            complement(1508..>2358)
                     /locus_tag="VIW27_05355"
     CDS             complement(1508..>2358)
                     /locus_tag="VIW27_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5739129.1"
                     /translation="ALCVSVALLTLRLQRLPEVEISIEPVAVVERRGLSLVNPLLIAL
                     AAVYVGMPLLSMLLDALSGPWLAVLRQASFWQAVANTLFIGLGAALLSVTAGWFLLRY
                     GASLAYDGRTQAARWIELAGSVVYVVPPLVIGTGMFVLLSSRVDVFDWVYPIVIAVNA
                     LMGLPFVIRSLAPPMRQNHARYQRLCRSLALGGWNRFRHLEWPLLRRPLGLAAGLVAA
                     LAMGDLGVIALFGTPDSATLSLLIYQQLGAYRVAEATVTALLLLALCLAAFWLLERGI
                     GGSGDA"
BASE COUNT          459 a          785 c          778 g          336 t
ORIGIN      
        1 ggtcagcatg cttgtcgccc tgtcaggcta gcgcggccat ccagcaatcg ctgagcaatt
       61 gcagcaggtt gcggtagtcg aaaagaagcg attcttccat ttgcggcccg ccggcgcaat
      121 acagttgcag cagtgcgacg ctttgtgcgt catcgagcgc ggccgcgttg cacagcgtcg
      181 ccaggtccca gtaaggcgag gccagcgacg agaactccca gtcgatcagc cacacgcgcc
      241 catcttcgag caacaggttt tcgagtacca ggtcgttgtg cgagggcacg tattcgagat
      301 cgcgatcatc gagccgcgac aaagattcct gcgccgctcg caggcgctgg cgatagctgt
      361 gccgcaacgc tgcgtcgagc agcaggtaat agcggtgcaa caattccacc aggtcgacgc
      421 ggccgcgaaa tgccgcaccg ctgcgatgca ggcgctgcac cggctccagc aaggcctcgg
      481 cggccggccg cgcggtgaag tctcgcggcg cgggattgcg gctcgcggcg agcgtcggcg
      541 tcagcatcag acccgacagg tcgcgccagg cgggccgcgg cgcgataccg aggccgcagg
      601 ccagcgcctg gttgtgcgct tcggcttcac ggtcgacgaa gcgatcggtg ctggctcgcg
      661 gcacacgcaa aacccagtcc tgctcgccgt cgtgcaggcg caggttgcga ttggtgtagc
      721 cgggcagcgg cgtgatcgtg aattcgtccg gcgcgcggcc ggcaagttgt ggaattcgcg
      781 caaacacggc ctcgagctcg gccggcttca cagcgagccc aggtagtcgc ggacctccgc
      841 cagcggcgat tcgtcgagca gtgcgcgggt ttcctgcagc gcggcgatgc gtccgtcaca
      901 aacgaaagcg gtgcgcgccg aggcaagcgt cgcatcgcgc ggctgatgac tgaccagcag
      961 cgcgaccatg cgctgttcgg cgacgagttg acggaccaga cctatcatct cttcgcgctg
     1021 cgccggaccg agggccgcga aggcctcgtc gagcaacagg attcggcggc gccgaaccag
     1081 cacgcgcgcc agcgccacgc gctgttgctg accgccggag agttcgcccg gcctgcggcg
     1141 ctcgagcccg ccgagaccga gccgctcaag ggcctcgtcg accgcccttg cctgctcggg
     1201 cagcagccgc agcgagggat cgataccgat tgccacgttg gtatgaatat cgagatgcgg
     1261 gaacaggttg tgctgctgga acacgatcga taccggccgc cgctccaccg gcatgccttc
     1321 gatggcttcg ccgtcgagca gaatttgccc gctcgagggc tcgaggaacc ccgccagtag
     1381 tgcgagcagc gtcgacttgc cggaaccact gggaccgatc aggctcaaga cttcgccacc
     1441 ctcgacatcg agctcgaaat gcatgcgcgg tgccgcgttc gagtaagcga attcgagccg
     1501 cctgatttca agcatcgccg ctaccgccga taccgcgctc gagcagccag aaagccgcca
     1561 ggcacagcgc cagcagcaac agcgcggtga ctgttgcctc cgctacccgg taggcgccga
     1621 gttgctggta gatcaacagg ctgagggttg ccgaatccgg cgtgccgaac agcgcgatga
     1681 cgccgagatc gcccattgcc agggccgcga ccagtccagc ggcaagcccc agcggccgcc
     1741 gcaacaacgg ccattcgagg tgacgaaaac ggttccagcc gccgagcgcg aggctacggc
     1801 aaaggcgctg gtagcgggca tggttctggc gcatcggcgg cgcgaggctg cgaatcacga
     1861 acggcaagcc catcaaggca ttgacggcga tgacgatcgg gtacacccag tcgaaaacgt
     1921 cgacccgcga tgacagcagc acgaacattc cggtgccaat taccagcggc ggcaccacgt
     1981 agacgaccga cccggcaagc tcgatccagc gggccgcctg cgtgcgcccg tcgtatgcga
     2041 gactggcgcc gtagcgcaac agaaaccagc cggcggtcac cgacagcagc gccgcgccga
     2101 ggccgatgaa cagggtattg gcgaccgcct gccagaagct cgcttggcgc aataccgcga
     2161 gccacgggcc ggagagcgcg tcgagcaaca tcgatagcag cggcataccg acgtagacgg
     2221 ccgccagagc gatcagcagc gggtttacca gcgacagacc acggcgctcg acgaccgcca
     2281 ccggctcgat cgaaatttcg acctcgggca ggcgctgcag cctcagcgtc agcaaagcca
     2341 cactcacgca tagcgcga
//
LOCUS       DATCZV010000242         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_19507, whole genome shotgun sequence.
ACCESSION   DATCZV010000242 DATCZV010000000
VERSION     DATCZV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19507"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VIW27_05360"
     CDS             <1..894
                     /locus_tag="VIW27_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739130.1"
                     /translation="ENIRVSHRSLARSLDLHPEVFVSAYWADMVPHHIPDDAAYCRKL
                     RIQVDKVIAERRTIRGHFHVGMGERVGSFFQQRGVDLAKLLIGALDIKDEVMTYFLRE
                     TELYDRDQYIADRMRAPLEAALRDAWNAGMEPVVIAHSMGSFIAYDVLWRFSHRRDSG
                     FAAYNRKRVKMFVSLGSPLGDTSVRNLLFARHHRQHGRRQYPTNIDSWHNYACLGDVV
                     AHQKNFHDIFYQPMRALQLFPADRKFRTIDYVNLHNPFEVVSHAGNRGREKRNPHKSY
                     GYLAQPRLGSWLADYLLDRLL"
     gene            complement(915..2477)
                     /locus_tag="VIW27_05365"
     CDS             complement(915..2477)
                     /locus_tag="VIW27_05365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5739131.1"
                     /translation="MGNASRAGEQVEEGGKRRFTIYADFNCPFSYALNERLHAMGLDQ
                     RVDFRCIRQFPSAASDATDFQTLGLLAGEVAELRRKLPSMQINLPLFRPDTTAAADLA
                     NAVITVDPARAARLRRHIFRALWIDGEDISRPELLRSLLRDLEIDLPGKSIRRAPDTD
                     DWQRKWDENPDFQQDTPIVISDRGETLVGLPLESELDVFLESGSMVSDRAPLVGQRLN
                     GRQRILLLDDDLDCLRMLIEQLPEAQIEVAADFPGLVASTVNNGVPDLVLANMELLGG
                     VDNVDWWRDSIDSELDALLPVIFISDDAGTEAEVAAFEAGAADFLARPLHPRVLRARI
                     NTHLRARRTQQQLKNIARIDALTSICNRREFDLRLMSEWGRGARSEHSLALLMIDVDH
                     FKQYNDTYGHLRGDDCLVKVAQVLSGCLQRAGDLIARYGGEEFVILLSGVELEGATRV
                     AEACRAAIVDARLTHDSSSVSPWVTVSVGVAAMRPIYDKSCTLLVEEADVALYQAKQD
                     GRNRVSQFAAEG"
     gene            2985..>3504
                     /locus_tag="VIW27_05370"
     CDS             2985..>3504
                     /locus_tag="VIW27_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739132.1"
                     /translation="MVSDTPRWGEALGFPRYQVSEQLLDDGSVRVIGNVTLAGLRLEW
                     EEPPANWIAPHWFEQYRLFTRGPIRGMTLAASIEERGEHSGLEMTLSFETANPLGSLL
                     AKRLVAAFEDKVRSLLDTAERLLAAERPELFASNYRPPPAALRRGQQLAGEIAASPYG
                     HGLAERLLEYVNE"
BASE COUNT          645 a         1172 c         1083 g          604 t
ORIGIN      
        1 gagaacattc gcgtcagtca ccgttcactg gcgcgcagtc tcgacctgca cccggaagtc
       61 ttcgtctccg cctactgggc cgacatggtg ccgcaccaca ttcctgacga cgcggcctat
      121 tgccgcaagc tgcgcatcca ggtcgacaag gtgatcgccg agcggcgcac gattcgcggc
      181 cactttcacg tcggtatggg cgaacgggtc ggtagttttt tccagcagcg cggcgtcgac
      241 ctggccaagc tgttgatcgg cgcgctcgac atcaaggacg aggttatgac ttacttcctg
      301 cgcgaaaccg agctctacga tcgcgaccag tatatcgccg accgcatgcg cgcgccgctg
      361 gaggccgcgc tgcgtgatgc ctggaacgcg ggcatggaac cggtagtgat cgcgcacagc
      421 atgggcagct tcatcgccta cgacgtgtta tggcgctttt cccaccgccg cgacagcggc
      481 ttcgcagcct acaaccgcaa gcgggtaaaa atgttcgtca gcctcggttc gccgctcggc
      541 gatacatcgg ttcgcaacct gctgttcgca cgccaccacc gccagcacgg caggcgccag
      601 tacccgacca atatcgacag ctggcacaac tacgcctgcc tcggcgacgt ggttgcgcat
      661 caaaagaatt ttcacgatat tttctaccag ccgatgcgcg cgctgcagct gttcccggcc
      721 gacaggaaat tccgcaccat cgattacgtc aacctgcaca atccgttcga ggtcgtcagc
      781 cacgccggca accgcggccg cgaaaaacgc aatccgcaca agtcatacgg ctacctcgcg
      841 cagccgcggc tcggcagctg gctcgcggac tacctgctgg atcggttgct gtaacagggg
      901 tcgccggccg gcgctcagcc ctcggccgcg aactggctga cgcggttacg cccgtcctgc
      961 ttggcctggt aaagggcgac gtcggcctcc tcgaccaaca gtgtgcagct cttgtcgtaa
     1021 atcgggcgca tcgcggcgac gccgacgctg accgtcaccc acggcgagac gctagacgaa
     1081 tcgtgtgtca ggcgggcatc gacgatcgcc gcacgacagg cctcggcgac gcgcgtcgcg
     1141 ccctcgagtt cgacacccga cagcaggatc acgaattcct caccaccata gcgcgcgatc
     1201 aggtcgccgg cacgctgcag gcagccgctg aggacctgcg ctaccttgac caggcaatcg
     1261 tcaccgcgca aatgcccgta ggtgtcgttg tattgcttga aatggtcgac gtcgatcatc
     1321 agcaacgcca gcgagtgctc ggagcgcgcg ccacggcccc attcggacat caaccgcagg
     1381 tcgaactcgc gccggttgca gatcgaggtc aacgcatcga tccgcgcaat attcttcagc
     1441 tgctgctgcg tgcgccgcgc gcgcaggtgg gtattgatgc gcgcgcgcaa tacccggggg
     1501 tgtagcggcc gggcgagaaa atcggcggca ccggcctcga acgcggcgac ttcggcctcg
     1561 gtgccggcgt cgtcggagat gaaaataacc ggcagcaggg cgtcgagttc ggagtcgata
     1621 gagtcgcgcc accagtcgac attgtcgacc ccgccgagca gttccatgtt ggccagcacc
     1681 aggtcgggta cgccattatt gaccgtcgag gccaccagac ccggaaaatc cgcggcgacc
     1741 tcgatctgcg cctccggaag ttgctcgatc agcattcgca ggcaatccag gtcgtcatcc
     1801 agcagcagga ttcgctggcg gccgttcagc cgctggccga cgagcggcgc gcggtccgat
     1861 accatcgaac cggattcgag aaacacgtcc agctcggatt ccagcggcag gccgaccagg
     1921 gtctcgccgc gatcgctgat gacgatcggc gtgtcctgct ggaaatccgg gttttcgtcc
     1981 cacttgcgct gccaatcatc ggtgtccggc gcgcgtcgga tcgatttgcc gggcaggtcg
     2041 atttcgagat ccctgagcag cgaacgcagc agctcgggac gcgagatatc ttcaccgtcg
     2101 atccacagcg ccctgaatat atgccgccgc aggcgcgcgg cccgcgcggg gtccacggtg
     2161 attacggcat tggcaagatc cgccgcggcg gtcgtgtcgg ggcgaaacag cggcaggttg
     2221 atctgcattg acggcagctt gcggcgcagt tcggcgacct cgcccgccag caggccaagg
     2281 gtctgaaaat cggttgcgtc gctggccgcc gacgggaatt gccgaataca gcggaaatcg
     2341 acacgctgat cgagacccat cgcgtgtaga cgctcattta gcgcgtaact aaaagggcag
     2401 ttgaaatcgg cgtagatcgt gaatcgtcgc ttgccgcctt cttctacctg ctcccctgcg
     2461 cgtgaagcat ttcccatgcc ggggttcccg ttgctatcgt tgtattatta tcggcgactc
     2521 agtcgctcgt ccataagctt ccattatagg ccattacgcg gtgcaaccga agcgcgaatt
     2581 gaggcgcgcg tcggcggctg gccggcgcgt cggcggcgtg ataaggtctt tggcggattc
     2641 gcggcgccag cagccggcag tccaacgacc gcgatgcaat cctggcaact gccgatcgcg
     2701 gggccactaa ccgccgctcg cgggcccgcg cacaaggctg ccacagccac ggctccgggt
     2761 ttcaaagccg cgcgccggca accgatattc gcgtcaagcc ggagcgccat cgatgccggc
     2821 cggggcgacg gccgaatgat cgaaaatcgg aatcgccgcg tataaccagt cgcgcgtccg
     2881 gcaggttccg cggccgccga accctggatt acggagacag ccagctgagc gtttcacgca
     2941 cctatcgatt cgatttcgcc taccccgcag agcgcctgtg ggaagtggtt tccgacaccc
     3001 cgcgctgggg agaagcgctc ggttttcccc gctaccaggt cagcgaacag ctgctcgacg
     3061 acggcagtgt ccgggtgatc ggcaatgtca ccctcgccgg tctgcgcctc gaatgggaag
     3121 agccgcccgc caactggatc gcgccccact ggttcgaaca gtaccggctg ttcacccgcg
     3181 ggccgatccg cggcatgacg ctggccgcca gcatcgaaga gcgcggcgag cacagcggcc
     3241 tcgaaatgac actcagcttc gagaccgcca acccactcgg ctcgctgctc gccaagcgtc
     3301 tggtcgcggc cttcgaggac aaggtgcgtt cgctgctgga caccgccgag cgcctgctgg
     3361 cggccgagcg cccggaatta tttgccagca attatcggcc gccgcccgcg gcccttcggc
     3421 gcggccagca gctcgccggg gaaatcgccg cctcacctta cggtcacggc ctcgccgaac
     3481 gcctgctcga gtacgtcaac gagg
//
LOCUS       DATCZV010000243         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_178705, whole genome shotgun sequence.
ACCESSION   DATCZV010000243 DATCZV010000000
VERSION     DATCZV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178705"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VIW27_05375"
     CDS             complement(<1..700)
                     /locus_tag="VIW27_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739133.1"
                     /translation="MLDADKLLNCLPFTTLVDELAAMHREPIGLVDEMLMESADARDN
                     VNHFFIRSGWQPEKAIGTKVISIFPRNNQTQQYPSIQAVYVLFEGEHGSPLACLDGTA
                     LTWLKTAADSALGSSLLARDDVESMLMIGAGQMAPYLVAAHCALRPSITRVRIWNRSV
                     DKAARLCEQLVERLPQVAFDVSDDLESGVRGAELVCSAIGCIEPLVRGDWLQPGTHVD
                     LVGAYRPDMREADDT"
     gene            819..1547
                     /locus_tag="VIW27_05380"
     CDS             819..1547
                     /locus_tag="VIW27_05380"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5739134.1"
                     /translation="MSADRNQLGQPIGFPLPDWSGCEHPRGACMQGRRCRLEPLDPEL
                     HAGSLFSAYAEDRDGRNWTYLAYGPFADEAAFRGWLESACLGEDPCFFAVIDAADDRA
                     VGLASYLRIEPGSGVIEIGHLHFSPRMQGRPIATEAIYLMMRQVFDVLGYRRCEWKCD
                     ALNRPSRAAAERLGFAFEGIFRQATIYKGRNRDTAWYAIIDREWPAARGAFEAWLDAA
                     NFDDQGRQKSSLSKTMREALAPLR"
     gene            complement(1544..2218)
                     /locus_tag="VIW27_05385"
     CDS             complement(1544..2218)
                     /locus_tag="VIW27_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEY5739135.1"
                     /translation="MNTRDLVKTYVNPLFTRDYWQMLGEYLGIGKARKYRPIADRDSL
                     SEFIGSRASHVSQASLYGYLRTRAGTRFPAMFESPDLLVSINIAKWHIWLACLSDISI
                     YCGQCLRQSGELDGKTVGEMMTAAVQRVLDETGEPDEAGPDFAAARDKALQRVATCDW
                     SIDRDDDTVFSQSPDALFYWSPIADELKERDEPIVRNSIRYRWIEVRRNLRQLLDSKA
                     LAESLD"
     gene            2429..2686
                     /locus_tag="VIW27_05390"
     CDS             2429..2686
                     /locus_tag="VIW27_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEY5739136.1"
                     /translation="MDTSRREEHWRRTKSLMFVTLTIWFIFAFVVHWFANGLNSVNFF
                     GWPLGFYMAAQGSEIIFVITLFWFVKAQDKIDRDCGVAEED"
     gene            2690..>3092
                     /locus_tag="VIW27_05395"
     CDS             2690..>3092
                     /locus_tag="VIW27_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY5739137.1"
                     /translation="MAKLTSGGSFIENLPKIYGLYTGGFLVFVLLMAILEQMGVGADT
                     IGILFVAFTIAIYAGIGVLSRTMAVDAYYVAGRQVPAVFNGMATAADWMSGASFVAMA
                     GGIYFGGHGYLAFVVGWTGGYILVASLMAPYL"
BASE COUNT          563 a          911 c          972 g          646 t
ORIGIN      
        1 cggtatcgtc ggcctcgcgc atatccggcc ggtaggcgcc gaccaggtcg acatgcgtgc
       61 cgggctgcag ccagtcgccg cgaaccagcg gttcgatgca gccgatcgcc gagcacacca
      121 gttcggcccc gcgcacgccg ctctcgagat cgtcgctgac gtcaaatgcg acttgcggca
      181 ggcgttcgac gagctgttcg cacagccgtg cggccttgtc gacgctgcga ttccagatcc
      241 tgacccgcgt tatcgacggg cgcagcgcgc aatgcgcggc caccaggtag ggcgccattt
      301 gtccggcgcc gatcatcagc atcgactcga catcgtcgcg cgccagcagg ctgctgccga
      361 gcgccgagtc ggcggcggtc ttcagccagg tcagcgcggt gccgtcgagg caggcgagcg
      421 gcgagccgtg ctcgccctcg aaaagcacgt agaccgcctg gatcgacggg tattgctgcg
      481 tttgattgtt gcgcggaaag atggaaataa ccttggtgcc gatcgctttt tccggctgcc
      541 agccgctgcg aatgaaaaag tggttgacgt tgtcccgtgc atccgccgac tccatcagca
      601 tctcgtcgac cagcccgatc ggttcgcgat gcatcgccgc cagctcgtcg acgagggtcg
      661 tgaacggcag gcagttcaat aatttgtcgg catcgagcat cagcacgcgg tctttccccg
      721 gcgcctcgac aggcgctaga ctgtgtaaac tgttcagggt ggtggagtgt actcaaatcg
      781 cgccgccatt aacaatgcct atctggagga gatttcaatt gtcggcagat cgtaatcagc
      841 tggggcagcc gatcggcttt ccgctgccgg actggagtgg ctgcgagcat ccgcgtgggg
      901 cgtgcatgca ggggcgccgg tgccgcctgg aaccgctcga cccggaattg cacgccggct
      961 cgcttttctc ggcttatgcc gaggaccgtg acgggcgcaa ctggacttac ctggcttacg
     1021 ggccgttcgc cgacgaggcg gcgttccgcg gctggctgga atccgcctgc ctgggcgaag
     1081 atccgtgttt tttcgcggtg atcgatgccg ccgatgaccg cgccgtcgga ctcgccagtt
     1141 acctgcgtat cgagcccggc agcggtgtga tcgagatcgg gcatctgcat ttttcgccgc
     1201 gcatgcaggg caggccgatt gccaccgaag ccatctacct gatgatgcgc caggtattcg
     1261 acgtccttgg gtaccggcgt tgcgagtgga agtgcgatgc attgaatcgg ccatcgcgcg
     1321 cggcggccga gcggctgggt ttcgcgttcg aggggatttt tcgccaggcg acgatctaca
     1381 aggggcgcaa ccgcgatacc gcgtggtacg cgattatcga tcgcgagtgg ccggcggcga
     1441 ggggcgcctt cgaggcctgg ctcgatgccg ctaacttcga tgatcagggg cgccagaaat
     1501 ccagcctgtc gaagaccatg cgcgaggctc tcgccccgct gcgctagtcg agtgattcgg
     1561 cgagtgcctt gctgtcgagc agctggcgca ggttacggcg tacttctatc catcgataac
     1621 gaatcgaatt cctgacgatc ggttcgtcgc gctccttcag ttcgtcggcg atcggcgacc
     1681 agtaaaacag tgcatccggg ctctggctga agacggtatc gtcgtcgcgg tcgatactcc
     1741 agtcgcaggt agcgacgcgc tgcaaggcct tgtcgcgtgc cgccgcgaaa tccgggccgg
     1801 cttcgtcggg ctcgccggtc tcgtcgagca cccgctgcac cgctgcggtc atcatctcgc
     1861 cgacggtttt gccgtcgagt tcccccgact gccgcaggca ctgcccgcag tagatgctga
     1921 tgtcggacag gcaggccagc cagatgtgcc atttggcgat attgatcgat accagtagat
     1981 cggggctttc gaacatcgcc ggaaagcgcg tgccggcgcg cgtgcgcagg tagccgtaca
     2041 gcgacgcctg cgacacgtgg ctggcgcggc tgccgatgaa ttcgctcagc gaatcgcggt
     2101 cggcgatagg ccggtatttg cgggccttgc cgataccgag gtattcaccg agcatttgcc
     2161 agtaatcccg tgtgaatagt gggttgacat aagttttgac cagatcgcgg gtattcacgg
     2221 ttaatccgat taatttgtta ccaaacgtaa caagccatga tgagaaacag aataatctgc
     2281 accaaaatcg attcgatggg ctctaatact agggttttcg tctggcaaat tccacgaatt
     2341 ctgtgcatat taccctcgcg ataaaaaata aatattacgc gcaaccaaac gttaacgata
     2401 accaacccaa tataggggga cttaaccgat ggatacttcc agacgagagg aacactggcg
     2461 gcgaaccaag tcgctgatgt tcgtgactct cacgatttgg ttcatctttg cttttgtggt
     2521 gcactggttc gccaacggcc ttaactcggt aaacttcttc ggatggccgc tgggtttcta
     2581 catggccgcc caaggttccg agatcatctt cgtgatcacc ctgttctggt tcgtcaaggc
     2641 ccaggacaag atcgatcgcg attgcggcgt ggccgaggag gactagacca tggcaaaact
     2701 aacaagcggc ggcagtttta tcgaaaacct gccgaaaatc tatggcctgt ataccggggg
     2761 attcctggta tttgtcctgt taatggcgat cctggagcag atgggcgtcg gtgccgacac
     2821 catcggcatc ctgttcgtgg ccttcaccat cgcgatctac gccggtatcg gggtactgtc
     2881 gagaaccatg gcggtcgacg cttattacgt cgccgggcgc caggtgccgg cggtgttcaa
     2941 cggcatggcg accgcggccg actggatgtc gggcgcctcg ttcgtggcga tggcgggcgg
     3001 catctacttc ggcggacacg gctacctcgc gttcgtcgtc ggctggaccg gcggttacat
     3061 cctggtcgcg tcgctgatgg cgccgtacct gc
//
LOCUS       DATCZV010000244         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_654944, whole genome shotgun sequence.
ACCESSION   DATCZV010000244 DATCZV010000000
VERSION     DATCZV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654944"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VIW27_05400"
     CDS             <1..1190
                     /locus_tag="VIW27_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5739138.1"
                     /translation="TPRNDIAILQLEDCSGIPEAVLGDSDLVRPGDETFAVGNPLGRN
                     PDSISRGIISHAQRYRDSTTPYLQTDAAINPGNSGGALFDRNGQVIGINTAIDSTRFG
                     TNLGVGFAVPINQVMQTVADLRQGPPSWGDAGFSGIVTTLTPDEAEVLQVPGGGGALV
                     VTRTPETGPSQGRLEAHDVIYRINETAIRDTAQAVRLVGQYGVGDVLSLDLIRGGEPK
                     EVEVVLGEGWQGDSVPAPDNYDGFLGMTLEMWGEAEGDRGQFKQPVITKVHSLGPAHR
                     AHVASSQRSVIVNGPYVIPYLLDVKIVTGVAYQGELHAITSVEEIEDFARRAYHDGEP
                     LLLEIEYWARSNPQDHKGDLELASTAFFKILPQRAILKKAVLASSRSSFEITKRHASS
                     RNI"
     gene            complement(1215..>2106)
                     /locus_tag="VIW27_05405"
     CDS             complement(1215..>2106)
                     /locus_tag="VIW27_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5739139.1"
                     /translation="LSQSEFEAKPWCYTVINTNSPRQLDIPMCQGIIDFARAGQVSVI
                     TPFTLAGAMAPVTMPGALVLQHAEALAGILLSQIVRPGAPVVYGSFTSNVDMKSGSPA
                     FGTPEFVQAAFGAGQLARHIGLPWRGSAATASNAPDEQAVYEFLMSAWGSILGGVNVM
                     VHAAGWLEGGLTGSMEKFILDVEMLQTFAEIFNPLDSGDAELAYEAIASVDPGGHFFG
                     CEHTMARYQTAFYEPLVSDWSNFGQWTENGSKTATQRANGIWKKILHDFEVPAMDPAR
                     LEELQAFIERRSAEGGAPVV"
BASE COUNT          412 a          645 c          655 g          394 t
ORIGIN      
        1 ccacccccag aaacgacatc gccattctgc agctcgagga ttgcagcggt attcccgagg
       61 ccgtgctcgg cgactccgac ctggtgcgac cgggggacga aaccttcgcg gtcggaaatc
      121 ccctggggcg caatcccgat tcgataagtc gtggcatcat ttcgcatgcc cagcgctacc
      181 gcgatagtac gacaccctac ctgcaaaccg acgccgcgat aaacccgggt aattcgggcg
      241 gcgcgctgtt cgatcgtaac ggccaggtta tcggaatcaa cacggcaatc gattcgaccc
      301 ggttcggcac taacctcggg gttggtttcg cggtgccgat caaccaggtt atgcagacag
      361 tcgccgattt gcgccagggc ccgccgagct ggggtgacgc gggcttctcc ggtatcgtca
      421 ccacgctgac gccggacgaa gccgaggtgc tgcaggtgcc gggcggcggt ggcgcgctgg
      481 tcgttaccag aacgcccgag acgggtccca gccagggcag gctggaggcg catgacgtta
      541 tctaccgtat caacgaaacc gcgatcaggg acaccgcgca ggcggtgcgc ctggttggcc
      601 agtacggggt tggtgatgtc ctgtcgctcg acctgatacg tggcggagag ccaaaggaag
      661 tcgaggtcgt gctgggcgaa ggctggcagg gcgatagcgt cccggcgccg gataattatg
      721 acggttttct cggcatgacc ctggagatgt ggggcgaagc ggagggcgat cgtggccagt
      781 tcaagcaacc ggttatcacc aaggttcaca gcctgggtcc ggcacaccgg gcccacgtag
      841 cgagttcgca acgctcggtc atcgtcaatg gcccctatgt cattccctac ctgctcgacg
      901 tcaaaatcgt gaccggcgtg gcttatcagg gcgagttaca tgcgattacc tcggtggagg
      961 agatcgaaga cttcgcccgt cgcgcctatc atgacgggga gccgctgctg ctcgagatcg
     1021 agtactgggc acgcagcaat ccgcaagacc acaagggtga cctggaactg gccagcaccg
     1081 cttttttcaa aatcctgccg cagcgcgcga ttttgaaaaa agcggtgctg gccagttcca
     1141 ggtcgtcgtt cgagattacc aagaggcacg ccagttccag gaatatctga tctgggcttt
     1201 gtgccgccag ccggttagac caccggcgcg ccgccctcgg cgctacggcg ttcgataaat
     1261 gcctgcaact cttcgagacg ggcgggatcc atcgccggca cttcgaagtc atgcaaaatc
     1321 tttttccaga tgccgttggc gcgctgggtc gcggtcttgc tgccgttttc ggtccattgt
     1381 ccaaaattac tccagtcgga aaccagcggt tcgtaaaacg cggtctggta gcgagccatg
     1441 gtgtgttcgc aaccgaaaaa atgaccgccc ggatcgacgc tggcaatcgc ctcgtaggcc
     1501 agttccgcgt cgccgctgtc gagcggattg aatatttcgg cgaaggtttg cagcatttcg
     1561 acgtcgagaa tgaacttttc catcgacccg gtgagcccgc cctccagcca gccggccgca
     1621 tgcaccatta cgttgacccc gccgagaata ctgccccagg ccgacatcag gaactcgtac
     1681 accgcctgct cgtccggcgc attcgatgcg gtcgccgccg agccgcgcca gggaaggccg
     1741 atatgccgtg ccagttgccc cgcaccgaac gccgcctgca cgaattcagg ggtgccgaag
     1801 gcaggtgaac ccgatttcat gtcgacgttc gaggtgaagg agccgtagac taccggcgcg
     1861 ccagggcgca cgatctgtga cagcaggatg ccggccagtg cctcggcgtg ctgcagcacc
     1921 agcgctcccg gcatggtcac cggcgccatc gcgccagcca gcgtaaacgg tgtaatcacc
     1981 gatacctgcc cggcgcgggc gaagtcgata atgccctggc acatcggaat atcgagctgg
     2041 cgcggcgagt tggtgttgat caccgtgtaa caccagggtt ttgcctcgaa ttccgactgg
     2101 cttaat
//
LOCUS       DATCZV010000245         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_654989, whole genome shotgun sequence.
ACCESSION   DATCZV010000245 DATCZV010000000
VERSION     DATCZV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654989"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VIW27_05410"
     CDS             complement(<1..1750)
                     /locus_tag="VIW27_05410"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5739140.1"
                     /translation="MFDEFDEWLCKTNFSFLHGASHPRELVQRADQLGYGSLCINDFD
                     GSYGLARAWRELDYLRKHGDYRGLQLNYGAEIHLHRDHQLPLLLQRTLVLVATDARGY
                     TNLNRLLSQAHRDSKHDAWLALDTLLGSDVDGLFAIQPMRGAIRRGDTGDNHGELGEL
                     FAGRYYLAISRHLHTAEDRWIALTLRQARRHGLQYILSQDVFFHQRAQKCVSDLLQAI
                     RCNRRLDDCLEHYFPNAERCLHRGDELARLFAPIPGASHALGLARELRQSCVFDLGQL
                     SYRYPREMIPAGENALSWLRQLVARGADEKFAGRPSRKVRRQLEHELELIERLDFADY
                     FLTVWDIVSWARDQGILCQGRGSAANSSVCYVLGITSVNPDQFDLLFERFVSMERGDP
                     PDIDVDFEHERREEVIQYIYRRYGRERAAMVANVVTFRGKGALRAVGKALGADDELLR
                     QMSKVASSRFYRGAGTGNIVMALKDDYQQRHPESPRIDWSLWVELAERLHGFPRHLGI
                     HSGGFMLADRPLDCLLPQEPATMAGRSVIQWSKEDIEALGFFKIDLLSLGMLSALRKC
                     FDYLRHYYGQTLSLDAI"
     gene            complement(1764..>2039)
                     /locus_tag="VIW27_05415"
     CDS             complement(1764..>2039)
                     /locus_tag="VIW27_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739141.1"
                     /translation="PGDPRQQNFDVYAWFCGAVSKPLFFYSTPEPIDNPQGMRKIFLE
                     RSSEQWWQHGNVLQAMRDYYILTDRNGRSIWAFRTRDGAWFKQGEYH"
BASE COUNT          400 a          629 c          641 g          369 t
ORIGIN      
        1 gtatggcgtc gagactcagc gtctgtccgt agtaatggcg caggtaatcg aaacacttgc
       61 gcagcgcaga cagcataccg aggctcaaca ggtcgatctt gaagaagccg agcgcctcga
      121 tgtcttcctt gctccactgg atcacgctgc gcccggccat ggttgccggt tcctgtggca
      181 acaggcaatc gagcggccgg tcggcgagca tgaagccgcc ggagtgaata ccgaggtgac
      241 gcggaaagcc gtgcagccgc tcggcgagct cgacccacag cgaccagtcg atgcgcggcg
      301 actccgggtg tcgctgctgg taatcgtctt tcagcgccat gacgatattg ccggtaccgg
      361 caccgcgata aaaacggctg gaagcgactt tcgacatttg ccgcagcaat tcgtcgtcgg
      421 cgccgagcgc cttgccgacc gcgcgcagcg cgcccttgcc gcgaaaggtc accacattgg
      481 cgaccatcgc cgcgcgctca cgaccatagc gccggtaaat gtactggatc acttcctcgc
      541 ggcgttcgtg ctcgaaatcg acgtcgatat cgggcggatc accgcgttcc atgctgacga
      601 agcgttcgaa tagcaagtcg aactggtcgg gattgaccga ggtgatgccg agcacgtagc
      661 agacgctgga gttggccgcc gaccccctgc cctgacagag tatgccctga tcgcgcgccc
      721 agctgacaat gtcccagacg gtcaggaaat aatcggcgaa atcgagccgc tcgatcagtt
      781 cgagttcgtg ctcgagttgc cggcgcacct tgcgcgaagg acggccggcg aacttttcgt
      841 cggcaccgcg cgccaccagc tggcgcagcc aggacagcgc attctcgccc gccgggatca
      901 tctccctggg gtagcgataa ctcaactggc cgaggtcgaa aacgcaggac tggcgcaact
      961 cgcgcgccag gccgagcgcg tggctggcgc ccggaatcgg cgcgaacagc cgcgcgagtt
     1021 cgtcgccgcg gtgcaggcag cgctcggcat tcggaaaata atgctcgagg cagtcgtcga
     1081 ggcggcgatt gcagcgtatc gcctgcagca ggtcgctgac gcatttctgc gcgcgctggt
     1141 ggaaaaacac gtcctggctg aggatgtact ggagaccgtg gcggcgcgcc tgccgcagcg
     1201 tgagcgcgat ccagcgatcc tcggccgtat gcagatgacg gctgatcgcg aggtaataac
     1261 ggccggcgaa cagctcgcca agttcgccgt gattatcgcc ggtatcgccg cggcgaatcg
     1321 cgccgcgcat cggctgtatc gcgaacaacc cgtcgacgtc gctgccgagc agtgtatcga
     1381 gcgccagcca cgcgtcgtgc ttgctgtcgc ggtgcgcctg gctcagcaaa cgattgagat
     1441 tggtataacc gcgcgcgtcg gtcgccacca gtaccagcgt gcgctgcagt aacagcggca
     1501 gctgatggtc cctgtgcaag tggatttcgg caccgtaatt gagctgcagg ccgcgatagt
     1561 caccgtgctt cctgaggtaa tcgagttcac gccaggcgcg cgccagaccg tagctgccgt
     1621 cgaaatcatt gatgcacagc gacccgtagc cgagttgatc ggcacgctgt accagttcgc
     1681 gcggatgcga ggcgccgtgc aggaagctga aattggtttt gcacagccat tcgtcgaatt
     1741 catcgaacat caggcggtcc acgctagtgg tattcaccct gcttgaacca ggcgccgtca
     1801 cgcgtccgga atgcccagat cgagcggccg ttacgatccg tcagaatgta gtaatcgcgc
     1861 atcgcctgca gcacattgcc atgctgccac cactgttccg agctgcgctc gagaaatatt
     1921 ttccgcatgc cttgcggatt gtcgatcggt tcgggtgtcg agtagaaaaa cagcggcttg
     1981 ctgacagcac cgcagaacca cgcgtaaaca tcgaaattct gctggcgtgg gtcgcccgg
//
LOCUS       DATCZV010000246         5198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_19974, whole genome shotgun sequence.
ACCESSION   DATCZV010000246 DATCZV010000000
VERSION     DATCZV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19974"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VIW27_05420"
     CDS             complement(<1..569)
                     /locus_tag="VIW27_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HEY5739142.1"
                     /translation="MAISVFDLFSIGIGPSSSHTVGPMRAAQQFAASLRDDGLLTRVA
                     RIKAELYGSLGATGKGHGSDKAVMLGLEGELPESIDTDSVDARLEAIRAAGKLHLCGE
                     QQLRFDEKSDLIMHRRQSLPYHSNGMTFRAHDAARESLREATFYSVGGGFIVDETAAG
                     ADRIVEDDTRLPFAFKSAVDLLIHCANHNL"
     gene            complement(667..3546)
                     /gene="gcvP"
                     /locus_tag="VIW27_05425"
     CDS             complement(667..3546)
                     /gene="gcvP"
                     /locus_tag="VIW27_05425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY5739143.1"
                     /translation="MPNLDLKLADLDIFHGFIRRHIGPDAEEEAAMLAELGMQSTDEL
                     LDKVVPESIRRRKPLEVKASVSERQVLLRLYEISIRNQLYKSFIGMGYYGTQTPSVIE
                     RNLLRNPAWYTSYTPYQAEISQGRLEALLNFQTMVSDLTGMELANASVLDEATAAAEA
                     MAMCHRLAKSKSDVFYISNSCHPQTIEVVTNRARHLKIKCVVGDPFDAADNPELFGVL
                     LQYPCSTGEVHDYRELVEKIHDKQALVTVAADLLSLTLLTPPGEFGADIVVGNSQRFG
                     VPLGYGGPHAAFMATRDAYKRSLPGRLIGVSRDSRGQPALRLTLQTREQHIRREKATS
                     NICTAQALLAIMASMYAVYHGPQEITRIAQRVHRLTVLLAEGLRELGLQVNTTHYFDT
                     ITVNTGNATEFIMAAARAKKMNLRQVDAHTVGISLDEVATRGDVARLYDVFTHDHGSD
                     VSIDDLDTRVGPALPPDLIRNSEFLGHPVFNSHHSETDLMRYMRKLQDKDLALDRTMI
                     PLGSCTMKLNSATELIPVTWPGFARIHPFVPLDQAQGYQQLIVELEQMLCACTGYDSI
                     SLQPNAGSQGEYAGLLAIKSYHESRDEGHRDVCLVPSSAHGTNPASAQMAGMKVVVVG
                     SAKNGNIDLDELRAKCEKHSDNLAAIMITYPSTHGVFEEDVTEVCDIVHQHGGQVYID
                     GANMNAMVGVCAPGKFGGDVSHLNLHKTFAIPHGGGGPGVGPIGVKVHLKPFLPSTSQ
                     KSNGGLNKVAAAPFGSAGILPISWAYISLLGRGGLPKATAIAILNANYIASRLKDHFP
                     ILYTGPNGLVAHECIIDTRVVKDFASVDDIAKRLIDYGFHAPTMSFPVAGTLMIEPTE
                     SESKEELDRFCDAMISIRNEIREIEEGRMDPDDNPLKNAPHTVDMICADEWGHAYQRE
                     QAAYPLSSLRDYKYWSPVGRIDNVYGDTHVFCSCPPLEDYAEEDA"
     gene            complement(3653..4438)
                     /locus_tag="VIW27_05430"
     CDS             complement(3653..4438)
                     /locus_tag="VIW27_05430"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY5739144.1"
                     /translation="MTRLRNLQLRFQDYLLGSSEDIERDIVSTENARAEHRLGAYYNA
                     YRIRLIDCLAIDYLALEKILGRTAFEELALDYLDHYPSTQPSVRWFGRHLPEYLHRVY
                     GGQDREFLADLANWEWALTTVFDAADSETLLALDDMARVAPEDWPRLRFEFKPALRWL
                     DLSWNAPPIRQACDAHREIPPQQRNQHPLRWLLWRRELKTHWRSLDVDEAWAFEQAAN
                     GADFAAICEGLLEWIDAEQVSLRAAGLLKQWVGDGLLLRVGKD"
     gene            complement(4435..>5198)
                     /locus_tag="VIW27_05435"
     CDS             complement(4435..>5198)
                     /locus_tag="VIW27_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY5739145.1"
                     /translation="PKNVDWFEIISENYMIDGGKPLYFLDAIRRDYPMVMHGVSMSLG
                     STDELNYDYLRQLKALIARVEPAWVSDHLCWTGVDHKNMHDLLPLPYTEESIGHVAER
                     ISRVQDFLGRQMLIENLSSYITYRDDAMPEWEFLSAIAERADCYLLLDVNNIYVSSFN
                     HRYDPLEYLAGVPAERVWQHHLAGHLNDGNLIIDTHDQDIIDPVWALYEETAKRFGPV
                     STMIERDGNIPELDGVLAELDHARRIAAPCYQSPP"
BASE COUNT          893 a         1515 c         1735 g         1055 t
ORIGIN      
        1 aggttgtggt tggcgcaatg gatcagcagg tcgacggcgg atttgaacgc gaagggcagc
       61 cgcgtgtcgt cctcgacgat gcgatccgca ccggcggctg tctcgtcgac gatgaagccg
      121 ccgccgaccg agtaaaaggt cgcctctcgc aggctctcgc gcgcggcgtc atgggcgcgg
      181 aacgtcatgc cgttggaatg gtaaggcaac gactgccggc ggtgcatgat gagatccgat
      241 ttttcgtcga aacgcagttg ctgttcgccg cagaggtgaa gcttgccggc ggcgcggatc
      301 gcttccaggc gcgcatcgac actgtcggtg tcgatgctct ccggcaattc gccttccagc
      361 ccgagcatta ccgccttgtc gctgccgtga cccttgccgg tggcgccgag cgagccgtag
      421 agttccgcct tgatgcgcgc gacgcgcgtt agcaggccgt cgtcgcgcag gcttgcggcg
      481 aattgctgcg cggcgcgcat ggggccgacg gtatgcgaac tcgagggtcc gataccgatc
      541 gaaaacaggt cgaatacgct gatcgccatc ggccaatcct atctctgcgg tgtgcgaatg
      601 tcgatcgcga gcatagacga acggcgcgtc gcgcgccgtc gctgaacgcg tcccgttatt
      661 gccagattac gcatcctcct cggcgtagtc ctcgagcggc gggcatgagc agaacacgtg
      721 ggtatcgccg tagacgttat cgatgcgtcc gaccggcgac cagtacttgt agtcgcgcag
      781 gctggacaac gggtaggcgg cctgttcacg ctgataggcg tgaccccatt catcggcgca
      841 gatcatgtcg acggtgtgcg gcgcgttctt caacgggttg tcgtcggggt ccatgcggcc
      901 ttcctcgatt tctcgaatct cgttgcggat cgagatcatc gcgtcgcaga agcggtcgag
      961 ctcttccttg gattccgact cggtgggctc gatcatcagt gtgccggcga ccgggaagga
     1021 catggtcggc gcgtgaaagc cgtagtcgat cagccgcttg gcgatgtcgt cgacgctggc
     1081 gaagtccttg accacgcggg tatcgatgat gcactcgtgc gcgaccagtc cgttggggcc
     1141 ggtatagagg atcggaaaat gatccttgag ccgactggcg atgtagttgg cgttgaggat
     1201 tgcgattgcc gtggctttcg gcaggccgcc gcgtccgagc agcgagatgt aagcccagct
     1261 gatcggcagg attccggcgc tgccgaaggg cgccgccgcg accttgttga gcccgccgtt
     1321 cgatttctgg ctggttgacg gcaggaaggg tttcagatgt accttgacgc cgatcgggcc
     1381 gactccgggc ccgccgccgc catgcggaat ggcgaaggtc ttgtgcaggt tcaggtgcga
     1441 tacgtcgccg ccgaacttgc ccggcgcgca gacgccgacc atcgcgttca tgttggcacc
     1501 gtcgatgtat acctgaccgc cgtgctggtg gacgatatcg cagacctcgg tgacgtcttc
     1561 ctcgaacacg ccgtgagtcg atggataggt gatcatgatc gcggcgaggt tgtcgctgtg
     1621 cttttcgcac ttggcgcgca gctcgtcgag gtcgatattg ccgtttttgg cgctgccgac
     1681 gacgaccact ttcataccgg ccatctgcgc cgaggccgga ttggtgccgt gggcagatga
     1741 cggcaccagg cagacgtcac gatggccttc atcgcggctc tcatggtagg acttgatcgc
     1801 cagcaagccg gcatactcgc cctgcgagcc ggcgttgggc tgcagcgaaa tcgagtcgta
     1861 accggtgcag gcgcacagca tctgttcgag ttcgacgatc agttgctggt agccctgcgc
     1921 ctggtcgagc ggcacgaacg gatggatacg ggcgaaaccg ggccaggtca ccgggatcag
     1981 ctcggtcgcc gagttcagct tcatggtgca ggagccgagc gggatcatcg tgcggtctag
     2041 cgccaggtcc ttgtcctgca gcttgcgcat gtagcgcatc aggtcggtct ccgagtggtg
     2101 gctgttgaat accgggtggc cgaggaattc gctgttgcga atcaggtccg gcggcagcgc
     2161 cgggccgacg cgggtgtcga gatcgtcgat cgatacgtcg ctgccgtgat cgtgggtgaa
     2221 tacgtcgtag aggcgggcca cgtcgccgcg tgttgcgacc tcgtcgagcg aaatgccgac
     2281 cgtgtgcgcg tcgacctgcc gcagattcat cttcttggcg cgtgcggcgg ccatgatgaa
     2341 ctcggttgca ttgccggtgt tgacggtaat ggtatcgaag taatgcgtgg tgttgacctg
     2401 gagaccgagc tcgcgcaggc cctcggcgag cagcacggtc aggcggtgca cgcgttgtgc
     2461 gatgcgggtg atttcctgcg gtccatggta gaccgcgtac atgctggcca tgatcgccag
     2521 cagtgcctgc gcggtgcaga tattgctggt cgccttttcg cggcgaatgt gctgctcgcg
     2581 ggtttgcagc gtaagcctga gcgccggctg gccgcggctg tccctggaga cgccgatcag
     2641 gcgcccgggc agcgagcgct tgtaggcatc gcgggtcgcc atgaacgccg cgtgcgggcc
     2701 gccgtagccg agcggcacgc cgaaacgttg cgaattgccg accacgatat cggcgccgaa
     2761 ttcgcccggc ggcgtcaaca gcgtcaggct caacagatcg gcggcaaccg tgaccagtgc
     2821 ctgcttgtcg tgaatctttt ccaccagctc gcggtaatcg tgaacctcgc cggtggagca
     2881 gggatactgc agcagcacgc cgaacagctc gggattgtcg gcggcgtcga acgggtcgcc
     2941 gacgacgcac ttgatcttca ggtggcgggc gcggttggtt accacctcga tggtttgcgg
     3001 gtggcagctg ttggagatat agaacacgtc gctcttgctc ttcgccagcc ggtggcacat
     3061 ggccatcgct tcggccgccg cggtggcctc gtcgagtacc gaggcattgg ccagttccat
     3121 gccggtcagg tcgctgacca tggtctggaa gttgagcagg gcttcgagcc gtccctggga
     3181 aatctccgcc tggtaaggtg tataggaggt ataccaggcc gggttgcgca gcagattgcg
     3241 ttcgatcacg ctcggcgtct gggtaccgta gtatcccatg ccgatgaaag acttgtagag
     3301 ctggttacga atcgagattt cgtacaaccg caacaggacc tggcgctcgc tgacgctggc
     3361 cttgacctcg agcggcttgc gccggcggat cgattcgggg acgaccttgt cgagcagttc
     3421 gtcggtgctc tgcataccga gttcggccag cattgccgct tcctcttcgg cgtcggggcc
     3481 gatgtggcgg cggatgaagc cgtggaagat atcgagatcc gcaagcttga ggtcgagatt
     3541 tggcatgatg attgtccttg gtcgctactc gcgggcaccg tacgccggtc ggcccgcact
     3601 gacgaaaagc cgttatatgg cgggaatctt aacactttag atagcgactg agttagtctt
     3661 tgccgaccct cagcagcagg ccgtcgccga cccactgctt caacagtccg gcggcacgca
     3721 gcgacacctg ttcggcgtcg atccattcca gtaatccctc gcagatcgcg gcgaaatcgg
     3781 caccgttggc ggcctgttcg aatgcccatg cctcgtcgac atcgagcgac cgccagtggg
     3841 tctttaattc ccggcgccac aacagccagc gcagcgggtg ctggtttcgc tgttgcggcg
     3901 ggatttcccg gtgcgcgtcg caagcctgcc ggattggcgg cgcattccag ctcaggtcca
     3961 gccagcgcaa cgcgggtttg aattcgaaac gcagccgcgg ccagtcctcg ggcgctaccc
     4021 gcgccatgtc gtctagcgcc aacagggttt cgctgtcggc ggcgtcgaat accgtcgtca
     4081 gcgcccactc ccagttggcc aggtcagcga ggaactcccg gtcctgaccg ccgtagacgc
     4141 ggtgcaggta ctccggcagg tgtcggccga accagcgcac cgagggctgc gtcgacgggt
     4201 aatggtcgag gtagtcgagc gccagttcct caaaggcggt gcggccaagt attttctcga
     4261 gcgccaaata atcgatcgcc aggcaatcga taagacgaat gcggtaggca ttgtaatagg
     4321 cgccgagccg gtgctcggcc cgcgcattct cggtactgac gatatcgcgc tcgatgtcct
     4381 ccgaactgcc cagcaggtaa tcctggaagc gcaactgcag gttacgcagg cgagtcatgg
     4441 tggcgactgg tagcagggag cggcgatgcg ccgcgcatgg tcgagctcgg cgagcacgcc
     4501 gtcgagttcg ggtatattgc cgtcgcgctc gatcatcgtc gataccgggc cgaagcgttt
     4561 tgcggtctcc tcgtacagcg cccagaccgg atcgatgatg tcctggtcgt gggtatcgat
     4621 gatcaggttg ccgtcgttga ggtggccggc aaggtggtgc tgccagacgc gctccgcggg
     4681 cactccagcg agatattcga gtgggtcgta gcgatggttg aagctgctga cgtagatatt
     4741 gttgacatcg agtaacaggt agcaatcggc gcgttcggcg atcgccgaga ggaattccca
     4801 ctccggcatc gcgtcgtcgc ggtaagtaat gtagctcgac aggttttcga tcaacatttg
     4861 tcggccgagg aaatcctgaa cccgtgaaat acgctcggcg acgtgaccaa tggactcctc
     4921 cgtgtagggc agcggcaaca ggtcgtgcat gttcttgtgg tcgacgccgg tccagcacag
     4981 gtggtcggat acccaggcgg gctcgacgcg cgcgatcagc gccttcaact gccgcaggta
     5041 gtcgtaattc agttcgtcgg tgctgcccag cgacatcgac accccgtgca tgaccatcgg
     5101 gtaatcgcgg cgaatcgcgt ccaggaagta aagcggcttg ccgccgtcga tcatatagtt
     5161 ttcgctgatg atttcgaacc agtcgacgtt tttcggct
//
LOCUS       DATCZV010000247         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_550211, whole genome shotgun sequence.
ACCESSION   DATCZV010000247 DATCZV010000000
VERSION     DATCZV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550211"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VIW27_05440"
     CDS             complement(<1..719)
                     /locus_tag="VIW27_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5739146.1"
                     /translation="MPRHEYSYDDLLAIQEIIRDAARHSLTVYYSCSTADYKTDGSIV
                     TEADMAMQSALTAALLERYPQVHMLGEEISEAEQNRVIDCEHDYWCLDPVDGTTNFHA
                     TVPLFSVSLGLISNGRIVLGVVYDPNRDECFGAVRGEGFWIDGKKVRRPSQPERLGDC
                     IAFVDFKRLPKDLSGSLVRDTPFKSQRNIGTCALEWAWLAAGRVNLLLHGRERIWDYA
                     AGVLLTEESGGHSETLDAEPV"
     gene            1052..>2394
                     /gene="sthA"
                     /locus_tag="VIW27_05445"
     CDS             1052..>2394
                     /gene="sthA"
                     /locus_tag="VIW27_05445"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEY5739147.1"
                     /translation="MGSKYDLIVIGSGPAGKRAAIQAAKIGKKVLMVEKNTIVGGVTV
                     HTGTIPSKTLRETVLFLSGWRQRGIYGLSYKVKEHITADDLKQRLMSTLNHEIEIIQH
                     QLMRNNVEIEYGHARFVDEHSISVEQQDGTKKQFSADHFVICVGTRTYRPDGIPFDGD
                     AIIDSDEILELKRIPRKMLVVGGGVIGIEYATIFKALDIDVTVLETNDFILGFVDREI
                     TDELIHYLRDCNIHVRLGDQVSTINRFDDGKVVVKFKSGKSMAVDLVLYAAGRQGVTE
                     DIGLEKVGIEADRRGRIPVNEDYQTALPHIYAAGDVIGFPSLASTSMEQGRHAACHAF
                     GAEVHSQPELFPYGIYAVPEISMVGLTEQECTERSIAYEVGIARFRETARGQIIGLRD
                     GMLKMLFSIETQKLLGVHIIGEGATELVHIGQAVISLGGTLDYFVQTVFNYPTLA"
BASE COUNT          494 a          699 c          738 g          463 t
ORIGIN      
        1 aagaccggtt ccgcatccag ggtttcgcta tgacctccgg attcctcggt cagcaatacc
       61 ccggcggcat agtcccagat gcgctcgcgc ccgtgcagca gcaagttgac gcgtccggcc
      121 gccagccagg cccactcgag cgcgcaggta ccgatgttgc gttgcgattt gaacggcgtg
      181 tcgcgcacca ggctgccgct gagatccttg ggcaggcgtt tgaaatcgac gaaggcaatg
      241 cagtcgccaa gccgctcggg ttgcgatggg cgccgcacct tcttgccgtc gatccagaag
      301 ccctcgccgc gaaccgcacc gaaacactcg tcgcggttgg ggtcgtaaac cacgccgagc
      361 acgatcctgc cattactgat taatcccagc gataccgaga acagcggtac cgtggcatga
      421 aaattggtgg tgccgtcgac cgggtcgagg caccagtagt cgtgctcgca gtcgatgacc
      481 cgattctgct cggcctcgct gatttcttcg ccgagcatat gcacctgcgg gtagcgttcg
      541 agcagcgcgg cggtcagcgc cgattgcatc gccatgtcgg cctcggtgac gattgagccg
      601 tcggtcttgt agtcggctgt ggaacagctg tagtaaaccg tgaggctgtg gcgggcggcg
      661 tcgcgaataa tctcctggat cgcgagcaga tcatcgtaac tatattcgtg gcggggcaac
      721 tacggctcca ggactcggaa ttgaacggat tcttttatct tgtcccagta taaacgacca
      781 acgccaaatt gcttaacatt tgccccggtg tctcgtcgag cgtcacggtt ttggaattgt
      841 ttggcggcac gcgcggcgtc atccggttac gctggtgcgc cgcccgggtg ccgggtccgg
      901 atttcagcat tcgcggcaga tggtctggtg ggcgcatagc gatacctgtg cacgatcgtc
      961 gacttcgcca ataccggggc gtagtaataa aattcggcga ctgcgctata aaaattagct
     1021 atattgggcg aaaaatcgag ggggaggagg tttgggctcg aaatacgatc tgatagtcat
     1081 cggcagcggc ccggcgggca agcgcgccgc gatccaggcg gcaaaaatcg gcaagaaggt
     1141 gctgatggtg gaaaagaata ccatcgtcgg cggcgtcacc gtgcataccg gcacgattcc
     1201 gagcaagacc ctgcgcgaaa ccgtgctgtt cctgtctggc tggcggcagc gcggcattta
     1261 tggcctcagt tacaaggtca aggaacacat caccgccgat gatctcaagc aacggctgat
     1321 gtcgacgcta aaccacgaga tcgaaatcat tcagcaccaa ctgatgcgca acaacgtcga
     1381 gatcgaatac ggtcatgcgc gcttcgtcga cgaacattcg atcagcgtcg agcagcagga
     1441 cggcacgaag aagcaattca gcgccgatca cttcgtgatt tgcgtcggta cccgtactta
     1501 ccggccggac ggtatcccgt tcgacggcga tgcgatcatc gacagcgacg aaatcctcga
     1561 gctgaaacgc ataccgcgca agatgctggt ggttggcggc ggtgtcatcg gcatcgagta
     1621 tgcgaccata ttcaaggcgc tcgacatcga cgtcaccgtg ctcgagacca acgatttcat
     1681 tctcggtttc gtcgatcgcg agatcaccga cgaactgatc cattacctgc gcgactgcaa
     1741 tatccacgtg cgtctcggcg accaggtcag caccatcaac cgtttcgacg atggcaaggt
     1801 ggtggtcaag ttcaagagcg gcaagagcat ggccgtggat ctcgtgctgt acgccgccgg
     1861 gcggcagggt gtgaccgagg atatcggact cgagaaggtc ggcatcgagg cggatcgccg
     1921 cggacggatt ccggtcaacg aggattacca gacggcgttg ccgcacatct atgcggccgg
     1981 cgacgtgatc ggctttccga gcctggcgtc gacgtcgatg gagcaggggc gccacgccgc
     2041 ctgccacgcc ttcggcgccg aggtgcacag ccagccggaa ctgtttccct acggtatcta
     2101 cgcggttccc gagatcagca tggtggggct caccgagcag gaatgcaccg agcgcagcat
     2161 cgcttacgag gtcggcatcg cgcgctttcg cgagaccgcg cgtggccaga tcatcggcct
     2221 gcgcgacggc atgctgaaga tgctgttttc gatcgaaacg caaaaactgc tcggcgtgca
     2281 catcatcggc gaaggcgcca ccgagctggt acacatcggc caggcggtga tttcgctcgg
     2341 cggcacgctc gattacttcg tgcaaacggt gttcaactac ccgacgctgg ccga
//
LOCUS       DATCZV010000248         6450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_655488, whole genome shotgun sequence.
ACCESSION   DATCZV010000248 DATCZV010000000
VERSION     DATCZV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655488"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VIW27_05450"
     CDS             complement(<1..611)
                     /locus_tag="VIW27_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009812493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5739148.1"
                     /translation="MSDKPETYRPIPGDQLPRFAGIATFMRLPYVAPADADEVDIGLI
                     GVPWDGGTTNRAGARHGPRQIRDLSTMARNVHHATGIAPFELCNCADLGDTPVNPVDI
                     EDTLGRVEDFFSRVVERGIVPLTAGGDHLATLPIMRALCRDEPVGMVHFDAHTDTLDR
                     YFGESKYTHGTPFRRAIEEGLLDPKRTVQIGIRGALYSAGDKD"
     gene            710..1687
                     /locus_tag="VIW27_05455"
     CDS             710..1687
                     /locus_tag="VIW27_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5739149.1"
                     /translation="MKRTPQFAPPPWLRNAHLQTILNSQGPRRLRARRALRRLAPQEL
                     ILRAADGTRLLAELDHASEERGALVVLLHGWEGSSRSAYMITTAATLLSAGYDVLRVN
                     LRDHGNTHHLNRELFNSTRSPEVAEALQTFLDEHRYRHAFLCGFSLGGSFALRIAADS
                     GQALGLGGAVAVCPPADPARAMGALNRGWFVYEQYFFKRWRASLRRKLECFPELQYGA
                     LLAAAKSIDDLNRDFIPRYTPYKQLDEYYAAYALTGSRLASLTVPAAIIAADDDPIIP
                     ADDFGRIDAHALLDIELQRFGGHCGFIENLAARSWIESRIIEILRRFAR"
     gene            complement(1713..2426)
                     /locus_tag="VIW27_05460"
     CDS             complement(1713..2426)
                     /locus_tag="VIW27_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5739150.1"
                     /translation="MNDWILTALALLTSTLTAITGIGGGMILIALMPGFMPAAAIVPV
                     HAMVQLFSNSSRALFGWRFLRWEYVLAFVAGGIAGGLVAAGIRREIDLEYTPLLIAAF
                     ILYSVWGPPLKLKHPPRGEFVVIGMLQTGLSMIVGATGPMAQAALLRRGLQRDALVVT
                     GALMTTFTHLIKVCLFALLGFSFLDYWQLIVGMSAGVISGAFIGTHIRYRIDEKIFRQ
                     ILKWALTLLALRMIYLTFA"
     gene            complement(2539..2730)
                     /locus_tag="VIW27_05465"
     CDS             complement(2539..2730)
                     /locus_tag="VIW27_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739151.1"
                     /translation="MFKINKAPEFKSLLSSPWGHSSTDNSYYGDPRYVKLEQYGLEHI
                     ERDRINDEGVEQVVILGYN"
     gene            complement(2832..3950)
                     /gene="dinB"
                     /locus_tag="VIW27_05470"
     CDS             complement(2832..3950)
                     /gene="dinB"
                     /locus_tag="VIW27_05470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY5739152.1"
                     /translation="MIPPPTAPRKIIHIDADCFYAAVEIRDNPALKGKPVAVGGSAAR
                     RGVIATASYEARRYGVRSAMASATAIKRCPQLILIPGRMSVYREASKQMQAIFADYTE
                     LIEPLSLDEAFLDVSGSELCRGSATLIAREIRQRIAAQIGITVSAGIAPNKFIAKVAS
                     DWNKPDGQHTVTPQQVDEFLETLPVKRIWGVGKVTAERLEQQGFRTCGDVRRCDIYRF
                     VQQFGQFGEHIHKLARGIDERAVQPEWRRKSVSVEHTYEQDLPDLDSCRAKLPALLEA
                     LLPRLQKLDPDYRIQHCFLKMKFHDFNQTTVERRETIPGYRDYAMLCEEAWQRGRIPV
                     RLLGIGARLVDLTDGSGQMDLFDGQDRDQSSVLQKTSH"
     gene            4015..4728
                     /locus_tag="VIW27_05475"
     CDS             4015..4728
                     /locus_tag="VIW27_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY5739153.1"
                     /translation="MTEKVFYQDSYLKTLQSEVVEIVDNGVILAATIFYPQGGGQPGD
                     RGRLTIGGREYPVSDTRYAEDRLRIVHVIDSADLGEIHAGDPVEMEIDWQRRHRLMRM
                     HTSMHLICSLIDAQATGGAVGETESRIDFDLQGQAIDKEQLTAELNRLVEQALPVSIG
                     AITDEELERNPGLVRTMSVQPPRGQGTVRTITIENTDYQPCGGTHVRNTAEIGKLLVT
                     GLKNKGKQNKRISMALVEP"
     gene            4822..6039
                     /locus_tag="VIW27_05480"
     CDS             4822..6039
                     /locus_tag="VIW27_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY5739154.1"
                     /translation="MTIRASIGLISLLMLLPPQLPADDSTGSCDHHIAQWLDPRSGKV
                     LQGEQLFERLAKANIVLLGEAHTTAAHHRWQAYMLGALHSRRNDMMVGFEMVPRRLQS
                     VLDDWTAGQLSEDDFLEQVEWEKVWGYDAGYYLPLFHFARLNRLPAIGLNVDRSLVSR
                     VAREGWESIDAADRDGLSDPAPASEAYREALARLYGYKQSLRAAEDEGGAGHGEIDLE
                     EIRNSDEFANFLAAQQTWDRGMAETLATAHRINPSALVVGIIGRGHLEYGYGIPHQLA
                     DLGIDGVEVLIPLNTKDTCDPLNPEFASAVFVVDAEDVAESPPGPRLGVFIENADSGV
                     RIKEIIEDSVAEASGLLADDIILRAADFETTTTAELIEVVKRQAPGTWLPLNIRRGDE
                     ELQLIAKFPQRFE"
     gene            6032..>6450
                     /locus_tag="VIW27_05485"
     CDS             6032..>6450
                     /locus_tag="VIW27_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739155.1"
                     /translation="MNSPVARLLAFLLLFACARAGAQAQVPQHDIDVRLDPASGEIEI
                     VDRLQVEGRDQYVFVLAPWLEIEKLAIDGSTAIPRRKGNHWSVSLPDRGRHRLEFSLS
                     GRLPARTPTASYAGASSGADGAYLPGYDSWIAIDPSRP"
BASE COUNT         1251 a         1947 c         2074 g         1178 t
ORIGIN      
        1 cagtccttgt cgccggcgct gtagagcgcg ccgcggatgc cgatttgtac ggtacgcttc
       61 ggatccagca agccctcttc gatagcgcgc cggaaaggcg tgccatgggt gtacttcgac
      121 tcgccgaaat agcggtcgag agtgtcggtg tgggcgtcga aatgcaccat gccgacgggt
      181 tcgtcgcggc acagcgcgcg catgatcggc agcgtggcga ggtgatcgcc accggcggta
      241 agcggcacta ttccccgttc gacgacgcgc gagaaaaaat cttcgacgcg gcccagggtg
      301 tcctcgatgt cgaccgggtt gaccggggta tcgccgaggt cggcgcagtt gcaaagttcg
      361 aatggtgcaa tgccggtggc gtggtggaca tttcgtgcca tcgtcgacag gtctcgaatc
      421 tgtcgtggac cgtggcgggc accggctcga ttggtggtgc cgccgtccca gggtacgccg
      481 atcaggccga tgtcgacttc gtcggcatcg gccggtgcca cgtacggcag gcgcatgaag
      541 gtagcaatac cggcgaagcg cggcagctgg tcgccgggaa tcggccggta agtctcgggt
      601 ttgtcgctca tctcggctct cggtaggcga taaagccaca ggctagattg acagtcgttt
      661 gactgcaacc ataatgtgcg gccatgagat tcgcagacaa ccggccgacg tgaagcgcac
      721 gccacaattc gcaccgccac cctggctgcg caatgcccac ctgcaaacga ttctcaacag
      781 tcagggtcca aggcggctgc gtgcccgacg cgcactgcgg cggctggcgc cgcaagaact
      841 gatcctgcgg gctgccgacg gcacgcgcct gctggcagag ctcgatcacg cgtccgaaga
      901 gcgtggcgcg ctggtggtcc tgctgcacgg ctgggagggc tcgagccgct ctgcctacat
      961 gatcaccacg gccgcgacac tgctgtcggc gggttatgac gtgctgcgcg tcaacctgcg
     1021 ggatcacggc aatacccacc acctcaatcg cgaactgttc aactcgacgc gctctcccga
     1081 ggtagcggaa gcgctgcaga ccttcctcga cgaacaccgt taccggcatg catttctttg
     1141 cgggttttct ctcggcggca gttttgcgtt gcgtatcgcc gccgacagtg ggcaggcgct
     1201 cggtctcggc ggtgcggtgg cggtttgccc gccggcggat ccggcgcgtg cgatgggtgc
     1261 gttgaaccgg ggctggttcg tctacgagca atattttttc aaacgctggc gagcgtcgct
     1321 gcgccgcaag ctggagtgct ttccggaact gcaatacggt gcactgctgg ccgcggcgaa
     1381 atcgatcgat gacctgaacc gcgatttcat accgcgatac acgccctaca agcagctcga
     1441 cgaatactac gccgcctatg cactgaccgg cagtcggctc gcgtcgctga ccgtgccggc
     1501 cgctatcatc gccgccgatg acgatccgat cattccggcc gacgacttcg gccggatcga
     1561 tgcccacgca ctgctggata tcgagttgca gcgcttcggc gggcattgcg ggttcatcga
     1621 aaacctggcg gcgcgcagtt ggatcgagag ccgtattatc gagattctgc ggcgcttcgc
     1681 gcgctgatcg tggcgcgcgg cgcgagcgat aatcaggcga aggtcagata aatcattcgc
     1741 agcgccagca gtgtcagcgc ccacttgagg atctgacgaa agattttttc atcgatgcgg
     1801 tagcgaatgt gggtgccgat aaaagccccg ctgatcaccc ccgcggacat gccgacgatg
     1861 agttgccagt agtcgaggaa cgaaaacccg agcaacgcga acaggcatac cttgatcagg
     1921 tgcgtgaagg tggtcatcag cgcgccggtc accaccaggg cgtcgcgctg caggccgcgg
     1981 cgcagcaatg ccgcctgtgc catcgggccg gtggcgccga ctatcatgct gagccctgtc
     2041 tgcagcatgc cgatcaccac gaattcgccg cgcggcggat gcttcagctt tagcggcggc
     2101 ccccagaccg agtacaggat gaaggcggcg attagcagcg gggtatactc gagatcgatt
     2161 tcgcgccgaa tgccggcagc gaccaggccg ccggcgatac cgccggcaac gaaagccagc
     2221 acgtactccc agcgcagaaa gcgccagccg aacagcgcgc ggctcgaatt ggagaacaac
     2281 tgtaccatcg catgcaccgg cacgatggct gccgcgggca tgaagccggg catcagtgcg
     2341 atcaggatca tgccgccacc gatgccggtg atcgcggtca gtgtcgaggt gagcagcgcg
     2401 agcgcggtca gtatccagtc attcatcgcg cgtgcgatgc ggcggaaaat cgcggcccag
     2461 cccggcccgc ggcgcaaacc ataaccgggc cgcgccgcgc gcggcgggga gggggtaaaa
     2521 gcccgctatt gccgcggctc agttgtagcc gagaatcacg acctgctcga cgccttcgtc
     2581 gttgatgcgg tcgcgttcga tatgctcgag tccgtactgc tccagcttca cgtagcgggg
     2641 atcgccgtaa tacgaattat ccgtcgacga atgaccccag ggcgaagaca gcagcgattt
     2701 gaactcggga gccttgttga tcttaaacat gtgttaccta accgtatacc agtaagttag
     2761 acgattaacc tatcaaccta catgtggtag tgaatataac ttactgataa gtaaaaacaa
     2821 gaaatacttt attaatgtga ggttttttgc aaaacgctcg attggtcgcg atcctggccg
     2881 tcgaacaggt ccatctgacc gctgccgtcg gtcagatcca ccagccgcgc gccgatgccg
     2941 agcaggcgca ccggtatgcg gccgcgttgc caggcttcct cgcaaagcat cgcgtagtcg
     3001 cgatagccgg gaatggtctc ccggcgttcg accgtggtct ggttgaaatc gtgaaatttc
     3061 attttcagaa aacagtgctg aatgcggtaa tccggatcga gtttttgcag gcgcggcagc
     3121 agggcttcga gcagcgccgg cagttttgcg cggcagctgt cgagatcggg cagatcctgc
     3181 tcgtaggtgt gttcgacgct gaccgatttg cgccgccact cgggctgtac cgcgcgttcg
     3241 tcgatgccgc gagcgagctt gtgaatatgc tcgccgaact gtccgaattg ctgcacgaag
     3301 cggtaaatat cgcagcggcg gacgtcgccg caggtgcgaa atccctgttg ctcgagccgc
     3361 tccgcggtga ccttgccgac tccccagatg cgtttgaccg gcagcgtctc gagaaactcg
     3421 tcgacctgtt gcggagtgac cgtgtgctgg ccgtcgggct tgttccagtc gctggccacc
     3481 ttggcgatga acttgtttgg cgcgataccc gcggaaaccg tgataccgat ctgggcggcg
     3541 atgcgctggc gaatttcacg cgcgatcagc gtggcgctgc cgcggcaaag ctccgatccg
     3601 gacacgtcga gaaatgcctc gtccagcgac agtggttcga tcaactcggt gtaatcggcg
     3661 aaaatcgcct gcatctgctt cgatgcttcg cggtagacgg acatgcgtcc cgggatcaat
     3721 atcaactgcg gacagcgctt gatcgcggtg gcgctggcca tcgccgagcg cacgccgtag
     3781 cggcgcgcct cgtagctcgc ggtcgcgatc acgccgcgcc gcgccgcgct gccgccgacc
     3841 gcaacaggtt tgcccttgag cgccgggttg tcgcgtatct cgaccgcggc gtaaaaacaa
     3901 tcggcgtcga tatggatgat cttgcgcggg gctgttggcg gcggtatcac ggcgatggct
     3961 tgcgtcgagg ctgcggctga gtaagaattt cgccatcaca ggcgaggcgc agcgatgacg
     4021 gaaaaagtat tttaccaaga cagttacctg aaaacactgc aaagcgaggt cgtcgaaatc
     4081 gtcgataacg gcgtgatcct cgcggcgacg attttttacc cgcagggcgg cggtcagccc
     4141 ggcgaccgcg ggcggctgac aatcggtggg cgcgaatacc cggtcagtga cacgcgctat
     4201 gccgaggatc gcctgcgcat cgttcacgtc atcgacagcg ccgatctcgg cgagattcac
     4261 gccggcgacc cggtggagat ggaaatcgac tggcagcgcc gccatcgcct gatgcgcatg
     4321 cataccagca tgcacctgat ctgctcgctg atcgatgcgc aggcgaccgg cggcgcggtc
     4381 ggcgaaaccg agagccgcat cgacttcgat ctgcagggcc aggcgatcga caaggagcag
     4441 ctcaccgcgg aactcaatcg gctggtcgag caggcgctgc cggtcagcat cggcgcgatc
     4501 accgacgagg aactcgagcg caatccgggg ctggtgcgaa ccatgtcggt gcagccgccg
     4561 cgcggccagg ggacggtgcg tacgattacc atcgagaata ccgactacca gccctgcggt
     4621 ggcacccacg tgcgcaatac cgccgagatc ggcaagctgc tggtgaccgg gctgaagaac
     4681 aagggcaagc agaacaagcg catcagcatg gcgctggtcg agccctgatt cgcacccgtc
     4741 ttcgataatg cagtttaatc cgggcgatgt aatcgtccgt atggtaactt tgtcaccgaa
     4801 tttccccgga gaagcaaacg catgacgatt cgtgcaagca ttggcctgat aagcctgttg
     4861 atgttgttgc cgccgcagct gccagccgac gacagcactg gcagctgcga tcaccacatc
     4921 gctcaatggc tcgatccgcg cagcggcaag gtgctgcagg gcgagcagtt attcgagcgc
     4981 ctggcgaagg cgaacatcgt tttgcttggc gaagcgcaca ccaccgcagc gcatcatcgc
     5041 tggcaggcat acatgctcgg cgccctgcat tcccgccgca acgacatgat ggtcggcttc
     5101 gaaatggtac cgcggcggct gcagtcggta ctcgatgact ggactgccgg ccaactgagc
     5161 gaggacgatt ttctcgaaca ggtggagtgg gaaaaggtct ggggttacga tgccggctat
     5221 tacctgccgc tgtttcattt cgcgcgtcta aaccgcctgc cggcgatcgg tctgaacgtc
     5281 gaccgcagcc tggtgtcgcg ggtagctcgc gagggctggg agtcgatcga cgccgccgac
     5341 cgtgacggcc tgtccgaccc ggcaccggcc agcgaggcct atcgtgaagc gctggccaga
     5401 ctctacggct acaagcaaag cctgcgtgcc gccgaagacg agggcggggc gggccacggg
     5461 gaaatcgatc tcgaggaaat tcgcaacagc gacgaattcg ccaatttcct cgccgcccag
     5521 cagacctggg atcgaggcat ggcggagacg ctggctactg cgcatcgaat caacccgtcg
     5581 gcgctggtgg tcggtatcat cggccgcggg cacctcgagt atgggtatgg aataccgcat
     5641 caactcgccg acctgggtat cgacggcgtc gaggtgctga taccgctgaa cacgaaggat
     5701 acctgcgatc cgctgaaccc ggagttcgcc tccgcggtgt tcgtcgtcga tgccgaagac
     5761 gttgccgaat caccgcccgg accgaggctg ggcgtgttca tcgaaaacgc cgacagcggc
     5821 gtgcgcatca aggaaatcat cgaggatagc gtggccgagg cgagtggcct gctggcggac
     5881 gatatcatcc tgcgcgcggc cgacttcgaa acgacgacga cggcggaatt gatcgaggtc
     5941 gtcaagcggc aggccccggg aacctggttg ccgttgaata ttcgacgcgg cgacgaggag
     6001 ttgcagctaa tagcgaaatt cccgcagcgc tttgaatagt ccggtcgcga gactccttgc
     6061 ctttttactg ctgttcgcct gtgcccgggc gggcgcgcag gcgcaggtgc cgcagcatga
     6121 tattgacgtc aggctggacc ccgccagcgg cgaaatcgaa atcgtcgacc ggctgcaggt
     6181 cgaggggcgc gaccagtatg tatttgtact cgcgccgtgg ctcgaaatcg aaaaactggc
     6241 gatcgacggc agcaccgcga taccacgcag aaagggcaat cactggagcg tctcgctgcc
     6301 cgatcgaggt cggcaccggc tcgaattttc gctgtccggc cggctgcctg cgcgaactcc
     6361 gacggcatct tatgcaggcg ccagtagtgg cgccgacggc gcttacctgc cgggctacga
     6421 cagctggatc gccatcgatc catcccgccc
//
LOCUS       DATCZV010000249         3575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_761428, whole genome shotgun sequence.
ACCESSION   DATCZV010000249 DATCZV010000000
VERSION     DATCZV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761428"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VIW27_05490"
     CDS             <1..1251
                     /locus_tag="VIW27_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5739156.1"
                     /translation="LPLKPRMSVNSIDRLVLDDDWSDLARLREMPQIDFERMARYRMA
                     RIKQQLELHDAELVVLVNPISLRYAVNFRSYLLFQSHIPQIYVFVPREGPVVAYGCYY
                     NLPQVTEFRPGRPHAFFDGGNNIDAAARDLADDVVEYLAEIGSSNRRVAVEYVNPSVT
                     QALEARGIEVIDGVRISEMARVIKSEDEIACMRWAIEVGQHGARMMKNALRPGISELQ
                     LWGLLNYTNIANNGDWHEGRMLASGPRINPWLQEASERKVESGDLVGFDTDMVGPFGY
                     FVDLSRTLHCGPAKPTRRQKQIYRMAVDEIEHNLKLVRPGMTLFEMQDQAYSIPEECR
                     DNAYVCMMHAVGMCDEYPQVRQGFRQRNAYDCTLEAGMVVCIESYMGPKGERDGVKIE
                     QQVLITEHGYQPLTTFPYEESLLD"
     gene            1357..1887
                     /locus_tag="VIW27_05495"
     CDS             1357..1887
                     /locus_tag="VIW27_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY5739157.1"
                     /translation="MTAFDLDTLVAELRAAAAGDGPARAVRKLMTDAFRDPVAIASAM
                     PEFENDDEVLFEDDRVSIWFVRFAPGIHVPPHDHRTTATIGVYDGAEINHFYLREPDR
                     LVHKSTRRVAAGEVIALKPDGIHSVEAADGKPSLGLHVYLAALTGIERSLYDWHSGAP
                     VPFTDQAFESMTRRSG"
     gene            2006..3403
                     /locus_tag="VIW27_05500"
     CDS             2006..3403
                     /locus_tag="VIW27_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739158.1"
                     /translation="MTRTTSKLLDEVEAFCRRAGIAETTFGREAVNDGKLCARLRAGK
                     SLTLETADRIRDYIASHGGDGKATTDDDAPVEPLGGRQQLETRHLTDDRPFRFYDNRQ
                     KYLAFVNTCNEKWKVGERATQELSHIHPVPPALRLFDAGVGDGTVLGHLMRAMHRRFP
                     TIPFFIVGKEISLEDVRLTLEKLPDRFIEHPSSVIIITNLYYAEAPWLMPNDVAKAAA
                     LNWIDMPLQGDSAYEFGEQLRGIDKELVDGWQVKASEKSGNPMYVRPSVLVMYREDHR
                     FLLDDVIPRRGRAAGGDYDLVLASQPWRARMDAEFKVRNVLGPLVRSLRAGGRVLTVQ
                     SSGDDPGLELIRRIWPDEDPFRVNRHELIKVLKEHLGRSAQNYNFNVGSDAKSIFRYR
                     MHTLPTEIGDAIGTSTLFAAWNAAIYVGQIEDERLEPVVSSGAYLDATADILHQHNGI
                     WFNDETFVVSRKRDA"
BASE COUNT          701 a         1104 c         1123 g          647 t
ORIGIN      
        1 ctaccactga agccgagaat gagtgtcaat tcgatcgacc ggctggtcct cgacgacgac
       61 tggagcgatc tcgcgcgact gcgggaaatg ccgcaaatcg atttcgagcg catggcgcgt
      121 tatcgcatgg cgcgcatcaa acagcagctc gaactgcacg acgccgaact ggtcgtgctg
      181 gtcaacccga tcagcctgcg ttatgcggtg aatttccgca gctatctgct gttccagtcg
      241 catattccgc agatttacgt gttcgtacca cgcgaaggcc cggtggtggc ctacggttgt
      301 tattacaatt tgccccaggt aacggagttt cgcccgggac gcccgcatgc ttttttcgat
      361 ggcggcaaca atatcgatgc ggcggcgcgc gatctcgccg acgacgtggt cgaatacctc
      421 gccgagatcg gcagcagtaa ccgccgggtc gcggtggagt acgtcaatcc gtccgtgacc
      481 caggcgctgg aggcgcgcgg catcgaggtc atcgatggtg tgcgcatcag cgaaatggcg
      541 cgggtgatca agtccgagga cgagatcgcc tgcatgcgct gggcgatcga ggtcggccag
      601 cacggcgcgc ggatgatgaa gaatgcgctg cgtcccggga tcagcgaact gcagctttgg
      661 ggtctgttga attacaccaa tatcgccaac aacggtgact ggcacgaggg ccgcatgctc
      721 gcttccggtc cgcgcatcaa tccctggctg caggaagcca gcgaacgcaa agtcgagtcc
      781 ggcgacctgg tcggcttcga taccgacatg gtcgggccgt tcgggtactt cgtcgacctg
      841 tcgcgcaccc tgcactgcgg tccggcaaaa ccgacgcgcc ggcaaaagca gatctaccgc
      901 atggcggtcg acgagatcga gcacaacctg aaactggtgc gccccggcat gacgctgttc
      961 gaaatgcagg accaggcata ttcgattccg gaggaatgcc gcgacaacgc ctatgtctgc
     1021 atgatgcacg cggtcggcat gtgtgacgag tacccgcagg tcaggcaggg ctttcgccag
     1081 cgcaacgcct atgactgcac gctcgaggcc ggcatggtgg tctgtatcga gagttacatg
     1141 ggtccgaagg gcgagcgcga cggcgtcaag atcgagcagc aggtgctgat caccgaacac
     1201 ggctaccagc cactgacgac ttttccctac gaggagtcct tactcgattg acgtcgcttc
     1261 gaatcgcggc gtaaaccagt gttctggtca ttcccactgc gccaagtcat tcccgcgcaa
     1321 gcgggaatct tgtagcattg gttaactgga aaagccatga ctgcattcga tctcgatacc
     1381 ttggtagccg agctgcgcgc agcggccgcg ggcgatgggc cagcgcgcgc ggtgcgcaag
     1441 ctgatgaccg acgcgtttcg cgatccggtg gcgatcgcgt cggcgatgcc ggagttcgaa
     1501 aatgacgatg aagtcctgtt cgaggacgac cgcgtttcga tctggttcgt gcgtttcgcc
     1561 cccgggattc acgtgccgcc ccacgaccat cggacgaccg cgaccatcgg cgtatacgac
     1621 ggtgccgaaa tcaatcactt ctacctgcgc gagcccgatc ggctggtgca caaatcgacc
     1681 cgccgcgtgg ccgccggcga agtgatcgcg ctgaaacccg acggcattca ctcggtcgag
     1741 gccgccgatg gcaaacccag tctcggcctt cacgtttacc tggcggccct gactggcatt
     1801 gagcgctcgc tgtacgactg gcatagcggc gcgccggtgc cgtttaccga ccaggctttc
     1861 gaatcgatga cgcggcggtc cggataattc gcgtgtgtgg gttatggccc acaatatttt
     1921 gcggcgatat tattgggcag aatccttgag atttcgatag tgacggttta ggctgacgac
     1981 tcgaccagca gccggagtcg gttcgatgac ccgcaccacc agcaaactcc tcgatgaagt
     2041 ggaagccttt tgccgccgcg cagggatcgc ggagacgact ttcggccgcg aagcggtcaa
     2101 cgacggcaag ctgtgcgcgc ggctgcgcgc cggcaagagc ctgaccctgg aaaccgccga
     2161 ccgtattcgc gactatatcg ccagccacgg cggtgacggc aaggctacca cggatgacga
     2221 tgcgcccgtc gagccgctcg gcggacggca gcaactcgag acgcgccacc tcactgacga
     2281 ccggccattc cgcttctatg acaaccgcca gaagtacctg gccttcgtca acacctgcaa
     2341 tgagaaatgg aaggtcggcg aacgcgccac gcaggaattg tcgcatatac acccggtgcc
     2401 gccggcgctg cggctgttcg atgccggggt cggcgatggc accgtgctcg gccacctgat
     2461 gcgcgcgatg caccgtcgct ttccgaccat tccgtttttc atcgtcggca aggaaatcag
     2521 cctcgaagat gtccgcctga cgctggaaaa gttgccggac cgcttcatcg aacacccgtc
     2581 gagcgtcatc atcattacca acctgtacta tgccgaggca ccctggctga tgcccaacga
     2641 tgtcgccaag gcggcggcgc tgaactggat cgacatgccg ctacagggcg attcggccta
     2701 cgagttcggc gaacagctgc gtggcatcga caaggaactg gtcgacggct ggcaggtcaa
     2761 ggcgagcgaa aagtccggca acccgatgta tgtgcggccg tcggtactgg tgatgtaccg
     2821 cgaagaccat cgcttcctgc tcgacgacgt gattccacgc cgcggccggg ccgccggcgg
     2881 cgattacgac ctggtgctcg cctcgcagcc gtggcgcgcg cgcatggacg ccgagttcaa
     2941 ggtcaggaac gtgctcgggc cgctggtcag gagcctgcgc gcgggcggcc gggtactgac
     3001 ggttcagtcc agcggcgacg atccgggcct cgaactgatt cgccgcatct ggcccgacga
     3061 ggacccgttt cgcgtcaacc gccacgaact gatcaaggtg ctcaaggagc accttggccg
     3121 cagcgcgcaa aattacaatt tcaacgtcgg ctccgacgcc aagtcgattt ttcgctaccg
     3181 catgcacacg ctgccgaccg agatcggcga tgcgatcggc acctcgacgc tgttcgccgc
     3241 ctggaacgca gcgatctacg tcggccagat cgaggacgaa cgcctcgagc cggtggtctc
     3301 ctcgggagca tatctcgacg ccaccgccga catcctgcac cagcacaacg gtatctggtt
     3361 caacgacgaa accttcgtcg tctcgcgcaa gcgcgacgcc tgaatcgcgg cgcgacgcga
     3421 attcaactgc tgacagtgat tgccggacaa gcgcgaatct gctgccgcgc ggttaggaga
     3481 tccccgcttg cgcggggatg actcgggcag gcatgcgggg atgactcggg caggcatgcg
     3541 gggatgactc gggcaggtat gcgggggatg actcg
//
LOCUS       DATCZV010000250         2843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_125487, whole genome shotgun sequence.
ACCESSION   DATCZV010000250 DATCZV010000000
VERSION     DATCZV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125487"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VIW27_05505"
     CDS             complement(<1..978)
                     /locus_tag="VIW27_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY5739159.1"
                     /translation="MSKYSIFSLLRNAVSYHENWAEAWRSPEPKPEYDVLIIGGGGHG
                     LATAYYLAKEHGVTNVAVLEKSYLGGGNTGRNTTIVRSNYLWDESAHLYEKSMKLWEG
                     LSQDLNYNVMFSQRGVYNLGHTLQDMRDIHRRVNANRLNGIDGRVVTPAEIKRAIPEI
                     NIDQGCRYPILGSSFQPRGGVARHDAVAWGFARGADRCGVDIIQNCEVTAIRQQNGAV
                     TGVETTRGAIRAKKIGVVVAGHCSVLAEMAGFRMPIESHPLQALVSEPIKPIIDTVIM
                     SNAVHGYISQSDKGDLVIGAGIDHYNGYGQRGSFPTIEHTIAAICELFPI"
     gene            1170..2117
                     /locus_tag="VIW27_05510"
     CDS             1170..2117
                     /locus_tag="VIW27_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739160.1"
                     /translation="MFKDNATASAMAMMIVAMLMLPGIDAIAKWLSGAVSSGQVTWSR
                     FFFQVIFMSPLLWLTRGPWWTPALPLHAARGALIALATLCFFTGLAYLPMADAIAIFF
                     IEPLLVTLLSALFFGEPIHWRRISAIALGFVGALIIIRPTFADVGYAALLPVGAATCF
                     SFYILLTRKLVANEDPIRLQFFAGIFGCLVMAAGLAWGTASDVPILTAAWPSYDQWLL
                     LAALGLIGATCHLLVVYAYRSASIGILAPFQYVEIIAATILGLVIFGDFPDSPTWLGI
                     AIIVGSGIYVFHREATLGRQQSKPAIAVAVQTGEQQAGR"
     gene            2223..>2843
                     /locus_tag="VIW27_05515"
     CDS             2223..>2843
                     /locus_tag="VIW27_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5739161.1"
                     /translation="MEIETKAVHAGYSPDPTTKAVAVPIYQTTAYAFDNAQHGADLFN
                     LAVLGNIYSRIMNPTNDVLEQRIAAMEGGVAALALSSGQAATTYAIQTIAEAGDNFIS
                     MSTLYGGTYNLFAHTFPQMGIEARFADPEKLDDMAALIDDRTKAVYCESLGNPAGNIV
                     DIEKICEIAHAHGVAVIVDNTVPSPYLCRPIEHGADLFNLAVLGNIY"
BASE COUNT          505 a          909 c          852 g          577 t
ORIGIN      
        1 gatcggaaac agttcgcaga tcgcggcgat ggtgtgctcg atggtcggaa agctgccgcg
       61 ctggccgtag ccgttgtagt gatcgatgcc ggcgccgatg accaggtcgc ccttgtccga
      121 ctggctgata tagccgtgca ccgcgttcga catgatcacc gtatcgatga tcggcttgat
      181 cggttcggaa accagcgcct gcagcgggtg gctttcgatc ggcatgcgga aacccgccat
      241 ctcggcgagc accgagcaat gcccggcaac caccacgccg atctttttcg cacgaatcgc
      301 gccgcgcgtg gtctcgaccc cggtgaccgc gccgttttgc tggcggatcg cggtgacttc
      361 gcagttctga atgatatcga cgccgcagcg gtcggcgcca cgcgcgaaac cccaggccac
      421 cgcatcgtgc cgcgcaaccc cgccgcgcgg ctgaaaagac gagccgagaa tgggatatcg
      481 acagccctgg tcgatgttga tctcgggaat cgcacgcttg atctccgcgg gcgtgaccac
      541 gcgcccgtcg atgccgttga ggcggttggc attgacgcgc cgatggatat cgcgcatgtc
      601 ctgcagcgtg tggccgaggt tatagacacc gcgctggctg aacataacgt tgtagttcag
      661 gtcctggctg aggccttccc acagcttcat cgatttctcg tagaggtgcg cggattcgtc
      721 ccacaggtaa ttggagcgca cgatggtagt attacggccg gtgttgccgc cacccaggta
      781 gcttttttcg agcaccgcga cattggtcac gccgtgctct ttcgcgaggt aataggcagt
      841 cgccagaccg tgcccgccgc cgccgatgat caacacgtcg tattccggct tcggctcggg
      901 actgcgccac gcctccgccc agttttcgtg gtaactgacg gcgttgcgca acagcgaaaa
      961 aatcgaatat ttgctcattt cgggaccgaa ttctgcaggc cggtagcata acaagcctgt
     1021 cacggcgggc aaagtcgaaa ccgtggccag cggggccagg cgtcggaaca agctaaatcg
     1081 atgccgcatt tattatagcc gcgcatggtt cccgacggcc gtgaattcaa ctagactttc
     1141 agccctgcca aacgggccgc tcgctttcca tgttcaaaga caatgccacc gccagcgcga
     1201 tggcgatgat gatcgtcgcc atgctgatgc tgcccggtat cgacgcgatc gccaaatggc
     1261 tgtccggggc ggtctcgtcg ggccaggtca cctggagccg gttcttcttc caggtcattt
     1321 tcatgtcgcc gttgctgtgg ctgacccgcg gtccctggtg gaccccggca ttgccgctgc
     1381 acgcggcgcg cggcgcgttg atcgcgctcg ccaccctgtg ctttttcacc ggcctcgcct
     1441 acctgccgat ggccgacgcg atcgcgatct ttttcatcga accgctgctg gtgacgctgt
     1501 tgtcggcgct gttcttcggc gagccgattc actggcgccg catctccgcg atcgcgctcg
     1561 gtttcgtcgg cgcgctgatc atcattcggc ccaccttcgc cgatgtcggc tacgcggcac
     1621 tgttaccggt cggtgccgca acctgctttt cgttttatat cctgctgact cgcaagctgg
     1681 tcgccaacga ggaccccata cggctgcagt ttttcgccgg catcttcggc tgcctggtga
     1741 tggctgccgg gctcgcctgg ggcacagcca gcgatgtccc gatcctgacc gccgcgtggc
     1801 cgagctacga tcaatggctg ctgctggcgg cgctcgggct gatcggcgcg acttgccacc
     1861 tgctggtcgt ctatgcctac cgctcggcgt cgatcggtat cctggcgccg tttcagtacg
     1921 tcgagatcat cgccgcgacc atcctcgggc tggtcatctt cggcgatttt ccggattcgc
     1981 cgacctggct cggcatcgcc atcatcgtcg gctccgggat ttacgtcttt catcgcgaag
     2041 cgacgctcgg ccggcaacag tcgaaaccgg caattgcggt cgccgtgcaa accggggagc
     2101 agcaagcggg ccgctgatat cggcggccgg tcaacggcca tagcaattcg gttgcagacc
     2161 ccggggacat cggtttaaat ggcccggatc atccctggat atcacacaac tggagaataa
     2221 caatggaaat cgaaaccaaa gcggtccacg cgggctacag cccggatccg accaccaaag
     2281 cggtcgcagt gccgatttac cagaccacgg cctacgcctt cgacaacgcc cagcatggcg
     2341 ccgatctgtt caacctggcg gtgctcggca atatttatag ccgcatcatg aacccgacca
     2401 atgatgtgct cgaacagcgc atcgcggcga tggagggcgg cgtcgcggcg ctggcgctgt
     2461 catcgggcca ggcggcgacg acttacgcca tccagaccat cgccgaggcc ggcgacaatt
     2521 tcatctccat gtcgacgctc tacggcggca cctacaacct gttcgcgcat acctttccgc
     2581 agatgggcat cgaggcgcgt tttgccgatc cggaaaaact cgacgacatg gcggcactga
     2641 tcgacgaccg gaccaaggcg gtttactgcg aatcgctcgg caacccggcc ggtaatatcg
     2701 tcgacatcga gaaaatctgc gaaatcgcgc acgcgcatgg cgtcgcggtg atcgtcgaca
     2761 acacggtgcc gtcgccctac ctgtgccggc cgatcgagca tggcgccgat ctgttcaacc
     2821 tggcggtgct cggcaatatt tat
//
LOCUS       DATCZV010000251         7769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_20239, whole genome shotgun sequence.
ACCESSION   DATCZV010000251 DATCZV010000000
VERSION     DATCZV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20239"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VIW27_05520"
     CDS             complement(<1..949)
                     /locus_tag="VIW27_05520"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY5739162.1"
                     /translation="MKVEFDIASDQIDGARDYQEDAYLVNNLGENDNGDICSLIIMAD
                     GMGGHAAGNVASNMVVATFNKTFQSQFPTTEVAGVLTEALNRSNDQIRASVKETPQLR
                     GMGCTMVTAYVENEDLYWVSVGDSHLYLIRDRELIKQNADHSYGAYLDMMKEQGMEVD
                     EQVGMSRNMLMSAMTGDEISSIDVTKIPVKLKPGDRVIVASDGLDTLGDGAIIQYSSW
                     SSTAKECVLALLKAVEEANKPNQDNTTLIVIDVKQRESKVGAAPLQPNQGTVGDFMLE
                     DTPDPLSFKWLVWLVVLALLGGGIYFGWQRGLLDDALQKG"
     gene            complement(1057..1470)
                     /locus_tag="VIW27_05525"
     CDS             complement(1057..1470)
                     /locus_tag="VIW27_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5739163.1"
                     /translation="MALVLTHRQKVLFGKRIVDSTAYQWQLPGGWIAAGETPRAAALR
                     ELREETGLDAQRLRFVGLTSNRFAADRQSISLYFEARCVDAERLYRAEPGNCLDWQWL
                     DWDEVVENLYLPLRLLKQTGYRPFSGPASGTYISI"
     gene            1589..2554
                     /gene="arsS"
                     /locus_tag="VIW27_05530"
     CDS             1589..2554
                     /gene="arsS"
                     /locus_tag="VIW27_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468699.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY5739164.1"
                     /translation="MRDTWPLLKTTDFPPLTRKSLKILQVNLGYLCNQSCLHCHVAAG
                     PTRKELMLRDNIEHIVEVLRQPSVNTLDLTGGAPEMNPMFRELVSRARELGITVIDRC
                     NLTILLEAGYEDTAEFLADNQVQIVASLPCYMEENVDGQRGKGVYDKSMRALELLNEL
                     GYGRPGSGLSLTLVYNPTGPYLPPPQESLEQDYKGFLAEHFGIRFNQLFTITNMPIAR
                     FGSTLLSKGEFENYMNLLKDSFSAANLGGLMCLNQLSIDWQGYVYDCDFNQMLNMNIR
                     HPQKRHKLHISEVLKTDLENIPVSIADHCYGCTAGQGSSCGGALA"
     gene            2547..3236
                     /locus_tag="VIW27_05535"
     CDS             2547..3236
                     /locus_tag="VIW27_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY5739165.1"
                     /translation="MPDRPRLSIVIPVLNEADCLARTLESIYADPWLREHAEVLVSDG
                     GSEDASLEIAARYPCRIVGGAAGRALQMNAASDKARGDRLLFLHADSHLPADLGTLLN
                     DAAEWGFFRLRLSGDNGVFRVIETAINLRTRLTGIAGGDQGLFFERRLFDKLGGFANI
                     PLMEDIAICKLARRHAAPHIVSARITSSSRRWQQHGVIRTVLLMWWLRFAYWLGADPR
                     RLHRIYYPQRG"
     gene            3375..3974
                     /locus_tag="VIW27_05540"
     CDS             3375..3974
                     /locus_tag="VIW27_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY5739166.1"
                     /translation="MIPVGVFAKPPLPGLVKTRLMADIGAAKATRVYRYCLGYALDVA
                     RHSGLDYAVYLSQESDEAPFNEEQYRLQKGRDLGERMLNALQDMLDDGASAAIIIGSD
                     CLDLGAQRLQQAAQALASHELVLIPAVDGGFALIGCREADPELFRSVHWGTSRVLEQT
                     LANAHRLRYRVSLLESVRDIDTLQDLEHYPELLALIASS"
     gene            3974..4969
                     /locus_tag="VIW27_05545"
     CDS             3974..4969
                     /locus_tag="VIW27_05545"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY5739167.1"
                     /translation="MTYSLIKPLLFAVDPELAHDVSLEMLQQFHRLLPQRRIQQPTRV
                     MGLDFPNPVGLAAGLDKNADYVDGLARLGFGFIEVGSVTPRPQPGNPQPRVFRLVRQQ
                     SLINRMGFNNKGVDYLVERVRDKPRDFVLGINIGKNRDTPLDDALSDYCLGLEKVYPY
                     ADYVAINISSPNTPGLRELQNETALDNLLAAIGEQRRRLEDRHQLRRPLALKLSPDLD
                     QRAVPAVAEIMRRHAVDGLIATNTTLDREPVSGHRLAAEAGGLSGAALRERSREVLSA
                     FSRELSGEIPIISVGGIDSVDEARLRFELGASLIQLYTGLIYRGPGLVNRIVGSL"
     gene            complement(5026..5244)
                     /locus_tag="VIW27_05550"
     CDS             complement(5026..5244)
                     /locus_tag="VIW27_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739168.1"
                     /translation="MTNCPVHDGQRQLLDLQVFVRQIQPRGYFQQFAEQQKTEVADLG
                     SLVAVDQRSSAASSSRCSSWRGRSSRYS"
     gene            5300..5662
                     /locus_tag="VIW27_05555"
     CDS             5300..5662
                     /locus_tag="VIW27_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739169.1"
                     /translation="MRDRDALLAVSELLADIVEHCAPELKQGREGDDDSSSWRDLHAC
                     RGEVAIRSGDPGAIRRAFDEFLLYLPQQAREFFVACMKKAESGDYPPLARRMVADYLG
                     RTSAVLPWSAVLHSIDTR"
     gene            complement(5596..6423)
                     /locus_tag="VIW27_05560"
     CDS             complement(5596..6423)
                     /locus_tag="VIW27_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HEY5739170.1"
                     /translation="MNTEAGSPVSYSLELGGDRLRLNDWIGKDVRIEYLQEIECIHCG
                     RPTRKSFNQGYCYPCFANLAQCDSCIMSPEKCHFHLGTCREPEWGQAHCMRPHIVYLA
                     NTSGLKVGITRESQLPTRWIDQGAVQALPVLRVSKRYHAGLIEAAFRQHVNDRTNWRR
                     MLKNEVEQTDLYAVFESLWPTISENLEAELLDDAELVAAAGASVDFDYPALRYPQKVN
                     SFNLDKNPLAEGRLDAIKGQYLLLDSGVINIRKYGGYLVSMECKTALQGRTAEVRPR"
     gene            complement(6454..7110)
                     /locus_tag="VIW27_05565"
     CDS             complement(6454..7110)
                     /locus_tag="VIW27_05565"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF009732.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY5739171.1"
                     /translation="MVDKQHWLDRWRENRIGFHEAGVNRHLQAWLPHFGLEAGSCVFM
                     PLCGKAHDIAWLAAQGFEVIGIELSELAIEAFFEEQGLAFERFDSDRFGVYEAERITL
                     LQGDFFDLGNDDLGACSLVYDRAALIAMSASDRPHYYRHILSITPAVAGMLLITMDYE
                     QSEMQGPPFAVPEAEIRRYYEDTFSITLLHSEDIIDERPRWRQAGLSKLVEAVFRLDR
                     "
     gene            complement(7149..>7769)
                     /locus_tag="VIW27_05570"
     CDS             complement(7149..>7769)
                     /locus_tag="VIW27_05570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5739172.1"
                     /translation="DTMTTRNSATAADMKDLEMTDHSISVADQTLAPKDVVRRAKKDS
                     SKRTITLLSRENADKVAIDRYRTAAFEGEPLSILIIEVDGLSKLREQDGDQVAYKVFA
                     AIVKYIFSKVDKEKDILGRYGQNGLSVLMPRVDMNAAEKFAEMIRQFVASARYKTAYG
                     EKTATLSIGVAAITDDTGDYGSMVKRADMALFVAKKNGRNCVKVRL"
BASE COUNT         1506 a         2436 c         2298 g         1529 t
ORIGIN      
        1 tgcccttttg caacgcatcg tcgagcagtc cgcgttgcca gccgaagtaa atgccgcccc
       61 ccagcagggc gaggacgacc agccagacca gccacttgaa ggagagcgga tcgggagtat
      121 cttcgagcat gaagtcgccc accgtgccct ggttcggctg cagcggggcc gcgcccactt
      181 tcgactcgcg ttgcttgacg tcgattacga tcagcgtggt gttgtcctgg tttggcttgt
      241 tggcttcctc gacggctttc aacagcgcga ggacgcactc cttggcggtc gacgaccagg
      301 acgagtactg gatgatcgca ccgtcgccga gggtatcgag accatcgctg gcaacgatga
      361 ctcgatcgcc gggcttcagc ttgaccggga ttttggtgac atcgatactg ctgatttcgt
      421 cgccggtcat cgcactcatc agcatgttgc gcgacatgcc cacctgctcg tcgacttcca
      481 tgccctgctc tttcatcatg tcgagatagg cgccatagct atgatccgcg ttctgcttga
      541 tcagctcgcg gtcgcgaatc aggtagaggt gactatcacc gacgctgacc cagtagaggt
      601 cttcgttctc gacataggcg gtaaccatgg tgcaacccat gccgcgcagc tggggagttt
      661 ccttgaccga ggctcgaatc tgatcgttgg agcgattgag cgcctcggtc agtaccccgg
      721 cgacctcggt ggtcggaaac tggctctgaa aagtcttgtt gaaggtcgcc accaccatgt
      781 tgctggcgac attgccggcg gcatgcccgc ccatgccatc ggccatgatg atcagcgagc
      841 agatatcgcc attgtcgttt tcaccgagat tattgaccag gtacgcgtct tcctgataat
      901 cacgcgcacc atcgatctga tcgctggcaa tatcgaattc tactttcatt cggtgagttt
      961 cggtttttgg aatcgtgctc ctacggaact atagcagtat agttaaattt aattctagtt
     1021 tacggcttat aagcccataa aaaggctgaa aaaactctaa atcgagatat aggttccaga
     1081 ggcaggaccg gaaaacgggc gatagccggt ttgcttgagc aggcgcaacg gcaggtaaag
     1141 gttttcgacg acctcgtccc aatccagcca ctgccagtcg agacagttgc ctggctcggc
     1201 gcgatacagg cgttcggcat cgacgcatcg cgcctcgaaa tacagcgaga tcgactggcg
     1261 atcggccgcg aaccgattgc tggtcaggcc gacgaagcgc aagcgctgtg catcgagacc
     1321 ggtttcctcg cgcaactcac gcagcgccgc ggcccgcggc gtttcgccgg cagcaatcca
     1381 gccgcccggc agctgccact ggtacgcggt tgaatcgacg atgcgtttgc cgaacaatac
     1441 tttctgccga tgcgtcagga ccagcgccac gccgcaggcg acccggttct cgctgagctt
     1501 gatctgaacc tccccggtcc gacgttcgaa tgttgaactc tatgttatat tgcgagtcta
     1561 tgctggatca aatcacgagc accgatacat gcgcgacacc tggccgctgc tgaaaaccac
     1621 cgatttcccc ccgctgaccc gtaaatccct gaaaatcctg caggtgaatc tcggttacct
     1681 gtgcaaccag tcctgcctgc attgccatgt ggccgccgga ccgacgcgca aggaactgat
     1741 gctgcgggac aacatcgagc acatcgtcga agtgttgcgg cagccatcgg taaacacgct
     1801 cgacctgacc ggtggcgcac cggaaatgaa tccgatgttt cgcgagctgg tcagtcgcgc
     1861 gcgcgaactc ggcattaccg tcatcgaccg ctgcaacctg acgatcctgc tcgaagcggg
     1921 ctacgaagac accgccgaat tcctagccga caaccaggta cagatcgtcg cctcactgcc
     1981 ctgttacatg gaagaaaacg tcgacggcca gcgcggcaag ggagtctacg ataaaagcat
     2041 gcgagccctc gaattgctca acgagctcgg ctatggtcgg cccggcagcg gcctgtcgct
     2101 gacgctggtg tacaacccga ccgggcccta cctgccgcca ccgcaggaaa gcctggaaca
     2161 ggattacaag ggcttcctcg ccgagcattt cggcattcgc ttcaaccagc tgttcacgat
     2221 aaccaacatg ccgatcgcgc gctttggcag tacgctgctg tcaaaaggcg agttcgagaa
     2281 ttacatgaac ctgctcaagg attcgttcag cgccgccaac ctcggcggac tgatgtgcct
     2341 gaaccagctc agcatcgact ggcaggggta tgtttacgac tgcgatttca atcaaatgtt
     2401 gaacatgaac attcgccatc cgcaaaagcg ccacaagctg cacatcagcg aggtgctgaa
     2461 aaccgatctc gaaaacattc cggtcagcat cgccgatcac tgttacggtt gcaccgccgg
     2521 tcagggcagc agttgtggcg gggcacttgc ctgatcgccc gcgactcagc atcgtcattc
     2581 cggttctgaa cgaggcggat tgcctggcgc ggaccctcga gagtatttac gcggacccgt
     2641 ggctgcgcga gcatgccgaa gtgctggtca gcgacggtgg cagcgaggac gccagcctcg
     2701 aaatcgccgc gcgctacccg tgtcgaatag tgggcggtgc cgccgggcgc gccctgcaga
     2761 tgaatgccgc gagcgacaag gcgcgaggcg atcgcctgct gtttttgcat gccgacagcc
     2821 atctgcccgc ggacctcggc acgctcctca acgatgccgc cgaatgggga ttttttcgcc
     2881 tgcggctgtc gggtgacaac ggtgttttca gggtcatcga gaccgcgatc aacctgcgca
     2941 cccgactgac aggcatcgcc ggtggcgatc agggcctgtt tttcgagcgg cgcctgttcg
     3001 acaaactcgg cggctttgcg aacattccgc tgatggaaga tatcgccatt tgcaagctgg
     3061 cgcggcggca cgcagcgccg cacatcgtca gcgcgcggat taccagctcc agccgccgct
     3121 ggcagcagca cggcgtcatc aggaccgtgc tgttgatgtg gtggctacgc ttcgcctact
     3181 ggctgggcgc cgacccgcgc cggctgcacc gaatctacta cccacagcgt ggctgagtgc
     3241 agcgccgcag acgagcctgg cccagcccgt gaatacacgg gcctgcaggc ccgggccact
     3301 tactgcgccg cgccgccggt gacaggcgcc ggcatggttc gaacccggtc ggcgcgcggc
     3361 gcgcggtttc gccgatgata ccggtcggcg tatttgccaa gccgccgctg ccggggctgg
     3421 taaaaacacg actgatggcc gatatcggcg ccgccaaggc cacacgggta tatcgctact
     3481 gcctcggcta cgcgctcgac gttgcgcgcc attcgggact cgactatgcg gtctacctga
     3541 gccaggagtc cgacgaggct ccgttcaacg aagagcaata ccgcctgcag aaaggcaggg
     3601 atctcggcga gcgcatgctc aatgcactac aggacatgct cgacgacggc gcttcggcgg
     3661 caatcatcat cggcagcgac tgcctcgacc tgggagcgca gcgattacag caggcggcgc
     3721 aggcactggc gagccacgag ctggtgctga ttcccgccgt cgacggcggc ttcgcgctga
     3781 tcggctgccg cgaggccgac ccggagttgt tccgctccgt tcactggggt acgagccgcg
     3841 tgctcgaaca gacgctcgcc aatgcgcacc gcctgcgtta ccgcgtcagc ctgctggaaa
     3901 gcgtgcgcga tatcgacacg ctgcaggatc tcgagcatta tcccgagttg ctcgcactga
     3961 tcgcttccag ctgatgacct actcgctaat caaaccgctg ctgttcgccg tcgacccgga
     4021 actggctcac gacgtcagcc tggagatgct gcagcaattt catcgcctgc tgccgcaaag
     4081 acgcatacag caacccaccc gggtcatggg cctcgatttt cccaacccgg tcggactggc
     4141 cgccggtctc gacaagaacg ccgactatgt cgatggcctg gcgaggctcg gcttcggttt
     4201 catcgaggtc ggcagtgtaa cgccgcggcc gcagcccggt aatccgcagc cgcgtgtatt
     4261 ccgccttgtc cgccagcaaa gcctgatcaa ccgcatggga ttcaacaaca agggggtcga
     4321 ttacctggtc gagcgagtgc gcgacaagcc gcgcgatttc gtcctcggca tcaatatcgg
     4381 caagaaccgc gacacaccgc tcgacgatgc gctgtcggat tattgcctgg gcctggagaa
     4441 agtctatccc tatgccgatt acgtggcgat caatatctct tcgccgaaca cgccgggact
     4501 gcgcgagctg caaaacgaga cggcgctgga taacctgctg gcggcgatcg gcgaacagcg
     4561 ccgacggctc gaagaccgcc atcagctgcg tcgtccgctg gcattgaaac tgtcgcccga
     4621 cctggaccag cgcgcggttc cggccgtggc cgaaatcatg cgccgccatg ccgtcgacgg
     4681 cctgatcgcc accaatacga cgctcgaccg cgagccggta agcggccacc ggctggcggc
     4741 cgaggccggc ggcctgagcg gtgccgcgct gcgcgaacgc tcacgcgagg tactgtctgc
     4801 gttttcccgc gaactgagcg gtgaaattcc gatcatctcg gtcggcggca tcgattcagt
     4861 cgacgaagcc cgcctgcgct tcgaacttgg cgccagcctg atccagttat acaccggcct
     4921 catttatcgc ggcccgggac tcgtcaatcg catcgtcggg tcgctgtaac cgcaagcagg
     4981 cgttttcagg agatcgacgg acgaggcaca atcgctgcgg caggctcatg aatacctgct
     5041 ggatctgcct cgccagctcg agcagcggct cgacgacgcg gcgctgcttc gctggtcgac
     5101 cgcgacgagg ctaccgaggt ctgcaacttc ggttttctgc tgctcggcaa actgctggaa
     5161 atagccacgc ggctggatct gcctgacgaa cacctggaga tcgagcagct gtcgctggcc
     5221 atcgtgcact gggcaattcg tcacggcgcg accctcaacg atccgcaacc cgtggtcaat
     5281 gcgatcgccc gccgcgccga tgcgcgatcg cgatgcgttg ctggcggtgt cggaattgct
     5341 cgccgatatc gtcgaacact gcgcgccgga attgaagcag ggccgcgagg gcgacgacga
     5401 ttccagctcc tggcgcgacc tgcacgcctg tcgcggcgaa gtcgcgattc gcagcggcga
     5461 tcccggcgcc atccgccgcg ccttcgacga attcctgctc tacctgccgc agcaagcccg
     5521 ggaattcttc gttgcctgca tgaaaaaagc ggagagcggc gattacccgc cgctggctcg
     5581 ccgaatggtc gcggattatc tcggccgcac ctccgccgtc ctgccctgga gcgccgtttt
     5641 acactccatc gacaccaggt agccgccgta cttgcgtatg ttgatgacgc cgctatcgag
     5701 caacaggtac tgccccttga tggcgtcgag ccgaccctcg gcaagtgggt tcttgtcgag
     5761 gttgaagctg ttgacctttt gcggataacg caacgcgggg tagtcgaagt cgacactggc
     5821 gccggcagcg gcgaccagtt ccgcatcatc cagcaactcc gcttccagat tttcgctgat
     5881 cgtcggccac aaggattcga agaccgcgta gaggtccgtt tgctcgacct cgttcttcag
     5941 catgcggcgc cagttggtgc ggtcattgac atgctggcga aaggccgcct cgatcaagcc
     6001 cgcgtgatag cgtttcgaga cgcgcaatac cggcagcgcc tgcaccgcac cctggtcgat
     6061 ccagcgcgtc ggtaactgcg attcgcgggt aataccgacc ttgagcccgg aggtgttggc
     6121 caggtaaacg atatgcggcc gcatgcagtg tgcctgcccc cattccggtt cgcggcaggt
     6181 gccgagatga aaatggcatt tctccggaga catgatgcag ctatcgcact gcgccagatt
     6241 ggcgaagcac gggtagcagt aaccctggtt gaaactcttg cgggtcggcc ggccgcaatg
     6301 aatgcattcg atttcctgca ggtactcgat cctgacgtcc ttgccgatcc agtcgttgag
     6361 tcgcagacga tcgccgccga gttcgaggct atagcttacc gggctgccgg cctcggtatt
     6421 cattttgtgc aagttaccct ggaacttcac ccatcacctg tcgagtcgaa acaccgcttc
     6481 gacgagtttg ctcaagcctg cttgccgcca gcgcgggcgc tcgtcgatta tgtcttcgga
     6541 gtgcaacagg gtaatcgaaa atgtgtcttc gtaatagcgg cgaatctcgg cctcgggtac
     6601 cgcgaacggc gggccctgca tctcggactg ctcgtagtcc atggtgatca gcaacatgcc
     6661 ggcgacagcg ggagtaatcg acagtatgtg ccggtaatag tgcggccggt cagacgcgga
     6721 catcgcgatc agcgccgcgc ggtcgtaaac caagctgcag gcacccagat cgtcattgcc
     6781 caggtcgaaa aagtctccct gcagcagagt aatgcgctcc gcctcgtaaa cgccgaaacg
     6841 atcggaatcg aaacgttcga aagcgagccc ctgctcttcg aagaacgcct cgatggcgag
     6901 ctcggacaat tcgatgccga tgacctcgaa accctgcgcg gccagccagg cgatatcgtg
     6961 cgccttgccg cacaaaggca tgaacacgca actgcccgct tcgaggccga aatgtggcag
     7021 ccaggcctgc aggtgacggt tgacccccgc ctcgtgaaag ccgatacgat tctcgcgcca
     7081 gcggtccagc cagtgctgct tatcgaccat gcgcgtcgct cctcagtatc gcggcgcgag
     7141 cgggttggtc acagacgcac cttgacgcag ttgcggccgt ttttcttggc cacgaacaac
     7201 gccatgtcgg cacgcttgac catcgacccg taatcgccgg tgtcgtcggt aatcgccgct
     7261 acgccgatac tcagcgtagc agtcttctcg ccgtaggcgg tcttgtatcg cgcactggcg
     7321 acgaactggc ggatcatctc ggcgaatttt tcggccgcgt tcatgtcgac ccgcggcatc
     7381 agcacgctga gaccgttttg cccgtagcgg ccgaggatat ccttttcctt gtcgactttg
     7441 gaaaaaatat acttgacgat cgccgcgaat accttgtacg caacctggtc gccatcctgt
     7501 tcacgcagct tggaaagacc atcgacctcg ataatcagga tcgacagcgg ctcgccctcg
     7561 aacgcggcgg tgcgatagcg gtcaatcgct accttgtcgg cgttttcgcg gctgagcagg
     7621 gtaatcgtgc gtttggagct atctttcttg gcccgtctga ccacgtcttt gggcgccaga
     7681 gtctgatcgg cgaccgagat gctgtgatcg gtcatctcga ggtctttcat gtccgccgcg
     7741 gtcgccgaat ttctggtggt catggtatc
//
LOCUS       DATCZV010000252         2795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_179495, whole genome shotgun sequence.
ACCESSION   DATCZV010000252 DATCZV010000000
VERSION     DATCZV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179495"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VIW27_05575"
     CDS             <1..1092
                     /locus_tag="VIW27_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5739173.1"
                     /translation="RQRLDGLDIDDALRTTLDLEAQTLHRLLGYRGQSFVHGPTNPLA
                     FDCVIVDEASMIDLKLMYHLLLALPPTARLILLGDRDQLASVAAGNVLGDITGHGHAI
                     DAGNTGVAGAVALLGGNYRFAGDSAIGRLAGHVNRGETDAAVELLAAAGQGLRWWQTP
                     GADSLDPAALEWICAAYLPIFTCRSAAAALDVYEASRVLCATNRGPLGVDAIGQRISA
                     ELLSRAGLPAATLYPGLPIMITRNHYSLGLFNGDSGILWRDAGGLRACFRSDDGVRAI
                     PLNRLPAYMPAWASTVHKSQGSEFDSVLLLLPADADSAALSRELLYTAVTRARRDFLL
                     QASPTVLSATVGRLTRRYSGLAARLGWPG"
     gene            1206..>2795
                     /locus_tag="VIW27_05580"
     CDS             1206..>2795
                     /locus_tag="VIW27_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HEY5739174.1"
                     /translation="MSSSSLGKALVVDDEKTNRLILKSLLSKQGYQTIEAVNGQQAVE
                     LFKQEGPTIIFMDIVMPVMDGYEATRQIKAASAGRFVPIIFLTAISDEQALAQCIEAG
                     GDDFLVKPYDKLILQSKIHSMQRIASLNHEVQGMYRMIKHEQEIAESVFINAIQSSNI
                     DNPHIRHIVRPAGIFSGDMVLSAYSPARDLFFMTGDFTGHGLLSALGAMPVSEVFSAM
                     TTKGFSAEEILVGINRKLKAMLPVGMFFGAQLVVIDHDLEYLRVYNAGMPDIIIVDGI
                     SNRIKHRLASNGLPLGVEAEIDPRQMVQYAPITHNDRILLYSDGLTEARNAGDQVYGS
                     KRLEAAISTAPTERIFESVIEDLDRFCGERSAQPDDITLAEIACVHDVLPKIKAHGAT
                     RPARRGDRGEWNLSLHLHGSRLRETNPVPLLVSYLLEMERLEAERKSLFTVLTELYLN
                     ALDHGVLGLDSSLKSDPSGFETYFRTREKRLTALDDGYVKFDLSVEQQAERRSILLRI
                     EDSGKGFDYEDRAQLTNWQMAL"
BASE COUNT          525 a          904 c          867 g          499 t
ORIGIN      
        1 cgccagcgcc tcgacgggct cgatatcgac gacgcgctgc gcaccaccct ggatctcgag
       61 gcgcaaacgc tgcaccggct gctcggctat cgcggtcaat cctttgtcca cgggccgacc
      121 aatccgctgg ctttcgattg cgtgatcgtc gacgaggcgt cgatgatcga tctcaagctc
      181 atgtaccacc tgctgctggc cttgccgccg acggcacgcc tgatcctgct cggcgatcgt
      241 gaccagctgg cgtcggtcgc ggccggcaac gtgctcggcg acatcaccgg ccacggccac
      301 gcgatcgatg ccggcaacac cggggtagcc ggcgcggtag ccctgctggg cggcaattat
      361 cgctttgccg gcgacagcgc catcggtcgc ctggcggggc acgtcaatcg cggcgaaacc
      421 gacgcggccg tcgagctgct ggccgccgcc gggcaagggc tgcgatggtg gcagacgccg
      481 ggcgcggata gcctcgaccc cgctgcgctg gaatggattt gcgcggcgta cctgccgatt
      541 ttcacctgcc gttcggcggc cgcggcgctc gacgtctacg aagccagccg ggtgttgtgc
      601 gcgaccaatc gtgggccact gggcgtggat gccatcggcc agcggatctc cgcggagcta
      661 ctgtcgcggg ccgggttgcc ggccgcaacg ctttacccgg gattaccgat catgattacc
      721 cgtaaccact attcgctcgg cctgttcaat ggcgatagcg gcatcctgtg gcgtgacgct
      781 ggtggtcttc gcgcctgctt tcgcagcgac gacggcgtac gcgcgattcc gctcaatcgg
      841 ctgcccgcgt atatgccggc ctgggccagc acggtgcaca agtcacaggg gtccgagttc
      901 gactcggtgc tgctgctgct gcctgccgac gcagactcgg cggcgctatc gcgggaattg
      961 ctgtatacgg cggtaacgcg cgcgcggcgg gacttcctgt tgcaggcctc gcccacagtg
     1021 ctgtcggcga cggtcggccg cctgacacgg cgttactcgg gcctcgctgc caggctcggc
     1081 tggcctggct gaagttcgag cgaccggctt gctttcgaag tcgtatatac taaacaggta
     1141 tgccaacctg caaggaggtc cccggggtcg cgaaccagtg ccggttttca cgaccgtaat
     1201 tcgccatgag tagttccagc ctcggcaagg ccctggtcgt cgacgacgaa aaaaccaacc
     1261 ggctgatcct caaatccctg ctcagcaagc aggggtacca gactatcgaa gcggtcaacg
     1321 gccagcaggc cgtcgagctg ttcaagcagg aaggcccgac cattatcttc atggatatcg
     1381 tgatgccggt gatggatggc tacgaggcga cgcgccagat caaggcagcc tcggcggggc
     1441 gtttcgtgcc gattattttc cttaccgcga tcagcgacga gcaagccctg gcacagtgca
     1501 tagaagccgg cggcgacgat ttcctggtca agccctacga caagctgatc ctgcagagca
     1561 agatccactc gatgcagcga atcgcgtcgc tgaatcacga ggtccagggc atgtaccgca
     1621 tgatcaagca cgagcaggaa atcgccgagt cggtgtttat caacgcgatc caaagttcga
     1681 acatcgataa cccgcacatt cgccatatcg tgcgcccggc cggcatcttc agcggcgaca
     1741 tggtattgtc ggcctattcg ccggcccgcg acctgttctt catgaccggc gatttcaccg
     1801 gccacgggct gctgtcggcg ctcggcgcga tgccggtatc ggaggtcttc agcgcgatga
     1861 ccaccaaggg cttcagtgcc gaggagattc tcgtcggcat caaccgcaag ctgaaggcga
     1921 tgctgccggt cggcatgttc ttcggcgcgc aactggtggt catcgaccac gacctcgagt
     1981 acctgcgcgt ctacaacgcc ggcatgcccg acatcatcat cgtcgacggc atcagcaatc
     2041 gcatcaaaca ccggttggct tccaacgggc tgccgctcgg tgtcgaggcg gagatcgacc
     2101 cgcggcagat ggtgcaatat gcgccgatca cgcacaacga cagaatcctg ttgtacagcg
     2161 acggcctgac cgaggcgcgc aacgccggcg accaggtgta cggcagcaag cgcctcgagg
     2221 cggccatctc caccgccccc acggagcgaa ttttcgaaag cgttatcgag gacctcgatc
     2281 gcttttgcgg tgaacgctcg gcgcagcccg acgacatcac gctggcggaa atcgcctgcg
     2341 tgcacgacgt gttgccgaaa atcaaggccc acggcgccac cagaccggcg cggcgcggcg
     2401 atcgcggcga atggaatctg tcgctgcatt tgcacggcag ccgccttcgc gaaaccaacc
     2461 cggtgccgct gctggtcagc tacctgctgg aaatggaacg gctcgaggca gagaggaaat
     2521 cgctgttcac ggtgctgacc gagctttacc tgaatgcact cgaccacggc gtactcggcc
     2581 tcgactcgtc actgaaatcc gacccatccg gattcgagac atactttcga acccgcgaga
     2641 agcggctcac agcgctcgac gacggttacg tcaaattcga cttatcggta gaacaacagg
     2701 ccgaacggcg cagcattctg ttacgcatcg aggacagcgg caagggcttc gattacgagg
     2761 accgcgccca gctcaccaac tggcagatgg cactc
//
LOCUS       DATCZV010000253         4736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_72595, whole genome shotgun sequence.
ACCESSION   DATCZV010000253 DATCZV010000000
VERSION     DATCZV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72595"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1264
                     /locus_tag="VIW27_05585"
     CDS             83..1264
                     /locus_tag="VIW27_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5739175.1"
                     /translation="MTIDKRAIRAELLSDTDYVHLNSAGASLMPRSVLQAQLDYLRLE
                     ARVGGYEAERRQQAELEAVYDSVARLLGCRPSEIALVENATVGWMMAFYAIPFAAGDR
                     ILTVEAEYASNYLAYLQIAREKGVVVDTIPSTADGEACVESLRGMIDERVRLISVTHV
                     PTNGGLVNPIEEIGAIAREHGILYLVDACQSAGQMPLIVERIGCDLLSATGRKFLRGP
                     RGSGFLYVSERILDRLHPPMIDLHSAEWVEVDRYQLRADARRFENWESNYAARLGLGR
                     AIDYALEIGLDKIAAEVVRLADRLRERLAEIPGVTVFDIGRRRCGIVTFAVNGVTADE
                     VESRLRDNRIHVSVTTPSGTLIDATRRQLPDMVRASVHYFNEDAEIELCANLVGKIAA
                     T"
     gene            complement(1292..1678)
                     /locus_tag="VIW27_05590"
     CDS             complement(1292..1678)
                     /locus_tag="VIW27_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY5739176.1"
                     /translation="MSYTIDRKITDADFDEVDSRARAALAAHGFGVLTEIDVKATMKK
                     KLDVDMAPYRILGACNPQMAHQAIGMEPRVGAMLPCNVILRAVDDGIEVSAIDPLASM
                     QAIDNEALKSIAGQVRDMLTQVVEEI"
     gene            complement(2012..4249)
                     /locus_tag="VIW27_05595"
     CDS             complement(2012..4249)
                     /locus_tag="VIW27_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY5739177.1"
                     /translation="MTASKLKVISVTDGSRPSHVNANNSASQGGAATLDAIGRLALTG
                     LDGLLHQFMDRVDDAFFERSDKARSEQERKRYFDSMREFRNQRDELHAYFAKQMKRSL
                     AELARHSTYAAGHDDDMTRIEIAGTDDRTAIDNLISRARLKFAGDLSTVSAGIKALLG
                     MTQLDPDDNPFDPGPICENFHAACALLQIDIAVKLIFYKLFEKHLLAGLGGFYLELNR
                     QFTEQGVLPAIGAGNARDKSASAAATPADRNSPLVDALTRLQKTADINHSPSSVDPAK
                     FRRSVRQQVDALCRQNRKWLSARDTKTVEAVSMLFDFFFEDEGLPDPIKVLIGRLQVP
                     ILKVAIIEPDFLTRKQHAARRLLDTVSRASLCWSDNQRDTRLLIGKTREVVDFIVARY
                     ARDAVVFDKACGDFESFVVRESEKRDHAEATLREREQHRDQQISLAREAAKTLIGRLA
                     AGRELDAEIVDFLHTTWTSVLVRTYLSLGDSSNHWRNLKRISTTLVWTLIPRHTEAER
                     DKIIRTIPALLRALSKGMALIRTEMHIQNRVFAVLAREHARVVKQTSRNIVTRIDDIT
                     IWPEDEEERALAMTGNIDEQEIAVTYVDPDRIDSDADAISLIDSIGTNEVIKNLNQFT
                     SGVSKGSIRIDEEIVLSGENDIEFEFEEGTRDKYLELASELPTGSWVEFVRPDESRQL
                     LKLAWKSAAAGTLVFVNSNGQKVKNMTVNDFAAELRAKSVRCARSSSTVDRAFFRIAT
                     RLDER"
     gene            4539..>4736
                     /locus_tag="VIW27_05600"
     CDS             4539..>4736
                     /locus_tag="VIW27_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739178.1"
                     /translation="MRFFTYISALLAVCAAANAAAAGPQRIVSIGLCTDQLLLLIAER
                     EQIASLSNWAVNADMSYLAGEV"
BASE COUNT          807 a         1386 c         1498 g         1045 t
ORIGIN      
        1 ccctatgaca cggtatgcgc attgccgccg ggacaggagt cggtatgaga cgcggcggcg
       61 aaccgcggag gagcgatcgg cgatgactat cgacaagcga gcaatacgcg cggagttgct
      121 gtccgatacc gattacgtcc acctcaacag tgccggtgcc tcactgatgc cgcgcagcgt
      181 gctgcaggcg caactcgatt acctgcggct cgaggcgcgg gtcggcggct atgaggccga
      241 gcgtcggcag caggcggagc tcgaagcggt ttacgattcg gttgcgcggt tgctcggttg
      301 ccggccgagt gaaatcgcgc tcgtcgaaaa cgccacggtc ggctggatga tggcgtttta
      361 cgcgatcccg ttcgcagccg gcgatcgcat tttgacggtc gaggccgagt acgcgtcgaa
      421 ttacctggcc tacctgcaga tcgcgcgcga aaagggagtc gtcgtcgata ccattccttc
      481 taccgccgac ggcgaggctt gcgtcgaatc gttgcgcggc atgatcgacg agcgcgtgcg
      541 gctgatctcg gtgacccatg ttccgaccaa cggcgggttg gtcaacccga tcgaagagat
      601 cggtgcgatc gctcgcgaac acggtattct ctacctcgtc gacgcctgcc agtcggccgg
      661 ccagatgccg ttgatcgttg agcgaatcgg ctgcgatctg ctcagcgcca ccggccgcaa
      721 gttcctgcgc ggcccgcgcg gcagcggttt cctctatgtc agcgagcgca ttctcgatcg
      781 gctgcatccg ccgatgatcg acctgcactc ggccgagtgg gtcgaggtcg atcgttatca
      841 gctgcgtgcc gatgcgcggc gcttcgagaa ctgggaaagc aattatgccg ccaggctcgg
      901 gctggggcgc gcgatcgact acgcgctcga gatcggcctc gacaagatcg ccgccgaagt
      961 ggtccgtctc gccgaccgtc tgcgcgaacg gctggcggaa attccgggtg tcacagtgtt
     1021 tgatatcggc cgccgcaggt gcggcatcgt gacctttgcg gtgaacggcg tgaccgcgga
     1081 cgaggtagag tcacggctca gggataaccg aatccatgtc agcgttacca cgccttccgg
     1141 tacgctgatc gatgctactc gccggcaact gcccgacatg gtgcgcgcct cggtgcacta
     1201 tttcaacgag gatgcggaaa tcgagctttg tgcgaacctg gtcggcaaga tcgccgcgac
     1261 ttgagattta cccgcggcga cttgcggcga atcagatttc ttcgacgacc tgggtcagca
     1321 tatcgcgcac ctgtccagct atcgacttca gcgcttcgtt gtcgatcgcc tgcatcgatg
     1381 ccagcgggtc gatcgcgctg acctcgatac cgtcgtcgac agcgcgcagg atcacgttgc
     1441 agggtaacat cgcgccgacc ctgggctcca tgccaatggc ctggtgggcc atctgcgggt
     1501 tgcaggcgcc gaggattcgg tagggggcca tatcgacgtc gagtttcttt ttcatcgttg
     1561 ccttgacgtc gatctcggtc agcacaccga atccgtgtgc ggcgagtgcc gcgcgggctc
     1621 ggctgtcgac ttcgtcgaaa tccgcgtcgg taatcttcct gtctatggtg taactcattc
     1681 cgctaatctc ccggactggc tgccgggccc ggtacgggcc cgcaggcaaa aaagggtaag
     1741 ggtaccaaat tggctgcaag attccagatc gcggaatcgc attgtcgcga tttgacgctc
     1801 ccgggttgtc ggtcaggctg agcgccgcgg accggaggga gctgctgtgt cggtcgaagt
     1861 gtttgttgct aggtcggcag cgaggcagtg atccatccga ttagcatcag taccagcatt
     1921 acgttgacca gttgtaaata tatcttcatt gtggtgtcgg ttgtgttcaa attccatgtt
     1981 aaacgtggtc cccccggtac ccgttttatc tttatcgctc gtctagtcta gttgcgattc
     2041 taaaaaatgc gcggtccacc gttgaggaac tgcgtgcaca gcggacgctc ttggcgcgca
     2101 gttcggcggc gaagtcgttg accgtcatgt tcttgacctt ctggccgttg ctgttgacga
     2161 ataccagtgt gccggccgcg gcgctcttcc atgccagttt caggagctgg cgcgattcgt
     2221 cggggcgaac gaactccacc cagctgccgg ttggcagttc gctggccagt tccaggtact
     2281 tgtccctggt gccttcctcg aattcgaatt caatgtcgtt ttcgccgctg agcacgattt
     2341 cctcgtcgat gcggatcgaa cccttgctga cgccgctggt aaactgattc agattcttga
     2401 tgacttcgtt ggtgccgatc gaatcgatca gcgagatggc gtcggcatcg gagtcgattc
     2461 gatccgggtc gacgtaggtg accgcgatct cctgctcgtc gatattgccg gtcatcgcca
     2521 gggctcgttc ctcttcgtcc tcgggccaga tggtgatatc gtcgatccgg gtgacgatgt
     2581 tgcgcgaggt ctgcttgacg accctggcgt gctctctggc cagcaccgcg aacacccggt
     2641 tctggatatg catttctgtc ctgatcagtg ccatgccctt tgataaggcg cgcaacaacg
     2701 ccggtatggt tctgatgatt ttgtcgcgtt cggcctcggt atgtcgcgga atcagcgtcc
     2761 acaccaacgt cgtcgagatg cgcttgagat tgcgccagtg gttggacgag tcaccgagcg
     2821 acaggtaagt cctgaccaga accgaggtcc aggtggtgtg caggaaatcg acgatttccg
     2881 catccagctc gcggcccgcg gccaacctgc cgatcagcgt cttggcggcc tcgcgcgcca
     2941 ggcttatctg ctggtcccta tgctgctcgc gctcgcgcag tgtcgcttcc gcgtggtcac
     3001 gtttttcgct ctcgcgaacc acgaaacttt cgaagtcgcc gcaggccttg tcgaatacca
     3061 cggcatccct ggcgtagcgg gcgacgatga aatcgacaac ctcgcgtgtc ttgccgatca
     3121 gcagtcgcgt atcgcgctgg ttgtcgctcc agcagagcga ggcccgcgaa acggtatcca
     3181 gcagcctgcg cgccgcatgc tgttttcgag tcaggaaatc gggctcgatg atggcgacct
     3241 tgagtatcgg cacctgcagc cggcctatca gcaccttgat cgggtcgggc agcccttcgt
     3301 cctcgaaaaa gaagtcgaac agcatcgaaa cggcttcgac ggtcttcgta tcccgggccg
     3361 acagccactt ccggttttgt cggcacagtg catcgacctg ctggcgaacc gaacgcctga
     3421 atttggccgg gtcgacgctg cttggcgagt gattgatgtc ggcggttttc tgcaggcggg
     3481 tcaaggcatc gacgagcggg ctgttgcggt ctgccggcgt cgccgcggcg gatgccgatt
     3541 tgtcgcgagc attgccggcg ccgatagccg gcaacacacc ctgctcggta aactggcggt
     3601 tcagttcaag gtaaaaaccg ccgaggcccg ccagcaggtg tttttcgaat aatttgtaga
     3661 agatcagctt gaccgcgata tcgatttgca gcagcgcgca ggcggcgtgg aagttttcgc
     3721 aaatcggccc gggatcgaac gggttgtcgt ccgggtcgag ctgggtcatg ccgagcaggg
     3781 ccttgatgcc tgcgctgacc gtgctcaggt cacccgcgaa cttgagccgc gccctcgaaa
     3841 tcaggttatc gatcgctgtc ctgtcgtcgg tgccggcgat ttcgatccgg gtcatgtcgt
     3901 cgtcgtgccc cgcggcatag gtgctgtgac gggcgagttc ggcaagcgag cgtttcatct
     3961 gcttggcgaa gtatgcgtgc agctcgtcgc gctggttgcg gaattcgcgc atcgaatcga
     4021 aatagcgttt gcgttcctgt tcgctgcggg ctttgtccga gcgctcgaag aaagcgtcgt
     4081 cgactcggtc catgaattgg tgcagcaggc cgtcgagccc ggtgagcgcg agccggccga
     4141 tcgcgtcgag ggtcgcggca ccaccctgtg aggcggaatt attcgcgttg acgtggcttg
     4201 gcctgctgcc gtccgtgacg cttatgacct tgagcttgga tgctgtcatc gaacttgctg
     4261 tcgcctctct ggcctgtccc ggcactcgat cgtctggagc cggcaggggt tatatttccg
     4321 ctgtctagcg gttgcgctcg ggtgtgatag ggatcacatt tttctctagc gtcagttgag
     4381 gatagtcgct tatcggcccg cggcacgacc gctggtagcc gaaatgccac cgtctgtcgg
     4441 gaaaaattga ccgaacaagg cgcaaaccgc gctgcgccgg ggtttactgc cgagcgccaa
     4501 tccggacaat agccgggatc tgtgacaagg ggtagacgat gcgttttttt acctacatct
     4561 cggcgctgct ggcggtttgc gcggccgcca atgccgccgc tgcgggcccg caacgcatcg
     4621 tctcgatcgg tctttgcacc gaccagttgc tgttgctgat cgccgagcgc gagcagatcg
     4681 catcgctgtc gaactgggcg gtgaatgccg acatgtccta cctggcgggc gaggtg
//
LOCUS       DATCZV010000254         6524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_179552, whole genome shotgun sequence.
ACCESSION   DATCZV010000254 DATCZV010000000
VERSION     DATCZV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179552"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VIW27_05605"
     CDS             complement(<1..916)
                     /locus_tag="VIW27_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5739179.1"
                     /translation="MTAALNDCVDYSPFVLKNEGEYRAWRARKLALRRQLPADRVFEL
                     DADGGVPRASIEALRLQIDAHNFAIFESPRELDKTAFLALNRQFGLCRLDCSSGADED
                     GVTCLRVVDDADRRAQYIPYTNRALSWHTDGYYNPPGQRINAFALYCVNQASRGGGNH
                     LFDHELMYILVRDRAPGLLAALMAEDMMRIPANIQDNRVVRAEETGPVFALQTGGCNL
                     LMRYTSRPRNIVWKADRRSAQALDLIREILLDGEAAIDIRLQPRQGMVCNNVLHGRQA
                     FHDSDFDPARLVYRARYHDSIDFDRQPVA"
     gene            1324..2583
                     /gene="dsrA"
                     /locus_tag="VIW27_05610"
     CDS             1324..2583
                     /gene="dsrA"
                     /locus_tag="VIW27_05610"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HEY5739180.1"
                     /translation="MAKKIHVTPMLDELENGPWPSFISGIKRLRDEHSDQRINGVANS
                     LLGQLEHSYETRKGYWKGGTVSVYGYGSGIIPRFSEVAQAFEESKEFHTLRVQPPAGN
                     YYTTDMLRQLGDSWEKYGSGLVTFHGQTGNIMFIGTSTENTQHFFDEINDYGWDLGGA
                     GPCVRTGMSCVGSGRCEQSCADEMRIHRTLLNNFTDDVHRPALPYKFKFKVSGCGNDC
                     MNSIERSDFALIGTWRDDMKVDQEAFKEYVAKKGRQHTIDNIITRCPTKALSLNDDDT
                     LEVDNRNCVRCMHCLNVVPKALSPGDDKGVSILIGGKRTLKIGDLMGTVIVPFMKLED
                     EEDFEALTELAEEVIDFWAENGLEHERCGEMVERIGLVNFLEGIGVEVDPNMISETRS
                     SSYVRMDDWDIEAKKWFDRKAEHKKAS"
     gene            2640..3710
                     /gene="dsrB"
                     /locus_tag="VIW27_05615"
     CDS             2640..3710
                     /gene="dsrB"
                     /locus_tag="VIW27_05615"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HEY5739181.1"
                     /translation="MEGKRTPIESGCPDGFQYMHPLMRKNYGNWAYHEDPRPGVLKHV
                     SWSGDAIHTVRAGTQRILDVFTLRKLCDIGDQYADGYVRFTIRSNIEYMVADESKVAP
                     LIEALEKEGFVVGGTKNSVAMISHTQGWLHCDIPGTDASGVVKSMMDELIDEFKNWNM
                     PNRVHITTSCCQINCGGQGDIAINIQHTKPPKINHDLVANICERPSVVARCPVAAIRP
                     ALVNGKPSLEVDEKKCICCGACYPPCTPMQINDPEHTKLAIWVGGNHSNARGKPTFQK
                     LVAAGIPNNPPRWPEATAVVKKILQTYKDNARDWERLNDWIERIGWPRFFELTELPFT
                     KYHVDNWRGARRTLNASTHIRF"
     gene            3724..4116
                     /gene="tusD"
                     /locus_tag="VIW27_05620"
     CDS             3724..4116
                     /gene="tusD"
                     /locus_tag="VIW27_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HEY5739182.1"
                     /translation="MKFGILVNEGPYTHQASDTAYHFTAAALKAGHEVVRVFFYHDGV
                     NNGTRLGVPPQDDRNITERWSELAKDYDVELILCVAAAQRRGLLDADEAKRAGKDVDN
                     MAPGFQISGLGQLIDAGIQADRLVVFGD"
     gene            4119..4523
                     /gene="tusC"
                     /locus_tag="VIW27_05625"
     CDS             4119..4523
                     /gene="tusC"
                     /locus_tag="VIW27_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HEY5739183.1"
                     /translation="MATKKFLYINRKPPHGSIYALESLEVVLIGAAFEQDVSLAFVDD
                     GVYQLLRNQDTGDIGTKNFSPTYRALGDYEVSKIYVERESLELRGLSKDDLMELTYED
                     EDDDWAEKDSIRIVDSEELGAIIEQQDVLLNF"
     gene            4534..4842
                     /gene="tusB"
                     /locus_tag="VIW27_05630"
     CDS             4534..4842
                     /gene="tusB"
                     /locus_tag="VIW27_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HEY5739184.1"
                     /translation="MPVLHIINKSPYERGSLDTCLRLAQDDAAILLIEDGVYALRNNA
                     ATAEKLRAALGKHKIYALEPDLKARGLAPESALEGVSLVDYDGFVELATEYDKLQSWL
                     "
     gene            4878..5210
                     /locus_tag="VIW27_05635"
     CDS             4878..5210
                     /locus_tag="VIW27_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEY5739185.1"
                     /translation="MAIELGGKTYETDEEGYLVNLSDWNDELAEEMAKADGAELGENH
                     WEVINFLREYYEEYQIAPAVRVLTKAVGKKLGKDKGTSKYLYELFPYGPAKQACKYAG
                     LPKPTGCV"
     gene            5542..6279
                     /locus_tag="VIW27_05640"
     CDS             5542..6279
                     /locus_tag="VIW27_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY5739186.1"
                     /translation="MSLLTLAYTLLFYAASLVLLAGLAFRIRLYARTPTPLRIPTTPA
                     PTTSAGVVRRMFFEVVLFNSLFKASKWTWLFGWLMHFALLLVLLRHLRYFTDPVWSWV
                     AFLQPFGFYAGFAMLAGLGGLWARRLLVDRVRYISAPSDHLMLALLAGIVLTGLGMSY
                     VAPTDIVAVKAFFLGLMYFDWQPMPADWMLNLHLLLVALLMLVFPFSKLLHAPGVFFS
                     PTRNQVDNPREHRHLPTRGPAAADSGN"
     gene            6297..>6524
                     /locus_tag="VIW27_05645"
     CDS             6297..>6524
                     /locus_tag="VIW27_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5739187.1"
                     /translation="MAEFETPALKPWPEIPPIEIGAMAHSKPFVAKQEHQQKIGFPGE
                     LVDNWQQVAIDKLGELVNNSRGLRVFLDSCVK"
BASE COUNT         1376 a         2009 c         1911 g         1228 t
ORIGIN      
        1 ccgcgaccgg ctggcggtcg aaatcgatgc tgtcgtggta gcgcgcccgg taaacgagcc
       61 gagccgggtc gaaatccgaa tcatgaaatg cctgccgccc gtgcagaaca ttgttgcaca
      121 ccatcccctg ccgcggctgc aagcgaatat cgatcgccgc ctcgccgtcg agcagaattt
      181 cgcgtatcag gtcgagtgcc tgcgcactgc gtcgatcggc cttccagaca atattgcgcg
      241 gcctcgacgt gtaacgcatc agcaggttgc agccgccggt ttgcagtgcg aataccggcc
      301 cggtttcctc ggcacgcacc acccggttgt cctgtatgtt ggccggaata cgcatcatgt
      361 cctccgccat caacgccgcc agcaaacccg gcgctcgatc gcgcaccagg atatacatca
      421 attcgtggtc gaacaggtga ttgccacccc cgcggctcgc ctgattaacg caatataacg
      481 caaacgcgtt gattcgctgc ccgggcggat tgtaatagcc gtcggtatgc cagctcagcg
      541 cgcggttggt gtagggaatg tactgggcgc gacgatcggc atcgtcgacg acccgcaaac
      601 aggtcacgcc atcctcgtcg gcaccgctgc tgcaatccag ccggcacagg ccgaactgcc
      661 tgttgagcgc caggaaggcc gtcttgtcga gttcgcgcgg cgactcgaaa atcgcgaaat
      721 tgtgcgcgtc gatctgcaat cgcaacgcct cgatcgacgc ccgcggaaca cctccatcgg
      781 catccagttc gaatacccgg tcggcgggca gctgccgcct cagcgcgagc ttgcgcgcgc
      841 gccaggcgcg gtattcaccc tcgtttttca acacgaacgg cgaatagtcg acgcaatcgt
      901 tgagtgcagc ggtcatttgg tctactgttg cgttcaagat cgttccgggc gccggttgac
      961 ggagtactaa cttcggtgat tctattacac gcccggcccg attggagcat cccatcggga
     1021 ggagttcgaa aagctattgg agaaatagtt caagtagctg ataactaaca attatttgca
     1081 atattgggta aatcgggtag aggtcgccga acaggctcgc aatcgagcct atctcgatgg
     1141 ggataagttt gcctatcaca gcaccccaac cgggggatgg ctcatccggc gcagttctac
     1201 caaactaccc ggtgttgccg gtcacatgcc gcagccgacc ctgtcggacc aacagagcga
     1261 attcggcatc ttcggcctgc tactccggga atttctcata ccagacatac gggaacgata
     1321 aacatggcta agaaaattca cgtcactccg atgcttgacg agctcgaaaa cggaccctgg
     1381 cccagtttca tttccggtat caagcgattg cgtgacgagc actccgacca gcgtatcaat
     1441 ggcgtcgcca attccctgct cggtcagctc gagcactcct acgaaacccg caagggttac
     1501 tggaaaggcg gcacggtcag cgtctacggc tacggcagcg gtatcatccc gcgcttttcc
     1561 gaggtcgcgc aggcgttcga ggagtcaaag gaatttcaca ccctgcgcgt gcagccgccg
     1621 gccggcaact actacaccac cgacatgctg cgccagctcg gcgacagctg ggaaaaatac
     1681 ggctcgggac tggtcacttt ccacggccag accggcaaca tcatgttcat cggcacctcg
     1741 accgagaaca cgcagcattt cttcgacgaa atcaacgatt acggctggga tctcggcggc
     1801 gccggtccct gcgtgcgcac cggcatgtct tgcgtcggtt ccggacgctg cgaacagtcc
     1861 tgcgccgacg agatgcgcat tcaccgtacg ctgctgaaca acttcaccga cgacgtccac
     1921 cgccccgcac tgccctacaa gttcaagttc aaggtatcgg gctgtggcaa tgattgcatg
     1981 aactccatcg agcgctcgga tttcgccctc atcggcacct ggcgtgacga catgaaggtc
     2041 gaccaggagg cgttcaagga atacgtcgcc aagaaaggcc gtcaacacac catcgacaac
     2101 atcatcacgc gctgcccgac caaggcattg tcgctcaacg acgacgacac cctcgaggtc
     2161 gacaaccgca actgcgtgcg ctgtatgcat tgcctcaacg tggtgcccaa ggcgctgtcg
     2221 ccaggcgacg acaagggtgt cagcatcctg atcggcggca agcgcacgct gaagatcggc
     2281 gacctgatgg gcaccgttat cgtgccgttc atgaagctcg aggacgagga agacttcgag
     2341 gcgctgaccg aactcgccga ggaagtcatc gacttctggg ccgagaacgg actcgagcac
     2401 gagcgttgcg gcgaaatggt cgaacgcatc ggcctggtca actttctcga gggtatcggt
     2461 gtcgaagtcg acccgaacat gatttcggaa acccggtcca gctcctatgt acgcatggac
     2521 gactgggata tcgaggccaa gaagtggttc gaccgcaagg ccgagcataa gaaggcgtcc
     2581 tagtccggcg ccgcagttag caagttatcc attcagcact gagagcagga gcccgataaa
     2641 tggaaggcaa acgtaccccg atcgaatccg gctgtcccga cggtttccag tacatgcatc
     2701 cgttgatgcg caagaattac ggcaattggg cgtatcacga ggatccccgc cccggcgtac
     2761 tcaagcacgt ctcctggagc ggcgacgcga tccataccgt gcgcgccggc acgcagcgca
     2821 tccttgacgt gttcacgctg cgcaaactgt gcgatatcgg tgaccagtac gccgacggtt
     2881 acgtgcgctt cacgattcgc agcaacatcg aatacatggt ggccgacgag agcaaggtcg
     2941 cgccgctgat cgaggcgctg gaaaaggaag gtttcgtcgt cggtggcacc aagaactcgg
     3001 tggcgatgat ttcccatact caaggctggc tgcactgcga cataccgggc accgatgcat
     3061 cgggcgtggt caagtcgatg atggacgagc tgatcgacga attcaaaaac tggaacatgc
     3121 ccaaccgcgt gcacatcacg acttcctgct gccagatcaa ctgcggcggc cagggtgaca
     3181 tcgcgatcaa cattcagcac accaagccgc cgaagatcaa ccacgacctg gtcgccaata
     3241 tctgcgagcg cccgtcggta gtcgcgcgct gcccggtcgc cgcgatccgc ccggcgctgg
     3301 tcaacggcaa gccgtcgctc gaagtcgacg aaaagaaatg tatttgctgc ggcgcctgct
     3361 acccgccctg cacgccgatg caaatcaacg accctgagca caccaagctc gccatctggg
     3421 tcggcggcaa ccattccaac gcgcgcggca aaccgacttt ccagaagctg gtcgccgcgg
     3481 gaattccgaa caatccgccg cgctggccgg aggcgaccgc agtggtcaag aagatcctgc
     3541 agacctacaa ggacaacgcc cgcgactggg aacggctcaa cgactggatc gagcgcatcg
     3601 gctggccacg gttcttcgag ctcaccgagc tgccgtttac caagtaccac gtcgacaact
     3661 ggcgcggcgc ccgccgtacg ctgaatgcat cgacccatat tcgcttttaa ctgaggctgt
     3721 ggtttgaaat tcggaatcct ggtcaacgag ggcccgtaca cgcatcaggc gtcggacacg
     3781 gcctatcact ttaccgccgc ggcgctgaag gccggtcacg aggtcgtgcg ggtgtttttc
     3841 taccacgacg gcgtcaacaa cggcacccgc ctcggcgtgc cgccgcagga cgaccgcaat
     3901 atcaccgagc gctggtccga gctcgccaaa gactacgatg tcgagctgat cctgtgcgtc
     3961 gccgcggcgc agcgccgcgg cctgctcgat gcggacgagg ccaagcgtgc cggcaaggac
     4021 gtcgataaca tggcgccggg tttccagatt tccggcctcg gccaattgat cgacgccggc
     4081 attcaggccg accggctcgt ggtattcggt gactagccat ggcgactaaa aagttcctct
     4141 atatcaaccg caagcccccg catggctcga tctatgcgct ggagtcgctg gaagtggtgc
     4201 tgatcggcgc cgccttcgag caggatgtca gcctcgcctt cgtcgacgac ggcgtatacc
     4261 agctgctgcg taatcaggat accggcgata tcggcacgaa aaacttttcc ccgacttatc
     4321 gcgcgctcgg cgactacgaa gtcagcaaga tctacgtcga acgcgaatcg ctcgagttac
     4381 gcgggctgag caaggacgat ctgatggagc tgacctacga agacgaagac gatgactggg
     4441 ctgaaaaaga ctcgattcgc atcgtcgaca gcgaagaact cggggccatc atcgagcagc
     4501 aggacgtgct gctgaacttt tagcgaggtc gcgatgccgg tactacatat tatcaacaag
     4561 tcgccctacg aacgcggttc gctcgacacc tgcctgcgac tggcgcagga cgatgcggcg
     4621 atcctgctga tcgaggacgg cgtctatgcg ctgcgcaaca acgcggcgac agccgaaaag
     4681 ctgcgggcag cgctcggaaa acacaagatt tacgcgctgg aaccggacct gaaggccagg
     4741 ggcctggccc cggaaagcgc gctagagggc gtctcgctgg tggattacga cggcttcgtc
     4801 gaactggcga ccgaatacga caagctgcag tcgtggcttt gaacatcgca acacattcaa
     4861 ttttatagag gttagaaatg gcgattgaac tgggtggtaa aacttacgaa acggacgaag
     4921 aaggttacct cgtcaacctg tccgactgga atgacgagct ggccgaggaa atggccaagg
     4981 ccgacggcgc cgagctcggc gaaaatcact gggaagtgat caatttcctg cgtgaatact
     5041 acgaggaata ccagatcgcg ccggcggtac gcgtactgac caaggcggtt ggcaagaagc
     5101 tgggcaagga caagggcacc agcaagtacc tttacgaact gttcccgtac gggccggcca
     5161 agcaagcctg caaatacgcg ggtctgccga agccgaccgg ctgcgtttag gaaggtctgc
     5221 agtctgcacc ggccggtttt gggccggctg gtctttatcg ggatgtgcgg ttctccttat
     5281 gagtttacgg gcaaagtttt caaaactgtt ttcgacgggc ccaaaaccta tcccggccct
     5341 ggctggtcca gatacgcctg aactggccgg cgcgcgccgc gttccttcgg gccatagctt
     5401 tggctatggc tctcagtcca gcccggcgtt cagccgcatc tgtcctgcgc caggactcgg
     5461 gatgccggca cagcctgcag accggactcc cggaacacga atcggaacag gctttcagca
     5521 aaagatgcgg acgaagttgt catgtcgttg ttgacccttg cctatacgct gctgttctac
     5581 gctgcgagcc tcgtgttgct agcgggactg gcctttcgaa tccgcctcta cgcgcgcacg
     5641 ccgactccgt tgcgcattcc gaccacaccg gcgccgacca cctcggccgg cgtggtgcga
     5701 cggatgtttt tcgaagtcgt gctgttcaac agcctgttca aggccagcaa atggacctgg
     5761 ctattcggct ggctgatgca tttcgcgctg ctgctggtgc tgctgcgcca tctacgttat
     5821 ttcaccgatc cggtctggtc gtgggtggca ttcctgcaac ccttcggctt ctacgccggc
     5881 ttcgcgatgc tcgccggcct cggcggcctg tgggcgcgtc gtctgctggt ggaccgtgtg
     5941 cgctatattt cggcaccgtc cgaccacctg atgctggccc tgctggccgg catcgtgctc
     6001 accgggctcg gcatgtccta cgtggcgccg accgacatcg tcgcggtgaa ggcgtttttt
     6061 ctcggcctga tgtatttcga ctggcagccg atgccggccg actggatgct caacctgcac
     6121 ctgttgctgg tggcgctgtt gatgctggtg tttccgttca gcaagctgct gcacgcaccg
     6181 ggtgtatttt tcagcccgac ccgcaaccag gtcgataacc cgcgcgagca ccgccacctg
     6241 ccgacgcgcg gtccggcggc cgccgattcc ggcaactgaa atcgagcagg cgcatcatgg
     6301 ctgaattcga aaccccggca ctgaaaccct ggcccgagat cccgccgatc gaaatcggcg
     6361 cgatggcgca cagcaagccg ttcgtggcca agcaagagca ccagcagaaa atcggtttcc
     6421 cgggcgaact ggtcgacaac tggcaacagg tcgcgatcga caagctcggt gaactggtca
     6481 ataactcgcg cggcctgcgc gtgttcctcg acagctgcgt caag
//
LOCUS       DATCZV010000255         5156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_125698, whole genome shotgun sequence.
ACCESSION   DATCZV010000255 DATCZV010000000
VERSION     DATCZV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125698"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1335)
                     /locus_tag="VIW27_05650"
     CDS             complement(103..1335)
                     /locus_tag="VIW27_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739188.1"
                     /translation="MSDTHSNPLGHGDRELTAARRFLAIFAAFVLLVAALVASVNLVA
                     YRYMLRDANQDVVQLLSGWGRMYKPILFDELQPEIAVYGASWARDAFDPIATGQLLGR
                     RVFNHAVSGGTAYETRRFGESSLADSKLQAAIVNLDTFYRSELVARNRYGFDESILDV
                     DPDGKPNRWVGLRRNYSLALTGWAAGANLELISALHARDAGAARENYLEAYQSADLSS
                     RRSRMETARQQIFPRADEPSGSHDDAEPPPLAAEPAELGKMIDGFCRSGIDIHAYFTP
                     SHARQLHCDPEATKELAALAFLRRKQAACDARIHYYDFGYPNAVTLEGLLQPVKSSRY
                     YRPDNHPRPTVGLLMAARMFGTEFPLGTPALIERDFGVDLLSHENPEGWLLERAARCT
                     GNWGDSGYEEFARALAAD"
     gene            complement(1345..2853)
                     /locus_tag="VIW27_05655"
     CDS             complement(1345..2853)
                     /locus_tag="VIW27_05655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY5739189.1"
                     /translation="MVFASHSFLAFLAIVVFAYWLVGRHDERLGKPLLIVASFVFYGY
                     WIPTYLLLLSASIVFNHVVVRLLLGDIGAGWRRTLTAIGIAANLALIGFYKYAAFVVE
                     SLNWSLDTGFDVPDIVLPIGISFFTFQQISLLVDTYRGRVAPFGFWDHTLFISFFPQL
                     IAGPIVRQHDMLPQLRARRDWRLRADYLAIGLGLFAFGLFKKTALIDPVVPYLDLVYR
                     AAADGDALGFIDGWAAGIGYGFQVYFDFSAYSDMAIGLGFMFGLRLPINFFSPYKADS
                     IRSFWRRWHISLSRFLRDYLFIPLGGSRHGLARTVAALTVTMALGGLWHGAGWTFVIW
                     GLLHGAYLSINHLWRALVVARLPGLAGRVAASRVLSLLARWLSTLLTFLAVSFAWVFF
                     RSPDFDSAMRLATAMLGFSGWHEVVLLTRGIVPMFPLYLLIVWLLPNTMEIFQATRAA
                     LHVDEYRDPERRPLARWLRFGLSTRWAATTALVFVCAWFALSNLSPFIYFQF"
     gene            complement(3020..3943)
                     /locus_tag="VIW27_05660"
     CDS             complement(3020..3943)
                     /locus_tag="VIW27_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5739190.1"
                     /translation="MDFNTLLAGDSRILGDGSMYELLRRSPEVDFDPHIAHAGLIYDR
                     ASRAALERVMRAYIEVGVSRRLPMALTTTTWRANRERVAASAFAGRAVNEDNARFLLQ
                     LRDEYSGPEQPLLVGGVIGPQGDAYKPGEAPGTEQARQFHAWQIDALAGSGVDYLQAA
                     TVPALPEARGIARAMAATGLPYIISFVVEKSGNLLDGHSLSETIDEIDAILPGSSRYA
                     INCVHPSVLLQALDRNPRIGNLIVAFAGNTSARSVDELDGLEELDTEDPDIFADAHRR
                     LLDDYDIRIVGGCCGTSPAHMQAIATRLLNS"
     gene            4063..4695
                     /locus_tag="VIW27_05665"
     CDS             4063..4695
                     /locus_tag="VIW27_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY5739191.1"
                     /translation="MQRIDWYFDFISPFSYLASASLERLPGDIELRARPILFAALLQH
                     WDTRGPAEIAPMRRFTFRHVCWLAERDGIELELPPQHPFNPLKLLRLCLLLDADLAIA
                     RRLFRFVWAEGRSADDPDHWQALLDELGVPDADRRIATAEIKDGLRRNTEQALEAGVF
                     GVPGFAVDGEIFWGYDAMDFLCAYLEEPAVLRSPGMRAADALPEGPQRRR"
     gene            complement(4713..>5156)
                     /locus_tag="VIW27_05670"
     CDS             complement(4713..>5156)
                     /locus_tag="VIW27_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY5739192.1"
                     /translation="ARVLIEAGADVNAMDDISDSPYLYAGAEGPLEILRMTLEHGADL
                     ASVNRYGGTALIPAAHHGHVDIVRELLGTGIDIDHVNYLGWTALLEAVILADGGPVHT
                     EIVRLLLEAGADAEIADNDGVTPLVHARRRGYTAMVELLHAAGND"
BASE COUNT          975 a         1583 c         1758 g          840 t
ORIGIN      
        1 ctgatcttcg cgtagttgac cagcttgtcc tcgaggggct gcgagtcggt cacgaagttg
       61 gtcatgcgcc ggcgtgcgga cggggatcga tcgcggcgac ggttaatcgg cggccagcgc
      121 ccgggcgaac tcttcgtaac ccgagtctcc ccagttaccc gtgcagcggg cggcgcgctc
      181 cagcagccag ccctcgggat tttcgtggga cagcagatca acgccgaaat cccgctcgat
      241 cagcgccggc gtgccgaggg ggaattcggt gccgaacatg cgtgccgcca tcagcaggcc
      301 gacggtgggc cgcggatggt tgtcggggcg gtagtagcgg ctcgatttca ccggctgcag
      361 caagccttcc agggttaccg cgttgggata gccgaaatcg taatagtgaa tgcgcgcgtc
      421 gcaggcggcc tgcttgcggc gcaggaaagc gagggcggcc agctctttgg tggcctcggg
      481 atcacagtgg agctggcgcg catgcgatgg cgtgaagtag gcatggatat cgatgccact
      541 gcggcaaaag ccgtcgatca tcttgcccag ctcggccggt tcggcggcca gcggcggcgg
      601 ctccgcatcg tcatgcgagc cgctgggctc gtcggcgcgc ggaaaaattt gctgccgcgc
      661 ggtctccatc ctgctgcggc ggctgctcag gtcggcgctc tggtaggctt ccaggtaatt
      721 ctcccgggcg gcgccggcat cgcgcgcgtg cagcgccgag atcagctcca ggttggcgcc
      781 cgcggcccag ccggtcagcg ccagcgaata gttacggcgc agcccgaccc agcggttcgg
      841 cttgccgtcg ggatcgacgt cgagtatcga ttcgtcgaag ccgtaacgat tcctggctac
      901 cagctccgaa cggtaaaagg tatcgaggtt gacgatcgcc gcctgcaatt tcgagtccgc
      961 gagcgacgac tcgccgaaac ggcgggtttc gtaggcggtg ccgccggaga ccgcgtgatt
     1021 gaatacccgc cggcccagca gttggccggt ggcgatcggg tcgaacgcgt cgcgcgccca
     1081 ggaggcgccg tagaccgcga tttcgggttg cagttcatcg aacaggatcg gcttatacat
     1141 gcgtccccaa ccggacagca gttgcaccac gtcctggttg gcatcgcgca gcatgtagcg
     1201 gtaggcgacc aggttgacgc ttgccacgag ggcggcgacc agcagcacga aggcggcgaa
     1261 aatagccaga aagcggcgcg ccgcggtgag ttcgcgatca ccatggccga gcgggttcga
     1321 gtgtgtgtcg ctcatcgcag tcgatcagaa ctggaagtag atgaacgggc tcaggttcga
     1381 cagcgcgaac caggcgcaga cgaagaccag cgcggtggtc gccgcccagc gggtcgacag
     1441 cccgaagcgc agccagcgag ccagcggccg gcgctcgggg tcgcggtact cgtcgacgtg
     1501 cagcgcggcg cgcgtcgcct gaaatatttc catcgtgttc ggcaacagcc agacgatcag
     1561 caggtagagc ggaaacatcg gcacgatgcc gcgggtcagt aataccacct cgtgccagcc
     1621 ggagaaaccc agcatcgcgg tcgccagccg catcgcactg tcgaagtccg gcgagcggaa
     1681 aaatacccag gcgaagctga ccgcaagaaa ggtcagcagc gtcgacagcc agcgcgccag
     1741 cagcgacagc acgcgcgagg cggccacgcg gccggcgaga ccgggcaggc gcgccaccac
     1801 cagcgcacgc cataaatggt tgatcgacag ataggcaccg tgtagcagac cccagatgac
     1861 gaaggtccag ccggcgccat gccagagacc gccgagcgcc atggtgacgg ttagcgcggc
     1921 aaccgtgcgc gccagcccgt ggcggctgcc accgagcgga atgaacaggt agtcgcgcag
     1981 gaaacgcgac aggctgatat gccagcgccg ccagaagctg cgaatactgt cggccttgta
     2041 aggcgaaaag aaattgatcg gcagccgcag cccgaacatg aagccgaggc cgatcgccat
     2101 gtcggagtaa gcagaaaaat cgaagtaaac ctggaaaccg tagccgatgc cggccgccca
     2161 gccgtcgatg aatccaagcg cgtcgccgtc ggccgcggcg cggtagacca gatcgaggta
     2221 gggcaccacg gggtcgatga gtgcggtctt cttgaacagg ccgaaggcga atagcccgag
     2281 gccgatggcc aggtagtcgg cgcgtagccg ccagtcgcgc cttgctcgca actgcggcag
     2341 catgtcgtgc tggcgcacga tcgggccggc gatcagctgc ggaaagaagc tgatgaacag
     2401 cgtatggtcc cagaaaccga atggcgcgac gcgtcccctg taggtatcga ccagcaggct
     2461 gatttgctgg aaggtaaaaa acgagatacc gattggcagc acgatgtcgg gaacgtcgaa
     2521 accggtgtcg aggctccagt tgagcgattc gacgacgaac gcagcgtact tgtagaaacc
     2581 gatcagcgcc aggttggcgg caatgccgat ggcggtcagc gtacgtcgcc agccagcgcc
     2641 gatatcgccg agcagcagac ggacgacgac gtggttgaag acgatcgaag cgctgagcag
     2701 cagcaggtag gtcggaatcc agtaaccgta gaaaacgaac gaggcgacga tcagcagcgg
     2761 tttgccgagg cgctcgtcgt ggcggccgac gagccagtag gcaaatacca cgatggccag
     2821 aaaggccaga aaggaatgcg atgcgaatac catggggctc cggcatccgg cgcggggccg
     2881 gagttgtact cagtgcgctt gcgtcgacgc cgcaatgcta ggcggtgtat atcaggattc
     2941 cgcagcgctg taaaatcacc gcggccggta gcccgcaagt cccgcggcaa tccggatcgc
     3001 ggccgccgcg aatcgccggt taactgttta gcagtcgtgt cgcgatggct tgcatgtgcg
     3061 cagggctggt gccgcagcaa ccgccgacga ttcgaatgtc gtaatcatcg agcaggcgac
     3121 ggtgggcatc ggcgaagata tcgggatcct cggtatcgag ttcttcgaga ccgtcgagtt
     3181 cgtcgacgct gcgcgccgaa gtattgccgg caaaggcgac gatcagattg ccgatgcgcg
     3241 ggttacgatc gagcgcctgc agcaataccg acgggtgtac gcaattgatc gcgtaacggc
     3301 tgctgccggg caggatcgca tcgatctcgt cgatagtttc gctgagcgag tgcccatcga
     3361 gcaggttgcc ggacttctcg accacgaaac tgatgatgta gggcaggccg gtagcggcca
     3421 tcgcgcgggc aataccgcgg gcctcgggca gtgcgggcac ggttgccgcc tgcaggtaat
     3481 cgacgccgct gccggcgagc gcgtcgatct gccaggcgtg gaactgtcgc gcttgctcgg
     3541 tgcctggcgc ttcgccgggc ttgtaggcgt cgccttgcgg gccgatgaca ccgccgacca
     3601 gcagtggctg ttcgggtccg ctgtactcgt cgcgcagttg cagcaggaag cgggcattat
     3661 cctcgttgac cgcgcgcccg gcgaacgccg atgcggcgac acgctcgcga ttggcgcgcc
     3721 aggtagtggt cgtcaacgcc atcggcaagc gccgcgacac gccgacctcg atgtaggcgc
     3781 gcatcacgcg ctcgagcgcg gcccgggagg ctcggtcgta gatcaggccg gcatgggcga
     3841 tatgcgggtc gaagtcgacc tcgggactgc gtctgagcag ttcatacatc gagccgtcgc
     3901 cgagaatacg gctgtcgccg gccagcaggg tgttgaaatc catggcgaat cctccgcaaa
     3961 gattcccatc gatcggcaaa tcttaacacg ccgatgcccg ctttcgagat aaactcgatg
     4021 cgctcgcaat tatttgttac cgaccgccgg aggttttaaa agatgcaacg gatcgactgg
     4081 tacttcgact ttatctcgcc gttttcctac ctcgccagtg ccagcctcga gcggctgccc
     4141 ggcgacatcg agctgcgcgc gcggccgatc ctgttcgccg ccctgctgca gcactgggat
     4201 actcgcggac cggcggaaat cgcgccgatg cggcgcttca cgtttcgcca tgtttgctgg
     4261 cttgccgagc gcgacggcat cgagctcgaa ttgccgccgc aacacccgtt caacccgctg
     4321 aagctgctgc gcctgtgcct gttgctggac gccgacctgg cgattgcccg gcgcctgttt
     4381 cgtttcgtct gggccgaggg acgctcggcc gacgatcccg atcactggca ggcactgctc
     4441 gacgagctcg gcgtgcccga tgccgatcgg cgcatcgcga ccgcggaaat caaggacggc
     4501 ctgcggcgca ataccgagca ggcactcgag gccggcgttt tcggcgtacc gggattcgcc
     4561 gtcgacggcg agatcttctg gggctacgac gcgatggatt tcctgtgtgc ctacctcgag
     4621 gagccggccg tgctgcgatc gccgggcatg cgcgccgccg acgcgctgcc cgagggaccg
     4681 caacggcggc gataaccgcg gggcagtcgg gtctagtcgt tgcctgccgc gtgcagcagc
     4741 tcgaccatgg cggtgtaacc acgccgccgc gcatgtacca gtggtgtgac accgtcgttg
     4801 tcggcgattt cggcatcggc gccggcctcg agcagcaggc gcacgatttc ggtatgtacc
     4861 ggaccgccgt cggcgaggat cacggcttcg agcagcgcgg tccagcctag atagttgacg
     4921 tggtcgatgt cgatgccggt gccaagcagc tcgcgcacga tatcgacgtg accgtggtgg
     4981 gccgccggaa tcagcgcggt gccgccatag cggttgacgc tggcgagatc ggcgccgtgc
     5041 tcgagcgtca tgcgcaggat ttcgagcggg ccctcggcac ccgcgtacag gtaaggcgaa
     5101 tccgagatat catccatggc gttgacatcg gcaccggcct cgatcagcac gcgcgc
//
LOCUS       DATCZV010000256         2041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_496496, whole genome shotgun sequence.
ACCESSION   DATCZV010000256 DATCZV010000000
VERSION     DATCZV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496496"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VIW27_05675"
     CDS             complement(<1..284)
                     /locus_tag="VIW27_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009809925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY5739193.1"
                     /translation="MKLDILLALCSFAFVASVTPGPNNLMLMASGANFGIRRTLPHLF
                     GIVFGFSAMVILVGVGLMQIFDVYPLSYELLRIFSIVYLGYLAWKIAGSTS"
     gene            357..809
                     /locus_tag="VIW27_05680"
     CDS             357..809
                     /locus_tag="VIW27_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5739194.1"
                     /translation="MKAFDRYNSRILQELQRDGRISNIELAERIGLSASACLRRVQEL
                     EKSGVIRGYRAVLDNERRGIGFIAYITVGLSDHTLPAQESFEAAMRAAPEVRECHNTT
                     GIVEYLVRAELPDIAAYKAFHLEVFGSLPQVNAITTYVVLDSPKDERG"
     gene            814..1899
                     /locus_tag="VIW27_05685"
     CDS             814..1899
                     /locus_tag="VIW27_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HEY5739195.1"
                     /translation="MAQLPDTMKAVVLTGHGGLDKLEYRDMPVPLPGAGEVLIEVHAC
                     GMNNTDVWVREGAYGTDDDPAAVASWRRGKPTLTFPRIQGADTVGLIAAVGDGVDAAR
                     VGERIMVDFSIYNTDGDSLADIDYIGHGADGGYAEYCVVPADHAHAVATPMSDAELAT
                     FCCAYLTGEHMLDRARVAAGERVLISGAAGGVGGGLIQLCRARGAIPYAITSSDKYDA
                     VAALGAEGVALRDRGDWVAQVEQLLDGEPLDVVADVVAGPLFKDLINLLRPEGRYTTA
                     GAFAGAMVELDLRTIYLKHLEIHGSSQGTRQAFRRLIGYIESGKLEANLFATYPLSEF
                     HRAQTDFMAKTYVGKLVVMPDRFHHPP"
BASE COUNT          428 a          602 c          624 g          387 t
ORIGIN      
        1 gacgtcgacc cggcgatctt ccacgccagg taaccgagat agacgatgct gaagatgcgc
       61 agcagttcat agctcaacgg gtagacgtcg aatatctgca tcaggccgac accgacgagg
      121 atgaccatcg ccgagaagcc gaacacgatg ccgaacaggt gcggcagcgt gcggcggata
      181 ccgaagttcg cccccgacgc catcagcatc aggttgttcg ggccgggcgt caccgaggca
      241 acgaaggcga agctgcagag cgcaagcaga atatcgagct tcattgcgca atattaagtc
      301 tttttactta atattttatt gcttataatg ctcaaatatt aaatattaaa caatttatga
      361 aagcttttga tagatataac agccgaatat tgcaggaact tcagcgcgat ggccggatca
      421 gcaatatcga gctggccgag cgcatcggac tgtcggcttc ggcatgcctg cgccgtgttc
      481 aggagctgga aaagtcgggt gtcattcgcg gttaccgcgc ggtgctcgac aatgagcggc
      541 gcggtatcgg cttcatcgcc tatatcaccg tcggcctgtc ggaccatacg ctgccggcac
      601 aggaatcctt cgaggcggcg atgcgggcag cgcccgaagt gcgggaatgt cataatacca
      661 ccggcatcgt cgagtacctg gtaagggccg agttgcccga catcgcggcc tacaaggcat
      721 ttcatctcga ggtattcgga tccttgcccc aggtcaacgc gattaccacc tacgtggtac
      781 tggattcacc caaagacgag aggggttaaa gatatggcgc aattgcccga caccatgaag
      841 gcggtcgtgt tgaccggtca cggcgggctc gacaagctcg agtaccgcga catgccggtt
      901 ccgcttccgg gcgcaggcga agtgctgatc gaggtacatg cctgcggcat gaacaatacc
      961 gacgtctggg tccgcgaggg cgcttacggt accgacgatg accccgccgc agtagcttcg
     1021 tggcggcgcg gcaagccgac ccttactttt ccgcgcatcc agggggccga tacggtcggc
     1081 ttgatcgccg cggtcggcga cggcgtcgac gcggcgcgag tcggcgaacg catcatggtc
     1141 gatttcagca tttacaacac cgacggcgac agcctcgccg atatcgacta catcggccac
     1201 ggcgccgacg gcggttacgc cgagtactgc gtggtgccgg ccgatcatgc ccacgccgtg
     1261 gctacgccga tgagcgatgc cgaactggcg actttttgct gtgcttatct gaccggcgaa
     1321 catatgctcg accgggcgcg ggtggctgcc ggcgaacggg tgctgatcag cggcgcggcc
     1381 ggcggcgtcg gcggtggcct gatccagctg tgccgggcgc gaggcgcaat accttacgcg
     1441 atcaccagca gcgacaagta cgacgcggtc gcggcgctgg gcgccgaggg ggtggcgttg
     1501 cgcgaccgcg gcgactgggt cgcgcaagtc gagcaactgc tcgacggcga gccgctcgac
     1561 gtggttgccg atgtcgtcgc cgggccactg ttcaaggact tgatcaacct gttgcgcccc
     1621 gagggccgtt acacgacggc cggcgcgttt gccggcgcga tggtcgaact cgacctgcgc
     1681 acgatctatc tgaaacacct cgagattcac ggttcgtcgc agggcacgcg ccaggcattc
     1741 cggcgcctga tcggctatat cgaatccggc aagctcgaag ccaacctgtt cgcgacctat
     1801 ccgctgtccg aattccaccg cgcgcaaacc gatttcatgg ccaaaactta cgtcggcaaa
     1861 ctggtggtca tgccggaccg cttccaccac cccccctgac cctccaatta atttgggtca
     1921 gatccaaaat aaaagctgcc ctttaatttg ggtcagatcc aaattaaaga cagcgagtta
     1981 attcagatct gacccaaatt tatgccgggg cggggttggc gagtgacagc attgcgtgca
     2041 g
//
LOCUS       DATCZV010000257         3880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_708654, whole genome shotgun sequence.
ACCESSION   DATCZV010000257 DATCZV010000000
VERSION     DATCZV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708654"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VIW27_05690"
     CDS             <1..905
                     /locus_tag="VIW27_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5739196.1"
                     /translation="YATSRYLFGFDLLLALFVGGTLTATSIGVTVRILRDVNRQNSRE
                     GQIVLGAAVIDDILGVLLLALLYEFSQTGDLSAANLLRLVLFIGVFFALAPLAAKLIS
                     TLIGHYHRASQMPGLVATLIVSLVMVFAWLAHIVGAPELLGGFTAGLALSRRFFLPFG
                     AALQADPEFSSHVHEQMKPIIQLFTPIFFATVGLSLDLSSVDWGSLFFWIFSISLAAV
                     AVATKILGALLLREPLARRVVIGMSMVPRGEVGLVFAELGRQTGLLDQQVFAIVVIVI
                     AYTTLFTPFWLRLFYRRCGHLLAD"
     gene            966..1688
                     /locus_tag="VIW27_05695"
     CDS             966..1688
                     /locus_tag="VIW27_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5739197.1"
                     /translation="MSNNPQSIILVGASGGIGGGLLDALLQRYPQASIHASYHRHPGR
                     LEDPRVHWQCLDVRDEQAIADWAANFERVDWLLNCVGFLHSDAGRPEKSIHALQGDFL
                     LENIRVNTLPTLLLAKHFDAAFKASSAPLLATVSARVGSIEDNRLGGWYSYRMSKAAL
                     NMALKTLAIEWRHRHPRGCVAALHPGTNDTALSKPYQARVAPEALFDPADTAVSFVSL
                     LAGLEASDSGNFWAWNGERLSW"
     gene            1711..2256
                     /locus_tag="VIW27_05700"
     CDS             1711..2256
                     /locus_tag="VIW27_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY5739198.1"
                     /translation="MKLLRFIPAIPLLLFANGTLAACSDLIDFQAQKLRSDETIDFCS
                     NFEAKVLLVVNTASHCGFTPQFEGLETLYRKYREQGLEIVGFPSNDFFQEASSEAKTA
                     EVCYINYGVTFTMLSPSAVRGSDANPLFRRLAQATGNAPSWNFNKYLIDRQGRSVKHY
                     GSRVEPLHSQLEQDIVESLGR"
     gene            2263..2682
                     /locus_tag="VIW27_05705"
     CDS             2263..2682
                     /locus_tag="VIW27_05705"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEY5739199.1"
                     /translation="MDSSTRVTHSLGYAGLIPFVVPALLVLGGIGQAGLWQSIAGAYA
                     FGIIAFLAGSWWGMARSSGAPALLVLSNLYFLLALACYLLAPQSWPLAAALLLLAIFL
                     TEQNTALMPTFADSYRKLRARLTLIAGGSMLAVFIGA"
     gene            2758..3180
                     /gene="folX"
                     /locus_tag="VIW27_05710"
     CDS             2758..3180
                     /gene="folX"
                     /locus_tag="VIW27_05710"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007623493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HEY5739200.1"
                     /translation="MKPVHSITAPAATLPISEARINISNLRLRTYIGFNPEEKTKQQD
                     VIVNAEIHYRANPVFDSDDQRDALNYKLITKRMIRHIENGRFQLLEKLTADLLAIACE
                     SERVSFAEVRVDKPHALRFADSVSVTLSADRRRPGRRD"
     gene            3247..>3880
                     /gene="folE"
                     /locus_tag="VIW27_05715"
     CDS             3247..>3880
                     /gene="folE"
                     /locus_tag="VIW27_05715"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY5739201.1"
                     /translation="MTSSIAPPEAVIPSPFTAEADRVRQALVERGLETPLLPHQYERD
                     EKYQRIRRSFSDIARTLGLDLSDDSLRETPDRIARMYVDEIFAGLDYLNFPKITVIDN
                     KMGVEEMVKVSDIAVTSTCEHHFVTIDGTARVAYIPRDKIIGLSKINRIVRFFAQRPQ
                     VQERLTQQLLVALQTLLETKDVAVSVDATHYCVKARGVMDSNSRTQTTALG"
BASE COUNT          713 a         1282 c         1146 g          739 t
ORIGIN      
        1 gatatgccac gagccgttac ctctttgggt tcgacctgtt gctggcgctg ttcgtcggcg
       61 gcacgctgac cgcgaccagc atcggcgtga ccgtccgcat cctgcgcgac gtcaatcgac
      121 agaacagccg cgagggtcag atcgtgctgg gcgccgcggt gatcgacgac atattgggcg
      181 ttctgctgct cgcgctgctt tacgagtttt cccagaccgg cgatctgagc gccgcgaacc
      241 tgctgcggct cgtgctgttt atcggcgtgt tttttgcgtt ggccccgctc gcggcgaaac
      301 tgatctcgac gctcatcggc cactatcatc gcgcgagcca gatgcccggc ctggtcgcga
      361 cgctgatcgt gtcgctggtg atggtgtttg cttggctcgc acacattgtc ggcgccccgg
      421 aacttttggg tggctttacg gcgggtcttg cgctgtcgcg gcgtttcttc ctgccgttcg
      481 gcgcggcgct gcaagcggac cccgagtttt cctcacacgt gcacgaacag atgaagccca
      541 tcatccagct gttcacgccg attttcttcg ccacggtagg actatcgctg gacctcagca
      601 gtgtcgactg gggctcgctg tttttctgga ttttctcgat cagcctcgca gcagtcgcag
      661 tcgccaccaa gatactggga gccttgttgt tgcgcgaacc gttggcgcgg cgcgtggtca
      721 tcggcatgtc gatggtgccg cgcggcgagg tcgggctggt atttgccgag ctcggccggc
      781 aaaccggcct gctcgaccag caggtgttcg ccatcgtggt catcgtcatc gcctacacga
      841 cgctgttcac gccattctgg ctgcgcctgt tctaccgccg ctgcgggcat ttgctggcgg
      901 actgatccgc ggaccgccgg gacgcgtaac taacctggtt aggattcata aacggcaaag
      961 gcaacgtgag caacaacccg cagagcatca tcctggtcgg cgccagcggc ggcatcggcg
     1021 gcggtctgct ggatgcgctg ttgcagcgct acccgcaagc ctcgatccac gccagctatc
     1081 atcgccaccc cggccggctc gaagatccgc gcgtccactg gcaatgcctc gatgtccgcg
     1141 acgagcaagc gattgccgac tgggccgcca atttcgaacg cgtcgactgg ctgctcaact
     1201 gcgtcggctt cctgcactcg gacgcgggcc ggccggaaaa aagcattcac gcattgcagg
     1261 gcgacttcct gctggagaat attcgcgtca ataccctgcc gaccctgctg ctggcaaagc
     1321 atttcgacgc cgcgttcaag gctagctccg cgccgttgct ggccaccgtg tccgcgcgcg
     1381 tcggcagtat cgaggacaac cgcctgggcg gctggtacag ttaccgcatg tcgaaagcgg
     1441 ccctcaacat ggccctcaag accctcgcta tcgagtggcg ccaccggcat ccgcggggct
     1501 gcgtcgcggc gctgcacccg gggaccaacg acaccgcgct gtcgaaaccc taccaggccc
     1561 gcgtcgcacc cgaggcccta ttcgacccgg ccgataccgc ggtcagtttc gtttccttgc
     1621 tggccgggct cgaggcttcc gacagcggca atttctgggc ctggaacggc gaacgccttt
     1681 cctggtgaaa cattcataag aggaccgatc atgaaactgc ttcgcttcat acccgccatc
     1741 ccgctgctgc tgttcgccaa cggcacgctc gccgcctgca gcgatctgat cgatttccag
     1801 gcgcaaaaac tgcgctccga cgaaaccatc gacttttgca gcaatttcga ggccaaggtc
     1861 ctgctggtgg tcaacactgc cagccactgc ggtttcaccc cgcagttcga gggcctggaa
     1921 acgctctatc gcaagtaccg cgagcagggg ctggaaatcg tcggctttcc atccaacgat
     1981 ttcttccagg aggccagcag cgaggccaag actgcggaag tctgctacat aaactacggt
     2041 gtcaccttca cgatgctatc accaagcgcg gtgcgcggca gcgacgccaa cccgctgttt
     2101 cggcgactcg cgcaggcaac cggcaatgca ccgagctgga acttcaacaa gtacctgatc
     2161 gaccgccagg gacgcagcgt caaacactat ggctcgcgcg tcgagccgct gcacagccag
     2221 ctcgagcaag acatcgtcga atcgctgggc aggtagccgc gcatggacag ctcgacacgg
     2281 gttacgcatt cgctgggtta tgcggggctg attccgttcg tcgttccggc actactggtg
     2341 ctcggaggta tcgggcaagc gggactgtgg cagtcgatcg ccggcgccta cgccttcggc
     2401 atcatcgcct ttctcgccgg cagctggtgg ggtatggccc ggtcatcggg tgcaccagcc
     2461 ctgctggtgc tgagcaacct ctatttcctg ctggcgctgg cctgctattt gctcgcgccg
     2521 caatcgtggc cgctggcggc ggcgctgtta ttgctggcga tcttcctgac cgagcaaaac
     2581 acggccttga tgccgacctt tgcagattct tatcgaaaac tgcgcgcccg actgaccctg
     2641 attgccggcg gctcgatgct ggcggtcttt atcggcgcct gacggctgcg ccgcgaagtg
     2701 atccgcaggc cggttcgcgg cgtaagctag ggtgaatcga taaccgacgg acagcgcatg
     2761 aaaccggtcc acagtataac agcaccagcc gcaaccctgc cgatcagcga ggccaggatc
     2821 aatatcagca acctgcgact gcggacctat atcggcttca accccgagga gaagaccaag
     2881 cagcaggacg tgatcgtcaa tgccgaaatc cactatcgcg ccaacccggt gttcgacagc
     2941 gacgaccagc gcgacgcgct gaactacaag ttgattacca agcgcatgat ccggcatatc
     3001 gagaacggcc gtttccagct gctggaaaaa ctgaccgccg acctgctggc gatcgcctgc
     3061 gagtcggaac gcgtcagctt cgccgaggtc cgcgtcgaca agccgcatgc tttgcgcttc
     3121 gccgactcgg tatcggtgac gctgtccgcc gaccgccgcc gcccggggcg gcgtgactga
     3181 ccacgcgaca ccggattcgc tcccggcgct gcaatcgaac cgaccgacac ccgcacagga
     3241 ccagccatga cttccagtat cgcaccgcca gaagccgtta tcccctcacc gtttaccgcc
     3301 gaggcagacc gcgtgcgcca ggcgctcgtc gagcgcggac tcgaaacgcc attgctgccg
     3361 caccagtacg aacgcgacga gaaataccag cgcattcgcc gctcgttcag cgacatcgcc
     3421 cgcacgctgg gcctggacct gagcgacgac agtctcaggg agaccccgga ccgcatcgcc
     3481 cgcatgtacg tcgacgaaat ctttgccggc ctcgattatc tcaactttcc gaaaatcacc
     3541 gtgatcgaca acaagatggg cgtcgaggaa atggtcaagg tcagcgatat cgccgtcacc
     3601 agcacctgcg aacatcactt cgtgaccatc gacggcaccg cccgcgtggc gtatattccc
     3661 cgcgacaaga tcatcggact gtcgaaaatc aaccgtatcg tgcgtttctt cgcgcagcgc
     3721 ccgcaggtgc aggagcggct cacccagcaa ctgctggtcg cgctacagac cctgctggag
     3781 acgaaggacg tggcggtcag cgtcgatgct acccactact gtgtcaaggc gcgcggcgtg
     3841 atggactcca attcgcgcac ccagactacc gcgctcggtg
//
LOCUS       DATCZV010000258        10885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_656054, whole genome shotgun sequence.
ACCESSION   DATCZV010000258 DATCZV010000000
VERSION     DATCZV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656054"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VIW27_05720"
     CDS             <1..201
                     /locus_tag="VIW27_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739202.1"
                     /translation="SLGLYTTRADLEALVAAVRDLAGRRDEILALYDPIGSNGYRHRE
                     FKPAAQDLFDPERSLELYLQAS"
     gene            587..1810
                     /locus_tag="VIW27_05725"
     CDS             587..1810
                     /locus_tag="VIW27_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5739203.1"
                     /translation="METPIDNYLKILAHKDGSDLYLSTGAPACAKFNGVLKPLSQNAY
                     KAGEIEAIANEIMDEEQREEFVHELEMNLAISIPGVGRFRINIFKQRNEVSIVARNIK
                     AEIPKFDDLGLPVVLKEIITAKRGLVLFVGGTGSGKSTSLAALIDHRNSNSAGHIITI
                     EDPVEFVHKHKKSIVNQREVGVDTRTFHNALKNTLRQAPDVILIGEIRERATMEHALE
                     FAETGHLAISTLHANNANQALERIINMFPEERRQQLLLGLAQNIRAIVSQRLVPTTDN
                     KRCAAVEVLLGTKTIQELILKSRLNEIKEIMEKSEGLGMQTFDAALFKLYMSQVISLD
                     EALANADSPNNLRLRIKLANESGPEGDASAADGSEAAAESAFGELSLEEIEEPEDEEP
                     GEDSGIVNILDRKAQ"
     gene            complement(1947..2816)
                     /locus_tag="VIW27_05730"
     CDS             complement(1947..2816)
                     /locus_tag="VIW27_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5739204.1"
                     /translation="MPFLQSWQCERFADEGYLLVENAVDADQVSALRNQFSDWVEASR
                     QQCRPWGKTLDGRARFDLEPGHDKDRPALRRVNAPTDISAEYLAAMRDSRIADYVAEL
                     IGPNVKFHHSKINSKLPGAATAVKWHQDFAFTPHSNDDLVTALLMLDDLSEENGPLQV
                     LPGSHRGAIYDLWHDGEFTGAIDDDLARDCLARSVSCTGTAGTLCLMHTRLLHGSAAN
                     RSPRPRTLFIVVYSAEDAVPCAPSPVPCRHQGEVVRGVATGRVRATGYDILLPQMPRS
                     ASFFDQQASSGRD"
     gene            2970..4175
                     /locus_tag="VIW27_05735"
     CDS             2970..4175
                     /locus_tag="VIW27_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015268206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY5739205.1"
                     /translation="MTDSPHDDMGLQTLAIHGGESPDPSTGASSPNLVMSSTYVVDEE
                     VSFSANNLAAETPFVYTRWDNPTSRQLQSKLSLLENAEACLAFASGMAASCAVLLAHL
                     DPGDHLVISDTNYPGTAEFVRNTLTRLGVEVSPVDTSQNGAIEAALRDNTRMLWLETP
                     SNPLLKIADIRAAAELAHARGALLVVDSTFATPIATRPLELGADLVVHSLTKYIGGHG
                     DAMGGAVCGNQDLLGALRGEALVHYGGVISPFNAWLILRGMATLPLRMRCHQQNALAV
                     ADFLEAHALVERVYYPGLESHPQHALASSQMRNFSGMISFRSAQPKHVAARMMQELRV
                     IHYAVSLGHHRSLIYLMQTDDLVGSSYRLQGEALASYREVAGEGIFRLSVGLEDAEDL
                     IADLARVLG"
     gene            4175..5083
                     /locus_tag="VIW27_05740"
     CDS             4175..5083
                     /locus_tag="VIW27_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5739206.1"
                     /translation="MAGKPPISDAAQQLLNQLQRNRISLPERDDLAHYRRDTRARFLP
                     WIERARKAFDGEIKDIEIAGVACRQLTPHDWQQQGGTCIQYAFGGGFFSGGVDEDLVI
                     AAPLAQLSHCRLIMVDYRLSPEHPYPQPQEDMRQVYPVLLDVFGAEHLAVCGESAGGN
                     QVLSLLQHARNNGQLMPACAALLSPWCDLADPLADDEGQDPTLDRSWARTAADWHADG
                     QALTDPGISPLYGDMTGLPPTLITSGSRDLLLAMSRRLADKMQSAGVDCELREHEGMW
                     HVFEYYPIPEAEQSLRDIAAYLEART"
     gene            complement(5062..5964)
                     /locus_tag="VIW27_05745"
     CDS             complement(5062..5964)
                     /locus_tag="VIW27_05745"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY5739207.1"
                     /translation="MNTDKPFKLPPEVIQRLSRRSDMRGLLQLVAHLGLLLLAGIGLQ
                     QAFGSAWVILALPVYAAVLIFLFAPLHETIHYTAFETRWLNQAVAAALGFLLLLPARY
                     FRVFHYAHHRHTQNPERDPELLERKPLTRSGYWLYLSGLPTWWQSLNGIWRHARGQLD
                     EPWIEARHRETIILEARSHLAGYGALLLLSLLFSSGFLWWYWVLPALIGQPLLRLYLL
                     PEHGGCDMSANMLENSRTTYTTPLLNFLAWNMPYHAEHHYLASVPFHALPALHAYTGQ
                     RVKYRADGYYRVNRELASQVRASR"
     gene            complement(5961..6494)
                     /gene="thpR"
                     /locus_tag="VIW27_05750"
     CDS             complement(5961..6494)
                     /gene="thpR"
                     /locus_tag="VIW27_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY5739208.1"
                     /translation="MTADDRQQRIFFALWPDAATRARLADIAAQIPLRRPARRVADYN
                     LHLTLHFIGTVDASVVDCMRRQARAVESAAFEIVIDDSGRFPGAGVGWLGCSEVVDGL
                     GRLHRRLGRELMRCDYLPEKRAYRPHVTVARKLGGALRPIAFAAVRWKVDNFVLLESR
                     PAQRGVRYHVVETYPLA"
     gene            complement(6522..6956)
                     /locus_tag="VIW27_05755"
     CDS             complement(6522..6956)
                     /locus_tag="VIW27_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5739209.1"
                     /translation="MKKSTSKPFMAAPDYGRSLAGFTVNLLSSDLARSLEFQRDVLRA
                     EILHADDDLLILRGYGSHWMVHADHTYDKHPLLADTSDQPRRGAGVELRLHGCDPDAA
                     AQRALQHGFRVLDGPRDQPDHGLRETHIIDGDGYVWVPDQSL"
     gene            complement(7014..8159)
                     /locus_tag="VIW27_05760"
     CDS             complement(7014..8159)
                     /locus_tag="VIW27_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739210.1"
                     /translation="MIVTRNLGIALLATVISFSTLYIPQPMLPLLAERFGVAPGEAGL
                     LMTVAMLPLAFAPVFYGYFLQAIPARLMLIVALGLLAVNQIAFYLVDAFWQLLLLRLL
                     QGLLLPAIFTALMTYCASTVPVQMVRRAMGYYIGATILGGFGGRLLGGLVASAFDWRT
                     AFVVAGLLQLVPLLLMLKADSDADINFARLDPHSISRVLARRDFRFMFVALATVFFVF
                     SGILNLAPFQLRQLQPEATPLLISLLYLGYLVGVPVSFYSHAISKRLADERGGLLLGL
                     ALHACGLLMLLFVDFQGMVATMFLLAAGFFLIHALLSGLANHLASEHRGVVNGLYVSV
                     YYLSGALGSWLPGYLYEAVAWHGMIVLFLGLLAVCAWSMSRLRVTGG"
     gene            8337..9233
                     /locus_tag="VIW27_05765"
     CDS             8337..9233
                     /locus_tag="VIW27_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739211.1"
                     /translation="MWIPLTILAAFFQNIRSVLQKHLQGKLSTLGAAYVRFLYAWPLA
                     LVYFCIVLFTERQGIPEFGPAFLGYALLGGICQILFTVFLLWLFSFHNFAVGTTISKL
                     ETVMVAIFGLLLLGDSLTPTVVLAIALSAVGLIVISVDQFGLGLRGMLRGLWRLPTLI
                     GLACAAWLGLSVVLFRAASLSLGQDSFLVAASFTLLIVLLLQTAIMGAMIAIREPSQL
                     KQVLVHWRPAALVGLSGGIASVGWFSAFTLQNATYVRALGQIELVFTFVATLIFFREK
                     VSLAEIVGIGLVTMAILIILLA"
     gene            complement(9883..>10885)
                     /locus_tag="VIW27_05770"
     CDS             complement(9883..>10885)
                     /locus_tag="VIW27_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739212.1"
                     /translation="FILDVEFTNQMIGRLFISGIGGCFLVFCFFKFGLHYKIASLGRF
                     TTAFYYAVFEYKKVQTEPSDMVYVDSVRQQAEVALAANFDPTLTRGHLAIPVTLYSGK
                     DPYLEELRAKIKEAEKLKLKSHQLDTQANILLDKTQEDMVEKNLVTGEKPRLLDRWFE
                     MVEGTEAIINLTTDELKEQGYGNPPDYEMISRIALDRNPHFSATFKFFDSEAIAGLLG
                     RAPFMIILVGIIGTFAGFYLALAQGGDIKAGASVAIVSSLVGLPTALLMEYINTLFPD
                     QERYQRAFGTYKVALELLFNHEQELSSIRQDRRKEDKLPGESYQPDERETSPYSGGH"
BASE COUNT         2207 a         3419 c         3264 g         1995 t
ORIGIN      
        1 agtctcggcc tgtataccac gcgcgcggac ctcgaagcgc tggtcgccgc ggtgcgtgat
       61 ctcgccggcc gccgcgacga aattctggcg ctttacgacc cgatcggctc gaacggctat
      121 cggcaccgcg aatttaagcc cgcggcgcag gacctgttcg atcccgagcg ctcgctggaa
      181 ctctacctgc aggcttccta gccgggcatg ccggcggctt cagcagccgg cggcagtcgc
      241 ggcccggcaa ccgcccaggg accgctttag gagccgccga gacgttcgcc acggcggccg
      301 cccgcaatac gcgggccctg gctggttacc ggcccatctg tatcgctgtg ccaacccgcc
      361 tgtagctcgc ccgcggcatc cccgggggcg ccccgcggcg cattcccccg attccgctgc
      421 gcaaatcccg atcagccctc gccaaccatg ccctgccccc cccctctgtg cggtgtcgtc
      481 gagatcacat ttcaggcatt ttttcaggct aatttccttc agcttttttc acagcgtcta
      541 tactcgaaaa aattttcgcc tttcgaataa ccagaattgc tgaattatgg aaacaccaat
      601 cgataattac ctcaaaatcc tcgcccacaa ggacggttcc gatctctacc tgagcaccgg
      661 cgcacccgcc tgcgcgaaat tcaacggcgt gctcaaaccc ttgtcgcaaa acgcctacaa
      721 ggcaggtgaa atcgaggcga tcgccaatga aatcatggac gaggagcagc gagaggaatt
      781 cgtccacgag ctggaaatga acctggcgat atcgatcccc ggggtcgggc gttttcggat
      841 caatattttc aagcaacgca acgaagtttc catcgtcgcg cgcaacatca aggcagagat
      901 tcccaagttc gatgacctgg gattaccggt ggtgctgaag gagatcatca ccgccaagcg
      961 cggtctggta ctgttcgtcg gcggtaccgg ttcgggtaaa tcgacctcgc tggcggcact
     1021 gatcgatcat cgcaactcga actcggcggg acatatcatc accatcgagg atccggtcga
     1081 atttgtccac aagcacaaga aaagtatcgt caatcaacgc gaagtgggag tggacacccg
     1141 cacttttcac aatgcgctga aaaataccct gcgccaggct cccgatgtga tcctgatcgg
     1201 tgaaatccgt gagcgcgcca ccatggagca cgccctggaa ttcgccgaaa ccggacacct
     1261 ggcgatatcg acactgcacg ccaacaatgc caaccaggcg ctggagcgta tcatcaatat
     1321 gtttcccgaa gagcgccgtc agcagctgtt gctggggctg gcacagaaca ttcgcgcaat
     1381 cgtttcccag cgcctggttc cgaccaccga caacaagcgc tgtgccgccg ttgaagtgtt
     1441 gctggggacc aagacgattc aggaactgat cctcaagagt cggctcaatg aaatcaagga
     1501 gatcatggaa aaatcagaag gccttggcat gcaaaccttc gatgccgccc tgttcaagtt
     1561 atacatgagc caggtcattt cactcgacga agcgctggcc aatgccgatt ccccgaacaa
     1621 cctgcgcttg cgcatcaagc tggcaaacga gtcaggcccg gaaggcgatg cctcggccgc
     1681 cgacggtagc gaggcggcgg ccgaatccgc tttcggcgag ttatccctgg aagaaatcga
     1741 agagccggaa gacgaagagc ccggggagga ttccgggatc gttaatatac tcgatcgcaa
     1801 ggcgcaataa acaccgcgga tcttttccgc tcgccgctac cagtgagccc ccagcaccat
     1861 tcgaaacgta ttcgccggcc tcgccgacag ccggtcggcg gccgctgcat gccacgggca
     1921 gccgggtagc cttcgatagc taatcactaa tcccggcccg aactcgcctg ctgatcgaaa
     1981 aacgacgccg atctcggcat ttgcggcaac agaatgtcgt agcccgtggc gcggacgcgg
     2041 ccagtcgcga caccgcgcac gacctcgccc tggtggcggc agggcaccgg gctgggcgcg
     2101 cacggaaccg cgtcctccgc cgaataaacg acgatgaaca gcgtgcgcgg ccgcggcgaa
     2161 cggttcgctg ccgatccgtg taacaggcgg gtatgcatca gacacagggt tccagccgtg
     2221 ccggtgcagc tgacggagcg agccaggcaa tcgcgggcca ggtcgtcgtc gatcgcaccg
     2281 gtgaattcgc catcgtgcca caggtcgtaa atcgcgccgc gatgggatcc gggcagaacc
     2341 tgcagcggac cgttttcctc gctgaggtcg tcgagcatca gcagtgccgt caccaggtcg
     2401 tcgttactat gcggcgtaaa tgcaaaatcc tggtgccact tgaccgcggt cgccgcaccc
     2461 ggcagcttgg aattgatctt gctgtgatga aacttgacgt tcggcccgat cagttcggcg
     2521 acataatcgg cgatgcggct gtcgcgcatc gcggcgaggt actcggcgga gatatcggtc
     2581 ggcgcattga cgcgcctcag cgcaggtcga tctttatcgt gcccgggttc gagatcgaag
     2641 cgcgcgcggc catcgagcgt cttgccccag ggacggcatt gttgccggct tgcctcgacc
     2701 cagtcgctga actggttgcg caaggcactc acctgatcgg cgtcgaccgc gttttccacc
     2761 agcaggtaac cttcgtcggc gaagcgctcg cattgccatg attgaaggaa cggcatcgtc
     2821 aaagtctcga aaatacctaa tatagcgaaa acggcgggta tcataaaccg cgtcgccatc
     2881 gacgccgaag gcggtaagcc gactcgatga cgattgacat cgagcaaaat cggccttact
     2941 aatgtgcatt gacaggcata gagcgaacga tgaccgatag cccgcacgac gacatgggac
     3001 tgcagacact ggcgatccac ggcggtgaat cgcccgaccc gagcaccggc gcttcgtcac
     3061 ccaacctggt aatgtcgagt acctacgtgg tcgacgagga agtcagtttc tcggccaaca
     3121 acctggcggc cgagacgccg ttcgtctaca cgcgctggga taatcccacc agtcgccagc
     3181 tgcagagcaa gctgtcgctg ctcgaaaacg ccgaggcttg cctggccttc gcgtcgggca
     3241 tggcggcgag ctgcgcggtc ctgctggcgc atctcgatcc tggcgaccat ctggtgatca
     3301 gcgataccaa ttacccgggc accgccgagt tcgtgcgcaa caccctgacg cgcctcggcg
     3361 tcgaggtcag cccggtcgac acctcgcaga acggcgcgat cgaggcggcg ctgcgcgaca
     3421 atacgcgcat gctgtggctg gagacgccat ccaacccgct gctgaagatc gccgatattc
     3481 gcgccgccgc cgaactcgcg cacgcgcgcg gcgcactgct ggtcgtcgat tcgactttcg
     3541 cgacgccgat cgcgacccgg ccgctcgaac tcggcgccga tctggtcgtg cattcgctga
     3601 ccaagtatat cggcgggcac ggcgatgcca tgggtggcgc ggtctgcggc aaccaggacc
     3661 tgctcggcgc actgcgcggc gaagcgctgg tgcactacgg cggcgtgatc agccctttca
     3721 atgcctggct gatcctgcgc ggcatggcga cgctgccgct gcgcatgcgc tgccaccagc
     3781 aaaacgcgct cgcggtagcg gacttcctcg aagcccacgc gctggtcgag cgggtctact
     3841 accccgggct cgaatcgcac ccgcaacacg cgctggcgag ttcgcagatg cgcaattttt
     3901 cgggcatgat cagctttcgc agcgcacagc ccaaacatgt cgccgcacgc atgatgcagg
     3961 agctgcgggt gattcactac gcggtctcgc tcggtcacca tcgcagcctg atctacctga
     4021 tgcagaccga cgacctggtc ggctcttcct atcgcttgca gggcgaggcc ctggcaagct
     4081 atcgtgaagt tgccggcgaa ggtatctttc gcctgtcggt tggcctcgag gatgccgagg
     4141 acctgatcgc agatctcgcc cgggtactcg gctgatggcc ggcaaaccgc cgatcagcga
     4201 cgcggctcag caactgctca accagctgca gcgcaaccgc atcagcttgc cggaacgcga
     4261 tgacctggca cattatcgtc gcgacacccg cgcccgcttc ctgccctgga tcgagcgcgc
     4321 gcggaaagct ttcgacggcg agatcaagga catcgaaatc gccggcgttg cctgccggca
     4381 gctgacaccg catgactggc agcagcaggg aggcacctgc atccagtacg ccttcggcgg
     4441 cggctttttc agtggtgggg tcgacgaaga cctggtgatc gccgcaccgc tcgcgcaact
     4501 gagccactgc cggctgatca tggtcgatta ccgcctgtct cccgagcacc cctacccgca
     4561 gccacaggag gacatgcgcc aggtctaccc ggtgctgctc gatgtcttcg gtgccgagca
     4621 cctcgccgtc tgcggcgagt cggcgggcgg caaccaggtt ctgtcactgt tacagcatgc
     4681 gcgcaacaac ggccagctga tgccggcctg cgccgcgttg ctgtccccgt ggtgcgacct
     4741 cgccgatccg ctcgccgacg acgagggcca ggatccgact ctcgaccgca gctgggcgcg
     4801 caccgcggcc gactggcacg ccgacggtca ggcgcttacc gatccgggaa tttcaccgct
     4861 ttacggagac atgacgggct tgccgccgac actgatcacc agcggcagcc gcgacctgct
     4921 gctggcgatg agccggcgac tcgccgacaa gatgcaaagc gccggcgtcg actgcgaact
     4981 gcgcgagcac gagggcatgt ggcacgtgtt cgagtattac ccgataccgg aagccgagca
     5041 gtcgctccgc gatatcgccg cctacctcga agcgcgcacc tgagacgcga gctcgcggtt
     5101 gacacggtaa tagccgtctg cccggtactt gactcgctgc ccggtatagg cgtgcagcgc
     5161 aggcaaagca tggaacggga ccgacgccag gtagtggtgc tcggcatggt agggcatgtt
     5221 ccaggcgaga aaattcagta gcggcgtggt gtaggtagtg cgtgaatttt ccagcatgtt
     5281 ggcactcata tcgcagccgc cgtgctcggg tagcagatac aggcgcagca acggctggcc
     5341 gatcagcgcc ggcaacaccc agtaccacca tagaaacccg ctcgaaaaca gcaggctgag
     5401 cagcaatagc gcaccatagc cagccaggtg cgaacgtgcc tcgaggatta tcgtttcgcg
     5461 atggcgcgct tcgatccagg gttcatcgag ttgcccgcga gcatggcgcc aaataccgtt
     5521 caggctttgc caccaggtcg gcagcccgct caggtataac cagtaacccg agcgagtgag
     5581 cggcttgcgc tccagcaact cggggtcgcg ctcggggttt tgcgtatgcc gatggtgggc
     5641 gtaatgaaac acgcgaaaat accgcgccgg cagcaacagc aaaaagccga gcgccgcggc
     5701 caccgcctgg ttcagccagc gggtctcgaa cgccgtgtag tgaatggttt cgtgcagcgg
     5761 tgcgaacagg aagatcagca ccgcggcata gaccggcagg gcgagaatta cccaggcgct
     5821 gccgaacgcc tgctgcagtc cgatgccagc cagcaacagc aatccgaggt gcgccacgag
     5881 ttgcaacagt ccgcgcatgt cggatcgccg cgaaaggcgc tgaatgactt ccggcggcag
     5941 cttgaaaggt ttatccgtat tcatgccagc ggataggtct cgacgacgtg atagcgaacg
     6001 ccccgctgcg cgggccgcga ttccagcagg acgaaattat ccaccttcca gcgcaccgca
     6061 gcaaacgcga tcggccgcag cgcgccgccc agcttgcgag ccaccgtgac atgcggccgg
     6121 taagcgcgtt tctcgggcag gtaatcgcaa cgcatcaact cgcggcccag ccgccggtgc
     6181 aggcgaccaa ggccgtcgac aacctcgctg cagcccagcc agccgacgcc ggcaccgggg
     6241 aaccgcccgc tgtcatcgat cacgatttca aacgcagccg actcgaccgc ccgcgcctgc
     6301 cggcgcatgc aatcgaccac gctagcgtcg acggtgccga tgaaatgcag cgtcaggtgc
     6361 aggttgtaat ccgctacgcg ccgtgcggga cgccgcagcg gaatttgcgc ggcgatgtcg
     6421 gcgagacgcg cgcgtgtcgc ggcgtcgggc cacagcgcga aaaaaatgcg ttgctggcga
     6481 tcgtcggcag tcaatgtcgg tggctagcgc cggcgaatgc atcaaagcga ctggtcgggc
     6541 acccagacat agccgtcgcc atcgatgata tgcgtttccc gcaagccatg gtcgggctgg
     6601 tcgcgcggcc cgtcgagcac gcgaaagcca tgctgcagtg cgcgctgcgc cgcggcgtcg
     6661 ggatcgcaac cgtgcaggcg cagttcgaca ccggcaccgc ggcgcggctg gtcactggtg
     6721 tcggcgagca acggatgctt gtcataagtg tgatcggcat gcaccatcca gtgactgccg
     6781 tagccgcgca ggatcagcag atcgtcgtcg gcgtgcagta tctcggcgcg caatacgtcg
     6841 cgttgaaatt ccagcgaacg cgcgagatcg gagctcaaca ggttgacggt aaaacccgcc
     6901 agcgaacgcc cgtaatcggg cgctgccatg aacggtttgc tggtgctctt tttcatcgtc
     6961 gtccaagcct atcatcaaag cgttcgccga caaacggcta acggcacgat cgctcagccg
     7021 ccggttacgc gcaggcggct catgctccac gcgcagaccg cgagcaggcc caggaacagc
     7081 acgatcatgc catgccaggc gacggcctcg taaagataac cgggcagcca cgaccccagc
     7141 gcgccgctga ggtaatacac cgacacgtac aggccgttga ccacgccgcg atgctcgctg
     7201 gcgaggtggt tagcgagccc cgacaacaac gcgtgtatca ggaaaaagcc ggcggcgagc
     7261 aggaacatcg tcgccaccat accctgaaag tcgacgaaca gcagcatcag cagaccgcag
     7321 gcatgcagcg ccagtcccag caacaggccg ccgcgctcgt cggccaggcg cttgctgatc
     7381 gcgtgactgt aaaaggatac cggcacgccg accaggtagc cgaggtacag caacgagatc
     7441 agcagcggcg tcgcctccgg ttgcagttgt cgcagctgaa acggtgccag gttcaggatt
     7501 ccggagaaga cgaagaacac cgtcgccagc gcgacgaaca tgaaacggaa gtcgcgcctt
     7561 gccagcaccc gagaaatact gtgcggatcg aggcgcgcga agttgatgtc ggcatccgaa
     7621 tcggccttca gcatcagcag cagcggcacc agttgcagca gcccggcgac cacgaacgcg
     7681 gtgcgccagt caaaggcact ggcgaccagg ccacccagca ggcggccgcc gaaaccgccg
     7741 aggatggtcg cgccgatgta ataacccatc gcacggcgca ccatctgcac cggcacggtg
     7801 gaggcgcagt aagtcatcag cgcggtgaag atcgccggca gcaacagccc ctgcagcagg
     7861 cgcagcagga gcaattgcca gaaggcgtcg accaggtaaa aggcgatctg gttcacggcc
     7921 agcaggccga gcgcgacgat cagcatcagg cgcgcgggaa tggcttgcag aaaataaccg
     7981 tagaacaccg gcgcgaacgc cagcggcagc atcgccacgg tcatcagcaa gccggcttcg
     8041 cccggtgcaa cgccgaagcg ttcggcgagc agcggcaaca tcggctgcgg aatgtacagc
     8101 gtgctgaacg agataaccgt cgccagcagc gcaatgccga gattgcgggt cacgatcatg
     8161 aaggcactat ccctggcacg gcgaatatcg gcgccttcgg caggtagttt atcatcgcgt
     8221 tattcgtttc tcccgggtga tactcacccc agccgaatcc ccggctggcg agccgcatca
     8281 taacaattcc atgcgtcgcg tttcagccga tttgctatac actgcggcgc atggaattgt
     8341 ggattcccct gaccatcctg gcggcgtttt ttcagaacat ccgctcggtc ctgcaaaaac
     8401 acctgcaagg caagctgtcg accctcggcg cggcctacgt gcgtttcctt tatgcctggc
     8461 cgctggcact ggtctatttt tgcatagtcc tgttcaccga gcggcagggg attccggagt
     8521 ttggcccggc tttcctcggc tacgcgttac tgggcggtat ctgccagatc ctgttcaccg
     8581 tgttcctgct gtggttgttt tcgttccaca atttcgccgt tggtacaacg atctccaagc
     8641 tggaaacggt gatggtcgct atcttcggcc tgctgttgct cggcgactcg ctgacaccga
     8701 ccgtggtact cgccatcgcg ctgagcgcgg tcggtctgat cgtgatcagc gtcgaccagt
     8761 tcggtctcgg gctgcgcggc atgctgcgcg gcctgtggcg cctgccgact ttgattggcc
     8821 tcgcctgcgc cgcctggctg ggattgagcg tggtgctgtt ccgcgccgcc tcgctgtcgc
     8881 tcggtcagga tagttttctt gtcgcagctt ccttcacgct gctgatcgtg ctgctcctgc
     8941 aaacggcgat aatgggcgcg atgattgcca tccgcgagcc gtcacaactg aagcaggtgc
     9001 tggtgcactg gcgaccggcg gcactggttg gattgtccgg cggcatcgcc tcggtgggtt
     9061 ggttcagcgc cttcacgctg caaaacgcga cctacgtgcg cgcgctcggg cagatcgaac
     9121 tggttttcac cttcgtcgcc acgctgatct tttttcgcga aaaggtcagt ctcgccgaaa
     9181 tcgtcggcat cggcctggtc accatggcga tcctgatcat cctgctggcc tgatggttac
     9241 acagccaggc tctctgtttt gcgccagcag agatttacct gaaaaggcat ttgatatcgc
     9301 tcagcatcgc cagaatccta ctgccaaaac ctaattacgc agaaaaacca atcagcacca
     9361 aaaccgatat gaccgaaaca gatcggctga ccctgccccg aacaggtggg ttatgcttcc
     9421 tatactttgg ttgatcaaag tcttgtcgaa tgacacttga tcccgggcag caatatgcat
     9481 ccaggcccga ttaaaattgc gcatgaagag acacctcatc cgtcaggaag ccattcatcg
     9541 gcgacgattc aatcgtgagg tgccgcagtt gaacacggca taggtgttcc gcacaaaacc
     9601 ttcccgctcg ggccgttatc gcaacgggcg cggccatcta ttgctcgcat ccaggattcg
     9661 accgatatat agcctttgaa ccgggtatcg aggagtttcg ccacctaatt aatcgccgat
     9721 agctacattg aatccggaca acgtcgcatt tgactgtgcc attcataaag gtgggtgtcc
     9781 ttttgatact tttgataggt gttttgatac tctggggccg cggccaaaaa aaagggccgg
     9841 tgactggcct gtcaccggcc cagctgtgct cggccctcgg ggttaatgtc cgcccgagta
     9901 cggcgaagtc tcacgctcgt ccggctggta tgactccccg ggcagcttgt cttccttgcg
     9961 gcggtcctgg cggatgctgg agagttcctg ttcgtggttg aatagcagtt ccagcgcaac
    10021 cttgtaggtg ccgaacgctc gctgatagcg ttcctggtcc gggaacagcg tgttgatata
    10081 ttccatcagc agcgccgtcg gcaggccgac cagcgagctc acgatggcga ccgacgcgcc
    10141 ggccttgatg tccccgccct gcgccagcgc gaggtaaaag ccggcgaagg taccgatgat
    10201 gccgaccagg atgatcatga acggcgcgcg gccgagcagc cccgcgatcg cttccgaatc
    10261 gaagaatttg aaggtcgcac tgaagtgcgg attgcgatcg agcgcgatgc ggctgatcat
    10321 ttcgtaatcc ggcgggttgc cgtagccctg ctccttcagc tcgtcggtgg tcagattgat
    10381 gatcgcttcg gtgccttcga ccatttcgaa ccagcgatca agcagcctcg gcttttcgcc
    10441 ggtgaccagg tttttctcga ccatgtcttc ctgggttttg tcgagcagaa tattcgcctg
    10501 cgtatcgagc tggtgcgact tgagtttgag tttttccgct tccttgatct tggcgcgcag
    10561 ctcctcgagg tagggatcct tgcccgagta aagcgtgacc ggtatggcca ggtgaccgcg
    10621 ggttaacgtc ggatcgaagt tggctgcgag cgcgacctcg gcctgttgcc tgacgctgtc
    10681 gacgtagacc atgtcggaag gttccgtctg cactttcttg tactcgaata ccgcgtaata
    10741 aaacgcggtc gtgaaacggc cgagagacgc gatcttgtaa tgcaggccga acttgaaaaa
    10801 acagaacacc aggaaacatc ccccgatacc gctgataaac agcctgccaa tcatttgatt
    10861 ggtgaactcc acgtccagaa tgaac
//
LOCUS       DATCZV010000259         4439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_550910, whole genome shotgun sequence.
ACCESSION   DATCZV010000259 DATCZV010000000
VERSION     DATCZV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550910"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VIW27_05775"
     CDS             <1..679
                     /locus_tag="VIW27_05775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739213.1"
                     /translation="TEFSLDWYVKAAQNEGIQHAAWISLEVAFIAMVGSTILATMAAL
                     VTTRTPPYRGLNLAVAVINQPLMVPEVVTAVATLIFFAIIKKITGVTGIGYLIAAHTA
                     FCVPFAYMPIRARLEDMDLSLEQAAADLYAPPFQVFRRVTLPLMMPGILAGAMLAFIV
                     SLDDVIITLFVAGPGETTLPLYILGQIRRGVTPEINAVSTVFLGISVILVTLIFLFGT
                     DKNKGKQ"
     gene            737..1768
                     /locus_tag="VIW27_05780"
     CDS             737..1768
                     /locus_tag="VIW27_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009178648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5739214.1"
                     /translation="MKHKLLVSITALWLTLAMGSAQAAGELNIYNWGNYTNPDMIKKF
                     EQEYDVKVNLDGYDSNETMLAKVEQGGSGYDIVVPGDYMISIMIKKGLLARVEPNKMS
                     NFKNVDPKWVDVYWDEGRNYSIPYQWGTTNFTVDATVYKGDINTLALMFDPPDELKGR
                     INMLKDMNDVINAGLRYLDYPRCNSDKAQLKELNSMLVKAKANWRTMDYSVIEKLTSK
                     DVDLSQSWNGAAKRARKDRPELVYAYPKEGFTGWMDNVAVLKDAPNMENAKLFMNFMM
                     TPEAAAMTSNFANYANGIIGSESMMNADLTAAPEIVMPAGSPAPEFVPPCPQEVTDLY
                     SKIWTNLNK"
     gene            1832..3046
                     /locus_tag="VIW27_05785"
     CDS             1832..3046
                     /locus_tag="VIW27_05785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5739215.1"
                     /translation="MAKTRLKPRTAQELGIMRGFPPPPEKRPDLTNWDLAPFNRWSFM
                     NIRNLFPTVDVKADRVGARELPAAHQDLSALEFFDHAGRRVTLAEYLDGSYTDGFLVL
                     HRGRIICENYFNSMQPDTPHLSQSVAKSLIGALAGILHERGLVDEDAPLVSYVPELAR
                     CGYKDAKLSHALNMTSGIRFVEDYNMPDSDMTRIDIASGWRPPRGQEAQPTIRDIILT
                     LPKVRRHGKVFAYKSVETDVVAWVLERAANRSLADMVSELIWKKIGAEHDAFFTVDRA
                     ATALADGGFNATLRDYARFGLMMQNSGMVGDTEVVPRRWVDACASGDRSVYGPPAADI
                     CPDGAYSNFWWDNNIESGDFMARGVFGQMIYVNREASVTIVKLSTWPDYVITDFTRDS
                     LAAFAAILEYLR"
     gene            complement(3558..3767)
                     /locus_tag="VIW27_05790"
     CDS             complement(3558..3767)
                     /locus_tag="VIW27_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739216.1"
                     /translation="MEENRLAVNGALGKSRNPHAGLVCGIHARNDARRGVWKAAASGV
                     CGGVLCFERDVNSAQQQLLLPIDVN"
     gene            3891..>4439
                     /locus_tag="VIW27_05795"
     CDS             3891..>4439
                     /locus_tag="VIW27_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5739217.1"
                     /translation="MTATLTIGQMLSAQARLQPERLAARDLDRGLTYREWNARACRLA
                     NALLGLGLAHGDRVAVLAYNRLEWAEIYTAVAKAGLVAVPINFRLTAPEVQFIVEDCA
                     VAAILVEDRLAGVIDEIRDQLSLGEDRYVVIGDSARAPGYRAYETLLGAAAEQEPEQT
                     VAPQDTWCLMYTSGTTGRPKGAI"
BASE COUNT          896 a         1470 c         1332 g          741 t
ORIGIN      
        1 gaccgagttc agtctcgact ggtacgtcaa ggccgcgcaa aacgaaggca ttcagcacgc
       61 ggcatggatt tcgctcgagg tcgccttcat cgcgatggtc gggtcgacga ttctcgccac
      121 catggccgcg ctggtgacca cgcgcacccc gccctaccgc gggctcaacc tcgcggtggc
      181 ggtgatcaac cagccgctga tggtgcccga ggtcgtcacc gcggtggcga cgctgatctt
      241 ttttgcgatc atcaagaaga tcaccggggt gaccggtatc ggctacctga tcgcggcgca
      301 caccgcgttt tgcgtgccgt tcgcctacat gccgattcgc gcgcgcctgg aagacatgga
      361 tctcagcctc gagcaggccg ccgccgatct ctacgcgccg ccgttccagg tatttcgccg
      421 cgtcacgctg ccgctgatga tgcccggcat tctcgccggc gcgatgctcg cgttcatcgt
      481 ttcgctggac gacgtcatta ttacgctgtt cgtcgccggt cccggcgaaa ccacgttgcc
      541 gctgtacatc ctggggcaga ttcggcgcgg ggtcacaccc gaaatcaacg cggtatcgac
      601 cgtgtttctc ggcatttcgg tgatcctggt gaccctgatt ttcttattcg gaaccgacaa
      661 aaacaaaggg aagcagtaac tcacgtccgt gagcaaatcg agcagaaaca atgcaacaac
      721 taactgagga aaagctatga aacacaaact actggtatcc atcacggcgc tgtggctgac
      781 cctggccatg ggcagcgcgc aggccgccgg cgagctgaac atctataact ggggtaacta
      841 caccaacccg gacatgatca agaagttcga acaggaatac gacgtcaagg tcaacctcga
      901 cggttacgat tccaacgaaa ccatgctggc caaggtcgag cagggcggct cgggttacga
      961 catcgtcgtg cccggcgatt acatgatctc gatcatgatc aagaagggcc tgctcgcgcg
     1021 cgtcgagccg aacaagatgt ccaacttcaa gaacgtggac ccgaaatggg tggacgttta
     1081 ctgggacgaa ggccgcaact actcgatacc gtaccagtgg ggcacgacca acttcaccgt
     1141 cgacgccacc gtgtacaagg gcgacatcaa cacgctggcg ctgatgttcg atccgccgga
     1201 cgagctcaag gggcgcatca acatgctcaa ggacatgaac gacgtcatca acgccggcct
     1261 gcgctacctc gactacccgc gctgcaactc ggacaaggcg cagctgaagg aactcaacag
     1321 catgctggtc aaggccaagg ccaactggcg cacgatggat tactcggtga tcgaaaagct
     1381 gacctcgaag gacgtcgacc tgtcgcaaag ctggaatggc gcggccaagc gcgcgcgcaa
     1441 ggatcggccg gagctggttt acgcctaccc gaaggagggc ttcaccggct ggatggataa
     1501 cgtcgccgtg ctcaaggatg cgcccaacat ggaaaacgcg aagctgttca tgaacttcat
     1561 gatgacgccg gaagcggcgg cgatgacgtc gaacttcgcc aactacgcca acggcatcat
     1621 cggcagcgag tcgatgatga acgcggacct gaccgcggct cccgaaatcg tcatgccggc
     1681 cggctccccg gcacccgagt tcgtgccgcc gtgcccgcag gaagtcaccg atctgtactc
     1741 caagatctgg accaacctga acaagtaaga cctcggtact gggcgggagc cccggctccc
     1801 gccccgtttt ccggggctac ccgaaacgac tatggccaag accaggttga aaccgagaac
     1861 cgcgcaagag ctgggcatca tgcgcggctt cccgccgccg ccggaaaaac ggccggacct
     1921 gaccaactgg gacctcgcgc cgttcaaccg ctggtcgttc atgaatatcc gcaacctgtt
     1981 cccgacggta gacgtcaagg ccgaccgtgt cggggcccgc gaactgccgg ccgcgcacca
     2041 ggacctgtcg gcgctcgaat tcttcgacca cgccggcagg cgcgtcacgc tggcggaata
     2101 tctcgacggc agctacaccg acggtttcct cgtgctgcac cgcggccgca tcatctgtga
     2161 aaactatttc aacagcatgc agccggacac gccgcacctg tcgcagtcgg tggcgaaatc
     2221 gctgatcggc gcgctggccg gaatcctgca cgaacgcggc ctggtcgacg aggatgcgcc
     2281 gctggtcagt tacgtgccgg aactggcgcg ctgcggttac aaggatgcga agctgtcgca
     2341 cgcgctgaac atgaccagcg gcatacgctt cgtcgaggat tacaacatgc ccgattcgga
     2401 catgacccgc atcgatatcg cctccggctg gcggccgccg cgcgggcagg aggcgcagcc
     2461 gacgatccgc gatatcatcc tgaccctgcc gaaggtgcgc agacacggca aggtgttcgc
     2521 ctacaagtcg gtggaaaccg acgtcgtcgc ctgggtactc gaacgcgccg ccaaccggtc
     2581 gctggccgac atggtcagcg aattgatctg gaaaaaaatc ggcgccgagc acgacgcctt
     2641 ttttaccgtc gaccgcgccg ccaccgcgct cgccgacggc ggcttcaacg ccaccctgcg
     2701 cgactacgcg cgcttcggcc tgatgatgca gaacagcggc atggtcggcg ataccgaggt
     2761 ggtgccgcgg cgctgggtcg atgcctgcgc cagcggcgac cgcagcgtct acggcccgcc
     2821 ggccgcggac atctgccccg atggggctta cagcaacttc tggtgggaca ataacatcga
     2881 aagcggcgac ttcatggccc gcggcgtgtt cgggcaaatg atctacgtca accgcgaggc
     2941 cagcgtcacc atcgtcaagc tctcgacctg gcccgattac gtgattacgg atttcacccg
     3001 cgacagcctg gccgcgtttg cggccatcct cgaatacctg cgctgaatcg gcatcgatcc
     3061 agcagtgaat acacggtcag gcctggcatc taccatccgc acttactgca gccgcggaat
     3121 ccagggcctg ccccccctgc caatctggct cagcgaaaaa atggcagtca gagagtcatc
     3181 tccaacaata ttagtaacaa ctgctctttg acacttacca ttccgacgag ccagacgagc
     3241 cgcccgttgt cgagatcgct gcggatcatc gcgccgcgag cgccacgcac gaagcaggct
     3301 tcgccgggtg cggtcccggg cagcgcgcca tcgcgccatc gcgccagtcg ttttagacga
     3361 aattcgaggc cgccccggcg cgggtcggcc catagccgcg catcgttggg ctcgaatacg
     3421 acccactgcg tatcgcgctc gatcgccgac tcgagatagt cgaaatagcg acgaaggttt
     3481 atctacttcc actgcggatc gaaggcagcg aggcgcgcgt cccggaccag gcagccgcgg
     3541 ccgggccgat ggcacagtca attcacgtcg atcggcagca gcaattgctg ctgcgcggag
     3601 ttgacgtcgc gctcgaaaca cagcacgcca ccgcagaccc cgctggccgc agccttccat
     3661 acgccgcgcc gggcatcgtt acgcgcatgg atgccgcaca ccaagcccgc atggggattg
     3721 cgggacttac ccaacgcccc gttgacagca agccggtttt cctccatttt gcagcctgga
     3781 gtcagtcatt tcggtaaccc gatgcaagcg atacgcccgt cctcgaagcg ttgcccgcgg
     3841 acctgtgcgg ctatcccgag gttcggtgcc accgcgcggg gctcgagtcg atgaccgcga
     3901 ctctcaccat cggccagatg ctgagcgccc aggcccggct gcagcccgag cgactcgcgg
     3961 cgcgcgacct cgatcgcggc ctgacctacc gcgaatggaa cgcccgcgcc tgccggcttg
     4021 ccaacgccct gctcggtctc ggcctcgccc acggtgaccg cgtcgcggtg ctggcctaca
     4081 accgcctgga gtgggccgag atctataccg cggtggccaa ggcggggctc gtcgcggtgc
     4141 cgatcaattt tcgcctgacc gcacccgagg tgcagttcat cgtcgaggat tgcgcagtcg
     4201 cggcgatcct ggtcgaagac aggctcgccg gcgtgatcga cgaaatccgt gaccagctat
     4261 cgctcggcga agaccgctat gtggtgatcg gggacagcgc cagggcgccc ggctaccgcg
     4321 cctacgaaac gctgctcggc gcagccgccg agcaggaacc cgaacagacc gtcgcgccgc
     4381 aggacacctg gtgcctgatg tacacctcgg gcaccaccgg ccgccccaag ggcgcgatc
//
LOCUS       DATCZV010000260         4121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_391243, whole genome shotgun sequence.
ACCESSION   DATCZV010000260 DATCZV010000000
VERSION     DATCZV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391243"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..337
                     /locus_tag="VIW27_05800"
     CDS             167..337
                     /locus_tag="VIW27_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739218.1"
                     /translation="MRGGVERRSANLERAWTTVVDIELGNFRLGRSHYLARALRADYL
                     HVEALDAPPPRG"
     gene            complement(354..869)
                     /locus_tag="VIW27_05805"
     CDS             complement(354..869)
                     /locus_tag="VIW27_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739219.1"
                     /translation="MQISRILSWQPLILACSLLAAALSLPNATSAQSDGEKPKMVDFW
                     QPGDRGQRMRIRGRVTSLDGTPVPHVRIFFRHADSEGIDFSYHQGVVTANDKGVYQFG
                     SVIPGNSHRLSHVHVYVNHDDYQYLDTEFYFKDDPKTADDDPRAIFLEAGTVGGETMM
                     FGRFDITLIPH"
     gene            complement(1000..2055)
                     /locus_tag="VIW27_05810"
     CDS             complement(1000..2055)
                     /locus_tag="VIW27_05810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5739220.1"
                     /translation="MRVSILLLLGWMTPALATDHAVGLLYHHVDTGTPASTSVTPKTF
                     ETHLDYLADNGFAVRPLGEVLATLHRGGSLPPNTVSITFDDAYTSVFEEAYPRLRARG
                     WSATLFVNSEAIDRGFRNYMSWAQVRELAAAGFEIGNHSHTHAHLVRRLANESQADWR
                     ARVTADIELAQRRIAAETGSEPRLFAYPYGEYSPALQQVVDRLGFTGVGQHSGALGAS
                     SDFLAVPRFPMSTGYADLQRFATAVNARPLPVITARAGGERAIDGRLDSLELEVAPGA
                     YRAGQLACYSAAGKRLDLKAGDGQPLRLRVTLQEAQPPGRNKVNCTAPAADERGAYFW
                     YSYLWLVKNADGSWYRE"
     gene            complement(2058..2849)
                     /locus_tag="VIW27_05815"
     CDS             complement(2058..2849)
                     /locus_tag="VIW27_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEY5739221.1"
                     /translation="MRVPRIKLPRIKHRLVLAVVTAAAIAAAALAMLVSPAPSNAELP
                     DMTRFAAGAERKAAFFGFLEPIVIAKNARILRDRASLREMADDLDAGRSLSSRQLDRL
                     ESLAEKYALVDDDADPAQRVRQLLLRADAVPLPLVMVQAAKESGWGTSRFAREANNLF
                     GQWCFEPGCGIVPKRRAAGARHEVRDFDSVEDAVTAYLHNINTGRAYQRLREIRADLR
                     RAGEKPSAIALSDGLLFYSERREEYVNEVKRMILQYHKFQQPRET"
     gene            3033..3308
                     /locus_tag="VIW27_05820"
     CDS             3033..3308
                     /locus_tag="VIW27_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739222.1"
                     /translation="MTGKQSADDLSEAAQVAWQAFVDMQRSKSAHFTLLQAITSARES
                     GDVPSPADNLELEKRLAEHDRNVLAFTTAMAAVTDDDEIQALVRLMS"
     gene            3574..3861
                     /locus_tag="VIW27_05825"
     CDS             3574..3861
                     /locus_tag="VIW27_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739223.1"
                     /translation="MTPAVLLGLYYLYALFNGWSVEVVELRPSRCELVEHFDSSYFKY
                     RNEGILELKSKTASLGADTLYAPFPRDVPYGLSATDDRQVFVPARAYRCDS"
     gene            3924..>4121
                     /locus_tag="VIW27_05830"
     CDS             3924..>4121
                     /locus_tag="VIW27_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739224.1"
                     /translation="MSGYLEPQRVSVRDIPHWARESWQLLWRRPLGFFAASLAYHGLA
                     LSSQSIPWIALLLSVLLCYMLL"
BASE COUNT          677 a         1287 c         1388 g          769 t
ORIGIN      
        1 gatcgacggc gtgctgatca gtggctcgcg cggtagcgcc caagtcgacg ctggtcagga
       61 gcatcgaggc actggtcgcg gatcgtcagc tggtaaccct gccgctcggc gccagcgagt
      121 aaatgttggc tggaacgctg ccactgcagc aggcactgac ggccgggtgc gcggcggcgt
      181 cgaacggcgt agcgccaacc tcgaacgtgc ctggaccacc gtggtcgata tcgaactcgg
      241 caatttccgt ctcggccgca gccactacct ggcgcgcgca ctgcgcgccg attacctgca
      301 cgtcgaagcg ctcgacgcgc cgccgccgcg cggctgaggg tgggtgccgc ggatcagtgc
      361 ggaatcagcg tgatgtcgaa gcggccgaac atcatggttt cgccgccgac ggtgccggct
      421 tcgaggaaaa tcgcccgcgg atcgtcgtcg gcggttttcg ggtcgtcctt gaaataaaac
      481 tcggtatcca ggtactggta atcgtcgtgg ttgacataga cgtgtacgtg gctcagccga
      541 tggctgttcc cgggaatcac cgagccgaac tggtagacgc ccttgtcgtt ggcggtcacc
      601 accccctggt ggtagctgaa gtcgataccc tcgctgtcgg cgtggcggaa aaagatcctg
      661 acgtgcggca ccggggtgcc gtcgaggctg gtcacacggc cgcgaatgcg catgcgctgg
      721 ccgcggtcgc cgggttgcca gaagtcgacc atcttcggtt tttcgccgtc ggactgcgcg
      781 ctggtcgcgt tcggcagcga tagcgcggcc gccagcaggc tacaggccag gatcagcggt
      841 tgccagctta aaatacggga aatctgcatg ttctgccctc caggtgtccg cacgccgagc
      901 attatacccg cacgcgaacc cactgcggaa tgccgccgtg cgacagcggc gggaacaccg
      961 cgcgccgcgc cgggtggtgg tgcccgcggt cggcgatggc tattcgcggt accagctgcc
     1021 atcggcgttt ttcaccagcc acaggtacga ataccagaaa taggcgccgc gctcgtcggc
     1081 cgccggtgcg gtgcaattga ccttgttgcg ccccggcggc tgtgcctcct gcagcgtcac
     1141 gcgcagccgc agcggctgcc cgtcgccggc cttgaggtcc agccgcttgc cagcggcgct
     1201 gtagcaggcg agttggccgg cccggtaggc gccgggcgcg acctccagct cgaggctgtc
     1261 gagccgtccg tcgatggcgc gctcgccgcc ggcccgcgcg gtgatgaccg gcagcggacg
     1321 tgcgttgacg gcggttgcga agcgctgcag gtcggcgtag ccggtcgaca tcggaaagcg
     1381 cggcacggcg aggaaatcgg agctcgcgcc gagcgctccg gagtgctgcc cgacaccggt
     1441 gaaaccgagc cggtcgacga cttgctgcaa cgccggtgaa tactcgccgt acggataggc
     1501 gaacagccgg ggctcgctgc cggtctcggc cgcgatgcgc cgctgcgcga gctcgatatc
     1561 ggcggttacc cgggcgcgcc agtcggcctg gctctcgttg gcgaggcggc gaaccaggtg
     1621 cgcgtgcgta tggctgtggt tgccgatctc gaaacccgct gcggccagct cgcgcacctg
     1681 tgcccagctc atatagttgc gaaaaccgcg gtcgatcgct tcgctgttga cgaacagggt
     1741 cgcgctccag ccgcgtgcac gcaggcgcgg gtaagcttct tcgaacaccg aagtgtaggc
     1801 gtcgtcgaaa gtgatcgaga ccgtgttcgg cggcaacgag ccgccgcggt gcagggttgc
     1861 gagcacctcg cccagcgggc ggaccgcgaa gccgttgtcg gcgaggtaat cgaggtgggt
     1921 ctcaaaggtt ttcggagtca cgctggtcga cgccggcgtg cctgtatcga cgtgatggta
     1981 gagcagaccg accgcgtggt cggtggccag cgcgggcgtc atccagccca gcaacagcag
     2041 gatcgagacg cgcaccttca ggtttctcgc ggttgctgga acttgtggta ttgcagaatc
     2101 attcgtttga cctcgttgac gtattcctcg cgccgctccg agtagaacag caggccgtcg
     2161 gagagcgcga tggccgacgg tttttcgcca gcgcgccgca ggtcggcgcg gatctcgcgc
     2221 agccgctggt aggcgcgccc ggtattgatg ttgtgcagat aggcggtgac cgcgtcttcg
     2281 accgaatcga agtcgcgcac ttcgtgtctg gcgcccgcgg ctcgtcgttt cggcacgatg
     2341 ccgcagcctg gctcgaagca ccactggccg aacaggttgt tggcctcgcg cgcgaaacga
     2401 gacgtgcccc agccggattc tttggccgcc tgcaccatca ccagcggcag cggcacggca
     2461 tcggcgcgca gcaacaattg ccgaacccgt tgcgcgggat cggcgtcgtc gtcgacgagc
     2521 gcatattttt ccgccagcga ttcgagacga tcgagttgcc gcgacgacaa gctgcgcccg
     2581 gcgtcgagat cgtctgccat ctcccgcagg ctggcgcgat cgcgcaggat ccgcgcattt
     2641 ttcgcgatca cgatcggttc gagaaaaccg aagaacgcgg ccttgcgctc ggcgccggcc
     2701 gcgaaccggg tcatgtccgg caactcggca ttcgatggcg ccggcgaaac cagcatggca
     2761 agcgccgcgg cggcgatagc ggctgcagtc accaccgcca gcacgaggcg gtgcttgatt
     2821 cggggcagtt tgatcctggg tactcgcatc gggattcggt cgtcttccgg cgaattcgcg
     2881 ccggccagcc cagattttat tgaagtccgg cgcaaaagta acgatcacgg gccccgccca
     2941 ggtaagcctg gcttcgccgg tggtggcgaa accggggtgc gcgatcggtt atactggtgt
     3001 ttcgactttt ctggcaatct ggtgcattta cgatgacagg caagcaatct gccgacgatt
     3061 tgagcgaggc cgcgcaggta gcctggcaag cctttgtcga tatgcagcgc agcaagtccg
     3121 cgcatttcac gttgctgcag gcgatcacca gcgctcgcga gtcgggcgat gtaccgagtc
     3181 ccgccgataa cctggagctc gaaaagcgac tcgccgagca cgatcgcaac gtgctggcgt
     3241 tcaccaccgc gatggccgcg gtcactgacg atgacgaaat tcaggcgctg gttcgattga
     3301 tgtcgtgatc ggcgggcgtt gccggcgagt gcgggcggtg gttggcgtcg agttgttcga
     3361 aacgcccgtt gcgcggtgtc ccgaggcgcg cgcgacggta ttcgcggtgc cgagtgcggg
     3421 cggtagcccg cgacccgtcc gggtgacgag gtcggcggca ggagatggca aaaaccgggg
     3481 tctgggctac attcatggtt tatggagcag aaacttttcg ccacagccag cgatttcgag
     3541 caaccacgtc gggttcgagc caactatttg tgggtgactc cggccgtatt gctcggactc
     3601 tattacctct acgcgctgtt caacggctgg agcgtcgagg tggtcgaatt gcggccgtca
     3661 cgttgcgaac tggtggagca ttttgattcg tcctacttca agtatcgcaa cgagggcatt
     3721 ctcgaactca agagcaagac cgctagtctc ggtgccgata cgctctatgc cccgtttccg
     3781 cgcgatgttc cctacggtct gtcggccacc gacgatcgac aggtcttcgt gcccgcccgg
     3841 gcttaccgct gcgattcctg atcgcaggtc cactcgtcgc ctgaccgcat tgccggcgat
     3901 agtcgacgca ctcacacggt gccatgtccg gctaccttga accgcaacgg gtaagcgtcc
     3961 gcgatattcc gcactgggcg cgggaatcct ggcaattact gtggcgccgg ccgctcggtt
     4021 ttttcgccgc ctcgctggct taccacgggc tcgcgctgag cagccagtcg atcccatgga
     4081 tcgccctgtt gttgtcggtg ctgctttgct acatgctgct g
//
LOCUS       DATCZV010000261         2726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_126479, whole genome shotgun sequence.
ACCESSION   DATCZV010000261 DATCZV010000000
VERSION     DATCZV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126479"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VIW27_05835"
     CDS             complement(<1..829)
                     /locus_tag="VIW27_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5739225.1"
                     /translation="MNNTETYNYKVVRQFSIMTIVWGLVGMAVGVLIAAQLAWPVLNF
                     DLPWLTFGRLRPLHTNAVIFAFGGCALFATSYYVVQHTCHARLAFPALAAFTFWGWQL
                     VILLAAITLPLGITTSKEYAELEWPIDILITLVWVSYALVFFGTILKRKIRHIYVANW
                     FFGAFIVTVAVLHVFNSAALPVSFWKSYSAYAGVQDAMVQWWYGHNAVGFFLTAGFLG
                     IMYYFVPKQAERPVYSYRLSIVHFWALIFLYIWAGPHHLHYTALPEWASTLGMLFSVM
                     "
     gene            complement(889..1491)
                     /locus_tag="VIW27_05840"
     CDS             complement(889..1491)
                     /locus_tag="VIW27_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY5739226.1"
                     /translation="MFIQHIIGLFTDPTREWEKIRDQYKNQGKSSVGHVFVLAAIPAI
                     SGYIGTTQVGWRIGVGEPIRITGESAVSIAVIYYLALIVGVFTIGWVIHLLGKAYELE
                     KPLPLCIALATYTATPLFLIGIMEVYPVLWLNMLLGLPALAYTVYLLYSGLPILMEIP
                     AERGFLYASAVLAVGLIALVALLAMTALLWGMGLQPVFTH"
     gene            1645..>2726
                     /locus_tag="VIW27_05845"
     CDS             1645..>2726
                     /locus_tag="VIW27_05845"
                     /inference="COORDINATES: protein motif:HMM:NF023578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEY5739227.1"
                     /translation="MIDDTATRDQAALQTRPAESCFHCGEPLTPGSRFSACIDGVEQP
                     MCCPGCKAVAEVIVGAGHDHFYRVRDGHTPGASSSSPAGGDSAIFDRHEIQQRYVHQL
                     DENTREVSLILEGISCAACSWLIERYLGELPGVLEVRVNYATHHALLRWNPAQAKLGE
                     LLAAIDQIGYRAMPSDAGLQRASADRERRGLQRRLAVAGLFGMQVMMLSISLYAGAFS
                     GIDFEFEQLFRWLGMCLTLPVVIYAAAPFFAAAWRDLRRGGIGMDLPVSIAIGVAFAG
                     SVLATLRGSGEVYFDSVVMFVFFLTASRYFESMARHRSAATIERLVQSLPLVTTRLTA
                     DGDSGESVPAAALAVGDRVLVRPGETV"
BASE COUNT          531 a          804 c          888 g          503 t
ORIGIN      
        1 gcatgaccga gaacagcatg cccagggtcg aagcccactc cgggagggcg gtgtagtgca
       61 ggtggtgcgg accggcccag atgtaaagga agatcagcgc ccagaagtgg acgatggaga
      121 gccggtagga gtagaccggg cgctccgcct gcttgggcac gaagtaatac atgatgccga
      181 ggaaaccggc ggtcaggaaa aagccgaccg cgttgtgccc gtaccaccat tgcaccatcg
      241 cgtcctgcac cccggcgtag gccgagtagg atttccagaa gctcaccggc agcgccgcgc
      301 tgttgaacac atgcagcacc gcgacggtga cgatgaaggc accgaagaac cagttggcga
      361 cgtagatgtg gcgtatcttg cgcttcagga tggtgccgaa aaacaccagc gcgtaagaga
      421 cccagaccag cgtgatcaat atatcgatcg gccattcgag ctcggcgtat tccttgctgg
      481 tggtgatgcc gagcggcagc gtgatcgcgg ccagcaggat gaccagctgc caaccccaga
      541 aggtaaacgc cgccagtgcc ggaaaggcga gccgcgcgtg gcaggtgtgc tgcacgacgt
      601 agtatgaggt cgcgaacagc gcacagccgc caaaagcgaa gatcacggca ttggtgtgca
      661 atggccgcag gcggccgaag gtcagccacg gcaggtcgaa gttcagcacc ggccaggcca
      721 gttgcgcggc gattagcacg ccgaccgcca tgccgaccag gccccagaca atggtcatga
      781 tggagaactg ccggacaacc ttatagttgt aggtttcggt attgttcatt cggtgcatcc
      841 tcgatgtctg ctatgcatca aaaggggtaa gcccggccca gccggaaatc agtgggtgaa
      901 taccggctgc aggcccattc cccagagcag cgcggtcatc gccagcaggg caaccagcgc
      961 gatcaggccg accgcgagta ccgcggaagc atagaggaaa ccccgctcag cgggtatttc
     1021 catcaggatc ggcaatcccg agtacaacag gtaaacggtg taggcgagtg cgggcagtcc
     1081 caacagcatg ttgagccaca gtaccgggta gacttccatg atgccgatca ggaacagcgg
     1141 cgttgccgta taggtggcca gcgcaatgca cagcggcagg ggtttttcga gttcgtaggc
     1201 cttaccgagc agatgaatta cccagccgat ggtgaatacg ccgacgatca gtgccaggta
     1261 gtagatgacc gcgatcgaca ccgccgattc gccggtgatg cgaatcggtt cgccgacgcc
     1321 gattcgccag cccacctggg tggtacctat ataaccggaa attgccggaa tcgccgcgag
     1381 cacaaagacg tggccgacgc tgcttttccc ctggttttta tactggtcgc gaattttttc
     1441 ccactcgcgc gtgggatcgg tgaacagtcc gatgatatgc tggataaaca tgctggccca
     1501 atagtcatta ttgctggcat tatttccccc attttcgtgg gcaatttgat acatgtcaat
     1561 ggatgtacat ctagccgtaa cctcgccgtc gctcgcccgg gcgggtatgc gcatgcggcc
     1621 gggctgccgg caccagggga agcggtgatc gacgatactg ccacccgcga tcaagctgcg
     1681 ctccaaaccc gcccggcgga aagctgtttt cactgcggcg agccgcttac tccgggctcg
     1741 cgcttcagcg cgtgtatcga cggcgtcgag cagccgatgt gctgcccggg ctgcaaggcg
     1801 gtcgccgagg tgatcgtcgg cgccgggcat gatcatttct accgcgtgcg cgacgggcat
     1861 acgcccggag cgtcctcgag ctcgcccgct ggcggtgata gcgcgatttt cgatcgccac
     1921 gagatccagc agcgttatgt gcaccagctc gacgagaata cccgcgaggt cagcctgatt
     1981 ctcgagggta tcagttgtgc cgcctgcagc tggctgatcg agcgatatct cggcgaactg
     2041 ccgggtgtgc tcgaggtgcg cgtcaactat gccacgcacc acgctttgct gcgctggaac
     2101 ccggcgcagg caaaactcgg cgaattgctg gccgcgatcg atcaaatcgg gtaccgggcg
     2161 atgcccagcg acgccgggtt gcaacgcgcg tccgctgatc gcgagcggcg tggcctgcaa
     2221 cgccggctgg cggtcgcggg actgttcggc atgcaggtca tgatgctgtc gatcagcctc
     2281 tatgccggcg ccttcagcgg tatcgatttc gaattcgaac aactgttccg ctggctgggg
     2341 atgtgcctga cgctgccggt cgtgatttat gcggcggcgc cgtttttcgc cgccgcctgg
     2401 cgtgacctgc gacgtggcgg gatcggcatg gatctgccgg tgtcgatcgc tatcggcgtc
     2461 gcttttgccg gcagcgtgct ggccacgctg cgcggcagcg gtgaggtgta cttcgactcg
     2521 gtggtgatgt tcgtgttctt tctgaccgcc agccgttatt tcgaaagcat ggcgcggcac
     2581 cgcagcgcgg cgaccatcga gcggctggtg cagtcgctgc cgctggtgac cacgcggctg
     2641 acggccgatg gcgattccgg tgaatccgtg ccggcggcgg cactcgcggt cggtgaccgc
     2701 gtgcttgtac gccccggcga gaccgt
//
LOCUS       DATCZV010000262         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_126488, whole genome shotgun sequence.
ACCESSION   DATCZV010000262 DATCZV010000000
VERSION     DATCZV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126488"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1183
                     /locus_tag="VIW27_05850"
     CDS             41..1183
                     /locus_tag="VIW27_05850"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEY5739228.1"
                     /translation="MVSRHSPYPDELIEIASLEQAATARNEISRWPDYHATPLYRLDS
                     IARQIGVRGILYKDESQRFHLKSFKALGGAFAVARQLQQRIRQETGNSASIEDLLQRR
                     YPAIVENIVISCATDGNHGRSVAWGCQLFGCGCIIYIHRDVSEGRKQAMEAFGAEVIR
                     IDGNYDASVKQADADAQAHGRIIVSDTSYPGYMDIPRDVAIGYTVMLAEAVEQMQGEI
                     PTHVFIQAGVGGLAAAVCAYFWELWGERRPRFVVVEPEQANCLQQSAVSGKPVAVEGD
                     LETLMAGLSCGEVSLLAWQVLQTGADDFMTLSEDAVAPCMRLLAANDPAIEAGESAVA
                     GLGAAIAAREDRKMAATLGLDESSLVFVIGTEGATDPELYQQLVAS"
     gene            1183..>2350
                     /locus_tag="VIW27_05855"
     CDS             1183..>2350
                     /locus_tag="VIW27_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012600067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5739229.1"
                     /translation="MPAQQPERGFPQTEYESRTESAQRMMRERQLDAMLLTTEPHVRY
                     FSGFLTQFWQSPTRPWFLLLPATGKPIAVIPTIGVAGMRQTWIEDIRSWASPRPEDDG
                     ISLLLSTIRELPARFGRIGLTLGAESYLRMPARDYRRLEDELSTLELIDCAALMLKLC
                     SVKSAAEIEKIRYVCELASDSFNALPGFARRGQSEREIVQALRIDLLQRGADHVPYIV
                     SASGNGGYGDIIMGPGDRKLEDGDVLIIDTGSVYDGYYCDFDRNYAFGHAPDNARRAY
                     ETVYRSTDAGLAAARPGATTTDLWQAMWSVLEAGGATSNDVGRMGHGLGAQLTEWPSI
                     TASDNNPLEPGMVITLEPGMDCGENKMMVHEENIVITESGAKLLSKRAAAELPIV"
BASE COUNT          462 a          731 c          744 g          413 t
ORIGIN      
        1 gacaacactc tccgcccaat tgagccatat cagcaatccc atggttagcc ggcattcgcc
       61 ctaccccgac gaactgatcg aaattgccag cctagaacag gccgccacgg cccgtaacga
      121 gatttcacgc tggccggact accacgcgac cccgctttat cggctcgaca gtatcgcccg
      181 gcaaattggc gtcaggggaa tcttatacaa ggacgagtcg caacggtttc atttgaagag
      241 cttcaaggcg ctcggcggtg ccttcgccgt tgcgcgccag ctgcagcagc gaattcggca
      301 ggaaacgggt aactcggcct cgatcgagga tttgctgcag cgtcgttacc cggcaatcgt
      361 cgaaaatatc gttatcagtt gcgccaccga cggcaatcac gggcgctcgg tcgcctgggg
      421 ttgccagctg ttcggctgcg gctgcatcat ttatatccac cgcgacgtgt cggagggccg
      481 caagcaggcg atggaggcgt tcggcgccga ggtgattcgc atcgatggta actacgacgc
      541 ctcggtcaag caggccgacg ccgatgcgca agcccacggg cgtatcatcg tctccgatac
      601 cagctacccc gggtacatgg atattcctcg cgatgtcgcg attggctaca ccgtgatgct
      661 ggccgaggcg gtcgaacaga tgcagggcga gatcccgacg catgttttca tccaggcggg
      721 cgtcggcggg ctggccgccg cggtttgcgc ctacttctgg gaactctggg gcgagcgccg
      781 gccgcgcttt gtcgtggtcg agccggagca ggcaaattgc ctgcagcagt cagccgtatc
      841 gggcaagccg gtggccgtcg agggcgatct cgaaaccctg atggccggtc tttcctgcgg
      901 cgaggtttcg ctgctggcgt ggcaagttct gcaaacgggt gccgacgact tcatgacgct
      961 gagcgaagac gcggtcgcac cctgcatgcg cctgctcgcc gccaacgatc cggctatcga
     1021 ggccggtgaa tcggcggttg ccggcctggg ggccgcgatc gctgcccgcg aggaccggaa
     1081 aatggcggcc acgctgggac tggacgagtc cagcctggtc ttcgtgatcg gcaccgaggg
     1141 tgcaaccgat ccggaactgt atcaacagct ggtggcgagc tgatgcccgc gcagcagccc
     1201 gaacgcgggt ttccgcaaac cgagtacgaa tccaggaccg agagcgcgca acgtatgatg
     1261 cgcgaacgac aactcgatgc catgctgctc accaccgagc cgcatgtgcg gtatttcagc
     1321 ggcttcctga cccagttctg gcaaagcccg acccggccct ggtttttgct gctgcccgcg
     1381 accggcaaac cgatagcggt gataccaacc atcggcgttg ccggcatgcg tcaaacctgg
     1441 atcgaggata tccgcagctg ggcctcaccc cggcccgagg acgatgggat ttcgctgctg
     1501 ttgtcgacca tcagggagct cccggcgcgc ttcgggcgca tcgggctgac gctcggcgcc
     1561 gaaagctatt tgcgcatgcc ggcccgtgat taccggcggc tggaggacga gctgtcgacg
     1621 ctggaactga tcgactgcgc cgcgctgatg ctgaagctct gcagcgtgaa gtctgcggcc
     1681 gaaatcgaaa agataagata cgtctgcgaa ctggcgtcgg acagcttcaa tgccctgccc
     1741 ggtttcgcac ggcggggcca gagcgaacgc gaaatcgtac aggcattgcg catcgacctg
     1801 ctgcagcgtg gcgccgacca tgtgccatat atcgtctcgg cctcgggcaa cggcggatac
     1861 ggcgatatca tcatgggccc cggcgatcgc aaactggaag acggagacgt cctgattatc
     1921 gataccggca gcgtatacga cggctattat tgcgatttcg atcgcaacta tgcttttggt
     1981 cacgcgcctg ataacgcccg gcgggcatac gaaaccgtgt atcgcagcac cgatgccgga
     2041 ctggcagcgg cccgaccggg cgccaccacg accgacctgt ggcaggcgat gtggtcggta
     2101 ctcgaagccg gcggcgcaac cagcaacgat gtcggccgca tgggccacgg cctgggcgca
     2161 cagctcaccg aatggccctc gattaccgcc agcgataaca acccgctgga gcccgggatg
     2221 gtgataacgc tcgaacccgg catggactgc ggcgaaaaca agatgatggt gcacgaggaa
     2281 aacattgtca tcaccgaatc gggcgcaaaa cttctgagca agcgcgccgc cgccgaactg
     2341 ccgatcgtcg
//
LOCUS       DATCZV010000263         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_709452, whole genome shotgun sequence.
ACCESSION   DATCZV010000263 DATCZV010000000
VERSION     DATCZV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709452"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..2000
                     /locus_tag="VIW27_05860"
     CDS             18..2000
                     /locus_tag="VIW27_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5739230.1"
                     /translation="MTQYKNLFRPLELRHRRLDNRIVFGAHTANMSDQGLPGERHRGY
                     YEERAIGGAAMIVVEPMPVHATAVLTRGNFRHCDDEVVPYFRKITDAVHQHGTTILQQ
                     LYHVGQHGDSDLSFMPHWGPSGLQSYHDSDGSHAMRESEILEVIDCFTRAARRCQDAG
                     FDGVDLFAAYHALLDQFWTPWSNRRDDRWGGSLENRCRLSMSIINSIRDACGEDFIIG
                     LSTGYADNTPFVMTLEDFQEVIAYHDQSANLDYVSCGSGNYVDVDRIMPTFVYGEKQG
                     VPLAAALKQVVKHAKVTAESHIRTPENADYTIASGDSDLVSIVRGQIADPHWGNKTRA
                     NRHDDIRGCISCNQMCWGRRSRDYWISCLVNPSAGREFEWGGDRFEKTVQPRKVLVVG
                     AGPAGMEAARVAAERGHQVTLVEALGDLGGQFRLAGLAPRRGQVTELIGWYLRQFEQL
                     GVEVRYFTPMDEQDIIEFGADEVVLATGSMPDDQARQRWLPEAAELPGLSNGRVFPCE
                     EVFRDQAELDKSVIVIDEGGNWRGTGSAWFLAERGHEVTIVTPDPMIGKELTRTGSDF
                     QIRANLAKLGARFMLESVVEHWHGDRADVRSLLDGSVTSIEASAIVTATTNVVANDIE
                     LALSEAGIRLHVIGDAAAPRQAPYAFHDGRKIGLAL"
     gene            2014..>2175
                     /locus_tag="VIW27_05865"
     CDS             2014..>2175
                     /locus_tag="VIW27_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5739231.1"
                     /translation="MSDLGGINLAMQTPMHADGSIDYARWEELIDLYIDAGVHGLVLG
                     AGTGQHPYLT"
BASE COUNT          411 a          697 c          669 g          398 t
ORIGIN      
        1 gctgttaaga accagacatg acccagtaca aaaacctgtt ccggccgctc gaactgcgac
       61 accgcaggct cgacaaccgc atcgtgttcg gcgcgcatac cgccaacatg tcggaccagg
      121 gtctgcccgg agagcgtcat cgcggttact acgaggaacg cgctatcggc ggcgcggcaa
      181 tgatcgtggt cgaaccgatg ccggtgcacg ccaccgcggt gctgacgcgc ggtaatttcc
      241 gccactgcga cgacgaggtc gtcccctatt tccgcaagat taccgatgcc gtgcatcaac
      301 acggcaccac catcctgcag cagctttacc acgtcggcca gcacggcgat tcggatctgt
      361 cgttcatgcc gcactgggga ccgtccgggt tgcagtccta ccacgactcc gatggcagcc
      421 acgcgatgcg cgaatccgaa attctcgagg tcatcgattg cttcacgcgc gccgcgcgcc
      481 gctgccagga tgccggcttc gacggcgtcg acctgtttgc cgcctaccat gccctgctcg
      541 atcagttctg gactccgtgg tcgaaccggc gtgacgaccg ctggggcggc tcgctggaaa
      601 accgctgccg cctgtcgatg tcgatcatca actcgatccg ggacgcctgc ggcgaagact
      661 tcatcatcgg cctgtcgacc ggatacgccg ataacacgcc cttcgttatg acgctggaag
      721 acttccagga agttatcgcc tatcacgacc agagcgctaa cctggattat gtttcctgtg
      781 gctcgggaaa ttatgtcgac gtggatcgca tcatgccgac tttcgtctac ggcgaaaaac
      841 agggggtacc gctagccgcc gcgctcaagc aggtcgtcaa gcatgcgaag gtcacggcgg
      901 aaagccatat ccgtaccccg gaaaacgcgg actacacgat cgccagcggc gattccgacc
      961 tggtctcgat cgtgcgtggg cagatcgcag atccgcactg gggcaacaag actcgcgcta
     1021 accggcacga cgatattcgc ggctgcattt cctgcaacca gatgtgctgg ggacggcgct
     1081 cgcgcgatta ctggatctcc tgcctggtca acccttcggc cgggcgcgaa ttcgaatggg
     1141 gtggcgatcg tttcgaaaag acggtgcaac ccaggaaagt gctggtggtc ggtgccggcc
     1201 ccgccggtat ggaagccgct cgcgtcgctg cggagcgcgg tcaccaggtc acgctggtcg
     1261 aggcgctggg tgatctcggc ggccagtttc gcctcgccgg gctggcgccg cgacgcggcc
     1321 aggttaccga gctgatcggc tggtacctgc gacagttcga gcagctcggc gtggaagtgc
     1381 gctatttcac accgatggac gagcaggaca tcatcgagtt cggtgccgac gaagtcgtac
     1441 tcgctaccgg ctcgatgccc gacgaccagg cacgccagcg ctggctgccc gaggccgcgg
     1501 aactgcccgg cctcagtaac ggcagggtgt tcccctgcga agaagttttc cgcgaccagg
     1561 cggaactcga caagtcggtt atcgttatcg acgagggcgg taactggcgc ggcaccggta
     1621 gcgcctggtt cctggcggag cggggtcatg aagttaccat cgttacgccg gacccgatga
     1681 tcggcaagga gcttacgcgc accggctcgg acttccagat ccgtgcaaac ctggcgaagc
     1741 ttggcgctcg cttcatgctc gaatcggttg tcgaacactg gcacggcgac cgcgccgacg
     1801 tccgttcgct actcgatggt tcggttacga gcatcgaggc ctccgcgatc gtgaccgcga
     1861 ccaccaacgt cgtcgccaac gatatcgagc tggccctgag cgaagccggc atcagactcc
     1921 acgtaatcgg cgacgccgcg gcgccgcgcc aggctcccta cgcttttcac gacggccgca
     1981 agatcggcct ggcgttgtag ttcgagtcga atcatgagcg acctcggtgg aatcaatctc
     2041 gcgatgcaaa ccccgatgca tgccgacggc agcatcgact acgcccgctg ggaagaattg
     2101 atcgatcttt atatcgatgc cggcgtgcat ggcctggtgc ttggcgcggg taccggccag
     2161 catccctacc tgacc
//
LOCUS       DATCZV010000264         4628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_73592, whole genome shotgun sequence.
ACCESSION   DATCZV010000264 DATCZV010000000
VERSION     DATCZV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73592"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIW27_05870"
     CDS             <1..339
                     /locus_tag="VIW27_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY5739232.1"
                     /translation="GPQTPGELRARSGRLYQFDDNQAVVTTLEALNQLHAEPLVVRLP
                     RIPGRRDSEYMHLFGGPVDDLLEAQPSIEPPPAAAPRAAPARDPGIAELERRVSELES
                     EVARLKQLLD"
     gene            complement(457..1221)
                     /locus_tag="VIW27_05875"
     CDS             complement(457..1221)
                     /locus_tag="VIW27_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5739233.1"
                     /translation="MTRVDTGIARFGEAEWRQRVELAACYRAFVHYGWTDLVFTHLSA
                     RVPGQPQQYLINPYGLLFDEVCASNLIKVDFDGNVIDGGYPYNEAGHAIHTAMLKARP
                     DVNVVLHSHTRAGMAVSCMPCGLLPLTQQANEVRDLLCYHDYGIARIGSQECERLGED
                     LGERSIMIMKNHGLLTVGRSVAEAFYYLYTLENACKVQVDVLASGTGYVTPSDAIVDE
                     LACDGKAGAGSTPQHVELAWQAVLRLVERQAPSFRD"
     gene            complement(1218..2390)
                     /locus_tag="VIW27_05880"
     CDS             complement(1218..2390)
                     /locus_tag="VIW27_05880"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5739234.1"
                     /translation="MSSRIAKTEISPFLETLRTIAGKPYEQGYSLPGAFYTDDEWLQV
                     EREQLFARDWFCVGRIEEVAEPGDYFAFDFIGEPLLIVHGRDGVIRALSNVCRHRGTV
                     IADGRGNTRKFMCPYHHWAYDTAGQLLNAPHMDSHEAFDIKRCSLPELRCEFWQGFIF
                     VNLNPAAPSLVAQLGTLTDVIGNYHLEQMSMRYTADEVWPINWKCLLENFMEGYHLTP
                     LHKDTLHKVNPSRLCEHIEPGDYHFGYRVGFTARLPASHVGHPDLSEEEMNTCVMFAV
                     PPGLAVGIGSDYSSFLCLRPEATDSVRVKMGLIFHGDDWSQQDIDRAVELFQLTMAED
                     KEVLLRVQQGLASCFQQPGPLAARHLEGTIWDFYQYLSRGLAAPSPAAGRAPIGNA"
     gene            2485..3066
                     /locus_tag="VIW27_05885"
     CDS             2485..3066
                     /locus_tag="VIW27_05885"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY5739235.1"
                     /translation="MSTANVGGPEMADVSVGEANPYSAPDAALDTGQDALYKPKIIAF
                     NGRIGRMRYLAYSIGASFVLMLVTMPLIGMSVFTGAAMGGEAGMSMMTMLGMAVYYIG
                     AIVISVMYAKRRLNDLNRSGWWFLLFIVPIVNLLLVIYLLFFPGTGGANNFGPAPAAN
                     SIGVLILGWMLPAVFVIGIAAALLIPQFAGATP"
     gene            complement(3124..4161)
                     /locus_tag="VIW27_05890"
     CDS             complement(3124..4161)
                     /locus_tag="VIW27_05890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5739236.1"
                     /translation="MKFQLAINLERMDASIDMREVARHTLEMVQMADRAGFTTVWAAE
                     HHALEMTIAPNPFQILTWWAGHTDRIRLGTAVAVAAYWHPINLAGEAAMLDLISGGRL
                     EFGIGSGAYQREFDRMHPGLKQSDAYRYLHEMLPALKALWAGDYAHDGEFWSFPTSTS
                     VPKPLQQPHPPIWVAARSPITFDYAVANNCHVMSWPLTRPFTEAELYRRQLDEALAAH
                     PDAARPTFALMRHTAVYEDSAGRDAAIRALQGVLGQFENLFRNLGDVDNGFPKRIPLE
                     ELSDREQYNPEMLEDNLMFGKPEAVIEKLRRYEALGVDEFIYYASMGLGQQQQKRSLE
                     LFCSEVMPAFA"
     gene            complement(4309..>4628)
                     /locus_tag="VIW27_05895"
     CDS             complement(4309..>4628)
                     /locus_tag="VIW27_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5739237.1"
                     /translation="ETASIERVRGWVLANDPPVYALCRRVLAFGVTELVRPEPPIRVP
                     TLVMTCEHDSGSTSEMSQTIAGEIDGAQVIIVPGLRHMGLVENPRFFVTAIDEFLETI
                     RQL"
BASE COUNT          850 a         1467 c         1441 g          870 t
ORIGIN      
        1 ggcccgcaga cccccggcga gctgcgcgcg cgcagcggca ggctgtacca gttcgacgac
       61 aaccaggccg tggttacgac actcgaagcg ctcaaccagt tgcacgccga accgctggtg
      121 gtcaggctgc cgcgaattcc ggggcgcagg gattccgagt atatgcacct gtttggcggg
      181 ccggtggacg acctgctcga ggcgcaaccg tcgatcgagc cgccgcctgc ggccgcaccg
      241 cgagcggcgc cggctcgaga tccgggtatc gcggaactcg agcgccgcgt cagcgagctc
      301 gagagcgagg tcgcgaggct taagcaattg ctcgattaac cacccttccc ggctggcggc
      361 gaactgcgac gagcggtgcg catggccgcg ttagcgcggc gtgtcgataa aacgcctgaa
      421 cgcccaccgg gtgacgcgac cgccgccgcc caaacactag tccctgaacg acggcgcctg
      481 tcgctcgacc aggcgcagca ccgcctgcca ggcgagttcg acgtgctgcg gcgtgctgcc
      541 ggcgccggcc ttgccgtcgc acgccagttc gtcgacgatc gcgtcactcg gcgtcacgta
      601 gccggttccc gaggcgagca cgtccacctg caccttgcag gcgttctcca gcgtgtacag
      661 gtagtaaaag gcttcggcga cactgcggcc gacagtcaac agcccgtgat tcttcatgat
      721 catgatcgag cgctcgccga gatcctcgcc gagacgttcg cattcctggc tgccgatgcg
      781 cgcgatgccg tagtcgtgat agcagagcag atcgcggacc tcgttggcct gctgggtcaa
      841 cggcagcagc ccgcagggca tgcacgatac cgccatgccg gcgcgcgtgt ggctgtgcag
      901 cacgacattg acatccggcc gcgccttcag catcgcggta tggatcgcgt gaccggcctc
      961 gttgtaaggg taaccgccgt cgatgacatt gccgtcgaag tcgaccttga tcaggttgct
     1021 ggcgcagact tcgtcgaaca gcagtccgta gggattgatc aggtattgct gcggctgccc
     1081 cggtacgcgc gcgctcaggt gggtaaagac caggtcggtc cagccataat gcacgaaggc
     1141 ccggtagcag gcagcgagct cgacgcgttg ccgccattcc gcctcgccaa agcgcgcgat
     1201 cccggtgtcg acgcgggtca cgcgttgccg atcggcgccc ggccggcggc cgggctcggt
     1261 gccgcgagtc cgcggctcag gtactggtaa aagtcccaga tggtaccctc gaggtgacgc
     1321 gccgccagcg gccccggctg ctggaagcac gacgcgagcc cctgttgcac gcgcagcaac
     1381 acttccttgt cctcggccat ggtcagctga aacagttcga ccgcgcggtc gatgtcctgt
     1441 tgtgaccagt cgtcgccgtg aaagatcagt cccatcttta ccctgacact gtccgtggct
     1501 tcgggacgca ggcacaggaa agaactgtaa tcgctgccga taccgaccgc gagacccggc
     1561 ggcaccgcga acatcacgca ggtattcatt tcttcttccg aaaggtccgg gtgaccgacg
     1621 tgactggcgg gaaggcgcgc agtgaagccg acgcgatagc cgaaatgata atcgccgggc
     1681 tcgatgtgct cgcacagccg gctcgggttg accttgtgca gcgtatcctt gtgcagcggc
     1741 gtcaggtggt agccctccat gaagttttcc agcaggcatt tccagttgat cggccagact
     1801 tcgtcggccg tgtaacgcat cgacatctgc tcgaggtgat agttgccgat cacatcggta
     1861 agcgtgccga gttgcgccac caggctcggc gccgccgggt tcagattgac gaagatgaat
     1921 ccctgccaaa actcgcagcg caattccggc aaactgcagc gcttgatgtc aaaagcctcg
     1981 tgggaatcca tgtgcggtgc gttcagcaac tggccggcgg tatcgtaagc ccagtgatga
     2041 tacggacaca tgaatttgcg cgtgttaccg cggccatcgg cgattaccgt gccgcggtgc
     2101 cggcagacgt tcgacagcgc gcgtatcacg ccgtcgcggc cgtgcacgat cagcagcggt
     2161 tcgccgatga aatcgaaggc gaaataatcg ccgggttcgg cgacttcctc gatgcgcccg
     2221 acacaaaacc agtcgcgcgc gaacagttgt tcgcgttcga cttgcagcca ctcgtcatcg
     2281 gtgtaaaagg cgcctggcag cgagtaaccc tgttcgtagg gcttgccggc aatcgttcgc
     2341 agggtttcca ggaacggtga aatctctgtt ttcgctatcc tcgacgacat ggcggtctcc
     2401 ggcccaagat gcaaattatc gttgtcaagc tgacaccttg gcctatatta aataactgca
     2461 aactaataga acaggggaaa tagcatgagc actgcaaacg tgggcgggcc cgagatggcg
     2521 gacgtcagtg taggcgaagc caacccttac tcggctcctg acgccgcgct ggataccggg
     2581 caagacgcgt tatacaaacc gaaaatcatc gccttcaacg gccgtatcgg acgtatgcgc
     2641 tacctggcct acagtatcgg tgccagtttc gtgctgatgc tggtgacgat gccgctgatc
     2701 ggcatgtcag tcttcaccgg tgccgcaatg ggcggcgaag ccggaatgtc gatgatgacc
     2761 atgctgggca tggctgtcta ttatatcggc gcgatcgtga tctcggtcat gtacgccaaa
     2821 cggcggctga acgatctgaa ccgcagcggc tggtggtttc tgttgttcat cgtaccgatc
     2881 gtgaacctgt tgctggtgat ctacctgctc ttctttcccg gcaccggcgg agccaataac
     2941 ttcggacccg cgccggcggc gaattcgata ggcgtactga ttctcggctg gatgctgccg
     3001 gcagtgttcg tcatcggtat cgctgccgcg ctcctgattc cgcaattcgc cggcgcgact
     3061 ccctgacgga acccacccct cgatgccagc cgccagcgcg cagccggcgt cgccgcggct
     3121 cgctcaggca aaggccggca tgacctcgga gcaaaacagt tccagcgacc gcttttgctg
     3181 ctgctgcccg agtcccatgc tggcatagta aatgaactcg tcgacgccca gcgcctcgta
     3241 gcgcctgagc ttttcgatca ccgcctccgg cttgccgaac atcaggttat cctcgagcat
     3301 ctcgggattg tattgctcac ggtcggacaa ctcttccagc ggaatacgtt tcgggaaacc
     3361 gttgtcgaca tcgccgaggt tgcgaaacag gttctcgaac tggccgagca ccccctgcaa
     3421 ggcccgaatc gcggcatcgc gtccggcgct gtcctcgtaa accgcggtat gacgcattag
     3481 cgcaaaagtc ggccgcgcgg catcggggtg cgcggcgagc gcctcgtcga gctggcggcg
     3541 ataaagttcg gcctcggtaa acggacgcgt caacggccac gacatgacgt ggcaattatt
     3601 cgcgaccgcg tagtcgaagg tgatcggcga gcgtgccgcg acccagattg gcgggtgcgg
     3661 ctgctgcaac ggcttgggaa ccgaggtcga ggtcgggaac gaccagaatt cgccgtcgtg
     3721 cgcgtaatcg ccagcccaca gggctttcag cgccggcagc atttcatgca ggtaacgata
     3781 ggcatcgctt tgcttgaggc ccgggtgcat gcgatcgaat tcgcgctggt aggcgcccga
     3841 accgatcccg aactcgagac gcccgccgct gatcaggtcg agcatcgcgg cttctccggc
     3901 gaggttgatc gggtgccagt aggccgctac cgcgaccgcc gtaccgaggc ggatccggtc
     3961 ggtatggcct gcccaccagg tcaggatctg gaacggattg ggcgcgatgg tcatttccag
     4021 cgcatggtgc tcggccgccc acacggtggt aaatcccgcg cggtccgcca tctgcaccat
     4081 ctccagcgtg tgccgcgcca cctcgcgcat atcgatgctc gcgtccatgc gctccaggtt
     4141 gatcgccagt tgaaatttca ttttcgttcg cctcgtgttt acgctgccgc tcgccgggta
     4201 acggctttgc catgttaaac gatttttccc ggcctgctac tccctcgtgg gttacgccgt
     4261 gttgccgcgt aacccgccga cggcaccgac gggacggccg ctgtcgagtc aaagctggcg
     4321 aatcgtctcc agaaattcgt cgatcgcggt aacgaaaaac cgcggatttt cgaccagccc
     4381 catgtgccgc aagccgggaa cgatgatcac ctgtgcgccg tcgatttcgc cggcaatggt
     4441 ctggctcatc tccgacgtgc tgccgctgtc gtgctcacag gtcatgacca gcgtcggtac
     4501 gcggatcggc ggctcgggcc gcaccagttc agtgacgccg aacgccagca cgcgacggca
     4561 cagcgcgtag actggcgggt cgttggccag cacccagccc cgaacccgct cgatggaggc
     4621 ggtctcac
//
LOCUS       DATCZV010000265         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_391813, whole genome shotgun sequence.
ACCESSION   DATCZV010000265 DATCZV010000000
VERSION     DATCZV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391813"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="VIW27_05900"
     CDS             complement(<1..1789)
                     /locus_tag="VIW27_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY5739238.1"
                     /translation="MSELSLKPGFSLGSYPQRPESTLGEFETAVMRTLERLVKPGRRI
                     VRPWGVVRRVRRVEQQLTALSDAELDAAITELRGRLQRHGLRKALVVQAFAMVCEAAG
                     RTLGMWHYDEQVYGGWLMMHGMLAEMQTGEGKTLATTLPACAAALAGVPVHVITANDY
                     LATRDCELMTPLYRRLGLEGSAVINQMPRDVCKRAYQADIVHTTNKQIAFDYLRDRIE
                     MGEDTGDLRFQYRQIKDRLAAGSDNPLLLRGLCFAIVDEADSVLIDEANTPLIITRMV
                     EDDDSPETYSDALYLASLLRADRDYRADSRLRSVELTVAGEDCIEEQIHNLPKLWNNQ
                     RKRVSLVRQALAALLFYHRNRDYLVEDGKVEIIDQSTGRRMPDRAWEQGLQQMIEAKE
                     GCLISERREPQARISYQRFFSRYLRLGGTSGTIGEVRGELYRVYGLEVRSVATHLPSK
                     RRFLGRRLYRDKVAQQTALMERVTQMQTVGRPILIGTTSVEESERVSGWLSKSGIEHR
                     VLNARQDQEEAEIVAAAGQQGAITVATNMAGRGTDIALGAVVEGLGGLHVIAASLNPS
                     ARLDRQLYGRCARQGDSGSAEAILSLEDEA"
     gene            complement(1782..3926)
                     /locus_tag="VIW27_05905"
     CDS             complement(1782..3926)
                     /locus_tag="VIW27_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5739239.1"
                     /translation="MDNVLHSPHWYRVAPLKPRLHDQVDIHRHDYRGQIWYLLENTTT
                     GRSHRFNPAAYRFIGLMDGERSIDEIHRLLIDSDETTAPGQVEIIELLGNLYAADLLK
                     SGALADTGELFERQSKMRGRKFAQRFANPVALRVPMWDPERFLNRHFNKVRWVFSKWM
                     GQLWILLLIYAATEAAQNWPRIGHHFEINALTPYNLLLLFLLYPPIKFVHELGHAFSA
                     KLEGGEVHEMGITFLVFVPVPYVNVSAATHFRSRYKRILVSAAGIMIESFIAALGLLL
                     FLAAQPGLVQNIGFNMFLIGGISSLFFNGNPLLKYDGYYMLADAAGIPNLYQRSAQYW
                     QYLMQRYLFGLREALSPVSARGEAAWFAVYSPLSLAYRLGILWFIVIMVTDKFFYLGV
                     LLAIWMVGMQIMLPLYKALMFLLSSPALQAKRNRALATSGLLSLAVAAIFGFFPVPSY
                     SVNQGVVWQPNEVHLRAQHGGFVEQLLVDNHQRITEGTPILRLSDPLLSSQAKIARAR
                     VEEMTSRYRASRASNHFEAGIVGEDLQVAKSEFDYILVKAEQMSVNAFKSGRLLLLEA
                     DDLPGRYVEKGDLLGYILDDEKPTVRMVVSQQNIGLLQQRVVDIKVRFATDPGTEFDA
                     SILRQAPEATNRLPSLALATTGGGPFVVTAADGGYSTLEKVFMVDLEPDFRGRQVPLG
                     TRAWIRVDHGSEALATQWYRRLRQVFLRQLNV"
     gene            complement(3939..>4287)
                     /locus_tag="VIW27_05910"
     CDS             complement(3939..>4287)
                     /locus_tag="VIW27_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY5739240.1"
                     /translation="ETLFKIAPLEDYRIIVKVDESEIAYIEPGQRGSLALSSLSEQQL
                     PITIERITSVARAEDGANFFRVEASLPEGGKALRPGMEGIAKINAGQERLLWIWSHEI
                     IDWLRLWVWSWWP"
BASE COUNT          810 a         1354 c         1327 g          796 t
ORIGIN      
        1 gtgcctcgtc ctcgagcgac agaatcgcct cggcactgcc ggaatctccc tggcgggcac
       61 agcggccgta caactggcga tcgagacgcg ccgacgggtt caggctggcc gcaatcacgt
      121 gcaggccgcc gagaccttcg accaccgcgc cgagcgcgat gtcggtgccg cggccggcca
      181 tgttagtggc cacggtaatt gccccctgct ggcccgcggc ggcgacgatc tccgcctctt
      241 cctggtcctg cctggcattg agcacgcggt gttcgatgcc gctcttgctc aaccagccgg
      301 acactcgttc cgactcctcg accgaagtgg tgccgatcag gatcgggcgg ccgacggttt
      361 gcatctgcgt tacccgctcc atcagcgcgg tctgttgcgc gaccttgtcg cggtacaggc
      421 gacgcccgag aaagcgccgt ttcgacggca gatgagtggc gacgctgcgc acctcgagac
      481 catagacacg gtacagttcg ccacgcacct cgccgatggt gcccgaggtg ccaccaaggc
      541 gcaggtaacg gctgaagaat cgctggtaac tgatacgcgc ctggggttcg cgccgctcgc
      601 tgatcaggca accttccttg gcttcgatca tttgctgcaa gccctgctcc caggcgcggt
      661 ccggcatgcg ccggccggtg gattgatcga tgatctcgac cttgccgtcc tcgaccaggt
      721 aatcgcggtt gcgatggtag aacagcagcg ccgcgagcgc ctggcgcacc agtgagacgc
      781 gcttgcgctg gttgttccac agcttcggca ggttatgaat ctgctcctcg atacagtctt
      841 caccggccac ggtcagctcc accgaacgca gccgcgaatc ggcgcggtag tcgcggtccg
      901 cccgcagcag tgatgccagg tagagggcgt cgctgtaggt ttccggggaa tcgtcgtcct
      961 cgaccatacg ggtgattatc agcggcgtgt tggcctcgtc gatcaatacg ctatcggcct
     1021 cgtcgacgat cgcgaagcac aggccgcgca acagcagcgg attgtcgctg ccggcagcca
     1081 gccggtcctt gatctgccgg tactggaaac gcaggtcgcc ggtatcctca cccatctcga
     1141 tacggtcgcg caggtaatcg aaggcgatct gcttgttagt cgtgtgcacg atatcggcct
     1201 ggtaagcacg cttgcaaaca tcgcggggca tctggttgat caccgcactg ccttccaggc
     1261 cgaggcgccg gtagagcggc gtcatcaatt cgcagtcgcg cgtggcgagg tagtcgttgg
     1321 cggtgatcac gtgtaccggg acgcccgcca gcgccgcggc gcaggccggc agtgtcgtcg
     1381 ccagcgtctt accctcaccg gtctgcattt cggcgagcat gccgtgcatc atcagccagc
     1441 cgccgtaaac ctgctcatcg taatgccaca tgccgagggt gcgtccggcc gcctcgcaga
     1501 ccatcgcgaa tgcctggacg acgagcgcct tgcgcaaacc atggcgctgc agtcggccgc
     1561 gcaattcggt gatcgccgca tccagctcgg catcgctgag ggcagtcagc tgctgctcga
     1621 cgcgccgcac ccggcgcacc acgccccacg gccgcacgat ccggcgtcca ggcttcacca
     1681 ggcgctccag ggtacgcatg acagccgttt cgaactcgcc cagggtcgac tccgggcgct
     1741 gcggatagct gccgagactg aagccgggtt tcaacgataa ttcagacatt gagctgcctc
     1801 agaaatactt gccgcaagcg ccggtaccac tgtgtcgcca gcgcctcgct gccgtgatcg
     1861 acgcgtatcc aggcgcgcgt gccgagcggc acctggcgac cgcgaaaatc aggttcgagg
     1921 tcgaccatga ataccttttc gagggtcgaa tagccgccat cagcggcggt gaccacgaat
     1981 ggaccgccac cggtagtggc cagcgccagg ctcggcaagc gattggtggc ctcgggcgcc
     2041 tggcgcagaa tactggcgtc gaactcggtg ccgggatcgg tcgcgaagcg caccttgatg
     2101 tcgacgaccc gctgctgcag caggccgatg ttttgctggc tgacgaccat gcgcacggtc
     2161 ggcttctcgt cgtcgaggat atagccgagt aaatcaccct tctccacgta gcggcccggc
     2221 agatcgtcgg cctcgagcaa cagcagccgg ccgcttttga acgcgtttac cgacatctgc
     2281 tcggccttga cgagtatgta atcgaactcg gatttcgcga cctgcaggtc ttcgccgaca
     2341 atgccggcct cgaagtggtt gctggcgcgg ctggcgcggt aacgggaagt catttcctcg
     2401 acccgcgcgc gtgctatctt cgcctggctg ctgaggagcg gatcgctgag cctgagtatc
     2461 ggcgtaccct cggtgatgcg ctgatggttg tcgaccagca attgttcgac gaagccgccg
     2521 tgctgcgcac gcaagtgcac ttcgttgggc tgccagacca ctccctggtt gacgctgtag
     2581 gaaggcaccg ggaaaaatcc gaagatcgcg gccaccgcaa gcgacaacag gccactggtc
     2641 gctagcgcgc ggttgcgttt tgcctgcagt gccgggctcg acagcaggaa catcagcgcc
     2701 ttgtaaagcg gcaacatgat ttgcatgccc accatccaga tcgccaacag cacgccgaga
     2761 taaaaaaact tgtcggtgac catgatgacg ataaaccaca ggatgccgag ccggtatgcc
     2821 agtgacagcg gcgagtaaac ggcaaaccag gccgcctcgc cgcgagcgct gaccggcgac
     2881 agagcctcgc gcaggccgaa caggtaacgc tgcatcaggt attgccagta ctgcgccgag
     2941 cgctggtaga gattgggaat gccggcggca tcggcgagca tgtaatagcc gtcatatttc
     3001 aacaacgggt tgccgttgaa gaacagcgac gatataccgc cgatcagaaa catgttgaag
     3061 ccgatattct gcaccagccc gggctgggcg gcgagaaaca gcagtaatcc gagcgccgcg
     3121 ataaacgact cgatcatgat accggcggca ctgaccagga tgcgcttgta gcgactgcga
     3181 aaatgggttg ccgccgagac gttgacatag ggcaccggca cgaacaccag gaaggtaatg
     3241 cccatttcgt ggacttcccc gccctccagc ttggcgctga aggcatggcc cagttcgtgg
     3301 acgaacttga tcggtggata caacaggaat aacagcagca ggttgtatgg cgtcagggca
     3361 ttgatttcga aatggtggcc gatgcgcggc cagttctgcg cggcctcggt cgcggcgtag
     3421 atcagcagca gtatccacaa ctgccccatc cacttgctga aaacccagcg caccttattg
     3481 aagtggcgat tcagaaatct ttccgggtcc cacatcggga ccctcagcgc gaccgggttc
     3541 gcgaagcgct gcgcgaactt gcggccgcgc attttcgact ggcgctcgaa tagctcaccg
     3601 gtatcggcaa gcgcaccact cttcagcagg tcggcggcat acaggttgcc cagcagttcg
     3661 ataatctcta cctgaccggg cgccgtcgtc tcgtcgctgt cgatcagcag gcggtgaatt
     3721 tcatcgatac tgcgctcgcc gtccatcagg ccgatgaagc ggtaagcggc ggggttgaaa
     3781 cggtgactgc ggccggtggt ggtgttttcg agcaaatacc agatctggcc gcggtaatcg
     3841 tggcggtgga tatcgacctg gtcgtgcagc ctcggtttca gcggcgcgac ccggtaccag
     3901 tgcgggctat gtaatacgtt atccatcgcc gcgctccgtc acggccacca cgaccagacc
     3961 cagagtctga gccagtcgat gatttcgtgt gaccatatcc acagcagccg ttcctgcccg
     4021 gcgttgatct tggcaatccc ctccatgccc ggccgcagtg ccttgccacc ctcgggcagg
     4081 gaggcctcga cgcgaaagaa attggcgcca tcctcggcgc gggcgaccga ggtaatccgt
     4141 tcgatcgtga tcggcaattg ctgctcggag agactcgaca gggccagcga accccgctgt
     4201 ccgggctcga tataggcgat ctcgctctca tcgaccttga cgattatgcg gtagtcttcc
     4261 agcggcgcga tcttgaacag cgtttca
//
LOCUS       DATCZV010000266         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_339238, whole genome shotgun sequence.
ACCESSION   DATCZV010000266 DATCZV010000000
VERSION     DATCZV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339238"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1270)
                     /locus_tag="VIW27_05915"
     CDS             complement(65..1270)
                     /locus_tag="VIW27_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5739241.1"
                     /translation="MKRRLDKSRILIYSHDSFGLGHLRRCRSIAHSLVSRFKGLSVLI
                     LSGSPIIGSFDFKARVDFVRIPGIIKLRDGAYTSLALHIDTEQTLRMRESIIYHTAAC
                     FEPDLFLVDKEPTGLQGEVTSTLKLLKQMGTVNVLGLRDIMDEPSKLRAEWQRKQVLP
                     VLEELYHEIWVYGLSDMCNPVEEQQLSTSIANKITYTGYLGRDIPSDRNWVPQTSLDE
                     PFILVTAGGGGDGVEMVDWVLRAYEADRDQPLRAIIVTGPFMPPANQQEFHERCEQLD
                     NVEILTFDSHIEILMQKSAAIVAMGGYNTFCEILTLDKPALIVPRSYPRQEQLIRAQR
                     AVRLGLVSMLDPAGERDPRQMAETLRNLPQQSKPSSRQIPGLLSGHQSIAEIVRERLE
                     TPEPEIVSA"
     gene            1420..2778
                     /locus_tag="VIW27_05920"
     CDS             1420..2778
                     /locus_tag="VIW27_05920"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5739242.1"
                     /translation="MNSNSPISPAADERPASSLKLRNKLLPQRLRRSIQEQQERSEIL
                     ISVIQLVIVTVFGLLYAVSPKTFSQDADFAPVPWVLSLYLGFSLIRLALAVKRKLPDW
                     MLYLSSVVDIALLMGLIWSFHLQYEQPPSFYLKAPTLLYLFIFIAIRALHFDPRFVVS
                     TGISAVVGWSLLVCYVMFSDPTNNMVTRNYVEYMTSNSVLIGAEFDKIISILMVTGVL
                     ALALYRARNLLIESVIEGSAARDLSRFVPEEVAQKVIQSEEGAITGKGEVSECTILFT
                     DIEGFTSISEILSPEQLIEALNRYFTLIAVPITRYGGVISQFQGDAVLATFNVPRPDS
                     EHAANAIRAALEIQAVLRGEEFGDGVHFNTRIGINTGPVVGGLVGSGDRVGYTVHGEN
                     VNLTARLEQLNKDYGTRIIVADSTRRQVADGLFDFRELDEVAVRGLNRPVRIFTIENG
                     GE"
     gene            complement(2800..3453)
                     /locus_tag="VIW27_05925"
     CDS             complement(2800..3453)
                     /locus_tag="VIW27_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739243.1"
                     /translation="MPPRGVPNLIDPGRFRALWQRCLVDGADDDSAAIHQRLLDGYRE
                     PQRCYHTLQHIEHCISVFDECRSLVDNPDALELAIWFHDVIYEPGESDNEARSAQLYL
                     QLSDGVHADAARALVERLIMATLHLGCSLADGDASYMVDIDLSSFGLPWDEFLRDSEN
                     LRREANHLDDAEYHLKARAFHHSLRSRERFFYTDFFAERFEARAHRNIERYIECIEN"
     gene            complement(3454..>3767)
                     /locus_tag="VIW27_05930"
     CDS             complement(3454..>3767)
                     /locus_tag="VIW27_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5739244.1"
                     /translation="YVIMQGAVDIVTETDAGPIVSGTLTQNQLIGELGLLNNAPRNAT
                     LVTNGTLRVMKITGDMFFRILRENSDVALDVIRMLSDKLSATHERVEILQKELNQQDD
                     S"
BASE COUNT          707 a         1092 c         1178 g          790 t
ORIGIN      
        1 cgatgccgag caggtgctgg acatgaatca gaatgcgatg gggcgagtcg cgcgacaccc
       61 gaattcaggc gctgacgatc tcgggctccg gggtttccag tctttcgcgg acgatctcgg
      121 cgatactctg gtggccggaa agcagtccgg ggatttgccg ggaagagggc ttcgactgct
      181 gcggcaggtt gcgcaaggtt tcggccattt gcctcggatc gcgctctccc gccggatcga
      241 gcatactcac caggccgagg cgcacggcgc gctgagcgcg aatcagctgt tcctggcgcg
      301 gataggagcg gggcacgata agtgccggct tgtcgagtgt cagaatttcg caaaaagtat
      361 tgtaaccgcc catcgcgacg atcgcggcgg acttttgcat caggatttcg atatggctgt
      421 cgaaggtcag gatttcgacg ttgtccagct gctcgcagcg ctcgtgaaat tcctgctggt
      481 tggccggcgg catgaacgga ccggtaacga tgatcgcgcg cagcggctga tcgcggtcgg
      541 cctcgtaggc gcgcagcacc cagtcgacca tctccacgcc atcgccaccg ccgccggcgg
      601 taaccaggat gaagggttcg tcgaggctgg tctgcggtac ccagttgcgg tcgctcggaa
      661 tatctcgccc caggtagccg gtgtaggtga tcttgttggc gatcgacgtc gataactgct
      721 gttcctcgac cgggttgcac atgtcgctga gtccgtatac ccagatttcg tggtagagct
      781 cctcgagtac cggcagcacc tgcttgcgct gccactccgc ccgcagcttc gacggctcgt
      841 ccatgatatc gcgcaagccg agtacattga ccgtgcccat ttgcttgagc agcttcagcg
      901 tgctggtgac ctctccctgc agaccggtcg gttccttgtc gaccaggaac aggtcgggtt
      961 cgaagcaggc ggcagtatgg tagatgatcg actcgcgcat tcgcagggtt tgctcggtgt
     1021 cgatatgcag ggccagcgag gtgtaggcac cgtcgcgcag cttgatgata ccgggaatgc
     1081 gcacgaaatc gacgcgcgcc ttgaagtcga agctgccgat gatcggtgag cccgacagga
     1141 tcagtaccga caggcccttg aaacgcgata ccagcgagtg ggcgatcgag cggcagcgcc
     1201 gcagatgccc caggccaaac gagtcgtggc tataaatcag gatcctggat ttatccagcc
     1261 gtctcttcat tgaatgcgcg tcttgctgcg tacggcgccc agactacacc gaaatgtcct
     1321 cgaacgcact gcccgggacg gtatttttga tcccgcccaa attgttgaca agatttcctg
     1381 tccaaggtgc ataatgcaga aatcgatatc ggagcccctg tgaacagtaa ttcgccgatc
     1441 tccccggccg ccgacgagcg gcccgcgagc agcctgaaac tccgcaacaa gttactgccg
     1501 cagcgcctgc ggcgctcgat ccaggagcag caggagcgca gtgaaatact gatcagtgtc
     1561 attcagctcg tgatcgtcac cgttttcggc ctgctttatg cggtttcacc gaagactttc
     1621 tcgcaggatg ccgatttcgc gccggttccg tgggtattgt ccctgtacct gggtttcagc
     1681 ctgattcggc tggcgctggc ggttaagcgc aagctgcccg actggatgct gtacctgtcg
     1741 tcggtggtcg acatcgcgct gctgatggga ctgatctgga gcttccacct gcaatacgag
     1801 cagccgccgt ctttttacct gaaagcaccg acgctgctgt acctgtttat ctttatcgcc
     1861 attcgcgcgc tgcatttcga cccgcgcttc gtcgtctcga ccggtatcag tgcggttgtc
     1921 ggctggtcgc tgctggtctg ttacgtgatg ttcagcgacc ccaccaataa catggtgact
     1981 cgaaactacg tcgagtacat gacctcgaac agcgtgctga tcggcgccga gttcgacaag
     2041 atcatctcca tactcatggt caccggggtg ctggcgctgg ccctgtaccg cgcccgcaat
     2101 ttgctgatcg aatcggtcat cgagggcagt gccgcgcgcg acctgtcgcg cttcgtgccc
     2161 gaggaagtgg cgcaaaaggt catccagtcg gaagagggcg cgattaccgg caagggcgag
     2221 gtcagcgaat gcacgatttt gtttaccgat atcgagggct ttacctcgat cagtgaaatt
     2281 ctctcgccgg agcagttgat cgaagcactc aaccgttatt tcacgctgat cgcggtgccg
     2341 attacgcgtt acggtggggt gatcagccag ttccagggcg atgcggtgct ggcgacgttc
     2401 aatgttccgc gaccggatag tgagcacgcc gccaacgcga ttcgcgcggc actggaaatc
     2461 caggcggtac tgcggggcga ggaattcggc gacggcgtac atttcaacac ccgcatcggt
     2521 atcaataccg ggcccgtggt cggcgggctg gtcggctcgg gcgatcgggt cggctacacc
     2581 gtgcacggcg aaaacgtcaa cctgacggcg cggctcgaac agctcaacaa ggattacggc
     2641 acgcgcatca tcgtcgccga tagcactcgc cggcaggtgg ctgatggcct gttcgatttt
     2701 cgcgaactcg acgaagtcgc ggtgcgcggc ctcaatcggc cggtacgaat cttcaccatc
     2761 gagaatggcg gcgaatagac cggccgccag gcgccggcat cagttttcga tgcactcgat
     2821 gtagcgttcg atattgcggt gcgcgcgggc ttcgaaccgc tcggcaaaaa aatcggtata
     2881 gaaaaagcgc tcccgggagc gcaggctgtg gtgaaaggcc cgcgccttca gatgatattc
     2941 ggcgtcgtcg aggtggttgg cctcgcgccg cagattttcg ctgtcgcgca ggaattcatc
     3001 ccacggcaaa ccgaaactcg acaggtcgat gtcgaccatg tagctcgcat ccccgtccgc
     3061 gagcgagcag ccgaggtgca gcgtcgccat gatcaaccgt tcgaccagcg cgcgggccgc
     3121 atcggcgtgg acgccgtccg acaattgcag atagagctgc gcactgcgcg cctcgttgtc
     3181 ggattcgccg ggctcgtaga tgacgtcgtg aaaccagatc gcgagttcca gcgcgtcggg
     3241 attgtcgacc agggacctgc attcgtcgaa caccgaaatg cagtgttcga tgtgctgcag
     3301 cgtgtggtag cagcgttgcg gttcgcggta gccgtcgagc agccgctggt gaatggccgc
     3361 gctatcgtcg tcggccccgt cgaccaggca gcgttgccac agtgctcgaa atcgacccgg
     3421 atcgatgagg tttggcaccc cgcggggcgg catttaactg tcgtcctgtt gattcagctc
     3481 tttttgcagg atctcgacgc gctcgtgggt ggccgataac ttgtcgctga gcatgcgaat
     3541 gacgtccagc gcgacgtcgg aattttcgcg caatatgcga aagaacatgt cgccggtaat
     3601 cttcatcacc cgcagtgtgc cgttggtgac cagggttgca ttgcggggcg cattgttgag
     3661 caggccgagt tcgccgatca actggttttg tgtcagggta ccggaaacga tcggtccggc
     3721 atcggtctcg gtgacgatgt cgaccgcgcc ttgcatgatg acatagg
//
LOCUS       DATCZV010000267         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_391932, whole genome shotgun sequence.
ACCESSION   DATCZV010000267 DATCZV010000000
VERSION     DATCZV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391932"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VIW27_05935"
     CDS             complement(<1..756)
                     /locus_tag="VIW27_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739245.1"
                     /translation="MTPSDLARELLRPFTDSVLLIAIGVFGAALWLSLVIMQSGPLLV
                     IFGLFIALLVLGTLFRYAVQVLECRAQGLRTPVVDIDAVSLFGNLWTLSPLLLVIAFV
                     WGALYLNGTGNPGAAIAVSVLLALLLPASTGVLAITHSPLESIKPSAIYRLIERCGAG
                     YAWIPIVIAALLLASAGMARAGAPLLPIVLLRTYVIILLFTLTGEVVRRSGVFGEVSV
                     GTPQAGSEPDYHHELTADRRKVADHAYGFISRGN"
     gene            complement(743..1003)
                     /gene="yidD"
                     /locus_tag="VIW27_05940"
     CDS             complement(743..1003)
                     /gene="yidD"
                     /locus_tag="VIW27_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008032613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY5739246.1"
                     /translation="MKTIGQVLALPLRGLVWLYRYAISPLIGANCRFQPTCSAYAEEA
                     LRRHGAFKGGWLALKRIGKCHPWGGSGYDPVPGVNGERDDAE"
     gene            complement(1003..1425)
                     /locus_tag="VIW27_05945"
     CDS             complement(1003..1425)
                     /locus_tag="VIW27_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY5739247.1"
                     /translation="MNEIKEAQQELIEEFQFFDDWMDRYQYIIDLGRRLPEFPAAAMT
                     DENKIRGCQSQVWFLADMRGDRLHFQAISDAAIVSGLIAVLLRIYSDRKPRDILDTPA
                     DFVAALGLEQHLSPTRSNGLASMLQAIRRFAEEALRAA"
     gene            complement(1422..>2315)
                     /locus_tag="VIW27_05950"
     CDS             complement(1422..>2315)
                     /locus_tag="VIW27_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEY5739248.1"
                     /translation="IAGARVEVAPYKRSAQDFVLWKPSTPELPGWDSPWGRGRPGWHI
                     ECSAMSKKHLGITIDIHAGGQDLVFPHHENELAQSSCAHDGAPFARYWVHNGFLSMDS
                     EKMSKSLGNVLLVRDLVDKFPGEVLRLALLGAHYRQPLDWNDETIESAKRMLDRFYGA
                     VRGIDIFAEERAAAEPPAAFVKALEDDINTPKALAEMFNVTRELNKTADAGERKSLAL
                     SLLAAGDVIGLVQEDPEAWFAGGGDDEWNAERIETMLKEREAARAGKDFARADAIRDE
                     LAAAGIQIEDGAEGTRWRRSG"
BASE COUNT          455 a          759 c          696 g          405 t
ORIGIN      
        1 gttaccgcgg ctgataaaac cgtaggcgtg gtcggcgacc ttcctccggt ctgcagtcag
       61 ttcgtgatga taatccggtt cggaacctgc ctgcggcgta cccacgctga cttcgccgaa
      121 caccccgctc ctgcggacga cttcgccggt caaggtaaac agcaggatta tgacgtaggt
      181 tctcaacagc acgatcggca ggagcggcgc gcccgcccgc gccatgcccg cgctcgcgag
      241 cagcagggcg gcaatgacaa tcggaatcca ggcgtatccg gcgccgcagc gctcgatcag
      301 tcggtagata gcagaaggct tgatactctc cagtggcgaa tgcgtgatcg cgaggacgcc
      361 cgtcgatgcg ggcagtagca atgccagcag gacagatacc gcgattgccg cgcccgggtt
      421 gccggttcca ttgagataaa gcgcgcccca gacaaaggcg atcaccagca acagcggaga
      481 gagcgtccac agattgccga acagcgacac ggcgtcgata tcgacgacgg gcgtccgaag
      541 cccctgcgcc cggcactcga gcacctggac cgcgtaacga aacagagtgc ctagtaccag
      601 cagcgcaatg aaaaggccga atatgaccag cagcggacca ctctgcatga tcacgagcga
      661 cagccagagc gcggcgccga atacgccgat ggcgatcagc agcacggaat cggtaaacgg
      721 ccgcagcaac tccctggcaa ggtcactcgg cgtcatcgcg ctccccgttt acgccgggca
      781 ccgggtcgta gcccgaaccg ccccaggggt ggcatttgcc gatccttttt agcgccagcc
      841 agccaccctt gaatgcaccg tggcggcgca atgcctcctc agcgtacgcg gaacaggttg
      901 gctgaaaacg gcagtttgcg ccgatcaacg gcgatatcgc ataccggtaa agccagacga
      961 gtccccgcaa gggcagcgcg agcacctgcc cgatggtttt catcaggcag cccgcagcgc
     1021 ctcttcggca aatcgccgga tcgcctgcag catcgaggcg agaccgttgc tcctcgtcgg
     1081 gctcaggtgc tgctccagcc cgagtgcggc gacgaagtcg gccggggtat cgaggatgtc
     1141 ccgcggcttg cggtcgctgt agatcctcag caataccgcg atcagaccgg agacgatcgc
     1201 cgcgtcgctg atcgcctgga aatgcaggcg gtctccccgc atgtcggcga ggaaccacac
     1261 ctgcgactgg cagccgcgaa tcttgttctc gtcggtcatg gccgccgccg gaaactcggg
     1321 cagacggcgg ccgagatcga tgatgtactg gtagcgatcc atccagtcgt cgaagaactg
     1381 gaattcctcg atcagttcct gctgtgcctc cttgatttcg ttcatccgct tcgccgccag
     1441 cgggtgcctt cggcgccatc ctcgatctgg ataccggcgg cagccagttc gtcgcggatc
     1501 gcatcggcgc gcgcaaaatc cttgccggca cgcgctgctt cacgttcctt caacatggtt
     1561 tcgatccttt cggcattcca ttcatcgtcg cccccgccgg cgaaccaggc ctcggggtcc
     1621 tcctggacca ggccgatgac gtcgcccgcc gcgagtaacg acaacgccag ggacttgcgt
     1681 tcaccggcat cggcggtctt gttcagttcg cgggtcacgt tgaacatctc ggccagcgcc
     1741 ttcggcgtat tgatatcgtc ttccagcgcc ttgacgaacg cagcaggcgg ttccgcagcc
     1801 gcgcgctcct cggcgaatat gtcgataccg cgaacggcgc catagaaacg gtcaagcatg
     1861 cgtttggcgg actcgatcgt ctcgtcgttc cagtcgagcg gttgccggta atgcgcgccc
     1921 agaagagcga gccgcagcac ctcgccagga aacttgtcga ccaggtcgcg aacgagcaaa
     1981 acattgccga gcgacttcga catcttctcg ctgtccatgc tcaggaagcc gttgtgcacc
     2041 cagtaacgcg caaacggcgc gccgtcgtgc gcgcagctgc tctgcgccag ttcgttttca
     2101 tgatgcggga aaaccaggtc ctgcccgccc gcatggatat caatagtgat gccgagatgt
     2161 tttttcgaca ttgccgagca ttcgatgtgc cagccgggcc ggccacgacc ccatggcgaa
     2221 tcccagccgg gcaactccgg cgtcgacggt ttccagagca cgaaatcctg cgctgagcgc
     2281 ttgtagggcg caacctcgac acgagcgccc gcaat
//
LOCUS       DATCZV010000268         4167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_709860, whole genome shotgun sequence.
ACCESSION   DATCZV010000268 DATCZV010000000
VERSION     DATCZV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709860"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VIW27_05955"
     CDS             complement(<1..393)
                     /locus_tag="VIW27_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5739249.1"
                     /translation="MLEKILIANRGEIALRILRACREMDIRTVAVHSTADRDLKHVRL
                     ADESVCVGPASSLESYLNIPAIICAAELTNADAIHPGYGFLSENAEFAEQVESSGFKF
                     IGPNAESIRTMGDKVAAIKAMKEAGVPTV"
     gene            complement(401..850)
                     /gene="accB"
                     /locus_tag="VIW27_05960"
     CDS             complement(401..850)
                     /gene="accB"
                     /locus_tag="VIW27_05960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016795057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY5739250.1"
                     /translation="MDIRKIKKLIELLEESGIAEIEIKEGEESVRISRQSSAPVATYA
                     PPPIGQIPVAAAAAPVPAAVVEEAPADNGHMVRSPMVGTFYSAASPTSEPFVSHGQQV
                     NVGDTLCIIEAMKMMNQIEADKSGKISSILVENGSPVEFDQVLFVIE"
     gene            complement(864..1313)
                     /gene="aroQ"
                     /locus_tag="VIW27_05965"
     CDS             complement(864..1313)
                     /gene="aroQ"
                     /locus_tag="VIW27_05965"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY5739251.1"
                     /translation="MAKLLVIHGPNLNLLGKREPEIYGADTLATIDEQLSQQAQRLGV
                     ELEVFQSNDEGTLVDRIQQAAAAGVDFVIINPAAFTHTSVAIRDVLLATRIPFIEVHL
                     SNIHARESWRKHSYLADIAVGVICGFGRTSYSLALEAAVVHCKQNAK"
     gene            complement(1454..1987)
                     /locus_tag="VIW27_05970"
     CDS             complement(1454..1987)
                     /locus_tag="VIW27_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5739252.1"
                     /translation="MNRDKLLLAAIGLASFVAGGVLYWLLQPDAPQTVANSPAPLELH
                     SIPLLDLDDAETRLADWQDELLIVNFWAPWCAPCRREIPVLIEVRQQYAARGVRLLGI
                     AFDNLEQVRRFAADYRIDYPLFLAGNRSAMYNAAFDNPTGTLPYTALLDGQLRIVYQH
                     HGELTAAELKAELEKHL"
     gene            complement(1984..>4167)
                     /gene="dsbD"
                     /locus_tag="VIW27_05975"
     CDS             complement(1984..>4167)
                     /gene="dsbD"
                     /locus_tag="VIW27_05975"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY5739253.1"
                     /translation="GEPRIPAGKVKQDEFFGRVETYRDQVRIELPLVYDGAPASRLQA
                     TAVSQGCADIGVCYPPLKQALDVDLTASGAIMPTAWKPSQQPAAASDDVVALQALLGE
                     VTDNLESNRQSREVAAASEADPLASLQALGAELGLGSDDEILHPDEAFVLTARLGADN
                     IIHTDISMPEHIYVYRDKVKIALAEGEGHSLGPISVPRGKRKVDEFFGPVEVIYGALP
                     VSIPIISADGASERFAISYSYQGCVEDRICYPPITKYLDVDAAAGLVQVVDQLSGAAP
                     GTATSAAQSTAPVSEQDRFAQLLLDESLLLVIGLFFLAGIGLTFTPCVFPMIPILSSI
                     IAGQGENITTGKAFTLSLVYVLSMAVTYAAAGAIVGYYGAEFNIQIWFQDPWILSIFA
                     AIFVLLALSMFGFYELQMPNAIQSRLTNLSNSQQGGTLIGVGLMGFFSAIIVGPCITA
                     PLVGALIFISQTQDWQLGGLALFALGLGMGVPLLLVGTSAGKLLPRAGGWMDRVKAVF
                     GVVLLGVAIWLLERILPVSITMALIAALLIGSGIYLGALDSLNESSSGWRRLAKSLGI
                     LILIYGIAYLVGTAAGSRDLLQPLRGITASVGGGPGAAGQHLAFRQIKGRDGLQLALN
                     DSAQQGRYTMLDFYADWCISCKEMEKYSFSHADVIASLDNVNLLQADVTKNDSLDVEL
                     MASLGIYGPPAILFFDTQGREIPGRRVVGFMDGEEFAAHVNAALQ"
BASE COUNT          782 a         1271 c         1349 g          765 t
ORIGIN      
        1 cacggtcggc acgccggcct ctttcatcgc cttgatcgcg gccaccttgt cgcccatggt
       61 gcgaatcgat tcggcattgg ggccgatgaa cttgaagccg ctcgactcta cctgctcggc
      121 gaattcggcg ttttccgaca gaaagccgta gccgggatga atcgcatcgg cattggtcaa
      181 ctcggcggcg cagatgatcg cgggtatgtt caggtaactt tccagcgacg acgccgggcc
      241 gacgcagacc gattcgtcgg ccaggcgcac gtgcttcagg tcgcggtcgg cggtcgaatg
      301 cacggcgacc gtgcgaatgt ccatttcccg gcaggcacgc aggatgcgca gtgcgatttc
      361 accacgattg gcgatcagaa ttttttccag catcgaacgg ctactcgatg acgaacagca
      421 cctggtcgaa ttcgaccggg ctgccgttct cgaccaggat cgagctgatt ttgccggact
      481 tgtcggcctc gatctggttc atcattttca ttgcctcgat gatgcacagc gtatcgccga
      541 cgttcacctg ctgtccgtgg gaaacgaacg gttccgaggt tggcgaggcc gccgagtaaa
      601 aggtgccgac catcggcgat ctgaccatgt ggccattgtc ggcgggcgct tcttcgacga
      661 ccgctgccgg aaccggtgcg gcagccgcgg cgacgggtat ctgtccgatt ggcggtggcg
      721 cataggtcgc caccggggcg gaggactggc ggctgatgcg caccgattcc tcgccttcct
      781 tgatttcgat ctcggcgata cccgactcct cgaggagttc gatgagtttc ttgatttttc
      841 gtatatccat ctggtcttaa tctctatttc gcgttttgtt tgcaatgtac aacggcggcc
      901 tcgagcgcca gcgagtagct ggtcctgcca aagccgcaga tcacgcctac ggcgatgtcc
      961 gcaaggtagg aatgcttgcg ccaggactcg cgggcatgga tattggacaa gtgaacctcg
     1021 atgaacggga tgcgcgtcgc cagcaacacg tcgcgtatcg cgacgctggt gtgggtgaac
     1081 gccgccgggt tgatgatcac gaagtcgacc cccgccgccg cggcctgctg aatacggtcg
     1141 accagcgtgc cttcgtcgtt gctctggaag acctcgagtt cgacgccgag tcgctgtgcc
     1201 tgttgcgaca gctgctcgtc gatggtcgcc agcgtatcgg ccccgtaaat ctcgggctcg
     1261 cgcttaccta gcaggttcag gttgggtccg tggatgacca gcagtttcgc cataatgaat
     1321 aggaatccgg agccggggtc ggttagttcg agattctcac atatcggtaa gatagagtcc
     1381 agttatagcc agatatgctc tgttttgccg aagttgtcga caaattttac tgaaaatcag
     1441 gcgccgcgac gcgctacagg tgcttttcca gctccgcctt tagctccgcg gccgtcaatt
     1501 ctccatggtg ctggtagacg atgcgcagct ggccgtcgag cagcgcggta tagggcaggg
     1561 tgccagtcgg gttgtcgaac gcggcgttgt acatcgcgct gcgattcccg gccaggaaca
     1621 gcggataatc gatccggtag tcggcggcga agcggcgcac ctgctcgagg ttgtcgaaag
     1681 cgatgccgag caggcgcacg ccgcgcgccg catactgctg ccgaacttcg atcaggaccg
     1741 gaatctcgcg ccggcacggc gcgcaccagg gtgcccagaa gttgactatc aacagttcgt
     1801 cctgccagtc ggcgaggcgc gtctcggcat cgtcgagatc tagcagcgga atcgagtgca
     1861 gttcgagggg agcgggcgaa ttcgcgacgg tttgcggcgc gtcgggttgc agcagccagt
     1921 agaggacgcc gcccgcgacg aacgaggcca gcccgatggc cgccagcagc agcttgtcgc
     1981 ggttcactgc aaggcggcgt tgacgtgggc ggcgaattcc tcgccgtcca tgaaaccgac
     2041 cacgcggcgg ccgggaattt cgcggccctg ggtatcgaaa aacaggattg ccggcgggcc
     2101 gtagataccg agcgaggcca tcaattcgac atcgagacta tcgttcttgg tcacgtccgc
     2161 ctgcagtaaa ttaacattgt ccagcgacgc gatgacatcg gcgtggctga acgagtactt
     2221 ttccatctcc ttgcacgaga tgcaccagtc ggcataaaag tcgagcatgg tgtagcggcc
     2281 ctgctgcgcc gagtcgttga gcgccagctg caggccgtcg cggcctttga tctggcggaa
     2341 cgcgaggtgc tgtccggccg caccgggccc gccgccgacc gaagcggtga tgcctctaag
     2401 cggttgcagc aggtcgcggc tgccggcggc agtaccgacc agataagcga tgccgtaaat
     2461 caggatcagg atgcccagcg acttggcaag ccggcgccag cccgacgagc tctcgttgag
     2521 cgaatccagc gcgccgagat agatgcccga accgatcagc agtgcggcga tcagcgccat
     2581 cgttatgctg accggcagga tgcgttccag cagccagata gcgacgccga gtaataccac
     2641 gccgaacacc gccttgaccc ggtccatcca gccgccggcg cgcggcagca gcttgccggc
     2701 cgaggtgccg accagcaaca gcggtacgcc catgccgagg cccaatgcga acagcgccag
     2761 cccgccgagc tgccagtcct gggtctggga aatgaagatc agcgcgccga ccagtggcgc
     2821 ggtgatgcac ggaccgacga tgatcgccga aaagaagccc atcaggccga cgccgatcag
     2881 cgtgccgccc tgctggctgt tactgagatt ggtcagccgc gactggatcg cgttcggcat
     2941 ctgcagctcg taaaagccga acatcgacag cgccagcaac acgaagatgg ccgcgaagat
     3001 cgacagaatc cacggatcct ggaaccagat ctggatattg aattcggcgc cgtagtaacc
     3061 gacgatcgcg cccgccgcgg cataggtgac cgccatcgac agcacgtaga ccagcgaaag
     3121 cgtgaatgcc ttgccggtgg tgatgttctc gccctgcccg gcgatgatgc tcgacaggat
     3181 cgggatcatc gggaatacgc agggtgtgaa ggtcaggccg ataccggcga gaaagaacaa
     3241 cccgatgacc aacagcaggc tttcgtcgag cagcagctgg gcgaagcggt cctgctccga
     3301 gaccggtgcc gtggactgcg ccgcggacgt cgcggtacca ggggcggcac ccgaaagctg
     3361 gtcgacgacc tgcaccagtc cggcggcggc gtcgacgtcg aggtacttgg tgatcggcgg
     3421 ataacagatg cggtcttcga cgcagccctg gtaggaatac gagatcgcga agcgctcgct
     3481 ggcgccgtcc gcggagatga tcggaatcga taccggcaga gcgccgtaga tgacctcgac
     3541 cggcccgaag aattcatcga ccttgcgctt gccgcgcggc accgaaatcg gccccagcga
     3601 gtgaccttcg ccctccgcca gcgcaatctt gaccttgtcg cgatacacgt agatatgctc
     3661 gggcatcgag atgtcggtat ggatgatatt gtccgcgccg agccgcgcgg tgagcacgaa
     3721 agcctcgtcc ggatggagga tttcgtcatc gctgccgagt cccagctcgg cgcccagcgc
     3781 ctgcaggctg gcgagcggat cggcttcgct ggcggcggcg acctcgcgcg actggcggtt
     3841 cgactccagg ttatcggtga cttcgccgag cagagcctgc agcgcgacaa cgtcgtcgct
     3901 ggctgccgcc ggctgttgcg agggcttcca ggcggtcggc atgatggcgc cgctggcggt
     3961 cagatcgacg tccagcgcct gcttgagcgg cgggtaacag acgccgatat cggcgcagcc
     4021 ctggctcacg gctgtggcct gcagccgcga ggccggcgcg ccgtcgtaga ccagtggcag
     4081 ctcgatgcgc acctggtcgc ggtaggtttc gaccctgccg aaaaactcgt cctgcttgac
     4141 cttgccggcc gggatccgcg gctcgcc
//
LOCUS       DATCZV010000269         3911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_180903, whole genome shotgun sequence.
ACCESSION   DATCZV010000269 DATCZV010000000
VERSION     DATCZV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180903"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="thiB"
                     /locus_tag="VIW27_05980"
     CDS             complement(<1..816)
                     /gene="thiB"
                     /locus_tag="VIW27_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020329519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HEY5739254.1"
                     /translation="MSRKLHALAGALAALLSFAAYGAGQTLTVYTYDSFTSDWGPGPK
                     VKQAFEARCDCVLELIGLEDGVSILNRLKLEGENTRADLLLGIDTNLTAEARATGLFD
                     KHGLELSASSLPGDWRDPHFLPYDYGYFAFVYNREKLADLPASLRALVDDSDGPTLLI
                     QDPRTSTPGLGLLLWMRKVFGDDAGEAWAKLAPRIVTVSKGWSEAYGLFLKGEADMVL
                     SYTTSPAYHLIAEQDASYAAARFTEGHYQQIEVAGMLAGSDNKALAQEFLRFMI"
     regulatory      complement(860..965)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1015..1482)
                     /locus_tag="VIW27_05985"
     CDS             complement(1015..1482)
                     /locus_tag="VIW27_05985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5739255.1"
                     /translation="MIIRKATYQDASELTEFNISMAHETEGLRLKPEVIGAGVRAMID
                     NPERGFYLVVELDNGIQASLMITTEWSDWRNGTFWWIQSVYVRPQYRRQGLYRELYAR
                     VKELAEQEPAVCGFRLYVERDNNIGQLTYRSQGMSKTDYYIFEELVPGLDFSD"
     gene            complement(1500..2408)
                     /gene="ylqF"
                     /locus_tag="VIW27_05990"
     CDS             complement(1500..2408)
                     /gene="ylqF"
                     /locus_tag="VIW27_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEY5739256.1"
                     /translation="MIHWYPGHMHKAGKEFKQILPQIDLVIEVLDARLPGSSENPLLA
                     ELRGDKPCLKLLNKSDLADDAILVDWIDALRQQSGVTTLACSIRDDSIGRVPGLCREM
                     IAQRDNRSPMVYALIAGIPNAGKSTLINRLAGRRVARTGNEAAMTRMQQRIEISPELT
                     LIDTPGMLWPRIDNEASGYRLAATGAIRETALDLQAVADFVADFLLEAYPERVIRRYD
                     IDPLPATGLELLEAIARRRGCLVSGGRVDLEKVSRLLLGELRDGELGGICLETPRGFA
                     VEMQQAAARLEQKQAARAARKKTRRN"
     gene            2486..2929
                     /locus_tag="VIW27_05995"
     CDS             2486..2929
                     /locus_tag="VIW27_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5739257.1"
                     /translation="MAEIRHRVGIKGSAEAIYRLLTTDEGLARWWTTDTSGAGEPGTI
                     IHFRFGEDGPRFEVVELIPHRLVRWRHHGDLPEAWMGTEVSFRLDAEEKQTFVTFCHA
                     NWRLADDFLAHCSTKWAIFLMSLKAAIETGRGRPFPDDVHIDFDE"
     gene            2931..3167
                     /locus_tag="VIW27_06000"
     CDS             2931..3167
                     /locus_tag="VIW27_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY5739258.1"
                     /translation="MSDPDTIITELESEFVELYKILKFEGLADSGGDAKQAIADGLVS
                     VNGEVETRKRKKIRPGDQIDFIDHHIDVVDKSGG"
     gene            3273..>3911
                     /locus_tag="VIW27_06005"
     CDS             3273..>3911
                     /locus_tag="VIW27_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007104354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEY5739259.1"
                     /translation="MSAPFAIATLYRFVRLDDFEALREPLLAFMHEQRICGTLLLAAE
                     GINGTVAGNPKAIDALLDYLRRDPRLRDLDCKLSYDDEAPFYRARVKLKREIVTLGVD
                     GIDPDQAGTYVEPGDWNRLISDPDVTVIDIRNDYESDIGSFEHALQPNTASFREFPAY
                     VSENLDPARQRRVAMFCTGGIRCEKATAFLRQQGFDEVYHLRGGILNYLERIP"
BASE COUNT          728 a         1226 c         1181 g          776 t
ORIGIN      
        1 gatcatgaaa cgcaaaaact cctgtgccag cgccttgtta tcgctgccgg cgagcatacc
       61 ggcaacctcg atctgctgat aatggccctc ggtaaagcgc gccgcggcat aactcgcatc
      121 ctgttcggcg atcaggtggt aggccggcga cgtggtatag ctaagcacca tgtcggcctc
      181 gcccttgagg aacaggccgt aggcctcgct ccagcccttg ctgacggtga cgatgcgcgg
      241 cgccagtttc gcccaggcct caccggcgtc atcgccgaaa accttgcgca tccatagcag
      301 cagcccgagt ccgggagtcg aggtgcgcgg gtcctggatc aacagcgtcg ggccgtcgct
      361 atcgtcgacc agcgcgcgca ggctggcggg caggtcagcg agtttttcac ggttgtagac
      421 gaacgcgaag tagccgtaat cgtagggcag gaaatgcgga tcccgccagt cgccgggcag
      481 gctcgacgcg ctaagctcga gaccgtgttt gtcaaacagc ccggtggcgc gcgcctcggc
      541 ggtcaggttg gtgtcgatgc ccagcagcag atcggcgcgg gtattttcgc cttccagttt
      601 gagccggttc aaaatcgaca cgccatcctc gagcccgatc agttcgagca cgcaatcgca
      661 acgcgcttcg aaggcctgtt tgaccttcgg ccccggcccc cagtcgctgg tgaaagagtc
      721 gtaggtgtag acggtgagcg tctgcccggc gccgtacgcg gcgaacgaca gcagcgcggc
      781 cagcgcgccg gccagagcat gcaatttacg agacatcgga ttctccattt gcccggattc
      841 aaaaacggag agatgggaga actgggattc gctcacatcc ctacgccagc attacctggt
      901 tcaggttcat cgagttggcg cgggttcgcg cctctcagct cgttcgagca ccccgttgag
      961 gagccacgac tataataccg gcgcccgggc atggcaagtc ggcggctgcg agcgtcagtc
     1021 ggagaaatcg agtccgggaa cgagttcttc gaagatgtag taatcggttt tcgacatgcc
     1081 ctgcgagcgg taggttaact gcccgatatt gttgtcgcgc tcgacataaa gccgaaagcc
     1141 gcagaccgcg ggttcctgct cggcgagctc ttttacgcgc gcgtagagtt ccctgtacaa
     1201 cccctggcgg cggtattgcg ggcgcacgta aacactctgg atccaccaga aggtgccgtt
     1261 gcgccaatcg ctccattcgg tggttatcat cagcgacgcc tgaatgccgt tatccagttc
     1321 gacgaccagg taaaaacctc tttccgggtt gtcgatcatc gcccgcacgc cggcgccgat
     1381 aacctcgggt ttcagccgca ggccctcggt ttcgtgcgcc atgctgatgt tgaattcggt
     1441 gagttcgctg gcatcctggt aagtcgcctt acgaataatc atgctgtttc tccctgcggc
     1501 tagttacgcc gggttttttt tcgcgccgcg cgcgcggctt gtttttgttc gagccttgcc
     1561 gcggcctgct gcatttcgac ggcgaagccg cgcggcgttt ccaggcagat accccccagt
     1621 tcgccatcac gcaactcgcc gagcaacagc cgcgaaactt tttccaggtc cacacggcct
     1681 ccactcacca gacaaccgcg ccggcgggcg atggcttcca gcaactcgag cccggttgcc
     1741 ggtaacgggt cgatatcata gcgccgaatc acgcgctcgg ggtaggcctc gagcaggaaa
     1801 tcggcgacga aatcggcgac cgcctgcagg tcgagcgcgg tttcgcgaat cgcaccggtg
     1861 gccgccagcc gatagccgct ggcctcgttg tcgattcgcg gccacagcat gccgggggta
     1921 tcgatcaacg tcagttcggg cgagatttcg atacgttgct gcatgcgcgt catcgccgcc
     1981 tcgttgccgg tgcgcgcgac ccggcgcccg gcgagcctgt tgatcagcgt cgacttgccg
     2041 gcgttgggga tgccggcgat caacgcgtag accatcggcg aacggttgtc gcgctgcgcg
     2101 atcatctcac ggcacaaccc gggcacgcga ccgatcgagt catcgcgaat gctgcaggcc
     2161 agggtggtga ccccgctctg ctgccgcaat gcgtcgatcc agtcgacgag gatcgcgtcg
     2221 tcggccaggt cgctcttgtt gagcaatttc aggcacggtt tgtcaccgcg cagctcggcg
     2281 agcagcgggt tctcgctgga cccgggcaga cgggcgtcga gcacctcgat gacgaggtcg
     2341 atctggggca ggatttgctt gaattccttg cccgccttgt gcatgtggcc ggggtaccag
     2401 tggatcatgt cgcgcctgtt atcgggaatt cggctattct cgccaaagct tttcccattg
     2461 tcattaaaac gtttaaacta gcaccatggc tgaaataagg caccgtgtcg gcatcaaagg
     2521 cagtgccgag gcgatctacc ggttattgac caccgacgaa ggcctcgccc gctggtggac
     2581 caccgatacc agcggggccg gcgagccggg tacgatcatc cactttcgtt tcggcgagga
     2641 cggccctcgt ttcgaagtcg tcgaactgat tccgcaccgg ctcgtgcgct ggcgccatca
     2701 cggcgatctg cccgaggcgt ggatggggac cgaggtcagc tttcgtctcg atgccgagga
     2761 aaaacagacc ttcgtcactt tctgccacgc caactggcgg cttgccgacg attttctcgc
     2821 ccactgcagc accaagtggg ctatcttctt gatgagcctg aaggcggcga tcgaaaccgg
     2881 ccgcggacga ccctttcccg acgacgtgca tatcgacttc gatgaatgac atgagtgatc
     2941 ccgataccat catcaccgaa ctcgagtcgg agttcgtcga gctttacaag atactcaagt
     3001 tcgagggcct ggccgatagc ggcggcgacg ccaagcaggc gatcgccgac ggcctggtca
     3061 gcgtcaacgg cgaagtcgaa acccgcaagc gcaagaaaat ccgccccggc gatcagatcg
     3121 atttcatcga tcatcatatc gacgtggtcg acaagtccgg gggctgaccg cggcgcgcgg
     3181 ccgcggcttg gctttgattt tctgccgcgc gcggctaaaa tacgcgtctt tttcaaaaac
     3241 ccggctctgg atttacgctc cagcaaggct cgatgtcagc accttttgcc attgccacac
     3301 tttaccgttt cgtgcgcctc gacgatttcg aggcgctgcg cgaaccgttg ctggctttca
     3361 tgcacgagca gcgaatctgc ggcaccctgt tactcgccgc cgagggcatc aacggcacgg
     3421 tcgccggtaa cccgaaggca atcgacgcgt tgctcgacta cctgcgccgc gatccgcggc
     3481 tgcgcgacct ggactgcaaa ctgtcgtacg acgacgaggc gccgttttac cgcgcgcgcg
     3541 tcaagttgaa gcgcgagatc gtcaccctcg gcgtcgacgg catcgatccc gaccaggccg
     3601 gcacttacgt cgagccgggc gactggaacc gcctgatcag cgatcccgat gtgaccgtca
     3661 tcgatatccg taacgattac gaatccgata tcggcagctt cgagcatgcg ctgcagccga
     3721 ataccgcctc gtttcgcgaa ttcccggcgt atgtcagcga aaatctcgac ccggcccgtc
     3781 agcgcagggt tgcgatgttt tgcaccgggg gcattcgctg cgaaaaggcc accgccttcc
     3841 tgcgccagca gggcttcgat gaggtttacc acctgcgcgg cggcatcctc aattacctcg
     3901 agcggatacc t
//
LOCUS       DATCZV010000270         5773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_657240, whole genome shotgun sequence.
ACCESSION   DATCZV010000270 DATCZV010000000
VERSION     DATCZV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657240"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VIW27_06010"
     CDS             complement(<1..528)
                     /locus_tag="VIW27_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY5739260.1"
                     /translation="MESSKKNTGILSRLNRRLAGTRDSEPEQAKLRLAIGFIVLVYIC
                     IPWGDTETFSDAITSLAGLIVILYYSGAIAIFLALIINPVQSQIRRFSGAALDLGSLS
                     ILMLHTGAEVVPLFLIYLWVILGNGFRFGLSNLYVSQIIAIIGFSAVVFWGEYWQQHR
                     SFGISLLLMLCVLPVY"
     gene            complement(550..1683)
                     /locus_tag="VIW27_06015"
     CDS             complement(550..1683)
                     /locus_tag="VIW27_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5739261.1"
                     /translation="MISSTENKTDIPKDSNIEPYENARRLSILRRQRRVLIVDDESTG
                     RTILAKIIQQAEEDVTVETFDNPVDALAWLDHNHPDLIITDYRMPDINGVELIKKIRA
                     KPACQDIPIMMITVVAEKSVRYDALEAGATAFLTRPIDQIECRTSCRNLLKLHEQQSI
                     IQDRADWLARQVEVATQQIVSREHETLLRLAKAGEYRDEVTGNHVVRMAKYSREIAEA
                     LGLSEKECDEIEYAAPMHDIGKIGIQDKILLKPGKFEQQEWLIMQQHTLIGHSILSNS
                     QSRYIQTGSIIALNHHERFDGSGYPNGIGGKEIPLVARIVAIADVYDALVSIRPYKKA
                     WDPRDAQDYLHKHAGSQFDPICVEAFFERIDNITNIHRAFSDN"
     gene            1989..2348
                     /locus_tag="VIW27_06020"
     CDS             1989..2348
                     /locus_tag="VIW27_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY5739262.1"
                     /translation="MSIDIHGAVYRVELATTPETRARGLMFRESLDPHGGMLLIFARE
                     GDRRIWMKNVPIALRVYWIDDDYEVVAELRLPPCKADPCPVYAAGRPARYVLELADRD
                     HPLRVGDRINGLDSLPR"
     gene            complement(2320..3507)
                     /locus_tag="VIW27_06025"
     CDS             complement(2320..3507)
                     /locus_tag="VIW27_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739263.1"
                     /translation="MNRSVLQLALAQAVMMSVNTLMISSAAIIGSQLAPSKAMATLPL
                     ALYFVAVMLTTIPASMLMGKLGRKPGFLLATVIGLGGGGCGVLGIYGESFVLFCLGSM
                     GVGVYTGFGNFFRFAAIETATGEKRNTALSYVLAGGILAAFIGPNLANFGRELFEITY
                     LGTLAAVIVLYGLNGINFLLMRLPRPTVHEIRASGRSMLQIAGQPGFIVAMMSAAIGY
                     SIMVLLMTASPLEMTHQNHAFSEVAFAIQWHVLGMFAPSFITGHLINRFGSQRVIIAG
                     VCLMVVCILLNFSGTTVTHFWTALLTLGVGWNFMFIGATSMLTETYRPAESARAQGIN
                     DFVVFTSAACSSFGAGALLHLLGWQLVNLAVVPLVLLTIAAHGWLWLKKRRQFSVAAS
                     QAH"
     gene            complement(3504..4394)
                     /locus_tag="VIW27_06030"
     CDS             complement(3504..4394)
                     /locus_tag="VIW27_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5739264.1"
                     /translation="MSQADLILRIVAIIFPVFSIVAVGYLYARFRHAADMSSGNRINM
                     DIFTPALIFDYMSGSDYALADYGYLSIGCLAVVLGSGLIAWPVARYLGYQWKTLVPPM
                     MFNNCGNMGLPLAALTFGEQMLPAAILLLAISNFLHFMVGQQLVTQQWDLRTVATNPM
                     IVATVLGITVSVAGITIPETLRMPIHMLGLVSIPLMLFSLGVRMVGIDFRDWGISTVG
                     ALLCPLSGLLIALPFVQLVPLPETQASLLILFSVLPPAVLNFMVAERYGQEPARVASI
                     VLIGNLSSVLVIPATLWFIL"
     gene            complement(4416..>5773)
                     /locus_tag="VIW27_06035"
     CDS             complement(4416..>5773)
                     /locus_tag="VIW27_06035"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5739265.1"
                     /translation="MRSSGSLSSGGAADSVAEGGADADADADAAADASGSAADPSANV
                     TAVWFGVMVGLNIQTSFLTPPFGFSLFYLRGVAPPNVKTTQIYKGAAAFIVLQLLGLA
                     IAGVFPNLVNYLPNKTHLISETAPPPNNPKLQECLEDYLFVQYDNQRQAIESGIEKIQ
                     TANLSYLPDEYQERLNESYRQAGEVFARVEAVRVAEENLEQYSVNYRPLHLEVRQIQR
                     EMRDLRDEVEELDQNRQRLRFEETVDEARIAEIERAIAVHEAGRKALEDSIPDEWKSA
                     RAEFNKFATAEKRARQQYRQSVDDSYAVIVMLREMLDQTDQLAALEDRFEPLLTAIES
                     RSAEAAMEEVEALEDDFNRLAETHLIVSKLSRARRALRALEPEVDKVRAEIGEAMRRF
                     SDELDWRQRAVTELASDLEGYDAVIARTIGMRMQERLTSEQADSVASCLAVHKDLTLY
                     F"
BASE COUNT         1203 a         1668 c         1721 g         1181 t
ORIGIN      
        1 atataccggc aggacacata gcattaacag caggctgatt ccgaagctac gatgctgctg
       61 ccagtactcg ccccagaata cgacggccga aaatccgatg atcgcgatta tctgggatac
      121 atacagattg gaaagcccga aacgaaatcc attgccgaga ataacccaga gataaatcag
      181 gaacagcggc acgacctcgg cgccggtatg cagcataaga atcgacaggg agccgaggtc
      241 cagtgcggcg ccggaaaaac gccgaatttg cgattgcact ggattgatga taagtgccag
      301 gaaaatcgca atcgcgccag agtaataaag gattacgatc aggccggcga gggaagtgat
      361 cgcatcggaa aaagtttctg tgtcgcccca gggaatacaa atataaacca ggacgataaa
      421 gcctatcgcc agtcgcagct tggcctgttc gggttcagag tctctcgtgc ccgccaatcg
      481 tcggttcaga cggctaagga taccggtatt tttcttggat gattccattt aacaccgaag
      541 tggctgcatt cagttatcgc taaaggctct gtgaatgttg gtgatattgt cgatgcgctc
      601 gaaaaaggcc tcaacgcaaa tcggatcgaa ctgggagccg gcatgcttgt gcaggtaatc
      661 ctgtgcatcc cgcggatccc aggctttctt gtacgggcgg atcgagacca gcgcgtcgta
      721 gacatcggcg atggcaacga tacgcgccac cagcggaatt tccttgccgc cgatgccgtt
      781 cgggtagccg ctgccgtcga agcgttcatg gtgattgagg gcaatgatcg agcccgtctg
      841 aatgtaacgc gactggctgt tcgacagaat cgagtgtccg atcagtgtgt gctgttgcat
      901 gatcagccat tcctgctgct cgaatttacc cggtttcagc aggatcttgt cctggatgcc
      961 gatcttgccg atatcgtgca tcggcgcggc atattcgatc tcgtcgcatt ccttttcgga
     1021 caggccgagt gcttcggcga tctcgcgcga atacttggcc atgcgcacca cgtggttgcc
     1081 ggtgacctcg tcgcgatatt cgccggcctt ggcgagccgc aacagggttt cgtgctcgcg
     1141 cgagacgatt tgctgggtcg cgacctcgac ctgacgggcc agccagtcgg cgcgatcctg
     1201 gatgatcgat tgctgctcgt gcagcttgag cagattgcgg caactggtgc ggcactcgat
     1261 ctgatcgatg ggccgggtca ggaatgccgt cgcgccggct tcgagggcgt cgtagcgcac
     1321 ggatttttcg gcgaccacgg taatcatcat gataggaata tcctggcaag ccggtttggc
     1381 gcgaattttc ttgatcaatt cgacgccgtt gatatcgggc atgcggtaat cggtaatgat
     1441 caggtcgggg tgattatgat ccagccaggc gagcgcgtcc accgggttgt caaaggtttc
     1501 gaccgtaaca tcctcttcgg cctgctggat gatcttcgcc aggatcgtgc gcccggtgct
     1561 ttcgtcgtcg acgatcagca cgcggcgctg acgccgcaga atgctaagtc tgcgagcgtt
     1621 ctcgtatggc tcgatattgg aatcctttgg tatatcggtt ttgttttctg tgctcgaaat
     1681 caattacaat acccctcatt gattctgaca gggccgggca ttttaacaaa atcaacaact
     1741 tatgtcgtac agttcaaaca gatatttgaa cacgacggta acgcggccct aagtcaattt
     1801 tgccgattaa atcctttata atcaaattat tatgtgacta agttaacaat acagattaaa
     1861 taagttaaat atctgcacct atgtcactct cggctttact tatagcacat tttcgcaaat
     1921 ggcaaatcgc gtcggtattt ttggtgttcg ctggtaacgc ggctgccgac tctcgaccgg
     1981 caaatctgtt gagcatcgat atccacggcg ctgtctatcg agtcgagctg gcgaccacgc
     2041 ccgagacccg cgccagaggg ctgatgttcc gcgaatctct cgatccgcac ggtggcatgc
     2101 tactgatctt cgcgcgcgaa ggcgatcgcc gcatctggat gaagaacgta ccgatcgcgt
     2161 tgcgcgtcta ctggatcgat gacgactacg aagtggtggc cgagctgcgc ctgccgccgt
     2221 gcaaggccga cccctgcccg gtgtacgcgg ccggcaggcc ggcgcgctac gttctcgaac
     2281 tggccgaccg cgaccacccg ctgcgggtcg gcgaccggat caatgggctt gactcgctgc
     2341 cacgctgaac tggcggcgct ttttcaacca cagccagccg tgcgcggcaa tcgtcagcaa
     2401 taccagcggt accaccgcca ggttgaccag ctgccagccg agcaggtgta gcagggcgcc
     2461 ggcgccgaag gacgaacagg cggcgctggt aaagacgacg aaatcgttga ttccctgcgc
     2521 cctggcgctt tccgccggcc tgtaggtttc ggtcagcatg ctggtggcgc cgataaacat
     2581 gaaattccag ccgaccccga gcgtcagcag cgcggtccag aaatgggtta ccgtggtgcc
     2641 gctgaagttc agcagtatgc aaaccaccat caggcacacc ccggcgatga tcacgcgttg
     2701 cgaaccgaat cgattgatca ggtggccggt gatgaaggac ggcgcgaaca tgccgaggac
     2761 atgccattgg attgcaaacg cgacctcgct gaaggcatgg ttttgatgag tcatttcgag
     2821 cgggctcgca gtcatcaaca gcaccatgat gctgtagccg atcgcggcgc tcatcatcgc
     2881 cacgatgaag ccgggctgcc cggctatttg cagcatcgac ctgccgctgg cgcgtatttc
     2941 gtgcaccgtc ggccgtggca gccgcatgag caggaagttg atgccgttga gcccatagag
     3001 gacgatgacc gcggccagcg tgcccaggta ggtgatttcg aacagttccc tgccgaaatt
     3061 cgccaggttg ggacctatga aagcggccag gattccgccc gccagcacgt agctgagcgc
     3121 ggtattcctt ttttcacccg tcgctgtctc gatcgcggca aagcgaaaga agttgccgaa
     3181 gccggtataa acgccaaccc ccatgctacc cagacaaaac agcacaaacg attcgccgta
     3241 aataccgagc acgccgcaac caccgccgcc gaggccgata accgttgcca gcaggaaacc
     3301 cggtttgcgg ccgagctttc ccatcagcat cgacgccggg atcgtggtca gcatgacagc
     3361 cacgaagtaa agcgccagcg gcagggtcgc catcgccttg ctcggtgcca gctggctgcc
     3421 gatgatcgcc gccgaagaga tcatcagcgt attcaccgac atcatcacgg cctgcgccag
     3481 tgccagctgc aagacgctgc gattcacagg ataaaccaca gcgttgccgg aataaccagc
     3541 acgctggaca ggttgccgat cagcacgatc gaggcgacac gcgcgggttc ctggccgtag
     3601 cgttcggcaa ccatgaagtt gagcacggcc ggcggcagca cgctgaacag gatcagcaaa
     3661 gatgcctggg tttcgggcag gggcaccagt tgcacgaacg gcagcgcgat cagcaggcca
     3721 ctgagtgggc agagcagggc gcccacggtg ctgatgcccc agtcgcgaaa gtcgatgccg
     3781 accatgcgca cgccgagtga gaacagcatc aatggaatcg ataccaggcc gagcatgtga
     3841 atcggcatgc gcagggtttc cggaatcgtt attcccgcta ccgatacggt gataccgaga
     3901 accgtggcga cgatcatcgg gttggtggcc acggtgcgca ggtcccattg ctgggtgacc
     3961 agttgctgac cgaccatgaa gtgcaggaaa ttcgatatcg ccagcagcag gattgccgcc
     4021 ggcagcatct gttcaccaaa agtcagtgcc gccagcggca atcccatgtt gccgcagttg
     4081 ttgaacatca tcggcggtac cagcgttttc cactggtagc cgagatagcg cgccaccggc
     4141 caggcaatca atcccgaccc cagcaccacg gcgagacagc cgatcgacag gtaaccgtag
     4201 tcggcgagcg cataatcgct gccggacatg taatcgaaga tcagcgccgg ggtgaatatg
     4261 tccatgttga tgcggttgcc cgatgacatg tcggccgcat gacgaaatcg cgcgtacagg
     4321 taaccgacgg cgacgatgct gaacaccgga aagatgatcg caacgatgcg cagaatcaga
     4381 tctgcctgtg acattcggtt actcgaagtg gagttctaga agtagagggt caggtccttg
     4441 tgtaccgcga ggcaactggc caccgaatcg gcctgctccg aggtcaggcg ctcctgcata
     4501 cgcataccga tagtcctggc gatcaccgcg tcataccctt cgaggtcgct ggccagttcg
     4561 gttactgccc gctgccgcca atcgagttcg tcgctgaagc gtcgcatggc ctcgccgatt
     4621 tccgcgcgca ccttgtcgac ttccggctcc agtgcccgca aggctcgccg ggcgcgtgat
     4681 agcttgctga cgataaggtg ggtctccgca agccggttga aatcgtcctc gagcgcctcg
     4741 acctcttcca tggcggcttc cgcggatcgg ctttcgatcg ccgtcagcag gggttcgaag
     4801 cgatcctcga gcgccgccaa ctggtcggtc tgatcgagca tctcgcgcag catgacgatg
     4861 accgcgtaac tgtcgtcgac gctttgccgg tattgctgcc gcgcgcgttt ctcggcggtg
     4921 gcgaacttgt tgaactcggc gcgggcagat ttccactcat cgggaatgct gtcttccagc
     4981 gccttgcgcc cggcttcgtg gactgcgata gctctctcga tttcggcgat cctggcctcg
     5041 tcgaccgttt cctcgaatcg caggcgctgg cggttctggt cgagctcctc gacttcgtcg
     5101 cgaagatcgc gcatctcgcg ctggatctgg cggacctcga gatgcagcgg ccggtagttc
     5161 accgaatatt gttcgagatt ttcctcggcg acgcgaaccg cttcgacccg tgcgaagact
     5221 tcaccggcct ggcgatagct ttcattgagg cgttcctggt actcgtccgg aagataggag
     5281 agattggccg tctggatttt ctcgatgccg ctttcgatcg cctggcgctg gttgtcgtac
     5341 tgcacgaaca ggtaatcctc gagacattcc tgcagtttcg gattgttcgg cggcggcgcg
     5401 gtttccgata tcaggtgggt cttgttgggc agataattga ccaggttcgg gaacacgccg
     5461 gcgatcgcca gaccgagcag ctgcaggacg atgaaagccg ccgctccctt gtagatttgc
     5521 gtggtcttga cgttcggcgg cgcgacaccg cgcaggtaga acagcgagaa accgaacggc
     5581 ggtgtcagga acgaagtctg gatattgaga ccgaccatga ccccgaacca gaccgcggta
     5641 acgttggcgc tcgggtcggc ggcgctgccg ctggcgtcgg cggcggcgtc ggcgtcggcg
     5701 tcggcgtcgg cgccaccctc ggccaccgag tcggcggcgc caccgctcga cagcgagccc
     5761 gacgagcgca tct
//
LOCUS       DATCZV010000271         7684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_551942, whole genome shotgun sequence.
ACCESSION   DATCZV010000271 DATCZV010000000
VERSION     DATCZV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551942"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VIW27_06040"
     CDS             complement(<1..593)
                     /locus_tag="VIW27_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5739266.1"
                     /translation="MARLRRYCPAGIPVHVIQRGNNRQDCFRESQDKAAYHQYLQKAS
                     NLHALDVHAWVFMSNHVHLLVTPENASSVSKAMQSLGRQYVHYFNKKYSRTGTLWEGR
                     FKSCLVQTEKYFMICQRYIELNPVRAGLAPHPLAFHWSSYKANALGVESELVTPHEVF
                     LSLGRSATDRQSAYRALFGQVIQPAQLELIRETVNSGL"
     gene            complement(765..1418)
                     /locus_tag="VIW27_06045"
     CDS             complement(765..1418)
                     /locus_tag="VIW27_06045"
                     /inference="COORDINATES: protein motif:HMM:NF023939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3726 domain-containing protein"
                     /protein_id="HEY5739267.1"
                     /translation="MTYRLTLSETETSLRKAARAVGLDWGLAEEAGKAARWLAAFGLP
                     GPAIMFAHLERLRGADYNEFVPDCGREPWQAAGGLLCPVITGAALADRSAQLVAGKQF
                     ELGRTAYPLLLVAILGQAARHHDVAFATAWAGVQVDCFGDGLCIQGAHDDLLCAETDA
                     VHCRRNDSAVAEMRPSTRACEIDAAIFRRIDALAFETYVPASEASRAGAGAGLTDND"
     gene            complement(1415..3088)
                     /locus_tag="VIW27_06050"
     CDS             complement(1415..3088)
                     /locus_tag="VIW27_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008281947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739268.1"
                     /translation="MTCNQHNPFPEAAAFRRPPEVVMRLARMGSFHQTRLSFMRALLR
                     RLKRESWTFRRNLWVLDDNGFGRAVYTVYGPERAYSLVAFSHYLADEMRSDRVIATAW
                     DATFALFDGVPDEDDLDRLEQNVPKQEAGRISDSELSLSRANKSVRLWKHVVDSLAAG
                     EQPDREQLEAVGYLVRTTAVYGSGKFGAADRLAIADRPELSGPFQAELLSVWLIRAFS
                     IDLVEHVAAARGGAQAVTIEPALRRRIGVGNSTGLGMAPFLVNHPALLNNWMMAREEA
                     LARVRALPRAEPTAIAEFRRHFELARDNAAQWHSEHPIQVEKLRALRADLERMADYLA
                     SESLGDDHPWDRFYRWAEERLSLEGQEQLVSLLLEPYGELVDELSQCMDADEHKSFFI
                     DGSMPVAGLLEILDTRCRWALDVDYGQADNCARFWYTSAEKLEPRVGERFDEPGAEYE
                     LPLAIGRDLQALHAQLQDWPADASVAEFLLAHPEHRHSARRVQNLVALPYAEIQDNLI
                     AADMLPIDILRCKLSFFGATRFDPRSDRWVRICMYQDAPFPHELLAAAP"
     gene            complement(3085..4077)
                     /locus_tag="VIW27_06055"
     CDS             complement(3085..4077)
                     /locus_tag="VIW27_06055"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY5739269.1"
                     /translation="MSETAFRIDALQYCNWSREIFEQMRAGGVDAVHVTIAYHENFRE
                     TVANMEQWNACFERYPELIFQGFTGDDVRRARETGRTAIFYGFQNPSPIEDDIRLVEI
                     LHRLGARFMQLSYNNQSLLATGCYEAEDPGITRMGREVIAEMNRVGLVVDMSHSAERS
                     TLEAIEISSRPIVISHANPAFWHPALRNKSNTVLRALGDSGGMLGFSLYPHHLQDGSN
                     CSLESFCRMVGECAELMGVEHIGIGSDLCQDQPDSVVEWMRVGRWTKKIDYGEGSASN
                     AGFPPMPSWFGGNRDFGKVEQGLRDAGFEARDVAAIMGNNWLRFFDENFAPLNQ"
     gene            complement(4074..5525)
                     /locus_tag="VIW27_06060"
     CDS             complement(4074..5525)
                     /locus_tag="VIW27_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5739270.1"
                     /translation="MTEVTQNYIGGEWLAGDSEIANINPSDLSDTIGRYAQASGEQLE
                     RALDAAEQAQREWAATGLEARYNALMAIGNELMARAEELGKLLSREEGKPLAEGKGEV
                     FRSGQFFTYFAAEVLRQVGDNADSVRDGIEIDVRREPVGTVAIISPWNFPMATAVWKI
                     APALAFGNAVIWKPANLVPASAVALAEIISRQNLPAGLFNLVMGSGSGIGQQLVESPR
                     VDAISFTGSVPVGRGIAQAAVGNFTKLQLEMGSKNALAVMDDADLDLALACAMNGAFG
                     GSGQKCTASSRLVVHQRVHDYFVERLAEAAESMVVGHALEPATQMGPVVSAEQLEQNL
                     ENIELAKQEGGELLCGGERVERDTEGYFMTPAVIADTRNDWQVNREELFAPIACVIKV
                     DSYDEALATVNDTRFGLTAGICTTSLSRATHFRRHARTGCVMVNLPTAGTDYHVPFGG
                     RGESSYGPREQGQYAREFYTAVKTAYIASGNPA"
     gene            5622..6506
                     /locus_tag="VIW27_06065"
     CDS             5622..6506
                     /locus_tag="VIW27_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5739271.1"
                     /translation="MNIKLEQFRVFVAVAESGSITAAARRIGRSMAAVSMTLSRIEHQ
                     IGTPLFDGERKSRLTPLGRHTLQLARRAIDEHDNALRAIARFASGAEGMSRIAVVPSV
                     ATRLLPAAVGELTRANPDTQIHIRDLDSAAIHEALRAGTVDFGVAGRPESGGLEAEFL
                     LADPFRLVCRRDHRLAGLARPLDWRDLDGETLIVNGLCQSIDDAGWQGLIAAATLYIH
                     NTWSILSFVEDGIGVTLLPALAQPISADLVSLPLARASLRRELYILRREDSSLSPLDL
                     RLIDAIHRRAAALEFTAA"
     gene            complement(6589..7608)
                     /locus_tag="VIW27_06070"
     CDS             complement(6589..7608)
                     /locus_tag="VIW27_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5739272.1"
                     /translation="MATALCSLLSGCGVNTGVVFDPNKVHHGDGEFVSVKQGSFFGHF
                     MMRLREDDPPPPDPKQVSSIVTAADLELIASPADVPRVTWIGHATALVQYRGINYLTD
                     PHLTQYPFAFEFWVEPRFTQPALTFEQLPPIDFIVISHNHFDSLDHGTVEMFGDSVLW
                     YVPLGLKAWFIDRGISPARVVELDWWDRHNYDDRVEVTLTPAQHWSRRSVWDTNKTLW
                     GGWAVKIDDFNSYFAGDTGYNERYFREIGARLGPFRLAMIPIGAYAPRYFMGSAHADP
                     AQAVDIHLEVGAQQSLPIHWGTFQLTIEPILEPAQLLRAETAARGLPAEQFRPVQIGE
                     TLLLP"
BASE COUNT         1449 a         2455 c         2383 g         1397 t
ORIGIN      
        1 agtccactgt tgacggtttc gcgaatcaat tcgagctgag ccggttggat gacctgtccg
       61 aacaaggccc gatatgccga ctgcctgtcc gtggccgaac gcccaagtga caggaagacc
      121 tcatgaggcg tcaccagttc agactcgact ccgagcgcgt ttgccttgta gctcgaccag
      181 tgaaacgcca gcggatgcgg ggcaagtcca gcacggaccg ggttcaattc gatgtaacgt
      241 tggcagatca tgaaatattt ttcagtctgc accagacatg atttgaaccg gccctcccac
      301 agcgtgccgg ttctcgaata cttcttgttg aagtaatgaa catattgcct gcccaacgat
      361 tgcatcgctt tagaaaccga gctggcattc tctggtgtca ccagcaagtg aacatgattc
      421 gacatgaata cccaggcatg tacgtcgagc gcgtgcaggt tcgacgcttt ttgcagatat
      481 tggtgatacg cggctttatc ctgggattcg cgaaagcagt cctggcgatt attgcctcgc
      541 tgaatcacgt ggaccggaat tcctgccggg cagtatcttc ttaaacgtgc catggtgttc
      601 ctccttgaac gactgaccac gctgcgattg taacctaaaa agttgcgttg atccctgcat
      661 atgccgtcgc cagaacgagc aaaaactcag gttgcgtttg ctggaggaca taaattgact
      721 ctgaccccaa tttagcttgg ggggaaaatg actctgaccc cattttagtc gttgtcggtg
      781 aggccggcgc cggcgccggc gcgtgaggct tcgctggccg gcacgtaggt ttcaaaggcg
      841 agcgcgtcga tgcgcctgaa gatcgctgcg tcgatttcgc aggcgcgcgt cgagggccgc
      901 atttcggcga ccgccgaatc gttgcgccga cagtgaaccg cgtcagtttc ggcgcacaac
      961 aggtcgtcgt gcgcaccctg gatgcacagg ccgtcaccaa aacagtcgac ctgcaccccc
     1021 gcccacgccg tcgcgaaagc gacgtcgtga tggcgcgcgg cctggccgag gatcgcgacc
     1081 agcaacaacg ggtaagccgt ccggccaagc tcgaattgtt tgccggcaac cagctgtgcc
     1141 gagcgatcgg cgagcgcggc accggtgatt accgggcaca acagaccgcc cgcggcctgc
     1201 cagggttcac ggccgcaatc ggggacgaat tcgttgtaat ccgcgccgcg cagtcgctcg
     1261 aggtgcgcga acatgattgc gggccccggc aaaccgaaag ccgccagcca gcgggccgcc
     1321 ttgccggcct cctcggcaag cccccaatcg agaccgacgg ctcgcgccgc cttgcgcagc
     1381 gaggtttcgg tctcggacag ggtcagccgg taggtcacgg cgcagcggcg agcagttcgt
     1441 gcgggaacgg cgcatcctgg tacatgcaga tgcgcaccca gcggtcggag cgcggatcga
     1501 agcgcgtcgc accgaagaac gacagcttgc agcgcaatat gtcgatcggc agcatgtcgg
     1561 cggcgatcag gttatcctgg atctcggcat agggcagcgc gaccaggttt tgcacgcggc
     1621 gcgcgctatg gcggtgctcg ggatgcgcga gcagaaattc ggcgacgctg gcgtctgccg
     1681 gccaatcctg caattgcgca tgcaacgcct gcagatcgcg gccgatggcg agcggcaact
     1741 cgtattccgc gccgggctcg tcgaagcgtt cgccgacgcg cggctcgagc ttctcggccg
     1801 acgtgtacca gaagcgcgcg cagttgtcgg cttgcccgta gtccacgtcc agcgcccacc
     1861 ggcaacgcgt gtcgagaatt tcgagcaacc cggcgaccgg catcgagccg tcgatgaaaa
     1921 agcttttgtg ttcgtcggca tccatgcact gcgacaactc gtcgaccagt tcgccgtaag
     1981 gctcgagcag cagggacacc agttgttcct ggccttcgag cgagaggcgt tcctcggccc
     2041 agcgataaaa ccgatcccac gggtgatcat caccgagcga ctcgcttgcc aggtaatccg
     2101 ccatacgttc gagatcggcg cgcagcgcac gcaatttctc gacctggatc ggatgctccg
     2161 aatgccattg cgcggcattg tcgcgcgcca gctcgaaatg ccgccggaat tcggcgatcg
     2221 cggtcggctc ggcgcgcggc agcgcgcgca cccgcgcgag cgcttcctcg cgcgccatca
     2281 tccagttatt cagcagcgcc gggtgattga ccaggaacgg cgccatgccg agcccggtgg
     2341 aattgccgac gccaatacgc cgccgcagcg cgggttcgat agtgactgcc tgtgcgccac
     2401 cgcgtgccgc cgcgacatgc tcgaccaggt cgatcgagaa cgcgcgaatc agccacaccg
     2461 acagcagttc ggcctgaaac gggccgctga gttccgggcg atcggcgatc gccagacgat
     2521 ccgcggcgcc gaacttgccg gagccgtaaa ccgccgtggt gcgcaccaga tagccaaccg
     2581 cttcgagttg ttcgcggtcg ggctgttcgc cggcagcaag gctgtcgacc acgtgcttcc
     2641 acaggcgtac cgacttgttg gcgcgcgaca gcgacaactc cgagtccgag atgcggccgg
     2701 cttcctgctt cggtacgttt tgctcgaggc gatcaagatc atcctcgtcg gggacaccgt
     2761 cgaacagcgc aaaggtcgcg tcccaggcgg tcgcgataac gcggtcggaa cgcatttcgt
     2821 cggcgaggta atgcgaaaaa gcgaccagcg agtaagcacg ctcgggaccg taaaccgtgt
     2881 acacggcgcg gccgaagcca ttgtcgtcga gcacccacag gttgcgcctg aaggtccagc
     2941 tttcgcgctt cagacggcgc aacagcgcgc gcatgaacga cagccgcgtc tggtgaaaac
     3001 tgcccatgcg cgcaagccgc atgaccacct cgggcgggcg acggaacgcg gcagcctcgg
     3061 gaaacgggtt gtgctggttg caggtcattg attcaacggc gcaaaatttt cgtcgaagaa
     3121 ccgcagccag ttattgccca tgatcgcggc gacatcgcgc gcctcgaaac cggcatcacg
     3181 cagcccctgc tcgaccttgc caaaatcgcg gttgccgccg aaccaggatg gcatcggcgg
     3241 aaagcccgcg ttactcgccg aaccctcgcc ataatcgatt ttcttcgtcc agcggccgac
     3301 gcgcatccac tcgaccaccg agtcgggctg atcctgacac aggtcggagc cgatgccgat
     3361 atgctcgacg cccatcagtt cggcgcattc accgaccatg cggcaaaaac tctccaggct
     3421 gcagttgctg ccgtcctgca aatgatgtgg gtaaaggctg aagccgagca tgccgccact
     3481 gtcgccaagc gccctaagca ccgtgttcga cttgttgcgc agcgccgggt gccagaaggc
     3541 cgggttggca tggctgatca cgatcgggcg gctgctgatc tcgatcgcct ccagcgtcga
     3601 acgctcggcc gaatggctca tgtcgaccac caggccgaca cgattcatct cggcgatgac
     3661 ttcacgcccc atgcgcgtga tgcccggatc ctcggcctcg tagcagccgg tggcaagcag
     3721 cgactggttg ttgtaggaca gctgcatgaa acgcgcgccg agccggtgca ggatttcgac
     3781 caggcgaata tcgtcctcga tcggcgatgg attctgaaag ccgtaaaaga tcgcggtgcg
     3841 gccggtctcg cgcgcccgcc gcacgtcgtc gccggtgaat ccctgaaaga tcaactccgg
     3901 gtagcgttcg aagcacgcgt tccattgctc catgttggcc acggtttcgc gaaagttttc
     3961 gtggtaggcg atggtgacgt gcaccgcgtc gacgccgccg gcgcgcatct gctcgaagat
     4021 ttcccgcgac cagttgcagt actgcagcgc gtctatgcgg aaagcggtct cactcatgcc
     4081 ggattgccgc tggcgatata ggcggttttc accgcggtgt aaaactcgcg cgcgtactgg
     4141 ccctgctcgc gcggtccata cgacgattcg ccgcggccgc caaacggcac gtggtaatcg
     4201 gtgccggcgg tcggcaggtt gaccatcacg cagccggtgc gcgcgtggcg gcgaaaatgg
     4261 gtggcgcgcg acaggctggt agtgcagatg ccggcggtga gcccgaagcg cgtatcgttg
     4321 accgtcgcca gtgcctcgtc gtaggaatcg accttgatta cgcaggcgat cggcgcgaac
     4381 aattcctcgc ggttgacctg ccaatcgttg cgggtgtcgg cgatcaccgc cggcgtcatg
     4441 aagtaaccct cggtgtcgcg ctcgacgcgc tcgccgccac acagcagctc gccgccttcc
     4501 tgtttggcga gctcgatatt ttccaggttc tgttccagct gttcagcgct gaccaccggt
     4561 cccatctgtg tcgccggttc gagcgcatgg ccgaccacca tcgactcggc ggcctccgcc
     4621 aggcgctcga cgaagtaatc gtgcacgcgc tggtgcacga cgaggcgcga cgaggcggtg
     4681 cacttctgcc cgctgccgcc gaaagcaccg ttcatggcac aggcgagggc gagatcgaga
     4741 tcggcgtcgt ccatcaccgc gagcgcgttc ttggagccca tctcgagctg gagcttggta
     4801 aagttgccga ccgccgcctg cgcgatgccg cgccccaccg gcaccgagcc ggtgaagctg
     4861 atcgcgtcga cccgcggact ttcgaccagc tgctggccga taccgcttcc ggagcccatc
     4921 accaggttga agagcccggc cggtagattc tgtcgcgaga tgatctcggc cagcgccacc
     4981 gcgctcgccg gcaccagatt ggcgggcttc cagatcaccg cgttgccaaa agcgagcgcc
     5041 ggggcgattt tccacaccgc ggtcgccatc gggaagttcc acggcgagat gatcgccacc
     5101 gtgccgaccg gctcgcggcg cacgtcgatc tcgatgccgt cgcgcaccga gtcggcattg
     5161 tcaccgacct gacgcagcac ctcggccgcg aaataggtga aaaattgccc ggaacggaac
     5221 acttctccct tgccctcggc gagcggtttg ccctcttcgc gcgacaacag cttaccgagc
     5281 tcctcggcgc gcgccatcaa ctcgttaccg atcgccatca gcgcgttata gcgcgcctcg
     5341 aggccggtag ccgcccactc gcgctgagcc tgttcggccg cgtcgagcgc gcgttcgagt
     5401 tgctcaccac tcgcctgcgc atagcggccg atggtatcgg acaggtccga cgggttgata
     5461 ttggcgattt cgctgtcgcc ggcgagccat tcgccgccga tgtaattctg cgtgacttcg
     5521 gtcattgggg ttaccggatt cgggcaattc gatggacggt cacgatagtt ccgcgtaaag
     5581 tttcagtcaa tctgaaattt atgaattaga tttaagtttt attgaatatt aagctcgaac
     5641 aattccgcgt cttcgtcgcg gttgccgaga gcgggtcgat caccgcggcc gcgcgccgaa
     5701 tcggccgcag catggcggcg gtatcgatga ccctgagccg aatcgagcac cagatcggca
     5761 ctccgctatt cgacggcgag cgcaagtcgc ggctgacccc actcggccgc cacacactgc
     5821 agctggcacg gcgcgcgatc gacgaacacg acaacgcgct gcgcgcgatc gcacgcttcg
     5881 ccagcggcgc cgaaggcatg agccgcatcg cggtagtgcc gtcggtggcg acgcgactgc
     5941 tgccggccgc ggtcggcgaa ttgacgcgcg ctaatcccga cacgcagatt cacattcgcg
     6001 atctcgacag cgccgcgatc catgaggcgc tgcgcgcggg cacggtcgat ttcggcgtcg
     6061 ccggcaggcc cgagagtggc ggtctcgaag ccgaattcct gctcgccgac ccgtttcgcc
     6121 tggtttgccg ccgggatcac cgcctggccg gcctcgcacg gccactcgac tggcgcgacc
     6181 tcgacggcga aaccctgatc gtcaacgggc tgtgtcagtc catcgacgac gccggctggc
     6241 agggtttgat cgcggcggca acgctgtaca ttcataacac ctggtcgatc ctgtcgttcg
     6301 tcgaggacgg catcggcgta accctgctgc cggcactggc gcagccgatc agcgccgatc
     6361 tggtctcgct gccgctggcg cgggccagcc tgcgccgcga gctttatatc ctgaggcgcg
     6421 aggacagcag cctgagcccg ctcgacctac gcctgatcga cgccattcac cgccgggctg
     6481 ccgcgctcga attcaccgcc gcttaattca gatccgaccc aaattactcc gcgtcgataa
     6541 tttgggtcag atctgaatta agtgggcgct gatcattcgc gtacgggttc aggggagcag
     6601 cagggtctcg ccgatttgca cggggcgaaa ctgctccgca ggcaacccgc gcgcagcggt
     6661 ttcagcacgc agcaattgcg cgggttcgag gatcggttcg atggtcagct ggaaggtacc
     6721 ccaatggatc ggcagcgatt gctgcgcgcc gacctcgaga tgaatatcga ccgcctgcgc
     6781 cggatcggcg tgagcgctgc ccatgaagta acgcggggca taggcgccaa tcgggatcat
     6841 cgccagtcga aaaggcccga gccgcgcacc gatctcgcga aaatagcgct cgttgtaacc
     6901 ggtgtctccc gcgaagtagc tgttgaagtc gtcgatcttc accgcccagc cgccccacag
     6961 ggtcttgttg gtatcccaga ccgagcgccg cgaccagtgc tgtgccggcg tcagcgtgac
     7021 ctcgacgcgg tcgtcgtaat tgtgtctgtc ccaccagtcg agttcgacca cgcgcgcggg
     7081 cgagatgccg cgatcaataa accaggcctt gagcccgagc ggcacgtacc acagcaccga
     7141 gtcgccgaac atttccaccg taccgtggtc gagcgaatcg aaatggttgt gcgagatgac
     7201 gatgaaatcg atcggcggca gctgctcgaa ggtcagcgcg ggctgagtaa agcgcggctc
     7261 gacccagaac tcgaaagcga acgggtattg cgtcagatgc ggatcggtca ggtaattgat
     7321 gccacggtat tgcaccagcg ccgtcgcatg tccgatccag gtcacgcgcg gcacgtcggc
     7381 aggcgacgcg atcagctcga gatcggctgc ggtgacaatc gatgaaacct gcttcggatc
     7441 gggcggtggc ggatcatcct cgcgcaggcg catcatgaag tggccgaaga acgagccctg
     7501 cttgacgctg acgaattcac cgtcaccgtg gtgcaccttg ttcgggtcga agaccacgcc
     7561 ggtgttgaca ccacagcctg ataacaggct gcacagagca gttgccatga accaatgcca
     7621 acgctttcgc atcaatcgat gccgttgatc gccaacaagc cctgttctct gcataacctt
     7681 cgga
//
LOCUS       DATCZV010000272         6663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_286246, whole genome shotgun sequence.
ACCESSION   DATCZV010000272 DATCZV010000000
VERSION     DATCZV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286246"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1229
                     /locus_tag="VIW27_06075"
     CDS             150..1229
                     /locus_tag="VIW27_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739273.1"
                     /translation="MYFESKSGLIGCGLAALLALSPARATEILFDNGEAIDNAGLPGG
                     NGYSSNLQSNQQVFDNFAFARDSSIESVRFQMAGGTEAFRFSVYNDDAGMPGAEIFSL
                     DLEAGDYRFGPATEYSNGVNPSDIGHDFSFDLPTPLTLPPGSYWLSFLGTGNNLFRTL
                     GSGEGDGFVQRQLRSEQATGRPGNTPFRLIGQPVDDAVAVIDIKPESDDNSINPRSAG
                     VIPVAVLGSIDFDATQIRVSTVVFGPDAATPEHNGHVEDVNNDGFMDMLFHFDTRESG
                     IQCGDTEAFLRGEIFDGQSFGNLDSITTIGCDAGESPDDPIVDDDGGIATGGDAGGTA
                     LDWTMLAGLGALALFGFYRRRLTGR"
     gene            complement(1316..2200)
                     /locus_tag="VIW27_06080"
     CDS             complement(1316..2200)
                     /locus_tag="VIW27_06080"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5739274.1"
                     /translation="MTEAALEITPLHDEFGARVAGVDLSRPLDVQTFAAVDDAINRHS
                     MLLFERQSMNDAAHLDFTRRFGAPEEEHVTYYGQGKITYIGLVGNVDADGNSTTQRRV
                     KSARGNEMWHSDSSFREIPAMYSILAAYEVPDEGGETEFASARAAYRRLDPAIRVGIH
                     DKIGIHDYIYSRTSIGEDAVNDSQRTFMHPVRQRLVRQNPVTGEMNVFVGSHVKAIEG
                     MPDAEARPLIARLIDEVTRPESVYRHRWRAGDLMIWDNRCVLHRGVGYDEAKYRRRMH
                     QTRVRCPDPSLAEPLDAL"
     gene            complement(2193..3182)
                     /locus_tag="VIW27_06085"
     CDS             complement(2193..3182)
                     /locus_tag="VIW27_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5739275.1"
                     /translation="MSDILISARDLSVHFPLRGFVLGERPVVRACEGINLDIPKRSFV
                     GLVGESGSGKTTLGRAMLKAAPISHGEIVYADDEVTYDLGSLKGPQLKDYRKRAQLIF
                     QDPYAALSPRMTVRDIIAEPLEVMGLTSSREETDQRVREIAAMCRLNLEHLRRFPHAF
                     SGGQRQRISIARALVSGPKFIVADESVAALDVSIQADVLNLLNKIQAESGITFLFISH
                     DLSVIAHTCDFVAVMYLGQIVEAAPTRKLFSETAHPYTKALLSAIPSLDPDHVSKAQK
                     LEGEIPSPINPPPGCRFNTRCRYAQERCRSEVPEWREMSSGHHVACHFSEEID"
     gene            complement(3179..4180)
                     /locus_tag="VIW27_06090"
     CDS             complement(3179..4180)
                     /locus_tag="VIW27_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739276.1"
                     /translation="MSRPLISVENLTLRFRTDEGLVTAVNDVSFEIAPGEVLGLVGES
                     GSGKSVTAKALMMLNAENSVYDDDSRITLRVDDRDIDVLALKRPREQRIIRGGSISMI
                     FQEPMASFAPAITIGDQMVEQLLIHSDMTQAAAKEISIEMLARVGIPEPAQRFGQYAF
                     EFSGGMRQRAMIAMALSTKPKLLIADEPTTALDVTIQAQVIELMKDLVEEFHMSILFI
                     THDLGVIAQTADRVAVMYLGNLVETGTVRQVINSPAHPYTRGLLNSLPKLDDLDAPLT
                     PVPGDIPSPLDRPSGCVFHTRCPEYIGDVCKQQIPGNTVDSASHQVACHLATQEKSA"
     gene            complement(4177..5328)
                     /locus_tag="VIW27_06095"
     CDS             complement(4177..5328)
                     /locus_tag="VIW27_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739277.1"
                     /translation="MTDTATLEETDVKVDQSRKEAYFTAGQGQLIWSRFKKQRAAMWG
                     AGILVALILAGMFAPFLTPYDPTIKGRNPDYQNGAPNIPKFWDENGFSFRPFIYSYDR
                     TRSIETNFRWVTKVNRDKRRYVVLFPEGDEYTLLGMTFSTHLFGVDQGKIHLFGTDGT
                     GKDIFSRTFHSINTSLAVGTIGVLIAFVLALIIGGISGYYGGWIDSVLQMITDAVRTV
                     PAIPLFMAIAAFIPDTISSEGRFFIISIILGFIGWPTLARRVRTHLLTERNQEYVLAA
                     QLCGASAGHVIRRHLLPSFTSYIIVDLIISFPYMVLSETALSFIGLGLKEPVNSLGVM
                     LQNVTKADVLLNYQWNFIPVIFFITLVLAFVFVGDGLRDAADPYSDTRK"
     gene            complement(5334..6338)
                     /locus_tag="VIW27_06100"
     CDS             complement(5334..6338)
                     /locus_tag="VIW27_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008229601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739278.1"
                     /translation="MFHFVQFAATRAALAVVTLIMVSFIVFSLMELVPGDCAERYLAF
                     KNTQGNQISVADIEAERVRLGLDKPYVVRWGKWIVNAFQGEFGDSCILRLNISQLLGE
                     KFWISLAICLTSLLLAYLIAIPVGIISATSKSPIVNNSLRLVSYLGLAMPNFLLALMI
                     MLFSTVVFGDSLTGLFSKEFRDADWSYDRLIDFLSRAWLPVFILGWSATAFAIQTVRA
                     LMSDEVGKLYVLAAAARGVSGRRLLWRYPARHSLGPIINSLGFDLNRIFNELPIVALI
                     LTLTEAGSLLIEALARSNDQQMAGAIIFLLTASIVGINFITDIILALTDPRVRRSILG
                     "
     gene            complement(6459..>6663)
                     /locus_tag="VIW27_06105"
     CDS             complement(6459..>6663)
                     /locus_tag="VIW27_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739279.1"
                     /translation="VGSPESNKLGARLVENLVENLLFIGTVQVPAPIYHRNALKNFVE
                     YKTHSFEYYRSYPYRPTQWWLDE"
BASE COUNT         1309 a         2018 c         2078 g         1258 t
ORIGIN      
        1 cagccggcaa cgcctcagca gcgacggccg gctggcgtca attttccgcc gccgccaggc
       61 ttgcggccag ctatcgggca aatccgaccg gaaatcggcg gcaggctaga tttcaagcaa
      121 aatatcgagt cttgcccaat acttagggaa tgtatttcga atcgaagtca ggattgatcg
      181 gctgtggact ggcagcgctg ctggcgcttt cgccagcgcg cgcgacggag atcctgttcg
      241 acaatggcga agccatcgac aacgccggcc tccctggcgg taacggttac agcagcaacc
      301 tgcagagcaa ccagcaagtc ttcgataact tcgccttcgc ccgtgacagc tcgatcgaat
      361 cggttcgctt tcagatggcc ggtggcaccg aagcgttcag attctcggtt tataacgatg
      421 atgccggcat gcccggcgcg gaaatttttt cgctcgacct cgaggccggc gattaccgtt
      481 tcggccccgc cacggaatac tcgaacggcg tcaatccgtc cgatatcggt cacgatttca
      541 gcttcgatct gccgaccccg ctgacgctac cccccggtag ctactggctc agcttcctcg
      601 gcaccggcaa caacctgttc cgcacgctgg gcagcgggga gggagacggt ttcgtccagc
      661 ggcaattacg cagcgagcaa gcaaccggcc gcccgggcaa cacgccgttt cgactgatcg
      721 gtcaaccggt cgacgatgcg gtggcggtca tcgatatcaa gccggaaagc gacgacaact
      781 cgatcaatcc gcgcagtgcc ggcgtgattc cggtggcggt gctgggatcc atcgatttcg
      841 atgcgacgca aatcagggtg tcgaccgtgg tcttcgggcc ggacgccgcg acgcccgagc
      901 acaacggtca tgtcgaggac gtcaacaatg acggtttcat ggacatgctg ttccatttcg
      961 atacccggga aagcggtatc cagtgcggcg acaccgaagc cttcctgcgc ggcgaaatct
     1021 ttgacggcca gtcgttcggc aatctcgatt cgatcaccac catcggctgc gacgccggcg
     1081 aatcgcccga cgatcccatc gtcgacgacg acggcggtat cgctaccggg ggcgacgccg
     1141 gcggaaccgc gctggactgg acaatgctag cgggcctggg tgcgctcgcc ctgttcggtt
     1201 tctatcgccg ccgcctcacc ggccgatagt ccccggttgc ccacggatcg gcgccactcg
     1261 agcggcacgc ggggcggcca atccggggtt gccgcggcgc ggcaagagct tcctttcaca
     1321 gtgcatccag cggctcggcg agcgacgggt cgggacagcg tacccgcgtc tggtgcatgc
     1381 ggcggcggta cttcgcttcg tcgtaaccga cgccgcgatg cagtacgcag cggttgtccc
     1441 agatcatcag gtcgccggcg cgccagcggt ggcgatagac ggattcgggc cgcgtgactt
     1501 cgtcgatcag gcgtgcgatc agcggtcggg cctcggcgtc cggcatgccc tcgatcgcct
     1561 tgacatgcga gccgacgaac acattcatct cgccggtgac cgggttctgc cgcaccaggc
     1621 gctggcgcac cgggtgcatg aacgtgcgtt ggctgtcgtt gaccgcgtcc tcaccgatac
     1681 tggtgcgcga gtagatgtaa tcatggatgc cgatcttgtc gtggatcccc acccgaatgg
     1741 ccggatcgag gcgccgataa gccgcgcgcg cgctggcgaa ctcggtctcg ccgccctcgt
     1801 ccggcacttc gtaggcagcg agaatcgagt acatcgccgg gatttcgcga aacgacgagt
     1861 cggaatgcca catttcgttg ccgcgcgccg atttgacgcg ccgctgagtg gtcgagttgc
     1921 cgtcggcgtc gacattgccg accaggccga tgtaagtgat ttttccctgc ccgtagtagg
     1981 tgacgtgctc ttcctctggt gcaccgaagc ggcgggtgaa atcgaggtgc gcggcatcgt
     2041 tcatcgattg tctttcgaac agcagcatcg aatggcgatt gatcgcgtcg tcgacggcgg
     2101 cgaaggtctg gacgtcgagc ggccgcgaca ggtcgacacc agcgacgcgc gcgccgaatt
     2161 cgtcgtgcaa gggcgtgatt tcgagcgcgg cctcagtcaa tttcttcgga gaaatggcag
     2221 gcgacgtggt ggccgctgct catctcgcgc cactccggca cctcgctgcg gcagcgttcc
     2281 tgcgcgtagc ggcaacgggt attgaacctg cagcccggcg gcgggttgat cgggctggga
     2341 atttcgcctt cgagtttctg cgccttgctc acatgatcgg gatccagcga cgggatcgcc
     2401 gacagcagcg ccttggtgta gggatgcgcg gtctcggaaa acagcttgcg cgtcggcgcg
     2461 gcctcgacga tctggccgag gtacatgacc gcgacgaaat cgcaggtgtg ggcgattacc
     2521 gacaggtcgt ggctgatgaa caggaaggtg ataccgcttt ctgcctggat tttgttcagc
     2581 aggttgagca cgtcggcctg gatcgacacg tcgagcgcgg ccacgctctc gtcggcgacg
     2641 atgaacttcg gtcccgacac cagtgcgcga gcgatggaaa tgcgctgccg ctggccgccc
     2701 gagaacgcat gcgggaaacg ccgcaggtgt tcgaggttga gccggcacat ggcggcgatc
     2761 tcgcgcaccc gctggtcagt ctcctcgcgg ctggaggtca gccccatgac ttcgagcggc
     2821 tcggcgatga tatcgcgcac cgtcatgcgc ggcgacagcg ccgcgtaagg gtcctgaaag
     2881 atcagctgcg cgcgcttgcg gtaatccttg agctgcggcc ccttcagact gccgaggtcg
     2941 taggtgacct cgtcgtccgc gtatacgatt tcgccgtggc tgatcggcgc ggccttgagc
     3001 atcgcgcggc cgagcgtggt cttgccggac ccggactcgc cgaccaggcc gacgaagctg
     3061 cgcttcggga tgtcgagatt gataccctcg caggcacgca ccaccgggcg ctcgccgagg
     3121 acgaaaccgc gcaatggaaa atgcaccgac aggtcgcgtg cagaaatcag gatgtcgctc
     3181 atgccgattt ctcctgcgtg gcgagatggc aggcgacctg gtggctggcg ctatcgacgg
     3241 tatttccggg gatctgctgc ttgcagacgt cgccgatgta ctcgggacag cgggtgtgaa
     3301 acacacagcc cgacggccgg tccagcggcg acggaatatc gcccggtacc ggcgtcagcg
     3361 gggcgtcgag atcgtcgagc ttgggcagcg agttcagcag cccgcgcgtg tagggatgcg
     3421 ccggcgaatt gatcacctgg cgcacggtgc cggtctccac caggttaccg agatacatga
     3481 ccgcgacccg gtcggcggtt tgcgcgatta cgccgagatc gtgagtgatg aacaggatcg
     3541 acatgtgaaa ctcttcgacc aggtctttca tcagctcgat gacctgtgcc tggatggtca
     3601 cgtccagtgc cgtggtcggc tcgtcggcga tcagcagttt cggcttggtc gacagcgcca
     3661 tcgcgatcat cgcgcgctgg cgcataccgc ccgaaaactc gaaagcgtac tggccgaagc
     3721 gctgcgccgg ttcgggtata ccgacgcgcg ccagcatttc gatcgagatc tccttcgccg
     3781 cggcctgcgt catgtccgag tgaatcagca gttgctcgac catctggtcg ccgatcgtaa
     3841 tcgccggcgc gaaactcgcc atcggttcct ggaagatcat cgaaatcgag ccgccgcgaa
     3901 tgatgcgctg ctcgcgcggc cgcttcagcg ccagtacgtc gatatcgcga tcatcgacgc
     3961 gcagcgtgat gcggctgtcg tcgtcgtaga ccgagttttc ggcgttcagc atcatcagcg
     4021 ccttggcggt caccgacttg ccggaaccgc tctcgccgac cagccccagc acttcgcccg
     4081 gcgcgatttc gaagctcaca tcattgacgg ccgtcaccag gccttcatcg gtcctgaagc
     4141 gcagcgtcag gttctctacc gaaatcaggg gccggctcat ttgcgggtat ccgaataggg
     4201 atccgcggca tcgcgcaacc cgtcgccgac gaacacgaag gcgagcacca gggtgatgaa
     4261 aaagatgacc ggaataaaat tccactgata attgagcagc acgtcggcct tggtcacgtt
     4321 ttgcaacatg acgccgagcg agttgaccgg ttccttcaat cccagaccga taaagctgag
     4381 cgcggtctcc gacaaaacca tgtacggaaa cgagatgatc aggtcgacga tgatgtaact
     4441 ggtgaagctc ggcagcaggt gacggcgtat gacatgcccc gccgatgctc cgcaaagctg
     4501 cgccgcgagc acgtattcct ggttacgttc ggtcagtaaa tgggttcgaa cacgacgcgc
     4561 cagcgtcggc cagccgatga aaccgaggat gatcgaaatg atgaagaacc gtccctcgga
     4621 actgatcgta tccgggatga aggccgcgat cgccatgaac agcgggatcg ccggcacggt
     4681 tcgcaccgcg tcggtgatca tttgcagcac ggaatcgatc cagccgccgt aatatccgga
     4741 tatgccgccg atgatcaatg ccagcacgaa ggcgatgagc acgccgatgg tgccgaccgc
     4801 cagcgaagtg ttgatcgagt gaaaggtccg cgaaaagata tccttgccgg taccgtcggt
     4861 gccgaacagg tgaatcttgc cctggtcgac gccaaacagg tgcgtgctga aggtcatgcc
     4921 gagcagtgtg tactcgtcgc cctccggaaa cagcaccacg taacgccgct tgtcgcggtt
     4981 gaccttggtc acccagcgga aattggtttc gatcgagcgg gtgcggtcgt agctgtaaat
     5041 aaacggtcga aacgagaagc cattttcatc ccagaacttc ggaatattcg gcgcgccgtt
     5101 ctggtaatca gggttacgcc ccttgatggt gggatcgtag ggcgtcagga aaggtgcgaa
     5161 catgcccgcc agaatcaatg cgaccagaat gccggcaccc cacatggccg cgcgctgctt
     5221 cttgaaccgg gaccagatca actgcccctg gccggcggta aaataggctt ccttgcggct
     5281 ctggtcgact ttgacgtcgg tttcttcgag ggttgcggta tcggtcatcg tgcctatccc
     5341 aggatactgc ggcgaacccg cggatcggtc agcgccagga tgatatcggt aatgaaattg
     5401 ataccgacga tgctggcggt gagcaggaaa ataattgcgc ccgccatctg ctggtcgttc
     5461 gagcgcgcca gtgcctcgat caacagggac ccggcctcgg taagcgtcaa gatcagcgcg
     5521 acgatcggca actcgttgaa gatgcgattc agatcgaatc cgagcgagtt gatgatcggc
     5581 ccgagcgagt gcctggccgg gtagcgccac aacagtcggc gcccgctgac gccgcgcgcc
     5641 gccgcggcca gtacatagag ttttccgact tcgtcggaca tcagcgcgcg cacggtctga
     5701 atcgcgaacg cggtcgccga ccagccgagg ataaagaccg gtaaccaggc gcgcgacaga
     5761 aaatcgatta gcctgtcgta agaccaatcg gcatcgcgga attccttgga aaacaacccg
     5821 gtcaggctgt cgccgaaaac cacggtcgaa aacagcatga tcattagcgc gagcaggaaa
     5881 ttcggcatcg ccaggccgag gtaactgacg agccgcaggc tgttgttgac gatcggactt
     5941 ttcgaggtcg cggagatgat acccacgggt atcgcaatca ggtaggcaag caacagcgac
     6001 gtcaggcaga tagccagact gatccagaac ttctctccca gcaattgaga gatattgagg
     6061 cgcaggatgc agctgtcgcc gaattccccc tggaaagcgt tgacgatcca cttgccccag
     6121 cgcaccacgt aaggtttatc cagtccgagc cggacgcgtt ccgcttcgat atcggcgacc
     6181 gatatctggt tgccctgggt gtttttaaac gcgaggtaac gctcggcgca gtcgcccggc
     6241 accagctcca tcaggctgaa aacgatgaat gacaccatga tcagcgtgac aaccgccagc
     6301 gcggcacgcg tagcagcgaa ctgaacgaag tggaacatta ctgtcttcgt gccccccgat
     6361 ttactactcg gcgtgtcgcg tcaaacccgc cggactgctt tattatctgg ctgcgggcaa
     6421 atcctgcccg cagccatttt tatatcaacc tcgcgcgatt actcgtccag ccaccattgg
     6481 gtcggccggt acgggtagga gcggtaatac tcgaacgagt gcgtcttgta ctcgacgaag
     6541 tttttcagcg cgttgcgatg gtagatcggc gccggtacct gcacggtacc gatgaacagc
     6601 aggttttcga ccaggttctc gaccagccgc gcaccgagtt tgttcgattc cggcgagccc
     6661 acc
//
LOCUS       DATCZV010000273         4672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_286406, whole genome shotgun sequence.
ACCESSION   DATCZV010000273 DATCZV010000000
VERSION     DATCZV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286406"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VIW27_06110"
     CDS             complement(<1..306)
                     /locus_tag="VIW27_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739280.1"
                     /translation="MSERLAVFYDERVLEHDTGSGFFEAAASPYLVHREPHPENRLRL
                     ENMVAVLREGPIAAALDWRRAPAATEQDLERFHDPAYIDELASIPRDEPRVMSSTTVF
                     "
     gene            410..1648
                     /locus_tag="VIW27_06115"
     CDS             410..1648
                     /locus_tag="VIW27_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5739281.1"
                     /translation="MGDNRDRVLQQCRPAMELGFSLAEYDQRLAAIRARMAADNIDLL
                     WLMAPESLYYVSGYACEWYQAQSPKQWPATSGIAVHVDHDHFILFDTPSEQVMSRFVT
                     CSRDTRIFPIDNRRDGIGFIIDELQAAGWLDGTVAMELHSYRPNPVVSARFRAAFEAG
                     GAAVVDGSDILRELRWIKSPQEIAYIEQAARIADIGIAAARDTIRAGVTELEVYGEMI
                     RAMAKAGGENPGITLPVLSGAKANTGHSLASRKVIMAGEQVNIDVCGVYWRYHANAAR
                     SFYVGEPPRDVRDYHARSAGVFDVIAGILEPGLTVGELVRTVRAYYDEAGIWSDAGWV
                     GGYELGIGFPPDWVGNFVYEMSHTDSECVFEPRTCVNFESQFFSPRLSGITYYICTLL
                     FKDDRAELPVQAPRELLVID"
     gene            1964..4528
                     /locus_tag="VIW27_06120"
     CDS             1964..4528
                     /locus_tag="VIW27_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5739282.1"
                     /translation="MAQFPDKANVVIVGLGGIVGASVMHHLIERGWEDIVGIDMTSIP
                     TDCGSTSHASDFCYATDHSKFTCYTTHYSIEFFDKIGHYERCGGLEVARVGDDDRMME
                     LKRKVASGKAFGTRVKMISAAEARQKFPLLEEDMIQGAMWDPDAGLVKPRSQTVVGIL
                     VDEAVKTGKAQSWANTRATGLDIKDGRIVGVDTSRGYIAADTVVVCTGLWGRSIAEMA
                     GEDLPVMPVDHPLSFFGPYDEFAGTGKDIGYPLLRDQGNSAYMRDTGDPTTPEGGQIE
                     WGYYEEKEPRLVHPRDLPEKGETHWSPSQRDLELEQIMEPLERAMELTPILGELGFKD
                     DHSFNGLLQVTSDGGPSIGESSQTRGLWYAVAVWVKDAPGIAKVLVDWMTDGVAEMDF
                     FGIDTARHYPIQKTQSYIHDRCYESAFKIYNPAVHNREPYTKGRNLRRSPFWAREQEL
                     GGYFMELAGWERAHGYASNEHLLEKYAERVPVREHEWDARHFWRVSNAEQLAMSENVG
                     MINLSHFAVFDVIGPDAEALMEYACVARVGGDTPIGKGVYTHFLDKRGGVRADLTVIR
                     HAEDRFRVIDGADAGHRDYIWLKRMAEDNGWQVYIDDRSDHVACLGVWGPNARQTLQA
                     IADDPAAWDDENFPFAATRRLTLKGIPVEAFRISYVGEQGWELHVDFSYGLSLWDLVY
                     AQGATPVGIETYANSRRMEKSLRLQNADLLTEYNLYEAGLARPVVKQADFHGKAPYLE
                     QRELDKQAAYLCTLVMDDNTDSNGIKRYPVGQLPLIDPASGEVPIDTKGRRSYVSSIA
                     YGPSLGKNIGLGYLPHEYCEEGRELLTEYLGEQFTMRVAAVGYQPLYDPENKLPRS"
BASE COUNT          897 a         1535 c         1478 g          762 t
ORIGIN      
        1 gaacaccgtg gtgctcgaca tgacgcgggg ctcgtcgcgc gggatcgagg ccagctcgtc
       61 gatataggcg ggatcatgga aacgttccag gtcttgctcg gtggccgccg gcgcgcgccg
      121 ccagtcgagc gccgcggcga tcgggccctc gcgcagcacg gcgaccatgt tctcgaggcg
      181 caaccggttc tcgggatgag gctcgcgatg caccaggtag ggcgacgcgg cagcctcgaa
      241 aaacccgctg ccggtgtcgt gctcgagcac gcgttcatcg tagaaaaccg ccagccgctc
      301 gctcattcgt caccctccgc tgcttttcgt ctgacaggaa tatgccggcc tgatattctt
      361 gaccgtccgg cgcgggtcat cgggccgcgc agctgccacg gagaatatca tgggagacaa
      421 ccgcgatcgc gttctgcagc aatgccggcc ggcgatggag ctcggttttt cgctggccga
      481 gtacgatcag cgcctggccg cgattcgcgc gcgcatggcc gcggacaaca tcgacctgct
      541 gtggctgatg gccccggaga gtctttacta tgtcagcggc tacgcctgcg aatggtacca
      601 ggcacaaagc ccgaaacagt ggcctgccac cagcggcatc gcggtgcacg tcgaccacga
      661 tcatttcatc ctcttcgata cgccgagcga gcaggtcatg agccgcttcg tgacctgctc
      721 gcgggacacg cgcatcttcc cgatcgacaa ccgccgcgac ggtatcggtt tcatcatcga
      781 cgaactgcaa gccgctggct ggctcgacgg caccgtggct atggagttgc acagctaccg
      841 gccgaacccg gtggtcagcg cgcgctttcg cgccgctttc gaggctggcg gcgcggcggt
      901 cgtcgatggc agcgacatcc tgcgcgagct tcgctggatc aaatcgccgc aggagatcgc
      961 ctatatcgag caggccgcgc gcatcgccga tatcggcatc gcagcggcgc gcgatacgat
     1021 tcgcgccggt gtcaccgaac tcgaggtcta cggcgagatg attcgagcga tggcaaaagc
     1081 cggtggcgaa aatccgggga tcacgctgcc ggtgctgtcc ggcgccaagg ccaacaccgg
     1141 ccattcactc gccagccgca aggtgatcat ggcgggcgaa caggtcaaca tcgatgtctg
     1201 cggcgtctac tggcgctacc atgcgaatgc cgcacgctcg ttctacgtcg gtgaaccgcc
     1261 ccgcgacgtg cgcgactacc atgccaggtc agcgggggta ttcgacgtca tcgccggcat
     1321 cctcgagccg gggctcacgg tcggcgagct ggtgcgcacg gtgcgcgcct actacgacga
     1381 ggccggcatc tggtcggacg ccggctgggt cggaggctac gaactcggca tcggatttcc
     1441 gcccgactgg gtcggcaatt tcgtctacga aatgtcgcac accgattcgg agtgcgtatt
     1501 cgagccgcgc acctgcgtca acttcgagag ccagtttttc agcccacggc tgtccggtat
     1561 cacctactac atctgcacgc tgctgttcaa ggacgaccgt gccgagctgc cggtgcaggc
     1621 gccgcgtgaa ctgctggtca tcgattaagc cggcgaaaac cgtgtcgaag tcgctaatca
     1681 tgaagaggat gacgtagcga tataaaagac tgcaggcgac aactcttatg cttgcgagta
     1741 acgcgctcgg cgaccgaaat cggtgtttgc ggccgttaca gggcagccct ggccggcagc
     1801 tgaaatgggt ggagatgcga aaaccgcgtg tttatactgg ccgttccgct gccgtaagta
     1861 tacgcatcgc cgggcccgag attcgaacgc gcgtaaacgt cgagtaacag atctagatga
     1921 aaatcggcgc cggtgacggc cggcagccac tgggagaaaa gccatggcac aatttccgga
     1981 caaagcgaac gtcgtcatcg tcggcctcgg cggcatcgtc ggcgcgtcgg tgatgcacca
     2041 cctgatcgag cgcggctggg aggatatcgt cggcatcgac atgacctcga tcccgaccga
     2101 ctgcggctcg acctcgcacg cctccgattt ctgctacgcc accgaccaca gcaagttcac
     2161 ctgctacacc acccactaca gcatcgagtt cttcgacaag atcggccact acgagcgctg
     2221 cggcggcctc gaggtcgcgc gcgtcggcga cgacgaccga atgatggagc tgaagcgcaa
     2281 ggtcgcctcc ggcaaggcct tcggcacccg cgtgaagatg atcagcgccg ccgaggccag
     2341 gcaaaaattc ccgctgctcg aagaagacat gatccagggg gcgatgtggg acccggacgc
     2401 cggcctggtc aagccgcgct cgcaaaccgt ggtcggcatt ctcgtcgacg aagcggtgaa
     2461 gaccggcaag gcacaatcct gggccaatac ccgggcgacc ggcctcgaca tcaaggacgg
     2521 ccgcatcgtc ggcgtcgaca ccagccgcgg ctacatcgcc gccgataccg tcgtcgtctg
     2581 caccgggctg tggggccgca gcatcgccga gatggccggc gaggacctgc cggtgatgcc
     2641 ggtcgatcac ccgctgtcgt tcttcggtcc ctacgacgaa ttcgccggca ccggcaagga
     2701 tatcggctac ccgctgctgc gcgaccaggg caactcggcc tacatgcgcg acaccggcga
     2761 cccgaccacg cccgagggcg gccagatcga gtggggctac tacgaagaga aggaaccgcg
     2821 cctggtgcat ccgcgcgacc tgccggaaaa aggcgagacc cactggtcgc cgtcgcagcg
     2881 cgacctcgaa ctcgaacaga tcatggagcc gctggagcgg gcgatggaat tgacaccgat
     2941 tcttggcgag ctcggcttca aggacgatca ctcgttcaac ggcctgctgc aggtgacttc
     3001 cgacggcggt ccgtcgatcg gcgaatcctc gcagacccgc ggcctgtggt acgcagtcgc
     3061 ggtgtgggtc aaggacgccc ccggcatcgc caaggtgctg gtcgactgga tgaccgacgg
     3121 cgtcgccgag atggatttct tcggtatcga caccgcgcgc cactacccga tccagaagac
     3181 gcagtcttat atccacgacc gctgctacga atccgcgttc aagatctaca acccggcggt
     3241 gcacaaccgc gaaccctata ccaagggccg caacctgcgc cgcagcccgt tctgggcgcg
     3301 cgagcaggag ctcggcggct acttcatgga gctcgccggc tgggaacgcg cgcacggcta
     3361 cgcgtccaac gagcacctgc tggagaaata cgccgagcgc gtaccggtgc gcgagcacga
     3421 gtgggacgcg cgccacttct ggcgcgtctc caacgccgag caactggcga tgtcggaaaa
     3481 cgtcggcatg atcaacctgt cgcacttcgc ggtgttcgac gtcatcggcc ccgatgccga
     3541 ggcgctgatg gaatacgcct gcgtcgccag ggtcggcggc gatacgccga tcggcaaggg
     3601 cgtctacacc cacttcctcg acaagcgcgg tggcgtgcgc gccgacctga ccgtgatccg
     3661 ccacgccgag gaccgctttc gcgtcatcga cggcgccgac gccgggcacc gcgactatat
     3721 ctggctgaag cgcatggccg aggacaacgg ctggcaggtc tacatcgacg accgcagcga
     3781 ccatgtcgcc tgcctcggcg tctggggacc gaacgcgcgc cagaccctcc aggcgatcgc
     3841 cgacgacccg gccgcctggg acgatgaaaa cttcccgttc gcggcgactc gccggctgac
     3901 gctgaaaggc attccggtcg aagccttccg catttcctac gtcggtgagc agggttggga
     3961 actgcacgtc gatttcagct acggcctgtc gctgtgggac ctggtctatg cgcagggtgc
     4021 gacgccggtc ggtatcgaga cctacgccaa cagccggcgc atggaaaaaa gcctgcgact
     4081 gcagaatgcc gacctgctga ccgagtacaa cctctacgag gctggtctcg cgcggccggt
     4141 ggtcaagcag gccgacttcc atggcaaagc gccctacctc gagcagcgtg aactcgacaa
     4201 gcaggcggcc tacctgtgca cgctggtgat ggacgacaac accgactcga acggtatcaa
     4261 gcgctacccg gtgggccagc tgccgctgat cgacccggcg agcggcgagg tgccgatcga
     4321 cacgaagggg cgccgctctt acgtcagcag catcgcctac ggcccgagcc tcggcaagaa
     4381 catcggtctc ggttacctgc cgcacgaata ctgcgaggaa ggtcgcgaac tgctcaccga
     4441 gtacctcggc gaacagttca cgatgcgcgt cgccgcggtc ggctaccagc cgctctacga
     4501 cccggaaaac aagctgccgc gcagctgatc aaagaggggg cggttctagt ccgcccccgg
     4561 catcgacgtt catttcatcg ccagcaatac aacggcgaat atcacgaata cgcaggccac
     4621 ggccacgctc tccagcccga tcgatgccgg cccgatcttt tcgcgcctga gc
//
LOCUS       DATCZV010000274         6674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_127729, whole genome shotgun sequence.
ACCESSION   DATCZV010000274 DATCZV010000000
VERSION     DATCZV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127729"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VIW27_06125"
     CDS             <1..531
                     /locus_tag="VIW27_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5739283.1"
                     /translation="MANSVVFLSQAKNEAYDGHCDADDVLVVQLAGRKTWQLFKPQQR
                     RYAGISDQSEEQLGPVQHELVMRPGDALYVRAGVPHRCITSGAFSMHMAFDLVDQTPP
                     IASITAQAANLYNHACELPHSPPSSVIDRFVSILQSEDFRQALAEATEIKRADIAGFR
                     EMLGRSSAVRSLVKLK"
     gene            complement(536..1777)
                     /locus_tag="VIW27_06130"
     CDS             complement(536..1777)
                     /locus_tag="VIW27_06130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5739284.1"
                     /translation="MKSSIHVLGAGIVGISCALELQRRGYRVTLIDRRGPGKEASSGN
                     AGLLSYSNITPLASPALLPRLPKLMTNLDNDFRLHYPHLLQLMPWMLRFMWRCRRDVY
                     LRDGAAMNDLTQASVALHRQWIDEFGAGSLAAHGGGLKLYRERRSFRRDALERELLQR
                     CGVRYRLLDRRQIEELEPDLQRVFEQGVLIEDTISIRDPEALCRSYAQAFVAAGGRLE
                     LDEARDLQPSGGGWELVTGRGREQVEKLVICLGAATPDLLRPLGYPNPLAIERGYHMI
                     LEPAAGKHLGRPIFDVDASYVMSPMAMGYRVSSGSNLVHAETRPDPRQLLRLLPRARE
                     AFPLGRELLSEPWMGRRPTLPDTLPIIGPAPRHSNLWLAFGHSHMGFTLGPISGLLIA
                     NFVDGSEHPFAVAPCDPRRYL"
     gene            complement(1788..2684)
                     /locus_tag="VIW27_06135"
     CDS             complement(1788..2684)
                     /locus_tag="VIW27_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY5739285.1"
                     /translation="MVDSLRSANRLARASLYEWHYFTADGRPVSASCGISVPARPLAE
                     ASAIDTLIIVAPNTAQEFDDAEVLRQLKALDRRQVNLGSASSGSFILARAGLLDGTRC
                     TIHWENIPVFRELYPQLEVAFTLYEIDRRRFTCSGGTAALDMMLKLIDARHGRELAQQ
                     ISQQFHHDRIRSQIDSQQMADRMDLAMHAPKLIDVIQLMENHIENPLPLPAIAARCKL
                     SLRQIERLFHKYRDVTPSQYYLALRLKHAKQLLLNTNRSVIDISIATGFETQSYFTAC
                     YRKHFGSSPRTHRSQVATDSKR"
     gene            2959..4524
                     /locus_tag="VIW27_06140"
     CDS             2959..4524
                     /locus_tag="VIW27_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5739286.1"
                     /translation="MSEANRPARGRRGRGGREERRSAASAAPVSAPYITRKIPCFEVV
                     DEEGLEIIERNADTILEEIGIEFRDMPEALEILRQGGAEIDGVTARFPRGLCRSIIQG
                     SAPAEYTQHARNPERNVRIGGKHTVFVPAYGSPFVFDIDKGRRYATIEDFQNFVKLAY
                     ASPSLHHSGGTICEPVDIPVNKRHLDMVYSHIKYSDKPFMGSVTAPERALDTVEMIKI
                     IAGENFLDPETGRPRTYATSLINANSPMTFDDTMLGAAKVYAENNQAVIITPFILAGA
                     MSPVTVAGTAAQSLAEALAGMTFVQLVNPGAPMIFGSFASSMSMQSGAPTFGTPEPAL
                     VLYIMAALARRLGVPFRSGGGLCASKLPDAQAASEAANTLLPAVLAGVNFVLHTAGWM
                     EGGLAMGYEKFIMDADQAAMMAIQLGGVDLSENGQAMDAIREVGPGVHFLGCNHTQRN
                     FKTAFYRSPITDNNSFEQWEAEGGLDHAQRANARYKQLLAEYQPPALDPAIDEALIEF
                     VERRKQSMPDSMV"
     gene            4739..5461
                     /locus_tag="VIW27_06145"
     CDS             4739..5461
                     /locus_tag="VIW27_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY5739287.1"
                     /translation="MSRWILLLLVFCPAILHAAEHDAGGPAGTWKGQGELGFSSTSGN
                     SDSENLNASLRLSRETDAWKHSAALDSLQAKTDGETSADRFEFRQRSEYGLGEVSYLY
                     GQFRYEDDEFSGYQYQASATVGAGSRLLTTDRHRVDVSAGLGMRRIESSADGTTDSEG
                     IFGSDLAYEYLISEFAVLKARLLLESGDDNTYSESETALRTRIDGALSAKIGYLVRHN
                     SKVPSGIENTDRLATVSLVYDF"
     gene            5960..>6674
                     /locus_tag="VIW27_06150"
     CDS             5960..>6674
                     /locus_tag="VIW27_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020288111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY5739288.1"
                     /translation="MNDATSPSRPEHFGFLQVPGYSMIAFASAIEPLRMANRHSGRDL
                     YRWSVFTIDGLPEKASNGLEITPHDSIDKAGDISILFVCGGVDIADAWSRQLQFALRR
                     IAKRNGVRLGALCTGSYLLARAGLLDGYKCTIHFENIASLREEFPEVLVSDDLYLFDR
                     DRITCAGGQASMDMMLKLIEKSHGNALVTPISEQFMCERIRGSNDRQRVPLHLSLGSN
                     QPKLTEAVTLMEANIEEPIS"
BASE COUNT         1298 a         2170 c         2058 g         1148 t
ORIGIN      
        1 atggccaaca gcgtggtgtt cctgtcgcag gcgaagaacg aggcctacga tggccactgc
       61 gacgccgacg acgtgctggt ggtgcagctc gccggcagga aaacctggca attgttcaag
      121 ccccagcagc gtcgctacgc gggcatcagc gatcaaagcg aggagcagct cgggccggtc
      181 cagcacgagc tggtgatgcg tcccggcgac gcgctctacg tgcgtgccgg ggtaccgcac
      241 cgctgtatta cctcgggcgc gttcagcatg cacatggctt tcgacctggt cgaccagaca
      301 ccgcccatcg ccagtatcac ggcacaggcg gccaacctct acaaccatgc ctgcgaatta
      361 ccccactcac cgccatcgag cgtgatcgat cgcttcgtat cgatcctgca gtcggaggat
      421 ttccggcagg cgctggccga ggcgaccgag atcaagcgcg ccgacatcgc cggctttcgt
      481 gaaatgctcg gccgctcgtc ggcggtcagg agcctggtca agctcaagta gcagcttaca
      541 ggtagcggcg cgggtcgcag ggggcgaccg caaacgggtg ctcgctgccg tcgacgaagt
      601 tggcgatcag caggccgctg atcggaccga gcgtgaagcc catgtgcgaa tgcccgaagg
      661 cgagccacag gttcgagtgg cgtggcgccg ggccgatgat cggcagcgta tccggcaagg
      721 tcggccgtcg gcccatccac ggctcgctga gcagctcgcg gccgagcgga aaggcttcgc
      781 gggcgcgcgg cagcaaacgc aacagctgcc gcgggtcggg ccgggtctcg gcatgcacca
      841 ggttgctgcc gctgctgacc cggtagccca tcgccatcgg cgacatcacg tagctggcgt
      901 cgacatcgaa gatcggccgg cccaggtgct tgccggcggc cggctccaga atcatgtggt
      961 aaccacgctc gatggcgagc ggattcgggt atcccagcgg acgcagcaag tcgggcgtcg
     1021 ctgccccgag acagatcacc agcttctcga cctgctcgcg tccccgcccg gtgaccagtt
     1081 cccagccgcc gccggagggc tgcaaatccc gtgcctcgtc aagctcgagg cggccgccgg
     1141 cagccacgaa cgcctgcgca taactgcgac acagcgcttc cgggtcgcga atcgaaatcg
     1201 tatcctcgat cagcacgcct tgctcgaata cgcgctgcag atcgggttcg agttcctcga
     1261 tctggcggcg atcgagcagt cgatagcgca cgccgcagcg ctgcagcaat tcgcgctcca
     1321 gcgcatcgcg gcgaaagctt cgccgctccc ggtaaagctt gagaccgccg ccgtgagcgg
     1381 ccagcgaacc ggcaccgaac tcgtctatcc attgccggtg cagcgccacc gaagcctgcg
     1441 tcaggtcatt catcgcggcg ccgtcgcgca ggtagacatc gcgccggcag cgccacatga
     1501 aacgcagcat ccacggcatc agctgcagca ggtgcgggta atgcaggcga aaatcgttat
     1561 ccaggttggt catcagcttc ggcagtcgcg gcagcagcgc cgggctcgcc aacggcgtga
     1621 tgttgctgta actcagcagg ccggcgttgc ccgacgacgc ctccttgccg ggcccgcggc
     1681 gatcgatcag ggtgacacga taaccgcgcc gctgcagttc cagcgcgcaa ctgatgccga
     1741 ctatgccggc acccagcacg tgtatcgagg atttcatcgc ttatcgtcta ccgcttgctg
     1801 tcggttgcca cctgcgagcg gtgggtgcgt ggtgaactgc cgaaatgctt acgatagcag
     1861 gcggtgaaat acgactgggt ctcgaagccg gtggcgatgg aaatatcgat gacgctgcga
     1921 ttggtattga gcagcagctg cttggcatgc ttcagccgca gcgccaggta gtactggctc
     1981 ggcgtgacat cgcgatactt gtgaaacagg cgctcgatct ggcgcagcga cagcttgcag
     2041 cgggcggcga tcgccggcag cggcagcgga ttttcgatat ggttctccat cagctggatc
     2101 acgtcgatca gcttcggcgc gtgcatcgcc aggtccatgc gatcggccat ctgctgggaa
     2161 tcgatctggc tgcgaattcg atcgtggtgg aattgctgcg aaatctgctg cgcgagttcg
     2221 cggccgtgac gggcatcgat cagcttcagc atcatgtcga gcgcggcggt gcccccggag
     2281 caggtgaacc ggcgccgatc gatttcatag agcgtaaagg ccacttcgag ttgcgggtag
     2341 agttcgcgga acaccggtat gttctcccag tgaatggtgc agcgagtgcc gtcgagcaat
     2401 cccgcgcgcg ccaggataaa actgcccgaa ctcgccgagc cgagattcac ctgccggcga
     2461 tcgagagcct tgagctgccg cagcacctcg gcatcgtcaa actcctgggc ggtattcggc
     2521 gcgacgatga tcagcgtatc gatagcgctt gcctcggcaa gcgggcgagc cggcacgctg
     2581 atgccgcagg aagccgatac cggccggccg tcggcagtga agtagtgcca ttcatacagc
     2641 gatgcgcgcg ccaggcggtt ggccgagcgc agtgaatcga ccacgcaact gaacgggatt
     2701 atcgggaatc cgtcgattag aagtatgccg aattgttcgc tgtcgtcgct gatagccagc
     2761 ctcgtatgcc gcgccttgcg gaccgttgcc gcattctagc gaaaatgcgt cgctcgacaa
     2821 attcggataa tattccggcg actcgagatg gcgcgggcaa atatttccgg gtcgctcgca
     2881 gatcagccgg gaaaatcgcc aaaaacgata ctggtcgatt gacgcccgca cagaggctcg
     2941 aaaactgagg tagcgacaat gagtgaagca aaccgacctg ccaggggacg ccgcggccgc
     3001 ggcgggcgcg aagaacgacg cagcgccgct tccgccgctc cggtcagcgc gccttacatc
     3061 acgcgcaaga ttccctgttt cgaggtcgtc gacgaggagg ggctggagat catcgagcgc
     3121 aatgccgata ccatcctcga ggaaatcggc atcgagttcc gcgacatgcc cgaggcgctc
     3181 gaaattctca ggcagggcgg cgccgagatc gacggcgtta ctgcccgctt tccgcgcggc
     3241 ctgtgccgct ccatcattca aggctcggcg ccagccgaat acacccagca cgcgcgcaac
     3301 cccgagcgca acgtgcgcat cggcggcaag cacacggtgt tcgtgccggc ttacggcagc
     3361 ccgttcgtgt tcgatatcga caagggccgc cgctacgcga cgatcgagga tttccagaat
     3421 ttcgtcaagc tggcctacgc ctcgccctcg ctgcaccatt ccggcggcac gatctgcgaa
     3481 ccggtggata ttccggtcaa caagcgtcat ctcgacatgg tctactcgca catcaagtat
     3541 tccgacaagc cgttcatggg ctcggtaacc gccccggagc gcgccctgga cacggtggag
     3601 atgatcaaga tcatcgccgg cgaaaatttc ctcgatcccg agaccggccg gccgcgcacc
     3661 tacgccacca gcctgatcaa cgccaactcg ccgatgacct tcgacgacac catgctcggc
     3721 gcggccaagg tctacgcgga gaacaaccag gccgttatca tcaccccgtt catcctcgcc
     3781 ggcgcgatgt cgccggtcac cgtggccggc accgccgcgc aatcgctggc cgaggcgctg
     3841 gccggcatga ccttcgtgca gctggtcaac ccgggtgcgc cgatgatctt cggcagcttc
     3901 gccagctcga tgtcgatgca gtccggcgcg ccgaccttcg gtaccccgga gcccgcactg
     3961 gtgctctaca tcatggccgc gctggcgcgc cgcctcggcg tacccttccg ctccggtggc
     4021 ggactctgcg cctccaagct gcccgacgct caggccgcct cggaggccgc caacacgctg
     4081 ctgccggcgg tgctcgccgg cgtcaacttc gtgctgcaca ccgccggctg gatggagggc
     4141 gggctcgcca tgggttacga aaaattcatc atggatgccg accaggcggc gatgatggcg
     4201 atccagctcg gcggcgtcga cctgtcggaa aacggccagg cgatggacgc gattcgcgag
     4261 gtcggccccg gcgtgcactt cctcggctgc aaccatacac agcgcaattt caagaccgcg
     4321 ttctaccgct cgccgattac cgacaacaac agcttcgagc aatgggaagc ggagggcggc
     4381 ctcgatcacg cacagcgcgc caacgcacgc tacaagcaac tgctcgccga gtatcagccg
     4441 ccggcactcg acccggccat cgacgaggca ctgatcgagt ttgtcgagcg gcgcaagcaa
     4501 tcgatgcccg actcgatggt ttagcaaaca tggacaccgt gctagcggtg tcctcatcat
     4561 aaaagtcatc gctcaccaaa gcgggaactt tgcaatggtg gcgcactgca tgattcgcac
     4621 cgatagcaag agatcgcgca cggaattcgt gcggattgaa cgaaaggccg ccgatttttt
     4681 atcgccagtg agcatctgtc acccttcgtc atcatcgatt tttcgatcag gagttagcat
     4741 gtcgcgctgg atccttttgc tgctggtatt ctgcccggcg attctgcacg ctgccgaaca
     4801 cgacgcaggc gggccggcag gtacctggaa aggccagggc gagctcggct tcagctcgac
     4861 atccggtaat tcggattccg agaacctcaa cgccagcctc aggctgtcgc gcgagacgga
     4921 cgcctggaag cactcggctg cgctggatag cctgcaggcg aagaccgatg gcgaaaccag
     4981 cgccgaccgt ttcgagttcc ggcaaagatc cgaatacggg ctcggcgaag tctcctatct
     5041 ctacggccaa tttcgctacg aagacgacga gttcagcggt taccagtacc aggcctcggc
     5101 gacagtcggc gcgggatccc gcctgctcac aaccgacagg caccgcgtcg acgtctcggc
     5161 cggcctcggt atgcgccgca tcgagagcag tgccgacggc accaccgaca gcgagggaat
     5221 cttcggctcc gaccttgcct acgaatacct gatcagcgaa tttgccgtcc tgaaggcgcg
     5281 attactgctc gaaagcggcg acgacaatac ctactcagag tcggaaaccg cgctacgaac
     5341 ccgtatcgac ggcgcgctgt cggcaaagat aggctacctg gtaaggcaca attcgaaggt
     5401 cccgtccggc atcgaaaaca ccgaccgcct ggcgacggtt tcgctggtct acgacttcta
     5461 actcgactcg acccgccgcg gccggcgagc acggcggctt cgcgaagcga cgcacggccc
     5521 ggcgatcatc ggtcgcgcac cagccacacg ctgcccacca ccctcttgcc ggccgctcac
     5581 cgccgtcgaa gcccgcgcca aacgttgcgg cggaaggttg tattcgcggg ttatgacggg
     5641 caggttcagt aaccgaatgg gccgcaggcg ttgcagcttc gcgttaccga tccccagcgg
     5701 ttgcgccgca tcaccgagca cctggtcgct gttccacagg cgcaagaccg gttgccaggc
     5761 gctacttgtg gcagcgcgaa aagtaagcgg gcacccccgg gagcggcacc gcgcgcaccg
     5821 tcgacggcgt ccggaggccg ttcgcaggca tcgtcgacag accaaaaatc acgtgcattg
     5881 acccggtata gcgtctagta ttttggcgga tcagctaacc tacagtcatt tttgagccga
     5941 atatcatcca atcgattcca tgaatgacgc aacttcccca tcccggcccg agcatttcgg
     6001 cttcctgcag gtacccggct actcgatgat cgccttcgcc tcggccatcg agccgctgcg
     6061 catggccaac cggcacagcg gccgcgacct gtatcgctgg agcgttttta ccatcgacgg
     6121 tttgcccgaa aaggccagca acgggctcga aatcacgccc cacgacagca tcgacaaggc
     6181 cggcgatata tccatcctgt tcgtctgcgg cggcgtcgat atcgccgatg cctggagccg
     6241 gcagctgcag ttcgcgctgc gccgcatcgc caagcgcaat ggcgtcaggc tcggcgcgct
     6301 atgtaccggc agctacctgc tggcgcgggc cgggctgctg gacggttaca agtgcacgat
     6361 ccacttcgag aacatcgcca gcctgcgcga agaatttccc gaggtgctgg tatccgacga
     6421 tctctacctg ttcgaccgtg atcgcatcac ctgcgccggc ggtcaggctt cgatggacat
     6481 gatgttgaag ctgatcgaga aaagccatgg taacgcgctg gtcacgccga tctccgagca
     6541 gttcatgtgc gagcgcattc gcggctcgaa cgaccgccag cgcgtaccgc tgcacctgtc
     6601 gctgggatcg aaccagccga aactgaccga ggccgtgacg ctgatggaag ccaatatcga
     6661 ggaaccgatc agcc
//
LOCUS       DATCZV010000275         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_74710, whole genome shotgun sequence.
ACCESSION   DATCZV010000275 DATCZV010000000
VERSION     DATCZV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74710"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VIW27_06155"
     CDS             <1..797
                     /locus_tag="VIW27_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5739289.1"
                     /translation="EKTLWGFSAQTYRDFEIIVADDGSGPETRQVIESMQSQTDIPIR
                     HLWQEDDGFQKCRILNKAIVASRGEYLIFTDGDCIPHPDFVRNHVELAADNCLLSGGY
                     FKLPLSVSRAITREDIVSGNCTRPGWLLRHGVPFTPKLTKLYSHRFWGAILDALTITR
                     ATWNGHSSSTWKKHVLAVNGFDERMQYGGQDREFGERLVNLGLSTRQVRYRCSCVHLD
                     HGRGYASPESIARNRQIRRETRDKRITRTAYGIVPGEQQIAEAGRA"
     gene            794..1159
                     /locus_tag="VIW27_06160"
     CDS             794..1159
                     /locus_tag="VIW27_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY5739290.1"
                     /translation="MSGESLDRQQVAALRSALEERRSELEDQLRIGGDAARPVSLDQQ
                     SVGRVSRVDAIQQQQMALAGREQAELLLRQITQALQRIDDGVYGICLHCEQPIAAARL
                     VAQPQVSLCVNCQAASERD"
     gene            complement(1173..>2062)
                     /locus_tag="VIW27_06165"
     CDS             complement(1173..>2062)
                     /locus_tag="VIW27_06165"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5739291.1"
                     /translation="PRAAVSPRSGTPEASGRVLSLDKLDEFDDVLGAIEQACGMQNIP
                     TTSMISEYGAGQFEVNLQHQDDPLLAADHAALLRRAVQGVSRARGFDASFMSKPFADQ
                     SGSGLHLHLSLIDDEGNNIFDPRQPHGDRYLAQAVAGLQATMVEAMAIFAPNLNVYRR
                     FKPDEFVPVTTDWGDNNRSVAFRIPPSDAANRRIEHRVSGAEANPYLVVAAVLAGIHY
                     GLSAKLEPGGKHTGNAGAEVDSRLPLTLWQALAAIRDAEILPAYLGADYLQIYADVKQ
                     AEFQAFMADIHPREYEWYL"
BASE COUNT          370 a          680 c          664 g          348 t
ORIGIN      
        1 tggaaaaaac cctgtgggga ttctccgcgc agacctaccg cgatttcgaa atcatcgtcg
       61 ccgatgacgg ctccggtccc gaaacccgcc aggtgatcga atccatgcag tcgcaaacag
      121 atattccgat tcggcacctc tggcaggagg acgacggctt tcagaaatgc cgcattttga
      181 acaaagcgat cgtcgccagt cgcggcgaat acctgatatt caccgatggg gactgcattc
      241 cgcatccgga tttcgtgcgc aatcacgtcg agctggcggc tgataactgc ctgctgtcgg
      301 gcggctactt caagctgccg ctgtcggtat cgcgcgcgat cacgcgcgag gatattgtga
      361 gcggcaattg cacccggcca ggctggctgc tgcgtcacgg cgtgccgttc acaccgaagc
      421 tcaccaagct ctactcgcac cgcttctggg gcgcgatcct ggacgcgctg accatcaccc
      481 gcgctacctg gaacggacac agttcgtcga cctggaaaaa acacgtgctc gcggtcaatg
      541 gtttcgacga gcgcatgcaa tacggcggac aggaccgcga gttcggcgag cgcctcgtca
      601 acctcggcct gtcgacgcgg caggtgcgct accgctgcag ctgcgtgcat ctcgaccacg
      661 gccgcggtta cgcgagcccg gaatcgatcg cccgcaaccg gcaaatccgc cgcgagaccc
      721 gcgacaagcg catcacgcga accgcttacg gcatcgtccc cggtgagcaa cagatcgcgg
      781 aggccggtcg agcatgagcg gcgagagcct ggatcggcag caagtagccg ctctgcggag
      841 cgcgctcgag gagcggcgaa gcgagctcga ggaccagctg cgcatcggcg gtgacgccgc
      901 gcggccggtg tcgctggatc agcaatcggt cggcagggta tcgcgcgtcg acgccatcca
      961 gcaacagcag atggcgctcg ccggccgtga gcaggcggag ttattgctgc gacagatcac
     1021 gcaggccctg cagcgcatcg acgacggtgt ctacggtatc tgcctgcact gcgaacaacc
     1081 gatcgccgct gcccgcctcg tggcccagcc ccaggtctca ctgtgcgtga attgccaggc
     1141 cgccagcgag cgcgactagc cgcggccgtc gactacaggt accactcgta ttcgcgcgga
     1201 tggatgtcgg ccatgaaggc ctggaactcg gcctgcttga cgtcggcgta aatctgcagg
     1261 tagtccgcgc ccaggtaagc tggcagaatt tcggcgtcgc gaatcgcggc cagcgcctgc
     1321 cacagtgtca gcggcagtct cgagtccacc tcggcgccgg cgttgccggt atgcttgccg
     1381 cccggctcca gtttcgccga caggccgtaa tgaatgccgg cgagcaccgc ggcaaccacc
     1441 aggtacggat tggcctcggc accggaaacc cggtgctcga tgcgccggtt ggcggcatcg
     1501 ctcggcggaa tccgaaacgc gaccgagcgg ttgttgtcgc cccagtcggt ggtcaccggc
     1561 acgaactcgt cgggcttgaa tcgccggtag acgttcaggt tgggagcgaa gatcgccatg
     1621 gcttccacca tcgtcgcctg cagaccggca accgcctgcg ccaggtagcg atccccgtgc
     1681 ggctgccgcg gatcgaagat attgtttccc tcgtcgtcga tcaggctcag gtgcaggtgc
     1741 agcccgctgc ccgactggtc ggcgaacggc ttggacatga agctggcatc gaagccgcgt
     1801 gcgcggctga cgccctgtac cgcgcgccgc aacagcgccg cgtgatcggc ggccagcagc
     1861 gggtcatcct ggtgctgcag gttgacttcg aactgcccgg cgccgtactc gctgatcatc
     1921 gacgtggtcg gaatattctg cattccacag gcttgctcga tggcgccgag cacgtcgtcg
     1981 aactcgtcca gcttgtcgag cgacagcact ctgccactgg cctcgggcgt accgctgcgc
     2041 ggcgagaccg cggcgcgcgg cg
//
LOCUS       DATCZV010000276         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_340020, whole genome shotgun sequence.
ACCESSION   DATCZV010000276 DATCZV010000000
VERSION     DATCZV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340020"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /gene="phnY"
                     /locus_tag="VIW27_06170"
     CDS             <1..1111
                     /gene="phnY"
                     /locus_tag="VIW27_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEY5739292.1"
                     /translation="YHLAGQLVINDDSEVFSCDLTPHGKQRKIFTQREPLLGVISAIT
                     PFNHPLNMISHKIAPAIATNNRLVGKPTEQTPLTALLLADICYEAGLPPQMLSIVTGL
                     PADMGDEMITNPHIDLVTFTGSVPVGKYIAEKAGYRRTVLELGGNSELIVMDDADLDK
                     AAELAVAGGTKNSGQRCTAVKRVLAHDAIGDEFAALVAEKAAKLKYGDPMDPDTDVGT
                     VINEAAAKLFEARVADAIDKGAKLLYGHVREGAVYSPTVLDHVPFDCELVHEETFGTP
                     IPIIRIRDVDHAIEVGNSTDFGLSAGVCTNRMDDIIRFINELETGTVNIWEVPGYRIE
                     MSPFGGIKDSGLGYKEGVIEAMKSYTNVKTFSLPW"
     gene            complement(1188..1583)
                     /locus_tag="VIW27_06175"
     CDS             complement(1188..1583)
                     /locus_tag="VIW27_06175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5739293.1"
                     /translation="MPRKTLTPQQLDFQPRPTYPYSPGTRGGGMVYTSGQVAWGSDGN
                     VVGHGDIEAQTRQTLANVRAVLNEGGADWDDVLKCNVYLKDMNDFQRMNEIFMEIFPD
                     NPPARTTVQTPLAEATMLIEIEAVAWTGD"
     gene            complement(2048..>2635)
                     /locus_tag="VIW27_06180"
     CDS             complement(2048..>2635)
                     /locus_tag="VIW27_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5739294.1"
                     /translation="GSYFTNLQQGVSLRVEDHYTRAASVGGLGAAKTAANYAASLLPG
                     QQSRQQGFDQVLWLDGNEHRFIEEVGAMNIFFRIGDQVVTPPLGGSILPGVVRDSTLT
                     LLREWQLPVDERRITIDELLDAFRAGRVTEIFGAGTAAVICPVRCIGYQGEVFEVQGA
                     MPGELTTRLYDEITGIQYGKLPDRHGWTVRVPLPA"
BASE COUNT          509 a          848 c          806 g          472 t
ORIGIN      
        1 ctaccacctc gccgggcaac tggtgatcaa cgacgacagc gaagtgtttt cctgcgacct
       61 gacgccgcac ggcaagcagc gcaagatctt cacccagcgc gaaccgctgc tgggggtgat
      121 ctccgcgatc acgccgttca accatccgct gaacatgatt tcgcacaaga tcgcgccggc
      181 gatcgccacc aacaaccggc tggtcggcaa gcccaccgag cagaccccgc tgaccgcgct
      241 gctgctcgcc gatatctgct acgaagccgg gctgccgccg caaatgctgt ccatcgtcac
      301 cggcctgccc gcggacatgg gcgacgaaat gatcaccaac ccgcacatcg acctggtgac
      361 cttcaccggc agcgtgccgg tcggcaagta catcgccgaa aaggccggtt accggcgcac
      421 cgtgctcgaa ctcggcggca actccgaact gatcgtcatg gacgacgccg acctcgacaa
      481 ggccgccgag ctcgcggtcg ccggcggtac caagaactcg gggcagcgct gcaccgcggt
      541 caagcgcgtg cttgcgcatg atgcgatcgg cgacgagttt gccgcgctgg tcgcggagaa
      601 ggcggcgaag ctgaaatacg gcgacccgat ggaccccgac accgacgtcg gcacggtgat
      661 caacgaggca gccgcgaaac tgttcgaggc gcgcgtcgcc gacgccatcg acaagggcgc
      721 gaagctgctc tacggtcatg tgcgcgaggg tgccgtgtac tcgccgacgg tgctcgacca
      781 cgtgccgttc gactgcgaac tggtgcacga ggagaccttc ggcacgccga tcccgatcat
      841 ccgtattcgc gacgtcgacc acgccatcga ggtcggcaat tcgaccgatt tcggattatc
      901 ggccggtgtc tgcaccaacc gcatggacga catcatccgc ttcatcaacg agctcgaaac
      961 cggcaccgtc aacatctggg aggtgcccgg ctaccgcatc gagatgtcgc cgttcggcgg
     1021 catcaaggac tccgggctcg gctacaagga aggcgtgatc gaggcgatga agtcctacac
     1081 caacgtcaag accttctccc ttccctggta acatttaatt tgggtcagat ccaaatttcg
     1141 gtgtgtgccg ctttaatttg gatctgaccc aaattacgga aagggggtca gtcgccggtc
     1201 caggcgaccg cttcgatttc gatcagcatg gtggcctcgg ccagcggggt ttgcacggtg
     1261 gtgcgcgccg gcgggttgtc ggggaaaatt tccatgaaga tctcgttcat tcgctgaaag
     1321 tcgttcatat ccttgaggta aacattgcat ttgaggacgt cgtcccagtc ggcgccgccc
     1381 tcgttgagca ccgcgcgcac gttggcgagt gtctgccggg tctgcgcctc gatgtcaccg
     1441 tggccgacga cattgccgtc gctgccccag gcaacctggc cggaggtata aaccatacca
     1501 ccgccacgag tacccggcga atagggataa gtcgggcgcg gctgaaaatc cagctgctga
     1561 ggggtcaggg ttttgcgcgg catgaggctt ccttcaagta ggtcgttggc agccgtttta
     1621 cgccaaaccg cggcggattt ccagctggcg aggcatttcg cgggctgtgc cgaaacaccg
     1681 taattcaccc taccgaagcg ttgcgtatca aagcggtacc gaaatagccg cgggcagctt
     1741 cgcctcgtcg tcgctactgc tgctgttcga gcgccttgcg ctgggcctcg gccgcccgct
     1801 cgacgagcgg atctacctca cgggcacggc tgattggagc gtgctgtgaa gttccggccg
     1861 ttgccttcga gtcagcactc gatccaaaag ggtacccttt gaagatcagc agggacagga
     1921 gcagaaccgc agcaagcatg attaccattc gcatgtcccg agtatagatc ggaaaagacc
     1981 gctcgcagac gccgccgata gattcccggc gaatgcctac gggggccttt gcctgcgcga
     2041 tatcgaatca ggcgggcagc ggcacgcgca cggtccagcc gtggcggtcg ggcagtttgc
     2101 cgtactggat accggtaatt tcgtcgtaca gccgcgtggt cagctctccg ggcatcgcgc
     2161 cttgcacctc gaaaacctcc ccctggtacc cgatgcagcg caccgggcag attaccgccg
     2221 cggtaccggc accgaaaatt tccgtcaccc tgccggcgcg aaacgcgtcc agcaattcgt
     2281 cgatcgtgat gcgccgctcg tcgaccggca gttgccattc gcgcagcagc gtgagcgtgg
     2341 agtcgcgcac cacccccggc aggatcgaac cgccgagcgg cggcgtgacg acctggtcac
     2401 cgatgcggaa gaaaatattc atcgcaccga cttcctcgat gaaacgatgc tcgttgccgt
     2461 cgagccacag cacctggtcg aatccctgct ggcggctctg ctggcccggc aacaggctgg
     2521 cggcgtaatt ggccgcggtc ttggcggcgc ccagtccgcc gacgctggcg gcacgcgtgt
     2581 agtggtcctc gacacgcagc gatacgccct gctgcaggtt ggtgaaataa ctgcc
//
LOCUS       DATCZV010000277         3055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_234088, whole genome shotgun sequence.
ACCESSION   DATCZV010000277 DATCZV010000000
VERSION     DATCZV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234088"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1226)
                     /locus_tag="VIW27_06185"
     CDS             complement(72..1226)
                     /locus_tag="VIW27_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009405324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HEY5739295.1"
                     /translation="MRQASLTPAEFADIKGGYDDAAVRSHSIDTRCYLESRFLRIERE
                     QIFQRSWQFVCHEEKLRDPGSYVALDIEGQSVVALRNLDGELRAFYNVCKHRGHELLF
                     GEGRTRRITCPYHAWTYDLDGGLVNAPRSEHIENFERKSICLDAVQIETFCHLVFVNL
                     DAGAAPLAEQSGALGSEVMSYAPDLGELTFAARLTYNIKANWKSVVDNFLECYHCPVA
                     HKGFSSMIDMDSYRVETRGIYSSHMAKARAGANSAYNIDNATVTDHAVWFLWPNMTLM
                     RYPGRGNFMVWRFYPLGPEETYEIFDFFFATAEPTDDEREALRFIDEVLQPEDIGLVE
                     SVQRGMHTPAFNSGRYVVDADGSGMSEHAVHHFHGLLLDAYARACELGSV"
     gene            1368..1805
                     /locus_tag="VIW27_06190"
     CDS             1368..1805
                     /locus_tag="VIW27_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5739296.1"
                     /translation="MRPASSRGEAQAPRLLRPIGYQVPAKLREAILLMEGNIEEPQAQ
                     PELASYLELAVRQVQRIFRQYVGVTPIRHYLNLRLDRGRGLVTQTGMPIMEIAAPCGF
                     SRVEQFSRADVKRDQNAPIRDRIEGRIRFRFRDFTDHPGCRSV"
     gene            complement(1822..3027)
                     /locus_tag="VIW27_06195"
     CDS             complement(1822..3027)
                     /locus_tag="VIW27_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5739297.1"
                     /translation="MPTADSNTITPCSTTAIFDWLFTDGRRIESTSGFLRELGHRIND
                     NGGDVDRLMLSLMTLNPLIVGTADFWFRSTDTTNTVKASHNVRISERYIGSPLQAIYE
                     TRKRVRRRLDDLPEDAHRAYTELAEDGYTDYLALPVMFGERTDPGAAIVFCTRRPGGF
                     SDADIESYRRIRDYLAPVLEVHALRHQSRSLMNTYVGPRTSEKVLAGMVKRGDADIIN
                     AALWFSDLRNFTQVTETCASRDVLQMLNDYFEFIAAAVGAHGGEILRFIGDAMLIVFP
                     IGEGMSCEAACRAALESALDAQSTIATLNFQRRRHGQAQIEFGVGLNIGEVVYGNVGA
                     PDRLDFTVMGPTVNRTARLESLTKELDRDVLFSKEFSEYIDAPTEFLGDFAMKGIDVA
                     QPVYALLPE"
BASE COUNT          560 a          900 c         1004 g          591 t
ORIGIN      
        1 aacagcgcgg cccagctcgc gccgatgaca ccggcgccga gcgcgcagat ggtctcctgc
       61 ctgcgcattg gtcaaacgct cccgagttcg caggccctgg cgtaggcgtc gagcaacagg
      121 ccatgaaaat gatgtaccgc gtgctcgctc atgccgctgc catcggcatc gaccacgtag
      181 cgcccggaat tgaaggccgg ggtgtgcatg ccgcgctgca cgctctcgac gaggccgata
      241 tcctcgggct gcaacacctc gtcgataaag cgcagcgcct cgcgctcgtc gtcggtcggc
      301 tcggcggttg cgaaaaagaa atcgaatatc tcgtaagtct cttcggggcc gagcgggtaa
      361 aaacgccaga ccatgaaatt gccgcgtccc gggtagcgca tcagcgtcat attgggccac
      421 aggaaccaga ccgcatgatc ggtcaccgtg gcgttgtcga tgttgtaggc actgttggca
      481 cccgcgcgcg cctttgccat gtgactcgag tagatgcccc gggtctcgac ccggtagcta
      541 tccatgtcga tcatcgagct gaagcccttg tgcgcgaccg ggcagtggta gcactcgagg
      601 aagttgtcga ccaccgattt ccagttggcc ttgatgttgt aggtcaggcg cgcggcgaag
      661 gtcagctcac cgaggtccgg tgcataagac atgacttcac tgccgagcgc accgctctgc
      721 tcggcaaggg gtgccgcgcc ggcatcgaga ttgacgaata ccaggtggca aaaggtttcg
      781 atttgcaccg cgtcgaggca gatcgacttg cgttcgaagt tttcgatatg ttcggagcgt
      841 ggcgcgttga ccaggccgcc gtcgaggtcg taggtccagg cgtggtaggg gcaggtaatg
      901 cgtcgggtcc ggccttcgcc aaacagtaac tcgtggccgc gatgcttgca gacgttgtag
      961 aaggcccgca gctcgccgtc gaggttgcgc agcgcgacca cgctctgccc ctcgatatcg
     1021 agcgccacgt agctgcccgg gtcgcgcagt ttttcctcgt ggcagacgaa ttgccagctg
     1081 cgctgaaaga tttgctcacg ctcgatccgc aggaaacggg attcgaggta gcagcgggtg
     1141 tcgatcgaat gcgaacgcac ggccgcgtcg tcgtaaccgc ccttgatatc agcgaactcg
     1201 gccggggtga gactggcttg gcgcatggcg atcctccgca ataagtcgcg ctattagagc
     1261 gtaaacatag caatagcgac aggcaaaaaa ttgactgatt acgacatttg tccttgaaaa
     1321 acttcccgtt tcgcgcgcaa tctccgtttt cagcatgatc atttcagatg cgacctgcat
     1381 ccagccgcgg cgaggcgcag gcaccgcggc tattgcggcc gatcggctac caggtgccgg
     1441 cgaagctgcg cgaggcgatc ctgctgatgg aaggcaatat cgaggagccg caggcgcagc
     1501 cggagctggc cagttacctg gagctggcgg tgcgccaggt acagcggatc ttccgccagt
     1561 atgtcggcgt cacgccgata cgccattatc tgaatctgcg cctcgatcgc ggccgcggtc
     1621 tcgtcaccca gaccgggatg ccgatcatgg aaatcgccgc gccatgcggt ttttcgcgcg
     1681 tcgagcaatt cagtcgcgcc gacgtcaaac gtgaccagaa tgcgccgatt cgcgatcgta
     1741 tcgagggtcg tattcggttc cggtttcgcg atttcaccga ccatcccggg tgccgatcgg
     1801 tttgacagcg gccggccgcg attactcggg gagcaatgcg tataccggct gggcgacgtc
     1861 gatacccttc atcgcgaaat cgccgaggaa ttccgtcggc gcgtcgatgt attcggaaaa
     1921 ctccttcgaa aacagcacgt cacgatcgag ctccttggtc aggctttcga gtcgcgcggt
     1981 gcgattgacg gtcggcccca tgaccgtgaa gtcgagccgg tcgggagcgc cgacattgcc
     2041 gtagacgact tcgccgatat tgaggccgac gccgaactcg atctgtgcct ggccgtggcg
     2101 gcggcgctga aagtttagcg tcgcgatcgt gctctgcgcg tcgagcgcgg attcgagtgc
     2161 ggcccggcag gcggcctcgc agctcatgcc ttcgccgatc gggaacacga tcagcatcgc
     2221 atcgccgatg aagcgcagga tttcgccgcc gtgcgcgccg accgcggcgg cgatgaactc
     2281 gaagtagtcg ttgagcattt gcagcacgtc ccgcgaggca caggtctcgg tgacctgcgt
     2341 gaagttgcgc aggtcgctga accacaatgc ggcgttgatg atgtcggcat caccgcgctt
     2401 gaccatgccg gcgagaacct tttcgctggt gcgcgggccg acgtaggtgt tcatcagcga
     2461 gcgcgactgg tggcgcagcg cgtgcacttc cagcaccggt gcgaggtagt cgcggatgcg
     2521 gcgatagctc tcgatatccg cgtcgctgaa accgccgggc cgccgggtgc agaatacgat
     2581 cgccgcgccg ggatcggttc gctcgccgaa catgaccggc agtgcgaggt agtcggtgta
     2641 accgtcctcg gcgagttcgg tgtaggcacg gtgcgcatct tcggggaggt cgtcgaggcg
     2701 tctgcgcaca cgcttgcggg tttcatagat cgcctgcagc gggctgccga tgtagcgctc
     2761 ggagatgcgc acgttgtgcg atgccttgac ggtgttggtc gtatccgtgg acctgaacca
     2821 gaagtcggcg gtaccgacga tgagcgggtt cagcgtcatc agcgacagca tcaggcggtc
     2881 gacgtcaccg ccattgtcgt tgattcgatg gccgagttcg cgcaggaaac cgctggtact
     2941 ctcgatgcgg cggccatcgg tgaacagcca gtcgaagatg gctgtcgtgg agcagggggt
     3001 aatggtgttc gaatcggctg tcggcatggc tcagaagcgg gttgccaggg cggcc
//
LOCUS       DATCZV010000278         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_605111, whole genome shotgun sequence.
ACCESSION   DATCZV010000278 DATCZV010000000
VERSION     DATCZV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605111"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VIW27_06200"
     CDS             <1..1000
                     /locus_tag="VIW27_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY5739298.1"
                     /translation="AVADGESLGWNDIEAASMYQAERIPYLSEKILERLGITTADVIE
                     SIEAAIGGIAAGTAWSAPKAVIMPPDGRYMMAALAAMDDPSLLVVKTVVLNPRNSARG
                     LPQINGLVTVLDSSSGLPVAILDGNWVTAVRTAGLSAVAAKYMANRDASVAAFIGCGV
                     QARSHLQAFAEMFPLRRIRLFGRGQANIDRLCELAGKLGLIAELHDSPRAAIDGADLL
                     VTSVTFSPDLEPFLDADWLAPGCFAAITDLAAPWHRDSFAALDRIALDDLEQETALPN
                     KLVDLQYVSGDLLGLISGKFAGRDSVAQRTAFVFRGLAIGDLALTALALRQFNLAV"
     gene            complement(1086..1559)
                     /locus_tag="VIW27_06205"
     CDS             complement(1086..1559)
                     /locus_tag="VIW27_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY5739299.1"
                     /translation="MKKVSMLLISLVVIALIALFIIGRVSQNGQAPGITDGRLARCPD
                     TPNCVCSEYDGDQAHFIEPIELPAETAMTLARMRAAIIEQGGTIVSERADYLAATYTT
                     PLFGFVDDLEVRIEGDRGLAHVRSASRVGHSDLGANRKRVERLRELLRGSRADGD"
BASE COUNT          380 a          725 c          648 g          381 t
ORIGIN      
        1 cgccgtcgcg gacggcgaat ctcttggttg gaacgatatc gaggcagcat cgatgtacca
       61 ggcggaacgc attccctacc tctcggaaaa aatactcgag cggctcggca ttaccaccgc
      121 cgatgtgatc gagtcgatcg aagccgcgat cggtggcatc gccgcaggca ccgcctggag
      181 cgcgccgaag gcggtgatca tgccacccga cggccgctac atgatggcgg cgctcgcggc
      241 gatggacgat ccctcgttac tggtggtcaa aaccgtggtg ctgaacccgc gaaattcggc
      301 gcgcggattg ccgcagatca acggcctggt caccgtcctc gacagcagca gcggactgcc
      361 ggtcgcgatc ctcgacggca attgggtgac cgcggttcgc accgccggcc tcagcgcggt
      421 ggcggctaaa tacatggcca acagggacgc cagcgtggcc gccttcatcg gttgcggggt
      481 gcaggcacgc agccacctgc aggccttcgc ggagatgttc ccgctgcggc gcattcgcct
      541 gttcggccgc ggccaggcga atatcgaccg cctctgcgaa ctggccggca agctcggtct
      601 gatcgccgaa ctccacgact cgccgcgcgc cgcgatcgac ggcgccgatc tgctggtcac
      661 ctcggtcacc ttctcgcccg acctcgagcc gttcctcgac gccgactggc tggcgcccgg
      721 ctgcttcgcc gcaatcaccg acctggccgc gccctggcac cgcgacagct ttgccgcgct
      781 cgaccgcatc gcactcgacg atctcgagca ggaaaccgcc ctgcccaaca agctggtgga
      841 tttgcagtat gtcagcggcg acctgttggg gctgattagc ggcaagttcg cgggccgcga
      901 ctccgtcgct cagcgcaccg ccttcgtctt ccgcgggctc gcgatcggcg acctggcgct
      961 gaccgcactc gcgcttcgcc agttcaacct ggccgtctag ccggcaaacg cgccgctggc
     1021 cgcggttccc gttacgacaa cctcgaatta tcgacaccgc cccggagtga gaattcgcct
     1081 gccactcagt cgccgtccgc gcggctgccg cgcagcaatt cccgcagccg ctcgacgcgc
     1141 ttgcggttgg caccgaggtc gctgtgcccg acccgcgacg cggaacgcac gtgagccagg
     1201 cctcggtccc cttcgatgcg aacctcgaga tcgtcgacga aaccgaaaag cggcgtggtg
     1261 taggtcgcgg cgaggtaatc cgcgcgttca gacacaatcg tcccgccctg ctcgatgatc
     1321 gccgcccgca tccgggcgag cgtcatcgct gtctctgccg gcaattcgat cggctctatg
     1381 aaatgcgctt gatcgccatc gtattcgctg caaacgcagt tcggcgtatc gggacaacgc
     1441 gccagccggc catcggtgat gccgggagcc tgaccattct gcgaaacccg gccgatgatg
     1501 aaaagcgcga tcagagcaat caccaccagg ctgattaaaa gcatagagac ctttttcata
     1561 ctcatttcac gaggctatac gagtggctac gcatcgccga cccattcgga tttctcgcac
     1621 ggcgagaatc gagctcgaag tataaccgtt tatcgtcgcc gggttcggcg cttcggcaag
     1681 cccgcgcgcg ccttccagtc gcgaacgcca ggcgctgatc cgcggaccaa agcgacccca
     1741 cgggttcgac ccggccgcaa acgccgccgt cggtccagcc gcggttgcgc atcgagtggc
     1801 gggatcggtg ctgcggtgag cgccggggtt acggtaatcc agaagattgt ccacggtcgc
     1861 tcgcggttcg ccccggttag agttcgcgaa ttcgattatc cactgacaca caggcatttt
     1921 acaaaatgtc cgtaatcggc tcgctcatcc ctattggatc gatgatcggc cacgccgacc
     1981 agcgccatgc gctgccacgt tgttcgctgc tggccgaaaa tacgggatcg cgaactgcgg
     2041 tcgagcacca tgcaacagct cggcaatgcc tcgtttcgct tgtccggtcg gggtctctct
     2101 gcgaagctag tcgcgctaat acaaagcgga gggt
//
LOCUS       DATCZV010000279         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_181796, whole genome shotgun sequence.
ACCESSION   DATCZV010000279 DATCZV010000000
VERSION     DATCZV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181796"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIW27_06210"
     CDS             complement(<1..214)
                     /locus_tag="VIW27_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739300.1"
                     /translation="MPTAKPSIDTQISWVYTEDLESSHRFYSRILDFECVRNEGMARI
                     YRTTPGACIGVCKAHDDRIVEPRGGMI"
     gene            333..1520
                     /locus_tag="VIW27_06215"
     CDS             333..1520
                     /locus_tag="VIW27_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739301.1"
                     /translation="MQRPAYILPVIIFSQFTGTSLWFSGNAVLLDLQRDWGLSAHATG
                     YTTSAVQLGFVSGTLLFAFFVIADRFSPRLVFFCCSLAGALANAALLIAPEGLAWLLL
                     LRFATGFFLAGIYPVGMRIAAGWYAEGLGRALGYLVGALILGTAFPHLLRASGTEFPW
                     QQVLGWVSLLAAGGGLAMLLLVPDGPHLPHGTRFDPRALLIVFRSAKFRASAFGYFGH
                     MWELYTLWAFIPTLLIAYAAAQDIDLNVSLWSFAAIAVGALACAGGGILSTRIGSAPV
                     AASLLAISGMCCLLSPFLFTASPPLFLAFLLLWGLSVSSDSPQFSALNAANAPREFVG
                     SALTIVNSIGFLITVVSIQLADLLLTLIETRYLFWLLVPGPVLGLLAFRPLLGEHRAA
                     ARQ"
     gene            1729..2418
                     /locus_tag="VIW27_06220"
     CDS             1729..2418
                     /locus_tag="VIW27_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY5739302.1"
                     /translation="MTTAQSVMHGIRTSADYFLSTFIFGIMFGITASAAGLENWQALL
                     MSATVFTASGQFAALEFWQAPLPLATIALSVFLVSTRNILLGMAMTYHFDGHSLGRRI
                     AWLFLLNDPGVVNSFRQDPQVDRLGYVTGYGLSMMTSWLISTFLGFNGAQLFARLDLG
                     AVGFAGPLVLATMMMLFFKSAKTPPTPWLLSGAVALVLVEIGSPDYAVLLGSVLIGLV
                     AAIWQARRTHG"
BASE COUNT          360 a          835 c          764 g          552 t
ORIGIN      
        1 aaatcatgcc accgcgcggc tcgacgatcc ggtcgtcgtg tgccttgcaa accccgatac
       61 aggcgcccgg cgtcgtccgg tagattcgcg ccataccctc gttgcggacg cattcgaaat
      121 cgaggattcg gctatagaaa cggtgcgaag attcgagatc ttcggtgtag acccagctga
      181 tctgggtgtc gatcgatggc ttcgcggtag gcatgttcac aggctcgtca atgcgccgat
      241 tatcggccgc aacacggccg cgatcaacag catcagccgc gccgcccgcg ccatcgctgg
      301 cccgattcga gcactgctgg tacatttggc cgatgcaacg ccccgcttac attttgccgg
      361 tgatcatttt ctcgcagttc accggaacct cgctatggtt ctccggcaac gcggtgctac
      421 tcgacctgca acgcgactgg gggctgtcgg cacatgccac cggttacacg acctcggcgg
      481 tacaactcgg cttcgtcagc ggcacgctac tgttcgcgtt tttcgtcatc gccgatcgct
      541 tctcgccgcg gctggtgttt ttctgctgtt cgctggccgg cgcactggcc aacgccgcgc
      601 tgctgatcgc ccccgagggc ctggcgtggt tactgctgct gcgcttcgcg accggttttt
      661 ttctcgccgg catctatccc gtgggcatgc gaatcgccgc cggctggtat gcagaaggcc
      721 tcggccgcgc gctcggttac ctggtcggcg cgctgatcct cggcaccgcg tttccgcacc
      781 tgctgcgggc cagcggtacc gaattcccgt ggcagcaggt actcggctgg gtttcgctac
      841 tcgccgccgg cggcggtctg gcgatgctac tgctggtccc ggacggtcca cacctgccgc
      901 acggcacgcg cttcgatccg cgggcgctgc tcatcgtttt ccgttccgcg aaattccgtg
      961 cctcggcctt cggttacttc gggcacatgt gggaactgta caccctgtgg gccttcatcc
     1021 cgaccctgct gatcgcctac gcggcagcac aggatataga cctcaacgtg tcgctgtgga
     1081 gtttcgcggc gatcgcggtc ggcgcgcttg cctgtgccgg cggcggcatc ctgtccaccc
     1141 gcatcggcag cgcgccggtg gccgcctcgc tgctggcaat ttcggggatg tgctgcctgc
     1201 tgtcgccgtt tctgtttact gccagcccgc cgctgttcct cgccttcctg ctgctgtggg
     1261 gcctttcggt gtccagcgat tcaccgcagt tctcggcgct gaacgcggcc aatgcgccgc
     1321 gcgagttcgt cggctcggcg ctgaccatcg tcaattcgat cggctttttg atcaccgtgg
     1381 tcagtattca gctcgccgat ctattgctga cattaatcga aactcgctac ctgttctggc
     1441 tgctggtgcc cgggccggta ctcggcctgc tggcgtttcg cccgctgctt ggcgagcacc
     1501 gggcggcggc ccggcaataa tcgcgaagtg ctcgcggcag ctgtgctatg gtcgcgcctg
     1561 cgtacgataa tcctgccaac ggaaaccgcg cttgactgac aaaaccgcga accgtcgccc
     1621 tcgctcgcag ccgccgatac aggtcagctg catcccgccg ccgggaatcg gcatcgcctg
     1681 cggaccggcg tcgatcatgc tgttcgaaaa aagcgcacgg caatccgcat gacgaccgcg
     1741 caaagcgtta tgcacgggat tcgcacttcc gcggattatt tcctgtcgac cttcatcttc
     1801 ggcatcatgt tcggcatcac cgccagcgcc gccggcctgg aaaactggca ggcattgctg
     1861 atgagcgcaa cggttttcac cgcttccggg cagtttgccg cgctcgagtt ctggcaggcg
     1921 ccgctgccgc tggccaccat tgcattgtcg gttttcctgg tcagcacacg caacatcctg
     1981 ctcggcatgg cgatgactta tcatttcgac ggccacagtc tcggcaggcg catcgcctgg
     2041 ctgttcctgc tcaacgatcc gggcgtggtc aacagttttc gccaggaccc gcaggtcgat
     2101 cgcctcggct acgttaccgg ctacggcctg tcgatgatga ccagctggct gatttcgacg
     2161 tttctcggct tcaacggtgc gcaattattt gcgcgcctcg atctcggcgc ggtcggcttt
     2221 gccggcccgc tggtgctggc gacgatgatg atgctgttct tcaagtcggc gaagacgccg
     2281 ccgacgccgt ggctgctgtc cggcgcggtg gcgctggtgc tggtcgaaat cggcagcccc
     2341 gattacgcgg tgctactggg ctcggtactg atcggtctgg tggccgcgat ctggcaggcg
     2401 aggcgcactc atggctgagg ccctgaccgc gatcgcgatg atggcggcga tcgtctacct
     2461 gacccgcgcc accggctatt tcatcggcct gcagctgcgc cacattcgcg g
//
LOCUS       DATCZV010000280         5043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_22961, whole genome shotgun sequence.
ACCESSION   DATCZV010000280 DATCZV010000000
VERSION     DATCZV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22961"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2935)
                     /locus_tag="VIW27_06225"
     CDS             complement(<1..2935)
                     /locus_tag="VIW27_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY5739303.1"
                     /translation="MNTKQFINTMLVTASVFACQVEAAAPFNPELQPLGYIGPIELSK
                     TDLSKGAKAYRGWFENGAWQGDLIEYDVSKTGVLSTSIDLTDPSPKQGALPTNWSAHV
                     QFAANANTISHWDTGRKIITSTTGTNQLAFRWANLTDAQKKLLDMTAFKKKKATSDIV
                     DFLRGDRSKENPNGPLRMRFTVMGDVIHSHPEYVGIPSGTYTDESYITFKNANLSRSP
                     RVYVGANDGMMHAFDAETGNEVWAYVPSMVVHKMSKLAGIPYAHTYFVDGGVTAQDAY
                     FDNAWHTILIGSMGAGAKGLFALDVTSPDLSSENLAGGSDKKILWELDDNDDNLGYIF
                     EASTIAQMNDGKFYAVNGNGVSSAKGVAMLYLVEIKNGNVKKLTTASGSVNEPNGLSA
                     PALVDTDGDGIVDVAWAGDINGDMWRFDLSDTNAGNWKVDYKLFDGTDAQPITTAPDI
                     ANHPQIGHLVLYGTGRLYTDADIMDKRIQSLYGLWDTGTAPSGGELRLAQLLSDDTPY
                     KGGGFNEWVRTFTTVDPLDYSSYQGWKVDLPAGERVVTGGQLRGGRLKVTVTDPDGYF
                     NWLHETTFDEGGVEDDSIFDLNRDNLLNAFDRVDGNTDGDLDDARDIPMGWERERGNM
                     SQATIGNLGPGVDTLFLNFLNPPLVPPACEGICAGGLSGGHMDVDTDAANMDGWGGGT
                     NGHVHEYDDKFDVTFIDFLNIDRGGVGNLLPVYDAVDGDEEFIVLVANADMSIGGNIT
                     INNKTYNVTEYQRMIHMALQKWDGVGALTAPAEYGGSSLITTLDQIDLDGGGLGIEFN
                     SRAIIGGGLHPTQTGCVNKAPSVTNGRWRNGALTAHLVRKSYFNSLGGANALDRVIVQ
                     YPSDLKEVVVLADGTEVELSADIDGNGTIDGSSPAYETFGGLISMGDNTNSFLYEATV
                     FWHFGDRTKEILGIKGVCYGEDRWEEAFQVEIGNVAPEVYQDLLTEAKFASFEELTLR
                     VEELKG"
     gene            complement(2981..3514)
                     /locus_tag="VIW27_06230"
     CDS             complement(2981..3514)
                     /locus_tag="VIW27_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY5739304.1"
                     /translation="MKAQNLKYLPTASRQRGVSLIVAMVMLIVLTLIGVSSMNTAIVE
                     LKMAGSMQQQSVALNRADELLRVGEQDIDAIVDAPAAFNFTTAGDGYYVAADNIDVFD
                     LSWSGLNPIEGATENDVYVTEYLGPKAIPGESVKVSTDGRIVGGAVHTFRITSRSATG
                     KGALRLAQSLYVTILPP"
     gene            complement(3527..4465)
                     /locus_tag="VIW27_06235"
     CDS             complement(3527..4465)
                     /locus_tag="VIW27_06235"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY5739305.1"
                     /translation="MIRIATIDSKTLTRQSGLSMVELMVAMALGLFLVLALVQILING
                     KESFGSANHLSRLQETGRIATGLMITDLKRAGYMGGNSDIPNIFGSTGQLNPDLTCVT
                     GNSTWVRMVSQPVEGVDDSNAGYTCIPDATYLRGDVVTVRYTAPFVAVGALDNNRLYL
                     RSSLFEGKIFVGSAEAQAVNQVMDTPQNVRELLAYSYFVGDSGRTCNGATVPSLFRVR
                     VDEIGQPIVEELLPGIDNLQARYSDGQRYYDADNVPDWDAVIAARIWLLVRAECAETG
                     FTDGNTYNMGDQSITPNDSFRRQLYSSVVMIRNFRN"
     gene            complement(4492..4995)
                     /gene="pilV"
                     /locus_tag="VIW27_06240"
     CDS             complement(4492..4995)
                     /gene="pilV"
                     /locus_tag="VIW27_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY5739306.1"
                     /translation="MPVKINHADRQTIRRRQRGDTMIEVLVTIIVIAVGVLGAAALQV
                     TTLKNLSSSHSATVAALVAEDMTERMRANPVGAANYVHSARPVNPPNCTSQGCTPAEL
                     AAYDIGSWWNQMTALLPSASGTVTNAGGTFTVTVRWDEDRSGSTGTNCPPESADDLEC
                     YRFNVTM"
BASE COUNT          965 a         1507 c         1469 g         1102 t
ORIGIN      
        1 agcccttgag ttcctcgacc cgtagcgtca gttcctcgaa agatgcgaat ttcgcttccg
       61 tgagcagatc ctgataaacc tccggggcaa cattaccgat ttcgacctga aaagcttctt
      121 cccagcgatc ctcgccgtaa cagacaccct tgatcccgag aatctccttg gtccggtcgc
      181 cgaagtgcca gaatactgtg gcctcgtaaa ggaacgagtt tgtgttatcg cccatggaaa
      241 tcagaccgcc gaaagtttcg taggccgggg agctaccgtc gatagtgcca ttgccatcga
      301 tgtccgcgct caactcgact tcggtaccat cggccagcac caccacctct ttcaggtctg
      361 acgggtattg cactatcacc cgatccagcg cgttggcacc accgaggcta ttgaaataac
      421 tcttcctcac caggtgagcc gtcaatgcac cattacgcca gcggccattg gtgacactcg
      481 gggccttgtt gacgcagccg gtctgtgtcg ggtgcaggcc gccaccgatg atcgcccggc
      541 tattgaattc gattcccagt ccgccaccat caaggtcgat ctggtcgagg gtggtgatca
      601 acgagctgcc gccgtactcc gcgggagccg taagcgcacc cacaccatcc cacttctgca
      661 gcgccatgtg gatcatccgc tggtattcgg tcacgttata ggtcttattg ttgatcgtga
      721 tatttccgcc gatcgacatg tcagcgttgg caaccagcac gataaactcc tcgtcgccgt
      781 cgacggcatc atataccggc aacaagttac cgacaccacc gcgatcgatg ttgaggaaat
      841 cgataaaggt gacatcgaac ttgtcgtcgt actcatgtac gtggccattg gtgccaccgc
      901 cccagccgtc catattcgcg gcatccgtat cgacgtccat gtgaccaccg ctcaggccac
      961 cggcacaaat accctcgcac gcaggcggca ccagcggcgg gttcaaaaag ttcaggaaca
     1021 gtgtgtcgac accgggcccg aggttgccga tggtcgcctg cgacatattg ccgcgctcgc
     1081 gctcccagcc catcggtatg tctctggcgt cgtccaggtc accgtctgtg ttgccgtcga
     1141 cacggtcgaa ggcattcagc aggttgtcgc gattgaggtc gaaaatcgag tcgtcctcga
     1201 cgccgccctc atcgaaggtc gtctcgtgca gccagttgaa gtagccgtcg ggatcggtga
     1261 ccgtcacctt caggcggccg ccgcgcaact gcccccccgt caccacgcgc tcaccggcgg
     1321 gcaagtcgac tttccagccc tggtagctac tgtaatcgag cgggtcgacc gtggtaaaag
     1381 tacgtaccca ttcgttgaag ccaccgcctt tgtaaggggt atcgtccgat aacaattgcg
     1441 cgaggcgaag ctcgccgccg gaaggcgcgg tgccggtatc ccagaggccg tacagcgact
     1501 ggatccgctt gtccatgata tcggcatcgg tatacaggcg gccggtaccg tacagcacca
     1561 ggtgaccgat ttgcggatgg ttggcaatat cgggggcagt ggtgatgggc tgtgcgtcgg
     1621 tgccgtcgaa cagcttgtaa tcgactttcc agttgccggc gttggtgtcg ctcaggtcga
     1681 agcgccacat gtcaccattg atgtcgccgg cccaggcaac gtctacgatg ccgtcgccgt
     1741 cggtatcgac cagcgccggt gccgacagtc cattgggctc attgacagaa ccgctggcag
     1801 tggtgagttt tttcacattt ccattcttga tttccaccag gtacagcatc gcgacccctt
     1861 tggcgctgct gacaccgttg ccattgaccg cgtagaactt gccatcgttc atctgcgcga
     1921 tcgtcgaggc ttcgaagata tagcccaggt tgtcgtcgtt atcgtcgagt tcccagagga
     1981 tcttcttgtc gctgccaccg gcgaggttct ccgagctgag atccgggctg gttacgtcga
     2041 gcgcgaacag gcccttggcc ccggcaccca tgctgccgat cagtatcgtg tgccaggcat
     2101 tgtcgaaata ggcgtcctgc gcggtaacac cgccgtcgac gaaataagtg tgcgcgtagg
     2161 gaatacccgc cagcttggac atcttgtgga caaccatgga cggcacgtag gcccagacct
     2221 cgttaccggt ttcggcgtcg aaggcgtgca tcatgccgtc gttggctccg acgtaaaccc
     2281 gcggcgagcg gctcaggttg gcattcttga atgttatata gctctcatcc gtgtaagtac
     2341 cgctgggaat acccacgtat tcggggtgcg agtgaatcac gtcgcccatc accgtgaatc
     2401 gcatgcgcag tgggccattc gggttttcct tgctgcggtc gccgcgcaga aagtcgacaa
     2461 tatcactggt cgcctttttc tttttgaaag cggtcatgtc cagcagtttc ttttgcgcat
     2521 cggtcagatt cgcccagcga aacgcaagct ggttggtgcc ggtggtcgaa gtgatgattt
     2581 tcctgccggt atcccagtgg ctgatggtat tggcgttggc cgcaaactgc acgtgggcag
     2641 accagttggt aggaagggcg ccctgcttcg gcgagggatc cgtcaggtcg atactggtcg
     2701 acagcacacc cgttttgctg acgtcgtact cgatcaggtc gccctgccag gcaccgtttt
     2761 cgaaccagcc ccgataggcc ttggcaccct tgctgaggtc ggttttgctg agttcgatcg
     2821 ggccgatata gcccagcggc tgcagttcag gattgaacgg ggcggccgcc tcgacctggc
     2881 atgcaaatac cgatgcggtc accagcatgg tgttgataaa ttgtttggta ttcacggttt
     2941 gatctccata aaaatcgata gctctctgca gcaaccttgc ttacggcggc aatatcgtca
     3001 cgtagaggct ttgcgccagc cgcagagcac cttttccggt cgcgctgcgg gaggtaatgc
     3061 gaaaagtatg cacggcaccg ccgacgatac ggccgtcggt gctgaccttg accgactccc
     3121 cgggaatcgc cttcggaccc aggtactcgg tgacgtatac gtcgttttcg gtggcgccct
     3181 cgatcgggtt caggccggac cagctgagat cgaaaacatc gatgttgtcg gcggccacgt
     3241 agtagccgtc gccggcggtg gtaaaattga atgcggccgg ggcgtcgacg atcgcatcga
     3301 tatcctgttc gccgacgcgc agcaattcgt ccgcgcgatt gagcgcaacg ctttgctgct
     3361 gcatgctgcc ggccatcttc aattcgacga tggcggtatt catcgagctg acgccgatca
     3421 gcgtcaacac gatcagcatc accatcgcca cgatcagcga tacgcctcgt tggcgggaag
     3481 cggtgggcag gtatttcagg ttctgcgctt tcatctcgat tcgatcctag tttctgaaat
     3541 ttctgatcat cacgacgctg gaatacaact ggcgtcgaaa gctgtcgttg ggcgtgattg
     3601 actggtcgcc catgttgtag gtattgccgt cggtaaaccc ggtttcggcg cattcggcgc
     3661 gcaccagcag ccagattcgg gcggcgatga ccgcgtccca gtcaggaacg ttgtcggcgt
     3721 cgtagtaacg ctggccgtcg ctgtaacgtg cctgaaggtt gtcgataccg ggcagcaact
     3781 cctcgacgat cggctgaccg atctcgtcga ccctgacgcg aaacagcgat ggcaccgtgg
     3841 cgccgttgca ggtgcgcccg gagtcgccga cgaaataact gtaggcgagc agttcgcgca
     3901 cgttctgcgg tgtatccatg acctggttga cagcctgcgc ctcggcgctg ccgacaaaaa
     3961 tcttgccctc gaacagcgaa ctgcgcaggt acaggcggtt gttatcgagc gcaccgaccg
     4021 cgacgaatgg tgcggtgtag cgcaccgtga ccacgtcgcc gcgcagatag gtggcgtcgg
     4081 gaatgcaggt gtagccggca ttcgaatcgt cgacgccctc gacgggctga ctgaccatgc
     4141 gcacccaggt gctattgccg gtaacgcagg tcaagtcggg attcaactgg ccagtgctgc
     4201 cgaaaatatt cggtatatcg gagttaccgc ccatgtagcc ggcgcgcttc aggtcggtaa
     4261 tcatcaaccc ggtcgcgata cggccggttt cctgcagccg cgacagatga ttggcggagc
     4321 cgaaagattc cttgccattg atcagtatct gcaccagcgc gagcaccagg aacaggccga
     4381 gagccatggc caccatgagt tccaccatgc tcaggccgct ctgccgcgtc agtgtctttg
     4441 aatctattgt cgctattctg atcactattc ttatctcgct gctccacaaa cctacatggt
     4501 gacgttgaac cggtagcact cgaggtcgtc ggccgactcc ggcgggcaat tggttccggt
     4561 gctgccgctg cgatcttcat cccagcgcac cgtgacggtg aaggtgccgc cggcgttggt
     4621 aacagttcca ctggccgaag gtaacaacgc ggtcatctgg ttccaccagc tgccgatatc
     4681 gtaagccgcc agttccgccg gggtgcagcc ctggctggta caattgggcg gattgaccgg
     4741 cctggcactg tgcacatagt tggccgcccc gaccgggttg gcgcgcatgc gctcggtcat
     4801 atcttcggcc accagcgcgg ctacagtcgc gctatgcgaa ctgctcagat tcttcaacgt
     4861 ggtgacctgc aaagccgcgg cgccgagcac cccgacagcg atgacgatga tcgtgaccag
     4921 cacttcgatc atggtatccc cgcgctggcg gcgacggatg gtttggcgat cggcatggtt
     4981 aatctttaca ggcatgctgc ttgccccgta tccgatgctt tcaagacact gccgcggccg
     5041 gtt
//
LOCUS       DATCZV010000281         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_499199, whole genome shotgun sequence.
ACCESSION   DATCZV010000281 DATCZV010000000
VERSION     DATCZV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499199"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VIW27_06245"
     CDS             <1..274
                     /locus_tag="VIW27_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5739307.1"
                     /translation="EIFAIVKRIGPGGHFLGEQHTLDSKLFIFDSQHNNSYEQWDAEG
                     RLESEAVGVRKAQKWLQKYQPPPIDAALDAALREYIERRSREIPAG"
     gene            complement(329..895)
                     /locus_tag="VIW27_06250"
     CDS             complement(329..895)
                     /locus_tag="VIW27_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY5739308.1"
                     /translation="MSVGVPRLIFAALVALFTSAAFAGEADDDKAALKKLMQSDVVAS
                     FHKSAYGYAIFPNIGKGGIGIGGAHGKGRVYRNGKKLGDVSMTQLSIGLQLGGQAYRQ
                     VIYFEDERALKEFTSGSFEFGAQAEAIAITASAGAQAGTEGASASANETQAQAKYYKG
                     MIVFTMGKGGLMYQASIGGQQYKFTPSK"
     gene            1094..1690
                     /locus_tag="VIW27_06255"
     CDS             1094..1690
                     /locus_tag="VIW27_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5739309.1"
                     /translation="MEKIAQRPVGRPREFDENRVLEAAMNAFWNKGYEATSLAELCRV
                     TGLNKGSLYQAFGDKHSLFMRALTHYSEMEYREVMAVAVQHESPLARIRAIVRKICDD
                     ACGGRGCMINNSMVELAPHDPEVKAALQTFGEQRMQGLTGVIGAAQLVGEIRAELEPR
                     KLARQLMMTLAGAAAMSKGFLSNDETNETLAALIDSWT"
     gene            1751..>2505
                     /locus_tag="VIW27_06260"
     CDS             1751..>2505
                     /locus_tag="VIW27_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5739310.1"
                     /translation="MGTYFKAIDRFAAALTRRIISRPWLVVALSLALATAAGFGAGQL
                     EFSTNYRVFFSKANPELQAFENLQNTYTKNDNFVFVFEPRDGGAFSPATLAAVERLTE
                     AAWQIPYAIRVDSVTNFQHTHALGDELIVEDLVSDAASRSADFLADREAIALAEPLLR
                     DSLLTRDGGVTAVNVVLQYPQKALTEVPEAVAVARALKSEIETAFPSIDIHLTGVSML
                     NNAFAETGMNDAATLMPLMFAVIFALILVIVRS"
BASE COUNT          497 a          748 c          780 g          480 t
ORIGIN      
        1 ggaaatcttc gcgatcgtca agcgcatcgg ccccggcggt cattttctcg gcgaacagca
       61 tacgctcgac agcaagctgt tcatcttcga ctcgcagcac aacaattcct acgagcagtg
      121 ggacgccgag ggacggctgg agagcgaggc ggtcggcgtg cgcaaggcgc aaaagtggct
      181 gcaaaagtac cagccgccac cgatcgatgc ggcactcgac gcggcgctac gggaatacat
      241 cgagcgccgc agccgggaaa tcccggccgg ctgacgggca caaggcgcgc ggcgaattgg
      301 tctccgccgc gcccgggtca agccgggctt atttgctcgg cgtgaacttg tactgctggc
      361 cgccgatcga cgcctggtac atcaggccgc ccttgcccat cgtgaagacg atcatgccct
      421 tgtaatattt ggcctgggcc tgggtctcgt tggcactggc gctggcgccc tcggtgccgg
      481 cttgcgcgcc ggcgctggcg gtaatcgcga tggcttccgc ctgggcgccg aattcgaaac
      541 tgccgctggt gaactccttc aacgcacgct cgtcctcgaa gtaaatcacc tgccggtaag
      601 cctggccgcc gagctgcagg ccgatgctga gctgggtcat cgacacgtcg ccgagtttct
      661 tgccgttgcg gtataccctg cccttgccat gcgcgccgcc gatgccgatg ccgcccttgc
      721 cgatattggg aaatatcgcg taaccgtaag ccgacttatg gaagctggca accacatcgg
      781 attgcatcag ttttttcagc gccgccttgt cgtcgtcggc ttcgccggcg aacgcggcag
      841 aggtgaacag ggccacaagg gcagcgaaaa tcagcctggg tacgcctacg ctcattgggt
      901 cgtttcctga tgttcgaatt gaatgcaaat cggaattatc tggaaattcg agcgatccgc
      961 aagcgccgaa tacgccacgg gcgaatacgc gacccgcggg cggcgatatc gaacaatttt
     1021 tgaccgaggg gtttacaatt gtcaggtttt cggctagatt ctagaccgtt cggtaattaa
     1081 cctatacgag gcaatggaaa aaatcgcgca aagaccggtc ggacggccgc gggaattcga
     1141 cgaaaaccgg gttctcgagg cggcgatgaa cgcgttctgg aacaagggct acgaggccac
     1201 ttcgctggcg gaactgtgcc gcgtgacggg tctcaacaag ggcagtcttt accaggcttt
     1261 cggcgacaag cacagcctgt tcatgcgcgc gctgacgcat tattccgaga tggagtaccg
     1321 cgaggtgatg gcagtggcgg tgcagcacga gtcgccgctg gcgcgcattc gcgcgatcgt
     1381 gcgcaagatc tgtgacgatg cctgcggcgg caggggttgc atgataaaca attcgatggt
     1441 cgaactcgcg ccgcacgatc ccgaggtcaa ggccgcgctg caaacctttg gcgagcagcg
     1501 catgcaggga ttgaccgggg tgatcggcgc ggcgcagctg gtgggtgaga ttcgcgccga
     1561 actcgagccg agaaagctcg cgcggcaact gatgatgacg ctggccgggg cggcggcgat
     1621 gtcgaagggc tttctgtcga acgacgaaac caacgagaca ctcgcggcat tgatcgattc
     1681 ctggacctga gttttttttg actaattatt gaccgattgg tcatttaata gctaaacacg
     1741 gagatacgac atgggcacct acttcaaggc aatagaccgc ttcgcggcag cgctgacaag
     1801 gcgaatcatt tcacgaccct ggctggtcgt cgcgctgtcg ctggcgctcg ccacggcggc
     1861 cggtttcggc gccggccagc tcgagttttc gaccaattac cgggttttct tttccaaggc
     1921 caacccggag ctgcaggctt tcgaaaacct gcaaaacacc tacaccaaga acgacaactt
     1981 cgtgttcgtc ttcgaaccgc gcgacggcgg cgcgttcagc ccggcgacgc tggccgcggt
     2041 cgagcggctg accgaagcgg cctggcagat tccctatgcg ataagggtcg attcggtcac
     2101 caacttccag catacccacg cgctcggcga cgaactgatc gtcgaagacc tggtcagcga
     2161 tgccgcttcg cgttcggcgg actttctcgc cgatcgcgaa gcgatagcgc tcgccgagcc
     2221 gctgttgcgc gacagcctgc taacccgcga cggcggcgtc accgcggtca acgtggtgtt
     2281 gcaatacccg caaaaagcat tgaccgaggt acccgaggcc gtcgccgtgg cgcgcgcgct
     2341 gaagagcgaa atcgagaccg ccttcccgag catcgatatc cacctgaccg gcgtgtcgat
     2401 gctcaacaat gccttcgccg agaccggcat gaacgacgcg gcgacgctga tgccgctgat
     2461 gttcgcggtg atcttcgcgc tgatcctggt gatcgtgcgt tcgtt
//
LOCUS       DATCZV010000282         5614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_234783, whole genome shotgun sequence.
ACCESSION   DATCZV010000282 DATCZV010000000
VERSION     DATCZV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234783"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VIW27_06265"
     CDS             complement(<1..606)
                     /locus_tag="VIW27_06265"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5739311.1"
                     /translation="MGKLEQVLRDGHFALTAETSPPDAASAQAVVERVECLRGLADAV
                     NVTDGASARVHMAALAAAAVMASEGIEPVLQMTTRDRNRLALQGDVVGAAALGIRNVL
                     CLTGDKMAAGDQPAAAEVFEIDSGELMRQIRDMRDLGQFPTGRAIDPAPQLFLGAAEM
                     PAEPGEHWPAARLQAKIDNGTQFFQTQYCFEVDKVRRYCRAL"
     gene            complement(615..2027)
                     /locus_tag="VIW27_06270"
     CDS             complement(615..2027)
                     /locus_tag="VIW27_06270"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013463354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY5739312.1"
                     /translation="MQNLPGKAAADLPTSWRGLPIAQQPVYPDARRLADVERRLRALP
                     PLVFAQEIRDLRQNLAAVAGGRGFLLQGGDCAESFAGFNAGKIRDTFRVLLQMAVVLT
                     FAGRRPVTKIARTAGQFAKPRSVDTELVDGIELPSFRGDIVHAAEPTAAARVPDPERM
                     LQAYNQSASTLNLLRAFAQGGLADLHQVHRWNMSFVESSPGKERYLELASRIEDALGF
                     MEVCGITSATAPSIRETRLFTSHEALLLNYEEALSRIDSFSGECYDCAAHFVWIGERT
                     RQLDHAHVEFVRQIGNPIGVKIGPDITANELLRLIDALNPANDPGRLTLITRMGAERI
                     AEVLPGLVRAVQREGRELVWSSDPMHGNTFKSSTGFKTRRFDQVLLELQRFFAVHRAE
                     GSYPGGIHLEMTGEHVTECTGGAWEISEQDLATRYLTSCDPRLNADQVLELAFLIADA
                     LRQVAPASNDNQIHDARGQT"
     gene            2283..3470
                     /locus_tag="VIW27_06275"
     CDS             2283..3470
                     /locus_tag="VIW27_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011337850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739313.1"
                     /translation="MNKLIMAVVLAAASTTAFADGHVVKIGMLQGFTGPTESLVAPMA
                     LGGELAIKEVSDSGQFLGGKQVVSVRGDSTCIDAGAATAAAERLITSDGVAAINGATC
                     SGATTAVLNNVAIPNGVVMISPSATSPALSTIPDNDLFFRTAPSDARQGQVLADVIKS
                     RGFDTVAVTYTNNDYGKGLADSFKEAFTAVGGKVTIVAAHEDGKGDYSAEVGALSAGG
                     GDLLVVAGYLDQGGKGIIRAALDTGAFDKFVLPDGMVGDSLAEAFGTSFEGSFGTVPG
                     TDSPGAKKMGEVSAGKGFNGTDPYVPECYDASALILLAMQSAGSTDSAKFKSHIMSVA
                     NAPGEKIYPGELGKALKILADGGEIDYVGGTAVELVGSGESAGAFREVEIKGGKFTTV
                     KYH"
     gene            3531..4316
                     /locus_tag="VIW27_06280"
     CDS             3531..4316
                     /locus_tag="VIW27_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739314.1"
                     /translation="MISVVDLHMHFGGIRAVDGTSLQIEKGSITGLIGPNGAGKTTLF
                     NVIAGAYLPTSGKVYLDGEDVTGLPAHQMFAKGMLRTFQIAHEFSSLSVLENLMVVPG
                     NQPGERLRDAWFRPRRVAEHEEGVRRRAREVIEFLEISHLENELAGNLSGGQKKLLEL
                     GRTMMVDAKIVFLDEVGAGVNRTLLKTIGTSIQRLNRERGYTFCMIEHDMDFIARLCD
                     PVIVMAEGKLLTQGTVDEVRSNEEVIEAYLGTGRKQKSRAEQG"
     gene            4319..5023
                     /locus_tag="VIW27_06285"
     CDS             4319..5023
                     /locus_tag="VIW27_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013067578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739315.1"
                     /translation="MPFLAATHMTGGYGGADIIRDCSITVEQGEIAVVVGPNGAGKST
                     AMKAVFGMLELRSGSVVLDGRDITRLAPQERVRIGMGFVPQNRNVFVSLTVEENLEMG
                     AYICEHDIGENLEQVYQLFPVLKEKRAQPAGELSGGQRQQVAVGRALMTRPRLLMLDE
                     PTAGVSPIVMDDLFDRILEIASTGIAILMVEQNARHALEIADHGFVLVQGSNRFTDTG
                     KNLLADPEVRRSFLGG"
     gene            5027..>5614
                     /locus_tag="VIW27_06290"
     CDS             5027..>5614
                     /locus_tag="VIW27_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5739316.1"
                     /translation="MDDFLNAVVLLTNFVIVPATAYGAQLALGALGVTLIFGILRFAN
                     FAHGELMAFGTMITILFTWFLQSAGIGIAPLPTALLALPIGIAVTAALALVSDRMVYR
                     FYRSRRSDPIIMAIVSVGVMFVMAGLIRFIIGPDDQSFADGTRFLITAGEFKRATGLG
                     EGLALKQSQVLTLVIAIAVVAALFWFLQKTRAGKAM"
BASE COUNT          984 a         1764 c         1838 g         1028 t
ORIGIN      
        1 cagcgcacgg cagtagcggc gcaccttgtc gacctcgaag cagtactggg tctggaaaaa
       61 ttgcgtgccg ttgtcgatct tcgcctgcag gcgcgccgcg ggccagtgtt cgcccggttc
      121 ggcgggcatc tcggcggcgc cgagaaacag ctgcggcgcc ggatcgatcg cgcggccggt
      181 cgggaattgc ccgagatcgc gcatgtcgcg tatctggcgc atcagttcac cgctgtcgat
      241 ttcgaacacc tccgcggcag cgggctggtc gccggcggcc atcttgtcgc cggtcaggca
      301 caggacgttg cgaatgccga gtgcggcggc gccgaccacg tcgccctgca gcgccagccg
      361 gttacggtcg cgggtggtca tctgcagcac cggttcgatg ccctcgctgg ccatcaccgc
      421 ggcggccgcc agggccgcca tgtgcacgcg cgcgctggcg ccgtcggtga cgttgaccgc
      481 gtcggccagg ccgcgcaggc actcgacgcg ctcgacgacg gcctgcgccg aagccgcatc
      541 gggcggcgag gtttcggcgg ttagcgcgaa atgaccgtcg cgcagcactt gttcgagttt
      601 tcccatcgat gttgtcatgt ctgtcccctg gcatcgtgaa tctggttatc gttgctcgcc
      661 ggcgccacct ggcgcagcgc atcggcgatc aggaaagcga gttccagcac ctggtcggcg
      721 ttcagccgcg ggtcgcagct ggtcaggtag cgggtggcga gatcttgctc ggatatttcc
      781 caggcgccgc cggtgcattc ggtgacgtgt tcgccggtca tctcgaggtg aatgccgccg
      841 gggtagctgc cttccgcccg gtgcacggca aagaagcgtt gcagctcgag cagtacctgg
      901 tcgaagcggc gggtcttgaa gccggtactc gatttgaagg tgttgccgtg catcggatcc
      961 gacgaccaga ccagctcgcg gccctctcgc tgcaccgcgc gcaccagccc cggcaacacc
     1021 tcggcgatac gctcggcgcc catgcgtgtg atcagcgtca ggcggccggg atcgttggcc
     1081 ggattcagcg cgtcgatcag ccgcagcagc tcgttggcgg taatgtccgg gccgattttg
     1141 acaccgatcg ggttgccgat ctgcctgaca aactcgacat gggcatgatc gagctgccgc
     1201 gtgcgctcgc cgatccagac gaaatgcgcc gcgcagtcgt agcattcgcc gctgaacgaa
     1261 tcgatccgtg acagggcttc ctcgtaattc agcagcagcg cctcgtgcga tgtgaacaag
     1321 cgggtttcgc gaatcgatgg cgcggttgcc gaagtgatgc cgcagacttc catgaacccg
     1381 agcgcatcct cgatgcgcga agcgagctcg aggtagcgct ccttgccggg gctggattcg
     1441 acgaagctca tgttccagcg atgcacctgg tgcaggtcgg cgagaccgcc ctgggcgaac
     1501 gcgcgcagca ggttcagcgt cgacgcgctc tgattgtaag cctgcagcat gcgttccggg
     1561 tcggggaccc tggccgcggc ggtcggctcc gcggcatgca cgatgtcgcc gcggaagctg
     1621 ggcagttcga tgccgtcgac cagctcggta tcgaccgagc gcggcttggc gaactgtccg
     1681 gcggtgcgcg cgatcttggt caccgggcgg cgtccggcga aggtcagaac cacggccatt
     1741 tgcagcagca cgcgaaaggt gtcgcgtatc ttgccggcat tgaaaccggc gaacgactcg
     1801 gcgcaatcgc cgccctgcag caggaaaccg cgaccgccgg cgaccgcggc caggttttgc
     1861 cgaagatcgc gaatttcctg cgcgaacacc agcggcggca gcgcgcgcag gcgccgttcc
     1921 acgtccgcga gccggcgcgc atcggggtat acgggttgtt gcgcaatcgg cagtccccgc
     1981 cagctggtgg gtaggtcggc ggcggctttg ccgggaagat tctgcatggt gttaaaaagc
     2041 gcggaataaa cgcgtgaaaa tgcctgaatt cggtacggaa cgccagtact aatcagccgg
     2101 cccggctgca aagatggcag gcgctgcgat tttcgggaaa aattgcgaat ccgcgccaag
     2161 ctggttaaga ttcgccccaa cactggtgtc aatgtcgccg gcgaaagggc taaccgggct
     2221 cgtccggcaa tctttcgaca gataaaaaaa caaatccaca atccttgaag gagagcaaga
     2281 caatgaacaa gcttatcatg gcggtcgtgc tggccgcggc ctcgacgact gccttcgctg
     2341 acggccacgt cgttaaaatc ggcatgctgc agggctttac cggccccacc gaatcgctgg
     2401 tggcgccgat ggcgctcggc ggcgagctgg cgatcaagga agtctccgac agtggccagt
     2461 tcctgggcgg caagcaggtc gtctcggtac gcggcgacag tacctgtatc gacgccggcg
     2521 cggcgaccgc cgcggccgag cgcctgatca cctccgacgg cgtcgccgcg atcaacggcg
     2581 cgacctgttc cggcgccacc accgcggtcc tcaacaacgt cgcgattccc aatggcgtcg
     2641 tcatgatctc gccgtcggcg acttcaccgg cgttatcgac gattcccgac aacgacctgt
     2701 ttttccgcac cgcgccgtcc gatgcccgcc agggccaggt gctcgccgac gtgatcaagt
     2761 cgcgaggttt cgacacggtc gcggtgacct ataccaataa cgattacggc aagggtctcg
     2821 ccgacagctt caaggaagcc tttaccgcgg tcggcggcaa ggtgaccatc gtcgccgcgc
     2881 acgaagatgg caagggcgat tactcggccg aagtcggcgc gttgtcggcc ggtggcggcg
     2941 atctgctggt ggtggccggt tatctcgacc agggcggcaa gggcatcatt cgagccgcgc
     3001 tcgataccgg cgctttcgac aagttcgtgc tgcccgacgg catggtcggc gactcgctgg
     3061 ccgaggcttt cggcacttcg ttcgagggtt ccttcggtac cgtacccggc accgacagcc
     3121 cgggcgccaa gaagatgggc gaggtttccg ccggcaaggg cttcaacggt accgatccct
     3181 acgttcccga gtgctatgac gcgtcggcgc tgatcctgct ggcgatgcag tccgccggct
     3241 ctaccgacag cgccaagttc aagagccata tcatgagcgt cgccaatgcg ccgggcgaaa
     3301 agatttaccc gggagaactc ggcaaggcgc tgaagattct cgccgacggc ggtgaaatcg
     3361 attacgtcgg cggcaccgcg gtcgagctgg tcggctccgg tgaatcggcc ggcgcattcc
     3421 gcgaggtcga gatcaagggt ggcaagttca ccaccgtcaa gtatcactga cagcgtcgca
     3481 ggactaatgc atacggccag aacttcgcgg ttctggccgc tttttttggc atgatttcag
     3541 tcgtcgacct gcatatgcac ttcgggggta tccgcgccgt cgacggtacc tcgctgcaaa
     3601 tcgaaaaagg ttcgatcacc ggattgatcg gccccaacgg cgcgggcaag accacgctgt
     3661 tcaacgtcat cgccggtgcc tacctgccca cttccggcaa ggtctacctc gatggcgaag
     3721 acgtcaccgg cctgccggcg caccagatgt tcgccaaggg catgttgcgt accttccaga
     3781 ttgcacacga attttccagc ctgagcgtgc tcgagaacct gatggtggtc cccggcaatc
     3841 agcccggcga gcgcctgcgt gatgcctggt ttcgcccgcg gcgggtagct gagcacgaag
     3901 agggggtgcg ccggcgggcg cgggaagtca tcgaattcct cgaaatatcc catctcgaaa
     3961 acgagctcgc cggcaatctt tcgggcgggc agaaaaagct gctggaactg ggacgcacga
     4021 tgatggtgga cgccaagatc gtgttcctcg acgaggtcgg cgccggcgtc aatcgcacgc
     4081 tgctgaaaac catcggtacc tcgatccagc gcctcaaccg cgagcgcggt tatacctttt
     4141 gcatgatcga gcacgacatg gatttcatcg cccgcctgtg cgacccggtg atcgtgatgg
     4201 ccgagggcaa gctgctgacc cagggtacgg tcgacgaggt caggtccaac gaggaagtca
     4261 tcgaggctta cctcggcacc gggcgcaagc agaaatcccg cgcggagcag ggctgacgat
     4321 gccgtttctc gctgctaccc acatgaccgg cggctacggc ggcgccgata tcattcgcga
     4381 ctgcagcatc accgtcgagc agggcgagat cgcggtggta gtcggcccca acggcgccgg
     4441 caagtcgacg gcgatgaaag ccgtgttcgg catgctcgaa ctgcgttccg gttcggtggt
     4501 gctcgacggc cgcgacatca ctcggctggc gccgcaggaa cgggtgcgca tcggcatggg
     4561 tttcgtgccg cagaaccgca acgtgttcgt gtcgctgacg gtcgaggaaa atctcgagat
     4621 gggcgcctat atctgcgaac acgatatcgg tgaaaatctc gagcaggtct accagctgtt
     4681 tccggtgctg aaggaaaagc gcgcgcaacc ggccggcgaa ttatcgggcg gccagcgcca
     4741 acaggtcgcg gtgggccgcg cgttgatgac ccgtccgcgg ctgctgatgc tcgacgagcc
     4801 gaccgccggc gtgtcgccga tcgtgatgga cgatctgttc gaccgcattc tcgagatcgc
     4861 cagcaccggc attgcgatcc tgatggtcga gcagaatgcg cgccacgcgc tggagatcgc
     4921 cgatcacggc ttcgtgctgg tgcagggcag taaccgcttt accgataccg gcaaaaatct
     4981 gctcgccgac cccgaggtcc ggcgttcgtt tctcgggggc taactggtgg acgatttcct
     5041 caatgccgtc gtgctgctga ccaatttcgt gatcgtgccg gcgaccgcct acggcgcgca
     5101 gctggcgctc ggcgcgctcg gcgtgacgct gatcttcggc atcctgcgct tcgccaactt
     5161 cgcgcacggc gaattgatgg ctttcggcac catgatcacg atactgttta cctggttcct
     5221 gcagtcggca ggtattggca tcgcgccgtt gccgaccgcg ctgctggcac tgccgatcgg
     5281 catcgcggtc accgccgcgc tggcgctggt cagcgatcga atggtttacc ggttctaccg
     5341 ttcgcggcgc tcggatccga tcatcatggc gatcgtctcg gtcggcgtga tgttcgtgat
     5401 ggccgggctg atccgtttca tcatcggtcc cgacgaccag tcgttcgccg acggcacgcg
     5461 attcctgatc accgccggtg aattcaagcg cgccaccggg ctcggcgagg gcctcgcgct
     5521 caagcaaagc caggtgctga ccctggtgat cgcgatcgcg gtggtcgccg cactgttctg
     5581 gtttctgcaa aaaacccgtg ccggcaaggc gatg
//
LOCUS       DATCZV010000283         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_234843, whole genome shotgun sequence.
ACCESSION   DATCZV010000283 DATCZV010000000
VERSION     DATCZV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234843"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /gene="rlmD"
                     /locus_tag="VIW27_06295"
     CDS             complement(<1..1090)
                     /gene="rlmD"
                     /locus_tag="VIW27_06295"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY5739317.1"
                     /translation="MSRRNRRHQRPREPRELEIESLSHEGRGIARINGKTVFVSAALP
                     GERVLARIVRGRRKFDEATTVEVLQASPARIEPRCAAFAVCGGCSLQHLSAGDQLAHK
                     QQTLLAMMAHAGIEIGALLPPLVGETWGYRQKARLGVKYVDGKGRALVGFRERDTPFI
                     ADMHRCEVLLPQVGHQLEALSALIGGLEARRSIPQIEVAADDTRVQLVFRHLQPLSSA
                     DRERLVEFAKATGFYLQLQPGGPGTVHDLHPQGQSLAYDPLGDGRLRIEFKAIDFVQV
                     NAGINRQMVAQALDLLELSPEHRALDLFCGLGNFTLPMALRCGRVTGVEGDEAMVGRA
                     RDAARANGIDNAEFAVGDLSRADPGLPWM"
     gene            complement(1077..1862)
                     /locus_tag="VIW27_06300"
     CDS             complement(1077..1862)
                     /locus_tag="VIW27_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5739318.1"
                     /translation="MINNLLVFDIETIPDIDGCRRLYDLHGLSDDDVARAVFQLRRQQ
                     AGTDFLRHHLHRIVAISTLLRSGEQFKIWSLGTPESDEQDLLQRFFSGIERYTPRLVS
                     WNGGGFDLPVIHYRSLLYPLNAGHYWETGQNDSSFRYNNYLSRFHERHTDLMDVLAGY
                     QPRAAAPLDEIAGLCGFPGKMGMSGAKVWDAYQQGRIEEIRDYCETDVLNTYLVYLNF
                     ERSRGNLDPVGYRAECQLVRDSLQASGRQHLIDFESAWEHEQA"
     gene            complement(1879..>2359)
                     /locus_tag="VIW27_06305"
     CDS             complement(1879..>2359)
                     /locus_tag="VIW27_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5739319.1"
                     /translation="AKMLDQFANPDNPRAHYENTGPEIWRQSERQITHFVSSMGTTGT
                     IMGCARYFAEVEPNIQIVGVQPTDGTSIPGIRRWPEEYVPRIYRPEAVDRIIDVSRDE
                     AERVTRELAAREGILCGISSGGAVSASLRLAAEVENAVIVTIICDRGDRYLSAGVFH"
BASE COUNT          402 a          766 c          778 g          413 t
ORIGIN      
        1 gcatccacgg caatccggga tcggcgcggc tgagatcccc caccgcgaat tccgcgttat
       61 cgatgccatt ggcgcgcgcc gcgtcgcgcg cgcgcccgac catcgcctcg tcaccctcga
      121 cgccggtcac ccggccgcag cgcagtgcca tcggcagggt gaaattgccg aggccgcaaa
      181 acaggtccag cgcccggtgc tcagggctca attcgagcaa atcgagcgcc tgcgcgacca
      241 tctgccggtt gatgccggcg ttgacctgga cgaaatcgat ggccttgaac tcgattcgca
      301 ggcggccgtc gccgagcggg tcgtaggcca gcgactgacc ctgcgggtgg aggtcatgca
      361 cggtaccggg gccgcccggc tgcaactgca ggtaaaaccc cgttgccttc gcaaactcga
      421 ccagccgctc gcgatcggcg ctcgacagcg gttggaggtg gcgaaatacc agttgtacgc
      481 gggtgtcgtc ggcggcgacc tcgatctgcg gtatgctgcg cctggcctcg agaccgccga
      541 tcagcgcgga cagcgcctcg agctggtggc cgacctgcgg caacaacacc tcgcagcggt
      601 gcatgtcggc gatgaacggt gtatcgcgct cgcggaaacc gaccagcgcc ctgcccttgc
      661 cgtcgacata cttgacgccg agccgggcct tttgccgata accccaggtt tcgccgacca
      721 gcggcggcag cagcgcaccg atctcgatgc cggcatgggc catcatcgcc agcagcgtct
      781 gctgcttgtg ggcgagctga tccccggcgg acaggtgctg caggctgcat ccgccgcaga
      841 ccgcgaaggc cgcgcagcgc ggctcgatgc gggccggcga tgcctgcagc acctcgacgg
      901 tggtggcctc gtcaaatttg cgccggccgc gcactatgcg cgccagcacc cgctcgcccg
      961 gcagggccgc ggatacgaac accgttttac cattgatgcg cgcgatgccg cggccttcgt
     1021 gtgacaggga ttcgatctcg agttcgcgcg gctcgcgagg ccgctggtgg cggcggttac
     1081 gcctgctcat gttcccaggc ggactcgaaa tcgatcaggt gctggcggcc ggaggcttgc
     1141 agcgaatcgc gcaccagctg gcactcggca cggtaaccca ccgggtcgag gttgccgcgg
     1201 ctgcgttcga agttcaggta gacgaggtag gtattgagca cgtcggtttc gcagtaatcg
     1261 cgaatttcct cgatacgtcc ctgctggtag gcgtcccaga ctttcgcacc gctcatgccc
     1321 atcttgcccg gaaagccgca caggccggcg atttcgtcga gcggcgccgc ggctcgcggc
     1381 tggtacccgg cgagcacgtc catcaggtcg gtatgccgct cgtggaagcg gctcaggtaa
     1441 ttgttgtaac gaaagctgct gtcgttctgg ccggtctccc agtaatgccc ggcgttgagc
     1501 gggtacagca ggctgcggta gtggatcacc ggcaggtcga aaccgccgcc gttccacgac
     1561 acgagtcgcg gcgtgtagcg ctcgataccg ctgaaaaagc gttgcagcag gtcctgctcg
     1621 tcggactccg gcgtgcccag cgaccagatc ttgaactgct cgccgctgcg cagcagcgtc
     1681 gagatggcga cgatacgatg caggtggtgg cgcaaaaagt cggtgccggc ctgttgccgg
     1741 cgcaactgga ataccgcgcg cgcgacgtcg tcgtcggaca gaccgtgcaa gtcgtagagc
     1801 cgccggcagc cgtcgatatc gggaatggtt tcgatgtcga atacgagcag attgttgatc
     1861 acagcaacac gccagtcatc agtgaaaaac cccggccgaa aggtagcggt cgccgcggtc
     1921 gcagatgatg gtaacaatga ccgcgttctc gacctcggcc gcgagcctga ggctggcact
     1981 gaccgcgccg ccggaggaaa tgccgcacaa tatgccctcc ctggccgcca gttcgcgcgt
     2041 cactcgctcg gcctcgtcgc gtgacacgtc gatgatacga tcgaccgcct cggggcggta
     2101 gattcggggc acgtattcct cgggccagcg gcgaattccg gggatcgacg taccgtccgt
     2161 cggctgcacg ccgacgatct ggatgttcgg ctcgacctcg gcgaagtagc gcgcacaacc
     2221 catgatcgtg ccggtagtac ccatcgaact gacgaaatgc gtgatttgcc gttcgctctg
     2281 gcgccagatt tccggtccgg tattttcgta gtgcgcgcgc gggttgtcgg gattggcgaa
     2341 ctggtcgagc atcttcgcc
//
LOCUS       DATCZV010000284         5404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_393375, whole genome shotgun sequence.
ACCESSION   DATCZV010000284 DATCZV010000000
VERSION     DATCZV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393375"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..3163
                     /locus_tag="VIW27_06310"
     CDS             77..3163
                     /locus_tag="VIW27_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5739320.1"
                     /translation="MFTDYFIHRPVLSLVISTMIVLLGVQAFFNTQVRQYPELETTVI
                     SITTAYPGASAELIQGFISTPVQQVVSSTEGIDFVRSSSSNSVSRVEVHMKLGFDADA
                     ALTEVSAKVASIRGELPAESEDPVVAKESAQGFALAYYSFYSETLSDVEVTDYLLRTV
                     QPALSTVRGVAEAEILGGKTYSMRVWMDPIRMAALNVSAADIVTAIGAENYQSAAGQT
                     RGRLVQTDVNAVTDSSDPDVFRRFVIRDDGLNAVRLGDVADIELGAESYDSLVMFDGV
                     PSIYIGINGTTDGNPLTTIQLVNEELAKLQKSFPPGLNAAIAYDSTEFIQASINEVIR
                     TLALAAVIVVTVIFLSLGSFRSTLVPLVTIPLSIIGVVFYMQIMGYSINLLTLLAMVM
                     AVGMLVDDAILVVENIYRHIGEGQRPLQAALLGAREIAKPVIATTLVLGVVYAPIGLL
                     GGLTGVLFQEFAFTLVGTIIISTIIALTLSPMMCSRVLKPTISNNAYARFINARFDRL
                     RATYESLLASSLKTRSVTLVFVFFIVAMIVFILFPPPPMKFVQHELAPEEDQGVIFVF
                     SQAPDHSSIDYLNKYTAPYIDIFRQYRDEYESSFMANGFAGPTVSFAGMLLKPWESRS
                     LSSKQMIPQIQRQLDQQPGLQSFAFNPSSLPGSSGNTPVEFLLKTQESYDQLYGTVQQ
                     VLERARQSGKFYFIKSDFNFSKPEIRVSIDRERAESMGISMREIGQTLGAIMAEGYVS
                     RFNLAGRSYKIIPQARREQRENAEIVGQYYVYTDQGEPVALSSLVDIEYKSVPRELLQ
                     FQQQNAAKIDAVPAVPLAEALQVLREISAEVAPAGYTFDYQGQSRQLVQEGSVLAVTF
                     GFAVIVVFLVLAAQYESLRDPLIILTTVPLAMFGALVPMFLGLVSMNIYSQVGLITLI
                     GLVSKHGILIVEFANRIQASDNLPPIEAVTRAASVRLRPVLMTSFSTVLGIMPLVIAS
                     GAGAASRQAIGITIVTGFTIGTLFTLFVLPVIYTFFAGDHRPSIDESEALAPSQLV"
     gene            complement(3131..4072)
                     /gene="gcvA"
                     /locus_tag="VIW27_06315"
     CDS             complement(3131..4072)
                     /gene="gcvA"
                     /locus_tag="VIW27_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013300040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEY5739321.1"
                     /translation="MSRLPPLKSLQAFEAAGRHLSFTEAARELNVTPGAISQQMRLLE
                     EFLEVRLFKRLNRAIVLTDAGQMFLPSISQGFAHFDEAVALLRNQRADGPLTITAAAS
                     FISNWLIPRLGRFKARHPEIDVRIDTSNRLIDFMHEDIDLGIRFGNGVYPQLDTVFLF
                     SYDLIPVCAPHLMQTGNGLAKIADLNHHTLLHSDYRNLDAAFPDWDMWLKVVGADDVD
                     SGHGIYFNQADQLLQAALDGQGVALLANVMVERGLAEGRLVQPFSTRLPVKMSYHLVT
                     SPAKARLAKVSAFREWIVEESAYLREASDELRRSERL"
     gene            4186..4629
                     /locus_tag="VIW27_06320"
     CDS             4186..4629
                     /locus_tag="VIW27_06320"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEY5739322.1"
                     /translation="MKYFQFAIASIVDANTGYGLAQGGGADYRAQELRSRAIRSQSVH
                     GLLGAIGERVRDTVAAYREKLHQRREIDELAQLSDYYLDDIGLTRGDITAVRLGQSSL
                     EQLNADRRTRLAAAPLDYLETQRVDTRAANADAVNEAAYAEAKCA"
     gene            complement(4727..>5404)
                     /gene="nudC"
                     /locus_tag="VIW27_06325"
     CDS             complement(4727..>5404)
                     /gene="nudC"
                     /locus_tag="VIW27_06325"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY5739323.1"
                     /translation="HRDEHLEALLDDNEFVDLREIGWLLDRQQAAQLAYARGLAFWNR
                     HHRFCGSCGSPTESRNGGHLRCCSNRDCGRVHFPRTDPAVIMLVEDRSDPARPRCLLA
                     RNARFPSRMMSTLAGFVDPLESLEETVAREVYEECGIRVGEVIYQASQPWPFPSSIML
                     GFRAQALNREIVIDNREIEEAYWFDAHQLLEFGEWGDDGDGFCLPRRDSIARYLIGSW
                     MEEVLER"
BASE COUNT         1050 a         1698 c         1604 g         1052 t
ORIGIN      
        1 tgaagctcta cccgggcctc agggtagtgg tcgtcgacga cgtgcccgag tactcgcgca
       61 acgccgagta accccgatgt ttaccgatta tttcattcat cgcccggtgc tgtcgctggt
      121 gatcagtacg atgatcgtgc tgctcggtgt tcaggcgttc ttcaataccc aggtgcgcca
      181 gtacccggaa ctggaaacta cggtgatcag catcaccacg gcatacccgg gtgccagcgc
      241 ggagctgatc cagggattca tcagcacccc ggtacaacag gtggtctcca gcaccgaggg
      301 tatcgatttc gtgcgctcca gcagcagcaa cagcgtatcg cgggtcgagg tgcacatgaa
      361 actcggcttc gacgccgacg ccgcgctgac cgaggtcagt gccaaggtgg cgtcgatccg
      421 cggggagctg cccgccgagt cggaggaccc ggtggtcgcc aaggagtcgg cgcagggctt
      481 cgcgctcgcc tattacagtt tctacagtga aaccctgtcc gacgtcgagg tcaccgatta
      541 cctgctgcgc accgtgcaac ccgcgctgtc gaccgtgcgc ggcgtcgccg aggcggaaat
      601 tctcggcggc aagacttatt cgatgcgtgt ctggatggac ccgatacgca tggccgcact
      661 gaacgtctcg gcggccgata tcgtcactgc gatcggtgcc gaaaactacc agtccgccgc
      721 cggtcagact cgtggccggc tggtgcagac cgatgtcaat gcggtcaccg attccagtga
      781 tcccgacgtg tttcggcgct tcgtgattcg cgacgacggc ctcaacgcgg tgcgtctcgg
      841 cgacgtcgcc gatatcgagc tcggcgccga gagttacgat tcgctggtta tgttcgacgg
      901 cgtaccgtcg atctatatcg gcatcaacgg caccaccgac ggcaatccgc taaccaccat
      961 tcaactggtc aacgaggaac tggcgaaact gcaaaaatca ttccccccgg gcctcaatgc
     1021 cgcgatcgcc tatgactcga ccgagttcat ccaggcgtcg atcaacgaag tcatccggac
     1081 cctggcgctg gcggcggtga tcgtggtcac ggtgattttc ctgtcgctgg gttcgtttcg
     1141 ctcgaccctg gtgccgctgg tcacgattcc actgtcgatc atcggcgtcg ttttctacat
     1201 gcagatcatg ggctactcga tcaacctgct gacgctgctg gcgatggtga tggcggtcgg
     1261 catgctggtc gacgatgcga tcctggtggt cgaaaacatc taccgtcata tcggcgaggg
     1321 ccagcggccg ttgcaggccg cgttactcgg cgcgcgcgag atcgccaaac cggtgatcgc
     1381 gacgaccctg gtgctcggcg tggtctatgc gccgatcggc ctgctcggcg gacttaccgg
     1441 ggtgctgttt caggaattcg cgttcacgct ggtcggcacg attatcatct cgacgattat
     1501 cgcgctgacc ctgtcgccga tgatgtgttc gcgcgtgctc aaaccgacga tcagcaacaa
     1561 tgcctacgcg cgcttcatca acgcgcgctt cgaccggctg cgcgccactt acgaaagcct
     1621 gctggcgtcg tcgctgaaaa cccgctcggt gaccctggtg ttcgtgtttt tcatcgtcgc
     1681 catgatcgtc ttcatcctgt ttccgccgcc gccgatgaaa ttcgtccagc acgagctggc
     1741 accggaggaa gatcagggcg tcatcttcgt gttttcgcag gcacccgatc acagcagcat
     1801 cgattacctg aacaagtaca cggccccgta catcgatata tttcgccagt accgcgacga
     1861 atacgaatcg tcgttcatgg ccaatggttt cgccgggccg acagtttcct tcgccggcat
     1921 gctgctcaaa ccctgggagt cgcgcagcct gtcctcgaag cagatgattc cgcaaatcca
     1981 gcggcagctg gaccagcagc ccggcctgca gagctttgcc ttcaatccgt catcgctgcc
     2041 cggctccagc ggcaacaccc cggtcgaatt cctgctcaag acccaggaga gctacgacca
     2101 gctctatggg accgttcagc aggtgctgga acgcgcgcgc cagagcggca agttttattt
     2161 catcaagagt gatttcaatt tttccaagcc cgaaatccgc gtcagcatcg accgcgaacg
     2221 cgccgagtcg atgggtattt cgatgcgcga gatcggccag acgctgggcg cgatcatggc
     2281 cgagggttac gtcagccgct tcaacctcgc cgggcgcagt tacaagatca ttccgcaggc
     2341 gcgccgcgag cagcgcgaga atgccgagat cgtcggccag tactacgttt acaccgacca
     2401 gggagagccg gtagcactgt cgtcactggt cgacatcgag tacaaatccg tgccccgcga
     2461 gctactgcag ttccagcagc aaaacgcggc caaaatcgac gcggtgcccg cggtaccgct
     2521 cgccgaggcg ctgcaggtgc tacgcgagat cagcgcggaa gtcgcgccgg cgggttatac
     2581 cttcgattac cagggacagt cgcgccagct ggtgcaggaa ggctcggtac tggcggtgac
     2641 cttcggtttc gccgtcatcg tcgtcttcct ggtgctggcg gcgcaatacg agagcctgcg
     2701 cgacccgctg atcatcctca ccacggtgcc actggcgatg ttcggcgccc tggtgccgat
     2761 gttcctcggc ctggtgtcga tgaatattta cagccaggtc ggcctgatca cgctgatcgg
     2821 gctggttagc aagcacggca tcctgatcgt cgaattcgcc aaccgcatcc aggcctccga
     2881 taatctgccg ccgatcgagg cggttaccag ggcggcctcg gtgcgcctgc gcccggtgct
     2941 gatgacctcg ttctcgaccg tgctcggtat catgccgctg gtcatcgcca gcggggccgg
     3001 cgccgccagc cgtcaggcaa tcggtattac gatcgtcacc ggctttacca tcggcacgct
     3061 gttcacgctg ttcgtgctgc ccgtcatcta cacttttttc gccggcgatc accgcccgtc
     3121 gatagacgaa tcagaggcgc tcgctccgtc gcaactcgtc tgaagcctcg cgcaggtagg
     3181 ccgactcttc gacaatccat tcgcgaaatg cgctgacctt ggcgaggcgt gccttggccg
     3241 gcgatgtcac gaggtggtaa ctcatcttca ccggcaggcg ggtgctgaat ggctgcacca
     3301 gcctgccctc ggccagcccg cgctcgacca tgacattggc gagcaacgcc acgccctggc
     3361 catcgagcgc ggcctgcaac agctggtcgg cctggttgaa ataaatcccg tgaccgctgt
     3421 cgacatcgtc cgcgccgacc acttttaacc acatatccca gtcgggaaag gcagcgtcga
     3481 gattacggta atcgctgtgc aatagcgtgt gatgattgag atccgcgatc tttgcgagcc
     3541 cgttgccggt ttgcatcaaa tgtggcgcgc ataccgggat caggtcgtag gaaaacagga
     3601 acacggtatc gagctgcgga tagacgccgt taccgaagcg aatcccaagg tctatatcct
     3661 cgtgcataaa atcgatcagc cggttagagg tgtcgattcg cacgtcgatc tccgggtgtc
     3721 gtgccttgaa acggccgagg cgcggaatca gccagttgga aatgaacgag gccgccgcag
     3781 taatcgtcaa tggcccgtcg gcgcgctggt tgcgcagcaa cgcaacggcc tcatcgaagt
     3841 gcgcgaatcc ctggctgatc gacggcagga acatttgccc ggcgtcggtg agcacgatcg
     3901 cgcgattcag gcgcttgaac aacctgacct cgagaaattc ttcgagcagg cgcatctgct
     3961 ggctgatggc gccgggagtg acgttcaact cgcgcgccgc ctcggtgaag ctcaggtgac
     4021 gcccggcagc ctcgaaggct tgcaggctct tgagcggtgg taatcgactc atattagtta
     4081 atctaaactg actgacaaaa tatatcgttt gtgagctaga tattcaatgc atatattagc
     4141 tctgaaacag attcaaagcc tatattaatt atgagaatta ctaatatgaa atactttcaa
     4201 ttcgcgatcg catcgatagt cgatgccaat accggctacg gcctggcgca gggcggcggc
     4261 gccgattacc gcgcacagga actgcgctcg cgcgcgattc ggtcacaatc cgtgcatggc
     4321 ttgctcggcg ccatcggcga gcgggtgcgc gacacagtcg ccgcataccg tgaaaagctt
     4381 caccagcggc gggaaataga cgaactagcg cagctgagcg attactatct cgacgatatc
     4441 ggcctgaccc ggggcgacat cactgccgtg aggctcggcc agagctcgct cgaacagttg
     4501 aacgcggacc gtcgtactcg cctggcagca gccccgctcg actacctcga aacccaacgg
     4561 gtcgacaccc gcgccgccaa cgccgacgcg gtcaacgagg cagcttacgc ggaggcaaaa
     4621 tgcgcctgac gccgatcgac gagggccggg gcccgcgctg ggtcccggca ttcgtatcac
     4681 cagccatctg ccaccgcagc gaacgcatcg tggcgacggc cagcggctag cgctcgagca
     4741 cttcttccat ccagctgccg atcaggtagc gcgcgatcga atcgcgccgc ggcaggcaaa
     4801 agccgtcgcc atcgtccccc cactcaccga actccagtaa ctggtgcgca tcgaaccagt
     4861 aggcttcctc gatttcccgg ttgtcgatga cgatctcccg gttcaacgcc tgcgccctga
     4921 aaccgagcat gatcgacgat ggaaacggcc acggctgcga agcctggtag atgacttcgc
     4981 cgactcgaat gccacactcc tcgtagactt cgcgggccac ggtttcctcg aggctctcca
     5041 gcggatcgac gaaacccgcc agcgtcgaca tcatgcggct cgggaagcgg gcattacgcg
     5101 ccagcaggca gcgcggccgc gccggatccg agcgatcctc gaccagcatg atgactgccg
     5161 ggtcggtgcg cggaaaatgc acgcgcccgc agtcgcggtt gctgcagcat cgcagatgtc
     5221 cgccgtttcg actttcggtg ggactgccgc aactgccgca gaaacggtga tggcgattcc
     5281 agaaggccag gccgcgggca taagccagct gcgcggcctg ctggcggtcg agcagccagc
     5341 cgatctcacg caaatcgacg aattcgttgt cgtcgagcag agcttcgaga tgctcgtcgc
     5401 gatg
//
LOCUS       DATCZV010000285         3874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_658421, whole genome shotgun sequence.
ACCESSION   DATCZV010000285 DATCZV010000000
VERSION     DATCZV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658421"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VIW27_06330"
     CDS             complement(<1..1709)
                     /locus_tag="VIW27_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739324.1"
                     /translation="MKILRLVTGLGLMAFAGSLWAACPAATVADMKGVPAGKYPQQYD
                     LAEFEKLAGCKMTFRENPDIAKLNGRIRGNPDKLPPVAERIPEDALVMAPYQSIGKYG
                     GTFDVMSNATEAGTSDFLAIRHVNLVRYSDDLQTIVPNVAKGWEWNDDFTQLTFFLRK
                     GHKWSDGEPFTAEDVEYWYEGLSLNPKVFEKPKNYVLVADKPMTVDVLDPQTVRFNLP
                     APKPGLLVHFANSYAQGFQPKHFLGQWDPEINPDADKLAQSMGFKDGYEVISNYYGNS
                     DWTDTPTPMLRSPDKVKNMPKDTMPTLESHILISESTEGRHLVANPYFFQVDTAGNQL
                     PYISEQDEIFVNEDEVRLLKLVNAEVDFKAQSLQLSTAPLLLENQEKGDYTVHLRPEV
                     TIASFGINMTHENLEKRKVFADLRFRQAMSVAMNREEMNEVAFFGMGEPKQYTGFIPL
                     PPFADQKYNQYYAQYDPALANKLLDEIGLKDRDGDGFRELPNGDRLVINLQFSVQGVP
                     AAMVELIGQHWAAVGIQTTVKEVTPDEYRSAQSANKLDVTMWRNGQPLAIVLGTNQIW
                     VPP"
     gene            complement(1933..3135)
                     /gene="glcF"
                     /locus_tag="VIW27_06335"
     CDS             complement(1933..3135)
                     /gene="glcF"
                     /locus_tag="VIW27_06335"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003256161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY5739325.1"
                     /translation="MQTNLAKRFREHAEAAHIDAILRKCVHCGFCNATCPTYQLLGDE
                     LDGPRGRIYQIKQFLEGEPANAEMLKHLDRCLTCRSCETTCPSGVKYSHLLEFGKTAI
                     ERELPRAGWDRWRRGAIVRFIHSGWPFGFALRLGQFFKPLLPGPLGRAVPRRQKLVAR
                     SGGSHQRKVLMLAGCVQPVLTPNTNRLAINLLDRCGIEVIESRDGLCCGAAAMHTSNP
                     ELALEQARRLIDAWWPHIEAGAEAIVVSATGCGVNVADFGRLLAADSEYAGKAARVSE
                     LFRDLVDIVEAEIDSLEPGAGAGRRVAVHTPCTMQHGLGLGGRVERLLEQRGYQICTV
                     AEGHLCCGSAGTYSMLQPALAKRLRRNKLAALTGDSPQIIATANIGCQVHLTDPSEPP
                     VVHWIELV"
     gene            complement(3138..>3874)
                     /locus_tag="VIW27_06340"
     CDS             complement(3138..>3874)
                     /locus_tag="VIW27_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5739326.1"
                     /translation="RRAFAGGIRDFVLGVRMLDGRGQLLNFGGRVIKNVAGFDLARAL
                     VGSLGTLGVILEVSIRVLPVPETELTLAFEHATPGEHIRWVNGLGGRPWPISAAMWQA
                     GRSWLRLSGSEQGVRHAARELGGDQVDPPWQELREQRLEFFDPQQPLIRVTLPPTHAD
                     LPVDLPQLIEWGGALRWFSGNGNIADMRAGLEAAGASVCAYRGHDARVGLFHPLPEAM
                     LRLHRSLKSSFDPAGIFNPGKMYPGL"
BASE COUNT          667 a         1232 c         1246 g          729 t
ORIGIN      
        1 tacggcggta cccagatctg gttggtgccg agcacgatcg ccagcggctg gccgttgcgc
       61 cacatggtaa cgtcgagctt gttggccgat tgcgcgctgc ggtattcgtc cggcgtgacc
      121 tctttgaccg tggtctggat gccgaccgcg gcccagtgct ggccgatcag ctcgaccatc
      181 gccgccggta cgccctgcac cgagaactgc aggttgatga ccagtctgtc gccgttcggc
      241 agttcgcgga aaccgtcgcc gtcccgatcc ttgaggccga tctcgtcaag cagcttgttg
      301 gccagcgccg gatcgtactg ggcgtaatac tggttatact tttgatcggc gaacgggggt
      361 agcggaatga aaccggtata ctgcttgggt tcacccatac cgaagaaggc gacctcgttc
      421 atttcctcgc gattcatcgc caccgacatc gcctggcgga agcgcaggtc ggcgaaaacc
      481 ttgcgttttt ccaggttctc gtgggtcatg ttgatgccga agctcgcgat cgtcacctcg
      541 ggtcgcaggt gcaccgtgta atcgcccttc tcctggtttt cgagcagcag cggcgcggtc
      601 gacagctgca gcgactgcgc cttgaagtcg acctcggcgt tgaccagctt gagcagacgc
      661 acctcgtctt cattgacgaa gatttcatcc tgttcgctga tgtagggcaa ctggttgccg
      721 gccgtgtcga cctggaagaa atacgggttg gcgaccaggt ggcggccctc ggtggactcc
      781 gaaatcagga tatgcgactc cagcgtcggc atcgtatcct tgggcatatt cttgaccttg
      841 tccgggctgc gcagcatcgg cgtcggcgta tcggtccagt ccgaattacc gtagtaattg
      901 gaaatcacct cgtaaccatc cttgaatccc atcgactgcg ccagcttgtc ggcatcggga
      961 ttgatctccg gatcccactg gccgaggaag tgcttgggct gaaaaccctg cgcgtaggaa
     1021 ttcgcgaagt gcaccagcag gcctggcttc ggcgccggca ggttgaagcg caccgtttgc
     1081 ggatcgagga catcgaccgt catcggcttg tcggcgacca gcacatagtt cttcggcttt
     1141 tcgaacacct tcgggttcag cgacagtccc tcgtaccagt attcgacgtc ttccgcggta
     1201 aacggctcgc cgtcggacca cttgtggccc ttgcgcagga agaaggtcaa ctgggtaaaa
     1261 tcatcgttcc actcccagcc cttggcgacg ttcggcacga tggtctgcag atcgtccgag
     1321 taacgcacca ggttgacgtg gcgaatggcg aggaagtcgg aggtgccggc ctcggtggcg
     1381 ttcgacatta cgtcgaaggt gccgccgtac ttaccgatgc tttggtaggg cgccattacc
     1441 agcgcatctt cgggaatgcg ctcggccacc ggcggcagct tgtcggggtt gccgcgaatc
     1501 cggccgttga gcttggcaat atccgggttc tcgcggaacg tcatcttgca tccggcgagt
     1561 ttttcgaact ccgccaggtc atattgttgg gggtatttac cggctggcac gcctttcatg
     1621 tcggccactg ttgccgccgg gcaagccgcc cacaggctgc cggcaaaggc catcaggccc
     1681 agtcctgtaa caagtcgtaa tattttcatc ggaagtctcc tcgcgagccc ttctggttat
     1741 ttagagatat tattggttgt agccctgcgc atcgcgcgcc aggctgccta aaactaacct
     1801 acctaaaggc aataatcaaa tactccttta ggcctggcaa atggttgcag gggaatatga
     1861 cgaaacggtg acatcgggcg tcgattccgc gcgccgcacg acccgtgcca cccggccggg
     1921 tcatggcaat cttcaaacca gctcgatcca gtgcaccacc ggcggctcgc tcgggtcggt
     1981 tagatgcacc tggcagccga tattggcggt ggcaatgatt tgcggactgt cgccggtcag
     2041 cgccgcgagc ttgttacggc gcaggcgttt tgccagcgcc ggttgcagca tcgagtaggt
     2101 accggccgaa ccgcagcaaa gatggccctc ggcgacagtg cagatctggt aaccgcgctg
     2161 ctcgagcagg cgctcgacgc ggccgccgag gccgagcccg tgctgcatcg tgcagggcgt
     2221 atgcacggcg acgcggcggc cggcgccggc gccgggctcg agcgaatcga tttcggcctc
     2281 gacgatatcg accaggtcgc gaaacagttc gctgacccgc gccgccttgc cggcgtattc
     2341 cgaatccgcc gccagcagtc ggccgaagtc ggcgacgttg acgccgcaac cggtggcgct
     2401 aaccacgatc gcctcggcgc cggcctcgat atgcggccac caggcatcga tcaggcgccg
     2461 ggcctgttcc agggccagct cgggattcga ggtgtgcatg gccgccgctc cgcagcacag
     2521 gccgtcgcgc gattcgatga cctcgatacc gcagcggtcg agcaggttga ttgccaggcg
     2581 gttggtattc ggcgtcagta ccggctgcac gcagccggcc agcatcagca ccttgcgctg
     2641 gtggcttccg ccgctgcgcg ccaccagctt ctgccgccgc ggcaccgcgc gccccagggg
     2701 cccggggagc agcggcttga agaattgccc gagccgcagc gcgaacccga acggccagcc
     2761 cgagtggatg aagcgcacga tcgctccacg gcgccagcgg tcccagcccg cacgcggcaa
     2821 ttcacgctcg atcgcggtct tgccgaattc cagcaggtgg ctgtacttga cgcccgacgg
     2881 gcaggtggtt tcgcaggagc ggcaggtcag gcagcggtcg aggtgcttga gcatctcggc
     2941 attggcgggc tcgccctcga gaaattgctt aatctggtag atgcggccgc gcggtccgtc
     3001 gagctcgtcg ccgagcagct ggtaagtcgg gcaggtcgcg ttgcagaaac cgcagtgcac
     3061 gcacttgcgc aggatcgcgt cgatatgcgc ggcctcggcg tgctccctga agcgcttggc
     3121 gagattggtc tgcatggcta gaggcccggg tacatttttc cggggttgaa gatgccggcc
     3181 ggatcgaagc tcgacttcag gctgcggtgc aggcgcagca tggcttcggg cagcggatga
     3241 aacaggccga ctcgcgcgtc gtggccgcga taggcgcaga cgctggcacc ggccgcttcc
     3301 aggccggcgc gcatatcggc gatattgccg ttgccgctga accagcgcag cgctccgccc
     3361 cactcgatga gctgcggcag atccaccggc aggtccgcat gcgtcggcgg caaggtaacc
     3421 cggatcaacg gttgctgggg atcgaaaaac tcgagccgtt gctcgcgcag ctcctgccac
     3481 ggcggatcca cctggtcgcc gccgagttcg cgcgccgcgt ggcgcacgcc ctgctcgctg
     3541 cccgacagcc gcagccagct gcgaccggcc tgccacatcg ccgccgagat cggccacggg
     3601 cgcccgccga ggccgttgac ccagcgaatg tgctcgccgg gcgtggcgtg ttcgaaggcc
     3661 agcgtcagct cggtctcggg taccggcagc acgcgtatcg atacctcgag gatcacgccc
     3721 agcgtgccca gcgaaccgac cagcgcgcgc gccaggtcga agccggcgac gttcttgatc
     3781 acgcggccgc cgaaattcag cagctggccg cggccgtcga gcatgcgcac gccgagcacg
     3841 aaatctcgaa tgccgccggc aaacgcgcgg cgcg
//
LOCUS       DATCZV010000286         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_23428, whole genome shotgun sequence.
ACCESSION   DATCZV010000286 DATCZV010000000
VERSION     DATCZV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23428"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..676)
                     /locus_tag="VIW27_06345"
     CDS             complement(5..676)
                     /locus_tag="VIW27_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY5739327.1"
                     /translation="MRTQALNSIAHGQSSAIAVNKVLRNTYALLSMTLLFSALNAGIS
                     MSLNLPHPGLLLTLIGYFGLLFATTRLRNSGWGLVSVFALTGFMGYTLGPILNAYLAL
                     ANGGQLVMTAMGATGAIFLGLSGYALVSRRDFSFMASFLVVGILLGFLAGLGAVVFQI
                     PALALAVSAMFVLLMSGLILYETSNIVHGGETNYIMATVTLFVSIFNLFTSLLHLLGF
                     LGGQE"
     gene            complement(702..1085)
                     /locus_tag="VIW27_06350"
     CDS             complement(702..1085)
                     /locus_tag="VIW27_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739328.1"
                     /translation="MNIDIQANAIPAHKDVHHFVACRADLALGGMRDQLALVSVFIDG
                     SGAGDEVRCLVMIRPYAQPDIIVEGCHANVYVAVHQTLDEAGWTLARKLASQQNAFID
                     RQFELLGDQAARSGMRGLPLSGRAA"
     gene            1194..2084
                     /gene="nhaR"
                     /locus_tag="VIW27_06355"
     CDS             1194..2084
                     /gene="nhaR"
                     /locus_tag="VIW27_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEY5739329.1"
                     /translation="MINYKHLHYFWVVAKQGGIARASEILHLTPQTISGQINLLEDNL
                     GQALFTRSGRNLELTEAGRLALGYADEIFSLGSQLEENLRNQTTDRPLLFKVGIADVV
                     PKTIAYRLLSPALELPDSIRIQCRESSLETLLAELALHRVDMVLADGPIPPGIEVRGF
                     NHPLGECGISFFGKAELARDLVGGFPRSLGGAPLLIPSDINRVHDRLLQWLEGMRIFP
                     QIIGEFDDSALMKVFGQAGDGIFVAPTAIADEVMEHYGVEQIGQTEAVREQFFAISVE
                     RRISHPAVSAITEAAKRWLQ"
BASE COUNT          474 a          673 c          651 g          391 t
ORIGIN      
        1 acattcattc ctggccgccc agaaaaccga gcaaatgcag caggctggtg aacaaattga
       61 aaatcgacac gaacagcgtc acggtcgcca tgatgtaatt ggtctcaccg ccgtgcacga
      121 tattactggt ctcgtagagt atgagacccg acatcagcag cacgaacatc gcagataccg
      181 ccagcgccag ggccggaatc tggaacacca ccgcgcccag cccggcgaga aacccgagca
      241 ggatgccgac caccaggaat gaggccatga aactgaaatc gcggcggctg accaacgcgt
      301 agcccgacag gccgaggaag atggcgccgg tggctcccat cgcggtcatc accagctggc
      361 cgccgttggc gagcgccagg taggcattca aaatcggtcc cagcgtgtaa cccatgaacc
      421 cggtcagcgc aaataccgac accaggcccc agccgctgtt gcgcaggcgg gtggtcgcga
      481 acagcaggcc gaagtatccg atcagcgtca gcagcagacc cggatgcggc aagttgagcg
      541 acatcgatat acccgcgttc aacgcgctga acagcagcgt catcgacagc agcgcatagg
      601 tattgcgcag caccttgttt acggcgatcg cactcgactg accgtgcgcg atcgaattca
      661 aagcttgtgt tctcatcttc gtctccctgt cagaaggtta atcaggcggc tcgacccgac
      721 aacggcagac cgcgcattcc ggaacgggcg gcctggtcac cgagcagctc gaattgccga
      781 tcaatgaacg cgttctgctg gctcgcaagt ttgcgcgcca gggtccatcc cgcctcgtcc
      841 agggtctggt gcactgccac gtaaacgttc gcatggcagc cctcgacgat gatgtcgggc
      901 tgcgcgtagg gacggatcat caccaggcag cgaacctcgt cgcccgcgcc gctgccgtcg
      961 atgaacacgc tgaccagagc gagctggtcg cgcattccgc ccagcgcgag atccgctcgg
     1021 caagcgacga agtgatgcac gtctttgtgg gctggaattg cgttcgcctg tatatcgatg
     1081 ttcatggttt tacccgaaaa caagttgttg acgcagccaa tattagggcg aatcctatga
     1141 aataatattc gaattaaata caatatttat tcggtttttt cgagctatta aaaatgatca
     1201 attacaagca cctgcattac ttctgggtgg tggccaagca gggcggcatc gcccgcgcca
     1261 gcgaaatcct gcatctgacg ccgcaaacca tcagcggcca gatcaacctg ctcgaggaca
     1321 acctgggaca ggcgctgttc actcgcagcg gcagaaacct cgagttgacc gaagccggcc
     1381 ggctggcgct gggctacgcc gacgagatat tcagcctcgg ttcgcaactc gaggaaaacc
     1441 ttcgcaacca gacaaccgac cggccgctgc tgttcaaagt cgggatcgcc gacgtggtac
     1501 ccaagacgat tgcctatcgc ttgctgtccc cggcactcga gctgccggat tcgatacgca
     1561 tccagtgccg cgagagcagc ctggaaacgc tgctcgcaga gctcgcgctg caccgcgtgg
     1621 acatggttct cgccgacggg ccgataccgc ccggcatcga ggtgcgcggt ttcaatcatc
     1681 ccctcggtga gtgcggcatc agtttcttcg gcaaggcaga gttggcgcgc gacctggtcg
     1741 gcggcttccc ccgcagcctc ggcggcgcgc cgctattgat tccgagcgac atcaaccgag
     1801 tgcacgatcg gctgctgcaa tggctggaag gcatgcgtat ttttccgcaa atcatcggcg
     1861 aattcgacga cagcgcattg atgaaagtat tcggccaggc cggcgacggc attttcgtcg
     1921 cgccgaccgc gattgccgac gaggtcatgg aacactacgg ggtcgaacag atcggccaga
     1981 cggaggccgt gcgcgagcag ttcttcgcga tctcggtgga aagaagaatt tcgcatcccg
     2041 cggtgtcggc gattaccgag gcggcgaagc gctggctgca atgacgcccc gggcgattcg
     2101 tggcgggcgc ttcaccgtcg cctggcgcag tcccgccagc aaatcggccc gcggggtccc
     2161 gcggcctgga gcgcggccgg tgtaaagat
//
LOCUS       DATCZV010000287         5688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_553591, whole genome shotgun sequence.
ACCESSION   DATCZV010000287 DATCZV010000000
VERSION     DATCZV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553591"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..995)
                     /locus_tag="VIW27_06360"
     CDS             complement(306..995)
                     /locus_tag="VIW27_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5739330.1"
                     /translation="MSQAAIGKRVGNHAFVDATSGRTVSLDSLRGKPLVISMIYTSCY
                     HICPMTTQHLAKVVDKAHATLGEDSFSVALIGFDTAVDDPAAMRYFAARQGVADKGWH
                     ILSGDADSVAALSQDLGFRFYPTSNGFDHLVQASVVDADGTVYRQVYGQVFETPLLVD
                     PLIELVLGRPAPQQTLLDGIVARVKLFCTTYDPVRDGYYYDYSLFLGIFIGAIIILAT
                     ATFIYRELRRH"
     gene            complement(1383..2294)
                     /gene="cyoE"
                     /locus_tag="VIW27_06365"
     CDS             complement(1383..2294)
                     /gene="cyoE"
                     /locus_tag="VIW27_06365"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5739331.1"
                     /translation="MAAELIFGRPGGRGATREVARQLVDLLKLRIGVIMALTALVAML
                     ATPGPRPSFVDALVLFLAVLIASGAAGAFNHYYEVDLDRRMPRTCRRPFVTGYFHRGP
                     LWLIAIAGLLVAGVGSAALLLNAAAALHIFLGAFFYAVVYTVWLKRRSWTNIVVGGAA
                     GSFAVLAGAAAVDPLPGPIPLALALALFLWTPSHFWSLAIAKFADYEKAGVPMLPVIV
                     GKRRAAEIVFANTLALVAVSVMPFFFGLGWIYLAGAVAGGGLFLYRNIRMIRDPSPRV
                     AMRSFLASLLQLVLLLAGAVLDVLLLG"
     gene            complement(2279..4012)
                     /locus_tag="VIW27_06370"
     CDS             complement(2279..4012)
                     /locus_tag="VIW27_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5739332.1"
                     /translation="MAQFRVCPDSGFYFDRSAESLMKANAVAGAVALLVAGILGLGVV
                     LTRWQEVHLLPADLFYQALTGHGLNALIFWIIFFEMAILYFASSTLLRTRLAAPRWAW
                     LGFALMIIGWITNNIAVLQGNSSVMMTSYAPMQAAPHFYLGLILFAVGALIGCFVFLG
                     TLVIARDDRTYEGSIPLVTFGALTACIIAIFTVASGAIILVPTFLWSIGYINHIDAEM
                     YRVIWWGMGHSSQQINVAAHVSIWYLIVGVLFGARPMSEKVSRMAFLLYILFLQLASA
                     HHVLVDPGLSAEYKIFNTSYAMYLAVLASMIHGLTVPGSMEVAQRAKGFTKGMFEWLR
                     KAPWGNPVFSGFFFSLIIFGFLGGITGVVMGTEQINLIIHNTIYVPAHFHATVAVGTT
                     LAFMALTYLLIPVLFRRELFLPWVARWQPYVFSAGMTLMILFMLGAGTLGVARRHWDM
                     DFTGAALAFEYPGTAYTMMAVAGVGGVLAVLGGAMFLLVTVGSLLFGKKLDESAGLLQ
                     SFGVPLRPASYNVEQPERLQMAPKLAVEQHGSAGFEAPGTFALAMALLVCFVVYYFIN
                     WDYLASVWPLS"
     gene            complement(4014..4553)
                     /locus_tag="VIW27_06375"
     CDS             complement(4014..4553)
                     /locus_tag="VIW27_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEY5739333.1"
                     /translation="MSSFLPPAERNWWSSPVGKQEIVWIGIALVWCLIMFLMMPYWHV
                     YGKQNLSSEAYQTTPAKFQSRVDAMVEAHQVGVEAGIPVVRPPAGSDVYMLGRLWQWY
                     PILELQKGQSYRLHLSSMDWQHGFSLQPVNINLQIVPGYEMVLTVAPDQAGEFTVVCN
                     EYCGIGHHLMLGKIYVRGN"
     gene            complement(4572..4733)
                     /locus_tag="VIW27_06380"
     CDS             complement(4572..4733)
                     /locus_tag="VIW27_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739334.1"
                     /translation="MSEQDTIPKPERVPFFQRVLDNPFLLLFLGIVMPTVFYILWGIM
                     EIATIPVAP"
     gene            4994..5470
                     /locus_tag="VIW27_06385"
     CDS             4994..5470
                     /locus_tag="VIW27_06385"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5739335.1"
                     /translation="MTSIEWRNEYRTGIDGVDYEHEILIGQINTLLQMIGKRADRALI
                     LDGLGEIYGSIAAHFALEEQTMQKLGYDEYEPHRADHERLLDDIRDLADEFEGSAEID
                     EQQFGERLASWFGHHFQTHDARLHRLAGANVHQPESGSAMRRMIRDAKQKLLGKHG"
BASE COUNT         1199 a         1745 c         1873 g          871 t
ORIGIN      
        1 ccactgtacc caaacgctgc cacggagggt ttgacattag tcagatcgga aattttgtgg
       61 cagcctcgat tatggaaggc tcacagttgg atgccggctc ggaggccggc atgacgatga
      121 ttgggtcatt gcgggctcga cccgcaatct acctatgacg ttgcaacaca gcagggccgg
      181 acttcggacg aacgtattcg cgatagaatg ccgctttccg aggtctcgac tcggggggca
      241 tgtcgggaat tgggatggat tgcggcgcgg gcgggccgcg ggcggcgccg caatgacggc
      301 gcgctttagt ggcggcggag ttcgcggtag atgaaggtgg cggtggcaag gatgatgatc
      361 gcgccgatga agatgccgag gaacagcgag tagtcgtagt agtagccgtc gcgcaccggg
      421 tcgtaggtgg tgcagaaaag cttgacccgc gcgacgatgc cgtcgagcag cgtctgctgc
      481 ggcgccgggc ggccgagcac cagttcgatc agtggatcga ccagcagcgg ggtctcgaac
      541 acctggccgt aaacctggcg gtagaccgtg ccgtcggcat cgacgacgct cgcctggacc
      601 aggtggtcga agccgttcga cgtcgggtaa aagcgaaagc cgagatcttg cgacaacgca
      661 gcaaccgagt cggcatcgcc gctgaggata tgccacccct tgtccgcaac gccctggcgc
      721 gcggcgaagt agcgcattgc cgccgggtcg tcgaccgcgg tgtcgaagcc gatcagcgcg
      781 acgctgaaac tgtcctcgcc gagcgtggcg tgcgccttgt cgacgacctt cgcgagatgc
      841 tgcgtggtca tcgggcagat gtggtagcag ctggtgtaga tcatcgagat gacgagcggt
      901 ttgccgcgca ggctatcgag ggacaccgta cgaccgctag ttgcgtcgac gaaggcatga
      961 ttgccgacgc gcttgccgat cgctgcctgg ctcaccgcga gcgcggcgtc gttgtcgaag
     1021 gcggggtggg tcgtgtcggc agcgaaagtg acggatggga gtagtagcag caggacgagg
     1081 atatgggtgc gccatttggc aaggatcggg aacgaccggc gagccgcgag tgcaagatcc
     1141 ccgcttgcgc ggggatgact ccgaatctcg tcatgccggc ctccgagccg aggcgtcgga
     1201 ccgcggcatc cattgccgag tcggtgagga aatcggcccg ggcgacgccc ggggagaaat
     1261 tgcggctggt tgtaatggat tgcggctcga gggccgcaat gacgaggctt tggaattgcg
     1321 cgaggcggcg tctccgccag ggggccgcga ttacggggct ggggcaatgt aacggcctcg
     1381 aatcagccga gcagcagcac gtcgaggacg gcgccggcga gcagcaggac gagttgcagc
     1441 agcgaggcga ggaagctacg catcgcgacg cggggggacg ggtcgcgaat catgcggatg
     1501 ttgcgataga ggaacagccc gccaccggcg acggcgccgg cgaggtagat ccagccgagg
     1561 ccgaagaaga acggcattac cgacaccgcg accagcgcca gcgtgttggc gaacacgatc
     1621 tcggcggcgc gccgcttgcc gacgatcacc ggcagcatcg gcacgccggc tttctcgtag
     1681 tcggcgaact tggcgatcgc caggctccag aagtgcgacg gcgtccacag gaacagcgcc
     1741 agcgcgagcg cgagcggaat cgggcccggc agcgggtcga ccgcggccgc gccggcgagc
     1801 accgcgaagc tgccggcggc gccgccaacg acgatgttgg tccacgaacg gcgcttcagc
     1861 cagacggtgt agaccacggc gtagaagaac gcgccgagaa aaatatgcag cgcggccgcc
     1921 gcgttcagca gcagggccgc actgcccacc ccggcgacca gcagcccggc gatcgcgatc
     1981 agccacagcg ggccccggtg aaaataaccg gtcacgaacg gccgccggca ggtgcgtggc
     2041 atgcgccggt cgaggtcgac ctcgtagtaa tggttgaagg cgcccgcggc gcccgacgcg
     2101 atcagtactg ccaggaacag caccagggcg tcgacaaaac tcggccgcgg cccgggagtc
     2161 gcgagcatcg ccaccagcgc ggtcaaggcc atgatcacgc cgatgcgcag cttgagcagg
     2221 tcgacaagct gacgcgcgac ttcgcgcgtc gcaccgcgac cgccggggcg gccgaaaatc
     2281 agctcagcgg ccataccgag gccaggtaat cccagttgat gaagtagtag acgacgaagc
     2341 agaccagcag cgccatcgcc aacgcgaagg tgccgggcgc ctcgaaaccg gccgagccgt
     2401 gttgttcgac cgcgagcttc ggcgccatct gcagccgctc cggctgctcg acgttatacg
     2461 acgccggcct gagcggcacg ccgaaggatt gcagcaagcc ggccgattcg tcgagctttt
     2521 tgccgaacag cagcgaaccg accgtaacca gcaggaacat ggccccgccg agcaccgcca
     2581 gcacgccgcc gacgccggcc accgccatca tcgtgtatgc cgtgccgggg tactcaaagg
     2641 cgagcgccgc gccggtgaag tccatatccc agtggcgccg cgccacgccg agcgtaccgg
     2701 caccgagcat gaacaggatc atcagcgtca tgccggcgga gaacacgtag ggctgccacc
     2761 tggcgaccca cggcaagaac agttcgcgac ggaacagcac cgggatcagc aggtaggtca
     2821 gcgccatgaa ggccagcgtg gtgccgaccg cgaccgtggc gtggaaatgc gccggcacgt
     2881 agatcgtgtt gtgaatgatc aggttgatct gctcggtacc catgaccacg ccggtgatac
     2941 cgccgagaaa gccgaagatg atcagcgaga agaagaagcc cgagaacacc gggttgcccc
     3001 acggcgcctt gcgcaaccat tcgaacatac ccttggtgaa ccccttggcg cgttgcgcga
     3061 cttccatcga gccgggcacg gtgagcccgt ggatcatgct tgccagcacc gcgagataca
     3121 tcgcgtagct ggtgttgaag atcttgtact ccgcggacag tcccgggtcg accagtacat
     3181 ggtgcgcgct ggcgagctgc aaaaacagga tgtagagcag gaaggccata cggctgactt
     3241 tttcagacat cggccgcgcg ccgaacagca caccgacgat cagataccag atcgacacgt
     3301 gcgcggcgac gttgatctgc tgcgacgagt ggcccatccc ccaccagatg acgcggtaca
     3361 tctcggcgtc gatgtggttg atatagccga tcgaccacag gaaggtgggc accaggatga
     3421 tcgcgccgga ggcgacagtg aatatcgcga tgatgcacgc ggtcagcgca ccgaaggtga
     3481 ccagcgggat agagccctcg taggtgcggt cgtcgcgcgc gatcaccagc gtgccgagaa
     3541 acacgaagca gccgatcagc gcgccgaccg cgaacaggat caggccgagg tagaaatgcg
     3601 gtgccgcctg catcggcgcg taagacgtca tcatcacgct cgagttgccc tgcagcacgg
     3661 cgatattgtt ggtgatccag ccgatgatca tcaacgcgaa gccgagccac gcccagcgcg
     3721 gcgccgcgag ccgcgtgcgc agcagtgtcg acgacgcgaa gtagagaatc gccatctcga
     3781 agaagatgat ccagaagatc agcgcgttga gaccgtgacc ggtcagcgcc tggtagaaaa
     3841 ggtccgccgg cagcaggtgg acttcttgcc agcgggtgag gacgacgccg aggccgagga
     3901 tgccggcgac cagcagcgcc accgcgccgg ccaccgcgtt ggccttcatc aggctttcgg
     3961 cggacctgtc gaaatagaat cccgaatccg ggcaaaccct gaactgcgcc atgtcaattc
     4021 cccctcacgt agattttgcc aagcatcagg tggtgaccga tgccgcagta ctcgttgcag
     4081 accacggtga actcgccggc ctgatcgggc gccaccgtga gcaccatttc gtaaccgggc
     4141 actatctgca ggttgatatt gaccggctgc agcgaaaagc cgtgctgcca gtccatcgac
     4201 gacaggtgca gccgatacga ctgccccttt tgcagttcga gaatcgggta ccattgccac
     4261 agccggccga gcatgtagac gtcgctgccg gccggcggcc tgaccaccgg gatgcccgcc
     4321 tcgacgccga cctggtgagc ctcgaccatc gcatcgacgc gcgactggaa tttcgccggc
     4381 gtggtctggt aagcctcgct cgacaggttc tgttttccgt agacgtgcca gtagggcatc
     4441 atcaggaaca tgatcaggca ccagaccagc gcgatgccga tccagacgat ttcctgcttg
     4501 ccgaccggcg aactccacca gttacgctcc gcggggggta gaaatgaact cattggtttg
     4561 tctcccgggt actagggtgc gacggggatc gtggcgattt ccatgattcc ccacaggatg
     4621 taaaacaccg tgggcatgac gatgccgaga aacagcagca ggaacgggtt atcgagtacg
     4681 cgctgaaaaa acggcactcg ttcgggtttt gggatggtat cttgctcgga catggcaaac
     4741 ctcgcttcgt tgtgggtcga accccgcgca ttcgccaagt gcagcccttg tctcacttgc
     4801 acccgcccgg acagcccggc cacgaatggc gacagtttcg gcagatggca gattaatcaa
     4861 gcgatgccga aatgcccttg ataaaagtca aatcgcaaaa tcgcccgcgg agccccttga
     4921 taatggtcaa ttcgccggcg cgacgaccgt gctagcttgg cctggttacg gcaaaaaagg
     4981 acaggcttca tccatgacct cgatcgagtg gcgcaacgag tatcgcaccg gcatcgacgg
     5041 cgtcgactat gagcacgaga tcctgatcgg ccagatcaat acgctgctgc agatgatcgg
     5101 caaacgcgcc gatcgcgcgc tgatcctcga cggcctcggc gaaatctatg gcagcatcgc
     5161 ggcgcatttc gcgctcgagg aacaaaccat gcagaaactc ggctacgacg agtacgagcc
     5221 gcaccgcgca gaccacgagc ggctgctcga cgatatccgc gacctcgccg atgaattcga
     5281 aggctcggcc gagatcgacg aacagcagtt cggcgagcgc ctcgccagct ggttcggcca
     5341 tcatttccag acccacgatg cgcgcctgca ccgtctcgcc ggcgccaatg tccatcagcc
     5401 cgagagcggc tcggcgatgc gcaggatgat tcgcgatgcg aaacaaaaac tactcggcaa
     5461 gcatggttga gcggcacccc gcaagcacca ggagtcattc ccgcgcaagc gggaatctcc
     5521 agacacgccg ccatcgcaag atcctcgcta gcgcggggat gaccagggga aaagcgggga
     5581 tgactcccct ccgtcattcc cgcgcaagcg ggaatctcca gacacaccgc catcgcaaga
     5641 tccccgctag cgcggggatg accaggggaa aagtggggat cactagag
//
LOCUS       DATCZV010000288         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_341245, whole genome shotgun sequence.
ACCESSION   DATCZV010000288 DATCZV010000000
VERSION     DATCZV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341245"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIW27_06390"
     CDS             complement(<1..314)
                     /locus_tag="VIW27_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY5739336.1"
                     /translation="MSILINKDTRVVTQGITGKTGMFHTHHSIEYANGKQCYVAGVTP
                     KKGGQEVEGIPVFDTVAEAKAETGCNVSVIYVPPPFAAAAIDEAVDAEIEVVICITEG
                     IPV"
     gene            complement(317..1483)
                     /gene="sucC"
                     /locus_tag="VIW27_06395"
     CDS             complement(317..1483)
                     /gene="sucC"
                     /locus_tag="VIW27_06395"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5739337.1"
                     /translation="MKIHEYQGKEILREYDVAVPKGKACFSVDEAVAAAEEIGGSKWV
                     VKAQIHAGGRGKGGGVKLADSIDEVRELAGQILGMTLVTHQTGPEGKVVKRLLIEEGA
                     PIKDELYVGLVVDRATQRVTVMASSEGGTEIETVAEETPELIHKVMVDPQTGLTDAEA
                     QDLARKIGIPDALVGDAGKLMQSLYQAFWDKDCSLAEINPLIVTDDGRVVALDAKMNF
                     DESAMYRHPELQELRDLDEEDPDEIEASKFGLNYISLDGTIGCLVNGAGLAMATMDII
                     KLYGASPANFLDVGGGATTEQVTEAFKLMLKNKNLKAILVNIFGGIMRCDVIAEGLVN
                     AAREVDLGVPLVVRLEGTNVEQGRQILAASGISVISATTMADAAEKVAAAAAGA"
     gene            complement(1624..2634)
                     /locus_tag="VIW27_06400"
     CDS             complement(1624..2634)
                     /locus_tag="VIW27_06400"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY5739338.1"
                     /translation="MKVCVVGAGAIGGMMAVRLAQSGHELSVIARGKHLEAIQTNGLK
                     YLEKDEEVVITDLVATAEMSEITGQDLVLLAVKAHQIAPIADKLMPMLSDSGVIVTLQ
                     NGIPWWYFQKLAGEYEGNIVHTVDPDGSLAATIDPDRIIGCIAYPAATIDAPGVIRHI
                     EGHRFPLGELDGAESERVKTICDAFVEAGFKSFVLSDIRSEIWLKLWGNLSFNPISAL
                     THATLVDICQFPQTRALAAQMMTEAQQIAEKLGASFRVSLERRIEGAENVGKHKTSML
                     QDLEAGKALEIDGMLGVVIELGEQTATPTPAMNTVYALVSLLNKTTQQEGIRIAGRPH
                     DT"
     gene            3005..>3420
                     /locus_tag="VIW27_06405"
     CDS             3005..>3420
                     /locus_tag="VIW27_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5739339.1"
                     /translation="MTNWVRCQWQDRVCLGKLDGNRIEICEGSLFDDPVPSGETAQLA
                     EVRLLAPYTPTSILALWNNFHERAEAEGQTIPATPLYFMKPVTSLIGPGETIYRPRGH
                     QGRVIFEAELGIVIGRECRDIDAAEADEAIFGYTCV"
BASE COUNT          649 a         1072 c          979 g          720 t
ORIGIN      
        1 accggaatgc cctcggtgat gcagatcacg acctcaattt cggcgtcgac ggcctcgtcg
       61 atggccgccg cggcgaacgg cggcggcacg tatatcaccg aaacgttgca gccggtctcg
      121 gctttcgcct cggcaacggt atcgaaaacc ggaatcccct cgacttcctg gccgcccttt
      181 ttcggcgtca cgccggcaac atagcattgc ttgccgttgg cgtattcgat cgagtggtgg
      241 gtatggaaca taccggtctt accggtaatg ccctgggtaa ctacgcgagt gtctttgttg
      301 atgagtatcg acatgatcag gctcctgctg cggcggctgc cactttctcg gccgcgtcgg
      361 ccatggtggt ggcgctgatc accgaaattc ccgaagcggc gagaatctgg cgcccctgtt
      421 cgacgttggt gccctccagg cgcaccacca gcggcacgcc gagatcgacc tcgcgggccg
      481 cgttgaccag gccttcggcg atcacgtcgc aacgcatgat gccaccgaag atgttgacca
      541 ggatggcttt caggttcttg tttttaagca ttagcttgaa ggcctcggtg acctgctcgg
      601 tggtcgcacc gccgccgacg tcgagaaagt tagccggcga ggcgccgtag agcttgatga
      661 tgtccatggt cgccatcgcc aggccggcgc cattgaccag gcagccgatc gtgccatcca
      721 gcgagatgta gttgagaccg aatttcgagg cctcgatctc gtcgggatct tcctcgtcga
      781 ggtcgcgcag ttcctgtaat tcgggatgcc ggtacatcgc gctctcgtcg aagttcatct
      841 tcgcatccag cgcgaccacg cggccgtcat cggtgacgat cagcgggttg atctcggcca
      901 gcgagcaatc cttgtcccag aacgcctggt agagcgactg catcaacttg ccggcatcac
      961 cgaccagtgc gtcgggaata ccgatcttgc gcgccagatc ctgcgcttcg gcatcggtca
     1021 gtccggtctg cggatcgacc atgaccttgt gaatcagttc gggggtttct tcggcgacag
     1081 tttcgatctc ggtgccaccc tcggaactgg ccatcacggt aacgcgctgc gtcgcgcgat
     1141 cgaccaccag gccgacgtaa agctcgtcct tgatcggcgc cccttcctcg atcagcaggc
     1201 gcttgacaac cttgccctcg ggaccggtct ggtgggtaac cagcgtcatg ccgagaattt
     1261 gaccagccag ttcgcgcacc tcgtcaatac tgtcggcgag cttgacgccg ccgcccttac
     1321 cacggccacc ggcatgaatc tgcgccttga cgacccattt gctgccgccg atttcctcgg
     1381 ccgcggcgac cgcttcgtcg accgaaaaac acgccttgcc ctttggcacg gcgacgtcat
     1441 actcgcgcaa gatttccttg ccctggtact cgtggatttt cattattgcc tcaccccgcc
     1501 tgtaaaaaat ctggtatatc gtataccaga ttaattcttt tacaaaaaaa acgcgccaat
     1561 ttgtcgatcg acgcgcctcg aataatgtca gtttggcgcc gctgaagcaa aacggcgatc
     1621 gccctaggta tcatgtggtc gcccggcaat acgaattccc tcttgctggg tcgtcttgtt
     1681 gagcaggctg accagcgcgt agaccgtgtt catcgccggc gtcggtgtag cggtctgttc
     1741 gccgagctcg ataaccacgc cgagcatgcc gtcgatttcc agcgccttgc cggcctcgag
     1801 atcctgcagc atcgaagtct tgtgcttgcc aacgttctcg gcgccttcga tgcggcgttc
     1861 cagcgaaaca cgaaaactgg cgcccagttt ttcggcgatc tgttgcgcct cggtcatcat
     1921 ctgcgccgcc agcgcgcggg tctgcggaaa ctggcagata tcgaccagcg tcgcgtgggt
     1981 cagcgcgctg atcgggttga acgacaggtt gccccacagc ttgagccaga tttccgagcg
     2041 tatatctgac agtacgaagg acttgaagcc ggcctcgaca aatgcgtcgc agatcgtctt
     2101 cacgcgttcg ctctcggcac cgtcgagttc gccgagcgga aagcggtgac cctcgatatg
     2161 ccgaatcacg ccgggcgcgt cgatggtcgc cgccgggtag gcgatacagc cgatgatacg
     2221 gtcgggatcg atggtggccg cgagcgagcc gtcggggtcg acggtatgca cgatattgcc
     2281 ctcgtattcg ccggcgagct tctggaaata ccaccacgga ataccgtttt gcagcgtgac
     2341 aatgacgccg ctgtccgaca gcatcggcat cagtttatcg gcgatcggcg cgatctggtg
     2401 agccttgacg gctagcaaca ccagatcctg gccggtgatt tcgctcatct cggcggtcgc
     2461 gacgaggtca gtgatgacca cttcctcgtc cttttctagg tacttcaggc cgttggtctg
     2521 gatcgcctcg agatgcttgc cgcgggcaat aaccgacagt tcgtgacccg actgggccag
     2581 cctgaccgcc atcatgccgc cgatcgcacc ggctccgacc acgcaaacct tcatgcctaa
     2641 ccccgttaaa tcgataaaaa aaaccggcgc attctacgcc attaacggcg tgcaagtcca
     2701 cgcgccggac taattgttgc tattaatttt cagggctcaa cagcgcctgc ctcgaccgtg
     2761 cgggtaacga atcgaccccg tctcccgggc tgcgcgacaa ccgtgcttat cgcattcctc
     2821 cagccctcga aacgggcaac actatacccg ttaaccccgg ccgacggcga atcaataaag
     2881 tgtatgaggg taaacaggaa ttgcgccctg cccctgaatc ggtatcggcg gcaggacaag
     2941 gtaatttgag tttcatcccc attgtgataa tttcgcgccc ttcagacaat caggaccaca
     3001 tctcatgacc aactgggttc gctgccaatg gcaggaccgg gtttgcctcg gcaaactcga
     3061 cggcaatcgt atcgagattt gcgaggggtc gctgttcgac gaccctgtcc cgagcggtga
     3121 aaccgcgcag cttgccgagg taaggctgct cgcgccttac acgcccactt cgattcttgc
     3181 gctgtggaac aatttccacg agcgcgccga ggcggagggc cagacgatac cggcgacgcc
     3241 gctttacttc atgaaaccgg tgaccagcct gatcggcccg ggcgaaacca tttaccggcc
     3301 gcgcggtcat cagggacggg tgatcttcga ggccgagctc ggcatcgtca tcggccgcga
     3361 gtgccgcgat atcgacgccg ccgaggccga cgaggcgatc ttcggctaca cctgcgtcaa
//
LOCUS       DATCZV010000289         4846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_711755, whole genome shotgun sequence.
ACCESSION   DATCZV010000289 DATCZV010000000
VERSION     DATCZV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711755"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="lptF"
                     /locus_tag="VIW27_06410"
     CDS             complement(<1..995)
                     /gene="lptF"
                     /locus_tag="VIW27_06410"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY5739340.1"
                     /translation="MVNIITAYLAREILKASMATLLILYVILVSNALGRVLADIADGD
                     IPARALWPVLLSHSMNILTLLLPVGVFLGIVFAFGRMYKDHEIVVMNACGIGYLDFYK
                     PVAIVLLPIFAFSVYSSLWMNAQVQRDAQAIIDREKGRHEFEQLKPGQFNQSKSGGLV
                     FFMESISADRLELRNVIISELGSDRRVLETADTGRQKLEESTGNLFLVIGPGERYEGE
                     PGAVAHHVIEYDQHGILIENQAGSASSRLRNEQLSPAELWNSPTLAAQIELYWRISTP
                     MVLLVLGFIAVPLAYIAPRQGRYGKMGYALLIYIAYLNLMVIARAKLADETLPLV"
     gene            1140..2630
                     /locus_tag="VIW27_06415"
     CDS             1140..2630
                     /locus_tag="VIW27_06415"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY5739341.1"
                     /translation="MDFSIKHHAAENCRGACQVITVWQPRKLSASGQSVDQASKGYLS
                     AILKNGDMDGKIGQTLMLPGVPGVAAKRVLLVGCGKRSEMDERAYGRAINAIASALAA
                     SGARDAELYPADIELKGRDTAWKVRQAALLLANSEYRFEQCKSGKKQPRMALRKIHFN
                     VDRKDAASATAAFEEASAISAGMSLARDLGNLPANICTPSYLASQARQLARGVKSLRV
                     TVLGEPEMKKLKMGSLLSVSAGSRQPARLIVIEYRGAKASEAPVALVGKGVTFDTGGI
                     SLKPGQAMDEMKYDMCGAASVFGTIKAVNQLQLPINVVGIVPATENMPGGLATKPGDI
                     VTSMSGQTIEILNTDAEGRLILCDALTYAGKYKPRSVIDIATLTGAVIVALGKIPSGV
                     MGNDRKLIDELRACGDQIHDRIWELPLWDDYQEQLKSNFADMANIGGRDAGTITAACF
                     LSRFTRDYQWAHLDIAGTAWKSGDDKGATGRPVPLLTQYILNQVKR"
     gene            2627..3040
                     /locus_tag="VIW27_06420"
     CDS             2627..3040
                     /locus_tag="VIW27_06420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY5739342.1"
                     /translation="MTRIDFYELQPNRHRLDQIVCQLCQKAYDHGQLTLVLTSDPEQS
                     RHLDEKLWTYRDDSFLPHDIDEPDGLVTPVLINDRPEPRGHRQLLINLAAEVPDFFAQ
                     FDRVIELVTDDNRQSARGHYSYYKERGYPLEHISL"
     gene            3037..3318
                     /locus_tag="VIW27_06425"
     CDS             3037..3318
                     /locus_tag="VIW27_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739343.1"
                     /translation="MSTEDDIPTLTDLIEKRGGDDEIRIADLGLDDADLGLGGVDIEV
                     EAFPSPRPAGDPLRDNPALEQTVRRILDEHMELAWQEIKLAIQQEFDKS"
     gene            3633..>4846
                     /gene="valS"
                     /locus_tag="VIW27_06430"
     CDS             3633..>4846
                     /gene="valS"
                     /locus_tag="VIW27_06430"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY5739344.1"
                     /translation="MDKTYDPHAIEQTWYQRWEEKGYFAASGDGAPYCIVIPPPNVTG
                     SLHMGHAFQDTIMDTLIRYHRMKGDATLWQPGTDHAGIATQMVVERQLSQQGKSRHEV
                     GRDAFIERVWDWKEHSGGTITRQLRRMGASPDWSRERFTMDDGLSAAVQEVFIRLYEE
                     GLIYRGSRLVNWDPVLHTAISDLEVVNVEEKGHLYHVRYPIVGEPIDGVTHMEIATTR
                     PETILADGALAVAPGDERYAKFVGRQVQVPMTTRIIPIITDDYVDSEFGTGCVKITPA
                     HDFNDYEVGQRHEMEVINLFTIDAIMNDNAPPAYRGMDRFAARDKIVKDLDEADLLVK
                     VEDHVYKRPYGDRSHVVIEPYMTDQWFVRAEPLAKPSIEAVRDGRIRFIPQNWSKTYY
                     NWMEDIQDWCISR"
BASE COUNT         1043 a         1512 c         1422 g          869 t
ORIGIN      
        1 attaccagcg gcagcgtttc gtcggccagc ttggcgcgcg cgatcaccat caggttcagg
       61 taggcaatgt agatcagcaa tgcataaccc atcttgccgt agcgcccctg ccgcggcgca
      121 atgtatgcca gcggtacggc gatgaatccg agcaccagca acaccatcgg cgtcgagatg
      181 cgccagtaaa gttcgatctg cgccgcgagc gtcggcgagt tccacaactc ggccggggac
      241 agctgctcgt tgcgcagtcg gctggatgcg gaaccggcct ggttctcgat cagtataccg
      301 tgttgatcgt actcgatgac atggtgggcg accgcgccgg gctcgccctc gtagcgctcg
      361 ccggggccga taaccaggaa caggttaccg gtgctttcct cgagcttttg tcgtcccgta
      421 tcggcggttt ccagcacccg tctgtcgctg cccagttcgc tgatgatgac attgcgtaat
      481 tcgaggcgat ccgcgctaat cgactccatg aaaaatacca gtcccccgct cttgctctgg
      541 ttgaattgcc ccggcttgag ctgctcgaat tcgtgccggc ccttttcacg atcgatgatt
      601 gcctgtgcat cgcgttgcac ctgggcattc atccacaggc tcgaataaac gctgaaagcg
      661 aagatcggca gtaacacgat ggccaccggc ttgtagaaat cgaggtaacc gataccgcag
      721 gcattcatga cgacgatttc gtgatccttg tacatgcggc cgaacgcaaa cacgatgccg
      781 aggaacacgc ccaccggcag cagcagggtg agaatattca tcgagtgact gagcagcacg
      841 ggccacaggg cacgcgccgg aatatcgccg tcggcaatat cggcaagtac gcggccgagg
      901 gcgttgctga ccaggatgac gtagagtatc agcagcgtcg ccatgctggc cttcaaaatt
      961 tcgcgcgcca gataagcggt gatgatattg accattgacg attccgagtt gagcgcttcg
     1021 cgcaccagac tagccggcgt cagcttaaag caggctgaat tttttgcctt cacggctggt
     1081 ttttttcggc gcttcacgta tcttatcgcg ttatcttctc gttcgacacc aggaatatca
     1141 tggacttcag tatcaaacac cacgctgccg agaactgccg cggcgcctgc caggtgataa
     1201 ctgtttggca accgaggaaa ctgtccgcca gcggccaaag cgtggaccag gcgagcaagg
     1261 gttacctgag cgccatcctc aagaacggcg acatggacgg caagatcggc cagaccctga
     1321 tgttgcccgg ggtaccgggc gtcgccgcga aacgggtgct gctggttggc tgcggcaagc
     1381 gcagcgagat ggacgagcgt gcctatggcc gcgccattaa tgcgatcgcc tccgcgctcg
     1441 ccgcttccgg ggcccgcgac gccgaactct accccgccga catcgagctc aaggggcgcg
     1501 acacggcgtg gaaggttcgt caggcggccc tgctgctggc taacagcgaa tatcgtttcg
     1561 aacagtgcaa gagtggtaag aaacagccac gcatggcgtt gcgcaagatc catttcaacg
     1621 tcgaccgcaa ggacgccgcg agcgccacgg ctgctttcga agaggccagc gcgatcagcg
     1681 ccggcatgag cctggctcgc gacctcggca acctgccggc caatatctgt acccccagtt
     1741 atctcgccag ccaggcgcgg caactggcca ggggcgtgaa aagcctgcgc gtgacggtgc
     1801 tcggcgagcc ggaaatgaaa aaactgaaga tgggctcgct gctgtcggtc agcgccggca
     1861 gccgccagcc ggcgcggctg atcgtcatcg aataccgcgg tgccaaggcg agcgaagcgc
     1921 cggtggcgct ggtcggcaag ggtgtgacct tcgataccgg cggcatttcg ctgaaacccg
     1981 gccaggcgat ggacgaaatg aaatacgata tgtgcggcgc ggcctcggta ttcggcacca
     2041 tcaaggcggt caaccagctg cagctgccga tcaacgtggt tggcatcgta ccggccaccg
     2101 agaatatgcc cggaggtctc gccaccaagc ccggcgacat cgtcaccagc atgtccggac
     2161 aaaccatcga aattctgaat accgatgccg agggacgcct gatcctgtgc gacgcactga
     2221 cctatgccgg caagtacaaa ccgcgcagcg tgatcgatat cgccacactg accggcgcgg
     2281 tgatcgtggc gctcggcaag attccctccg gcgtgatggg caacgaccgc aagctgatcg
     2341 acgaactgcg cgcctgcggc gatcaaatcc acgaccggat ctgggaattg ccgctttggg
     2401 acgattacca ggaacagttg aagagcaatt tcgccgacat ggcgaatatc ggcggccgtg
     2461 acgccggcac gattaccgcc gcctgtttcc tgtcccgctt tacccgcgac taccagtggg
     2521 cgcatctcga tatcgccggt acggcgtgga aatcgggcga cgacaagggc gccaccgggc
     2581 gcccggtgcc gctactgacg cagtacatcc tgaaccaggt caagcgatga cgcgtatcga
     2641 cttttacgag ctgcagccga atcgacatcg cttagatcaa atcgtctgcc agctgtgcca
     2701 gaaagcttac gatcacggtc aactaaccct ggtactgacc agcgaccccg agcaaagccg
     2761 ccatcttgac gagaaactct ggacctatcg cgacgacagt ttcctgccgc acgacatcga
     2821 cgagcccgac ggactggtga ccccagtcct gatcaacgac aggcccgagc cacgcggcca
     2881 tcgacagctg ctgatcaacc tggcggccga agtaccggat ttttttgccc agttcgatcg
     2941 cgtcatcgaa ctggtgaccg acgacaaccg tcagagcgcc cgcggacatt acagctatta
     3001 caaggagcgc ggctacccgc tggagcacat cagcctgtga gcaccgagga cgatattccg
     3061 acgcttaccg acctgatcga aaagcgcggc ggcgacgacg aaatccgcat cgccgatctc
     3121 ggtctcgacg acgccgacct cggccttggc ggtgtagaca tcgaggttga agcttttccc
     3181 tcgccgcgtc ccgcgggcga cccgttgcgc gacaacccgg cgctcgagca gaccgtgcgc
     3241 cgcattctcg acgaacacat ggaactcgcc tggcaggaaa ttaaactcgc catccagcaa
     3301 gaattcgaca agtcctgaag gtcggggtca gatcaaaaaa tttcagaaac gcgttgactg
     3361 acaccttcga aagccggcga aatctttgct tgggcaaaga tttcctcagg cttcgctcga
     3421 ggtcgcgccg atcagggaat tattgctctg gcccctctag gtgggtctgc attcaatcgg
     3481 tgctcccgat gcaagcccat gctagcgggc ttgccgaagc cattagcttc ccgaacagcc
     3541 aaatcccgaa tcccggttca gcaaactgaa cattcctgta gccggaaacc caaggtataa
     3601 tcgcgccctt tgaatcgacg ccgcgaccct gaatggacaa gacttacgat cctcacgcga
     3661 ttgagcagac ctggtaccag cgctgggaag aaaaaggtta tttcgccgcc agcggcgatg
     3721 gcgcgcccta ctgcatcgtg attccgccgc cgaatgtcac cggcagcctg cacatggggc
     3781 acgcgttcca ggatacgatc atggatacgc tgatccgtta ccatcgcatg aagggagatg
     3841 ccacgctgtg gcagcccggc accgaccacg ccggcatcgc cacgcaaatg gtggtcgagc
     3901 gccagctcag ccagcagggc aagagccgcc acgaagtcgg tcgcgacgcc ttcatcgaac
     3961 gcgtctggga ctggaaggag cattcaggcg gcaccatcac ccgccagctg cggcgcatgg
     4021 gcgcctcgcc cgactggtca cgcgaacgct tcacgatgga tgacggcctg tcagcggccg
     4081 tacaggaagt ctttattcga ctctacgagg aaggcctgat ctaccgcggc agccgtctgg
     4141 tcaactggga cccggtactg cataccgcga tttccgatct cgaggtcgtc aacgtcgagg
     4201 aaaagggaca cctctaccat gtacgttacc cgatcgtcgg cgagccgatc gacggcgtta
     4261 cccacatgga gatcgcgacc acgcggccgg aaaccatcct cgccgacggc gcgctcgcgg
     4321 tcgcccccgg cgacgagcgc tacgcgaagt tcgtcggccg ccaggtacag gtgccgatga
     4381 cgacgcgcat catcccgatc atcaccgatg actacgtcga ctccgagttc ggcaccggct
     4441 gtgtcaagat caccccggcg cacgacttca acgactatga ggtcggtcag cgccacgaga
     4501 tggaagtcat caacctgttc accatcgacg cgatcatgaa cgacaatgcg ccgccggcct
     4561 accgcggtat ggatcgtttc gcggcgcgcg acaagatcgt caaggatctc gatgaagccg
     4621 acctgctggt caaggtcgag gaccacgtct acaagcgccc ctacggcgac cgctcgcacg
     4681 tggtcatcga accctatatg accgaccagt ggttcgtgcg tgccgagccg ctggcgaagc
     4741 cgtcgatcga ggcggttcgc gacggtcgca tcaggttcat tccgcagaac tggagcaaga
     4801 cttattacaa ctggatggaa gacatccagg actggtgcat ctcgcg
//
LOCUS       DATCZV010000290         8993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_235167, whole genome shotgun sequence.
ACCESSION   DATCZV010000290 DATCZV010000000
VERSION     DATCZV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235167"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3376)
                     /locus_tag="VIW27_06435"
     CDS             complement(<1..3376)
                     /locus_tag="VIW27_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739345.1"
                     /translation="METTPSQTAEHPKAGPPASLLARRGVPATVEAIVARLRRRWKLR
                     LLLQGLAVSLALGGLLFIAAAWLLNLWHFSAPAVWSLRLLTLAVCVALLLRYCVMPLR
                     RRIADSRVALYLEEHEPSLRGLLSSAVDARRLPADSVSPQLAARLAEQAVDACRKLHF
                     GDSAEARKLGAAVFRLGLVVLAAALLVLWPPQFLQFGAPALLQPWTDASEYSPYRIEL
                     TPGDIEIAHGADQLISASITGYDGGDILLFTSRDGGRSWQQQSMAAGNARGVYESFLF
                     DLDSDLDYYVAGAGQQTPTYRIAVAQIPVIEQIGLRFHYPAYTMLPAETRFGNGDIRA
                     LEGTRVEVLIEPSIEIPGGELLFDDGRSVALEPVANGGWVGELVVQQAATYRVRLQRA
                     SGALVDASPEYRIAVIDDQHPSVSILSPGRDTKVSMVEEPLMRIRASDDQGIADLQLV
                     MSVNGAAEKSIDLLPSDGGENRQQIEAEHVIYLEDLGLQPGDLISYYVRARELAPETQ
                     HKTATSDIFFYQVRPFSMNYRSAAERGGGGGGGQQGGEQQGHLSEQQKQFVVATFKMI
                     RDRDSYQQETWNENLELLANAEARIRDRVEAIVRRIGRRPIVQSDERYQVVMRELPLA
                     TEAMIEVENLLRQAEIETALSDAQRALLHLQRADAAFREINVSLASQGGGGGGNSGSN
                     ELADLFQLEMDKLRNQYETVQRGQQQAPQQVIDEALERLRELAQRQQREVERQIRRLD
                     QGLGSEAASRQRALAEELEEMARQLERLTREQHSQRLQQSIAQMRKAAESMRRAAANA
                     EAGSGASDAQAAEESLREARRLLDQGRAQQISEAVERSLRRAELAEKKQAVIKREVAS
                     IDDPPGSDLEQKLDRLQQRKQSLSDELAHLESELGELTGEAREEQPEAAEALKQALES
                     GHDNRVQDRIGRTRRMMELGARKNALQNEEHIQRSIGRMREHIEEALAQIGEPAERGL
                     QRSLEQMRDLARELRFSRERAQNAADGERQAPANQASADGNRQAAENSASAGAGDRDR
                     QTQGNSTPTGGAANGGYADGSSGYAVTGVQPLRRNLDSIAKRTRELGHRLARQGVDSG
                     DIEPVLARIDELTRASNLADSAALHDDALLALMELEY"
     gene            complement(3390..5474)
                     /locus_tag="VIW27_06440"
     CDS             complement(3390..5474)
                     /locus_tag="VIW27_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEY5739346.1"
                     /translation="MALAWINPLYLAGLLLLALPVLIHLVQRRHSQGLKFPSLMFLRQ
                     IPWREKRRLEIRHWLLLLLRCLLLALLALAFARPFFDSGAAVMPNTERSDGVIVIDRS
                     YSMRIGERWDEARQLALGLVDTKRPGDRLGVIAFDADTELASDLTDNPDDLRGAIRRQ
                     QPGLRGTRLRAAVEQAARLLEGSNAVRRRILLISDFQAAAGEIPRIDDGIEVEALAVN
                     AAAADNAAIAALVIEAPADGAVDEFGLAVEIVNHGEREREQRLVLSLDGRQLPPRILQ
                     LAAGETRVVRFDGLTPGAGLLRGVASLDSDALDIDNHAHFVYSDQQRVPVLIVEGETP
                     RANQSLYLERALGLSRQPLFSVRRSSWQALEDKPLSAWSVIIVNDAPIPGGELGAALE
                     DFVAAGGGLLVALGGAVQGNWPSGASGLLPGVLAPRIDAVAGNAQRIGALDRTHPLFA
                     ADAPGIDLSGARIYSYRGLETGERDRVLARYDDGGAALLEKRFGDGRVLVLTSTLDAY
                     WNDFALRPAFLPFLHQALRYLAEYEAYANRFEIGAIVDVLRHARALTGTDAIVAASGS
                     ETLVVEAPSDREIRLRRDSALLKIDEQGFYQVHRATPAGVDVVLAADVDSAESVPQAL
                     DVKQFVEEIRDAATAAPAARVVTRRQASEYEQRQQIWYKLLLAALVLALLEALFANRI
                     AAGRFARKPAGT"
     gene            complement(5474..6397)
                     /locus_tag="VIW27_06445"
     CDS             complement(5474..6397)
                     /locus_tag="VIW27_06445"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5739347.1"
                     /translation="MLGAKTTQAQLPGQRFIDPAVLTRIDNLELIARRVVDGFISGLH
                     QSPMLGMSVDFAEHRVYMPGDDVRRIDWRLFARTDRLYVKEFEADTNANLHLLLDVSK
                     SMDFGSGGVTKLEYAKYLAASLAYLSTRQRDRIGLVTFSDDIVDTIPPSIRHFNLVLH
                     ALDRVQAEGVSNYHRPLQRMTESLRRRSLVVLISDLYEPPESLLPAINQLRYRGNDLI
                     VFHLLDPQELNLELGDVSSVEELETGVDLPISTREFRDRYQQLVNEHRAELERRLGGN
                     QIDYIFTDISQPLDGVLYRYLSDRERRKRTR"
     gene            complement(6409..7446)
                     /locus_tag="VIW27_06450"
     CDS             complement(6409..7446)
                     /locus_tag="VIW27_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5739348.1"
                     /translation="MDIETEQATTLQGEDDIRLAEDLRARYGRAREELAKLIVGQHSV
                     VEQVLITILAGGNSLIVGVPGLAKTLLIHSIAQVLDLKFSRIQFTPDLMPSDITGTDL
                     VQENPDSGKRELVFLPGPIFANIVLADEINRTPPKTQSALLEAMQEHRVTVQGKTYDL
                     EEPFFVFATQNPIELEGTYPLPEAQQDRFMFEIVIDYLSAADELEVVRQTTSLRDIKF
                     EYAMNGEQIRAFQKLVRRVPVSRAVSQYALDLVRCSRPGSDAAPAFVNDWVAYGASTR
                     AAQNLVLGGKTRALIDGRYHVSFDDIAALAKPVLRHRVLRNFHAESEKIGSDEIIDRL
                     LEAVPVPESGM"
     gene            complement(7493..>8993)
                     /locus_tag="VIW27_06455"
     CDS             complement(7493..>8993)
                     /locus_tag="VIW27_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739349.1"
                     /translation="RRGAMVEGKENLERSFAGDPYNVWIKNTLQLADSFENYAETDRG
                     KFRVVLHDNEQALLGEYVHEIAEQAYEAFAERYDYRPQAPIRVEFYPNHADFSVRTVG
                     LAGVGLLGVAFGPVVAMDSPAARERGAFNWGSTLWHELAHVFHLTMTDNRVPRWFSEG
                     LAVYEERQGRPGWGGDVDPGFLRAYIAGQLLPLSQLNSGFVRPSYPEQVVHSYFQASL
                     VFDFIESRRGFDAIREALNAYRDSSDPDAVLPAVLELDAAALDAAFDEYFRKRYAVPL
                     AALQGTVDELDPGETESGPSIDNAQPDRYFDQLRLGISLYDKGDLELAELFLTRAQQL
                     FPQYAGGDSSYWLLASLYQQQNRPDAAEEQLARMTAINAEHYAAQRQLASLRAARGDD
                     AGAAEALQSAIYIYPYDPDLHHALAGHLEKLERWQAVARERRAILALDPIDRAEAHYQ
                     LARAYQRAGNRSSAREQILYALEIAPNYFQAQELLLALHQVGATVQPPP"
BASE COUNT         1397 a         2935 c         3032 g         1629 t
ORIGIN      
        1 ggtattcgag ttccatcagc gcgagcagcg cgtcgtcgtg cagtgcggcg ctgtcggcca
       61 gattcgaagc ccgggtgagt tcgtcgatcc tcgcgagtac cggctcgata tcgccgctgt
      121 cgacaccctg cctggccagc cggtgaccga gctcgcgggt gcgtttggcg atgctgtcca
      181 gattgcgcct cagcggttgc acgccggtca ccgcgtagcc gctggagccg tcggcgtagc
      241 caccgttggc ggcaccgccg gtgggggtcg aatttccctg tgtctgccga tcgcggtcgc
      301 cagcaccggc ggacgcggaa ttctcggcgg cctgccgatt gccatcggcg gatgcctgat
      361 tcgcgggcgc ctgccgctcg ccatcggcgg cgttttgcgc gcgttcccgc gaaaagcgca
      421 attcccgcgc caggtcgcgc atttgctcga gcgaacgttg caggccacgt tcggcgggtt
      481 cgccgatctg tgccagcgct tcctcgatat gctcgcgcat gcggccgatc gaacgctgaa
      541 tatgttcctc gttttgcagc gcgtttttgc gcgcaccgag ctccatcatc ctgcgcgtgc
      601 gcccgatgcg gtcctgcacg cgattgtcgt ggcccgattc gagcgcttgt ttgagcgctt
      661 cggcggcctc cggctgctcc tcgcgcgcct cgccggtcag ctccccgagc tcgctttcga
      721 ggtgggcgag ttcgtcggac aggctttgct tgcgctgctg caggcgatcg agcttttgct
      781 cgagatcact gccgggcgga tcgtcgatcg aagccacctc gcgcttgatt acggcctgtt
      841 ttttctctgc cagttcggcg cgccgcaggc tgcgttcgac cgcctcgctg atttgctgcg
      901 cacggccctg gtcgagcagg cggcgcgcct cgcgcaggct ttcctcggcc gcttgcgcat
      961 cgctcgcacc gctgccggcc tcggcgttgg ctgcggcgcg gcgcatcgac tcggcggcct
     1021 tgcgcatctg tgcgatcgat tgctgcagcc gctggctgtg ctgttcgcgg gtcaggcgct
     1081 ccagttgccg tgccatttcc tcgagttcct cggccaacgc tcgctggcgg ctggccgcct
     1141 cgctgccgag gccctggtcg aggcggcgaa tctggcgctc gacctcgcgc tgctggcgtt
     1201 gcgcgagttc gcgcaggcgc tcgagagctt catcgatgac ctgctgcggc gcctgttgct
     1261 ggccgcgctg cacggtttcg tactggttgc gcagcttgtc catttccagc tggaacaggt
     1321 cggccagttc gttggaaccg ctgttgccgc cacccccgcc gccctggctg gccagcgata
     1381 cgttgatctc acgaaatgcg gcgtcggcgc gctgcaggtg taacagcgcc cgctgcgcat
     1441 cgctcagcgc ggtttcgatc tcggcctgtc gtagcagatt ttcgacctcg atcatggctt
     1501 ccgtggcgag cggcagctcc cgcatcacca cctggtagcg ctcgtcggat tgcacgatcg
     1561 gccggcggcc gatacgccgc acgatggcct cgacgcgatc gcggatccgc gcttccgcgt
     1621 tggccagcaa ctccaggttt tcgttccagg tctcctgttg atagctgtcg cgatcgcgga
     1681 tcatcttgaa ggtcgcgacc acgaactgct tttgttgctc ggacaggtgg ccctgctgct
     1741 caccgccctg ctggccgccg ccaccgccgc cgccgcgttc tgcggcgctg cgataattca
     1801 tgctgaacgg gcgtacctgg tagaaaaaga tatcgctggt cgccgtctta tgctgtgttt
     1861 ccggagccag ctcgcgggcc ctgacgtaat acgaaatcag gtcacccggt tgcagcccca
     1921 ggtcctcgag gtaaatcacg tgctcggcct cgatttgctg ccggttttcg ccgccgtcgg
     1981 acggcagcag gtcgatcgac ttctcggctg cgccgttgac ggacatcacc agttgcaggt
     2041 cggcaatacc ctgatcgtcg ctggcacgga tgcgcatcaa cggctcttcg accatgctga
     2101 ccttggtgtc gcggccggga ctgaggatcg aaacgctggg atgctggtcg tcgatcaccg
     2161 cgatgcggta ctcgggcgag gcatcgacca gcgcaccgct ggcgcgctgc aggcgcacgc
     2221 ggtaagtggc cgcctgctgc acgacgagtt cgccgaccca gccgccgttg gcgaccggct
     2281 cgagggcgac gctgcgcccg tcgtcgaaca gcaactcgcc gcccgggatc tcgatgctgg
     2341 gctcgatcag gacttcgacg cgggtcccct cgagggcacg aatatcgcca ttgccgaagc
     2401 gggtttcggc gggcagcatg gtataggccg ggtaatgaaa gcgcaggccg atttgctcga
     2461 tcaccgggat ttgggcgacc gcgatgcggt aggtcggggt ctgctggccg gcgccggcga
     2521 cgtagtagtc gaggtcgctg tcgaggtcga aaagaaagct ttcgtagacg cccctggcgt
     2581 tgccggcagc catgctctgc tgttgccagg aacgcccgcc gtcgcgcgag gtgaacagca
     2641 gtatatcgcc gccgtcgtag ccggtgatgc tggcgctgat caactggtcg gcgccgtgcg
     2701 cgatctcgat gtcgccgggt gtaagctcta ttcgataggg cgaatactca ctggcgtcgg
     2761 tccacggctg cagcagtgcc ggcgcgccga attgcaggaa ctgcggcggc cacagcacca
     2821 gcagcgccgc cgccagtact accaggccga gacggaaaac cgccgcgccc agcttgcgcg
     2881 cctcggcgct gtcgccgaag tgcagtttgc ggcaggcatc gacggcctgc tcggcgaggc
     2941 gcgcggcgag ctgcggcgaa acggagtcgg ccggcaggcg gcgcgcgtcg actgcgctcg
     3001 acagcagccc gcgcagcgag ggttcgtgtt cttccaggta cagcgctacc cgcgagtcgg
     3061 cgattcgccg gcgtagcggc attacgcaat agcgcagcag cagcgcgacg cacacggcca
     3121 gtgtcagcag gcgcagactc cagaccgccg gcgcgctgaa atgccagaga ttcaacagcc
     3181 atgcggcggc gatgaaaagc aggccgccga gcgccagcga aacggccagt ccctgcagca
     3241 gcaggcgcaa tttccagcgg cgccgcaggc gcgcgacgat cgcctcgacg gttgccggaa
     3301 cgccgcgccg cgccagcagc gaggcgggcg gcccggcctt tggatgctct gcggtttggc
     3361 ttggcgttgt ctccataatc cgtgtccttt caggtccctg ccggcttgcg cgcgaatcgc
     3421 ccggcggcga tacggttggc aaacaaggcc tcgagcagcg cgagtaccag tgccgccagc
     3481 aacagcttgt accagatctg ctggcgctgc tcgtactcgc tggcctggcg ccgggtcacc
     3541 acccgtgccg ccggcgcggc ggtcgccgcg tccctaattt cctccacaaa ttgcttcacg
     3601 tcaagcgcct gcggaaccga ttcggcgctg tcgacgtccg ccgccagcac tacgtcgacc
     3661 ccggcaggag tggcgcggtg cacctggtaa aagccctgtt cgtcgatttt cagcagtgcc
     3721 gaatcgcgcc gcaggcgaat ttcccggtcc gacggggctt cgacgaccag tgtctcgctg
     3781 ccggacgcgg cgacgatcgc atcggtgccg gtcagcgccc gcgcatggcg cagcacgtcg
     3841 acgatagcgc cgatctcgaa gcggttcgcg taagcctcgt attcggccag gtagcgcagc
     3901 gcctggtgca aaaacggcag gaaagctggc cgcaacgcga aatcgttcca gtaggcgtca
     3961 agcgtcgaag tcagcaccag cacccggccg tcgccgaagc gcttttccag cagcgcggcg
     4021 ccgccgtcgt cgtagcgggc cagcacccgg tcgcgctcgc cggtttcgag accgcggtag
     4081 ctgtagatgc gcgcgcccga caggtcgatg ccgggcgcgt cggcggcaaa cagcggatgc
     4141 gtgcgatcca gcgcgccgat gcgctgtgcg ttgcccgcca ccgcgtcgat ccgcggcgcc
     4201 agtaccccgg gcagcaggcc gctcgcgccg gatggccagt tgccctgcac cgcgccgccg
     4261 agggccacca gcaggccgcc gccggcggcg acgaaatcct cgagtgcggc gccgagttcg
     4321 ccaccgggaa tcggtgcgtc gttgacgatg attaccgacc aggccgacag cggcttgtcc
     4381 tcgagggcct gccaggaact gcgccgcacg ctgaacagcg gctggcgaga cagtccgagt
     4441 gcgcgctcca ggtagaggct ctggttggcc cgcggtgtct cgccttcgac gatcagcacc
     4501 ggcacccgtt gttgatccga atagacgaaa tgggcgtggt tatcgatatc cagcgcatcg
     4561 ctgtcgaggc tggcgacgcc gcgcagcagg ccggctcccg gggtcagtcc gtcgaagcgc
     4621 acgaccctgg tctcgccggc ggccagttgc aggattcgcg gcggcaactg gcggccatcg
     4681 aggctcagca ccaggcgttg ctcgcgctcg cgctcgccgt ggttgacgat ttccaccgcc
     4741 aggccgaact cgtccacggc gccgtccgcc ggcgcctcga tgacgagcgc cgcgatcgcg
     4801 gcattgtcgg cggccgcggc attcaccgcc agggcctcga cctcgatgcc gtcgtcgata
     4861 cgcggaattt ctccggccgc ggcctggaaa tccgagatca gcaggattcg gcggcgcacc
     4921 gcgttgctgc cctccagcag gcgagcggcc tgctcgaccg ccgcccgcag gcgcgtgccg
     4981 cgcaggccgg gctgctggcg tcgaatggcg ccgcgcaggt cgtcggggtt atcggtcagg
     5041 tcgctggcca gctcggtgtc ggcatcgaag gcgatcacgc cgagccgatc cccgggacgt
     5101 tttgtgtcga ccagcccgag ggcgagttgg cgtgcctcgt cccagcgctc gccgatgcgc
     5161 atgctatagg agcgatcgat gacgatgacg ccgtcgctgc gctcggtatt cggcattacc
     5221 gcggcgccgc tgtcgaagaa cggccgcgca aaggccagtg cgagcaatgc cagcagcagg
     5281 cagcgcagca gcaacagcag ccagtggcgg atttcgagac ggcgtttctc gcgccacggg
     5341 atttgccgca ggaacatcag cgacggaaac ttgaggccct gggagtgccg ccgctggacc
     5401 aggtgaatca acaccggcag cgccagcagc agcagtccgg cgaggtacag cgggttgatc
     5461 cacgccagtg ccatcagcgg gtccgcttgc gccgttcgcg gtccgacagg tagcggtaga
     5521 gcacgccgtc cagcggttgc gagatatcgg taaagatgta atcgatctgg ttgccgccca
     5581 gccggcgctc gagttcggcg cggtgctcat tcaccagctg ttggtaacgg tcgcgaaact
     5641 cgcgggtcga gatcggcagg tcgaccccgg tctccagttc ctcgacgctg gagacgtcgc
     5701 cgagctcgag gttcagttcc tgcgggtcga gcaggtgaaa cacgatcagg tcgttgccgc
     5761 gataacgcag ctggttgatc gccggcagca ggctttcggg cggctcgtag agatccgata
     5821 tcagcaccac caggctgcgc cgtcgcaggc tttctgtcat ccgctgcaat ggccggtggt
     5881 aattgctgac gccctcggcc tggacgcggt cgagcgcatg cagcaccagg ttgaaatgcc
     5941 gaatcgacgg cggaatggtg tcgacgatat cgtcgctgaa ggtgaccagg ccgatacggt
     6001 cgcgctggcg cgtcgacagg taggcgaggc tcgcggccag gtacttggcg tactccagct
     6061 tggtcacgcc gccgctgccg aagtccatcg atttcgatac gtccagcagc aggtgcaggt
     6121 tggcgttggt atcggcttcg aattccttca cgtacagccg gtcggtgcga gcgaacaggc
     6181 gccagtcgat gcgccgcacg tcatcgcccg gcatgtagac ccggtgctcg gcgaaatcca
     6241 ccgacatgcc gagcatcggc gactggtgca gaccgctgat gaagccgtcc accacgcggc
     6301 gcgcgatcag ctcgaggttg tcgatgcgcg tcagcaccgc cgggtcgatg aagcgctgtc
     6361 ccggcagctg tgcctgcgtg gtcttcgcac ccagcatggc gaatcccgct acattcccga
     6421 ttccggcacc ggcaccgctt ccagcaggcg gtcgatgatt tcatcgctgc cgattttttc
     6481 ggattcggcg tggaaattgc gcagcacgcg atggcgcagc acgggctttg ccaacgccgc
     6541 gatatcgtcg aagctgacgt ggtagcgacc gtcgatcagc gcccgagttt tgccgcccag
     6601 taccaggttc tgggcggcgc gcgtgctggc gccgtaggca acccagtcgt tgacgaaagc
     6661 gggggccgca tcgctgcccg ggcggctgca gcgcaccagg tcgagcgcat attgcgatac
     6721 cgcccgcgac accggcacgc ggcggacgag tttctgaaag gcgcgaatct gttcgccgtt
     6781 catcgcgtac tcgaatttga tgtcgcgtag cgaagtggtc tggcgaacca cttcgagttc
     6841 atccgcggcc gacaggtaat cgatgacgat ctcgaacatg aagcggtcct gttgggcctc
     6901 gggcagcggg taggtgccct cgagttcgat cgggttctgg gtcgcgaaga cgaaaaaggg
     6961 ttcctcgaga tcgtaggtct tgccctgcac ggtgacgcgg tgttcctgca tcgcctcgag
     7021 tagcgccgac tgcgtcttcg gcggcgtgcg attgatctcg tcggcgagca cgatgttggc
     7081 gaagatcggc ccgggcagga aaaccagctc gcgcttgccg ctgtcgggat tttcctgcac
     7141 caggtcggtg ccggtaatat ccgacggcat caggtcgggg gtgaactgaa tacgcgagaa
     7201 tttcaggtcc agcacctgcg cgatcgagtg aatcagcagc gtcttggcga gaccggggac
     7261 gccgacgatc aggctgttgc cgccggccag tatggtgatc aatacctgct cgacgacgct
     7321 gtgctggccg acgatcagtt tggccagttc ctctcgcgcc cggccgtagc gtgcgcgcag
     7381 gtcctcggcg agacggatgt cgtcttcccc ttgcagggtt gtcgcctgtt ctgtttcgat
     7441 atccacggac ggtttcctcg tagcggttgc gatgcagcgc ggtgagcgcg cgctatggtg
     7501 gcggctgcac ggttgcgccg acctggtgca gggccagcag caactcctgt gcctggaaat
     7561 aattgggcgc gatctccagt gcgtatagta tctgctcgcg tgccgaagat cgattgccgg
     7621 cacgttggta ggcgcgcgcc agctggtaat gcgcctcggc gcgatcgatc ggatcgagtg
     7681 ccagtatcgc gcgtcgctcg cgagccacgg cctgccagcg ctcgagtttc tccaggtgac
     7741 cggcgagtgc gtggtgcagg tccgggtcgt agggataaat atagatcgcc gactgcagcg
     7801 cctcggcggc gccggcgtca tcgccgcgcg cggcgcgcag gctcgccagc tgccgttgtg
     7861 ccgcgtagtg ctcggcattg atcgcggtca tgcgcgccag ctgctcctcg gccgcgtcgg
     7921 gccggttctg ctgctggtag agcgacgcca gcagccagta gctgctgtca ccgccggcat
     7981 attgcggaaa cagttgctgc gcccgcgtca ggaatagctc ggccagttcg aggtcgccct
     8041 tgtcgtacag cgagatgccc agccgcagct ggtcgaaata ccggtcgggc tgcgcgttgt
     8101 cgatcgacgg gccgctttcc gtttcaccgg ggtcgagctc gtcgacggtg ccctgcaggg
     8161 ccgccagcgg aaccgcgtag cgctttctga aatactcgtc aaaggcggcg tcgagcgccg
     8221 ccgcatcgag ctcgagcacg gccggtagca ccgcgtcggg gtcgctgctg tcgcggtagg
     8281 cgttgagcgc ttcgcggatg gcgtcgaaac cccggcggga ctcgatgaaa tcgaacacca
     8341 gcgatgcctg gaaataggaa tggacgacct gttccgggta actcgggcgc acgaagccgc
     8401 tgttgagctg cgacagcggc agcaattgcc cggcgatgta ggcgcgcaga aagccgggat
     8461 cgacgtcacc gccccagccg ggacgcccct ggcgctcctc gtagaccgcg agcccctcgg
     8521 agaaccagcg cggcacgcga ttgtcggtca tcgtcaggtg aaacacgtgc gccagctcgt
     8581 gccacagcgt cgatccccag ttgaacgcgc cacgctcgcg tgcggccggc gaatccatcg
     8641 ccaccaccgg accgaaggcg acaccgagca ggccgacgcc ggcgaggccc accgtgcgta
     8701 ccgagaagtc ggcgtgattg gggtagaatt cgacgcgaat cggcgcctgc gggcggtagt
     8761 cgtagcgctc ggcgaaggct tcgtaagcct gctcggcgat ctcgtgcacg tattcgccga
     8821 gcaaagcctg ctcgttgtcg tgcaatacca cgcggaactt cccgcgatcg gtttcggcgt
     8881 agttttcgaa gctgtccgcg agctgcaggg tattcttgat ccagacgttg tagggatccc
     8941 ccgcaaacga gcgttcgagg ttctccttgc cctcgaccat tgcgccgcgg cgc
//
LOCUS       DATCZV010000291         4979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_658802, whole genome shotgun sequence.
ACCESSION   DATCZV010000291 DATCZV010000000
VERSION     DATCZV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658802"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIW27_06460"
     CDS             complement(<1..410)
                     /locus_tag="VIW27_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY5739350.1"
                     /translation="MDALQWNIATVASVIDIAVAPVFLLAGISGLLVVLTNRLGRTID
                     RSRSLQATESGFLPQAHREAIDRELDALLMRIRLAYTAISLSTLSAILVCLVIVALFL
                     GTLMQFNVSLFVASLFIFCMIILSLAFAAFLLEVL"
     gene            complement(419..1432)
                     /locus_tag="VIW27_06465"
     CDS             complement(419..1432)
                     /locus_tag="VIW27_06465"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEY5739351.1"
                     /translation="MSLTRGFPRASLSHLPTPLERLANVSAEFGSNLWIKRDDCTGLA
                     FGGNKSRQLEFYIGQALEQGADTLLTTGAVQSNHVRMTVAAARKLGLDVEVQLEHRVE
                     REQPEYHNSGNPYLVKLMGAKIHHYPVGEDEHGADRALEERAIELAAEGRKAYVIPLS
                     NAHAPYGALGYVDAAEELLQQLNDAQVDATRFIVPSGSASTHSGFLVGLRARGCATPV
                     QGVCVRRDAESQKQRVIAKMRAVIDTLGVDLEIDDDDVLCDDRMLAPGYGLPSAATVE
                     AIEYLARREGILLDPTYTGKTFAWLLASLRNGDYGRGDHLVFLHTGGAPSLFGYPELV
                     SDA"
     gene            complement(1447..2292)
                     /locus_tag="VIW27_06470"
     CDS             complement(1447..2292)
                     /locus_tag="VIW27_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739352.1"
                     /translation="MTPSIVVLVLLAALLHAAWNAMAKSGGAPVYSIASYRLTSALCC
                     LPLVLLLPLPQAASWPMLIASILIHTAYYVTLARSYHSGDLSQVYPLFRGLAPVLVAL
                     GAAGLAGEYLGGGALLGILLVSAGLVSISFAGGALGRISSPALGWGLATSVLIAAYTI
                     ADGMGVRVAGNAFSYIAWLFLLEPVPICGWLLLRQRAGWFGYMRSRPGKIAAGAFAAA
                     AAYGMVIYAMGVAPMGLVSSLRETSVIFAAIIGTLLFREPFGRQRIVAALLVCSGVVL
                     IKILS"
     gene            complement(2374..4977)
                     /locus_tag="VIW27_06475"
     CDS             complement(2374..4977)
                     /locus_tag="VIW27_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020286942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEY5739353.1"
                     /translation="MVAQVDTGGRAPTGMVAQFIAWTAFTWAVFQLYIASNLPFWLSD
                     VTGLGFWVVTNSDARLYHLAFGLFLAALAFPLLKSSPRDRVPWYDWALGIIGVACCLY
                     IVVLRNEIAVRAGLPTQGDLIASTIGMIILGVTVYRALGLPLLIVASVFVAYVFFGNS
                     PSLPEAIQWKGASYGKAMWHFWMQNEGVFGVALGVSASLIFLFVLFGSILEKAGAGNY
                     FIKIAFALLGHLRGGPAKAAVVASALSGLYSGSSIANVVTTGTFTIPLMKRTGFSPEK
                     AGAVEVASSTNGQLTPPVMGAAAFLIAEFTGISYTDILKHALVPALVSYIALVYIVHL
                     EAMKMNLKGLPKPPSNKTFAMKMIGFLSGFIGIGVLGMIVYFGLGWIKQVIPDYAFFI
                     VSVGCAAAYLYLVWVASKRPDLVVDPPDAPITELPRAGDVAVTGLYFLLPIVILIWSI
                     VVERLSPALSAFWATIAMIIVILTQDALKSFFRGTGNYGAAIRTGLGHWVEGMIAGSR
                     NMISIAVATGAAGIIVGTISLTGAHQVIGEFVEFLSGGSLIFMLLLVAVMSLILGMGL
                     PTTANYIVVSSLMAPVIITLGAQNGLIVPLIAVHLFVFYFGILADDTPPVGLAAFAAA
                     AISGGDPIKTGIQGFAYDIRTALLPFLFIFNTELLLIDVSVAKAFFVFGVAVIAMMLF
                     ASATQGFWFVRNKVWESGLLLLVAFMLFRPGYFLDRFVPPFDVFEPDRVFEVVDGVPA
                     GGEMIAVVSGPDFDTGEIMSTTIVVPLAAGADGESRLGAAGLSVTIDGGKAVIEEPFP
                     GTPYFESLGKAFDYYGDEPVQVAEVRKEADRIFKEVVYIPAILLLALVFFLQKRRQRE
                     QQGSGAAEAPA"
BASE COUNT         1058 a         1601 c         1567 g          753 t
ORIGIN      
        1 agtacttcga gcagaaaggc ggcaaacgcg agactcagaa tgatcatgca aaaaatgaac
       61 aggctggcga cgaacagcga tacgttgaac tgcatcagcg tgccaagaaa cagtgccacg
      121 attaccaggc agacgaggat cgcactcaga gtcgacaggc tgatcgcggt atacgcgagg
      181 cgaatacgca tcagcagcgc gtcgagttcg cgatcgatcg cctcgcgatg cgcctgtggc
      241 aggaaccccg attccgtagc ctgcagcgag cgcgagcgat cgatggtgcg cccgagacga
      301 ttggtcagca ctaccagcag gccggaaata cccgccagca ggaataccgg cgccaccgcg
      361 atatcgatga cgctggcgac ggtggcgata ttccattgca atgcatccat cggcggactc
      421 acgcgtcact gaccagttcg gggtaaccga acagcgacgg tgcgccgccg gtgtgcagga
      481 ataccagatg atcgccgcgc ccgtaatcgc cgttgcgcag gctggcgagc agccaggcaa
      541 aggttttgcc ggtgtaggtc ggatccagca gtatgccctc ccgcctggcg agatactcga
      601 tggcttcgac ggtggccgcg ctcggtaacc catatccggg cgccagcatg cggtcatcgc
      661 agagtacgtc gtcgtcgtcg atctcgaggt cgacgccgag cgtatcgatg accgcgcgca
      721 tcttggcaat cacacgctgc ttctggctct ccgcatcgcg gcgcacgcag acgccctgca
      781 ccggcgtcgc gcaacccctg gcacgcagac cgaccaggaa gccgctgtgc gtcgacgccg
      841 atcccgacgg cacgatgaaa cgcgtcgcgt cgacctgtgc gtcgttcagc tgctgcagca
      901 attcctcggc ggcatcgaca tagccgagcg caccgtaggg cgcatgcgca ttggacagcg
      961 gaatcacgta ggccttgcgg ccctcggcgg cgagttcgat ggcgcgctcc tcgagggccc
     1021 ggtccgcgcc atgttcgtct tccccgaccg ggtaatggtg aatcttcgca cccattagtt
     1081 tcaccaggta gggattgccc gagttgtggt attccggttg ctcgcgctcg acgcggtgct
     1141 cgagctgcac ttcgacatcg agccccagct tgcgcgccgc ggccacggtc atgcgcacgt
     1201 ggttggattg caccgcgccg gtggtcaaca gcgtatcggc gccctgctcg agtgcctggc
     1261 cgatataaaa ttcgagctgg cggctcttgt tgccgccgaa tgccagcccg gtacagtcgt
     1321 cgcgctttat ccacaggttg ctgccgaact cggcactgac gtttgccagc cgctccagcg
     1381 gtgtcggcag gtgactcagg ctggcgcggg ggaaacccct ggtcaaagac atttcattcg
     1441 ctccgttcag cttagaattt taatcaatac cacgccgctg caaaccagca gtgcggcgac
     1501 gatgcgctgc ctgccgaacg gctcgcgaaa cagcagggtg ccgatgatcg cggcaaatat
     1561 tacactggtt tcgcgcagtg acgaaaccag ccccatcggt gcgactccca tcgcgtagat
     1621 caccattccg taggctgccg ccgcggcgaa cgcgccagcc gcgatcttgc ccgggcgcga
     1681 gcgcatgtag ccgaaccagc cggcgcgctg gcgcagcagc agccagccgc agatcggcac
     1741 gggctcgagc aggaacagcc aggcgatgta actgaatgca ttacccgcca ccctgacgcc
     1801 catgccgtcg gcgatcgtgt aagccgcgat cagcaccgag gtcgcgagac cccagccgag
     1861 cgccggcgac gatatacggc cgagtgcgcc acctgcgaaa ctgatactga ccagaccggc
     1921 gctgaccagc aggatgccga gcagcgcgcc gccgccgagg tactcgccgg caagcccggc
     1981 cgcgccgagc gcgaccagca ccggggcgag gccgcgaaac agcggataga cctgcgacag
     2041 gtcaccgctg tggtacgacc gcgccagggt tacatagtag gcggtgtgaa tcaggatcga
     2101 cgcgatcagc atcggccagc tggcagcctg cggcagcggc agcagcaaca ccagcggcag
     2161 gcagcagaga gcgctcgtca ggcgatacga agcgatgctg tagacgggtg caccgccgga
     2221 cttggccatc gcgttccacg ccgcgtgcag caacgcggcc agcagcacca gcacaacgat
     2281 cgacggggtc acggcgcgtt actcacggcc aggcgcggca gccgtgctgc cagggacagc
     2341 caggccgctt acgctgaaca atgacgccgc ggctcaggcg ggcgcttcgg ccgcgccact
     2401 gccctgctgc tcacgctgac gccgcttctg caggaagaag acgagggcca gcagcaggat
     2461 cgccggaata taaacgacct ccttgaagat gcgatccgcc tccttgcgca cctcggccac
     2521 ctgcaccggt tcgtcgccat agtaatcgaa ggccttgccc agggactcga agtaaggcgt
     2581 gcccgggaag ggttcctcga tgaccgcctt gccgccgtcg atcgtcactg acaatccggc
     2641 cgcgccgagg cgcgattcgc catccgcacc cgcggccaga ggcacgacga tggtcgtcga
     2701 catgatctcg ccggtatcga aatccggacc gctgacgaca gcaatcattt cgcccccggc
     2761 cggcactcca tcgacgactt cgaacaccct gtccggctcg aacacgtcga acggcgggac
     2821 gaatctgtcc aggaaatagc cgggacggaa cagcatgaag gcgaccagca gcagcaaccc
     2881 ggattcccag accttgttgc gcacgaacca gaagccctgg gtcgccgacg caaacagcat
     2941 catcgcgatg accgcgacgc cgaagacgaa aaaggccttg gccaccgaga catcgatcag
     3001 cagcagctcg gtgttgaaaa tgaacaggaa cggcaacagc gcggtgcgga tatcgtaggc
     3061 gaacccctgg ataccggtct tgatcggatc accgccggaa atcgccgcgg cggcgaaggc
     3121 cgcaagcccc accggcggcg tgtcgtcggc gagtatgccg aagtagaaca cgaacaggtg
     3181 caccgcgatc aacggcacga tcaggccgtt ctgcgcgccg agtgtgatga tgaccggcgc
     3241 catcagcgac gaaaccacga tgtagttggc cgtggtcggc aggcccatgc cgaggatcag
     3301 gctcatcacc gcgaccagca gcagcatgaa gatcaggctg ccgcccgaca ggaactcgac
     3361 gaactcgccg atcacctggt gggcgccggt aagcgaaatg gtgccgacga tgatgcccgc
     3421 ggcaccggtg gcgaccgcga tgctgatcat gttgcgcgaa ccggcgatca taccctcgac
     3481 ccagtggccg aggccggtcc tgatggcggc gccgtagtta ccggtgccgc ggaagaatga
     3541 tttcagcgca tcctgggtca ggatcacgat gatcatcgcg atcgtcgccc agaacgcgga
     3601 cagcgctggt gacaggcgct cgacgacgat cgaccagatc aggatcacga ttggcagcag
     3661 gaaatacagc ccggtgaccg caacatcgcc ggcgcgcggc agctcggtga tcggcgcgtc
     3721 cggcggatcg acgaccaggt ccggccgttt cgaggccacc cagaccaggt acaggtaggc
     3781 ggccgcacaa cccacggaaa cgatgaagaa ggcgtaatcc ggaatcacct gcttgatcca
     3841 gccgaggccg aagtagacga tcatgccgag cacgccgata ccgatgaagc cgctgagaaa
     3901 gccgatcatc ttcatcgcga aagtcttgtt cgacggcggc ttgggcaagc ccttgaggtt
     3961 catcttcatc gcctcgagat gcacgatgta caccagtgcg atataggaca ccagcgccgg
     4021 caccagtgcg tgcttgagga tatcggtata gctgatgccg gtgaattcgg caatcaggaa
     4081 ggccgcggcg cccattaccg gcggcgtcaa ctgaccgttg gtcgacgacg cgacctcgac
     4141 cgcgccggct ttctccggcg agaagccggt gcgcttcatc agcgggatgg taaaagtacc
     4201 ggtggtgacc acgttcgcga tcgaggatcc cgagtacagg ccgctcagcg cggaggcaac
     4261 caccgccgcc ttggccggcc cgccgcgcag atggccgagc aacgcaaacg cgatcttgat
     4321 gaaatagttg ccggcgccgg ccttttccag aatcgagccg aacagtacga acaggaagat
     4381 caacgatgcg gacacgccga gcgcgacgcc gaatacgccc tcgttctgca tccagaaatg
     4441 ccacatcgcc ttgccgtagg acgcaccttt ccactggatc gcctcgggca ggctcggcga
     4501 gttaccgaaa aacacgtagg ccacgaatac gctggcaacg atcagcagtg gcaggccgag
     4561 cgcgcggtag acggtcacgc cgaggatgat catgccgatc gtcgacgcga tcaggtcgcc
     4621 ctgggtcggc aacccggcgc gtaccgcgat ctcgttgcgc aacacaacga tatacaggca
     4681 gcaggcaacg ccgatgatcc ccagtgccca gtcgtaccag ggtactcggt ccctgggact
     4741 ggacttgagc agcggaaacg ccagtgcggc gagaaacagg ccgaaggcca ggtgatacag
     4801 acgggcgtcg gaattggtga ctacccagaa accgagtccg gtgacgtccg atagccagaa
     4861 cggcaggttc gatgcgatat agagttggaa caccgcccag gtaaacgcgg tccaggcgat
     4921 gaactgcgcg accatgccgg tcggcgcccg cccaccggta tcgacctgcg caaccaggt
//
LOCUS       DATCZV010000292         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_393905, whole genome shotgun sequence.
ACCESSION   DATCZV010000292 DATCZV010000000
VERSION     DATCZV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393905"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="amrS"
                     /locus_tag="VIW27_06480"
     CDS             complement(<1..474)
                     /gene="amrS"
                     /locus_tag="VIW27_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012323697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY5739354.1"
                     /translation="MNTIVSTRYWHRLDDGRLQCDLCPRHCKLREGQRGLCYVRQRLD
                     DEVKLVSYGRSSGFCIDPIEKKPLNHFYPGSAVLSFGTAGCNLACKFCQNWDMSKSRE
                     MDTLADAASPAKLSRVAAEMSCRSIAFTYNDPVIFHEYALDVAAACRERNIRTVAV"
     gene            556..1338
                     /gene="amrB"
                     /locus_tag="VIW27_06485"
     CDS             556..1338
                     /gene="amrB"
                     /locus_tag="VIW27_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY5739355.1"
                     /translation="MTTRAAAVAGYFYDADPLRLQHHVSQLLNAAPADSESLPRALIV
                     PHAGFVYSGSTAAFAYKCLALDRDQVQRVLLIGPAHRVYVSGMAIPSVDRFATPLGAI
                     PLDREGLDAIADLPGVEVSDEAHRDEHSLEVQLPFLQSVLGDFSLVPVVVGGAVPAQV
                     AAVIDALADDPATLTVISSDLSHFRRYDEARRIDSATCEEILARATTLNGEQACGARA
                     INGLMASRAARDLKISLLHACNSGDTAGSRERVVGYAAFALV"
     gene            1316..1894
                     /gene="amrA"
                     /locus_tag="VIW27_06490"
     CDS             1316..1894
                     /gene="amrA"
                     /locus_tag="VIW27_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY5739356.1"
                     /translation="MQPSLSFEIDSAAESRLLEIARESIEAGAAAERAPRIDVDGCAG
                     LLQPAAVFTTLTRDGALRGCVGSLQAQRPLAQAVADSAFNAAFHDRRFKPLDVGEFGL
                     VRIEISVLSAMQPIDAPTRQALLEQLQPGIDGLLVEDRGCRATFLPKVWENLAAPGAF
                     VDQLLVKAGLGAGYWSQTLSLYRYHTHSFGEK"
     gene            1986..>2564
                     /locus_tag="VIW27_06495"
     CDS             1986..>2564
                     /locus_tag="VIW27_06495"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF037184.4"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY5739357.1"
                     /translation="MLCLPGSPALSQFRLEKLKRQLGEAGYRIDGLGSRYIHLVDSAA
                     PVDEKAREILDNLLTYGPAMPPESVDGEQYYVVPRPGTISPWASKATDIAHNCGLAQV
                     RRIERGIVYSIDGGGELPLELLHDRMIEAVFTSLEQCAALFASHQPRPLVEVDTTRHG
                     RAALEQANSDLGLALSDDEIEYLAAAYAQLGRN"
BASE COUNT          444 a          829 c          816 g          475 t
ORIGIN      
        1 aaccgcgacc gtgcgaatat tccgctcgcg gcaggccgcc gcgacatcta gcgcatactc
       61 gtgaaagatc accgggtcgt tgtaagtgaa ggcgatactg cggcaggaca tctcggccgc
      121 tacccgggaa agctttgccg gcgatgcggc atccgccagg gtatccattt cgcgcgactt
      181 gctcatatcc cagttctggc agaatttgca cgccaggttg cagcccgcgg tgccgaacga
      241 cagcaccgcg ctgccggggt aaaaatggtt gagcggtttt ttttcgatcg ggtcgatgca
      301 aaatccgctg gaacggccgt aactgaccag cttgacttca tcgtcgagcc gctggcgcac
      361 gtagcagaga ccgcgttgcc cctcgcgcag cttgcagtgc cgcgggcaca ggtcgcattg
      421 cagccggccg tcgtcgaggc ggtgccagta gcgggtggag acgatcgtat tcatgcttca
      481 accgacctgg atcaatcgat cgccgccggg ttcggctata atcgtgtctg tgatgcaaac
      541 cggtagaggc aaacaatgac cacgcgtgcc gcggccgtag ccggttattt ctacgatgct
      601 gatccgcttc gcctgcaaca tcatgtcagc cagttgttga acgctgctcc cgccgattcg
      661 gaatccttgc ccagggcact aatcgtacca catgcgggct tcgtctactc cggatcgacc
      721 gcggcattcg cctataagtg ccttgcgctt gaccgggatc aggtgcagcg ggtgctgctg
      781 atcggcccgg cgcaccgggt ttacgtcagc ggcatggcga ttccgtcggt ggatcgcttc
      841 gcgacaccgc tcggtgcaat cccgctcgac cgtgaaggcc tcgacgcgat cgccgatctg
      901 cccggggtcg aagtatccga cgaggctcac cgcgacgagc acagtctcga ggtgcagctg
      961 ccattcctgc aatcggtcct tggcgatttc agcctggtgc cggtggtggt cggcggtgcc
     1021 gtccccgcgc aggtggcggc ggtcatcgat gcgctggcgg atgacccggc aacgctgacc
     1081 gtgatcagca gcgacctgtc gcatttcagg cgctacgacg aggcacggcg tatcgattcg
     1141 gcgacctgcg aggaaatcct cgcgcgcgcc accacgctga atggcgaaca ggcctgcggc
     1201 gcgcgcgcga tcaatggcct gatggcctcg cgtgccgcgc gcgaccttaa gatcagtctg
     1261 ttgcacgcct gcaattccgg ggataccgcg ggttcccgcg aacgagtagt cggttatgca
     1321 gccttcgctc tcgtttgaaa tcgacagcgc ggcggagtcc cgcttgctgg agatcgcgcg
     1381 cgagtcgatc gaagcgggtg ccgctgccga gcgagcgccg cgcatcgatg tcgatggttg
     1441 cgcgggcctg ctgcaaccgg cggcggtatt taccactctg acccgcgacg gtgcactgcg
     1501 cggttgcgtc ggctcgctgc aggcgcagcg gccgctcgcc caggcggtcg ccgattcggc
     1561 cttcaacgcg gcgttccacg accgccgttt caagcccctc gatgtcggcg agttcgggct
     1621 ggtgcgtatc gagatttcgg tgctgtcggc gatgcagccg atcgatgcgc cgacgcgcca
     1681 ggccctgctc gagcagctac agccgggcat cgacggcctg ctcgtcgagg accgcggctg
     1741 ccgtgcaacg ttcctgccca aggtgtggga aaacctggcc gcgcccggcg cgttcgtcga
     1801 ccagttgctc gtcaaggccg ggctcggcgc cggttactgg tcgcagaccc tgagcctgta
     1861 ccgctaccac acccacagct tcggcgaaaa atagcgcggc ggcgcgcgat cgtgttggag
     1921 gcgcagggtc ttttcggcta tcatttgcgc tctttttcga tcctcatcac gcgacccgaa
     1981 ccaatatgct ttgcctgccc ggtagcccgg cattatccca gttcagactg gaaaaactga
     2041 aacggcaact gggcgaggcg ggataccgaa tcgacggact cggcagccgc tatatccatc
     2101 tggtcgacag cgccgccccc gtcgatgaaa aggcgcgcga aatcctcgac aacctgctga
     2161 cctacggccc ggcaatgccg ccggaaagcg tcgatggtga gcagtattac gtcgtgccgc
     2221 gccccggcac catctcgccg tgggccagca aggctaccga tatcgcccac aactgcggcc
     2281 tcgcgcaagt gcggcgaatc gagcgcggca tcgtctacag catcgacggc ggcggtgagt
     2341 tacccctcga actgctgcac gaccgcatga tcgaagccgt gtttacctct ctcgagcaat
     2401 gcgcggcgct gttcgccagt caccagccgc ggccgctggt cgaagtcgat acgacgaggc
     2461 acggccgtgc ggcgctggag caggccaata gcgatctcgg cctggcactg tccgatgacg
     2521 agatcgaata cctggcggcg gcctacgcgc agctcggccg caat
//
LOCUS       DATCZV010000293         5595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_341426, whole genome shotgun sequence.
ACCESSION   DATCZV010000293 DATCZV010000000
VERSION     DATCZV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341426"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VIW27_06500"
     CDS             complement(<1..182)
                     /locus_tag="VIW27_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HEY5739358.1"
                     /translation="MTQLTNIVRLSFRDYSHEWRMSGCFVLALASVLAPMMILFGLKF
                     GIVSSMVQELVENPDNR"
     gene            complement(182..907)
                     /locus_tag="VIW27_06505"
     CDS             complement(182..907)
                     /locus_tag="VIW27_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739359.1"
                     /translation="MTVIYSLSNVVKERLNHGVGFRLVIPQIQISARENIAVIGHSGC
                     GKSTLLDMLALILRPDHSDEFSLHPTDGDNHDVASLWQRDRQGRLSQIRKQHIGYVLQ
                     TGGLLPYLTVRENIELPRKLLKLPDDDSISSLSRVLGIDRQLDKLPGLLSAGERQRAA
                     FARALSHRPTILIADEPTSALDPITAQKIMAVVMEVIRGLKITLITASHDWAHVYKME
                     LRTLKQEAEAVDGGKVYQSRFTD"
     gene            complement(915..2975)
                     /locus_tag="VIW27_06510"
     CDS             complement(915..2975)
                     /locus_tag="VIW27_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY5739360.1"
                     /translation="MMRSFRNSLAGLLLAALCALPTGAFGALDKPLLMEGKKSLYQRV
                     LSVPAARLYAQPDDSSAQEEIVPFSVLYVYQRDGDWLRVGHDSFGQIAGWVRRERAIA
                     WNQALTVSFKDPQNIQRVMLFNSKQSLEKLVDDYDRDAYQQLYEAAVANRAPPDSPVI
                     AIQPEAHLEIRENFYLVPIKQHEDVYLGNEQARLLEIASVPLDDRVPLDDRVPLDDSG
                     GEEPQQAIEESTRSYRSGIHFVIDSTQSMGPYIDRTREAVAKVYSAIESQGLTNQVSF
                     GLTAYRDNLAQVPELEYLTRRYVSMEQGTDVDQFFERVNSLSPSPVSSRDFREDAYAG
                     IKSAIEDSNWEQFDARYVVLITDAGPRESHDSLGATRLSAQALRQLAYDKGVAIWVLH
                     LRTPVAAADHQQAETLYKQLSFYPGIGDFYYGVSLGQVDEFGTVLEVLAGQITQQVLA
                     TTNGLPPLPLPEQERQPVQQAQQVQQAPQEPQEPQEPQQPSQLEQLQGRVAKLGNALR
                     MRYIQKESGQPLPAVFDAWMVDRDFMNPERSAVDVRVLLTRDQLSDLKNVMQQVLELA
                     EEGVLSPQNFLDDLKSLAATVSRDPSSVAGSTSGAGANLADMGYMREYIEDLPYTGEV
                     MNLTLESWQEWSAKMQIEFMHRLESKINYYQALHDHTDLWVTPGGGAVNGNSVFPVAL
                     ELLP"
     gene            complement(2984..3556)
                     /locus_tag="VIW27_06515"
     CDS             complement(2984..3556)
                     /locus_tag="VIW27_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739361.1"
                     /translation="MPDQDEPESPPRRNPLLWIALVVAGLIVYVVTSTDRGGKLPVEN
                     GSASIVTSGSVERTLLVSPGMRAREYIAGLRAAGEPYPLAEAHQKAGEFLREGNLADA
                     HLLYFFSAREDHLPSIMTMGEMSDPLHFSAENALLDHADAVQAYKWYSKAASLGQQTA
                     EERIGDLRQWASVEAANGNPEARQLLLNFQ"
     gene            complement(3799..4341)
                     /locus_tag="VIW27_06520"
     CDS             complement(3799..4341)
                     /locus_tag="VIW27_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739362.1"
                     /translation="MASWRGVPAMCCGVCAASLARRLALLLLLSAVLQGCGFRLAGTA
                     TLPPELARIQLETRDFDNRQRDVLLRRLQRAGAEVSVEPAADRTRLSVRLLAPRDRRL
                     VTSASSGRNVNRVSRVLEYSLWSADGQLRAGPSSLTAGTDVTLDDDSLLSSSDERQSA
                     IEELETNLYDQLIRQLQRLQ"
     gene            complement(4334..>5595)
                     /locus_tag="VIW27_06525"
     CDS             complement(4334..>5595)
                     /locus_tag="VIW27_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5739363.1"
                     /translation="LESAGKGTRQINYRLRDWGVSRQRYWGAPIPVINCSACGQLPVP
                     ENDLPVVLPEDVEFEGIGSPIKKMESFIRTTCPQCGGPAERETDTFDTFMESSWYQER
                     FTCPDQQQAMLDERTAYWAPVDLYIGGIEHAILHLLYARFYHKLMRDEGLLESGEPFK
                     RLLTQGMVLKDGSKMSKSKGNTIDPQELIDRYGADTVRLFIMFASPPEQSLDWSDSGV
                     EGAYRFLKRFWAAVQEHLAEAHEVTAVDAAALDDAQKAMRLKLHETIAKVSDDFERRL
                     TFNTAIAANMELLNSVSRFDDDSAAGTAIRREVFTSMVLMLAPIIPHICHRLWQDLGH
                     EEPLIDHPWPEVDDAALVQDSIEMVIQVNGKLRGRMQVAADADREACEAAALANEQVT
                     RFIGDGVVRKVIVVPGKLVNIVVQANG"
BASE COUNT          944 a         1805 c         1779 g         1067 t
ORIGIN      
        1 cgattgtccg gattttccac cagttcctgc accatgctgc tgacgatacc gaacttgaga
       61 ccgaacagaa tcatcatcgg cgccagcacc gacgccagtg caagcacgaa gcagcccgac
      121 atgcgccact cgtggctgta gtcgcgaaag ctcaggcgaa cgatattggt gagctgcgtc
      181 atcaatcggt aaatctcgac tggtacacct tgccaccgtc gaccgcctcg gcttcctgtt
      241 tgagggtgcg caattccatc ttgtagacgt gcgcccagtc gtgactggca gtgatcagcg
      301 tgattttcag gccgcggatg acttccatca ccacggccat gatcttttgc gcggtgatcg
      361 gatcgagcgc cgaggtcggt tcgtcggcga tcaggatcgt cggccggtgc gacagcgcgc
      421 gcgcaaacgc ggctcgctgg cgttcgccgg cggacagcag gccgggcagc ttgtcgagct
      481 ggcggtcgat acccagcacc cgcgacagcg aactgatcga atcgtcgtcc ggcagtttca
      541 gcaacttgcg cggcagctcg atgttttcgc gtaccgtcag gtacggcaac aggccgccgg
      601 tctgcagcac gtaaccgata tgctgcttgc gaatctgcga cagcctgccc tgccggtcgc
      661 gctgccacag gctggcgaca tcgtgattgt cgccgtcggt cgggtgcagg ctgaactcgt
      721 cgctatggtc cgggcgcagg atcagcgcca gcatgtcgag caaggtgctc ttgccgcagc
      781 cgctgtggcc gatcaccgcg atattctcgc gcgcactgat ctggatctgg ggaatcacca
      841 ggcgaaaacc gacgccgtgg ttgagccgct ccttgacgac gttgctgagc gaatagatga
      901 cggtcacagg ctgtctacgg cagtaattcg agggcgaccg ggaataccga gttgccattg
      961 accgctccgc cgccgggggt tacccagaga tcggtatggt cgtgcagggc ctggtaataa
     1021 ttgatcttgc tctccaggcg atgcatgaac tcgatctgca tcttggccga ccattcctgc
     1081 cagctctcca gcgtcaggtt catgacttcc ccggtatacg gcaggtcctc gatgtattcg
     1141 cgcatatacc ccatgtcggc caggttggcg ccggcgcccg aagtgctgcc ggcgaccgag
     1201 gacgggtcgc ggctgaccgt ggccgccagg ctcttcaggt cgtcgaggaa attctgcggg
     1261 ctcagcacgc cttcctcggc cagttccagc acctgctgca tgacgttttt cagatcgctc
     1321 aactggtcgc gggtcaacag cacgcgcacg tcgacggcgg agcgttccgg gttcatgaag
     1381 tcgcgatcca ccatccacgc gtcgaacacc gcaggcagcg gctggccgga ttccttctgg
     1441 atatagcgca tgcgcagcgc gttgccgagc ttggcgacac ggccctgcaa ttgctcgagc
     1501 tggcttggct gctgcggttc ctgcggttcc tgcggttcct gcggcgcctg ttgcacctgt
     1561 tgcgcctgtt gcaccggttg gcgttcctgc tccggcaacg gcaacggcgg caggccgttg
     1621 gtggtcgcca gcacctgctg ggtgatctgc ccggcgagta cctcgagcac ggtgccgaat
     1681 tcgtcgacct ggccgagact gacgccgtaa tagaaatcac cgattccggg atagaaagag
     1741 agttgcttgt agagcgtctc ggcctgctgg tgatcggcgg ccgccaccgg cgtgcgcaga
     1801 tgcagcaccc agatcgcgac gcccttgtcg taggccagct ggcgcagcgc ctgcgcgctc
     1861 aagcgcgtgg cgcccagcga atcgtggctt tcccgcggac cggcatcggt gatcagcacc
     1921 acgtagcggg catcgaactg ctcccagttc gaatcctcga tcgccgactt gataccggcg
     1981 taggcatcct cgcgaaaatc gcggctcgaa accggcgacg ggctgagact gttgacgcgc
     2041 tcgaagaact ggtcgacgtc ggtgccctgc tccatgctga cgtaacggcg cgtcaggtac
     2101 tcgagctccg gcacctgggc caggttatcg cgatacgcgg tcaagccgaa gctgacctgg
     2161 ttggtgagcc cctggctttc gatcgccgag tagactttgg ccaccgcttc gcgcgtgcga
     2221 tcgatatagg ggcccatcga ctgcgtcgag tcgatgacga aatgaatgcc gctgcgatag
     2281 ctgcgcgtgc tttcctcgat cgcctgctgc ggctcctcgc caccgctgtc gtcgagcggc
     2341 acgcggtcgt cgagcggcac gcggtcgtcg agcggcacgc tggcgatttc cagcagccgc
     2401 gcctgctcgt tgccgaggta gacgtcctcg tgctgcttga tcggcaccag gtaaaaattc
     2461 tcgcgaattt cgaggtgcgc ctcgggctgg atcgcgatca ccggcgaatc cggcggtgcg
     2521 cggttggcga ccgcggcctc gtagagttgc tggtaggcat ccctgtcgta gtcgtcgacc
     2581 agtttttcca gcgactgctt gctgttgaac agcatcaccc gctggatgtt ctgcggatcc
     2641 ttgaaggaaa ccgtcagcgc ctggttccag gcgatcgctc gctcgcgcct gacccagccg
     2701 gcgatctgcc cgaagctgtc gtgaccgacg cgcaaccagt cgccgtcccg ctgataaacg
     2761 tagagcacgc tgaaggggac gatttcctcc tgtgccgacg agtcgtcggg ctgtgcgtag
     2821 agccgcgcgg cgggcacgct caacacgcgc tggtagaggc tcttcttgcc ctccatcaac
     2881 aacggcttgt cgagcgcacc gaaggcgccc gtcggcaacg cgcacagcgc cgcgagcagc
     2941 aggcccgcga gagaattgcg aaagcttcgc atcatcgatt cacctattgg aaattgagca
     3001 gtaactggcg cgcctccggg ttaccgttgg cggcctcgac gctggcccac tgccgcaggt
     3061 cgccaatccg ctcctcggcg gtctgctgac caagactcgc cgccttgctg taccacttgt
     3121 aggcctgcac cgcgtcggca tggtcgagca gtgcattctc ggcgctgaaa tggagcggat
     3181 cggacatttc gcccatcgtc atgatcgacg gcaggtgatc ttcgcgggca ctgaaaaaat
     3241 acagtaaatg ggcatcggcg agattgcctt cgcgcaggaa ttcgccggct ttttgatgcg
     3301 cttcagcgag tggatagggc tcgccggcgg cgcgcagccc ggcgatatac tcgcgcgcgc
     3361 gcattcccgg cgacaccagc agcgtgcgct cgacgctgcc gctggtgacg atgctcgcgg
     3421 agccgttttc aaccggcagc ttgccgccgc ggtcggtgct ggtgaccacg tagacaatga
     3481 gtccggctac gaccagcgcg atccacaaca acgggttgcg gcgcgggggt gattcgggtt
     3541 catcttgatc tggcatcggt ttttctagtg ctcggcaacg tcgaatcgag gttcgaagtc
     3601 gccctcaccg ttcgcgtttg gcagattata aacgcttgtc cgcggcgggt aaatttatcg
     3661 cggcccggta tcgtcgtcac cgagccgccg cagcccgaaa aacgcccact ccgttttcgc
     3721 gttcggccgc gtttatcggt cactcgagcg agcggcaact ggcggcagac ccgggccgcg
     3781 ggaatcgcgc gatcggcttt attgcaggcg ctgcagttgc ctgatcagct gatcgtagag
     3841 gttggtctcc agttcctcga tcgccgactg cctttcgtcg gacgacgaca gcaggctgtc
     3901 gtcgtcgagc gtcacgtccg tgccggcagt caggctacta gggccggcgc gtaactggcc
     3961 gtcggcgctc cacaggctgt attccagcac gcggctgacg cggttgacgt tcctgccact
     4021 gctggcgctg gtcaccagcc ggcggtcgcg cggtgccagc agccgtacgc tgagcctggt
     4081 gcggtcggcg gcgggctcga cgctgacctc ggcgcccgcg cgctgcagac ggcgcaacag
     4141 cacatcgcgc tggcgattgt cgaagtcgcg ggtctcgagc tgaatgcgcg ccagctccgg
     4201 cggcagagtc gcggtgccgg cgagccggaa tccgcaaccc tgaagtaccg cggacaacag
     4261 caatagcagc gccagccggc gggccagcga cgcggcgcac acgccgcaac acatcgccgg
     4321 gactccgcgc cagctagcca ttcgcctgga cgacgatatt caccagcttg cccggcacca
     4381 cgatcacctt gcgaaccacg ccgtcgccga tgaaccgggt cacctgctcg ttggccagtg
     4441 ccgccgcctc gcaagcctcg cgatcggcat cggcggcgac ctgcatgcgg ccgcgcagct
     4501 tgccgttgac ctgaatcacc atctcgatgc tgtcctgcac cagggccgcg tcgtcgacct
     4561 cgggccacgg atgatcgatc aacggctcct cgtggccgag atcctgccac agccggtgac
     4621 agatatgcgg aatgatcggc gccagcatca acaccatgct ggtaaagact tcgcggcgga
     4681 tcgccgtgcc ggccgcgctg tcatcgtcga aacgcgacac gctgttgagc agttccatgt
     4741 tggcggcgat cgcggtgttg aaggtcaggc ggcgctcgaa atcgtcgctg accttggcga
     4801 tggtttcgtg cagcttgagg cgcatcgcct tttgcgcgtc gtcgagcgcg gcggcatcga
     4861 cggccgtcac ttcgtgagcc tcggcgaggt gctcctgcac cgcggcccag aagcgcttga
     4921 gaaagcggta agcgccctcg acgccgctgt ccgaccagtc cagcgattgc tccggcggcg
     4981 atgcgaacat gatgaacagg cgcacggtgt cggcgccata gcggtcgatc agctcctgcg
     5041 ggtcgatggt gttgcccttc gacttcgaca tcttgctgcc gtccttgagc accatgccct
     5101 gggtcagcag ccgcttgaac ggttcaccgc tctcgagcag gccctcgtcg cgcatcagct
     5161 tgtgatagaa gcgcgcgtac aacaggtgca ggatggcgtg ctcgataccg ccgatataca
     5221 ggtccaccgg cgcccagtaa gcggtgcgct catcgagcat cgcctgctgc tggtccgggc
     5281 aggtgaagcg ctcctggtac caggaagact ccataaaggt atcgaaggta tcggtttcgc
     5341 gctccgccgg cccgccgcat tgcgggcagg tggtgcgaat gaatgactcc atcttcttga
     5401 tcggcgagcc gatgccctcg aactcgacgt cttccggcag caccaccggc agatcgttct
     5461 ccggcaccgg cagctggccg caggcgctgc aattgatcac cgggatcggc gcgccccagt
     5521 agcgctgccg cgacacgccc cagtcgcgca gccggtaatt gatctgccgg gtgcccttgc
     5581 ccgcggactc gagcc
//
LOCUS       DATCZV010000294         7312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_659004, whole genome shotgun sequence.
ACCESSION   DATCZV010000294 DATCZV010000000
VERSION     DATCZV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659004"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VIW27_06530"
     CDS             <1..868
                     /locus_tag="VIW27_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY5739364.1"
                     /translation="VKTLLKGNPGAGRLAETNRLLAEFSASQSDSDYLRALRSSRLAE
                     LIRDWRLDLERGAAEVLYDDPAKITAARGQKIHLLARLDTYFDAIHDELLIFSPYFVP
                     GANGVARLRQLCRRGVRVGVITNSLASTDVAAVHAGYARYRKPLLRAGVELHEIDHTL
                     SRQERHQKRGPRASSKASLHAKSFVIDNKRVFIGSLNLDPRSVVENTEIGIMIESPDM
                     ARLMSRGFEHIARRAAFRVALENPQTAGEALRWHRQSRGGQQVFRSEPNTGFWRRFGA
                     AFMRLLPIESQL"
     gene            complement(1104..1817)
                     /locus_tag="VIW27_06535"
     CDS             complement(1104..1817)
                     /locus_tag="VIW27_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739365.1"
                     /translation="MIRLSSYSVQNADTQLAVANSSSGIGLGYSFNDDLGGEDSVTIP
                     RLDAYYRFSERHRIDFSAFSFKREGRKILQIDVDLGDQTFNIGETLETDIEYNLYKIG
                     YVYTFFHSDRVELGVGAGLNVTSYDLVYRLADGTNGGSADASGPLPMFGFRMSYAINP
                     QWSLHYISEAFYIELDDALKGSFTSSELDIQYRFANSFVLGAGITRFSTDLSADDSEW
                     KGKIVDSHRGLLVYASYYL"
     gene            2139..3095
                     /locus_tag="VIW27_06540"
     CDS             2139..3095
                     /locus_tag="VIW27_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY5739366.1"
                     /translation="MTVRIFIRRSLRCCLLAIPLTIFAPFAPVDAQDAEPGVAADDSD
                     TGSLVKERLERERRVADNRSVLLAHKRNYLLPLTYAVSPNDDVYESGSSDLGTDLDNL
                     EIQFQLSIKAALAEGLFTEHDVLYTGATVRAFWQAYNEDISAPFRETNYEPELFWVTP
                     VPWTILGGDATLFALGLSHQSNGRSQPFSRSWNRLYGSVIWERWRYVFHFKAWWRIPE
                     DEKDDPLDAEGDDNPDIEDYLGNFEFTVAYRKFDHEVSVMLRNNLDREDNRGAIQIDW
                     TFPLQRRFRGYLQFFNGYGESLIDYNAHIERIGVGILLSDLL"
     gene            3172..5475
                     /locus_tag="VIW27_06545"
     CDS             3172..5475
                     /locus_tag="VIW27_06545"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5739367.1"
                     /translation="MTIIRYDYLPTALVSLLLGLVLSMPLPAAAQQSADGETPAVEIP
                     ENLTREEVRDLVARLSDDQVRDLVITQLDKLAVSAQSESQANVYLARLRAGVREARES
                     LRRSLNTREKPLQTLAANAWGQLTADGTDGSRLLLQLLVLLAAGLGADRLARILLRRA
                     SAIAVESLSLSQKFDIACYRAAVGAMGIAAFAVGAMVAVELIAANPAAESLWYQLIWL
                     IVTVRAVMLIVSQIVSPKSSSTRMVELSDAAASEAWIWSLALTLSLAVPKQIGSLARE
                     LGATEDHLLLMGMLLGPVFLLLSIALILRLRKHGTRLIIGNDGSVGILRQGLARVWWL
                     LAIAYIVLIWLMALGARAATGESSLLPGLVSLLLMAAIPYFDRGLKWLIACYFDDDRQ
                     ADAAPDEQAAIDTGAAGPTANEIEDTEQVTAPRPNYYAVALRYGRVLMSVAILAIFIR
                     LWNIDVAAISAQLVGERFARASFDIAITILLTWALWGVIRIAIERKIAEDKGPEGASD
                     EAEAGGVGGTRIGTVLPLIRAFIQITLIVMAVLISLSALGVNIGPLIAGAGVIGLAIG
                     FGAQTLVRDIVSGLFYLIDDAFRVGEYVVIDQIRGTVEKISIRSFQLRHHNGPVHTIP
                     YGEIRSLTNWSRDWAIMKFELRIPFETDIEKVRKIIKKIGQELIEDETFGPLLLAPLK
                     SQGVNRMDDSALIIRCKFTAVPGQQYLIRREAFTRIQKAFEEEGIHFAPRRVLVEATT
                     PKQAIEAAAATIDREGEGQQQSSPDEP"
     gene            complement(5487..6284)
                     /locus_tag="VIW27_06550"
     CDS             complement(5487..6284)
                     /locus_tag="VIW27_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEY5739368.1"
                     /translation="MKKLANPAGPLLLLTMLLAACSVRVEPTIEPAQVSGKVTGSVTY
                     RERIALPPDAVVRVALLDVSRMDVAATLIAETVIVPEHAVPIPFELAYDPKKIDPRMV
                     YAVRATILRGDNYLFVTDTHYPVLTRGHGETADLVLKRSGGGAAAVAPAQLTNTRWLL
                     RTLDGEAIEIGVNQRAPFLQFENQGEAAIAHGYSGCNSFTGRYLVAGATLSFENMIST
                     LRACEDMRVEDRFLGMLGQVAAFRIESTWLILSSDSGELATFEAWYE"
     gene            complement(6343..7173)
                     /locus_tag="VIW27_06555"
     CDS             complement(6343..7173)
                     /locus_tag="VIW27_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5739369.1"
                     /translation="MMRRLLTTLILMLCAGAIPAQDVEREITRVTYDVYRFQNRFHVN
                     MFVITEAGVVVTDPINTEAAAWLKAEIEKITDTPITHLIYSHSHADHASGGTGYGDVP
                     NVIMHRNAPEDIDLVEATVRFDEQLTFEVGEKTFELTYLGPGHGTDLIAIVVRPDDVG
                     FVVDAVSSKRLFYRDFPGADVDDWIAQVRKVDSLDFDILIGGHGAVGVKRDVGLGLAY
                     LEELRAEVLQGLQAGKSVDELKRSVTMEKYRAWANYEQWRALNVEGMARHLQESGAVR
                     "
BASE COUNT         1385 a         2279 c         2252 g         1396 t
ORIGIN      
        1 ggtgaaaacg ctgctcaagg gtaacccggg agccggcagg ctcgctgaga ccaatcggct
       61 gctggccgag ttcagcgcct cgcaaagcga ctccgactac cttcgggcgc tgcgctcgag
      121 caggctggcc gagctgattc gagactggcg cctcgacctg gagcgcggtg ctgccgaggt
      181 actgtacgac gatcccgcca agattaccgc cgcccgcggc cagaaaatcc acctgctcgc
      241 caggctcgat acctatttcg acgcaatcca cgacgaactg ctgatctttt cgccttattt
      301 cgtacccgga gccaacggcg tcgccaggct gcgacagctg tgccggcggg gcgttcgcgt
      361 cggcgtgatt accaattcac tggcctcgac cgacgtcgcc gcggtgcacg ccgggtacgc
      421 ccgctaccgc aagccgctgt tacgcgcggg cgtcgaactc cacgaaatcg accacacgct
      481 gtcgcgccag gaacgccacc aaaagagagg cccacgtgcc tcgtccaagg ccagcctgca
      541 tgccaagtcg ttcgttatcg ataacaagcg agtgttcatc ggctcgctga acctcgatcc
      601 gcgctcggtg gtcgagaata ccgagatcgg catcatgatc gaatcccccg atatggccag
      661 gctgatgagc cgcggtttcg agcacatcgc caggcgggcc gcttttcgtg tcgcgctcga
      721 gaatccgcag acagccggcg aggcattgcg ctggcaccgg caatctaggg gcggtcaaca
      781 ggtatttcgc agcgaaccca acaccggttt ctggcggcgt ttcggcgccg catttatgcg
      841 cctgttgccg atcgagtccc agctctaggc tgccgtttgc acccgcaccc gaggcaatcg
      901 agccaatagg agtattgcgc gccagcctgg agcgacagcc atagccgtct ctgcgggctg
      961 gaccagcaac cgggttctgg ccgggggcca gggggatttc caggcggcgc gtgccgggtt
     1021 gcagctggtc ggcaacccgc catgaagccc ggtgacatct gccgcaatct cgcggctaac
     1081 cgcaggcgcc gggctggttg cgttcagagg tagtagctgg cgtaaaccag caggccgcgg
     1141 tgcgaatcga caatcttgcc cttccattcg ctgtcatcgg cggacagatc ggtgctgaag
     1201 cgggtaattc cggcaccgag gacgaacgaa ttcgcgaagc gatactgaat gtccaactcg
     1261 ctactggtaa acgaaccttt cagcgcatcg tccagctcga tgtagaaggc ctcggaaata
     1321 tagtgcagcg accactgggg attgattgca taagacatgc gaaagccgaa catcggcaac
     1381 ggcccgctgg cgtcggccga cccgccattg gtgccatcgg caagtcgata aaccaaatcg
     1441 tagctggtga cgttgagacc ggcgccgacg cccagttcga cgcgatcgga atggaaaaaa
     1501 gtgtagacgt atccgatctt gtaaaggttg tactcgatgt cggtctcgag ggtttcgccg
     1561 atgttgaaag tctgatcgcc aagatcgacg tcgatctgca gaatctttcg accctcccgt
     1621 ttgaacgaaa aggctgagaa atcgatgcga tgccgctcgc tgaagcggta ataggcgtcg
     1681 agcctcggga tcgtgacact atcctcgccc ccgaggtcat cattaaaact gtagccgaga
     1741 ccgattcccg aactgctgtt ggcgaccgcg agctgggtat cggcattctg cacgctgtac
     1801 gaggacaggc gtatcatgaa cttatccgca aacgccaggt ttccgtgttc ggccgcaatg
     1861 ccggctaacg gcgccgccaa gagggcaaga aggagacagg cgatgccggg ccccaatttc
     1921 gaggaaaatt gtcgcgcagc gcgcataatt cggactctcg gaaacaaaac ggcaatatag
     1981 ctcaaacggc gcgttgaatg aactaccatc tgccgagttc gaggatagcc ttcgattgcc
     2041 ggtcgccggt tgcggcccgg cactattttg cgcattattg gtgtcaaccc aggcgccgac
     2101 ctcgcgcggc cccttgcccc ggcaccgtcg agcaggttat gacagttcgt atattcattc
     2161 gcaggtcgct gcgttgttgc ctgctggcga ttccactgac gatctttgcg cccttcgcgc
     2221 cggtggacgc gcaggacgcg gagcccggcg tggcggccga cgacagcgac accggttcgc
     2281 tggtcaagga gcgtctcgag cgcgaacgcc gagtcgccga caaccgttcg gtgctgctcg
     2341 cgcacaagcg caattacctg ctgccgctga cctacgccgt gagtcccaac gacgatgttt
     2401 acgaatccgg cagcagcgat ctcggcaccg atctcgacaa cctcgagatc cagttccagc
     2461 tgagcatcaa ggcggcactc gccgagggcc tgttcaccga gcacgacgta ctctacaccg
     2521 gcgccacggt gcgcgccttc tggcaggcct ataacgagga catctcggcg ccgtttcgcg
     2581 aaaccaacta cgaaccggaa ctgttctggg tcaccccggt gccctggacc attctcggcg
     2641 gtgacgccac gctgttcgcg ctcggcctgt cgcaccagtc gaacggccgc agccagccgt
     2701 tttcgcgcag ctggaaccgc ctctatggca gcgtgatctg ggagcgttgg cgctacgtgt
     2761 ttcacttcaa ggcctggtgg cgcattcccg aggacgaaaa agacgatccg ctcgatgccg
     2821 agggcgatga caatcccgat atcgaggact atctcggcaa tttcgaattt accgtcgcct
     2881 atcgcaagtt cgaccacgaa gtctcggtca tgttgcgcaa caatctcgac cgcgaagaca
     2941 atcgtggcgc gatccagatc gactggactt ttccgctgca acgccgcttt cgcggttacc
     3001 tgcagttttt caacggctac ggcgaaagcc tgatcgatta caatgcgcac atcgaacgta
     3061 tcggcgtcgg catcctgctc agcgacctgt tatagagtcg gtgcaatcgc agatcgattg
     3121 cgctaaccta gcgccaaaaa tcaaacctcg aatgcattcc cggcggtgaa aatgacaata
     3181 attcgctacg attacctgcc aacagccctg gtctcgttgc tgctcggcct ggtgctgtcg
     3241 atgccgttgc ccgccgcagc gcagcaatcg gccgacggcg aaacgccggc ggtcgagatt
     3301 cccgagaacc tgacccggga ggaagttcgc gacctggtcg cgcgcctgtc cgacgaccag
     3361 gttcgcgacc tggtgataac ccagctcgac aagctcgcgg tgtccgcaca atcggagtcg
     3421 caagcgaacg tttaccttgc ccggctgagg gcgggtgtcc gcgaggcccg cgaatcgctg
     3481 cggcgcagcc tgaacacgcg cgaaaagccg ttacaaacgc tcgccgcgaa cgcctgggga
     3541 caactcaccg cggacggcac cgacggctcc cggctgttgc tgcaattgct ggtcctgcta
     3601 gccgccggcc tgggtgccga ccggttggcc cgcatcctgc tgcgcagggc gtccgccatc
     3661 gcggtggaaa gcctgtcgct gagccagaaa ttcgatatcg cctgttatcg ggccgcggtc
     3721 ggcgcgatgg ggatcgccgc gttcgccgtc ggcgcaatgg tcgccgtcga gttgatcgcc
     3781 gcaaacccgg ccgccgaatc cctgtggtac cagttgatct ggctgatcgt aacagtcagg
     3841 gcggtcatgt tgatcgtgtc gcagatcgtg tcgccgaaat cgtcctcgac gcggatggtg
     3901 gaactgagtg atgccgctgc tagcgaggcc tggatctggt cgctggcgct gaccctgagc
     3961 ctggcggtac cgaaacagat cggcagcctg gctcgcgaac tcggcgccac cgaggatcac
     4021 ctgctgttga tgggcatgct gctcggaccg gttttcctgc tgctatcgat cgcgctgata
     4081 ttgcgcctgc gcaagcacgg cacgcggctt attatcggca acgacggcag cgtcggtatc
     4141 ttgcgccagg gcctggcgcg cgtctggtgg ctgctggcca tcgcctatat cgtcctgatt
     4201 tggctgatgg cgttgggtgc gcgcgccgcg accggcgaat cctcgctgct gccggggctc
     4261 gtcagcctgt tactgatggc cgcgataccc tatttcgatc gcggactgaa atggctgatc
     4321 gcctgttatt tcgatgacga tcgacaggcc gacgctgcgc cggacgagca ggcggcgatc
     4381 gataccggcg cagccgggcc gaccgccaac gagatcgagg atacggagca ggtgaccgcg
     4441 ccgagaccga attattacgc cgtggcgctg cgctacgggc gggtgctgat gagcgtcgcg
     4501 atactggcta tatttattcg cctgtggaac atcgacgttg ccgcgatcag tgcacagctg
     4561 gttggcgagc gtttcgcgcg cgcctccttc gatatcgcca tcaccatctt gttgacctgg
     4621 gcgctgtggg gcgtgatccg catcgccatc gagcgcaaga tcgccgagga caagggtccc
     4681 gagggtgcct ccgacgaggc cgaggccggc ggcgtcggcg gcacacgcat cggcaccgtg
     4741 ctgccgctga tccgcgcctt catccagatt acgctgatcg tgatggcggt gctgatcagc
     4801 ctgtcggcgc tgggcgtcaa tatcggcccg ctgatcgccg gcgccggtgt catcggcctg
     4861 gcgatcggct tcggcgcgca aaccctggtg cgcgatatcg tctccgggct gttctatctg
     4921 atcgatgacg cgttccgcgt cggcgagtac gtggtaatcg accagattcg cggcacagtt
     4981 gaaaaaatct ccatccgctc gttccagtta cgccaccaca acggcccggt gcataccatt
     5041 ccctacggcg aaattcgttc gctaaccaac tggagtcgcg actgggcgat tatgaagttc
     5101 gaactgcgaa ttccgttcga gaccgacatc gagaaggtgc gcaagatcat caagaagatc
     5161 ggccaggagc tgatagagga tgaaaccttc ggcccgctgc tgctggcgcc gctgaagtcg
     5221 cagggtgtca accgcatgga cgactcggca ctgatcattc gctgcaagtt caccgcggtg
     5281 cccggacagc aatacctgat tcggcgcgag gcgttcacgc gcattcagaa ggccttcgag
     5341 gaagaaggta ttcacttcgc gccgcgccgc gtgctggtcg aggcgaccac accgaaacag
     5401 gctatcgagg ccgcggccgc gacgatcgat cgcgagggcg agggccagca gcagagttcg
     5461 cctgacgaac cctgaagccg ccggggctat tcgtaccagg cctcgaaagt cgcgagctcg
     5521 cccgaatcgc tggacagaat cagccaggtg ctttcgatgc gaaacgccgc gacctgcccg
     5581 agcatgccca ggaagcggtc ttcgacacgc atgtcctcgc aggcccgcag cgtcgaaatc
     5641 atattctcga aactgagggt ggcacctgcc accaggtagc ggccggtaaa actattgcaa
     5701 ccgctatagc cgtgggcaat cgccgcctcg ccctggtttt cgaattgcag gaacggcgcg
     5761 cgctgattga cgccgatctc gattgcctcg ccgtcgaggg tcctgagcag ccagcgggta
     5821 ttggtcagtt gcgcgggggc gacggcagcc gcaccgccac cgctgcgttt cagcaccagg
     5881 tccgctgttt cgccgtggcc tcgcgtgagt acgggataat gggtatcggt gacgaacagg
     5941 taattgtcgc cccgcaaaat cgtcgcgcgc accgcataga ccatgcgcgg atcgatcttt
     6001 ttgggatcgt aggcaagctc gaacgggatg ggtacggcgt gctcggggac gatgacggtc
     6061 tcggcgatca gcgtcgcggc cacgtccatt ctcgacacgt ctagcaatgc cacacgcact
     6121 acggcatcgg gcggcagcgc gatgcgttcg cggtaggtga cgctgccggt gaccttgccg
     6181 ctgacttgcg ccggctcgat cgtcggctcg acgcgcacgc tgcaggccgc cagcaacatc
     6241 gtcagcagca gtaacgggcc cgccggattc gctaacttct tcattgtcgt agtctccggg
     6301 taaaacggtc gcaactcgtt acggccgcag catcgcgccg cttcaacgga cggcgccgga
     6361 ttcctgcagg tggcgcgcca tgccctcgac gttgagtgcg cgccattgct cgtagttcgc
     6421 ccaggcgcgg tatttttcca tggtcacgct gcgtttgagc tcgtcgaccg acttgccggc
     6481 ctgcaaaccc tgcaatactt cggcgcgcaa ttcctcgagg taagcgaggc cgaggccgac
     6541 gtcgcgcttg acaccgaccg cgccgtggcc gccgatcagg atatcgaaat cgaggctgtc
     6601 gaccttgcgc acctgcgcga tccagtcgtc gacgtcggcg cccggaaaat cgcggtagaa
     6661 cagccgcttc gagctgaccg cgtcgaccac gaagccgacg tcgtcgggac gcaccacgat
     6721 agcgatcaaa tcggtgccat ggccgggccc gagataagtc agttcgaaag ttttctcgcc
     6781 gacctcgaaa gtcagctgtt cgtcgaagcg caccgtcgcc tcgaccaggt cgatgtcttc
     6841 gggcgcgttg cggtgcatga tcacgttggg cacgtcgccg tagccggtac cgccggaagc
     6901 gtgatcggcg tggctgtggc tgtagatcag gtgggtgatc ggggtgtcgg tgatcttttc
     6961 aatttccgcc ttcagccagg ccgcggcttc ggtattgatc ggatcggtga ccaccacgcc
     7021 ggcctcggta atcacgaaca tgttgacgtg aaagcggttc tgaaagcggt agacatcgta
     7081 ggtcacccgc gtaatttcgc gctcgacgtc ctgcgccggg atggcaccgg cgcaaagcat
     7141 caggatcagg gtggtaagca atcgccgcat catcggtcct cctcgagtca tcgccgccac
     7201 atgatatctc gaatcggctt tttctgcttt aatgccgggc tatgaaattg ctcgaatccc
     7261 gcctgcggct ggccagcggc ctgtatctcg ccgcctacgt gctgcaacac ct
//
LOCUS       DATCZV010000295         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_554134, whole genome shotgun sequence.
ACCESSION   DATCZV010000295 DATCZV010000000
VERSION     DATCZV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554134"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VIW27_06560"
     CDS             <1..628
                     /locus_tag="VIW27_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5739370.1"
                     /translation="NALLESLLADGHRAFIQQADVADEQQVMAMFEAIDQRFGRLDAL
                     VNTAGILEPHCDLVEMSPQRWRRVLDVNVVGSFLCAREAVRRMATTSGGNGGAIVNVS
                     SRAADLGSPHEYVDYAAAKGAVDSMTIGLAKEVAPLGIRVNAVKPGLIYTDIHAAGGE
                     AGRVDRLKVGVPMQRGGQPSEVAEAIVWLLSDASAYTTAGFIDVSGGR"
     gene            628..>2324
                     /locus_tag="VIW27_06565"
     CDS             628..>2324
                     /locus_tag="VIW27_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEY5739371.1"
                     /translation="MPRHNPASIPLLSLNAVAFDSETTGLDTSRARMIQLGAVRIVHG
                     EVDSAQSFQQLINPGVPIPDDSREIHGISDADVADAPAFTEVVEAFDQWRGDAVLVGY
                     ASGFDLAMLKREREIAGLGWTAPRCLDVRFLVNLLAPNLPDFSLDTIASWAGVETHNR
                     HSALGDSIATAEIFVALIPRLRETGIRTLAELERACERFSETATQESRIGWLDVHEAR
                     RSRAVLARIDSFPYRHRLRDLMRSPPLFIDADIALHKALEQMIDAQVSSLYVNPRDAD
                     GPVGIVTERDLLREFRRHGSAAFDTPVHSVAVFPLESLRDEAFVYRAIARMKRLHIRH
                     LGVHDKDGRIVGALSARDLLRQRADDAVLLGDDIDAAENTGQMAAVWGNIVLVAKGLV
                     AEEVDARDIAAVVSRELCALTRQACRLAERKMETPPPCAYAMLVLGSGGRGESLLAMD
                     QDNAIVFAEGEPGGAEDQWFAELGKRVSDMLDAAGVPYCKGKVMASNADWRHSVAGWE
                     NLVGTWIRRQNPEDILNCDIFFDAVPVHGDLELANRVLDRAFELGRSSSIFIKLMSI"
BASE COUNT          394 a          764 c          764 g          402 t
ORIGIN      
        1 gaacgcgttg ctggaatcgc tgctcgccga cggccaccgg gctttcatcc agcaggccga
       61 tgtcgccgac gaacagcagg tgatggccat gttcgaagcc atcgaccagc ggttcgggcg
      121 cctcgacgca ctggtcaaca ccgccggcat actggagccg cattgcgatc tggtggaaat
      181 gtcgccgcag cgctggcggc gtgttctcga cgtcaatgtc gtcggcagct ttttgtgcgc
      241 gcgcgaggcg gtcaggcgca tggccaccac cagcggcggc aacggcgggg cgatcgtcaa
      301 tgtatcgtca cgcgccgccg acctcggcag cccgcacgag tatgtcgatt acgccgccgc
      361 caagggcgcc gtcgattcga tgactatcgg gttggccaag gaagtggcgc cgctgggtat
      421 tcgcgtcaat gcggtcaagc ccggcctgat ctataccgat attcatgcgg cgggcggcga
      481 ggccggccgc gtcgaccgtc tcaaggtcgg cgtgccgatg cagcgcggcg ggcagccgag
      541 cgaagttgcc gaggccattg tgtggctgct gtcggatgcc tcggcgtata ccaccgccgg
      601 cttcatcgat gtatccggag gccgctgatg ccgcgacata acccggcctc gattccactg
      661 ctgtcgctga acgcggtcgc cttcgacagc gagaccaccg ggctcgatac ttcgcgcgcg
      721 cgcatgatcc agctcggggc cgtgcgcatc gtacatggcg aggtcgatag cgcgcagagc
      781 tttcaacaat tgatcaatcc cggggtgccg atccccgacg acagccgcga aatccatggc
      841 atcagcgacg ccgacgtcgc cgacgcgccg gcattcaccg aggtcgtcga agccttcgac
      901 cagtggcgcg gcgacgcggt actggtcggc tacgccagcg gcttcgatct ggcgatgctc
      961 aagcgcgagc gcgaaatcgc cgggctcggc tggactgcgc cgcgttgtct cgacgtccgc
     1021 ttcctggtca acctgctggc gcccaatctg ccggattttt cgctcgacac gatcgccagc
     1081 tgggccgggg tcgaaactca caatcgccac agtgcgctcg gcgattcgat cgccaccgcg
     1141 gaaattttcg tcgcgctgat cccgcgcctg cgcgagaccg gcattcgcac cctggccgaa
     1201 ctcgaacgag cctgtgaacg tttcagcgaa accgccaccc aggaaagtcg aatcggctgg
     1261 ctcgacgtcc acgaggcgcg gcgctcgcgc gcggtgctgg cgcgcatcga cagctttccg
     1321 tatcgccacc gcttgcgcga cctgatgcgc agtccgccgc tgttcatcga tgccgatatc
     1381 gcgctgcaca aggcgctcga acaaatgatc gacgcccagg tcagttcgct gtacgtcaat
     1441 ccgcgcgacg ccgacggccc ggtcggcatc gtcaccgagc gcgacctgtt gcgcgagttc
     1501 cgccgccacg gcagcgcggc cttcgacacg ccggtacatt cggtggccgt gttcccgctc
     1561 gaaagcctgc gtgacgaagc cttcgtctac cgcgccatcg cgcgcatgaa acggttgcat
     1621 attcgccacc ttggcgtgca cgacaaggac ggtcgtatcg tcggagcgct gtcggcgcgc
     1681 gacctgctgc ggcagcgcgc cgatgacgcg gtgctgctcg gcgacgatat cgacgccgcc
     1741 gaaaacaccg gccagatggc cgcggtctgg ggcaacatcg tgctggtggc gaaaggcctg
     1801 gtagccgagg aagtcgacgc gcgcgacatc gccgcggtag tgtctcgcga actctgcgca
     1861 ctgacccgcc aggcgtgccg gctggcggag cgcaaaatgg agacaccgcc gccctgcgct
     1921 tacgcgatgc tggtgctggg ctccggcggt cgcggcgaaa gcctgctggc aatggaccag
     1981 gacaacgcca tcgtgtttgc cgagggtgag ccgggcggcg ccgaggacca gtggttcgcc
     2041 gaactcggca agcgggtttc ggatatgctc gacgccgctg gcgtgcccta ctgcaagggc
     2101 aaggtcatgg ccagcaacgc ggactggcga cactcggtcg cgggctggga aaacctggtc
     2161 ggtacctgga ttcggcgcca gaaccccgag gatattctca attgcgatat ctttttcgac
     2221 gcggtgccgg tccacggcga tttagagctc gccaatcgcg tgctcgaccg ggctttcgag
     2281 ctcgggcgca gctcgagcat tttcatcaag ctgatgtcga tcaa
//
LOCUS       DATCZV010000296         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_447350, whole genome shotgun sequence.
ACCESSION   DATCZV010000296 DATCZV010000000
VERSION     DATCZV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447350"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VIW27_06570"
     CDS             <1..637
                     /locus_tag="VIW27_06570"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739372.1"
                     /translation="IDEQPLARPGMGAWQRIGAAFGELRDTLREVRQYRRAAIFLLAY
                     FLYMDGVYTVIKMAVAYSNALGFDSMVPMKGILAVQVIAIPATLAFGRLAGRFGARRL
                     IMVGVFAYLVVTGGAPFMTQPWHFYILAVIIGLAQGGLQSLSRSYYAQLIPQRESGKY
                     FGFYNMLGKASAVLGPFMMGGLALVLNEQLSILAIPVLLVGGLIVLFRLGD"
     gene            complement(764..>2053)
                     /locus_tag="VIW27_06575"
     CDS             complement(764..>2053)
                     /locus_tag="VIW27_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5739373.1"
                     /translation="AAQRDGDPQRGRQLVVNGSYMSCGLPYRAWQRAAGGGDPGPQPR
                     GREPRNAGLPYALSVNSNADGVDVVAANCLLCHAAPFDGELVIGLGNEALDFSGDSRG
                     LVNAVGAYVSGAAESAAWRQWADRVAAIAPYSITDTIGVNPATNITLALIAHHDPQTL
                     AWSNQPLIEPPPKTPLPVSVPPWWRVGKKHAMFYNAMGRGDHARYMMMKSLLCANSVD
                     AAESADAEFVHVRAFLASLQPPRYPWPIDADLAQRGKTVFEQSCAGCHGTYGADGSYP
                     NLVVDLATIGTDPAYARSSYDAERFMDWFNRSWYGERASARPAPGYIAPPLDGVWATA
                     PFLHNGSVPTIAALLDSRTRPRYWTRDFEAPEFDAEALGWKHRSLPRGKQATADPLER
                     KRIYDTTLHGYSNAGHLFGDALSAEQRQALLEYLKTL"
BASE COUNT          302 a          648 c          730 g          373 t
ORIGIN      
        1 gatcgacgaa caaccgctgg ccaggccggg catgggcgcc tggcagcgca tcggcgccgc
       61 cttcggcgaa ttgcgcgaca cgctgcgcga ggtcaggcaa taccgcaggg cggcgatttt
      121 cctgctggcc tatttccttt acatggatgg tgtttacacc gtgatcaaga tggctgtcgc
      181 ttactcgaat gcgctggggt tcgattcgat ggtgccgatg aagggcattc tggcggtgca
      241 ggtcatcgcc ataccggcga cgctggcatt cggcaggctg gccggccgat tcggcgcccg
      301 gcgcttgatc atggtcggcg tgttcgctta cctggtggtg accggcggcg ctcccttcat
      361 gacacaaccc tggcacttct acatcctcgc ggtgatcatc ggtctggcac agggcggcct
      421 gcaatcgttg agccgctcgt attacgcgca gctcatcccg caacgggaat cgggcaagta
      481 cttcggcttt tacaacatgc tcggcaaggc ttccgcggta ctgggaccct tcatgatggg
      541 tggcctggcg ctggtattga acgagcagct gtcgattctg gcgatcccgg tgctgttggt
      601 cggcggattg atcgtattgt tccggctcgg cgactgagcg ggggcgcagg tcagcgctgc
      661 gtggatttac ggcggttcgc gctgccgatc gcggattggg cgctgcggcg atgccgagca
      721 cacccggccg cgaatccgca gcgaccgccc gaacgactcg ccgttacagg gttttcaggt
      781 actcgagtag cgcctgccgt tgctcggcgg acaaggcgtc gccgaacagg tgcccggcgt
      841 tcgagtagcc gtgcagcgtg gtatcgtaga tgcgcttgcg ctcgagcgga tcggcggtcg
      901 cctgtttgcc gcgcggcagg ctgcggtgtt tccagccgag tgcctcggca tcgaactccg
      961 gtgcctcgaa atcccgcgtc cagtagcgcg gccgcgtgcg gctgtcgagc agcgcggcga
     1021 tcgtcggcac cgagccgttg tgcagaaatg gcgccgtcgc ccagaccccg tcgagcggcg
     1081 gcgcgatgta tcccggcgcc ggcctggcgc tggcccgttc gccgtaccag gagcgattga
     1141 accagtccat gaagcgttcg gcgtcgtagg agctgcgcgc gtaagccggg tcggtaccga
     1201 tcgtcgcgag gtcgacgacg aggttcgggt agctgccgtc ggcgccatag gtgccgtggc
     1261 agccggcgca gctttgctcg aacacggttt tgccccgttg cgcgagatcg gcatcgatcg
     1321 gccacgggta gcgcggcggc tgcagcgagg cgaggaaagc gcgcacgtgg acgaactcgg
     1381 catcggccga ttcggccgcg tcgacgctat tggcgcacag cagcgacttc atcatcatgt
     1441 agcgcgcgtg atcgccgcgg cccatcgcgt tgtagaacat cgcatgcttc ttgccgacgc
     1501 gccaccacgg cggcacgctg accggcagcg gcgttttcgg cggcggctcg atgagcggct
     1561 ggttcgacca ggccagggtt tgcggatcgt ggtgggcgat cagcgccagc gtgatattgg
     1621 ttgccgggtt gacgccgatg gtatcggtga tcgagtaggg ggcgatcgcc gcgacgcggt
     1681 ccgcccactg gcgccaggcg gcgctctcgg ccgcgccgga aacataggcg ccgaccgcgt
     1741 tgaccaggcc gcgcgaatca ccgctaaagt cgagcgcttc gttgccgagg ccgatcacca
     1801 gttcgccgtc gaagggcgcc gcgtggcaca gcaggcaatt ggcggcgacc acgtcgacgc
     1861 cgtcggcgtt ggagttgacg ctgagcgcgt aaggcagccc ggcattgcgc ggctcgcggc
     1921 cacgcggttg cggaccgggg tcgccgccgc cggcggcccg ctgccacgcc cggtacggca
     1981 gtccgcaact catgtaactg ccgttgacca ccagttgccg gccgcgctgc ggatcgccgt
     2041 cgcgctgggc ggc
//
LOCUS       DATCZV010000297         3203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_235512, whole genome shotgun sequence.
ACCESSION   DATCZV010000297 DATCZV010000000
VERSION     DATCZV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235512"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /gene="fmt"
                     /locus_tag="VIW27_06580"
     CDS             complement(<1..775)
                     /gene="fmt"
                     /locus_tag="VIW27_06580"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5739374.1"
                     /translation="MLKIVYAGTPEFAVPALAALAASEHRLVAVYTQPDRPAGRGRKL
                     QPSPVKCCALRHGIEVHQPADFKDSAALEALNVLQADLMVVAAYGLLLPAAVLEAPRL
                     GCINLHASLLPRWRGASPIQQAILAGDARSGITLMKMDVGLDTGDMVAHREIDIDPGW
                     SAGELHDALAPLAADLLMDTLPAIESALAAALPQDESLACYAPRLDKRQAKIDWEKSR
                     LQLVREVLAYNPWPVSYTRLENDNLRLWRAASGDASEGGR"
     gene            complement(789..1295)
                     /gene="def"
                     /locus_tag="VIW27_06585"
     CDS             complement(789..1295)
                     /gene="def"
                     /locus_tag="VIW27_06585"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5739375.1"
                     /translation="MALLNILHFPDPRLRTVARPVTEFDDDLRRLVGDMFETMYEAPG
                     IGLAATQVDRHIRLLVLDVSEGRNRPRCLINPRILEADGEEEMDEGCLSVPGFYEKVQ
                     RADHIRVRAQDEHGEFHEFEASGLEAVCIQHEMDHLEGKLFVDYLSGLKRNRIRNKLL
                     KAQKQAAD"
     gene            1436..2620
                     /locus_tag="VIW27_06590"
     CDS             1436..2620
                     /locus_tag="VIW27_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY5739376.1"
                     /translation="MPANNNLRQSKGFFLLAGIMLLVACAEPPPPYAVVYEPPPPQEN
                     VVVKAAVEQPRPVETIVYEPEYPETYIVQEGDTLWDISTVFLRDPWYWPEIWFKNPQI
                     ENPHLIYPGDTLAIIYVGGQRRVQLISRGEQGQLQQGQTAEGLQIVKVNPRVRTQPID
                     ATIPTIQIDRIRHLIARPMVIDPETLNNSAYVLASLDDHLINSINDKLYVRKLDTSSG
                     SGRYQIYRPNRPIYDPDTSELLGYEAIYVGESKLLLRGDPASVRVTNSEREILRDDRV
                     MPIDNTAFERDFYPRPPNSEVSGRIVALLDAISQTGLYQTIAINLGQRDGLETGNILR
                     ISRIGVAIPDPAEPDPRFRVKLPDEQIGMAMIIRTFEKMSYALVMEADFPITLNDYVE
                     SP"
     gene            2715..>3203
                     /locus_tag="VIW27_06595"
     CDS             2715..>3203
                     /locus_tag="VIW27_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY5739377.1"
                     /translation="MRKDLLPDFLHLFHAFGNHRGALLRLLDQCGGDPSRARRSDPGE
                     LRELGFGARAVACVAKTFERLVETDLDWASQAAHRLICYDDEAYPERLRQIADFPLLL
                     YARGDTRLLRSPQIAVVGSRRCTPGGARTAFEFADGLAAAGLTVTSGLALGIDTEAHR
                     GAL"
BASE COUNT          603 a         1062 c          950 g          588 t
ORIGIN      
        1 gccgcccgcc ttcactggca tcgccgctgg cggcccgcca caggcgcagg ttgtcgtttt
       61 cgagccgggt gtagctgacc ggccacgggt tgtaggcaag cacctcgcgc accagttgca
      121 ggcgggattt ttcccagtcg atcttcgcct gccgcttgtc gagtcgcggc gcgtagcagg
      181 cgaggctctc gtcctgcggc agcgcggccg ccagcgccga ctcgatggcg ggcagcgtat
      241 ccatcagcag gtcggccgcc aacggcgcca gcgcgtcatg caactcgccg gcgctccacc
      301 ccggatcgat gtcgatttcg cggtgggcga ccatgtcgcc ggtatccagc ccgacgtcca
      361 tcttcatcag cgtaatcccg ctgcgcgcat cgccggcgag aatcgcctgc tggatcggtg
      421 acgcaccacg ccagcgcggc agcagcgagg cgtgcaggtt gatacagccg agccgcggcg
      481 cttcgagcac cgcggccggc aacagcaatc cataggccgc gaccaccatc agatcggcct
      541 gcaggacgtt caatgcctcg agcgcagcgg agtccttgaa atcggcgggt tgatgcacct
      601 cgatgccgtg ccgcagcgcg cagcacttga ccgggctcgg ctgcagcttg cggccgcggc
      661 cggccggccg gtcgggctgg gtatataccg cgaccaggcg gtgctcgctg gcggccaggg
      721 cggcgagcgc cggcacggcg aattccgggg ttccggcata gacgattttc aacatgccgg
      781 agcagcggct agtcagccgc ctgcttttgc gccttcagca acttgttacg aatccggttg
      841 cgcttcagcc cggacaggta gtcgacgaac agcttgccct cgagatggtc catctcgtgc
      901 tgtatgcata ccgcttcgag gccgctggct tcgaactcgt ggaactcgcc gtgctcgtcc
      961 tgcgcgcgca ctcgaatgtg gtcggcacgc tgcacctttt cgtagaagcc cggcaccgac
     1021 aggcagccct cgtccatttc ctcctcgccg tcggcttcga gtattcgcgg gttgatcagg
     1081 cagcgcggac ggttgcggcc ctcggagacg tccagcacca gcagtcgaat atggcgatcg
     1141 acctgggtcg cggcgaggcc gattcccggc gcttcgtaca tggtttcgaa catgtcgccg
     1201 accagccggc gcaagtcatc gtcgaactcg gtgaccggcc tggcgacggt tctcaaccgc
     1261 ggatcgggga aatgtaaaat attcagtaaa gccatcttta taaaacttca agcaagattg
     1321 gaactagtcg ttccaagtta atgattatat taatatcttc aaggccaaat agcttgtagt
     1381 cggttttttt tgagctacaa tccacgaatt agtgccaaat atttcagggg ttcgtatgcc
     1441 tgccaacaat aatctacggc aatcaaaggg gttctttctg ctggcgggaa tcatgctgct
     1501 cgtcgcctgc gccgagccgc cgcctcccta tgcggtcgtc tacgaacccc cgccgccgca
     1561 ggaaaatgtc gtcgtcaagg cagccgtcga acaacccagg ccggtggaaa ccatcgtcta
     1621 cgaacccgaa tatcccgaaa cctacattgt acaagaaggc gatacgctat gggatatctc
     1681 gacagttttt ctgcgcgatc cctggtactg gccggaaatc tggttcaaga atccgcaaat
     1741 cgaaaatccg cacctgatct accccggcga tacgctggcg atcatctacg tcggcggcca
     1801 gcggcgcgtg caactcatca gccgcggcga gcaggggcaa ctgcagcagg gccagaccgc
     1861 cgagggactg caaatcgtca aggtcaaccc gcgagtgcgc acccagccga tcgacgccac
     1921 catcccgacc atccagatcg atcgcattcg tcacctgatc gcgcggccga tggttatcga
     1981 tcccgaaacc ctgaataact cggcctacgt gctggcgagc ctcgacgatc acctgatcaa
     2041 cagcatcaac gacaagctct acgtgcgcaa gctggatact tcgagcggca gcggccggta
     2101 tcagatctac cgtcccaacc gcccgatcta cgaccccgat accagcgaac tgctcggcta
     2161 cgaagcgata tacgttggcg aatccaagct gctgctgcgc ggcgacccgg catcggtcag
     2221 ggtgaccaat tccgagcgcg aaatcctgcg cgacgatcgc gtcatgccga tcgacaacac
     2281 cgcgttcgag cgcgattttt atccccggcc gccgaattcc gaagtcagcg gccgcatcgt
     2341 cgccctgctc gatgcgattt cgcaaaccgg tctctaccag accatcgcca tcaacctcgg
     2401 gcaacgcgac ggcctcgaaa ccggtaacat cctgcgcatc agccgcatcg gcgtagcgat
     2461 tcccgatccc gccgagccgg atccgcggtt tcgcgtcaag ctgccggacg aacaaatcgg
     2521 catggcgatg atcatccgca cctttgaaaa aatgagctac gcgctggtca tggaagccga
     2581 ttttccgatc acgctcaacg actacgtcga atcgccctga tccgaccgtc ggctcgaagc
     2641 catgccggca actggcagcg ggcggtgccc cggtgctata gtgcaagggt ctgcaaacgg
     2701 aactggagac cgtcatgcgc aaggatttgc tgcccgactt cctgcacctg tttcacgcct
     2761 tcggcaacca tcgcggcgcg ctgctgcggc tgctcgatca gtgcggcggc gacccgtcgc
     2821 gcgcccgtcg cagcgatccc ggcgaattgc gcgaactcgg cttcggtgca cgcgccgtcg
     2881 cctgcgtcgc gaaaaccttc gagcgcctgg tcgagaccga tctcgactgg gcatcgcagg
     2941 ccgcccatcg gctgatctgt tacgatgacg aggcctatcc cgagcgcttg cgccagatcg
     3001 cagatttccc gctgctgctg tatgcccgtg gcgatacccg gctgttgcgc agcccgcaga
     3061 tcgcggtcgt cggcagtcgc cggtgcacgc cgggcggtgc gcgcacggca ttcgaattcg
     3121 ccgacgggct ggccgcggcc ggcctcaccg tgaccagcgg cctggcgctc ggcatcgaca
     3181 ccgaggcaca tcgcggtgcg ctg
//
LOCUS       DATCZV010000298         8815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_288143, whole genome shotgun sequence.
ACCESSION   DATCZV010000298 DATCZV010000000
VERSION     DATCZV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288143"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1097)
                     /gene="hisF"
                     /locus_tag="VIW27_06600"
     CDS             complement(318..1097)
                     /gene="hisF"
                     /locus_tag="VIW27_06600"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY5739378.1"
                     /translation="MSLAKRIIPCLDVDAGRVVKGVRFVDIRDAGDPVEVARRYNREG
                     ADELTFLDITASSDDRETMAHVVEAVAREVFIPLTVGGGIRELADVRRMLNAGADKVS
                     INTAAVFNPEFVAEAADKFGSQCIVVAVDAKRAEDGRFEVFTHGGREPTGIDAIDWAR
                     RVVALGAGEILLTSMDRDGTKAGYDIPLTRAVADAVSVPVIASGGVGTLDHLVEGVRE
                     GHAAALLAASIFHFGTYSIGEAKAHLAAHGVPVRPPEPLEV"
     gene            complement(1116..1847)
                     /gene="hisA"
                     /locus_tag="VIW27_06605"
     CDS             complement(1116..1847)
                     /gene="hisA"
                     /locus_tag="VIW27_06605"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY5739379.1"
                     /translation="MILIPAIDLKEGHCVRLRQGNMEETTVFSDDPVAMAGKWVTQGC
                     KRLHLVDLDGAFEGLPINADVIEEICASFPNLVIQIGGGIRSIDTVEAYLEAGVDFVI
                     IGTMAVKRPEFITQLCSEFPGQVIIGLDARNGMVATHGWEEESEHSALEFALRFEDQG
                     VSAIVYTDISRDGMMQGVNVEATKKLAAAISVPVIASGGVTDMDCIVKLNAVKLSGIE
                     GVIIGRALYERTIDLVEAQAYVDSH"
     gene            complement(1844..2491)
                     /gene="hisH"
                     /locus_tag="VIW27_06610"
     CDS             complement(1844..2491)
                     /gene="hisH"
                     /locus_tag="VIW27_06610"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY5739380.1"
                     /translation="MQTIAVVDYGMGNLRSVVNALEAVSEARARVLLTSDPRQIRGAD
                     RVVFPGQGAAGDCMRAIDDYGLREAILDAAGQRPFLGICMGLQVLIEYSEENRGTDCL
                     AYLPGRVRYFGDDHRDADGVRLKVPHMGWNRVRRTREHPLWRSINDGSRFYFVHSYYL
                     DEPDGELVAAQTDYGFAFTSAIARDNLFALQCHPEKSADAGLQLLRNFVAWNGQS"
     gene            complement(2495..3088)
                     /gene="hisB"
                     /locus_tag="VIW27_06615"
     CDS             complement(2495..3088)
                     /gene="hisB"
                     /locus_tag="VIW27_06615"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5739381.1"
                     /translation="MSDRSATVSRNTLETQIEVSLNLDGSGRSSFATGIPFLEHMLEQ
                     IARHGAFDLDIKASGDLHIDDHHTVEDIGITLGQAFKQALGDKQGILRYGHAYVPLDE
                     ALSRVVIDFSGRPGIEYRVDFRRPRVGQFDVDLIHEFFQGFANHAPATLHIDNLAGDN
                     AHHIAETIFKAFGRALRVAVARDERMPGAVPSTKGSL"
     gene            complement(3170..3604)
                     /gene="dtd"
                     /locus_tag="VIW27_06620"
     CDS             complement(3170..3604)
                     /gene="dtd"
                     /locus_tag="VIW27_06620"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018994438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY5739382.1"
                     /translation="MITLLQRVSEARVEVDGQVVGAIGRGLLVLAGVRAQDDEASARA
                     AIERLLNYRVFADADGKMNLSLRDVEGGLLLVPQFTLAADTRKGRRPSFSSAASPQRG
                     QQLFELALDHARAVHQPVASGRFGADMRVQLINDGPVTFILD"
     gene            complement(3601..4551)
                     /gene="pip"
                     /locus_tag="VIW27_06625"
     CDS             complement(3601..4551)
                     /gene="pip"
                     /locus_tag="VIW27_06625"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY5739383.1"
                     /translation="MRMLFPAIQPGQTWRFPVSDLHTLYLEECGNPNGVPIVFLHGGP
                     GGSCEPGHRRFFDPESYRIILFDQRGAGKSRPHASLENNTTPELIADLEKIREFLKIE
                     RWVVFGGSWGSTLALAYAQTHPERVMGLILRGIFLARERDVDWFFGGHAARVYPEAWE
                     HFIEPIPPEERDDIIGAYYRRLTSKNEIVRMGAAKAWSIWEGSSVTLLPDKSVVEHFA
                     DPHIALSIARIECHYFTNHCFLRPNQLLEDMERIAHIPGFIVHGRYDMVCPVDQALLL
                     KERWQQAELRIVSDAGHAVTEQGISKALVESCNAMLEVVA"
     gene            complement(4842..5630)
                     /gene="ttcA"
                     /locus_tag="VIW27_06630"
     CDS             complement(4842..5630)
                     /gene="ttcA"
                     /locus_tag="VIW27_06630"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017008415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY5739384.1"
                     /translation="MEIDSSGLSRDLESLQRRIRHGAGRAVAEFEMIADGDRVMVCLS
                     GGADSYTLLDVLLGLQKAAPIDFELVAVNLDQKQPGFPERVLPEYLESLGVEYRIVEE
                     DTYSVVKRVIPEGKTTCSLCSRLRRGVLYNTASEIGATRIALGHHADDIVETAFLNMF
                     FNSRLKAMPAKLRSDDGRHVVIRPLAYLREVLIKQYAALRRFPIIPCNLCGSQPNLQR
                     QAIKAMLREWDLSHPGRVDNILRSLCRVTPSHLLDRELYDFGNL"
     gene            complement(5650..7101)
                     /locus_tag="VIW27_06635"
     CDS             complement(5650..7101)
                     /locus_tag="VIW27_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEY5739385.1"
                     /translation="MQPLVILRILGLLMLLSSCTLLPPIATGLIFGDGAVLPFVEAFA
                     LTLVSGFLLYLPVRKHRNELRLRDGFLVVVLFWTVLGVAGGLPIYLSGVQDISITDAV
                     FESISGITTTGATVIVGIDELPHSLLLYRQELQWLGGMGIIVLAVAVLPMLGVGGMQL
                     FRAETPGPVKDTKLTPRITETAKALWYIYLGLTVACLLAYWAAGMSFFDAMSHSFSTV
                     AIGGFSTHDASIGHFDSTAIQFVAIVFMLLSGINFAIHFTAFRNRSLKPYRRDAEFRT
                     YMLVLILVSIVTVGYLQLTHTFDSTTESIIEGLFQVVSIGTTTGYTTAEYYTWPGFLP
                     VLLLYVSFIGGCSGSTGGGIKVIRILLLVKQGVRELRRLVHPNAQFAVKIGKKAMPEK
                     VIEAVWGFFAAYFAISALLILLLMATGLDQVTAFSAVAACLNNLGPGLGEVGRNFASI
                     NDPAKWILCVAMLLGRLEIFTLLVLFSPAFWRK"
     gene            complement(7121..8497)
                     /gene="trkA"
                     /locus_tag="VIW27_06640"
     CDS             complement(7121..8497)
                     /gene="trkA"
                     /locus_tag="VIW27_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEY5739386.1"
                     /translation="MNIIILGAGQVGSTVATELAKEESNEITVIDTNQQILSDLQDRL
                     DLRTICGNGSYPGLLEDAGADDADMLIALTNSDEINMIACQIASSLFHTPTKIARIRS
                     PEYLGRPGLFAEESIPVDVMISPESLVTDYIYHLIEHPSALQVLDFAKGRAQLVAVKA
                     YDDGLLVGHELRTTTEHLKGVDMRVAAIFRDGQPIQPTAECVIEAGDEVFFFAAREHI
                     PKVISEMRKLDKPNKRIMLAGGGNIGGQLAARMENAYQVKVIEAFPARARELSAMLDS
                     AIVLLGDAADPDLLQEENIESMDVFCALTNDDEANILSSMLAKRMGARKVMSLINRPA
                     YVDLVESGLIDIAISPHQVTIGALLAHIRRGDVVAVHALRRGSAEAIEAIAHGDRSSS
                     RVVGRAIEEIKLPEGARIGAIVRGEEVIIAHHDTVIESEDHVILFLTDKKRIGQVEKL
                     FQVGITYF"
     gene            complement(8504..>8815)
                     /locus_tag="VIW27_06645"
     CDS             complement(8504..>8815)
                     /locus_tag="VIW27_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5739387.1"
                     /translation="KQFIRAILTNGDAEEIGLDEIRRVLRQFDTEGERETETGDKMQI
                     TIDMNLELREAREAFEREYLSRQLAASGNNMTELARRVGQERTHLYRKLKSLGLQTKK
                     Q"
BASE COUNT         1711 a         2778 c         2712 g         1614 t
ORIGIN      
        1 ggacaccctc cggcaaccca cccaattcgc ttccaaagac gttattcatc cgacaaatcc
       61 catcaggaaa tcgagctatt cctcgtaacc cggttgacgc gtatagcagt cagagaatgg
      121 cacgcaagtc ccagattgcc ggacgataaa ccagatcgct gccggcatag ggaagtccga
      181 gttgggtcta ccttctaatt ttggcgggta gcaggggcgg cgatagccgg ctcatctgcc
      241 gccggcgggg gtgtggccgt ttgggtcagc gttcaaggac cgaatgcggc acgactgaaa
      301 acccaacacc gccctcctca gacctcgagg ggctccggcg ggcggacggg gacgccgtgg
      361 gcggccaggt gggccttggc ctcgccgatg gaataggttc cgaagtggaa gatcgaggcg
      421 gccaggagcg ccgccgcgtg gccctcgcgt acgccctcga ccaggtgatc gagggtgccg
      481 accccgcccg aggcgatgac cgggaccgag accgcgtcgg cgacagcccg ggtcagcggg
      541 atgtcgtagc ccgcttttgt gccgtcccgg tccatggacg tgagcaggat ctcgccggcg
      601 ccgagcgcca cgacgcggcg cgcccagtcg atcgcatcga tcccggtcgg ctcgcgcccg
      661 ccgtgggtga agacttcgaa gcggccgtcc tcggcccgct tggcgtcgac cgcgacgacg
      721 atgcattgcg agccgaactt gtcggccgcc tcggcgacga actcgggatt gaagaccgcc
      781 gcggtattga tgctgacctt gtcggcgccg gcattgagca tgcggcgcac gtcggcgagt
      841 tcgcgaatac cgccgcccac cgtcagcgga atgaacactt cgcgggccac cgcttcgacc
      901 acgtgtgcca tggtctcgcg atcgtcggaa ctggcggtga tgtcgagaaa ggtcagttcg
      961 tccgcgccct cgcgattgta gcggcgcgcg acctccaccg ggtcaccggc atcgcgaata
     1021 tcgacaaagc gcaccccctt gacgacgcgg ccagcgtcga cgtcgagaca gggaatgata
     1081 cgtttggcga gcgacatggt tgaggcttcg tcgaactaat gagagtccac gtaggcctgg
     1141 gcctcgacca ggtcgatggt gcgttcgtaa agcgcacgac cgatgatcac cccctcgatg
     1201 ccggacaact tgaccgcgtt gagcttgacg atgcaatcca tgtcggtaac cccgcccgag
     1261 gcgatcaccg gaaccgagat cgccgcggcc agttttttcg tcgcctcgac attgactccc
     1321 tgcatcatgc cgtcacggct gatatcggtg tagacgatcg ccgacactcc ctggtcctcg
     1381 aagcgcagcg cgaactccag cgcactgtgc tcggactcct cttcccagcc atgggttgca
     1441 accatgccgt tgcgcgcgtc gagaccgatg atgacctgcc ccggaaactc cgaacacagc
     1501 tgggtgatga attccgggcg cttgacggcc atggtgccga tgatgacgaa atccaccccg
     1561 gcctcgaggt aggcctcgac ggtatcgatc gagcgaatgc cgccgccgat ctggattacg
     1621 agattcggaa acgaggcaca gatttcctcg atcacgtcag cattgatcgg caggccctcg
     1681 aaggcgccgt ccagatcgac caggtgcagc cgcttgcagc cctgggttac ccacttgccg
     1741 gccatcgcca ccggatcgtc ggaaaatacc gtggtctctt ccatgttgcc ctgtcgcagg
     1801 cgcacgcagt ggccttcctt gagatcgatt gcaggtatca gaatcatgat tgtccgttcc
     1861 aggcgacgaa attgcgcaac agttgcaaac ccgcgtcagc gcttttctcc gggtggcatt
     1921 gcagcgcgaa caggttatcg cgcgcgatcg cgctggtaaa cgcgaagccg taatcggttt
     1981 gcgcggcgac cagttcgcca tcgggctcgt cgagatagta gctgtgcacg aagtaaaacc
     2041 ggctgccatc gtttatcgac cgccacaaag gatgctcgcg ggttcgccta acgcgattcc
     2101 agcccatgtg cggcaccttg aggcggacgc cgtccgcgtc acgatgatca tcgccgaaat
     2161 agcgcactct tcccggcagg taagcaaggc agtcggtgcc gcggttctct tcgctatact
     2221 cgatcaacac ctgcaggccc atgcaaattc cgagaaacgg gcgctgaccg gcggcatcga
     2281 ggatcgcctc gcgcaggccg tagtcgtcga tcgcgcgcat gcagtcaccg gcggcgccct
     2341 gccccggaaa caccacgcga tcggcgccgc gaatctgccg tggatccgaa gtcagcaata
     2401 ccctggcgcg cgcctcgctg accgcctcca gcgcattgac cacggacctc agattgccca
     2461 tgccgtaatc gaccacggca atcgtctgca tggcttacag gcttcccttg gtcgacggca
     2521 ccgcgccggg catgcgctcg tcgcgcgcca cggcaacgcg cagcgcgcgc ccgaaggcct
     2581 tgaagatggt ttcggcgata tggtgggcat tgtcgccggc caggttgtcg atgtgcagcg
     2641 tcgccggggc atggttggcg aatccctgga agaactcgtg gatcaggtcg acgtcgaatt
     2701 gcccgacccg gggacgcctg aagtcgacgc ggtactcgat tcccgggcgc ccggaaaagt
     2761 cgatcaccac gcgcgacagg gcctcgtcga gcggcacgta ggcatggccg tagcgcagga
     2821 tgccctgctt gtcgccgagc gcctgcttga aggcctgacc gagggtgatg ccgatatcct
     2881 cgaccgtatg atggtcgtcg atatgcaggt cgccactggc cttgatatcg agatcgaagg
     2941 cgccgtggcg cgcgatctgc tccagcatgt gctcgagaaa cggaatcccg gtcgcaaagc
     3001 tcgatctacc gctgccgtcc aggttcaacg acacctcgat ctgggtttcc agcgtgttac
     3061 ggctgacagt tgcactacga tctgacattc accaccattc cggcaggctc aaggcttgca
     3121 gataataatc gacatcagcg tcgaattcga cctctaatcg ggagccgcct caatcgagga
     3181 taaaggtcac cggcccgtca ttgatcagct gcactcgcat gtcggcgccg aagcggccgc
     3241 tggcgaccgg ctggtgcacc gcccgtgcgt ggtcgagcgc gagctcgaaa agttgctgcc
     3301 cgcgctgcgg gctcgccgcc gaactgaaac tcgggcgccg ccccttgcgg gtatccgccg
     3361 ccagcgtgaa ttgcggcacc agcagcaggc cgccctcgac atcccgcagc gacaggttca
     3421 tcttgccgtc ggcgtcggcg aacacgcggt aattcaacaa gcgctcgata gcggccctgg
     3481 cgctggcctc gtcatcctgc gcgcggacgc cggcgagcac cagtaatccc cggccgatgg
     3541 cgccgaccac ctgaccatcg acctcgacac gggcctcgct gacacgctgc aacagcgtga
     3601 tcaagcaacc acttccagca tcgcgttgca ggattccacc agcgccttgc tgattccctg
     3661 ctcggtgacc gcgtgcccgg cgtcgctgac gatacgcagc tcggcttgct gccagcgttc
     3721 cttgagcagc agcgcctggt cgaccgggca caccatgtcg tagcgcccgt ggacgatgaa
     3781 accgggaata tgcgcgatgc gttccatgtc ctccagcaac tgattgggac gcaggaaaca
     3841 gtgattcgtg aagtagtggc attcgatgcg cgcaatgctc agcgcgatgt ggggatcggc
     3901 gaaatgctcg accacgcttt tgtccggcag cagggtcacc gaggacccct cccagatcga
     3961 ccaggccttg gcggcgccca tgcgcacgat ttcgttcttg ctggtgagcc gccggtaata
     4021 ggcaccgatg atgtcgtcgc gttcctcggg tggaatcggc tcgatgaaat gttcccaggc
     4081 ttccgggtag acgcgggccg cgtggccgcc gaaaaaccag tcgacgtcgc gctcgcgcgc
     4141 gaggaagata ccgcgcagga tcaggcccat gacccgctcg ggatgcgtct gcgcataggc
     4201 cagcgccagc gtcgaccccc acgaaccgcc aaacacgacc cagcgctcga ttttcaggaa
     4261 ctcgcgaatc ttttcgagat ccgcgatcag ctccggagtg gtattgtttt cgaggctcgc
     4321 gtgcggacgc gacttgccgg caccgcgctg gtcgaaaaga atgatgcgat acgactccgg
     4381 atcgaagaag cgccggtggc cgggttcaca ggacccgccg ggcccgccgt gcaggaaaac
     4441 gatcggcacg ccgttcggat tgccgcactc ttccaggtac agggtgtgca ggtcggaaac
     4501 cggaaagcgc caggtctggc cgggttgaat ggccggaaat aacatgcgta ttgctatacc
     4561 ttcttttctt tatattgtag gacagctcct acaaaactga atggggcaag ctgacaaccc
     4621 ctgagcccgg acgcgctatc ggcgcagcga ggaacgtggg aatatactag cacgacacag
     4681 ggactggtcg acaggtaagg atgccgaatc gtgcagggac gcacacctga attactcatg
     4741 aaatccgtca gttaatctct ctcctcgagc tcggcagctt gccctggtgc ttcgcaccag
     4801 ggatctttgt gtgctcaacg ccatccgtgc tcgccctgcg ctcagagatt gccaaaatcg
     4861 tatagctcgc gatcgagcag gtgcgacggc gtgacccggc acaacgacct gaggatattg
     4921 tcgacccgcc cgggatggct gagatcccac tcacgcagca tcgccttgat cgcctggcgc
     4981 tgcagattgg gttgcgagcc gcacaggttg cagggaatga tcgggaatcg gcgcagcgcc
     5041 gcatactgct tgatcaagac ttcgcgcagg taggcgagcg gccgaatcac cacatggcgg
     5101 ccatcgtcgc tgcgcagttt ggccggcatc gccttcagcc ggctgttgaa aaacatgttc
     5161 agaaaggcgg tctcgacgat gtcgtcggca tggtggccaa gcgcgatccg cgtggcaccg
     5221 atttcactgg cggtgttgta gagcacgccg cggcgcagcc tcgagcacag tgaacaggta
     5281 gtcttgccct cgggaatcac gcgcttgacg acgctatagg tatcctcttc gacgatgcgg
     5341 tattcgacgc cgagcgattc caggtattcg ggcagcacac gctcgggaaa gcctggctgc
     5401 ttctggtcga gattgacggc caccagttcg aaatcgatcg gcgccgcctt ttgcaggccg
     5461 agcagcacgt cgagcagcgt gtaactgtcg gcaccacccg acaggcagac catgacccgg
     5521 tcgccgtccg cgatcatttc gaattcggcc accgcacgcc cggcgccgtg gcgaatccgc
     5581 cgctgcagtg attccagatc tcgcgacagt ccgctgctgt ctatctccat cgggttggcg
     5641 ttcacggtac tattttctcc agaatgccgg cgagaacagc accagcagcg tgaagatttc
     5701 caggcgtccg agcagcatcg ccacgcacag tatccacttg gccgggtcgt tgatcgaggc
     5761 gaaattgcgg ccgacctcgc cgaggcccgg accgaggttg ttgaggcagg cggcgaccgc
     5821 cgaaaacgcg gtcacctggt cgagcccggt cgccatcagc agcaggatca gcaacgccga
     5881 aatcgcgaaa taggcggcga aaaagcccca taccgcctcg atgacctttt ccggcatcgc
     5941 cttcttgccg atcttgaccg cgaactgcgc attcgggtgc accagccggc gcaactcgcg
     6001 cacgccctgc ttgaccagca acaggatgcg aatcaccttg atgccgccac cggtcgagcc
     6061 cgagcagccg ccgataaagc tgacgtagag caacagcacc ggcaggaacc cgggccaggt
     6121 gtagtactcg gcggtggtgt acccggtagt ggtgccgatc gacaccacct ggaacagccc
     6181 ctcgatgatc gactcggtgg tcgaatcaaa ggtatgcgtg agctgcagat agccaacggt
     6241 gacgatgctg accaagatca gcaccagcat ataggtacga aactcggcgt cacgccggta
     6301 gggttttagc gagcgattgc gaaaagcggt gaaatgaatc gcgaaattga tccccgacaa
     6361 cagcatgaag acgatggcca cgaactggat cgcggtactg tcgaaatggc cgatactcgc
     6421 gtcatgggtc gaaaagccgc cgatcgcaac ggtcgaaaat gagtgcgaca tggcgtcgaa
     6481 gaacgacatg ccggccgccc agtaggcgag caggcaggca accgtcaggc cgagatagat
     6541 ataccacagc gccttggcgg tctcggtgat ccgcggtgtc agcttggtgt ccttgaccgg
     6601 acccggggtt tcggcgcgaa acagctgcat gccgccgacg ccgagcatcg gcagcacggc
     6661 gaccgccagc acgatgatgc ccatgccgcc gagccactgc agctcctggc gatacagcag
     6721 cagcgagtgc ggcagctcgt cgataccgac gattaccgtc gcgccggtgg tggtgatgcc
     6781 ggaaatggac tcgaaaaccg cgtcggtgat cgagatatcc tgtaccccgg acagatagat
     6841 cggcagaccg ccggccaccc ccaacacggt ccagaacagc accacgacca ggaagccgtc
     6901 gcgcaggcgc agctcgttgc ggtgcttgcg aaccggcagg tagagcagga aaccgctcac
     6961 cagcgtcagc gcgaaagcct cgacgaacgg cagcaccgcg ccgtcgccga agatcaaccc
     7021 ggtggcgatc ggcggcagca gcgtgcaact ggagagcagc atcaataaac ccagtatgcg
     7081 cagtatgacc agtggttgca tgcttcgctc gacgcctggt ctaaaagtag gtgatgccga
     7141 cctggaacag cttttcgacc tggccgattc gcttcttgtc ggtcaggaac aggatgacat
     7201 gatcctccga ctcgatcacc gtatcgtggt gcgcgatgat gacttcctca ccgcgcacga
     7261 tcgcgccgat gcgcgcgccc tccggcagct tgatctcctc gattgcgcgg ccgacgaccc
     7321 gcgacgaact gcggtcgccg tgcgcgatgg cctcgatcgc ttccgccgaa ccgcgtctca
     7381 gcgcgtgcac cgcgaccacg tcgccgcgcc tgatgtgcgc cagcagggcg ccgatggtaa
     7441 cctggtgcgg cgagatcgcg atgtcgataa gcccgctttc caccaggtcg acataagccg
     7501 gccggttgat cagcgacatg accttccggg cgcccatgcg cttggccagc atcgacgaca
     7561 ggatattggc ctcgtcgtcg ttggtgagcg cgcagaacac gtccatcgat tcgatgtttt
     7621 cctcctgcag caggtccggg tcggcggcgt cgccgagcag cacgatagcc gagtccagca
     7681 tagccgacag ttcgcgcgcg cgtgccggaa aggcttcgat caccttgacc tggtaagcgt
     7741 tttccatgcg cgcggcaagc tggccgccga tgttgccgcc gccggccagc atgatccgct
     7801 tgttgggctt gtcgagcttg cgcatttccg aaatgacctt cgggatatgc tcgcgcgcgg
     7861 cgaagaaaaa cacttcgtcg ccggcctcga tgacacattc cgcggtgggt tgaatcggct
     7921 gcccgtcgcg aaagatcgcc gccacgcgca tgtcgacacc tttcagatgc tcggtggtgg
     7981 tgcgcaactc gtgtccgacc agcagaccgt cgtcgtaggc cttgaccgcg accagctgcg
     8041 cgcggccctt ggcgaaatcg agcacctgca gcgcgctcgg atgctcgatc aggtggtaga
     8101 tgtagtcggt caccagcgac tccgggctga tcatcacgtc gaccggtatc gattcctcgg
     8161 caaacaaacc cggccgcccg aggtactcgg gtgagcgaat gcgcgcgatc ttggtcggcg
     8221 tatgaaacag cgaagacgcg atctggcagg cgatcatgtt gatctcgtcg ctgttggtca
     8281 gtgcgatcag catgtcggca tcgtcggcgc cggcatcttc gagcagcccc gggtaggaac
     8341 cgttgccgca gatggtgcgc aaatcgagcc ggtcctgcag gtccgacagg atctgctggt
     8401 tggtatcgat aacggtgatc tcgttactct cttccttggc gagttcggtt gctacggtcg
     8461 agcctacctg cccggcgcct aagatgatta tattcatggc ctgttattgt tttttggtct
     8521 gcagtcccag ggatttcagc ttgcggtaca gatgggtacg ctcctggccg acacgacgcg
     8581 cgagttcggt catattattg ccgctggcgg ccaactggcg cgacaggtac tcgcgttcga
     8641 aggcttcccg cgcctcgcgt aattcaaggt tcatatcgat ggtgatctgc atcttgtcgc
     8701 cggtttcggt ttcgcgttcg ccctcggtgt cgaattgtcg caatacccgc cgaatttcgt
     8761 cgagcccgat ttcctcggca tcgccattgg tcagaatcgc ccgaatgaat tgctt
//
LOCUS       DATCZV010000299         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_129557, whole genome shotgun sequence.
ACCESSION   DATCZV010000299 DATCZV010000000
VERSION     DATCZV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129557"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VIW27_06650"
     CDS             complement(<1..158)
                     /locus_tag="VIW27_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5739388.1"
                     /translation="MLKVGFIGLGNVGGKLAGSLLRNGVDLTVRDLDADVAQPFLDGG
                     AKWADSPR"
     gene            334..630
                     /locus_tag="VIW27_06655"
     CDS             334..630
                     /locus_tag="VIW27_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY5739389.1"
                     /translation="MAKLTTVYWRDIPAQVIAKERRETAKIVLTERFAEAIDKAAMRA
                     KMAGTDAYLEQWRREVVNCGRDLQAVVDAAAATLEQAYDDARLDALVSNGGLEP"
     gene            complement(805..1452)
                     /locus_tag="VIW27_06660"
     CDS             complement(805..1452)
                     /locus_tag="VIW27_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY5739390.1"
                     /translation="MCGRFFLDAKAGDILEHYNASPPDLFTARYNIAPSTPVLAFSGA
                     GFDLYRWGLIPSWAKDLTIGNRMFNARAETVAEKPSFRNAYRRRRCLVPASGFYEWRL
                     ENGRKQPYCCHIDHQLFSMAGIWEHWQDAEGNEIQSCAVITTQARGAMAELHQRMPVS
                     IAPRDYADWLDCSSDKTEAADRLLFDSAPDYEYYAVGTMVSNSRNEGPDLIEPLE"
     gene            1525..>2087
                     /locus_tag="VIW27_06665"
     CDS             1525..>2087
                     /locus_tag="VIW27_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5739391.1"
                     /translation="MPLLQAKNLHHSFGDQPLLDNVNLTLEPGERVCLVGRNGSGKST
                     LLKILAGDIRVDEGEIIHAPELRIAELKQEVPQDYTGSVYDCVAQGIGELAGVITEWH
                     HAALASASDAEALSRMQRLQDQIETHNAWNLETRISTTISRLALPADKPFADLSGGLK
                     RRVLLGQALVAEPDLLLLDEPTNHLDI"
BASE COUNT          431 a          657 c          605 g          394 t
ORIGIN      
        1 tcgcgcgggc tatcggccca ctttgcgccg ccatcgagaa acggttgcgc gacatcggca
       61 tcgaggtctc tgaccgttaa atccacgcca tttcgcaaca ggcttccggc gagcttgccg
      121 ccgacgttac cgaggccgat aaaaccaact ttgagcatga gtcccctcca gtccgggttc
      181 gaaactgcgc ggcattctac gccctatcgc gggccgcgga aacaatattt tcaggccccg
      241 gtaatcgatc tacttgatac tggatttgag ccgcgagcgt ctttatactc gcgctgcgtt
      301 gcaatcccgt tttcacccat cgagacccgt ttaatggcca aactgacaac cgtgtactgg
      361 cgcgatatcc ccgcccaggt aattgccaag gaacgccgcg aaaccgcaaa aatcgtgctt
      421 accgaacgct tcgccgaagc catcgacaag gcagcgatgc gcgccaaaat ggcgggcacc
      481 gacgcctatc tcgagcaatg gcggcgcgag gtcgtcaact gcggccggga tctgcaggcg
      541 gttgtagacg cggccgccgc gacactggag caggcttacg acgacgcgcg cctcgacgcg
      601 ctggtcagca acggcggcct cgagccctga tcctttgaga tgcccgcatt cgcgggcaaa
      661 gtattctggt ctctactatc ggttggctct cgcggaggcg cggaggacgc ggagagcgcg
      721 gagtttctta aaatttccct gcgctctccg cgttccctgc gcctccgcga gaaacaaatc
      781 agggataatt ctattaatct gtaactattc cagcggttcg atcaggtccg ggccttcgtt
      841 gcgtgaatta ctgaccatgg taccgaccgc gtaatactcg tagtccggcg cgctatcgaa
      901 caacaggcga tcggcagcct cggttttatc cgagctgcag tccagccagt ctgcgtaatc
      961 ccgcggggcg atggatacag gcatgcgctg atgcagctcc gccatcgcgc cccgcgcctg
     1021 ggtggtgatt accgcgcaag attgaatttc attgccctcg gcatcctgcc agtgctccca
     1081 gattccagcc atcgaaaaca gctgatgatc gatatgacag caatacggtt gcttacggcc
     1141 gttttcgagg cgccattcgt aaaacccgct ggccggcacc aggcagcgcc gacgccggta
     1201 ggcattgcga aacgatggtt tctcggcaac ggtttcggcg cgcgcgttga acatgcggtt
     1261 gccgatggtc aggtctttgg cccaggatgg gatcaggccc cagcggtaga gatcgaatcc
     1321 ggctccgctg aacgccagca ccggcgtgct cggggcgatg ttgtagcgcg ccgtgaacag
     1381 atcgggtggc gacgcgttgt aatgctcgag gatatcgccc gctttggcgt cgagaaaaaa
     1441 acgtccacac atagcgcccc ctgaatcaac tgtaaaattc gcgcgaaagc cgcataatag
     1501 cgcagtttca ttcgcccgat acagatgccg ctactgcagg ccaaaaacct tcaccacagc
     1561 ttcggtgacc agccgctgct cgacaacgtc aacctgacgc tcgagcccgg tgaacgcgtg
     1621 tgcctggtgg gtcgtaacgg cagcggcaaa tcgaccctgc tgaaaatact cgccggcgac
     1681 atccgggtgg acgagggcga gatcatccac gcccccgaac tgcgaatcgc cgagctcaag
     1741 caggaggtgc cgcaggatta taccggcagc gtctacgact gcgtggcgca gggtatcggc
     1801 gagcttgccg gggttattac cgaatggcac catgccgcgc tggcctcggc cagcgatgcc
     1861 gaagcgctaa gccgcatgca gcgcctgcag gaccagatcg aaactcacaa cgcctggaac
     1921 ctcgaaaccc ggatatcgac caccatatcg cggctggcgc tgcccgccga caaacccttc
     1981 gcggatcttt ccggcggcct caagcgccgc gtgctgctgg gccaggcgct ggtggccgag
     2041 cccgacctac tgttgctgga cgagcccacc aaccatctcg atatcga
//
LOCUS       DATCZV010000300         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_447660, whole genome shotgun sequence.
ACCESSION   DATCZV010000300 DATCZV010000000
VERSION     DATCZV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447660"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1374)
                     /gene="nqrF"
                     /locus_tag="VIW27_06670"
     CDS             complement(154..1374)
                     /gene="nqrF"
                     /locus_tag="VIW27_06670"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEY5739392.1"
                     /translation="MREAALGVVFFCIIVVLLTLLVLAARRLLVPRGECDITINERKR
                     VPAAIGDSLLAVCQGAGIQLPSACGGAGTCGLCKLRVTGGGGEAGPQELAHLSAREAA
                     RGGRLACQVRIMGPLSVEVDEAYFEARSWHCRVERAVNVSTLIREIQLRLPDGEMMDF
                     RAGAFVEMTCPPYSLDFEDLEIAAEYRDVWDSMGLCKLRAGSDREVTRAYSMANHPGE
                     AGIVTLNIRLALPPPGRPEVPPGVVSSWLFSLRPGDNVEVRGPYGHFAVEPSERELVF
                     IGGGAGMAPLRAQIVDLLERQDSRRRISYWYGARSRRELYYVELFERLQREHDNFSWH
                     PALSRPQPEDGWEGDTGYIHQVAYDSYLASHPAPESCEYYLCGPPLMVQSVQAMLDEL
                     GVDEDRIHFDDFGS"
     gene            complement(1374..1979)
                     /gene="nqrE"
                     /locus_tag="VIW27_06675"
     CDS             complement(1374..1979)
                     /gene="nqrE"
                     /locus_tag="VIW27_06675"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEY5739393.1"
                     /translation="MSGLGELLLRAVFLDNLALSFFLGMCTFLAISKRVDTAVGVGLA
                     VMFVQTVTVPVNNLIYTWLLAPGALAWAGMGDVDLSFLRLIAFIGVIAAMVQVLEMTL
                     EFHVPRLHRAMGIFLPLITVNCAILGGSLFMVERSYAFVESVVFGFGSGLGWALAIVM
                     FAAIRERLKYADIPDGLQGLGINFIVAGIMSLGFLAFAGMG"
     gene            complement(1976..>2259)
                     /locus_tag="VIW27_06680"
     CDS             complement(1976..>2259)
                     /locus_tag="VIW27_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HEY5739394.1"
                     /translation="SVLDGIGNGLGYSLILVTVGSIREFFGKGSLLGVQLVDTVEQGG
                     VFEPLHLMLLPPSAFFIIGGIIWLIRRQRPQQVEKAEFTLRPRAEEGAE"
BASE COUNT          458 a          779 c          686 g          336 t
ORIGIN      
        1 accccttcgc gtcaggcagg tgctgcaatt gccacaactg ccgcaaggcg gcttgcgccc
       61 gaggatcaga ccgaggccga gcccggccac cgccaaaacg aacagcggca ccgcaatcaa
      121 tatcgtcgtc atcgaacaag cctccccggc agatcaactg ccgaaatcgt caaagtgaat
      181 ccggtcctcg tcgaccccga gttcgtcgag catcgcctgg accgactgca ccatcagcgg
      241 cggaccgcac agataatact cgcagctttc gggggccggg tgactcgcca ggtagctgtc
      301 gtaggccacc tggtgaatat acccggtatc cccttcccag ccatcctcgg gctgcggccg
      361 cgacagcgcc ggatgccagc tgaagttgtc gtgctcgcgc tgcaggcgtt cgaacagttc
      421 gacataatac agctcgcgcc gcgaacgtgc gccgtaccag tagctgatcc ggcgtcgcga
      481 atcctgccgt tcgagcaggt cgacgatttg tgcacgcagc ggggccatgc cggcgccgcc
      541 gccgatgaat accagttccc gctccgaggg ctcgaccgcg aaatggccgt aaggccccct
      601 gacttcgaca ttgtcgcccg gccgcagcga gaacagccag ctggaaacca cgcccggagg
      661 gacttcggga cgtcccggcg gcggcagcgc caggcgaata ttcagcgtca cgatgcccgc
      721 ctcgccggga tggttggcca tcgagtaggc gcgcgtcact tcccggtcgc tgccggcacg
      781 cagcttacac agtcccatgc tatcccagac gtcgcggtat tccgctgcga tctcgagatc
      841 ctcgaaatcc agcgagtaag gcgggcaggt catttcgacg aaggcgccgg cgcgaaaatc
      901 catcatttcg ccgtcgggca gtcgcagttg aatttcacgg atcagcgtgc tgacgttgac
      961 ggcccgctcc acccggcaat gccagcttct ggcctcgaaa tacgcctcgt cgacctccac
     1021 cgacagcggc cccatgatcc gtacctgaca ggccaggcga ccgcctctcg cggcctcgcg
     1081 cgcgctcagg tgggcgagct cctgcggccc ggcctcgccg ccaccaccgg tgacccgcag
     1141 cttgcacagg ccgcaggtac cggcgccgcc gcaggcgctc ggcaactgga tgccggcacc
     1201 ctggcagacc gcgagcaggc tgtcgccgat agcggctggc acgcgcttgc gctcgttgat
     1261 ggtgatgtcg cactcgccgc gcggtaccag caggcgccgc gcggccagca ccagcagcgt
     1321 cagcagcacg acgatgatgc agaagaagac gaccccgaga gcggcttcac gcatcagccc
     1381 atacctgcaa aggcgagaaa accgagcgac atgattccgg cgacgatgaa gttgattccc
     1441 aggccctgca gcccgtcggg tatgtcggca tatttcaagc gctcgcgaat cgcggcgaac
     1501 atgacgatcg ccagtgccca gccgaggccg ctaccgaaac cgaaaaccac gctctcgaca
     1561 aaggcgtagc tgcgctcgac catgaacagg ctgccaccga gaatcgcgca gttgaccgtg
     1621 atcagcggca ggaaaatacc catcgcacga tgcagtcgcg gcacgtgaaa ttcgagcgtc
     1681 atttccagca cctgcaccat cgccgcgatc acgccgatga acgcgatcag gcgcaggaac
     1741 gacaggtcga catcgcccat gccggcccag gccagcgcgc cgggcgccag cagccaggtg
     1801 tagatcaggt tgttgacggg caccgtcacc gtctgcacga acatcaccgc gagcccgacg
     1861 ccgaccgcgg tgtcgacgcg tttcgaaatg gcgagaaagg tgcacatgcc gagaaagaag
     1921 gacagcgcca ggttgtcgag aaacaccgcg cgcagcagca gttcgccgag tccgctcatt
     1981 cggcgccctc ctcggcccgc gggcgcagcg tgaattccgc cttctcgacc tgctgcgggc
     2041 gctggcgacg gatcaaccag atgatgccgc cgatgatgaa gaaggcactc ggcggcagca
     2101 gcatcaggtg cagcggttcg aacacgccac cctgctcgac ggtatccacg agctgcacgc
     2161 cgagcaggct gcccttgccg aaaaactcgc ggatggatcc caccgtgacc agaatcagcg
     2221 aataaccgag gccgttgccg atgccgtcga gcacgctgt
//
LOCUS       DATCZV010000301         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_76583, whole genome shotgun sequence.
ACCESSION   DATCZV010000301 DATCZV010000000
VERSION     DATCZV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76583"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1090)
                     /locus_tag="VIW27_06685"
     CDS             complement(251..1090)
                     /locus_tag="VIW27_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5739395.1"
                     /translation="MNDLDQRSVLITGCSSGIGECVAHGLHRRGYRVFASVRNRDDCE
                     PYRQAGIECLVLDYADSASIEAAVAQLLKRTGGRLYALFNNGAYGQPGACEDLSRAAL
                     REQFETNVFGWMELTNRIIPVMRAQGYGRIIHNSSILGFAAMPMRGAYNASKFAIEGF
                     SDTMRLELADTDIHVSLIEPGPIESRFRANAYRAFQRHIEVENSVHRQRYQKMIERFE
                     TSGNVQPFTLPPEAVLKRVVHALESRRPKIRYPVTVPTYLFAWLKRVLSSRMLDRLLE
                     RSG"
     gene            complement(1103..1933)
                     /locus_tag="VIW27_06690"
     CDS             complement(1103..1933)
                     /locus_tag="VIW27_06690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5739396.1"
                     /translation="MELIVFDLDGTLLNRQSTISDYTSETLRLLSQREIAYTVATGRT
                     LHGARAILEGHRFDLPQVFKNGVMIWHPQQRCFSSSNTLTAAELENVVHACIHQRVTP
                     FVFTLDEDDNSTVYHGALRTAADFELVRVLGLDGHLRMRALDELPSNAAVTHINSIGA
                     PAAIRAIVDSVEDQAHLVAYSGLAREGADWHWLDVHHSDASKGGAIQTLKALLGLERV
                     VCFGDSDNDLSMFAAADEAYAPANANDAVKTAATAVIGHHDEEGIARFLRARFALDES
                     "
     gene            2235..>2615
                     /locus_tag="VIW27_06695"
     CDS             2235..>2615
                     /locus_tag="VIW27_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY5739397.1"
                     /translation="MKTSTRSASRLPTLAALPSEPHLVDIGRPADTIEAFLQSTTLIV
                     NITSKDIDGFQWLLGKIEDSAIESVLFVSSTSVYPPDSGIVSEADGVESPHHPLVVIE
                     NLFRQSREFRTTVIRFGGLIGPGRH"
BASE COUNT          444 a          788 c          884 g          499 t
ORIGIN      
        1 cgtcatgccc gcgaaagcgg gcatcctgca atggtggcgt gatctggaga ttcccgcttg
       61 cgcgggaatg cccccgggtt gcgggaatga cttcgggttg gcggaacgac tcaggtatcg
      121 tcatgcccgc gaaagcgggc atcctgcaat ggtggcgtga tctggagatt cccgcttgcg
      181 cgggaatgcc cccgggttgc gggaatgact caagattgcg ggaatgtcgg ggttttcggg
      241 gagcggcggg tcagccggag cgctcgagca gccggtccag catgcgactg ctgagcacgc
      301 gcttcagcca cgcgaacagg taggtcggca cggttaccgg gtagcggatt ttgggtcgcc
      361 tgctctccag cgcgtgcacc acgcgcttta acacggcctc cggcggcagc gtaaagggtt
      421 gcacgttacc gctggtctcg aaacgctcga tcatcttttg gtagcgctgg cgatgcacgc
      481 tgttttcgac ctcgatgtgt cgctggaacg cgcggtaggc gttggctcga aagcggctct
      541 cgatcggccc gggctcgatc agactgacgt gaatatcggt gtcggcgagc tccagtctca
      601 tcgtgtcgct gaagccctcg atggcgaact tgctggcgtt gtaggcgccg cgcatcggca
      661 tcgcggcaaa gcccaggatc gagctgttgt ggatgatacg gccgtagcct tgcgcgcgca
      721 tcaccgggat gatgcgattg gtcagctcca tccagccgaa tacgttggtt tcgaattgct
      781 cgcgcagcgc cgcgcggctc agatcttcgc aggcgccggg ctggccgtag gcgccattgt
      841 tgaacagcgc gtacaaacgc ccgccggtcc gcttaagtag ctgcgcgacg gcagcttcga
      901 tactcgcgga atccgcgtaa tcgagtacca ggcactcgat gccggcctgg cgatagggtt
      961 cgcagtcgtc gcgattgcgg acgctggcga agacccggta accgcgccgg tgcaagccgt
     1021 gggccacgca ttcgccgata ccgctggagc agccggtgat cagcacgctg cgttggtcga
     1081 ggtcgttcat cggcgccagc gctcaggatt catcgagggc aaatcgcgcg cgcaggaaac
     1141 gggcaatgcc ttcttcgtcg tgatgcccga tcaccgcggt ggcggcggtt ttcacggcat
     1201 cgttggcatt ggccggtgcg taggcctcgt cggcggccgc gaacatgctg agatcgttgt
     1261 cgctgtcgcc gaaacagacg acgcgctcga ggcccagtaa tgccttcagc gtctgaatcg
     1321 cgccgccctt gctggcgtcg ctgtgatgca cgtccagcca gtgccagtcg gcgccttcgc
     1381 gagcgaggcc ggaataggcg accaggtgcg cctggtcctc gacgctgtcg acgatcgccc
     1441 gaatcgcggc gggtgcgccg atcgagttga tatgcgtgac cgcggcgttc gacggcaatt
     1501 cgtcgagcgc gcgcattcgc aggtggccgt cgaggccgag cacgcgaacc agttcgaaat
     1561 cggcagcggt gcgcagcgcg ccatggtaca cggtgctgtt atcgtcctcg tcgagggtga
     1621 aaacgaaagg cgtcacccgc tggtgaatgc aggcgtgcac gacgttttcg agctcggcgg
     1681 cggtcagcgt gttgctgctc gaaaagcacc gctgctgtgg atgccagatc atcacgccgt
     1741 tcttgaaaac ctgcggcaga tcgaagcgat ggccttcgag gatcgcgcgc gcgccgtgca
     1801 gcgtgcggcc ggtggccacg gtataggcga tctcgcgctg cgacagcagt cgcagcgtct
     1861 cgctggtgta atccgagatc gtcgattgcc ggttgagcag cgttccgtcg aggtcgaaaa
     1921 cgatcagttc catcggtacc aggtcagcgc aacgagtggg cgatagtgtc gccgttaggc
     1981 cgcttcgatg caaacccgat tatcggcgta gcggcacgca ctcggtatgc cgatcgacga
     2041 acggcgggcg acccggcagg cgtggaattt gccgcccttt gaaaggcgag cggtccgctg
     2101 gccgccggca aacgggtata ctgtgccgct gaacgggcaa ctgcgggagc gggatcatcg
     2161 acagcatcag tattctcggc agcggctggc tcggcctgcc gctcgccagt cgtttcgcgg
     2221 cgcgcggcta ccgggtgaag acgtcgacga ggtcggcctc gcgcctgccg acacttgcgg
     2281 ccttgccgag tgagccccat ctcgtcgata tcggcaggcc cgccgacacc atcgaggcgt
     2341 ttttgcagtc gacgacgttg atcgtcaaca tcacctccaa ggacatcgat ggtttccaat
     2401 ggctgctcgg caagatcgag gattccgcca tcgagtcggt gctgttcgtc agctcgacgt
     2461 cggtgtatcc gccggacagc gggatcgtta gcgaagcgga tggcgtcgaa tcgccgcatc
     2521 acccgttggt cgtcatcgaa aacctgttcc ggcaaagccg cgagtttcgc accaccgtca
     2581 ttcgcttcgg cggactgatc ggtcccgggc gccac
//
LOCUS       DATCZV010000302        15338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_765570, whole genome shotgun sequence.
ACCESSION   DATCZV010000302 DATCZV010000000
VERSION     DATCZV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765570"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VIW27_06700"
     CDS             complement(<1..729)
                     /locus_tag="VIW27_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEY5739398.1"
                     /translation="MSSDAAALAGRSAAPQIKAKVSRDDRIMLGFILVICLYLLITLA
                     FPLYAILSKSFSAYTFNLDNFEFQVNAGQGWSEPVSAAQLNRQTDAYDDVDLATSSDG
                     RLAVVDLFPDFSFRSPTLYRIRQIERESSFLFGSERVADTDWHEYSSNDFRRIVLRPV
                     AALGFENYRTYFSTPSLFRSIENSLWIATISTIITVSLAFGFAYALNRSQMRFKGIFR
                     LVAMIPILVPSLLPGIALVYLFGNQ"
     gene            complement(726..1853)
                     /locus_tag="VIW27_06705"
     CDS             complement(726..1853)
                     /locus_tag="VIW27_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5739399.1"
                     /translation="MNESSSIESASARVYLRIDRLTKKFGSFTALDDVSLEVYEGEFI
                     CFLGPSGCGKTTFLRAIAGLDIQTSGQVFQDGADISALPPSERDFGIVFQSYALFPNL
                     TAWKNIAFGLENQKLDKAKIAARVEELLELVGLPDQGPKYPAQLSGGQQQRIALARAL
                     ATSPGLLLLDEPLSALDAKVRGHLRHEMKQLQRRLGVTTVMVTHDQEEALTMADRIVV
                     MNQGVIEQVGTPTEIYREPDTLFVADFIGAMNQIPGQIAGGSLKLGDLNFTPRQHDIA
                     DGESIVAAIRPEDIVPLGAGGETAGGESGDNRLEVEIEDMEFLGSFWRASLRNDALGE
                     NHLLADMSINVVRHMEVDTGSTIEVELPADRLWVFPADNTV"
     gene            complement(1953..2990)
                     /locus_tag="VIW27_06710"
     CDS             complement(1953..2990)
                     /locus_tag="VIW27_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5739400.1"
                     /translation="MKQKSGAKRVLLTCCAAALWIVGQAAQAATELTVYTAVEAEDLA
                     RYAETFNQDHPDIEIKWVRDSTGIVTAKLLAEKNNPQADVIWGLAATSLLLMKSEGML
                     EPYKPKGVEKLDKKFVDSGAVPTWVGMDAWVASVCYNTVEAAKHNLKPPKTWKDLLDP
                     MYEGHLIMPNPNSSGTGFLDVSSWLQMFGKDKGGWEFMDGLHKNIARYTHSGSKPCKL
                     AAAGEIPIGISFAFRGAKSKAAGAPLEIIVPDEGVGWDMEATAIVAGTSKLKAAQTLV
                     DWSITRKANEMYNTGYAVVAMPGVAKPVEHFPENLLEKMIDNDFEWAANNRKAILAEW
                     LRRYDSKSEAK"
     gene            3286..4338
                     /locus_tag="VIW27_06715"
     CDS             3286..4338
                     /locus_tag="VIW27_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY5739401.1"
                     /translation="MPLMTTCIGAYPKPDYVKLPDWFAGPEGCDTSDPTGAWEQAMAD
                     LGPDAAKIIERGVAEAIADQIACGIDIPTDGEIARENYIHYHCRHLQGFDFANLTRKQ
                     VRGGTYEAALPTISGPVSVRDRFLVDDWKRAQINCRSAVKITMPGPMTISDTNHDGFY
                     NDPKKLGADIADALNVEVRALADAGCIHIQIDEPLFARKPAAALDYGFENLERAFHGC
                     PSRVQRTVHMCCGYPDRLDREDYPKADPDSYLQIADAIEYSSIDAVSFEDAHRHNDLS
                     LLERLPTTRVIFGVVAIARSEVESVEQIRERLLQALQHIDSERLIAAPDCGLGLLGRE
                     LAMAKLTNMCKAADSL"
     gene            complement(4495..5991)
                     /locus_tag="VIW27_06720"
     CDS             complement(4495..5991)
                     /locus_tag="VIW27_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY5739402.1"
                     /translation="MPQANRGEQIMWERLFQLSRGRKVSYQRQLREMIVSAILDDKLP
                     LDLPLPSSRELARHLGVARNTVVLAYQQLIDEGYLVARERSGYYIDKTMLEGRVRVEP
                     LQPDGEHPAPDWQRHIKFHPSRQRNIVKPPDWKKYPYPFLFGQLDPGLFPVADWRECC
                     RQALSVSDIQEITPDRIDADDPLLIEQIQARILPRRGVWAARDEILITMGAQQALYIL
                     ADLLIERGTRVGMENPGYPDARNIFELKAPRMVPLAVDEQGVVPGQSMNACEFVYTTP
                     SHQSPTTVTMPLERRERLLKQAEKHDFLIVEDDYESEINFVGEPTPALKSLDATGRVI
                     YVGSLSKTLAPGLRLGYMVGPEPLIAEARALRRLMVRHPPANNQRIVALFLSMGHHDS
                     LIHRLSKTYKERWQLMGEALNRYLPESARIPSFGGTSYWVEGPAELDTRQLKERARQA
                     GILIEAGDVCFMQDRPPQNFIRLGYSSIAAKKIAPGIEKLAALIHHPA"
     gene            6104..7474
                     /locus_tag="VIW27_06725"
     CDS             6104..7474
                     /locus_tag="VIW27_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5739403.1"
                     /translation="MKPENLNDIVGSDRNHLWHHLMQHKPWQSSDPMVIVEGKGLRVK
                     DANGREYLDAVSGGVWTVNVGYGRESIADAVREQIAKMCYFANSAGNIPGAQFAEKLV
                     DKMPGMSRVYYANSGSEANEKGFKLVRQLAALKNDGKKHKIIYRDRDYHGTTITTLSA
                     GGQAQRKDQYGPFTPGFVEMPHCLAYRDPRGEDPDYGVKAAKDLEKVILAEDPDTVGS
                     VCLEPITAGGGVIVPPEGYWETVQEICSKYGVLIHIDEVVCGMGRTGKWFGYQHFGVK
                     PDIVTMAKGVASGYAAISVMVTTEDLFNEFLAEPSDQLHYFRDISTFGGCAGGPAAAL
                     ENMRIIEQENLLDNVNRMGDYLMDRLYELQDKHAVIGDVRGKGLFCGAELVSDRETRQ
                     PLDESLAAAVVADCLKQGVMIGRTNRSLPQFNNTLCLSPALICTRDDIDEIVAAIDVA
                     LGNLAN"
     gene            7501..8487
                     /locus_tag="VIW27_06730"
     CDS             7501..8487
                     /locus_tag="VIW27_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739404.1"
                     /translation="MIRLLLLIAALSALVPGTVVSAALDKPEKISVGYLPHWPTPVQF
                     ARIKNTFDVALGIEVDWVAFEDGNQMSDALARGDIQIAHSQGHIPFLAAVSRGLDLTM
                     VGVAVDYAEDDNCIVRSDTGIGRGTAALLAGNRVAVRTGSISHFRMLGVLRHLGVDPA
                     QVEIVAVARDGDARAALESGDVVMACASGAVLRELRSLGEPLMNGVGQSAIGLRVFDS
                     ITVASDFVHQHADIVQAFMDIVEASNKQWRQNPQSMLKAIARATDLDLASTRQALAGF
                     NFPTAMEQKSDAWLGGDVVDYSKELADFFVAEGVLPRALESYERFITTRFLR"
     gene            8488..9291
                     /locus_tag="VIW27_06735"
     CDS             8488..9291
                     /locus_tag="VIW27_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEY5739405.1"
                     /translation="MPGHGGGQRLQAESTAREAAALQVFDITERLAAEDFLFIEAART
                     VELLAAISATDIVNDEVFLDSWNRLEEDRYMADGGKYRKRRHAVYAIHNPGETARQMP
                     YQPHYQTLDYNPLNGGVARYFAPILDDLHHSPTLAALLEFGNRVFSRISGNQQWHIEL
                     HQFRIEARDGSLGKPTPEGAHRDGVDFVIVVMIKRVNIDSGATTIFNSEHQLVGEFML
                     RETFDMVLVNDRRVYHGVTPITQLDAGLEAYRDVLVVTFKKPDNSQAGA"
     gene            9477..11123
                     /locus_tag="VIW27_06740"
     CDS             9477..11123
                     /locus_tag="VIW27_06740"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY5739406.1"
                     /translation="MEREAMNFDVVIVGAGPAGLTMGIRLAQLAREQERELSICILEK
                     GAEVGAHILSGAVIEPRALDELLPDWKERGAPLNVPVKADQFKYLTAKGAISMPTPPQ
                     MDNEGNYIISLGNFCRWLAEQAEELGVEIFPGFAAAEVLYDEAGAVRGVATGDMGIGK
                     DGEPTDGYEPGVELIGRQTVFAEGCRGSLSGELMQKFGLRDGVEPQTYGIGIKELWEV
                     RPEVHAEGTALHTIGWPLQSDTYGGSFLYHNENNQVAVGFVIGLDYRNPHLSPFEEFQ
                     RFKTHPAIRPLFEGGKRIAYGARAISEGGLQSLPKLTFPGGLLVGDTAGFLNVPKIKG
                     THTAMKSAMLAAEAVFESLPADYDEENPGPVLEATAYADKFRQSWLYRELNQVRNIRP
                     GFHRGLWWGLVLAALESYLFRGKAPWTFGHHADHEQLGHKNDYPKIDYPKPDGEVSFD
                     RNSSVYLSGTYHEENQPAHLRLRDDSVPITLNLEKYDAPEQRYCPAGVYEIVQRDGQP
                     ALQINAQNCVHCKTCDIKDPSQNIHWVVPEGGGGPAYPNR"
     gene            11333..11680
                     /locus_tag="VIW27_06745"
     CDS             11333..11680
                     /locus_tag="VIW27_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HEY5739407.1"
                     /translation="MPHLIIEYSEGVDGTVAIPDIVDAAHAGAMSSELFPEYDIKTRA
                     IGYRDHRTGATRDSFVHVTVRLLDGRSDAQKAMLSEAVLAHIEPLLPGVNSVGVEIVD
                     MHRASYRKRVLDG"
     gene            complement(11688..12710)
                     /locus_tag="VIW27_06750"
     CDS             complement(11688..12710)
                     /locus_tag="VIW27_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5739408.1"
                     /translation="MTDNCLLPEFELDEAADIARRLFAIDGPIRRLDGERDLNFLIGA
                     PGDRFVFKIANAEESPAMLECQHQVFERLAAAGVFPATVTARQSVNGKTIESVRDANG
                     HAHVCRVLPYVEGRLFREIENPSAQLLADIGCRLALLDRALDGFSHAALERPLLWKMD
                     TALEIVDGYSPLLDPESRALADHFADGYRRRVVPRIAELRRTVIHNDANRNNLVVDES
                     AAHLLSIIDFGDMLEGWLAVDPSIAATYVMLDRAQPLEAAAALLAGYCERMPLQPAEA
                     ETVFDFICMRLCMSLCIGAHQCAQQPDNPYLGTDLDAVRQLLAELRHLEPAAATAALF
                     AGRQRD"
     gene            12951..14981
                     /locus_tag="VIW27_06755"
     CDS             12951..14981
                     /locus_tag="VIW27_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019811959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY5739409.1"
                     /translation="MALRLGIDTGGTFTDAVLVDDDKRIVNAEKCLTTRFDLSIGIGD
                     VVARLPAASLAEVIMVSLSTTLTTNSVVEDLGAPVCVLLPGYNPEQIKQSGLLNILPA
                     QFVVRLQGGFDAVGREHAPLDEAGAREAIERLQDEVSAFAISSMFGTRNSSHENRLKQ
                     MVAEITGMPVICGHELASSLGAPRRALTAALNARMVPPIKELIGSVQKILDRHRIDAP
                     LMLVKGDGSLASLENAIEKPIGTVLSGPAASVIGACQLSGLENAIVADMGGTTTDIAI
                     VSNGQPQLSEEGANIGDWQPMVEAVRVNSIGLGGDSEIRFGGHTGLSVGPRRVVPTSL
                     LGHLYPEIIPRLEAQLAGMTSARSNRFVMRLESNPALLRQLGKIEREAWELLSDRPVE
                     LDAIVAENRAYARALARLKRMGLAIYSGFTPTDAVHVLGINSHWSKPAAELSARLWMR
                     QMRFLYGYGKWDSDDIETACREVYELVNASIRRLLIEVGLNEFSLVGKTGKSGAVVDT
                     ITRMVLGSHQHQGRKPLFNLDFASDYPIVAVGAPAASYYRDVADSMGIELYLPEYAEV
                     ANAFGAVMGSVIQRAQVTVTQPQHGIFCLFHEDQPKNFDTLEAAKQAAQEIVSRLAQQ
                     RALAAGAIEPTVTLAFEELHVKDKVDGELFLESTVIATAIGRACDWRTERSA"
     gene            14978..>15338
                     /locus_tag="VIW27_06760"
     CDS             14978..>15338
                     /locus_tag="VIW27_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5739410.1"
                     /translation="MNLDRRLRDHGLFVRGVTRLADDEIDAYGLAADRPWVALVGNIG
                     SSYWSAFSAADEYRDGAADPLDRWSRRIAETLASEFALQAIFPFEGPPYYPFQRWARR
                     AEGLAQSPLGIMIHPEHG"
BASE COUNT         2912 a         4693 c         4862 g         2871 t
ORIGIN      
        1 ctggttgccg aacaggtaga ccagtgcgat gccgggcagc agcgatggca ccaggatcgg
       61 gatcattgcc accagcctga agatgccctt gaagcgcatc tggctgcggt tcagcgcata
      121 ggcgaagccg aacgccaggc tgaccgtaat gatcgtgctg atggttgcga tccacagcga
      181 attctcgatc gaacgaaaca gcgaaggcgt ggaaaaatag gtgcgatagt tttcgaagcc
      241 gagcgcggcc accgggcgca gcacgatacg gcggaaatcg ttggagctgt attcgtgcca
      301 gtcggtgtcg gcgacgcgtt cgctgccgaa caggaacgag ctttcccttt cgatctggcg
      361 aatgcggtac agcgtcgggc tgcggaagct gaaatccggg aataagtcga ccactgccag
      421 gcggccgtcc gagctggtgg cgaggtcgac gtcgtcataa gcgtcggttt gccgattcag
      481 ttgcgccgca ctgaccggct cgctccagcc ttgcccggcg ttgacctgaa actcgaagtt
      541 gtcgagattg aaggtatagg cggagaagga cttcgacaaa atcgcataca gcgggaacgc
      601 cagcgtgatc agcaggtaca ggcagatgac caggatgaag ccgagcatga tgcggtcgtc
      661 gcggctgacc ttcgccttga tctgcggtgc cgcggaacgt ccggcaagtg ctgccgcgtc
      721 gctactcata ccgtattatc ggcaggaaaa acccacaggc ggtcggcggg cagttcgact
      781 tcgatggtcg aacccgtgtc gacttccatg tgtcgcacga cgtttatcga catgtcggcc
      841 aacaggtgat tttcgccgag cgcatcgtta cgcagggatg cccgccagaa ggagccgaga
      901 aactccatgt cctcgatttc gacctcgagg cggttgtcgc cactctcgcc gccggcggtt
      961 tcgccgccag cgccgagcgg aacgatatcc tcgggccgaa tcgcggcgac gatcgattcg
     1021 ccgtcggcga tatcgtgctg gcgtggcgtg aagttcaggt cgccgagttt caggctgccg
     1081 ccggcgatct gcccgggaat ctggttcatc gcgccgatga agtcggcgac gaacagcgta
     1141 tcgggttcgc gatagatttc ggtcggcgtg ccgacctgct cgattacgcc ctggttcatg
     1201 acgactatcc ggtcagccat cgtcagggcc tcctcctggt cgtgggttac catcacggta
     1261 gtgacgccca ggcggcgttg cagctgtttc atttcatggc gtagatgccc gcgcaccttg
     1321 gcgtcgagcg ccgagagcgg ctcgtcgagc aacaacaggc caggcgaagt cgccagcgca
     1381 cgggccagtg cgatgcgctg ctgttgcccg ccggacagct gcgcggggta cttgggtccc
     1441 tggtccggca ggccaaccag ttccagcaat tcctcgacgc gggcggcgat tttcgccttg
     1501 tcgagtttct gattctcgag gccgaaggcg atgtttttcc acgcagtcag gttcgggaac
     1561 agtgcgtagg actggaacac gatgccgaag tcgcgctccg aggggggcag cgcggagata
     1621 tcggcaccat cctggaatac ctggcccgag gtctggatgt cgagcccggc gatggcgcgc
     1681 agaaaagtgg tcttgccgca gcccgaaggg ccgaggaaac aaatgaactc gccctcgtac
     1741 acttccagcg aaacgtcgtc aagcgcggta aacgacccga acttcttggt cagtcggtct
     1801 atccgcaggt aaaccctggc ggacgcggat tcaatcgaag acgattcgtt catggtctgt
     1861 gataacagcg ggtcggtgcg cggtgccgaa tctaagccgc ggtgagaccc ggccctgcga
     1921 gagggccggg tgatcatacg gcttgccgaa aactacttgg cctcagactt ggaatcgtaa
     1981 cgcctgagcc actcggccag gatcgccttg cggttgttgg ctgcccactc gaagtcgttg
     2041 tcgatcattt tttccagcag gttttccgga aagtgctcga ccggcttggc gacgccgggc
     2101 atcgcgacca ccgcgtaacc ggtgttgtac atttcattgg ccttgcgcgt aatcgaccag
     2161 tcgaccagcg tttgcgcggc tttcagcttt gaagtgccgg cgacgatcgc ggtcgcttcc
     2221 atgtcccagc cgacgccctc gtcgggcaca atgatctcca gcggagcgcc tgcggccttt
     2281 gacttggcgc cgcggaacgc gaatgaaata ccgatcggga tttcgccggc cgcggccagc
     2341 ttgcacggct tggatccgga gtgggtgtag cgcgcgatat tcttgtgcag gccgtccatg
     2401 aactcccagc cgcccttgtc cttgccgaac atttgcagcc agctggaaac atcgaggaag
     2461 ccggtgcccg acgaattcgg gttcggcatg atcaggtgac cctcgtacat cgggtcgagc
     2521 aggtccttcc aggtcttcgg cggcttgagg ttatgcttgg ccgcttcgac ggtgttgtag
     2581 caaaccgagg ccacccacgc gtccatgccg acccaggtcg ggacggcgcc gctgtcgacg
     2641 aatttcttgt ccagcttctc gacgcccttc ggtttgtagg gctcgagcat gccctcgctc
     2701 ttcatcaata gcagcgaggt cgccgcaagg ccccagataa cgtccgcctg cgggttgttt
     2761 ttctcggcca gcaacttggc ggtgacgata ccggtggagt cgcgaaccca cttgatctcg
     2821 atatcggggt gatcctgatt gaaagtttcg gcgtaccgcg cgaggtcttc tgcttcgacc
     2881 gcggtataaa cggtcagctc ggtcgccgcc tgtgcggcct ggccgacgat ccacagtgcc
     2941 gcggcacagc aggtgagtaa tacgcgcttg gcgcctgatt tctgcttcat tacagtctcc
     3001 tctggttgtt ttcgggtgtt atgcccaatt ttctgttgat tgttattacc gggtatgcgt
     3061 gtcggcctgg tgtttgccgc gcgcgtaacc ggaaagcgat actctaccct aaatgacacg
     3121 ggttaaaaat aagttaaaat cgatacgaaa tgccagttct tccggtaggg gccccgatga
     3181 gggcttttgc gataccgaat cgagttgcta gtattcgcga attccgcact cagagttacc
     3241 gaatgcgggg cttgtaacag cgataattga aacagggtaa aacaaatgcc gttgatgacc
     3301 acctgtatcg gagcttaccc caagcccgac tacgtcaagt tgcccgactg gtttgccggc
     3361 cccgagggtt gcgacacctc tgatccgacc ggggcctggg agcaggcgat ggccgacctc
     3421 gggccagatg cggcgaagat catcgagcgc ggcgtggccg aggctattgc cgaccagatc
     3481 gcctgcggta tcgacattcc gaccgatggc gaaatcgcgc gcgagaatta cattcactac
     3541 cattgccggc acctgcaggg attcgacttc gccaacctga cgcgcaagca ggtgcgtggc
     3601 ggcacttacg aggccgcctt gccgacgatt agcgggccgg tgtcggtgcg cgatcggttt
     3661 ctggtcgacg actggaaacg cgcgcagatc aattgccgca gcgccgtcaa gatcacgatg
     3721 ccggggccga tgacgatttc cgataccaat cacgacggtt tttataacga tccgaaaaag
     3781 ctcggtgcgg acatcgccga tgcgctcaac gtcgaggtac gtgcgctggc cgatgccggc
     3841 tgcattcaca tccagatcga cgagccgctg ttcgcgcgca agcccgctgc cgcgctcgac
     3901 tatggcttcg aaaacctcga gcgcgcgttt cacggctgcc cgagccgcgt gcagcgcacc
     3961 gtgcacatgt gctgcggata tcccgaccgc ctcgatcgcg aggattaccc gaaggccgat
     4021 ccggactcct acctgcagat cgccgatgcg atcgagtact cgagtatcga cgcggtgtcg
     4081 ttcgaggacg cgcatcgcca caacgacctg tcgttgctgg agcgcctgcc gactaccagg
     4141 gtgattttcg gcgtcgtcgc gatcgcgcgc agcgaggtcg aaagtgttga gcagattcgc
     4201 gagcgcctgc tgcaggcgtt gcagcacatc gactccgaac ggctgatcgc cgcgccggat
     4261 tgcggactcg gcctgctcgg acgcgaactc gcgatggcca agctcaccaa catgtgcaag
     4321 gccgccgact ccctctaaat tggggtcaga gtcaattaaa ttggggtcag agtgagcttc
     4381 ccccaaaatt aaattggggt cagagtcaat taatttagca accagccgaa atctgactct
     4441 gaccccaatt tataccggca actcggaaaa ttgactctga ccccaattta gggatcaggc
     4501 ggggtggtgg atcagtgcgg cgagtttttc tatgccgggg gcgatttttt tggccgcgat
     4561 cgacgagtag ccgaggcgaa tgaagttttg cggcggacgg tcctgcatga agcagacgtc
     4621 accggcctcg atcaggatgc cggcttgacg cgcacgctcc ttcagttgtc gagtatcgag
     4681 ttcggccggg ccctcgaccc agtaagaggt gccgccgaac gaaggtatac gcgccgattc
     4741 cggcaggtaa cgattcagcg cctcgcccat cagttgccag cgttccttgt aggttttgct
     4801 gagccggtgg atcaacgaat cgtggtggcc catcgacagg aacaaagcga cgatacgctg
     4861 gttgttggcc ggcgggtggc gaaccatcag ccggcgcagc gcgcgcgctt cggcgatcag
     4921 cggctcgggg ccgaccatgt agccgagccg cagaccgggc gccagcgtct tcgacaggct
     4981 gccgacatag atcacgcggc cggtggcgtc gaggctcttc agcgccggcg tcggctcgcc
     5041 gacgaaattg atttcgctct cgtaatcgtc ttcgacgatc aggaaatcgt gtttctcggc
     5101 ctgcttcagc aggcgttcgc gacgctccag cggcatggtt accgtggtcg gcgactggtg
     5161 gctcggcgtg gtatagacga attcgcaggc attcatcgac tggccgggaa ccacgccctg
     5221 ctcgtcgacc gccagcggca ccatgcgcgg tgccttgagc tcgaagatgt tgcgcgcatc
     5281 cgggtacccc gggttttcca tgccgacgcg cgtgccgcgc tcgatcagca ggtcggcgag
     5341 aatatacaac gcctgctgcg cgcccatcgt aatcaggatt tcgtcgcgcg ccgcccacac
     5401 cccgcggcgc ggtagaatgc gcgcctggat ctgctcgatc agcagtggat cgtcggcatc
     5461 gatgcggtcc ggcgtgattt cctgaatatc gctgacgcta agcgcctggc ggcagcattc
     5521 gcgccagtcg gcaaccggga acaggcccgg gtcgagctgc ccgaacagga acgggtaggg
     5581 atacttcttc cagtccggcg gtttgacgat attgcgctgt cgcgacggat ggaacttgat
     5641 atgccgctgc cagtccggcg ccggatgttc gccgtcgggc tgcagcggtt cgacccgcac
     5701 gcggccctcg agcatggtct tgtcgatgta gtaaccgctg cgttcgcgcg cgaccaggta
     5761 gccctcgtca atcaattgct ggtaggccag caccacggta ttgcgggcaa cgccgagatg
     5821 gcgcgcgagt tctctcgacg acggcagtgg caggtcgagc ggcagtttgt cgtcaagaat
     5881 cgcgctgacg atcatctccc gcagctgccg ctgatagctg accttgcggc cacgcgacag
     5941 ctgaaacagt cgctcccaca taatctgctc gccacggttc gcctgtggca ttaattggtc
     6001 tcatagatag gcataatctg gctctattgt aactgcttcg attgcattta gcctcttatt
     6061 cccttcaatc cttgtgcaga cgcccgtggg cgcggagcag gctatgaaac ccgaaaacct
     6121 caacgatatc gtcggcagcg atcgtaacca tctctggcat cacctgatgc agcacaaacc
     6181 ctggcagtcg agcgatccga tggtcatcgt cgagggcaag ggcttgcgcg tcaaggatgc
     6241 caacgggcgt gagtacctgg atgcggtatc gggcggcgtg tggaccgtga acgtcggtta
     6301 cggccgcgaa agtatcgccg acgcggtgcg cgagcaaatc gccaagatgt gctatttcgc
     6361 caattctgcc ggtaatattc cgggcgcgca gttcgccgag aagctggtcg acaagatgcc
     6421 cggcatgagc cgcgtctatt acgccaactc gggctcggaa gccaacgaaa agggtttcaa
     6481 gctggtgcgc cagctcgcgg cgctgaaaaa cgatggcaag aagcacaaga tcatctaccg
     6541 cgatcgcgat taccacggca ccacgatcac cacgctgtcg gccggcggcc aggcgcagcg
     6601 caaggaccag tacggcccgt tcacgccggg cttcgtcgag atgccgcact gcctcgctta
     6661 ccgcgatccg cgcggcgagg atcccgatta cggcgtcaag gcggcgaagg acctggaaaa
     6721 agtcattctc gccgaggatc cggataccgt cggctcggta tgcctggagc cgatcaccgc
     6781 tggcggcggt gtcatcgtgc cgcccgaggg ctactgggaa accgtgcagg aaatctgcag
     6841 caagtatggc gtgctgatcc atatcgacga ggtggtctgt ggtatgggcc gtaccggcaa
     6901 gtggttcggc taccagcact tcggcgtcaa gccggacatc gtcaccatgg ccaagggcgt
     6961 cgccagcgga tacgcggcga tctcggtaat ggtcaccacc gaagacctgt tcaacgaatt
     7021 cctcgccgaa cccagcgatc agctgcacta cttccgcgac atcagcacct tcggcggctg
     7081 cgccggcggc ccggctgcgg cgctcgaaaa catgcgcatc atcgagcagg aaaacctgct
     7141 cgacaatgtc aaccgcatgg gcgattacct gatggatcgg ctgtacgaac tgcaggacaa
     7201 gcatgccgtg atcggcgacg tgcgcggcaa gggcctgttc tgcggcgccg agctggtcag
     7261 tgaccgtgaa acccgccagc ccctcgacga gagcctggcc gccgcggtgg tcgccgactg
     7321 cctgaagcag ggggtgatga tcgggcgcac caaccgcagc ctgccgcagt tcaacaacac
     7381 gctgtgcctg agcccggcgc tgatctgcac gcgggacgac atcgacgaaa tcgtcgcggc
     7441 gatcgatgtg gcgctgggga atcttgcaaa ctagcagccg gcggggataa tcccctgatc
     7501 atgattcgcc tattgctgct gatcgccgcg ctgagcgcgc tagtcccggg caccgttgtc
     7561 tccgccgcgt tggataagcc ggagaaaatc tcggtcggct atctgccgca ttggccgacg
     7621 ccggtgcagt tcgcgcgcat caagaatacc ttcgatgtcg cgctcggcat cgaggtcgac
     7681 tgggtggcct ttgaagacgg caaccagatg agcgacgcgc tggcccgtgg cgatattcag
     7741 atcgctcatt cccagggaca catccccttc ctggccgctg tcagccgcgg actcgacctg
     7801 accatggtcg gggtcgcggt ggactacgcg gaggacgata actgcatcgt gcgcagcgat
     7861 acgggtatcg gccgcggcac ggcggcgctg ctggccggca accgggttgc ggtgcgcacc
     7921 ggcagtatca gccattttcg aatgctcggc gtattgcgcc acctcggcgt cgatccggca
     7981 caggtcgaga tcgtcgcggt ggcccgcgac ggcgacgccc gtgcggccct cgaaagcggc
     8041 gacgtggtca tggcctgtgc gtccggcgcg gtgctgcgcg agttgcgctc gctgggagaa
     8101 ccgttgatga atggcgtcgg gcagtcggct atcggactgc gcgtgttcga ctcgatcacc
     8161 gtcgccagcg atttcgtgca ccagcatgcc gatatcgtgc aagccttcat ggatatcgtc
     8221 gaggcgtcca acaagcaatg gcgacagaat ccgcagtcga tgctgaaagc gatagcgcgc
     8281 gccaccgacc tggatctggc cagtacccgg caggcgctcg cgggcttcaa ctttccgacc
     8341 gcgatggaac agaaatcgga cgcctggctg ggtggcgacg tggtcgacta cagcaaggag
     8401 ctggccgatt tcttcgtcgc cgagggtgtg ctgccgcggg cgctggaaag ctacgagcgt
     8461 ttcatcacca cgcgctttct gcgctgaatg cccggccacg gcggcggcca gcgcttgcag
     8521 gcggaatcga cagcgcggga agcggcggca ctgcaagtct tcgacattac cgagcggctt
     8581 gccgcggagg atttcctgtt catcgaggcc gcgcgcacgg tcgagttgct ggcggccatc
     8641 tcggccaccg atatcgtcaa tgacgaggtc tttctggaca gctggaatcg cctcgaggaa
     8701 gaccgctaca tggccgatgg cggcaagtac cgcaagcgtc ggcacgcggt ctacgcaatc
     8761 cacaacccgg gtgagacggc acggcaaatg ccctaccagc cgcactacca gaccctcgac
     8821 tacaacccgc tgaacggtgg ggtggcgcgc tatttcgcgc cgattctgga tgacttgcat
     8881 cacagcccga cgcttgcggc gctgctcgaa ttcggtaacc gtgtgtttag ccggatcagc
     8941 ggcaatcagc agtggcacat cgaattgcac cagtttcgca tcgaggcgcg cgacggcagc
     9001 ctcggcaagc cgacgcccga gggcgcgcat cgcgacggcg tcgatttcgt tatcgtggtg
     9061 atgatcaagc gcgtcaatat cgacagtggt gcgaccacga ttttcaattc cgagcatcaa
     9121 ctggtcggcg aattcatgct gcgcgagact ttcgatatgg tgctggtcaa cgatcgccgg
     9181 gtctaccacg gcgtaacccc gatcacccag ctcgacgccg gcctcgaagc ttatcgcgac
     9241 gtgctcgtcg tcaccttcaa gaaaccggac aacagccagg caggggcgta gcatcggcgg
     9301 tgggaaccgg cgataggcgg tggaatcgct tgccgctgcg gatacgcttt catgaatgtc
     9361 gtatctcggc aagtaccccg tctggccagg agtatattgt tcgggaccgc gtgtgaattt
     9421 agctcgcggt tactttagaa ttcgccacta caggcacaat cgactcggga ctaatcgtgg
     9481 aaagagaagc aatgaatttt gacgtggtca tcgtcggcgc cgggccggcg gggctgacca
     9541 tgggtattcg cctcgcgcaa ctggcgcgcg aacaggaacg cgagctgtcg atctgtattc
     9601 tcgaaaaagg cgccgaggtc ggcgcgcaca tactttccgg cgcggtaatc gagccgcgcg
     9661 cgctcgacga actgctgccg gactggaagg aacgcggcgc gccgctgaac gtgccggtaa
     9721 aagccgacca gttcaagtac ctgaccgcga agggtgcgat ctcgatgccg accccgccgc
     9781 agatggacaa cgaaggcaat tacatcatca gcctcggcaa cttctgccgc tggctcgccg
     9841 agcaggccga ggaactcggc gtggagattt ttcccggttt tgccgccgcc gaggtgctct
     9901 acgacgaggc cggcgcggtg cgcggtgtgg ccaccggcga catgggcatc ggcaaggacg
     9961 gcgagccgac cgacggttac gagccgggcg tcgagctgat tggccggcaa accgtgttcg
    10021 ccgagggttg ccgcggttcg ctaagcggcg agttgatgca gaaattcggc ctgcgcgacg
    10081 gtgtcgagcc gcagacctac ggcatcggca tcaaggaact ctgggaagtc aggcccgagg
    10141 tgcatgccga gggaaccgcg ctgcacacca tcggctggcc gctgcagtcc gacacctacg
    10201 gcggctcgtt cctgtaccac aacgaaaaca accaggtcgc ggtcggtttc gtcatcggcc
    10261 tcgattaccg taatccgcac ctgagcccgt tcgaggaatt ccagcgcttc aagacgcacc
    10321 cggcgattcg cccgctgttc gagggcggca agcggatcgc ctacggtgcg cgcgcgatct
    10381 ccgagggcgg cctgcagtcg ctgccgaagc tgacctttcc gggcggcctg ctggtcggcg
    10441 ataccgcggg tttcctgaac gtgccgaaga tcaagggtac gcatacggcg atgaagtcgg
    10501 cgatgctcgc cgccgaggcc gtattcgaat cgctgccggc cgactacgac gaggaaaacc
    10561 ccgggccggt actcgaggcg accgcctacg cggacaagtt tcgccagtcc tggctttacc
    10621 gggaattgaa ccaggtgcgc aacattcgcc ccggtttcca tcgcggcctg tggtggggtc
    10681 tggttctcgc ggcgctcgaa tcctacctgt ttcgcggcaa ggcgccgtgg accttcgggc
    10741 atcatgccga tcacgagcaa ctcggccaca agaatgatta cccgaagatc gattacccga
    10801 aaccggacgg cgaagtcagc ttcgaccgca attcgtcggt ctacctgtcg ggcacttacc
    10861 acgaggaaaa ccagcccgcc cacctgcggc tgcgcgatga cagcgtgccg ataacgctga
    10921 acctggaaaa atacgatgcg cccgagcagc gctactgccc tgccggggtc tatgagatcg
    10981 tgcagcgcga cggccagccg gcgttacaga tcaacgcgca gaactgcgtg cactgcaaga
    11041 cctgcgatat caaggatccg agccagaaca ttcactgggt ggtcccagag ggcggcggcg
    11101 gcccggctta tccgaatagg tgacgactgc gcattacgag atacccgctt gcgcgggcat
    11161 gactccaggc gggtcaatca cggcgcaagc cgcacccggc ccggcgggga tcttgcaacg
    11221 actgcgcatt acgagatgcc cgcttgcgcg ggcatgactc caggcgggtc attcaccgcg
    11281 ccagccgccc ctgttaaact gggtcgaacc caccagccgc gagattccgc taatgccgca
    11341 cctgattatc gagtactccg agggcgtcga cggcactgtc gcgattcccg acatcgtcga
    11401 tgccgcccac gccggcgcga tgagttcgga gctgtttccg gaatacgaca tcaagacccg
    11461 cgcgatcggc tatcgcgatc accgcaccgg cgcgacgcgc gattccttcg tgcacgtgac
    11521 ggtgagactg ctcgacggcc gcagcgacgc gcaaaaagcg atgctcagtg aggccgtgct
    11581 cgcgcatatc gaaccgctgt tacccggggt gaacagtgtc ggtgtcgaga tcgtcgatat
    11641 gcaccgcgcc agttatcgca agcgggtgct cgacggttga gcggaattta atcgcgctgc
    11701 cggccggcga acagcgccgc ggtcgcggct gcaggttcga ggtgccgcaa ttcggcgagc
    11761 agctgccgga ccgcgtccag atcggtaccg aggtacgggt tgtccggctg ttgcgcgcac
    11821 tgatgagcgc cgatgcacag gctcatgcac aggcgcatgc agatgaaatc gaagaccgtt
    11881 tcggcctccg ccggctgcaa tggcatgcgc tcgcaatagc ccgcgagcag tgcggcggcc
    11941 gcttccagcg gctgcgcgcg gtcgagcatc acgtaagtcg ccgcgatcga cgggtcgacc
    12001 gcgagccaac cttccagcat gtcgccgaag tcgatgatgc tgagcaggtg cgccgcggat
    12061 tcgtcgacca ccaggttgtt acgattggcg tcgttgtgga tcaccgtgcg gcgcaattcc
    12121 gcgatgcgcg gtacgacccg ccgccgataa ccgtcggcga agtgatccgc cagcgcgcgc
    12181 gactcagggt cgagcaacgg gctgtagccg tcgacgatct cgagcgccgt gtccatcttc
    12241 cacaacaagg ggcgttccag cgccgcgtgt gaaaagccgt ccagcgcccg atccagcaat
    12301 gccagccgac atccgatatc ggcgagcagt tgcgctgacg gattttctat ctcgcgaaac
    12361 aatcttccct cgacgtaggg cagcacgcgg cagacatgcg catggccgtt ggcgtcacgc
    12421 acgctctcga tcgttttgcc gttgaccgat tgcctggccg tcaccgttgc cgggaatacg
    12481 ccagccgcgg cgagccgttc gaaaacctgg tgctggcatt cgagcatcgc cggcgactcc
    12541 tcggcattgg cgatcttgaa gacgaagcga tcgccgggcg caccgatcag gaaattcagg
    12601 tcgcgctcgc cgtcgagtcg tcgaatcggg ccgtcgattg caaacagccg gcgagcgata
    12661 tccgcggcct cgtcgagctc gaattcgggc agcaaacaat tgtcggtcat cgcgatctgg
    12721 attcgggtac gaatttcgtc cctagcagca tcgcccgcaa atgaaaataa attttgcttc
    12781 gagcggcatt gtgattgctg ttttaatttg gatctgaccc aaattatgat cgctgtcccg
    12841 ggttatcttc ggctacaaga ggccgttgag caatgtcgca attggttaaa acgaggccgt
    12901 agacggattt cggggctgca tatatagtcg ctgaaaacac gggagtattc atggcattac
    12961 ggctcggtat cgataccggc ggaaccttta ccgacgcggt gctggtggat gacgacaagc
    13021 gcatcgtcaa cgccgagaaa tgcctgacca cgcgcttcga cctgagtatc ggcatcggcg
    13081 acgtggtcgc gcggctgccg gccgcgtcgc tcgccgaggt gatcatggtt tcgctgtcga
    13141 ccacgctgac taccaactcg gtggtcgagg atctcggcgc gccggtctgc gtgctgctgc
    13201 cgggctacaa tcccgagcaa atcaagcaga gcgggctgct caatattctg ccggcgcagt
    13261 tcgtggtgcg cctgcagggc ggtttcgatg cggtcggccg cgagcatgcg ccgctcgacg
    13321 aggccggcgc gcgcgaggcg atcgaacggc ttcaggacga ggtctcggcg tttgcgatct
    13381 cgagtatgtt cggcacgcgc aattccagcc acgaaaaccg cctcaagcag atggtcgccg
    13441 agattaccgg tatgccggtg atctgcggcc acgaactggc ctccagtctc ggtgcgccgc
    13501 gacgtgcgct gaccgcggcg ctgaacgcgc gcatggtgcc gccgatcaag gagctgatcg
    13561 gctcggtaca gaaaatcctc gatcgtcacc gcatcgacgc accgctgatg ctggtcaagg
    13621 gcgacggttc gctggccagc cttgaaaacg cgatcgaaaa accgatcggc acggtactct
    13681 ccggcccggc ggccagcgtt atcggcgcct gccagctgtc gggcctggaa aacgcgatcg
    13741 tcgccgacat gggcggtacc actaccgata tcgcgatcgt cagcaacggc cagccacagc
    13801 tcagcgaaga gggtgccaat atcggcgact ggcaaccgat ggtagaagcg gtgcgggtca
    13861 attccatcgg tctcggcggc gatagtgaaa ttcgtttcgg cggccacacc ggcctctcgg
    13921 tcgggccgcg gcgggtggtg ccgaccagcc tgctcggtca tctctacccg gaaatcattc
    13981 cgcggctcga ggcccagctg gccggcatga ccagtgcccg cagcaaccgc ttcgtgatgc
    14041 gcctcgaaag caatccggcg ctgctcaggc agctcggcaa gatcgagcgg gaagcctggg
    14101 aactgctgtc cgacagaccc gtcgaactcg atgccatcgt cgccgagaac cgcgcttacg
    14161 cgcgcgcgct ggccaggctc aagcgcatgg gattggcgat ctacagcggc ttcaccccca
    14221 ccgacgcggt gcacgtgctg ggcatcaact cgcactggag caagcccgcg gcggaactgt
    14281 cggcgcgcct gtggatgcgg cagatgcgct ttctctacgg ctacggcaaa tgggacagcg
    14341 acgatatcga aaccgcctgc cgcgaagtct acgaactcgt caacgccagc attcggcgcc
    14401 tgctgatcga ggtcgggttg aacgaattca gcctcgtcgg caagactggc aagagcggcg
    14461 cggtcgtcga taccatcacc cgtatggtgc tcggcagcca tcagcaccag ggccgcaagc
    14521 cattgttcaa tctcgatttc gccagcgatt acccgatcgt cgcggtcggc gcaccggcgg
    14581 cgagctatta tcgcgacgtg gccgacagca tgggtatcga gctgtatctg ccggagtatg
    14641 ccgaggtggc caatgcgttt ggcgcggtga tgggcagcgt gatccagcgc gcccaggtta
    14701 ccgtcaccca gccccagcac ggtatctttt gcctgtttca cgaagaccag ccgaaaaatt
    14761 tcgatactct cgaggccgcc aagcaggccg cgcaggagat cgtttcgcgg ctggcgcagc
    14821 aacgggcgct cgccgccggc gcgatcgagc cgaccgtgac cctggctttc gaggagctgc
    14881 acgtcaagga caaggtcgac ggcgaactgt ttctcgaatc caccgtgatc gcgaccgcca
    14941 tcggccgcgc ctgcgactgg cgcaccgagc gctcggcatg aacctcgacc ggcgcctgcg
    15001 tgatcacggg ctgttcgtgc gcggcgtgac ccggctagcc gacgacgaga tcgatgccta
    15061 cgggttggcg gccgaccggc cctgggtggc gctggtcggc aatatcggtt ccagttactg
    15121 gagtgcgttc agcgctgccg acgagtaccg cgacggcgcc gccgacccgc tggatcgctg
    15181 gagccggcgc atcgccgaaa cgcttgcctc cgagttcgcg ctgcaggcga tttttccttt
    15241 cgaagggccg ccctactatc cttttcagcg ctgggcgcgg cgcgcggagg ggctcgcgca
    15301 atcgccgctc ggcatcatga tccaccccga gcacggcc
//
LOCUS       DATCZV010000303         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_394658, whole genome shotgun sequence.
ACCESSION   DATCZV010000303 DATCZV010000000
VERSION     DATCZV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394658"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..950
                     /locus_tag="VIW27_06765"
     CDS             126..950
                     /locus_tag="VIW27_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5739411.1"
                     /translation="MSTIWKFLVLAACVYAGITALIYFRQSSLIYYPNVSGRELAASP
                     QYIGLAYEDVELLTEDGVRLHGWFVPGENARGTLLFFHGNAGNISHRLDSIAIFNRMN
                     LDVFIIDYRGYGQSEGRSSETGTYRDAEAAWLYLRETRGIGADRIIVFGRSLGASIAA
                     WLARRHQPAALIVESGFSSVPSMAQRLYPFLPVKWLSRFSYDTRQYVSAIACPLLVAH
                     SRDDEIIPYAEGRLVFDAAPANKRFLEMRVGHNDGFIVSGSTYSDGLARFIAGALN"
     gene            1274..1780
                     /locus_tag="VIW27_06770"
     CDS             1274..1780
                     /locus_tag="VIW27_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5739412.1"
                     /translation="MIHFSRLSAVVLLLPLLVSCMRPEDHFDSEYRSAPYVANGERIY
                     FTGTSSSGKAILATGGSGMMGMHRQMHGGGCAICHGGNREGKRMWPRFWLKAPALTPA
                     ALFEDSHADHGHQDHAGYDADSLRLAIIAGRSASGEALDIAMPRWTMSAADLDDLVAY
                     LQQDHRHD"
     gene            complement(1845..>2195)
                     /locus_tag="VIW27_06775"
     CDS             complement(1845..>2195)
                     /locus_tag="VIW27_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739413.1"
                     /translation="AFSRVSCDDFNNSDASMWVRYIDSGERRTLDVVLKMPATDQNQA
                     KSNRSVLLGTQNIGQITLTPNNEGMLSGSLSYDSYASSGGSNPGVSAFPGSWTGAVSG
                     TLVRTSGLACELRD"
BASE COUNT          379 a          724 c          679 g          413 t
ORIGIN      
        1 gatgacttcg ggagcgggga tgacttcggg agcggggatg acttcgggag cggggatgac
       61 ttcgggagcg gggatggcgg caccggagta cactggcggc acattcgaag cgcggtgaac
      121 aacgcatgag tacaatctgg aagttcctgg ttctcgcggc ctgcgtctac gcaggtatta
      181 ccgcgctgat atattttcgc cagtcgagcc tgatctacta cccgaatgtc tccggcaggg
      241 agctggccgc ctcgccgcaa tacatcggcc tcgcctacga ggacgtcgaa ctgctgaccg
      301 aggacggggt tcgcctgcat ggctggttcg tgccgggcga aaacgctcgc ggcacgttgc
      361 tgttctttca cggcaacgcc ggcaacattt cgcaccggct ggattccatc gcgatcttta
      421 atcgcatgaa tctcgacgta ttcattatcg actatcgcgg ctacggccag agcgagggtc
      481 ggagcagcga aaccggcacc taccgcgacg ccgaggcggc ctggctttac ctgcgcgaga
      541 cccgcggcat cggcgcggac cggatcatcg tcttcggccg ctcgctgggc gcgtcgatcg
      601 ccgcgtggct cgcgcgccgc catcaaccgg ccgcgctgat cgtcgagtcg ggtttcagct
      661 cggtgccgtc gatggcacag cggctatacc cgttcctgcc ggtcaaatgg ctgtcgcgat
      721 tcagctacga cactcggcaa tacgtcagcg ctatcgcttg cccgctgctg gtggcgcaca
      781 gcagggacga cgagatcatt ccctatgccg aggggcgcct ggttttcgat gcggcaccgg
      841 caaataagcg atttctggag atgcgcgtcg gccataacga cggtttcatc gtctccggca
      901 gcacttacag cgacgggctt gcccgtttca tcgccggcgc gctgaattga taaccgggcc
      961 atttcggcgc cgccgaacgc cgcgcggtgg atatcccgca gccacccggc attaccagct
     1021 acccgcttgc gcggcgctgt cttcggcttg gtgcggagat gactgcagcg agagagtcca
     1081 ctaccgggtc ggccgctttc gcggcgttcg accgtggcat gctccgagct ccgcgactcc
     1141 cgcatgcgca accgtgcgca ccggcaatcc cctgcccggc tacagccagc ctggccatgc
     1201 agcgcgcggg tcaaaccccg cgcaacccct tctggcaaaa tagcaactgg ttccgatcgg
     1261 gagatctctc gccatgatcc atttttcgag actcagcgcg gtcgtgctgt tgctgccgct
     1321 cctcgtttcc tgcatgcgcc cggaagatca cttcgactcc gaataccgct cggcgccata
     1381 cgttgccaac ggcgagcgca tttatttcac cggcaccagt agctccggaa aagcgatcct
     1441 ggcaaccggc ggcagcggca tgatgggcat gcaccggcaa atgcacggcg gtggctgcgc
     1501 catctgccac ggtggcaacc gcgagggcaa gcgcatgtgg ccgcgcttct ggctaaaggc
     1561 gccggcgctg acgcccgcgg cattgttcga ggacagccac gccgatcatg gccatcagga
     1621 tcacgctggc tacgatgccg attccctgcg cctggcgatc atcgccggac gctcggcctc
     1681 cggcgaggcg ctcgacatcg caatgccccg ctggacgatg agcgctgccg acctggatga
     1741 cctggtcgct tacctgcagc aagaccaccg ccacgattag cctatcgcag gccctccggg
     1801 ccgactcgtc gaccggtgac gactagccac cggcttcgct gcgcctagtc gcgcaactcg
     1861 caggcgagac ccgacgtcct gaccagggtt ccgcttaccg cgccggtcca gctgccggga
     1921 aaggccgaca cgccgggatt agacccgccg ctggacgcat acgagtcgta cgacaggctt
     1981 ccgctcaaca tgccttcgtt gttcggcgtg agggtgatct gcccgatatt ttgggtcccg
     2041 agcagcacgc tgcgattgga ttttgcctgg ttctggtcgg tagccggcat tttcagcacc
     2101 acatcgagcg tgcggcgctc gccgctgtcg atgtagcgca cccacatcga cgcatcgctg
     2161 ttgttgaaat cgtcgcaact caccctcgaa aaggc
//
LOCUS       DATCZV010000304         5980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_342096, whole genome shotgun sequence.
ACCESSION   DATCZV010000304 DATCZV010000000
VERSION     DATCZV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342096"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VIW27_06780"
     CDS             complement(<1..699)
                     /locus_tag="VIW27_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5739414.1"
                     /translation="MPRFNRLLITGAAGNLGRELRAGLAPLAATLRLTDRGEMAPAGD
                     NEEVMQAELGDFDAIMKVVEGCDAIVHFGAAPVERPWEEILESSIKGGYNVYEAARRH
                     GVKRIVYASSIHAVGYERREVGADTDTPHNPDTLYGVSKCFVEDLAKLYFNKWGIESA
                     CLRINSCFPTPADRRHLATWLSFRDLVQLVERCLVSERIGHTVVYGISNNREAFFSNH
                     KVAHLGYRPEDSAED"
     gene            1177..2856
                     /locus_tag="VIW27_06785"
     CDS             1177..2856
                     /locus_tag="VIW27_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY5739415.1"
                     /translation="MNKPSAESSGGSNFIRDIIDADLASGKLSRVVTRFPPEPNGYLH
                     IGHAKSICLNFGIARDYGGDCNLRFDDTNPAKEEQEYIDSIRRDVEWLGFEWHSNPFA
                     SDYFDQLYAWALDLIRQGKAYVDSQSAEQIRANRGTLTEPGKNSPYRERSVEENLQLF
                     EDMKNDRYADGEHVLRAKIDMGSPNINMRDPVLYRILHATHHNTGDDWCIYPLYDFTH
                     GLSDAVEGVTHSLCTLEFEDHRPLYDWILDNLETPSRPVQIEFARLQLEYSLTSKRKL
                     NQLVREGIVDGWDDPRMLTISGMRRRGFTPQSIVKFCDMIGITKKDSTIEMGVLETAV
                     RDDLNARAVRRMAVIDPLKVIIENYPEDQVEYFEGANHPQNPQYGTREVPFAREIYIE
                     RDDFMEQAPAKFFRLSPGREVRLRFAYYITCKEILRGDNGEIEALICSYDPDSRGGNS
                     PDGRKVKGTIHWVSAQHAVPATVNLYDRLFNSPNPGAAADFHQALNPESRMVMANARL
                     EPGIELAGNTLPYQFERLGYFISDYDSTAEQPVFNRTMTLRDSWAKIEKSG"
     gene            2858..3724
                     /locus_tag="VIW27_06790"
     CDS             2858..3724
                     /locus_tag="VIW27_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739416.1"
                     /translation="MGKPLVQRWLELLSHLSLRGLYRLGDMLALAAVSIPNDVTRTAR
                     RNIDLCFPELDAAERRRLYRAAIRHTCYSAVEMAAVWCWPVDKIRAQITEAEICDSFE
                     RSTRGRIVLLPHLGSWETLAAWLGQRMSVMYLYKKNPNPALDEFIMQARARTGGEPVP
                     TEKHGLRQLLVGLRRGNSAVILPDQRPRRHRVQIESTFFGLSAPTTTLVQNLCTKLDC
                     DVFIAVVYRSSPAGHFGVCIEALDRARLAAEPEASAAYMNEQIEMRVRRFPEQYNWGY
                     RRFDNQLYESTD"
     gene            3728..>5980
                     /locus_tag="VIW27_06795"
     CDS             3728..>5980
                     /locus_tag="VIW27_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5739417.1"
                     /translation="MRKLFSRITSGYDLLVLRQPVATLIALCALIAYFLVYIPDFELD
                     ASADSLVLENDSSLKYYRSIRERYGSDDFLIVTYTPDGELFSQPVLDDIASLRDALAA
                     LEQVDSVVSLLDVPLVDSPPMTLAEISRGVRTLQSDDADVQLAEREMRSSALYENLLV
                     NSSGDTTAMQVNLVVNRKLLDLIKSRDLLYEEAGHHPQAQQRIEELSAQIQIENQLQK
                     ERLERTIAEVRGIMDGYRDRATLFLGGVPMIASDSIAFIRSDLRVFGVGVVLFIIVIL
                     AFSFGHSRWVALPLAVCLASAMTMMGYLGWAQWPVTVVSSNFISLLLIITLSLIIHLV
                     VRYRELAGLDAAASQYELVRATVHSKFAPSFYTAITTMVAFGSLLVSGIRPVIDFGWM
                     MVIGITVSFVLAFTLFPAALTLLPRLETRIKKDYTGKVTGAIADFIQHRTRATLLIFV
                     ALVAIALGGIPRLSVENRFIDYYKEDTEIYQGMTLIDQRLGGTTPMDIIIDAPPGALP
                     DEDDLFGGNDITANSYWFNRDGLEQAALIHDYLDKLPETGKVLSVHTGMQLLESLNDD
                     QPFTDFKLAVVYKRLPDSVKQALIEPYLSDDGNQLRFSLRLYESDRSLRRQALIDSIY
                     RDLINDFELDPEQVTISGMAVLYNNLLQSLFRSQILTLGAVFGAILLMFIALFRAFRL
                     SLAAIVPNIIAAGLILGLMGWIGIPLDIMTITIASVSLGIAVDSAIHYVTRFRREVRA
                     TGDFEAATRAA"
BASE COUNT         1150 a         1960 c         1775 g         1095 t
ORIGIN      
        1 atcctcggcg ctgtcctccg gccggtaacc gaggtgcgcg accttgtggt tggagaaaaa
       61 ggcttcgcgg ttattcgaaa taccgtagac cacggtatgg ccgatgcgct cggacaccag
      121 gcagcgctcg accagctgca ccaggtcgcg aaacgacagc caggtcgcga gatggcggcg
      181 gtcggccggc gtcgggaagc aggaattgat acgcaggcag gccgattcga tgccccactt
      241 gttgaaatac aacttcgcca gatcctcgac gaagcacttg gagacgccat acagcgtgtc
      301 cggattatgc ggcgtgtcgg tgtcggcgcc gacctctcgg cgctcgtagc cgaccgcgtg
      361 aatcgaactt gcgtagacga tgcgcttgac gccgtgccgg cgggccgcct cgtagacgtt
      421 gtagccgccc ttgatcgaac tctcgagaat ctcttcccag ggccgctcga ccggggccgc
      481 gccgaaatgg acgatcgcgt cgcagccttc gaccactttc atgatcgcgt cgaaatcgcc
      541 gagttcggcc tgcatgactt cttcgttgtc gccggccggc gccatttcgc cacgatcggt
      601 gagtcgcagc gtcgccgcca gcggcgccag cccagcgcgc agctcgcgac cgagatttcc
      661 ggcggccccg gtgatcaaga gtcgattaaa acgtggcatg tcactctcct gtttttaagt
      721 ttgcactatt gtatctcaat cactgcgcgc ggcgtcagtt cggccgccag ccattgccga
      781 gcgcacacgc cctgttggcg gcagtacgcc atatcgagtc gagcccgcaa tgacttccaa
      841 tattcgtcgc cccggcgccg cccgcggccc gcccgagccg gggcccatcg caaagccggg
      901 caggcagtgt tgcggagcac tgctcgctcc cggcgctcgc catcgactgc cgtttagaga
      961 tagattgcgg ctctacgaag tatcgggcaa gaccgcaatg acaggtgtaa gccgttgccc
     1021 cggccaatga cccggggttc attcgacgtt ttttgcagct gcccgccagg agacgccggc
     1081 cgatacacgg caaacggcgt caggccggta aatacattca gctttgctgt agtatcgggt
     1141 aaaatcctgc gattcttttc gacgtcgatc tctcacatga acaaacccag cgccgaatcc
     1201 agcggcggca gcaactttat tcgcgatatc atcgatgccg acctcgcctc gggcaagctg
     1261 tcgagagtgg tcacgcgctt tccgcccgag cccaacggct acctgcatat cggccacgcc
     1321 aagtcgatct gcctgaactt cggcatcgcc cgcgattacg gcggcgactg caacctgcgc
     1381 ttcgacgata ccaacccggc caaagaggag caggagtaca tcgactcgat tcgccgcgac
     1441 gtcgaatggc tcggattcga gtggcacagc aacccgttcg cttcggatta cttcgaccag
     1501 ctctacgcct gggcgctgga cctgatcagg cagggcaagg cctatgtcga cagccagagc
     1561 gccgagcaga tccgcgccaa tcgtggcacc ctgaccgagc ccggcaagaa cagcccttac
     1621 cgcgagcgca gcgtcgagga aaacctgcaa ctgttcgagg acatgaaaaa cgaccgttac
     1681 gccgatggcg aacacgtgct gcgcgcgaaa atcgacatgg gctcgccgaa tatcaacatg
     1741 cgcgatccgg tactctaccg catcctgcac gcgacccatc acaataccgg cgacgactgg
     1801 tgcatctacc cgctgtacga ctttacccac ggcttgtcgg acgccgtcga gggcgtcacg
     1861 cactcgctgt gcacgctcga gttcgaagac caccgaccgc tctacgactg gattctcgac
     1921 aatctcgaaa cgccgtcgcg cccggtgcag atcgagttcg cgcgcctgca gctcgagtat
     1981 tcgctaacca gcaagcgtaa gctgaaccag ttggtccgcg agggcatcgt cgacggctgg
     2041 gacgatccgc gcatgctgac gatctccggt atgcgccggc gcggtttcac gccgcaatcg
     2101 atcgtcaaat tctgcgacat gatcggcatc accaagaaag acagcaccat cgagatgggc
     2161 gtgctggaaa ccgcggtgcg cgacgacctg aacgcgcgcg cggtgcggcg catggcggtt
     2221 atcgacccgc tcaaggtgat catcgagaat taccccgagg accaggtcga gtacttcgaa
     2281 ggcgccaacc acccgcaaaa tccccaatac ggcacgcgcg aagttccgtt cgcccgcgag
     2341 atctacatcg agcgcgacga tttcatggaa caggcgccag cgaaattttt ccgcctgagc
     2401 ccggggcgcg aggtgcgcct gcgctttgcc tactacatca cctgcaagga aatcctgcgc
     2461 ggcgacaacg gcgaaatcga ggcgctgatc tgcagctacg atcccgacag ccgcggcggc
     2521 aacagccccg acgggcgcaa ggtcaagggc accattcact gggtcagcgc tcagcatgcc
     2581 gtaccggcga ccgtgaatct gtacgatcgc ctgttcaatt cccccaatcc cggcgcggcg
     2641 gcggattttc accaggcatt gaatcccgag tcccgcatgg tgatggcgaa tgcccggcta
     2701 gaacccggta tcgagctcgc cggcaatacc ctgccctacc agttcgagcg ccttggatac
     2761 ttcatcagcg attacgattc gaccgccgag caaccggtct tcaaccgcac catgaccctg
     2821 cgcgacagct gggcaaaaat cgaaaagtcg ggctgagttg ggcaaaccgc tggtccagcg
     2881 ctggctcgag ctgttgtcgc atctgtcgct gcgcgggctc taccgccttg gggatatgct
     2941 cgcgctggcc gcggtcagca tacccaacga cgtgacgcgc acggcgcggc gcaatatcga
     3001 tctgtgtttt cccgagctcg acgcggccga gcgcaggcgg ctgtatcgcg cggcgattcg
     3061 gcacacctgc tacagcgccg tcgagatggc ggcggtatgg tgctggccgg tggacaaaat
     3121 tcgagcacag atcaccgagg ccgagatctg cgacagtttc gagcgctcca cgcgcggccg
     3181 catcgttttg ctgccccacc tcggcagctg ggaaaccctg gcggcgtggc tgggtcagcg
     3241 catgagcgtc atgtacctgt acaaaaagaa tccgaacccg gcgctggacg aattcatcat
     3301 gcaggcgcgc gcgcgcaccg gcggcgagcc cgtgccgacc gaaaagcacg gcctgcgcca
     3361 gctgctggta ggactcaggc gcggcaacag cgcggtcata ctgcccgacc agagaccgcg
     3421 gcgtcatcga gtgcagatcg agtcgacgtt cttcggcctg agcgccccta ccacgaccct
     3481 ggtgcaaaat ctgtgcacca agctagactg tgacgtattc atcgcggtgg tgtatcgcag
     3541 ttctcccgcg ggtcacttcg gcgtctgcat cgaagcgctc gaccgcgccc gcctggccgc
     3601 cgagcccgag gcgagcgcgg cctacatgaa cgagcagatc gagatgcgcg tgcgacgctt
     3661 cccggagcag tacaactggg gatatcgccg cttcgacaac cagctatacg agtcaaccga
     3721 ctagtccatg cgcaagctgt tttcgagaat cacgtcggga tacgacctgc tggtgctgcg
     3781 ccagccggtc gctacgctga tcgcgctgtg cgcgctcatc gcctacttcc tggtctatat
     3841 tcccgatttc gagctggacg cctcggccga ctcgctggtg ctggaaaacg acagcagcct
     3901 gaagtactac cgctcgattc gcgagcgcta tggctcggac gatttcctga tcgtcaccta
     3961 tacgccggac ggcgagttgt tcagccagcc ggtgctcgac gacatcgcca gcctgcgcga
     4021 cgcgctggcc gcgctggagc aggtcgactc ggtggtcagc ctgctcgacg tgccgctggt
     4081 cgacagtccg ccgatgacgc tggccgagat ttcgcgcggc gtgcgcacgc tgcagagcga
     4141 cgatgccgac gtacagctcg ccgaacggga aatgcgctcc agcgcgttgt acgaaaacct
     4201 gctggtcaac agcagcggcg ataccacggc gatgcaggtc aacctggtag tcaaccgcaa
     4261 gttactcgac ctgatcaaga gccgcgacct gctctacgaa gaagccggac accatccgca
     4321 ggcgcaacaa cgtatcgagg aactgtcggc gcagatccag atcgaaaacc agctgcaaaa
     4381 ggagcgactc gagcgaacca tcgccgaggt gcgcggcatc atggacggct atcgcgaccg
     4441 ggcgacgctc ttcctcggcg gcgtgccgat gatcgcgtcc gactcgatcg ccttcattcg
     4501 cagcgacctg cgagtgttcg gcgtcggcgt ggtgctgttc atcatcgtca tcctggcatt
     4561 ttcctttggc cactcgcgct gggtagcgct gccgctggcc gtttgcctgg ccagcgcgat
     4621 gacgatgatg ggctacctcg gctgggcgca atggccggtg accgtggtgt cgtcgaactt
     4681 catctcgctg ctgctgatca tcaccttgtc cctgatcatt cacctggtgg tacgttaccg
     4741 cgaactggcg ggcctcgatg ccgcggccag ccagtacgaa ctggtacgcg ctaccgtgca
     4801 cagcaagttc gcgccgagct tttataccgc catcaccacc atggtcgcgt tcggctcact
     4861 gctggtcagc ggcattcgcc cggtaatcga tttcggctgg atgatggtca tcggcatcac
     4921 cgtatccttc gtgctcgcgt tcacgctgtt cccggccgcg ctgacactgc tgccacgcct
     4981 cgagacccgc attaaaaagg attacaccgg caaggtcacc ggggcgatcg cggatttcat
     5041 tcagcaccgc acgcgcgcga ccctgctgat cttcgtcgcc ctggtagcca tcgcgctcgg
     5101 cggtattccg cgcctgtcgg tcgaaaaccg cttcatcgat tactacaagg aggataccga
     5161 gatctaccag ggcatgacgc tgatcgacca gcggctcggc ggcaccacgc cgatggatat
     5221 catcatcgat gcgccgccgg gggcgctgcc cgacgaagac gacctgttcg gcggcaacga
     5281 tatcaccgcc aacagctact ggttcaatcg cgacggactc gaacaggccg cgctgatcca
     5341 cgactacctc gacaagctgc cggagaccgg caaagtgctg tcggtgcata ccggcatgca
     5401 attgctcgag tcgttgaacg atgaccagcc gttcaccgac ttcaagctcg cggtggtcta
     5461 caagcggctg ccggacagcg tcaagcaggc gctgatcgag ccttacctgt cggacgacgg
     5521 caaccagttg cgcttcagcc tgcgactcta cgagtcggac aggtcgctgc gccgccaggc
     5581 actgatcgac agtatttacc gcgacctgat caacgatttc gagctcgatc ccgagcaggt
     5641 caccatctcc ggcatggcgg tgctctacaa caacctgctg caaagcctgt tccgctcgca
     5701 gatcctgacc ctcggcgcgg tattcggcgc catcctgctg atgttcatcg cgctgtttcg
     5761 cgcctttcgc ctgtcgctgg ccgcgatcgt gccgaatatc atcgccgccg gcctgattct
     5821 cggcctgatg ggctggatcg gcattccgct cgacatcatg acgatcacca tcgccagcgt
     5881 gtccctcggg atcgccgtgg actcggccat ccactacgtg acacggttcc ggcgtgaggt
     5941 ccgtgcgacc ggcgacttcg aggccgccac gcgcgcggcg
//
LOCUS       DATCZV010000305         7282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_500695, whole genome shotgun sequence.
ACCESSION   DATCZV010000305 DATCZV010000000
VERSION     DATCZV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500695"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="VIW27_06800"
     CDS             complement(<1..1656)
                     /locus_tag="VIW27_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY5739418.1"
                     /translation="MIHRLQRIVQEVNRAPNVENALDLITGSLIRDLTADACSIFLAR
                     KDDPGVMVLQASSGLNPQIVGKIELRLGEGLIGTIAERAEAFNLVDAPTHKKFLLVPE
                     SGETDYPIYLGVPIIAHRKVLGVISVQRAENAFNEDEEAYLTTLAAQLATSIERAESL
                     GQFSTPQKRHVIRGVAGAPGMAIGEALVLNRGVNLESVPDRRADDVDAELKSFREAVG
                     KVRDELFEQAELMRASLPEEECALFLAYAQMLGGGSLIDDTEAAIGAGNWAPSSWRDT
                     VEQHAHVFAQMEDAYLAERANDIRDLGLRVLRKLMLEQNPYLDLPERTILVGDNVAAS
                     DLADLPIECLAGIVSAHGSSSSHVAILAHALGIPAVMGVPNLPVKQLDGAHLVVDGYN
                     GSVFINPSKAILKEYGQYLEEEAAIEQDLLGLKGQPAITLDQHRVTLLVNTGLMSDYT
                     PSLRSGAEGVGLYRTEIPFQVRDRFPSEEEQYQIYRGVLHTFRGMPVVLRTLDVGGDK
                     PLSYFPINEANPFLGWRGVRITLDHPEIFITQVRAMIRANRGIG"
     gene            complement(1696..2184)
                     /locus_tag="VIW27_06805"
     CDS             complement(1696..2184)
                     /locus_tag="VIW27_06805"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY5739419.1"
                     /translation="MIDADGYRANVGIILCNRAARVMWARRVGQDAWQFPQGGIGVDE
                     NPEDAMYRELWEETGLRPEHVTVLGATDSWLRYKLPRRLMRRSEPRCIGQKQLWYLLR
                     LESDEASFDLGVSKTPEFDHWKWVDYWYPAENVVFFKRRVYQCALAQLEARLPTTGTT
                     DD"
     gene            2277..2942
                     /locus_tag="VIW27_06810"
     CDS             2277..2942
                     /locus_tag="VIW27_06810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5739420.1"
                     /translation="MTLAIFDLDHTLIDGDSDYLWGEYMVENGIVDESEYRQRNLVFY
                     QQYQRGELDNDTYLDFALEPLTRYSLEQLHTWRADFIDHWIRPIIAAGTEELLRRHRD
                     AGHELLIISATNLFITEPIAELLAVPTILSTEPEIVDNRYTGRYLGTPTYQRGKVTVL
                     EAWLADTGHDLSGSYFYSDSINDLALLERVENPVVVHPDAELQSIAASRGWKVIDLRQ
                     RPA"
     gene            complement(2939..3505)
                     /gene="dcd"
                     /locus_tag="VIW27_06815"
     CDS             complement(2939..3505)
                     /gene="dcd"
                     /locus_tag="VIW27_06815"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY5739421.1"
                     /translation="MSIKSDRWIRRMAEEQGMIEPFEAGQIRESANGRIISYGTSSYG
                     YDVRCADEFKIFTNINSAIVDPKNFAAESFVDLNSDVCIIPPNSFALARTVEYFRIPR
                     SVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLFY
                     ESDEVCEISYKDRGGKYQGQRGVTLPRA"
     gene            complement(3585..4478)
                     /locus_tag="VIW27_06820"
     CDS             complement(3585..4478)
                     /locus_tag="VIW27_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5739422.1"
                     /translation="MSRFEELESFVAVVDYDGFGSAADKLGVAKSMVSRRVSDLEHRL
                     GVQLLQRTTRRQSLTDAGRDFYQRATQLLDDLNEAEQYVADSQCRLSGRIRLALPLGF
                     GVSLLARPIADFISSHPDISIDIDLNDRQVDLIEENIDLAIRVADLDDSTLIARRLAS
                     VHFAVCASPDYLERHGEPRHPRELAAHQVLVYSNVAPGLQWSYESGGKHIKPRVNYRL
                     SANNGAFLAAIACQGEAIVNGPLALLEGHIERGELLPILREFQRPPTGMYAVYPPGRL
                     VSRRVRALSDRLYDYFRDRDI"
     gene            4579..4986
                     /locus_tag="VIW27_06825"
     CDS             4579..4986
                     /locus_tag="VIW27_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5739423.1"
                     /translation="MNSQPLSNLVMLTGRAMLATIFIVAGISKIGAYAGTQAYMESVG
                     LPGMLLPAVIALEIGGGIAILVGFQTRIAATLLAGFTLLAAIIFHSDFSQQMQSILFM
                     KNVAIAGAFLLLIAQGPGQWAIAKGRAAAGEAS"
     gene            4983..5603
                     /locus_tag="VIW27_06830"
     CDS             4983..5603
                     /locus_tag="VIW27_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5739424.1"
                     /translation="MSARLKILRIDASANPGESASRRLGDEIIARLRQDTDIELRQRD
                     LNCEPSFIDATWIDANFTAAAERDAAQAERLRVSDALIAELQWADRILLTTPMYNFGV
                     PATLKAWIDLVCRAGVTFRYTDNGPEGLLEGRPVDIVITSGGVPLGSPVDFVSGYLQQ
                     VFTFIGLDDVNIVGAAQMNVDADAGLAAARSQIGRIETWSERREVA"
     gene            5603..6445
                     /locus_tag="VIW27_06835"
     CDS             5603..6445
                     /locus_tag="VIW27_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009801503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5739425.1"
                     /translation="MNRSALKIVNGQPATDGAGVELVRVIGQHALAELDPFLLLDAFR
                     SDDPDDYIAGFPPHPHRGFETVTYILNGRMRHKDNAGNEGVIEPGGIQWMTAGRGIVH
                     SEMPEQQDGLLEGFQLWINLPGAHKMSAPTYQEHGADEIPHESRPGAIIKVIAGQTSA
                     GTCGPVSQPLTNPLYLDVTLEASADFSEPVDAGHNAFVYVINGELELDAAAGGAARVG
                     RDQLAVLGPGDSLVVSATHSAARFLLVAGKPLGEPVARGGPFVMNTQEELMQAFADYQ
                     TGKF"
     gene            complement(6973..>7282)
                     /locus_tag="VIW27_06840"
     CDS             complement(6973..>7282)
                     /locus_tag="VIW27_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019025206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY5739426.1"
                     /translation="VLGIIENMSIHICSQCGHSEHIFGEGGGQRMSEEFGVELLGSLP
                     LDIHIREQADSGNPTVAAMSDSQVSSIYRSIARKTAARLATKARDHSAAFPNIVIENN
                     "
BASE COUNT         1418 a         2309 c         2174 g         1381 t
ORIGIN      
        1 gccgataccc cggttggcgc gaatcatcgc gcgcacctgg gtgatgaaaa tttccgggtg
       61 atcgagcgtt atgcgcacac cgcgccagcc gaggaacggg ttggcctcgt tgatcggaaa
      121 atacgacagt ggcttgtcgc cgccgacgtc gagggtgcgc agcaccaccg gcatacccct
      181 gaaggtatgc agcacgccgc gatagatctg gtattgctcc tcttcgctgg gaaaccgatc
      241 gcgcacctgg aacggaattt cggtccgata caaaccgacc ccttcggcac cgctgcgcag
      301 tgacggtgtg taatcggaca tcaggccggt attgaccagc agggttacgc gatgctgatc
      361 gagcgtgatc gcgggctggc ccttgagtcc cagcaagtct tgctcgatcg ccgcctcttc
      421 ctcgaggtac tgaccgtatt ccttgaggat cgccttgctc gggttgatga ataccgagcc
      481 gttgtagccg tcgaccacca gatgcgcgcc atcgagctgc ttgaccggca ggttcggtac
      541 acccatcacc gccgggatgc cgagcgcatg cgccaggatc gcgacgtgcg aagaactgct
      601 gccgtgcgcc gagacgatgc cggccagaca ctcgatcggc aggtcggcga gatccgaggc
      661 agcaacattg tcgccgacca ggatggttcg ctcgggaaga tcgagatagg gattctgctc
      721 gagcatcagc ttgcgcagaa cacgcaagcc gaggtcacga atgtcgttgg cacgctcggc
      781 gagataggca tcttccatct gcgcgaaaac gtgggcgtgc tgctcgacgg tatcgcgcca
      841 gctcgacggc gcccagttgc cggcaccgat cgccgcctcg gtgtcatcga tcagcgatcc
      901 gccaccgagc atctgcgcgt aggccaggaa caacgcgcac tcttcctcgg gcagcgaagc
      961 gcgcatcaat tcggcctgtt cgaacagttc gtcgcgaacc ttgcccaccg cttccctgaa
     1021 actcttaagc tcggcgtcga catcgtccgc cctgcgatcc ggcaccgact cgagattgac
     1081 gccgcggttg agcaccagcg cctcgccgat cgccatgccc ggggcgccgg cgacgcccct
     1141 gatcacatgc cgcttctgcg gagtactgaa ttgcccgagg ctctcggcgc gttcgatcga
     1201 tgtcgccagt tgcgcggcga gcgtggtcag atacgcttcc tcgtcctcgt tgaatgcgtt
     1261 ttcggcgcgc tggaccgaga tgacgccgag caccttgcgg tgggcaatga tcggcacgcc
     1321 gagatatatc gggtaatcgg tttcccccga ctccggcacc agcaggaatt tcttatgcgt
     1381 cggcgcatcg accaggttga aggcctcggc acgctcggcg atagtaccga tcaggccctc
     1441 gccgagtcgc agttcgatct tgcccacaat ctgcggattc aggccactgc tggcctgcag
     1501 caccatgacg ccggggtcgt ccttgcgcgc gagaaaaatc gaacaggcat ccgcggtcag
     1561 gtcgcgaatc aggcttccgg taatcaaatc cagcgcgttt tcgacgttcg gagcccggtt
     1621 gacctcctgc acgatacgct gcagcctgtg aatcatcgat gccacgtagt tgtccttatg
     1681 atcaccgacg aatgctcaat cgtccgtggt gcccgtggtc ggcaaacggg cttcgagctg
     1741 cgccagcgcg cactggtaaa cccggcgttt gaagaacacc acgttttcgg cgggatacca
     1801 gtaatccacc catttccagt ggtcgaactc cggcgtcttc gacaccccga gatcgaagct
     1861 ggcttcgtcg gactcgaggc gcaacaggta ccataactgt ttttggccga tacagcgcgg
     1921 ttcggagcgc cgcatcaggc gcctgggcaa tttatagcgc agccaactgt cggtcgcgcc
     1981 gagcacggtg acgtgctcgg gacgcaagcc ggtctcctcc cagagttcgc gatacatcgc
     2041 atcctcggga ttttcgtcga caccgattcc gccctgcgga aactgccagg catcctggcc
     2101 gacacgccgc gcccacatca ccctcgcagc acgattgcag aggataatgc cgacgttcgc
     2161 gcgataacca tctgcatcta tcatttcgcg gcttgccata attagtttag aattccgacc
     2221 gcccattctt acatatacgc tccaaaacca gcaagcaaca gcaaagtgat gcaaccatga
     2281 cgctcgccat cttcgacctc gaccatactc tcatcgacgg cgatagcgac tatctgtggg
     2341 gcgagtacat ggtcgaaaac ggtatcgtcg acgaaagcga gtaccggcag cgcaacctgg
     2401 tcttttacca gcaataccag cgcggggaac tcgataacga cacctacctg gatttcgctc
     2461 tcgaaccgct gactcgctac tcgctggagc aactgcacac ctggcgcgcg gacttcatcg
     2521 accactggat tcggccgatc atcgccgccg gcaccgagga gttgctgcgc cggcaccgcg
     2581 acgccggcca cgagttgctg atcatcagcg ccaccaacct gttcatcacc gagccgatcg
     2641 ctgagctgct cgcggtgccg acgatcctgt cgaccgaacc cgaaatcgtc gacaatcgct
     2701 acaccgggcg ctatctcggc accccgacct accagcgcgg caaggtgacc gtgctcgagg
     2761 catggctggc cgacaccggg cacgacctct ccggcagcta tttctacagc gactcgatca
     2821 atgatctggc cttgctcgag cgggtcgaaa atcccgtggt ggtgcacccc gacgcggaat
     2881 tgcagtcgat cgccgcaagc cgcggctgga aggtaatcga tctacgccag cgtccagcct
     2941 aggcgcgcgg cagggtgacg cctctctgcc cctggtactt gccgccgcga tccttatagg
     3001 aaatctcgca aacctcgtcg gattcgtaga acagcatctg cgcgacgccc tcgttggcgt
     3061 aaatctttgc cggcagcggc gtggtgttgg aaaattcgag cgtcacgtgt ccttcccact
     3121 cgggttccag cggcgtcacg ttgacgatga tgccgcagcg tgcgtaggtc gacttgccga
     3181 gacagatagt cagcacgctg cgcggaatgc gaaaatactc gacggttctc gccagcgcga
     3241 acgaattcgg cggaatgata cagacgtcgg aattcaggtc gacaaagctc tcggcggcga
     3301 aattcttcgg atcgacgatc gccgagttga tattggtgaa tatcttgaac tcgtcggcgc
     3361 agcgcacatc gtaaccgtag ctggaagtgc cgtaggaaat tattcggcca ttggcagatt
     3421 cgcgaatctg ccccgcctcg aatggttcga tcatcccctg ttcttccgcc atgcggcgga
     3481 tccatcgatc agacttgatg ctcacacgct gttcccgaaa aaaaacgcag tatatccgtt
     3541 tgcgggggcc gccgtcgatt accgccgccg cccgccggcg cggcctagat atcgcggtcc
     3601 cgaaagtaat cgtagagccg gtcgctgagt gccctgacgc gtctcgacac caggcgtccg
     3661 ggcggataga cggcgtacat gccggttggc gggcgttgga attcgcgcag gatcggcagc
     3721 aactcgccgc gctcgatgtg gccctcgagc agcgccagcg gcccgttgac gatggcctcg
     3781 ccctgacagg cgatggcggc cagaaaggcg ccgttattcg cgctcaggcg atagttcacg
     3841 cgcggcttga tatgcttgcc accggattcg tatgaccact gcaggccggg cgcgacattg
     3901 ctgtatacca gcacctggtg ggccgcgagc tccctgggat gccgcggttc accgtgtcgc
     3961 tcgaggtagt ccgggctcgc gcaaacggcg aaatgcacgc tcgccagccg gcgcgcgatc
     4021 agcgtcgaat catctagatc ggcgacccgg atcgcgaggt cgatgttttc ctcgatcagg
     4081 tcgacctggc gatcgttgag atcgatatcg atgctgatat ccgggtgcga cgagatgaaa
     4141 tcggcgatcg gccgggcgag caggctgacg ccgaaaccga gcggcagcgc cagccgaatc
     4201 cggcccgaca gccggcactg cgaatcggcg acatactgct cggcctcgtt gagatcgtcg
     4261 agcaactggg tcgcgcgttg ataaaaatcc cggccggcat cggtcaacga ctggcggcgc
     4321 gtggttcgct gcaacaattg cacccccagg cgatgctcga gatcggagac ccgccggctg
     4381 accatcgact tggcgacacc gagtttgtcg gcggcactgc cgaagccgtc gtaatcgacg
     4441 acggcgacga aagactcgag ttcctcgaaa cggctcatat tgttcctatt ttaacaacaa
     4501 taatttcaca aatagcttat ttattataat ttaagaacaa caataatagc cctgtcaatc
     4561 aaccacaggg tatcagccat gaattctcaa ccgttatcca atctcgtcat gctgacgggc
     4621 cgcgcgatgc tggcgacgat tttcatcgtc gccggcatta gcaagatcgg cgcctacgcg
     4681 ggcactcagg cctacatgga atccgtgggc ctgccgggca tgctgctgcc ggcggtgatc
     4741 gcgctcgaaa tcggcggcgg catcgccatc ctggttggtt tccagacccg catcgcggcc
     4801 acgctgctgg ctgggttcac cctgctcgcg gcaattatct ttcacagcga tttcagccag
     4861 cagatgcaat ccatcctgtt catgaagaac gtggccatcg ccggcgcctt cctgttgctg
     4921 attgcgcaag ggccagggca atgggctatc gccaagggcc gcgctgcggc gggagaagcg
     4981 tcatgagcgc acgcctgaaa atattgcgca tcgacgccag cgccaacccg ggcgaatcgg
     5041 ccagccgccg cctcggcgac gaaatcatcg cgcgcctgcg gcaggacacc gatatcgagt
     5101 tacgccagcg cgacctgaac tgcgagccat cgttcatcga cgccacgtgg atcgacgcca
     5161 atttcaccgc ggcagccgaa cgcgacgcgg cccaggccga acgcctgcga gtttccgacg
     5221 cactgatcgc cgagctgcaa tgggccgacc gcatcctgct gaccaccccg atgtacaact
     5281 tcggcgtccc ggcaacgctg aaggcctgga tcgatctggt gtgccgcgcg ggagtgactt
     5341 tccgttacac cgacaacggc cccgagggac tgctcgaagg gcggccggtc gacatcgtca
     5401 tcaccagcgg tggcgtaccg ctgggaagcc cggtggattt cgtcagcggc tatctgcagc
     5461 aggtgtttac cttcatcggc ctcgacgatg ttaatattgt cggcgccgcg cagatgaacg
     5521 tcgacgccga cgccggcctg gccgcggcgc gctcgcaaat cgggcgcatc gaaacctgga
     5581 gcgagcggcg ggaggtcgcc tgatgaatcg cagtgccctg aaaatcgtca acggacagcc
     5641 ggccaccgat ggcgccggag tggaactggt aagagtcatc ggccagcatg cgctggccga
     5701 actcgatccg ttcctgctgc tcgatgcatt ccgctccgat gatcccgacg actatatcgc
     5761 cggttttccg ccccatccgc accgcggctt cgaaaccgtc acctatatcc tcaacgggcg
     5821 tatgcggcac aaggacaatg ccggcaacga gggcgtcatc gaacccggcg gtattcagtg
     5881 gatgaccgcc ggccgcggca tcgtgcactc ggagatgccg gaacagcagg atggactgct
     5941 ggaaggcttt caactgtgga tcaacctgcc cggcgcacac aagatgagtg ccccgaccta
     6001 ccaggagcac ggcgccgacg agataccgca cgaatcgagg cccggcgcga tcatcaaagt
     6061 cattgccggc cagacctcgg ccggcacctg cgggccggtc agccagccgc tgacaaatcc
     6121 gctttacctc gatgtaactc tcgaagcgag tgccgatttc agcgaaccgg tcgacgccgg
     6181 gcataacgcg ttcgtctacg tcatcaacgg cgaactcgag ctcgatgcgg cggccggcgg
     6241 cgcggctcgc gtcggccgcg accagctcgc ggtactcggc cccggcgact cgctggttgt
     6301 cagcgccacc cattcggctg cccgctttct gctcgtcgcc ggcaagccgc tcggtgaacc
     6361 ggtggcgcgc ggcggcccgt tcgtcatgaa tacgcaggag gaattgatgc aggcgttcgc
     6421 ggattatcag accggcaagt tctgacactt tacgcgtcat gcccgcgcag gcgggcatct
     6481 tctgcgcagc catttctagc tccccgtttt cgcgaggatg actgcaaact gtatcagtct
     6541 tacctcgagt cattcgcgca caggcggagc gccgcccgcg accgaccgct gcgcggtggg
     6601 aatcttgcac agacccggaa caagctcgta tcggcaaatc actgctgatg tgggtggcga
     6661 tcaccgccag cgcggcgtga agatccccgc tggcgcgggg atgcctttta ggggcaaatc
     6721 cagcatcggc taagattcgc tccttgcgaa tgcgcgaggg tcctgtatcg cgtcacctcg
     6781 ggagaccccg gatagccgct gcgcggcttt cgagatgact ttgaggggca aatccggcac
     6841 ggatgagaac cggtccatgc gaatgcgcga ggtgcttgta ccgtttcaac ctcggaagat
     6901 cccggataag cgctgcgcgc tttccgggat gactttgagg cgaaaatcca gtatcggatt
     6961 ttcgcctcaa agtcagttgt tctcaatgac gatattcggg aatgccgcgg agtgatcgcg
     7021 tgctttggtc gccagccgcg ccgcggtctt gcgcgcgata ctgcggtaga tcgagctgac
     7081 ctggctgtcg ctcatcgccg ccacggtcgg gttgccgctg tcggcttgct cgcgaatgtg
     7141 aatatcgagc ggcagtgagc ccagcagttc tacgccgaat tcctccgaca tgcgctgacc
     7201 gccgccctcg ccgaagatgt gctcgctgtg gccgcactgg ctgcagatat gaatgctcat
     7261 gttctcgatg atgccgagta cc
//
LOCUS       DATCZV010000306         5167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_606867, whole genome shotgun sequence.
ACCESSION   DATCZV010000306 DATCZV010000000
VERSION     DATCZV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606867"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VIW27_06845"
     CDS             complement(<1..479)
                     /locus_tag="VIW27_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008032092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY5739427.1"
                     /translation="MVRKPAPSVIDGLRAVDTGVPDYALMCSHHREYVEALRAAGAEV
                     TELAPLDAYPDACFVEDTAICLPEGSVLMRPGAPSRAGEVDAIAPVLHELTATVRRVE
                     DGYIEGGDILICDREILVGRSARTDADGIAALQRLVADWGYPVREVETPADVLHFKT"
     gene            869..2110
                     /locus_tag="VIW27_06850"
     CDS             869..2110
                     /locus_tag="VIW27_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5739428.1"
                     /translation="MFTRAQLKFILPVLILLAAAGLYQALLGSKIERSKPVLSEKVWQ
                     IEVIEARRQTLSPSITLYGRIESPEALKAAAPGGGVVERVLVRDGDRVGSGQMLVKMD
                     RRDFEAALLQAQADLRDIENQIAELRIRHRANLSRLETERELMALAAAEVARLETLKQ
                     RQLSADTQLNEAHSELGRSELEVMSRQLEVDSFDVKLQILQARHDRGKAQLDQARLAM
                     DRSSVTAPFDAIVSEVAVSAGDRVLLGQLLVSLFPVAGLEIRAHLPTSYIASVQRALG
                     ADQRLEASVAGQPGLGRFSVVRLAGEAEATGIDLFFAIDLPPDQLRPGELLPLIMDLP
                     AVADVYAVPYQAIYGNSRIYRVVDERLEAVDVRTIGQARDASGEVRVLIRSDTILPGD
                     RIAVTHLPNAVSGLKVKTGDG"
     gene            2122..>5167
                     /locus_tag="VIW27_06855"
     CDS             2122..>5167
                     /locus_tag="VIW27_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5739429.1"
                     /translation="MQTSLLGIFARHRVAANLLMAMMILSGVIALQKLNVQFFPNFEL
                     DRISVRTVWSGANAEDIENGITIPLEQRLRSVDRLKEITSTSASGVSSISLEFDEGSD
                     MILALDEVKKQVDDFVNLPEDAETPRVSQTLRYESVARLLISGPTQIDELRQLARRFE
                     RELLAAGIDKVDIEGLPDEQIEIEIAAEQVRRLGLGLDQLAAHIDAESRDLPAGLIGE
                     EDGAREIRSLGQRRTPQDFADIIIVSEADKRIDLGDIASIRRGHQRDSPTISVDGKKA
                     VVMVLRRNETGDSLESADILHRWIEQTRPQLPPNIKIEVFNERWQLIKDRIMLLVKNG
                     LGGLALVLIILYLFLSTRVAFWVAVGIPVSFMATLAALYLAGGSINMISLFALIMALG
                     IIVDDAIVVGEDAQAHFDMGEDPLMASEGGARRMLAPVTASSLTTIAAFLPLMLVGGH
                     IGNIMSAIPLVIVCVIIASLIESFLILPGHLRHAFVQRGHRQTRLRQRLNRGFEWFRD
                     GWFRSLVRLAIEFRWTTIAAALGFLIMTAGLFFGGKTGWQFFPSPESTTVYANARFVS
                     GTSAVIVDRFLDQVEAELRNTIADIDETVVSTYYRMHASSTGRGAAITGERAGSLYVE
                     LLNSDQRSIDNRSFIDNWRSRIEPPPGIESFQISSRRVGPSRRDLTLRLTGSTPHNLK
                     NAALDLAQTLETIPGVSAIDDDLPYGREQLIYQLNPAGEAAGLSITDIGRQLRAAYDG
                     IVLQRFQNEADEVEVRLQLPDSERRRLDSLPNLPIRLPDGEFVALETVADWRSSQGFE
                     TLRHFNGRLSVDVSADVNPALNNIAEIVDDLNEFTLPSLASRYAIQYSIEGRSASQRE
                     TFADMRIGAMIALVLMYLILAWVFSSYGWPILVMLVIPLGLTGAVLGHWLMGMNMTIL
                     SVFGMFGLSGIVVNDSIILVIFYRHIREKGVAIYEALQEAACQRLRAVLLTSLTTIAG
                     LTPLLFETSRQAQFLIPMAVSIAFGLAFSTMLILLVIPAMLSI"
BASE COUNT          936 a         1632 c         1612 g          987 t
ORIGIN      
        1 tcggtcttga aatgcagtac gtcggcggga gtctcgactt cgcgcaccgg gtagccccag
       61 tcggccacca gacgctgcag tgcggcgatg ccatcggcat cggtacgcgc cgagcggccg
      121 accaggattt cgcgatcgca gatcaggata tcgccgccct cgatatagcc gtcttcgacg
      181 cgccgcacgg ttgcggtcag ttcgtgcagc acgggcgcaa tcgcatcgac ttcgccggcg
      241 cgcgagggcg cgccgggtcg catcagcacg ctcccctcgg gcaggcaaat ggcggtgtcc
      301 tcgacgaaac aggcgtcggg gtaggcatcg agcggcgcca gctcggtgac ctcggcgccg
      361 gcagcgcgca gtgcctcgac atactcccgg tgatgcgagc acatcagggc ataatccggg
      421 actccggtat cgaccgcgcg caggccgtcg atcaccgagg gcgcgggttt acgtaccacg
      481 gcacgagcga actgataaca gctatagtgc ggcaatcgaa ccgcctcccg aaaatccgca
      541 agcgtattac ctgggccggt aaaagaaaag ccccgctgct cgacataatc ctgttgccag
      601 gcgacagcgt atacagggat aactactgtc gacaacggcg tgcatgaaaa accaggcccg
      661 caaccggtgt tccggtctca atggtcgtat ccatctcgag gagatcgctg cccgttggcg
      721 cctgcatcgg gatcaacgat ccggttttga ttgcaaacgg ctgttcgaga gccttgttcc
      781 gggcccttta gcggtttacg aagttagctt gacgctgcga cggtagtgct ttacaacgat
      841 ctccagtcca atttccgcac gactatcgat gttcaccagg gcgcagctta aattcatcct
      901 gccggtgctg attttgctcg ccgccgcggg cctttatcag gctctgctgg ggagcaagat
      961 cgagcgcagc aaaccggtgc tgagcgagaa ggtctggcag atcgaggtta tcgaagcccg
     1021 ccggcaaacc ctgtcgccca gtatcaccct gtacggccgc atcgagtcgc cggaggcgct
     1081 caaggcggcc gctcccggcg gcggcgtggt ggagcgggtc ctggtccgcg atggcgaccg
     1141 tgtcggcagc gggcaaatgc tggtgaaaat ggatcggcgc gatttcgagg cggcactgct
     1201 gcaggcgcag gcggatttgc gggatatcga aaaccagatc gcggagctgc gcattcggca
     1261 ccgagccaat ctgtccaggc tcgaaaccga gcgtgaactg atggcgctgg ctgccgccga
     1321 ggtagcccgc ctggaaaccc tcaagcagcg ccagctgagt gccgataccc agctcaacga
     1381 agcgcacagc gaattgggcc gcagcgagct cgaagtcatg tcacggcagc tcgaggtcga
     1441 cagttttgac gtcaagctgc agattctgca agcccggcac gaccgtggca aggcgcagct
     1501 cgaccaggcc aggctggcga tggaccgctc gtcggtaacc gccccgttcg atgccatcgt
     1561 cagcgaggta gcggtctccg ccggcgaccg cgtgctgctg ggacagttgc tggtttcgct
     1621 gttcccggtg gcgggactcg agatccgcgc tcacctgccc accagttata tcgcctcggt
     1681 acagcgcgca ctcggcgcgg accagaggct cgaggccagc gtggccgggc agccgggtct
     1741 gggccgcttc tccgtggtgc gccttgccgg tgaagccgag gcgaccggta tcgacctgtt
     1801 cttcgcgatc gatctgcctc cggatcaatt acggccgggg gaattattgc cgctaatcat
     1861 ggatttgccg gccgtggccg acgtgtacgc cgttccctac caggcgatct acggcaattc
     1921 gcggatctac cgcgtcgtcg atgagcgcct cgaggccgtg gacgtgcgca cgatcgggca
     1981 agcgcgcgac gccagcggcg aggtgcgagt gttgatccgc agcgatacga tactgcccgg
     2041 cgatcgaatc gccgtcaccc atttgcccaa tgcggtctcc ggactcaagg tgaaaaccgg
     2101 tgatggctag gcggaagatg catgcagact agcctgctcg gtatttttgc gcgccaccgg
     2161 gtcgctgcca acctgctgat ggcgatgatg atcctgtcgg gcgtgatcgc gctgcaaaag
     2221 ctcaacgtgc agttcttccc caatttcgaa ctcgaccgaa tatccgtgcg cacggtctgg
     2281 agcggcgcca acgccgagga tatcgaaaac gggatcacga ttccgctcga gcagaggctg
     2341 cgcagcgtcg atcggctcaa ggaaataacc tcgacttcgg ccagcggcgt gtcgagtatc
     2401 tcgctcgagt tcgacgaggg tagcgacatg atactggccc tcgacgaagt caaaaagcag
     2461 gtcgacgact tcgtcaattt gccggaagac gccgagactc cgcgcgtctc gcaaaccctg
     2521 cgttacgaat cggtcgcccg cctgctgatc agcggcccga cccagatcga cgaattgcgt
     2581 cagctggcgc gccgcttcga gcgcgaactg ctcgccgcgg gtatcgacaa ggtcgatatc
     2641 gaaggcttgc ccgacgaaca gatcgaaatc gagatcgccg ccgaacaggt ccggcggctc
     2701 ggcctcggcc tcgatcaact cgccgcgcat atcgacgccg aaagccggga tctgccggcc
     2761 ggcctgatcg gcgaagagga tggtgcacgc gagattcgca gcctcggcca gcggcgcacc
     2821 ccgcaggact tcgccgatat catcattgtc agcgaagctg acaagcgcat cgatctcggt
     2881 gatatcgcca gcatcaggcg tggtcaccag cgcgattcac cgaccatcag cgtcgacggt
     2941 aaaaaggctg tggtcatggt cctgcggcgc aacgaaaccg gtgactcgct ggaatccgcc
     3001 gatatcctgc atcgctggat cgagcaaacc cgtccgcagc tgccgcccaa tatcaagatc
     3061 gaggtgttca acgagcgctg gcaactgatc aaggaccgca tcatgctgct ggtcaagaac
     3121 ggtctcggcg gcctcgcgct ggtactgatc atcctctacc tgtttctgtc gacccgggtg
     3181 gctttctggg tcgcggtcgg gattccggta tcgttcatgg cgacgctggc ggcgctctat
     3241 ctcgctggcg gctcgatcaa catgatcagt ctgttcgcgc tgatcatggc gctcggcatc
     3301 atcgtcgacg acgcgattgt cgtcggcgag gacgcgcagg cgcacttcga catgggcgag
     3361 gatccactga tggcctcgga gggcggcgcc agacgcatgc tcgcgccggt taccgcgtcg
     3421 tcgctgacga cgatcgccgc gtttctgccg ctgatgctgg tcggcgggca tatcggcaat
     3481 atcatgtcgg cgattccgct ggttatcgtc tgcgtcatca ttgcctcgct gatcgagagt
     3541 tttctgatcc tgcccgggca tctgcggcat gcattcgtcc agcgcggcca ccgccagacc
     3601 aggcttcgac agcggctcaa ccggggtttc gaatggtttc gcgacggctg gtttcgaagc
     3661 ctggtgcggc tcgcgatcga gtttcgctgg accaccatcg cggccgcgct cggcttcctg
     3721 atcatgaccg ccggcctgtt cttcggcggc aagaccggct ggcagttctt tccgtcaccc
     3781 gaatcgacca cggtgtatgc caatgcgcgt ttcgtctccg gcacctcggc cgtgatcgtc
     3841 gaccgcttcc tcgatcaggt cgaggccgaa cttcgaaata cgatcgccga tatcgacgaa
     3901 accgtggtct cgacgtatta ccggatgcac gcctcgtcga ccggccgcgg cgccgcgatc
     3961 accggcgaac gcgctggttc gctgtatgtc gagctgctca attccgatca gcgcagcatc
     4021 gacaaccgca gcttcatcga caactggcgc tcgcggatcg agcccccgcc cgggatcgaa
     4081 agcttccaga tcagttcgcg ccgcgtgggg ccgtcacggc gcgacctgac cctgcgcctc
     4141 accggctcga cgccgcataa tctcaagaac gccgcgctcg atctggcgca aacgctggag
     4201 accattccgg gagtcagcgc gatcgacgat gacctgccat acggccgtga acaattaatc
     4261 tatcagctca atccggccgg cgaggcggcc ggcctgtcga ttaccgatat cggccgccag
     4321 ttgcgcgcgg cttacgacgg tatcgtgctg cagcgcttcc agaacgaggc cgacgaagtc
     4381 gaggtgcgac tgcagttgcc ggattccgag cgccgccgtc tcgatagcct gccgaacctg
     4441 ccgatccggc tgcccgacgg tgagttcgtc gcgctcgaaa ccgtggccga ctggcgctct
     4501 tcgcagggtt tcgaaaccct gcggcatttc aatggccgcc tgtcggtcga tgtcagcgcc
     4561 gacgtcaatc cggcgctcaa taatatcgcc gaaatcgtcg acgacctgaa cgagttcacg
     4621 ctgccgtcgc tggccagccg ttacgctatc cagtacagca tcgaaggccg ctcggccagc
     4681 cagcgggaga cttttgccga catgcgcatc ggtgcgatga tcgcgctggt gctgatgtac
     4741 ctgatcctgg cctgggtgtt ctcgtcctac ggctggccga tcctggtcat gctggtgatt
     4801 ccgctgggcc tgaccggcgc agtgctggga cactggctga tgggaatgaa tatgacgatt
     4861 ctgtcggtgt tcggcatgtt tggcctgtcc ggcatcgtcg tcaacgattc gatcatcctg
     4921 gtgatattct accggcacat cagggaaaag ggcgtcgcga tttacgaagc gttgcaggaa
     4981 gccgcctgcc agcggctgcg cgcggtcttg ctgacctcgc tgacgaccat cgccggcctg
     5041 acgccgctgc tgttcgaaac ctcccgccag gcgcagttcc tgattccgat ggcggtctcg
     5101 attgccttcg gcctggcctt ctcgacgatg ctgatcctgc tggtgatccc ggcgatgttg
     5161 tcgatct
//
LOCUS       DATCZV010000307         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_183800, whole genome shotgun sequence.
ACCESSION   DATCZV010000307 DATCZV010000000
VERSION     DATCZV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183800"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VIW27_06860"
     CDS             complement(<1..958)
                     /locus_tag="VIW27_06860"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY5739430.1"
                     /translation="MIIGIPAEIHPGEKRVAASPEVVEKLIAAGFEVVVESGAGFGAH
                     FHDDVFVEVGARIARDPEEAWSSDLVIKVRPPEHNPELGRHEIDLMKEGAHLISFIWP
                     AQNEDLLKRFAERKVTALAMDSIPRISRAQKLDALSSMANIAGYRAVIEASNLFGRFL
                     AGQITAAGKVDPAKVLVIGAGVAGLAAIGTAKSLGAIVRAFDTRPEVKDQIQSMDAEF
                     LELEFEEDGTGEGGYAKVMSKEFIEAEMALFARQACEVDIIITTALIPGKPAPKLITR
                     GMVEEMRNGSVIVDLAAEQGGNCEATVPGQVVNRHGVHVVGYT"
     gene            1182..2177
                     /gene="pta"
                     /locus_tag="VIW27_06865"
     CDS             1182..2177
                     /gene="pta"
                     /locus_tag="VIW27_06865"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY5739431.1"
                     /translation="MHFLDSVLQKARDHRQTIVLAEGEDARIIEAARRAQDDGIANCI
                     LIGDPDAIHQQAAASGCQLNDISIEDPRASQYHQSYSKELWKLRKHKGMTREQAEQQA
                     LDPLCFADLMLRANHADGAIAGAIYTTGARVRSALQIVGVMPGFSSVSSFMLMLFEAE
                     HHQPKRAMIFADCALIVDPDAEQLAEIAVAAANSARQLIDDEPRVAMLSFSTDGSANH
                     PHVEKVRAATQLARRQMPATPVEGEVQLDAAIVPAIASQKISDSRIEGRANVLIFPDL
                     DAGNIGYKLTQRFGGATAIGPVLQGLNRPANDLSRGCSTDDVYHLIALTAVQSRG"
     gene            complement(2741..>3064)
                     /locus_tag="VIW27_06870"
     CDS             complement(2741..>3064)
                     /locus_tag="VIW27_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739432.1"
                     /translation="IHPEHGLWHSYRFALLGSEFAAASAETGASPCLACAGQPCLDTC
                     PVAAFDGNSYDAARCAEHLRQTPAAECHAHGCLARLACPLASRLRYRPEQGAFHLRAF
                     LDAHP"
BASE COUNT          549 a          994 c          984 g          537 t
ORIGIN      
        1 cggtatagcc gaccacgtgc acgccgtggc gattgaccac ctggcccggc accgtggctt
       61 cgcagttacc gccctgctcg gcggccaggt cgacgatcac gctgccgttg cgcatctcct
      121 cgaccatgcc gcgggtgatc agcttggggg ccggcttgcc ggggatcagc gcggtggtga
      181 tgatgatatc gacctcgcag gcctggcgcg cgaacagcgc catctcggcc tcgatgaatt
      241 ccttgctcat gaccttggcg tagccaccct cgccggttcc gtcctcctca aactcgagtt
      301 cgaggaattc ggcatccatg ctctggatct ggtccttgac ctccggccgg gtgtcgaaag
      361 cacgcacgat cgctcccagt gacttggcgg tgccgatcgc ggcgagcccg gcgacgccgg
      421 cgccgatcac cagcaccttc gccggatcga ccttgccggc ggcggtaatc tgtccggcaa
      481 ggaagcggcc gaacagattc gacgcctcga tcaccgcgcg gtagccggcg atattggcca
      541 tcgagctcag cgcgtcgagt ttctgcgcgc gcgaaatgcg cggaatgctg tccatcgcca
      601 gcgcggtcac cttgcgctcg gcgaaacgct tgagcaggtc ctcgttctgc gccggccaga
      661 tgaaactgat caggtgggcg ccttctttca tcaggtcgat ttcgtggcgg ccgagttcgg
      721 gattgtgttc gggcggccgc accttgatga ccaggtcgct gctccaggct tcctcgggat
      781 cgcgcgcgat ccgcgcgccc acttccacga agacatcgtc gtggaagtga gcaccgaaac
      841 cggcacccga ctcgaccacc acttcgaagc ccgcggcgat cagtttttcg accacctcgg
      901 gtgaagcggc gacgcgcttt tcccccgggt gaatttctgc gggaattccg ataatcatag
      961 gttattgttc ccccttggta acgtgcctcg aacattcgac cgcgacacga gacgggccaa
     1021 tatacgttat tcctggtata ttgtatacca gacttataaa ccaccgccgg gactggtgcc
     1081 gacaccaatt aattcgctct ggcgtggaca cctggacact atgcgggcat cattctgcat
     1141 caattcgcct tcccggaaca ttcgaatttt cgtatccctg catgcatttt cttgattcag
     1201 tcctgcaaaa agcccgtgat caccggcaaa ctatcgtgct cgccgagggc gaggacgcgc
     1261 gcatcatcga agccgcgcga cgcgcacagg acgacgggat tgccaactgc atactgatcg
     1321 gcgatcccga cgccattcac cagcaggccg cggcgtcggg ctgccagcta aacgacatta
     1381 gtatcgaaga cccacgggct tcacaatacc accaaagtta tagtaaggag ctgtggaagc
     1441 tgcgcaagca caagggcatg acccgtgaac aggctgaaca acaagcgctc gacccgctgt
     1501 gtttcgccga cctgatgctg cgcgccaacc acgccgacgg cgccatcgct ggcgcgattt
     1561 acaccaccgg cgcgcgcgtg cgttcggcgc tgcagatcgt cggcgtgatg cccggtttca
     1621 gctcggtgtc gagtttcatg ctgatgctgt tcgaagccga gcaccatcag ccgaagcgcg
     1681 cgatgatatt cgcggactgc gcgctgatcg tcgatcccga tgccgagcag ctggcggaaa
     1741 tcgcggtcgc cgcggccaat tcggcccgcc agctgatcga tgacgagccg cgggtggcga
     1801 tgctgtcgtt ttccaccgac ggcagcgcca accatccgca tgtcgaaaaa gtgcgtgcgg
     1861 ccacgcaact ggcgcgcaga cagatgccgg cgacgccggt cgaaggcgag gtacagctcg
     1921 atgccgcgat cgtgccggcg atcgccagcc aaaagatcag cgactcgcgc atcgagggcc
     1981 gcgccaacgt attgatattc cccgacctcg atgccggcaa catcggttac aagctgaccc
     2041 agcgcttcgg cggcgccacc gccattggcc cggtactgca gggcctcaac cgtcccgcca
     2101 acgacctgtc gcgcggctgc agcaccgacg acgtctacca cctgattgca ctgaccgccg
     2161 tccaatcccg cggctgagtg gcggatcctg atggatgccg gctcgggggc cggcatgacg
     2221 aggcacgggg gcggcgccgg cgccatcggt gccccgcggc cggcttgtgc ggtcttgcgg
     2281 agtaacgtca ttgtggcccc aggctgatgt atcggaccgc gagatccagc aaatgccgaa
     2341 tgcagtggcg gatcctgatg gatgccggct cgggggccgg catgacgagg cacgggggcg
     2401 gcgccggcgc catcggtgcc ccgcggccgg cttgcgcggt cttgccgagt aacgtcattg
     2461 tggccccagg ctgatgtatc ggaccgcgag atccagcaaa tgccgaatgc agtggcggat
     2521 cctgatggat gccggctcgg gggccggcat gacgaggcac ggggacgtcg ccgcctccgt
     2581 cggtgccccg gggccggctt tcgcagtctt ttggggtacc gtcattgcgg cgccgcccgc
     2641 ggcccgcccg agccgcaatc ttcaatatcc ggattaccca agctgctcac ttgcgcggtc
     2701 ctgtctccag cggagacaag atcagggaac ggctgcgaac tcaggggtgg gcgtcgagga
     2761 aagcacgcag gtgaaaagcg ccctgctcgg gccggtaacg taagcgcgag gccagcgggc
     2821 aggcgagccg tgccaggcag ccgtgggcat ggcattcggc ggccggcgtt tgccgcaggt
     2881 gttcggcgca acgcgcggcg tcatagctgt tgccgtcgaa agccgccacc gggcaggtat
     2941 ccagacacgg ctggccggcg caggcgaggc acggcgatgc gcccgtctcg gcggacgcgg
     3001 cggcaaactc cgaaccgagc aacgcgaagc ggtaggaatg ccagaggccg tgctcggggt
     3061 ggat
//
LOCUS       DATCZV010000308         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_555097, whole genome shotgun sequence.
ACCESSION   DATCZV010000308 DATCZV010000000
VERSION     DATCZV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555097"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VIW27_06875"
     CDS             complement(<1..683)
                     /locus_tag="VIW27_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5739433.1"
                     /translation="MARKLSMKDAINEALDQEMSRDPTVIVMGEDIVGGSGSPGENDA
                     WGGVLGVTKGLYAKHGDRLMDTPLSESAYIGAAIGASTCGLRPVAELMFLDFMGVCFD
                     QIFNQAAKFRYMFGGKAETPVTIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSN
                     AYDAKGLLIQSIRDNDPVIFCEHKNLYAHETDVPEESYTIPFGEANITREGGDVTIVG
                     YGLMVHRAS"
     gene            complement(721..1701)
                     /locus_tag="VIW27_06880"
     CDS             complement(721..1701)
                     /locus_tag="VIW27_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017014461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5739434.1"
                     /translation="MNDVFPLGKEDLLRAYRTMKTIREFEERLHVDFGRGDIPGFVHL
                     YAGEEAAATGIMMHLSDQDRIASTHRGHGHCIAKGVDINEMMGEIYGKSSGSCRGKGG
                     SMHIADLSKGMMGANGILGAGSPLACGAALAAQKLGHDGIGMSFTGDGASNQGTFLES
                     LNLAAIWNLPMVFVVENNGYAESTSVDYATAVDSYVDRAAGFGVPGVIVDGTDFFAVY
                     EAAGEVIRRAREGGGPSLLECKMVRFFGHFEGDAQTYRAEGENEDNRAHRDCLKQFRA
                     RVTEAGVIDAAELDAIDGEVLAAIDDAVKQAVAAPLPEAADLTTDVYVNY"
     gene            complement(1698..2870)
                     /locus_tag="VIW27_06885"
     CDS             complement(1698..2870)
                     /locus_tag="VIW27_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY5739435.1"
                     /translation="MAAEAGLRRGSRVRGRQSLLQAASSQRGPGMSAAQPVVGIIANP
                     VSARDIRRIVAYAGNMQITDRANIVLRLLAGLGAVGIERVLVMPENSGIRQHVMRGLQ
                     RSGNLGEGRFPAVEYLDMPISASTDDSVEAARRMRAAGVAAIIVLGGDGTHRAVVSAC
                     GEVPIAGVSTGTNNAFPRFQEPTTVGMAVGLAVSDPQLGAVAFRGNKLLEVEVAGRRE
                     IALVDVAIVAERYVGARALWHTRNFQELFVTFAESEVIGMSVIAGLLDPVDRATPEGR
                     QVRFDNSDQPACRLRAPIAPGVIETIGIESWGTLLPDTPCAVGPQQGSIAFDGERELS
                     FSTGDRPTVTLRTDAFRTIDVSACMQHGARTRLLRDDRDGAPAEINAPQSQSEEPA"
BASE COUNT          497 a          946 c          965 g          514 t
ORIGIN      
        1 tcactggcgc gatgcaccat caggccatag ccgacgatcg tcacgtcgcc accctcgcgg
       61 gtgatattgg cctcgccgaa gggaatcgtg taggactcct cggggacgtc ggtctcgtgc
      121 gcgtacaggt tcttgtgttc gcagaagatc accggatcgt tatcgcggat cgactggatc
      181 aggaggccct tggcatcgta ggcgttggag gggcagacca ccttgagacc gggaatgtgg
      241 gtaaacagcg gcgtcagcat ctggctgtgc tgggccgccg cgcgaaagcc ggcgccgacc
      301 atcgcgcgga tggtcaccgg cgtttccgcc ttgccgccga acatgtagcg gaacttggcg
      361 gcctggttga agatctggtc gaagcacacg cccatgaagt cgagaaacat cagttccgcg
      421 accgggcgca gtccgcaggt cgaggcgccg atcgcagcgc cgatataggc gctttccgac
      481 agcggggtgt ccatcaggcg gtcgccgtgc ttggcataga gccccttggt gacgccgagc
      541 acgccgcccc aggcgtcgtt ctcgcccggc gaaccgctgc cgccgacaat gtcctcgccc
      601 atcacgatga ccgtcgggtc gcgcgacatt tcctgatcca gcgcctcgtt gattgcatct
      661 ttcatactta acttgcgagc catggttatt tctcctcgtg tatttgcatg cagcgctgca
      721 tcagtagtta acgtaaacgt cggtggtcag gtcggccgct tccggcaacg gtgcggcaac
      781 ggcctgcttg acggcatcgt cgatcgcggc cagcacctcg ccgtcgatcg cgtcgagctc
      841 ggcggcatcg atgacgccgg cctcggtgac gcgggcgcgg aactgtttca ggcagtcgcg
      901 gtgcgcgcgg ttgtcctcgt tctcgccctc ggcgcggtaa gtctgcgcat cgccctcgaa
      961 atggccaaag aagcggacca tcttgcattc cagcagcgac ggaccaccac cctcgcgcgc
     1021 gcggcggatg acttcgccgg cggcctcgta gacggcgaaa aagtcggtgc cgtcgacgat
     1081 cactccgggc acaccgaaac cggcggcgcg gtcgacatag gaatcgaccg cggtggcgta
     1141 atcgaccgag gtggattcgg cgtagccgtt gttctcgacc acgaacacca tcggcagatt
     1201 ccagatcgcg gccaggttca ggctctcgag gaaggtgccc tggttggatg cgccgtcgcc
     1261 ggtaaagctc atgccgatgc cgtcatgccc gagtttctgc gccgccagcg ccgcgccaca
     1321 ggcgagtggc gagccggcgc cgagaatgcc gttggcgccc atcatgccct tcgacaggtc
     1381 ggcgatgtgc atcgagccgc ccttaccgcg gcaggagccg ctcgacttgc cgtagatctc
     1441 gcccatcatt tcgttgatgt cgacgccctt ggcgatgcag tggccgtggc cgcggtgggt
     1501 cgacgcgatg cggtcctggt cgctcagatg catcatgatg ccggtcgcgg cggcttcctc
     1561 gccggcgtag aggtgcacga aaccgggaat gtcgccacga ccgaagtcga catgcagccg
     1621 ttcctcgaat tcgcggatgg tcttcatcgt gcgataagcc ctgagcaagt cctccttgcc
     1681 taatggaaat acgtcattca tgctggttcc tccgattgtg attgtggtgc gttaatttct
     1741 gccggtgcgc cgtcgcgatc gtcccgcagc aagcgcgtgc gcgcgccatg ctgcatgcat
     1801 gccgagacgt cgatggtacg gaacgcgtcg gtacgcaggg tgaccgtcgg ccggtcgccg
     1861 gtcgaaaagg aaagttcgcg ctcgccgtca aaggcgatcg agccctgctg cgggccgacc
     1921 gcgcaaggcg tatcgggcag cagcgtgccc caggattcga tgccgatggt ttcgatcacg
     1981 ccaggcgcga tcggcgcccg caagcggcac gccggctggt ccgagttgtc gaagcgaacc
     2041 tgccgcccct cgggagtcgc gcggtcgacc gggtcgagca ggccggcgat caccgacatg
     2101 ccgatgacct cgctttcggc gaaggtgacg aacagttcct ggaaattgcg cgtgtgccac
     2161 agcgcgcgag cgccgacgta gcgctcggcg acgatggcga cgtcgaccag cgcgatttcg
     2221 cggcgcccgg caacttcgac ctcgagcagc ttgttgccgc gaaatgcgac tgcgcccaac
     2281 tgcggatcgc tgaccgcgag acccaccgcc atgccgacgg tggtcggctc ctggaagcgt
     2341 ggaaaggcat tgttggtacc ggtggagacg ccggcgatcg gtacctcgcc gcaggcggaa
     2401 accaccgcgc ggtgcgtgcc gtcaccgccg agcacgatga tcgccgcgac cccggcggcg
     2461 cgcatgcggc gcgcggcttc gacgctgtcg tcggtagagg cactgatcgg catgtcgagg
     2521 tattcgaccg cgggaaagcg cccttcgccg agattgccgg aacgctgcag gccgcgcatc
     2581 acgtgctggc gaataccgga attctcgggc attaccagca cccgctcgat accgaccgcg
     2641 ccgagtcccg ccagcaggcg cagcacgatg ttggcacggt cggtgatctg catgttgccc
     2701 gcgtaggcga cgatgcggcg aatatcgcgc gccgacaccg gattggcgat gatgccgacg
     2761 accggttgcg ccgcgctcat acccggcccc cgctgactcg atgcggcctg caaaagagat
     2821 tgtcggccgc gaacacgcga gccgcgccgc aagccggcct cggccgccat gcggttgaac
     2881 ctcgacaggt cgagcgacac cagcgagttc agcttgtcga tc
//
LOCUS       DATCZV010000309         8156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_660025, whole genome shotgun sequence.
ACCESSION   DATCZV010000309 DATCZV010000000
VERSION     DATCZV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660025"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /gene="coaD"
                     /locus_tag="VIW27_06890"
     CDS             complement(<1..401)
                     /gene="coaD"
                     /locus_tag="VIW27_06890"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY5739436.1"
                     /translation="MSTTAIYPGTYDPVTRGHIDICQRALKVFDHVVIGVADSPAKQP
                     FFDIDERFDMLETVFADNDQISVRRFSGLLIDFARECDAEVIIRGLRAISDFEYEVQL
                     AGMNRSLAPEIETVFLTAAQRYAFVSSSLVR"
     gene            complement(452..1018)
                     /gene="rsmD"
                     /locus_tag="VIW27_06895"
     CDS             complement(452..1018)
                     /gene="rsmD"
                     /locus_tag="VIW27_06895"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY5739437.1"
                     /translation="MTKSGIRIIGGQWRGRKLVVADSEGLRPTPDRIRETLFNWLAAD
                     CRGAAVLDCFAGSGALGFEALSRGARRLVALERSATALAELRELVARLAIDAVEIRGG
                     DALAAIGELHERFDLVFIDPPYARPELRRRAFEALEKADLLQPGARIYFEWPRGEDFE
                     LPSNNLDWLKRKQAGEVNYAVAEWRPSR"
     gene            1113..2078
                     /gene="ftsY"
                     /locus_tag="VIW27_06900"
     CDS             1113..2078
                     /gene="ftsY"
                     /locus_tag="VIW27_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY5739438.1"
                     /translation="MFGFKSARQKEAPEASGLFASLRQRLAKTRENLSSGLSGLLRGK
                     QRIDDDLLEQLEERLLMADVGMQATQKITARLTESLARTQAADNDGVLDALKRVMSDV
                     LQPCSVPLVIDSTRKPFVILMVGVNGAGKTTTVGKLAQQFKQQGLKVMLAAGDTFRAA
                     AVEQLQTWGERLEVPVIRQGQGADAASVIFDALQSAQARGIDVLIADTAGRLHTQASL
                     MEELAKIKRVMAKLDPHAPHETLLVLDAGTGQNALAQASNFRASIGVSGIILTKLDGT
                     ARGGMIFNVAEQLQTPIRFIGVGEAVEDLRPFDHQEFVDALLADT"
     gene            2094..2792
                     /gene="ftsE"
                     /locus_tag="VIW27_06905"
     CDS             2094..2792
                     /gene="ftsE"
                     /locus_tag="VIW27_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY5739439.1"
                     /translation="MIEFHNVTKRFEGGYEALSNVSLHLDAGQMTFITGHSGAGKSTL
                     LKIIALIEKPTRGQALLNRVNLKNVSRRRIPYIRRNIGIIFQDHQLLFDRTVFDNVAL
                     PLQIQGYRRQEIAKRTRAALDKVGLLSKERSMPITLSGGEQQRVGIARAVVHKPTLLL
                     ADEPTGNLDPILSREIMQIFVDFNRVGVTVMVASHDLALIEELDMPRMTLNRGRLILN
                     DLADIAPEGRDGLH"
     gene            2819..3772
                     /gene="ftsX"
                     /locus_tag="VIW27_06910"
     CDS             2819..3772
                     /gene="ftsX"
                     /locus_tag="VIW27_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY5739440.1"
                     /translation="MRANIRRQRPAAANRVTAWLLHHLQSMVFSFGKIYQSPATTIMT
                     VSVIGITLSLPGGFYLFLKNIDAISGDFRSTTQISLFLDLGVDEQRARALQKEIAALA
                     NVDSTQFISREQSLQEFRRDSGFGKSIDTLSSNPLPHTILVEPGEVDTFTARNLLNRL
                     QAMPEVEIAKLDTEWLERLYTIIEIARRSVTIITILFAFAVLLIIGNTIRLDIQNRYQ
                     EIVVTKLIGATDAFIRRPFLYGGVWYGFLGGIICWLIVELGYLAIAGPLERLTLLYQS
                     EMSLITFSLHDFLILILSSTLLGLAGSWIAVARHLRRIEPT"
     gene            3911..4738
                     /locus_tag="VIW27_06915"
     CDS             3911..4738
                     /locus_tag="VIW27_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5739441.1"
                     /translation="MPRKRRLLIIDSSESARSILRRELDQRAPDLEIIACGSGREAMA
                     AVERYAFDIITTGIALPDLDGYRLIEDIHQTPKNRETAIFVVSSDMQGREITTDLAEF
                     DAVTAYFDKSEGHRALVNFIVNFIGNHDDLPIRILYVDKSATSTAVTAGILEKHEIDF
                     LHFRDATAAVECLREDLDADSACSFDLLITDLMLTSKINGFDLIKTVRNDLGLDQLAL
                     PILLMTIEPGEDERTDFTAIFGAGTNDFITKPVDEDDLLQRVHTLVNIKRQNEALNP"
     gene            complement(4774..5262)
                     /locus_tag="VIW27_06920"
     CDS             complement(4774..5262)
                     /locus_tag="VIW27_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5739442.1"
                     /translation="MTIAQHKVVTIHYSVSDSESDELIDSSANGEPMTYLHGARNIIP
                     GLEQALEGKSQGDALEVTIPPAEAYGERSDDRVQQVPREAFQDMDKIEPGMAVTAQTG
                     QGQIELVVTAVDDESVTVDANHPLAGKSLKFSVTVEQVRDASEEEIAHGHVHGPGGHD
                     HG"
     gene            5659..6525
                     /gene="rpoH"
                     /locus_tag="VIW27_06925"
     CDS             5659..6525
                     /gene="rpoH"
                     /locus_tag="VIW27_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY5739443.1"
                     /translation="MSTELVATRNTLPALLGSSNLDAYIQAARRVPILSAEEEYSLAR
                     DLQDNGNIESAQALVLPHLRFVIKIANNYAGYGLPLADLIQEGTIGLMKAVKRFRPEV
                     GVRLVSFAVHWIKAEIHEYILKNWRIVKVATTKAQRKLFFKLRSQKKRLGWFKSEEID
                     HVAETLGVTRENVIEMESRLSNYDIGFDMSSDDDEDNAYLAPVNSLASNTPSPELVLE
                     AQDTEQNNMQVLGEALEELDDRSKDIVTRRWLVEPKATLHELADEYGVSAERIRQIES
                     NAFKSIKSSFAA"
     gene            6683..>8156
                     /locus_tag="VIW27_06930"
     CDS             6683..>8156
                     /locus_tag="VIW27_06930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5739444.1"
                     /translation="MEDRIKALENDLQQKREIILAQHTKLEAFFESSIDALVQMDFDG
                     HITGWNRQAEKIFGWQADDILDRKIEQTIIPERYRAAHLAAMQRYLRSGETTVMDTLV
                     EIHALHRDGHEFPVELSVSVIDSPELQEFNAYIRDISERKQAEDVIWKQANFDSLTGL
                     PNRNLFLQKLADGIRSCDRSRQSLALLYLDLDRFKDVNDTLGHAMGDLLLVEIARRLQ
                     SALRQVDTVARLGGDEFTVILGNISDQLAVQPICQSLLDELAQPFQLENEKLFLTASI
                     GVSFYPQDASEVGELQRFADQAMYAAKTEGSNRYHFFTPELQRRALRKRKMINELRDA
                     IDGRQFEILYQPIVALDGGRMHKAEALIRWLHPERGELDPDAFIPIAEDAGLIAEIGN
                     WVFYQAITEAARWREAFAEDFQISVNISPMQWLDNAAAMDQWRSHLQAAGIAGQALII
                     EITERLLIEARDRIAGRLMAFRESGIQVSIDDFGTGYASLS"
BASE COUNT         1697 a         2553 c         2339 g         1567 t
ORIGIN      
        1 tcccgcacca ggctcgagga gacgaatgcg tagcgttgcg cagcggtcag gaacacggtc
       61 tcgatttccg gtgccagcga acggttcata ccggccagct gcacctcgta ctcgaaatcc
      121 gagattgccc gcaggccgcg aatgatgacc tcggcgtcac attcgcgcgc gaagtcgatc
      181 agcagtccgg aaaatcgccg caccgagatc tggtcattgt cggcgaacac ggtttccagc
      241 atatcgaaac gttcgtcgat gtcgaaaaac ggttgcttgg cgggactgtc cgcaaccccg
      301 atgacgacat ggtcgaagac tttcagcgcc cgctgacaga tgtcgatatg accgcgcgtg
      361 acgggatcgt aggttccggg atagatggcc gtggtagaca tcgattacct caggttttcg
      421 gcgactgcgg ccggggacta aacgcccgat tttaacggct cggccgccat tcggctaccg
      481 catagttgac ctcccccgcc tgcttgcgtt tcagccagtc gagattgttc gacggcagtt
      541 cgaagtcctc gccgcgcggc cattcgaagt agattcgggc acccggctgc aacaggtcgg
      601 ccttttccag cgcttcgaaa gcacgccgac gcagctcggg ccgcgcatag ggcggatcga
      661 tgaataccag gtcgaaacgc tcgtgcagct cgccgatggc cgccagcgcg tcgccgccac
      721 gaatctcgac ggcatcgatg gcgagccgtg cgaccagctc gcgcagctcg gcgagcgccg
      781 tcgccgagcg ttccagcgcc accagacgcc gcgcgccgcg cgacagggct tcgaaaccca
      841 gtgctccact gccggcgaaa cagtcgagca ccgcggcacc gcggcaatcg gcggccagcc
      901 agttgaacag cgtttcccga attcgatcgg gcgtcggacg caggccttcg ctatcggcca
      961 cgaccagctt gcggccacgc cactggccgc cgatgattct gatgccggat ttagtcaagg
     1021 ctggtttttt ggcccaaagc ggatcatcaa gtacacgcac ggaatcgctt tctgatagca
     1081 tattgtgcct gaattattta tccggaaaaa cgatgttcgg attcaaatcc gcgcgccaga
     1141 aagaggcgcc cgaggcctcg gggctgttcg catcgctgcg ccagcgattg gcaaaaaccc
     1201 gcgaaaacct gtcttccggg ctctccggcc tgctgcgcgg caagcagcgc atcgatgacg
     1261 acctgctcga gcagctcgag gagcgcctgc tgatggccga cgtcggtatg caggcgacgc
     1321 aaaagattac cgcgcgactg accgaatcgc tggcacgcac gcaggccgcc gacaacgacg
     1381 gcgtgctcga cgcattgaaa cgcgtgatgt ccgatgtgct gcagccctgc tcggtgccgc
     1441 tggtgatcga tagtacgcgc aagccgttcg tgatcctgat ggtcggcgtc aacggcgccg
     1501 gcaagacaac caccgtcggc aagctggcgc agcagttcaa gcagcagggt ctcaaggtca
     1561 tgctggcggc cggtgacacc tttcgcgccg cggcggtgga gcagctgcag acctggggtg
     1621 aacggctcga ggttccggtc atcaggcagg gccagggagc cgatgccgcg tcggtcatct
     1681 tcgacgcgct gcaatccgcc caggcgcgcg gcatcgatgt gctgatcgcc gataccgccg
     1741 gcaggctgca cacgcaggcg agcctgatgg aagaactggc caagatcaag cgcgtgatgg
     1801 ccaagctcga cccgcacgcg ccgcacgaga cgctgctggt actcgacgcc ggtaccggcc
     1861 agaacgcgct tgcccaggcg agcaattttc gcgccagcat cggcgtgtcc ggcatcatcc
     1921 tgaccaagct cgacggcacc gcccgcggcg gcatgatctt caacgtcgcc gagcaactgc
     1981 aaaccccgat ccgctttatc ggcgtcggcg aggcggtcga ggacctgcgg ccgttcgatc
     2041 accaggagtt cgtcgatgcg ttgctggccg acacctgagg cccgcgcgca acgatgatcg
     2101 agttccacaa cgtcaccaag cgtttcgaag gcgggtacga ggcgctcagc aacgtttcgt
     2161 tgcacctcga cgccggccag atgacgttca taaccggcca ttccggggcc ggcaagagca
     2221 ccctgctgaa gatcatcgcc ctgatcgaaa agccgacccg cggccaggca ttgctcaacc
     2281 gcgtcaacct gaagaacgtg tcacgccggc gcatacccta tattcggcgc aacatcggta
     2341 tcatctttca ggaccaccag ctgctgttcg accgcacagt attcgataac gtcgcgctgc
     2401 cactgcaaat tcagggttac cggcgccagg aaatcgccaa gcgcacgcgc gccgcgctcg
     2461 acaaggtggg cctgctgagc aaggaaagat cgatgccgat cacgctctcc ggcggcgaac
     2521 agcagcgcgt cggcatcgcc cgcgcagtgg tgcacaagcc gaccctgttg ctcgccgacg
     2581 agccgaccgg taatctcgat ccgatcctgt cgcgtgaaat catgcagatc ttcgtcgatt
     2641 tcaaccgggt cggggtcacg gtgatggtgg ccagccacga cctggcgttg atcgaggaac
     2701 tcgacatgcc gcgcatgacg ctcaaccgcg gccgcctgat actcaacgac ctggccgaca
     2761 tcgccccgga agggcgcgat ggcctgcatt agtctggcgt attcgaggca accgcaagat
     2821 gcgagctaat attcgccgac aacggcctgc agccgccaac cgcgtgaccg cgtggctgct
     2881 gcaccacctg caatcgatgg tcttcagctt cggcaagatt taccagtcgc cggcaaccac
     2941 catcatgacc gtatcggtga tcggcatcac gctgtcgctg ccgggcggat tttacctgtt
     3001 tctgaaaaac atcgacgcga tctcgggaga ttttcgctca accacacaga tcagcctgtt
     3061 tctcgacctc ggcgtcgacg aacagcgtgc ccgcgcattg cagaaggaaa ttgccgcgct
     3121 ggccaacgtc gactcgacgc aattcatttc tcgcgagcaa tcgctgcagg aatttcgccg
     3181 cgattccggt ttcggcaaga gcatcgacac gctgagcagc aacccgctgc cgcataccat
     3241 cctcgtcgag ccgggggaag tcgatacctt taccgcgcgt aacctgctca accgtctgca
     3301 ggcgatgccc gaggtcgaaa tcgccaagct cgataccgag tggctggaac ggctgtacac
     3361 gatcatcgag atcgcgcgcc gctcggtgac cattatcacc atcctgttcg cattcgcggt
     3421 cttgctgatc atcggcaaca cgattcggct cgatattcag aaccgctacc aggaaatcgt
     3481 cgtcaccaag ctgatcgggg ccaccgatgc cttcatccgc cggccgttcc tgtatggcgg
     3541 ggtctggtac ggttttctcg gcggtatcat ttgctggctg atcgtcgaac tcggttacct
     3601 ggcgattgcc ggcccgctgg aacgcctgac actgctgtat caatccgaga tgtcgctgat
     3661 tacattttcg ctgcacgact ttctgattct gatattatct agtactttgc tgggtctggc
     3721 cggctcctgg atcgccgtcg cccgtcactt gcgtcgaatc gaaccgacct agtgaaccgc
     3781 ttggtatcgg gtcctgataa taactctgaa gcggacgact gaaccgatgt tttcgcaagc
     3841 agaacggatg aaaacgcacc tcaacgcgcc ggccttcgcc gtgactacgc catcttttcg
     3901 cgactgaacc atgccgcgta aacgccgact attgatcatc gattcgtcgg agtccgcgcg
     3961 cagcatactg cgtcgcgaac tggaccagcg cgctcccgac ctggaaatca ttgcctgcgg
     4021 cagcggtcgc gaagcgatgg ccgcggtgga gcgctacgcg ttcgacatca ttaccaccgg
     4081 catcgccttg ccagacctcg acggctaccg gctgatcgag gatattcacc agacgccgaa
     4141 gaaccgggag accgcgattt tcgtcgtctc cagcgatatg caggggcgtg aaatcactac
     4201 ggatctggcc gaattcgatg cggtgacggc ctacttcgac aagagcgagg ggcaccgggc
     4261 gctggtcaat ttcatcgtca atttcatcgg caatcacgac gacctgccga tcaggattct
     4321 gtacgtcgac aagagcgcga cctcaaccgc agtgaccgcc ggcatactcg aaaagcacga
     4381 gatagatttc ctgcattttc gcgacgccac cgcggccgtc gaatgcctgc gcgaggacct
     4441 cgacgccgat tcggcatgca gtttcgacct cctgatcacc gacctgatgc tgaccagcaa
     4501 gatcaacggc ttcgacctga tcaagacagt acgcaacgat ctggggctgg atcaactcgc
     4561 gctgccgatt ctgctgatga ccatcgaacc cggtgaggac gagcgcaccg acttcaccgc
     4621 gatattcggt gccgggacca acgatttcat taccaagccg gtcgacgagg acgacctgct
     4681 gcaacgggtg cacaccctgg tcaacatcaa gcgccagaac gaggcactta atccctagcc
     4741 cgcagcgacc ggaaaatacc gccgctctcg cactcagccg tgatcgtggc cgccgggtcc
     4801 gtggacgtgg ccatgcgcga tttcttcctc gctggcatcg cgcacctgct cgacggttac
     4861 gctgaacttc aacgacttgc ccgccagcgg gtgattggcg tcgacggtta ccgattcgtc
     4921 atcgaccgcg gtgaccacca gttcgatctg cccctgcccg gtttgcgcgg taaccgccat
     4981 cccgggctcg atcttgtcca tatcctgaaa agcttcgcgc ggcacctgtt gaacacgatc
     5041 atcgctacgc tcaccgtagg cctcggcggg cggaatcgtt acttcgagag cgtcaccctg
     5101 cgacttgcct tccagcgcct gttcaaggcc cggaatgatg ttgcgcgcgc cgtgcaaata
     5161 ggtcatcggc tcgccgtttg ctgaggaatc gatcagttcg tcgctttcgg aatccgaaac
     5221 gctgtaatgt atggtgacaa ccttgtgttg ggcgatggtc attagttttc ctggaatcgg
     5281 gccccttgcc cggggccggt gtattgaatt ccgggcaacc cgcggcgttc cggcggggca
     5341 acccgaaggc gttgtacgca aagacggaat ggtctggtgt ctgccgaccc gaccctacga
     5401 gtcggcaagt ataacaagtc cgcacggcaa atttcagcca tcgcgtccaa agccccgagc
     5461 agacatcgcc gatcgctcga ataactgatc caattaccgg actggtgcgt aatttcgatg
     5521 ggtttttctt tattaaccat acgcttagcg cgtatattaa tatttgctaa tatcttgaac
     5581 tttgccacca ttttatgttc taattgctgg cactcgaatg acgtgagtgc taaaaagtac
     5641 atacatcgag gtcattaaat gagcacagaa ctggtcgcca ctcgcaatac gctgcccgcg
     5701 ctcctgggca gctccaacct ggacgcctac atacaggcgg cccgcagagt gccgattttg
     5761 agcgccgagg aagagtattc gttggcccgc gatctgcagg acaacggtaa tatcgagtcc
     5821 gcccaggcgc tggtgctgcc gcacctgcgt ttcgtcatca agatcgccaa caactacgcc
     5881 ggttatggct tgccgctcgc cgacctgatc caggaaggca ccatcggcct gatgaaggcg
     5941 gtcaagcgct ttcgtcccga ggtcggcgtt cgcctggtgt cgttcgccgt ccactggatc
     6001 aaggccgaga ttcacgaata tattctcaag aactggcgta tcgtcaaggt ggctaccacc
     6061 aaggcgcaac gtaagctgtt tttcaagctg cgcagccaga agaaacgcct cggctggttc
     6121 aagtccgagg agatcgacca cgtcgccgaa accctcgggg tgacccgtga aaacgtcatc
     6181 gagatggaaa gccgtctcag caactacgac atcggtttcg acatgtcgtc cgacgacgac
     6241 gaagacaacg cctacctggc gccggtcaat tcgctggcat cgaacacgcc ttcgccggaa
     6301 ttggtactcg aggcgcagga caccgagcaa aacaacatgc aggtactcgg cgaggcactc
     6361 gaagaactcg acgatcgcag caaggacatc gtcacccgcc gctggctggt agagcccaag
     6421 gccaccctgc atgagctggc cgacgagtac ggagtgtctg cggagcgtat tcgtcagatc
     6481 gaaagcaacg ctttcaaatc gatcaaatcc tcgttcgccg cctgacggcg atcttcgaac
     6541 catcaatgcc cggcttgcag ccgggctttt ttttgccggt tacctttgcg tttcgcgact
     6601 atactcggcc cagtaactag cgaatttgcg atttatgccg gatagcgacg cccccccgga
     6661 tcgggacgac gaaacaaccc agttggaaga caggatcaag gcgctcgaga atgatttgca
     6721 gcaaaagcgg gaaatcatcc tcgcccaaca caccaagctc gaagcgtttt tcgagtcgtc
     6781 gatcgatgcc ctggtgcaaa tggatttcga cggacatatc accggctgga accgccaggc
     6841 cgaaaaaatc ttcggctggc aagccgacga tattctcgac cgcaagatcg agcagacgat
     6901 catccccgaa cgctaccgcg cggcgcacct cgcggcgatg cagcgatatc tgcgaagcgg
     6961 cgaaaccacg gtcatggata cgctggtaga gattcacgcg ctgcatcgcg acggccacga
     7021 atttccggtc gagctgtccg tgtcggtaat cgactccccg gaactgcagg aattcaacgc
     7081 ctatattcgc gacatttccg agcgcaagca agccgaggac gtgatctgga agcaggccaa
     7141 tttcgactcg ctgaccggct tgccgaaccg caacctgttc ctgcagaaac tggcggacgg
     7201 aatccgcagt tgcgaccgca gcaggcagtc actggcgctg ctctacctgg atctcgaccg
     7261 cttcaaggac gtcaacgaca cgctggggca cgccatgggt gacctgttgc tggtcgaaat
     7321 cgcacgacgg ctgcagtccg cgctgcgcca ggtcgatacg gttgcccgcc tcggcggtga
     7381 cgagtttacc gtcatactcg gcaatatcag cgaccagctt gcggtacagc cgatctgcca
     7441 gtcgctgctc gacgaactgg cgcaaccctt tcagctggag aacgaaaaac tgttcctgac
     7501 cgccagtatc ggcgtcagtt tttatcccca ggatgcgagc gaagtcggcg agttgcagcg
     7561 ctttgccgac caggccatgt atgccgccaa aaccgagggc agtaaccgct accatttttt
     7621 cacccccgaa ttgcagcggc gggcgttgcg caagcgcaag atgatcaacg agcttcgcga
     7681 cgcgattgac gggcggcaat tcgaaattct ctaccaaccg atcgtcgccc tcgacggcgg
     7741 ccgcatgcac aaggctgaag cgctgattcg ctggctgcat cccgagcgcg gagagctcga
     7801 cccggacgcc ttcataccca tcgccgagga tgctggcctg atcgccgaaa tcggcaactg
     7861 ggttttctac caggcgatca ccgaggccgc gcgctggcga gaggcattcg ccgaagactt
     7921 tcagatcagt gtcaatattt cgccgatgca atggctcgac aatgccgctg ccatggacca
     7981 gtggcgctcg catctgcagg ctgccgggat tgccggacag gcactgatca tcgagattac
     8041 cgaacgcctg ctgatcgagg cgcgcgatcg tatcgccggc cggctgatgg cctttcgcga
     8101 atccggaatt caggtctcga tcgacgattt cggtaccggc tatgcctccc tgtcct
//
LOCUS       DATCZV010000310         8427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_766213, whole genome shotgun sequence.
ACCESSION   DATCZV010000310 DATCZV010000000
VERSION     DATCZV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766213"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..990)
                     /locus_tag="VIW27_06935"
     CDS             complement(61..990)
                     /locus_tag="VIW27_06935"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5739445.1"
                     /translation="MTERVTAGGVEWPQPKNQGANMSKQKVAFIGLGVMGYPMAGHLA
                     KAGYQTTVYNRTTAKAEKWCEEYGGEFATTPREAAAGADFVFMCVGNDDDLRSVVYGD
                     NGVLAGCGDNAILVDHTTASANVAREIAAQAKLPFLDAPVSGGQAGAENGVLTVMIGG
                     EQAAFDAARPVIMSYARAAELLGGTGAGQLTKMVNQICIAGLVQGLSEGLNFAVQAGL
                     DGHKVVDVISKGAAGSWQMENRAHTMLDDQFDFGFAVDWMRKDLSICLEESAKNGAEL
                     PITRIVNDYYGEIQQMGGGRWDTSSLIRRFTQK"
     gene            complement(1027..3198)
                     /gene="uvrD"
                     /locus_tag="VIW27_06940"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1027..3198)
                     /gene="uvrD"
                     /locus_tag="VIW27_06940"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEY5739446.1"
                     /translation="MDVSHIIDPLNERQREAVSAAPGNILVLAGAGSGKTRVLTHRIA
                     WACEVDGLSPFSILAVTFTNKAASEMRARVENLLGISAAAMWVGTFHGLAHRILRLHY
                     EPAGLPQNFQILDSDDQLRMVKRVVRQLDLDEARWPPKQAQWFINHHKDEGHRPAHID
                     PGQDPIQQQMLEIYRLYEDQCRSAGVVDFAELLLRALEVIRDHDDIREHYQRRFQHIL
                     VDEFQDTNAIQYAWLRLTAGPTTPVFVVGDDDQSIYGWRGARIENIHHFEKDFADTRV
                     IRLEQNYRSTGTILKAANALIDNNRSRLGKELWTQSDDGEAILFYSAYNEKDEARFVV
                     DRIENWVEQGRSRNEVAVLYRSNAQSRQFEETLVARGVPYRVYGGLRFFERAEVKDTL
                     AYLRMISDPTADNAFERTVNHPPRGIGQKTVDEIRAHARRLSASLWNASLDLLDSDRL
                     TSRARSALQSYVDLVRRMRDELAELPLDEQIRASVETSGLDAHFRKDQSEKGQSRIEN
                     LQELIGAGKGFVFDAVIEENLTPLDAFLAHAALEAGEAQADAWEDCVQLMTMHSVKGL
                     EFPLVFLVGMEEGLFPHQRSGEEPGRMEEERRLCYVGITRAREQLVVTSAEVRRLFGS
                     ESYNMLSRFVREIPDELIEEIRPRAHFSRPGASAFESLHRERAEAATGLFVGQRVNHA
                     NFGDGVVLNLEGQGPQSRVQVNFEAAGSKWLIASLANLQPV"
     gene            complement(3285..3737)
                     /locus_tag="VIW27_06945"
     CDS             complement(3285..3737)
                     /locus_tag="VIW27_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY5739447.1"
                     /translation="MIIRIQPEDFDSVALQRELIGGRSDVGALATFIGLVRDLPDAPL
                     EQMTLEHYPGMTEKALAGIAEQACERWRILDLAVIHRVGPLKPAEQIVLVMVASRHRG
                     DAFAACEFIMDYLKTEAPFWKKETGAAGSSWVEARDSDLQAQEKWQRR"
     gene            complement(3741..4973)
                     /gene="glp"
                     /locus_tag="VIW27_06950"
     CDS             complement(3741..4973)
                     /gene="glp"
                     /locus_tag="VIW27_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5739448.1"
                     /translation="MDSCDNNPLLAYDAALERLTDGLEPLGRIVESELRAALGAVLAA
                     DVESSIDVPGCAMSAMDGYAVNTADLAVEGSTRLPLTQRIPAGAAAEPLAPGSAARIF
                     TGAPVPDGADAVIMQEQVEAGDGEIRFDARPASGSNVRPAGNDICRGTRILRRGQRLR
                     AQDIGIAASVGLQSLPVYAPLRVGIFFTGDELVEPGQDLSGGKIYDSNRYTLHGLLTA
                     LGCEIVDLGLVGDTLDATRDAMRRAASRADLVITSGGVSVGEEDHVRIALEQLGELRL
                     WRLAIKPGKPLAYGRIDGTPFLGLPGNPVSVFATFCLFVCPVIRILQGRDWQKPIALP
                     VTADFDWPRPDSRREFLRARLARNGDGRTVVQIYPNQDSGVLTSTVWADGFVEIAENA
                     TVSSGDTVDYLPFAQFLV"
     gene            complement(5025..7118)
                     /gene="recG"
                     /locus_tag="VIW27_06955"
     CDS             complement(5025..7118)
                     /gene="recG"
                     /locus_tag="VIW27_06955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY5739449.1"
                     /translation="MDHWSQQGLRYLKGVGPKVAEKLQKLGLHRQRDLLFHLPLRYED
                     RTFVTAVGSLQPGQRCQIEAEVLHTGVQFRRQGRSRRVLAAKLGDNTGVVAVRFFFFG
                     ANQQRLFEKGNWLRCFGEVRVAQGELEMIHPEVELLDRDDPAPLPSTLTPIYPTTEGL
                     HQVAIKRILEQVLDRLQSDGLDETLPHDWLERHGFPDIISAMRDLHRPDSRRASELIS
                     QHRHPAQHRFVVEELAAHRIALLQRRREIRNRETPAILPAEDLGMRLQKSLPFELTGA
                     QRRVLAELMQDFAAGRPMIRLVQGDVGSGKTVVAALACLPVVESGYQAALMAPTEILA
                     EQHYRNFADWLTPLGIGVDSLLGADKGRRRAQKLERIASGEAQVVIGTHALFQASVEF
                     DRLGFIIFDEQHRFGVDQRLALQRKTRGDEMPHQLIMTATPIPRTMAMSIYADLDYSQ
                     IDELPPGRKPVTTSIVSEQQRASLIQRVAESCAEGGQVYWVCTLVEESEALQCEAAEL
                     TYEALCRQLPQLSIGLVHGRMKSAEKDSAIGGFKNGETHILVATTVIEVGVDVPNASI
                     MIIENPERLGLAQIHQLRGRVGRGARESFCILLVRNDLSSTARRRLEIIRSTQDGFAI
                     AQQDLEIRGAGEVLGTRQTGDLSFRIADLIRDQKWFAEVEELAKLMQQPQYAAARTEL
                     MRNWIGDRQDYTVVG"
     gene            complement(7144..7527)
                     /locus_tag="VIW27_06960"
     CDS             complement(7144..7527)
                     /locus_tag="VIW27_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5739450.1"
                     /translation="MTRTIIQTDRAPQAIGTYSQAVKVDETVYISGQIPLDPATMEVV
                     AGGIEAEITRVFDNLQAVAEAAGGRLADIVKLNIYLTDLGNFPTVNEIMARYFSEPYP
                     ARAAIGIAALPKGVGVEMDAVLVLD"
     gene            complement(7780..>8427)
                     /locus_tag="VIW27_06965"
     CDS             complement(7780..>8427)
                     /locus_tag="VIW27_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5739451.1"
                     /translation="GQRLLRQSISQIRGGNANIPKSRLKKVLDQYRMADEQELLAEIG
                     LGNRNSSLVAKAMFDIEGEASVFDDSGKPLAIKGTEGMVVSFAKCCRPVPGDAIAGFV
                     SSGKGIVIHQRGCKNISERGKEDRQLSVQWSEHVEGEYLAYMRVQVANQRGVLATLAA
                     TIANMSSNIEHVNLTEKDGRISTIEFVVTVSDRIHLARIMKKLRSLPSVNRIWRR"
BASE COUNT         1492 a         2730 c         2620 g         1585 t
ORIGIN      
        1 atagatttct tctgaaatcg ccaattccga aaaaaatccg cacttttttc gaaaaaaacc
       61 ctatttttgc gtaaaacggc gtatcaggct cgaggtatcc cagcggccgc cgcccatctg
      121 ctgaatttca ccgtaatagt cgttgacgat acgcgtgatc ggtaactcgg cgccgttctt
      181 ggccgactct tcgaggcata tcgacagatc cttgcgcatc cagtcgaccg cgaaaccgaa
      241 atcgaactgg tcatcgagca tggtgtgcgc gcgattctcc atctgccagg aacccgccgc
      301 gcccttggaa atgacgtcga ccaccttgtg cccgtcgagg ccggcctgga cggcaaagtt
      361 caggccctcg gacagaccct gcacgagccc ggcgatacag atctgattga ccatcttggt
      421 gagctggccg gcgccggtac cgccgagcag ctcggcggcg cgtgcatagc tcatgatgac
      481 cggacgcgca gcgtcgaaag cggcctgctc gccgccgatc atcaccgtaa gcacaccgtt
      541 ttcggcacca gcctgaccgc cggagaccgg cgcgtcgagg aacggcagct tcgcctgcgc
      601 cgcgatttcg cgggcaacgt tggcggatgc ggtggtatgg tcgacgagga tcgcgttgtc
      661 gccgcaaccc gccagtacgc cgttgtcgcc atacaccacc gaacgcaggt cgtcgtcgtt
      721 gccaacgcac atgaatacga aatcggcgcc ggcggcggcc tcacgcgggg tagtcgcaaa
      781 ttcgcccccg tattcctcgc accatttttc cgccttggcg gtagtccggt tgtaaacagt
      841 ggtttgataa ccggccttgg cgagatgccc cgccatcgga taacccatca cgccgagccc
      901 gataaaggcg actttttgct tgctcatatt tgctccttga ttttttggtt gcggccattc
      961 tacaccgcca gcggttactc gttcagtcaa gaactccgcg cacttcgcgg agcttcctcg
     1021 atgctttcag accggctgca ggttggcgag gctggcgatc agccacttgc tgccggcagc
     1081 ctcgaaattt acctgtaccc gggactgcgg tccctggccc tcgaggttga gcaccacgcc
     1141 gtcaccgaaa ttcgcgtggt tgacgcgctg gccgacgaat agcccggtag ccgcctcggc
     1201 gcgctcccga tgcaggctct cgaacgccga tgcacccggc cggctgaaat gcgcacgcgg
     1261 gcgaatttcc tcgatcaact cgtcgggaat ttcgcgcacg aatcgcgaca gcatgttgta
     1321 gctttcgcta ccgaacaggc ggcgcacctc ggcgctggtg accaccagct gctcacgcgc
     1381 gcgggtgatg ccgacgtagc atagccgacg ttcttcttcc atgcggccgg gttcctcgcc
     1441 ggagcgctgg tgcgggaaca ggccctcttc cataccgacc aggaacacca gcggaaactc
     1501 gagccccttg accgagtgca tggtcatcag ctgcacgcag tcttcccagg catcggcctg
     1561 cgcctcgccg gcctcgagcg cggcatgcgc gagaaaagcg tccagcggcg tcaggttttc
     1621 ctcgatcacc gcgtcgaaaa cgaaaccctt gccggcaccg atcagctcct gcaggttctc
     1681 gattcgcgat tgcccctttt cgctctggtc cttgcggaaa tgcgcatcca ggccgctggt
     1741 ctcgacgctt gccctgatct gctcgtcgag cggcagttcg gcgagctcgt cgcgcatgcg
     1801 gcgcaccagg tcgacgtagg attgcagcgc gctgcgcgcc cgcgaggtca accgatccga
     1861 atccagcagg tccagcgatg cgttccacag cgacgccgac aggcggcgcg catgggcgcg
     1921 aatttcgtcg acggtctttt gcccgatgcc gcgcggcgga tgattgaccg tacgctcgaa
     1981 ggcattgtcg gcggtgggat cggaaatcat ccgcaggtag gcgagggtat ccttgacctc
     2041 ggcgcgctcg aaaaaacgca ggccgccgta aacccggtaa ggcacgccgc gcgcaaccag
     2101 ggtttcctcg aactgacggg attgcgcgtt ggagcggtac agtaccgcga cctcgttgcg
     2161 actgcggccc tgctcgaccc agttctcgat gcgatcgacg acgaaacgag cctcgtcctt
     2221 ttcgttgtag gcggagtaga acaggatcgc ttcaccgtcg tcgctttgcg tccacaactc
     2281 cttgccgagt cgcgagcgat tgttgtcgat cagcgcgttg gcggctttca ggatggtgcc
     2341 cgtcgagcgg tagttctgtt ccagccgtat cacccgtgta tcggcgaaat ccttctcgaa
     2401 atgatggatg ttctcgatgc gcgcgccgcg ccagccgtag atcgactggt cgtcgtcgcc
     2461 gaccacgaac accggtgtcg tcggacctgc ggtcagccgc agccaggcgt actggatcgc
     2521 gttggtatcc tggaactcgt cgaccaggat atgctgaaaa cgccgctggt aatgttcgcg
     2581 aatatcgtcg tggtcgcgaa tcacttccag cgcgcgcaac aacaactcgg cgaagtcgac
     2641 cacgccggcg ctgcggcact ggtcttcgta gaggcggtag atttccagca tttgctgctg
     2701 gatcggatcc tggccgggat cgatatgtgc cggccggtgg ccctcgtcct tgtggtgatt
     2761 gatgaaccat tgcgcctgtt tcggcggcca gcgcgcctcg tcgagatcca gttggcgcac
     2821 cacacgtttg accatgcgca gctggtcgtc gctgtcgaga atctggaagt tctgcggcag
     2881 tccggcaggt tcgtaatgca ggcgcaggat acgatgcgcg aggccgtgaa acgtacccac
     2941 ccacatcgcc gccgcggaaa tcccgagcag gttttcgacg cgcgcgcgca tttcgctggc
     3001 ggccttgttg gtgaaggtga ccgcgaggat gctgaatggc gacaggccgt cgacctcgca
     3061 ggcccaggca atgcgatgcg tcaacacccg cgtcttgccg ctgccggcac cggcgagcac
     3121 caggatatta ccgggcgcgg cgctgaccgc ctcgcgctgg cgctcgttca ggggatcgat
     3181 gatatgcgaa acgtccatct tcggctggct tcaggcacaa gccgccatta tatcaactca
     3241 ccgcggcaag ttgacagccg agccgtcggc gaagcggttt tttctcagcg tcgctgccat
     3301 ttttcctgtg cctggagatc gctgtcgcgc gcctcgaccc agctggagcc tgccgccccg
     3361 gtttccttct tccagaatgg tgcttcggtc ttcaggtaat ccatgatgaa ttcgcaggcc
     3421 gcgaaagcat caccgcgatg gcgactggcg accattacca gcacgatttg ctcggccggc
     3481 ttcaagggcc ctactcggtg aatgaccgcg agatcgagaa tacgccagcg ctcgcaagcc
     3541 tgttcggcga ttccggcgag cgccttctcg gtcatccccg ggtaatgttc gagcgtcatt
     3601 tgttcgagcg gcgcgtcggg caggtctcgc accaggccga taaatgtcgc cagcgcgccg
     3661 acgtcactgc gtccgccaat caactcccgc tgcagcgcaa cgctgtcaaa atcttcgggc
     3721 tggatgcgaa tgatcatggc tcaaaccaga aactgcgcga acggcaggta gtctacggta
     3781 tcgccgctgc tgacagtagc gttctccgcg atctcgacga aaccgtctgc ccacaccgtc
     3841 gaggtcagca cgcccgagtc ctggttcggg taaatctgca ccacggtgcg gccgtcgccg
     3901 ttgcgcgcca ggcgcgcgcg caaaaactcg cgccggctat cgggccgtgg ccaatcgaaa
     3961 tcggcggtta ccggcagcgc gatcggcttt tgccagtcgc gcccctgcag gatacgaatc
     4021 accgggcaga cgaacaggca aaaggtcgcg aacaccgaca ccgggttgcc gggcaggccg
     4081 agaaacggcg tgccgtcgat gcgcccgtag gcgagcggct tgccgggctt aatggccagc
     4141 cgccacaggc gcagctcgcc gagttgctcg agggcgatgc gcacgtggtc ttcctcgccg
     4201 accgagacac cgccgctggt gatcaccagg tcggctcgcg acgcggcgcg tcgcatcgcg
     4261 tcgcgggtcg cgtcgagcgt atcgccgacc aggcccaggt cgacgatttc gcaacccagc
     4321 gccgtgagca ggccgtgcag ggtatagcga ttcgagtcgt aaatcttgcc gccgctcagg
     4381 tcctgcccgg gttccaccag ctcgtcaccg gtaaaaaaga tacccactcg aagcggtgcg
     4441 tagaccggca gcgactgcaa tcccaccgag gccgcgatgc cgatatcctg ggcgcgcagc
     4501 cgctgcccgc gccgcagaat acgggtgcca cggcaaatgt cgttgcccgc cgggcgtacg
     4561 ttgctgccac tcgccggccg cgcatcgaag cgaatctcgc cgtcgccggc ctcgacctgc
     4621 tcctgcatga tcaccgcatc ggcaccatcg ggaaccggtg cgccagtaaa aatacgcgcc
     4681 gcgctgccgg gcgccagcgg ctcggccgca gcgccggccg gaatgcgctg cgttagcggc
     4741 aggcgcgtgc tgccctcgac cgccaggtcg gcggtattga cggcatagcc gtccatcgcc
     4801 gacattgcac agcccggcac atcgatcgag gattcgacgt cagcggcgag caccgcgccg
     4861 agcgccgcgc gcagctcgga ctcgacgatc cgtccaagcg gctcgaggcc atcggtcagg
     4921 cgctcgagcg cagcgtcgta ggcgagcagt gggttgttat cgcagctgtc cataaaccgg
     4981 cattagaccg caagacaggt tatccaggca accgctgacc gccactaacc gacgacggta
     5041 tagtcctggc ggtcgccgat ccagttccgc atcagctcgg tgcgggcggc ggcgtattgg
     5101 ggttgttgca tcagcttcgc cagctcctcg acctcggcaa accatttttg atcgcgaatc
     5161 aggtcggcga tgcgaaagct caggtcgccg gtctggcgcg tgccgagcac ttcacccgcg
     5221 ccgcgaatct cgagatcctg ttgtgcgatc gcgaaaccgt cctgggtgga gcgaataatc
     5281 tccagccgcc ggcgggcggt cgatgacagg tcgttcctga ccagcagaat gcagaaactc
     5341 tcgcgcgcgc cgcggccgac gcggccgcgc agctggtgaa tctgcgcgag acccagccgc
     5401 tcggggtttt cgatgatcat gatgctggcg ttgggtacgt cgacaccgac ctcgatcacc
     5461 gtggtggcaa ccaggatatg cgtttccccg ttcttgaaac cgccaatcgc gctatccttt
     5521 tcggcggact tcatgcggcc gtgcacgaga ccgatcgaca actgcggcaa ctgccggcaa
     5581 agcgcttcgt aggtcagttc ggcggcctcg cactgcaggg cctcggattc ctcgaccagc
     5641 gtgcacaccc agtagacctg gccgccttcg gcacaggatt cggcgacccg ctggatcagg
     5701 ctggcacgct gctgctcgga gacgatcgac gtggtcaccg gcttgcgtcc cggcggcaat
     5761 tcgtcgattt gcgaataatc gagatcggca tagatcgaca tcgccatggt gcgcggtatc
     5821 ggggtcgcgg tcatgatcaa ctggtgcggc atctcatcgc cgcgggtctt gcgctgcagc
     5881 gccagccgtt ggtcgacgcc gaagcggtgc tgctcgtcga agatgatgaa accgagccga
     5941 tcgaactcga cgctggcctg gaacagcgcg tgggtgccga tgacgacctg cgcctcgccg
     6001 ctggcgatgc gctcgagctt ctgtgcgcgc ctgcgaccct tgtcggcacc cagcaggctg
     6061 tcgacaccga tcccgagcgg cgtcagccag tcggcgaagt tgcggtaatg ctgctcggcg
     6121 agaatctcgg tcggtgccat cagcgccgcc tggtagccgg attcgaccac cggcaggcag
     6181 gcgagggcgg cgaccacggt cttgcccgag ccgacgtccc cctgtaccag ccgaatcatc
     6241 ggtctgccgg cggcgaagtc ctgcatcaac tcggccagca cgcgccgttg cgcgccggtc
     6301 agctcgaacg gcagcgactt ctgcagtcgc ataccgagat cctccgcggg caggatcgcc
     6361 ggtgtttcgc ggttgcgaat ctcgcgccgg cgctgtaaca gcgcgatacg gtgcgcggcc
     6421 agctcctcga ccacgaagcg atgctgcgcc gggtggcggt gctgtgagat caattcgctg
     6481 gcgcgccggc tatccggccg gtgcaggtcg cgcattgccg agatgatatc cggaaagccg
     6541 tgtcgctcta gccagtcgtg cggcagggtt tcgtcgaggc cgtcgctctg caaccggtcg
     6601 agcacctgct cgagaatgcg tttgatcgcg acctggtgca ggccttcggt ggtcgggtaa
     6661 atcggtgtca gggtgctcgg cagcggcgcc gggtcatcgc gatcgagcag ctcgacctcg
     6721 gggtgaatca tttccagctc accctgcgcc acgcgtacct cgccgaaaca gcgcagccaa
     6781 ttgccttttt cgaatagccg ctgctggttg gcgccgaaga agaaaaatct gaccgcgacc
     6841 acgccggtat tgtcgccgag cttggcggcc aatacacggc gcgagcggcc ctggcggcgg
     6901 aattgcaccc cggtgtgcag cacctcggcc tcgatctggc agcgctgtcc cggttgcagc
     6961 gatcccaccg cggtcacgaa ggtgcgatcc tcgtagcgca gcggcaggtg aaacaacagg
     7021 tcgcgctggc gatgcaagcc gagcttttgc agcttttccg cgaccttcgg gccgacgccc
     7081 ttcaggtagc gcaggccctg ttgcgaccag tgatccatac catcgccggt ttgcgacagc
     7141 gccctagtcg aggacgagca ccgcgtccat ttcgacgccg acccccttgg gcagggcggc
     7201 gataccgatc gcggcgcgcg ccggataagg ctcgctgaag tagcgagcca tgatctcgtt
     7261 gacggtcggg aagttgccga gatcggtgag atagatattg agcttgacga tgtcggcgag
     7321 ccggccgccc gcggcctcgg cgaccgcctg caggttatcg aatacacgcg taatctcggc
     7381 ttcgataccg ccggcaacga cttccatggt cgccggatcg agcggaatct gcccggaaat
     7441 ataaaccgtt tcgtctacct ttaccgcctg cgaataggta ccgatggcct gcggcgccct
     7501 gtcggtctga atgatggttc tggtcatgct ttacctcgct cgagcgaaaa tcgtttcagt
     7561 tggcgccgaa ccccgatgag acgcgtttcg agcgcccggc gcggctttgc ggcaggaatc
     7621 gtcagccgag aactctacac gacaatccgt cggttggcca tgccgcccgc ggcggcgcgc
     7681 actgcgctga cggcggagga atttcgctgc gcttattggc ctgcgacggt cgaccggcgg
     7741 cacgccaggc acgccgatag cagcggcatc agcgcacgct cagcggcgcc agatacgatt
     7801 caccgacggc agcgagcgca gctttttcat gatccgcgcg aggtgaatgc ggtcgctgac
     7861 cgtgaccacg aattcgatgg tcgaaatacg gccgtccttc tcggtcagat tgacgtgctc
     7921 gatgttggaa gacatgttgg cgatggtcgc cgccagggtc gccagcacgc cgcgctgatt
     7981 ggcgacctgc acccgcatgt aagccaggta ttcgccttcg acgtgctccg accactgcac
     8041 cgacagctgg cggtcttcct tgccgcgctc ggatatattc ttgcagccgc gctggtggat
     8101 cacgataccc ttgcccgagg atacgaaacc ggcgatcgca tccccgggta ccggccggca
     8161 gcacttggcg aacgatacca ccatgccctc ggtacccttg atcgccagcg gcttgccgga
     8221 atcgtcgaac accgaggctt cgccctcgat atcgaacatc gccttggcga ccaggctcga
     8281 attgcgattg ccgagaccga tttcggcgag caattcctgt tcgtcggcca tgcggtactg
     8341 gtcgaggact tttttcaagc gggatttggg aatgttggcg ttaccacccc tgatctggct
     8401 gatcgactgc cgcaacaggc gctgccc
//
LOCUS       DATCZV010000311         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_342476, whole genome shotgun sequence.
ACCESSION   DATCZV010000311 DATCZV010000000
VERSION     DATCZV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342476"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VIW27_06970"
     CDS             complement(<1..1304)
                     /locus_tag="VIW27_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY5739452.1"
                     /translation="MEKRPYSKSLRGKITNRTLFIGIFPVLLVGAFSWFSLTQLISSA
                     NQQLDRSRAELLDSVVGNNLSTTSARIVNQLDAFMLERISDVVVWASAPIIIQASRRA
                     ADEHTRLGLDKLSIDEVEARFDERKSLNVSPAANRYLIQQIRRSSHFGEVFFTDRFGF
                     NTALTNPTSDFVQLDENWWKTAWENGISVGEVEFDESAGIWSIDISVRIDDPGSGRSL
                     GVMKAVLGVSLIQEVADNGARDVPGGTVTVIGSSGMLLAETGTKHDKKRIMVESVNMR
                     NNNDPAIQKIFSSEARGYVISSSDVYGFAKSAGPELYRTVVSRFPGFSWTVVVQQPTS
                     IALAPIDGLATVQSGLRTSQQQIIAILVIVLIVVFVLAFFIAGLLSKTITRPLMDLRE
                     LADSVSKGDTSRTITVKSDDEIQDVAQAFERMRTSVSIIMKR"
     gene            1675..2520
                     /locus_tag="VIW27_06975"
     CDS             1675..2520
                     /locus_tag="VIW27_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006369559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY5739453.1"
                     /translation="MMISRRFIQCDVFTPTPTLGNALAVVVDGEGVSEQAMQRFAAWT
                     NLAETTFMLPPRDPAADYRLRIFTPGSEMLFAGHPTLGSCAAWLHTGGVTRQPGLARQ
                     ECAIGIVEIDIRDPGLPAFTAPPTEIRPMTDGNFDAISEALDIDPARVLRRAELDNGP
                     VWQALQLDSAAAVLALDSSRVRWPALRSIGVIGAHPPGAECDYEVRMLAPSSGMSEDP
                     ITGSLNSALGHWLQAEGLLAQPLSVAQGTCIERHGRVAITQADDGSLRIGGQTHILIE
                     GTVLL"
     gene            2530..3174
                     /locus_tag="VIW27_06980"
     CDS             2530..3174
                     /locus_tag="VIW27_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5739454.1"
                     /translation="MIELWGRANAYNVQKVLWTLRELGLEFRHHDLGSVDGELETTEF
                     LQLNPHARIPVIRDGDAVVWESNSIVRYLGARYDAGGLWPTDAWRRSLAERWMDWELA
                     TLQPDFIDLFWGFYRTPEQARDADAIADAARRCARDFEALDRQLSLTPYLGGDDFGIG
                     DIPCAVCLHRYFEMGYAVPRPRFVMQWYARLAERPAYRATVMIAFDELRGRLDF"
     gene            3265..3717
                     /locus_tag="VIW27_06985"
                     /pseudo
     CDS             3265..3717
                     /locus_tag="VIW27_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008958750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3710..4201
                     /locus_tag="VIW27_06990"
     CDS             3710..4201
                     /locus_tag="VIW27_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5739455.1"
                     /translation="MPDAAIERLDPDDPEVRRIIAAADDYYRGLYPAESNHLEPVEAL
                     KRPGMLFVGCRVDGRLAASGAAKIMTDDGTYGEIKRVFVLDEYRGLGLSRRIMDYLEA
                     RLREQGIELLRLETGIHQPEAIGLYRKLGYRERGPFGAYRVDPLSVFMEKRAAGPPAG
                     QGS"
     gene            4330..>4643
                     /locus_tag="VIW27_06995"
     CDS             4330..>4643
                     /locus_tag="VIW27_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739456.1"
                     /translation="MYKIGSIGDGFLAIMGRPFVERDAPASLRNIARLGIQQVVSLLE
                     PAEARSIGLDGERREAQAQRLVFVSYPIPDMGTPGSVHEFADLTLKLFWQIDRGVNTL
                     VH"
BASE COUNT          797 a         1461 c         1523 g          862 t
ORIGIN      
        1 atgcgcttca tgatgatcga gacgctggtg cgcatgcgtt cgaacgcctg ggccacgtcc
       61 tggatttcgt cgtccgattt caccgtgatg gttcgcgagg tatcgccctt gctgaccgag
      121 tccgccagtt cgcgcagatc catcaacggt cgggtaatgg ttttcgacag cagcccggcg
      181 atgaaaaacg ccagcacgaa gaccacgatc aacacgatca cgaggatggc gatgatttgc
      241 tgctgcgagg tcctcaggcc ggactgcacc gtggccagtc cgtcgatcgg cgccagcgca
      301 atcgaggtgg gctgttgcac gactaccgtc cagctgaatc ccgggaatcg cgacaccacg
      361 gtacggtaga gttcgggacc ggcggacttg gcgaatccat agacgtcgct ggaactgatc
      421 acatagcccc gcgcctcgct ggagaatatc ttctgaatcg ccgggtcgtt gttgttacgc
      481 atgtttaccg attcgaccat gatgcgcttc ttgtcgtgct tggtgccggt ttcggccagc
      541 agcatgccgc tgctgccgat cacggtgacg gtaccgccag gcacgtcgcg tgcgccgtta
      601 tcggcgactt cctggatcag gctgacacct agcacggctt tcattacgcc gaggctgcgt
      661 cccgagcccg gatcgtcgat gcgcaccgag atatcgatcg accagatgcc cgccgattcg
      721 tcgaactcga cctcgccgac ggagatgccg ttttcccagg cggttttcca ccagttctcg
      781 tcgagttgta cgaaatccga ggtcggattg gtgagcgcgg tattgaaacc gaagcggtcg
      841 gtgaagaata cctcaccgaa atgcgacgag cgtcgaatct gctggatcag atagcggttg
      901 gcggctggcg acacgttgag gctcttgcgc tcgtcgaaac gggcctcgac ctcgtcgatc
      961 gacaacttgt cgagcccgag gcgggtgtgt tcgtcggcgg cgcggcgcga ggcctgaatg
     1021 atgatcggcg ccgaggccca gaccaccacg tccgagatac gctcgagcat gaacgcgtcg
     1081 agctggttga cgatgcgcgc cgaggtcgtg ctcaggttgt tgccgaccac cgaatcgagc
     1141 agctccgcgc ggctgcggtc gagctgctgg ttggcgctgg aaatcagctg tgtcaggctg
     1201 aaccacgaaa aagcaccgac cagtaaaacc gggaagatac cgatgaacag ggtacgattg
     1261 gtgatttttc cgcgcaggct cttgctgtag gggcgttttt ccatcgtctt gtctcgttac
     1321 gactgtgatc gttgctggtc gggacgagcg gacggatctg gcggcgccgc gacgtcctcg
     1381 acacggtgaa ttataggttc cgcctatgat ttattattta ccgctcaaac acaatggttt
     1441 agccgattta tctccgctaa tttttgagcc tgcggcgttt tgcaacgaaa aatcaacgat
     1501 ttggcggagt tcgcggatat cgaaaccggg cgccggttgc cggccgcgag cctcgccggc
     1561 gcttggcatc gccgcgccat tgtgctctag tagcggcttg ctacaggggc tcgccgtgcg
     1621 ccatcgcgcg ccacgcgggc aattcgcgcg gctctcgtac caatgcggag gcagatgatg
     1681 atttcgcgcc gctttatcca gtgcgacgtg tttacgccga caccgaccct cggcaacgcg
     1741 ctggccgtgg tggtcgacgg ggaaggggtg tcggagcagg cgatgcagcg cttcgccgcc
     1801 tggaccaatc tcgccgagac caccttcatg ctgccgccgc gggacccggc ggctgactat
     1861 cggctgcgca tcttcacacc ggggagcgaa atgctgtttg ccggtcatcc cacgctcggc
     1921 agttgcgccg cctggctgca caccggcggg gtaacgcggc agccgggcct ggcacgccag
     1981 gaatgcgcta tcggtatcgt cgagatcgat atccgcgatc ccggtctgcc ggcctttacc
     2041 gcaccgccga ccgagatcag accaatgacg gacggcaatt tcgacgccat tagcgaggcc
     2101 ctcgacatcg atcccgcgcg ggtgttacgg cgcgccgaac tcgataatgg cccggtctgg
     2161 caggccctgc agctcgactc ggcggccgcg gtgctggcgc tggattcgtc gcgcgtccgc
     2221 tggccggcgt tgcgctcgat cggcgtcatc ggcgcgcacc cgccgggcgc cgaatgcgac
     2281 tacgaggtgc gcatgctggc gccgtccagc ggcatgagcg aggatccgat taccggctcg
     2341 cttaactctg cgctgggcca ctggctgcag gccgaaggcc tgctggcgca gccgctgagc
     2401 gttgcccagg gcacctgcat cgagcgccac gggcgggtcg cgattaccca ggccgatgac
     2461 ggctcgttac gcatcggtgg gcagacccat attctcatcg aaggcacggt cctgctgtag
     2521 cgcgccgcga tgatcgaact ctggggcagg gcgaacgcct acaatgtgca aaaggtgttg
     2581 tggacgctgc gcgagctcgg cctcgagttt cgccaccacg atctcggcag tgtcgacggc
     2641 gaactcgaaa ccacggagtt cctgcaactg aacccgcacg cgcgcattcc ggtgattcgc
     2701 gacggcgacg ccgtggtctg ggaatcgaac agcatcgtgc gttacctcgg cgcgcgctat
     2761 gacgccggcg gactgtggcc gaccgatgcc tggcggcgtt cgctggccga gcgctggatg
     2821 gactgggagc tggcgaccct gcagcccgac ttcatcgacc tgttctgggg cttttatcgc
     2881 acccccgaac aggcgcgcga cgcggacgcg atcgccgacg ccgcgcgccg ctgcgcgcgc
     2941 gatttcgagg ccctcgaccg tcagctgagc ctgacgccgt atctcggcgg cgacgacttc
     3001 ggtatcgggg atattccgtg cgcggtctgc ctgcaccgct atttcgagat gggctacgcg
     3061 gtgccgagac cgcgtttcgt gatgcagtgg tacgcgcgcc tcgccgagcg cccggcctac
     3121 cgcgcgaccg tgatgatcgc gttcgacgag ctgcgcggcc ggctcgattt ttaacccgcc
     3181 ggtgagggag gaaaccatgc gagaccagca cctgcatgcc gggcccgagc gccagccgct
     3241 tgccccgcgg cgcggcaact ccgcgaggcc cgcagcttga gcgctgtcgg ttaccgagcg
     3301 atgacgatcg acgactacga cgcggtggtc gaactatggc ggtcctgcga gggcctgagc
     3361 attcgcgacg cggattcccg cgacggcgtg cagcgctacc tggcgcgcaa tcccggtctc
     3421 agcttcgtcg ccgcgcgcgg cgcgcggatc gtcggctcga tcatggccgg gcacgacggc
     3481 aaacgcggtt acattcaaca cctcgcggtc gccgaggaag cgcgccgaca gggcgtcgcg
     3541 accagcctgg tggcgcgctg cgtcgacgcg ctccgggccg agggtatcga gaaatcgcac
     3601 gtgcacgtgc tcaaaaacaa cggcgatggc cgtgcctact ggtcaaaact cggctggcgg
     3661 cagcgcggcg aggtgatcat gtactcgttc atcaacggcg acaacgaaaa tgcctgatgc
     3721 cgctatcgag cgcctcgacc cggacgaccc ggaagtgcgg cgaatcatcg ccgccgccga
     3781 cgactattac cgcgggctct atcccgccga gagcaatcac ctggaacccg tcgaggcgct
     3841 gaagcgcccg ggcatgctgt tcgtcggctg ccgcgtcgac ggccggctgg cggcgagcgg
     3901 cgcggccaag atcatgaccg atgacggcac ctacggcgaa atcaagcgcg tgttcgtgct
     3961 cgacgaatac cgcgggctgg gattgtcgcg gcgcatcatg gattacctgg aagcgcgcct
     4021 gcgcgagcag gggatcgagc tgctgcgcct ggaaaccggt atccaccagc ccgaggccat
     4081 cgggctgtac cgcaagctcg gctaccgcga acgcgggccg ttcggcgcct accgcgtgga
     4141 tccgctgagc gtgttcatgg aaaagcgcgc agcgggaccg cccgcgggac agggatcgtg
     4201 acggtcgtcc cgcggtttta cgtcgctgct ggggttgttt tcgagccgct ggcgctggaa
     4261 cgcgataagc atttgccgat cgccgaattt atcgatttgg cgttataatt ccgcccgatg
     4321 catgcggaaa tgtacaagat cggttccatc ggcgacggct ttctcgccat catgggtcgt
     4381 ccgttcgtcg aacgcgacgc gcccgcgtcg ctgcgcaata tcgcgcgcct gggcatacag
     4441 caggtggttt cgctgctcga gcctgccgaa gcgcgcagca tcgggctcga cggcgagcgc
     4501 cgcgaggccc aggcacagcg gctggtgttc gtttcctatc cgattcccga tatgggcaca
     4561 cccggctcgg tgcacgaatt tgccgacctg acgctaaagc tgttttggca gatcgaccgg
     4621 ggcgtcaata cgctggtgca ttg
//
LOCUS       DATCZV010000312         7817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_342786, whole genome shotgun sequence.
ACCESSION   DATCZV010000312 DATCZV010000000
VERSION     DATCZV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342786"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VIW27_07000"
     CDS             <1..341
                     /locus_tag="VIW27_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEY5739457.1"
                     /translation="LLELGVEIQVSQQLVEVKAGRAIVANVYSDSLQQEIEFDTLVTI
                     TARLPEDELYRRLQQYREHFKTLRAIGDCHAPGSVAAAVYDGHSAARHLESGKDIYAP
                     LFNRELPALG"
     gene            391..2235
                     /locus_tag="VIW27_07005"
     CDS             391..2235
                     /locus_tag="VIW27_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5739458.1"
                     /translation="MAWQHRVLRVDLGAGRCDIEPLRDDWAQDYLGQRGLGSKYLVEE
                     IDPRVDPLSPDNKLIIATGPLTGTSASTGGRSSAITKGALTGAIAASNTGGLFGAELK
                     LAGYDLLILEGRAPAPVYLWIRDDEVEIRDAGGWWGRSVWETEPGLRAELGEPQAKIA
                     SIGRAGEIGVRFACIVNDMDRAYGRSGVGAVMGSKNLKAIAVRGSRGVAVRDFVAFRE
                     AVAETLAVMQPSAIRKRFTTRGTHNMMDVTHRFGALPTRNCRDVQFEGVEAINADAVK
                     VPRRSDGKPSLQGNKACFACSIACGRIATLDPSSNYANGEDPRGGDRARYRQPSGGLE
                     YENAYAFGPMCGVDDLDTLNYANFLCNEHGMDPISMGVSVAAAMELFEDGVIGVEHTG
                     GLELRFGNAAALARAVEMTAFGDGFGADIGLGAARLCAKYGRPELAMAVKGQEFPGYD
                     PRGMKGMALAYATSNRGGCHMRARPVVADLGGVTTDGKVELVKTTQDLVAAIDSSGLC
                     MFANAMFPPEHLAAMIDAACEGGWTLERLYRTGERIWNLERQFNLAAGFTAADDRLPE
                     RVVNEPARGGVAGGEVADIAAMLADYYALRGWDAHGVPLEPTLRRLGL"
     gene            2245..2496
                     /locus_tag="VIW27_07010"
     CDS             2245..2496
                     /locus_tag="VIW27_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5739459.1"
                     /translation="MAIQISLFGFGDERPAVFGGGNRLELPAPAPLSPKELLQAAGFG
                     DTEGLVLIVDDTVIPRADWQRPLIDDGTAVTVLSAFEGG"
     gene            complement(2590..3681)
                     /locus_tag="VIW27_07015"
     CDS             complement(2590..3681)
                     /locus_tag="VIW27_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739460.1"
                     /translation="MASVGKLAHLDNRRRMRLIRRCAWLILMLSCAARAAGDEPADES
                     PWLLVPTMSSDPKLGTSVGAMGGYLFRIDPESRQSMALTSISYSDSDSYVGGVFGQLY
                     FDANRQKLLVGVARGKINNDYDDFLGSGIPAQTTDQLNAEFLRYYRGITDNWYLGAQA
                     VSSNYVIGADDLLGEILDRIGLTGFDSVGAGLVAEYDSRDKIRNPGDGDYLVIHNIAY
                     SKSLGGDVSFDTYRIDYSRYREIVESHLLAWQLSGKWTDDAPIGGYASISLRGYVRGN
                     YLAPNYTHVQAEDRISLGSRWGMAVFAGIACLYDGLSDCEQSENLFASAGAGVIYTLK
                     PEAGIVIRSEIAKGESSDYVFYLRMGHPF"
     gene            complement(3755..4672)
                     /gene="speB"
                     /locus_tag="VIW27_07020"
     CDS             complement(3755..4672)
                     /gene="speB"
                     /locus_tag="VIW27_07020"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012155707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY5739461.1"
                     /translation="MTDKDFPSSYSMFSNIHGYLGLPLTRDLDAADVFVVGLPYDLGT
                     SGRSGTREGPLGIRTASAQLRWEENRWPWTFDLFERLRVADYGDLEYPFGNHEALFAS
                     VAEHYGKLLAAGKTTLTFGGDHFITLPLLRAHAKQHGKLAMIHFDAHTDTHDHPDSVY
                     FHGSMFYHAPRESIIDPERSIQVGIRTMYTASDHEFEVIDAAQANEISVDEIVARIRA
                     RVGDSPCYLTFDIDCLDPAYAPGTGTPVAGGLTSDRALKIIRGLTGINLVGMDLVEVA
                     PAYDHAQITSLAGATLGLEMLYVLAATSG"
     gene            complement(4672..6033)
                     /locus_tag="VIW27_07025"
     CDS             complement(4672..6033)
                     /locus_tag="VIW27_07025"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5739462.1"
                     /translation="MNSSGGKQLNLKIDSLTAAHPGLAQLEVLSTDLCGHFFGKRYPL
                     DKLESFVRDGLAMPASMFVLNTLGEPLEGMYYGIDDGDPDAHFSLVDSSLCVNDWGNS
                     PRAQILATTCHSDAPAWFEPRYVLQRVVDSYAERNWRPTVAFELEFYLFDGRRGEDGG
                     LRVARNPKTGREDTATVLSSTRVGDFEAVIDDIVRACELQRIDTGAISSELGPGQFEI
                     NFNHHDDTLRAADETCLFKRTVIEVAKKHGFCASFIAKPLLEQAGNGLHMHISVVDAN
                     GDNIFAAGERAHPRLRHAIGGLLETMPAAMSFWAPNVNSYRRYRGGISCAPVSLTWGD
                     ENRTVAFRVPLAQQGAWRVENRVPGADANPYLAMAVTLAAMLHGIDAALDPGPATEEA
                     VTGRDESLPLNLADALAATRASQVLDNSLGAEFVDLYCRHRAAETAAFDNYISAREYD
                     WYL"
     gene            complement(6164..6874)
                     /locus_tag="VIW27_07030"
     CDS             complement(6164..6874)
                     /locus_tag="VIW27_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5739463.1"
                     /translation="MKSKDSAALKLLMLKKSEQMKTHQWVYQLLRSNLLCGRIEPGKP
                     LTIRGLAELLHVSPMPVREALHRLACEGAVEVKNNRRVIVPLMTAEKFGELCDLRILL
                     ETHAAERALPYVKDADIEALEQIDAAIDAAIEANDADHISLENQAFHRHLYSTNPFQI
                     SVPLIESLWLQLGPFSRIAIDKLEKVYLVDRHHEAIDALKKRDGFGLKRAIEADIRDG
                     IASINTIEGIHDYFRGVA"
     gene            7067..7804
                     /locus_tag="VIW27_07035"
     CDS             7067..7804
                     /locus_tag="VIW27_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY5739464.1"
                     /translation="MKIGILEAGLLREEMASRFDPYPRMFTDFLALAHADFDYRTVSV
                     VRGEMPAGVYDCDGWLITGSRHGVYDRLDWMPPLEEFIRQLATARVPLIGVCFGHQII
                     ASALGGEVVKSEQGWGVGLHRYRVDRRHAWMHGDEAETSLYAFHQDQVITGPAEAEVF
                     LSSEFCPYAGLSYGDSIISVQAHPEFGAEYETALLEAYGGSVVPAAVAAAGLDSMRDG
                     ACADTALLANWFANFFLSRRLQAAGNG"
BASE COUNT         1363 a         2382 c         2608 g         1464 t
ORIGIN      
        1 gcctgctcga actcggtgtc gaaatccagg tttcgcaaca gctggtcgaa gtaaaagccg
       61 gcagagcgat cgtcgccaac gtgtacagcg acagcctgca gcaggaaata gaattcgata
      121 cgctggtgac gattaccgcg cgtctgccgg aggacgaact ttaccggcgt ttgcagcagt
      181 accgggagca cttcaagacg ctgcgcgcga tcggcgactg ccacgcgccc ggcagtgtgg
      241 ccgccgccgt ttacgacggt cattcggcgg cgcgtcacct cgagtccggc aaggacatct
      301 acgcgccact attcaaccgc gagcttcccg cgctgggctg aggcgtgcgg acgcggtaaa
      361 gtaggccatc gaattcgaag cggaggctgc atggcctggc aacaccgagt actgcgcgtc
      421 gatctcggcg ccggtcgctg tgatatcgag ccgctgcgcg acgactgggc gcaggattac
      481 ctcggccagc gcggcctcgg cagcaagtac ctggtcgagg agatcgatcc gcgcgtcgac
      541 ccgctatccc ccgacaacaa gctgatcatc gccaccgggc cgctgaccgg caccagcgcg
      601 tccaccggcg gccgctcgtc ggcgatcacc aagggcgcgc tgaccggcgc gatcgcggcg
      661 tcgaataccg gcggcctgtt cggtgccgag ctgaagctgg ccggctacga cctgttgatc
      721 ctcgagggtc gcgcgccggc gccggtctac ctgtggattc gcgacgacga ggtcgagatc
      781 cgcgacgccg ggggctggtg gggacgcagc gtctgggaga ccgagccggg cttgcgcgcc
      841 gaactcggcg agccgcaggc gaagattgcc agcatcggtc gcgccggcga gatcggcgtc
      901 cgcttcgcct gcatcgtcaa tgacatggac cgcgcctacg gccgctccgg tgtcggcgcg
      961 gtgatgggat cgaaaaacct caaggcgatc gcggtgcgcg gcagtcgcgg cgtggctgtg
     1021 cgcgactttg tcgcgtttcg cgaggcggtc gccgagacgc tcgcggtgat gcagccgagc
     1081 gcgattcgca agcgcttcac gacccgtggc acgcacaaca tgatggatgt cacccaccgc
     1141 ttcggcgcgc tgccgacgcg caattgccgc gacgtgcagt tcgagggcgt cgaagcgatc
     1201 aatgccgacg cggtcaaggt accgcggcgc agcgacggca agccgagcct gcagggtaac
     1261 aaggcctgct tcgcctgcag catcgcctgc gggcgcatcg ccactctcga cccgagctcg
     1321 aactatgcca acggtgaaga tccgcggggc ggcgaccggg cccgctaccg gcagccgtcc
     1381 ggcggcctcg aatacgaaaa tgcctacgcg ttcggcccga tgtgcggcgt cgacgacctg
     1441 gatacgctga attacgcgaa tttcctctgc aacgagcacg gcatggatcc gatctcgatg
     1501 ggcgtgtcgg tggccgcagc gatggaattg ttcgaagacg gcgttatcgg cgtcgagcac
     1561 accggtggcc tcgagttgcg tttcggcaac gccgcggcac tggcgcgcgc ggtcgagatg
     1621 accgcgttcg gcgacggctt cggcgccgat atcggactcg gcgccgcgcg cctgtgcgcg
     1681 aagtacggtc gcccggaact ggcgatggcg gtcaagggtc aggagttccc gggctacgat
     1741 ccgcgtggca tgaagggcat ggcactcgcc tacgcgacct ccaaccgcgg cggttgccac
     1801 atgcgtgcgc ggccggtggt cgccgatctc ggcggggtga ccaccgatgg caaggtcgaa
     1861 ctggtcaaga ccacccagga cctggtcgct gcgatcgaca gttcgggcct gtgcatgttc
     1921 gccaacgcga tgttcccgcc cgagcacctc gcggcaatga tcgatgccgc ctgcgagggc
     1981 ggctggacgc tcgagcgcct ttaccgcacc ggtgaacgca tctggaacct ggagcggcaa
     2041 ttcaatctcg ccgccggatt taccgccgcc gacgaccgcc tgcccgagcg ggtggtcaac
     2101 gaaccggcgc gcggtggcgt ggccggtggc gaggtcgccg acatcgcggc gatgctggcc
     2161 gactactacg cgttgcgcgg ctgggacgcg cacggcgtgc cgctcgagcc gaccctgcgg
     2221 cggctcggac tctagcggtc gcggatggca atccagattt cactattcgg cttcggcgac
     2281 gagcgtccgg cggtatttgg cggcggcaat cgtctcgagc tgccggcgcc agcgccgttg
     2341 tcgccgaagg agttgctgca ggcagccgga tttggcgata ccgagggact ggtgctgatc
     2401 gtcgacgaca ctgtgattcc gcgcgccgac tggcagcggc cgttgatcga cgacggcacc
     2461 gcggtcaccg tgctatccgc cttcgagggt ggctgacggc gacgccgagc ccgggccggt
     2521 gcttagcgca agcgatgtga tgcgccgggc gagggtgccg ctttcccggt tgtcggtgtc
     2581 cagccgctgc tagaagggat gacccatgcg caggtagaaa acatagtccg agctctcgcc
     2641 cttggcgatt tcggaacgga tgacgatacc ggcctcgggt tttaacgtat agatgacgcc
     2701 ggcgccggcc gaggcgaaca gattttccga ttgttcgcaa tcggacaagc catcgtagag
     2761 gcaggcgatg ccggcgaaca ccgccattcc ccacctggaa cccaggctga tgcggtcctc
     2821 cgcctgcaca tgggtatagt taggagccag gtaattgccg cgcacgtagc cccgcaggct
     2881 gatcgacgca taaccgccga tcggggcgtc atcggtccat ttgccgctca gctgccaggc
     2941 gagtagatgc gattcgacga tctcgcggta ccggctatag tcgatccggt aagtgtcgaa
     3001 gctgacgtcg ccgccgaggc ttttgctgta ggcgatattg tgaatcacca ggtaatcgcc
     3061 gtcaccgggg ttgcgaatct tgtcgcggct gtcgtattcg gccaccaggc ccgcgccgac
     3121 ggaatcgaat ccggtgagac cgattcgatc cagaatctcg ccgagcagat cgtcggcgcc
     3181 gatcacgtag ttgctggaga cggcctgcgc gccgaggtac cagttatcgg tgatgccgcg
     3241 gtaatagcgc aagaattcgg cgttcagctg gtcggttgtc tgcgccggga ttccgctgcc
     3301 gaggaagtcg tcgtagtcgt tgttgatctt gccgcgggcg acaccgacca gcagtttttg
     3361 ccggttggcg tcgaaataca gttggccgaa tacgccgccg acataggaat cgctgtcgct
     3421 gtaactaatg ctggtcaggg ccatcgactg gcgcgattcg ggatcgattc ggaacaggta
     3481 gccacccatc gcaccaaccg aggtaccgag cttcgggtcg ctactcatcg tcggcaccag
     3541 cagccacggc gattcgtccg ccggctcgtc gcctgctgcg cgggcggcgc acgacagcat
     3601 caaaatcagc caggcgcaac ggcggatcag gcgcattcga cggcggttat cgagatgggc
     3661 aagcttgccg acggaagcca ccgggaattg cggcccggga tcttggcgag gttcgcgagt
     3721 ctagacctgg cgttgcaaac ccgggttgcg ggcatcaccc ggaagtcgcc gcgaggacgt
     3781 agagcatctc gaggcccagc gtggcgccgg ccagcgacgt aatctgcgca tggtcgtagg
     3841 ccggcgcgac ctcgaccaga tccatgccga ccaggttgat gccggtaagt ccacgaatga
     3901 ttttcaatgc ccggtccgag gtcaggccgc cggcgaccgg ggtgccggtg ccgggcgcat
     3961 aggccgggtc gaggcaatcg atgtcgaagg tcaggtagca gggtgagtcg ccaacgcggg
     4021 ccctgatgcg cgcgacaatc tcgtcgacgc tgatttcgtt ggcctgcgcc gcgtcaatga
     4081 cttcgaattc gtgatcgctc gccgtataca tggtgcgaat gccgacctgt atgctgcgct
     4141 cgggatcgat gatgctctcg cgcggcgcgt ggtaaaacat gctgccgtgg aaataaaccg
     4201 agtccggatg gtcgtgggta tcggtgtggg cgtcgaaatg aatcattgcc agcttgccgt
     4261 gctgtttggc atgcgcgcgc agcagcggca gcgtgatgaa atgatcgccg ccgaaagtca
     4321 gcgtcgtctt gccggcggcg agcagtttgc catagtgttc ggcgaccgat gcgaacagcg
     4381 cctcgtggtt gccgaatgga tactcgaggt cgccgtagtc ggccacccgc agtctttcga
     4441 acaggtcgaa ggtccagggc cagcgatttt cttcccaacg cagctgggcg cttgcggtgc
     4501 gaatcccgag tgggccctcg cgcgtgcccg agcgaccgct ggtaccgagg tcgtagggca
     4561 gaccgacgac gaatacgtcg gccgcgtcca ggtcgcgcgt cagcggcagg cccaggtagc
     4621 catggatatt ggaaaacatc gagtagctgc tggggaaatc tttatcggtc attacaggta
     4681 ccagtcgtat tcgcgggcgc tgatgtaatt gtcgaaggcg gcggtttccg cggcgcggtg
     4741 gcggcagtag aggtcgacga actccgcgcc gaggctgtta tcgagcacct ggctcgcgcg
     4801 cgtcgcggcg agcgcgtcgg cgagattcag tggcaggctt tcgtcgcggc cggtgaccgc
     4861 ctcttcggtc gccgggcccg ggtcgagcgc ggcgtcgata ccgtgcagca tcgccgccag
     4921 cgtcaccgcc atcgccaggt agggattggc gtcggctccg ggcacccggt tctcgacccg
     4981 ccaggcgccc tgctgtgcga gcggcacgcg gaacgcgacg gtacggtttt cgtcacccca
     5041 ggtcaggctc accggcgcgc agctgatgcc gccgcggtag cgccggtagg aattgacgtt
     5101 cggcgcccag aaggacatcg ctgcgggcat ggtttccagc aggccgccga ttgcgtgccg
     5161 cagtcgcggg tgcgctcgct cgccggcggc gaaaatatta tcgccgttag cgtcgaccac
     5221 gctgatatgc atatgcaagc cgttgccggc ctgctcgagc agcggcttgg cgatgaagct
     5281 ggcgcagaat ccgtgttttt tggccacctc gatcacggta cgcttgaaca ggcaggtttc
     5341 gtcggcggcc cgcagcgtat cgtcgtggtg gttgaagttg atctcgaatt gaccgggccc
     5401 gagttccgac gaaatcgcgc cggtatcgat tcgctgcagc tcgcaggcgc ggacgatatc
     5461 gtcgatcacc gcctcgaagt cgccgacccg cgtgctcgac agtacggtgg cggtatcctc
     5521 gcggccggtt ttcgggttac gcgcgacgcg cagcccgcca tcttcgccgc gccggccgtc
     5581 gaacaggtag aactcgagtt caaaggcgac cgtcggtcgc cagttgcgct cggcatagct
     5641 gtcgacgacc cgttgcagca cgtaacgggg ttcgaaccag gccggcgcgt cgctgtgaca
     5701 ggtggtcgcc agtatctgcg cgcgcgggct gttgccccag tcgttgacgc acaggctgct
     5761 gtcgaccagg ctgaagtggg cgtcggggtc gccgtcgtcg atgccgtaat acatgccttc
     5821 gagcggttcg ccgagcgtgt tcagcacgaa catggatgcc ggcatcgcca ggccgtcgcg
     5881 cacgaacgac tcgagcttgt cgagcgggta gcgcttgccg aagaaatgac cgcacaggtc
     5941 ggtcgacagg acttcgagct gcgcgagccc ggggtgagcc gccgtcaggc tgtcgatctt
     6001 caggttcaac tgtttaccac cgctactatt catctctcga ccaggagtta cccgttaggc
     6061 agttgtttgt gatcacgtaa attcgaaatt gccacgccgg acagaatcgc gctaaagatt
     6121 acccgttaat gatcgtgatc actaattgtt ttaaacggga gtttcaggcg acgccgcgga
     6181 aataatcgtg aatcccttcg atcgtattga tcgacgcgat tccatcgcga atatcggctt
     6241 cgatcgcgcg tttcaggccg aaaccgtcgc gttttttgag cgcgtcgatg gcttcgtgat
     6301 gacggtcgac cagataaacc ttttccagct tgtcgatggc gatgcgcgag aagggtccga
     6361 gttgcagcca caggctttcg atcagcggca ccgaaatctg gaacgggttg gtgctgtaga
     6421 ggtgacggtg aaaggcctgg ttttcgaggc tgatgtggtc ggcgtcgttg gcttcgatgg
     6481 cggcatcgat tgccgcgtcg atttgttcca gtgcttcgat atcggcgtcc ttgacatagg
     6541 gcagggcgcg ctcggcggca tgggtctcga gcaggatgcg caggtcgcac aattcgccga
     6601 atttctccgc ggtcatcagc ggcacgatga cccggcgatt gttcttgacc tcgaccgccc
     6661 cctcgcaggc cagccggtgc agggcttcgc gaaccggcat cgggctgacg tgcaggagtt
     6721 cggcgaggcc gcgaatggtc agcggcttgc cgggttcgat gcgcccgcac agcaggttgc
     6781 tgcgcagtaa ctggtaaacc cactggtggg ttttcatctg ttccgatttt ttcagcatca
     6841 gcagctttaa agccgccgaa tccttggatt tcatgcgccg gtgtccaggc ttcgagggcc
     6901 agagatttaa tcacaaaaag cgcatcacga ccaatcgtgc ggttgccagc atcggcaact
     6961 ggcgcataag atatgatcac attcgcgagt tcacggcgag taaatctacc ggccgctggg
     7021 cgcgaatgct atatttcgtc gtcctttaac ccgaggtggt caagcaatga aaatcggtat
     7081 tctcgaagcc ggcctgctgc gcgaggaaat ggcttcgcgg ttcgatcctt acccccgcat
     7141 gttcacggat tttctcgcgc tcgcgcatgc tgatttcgac taccgcacgg tcagcgtggt
     7201 gcgtggcgag atgcccgccg gcgtgtacga ttgcgacggc tggctgatta ccggctcgcg
     7261 ccacggggtc tatgatcgtc tcgactggat gccgccgctg gaggaattca ttcgccaact
     7321 cgcgaccgcg cgagtgccgt tgatcggggt ctgtttcgga caccagatca tcgcttccgc
     7381 gctcggcggc gaagtcgtca agtcggagca gggctggggc gtcggtttgc atcgctaccg
     7441 ggtcgaccgc cggcacgcat ggatgcacgg cgacgaggca gagacgtcgc tgtacgcctt
     7501 ccaccaggac caggtgatca ccggccccgc cgaagccgaa gttttcctga gttcggaatt
     7561 ttgtccctac gccggcctca gttacggcga ttccattatc agcgtgcaag cgcatcccga
     7621 attcggcgcc gaatacgaaa ccgcgttgct ggaagcctac ggcggcagcg tggtgccggc
     7681 agcagtcgcc gccgccggcc tcgattcgat gcgtgacggc gcgtgcgccg ataccgcgct
     7741 gctggcaaac tggtttgcga actttttctt gtcccggcga ctgcaagcgg ccggcaatgg
     7801 ctgagactac cgccaac
//
LOCUS       DATCZV010000313         5232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_342874, whole genome shotgun sequence.
ACCESSION   DATCZV010000313 DATCZV010000000
VERSION     DATCZV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342874"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1085..2887)
                     /gene="rpoD"
                     /locus_tag="VIW27_07040"
     CDS             complement(1085..2887)
                     /gene="rpoD"
                     /locus_tag="VIW27_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5739465.1"
                     /translation="MMDQDPQSRLKELIAKGKEQGFLTYTEVNDHLPDGIVEPEQIED
                     IIRMINDMGIKVHESAPTDSDDILSDTETEPDEDAAEEAAAALASLDSEFGRTTDPVR
                     MYMREMGTVELLTREGEIKIARRIENGLNQAMSSLARYPDTMRLIIERYNEVREEEVK
                     LSDLLVGFVDPEEDPNSIPLPASAQSSDDDDNADVDDSGPDPAEAEKRFTSIERAFNR
                     AMNCVAKNDEKGYERNMNRAVKELMEIKLLPLFIDRLALNLKDVINRIRSNERTILDL
                     CVNQAQMPRKTFIETFKANETNLKWIDGHLKSGEKYVRALEGVKDEILRYQKRLKKIE
                     KDTHLSIHDIKEINRKMSIGEAKARRAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQ
                     EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
                     RISRQMLQELGREPQPEELAERMDLPEDKVRKVLKISKEPISMATPIGDDEDSNLGDF
                     IEDTNVLLPADTATNAGLSESTREILSSLTPREAKVLRMRFGIDMNTDHTLEEVGKQF
                     DVTRERIRQIEAKALRKLRHPSRSEQLRSFIDAD"
     gene            complement(2968..4689)
                     /gene="dnaG"
                     /locus_tag="VIW27_07045"
     CDS             complement(2968..4689)
                     /gene="dnaG"
                     /locus_tag="VIW27_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5739466.1"
                     /translation="MSGRIPRSFIDNLLNRVDIVEVIDSRVPLKKKGREYWACCPFHG
                     EKTPSFSVSPAKQFFHCFGCQKSGNAIGFLMDYDHMEFVEAIETLAGGQGMEVPYEQG
                     SAPPAAARAGLDPMYAAMEQCAAYYQAELKNSATAIDYLKNRGISGQTAKTFGIGYAP
                     PGWNNLAGDEKLLTETGMLVQKDAGNRYDRFRNRLMFPIRDRRGRVIAFGGRVVDPED
                     NPKYLNSPESPLFHKGETIYGLYELRKAVTNIERIYITEGYMDVVALAEHGVETAVAT
                     LGTAINNRQVEALFRVCRQLVFCFDGDAAGKKAAWRALEACLPSLKEGRLARFLFLPE
                     GQDPDSYIGEYGKDAFEALVEQASTLTHYLFEMLLAECNIRTLEGRAEFLDRLRPYFG
                     QIPLQSLKDQILVEVEQRLGQKIDGRMLRLLGAERPRREPPTRLPEQHWTPTRLAINL
                     LFKRPSLALACGSQDELADSGIPGVELLLKILDRIHEDPQISTQNLLDRFQGDEYENH
                     LYKLAAMDPPAEDDSLERMFADCLQQLRKQYIGERRKRLIGKLQTGEALSEAEKLEHR
                     KLFSSQN"
     gene            complement(5013..>5232)
                     /gene="gltD"
                     /locus_tag="VIW27_07050"
     CDS             complement(5013..>5232)
                     /gene="gltD"
                     /locus_tag="VIW27_07050"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEY5739467.1"
                     /translation="VHEGMLNQLRVELDGRGNVRANEADYRTSVDKIFACGDARRGQS
                     LVVWAIREGRQAARAVDHFLMGRTELPR"
BASE COUNT          840 a         1524 c         1683 g         1185 t
ORIGIN      
        1 gttggcgggt tttaccttgg catgggtaga aagctgctaa aacaacgata cccggcctga
       61 tccggacaag gccgatgggt gggggccaag caaaatattt cacaagctct gaatagaatg
      121 ggtggattga agtggctgac ccgtcatccg cgacgttagg acttgctgac gattattttt
      181 ttccgatccg gtaaaaatag taactagagg taaattgttg gtgggcccgg ctggactcga
      241 accagcgacc aatggattca cattctctac tgctttcgca atagtgcgga ctatctcatc
      301 acccgcgtgg ggtgtcggac gctgatggcg tattacgcga cagggtcgtg ccgcaccgcc
      361 tagtctctga accttcctga aacgcctttc aggcttggct gctgatcggc atatcccgaa
      421 gggacttagc tttccagcaa ttcatccgat ttttccgtac ggcattgctg ccgaaggtca
      481 cacttttgta tgagtccact gctctaacca actgagctac aggccccctg gcttgtcttt
      541 ttcacgccat gcggcgttgg attgcgcgag cgcttggtca cataccggca tgtatgctcc
      601 cggcgcgctc ggaaatccgc cttgcctggc gcgaaaaatc ctgcgccaga tttccgacgc
      661 aggagaaaca tcattatagc aacttgcgct tggtcgcgta gcttgagcta cgctcccggc
      721 gacggtcgcc aaatccgcct tgcctgacgc caaaaatcct gcgtcagggt gacgatccca
      781 acaaccaggg attgccattc aggagaagcg ggaatcttat acggactggc ttattttgca
      841 aggctcgggc tgcggtttgc actgtctgtt ggttgggatg gattccggat aacgcttgcg
      901 gggtttccgg aatgactttg gagtggggcg gttgagaaaa aagccgtccg gctgggcggt
      961 ctttgaattt tgtttggaga tccccgcttt cgcggggatg actcctggac tgcggggcgg
     1021 ctcctggact gcggggcggc tcctggaccg tgggatgacg cccggggcgg tgtcgacggc
     1081 ggggctagtc ggcgtcgatg aagctgcgca gttgctcgga gcggctcggg tggcgcagct
     1141 tgcgcagggc cttggcttcg atctggcgaa tgcgctctcg ggtgacgtcg aactgcttgc
     1201 cgacttcctc gagggtgtgg tcggtgttca tgtcgatgcc gaagcgcatg cgcagcacct
     1261 tggcctcgcg cggcgtcagg ctcgacagga tctcgcgggt cgattccgac aggccggcgt
     1321 tggtcgcggt gtcggccggc agcagcacgt tggtgtcctc gatgaaatcg ccgaggttag
     1381 aatcctcgtc gtcgccgatc ggcgtggcca tcgagatcgg ctccttggag atcttcagca
     1441 ctttgcgcac cttgtcctcg ggcaggtcca tgcgctcggc gagctcctcg ggctgcggtt
     1501 cgcggccgag ctcctgcagc atctgccttg agatgcggtt cagcttgttg atggtttcga
     1561 tcatgtgcac cggaatacga atggtgcgcg cctggtcggc gatcgaacgc gtgatcgcct
     1621 ggcgaatcca ccaggtggcg taggtcgaga acttgtagcc gcggcggtat tcgaacttgt
     1681 cgaccgcctt catcaggccg atgttgcctt cctgaatcag gtcgaggaac tgcaggccgc
     1741 ggttggtgta cttcttggcg atggaaatca ccaggcgcag gttggcctcg atcatttcct
     1801 tcttcgcgcg ccgcgccttg gcttcgccga tcgacatctt gcggttgatt tccttgatgt
     1861 cgtggatcga caaatgggtg tccttctcga tctttttcag tcgcttctgg tagcgcagga
     1921 tctcgtcctt gacgccttcc agcgccctga cgtatttctc gcccgatttc aggtgaccgt
     1981 cgatccactt caggttggtc tcgttggcct tgaaggtctc gatgaaggtc ttgcgcggca
     2041 tctgcgcctg gttcacgcac aggtcgagga tggtgcgctc gttgctgcgg atgcggttga
     2101 tcacgtcctt caggttcagc gccagccggt cgatgaacag cggcagcagc ttgatttcca
     2161 tcagctcctt gaccgcgcgg ttcatgttgc gctcgtagcc tttctcgtcg ttcttggcga
     2221 cgcagttcat cgcacggttg aaagcgcgct cgatcgacgt aaagcgtttc tcggcttccg
     2281 cgggatcggg gcccgaatcg tcgacgtccg cattgtcgtc gtcgtcgctg gattgcgccg
     2341 aggccggcag cgggatcgag ttggggtctt cctcggggtc gacgaaaccg accagcaggt
     2401 cgctgagctt gacttcttcc tcgcgcacct cgttgtaacg ctcgatgatc agccgcatgg
     2461 tgtccggata gcgcgccagc gaactcatcg cctggttgag gccgttctcg atgcgccgcg
     2521 cgatcttgat ctcgccctcg cgggtgagca gctcgacggt gcccatttcg cgcatgtaca
     2581 tgcgcaccgg gtcggtggtg cggccgaatt cactatccag tgacgccagt gcggcggcgg
     2641 cttcctcggc cgcgtcctcg tcgggctcgg tctcggtgtc gctcaaaatg tcgtcggagt
     2701 cggttggcgc cgattcgtgg accttgatcc ccatgtcgtt gatcatgcgg atgatgtcct
     2761 cgatttgctc gggctcgacg atgccgtcgg gcaggtgatc gttgacctcg gtataggtca
     2821 ggaatccctg ttccttgccc ttggcaataa gttcttttaa ccgggattgc gggtcttggt
     2881 ccatgatgcc tcgatttggt tcagtggtgc gcgggtaaca ccccagtata taagactttt
     2941 tttgcttttt ccaagagctg ccgtgcttta attctggcta ctgaacaatt tcctgtgttc
     3001 gagtttttcg gcctcggaaa gtgcctctcc ggtctgcaat ttgccgatca ggcgcttgcg
     3061 gcgttcgcca atatactgtt ttcgcagctg ttgtaaacag tccgcgaaca tacgctcgag
     3121 actgtcgtcc tcagccggcg gatccattgc cgcgagcttg tagagatggt tttcgtactc
     3181 gtcgccctgg aagcggtcga gcaggttctg ggtgctgatc tgcggatcct cgtgaatgcg
     3241 gtcgaggatc ttcagcagca gctcgacgcc cggaatgccg ctgtcggcga gctcgtcctg
     3301 gctgccgcag gcgagcgcca ggctgggccg cttgaacagc aggttgatcg ccaggcgggt
     3361 cggcgtccag tgctgctccg gcaggcgggt cggcggctcg cgcctgggcc gctcggcgcc
     3421 cagcaggcgc agcatacggc cgtcgatctt ctgccccagg cgttgctcga cctcgaccag
     3481 gatctggtcc ttgaggcttt gcagcggaat ctgcccgaag tagggccgca ggcggtcgag
     3541 aaactcggcg cgcccctcga gggtgcgaat attgcactcg gcgagcagca tctcgaacag
     3601 gtaatgggtc agcgtcgagg cctgctcgac cagcgcctcg aaagcgtcct tgccgtattc
     3661 gccgatgtaa ctgtccgggt cctggccctc gggcaggaac aaaaagcgcg ccagccggcc
     3721 ctccttgagc gacggcaggc aggcctcgag cgcgcgccag gcggccttct tgccggctgc
     3781 atcgccgtcg aagcagaaca ccagctggcg gcagacgcga aacagcgctt cgacctggcg
     3841 gttgttgatc gcggtgccga gggtggccac cgcggtctcc acgccgtgct cggcgagcgc
     3901 gaccacgtcc atatagccct cggtgatgta gatgcgctcg atgttggtga ccgccttgcg
     3961 cagctcgtag aggccgtaga tcgtctcacc cttgtggaac agcggcgatt ccggtgaatt
     4021 caggtacttg gggttgtcct cggggtcgac gacgcgaccg ccgaaggcga ttacgcggcc
     4081 gcggcggtcg cgaatcggga acatcagccg gttgcgaaag cggtcgtagc ggttgccggc
     4141 gtctttttgt accagcatgc cggtctcggt aagcagtttt tcgtcgcccg cgaggttgtt
     4201 ccagcccggc ggcgcgtagc cgatgccgaa ggttttcgcc gtctggccgc tgatgccgcg
     4261 gtttttcagg tagtcgatcg cggtggcgga gtttttcagc tcggcctggt agtaggccgc
     4321 gcactgctcc atcgccgcat acatcggatc gaggccggcg cgtgcggcgg cgggcggtgc
     4381 gctgccctgc tcgtagggca cttccatgcc ctggccgccc gccagcgtct cgatcgcctc
     4441 gacgaattcc atgtggtcgt aatccatcaa aaacccgatc gcgttgccgg atttctggca
     4501 gccgaaacag tgaaagaatt gcttggccgg gctgaccgag aacgacggtg ttttttcgcc
     4561 gtggaacggg cagcaggccc agtactcgcg acccttcttc ttcagcggca cgcggctgtc
     4621 gatgacctcg acgatgtcga cgcggttcag caggttgtcg atgaaactgc gtggaatgcg
     4681 tccgctcatt gaatcgcgtc tactccgggg cgtttgcagg gccggtcatt ttcgcacgat
     4741 tggtcggtga gcagtagacc gttcatcggc gatcgcgatg tcgtcatgcc gggctcgacc
     4801 gatacgtcgg accgccggca tccatcgaaa atcggctcga tagtgcccag gcccgccatg
     4861 ctttgcgagg ataatatcgg atcttgggga tagattgcgg ctcgggggcc gcaatgacgc
     4921 ggattgcgga aacgcggcgc gggcgggccg cgggcggcgc cgcaatgacc gggtttccga
     4981 aacgcggctc gaaggtcgca gcgacgcggc ggttagcggg gcagctcggt acggcccatc
     5041 aggaagtggt ccacggcgcg cgccgcctgg cggccttcgc ggatcgccca gaccaccagc
     5101 gattgtccgc ggcgtgcgtc gccgcaggcg aatatcttgt cgaccgaggt gcggtaatcg
     5161 gcctcgttgg ccctgacgtt gccccgcccg tcgagctcga cccggagctg gttgagcatg
     5221 ccctcgtgca cc
//
LOCUS       DATCZV010000314         4319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_237131, whole genome shotgun sequence.
ACCESSION   DATCZV010000314 DATCZV010000000
VERSION     DATCZV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237131"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..419
                     /locus_tag="VIW27_07055"
     CDS             204..419
                     /locus_tag="VIW27_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739468.1"
                     /translation="MNDDDLRPLHGSRTPCRVFAPLLPGAPSRRITGPVIGLQPDSPA
                     AAALAPFTRRPATLVAGRSQPLAGGHH"
     gene            438..1238
                     /gene="bioC"
                     /locus_tag="VIW27_07060"
     CDS             438..1238
                     /gene="bioC"
                     /locus_tag="VIW27_07060"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEY5739469.1"
                     /translation="MDLLNPPRLDKRFIKKSFNRAAKSYDDAAILQEEVLHRLLQRLP
                     YIRHQPQIVVDLGCGTGKGIAGLARHYPRARIIGMDIAEQMLARARPRFGRLRRKRLV
                     AADLEQLPFAAQSFDLVFSSLALQWSNAAGRECARVSRPGALLMFASFGPGTLGELAA
                     AWQMLDSRPHVHRFVDMHDVGDAMLAAGFEQPVVDAEVIRLEYGELRGLLDDLSNAGA
                     SNADIDRCRGLTTPRKLARLEDCYRQHGMRDGKFLASYEVVYGHAWCR"
     gene            complement(1249..1470)
                     /locus_tag="VIW27_07065"
     CDS             complement(1249..1470)
                     /locus_tag="VIW27_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY5739470.1"
                     /translation="MSIAKIIIVVLLLLIVLSLAAGMFSLVRERGESSRTATFLTLRI
                     ALSIITFVFIAVAFYMGWIQPHGLLPPGA"
     gene            1528..2244
                     /locus_tag="VIW27_07070"
     CDS             1528..2244
                     /locus_tag="VIW27_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY5739471.1"
                     /translation="MIIGKRVFVPTAWSIVLTAAGVALFVSLGLWQLERADYKTGLER
                     KFERRLAQDYRPLDSDEDFSDIQYRKLRLKGRYDLDHQFLLDNQVHEGRAGYHVLTPF
                     RLADSDFLLLVDRGWAAWGERRSPLPPIRPPLQAGTTSGIAFVPGEPALKLGEPMPGG
                     DWPRLITYIDIDELRQYFADDLLPMVLWMSPETPGVYLRDWDPVWLPPEKSRAYALQW
                     FSFAAISLLLFVLLNLRKIE"
     gene            2241..2870
                     /locus_tag="VIW27_07075"
     CDS             2241..2870
                     /locus_tag="VIW27_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739472.1"
                     /translation="MNPETSTGPGKAAPENLFWHRVKLLALVAVFLAPFVGGWLAFYV
                     FELRPDSGNYGMLVKPVRKIDWPPLVSVDGERSENGFGGKWRFVLFAGDACDEPCRAN
                     LYYLRQIRILLGRDTARLQNLMIVGRPLDDSLREFLRDFPHLKVIEAYRRPQFDAQFA
                     IDGEAAVGDSAKIYLVDPDDNLMMHYPAKNDQNRVLEDIKRLMKISQIG"
     gene            2960..3862
                     /gene="cyoE"
                     /locus_tag="VIW27_07080"
     CDS             2960..3862
                     /gene="cyoE"
                     /locus_tag="VIW27_07080"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5739473.1"
                     /translation="MAALTLNSWTHYYNLTKPKVVLLIVFTAVIGMLLAADGAIPLDI
                     FVFGLLGIGLAAASGAAINHVVDEHIDRIMERTRKRPLVSGALDQKSALVFALSLGAL
                     GIGLLVAFVNLLTAVLTFFSLVGYALIYTMYLKRATPQNIVLGGAAGAAPPLLGWTAV
                     TGTVDTEALLLFLIIFVWTPPHFWALAIRRREEYARAEIPMLPVTHGVLFTKIQILLY
                     TILLVVVSMMPFIVQMSGLIYLAGALALGVGFLYYAIKLYRDRQPNAIAMKTFGYSIF
                     YLSLLFMFLLADHYARVFIRGWFL"
     gene            complement(3872..4102)
                     /locus_tag="VIW27_07085"
     CDS             complement(3872..4102)
                     /locus_tag="VIW27_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEY5739474.1"
                     /translation="MKQSTQENAEKALKLGFWQLLKSTLSAFIGVQSNANRERDFSHG
                     KFSHFIWMGLLFGVVFVLTLVGVVQLVLQLAS"
BASE COUNT          758 a         1311 c         1377 g          873 t
ORIGIN      
        1 ggttgttcga accaccgctg cgcggatcgt cgaaacccat gatgcggctc aagatgatcg
       61 ctgccgacag cgagccgagc aggtaggcga agcagagcag ggcgagggtt tcgagcatgc
      121 gagtcggcgg tcgatgaaaa acccgggaac tctaccagat ttggcctgtg ctggcagcag
      181 cgggaatccg ttacaatgcc gcaatgaatg acgacgatct gcgcccgttg cacggttcac
      241 gcacgccctg ccgcgtgttc gcgccgctgt tgccgggcgc tcccagtcgt cgaatcaccg
      301 gaccggtgat cgggttgcag ccggattccc cggccgctgc cgcgctcgcg ccgtttacgc
      361 ggcggccggc gacgctcgtc gccggccgtt ctcagccgct cgccggcggc caccactgaa
      421 tcgttcccgg agtcattttg gatctgctca acccgccacg actggacaag aggtttatca
      481 agaaatcctt taatcgtgca gcgaaaagtt acgatgatgc agctatcttg caagaagaag
      541 tgttgcatcg gttgctgcag cggctgccgt acattcgtca ccagccacag attgttgtcg
      601 atctcggctg cggcacgggc aagggtatcg cgggccttgc gcgccactac ccgcgcgcgc
      661 gtatcatcgg catggatatc gccgaacaga tgctggcgag ggcgcggccg cgcttcggca
      721 ggctgcgccg caaacggctg gtggcggccg atctcgagca attaccgttc gcggcgcaaa
      781 gcttcgacct ggtgttttcg tcgctggcgc tgcaatggag caacgcggcg ggccgggagt
      841 gcgcgcgcgt ctcccggccc ggcgcgttgc tgatgttcgc cagtttcggg cctggcacgc
      901 tcggcgagct ggcggccgcc tggcagatgc tcgattcacg gccgcacgtg caccgtttcg
      961 tcgatatgca cgacgtcggc gatgccatgc tcgccgcggg tttcgagcag ccggtggtcg
     1021 acgccgaggt cattcggctg gagtatggcg aactgcgcgg cctgctggac gatctcagca
     1081 atgccggtgc cagcaacgcc gacatcgacc gctgccgcgg attgacgacg ccgcgtaaac
     1141 tggcgcgact cgaggactgc tatcgccagc atggcatgcg tgacggcaag ttcctcgcca
     1201 gctacgaagt ggtctacggg cacgcatggt gccgctgaag ccgcgggatc aggcgccggg
     1261 cggcagcagt ccgtgcggtt ggatccagcc catgtaaaat gccaccgcga tgaacacgaa
     1321 ggtgataatg gacagcgcga tacgcagtgt caggaaggtg gcggtgcggc tcgattcgcc
     1381 gcgctcgcgc accaggctga acatgccggc ggcgaggctc agcacgatca ataacagcag
     1441 gacgacgatg atgattttgg cgattgacat gggtggcgct gtggatacgc ggggcaccag
     1501 tttaccgcat catcgggcga tgtcgagatg attatcggca agcgcgtgtt cgtgccgacc
     1561 gcctggtcga tcgtgctgac cgcggcaggc gtggcgctat tcgtgtctct cggcctctgg
     1621 caactcgagc gcgccgacta caagaccggg ctcgagcgca agttcgagcg tcgcctggcg
     1681 caggattacc ggccgctgga ttcggacgag gatttcagcg acatccagta ccgcaagctg
     1741 agattgaagg ggcgctatga tctcgaccac cagttcctgc tcgacaacca ggtgcacgag
     1801 gggcgcgcgg gttaccatgt gctgacgccg tttcggctgg ccgacagtga tttcctgctg
     1861 ctggtcgacc gcggctgggc cgcgtgggga gagcggcgtt cgccgctgcc gccgattcgg
     1921 ccgccgctgc aggccggtac caccagcggc atcgccttcg tgccgggcga accggcgttg
     1981 aagctcggcg agccgatgcc cggcggcgac tggccacggt tgatcaccta tatcgatatc
     2041 gacgaattgc ggcaatattt tgcggacgat ttactgccca tggtgctgtg gatgtccccg
     2101 gaaactccgg gcgtctacct gcgagactgg gatccggtct ggctgccgcc ggaaaaaagc
     2161 cgcgcctacg cgctgcaatg gttttcgttt gccgccatat cgctgctatt gttcgtgcta
     2221 ctcaacctgc ggaagatcga atgaatcccg aaacgtcgac cggacccggc aaggccgcgc
     2281 ccgaaaacct gttctggcat cgcgtcaagc tgctcgcgct ggtggcggtg ttcctcgcgc
     2341 cgttcgtcgg cggctggctg gctttttacg tcttcgagct gcgtccggat agcggcaatt
     2401 acggcatgct ggtgaaaccg gtccgcaaga tcgactggcc gccgctggtc agtgtcgacg
     2461 gcgagcgcag cgagaacggc tttggcggca agtggcgttt cgtgctgttt gccggcgacg
     2521 cctgcgacga gccttgccgc gcgaacctgt actacctgcg gcaaatccgc attttgctgg
     2581 gccgcgatac cgcgcgtctg caaaacctga tgatcgtcgg ccgcccgctc gacgacagcc
     2641 tgcgcgaatt cctgcgcgac tttccgcacc tgaaggtgat cgaggcgtat cgccggccac
     2701 agttcgacgc gcagttcgcg atcgacggtg aggcggcggt cggcgacagt gcgaagattt
     2761 acctggtcga tccggacgac aatctgatga tgcattaccc ggcgaaaaac gatcagaatc
     2821 gggttctcga ggatatcaag cggctgatga aaatatcgca gatcggctga gtctgatatc
     2881 attcggcgat tgaagcccgg cgccaaaacc tgtataacgg cgcgctttgc aacgcgagac
     2941 cgatcctgac gatcagccga tggccgcact gacgctcaat tcctggacgc attattacaa
     3001 tctgaccaag cccaaggtcg tgctgctgat cgtgtttacg gcggtgatcg gcatgctgct
     3061 ggctgccgac ggtgcgattc cgctggatat tttcgtcttc ggcctgctgg gcatagggct
     3121 ggccgcggct tccggcgcgg cgatcaatca cgtggtcgac gagcacatcg accgcatcat
     3181 ggagcgcacc cgcaagcgcc cgctggtcag cggcgctctc gatcagaaat cggcgctggt
     3241 gttcgcgctg tcgctgggcg cgctcggcat cggcctgctg gtggcgttcg tcaacctgct
     3301 gaccgcggtg ctgacctttt tttcgctggt cggatacgcg ctgatctata ccatgtacct
     3361 gaagcgcgcg acgccgcaga atatcgtgct cggcggcgcg gccggggcgg cgccgccgct
     3421 gctcggctgg accgcggtta ccggcaccgt cgataccgag gcgctgttgc tgttcctgat
     3481 catattcgtc tggactccgc cacacttctg ggcgctggcg attcgccgcc gcgaggagta
     3541 cgcgcgcgcg gagattccga tgctgccggt cacccatggc gtgctgttca ccaagatcca
     3601 gatcctgctg tacacgatcc tgctggtggt ggtctcgatg atgccgttca tcgtgcagat
     3661 gagcggcctg atctacctcg ccggtgcgct ggcgctcggc gtcggcttcc tctactacgc
     3721 gatcaagctg taccgcgacc ggcagccgaa cgcgatcgcg atgaaaacct tcggctactc
     3781 gattttttac ctgagcctgc tgttcatgtt cctgctcgcg gaccactacg cgcgggtgtt
     3841 catccgcggc tggttcctgt aagcgccgct gtcagctcgc cagctgcagt accagttgca
     3901 ccacgccgac cagcgtcagc acgaatacga cgccgaaaag caggcccatc cagatgaaat
     3961 gggaaaattt cccgtgactg aaatcgcgtt cgcgattggc gttgctctgc acgccgataa
     4021 aggccgaaag cgtgcttttc aacagctgcc agaaaccgag cttgagcgct ttttcagcgt
     4081 tttcctgcgt gctttgcttc acaagtgaga ttccgtatta ttcgacgatc gtgacctacg
     4141 ggccagcggc ccgtcgtaag tgccggacaa ttgtaaagca tcgtgatcag tatcgataag
     4201 cacaattcga caaattcgct acgcctggtg ctgatgccga actgttcgat cagctggcgt
     4261 ggcctgctga ttttttacct tgccaactgc gtcgtgctgc tggcgatcgg ggtgttttt
//
LOCUS       DATCZV010000315         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_501678, whole genome shotgun sequence.
ACCESSION   DATCZV010000315 DATCZV010000000
VERSION     DATCZV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501678"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1299
                     /locus_tag="VIW27_07090"
     CDS             55..1299
                     /locus_tag="VIW27_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY5739475.1"
                     /translation="MSSARDTVKWLLGAVLLGLLGWLLYINIEFYDETEQASWSSKAL
                     SNPYLAAQQFMERSGIDVRDVQGLGRQQELNNVGTLFFSDPGQIQTPRQLQQVLEWLQ
                     GGGNVLYAATAVGDEDDLLLAEVGVEAAWRDYDDDGDQQEKSVAETMREYNRQLAEGK
                     TREQIAEQQEEEGISLTRVVFGDEIGDLEVAFDNEIVLAHAYVDDDDTSGAHQPFSWS
                     SSDYGVHMMQFDIGSGLLTIVSDPQVWTSYSIDRHDHAYLLWLLSSDGGDFAMLRSVV
                     RDTIWELMMRNASELLIAGTLLLLLWVWFRGARFGRLLPRDRSRRRALGEHFTSVAHY
                     LWHRRDGAYLIAALRQQVLRRASLTLAGFAGADSRRQHELIAERCDLEANAIGRALGE
                     NNFNEATFVSTVRLLKRIEQAL"
     gene            1296..2246
                     /locus_tag="VIW27_07095"
     CDS             1296..2246
                     /locus_tag="VIW27_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5739476.1"
                     /translation="MNESSNAIPAALAALREHVNSRLIGQARVVDEVLIALLAGGHVL
                     IEGVPGLGKTLLVQTLAAGIGGQFKRIQFTPDLMPGDVTGHVMFDMAQSKFVLKHGPV
                     FTNLLLADEINRAPAKTQAALLEVMQERQVTLDGQSMPVGRPFMVLATQNPIEQEGTY
                     ALPEAELDRFIIKINIDYPEAHDEVQIATRYALENFQQQASEMRSTLDVEAAQRQCAA
                     IAVDDRVAEYAVELVRATRGTTLLRHGAGPRASIALLRCARALALINGAGFVTPDEVK
                     SMMLPVLRHRVILAPEVEIDGLGVDAVLGKLLEKVDAPRS"
BASE COUNT          437 a          717 c          795 g          427 t
ORIGIN      
        1 cgggcagcga taccgtgcag ggtttgtgcg ccgactggca cgcggagctg gccgatgagc
       61 agcgctaggg acacggtcaa atggctgctc ggcgcggtgc tgctcggcct gctgggctgg
      121 ctgctgtata tcaacatcga gttttacgac gaaacggagc aggccagctg gagttcaaag
      181 gcgttgagca acccctacct cgcggcgcag cagtttatgg agcgcagcgg catcgatgtt
      241 cgggatgtcc agggtctcgg ccgccagcag gagttgaata acgtcggtac gctgtttttc
      301 agcgatcccg ggcagataca gacgccccgc cagctgcaac aggtgctgga atggctgcag
      361 ggcgggggta acgtgctgta tgccgctacc gcggtcggcg acgaggacga cctgctgctt
      421 gccgaggtcg gcgtcgaggc cgcctggcgg gattatgacg acgacggcga ccagcaggag
      481 aagtcggtgg ccgagaccat gcgcgaatac aatcgacagc tcgccgaggg caagacgcgc
      541 gaacagatcg ccgagcagca ggaagaagag ggcatttcgc tgacgcgggt cgtgttcggc
      601 gacgaaatcg gcgatctcga ggtggccttt gacaacgaga tcgtgctcgc tcatgcctat
      661 gtcgatgatg acgacaccag cggagcccac cagccattca gctggtcgag ttccgactat
      721 ggtgtgcaca tgatgcagtt cgatatcggc agcgggctgc tgacaatcgt ctccgatccg
      781 caagtctgga catcctacag tatcgaccgc catgaccacg cctacctgtt atggctgctg
      841 agttccgacg gcggcgattt cgcgatgctc aggtcggtgg tgcgcgatac catctgggaa
      901 ttgatgatgc gcaacgccag cgaactgctg atcgcaggca ccctgctgct actgttatgg
      961 gtctggtttc gaggggcgcg cttcgggcgg ctgctgccac gcgaccgttc gcggcgacgt
     1021 gcgctcggcg agcacttcac ctcggtcgcg cactatctct ggcaccggcg cgacggcgct
     1081 tacctgatcg cagcactgcg ccagcaggtg ttgcgccgcg ccagcctgac attggccgga
     1141 tttgccggcg ccgacagccg gcgccagcac gaactgatcg ccgagcgctg cgacctcgaa
     1201 gcgaacgcga tcggccgcgc tctcggcgag aacaatttca acgaagccac tttcgtctcc
     1261 accgtgagac ttttaaaacg aatcgagcaa gcactatgaa cgaatccagt aacgctatcc
     1321 cggcggcgct cgccgcattg cgcgaacacg tcaacagtcg cctgatcggc caggcgcgcg
     1381 tcgtcgacga ggtgctgatc gcactgctgg ccggcggaca cgtattgatc gagggtgtgc
     1441 ccggcctcgg caagaccctg ctggtgcaga ccctggcagc gggtatcggc ggccagttca
     1501 agcgcataca gttcaccccg gacctgatgc ccggcgacgt caccggtcac gtcatgttcg
     1561 acatggcgca gagcaagttc gtgctcaagc acgggccggt attcaccaac ctgctgctcg
     1621 ccgacgagat caaccgcgcg ccggccaaga cccaggcggc gttgctcgag gtcatgcagg
     1681 agcgccaggt cacgctcgat ggccagtcga tgccggtcgg caggccgttc atggtgctgg
     1741 cgacacaaaa cccgatcgag caggaaggca cctacgcgtt gcccgaggcc gagctcgatc
     1801 ggttcatcat caagatcaat atcgactatc ccgaggcaca cgacgaggtg caaatcgcga
     1861 cgcgttacgc gctggagaat tttcagcagc aggcgagcga gatgcgctcg acgctcgatg
     1921 tcgaggccgc gcagcggcag tgcgcggcga tcgcggtcga tgaccgggtc gccgagtatg
     1981 cggtcgaact ggtgcgcgcc acacgcggta cgacgctgtt gcgacatggc gccggtccgc
     2041 gggcgagtat tgcgctgttg cgctgcgcgc gggcgctggc gctgatcaac ggcgccggtt
     2101 tcgtgacgcc cgacgaggtc aaatcgatga tgctgccggt actgcgccat cgcgtgatcc
     2161 ttgcgcccga ggtcgagatc gatggtctgg gtgtcgacgc ggtgctcggc aaattgctcg
     2221 agaaggtcga cgcgccgcgt tcatgaagcc ttcccggcta ttgctctacg cggttgccgg
     2281 ctggctggcg ctgggtgggc tggcggcgct ggcgacgatt ctcgactggc cgcgggaatt
     2341 cgaaatcaag gcgctgttct ggagttactt cgcgct
//
LOCUS       DATCZV010000316         2874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_660825, whole genome shotgun sequence.
ACCESSION   DATCZV010000316 DATCZV010000000
VERSION     DATCZV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660825"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VIW27_07100"
     CDS             complement(<1..1145)
                     /locus_tag="VIW27_07100"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY5739477.1"
                     /translation="MMVHSKIPGMRLTDLLAGFPLTDPVPAIEIHNIASNSAEVTPGS
                     AFIALPGMRSNGIDYAIDAAKAGAVAVIFDAADEYSLKRIPLLRKQVNLAWIGVGDLE
                     RANGHIVSRFFGDPGRAMTIIGITGTDGKTSVTHLVVQALTRVGKRCASIGTLGYGIA
                     NELTPDQLTTPDSVSLQTRLYQFQQQRCDYVVMEVSSHALQQYRVNGCDFDIAVLTNL
                     GRDHLDYHGSQESYAAAKARLFEDFELSGRVVNADDSFGRQLASGLERDSLIRYSVNA
                     RDEPGAEVRLESCEMTLNGQNLRVATPRGSITAVTALIGRFNVENTLACIATLVALGL
                     DRNQLELAVKDLHSIPGRMEKFAGEAGMPTVVIDFAHTEQALRACLEA"
     gene            complement(1142..>2874)
                     /locus_tag="VIW27_07105"
     CDS             complement(1142..>2874)
                     /locus_tag="VIW27_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5739478.1"
                     /translation="MKRGDPTQEYRPIFLATRHYSALLLLLALLAGLVVRALYLQLVE
                     QDFLASQGVQRQIRTIETPAYRGAILDRFGTPLAISTPVDSVWVNPGEILENLGALKR
                     VTASLDLDYRDTVAMLKQRADREFVYLRRQLEPEFAASVANGVDGVYLQREYHRFYPA
                     GEVVSHLVGFTDIDDHGQEGLELAYDDWLGAQPGERRVIRNRFGEVVEELAQVKAARS
                     GNDIHTSIDMRLQYIAYRSLARAIQYHAAAAGSAVLLDARSGEILAMVNQPSYNPNSR
                     NSMAPGQLRNRALTDVFEPGSAIKPFTFAAAMERGRFDSGSRIDTSPGYLMVSGHPIK
                     DTRDLGSLSLADILRQSSNVGATRVALSLQRDELWQSFREFGFGSVSGVSFPGESVGY
                     LRHYSQWQPLDHATMGFGYGMSVSISQLARAYAMIANLGRLPELSLLRKDPDSPRDNQ
                     ISRHVIKSSTARQLLQMMSEVVGPGGTAQQAAVPGYRIAGKTGTARKSVDGGYRQDDY
                     VAVFAGIAPASNPRLVMAVMIDEPTQNGYYGGVVAAPVFQEVISNALRILDIPPDDLP
                     ALAQSRREGA"
BASE COUNT          509 a          920 c          925 g          520 t
ORIGIN      
        1 gcttcgagac aggcgcgaag tgcctgctcg gtatgcgcga aatcgattac caccgttggc
       61 atacctgcct caccggcaaa tttttccatg cgccccggta tcgaatgcag gtccttgacc
      121 gccagctcga gctggttgcg gtcgaggccg agcgcgacca gcgtggcgat gcaggcgagc
      181 gtattctcga cattgaaacg cccgatcaac gcggtgaccg cggtgatact gccacgcggc
      241 gtggcgaccc tcaggttctg gccgttgagc gtcatttcgc aggactcgag ccgcacctcc
      301 gcgccgggct cgtcgcgcgc attgaccgag tagcggatca acgaatcccg ctccagtccg
      361 ctggccagct gccgcccgaa actgtcgtcg gcattgacca cccggccgga aagttcgaag
      421 tcctcgaaca ggcgcgcctt ggcggcggca tagctttcct ggctgccatg gtaatcgagg
      481 tgatcgcggc caaggttggt cagcaccgcg atatcgaagt cgcagccgtt gacccggtat
      541 tgctgcagcg catgcgacga aacttccatg accacgtagt cgcagcgctg ttgctggaac
      601 tggtaaaggc gggtctgcag gctgacgctg tccggggtcg tcagctggtc gggagtcaat
      661 tcgttggcga taccgtagcc gagcgtaccg atactggcgc agcgcttgcc gacgcgagtc
      721 agcgcctgta ccaccaggtg ggtgacgctg gtcttgccgt cggtgccggt gataccgatg
      781 atcgtcatcg cgcggcccgg gtcaccgaag aagcgactga cgatatgacc attggcacgc
      841 tccaggtcgc cgaccccgat ccaggccagg ttcacctgct tgcgcaacag cggaatgcgc
      901 ttcaggctgt actcgtcggc ggcatcgaag attaccgcga cggcaccggc cttggcggcg
      961 tcgatcgcat agtcgatgcc gttgctgcgc atgcccggca gcgcgatgaa tgccgaaccc
     1021 ggtgtgacct ccgcgctgtt gctggcaatg ttgtgaatct cgatcgccgg caccgggtcg
     1081 gtcagcggaa aaccggccag tagatccgtc agccgcatac cgggaatttt gctgtgaacc
     1141 atcatgctcc ctccctcctg gattgtgcca atgccggcag gtcatccggc ggtatatcga
     1201 gaattcgcag tgcgttcgat atcacttcct ggaataccgg tgcggcgacc acgccgccgt
     1261 aataaccgtt ttgcgtgggc tcatcgatca tcaccgccat caccagccgc gggttgctgg
     1321 ccggcgcgat accggcgaac accgccacgt aatcgtcttg acggtaaccg ccgtcgacgc
     1381 ttttcctggc ggtaccggtc ttgccggcga tgcgatagcc gggcaccgcg gcctgctgcg
     1441 cggtgccgcc cggaccgacg acttcgctca tcatttgcag caactggcgc gcggtcgaag
     1501 acttgatcac gtggcgcgat atctggttgt cccgaggcga atcgggatcc ttgcgcagca
     1561 ggctgagctc gggcagccgg ccgagattgg cgatcatcgc gtaggcgcgc gccagctggc
     1621 tgatcgacac cgacatgccg tagccgaagc ccatggtggc gtgatcgagc ggttgccact
     1681 ggctgtaatg gcgcaggtac ccgaccgact cgccggggaa cgacaccccc gagacgctgc
     1741 cgaaaccgaa ttccctgaaa ctctgccaga gctcgtcgcg ctgcagcgac agggccacgc
     1801 gcgtggcacc gacgttgctc gactggcgca ggatatcggc gaggctcagg ctgccgaggt
     1861 cgcgggtatc cttgatcggg tgaccactga ccatcaggta tcccggcgag gtgtcgatgc
     1921 ggctgccact gtcgaaacgc ccgcgttcca tcgccgccgc gaaggtaaac ggcttgatcg
     1981 ccgaacccgg ctcgaagaca tcggtcagcg cgcggttgcg caactgtccg ggggccatcg
     2041 aattgcggct gttcgggttg taggacggct ggttgaccat cgccagaatc tcgccgctac
     2101 gcgcatcgag cagcaccgcc gaacccgccg cggcggcatg gtactggatg gcccgcgcca
     2161 ggctgcggta agcgatgtac tgcaggcgca tgtcgatgct ggtatgaata tcgttgccgg
     2221 agcgcgccgc cttgacctgc gccagctcct cgacgacctc gccaaagcgg ttcctgatca
     2281 cgcgccgttc gccgggctgc gcgccgagcc agtcatcgta ggcaagttcc aggccttcct
     2341 gcccatgatc gtcgatatcg gtaaagccga ccaggtggga gacgacctcg ccagccgggt
     2401 agaagcgatg atattcgcgc tgcaggtaga cgccgtcgac accgttggcc accgaagccg
     2461 cgaactccgg ctccagctgg cgcctgaggt agacgaattc gcggtcggcg cgctgcttga
     2521 gcatcgccac cgtgtcgcgg taatcgaggt ccagactggc ggtgactcgc ttcagcgcac
     2581 cgaggttttc aagaatctcg cccggattga cccacaccga gtcgaccggt gtgctgatcg
     2641 ccagcggggt gccgaaacgg tcgagtatcg cgccgcggta ggccggcgtc tcgatggtgc
     2701 ggatctgccg ctgcacgccc tggctggcga gaaaatcctg ttccaccagc tgcaggtaaa
     2761 gggcgcgcac caccagcccg gcgagcaacg ccagcagcag cagcaacgcg ctgtagtgcc
     2821 gcgtggcgag aaaaatcggc cggtactcct gggttggatc gccgcgcttc atcg
//
LOCUS       DATCZV010000317         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_607945, whole genome shotgun sequence.
ACCESSION   DATCZV010000317 DATCZV010000000
VERSION     DATCZV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607945"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            695..958
                     /locus_tag="VIW27_07110"
     CDS             695..958
                     /locus_tag="VIW27_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739479.1"
                     /translation="MALGLQYDPVGSSGDNSSYRDAYLQLAYQYGIDPLVCGIGAVVY
                     LENVGNGGLSGWGLSYTYLQIEEQQVAKTFDASRAELYYSWRF"
     gene            1478..2254
                     /locus_tag="VIW27_07115"
     CDS             1478..2254
                     /locus_tag="VIW27_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739480.1"
                     /translation="MPLPGSTSGSIQFPSSAQPAESDNLLLSGRIEQGVDVLSISANT
                     AVNLFARVEYKVDTEEFDFNRKILTAVGFKLRHYFSDTFVMSVGAKYENDKRFVVERS
                     SDGTLLFGNWFGSWQLPKDDFGEPAGRRPLAYPGLTWGELRFPGSQDPIEESSMVLEG
                     YVEQGVDWSNWERWGIFNFYGNLDFIADTEEIEWNNMLAVGAGIKLKKRVGNNLLLQY
                     GFEATREHYWVTDETVDVIFAYLNWSAWWDPRAIRRDLPE"
BASE COUNT          579 a          769 c          852 g          580 t
ORIGIN      
        1 tcctttcgaa tccaccggtt agcggtttgg cgccgagtac cggcggggat tccgctgcgg
       61 cgggacccac gagtcacgac caatcgagtg gcgcgatcca gggcggggcg aaagtcggcg
      121 gcacgaaatg gaaagtaaaa cacgacgata ccctgtactc gatcagtcgg cgggtgtttc
      181 ctcgggatac cggccagcag gcgcgcctga ggcaggatat cgtggtgctg aatcccgccg
      241 tgttcgtcaa cggcgcgaac gagatggctg tcggtgccgt gctcgagctt tccgcttaca
      301 tcgttatcga caatagcgcg accagttcag ttcagccggc gccagcggct cggttggcag
      361 cggcggagat tgcctccagc atgacgccag catcggtgaa tgcgggtccg cgggtcgagc
      421 ctgcgacggt gagggcgagc caggcggcct cgacttcgaa gccagcagcg cgagcagcat
      481 cgaaaacccc gggtaaggac gaatccgttg cgagcggaag ccagtcgagc gcggccgtga
      541 atgcagcgcg taccagtgcc tcggtgagcc tgggttacgc gagcggtggg gataaactgg
      601 tcgatctgga tggtggcaac gaccctacgc gggatcgggt gtacacatgc gcctcggttt
      661 cgagcaaata tcccggcgag gcggcggcta tcgcgtggcc ctcggactgc agtacgatcc
      721 ggttggcagc agcggtgata actcgagcta cagggacgcg tatctgcagc tggcatacca
      781 atacgggatc gatcccctgg tctgcggtat cggcgcggtc gtatacctgg aaaatgtcgg
      841 caatggcgga ctgtcgggct ggggcctcag ttacacgtat ctgcaaatcg aggaacagca
      901 ggtcgcaaaa accttcgatg cctcgcgagc cgagctttat tacagctgga ggttttaatt
      961 tctgttcacg gcccgacgcc gacgggcaat gatcgcttgc ctggttatcg tttcccgagc
     1021 cgataacagt aagcggagtc ggtgccggaa tgaagcctgg ccactgctat ccccgcttgg
     1081 tgtccgggaa ctggctgcgg ccgtggttcg tcgatacgcg gccgggaaaa tgatgcgaca
     1141 gctatcgtct ccattgaccg cggcggtttc ttgcactaat tatgaaggta attcgcctgc
     1201 tggcgtcgac cggcgtcgat gcttttatcg attacgcttt cgctggacag acgtctgacc
     1261 ctgatattag ttgcctgcag cagagctagt cgtgaccgat ttcaagtccg gtgttcggca
     1321 aagtctataa aatcgggcaa tcccgagtga tatgaatgac tcttagatgc aattcagatc
     1381 tttctgcagc ctgagtaaac gcatgccagt agcactggta ttgatgctat cggccatagt
     1441 ctacctggtc gcgacgcccg ccagcgcgca aagcgccatg ccgctgccgg gatcgacctc
     1501 cgggagtatc cagtttccga gttccgcgca gcccgctgaa agcgataacc tgctgcttag
     1561 tggcaggatc gaacaggggg tggacgtgtt gtcgatttcc gcaaatacgg ccgtcaatct
     1621 gtttgcccgc gtcgaataca aggtggatac cgaggagttc gacttcaaca ggaaaatcct
     1681 tacggcggtc gggtttaaac tgcgccatta tttttccgat acgttcgtca tgtcggtcgg
     1741 agcgaagtac gaaaacgaca agcgctttgt cgtcgagagg agctctgacg gtacgctgct
     1801 gtttggcaac tggtttggtt cctggcaact tcccaaagat gattttggcg agccggccgg
     1861 gcgacggccg ctggcctatc cgggcctgac atggggcgag ctcagattcc ccgggtccca
     1921 ggatccgatc gaggagagtt cgatggtgct cgaaggctac gtggaacagg gcgtcgactg
     1981 gagtaattgg gagcgctggg ggatcttcaa tttttacggc aacctcgact tcattgccga
     2041 caccgaggag atcgaatgga acaacatgct cgccgtcggc gccgggatca agctgaaaaa
     2101 acgcgtcggt aataacttgt tgttacagta tggattcgaa gcgacacggg aacactactg
     2161 ggttaccgac gaaaccgtgg acgtgatttt cgcctatttg aactggtcgg cctggtggga
     2221 tccgcgggcg attcggcgcg acctccccga ataatgacat caacagcgca ggcgagaaaa
     2281 gtccctgcga caggtcacgg gtcgagccag cgatgcgacg gcaggtccgg ctcatttcaa
     2341 aactctcgaa cctcgagata aatccacaaa ttcggctgga gaacagcggc ctgccgcggt
     2401 tcgtcgatcg ttcagcctat cactgcccgt actccgatta tatcgaggcc gcctggccgt
     2461 ccgtcaccct tgctgcccgg ccctccacta tagccattgc ccgtgccggc ttacagcggc
     2521 tcgcaccacg acctggccgt tcgacacgct tcggctgacc tgcgttctcg cctggttgcg
     2581 ggaagctgac cggcctcaca aagactcgtc acgtttcacg atccgggggc ctcgctactg
     2641 ccaggacctg ccacgaaaag cgcgtgaaac ctgtacttta gggcagttca aaactggaca
     2701 ttcgagagga acttttcgtc agccggtgtc gttttacgga taaacgggca gtaatcagca
     2761 agctggtaaa aatcaattta
//
LOCUS       DATCZV010000318         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_395941, whole genome shotgun sequence.
ACCESSION   DATCZV010000318 DATCZV010000000
VERSION     DATCZV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395941"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIW27_07120"
     CDS             complement(<1..260)
                     /locus_tag="VIW27_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019829115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5739481.1"
                     /translation="MTTRRNQLRNIAIIAHVDHGKTTLVDQLLRQSGSLDSRGDVSER
                     VMDSNELEKERGITILSKNTALNWHHPGRGQDYRINIVDTPG"
     gene            complement(440..907)
                     /gene="bcp"
                     /locus_tag="VIW27_07125"
     CDS             complement(440..907)
                     /gene="bcp"
                     /locus_tag="VIW27_07125"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY5739482.1"
                     /translation="MLEVNQTAPDFSVVDQDGEAVSLAQFQGDKHVVLYFYPKDDTPG
                     CTTEANEFTALIDQFAAAGAVVLGVSKDSCEKHRKFIAKHDLKVRLLADTDASLCELY
                     GTWGPRKFMGREYMGIGRSTFVIDKQGKLAHVDYKVKAKGHARAILEVVEGLA"
     gene            complement(1012..1560)
                     /locus_tag="VIW27_07130"
     CDS             complement(1012..1560)
                     /locus_tag="VIW27_07130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEY5739483.1"
                     /translation="MTQGLIIVDVQNDYFAGGSMELVAMEEAAANCRALLDAFRADSA
                     PLFHIQHLAIREGATFFVPGTPGAEIHHSMTPAAGETVFVKHYPSAFRDTCLHESLQL
                     GGIDELVICGAMSHMCIDTTVRAAFDLGYRCRVAADACATRDLEFGGRRVAAADVHAA
                     FMGALAVPFAAVLPSAQILDAR"
     gene            complement(1704..2150)
                     /locus_tag="VIW27_07135"
     CDS             complement(1704..2150)
                     /locus_tag="VIW27_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739484.1"
                     /translation="MNKKTLIAAATLWLQASIATLSQAAAASHPFEFHADATMVHERM
                     GEIENAYGYMNVITDGEGLGTINVMFSNGSELERAQFNARVRFLNTGGKVLQEKNFDC
                     WIDAENLREAGECKVSQPLTVRDFDAVEVDFYLTDIPELSAALISY"
BASE COUNT          432 a          698 c          744 g          479 t
ORIGIN      
        1 tgtcccgggg tgtcgacgat gttgatgcgg taatcctggc cgcgcccggg gtgatgccag
       61 ttgagcgcgg tattcttcga caggatggtg atgccgcgct ccttttccag ttcgttggaa
      121 tccatcaccc gttcgctgac atcgccgcgg ctgtcgaggc tacccgactg gcgcagcaac
      181 tggtcgacca gcgtggtctt gccgtggtcg acatgcgcga tgatcgcgat gttgcggagc
      241 tggttgcgtc gggtggtcat aggtggcgag ccgccgggaa agtcgaggcg cgcgattata
      301 accgcttgcc ggttttccac aagtgtccgc gtcacaggtt tgcgaaccgc gtcgtggcgt
      361 cgtcgatagc cgggccggaa tcctctagcc tgccgccgtt acaggatccc cgtttgcgcg
      421 gggatggttg agggactcgc taggcgagtc cctcaaccac ttcgaggatc gcccgggcgt
      481 ggcccttggc cttgaccttg taatcgacgt gggccaactt gccctgcttg tcgatgacga
      541 aggtggagcg gccgatgccc atgtattcgc ggcccatgaa tttgcgcggg ccccaggtgc
      601 cgtagagttc gcacagcgag gcgtcggtat cggcgagcag gcgcactttc agatcgtgct
      661 tggcgatgaa cttgcggtgt ttctcgcaac tgtccttgct gactccgagg accacggcgc
      721 cggcggccgc gaactggtcg atcagcgcgg tgaactcgtt ggcctcggtg gtgcagccgg
      781 gtgtgtcgtc cttcgggtag aagtaaagca cgacgtgctt gtcgccctgg aactgcgcga
      841 ggctaaccgc ctcgccgtcc tggtcgacca cgctgaaatc gggtgcggtc tggtttactt
      901 cgagcattgg cttgatccgt tatcgatgaa agcgcaatca taaaagagcc cgccgcaaac
      961 ttcaaaacgc tgctgcggag ccggtggcaa tagccgctgc gaaacgcgca gctagcgcgc
     1021 gtcgagtatc tgcgcgctcg gcaggactgc tgcgaaaggt acggccagcg cacccatgaa
     1081 agccgcgtgt acgtcggcgg cggcgacccg gcggccaccg aattcgaggt cgcgcgtggc
     1141 gcaggcgtcg gcggcgaccc ggcagcggta accgagatcg aaggccgcgc gcaccgtggt
     1201 atcgatgcac atgtggctca ttgccccgca gatcacgagt tcgtcgatgc cgccgagctg
     1261 tagtgactcg tgcagacagg tgtcacgaaa cgcactcgga taatgcttga cgaataccgt
     1321 ctcgccggcg gcgggtgtca tgctgtggtg aatttcggcg cccggcgtgc cgggtacgaa
     1381 aaaagtcgca ccttcgcgga tcgccaggtg ctgaatatgg aacaggggcg cggaatcggc
     1441 gcgaaacgca tcgagcagtg cgcggcagtt agcggcggct tcctccatcg ctaccagctc
     1501 catcgagccg ccggcgaagt aatcattctg cacgtcgacg ataatcaggc cctgggtcat
     1561 gccttcggtc tccgctgttg gaatcgggga aaagacgcct tgcgggatcc acggcgaagc
     1621 ggcgaagatc gcgccaggcg tcgttgcccg gccactctaa cagatcacct tcgggtgccg
     1681 ccagcccgtg ccgccgctgg tgatcaatag gatatcagcg cggcgctgag ctcgggaata
     1741 tcggtcaggt agaaatcgac ctcgaccgca tcgaagtcgc gcacggtcag cggctgggat
     1801 accttgcatt ctccggcttc gcgcagattc tcggcatcga tccagcagtc gaaatttttt
     1861 tcctgcagca ccttgccgcc ggtgttgagg aacctgaccc gcgcattgaa ctgcgcgcgc
     1921 tccagctcgc tgccgttcga gaacatcacg ttgatcgtac cgagaccttc gccgtcggtg
     1981 atcacgttca tgtagccata ggcattttcg atctctccca tgcgttcgtg aaccatggtc
     2041 gcatcggcgt gaaactcgaa cggatgcgaa gccgctgcgg cctgcgacag cgtcgctatc
     2101 gaagcctgca gccacagggt tgctgccgcg atcagagttt ttttgttcat ctcgtcaacc
     2161 tcttcggtcc tttgatttca gtcaacggga tttatacagc cgcgctgtga aaattcggtg
     2221 aaaagcggga aatagtgcaa aatccgtgat cgaatcgaaa ttcagcccgg gtgcggcatc
     2281 tgttagcata ggcggcgcca aacatcctca aaccgtctgc cgaagagccc acccatgttc
     2341 gacgacaagc ccc
//
LOCUS       DATCZV010000319         5348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_713708, whole genome shotgun sequence.
ACCESSION   DATCZV010000319 DATCZV010000000
VERSION     DATCZV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713708"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..727
                     /locus_tag="VIW27_07140"
     CDS             107..727
                     /locus_tag="VIW27_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEY5739485.1"
                     /translation="MTKPLAANRALQLMLLWLLAVWVITAIEPLYPRDWLLENLLVFA
                     TAAVLIPGYRRFRFSNFSYALLTIFLTLHMAGAHYTYAETPLGFWLQDWFGFERNHYD
                     RIVHFSFGLLLVLPLREYLERRSGAGPGWCYLLAVICVMALSAAYEIIEMLAAVVVSP
                     ELGAAYLGTQGDEWDAQKDSALATAGAILAAVLAWGWQRLYSHKTS"
     gene            complement(770..1162)
                     /locus_tag="VIW27_07145"
     CDS             complement(770..1162)
                     /locus_tag="VIW27_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739486.1"
                     /translation="MSFSRLAALGRPRAWSHGIGHAPGDAWSTRLASHEKSCVRIGNP
                     KRAIRVCQLGSDQAMWLINISFQGKIARKQAIKVCFWRQNKRFNCFGFFTTGNYFHLE
                     FHAEKGLYQGIKGRFFRFGYKNQKLILV"
     gene            complement(1164..1685)
                     /gene="moaB"
                     /locus_tag="VIW27_07150"
     CDS             complement(1164..1685)
                     /gene="moaB"
                     /locus_tag="VIW27_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEY5739487.1"
                     /translation="MSETREFIPLRIAVLTVSDSRDESSDKSGALLVRFVTEAGHGVA
                     EKTIVPDDKYRIREVLSRWIADDTVQVVLTTGGTGVTGRDGTPEAVAPLLDKIIDGFG
                     ETFRSLSYQSIGTSTLQSRAIAGVANGTYVFCLPGSSGACKDAWTQLISTQLDYRTRP
                     CNLVELMPRLRET"
     gene            complement(1691..1891)
                     /locus_tag="VIW27_07155"
     CDS             complement(1691..1891)
                     /locus_tag="VIW27_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEY5739488.1"
                     /translation="MDEEALNMSIRKFLKKVGITSQREIERAIDQANAEGRLDGNPVR
                     VKVTLECDLIDDALSIEDEIRY"
     gene            2051..2311
                     /locus_tag="VIW27_07160"
     CDS             2051..2311
                     /locus_tag="VIW27_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739489.1"
                     /translation="MLVPILTDRGAWLALGGRLQIGLLLAADPSLSNPYGIIAVNPAR
                     HDQVNCAATRRIDRSDHPAQHRIAEYRIDGQALFEPVAKWAQ"
     gene            2462..3583
                     /gene="mobA"
                     /locus_tag="VIW27_07165"
     CDS             2462..3583
                     /gene="mobA"
                     /locus_tag="VIW27_07165"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY5739490.1"
                     /translation="MSLRREKITAAILAGGKGRRMGGQDKGLLELDGKPLVAILIDSL
                     RRQGVDIVINANRNREQYAQFGYPVIADRLEDYQGPLAGFASVLHAVDSGFVLTLPCD
                     GPLIADDYVERFIDCQAKSGAPLCVAFDGERLQPVHALIDTALRDDLDRFLDVGERKI
                     DRWYANYDFARADFADCKQMFRNINTPADRRALQSGRLKTDIPILGFSAWSGTGKTTL
                     LRQLIPALAARDLRVSVIKHAHHHFDLDVPGKDSYELRKAGAAQTVICTATRMAQITE
                     FDSPADEPGLEQIINGLDTGRIDLVLVEGYKTIEFPKIELHRAEIGKPCLYPEDDSVI
                     ALACDTPPADDIDIPVLDINDVAAIADFICNDFYPPRKT"
     gene            3764..4246
                     /locus_tag="VIW27_07170"
     CDS             3764..4246
                     /locus_tag="VIW27_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5739491.1"
                     /translation="MHKWVDDDGVTHYSDEAPPSVVTTLIELPEPPRADSAGSSGEPQ
                     DDYYSISNQWQRMHQERLERERLNLERERIRVARQAPPAAAKDDDDAVRYLPVYSGFG
                     YYRPHRRHHRYHGRRRNPVSAPFPRNKSIRPVAVPRQLMASSALLTLTIPARRVTLRR
                     "
     gene            4243..>5348
                     /locus_tag="VIW27_07175"
     CDS             4243..>5348
                     /locus_tag="VIW27_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5739492.1"
                     /translation="MKTTKSKWLIAATLALALACPTATVQGAQPYLSLPVGEDTDVDI
                     IRYTAEGDYLLLWLAPEYGFREAHRQLARRLPAEGIEVWQTSLAEALFMPNGAATMRQ
                     LDGSLVADLIAATHAASGKRILLAGDSYATLPVLSGAHHWQQRHDADYLLGAVLFTPY
                     AYASIPPLGQEPEYMPIVDATNIPLLVFQAQNSATRHRFDELLERLRRHGSPVYTRMI
                     PDLMSLFYEEPPSAAMRDGSAPIPRSLRQLLPLLQQHSVPRDPVPMRSAAATDGGIDI
                     YLREFTADLRPLPLDLDDIDGRRVVKTDFTGQVTMINFWASWCGPCVEEIPSLNRLQQ
                     KMAGKPFELISINFAEDRQTVAAFMERVRVDFPVL"
BASE COUNT         1026 a         1732 c         1577 g         1013 t
ORIGIN      
        1 ggcgagacga tcgatttcga cgacgtgccg ccaccgcgca caccgcgccc ggaccacgat
       61 acgcgccgct ccgccgacta gcgccgccgc cgagcatgca gccccgatga cgaaacccct
      121 ggcggccaac cgcgccctgc aactgatgct gctgtggctg ttggcggtat gggtgatcac
      181 tgctatcgaa cccctctacc cgcgcgactg gttactcgag aacctgctgg tcttcgccac
      241 cgcagcggtc ctgatcccgg gctaccgccg gtttcgcttt tccaacttca gttacgccct
      301 gctgacgatt ttcctgaccc tgcacatggc cggcgcccac tacacctatg ccgaaacccc
      361 gctcggcttc tggctgcagg actggtttgg tttcgagcgc aaccactacg accgtatcgt
      421 gcatttctcg ttcggcctgc tgctcgtcct gccgctgcgc gaatacctgg agcgccgctc
      481 cggcgccggt cccggctggt gttatctgct ggccgtgatc tgcgtgatgg cgctgagcgc
      541 cgcctacgaa atcatcgaga tgctcgccgc cgtcgtcgtc agccccgagc tcggcgccgc
      601 ctacctggga acccagggcg acgaatggga cgcgcagaag gactccgcgc tggccaccgc
      661 cggcgcgatc ctcgcggccg tcctcgcttg gggatggcag cgcttataca gccacaaaac
      721 cagctaaggt gatatcggat cccacagcca ggcaccaggt ctgagagggt cataccaaga
      781 taagcttctg atttttataa ccaaaccgga aaaatcggcc ctttatcccc tgataaaggc
      841 ctttttcagc gtgaaattcg aggtgaaagt aattgcctgt agtaaaaaaa ccaaagcaat
      901 taaaacgttt gttttggcgc caaaaacaca cttttatcgc ctgtttacgt gcgatttttc
      961 cttgaaagct gatatttatc agccacatgg cttggtccga ccctaattga cagaccctaa
     1021 ttgctcgctt tgggttgcct atcctgacgc aggatttttc gtggctggca aggcgggtag
     1081 accacgcgtc gccgggagca tggcctatgc catgcgacca agcgcgtggc ctgcccaacg
     1141 ccgccaggcg cgaaaaagac aagtcaggtt tcgcgcaggc gcggcatcag ttcgaccagg
     1201 ttgcaggggc gcgtacggta atcgagctgc gtactgatta attgcgtcca cgcatccttg
     1261 cacgctccgg aagatcccgg cagacagaat acgtaagtgc cgttggcgac accggcaatc
     1321 gcgcgcgatt gcagcgtcga ggtgccgata ctctgatagg acaggctgcg aaaggtttcg
     1381 ccaaagccgt cgatgatctt gtcgagcagg ggtgccacgg cttccggcgt accgtcgcgg
     1441 ccggtgacgc cggtgccgcc ggtggtcagc accacctgca ccgtgtcgtc ggcgatccag
     1501 cgcgacaaca cctcgcgaat gcgatacttg tcgtcgggca cgatggtttt ctcggcgacg
     1561 ccgtggccag cctcggtgac gaagcggacc agcaaggccc cggacttgtc actgctttcg
     1621 tcgcgcgagt cggataccgt cagcacggcg atccgcagcg gaatgaattc tcgcgtctcg
     1681 ctcatcgtct ttagtagcga atttcgtctt cgatcgacag cgcatcgtcg atcaggtcgc
     1741 attccagcgt caccttgacg cgcaccgggt tgccgtcgag ccggccctcg gcattcgcct
     1801 ggtcgatggc gcgctcgatc tcgcgctgcg aggtgatacc cacctttttc agaaacttgc
     1861 ggatcgacat gttgagcgct tcttcgtcca tcggtttgcc tcttgtgaaa gttcggagtt
     1921 cgcattttac ccgttttggg cgggtgcccc aacgaatatt cgacgcggtt gcagacggct
     1981 tgtcaggctt gctcctgccg aacctcgtct gaccgtttca gcagccttga ctcgaacacc
     2041 gtttaccgca ttgctggttc caatactgac cgatcgcggc gcctggctgg cgctgggcgg
     2101 tcgcctgcag atcggcctgt tgttggcagc cgatccgtcg ctgtcgaatc cttacggtat
     2161 catcgccgtc aatccggcgc gccacgatca ggtcaactgc gcggccaccc gtcgaattga
     2221 tcgctcggat cacccggcgc agcacaggat cgcggagtat cgcatcgatg gccaggcact
     2281 gttcgagccg gtcgcgaagt gggcccagta acgcgcggca aattcgaaac cgcggctttt
     2341 ttgcgccttc gcctgttgca gtattttcgg ggtgagtgca gtatccgcgg atgatcgaaa
     2401 ttatcggatt tttcccagtg aatctcggca agcgtaacgg ctcaggccgg cccgggcagc
     2461 gatgagcctg cgccgtgaga agattaccgc ggcgattctc gccggcggca agggccgtcg
     2521 catgggcggc caggacaagg gcctgctcga gctcgatggc aagccgctgg tcgccatcct
     2581 gatcgacagc ctgcggcggc agggcgtcga tatcgtcatc aacgccaacc gcaatcgcga
     2641 gcagtacgcg caattcggtt acccggtgat cgccgatcgg ctcgaggatt accaggggcc
     2701 gctggccggc ttcgccagcg tgctacatgc cgtcgacagc ggcttcgtac tgaccctgcc
     2761 ctgcgacggc ccgctgatcg ccgatgacta tgtcgagcgc ttcatcgact gccaggcgaa
     2821 gagcggggca ccgctgtgcg tggccttcga cggcgagcgc ctgcaaccag tgcacgcact
     2881 gatcgatacc gcgctgcgcg acgacctgga tcggttcctc gatgtcggcg agcgcaagat
     2941 cgaccgctgg tacgcgaact acgatttcgc gcgcgccgat tttgccgatt gcaagcaaat
     3001 gttccgcaat atcaacaccc cggccgaccg ccgcgcgctg cagtccgggc ggctaaagac
     3061 cgacatcccg attctcggtt tcagcgcctg gagcggcacc ggcaagacca cactgcttag
     3121 gcaattgatt ccggcgctcg ccgcgcgcga cctgcgtgtt tcggttatca agcatgctca
     3181 ccaccatttc gatctcgacg tacccggcaa ggacagttac gaactgcgca aggcgggcgc
     3241 ggcgcagacc gtgatctgca ccgcgacgcg catggcgcaa atcaccgagt tcgactcgcc
     3301 cgccgatgaa ccggggctcg agcagataat caacggcctg gataccgggc gcatcgacct
     3361 ggtgctggtc gagggctaca agacaatcga gtttccgaag atcgaattgc atcgtgccga
     3421 gatcggcaag ccctgccttt accccgagga cgactcagta atcgcgctgg cctgcgatac
     3481 gccaccggcg gatgacatcg atatcccggt actcgacatc aatgacgtcg cagccattgc
     3541 agacttcatt tgcaacgatt tttacccgcc acgcaagact tgacgacagc gcgcgaacca
     3601 ccgccggagg gattttcggg caggcggcgc aaattccgtg gataatagcc gcatcgcgat
     3661 aacggtcgca cggcaattga cggatcgacg acggcatgaa tcggcattgc acggcatcac
     3721 tgggactggc cctggtcttc ggcagcctgc agacggcggc ccggtgcaca agtgggtcga
     3781 cgatgacggc gtcacgcact actcggacga agcgccgcca agcgtggtaa ccacgttgat
     3841 cgaactgccg gaaccgccgc gcgccgactc cgctggcagc agcggcgagc cgcaagacga
     3901 ttattactcg atttcgaatc aatggcagcg tatgcaccag gagcgcctcg agcgcgaacg
     3961 gctgaacctt gaacgggaga gaattcgtgt cgcgcgacag gcaccgccgg cggcagccaa
     4021 ggacgatgac gacgcagtcc gttacctccc ggtctactcc ggattcggct actaccggcc
     4081 tcaccgccga catcaccgct atcacggccg ccggcgcaat ccagtctcgg cacctttccc
     4141 acgcaataaa tcgatccggc cggttgccgt gccgcggcag ctgatggcat catcagcatt
     4201 gctaaccctg acaatacccg ccagacgcgt gactttgcgg cgatgaaaac caccaaatcc
     4261 aaatggctga tagcggccac gctggcgctg gccctggcct gcccaacagc aaccgtgcag
     4321 ggcgcgcaac cgtatctcag cctgccggtc ggcgaagata ccgacgtcga tatcattcgc
     4381 tacaccgccg aaggcgacta cctgttgcta tggctggcgc ccgaatacgg ttttcgcgag
     4441 gcgcatcgac aactggcgcg tcgcctgcct gccgagggta tcgaggtatg gcagaccagc
     4501 ctcgccgagg cgctgttcat gcccaacggc gccgccacga tgcgccagct cgacggcagc
     4561 ctggtcgccg atctgatcgc cgcaacccac gccgcgagcg gtaagcgaat actgctcgcc
     4621 ggtgacagtt acgcgacgct gccggtgctg tcaggcgccc accattggca gcaacgtcac
     4681 gacgccgact acctgcttgg cgcggtactg ttcacaccct atgcctatgc gtcgattcca
     4741 ccgctcgggc aagaacccga atacatgccg atcgtcgatg cgaccaatat tccgctgctg
     4801 gttttccagg cgcagaacag tgccacccgg caccgtttcg acgagttgct cgagcgcctg
     4861 cgccggcacg gcagcccggt atacacgcgc atgataccgg acctcatgag cctgttttac
     4921 gaggaaccac cgagcgcggc gatgcgcgac ggcagcgcgc cgattccgcg cagcctgcgg
     4981 caattactgc cgctgctcca gcagcactcg gtgccgcgcg acccggtgcc gatgcgcagc
     5041 gccgcggcaa ccgacggcgg catcgacatt tacctgcggg aatttaccgc cgacctgcgt
     5101 ccgctgccgc tggacctcga cgatatcgat ggcaggcggg tggtaaaaac cgacttcacc
     5161 ggccaggtca cgatgatcaa tttctgggcc agctggtgcg gaccctgcgt cgaggaaatc
     5221 ccgtcgctga accggctgca gcaaaaaatg gccggcaagc cattcgaact gatatcgatc
     5281 aatttcgccg aggaccggca aacggtggct gccttcatgg agcgggtccg ggtcgatttc
     5341 ccggtact
//
LOCUS       DATCZV010000320         2698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_25905, whole genome shotgun sequence.
ACCESSION   DATCZV010000320 DATCZV010000000
VERSION     DATCZV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25905"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VIW27_07180"
     CDS             <1..1470
                     /locus_tag="VIW27_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5739493.1"
                     /translation="LRNRFRPLEALDEEQLEFIHQVSLRIVEEQGIEVIGDLALDLFR
                     NAGAEVDADGVVRIDRALLLETVGRAPADFEVRTRNPEQALAVGGDVINFGLVSGTPN
                     VHDNINGRRAGNFEDYRKLISFGQYFNVLTFFGNQATAPTDLPANSRHLDTTYINLTL
                     SDKAFFATGIGGGRARDAIEMSAIARGLTMEEMKTHPSVMTNININSPRKLDDSMAYG
                     AMQMALFGQPVTVTPFTLMGAMTPATMAGALAQQNAEALVGIALLQLTRPGTPCVYGG
                     FTSNVDMRSGSPAFGTPENSLANIAGGQLARKYGLPYRSSACNASNAVDAQATYETLM
                     ALWSSVMGHGNLIYHTAGWLEGGLVASFEKLIIDCEMLQHLSKMLEPLKIDLDEVGLE
                     AMREVGPGGHFFGCAHTMERYRNAFYEPFVSDWQNSENWVAAGSKDATARATELWPKI
                     LEEFTPPPVDAAVCEALETYVARRKEELGGEEPGLEPTD"
     gene            complement(1510..>2698)
                     /locus_tag="VIW27_07185"
     CDS             complement(1510..>2698)
                     /locus_tag="VIW27_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5739494.1"
                     /translation="IPQLDTIRQFRLQRLVQELQRNDYGGLLLYDPLNIRYATDSTNM
                     QLWITHNPARACFVSADGYIVLFDFHGGDHLSTHLPLVREVRGGASFFYFESGTRTDE
                     HAAKFAAQIDDLMRTHAGANRRLAVDKIEIAGLRALEKLGLEVFEGQAVTEIARVIKC
                     DDEIRAMRCAIAACEAAMYEMQDAVAAGKSENEIWSVLHAGNVKRGGEWLETRIFSSG
                     PRTNPWFQESGPRIVSDGELLALDTDMIGTYGMCCDISRTWLCGDVEPTAEQIELYRI
                     SYEHIMTNMELLGPGVGFAEITQKAHRLPERFVPNRYGVVMHGVGLCDEYPAIRYRED
                     YDSYGYDGVFEPGMTICVEVYLGAQGGKEGVKLEDQVLITETGYENLTRYPFEEKLLG
                     REF"
BASE COUNT          483 a          851 c          887 g          477 t
ORIGIN      
        1 ctgcgcaacc gctttcggcc gctcgaggcg ctcgacgagg agcagctcga attcattcac
       61 caggtttcgc tgcgcatcgt cgaggagcag ggcatagagg tgatcggcga tctcgcgctg
      121 gacctgtttc gcaacgccgg cgcggaagtc gacgctgacg gcgtcgtgcg catcgaccgc
      181 gcgttactgc tcgaaaccgt cggccgcgcg ccagcggatt tcgaagtgag gacgcgtaac
      241 ccggaacagg cgctggccgt cggcggcgac gtgatcaatt tcggcctggt ctcgggcacg
      301 cccaacgtgc acgacaacat caacggccgg cgcgccggca acttcgagga ttaccgcaag
      361 ctgatcagct tcgggcagta cttcaacgtg ctgacctttt tcggtaacca ggcgaccgcg
      421 ccgaccgatc tgccggccaa ttcgcgccac ctggatacca cttacatcaa cctgacattg
      481 tcggacaagg cctttttcgc caccggtatc ggcggcgggc gggcgcgcga cgcgatcgag
      541 atgagcgcga tcgcgcgcgg cctgacgatg gaggaaatga agactcaccc gtcggtgatg
      601 acgaatatca atatcaattc gccgcgcaag ctcgacgact cgatggcgta cggcgcgatg
      661 cagatggcgc tgttcgggca gccggtaacg gtgacgccgt tcacgctgat gggcgcgatg
      721 accccggcga ccatggccgg cgcgctggcg cagcaaaacg ccgaggcgct ggtcggcatc
      781 gcgctgctgc aactgacgcg gccgggtacg ccctgcgtct acggtgggtt tacgtccaac
      841 gtcgatatgc gctccggttc gccggccttc ggcacgccgg aaaactcgct cgccaatatc
      901 gccggcggcc agctggcgcg caagtacggc ctgccatatc gcagcagcgc ctgcaacgca
      961 tcgaacgcgg tcgacgcgca ggccacttac gaaaccctga tggcgctgtg gagctcggtg
     1021 atgggtcacg gcaacctgat ctatcacacc gccggctggc tcgagggcgg gctggtggcg
     1081 tcgttcgaga agctgatcat cgactgcgaa atgctgcagc acctgagcaa gatgctcgag
     1141 ccgctgaaga tcgatctcga cgaggtaggt ctcgaagcga tgcgagaagt cggccccggc
     1201 ggccatttct tcggttgcgc gcataccatg gagcgctacc ggaacgcgtt ctacgaaccg
     1261 ttcgtcagcg actggcagaa cagcgaaaac tgggtcgccg ccggcagcaa ggacgcgacc
     1321 gcgcgcgcca ccgagctgtg gccgaagatt ctcgaggagt ttacgccgcc gccggtcgat
     1381 gccgcggtgt gcgaggcgct cgaaacttac gtcgccaggc gcaaggaaga actcggcggc
     1441 gaggagccgg ggctcgaacc gaccgattga cgccgggccg gccgcgccgc cgctgctaga
     1501 gaggggtgct cagaattcgc ggccgagcag tttttcttcg aacgggtagc gggtcaggtt
     1561 ttcgtaaccg gtttcggtga tcagcacctg gtcttcgagc ttgacgcctt ccttgccgcc
     1621 ttgcgcgccg aggtagactt cgacgcagat cgtcatgccg ggctcgaaca cgccgtcgta
     1681 gccgtaactg tcgtaatcct cgcggtagcg gatcgccggg tactcgtcgc acaggccgac
     1741 gccatgcatg acgacgccgt agcgattcgg cacgaagcgt tccggcaggc ggtgcgcttt
     1801 ctgcgtgatc tcggcgaagc cgaccccggg gccgagcaat tccatgttgg tcatgatgtg
     1861 ctcataggaa atcctgtaga gctctatttg ctcggcggtc ggctcgacgt caccgcacag
     1921 ccaggtgcgc gagatatcgc aacacatgcc gtaggtgccg atcatgtcgg tatcgagcgc
     1981 gagcagttcg ccgtcgctga cgatgcgcgg gccggattcc tggaaccagg ggttggtgcg
     2041 cgggccggac gagaagatgc gcgtttccag ccactcgccg ccgcgcttga cgttgccggc
     2101 gtgcagcacc gaccagatct cgttttcgct cttgccggcc gcgaccgcgt cctgcatctc
     2161 gtacatcgcg gcttcgcagg cggcgatcgc gcagcgcatc gcgcggattt catcgtcgca
     2221 cttgatgacc cgtgcgatct cggtgaccgc ctgtccttcg aaaacctcga gcccgagctt
     2281 ctccagcgcg cgcagcccgg cgatctcgat tttgtcgacc gccagccggc ggttggcgcc
     2341 ggcatgggtg cgcatcaaat cgtcgatctg cgccgcgaac ttcgccgcgt gctcgtcggt
     2401 gcgagtaccc gactcgaagt aaaaaaacga cgcgccgccg cgcacttcgc ggaccagcgg
     2461 caggtgggtc gacaggtggt caccgccgtg aaagtcgaac agcacgatat agccgtccgc
     2521 cgacacgaag caggcccttg ccgggttgtg cgtgatccac agctgcatgt tggtggaatc
     2581 ggtggcgtag cgtatgttga gcgggtcgta gagcagcagg ccgccgtagt cgttgcgctg
     2641 cagctcctgc accagccgct gcaggcgaaa ctggcgaatc gtatcgagct gcggaatc
//
LOCUS       DATCZV010000321         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_343411, whole genome shotgun sequence.
ACCESSION   DATCZV010000321 DATCZV010000000
VERSION     DATCZV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343411"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..623)
                     /locus_tag="VIW27_07190"
     CDS             complement(132..623)
                     /locus_tag="VIW27_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY5739495.1"
                     /translation="MTEVNSNRASNRVMRWWSTCGKLPAGRYLFSVALGLFIPYTRTI
                     GARVVELQPGYARIELADRRRVRNHLNSIHAIALANIGELTTGLAVFSSTSEEMRGIL
                     TSIRVDYAKKARGKLVSHAEYRLPETITDDVPCEVEALIRDGTGDTVAAVRATWLIGY
                     RRS"
     gene            677..1186
                     /locus_tag="VIW27_07195"
     CDS             677..1186
                     /locus_tag="VIW27_07195"
                     /inference="COORDINATES: protein motif:HMM:NF018946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739496.1"
                     /translation="MDINDRAKLVAAYEHMVENVGNALHEAEEAVAPTVDEMVHNAQQ
                     LARDLFALSQEDAETLGNALRRDLHKADEVLNRQRKELKDWWSFDVALAEDKLAEMVA
                     QAADKTWLDFRAFTSEDHQASVYRSGEICSAGTFSCTHCSAQFAITATAQLQPCHDCG
                     NGEFFRVVA"
     gene            complement(1205..>2362)
                     /locus_tag="VIW27_07200"
     CDS             complement(1205..>2362)
                     /locus_tag="VIW27_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5739497.1"
                     /translation="QTDVADHVLKMLFGAMDELRLGNPWHLSTDVGPVIDAAARDKIV
                     EHCRRMTEQGRVLKTLRVPADGLFVAPTVIELDGIEQLEEEIFGPVLHVARFAAADID
                     AVVDAVNAQGYGLTFGMHTRVDSRVERVVKRIHVGNIYINRNQIGAVVGTQPFGGEGL
                     SGTGPKAGGPWYLPRFTAAPMQLAEPAPSTAVTRLAVEALVAKARHLRASRQPVDEDL
                     LRRIARLQGLDWRVESILGMETMPGPTGETNQLSAHPRGVVLCLGPGREALLAQALTA
                     LAQQNRVVMIDADGAELAALLAAEGLPVTGADGRIDADYLRELEGMDAVMSQASTEIL
                     RDYRIALADRQGRLLPLICESGAVSRLVVERHLCIDTTAAGGNASLIASVS"
BASE COUNT          439 a          778 c          749 g          396 t
ORIGIN      
        1 gatgccaccg gccgccgaca ccgcggcggt cagctgcgga ttgctgcacg gatacatcgc
       61 gccgccgatg accggataac gaatgcccag ttgccggcaa aatgcttgcg cggcctgtgg
      121 aagttcgcga ctcacgagcg ccggtatccg atcagccagg tcgctcgcac ggcggcaacc
      181 gtatcgcccg taccgtcgcg gatcagcgcc tcgacctcgc agggaacatc gtcggtaatc
      241 gtttcgggca gacggtactc ggcatgcgac accagcttgc cgcgcgcctt cttcgcgtaa
      301 tcgacacgaa tactggtcaa aatgccgcgc atctcctcgc tggtcgacga aaaaaccgcc
      361 aggccggtgg tcagttcgcc gatattcgcc agcgcgatgg catgaatcga attcagatga
      421 ttgcgaaccc gccgacgatc ggcgagttcg atgcgcgcat accccggttg cagctcgacg
      481 acgcgcgcgc cgatggtgcg ggtgtagggt atgaacagcc ctagcgcaac gctgaacagg
      541 tagcgcccgg cgggcagctt gccgcaggtc gaccaccagc gcatgacccg gttcgatgcg
      601 cgattggagt tgacttcggt catcaaaaaa ggatgtcggc ttgagtgcgc cgatattatg
      661 cgttattaat gcggtaatgg atatcaatga tagagcaaag ctggtagccg cctacgagca
      721 catggtcgag aacgtcggca atgcgctgca cgaggcagag gaagcggtcg cgccgaccgt
      781 cgatgaaatg gtgcacaacg cccagcaact ggcgcgtgac ctgttcgcgc tcagccagga
      841 agacgccgag acgctcggca atgcgctcag gcgcgacctg cacaaggccg acgaggtcct
      901 gaaccggcaa cgcaaggagt tgaaggactg gtggagtttc gacgtcgcgc tcgccgaaga
      961 caagctcgcc gagatggtgg cgcaagccgc cgacaagacc tggctggatt ttcgcgcctt
     1021 caccagcgag gatcatcagg ccagtgtcta tcgcagtgga gaaatctgca gcgcgggtac
     1081 cttcagctgt acgcattgca gcgctcaatt cgcgattacc gccaccgcgc aactgcaacc
     1141 ctgccacgac tgcggcaacg gcgagttttt ccgcgtcgtc gcctaggcgg ccgcggcgtc
     1201 ccgactaact gacgctggcg atcaggctgg cattaccgcc ggcggcagtg gtgtcaatgc
     1261 acaggtggcg ttcgacgacc agccgcgaga ccgcgccgct ctcgcagatc agcggcaaca
     1321 gcctgccctg ccggtcggcc agcgcgattc gatagtcgcg caggatttcc gtcgaagcct
     1381 gcgacatcac cgcatccatg ccctccagct cccgcagata atcggcgtcg atgcggccat
     1441 cggcaccggt taccggcaag ccctcggcgg ccagcagcgc ggccagttcg gcgccgtcgg
     1501 cgtcgatcat caccacacgg ttttgctgcg ccagcgccgt cagcgcctgt gccagcagcg
     1561 cctcgcgccc cggtccgaga catagcacca cgccgcgcgg gtgcgccgat agctggttgg
     1621 tttccccggt cggtcccggc atcgtctcca taccgagaat cgattccacc cgccaatcga
     1681 ggccctgcag ccgggcgatg cggcgcagca gatcctcgtc cacgggttgc ctggaggcgc
     1741 gcaggtgacg ggccttggcc accagcgctt cgacggctag ccgcgtcact gctgtcgacg
     1801 gtgcgggctc ggccagctgc atcggcgcgg cggtaaagcg cggcaggtac cagggtccgc
     1861 cggccttggg cccggttccc gacaagccct cgccgccgaa cggctgggta cccaccaccg
     1921 cgccgatctg gttgcgattg atatagatgt taccgacgtg tatgcgcttg acgacccgct
     1981 cgacgcgcga atcgacgcgc gtgtgcatgc cgaaggtcag gccgtagccc tgagcgttga
     2041 ccgcatcgac gacagcatcg atatcggcgg ccgcgaagcg cgccacgtgc agtaccgggc
     2101 cgaagatttc ctcctcgagt tgctcgatgc cgtcgagttc gatcacggtc ggcgcgacga
     2161 acaggccatc cgcgggaact cgcagggtct tcagcacccg cccctgctcg gtcatccgcc
     2221 ggcaatgctc gacaatcttg tcgcgcgcgg cagcgtcgat aaccggcccg acgtcggtcg
     2281 acaggtgcca gggattgccg agcctgagct cgtccatcgc accgaacagc attttcagta
     2341 cgtggtcggc gacgtcggtc tg
//
LOCUS       DATCZV010000322        12739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_713893, whole genome shotgun sequence.
ACCESSION   DATCZV010000322 DATCZV010000000
VERSION     DATCZV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713893"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VIW27_07205"
     CDS             complement(<1..804)
                     /locus_tag="VIW27_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY5739498.1"
                     /translation="MENEIFQLQYALDTFYFLVCGALVMWMAAGFAMLEAGLVRSKNT
                     TEILTKNISLYAVACTMYLVVGYDIMYGGGIFLSGIVGDGVTGAAEPATYAPSADFYF
                     QVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTGFIYPMEGSWTWGGASVFGMFTLGD
                     NGFSDFAGSGIVHMAGAAAALAGVILLGARKGKYGKDGQVFAIPGANLPLATLGTFIL
                     WLGWFGFNGGSVLATASVESANAVAVVFMNTNAAAAGGLIAAMIVARIMF"
     gene            complement(814..1152)
                     /gene="glnK"
                     /locus_tag="VIW27_07210"
     CDS             complement(814..1152)
                     /gene="glnK"
                     /locus_tag="VIW27_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5739499.1"
                     /translation="MKIITAIIKPFKLDDVRQALGEIGVQGLTVSEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEIAIDDDLLDQAIEAITNSANTGKIGDGKIFITPLEQVIRI
                     RTGESGSAAL"
     gene            1297..1542
                     /locus_tag="VIW27_07215"
     CDS             1297..1542
                     /locus_tag="VIW27_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY5739500.1"
                     /translation="MIDNQTINRLSEQIQALLPPGLQQVKADFDARLKVLLQQQLARY
                     EMVSREEFDIQTRVLARTREKLETLEARLKELEQADE"
     gene            1651..3156
                     /locus_tag="VIW27_07220"
     CDS             1651..3156
                     /locus_tag="VIW27_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY5739501.1"
                     /translation="MSLTQLITRAQLGIDAPEVRVEVHISNGLPAFTLVGLAETAVRE
                     ARDRVRSAIINSGFEFPDRRITVNLAPADLPKEGGRYDLPIALGILAASEQVEITPLA
                     RSACFGELALGGECRGVRGLLPALIACKQGGNAAVVPRANHAEAALVRGLPVSLGDSL
                     ARICASLNGGDPLEQITARPATHAAHYRLDLADVIGQQQARRALEIAAAGGHHMLMMG
                     PPGSGKSMLAQRLITILPAMDEDEAMSLASLRSICGHPVNPETWLQRPFQSPHHTVSA
                     AALAGGGSHPRPGEISLAHNGLLFLDELTEFRRDVIEVLREPLETGQIHISRAAGKVT
                     YPARFQLIAAMNPCPGGCDTISACNCSPQQLQRYRNRLSAPLLDRIDIQIELGKLRQS
                     ELLEAEKAPRESSASIRRRIECARARQLSRQACVNAQLDQRKISQVCRLQGGLEQMLA
                     EAIDRLGLSARSYHKLLKLARTIADLADSERIGHAHLAEAIAYRRRENLRA"
     gene            complement(3300..3773)
                     /locus_tag="VIW27_07225"
     CDS             complement(3300..3773)
                     /locus_tag="VIW27_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5739502.1"
                     /translation="MSESQSETFSTHNIAVFGGLLLIAVFIGEVLIGPQGDQQPVAVS
                     ADTTEDIAMRLKPVVTLDEVRDNMTVAAGAGTDADKPPAELYQSACLACHSTGAAGAP
                     RIGDATAWAARLVKGVDALVSSAVNGIGAMPPRGGSQISDEQIRAVVEYILDESR"
     gene            3928..4004
                     /locus_tag="VIW27_07230"
     tRNA            3928..4004
                     /locus_tag="VIW27_07230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3962..3964,aa:Arg,seq:ccg)
     gene            4333..5772
                     /locus_tag="VIW27_07235"
     CDS             4333..5772
                     /locus_tag="VIW27_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY5739503.1"
                     /translation="MTVTRSDTYKYDQVSRYIDELIEKGDLKPGDKAPSLRKLSKQLG
                     VSIATVTQAYVNLEDQGVLSAKPQSGFYVNALVNQIHDIDKSAATPRRARRVRFGELF
                     EEVFRNANNPRMVRFGTSNPAMDFLPVKSLTRATRSIISRYPQKSMDYLFPPGHRKLR
                     EQIAQQYAQNYTRVSADDIIITSGATEALSISLRTVAKRGDIIAVESPTYFAVLRMIE
                     QMGMLAVEIDTDPVTGLSLDALEEAFDTMDIKAVLASPNTSNPLGSQMPEERKRELVN
                     LLAERDIPLIEDDVYGSVYFGDKQPRPAKYYDLNRIVLSCSSFSKTLAPGHRIGWVIA
                     GRYHKKFLQYKQAWSSATSSINQLALAEFLSSGQYDRHLVRLRMAMREQVERGRFMIA
                     KCFPEGTRISHPHGGNVLWVEMPKDCDSIDIFNRALENNIAVVPGILFSATRRFRNHL
                     RINCGSPWTAETEAALKTLGQIVCNCKSG"
     gene            complement(5769..7619)
                     /locus_tag="VIW27_07240"
     CDS             complement(5769..7619)
                     /locus_tag="VIW27_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5739504.1"
                     /translation="MLAFSGLALRRASTLLFEDASFKVFAGQKLGITGANGCGKSSLF
                     ALISGELGADQGEFSMPDDWAVSAVEQETRASDRVALEYVIDGDAELRQTQSRLERAE
                     RDHDSTQIAYCHERLQEIDGYQASSRAARLLAGLGFDDAEIRRPLNSFSGGWIMRLNL
                     ARALMCRSDLLLLDEPTNHLDLDAVIWLEQWLQRYQGCLLLISHDRDFLDAVVDSIAH
                     IENQRISLYKGNYSAFELQRAERLSQQQAAYEKQQQSIAHMQRYIDRFRAKATKAKQA
                     QSRIKALERMQLIAPAHVDSPFHFEFREPEALPSSLLRLQQVSTGYGERVVLDGLEFS
                     LIPGERIGLLGLNGAGKSTFIKLLADELQPFTGEITRARHLRIGYFAQHQVEQLDLDA
                     SPLLILQRLAAELSERQVRSFLGGFNFRADKVLQPTGSFSGGEKARLVLALLLWQRPN
                     LLLLDEPTNHLDLEMRLALNQALQGFRGSVILVSHDRHLLRSVCDDLWLIDGGRLRPF
                     EDDLDAYPAWLARRKAGGAGGGEPTGGRRKREFKAQRNRISKLEREIEQLTEVREGLL
                     ARLGEDSIYLEEHRDELRTALAAQAENQQRLEHAEEDWLRLSEALEAQQD"
     gene            7732..8445
                     /locus_tag="VIW27_07245"
     CDS             7732..8445
                     /locus_tag="VIW27_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3581 family protein"
                     /protein_id="HEY5739505.1"
                     /translation="MNLEEYYNLDGPRLSFTRQQASDFAKKIAGDFNSIHDTDSKRFC
                     VPGDLLFAVILRHYGLRQVMGFSFSGMVGDDVSLILPEVNAREISVYDENDRKYLDVS
                     SNGEHTQSVALIESLTRSYVEFSGLTFPQVLVPLMKDNDVMINTERPLVIYDHMRISL
                     DRLDIDSVDLELTDPVFRLYGKRGDVALNFDLLSNGKSIGRGQKKIVLSGLRQYEQAR
                     IDELIEAYEGRRRRFADGD"
     gene            complement(8442..9143)
                     /locus_tag="VIW27_07250"
     CDS             complement(8442..9143)
                     /locus_tag="VIW27_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739506.1"
                     /translation="MPGKFSSITDYRGSRPCSCRGIAGGLLLLLAFHSGIAGAEWTGV
                     TLDLANTDSDWKFRGQVREARNSELSFSIEERAASGLAIGVQIGYFDMRLIAPDETET
                     LKFDGQYFGIYLRHELAIGERLSLHSGLGLKYASGTESGDAEDQAEIDWIETDVEIGI
                     GIRAGNLRIMPFAAWNDVDGDISDENGTSVFNLAQSDTQGVRFDLYVEQTAFVRLELV
                     TGSRSGGTLVFARRY"
     gene            complement(9177..9938)
                     /locus_tag="VIW27_07255"
     CDS             complement(9177..9938)
                     /locus_tag="VIW27_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5739507.1"
                     /translation="MVNPFYKVWLVIRATLFWIFFLPMLLLLATLLSLLFFAPLGVRV
                     GVVHIWIAYSLFCLRLFCGLTHEVEGLENIPDDGFIVMSRHSSTWETISIQRYFRPMI
                     WVVKRELTWIPFFGWALKAMDAIALNRGTGRKAINQLVNESRPFMDRGRILMLFPEGT
                     RVPPGGYRPFKLGGAIVSQRTGYAILPIAHNAGEYWPRHSWIKWPGKIRMVIGKPIDP
                     AGKTPEQIVREVEDWVLAECERISDKEQLRRLGVL"
     gene            complement(9938..10480)
                     /gene="gmhB"
                     /locus_tag="VIW27_07260"
     CDS             complement(9938..10480)
                     /gene="gmhB"
                     /locus_tag="VIW27_07260"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY5739508.1"
                     /translation="MVILDRDGVINHVSEDDVTTVDGWDPIAGSIEAIGRLKKAGFLV
                     TIASNHSGISRGLYSEDELTSMHDKMQRMLAARGAGVDGIFYCPHGPEANCICRKPKP
                     GLLFRIAERFEVDLSQTPLVGDNIDDIRAARLARAQPVLVRTGYGEYVMQNYPEALDV
                     PVYDDLSHFVRDTLRGRSAE"
     gene            complement(10519..12588)
                     /gene="glyS"
                     /locus_tag="VIW27_07265"
     CDS             complement(10519..12588)
                     /gene="glyS"
                     /locus_tag="VIW27_07265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY5739509.1"
                     /translation="MQTSDCLIEVGTEELPPRALQTLARDFAALLERTLTEQDLAPAR
                     SEIFATPRRLAMLLRDTPLQQAEQTIEKRGPALDAAFDADGQPTRAALGFAASCGVEV
                     DSLERRQTDRGSWLYYRSSAAGKRLQEILPQALERALAELPIPKRMRWGDRSDEFVRP
                     VRWLVAMIGDEIVDAEAFGMKAGRETRGHRFHAPQVLEIPTAADYEQILATQGWVIAS
                     FETRRARIRQLVEDAAAELGGEARIDAALLDEVTALVEFPVAICGRFDAAFLQLPAEV
                     LVMTMQENQKYFALFDDDGQLLPHFIAIANIDSRTPELVAHGNERVIRPRFADAEFFF
                     NQDRKRGLDAMRMRLDAVVFQEKLGSLGERTARIIRLAKYIASHTGADQDLVRRAADL
                     CKADLMSDMVGEFPKLQGVMGKYYARLQQEAAPVSEAIEQHYWPRHAGDRLPEAPVAQ
                     AVALAERLDSLVGIFAIGEKPGGDKDPFALRRAALGVLRIIVEQRLGLDLRDLCQAAA
                     DGYPKNLDAAAAIDEVIEFVFDRLRGYYREQGIGVDIVDAVACGRPDSLYDCDLRIRA
                     LEEFQSHEAASALAAANKRIGNILKKQAAAPGEIDPALLQEDAERELYEQLGQRRDTA
                     AARFEQGDYLSGLEQLAELRPAVDRFFDQVMVMVDEPALRDNRLALLDQLLQSFRRVA
                     DFSRIRQ"
     gene            complement(12578..>12739)
                     /locus_tag="VIW27_07270"
     CDS             complement(12578..>12739)
                     /locus_tag="VIW27_07270"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY5739510.1"
                     /translation="FNLLDARHAISVTERQRFILRVRALSRAVAESYYASREALGFPL
                     IKTEQAHAD"
BASE COUNT         2504 a         4061 c         3865 g         2309 t
ORIGIN      
        1 gaacatgatg cgcgccacga tcatcgccgc gatcagaccg ccggcggccg cggcgttggt
       61 attcatgaac accacggcga cggcgttagc cgactcgacc gacgcggtgg caagcaccga
      121 gccgccgttg aagccgaacc agcccagcca aaggatgaag gtgccgaggg tggccaacgg
      181 caggttggcg ccgggaatcg cgaatacctg gccgtccttg ccgtacttgc ccttgcgagc
      241 gccgagcagg atgactccgg cgagcgcggc ggccgcaccg gccatgtgca cgatacccga
      301 accggcgaaa tcggagaagc cgttgtcacc gagcgtgaac atgccgaaca ccgatgctcc
      361 accccaggtc cagctaccct ccatcggata gatgaaaccg gtcatgacga cggcgaaggc
      421 gagaaacgcc cacagcttca tgcgctcggc aacggcgccc gaaacgatcg acatcgcggt
      481 agcgacgaac accacctgga agtagaaatc cgccgacggc gcataggtgg ccggctccgc
      541 ggcaccggtg acgccgtcac cgactatccc cgacaggaat atgccgccgc cgtacatgat
      601 gtcgtagccg acaaccaggt acatggtaca ggcgaccgcg tagagtgaaa tattcttggt
      661 cagaatctcg gtggtgttct tggagcgcac caggcccgcc tcgagcatcg cgaagcccgc
      721 agccatccac atgacaaggg cgccgcaaac gaggaagtaa aaggtgtcta gagcgtactg
      781 taactgaaaa atctcgtttt ccatggcgtc ctcctatagt gcagccgaac cggattcacc
      841 cgtacgaata cggatcacct gttcgagggg ggtgatgaaa attttgccgt caccgatctt
      901 gccggtattg gcgctgttgg ttatcgcctc gatcgcctgg tcgagaagat cgtcgtcgat
      961 cgcaatctcg agtttgacct tgggtaaaaa gtcgacgacg tattcggcac cccgatacaa
     1021 ctcggtgtgc cccttctggc gtccgaagcc cttgacttcc gaaacggtca gaccctgcac
     1081 cccaatttcg ccgagggcct ggcgaacgtc atcgagcttg aacggcttga ttatcgcggt
     1141 aatgattttc atgactaact ctccgaatag aaccggcctc gcattttggg gactctggga
     1201 aatcaagcaa ataaacggta atatcgaagc caatggcagc ctttacgcac aactattgtg
     1261 caatatggct gatgtacgca ctattttggg gcttttatga tcgacaatca gactatcaat
     1321 cgcctgtccg agcaaatcca ggcgctgttg ccgcccggcc tgcaacaggt caaggcggat
     1381 ttcgacgctc gcctgaaagt cctgctgcaa cagcaattgg cccgctacga aatggttagc
     1441 cgcgaggaat tcgacattca gacccgggtg ctggcccgta ctcgcgaaaa gctcgaaacc
     1501 ctcgaggcgc gactgaagga actcgagcaa gccgacgagt aacgcattcg gcgcaccgag
     1561 ccacgcctcg aaatcggccc gaatcgtgtt agactggcag tcgccgcgcc ggacgcgcgg
     1621 cccgttaacg gcgacgcaaa ggattctgcc atgtcactca cccagctcat cacccgtgcg
     1681 caactcggta tcgacgctcc cgaggttcgc gtcgaagtgc atatttccaa cggcttgccc
     1741 gccttcaccc tggtcgggct tgcggaaacc gcggtgcgcg aggcgcgcga ccgggtgcgc
     1801 tcggcgatca tcaactcggg attcgagttt cccgaccgac gcatcaccgt caacctggcg
     1861 ccggcggatc tgcccaagga aggcggccgc tacgatctgc cgatcgcact cggcatactc
     1921 gccgcctccg agcaggtgga gatcacgccg cttgcgcgca gtgcctgctt cggcgaactg
     1981 gcgctcggcg gcgaatgccg cggtgttcgc ggtttgctgc cggcgttgat tgcctgcaaa
     2041 caaggcggca atgcggccgt ggtgccgcga gccaatcacg ccgaggccgc gctggtgcgt
     2101 ggtctgccgg tcagtctcgg cgattcgctg gcccgcatct gcgccagcct caacggtggc
     2161 gatccactcg aacaaataac agcgcggcca gccacgcatg cggcgcacta ccgactcgat
     2221 ctcgccgacg tcatcggcca gcaacaggcc cgccgcgcgc tcgagatcgc cgctgccggc
     2281 gggcaccaca tgttgatgat ggggccgccg ggttcgggca agagcatgct cgcgcagcgg
     2341 ctgatcacca tcctgccagc aatggacgaa gacgaagcca tgagtctcgc cagtctgcgc
     2401 tcgatttgcg ggcatccggt taaccccgaa acctggctgc agcgcccgtt tcaatccccg
     2461 catcacacgg tatcggcggc ggcgctggcc ggcggtggca gtcatccgcg tcctggcgag
     2521 atttcactgg cccacaacgg gctgttgttt ctcgatgagc tgaccgagtt tcgacgcgac
     2581 gtgatcgaag tgttgcgcga accgctggaa accgggcaaa ttcacatctc ccgcgctgcg
     2641 ggcaaggtga cttacccggc ccgctttcaa ttgatcgcgg caatgaaccc ctgcccgggc
     2701 ggctgtgaca cgatcagcgc ctgcaattgc tcaccgcagc agttacagcg ctaccgcaac
     2761 cggctgtcag cgccgctgct cgaccgcatc gacattcaga tcgaactcgg caagttgcgg
     2821 caaagcgaac tgctcgaggc cgaaaaagcg ccccgcgaga gcagtgccag tatccgccgg
     2881 cgcatcgagt gcgcccgcgc ccgccagctg tcgcggcagg cttgtgtcaa cgcgcagctc
     2941 gatcagcgaa aaatttccca ggtctgccgc ctgcagggcg gactcgagca aatgctggcg
     3001 gaggcgatcg acaggctggg cctgagcgca cgcagttacc acaagctgct taaactggcg
     3061 cggaccatcg ccgatctcgc ggacagcgag cgcatcggcc acgcgcatct cgccgaggcg
     3121 atcgcctatc gacgacgcga gaatctgcgc gcatgagatg gcatcgcgac agacttcgcg
     3181 gccggggccg gactactgac gggaatcggg gtcgggcggt taacgccagc cgcggcgcgg
     3241 cgaggatgcg ctcgaactgc gcaggttgtt gccggccggg cgggtcgccg tgacggccgt
     3301 tagcgggact cgtcgaggat gtactcgacg accgcgcgga tctgctcgtc gctgatctgc
     3361 gatccgccgc gcggcggcat tgcgccgatg ccgttgacgg ccgagctgac caacgcatcc
     3421 acacccttta cgagacgcgc ggcccacgcg gtggcatcgc cgatcctcgg tgcgccggcg
     3481 gcgccggtgc tgtggcaggc gaggcacgcg ctctggtaaa gttcggccgg cggcttgtcg
     3541 gcatcggtac cggcacccgc ggcgacggtc atgttgtcgc gaacctcgtc gagggtgacc
     3601 accggcttca ggcgcatcgc gatatcctcg gtggtgtccg ccgacacggc caccggctgc
     3661 tgatcgccct gcggcccgat gaggacttct ccgataaaga cggcgatcaa caacaggccg
     3721 ccgaacacgg cgatattgtg cgtcgaaaaa gtttcggatt ggctttcgct cacgaggatc
     3781 tccaggcagt cggggcagag taaaaacgct gcgaattatc gtcgaaacgc cgcgaattcg
     3841 caaaaattat ttggcttcgg tgcctaaagc ggtccacgat ggtttacaat caccgccgtg
     3901 ctcacggggc actgcagcaa acaccaggcg ctcgtagctc agttggatag agtgttggcc
     3961 tccgaagcca aaggtcgtgg gttcgactcc cgccgagcgc gccattttcc caaagcccgc
     4021 ccatgcgggc tttttcattc gtgccatcgc gccgacagta ccggccgcgg gttgccgacc
     4081 ttacgctgcg cggcgtatcc gtcgcaggcg aatttcatgc actcgcacac actaggccgg
     4141 ggcaaaaaaa aacccgccgg tgttagcgac gggtcaacta caagtcaaaa cctgaggagg
     4201 aggagggggg tttcaacttt ttgccggagc agcactgctg ccgcaacggc aatgatgcaa
     4261 taataaagac gaaactgtta caataacagt tacaattttt taaaaatcga tacattacag
     4321 ttgtgagtaa ccatgacagt tacgcgtagc gacacttaca aatacgacca ggtttcgcgt
     4381 tatatcgacg aattgatcga aaaaggcgat ctcaagcccg gcgacaaggc gccgtcgcta
     4441 cgcaaactga gcaagcagct cggcgtcagc atcgccaccg taacccaggc ctacgtcaac
     4501 ctggaagacc agggagtgct gtcggcgaag ccgcagtcgg gattctatgt caatgcgctg
     4561 gtgaatcaaa tacacgatat cgacaaatcc gcggcaaccc cgcggcgcgc gcggcgtgta
     4621 cgcttcggcg agttgttcga ggaagttttt cgcaacgcca acaacccgcg catggtgcgc
     4681 ttcggcacgt ccaacccggc gatggacttc ctgccggtta agtcgctgac gcgggcgacc
     4741 cgcagcatca tcagccgcta cccgcaaaaa agcatggatt acctgtttcc gcccgggcac
     4801 cgcaaactgc gcgagcagat cgcccagcag tacgcgcaaa actacacccg tgtcagcgcc
     4861 gacgatatca tcatcacctc gggcgccacc gaggcgctgt cgatcagcct gcgcaccgtg
     4921 gccaagcgtg gtgacatcat cgccgtcgag tcgccgacct acttcgcggt gctgcgcatg
     4981 atcgagcaaa tgggtatgct ggcggtagaa atcgataccg atccggtgac cggcctgagc
     5041 ctcgacgcgc tcgaggaagc ctttgacacc atggatatca aagcggtgct ggcgtcgccc
     5101 aataccagca acccactcgg cagccagatg cccgaagagc gcaagcgcga actcgtcaac
     5161 ctgctcgccg agcgcgatat cccgctaatc gaggacgatg tctacggcag cgtctatttc
     5221 ggcgacaagc agccgcgccc cgccaagtac tacgacctca acaggatcgt gctgagctgc
     5281 tcgtcgtttt ccaagaccct ggcgccaggc caccgcatcg gctgggtgat cgccggccgc
     5341 taccacaaga aattcctgca gtacaagcag gcctggtcgt ctgccacctc gtcgatcaat
     5401 cagctggcgc tggccgaatt cctcagcagc ggccagtacg accgtcacct ggtgcgcctg
     5461 cgcatggcga tgcgcgagca ggtcgagcgc ggtcgtttca tgatcgccaa gtgctttccc
     5521 gagggtacgc gcatttcaca cccgcacggc ggcaacgtgt tatgggtcga aatgccgaag
     5581 gattgcgaca gcatcgatat cttcaatcgc gcgctggaaa acaacatcgc cgtggtaccc
     5641 ggcatcctgt tctcggccac ccgtcgcttt cgcaaccacc tgcgtatcaa ctgcggctcg
     5701 ccgtggacag cggaaaccga ggccgcactg aagacgctgg gtcagatcgt ctgcaactgc
     5761 aagagcggct agtcctgctg cgcttcgagt gcctcgctga ggcgcagcca gtcttcctcc
     5821 gcgtgctcca gccgctgctg gttctcggcc tgcgcagcca gcgcggtccg cagttcgtcg
     5881 cggtgttcct cgaggtatat cgaatcttcg ccgagtcgtg cgagcaggcc ctcgcggact
     5941 tcggtgagtt gctcgatttc gcgttccagc ttgctgatcc tgttacgctg cgccttgaac
     6001 tcgcgcttgc ggcgcccgcc ggtcggctcg ccgccgcccg caccgcccgc cttgcgccgc
     6061 gccaaccatg ccgggtaagc gtcgagatcg tcctcgaacg gccttagcct gccgccatcg
     6121 atcagccaca ggtcgtcgca aacgctgcgc agcaaatgcc gatcgtgcga taccaggatg
     6181 acgctgccgc ggaaaccctg cagtgcctgg ttgagcgcca gccgcatctc gaggtcgaga
     6241 tggttggtcg gctcgtcgag cagcagcagg ttgggacgct gccagagcag cagcgccagc
     6301 accagacgcg ccttctcgcc gcccgagaaa ctgccagtcg gttgcagcac cttgtcggcg
     6361 cgaaaattga agccgcccag aaaactgcgc acctggcgct cgctgagttc cgctgccagc
     6421 cgctgcagga tcagtagcgg gctggcatcg agatcgagct gctcgacctg gtgctgcgcg
     6481 aaataaccga tgcgtaagtg tcgcgcgcga gtgatctccc ccgtgaacgg ttgcaattcg
     6541 tccgctagca gcttgatgaa ggtcgacttg ccggcgccgt tcagacccag cagcccgatg
     6601 cgttcgccag gaatcagcga aaactcgagg ccgtcgagca ccacgcgctc gccgtaaccg
     6661 gtcgatacct gctgcaggcg aagcagtgaa ctcggcagcg cctcgggttc gcgaaactcg
     6721 aaatgaaacg gtgaatcgac gtgtgccggg gcgatcaact gcattctttc cagcgccttg
     6781 atgcggcttt gcgcctgctt cgccttggtc gccttggcgc gaaagcgatc gatgtagcgt
     6841 tgcatgtggg cgatgctttg ctgctgtttt tcgtaggccg cctgctgctg cgacaagcgt
     6901 tcggcgcgtt gcagctcgaa agccgagtaa ttccccttgt agaggctgat tcgctggttc
     6961 tcgatatgcg cgatgctgtc gaccacggca tccagaaaat cacgatcgtg agaaatcagt
     7021 aacaggcagc cctgatatct ttgcagccac tgttcaagcc agattaccgc gtcgaggtcg
     7081 agatggttgg tcggctcgtc gagcagcaac aggtcggaac ggcacatcag cgccctggcc
     7141 aggttgaggc gcatgatcca gccgccggaa aaactgttta gcggccggcg gatctcggca
     7201 tcgtcgaagc ccagcccggc gagcaaccgc gccgcgcggc tgctcgcctg atagccatcg
     7261 atttcctgca gccgctcgtg gcagtaggcg atctgcgtcg aatcatgatc gcgctcggcg
     7321 cgctcgagcc tcgactgggt ctgccgtaac tcggcgtcgc cgtcgatgac atactcgagc
     7381 gccacgcgat cgctggcacg ggtttcctgc tcgaccgcgg acaccgccca gtcgtccggc
     7441 atgctgaact cgccctggtc ggcgccgagt tcgccgctga tcaatgcgaa caacgaagat
     7501 ttgccgcagc cgttggcccc ggtaatgccg agcttctggc cggcgaacac tttgaaactg
     7561 gcgtcttcga acagcagcgt cgatgcacgc cgcaacgcga ggccgctaaa ggcgagcatc
     7621 ggcgacaccc gcagccggcg ccctgagggc attatcgatc acttttagta cacccggggg
     7681 aaatggggta agataccatc ccgccccgca gaaagttaag ccgcgtccga aatgaacctg
     7741 gaagaatact ataacctcga tgggcccagg ctttccttta cccgtcagca ggcgagcgat
     7801 ttcgcgaaaa aaatcgccgg tgacttcaac agcattcacg acaccgattc gaagcgtttt
     7861 tgcgtgcccg gggatctact gttcgcggtc atcctcaggc attacgggtt gcgccaggtc
     7921 atggggtttt cgttttccgg catggtcggc gacgacgtct cgctgatcct gccggaagtc
     7981 aacgcccgcg agatctcggt ctacgacgaa aacgacagaa aatacctcga cgtctccagt
     8041 aacggcgaac atacgcaaag tgtggcgctg atcgaatcgc tgacccgcag ctacgtcgag
     8101 ttttcaggcc tcacctttcc gcaggtgctg gtgccgctga tgaaggataa cgacgtgatg
     8161 atcaacaccg agcggccgct ggtgatctac gaccacatgc gcatctcgct ggacaggctg
     8221 gatatcgact cggtcgacct cgagctgacc gacccggttt ttcgactcta cggtaagcgc
     8281 ggcgacgtgg cgctgaactt cgacctgctg agtaacggca aatccatcgg ccgggggcag
     8341 aaaaaaatcg tgcttagcgg cctgcgtcaa tacgagcagg ccaggatcga cgagctgatc
     8401 gaggcttatg aaggccgtcg ccggcgcttt gccgacggcg actaataccg ccgagcgaag
     8461 accagggtgc cgccggagcg gctaccggtc accagttcca ggcgcacgaa cgcggtctgt
     8521 tcgacgtaca ggtcgaaacg gactccctgg gtatccgact gcgcaagatt gaatacgctg
     8581 gtgccgttct cgtcgctgat atcgccgtcg acatcgttcc aggccgcgaa cggcatgatc
     8641 ctcaggttgc cggcgcgaat cccgatgccg atctcgacat cggtctcgat ccagtcgatc
     8701 tcggcctggt cttcggcgtc gccgctctcg gtgccgctgg cgtacttcag gccgagcccg
     8761 ctgtgcagcg acagccgctc accgatcgct aactcgtgcc gcaggtagat gccgaaatac
     8821 tggccgtcga acttgagcgt ctcggtttcg tccggcgcga tcaggcgcat gtcgaaatag
     8881 ccgatttgga cgccaatagc caggccactg gcggcgcgct cctcgatgct gaaactgagt
     8941 tcgctgttgc gggcttcacg tacctggccg cgaaatttcc agtcgctgtc ggtattggcg
     9001 aggtccagcg tgacgccggt ccactcggca ccagcgatac ccgaatgaaa agccagtaac
     9061 agcagcaggc cgccggcgat tccgcggcag ctacaaggcc gtgaaccgcg gtaatcggtg
     9121 atgctggaga atttgccggg catgaggttc caccaaacca gggagttgat catcgattat
     9181 agaacaccga gtcgccgcaa ctgttccttg tcactgatgc gctcgcactc cgcgagaacc
     9241 cagtcctcga cttccctgac gatttgctcc ggggtcttgc cggcggggtc gatcggctta
     9301 ccgatgacca tgcggatttt tcccggccac ttgatccagc tgtgccgtgg ccagtattca
     9361 ccggcattgt gcgcgatcgg cagtatcgca tagccggttc gttgcgaaac gatggcgccg
     9421 ccgagcttga acggccgata gcctcctggc ggcacgcgcg taccttccgg gaacagcatc
     9481 agaatgcgcc cgcggtccat gaacggacga ctctcgttca ccagctgatt gatcgccttg
     9541 cgaccggtac cgcgattcaa cgcaatcgcg tccatcgctt tcagcgccca gccgaagaaa
     9601 ggtatccagg tcaactcgcg cttgaccacc cagatcatcg gccgaaagta acgctgaatg
     9661 gagatcgtct cccaggtcga gctgtgacgg ctcatgacga taaagccgtc gtcgggtatg
     9721 ttttctagac cctcgacctc gtgggtcagt ccgcaaaaca gtcgcaggca aaacagcgag
     9781 tacgcgatcc agatgtggac cacgccgacg cgcacgccaa gcggtgcaaa gaacagcagc
     9841 gacagcaggg tggccagcag caacagcatc ggcagaaaaa aaatccagaa cagcgtcgcg
     9901 cgaatgacca gccagacctt gtagaaggga ttgaccatca ctcagctgac ctgccgcgca
     9961 gcgtatcgcg gacgaagtgg gagagatcgt catacaccgg cacgtcgagc gcttccgggt
    10021 aattctgcat cacgtattcg ccgtaaccgg tgcgcaccag cactggttgc gcgcgcgcca
    10081 gccgggccgc gcgtatatcg tcgatattgt cgccgaccag cggcgtttgc gagagatcga
    10141 cttcgaatcg ctcggcgatg cgaaacagca gccccggctt gggcttgcgg cagatacaat
    10201 tggcctcggg accgtgcggg cagtagaaaa ttccatcgac gccggcgcca cgcgcggcca
    10261 gcatgcgctg catcttgtcg tgcatcgagg tcagctcgtc ctcgctgtac aggccgcgcg
    10321 atatgcccga gtggttgctg gcgatagtca ccaggaaacc ggccttcttg agcctgccga
    10381 tggcttcgat actgccggcg atcgggtccc agccgtcgac cgtggtgaca tcgtcctcgg
    10441 acacgtggtt gatgaccccg tcccgatcga ggataaccat tcttgccacg caatcctccc
    10501 cggctgcgag ttgcggcgtc attgccgaat gcgcgaaaaa tcggcgaccc ggcggaaact
    10561 ttgcaacagt tgatcgagta gcgcaagccg gttgtcgcgc agtgccggct cgtcgaccat
    10621 caccatgacc tggtcgaaaa agcggtcgac cgcaggccgc aactcggcca gctgctcgag
    10681 cccggacagg taatcaccct gctcgaaccg ggccgccgcg gtatcgcgcc gttgcccgag
    10741 ctgctcgtaa agttctcgct cggcgtcttc ctgcaacagg gccggatcga tctcgccggg
    10801 cgccgcggcc tgtttcttga gaatattacc gatgcgcttg ttggcggcgg ccagcgcgct
    10861 cgccgcctcg tgactctgaa attcctcgag cgcgcgaata cgcagatcgc aatcgtagag
    10921 ggaatcgggc cggccgcagg cgaccgcatc gacgatatcg acgccgatgc cctgttcccg
    10981 gtagtagccg cgcagtcgat cgaagacgaa ttcgatcact tcgtcgatgg ccgcggcggc
    11041 atcgagattt tttggataac cgtcggcagc cgcctggcag aggtctctta gatccagacc
    11101 caaccgctgc tcgacgatga tgcgcagtac gccgagcgcc gcgcggcgca gagcgaaggg
    11161 atccttgtcg ccgcccggct tctcgccgat cgcgaaaata ccgaccaggc tgtctaggcg
    11221 ctcggccagt gctactgcct gagcgaccgg ggcttccggc aggcggtcgc cggcatgcct
    11281 gggccagtaa tgctgctcga tcgcctcgct gaccggcgcg gcttcctgtt gcaggcgcgc
    11341 gtagtacttg cccatcacac cctgtaattt cgggaactcg ccgaccatgt cgctcatcag
    11401 gtcggccttg caaagatcgg cggcgcggcg caccaggtcc tggtcggcgc cggtgtggct
    11461 ggcgatatac ttggcgagtc gaatgatacg cgcggtgcgc tcgccgagac tgccgagctt
    11521 ctcctggaac actaccgcgt cgaggcgcat gcgcatcgcg tcgagcccgc gcttgcgatc
    11581 ctggttgaaa aagaattcgg catcggcaaa acgcggccga atcacgcgtt cgttgccatg
    11641 ggcgaccagc tcgggtgtgc ggctgtcgat attggcgatg gcgatgaaat gcggcagcag
    11701 ctggccatcg tcatcgaaca gcgcgaagta cttctggttt tcctgcatcg tcatcaccag
    11761 cacttccgcc ggcaattgca aaaaggcggc atcgaaccgg ccgcagatcg ccaccgggaa
    11821 ctcgaccagc gcggtgacct cgtcgagcag cgcggcgtcg atgcgcgcct cgccgccgag
    11881 ttcggctgcc gcgtcttcga ccagctggcg gatccgcgca cggcgggtct cgaaactcgc
    11941 gattacccag ccctgcgtgg cgaggatctg ttcgtaatcg gcggccgtcg gaatctccag
    12001 cacctgcggc gcgtgaaaac ggtgtccgcg ggtttcgcgg cccgccttca tgccgaaagc
    12061 ctcggcatcg acgatctcgt cgccgatcat cgccaccagc cagcggaccg gacgcacgaa
    12121 ctcgtcgcta cgatcacccc agcgcatgcg cttgggtatc ggcagctcgg cgagcgcgcg
    12181 ctcgagcgcc tgcggcaata tctcctgtaa tcgcttgccg gcggccgacg atcgatagta
    12241 aagccagctg ccgcggtcgg tctggcggcg ctccaggctg tcgacctcga cgccgcagga
    12301 ggccgcgaaa ccgagtgcgg cgcgcgtcgg ctggccgtcg gcatcgaagg cggcgtcgag
    12361 tgccggaccg cgtttctcga tcgtctgctc cgcctgctgc agcggcgtgt cgcgcaacag
    12421 catcgccagc cgccgcggcg tcgcgaaaat ttcgctgcgc gccggcgcca ggtcctgctc
    12481 ggtcagcgtc cgctcgagta acgcggcgaa atcgcgcgcc agcgtctgca gcgcccgcgg
    12541 cggcaattcc tcggtgccta cctcgatcag gcaatcgcta gtctgcatgc gcctgctccg
    12601 tcttgatcag cggaaagccg agcgcctcgc gcgacgcgta gtagctttcg gcgaccgcgc
    12661 gtgacagggc gcgcacgcgc agaatgaatc gctgccgctc ggtaaccgaa atcgcgtggc
    12721 gcgcgtcgag caggttgaa
//
LOCUS       DATCZV010000323         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_343485, whole genome shotgun sequence.
ACCESSION   DATCZV010000323 DATCZV010000000
VERSION     DATCZV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343485"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VIW27_07275"
     CDS             complement(<1..322)
                     /locus_tag="VIW27_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739511.1"
                     /translation="MFATLYALESYARALLSTVITVQALQLLGNARDVSLLFSAVGVT
                     GLLASFSIPVLIRHLSRRWVYTLGALLIFAAAASLAMVSLAGQTLGMLFRVYGTACLN
                     ITLSL"
     gene            complement(505..1068)
                     /gene="comE"
                     /locus_tag="VIW27_07280"
     CDS             complement(505..1068)
                     /gene="comE"
                     /locus_tag="VIW27_07280"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit beta"
                     /protein_id="HEY5739512.1"
                     /translation="MIRSEVLKSLVPVISDQLVVCNIGLPSQELHMLDDQPSNFYMLG
                     TMGLASSIGLGLALAQPQKVIAIDGDGSVLTNFGTLPTIANNVADNFILLIIDNGSYG
                     STGDQPTYAGRKTSLAAVATACGCDNVVECSAEDTADAVRAALDGERMTIIVCKCESG
                     NIKVPVIDKDPVVIRQRFMDEIRKRNG"
     gene            complement(1065..1565)
                     /gene="comD"
                     /locus_tag="VIW27_07285"
     CDS             complement(1065..1565)
                     /gene="comD"
                     /locus_tag="VIW27_07285"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit alpha"
                     /protein_id="HEY5739513.1"
                     /translation="MSVNEQIVADLVNNDISFVTTVPCKQLAGVIAGIEASSDILHIP
                     SNKEDEGMGLCAGAWMGGKRAAIIMQNTAIGVTINTLATLIQYYHIPLPMLISYRGEL
                     GEPVACQVEMAVHTKALLAQLNIPTYHFHHPDDVHELDAILKHCYMCNKPVAVLTDAS
                     FWKGAA"
     gene            1749..2534
                     /locus_tag="VIW27_07290"
     CDS             1749..2534
                     /locus_tag="VIW27_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5739514.1"
                     /translation="MNDNEYQEYDESLALLLEAIWGEGFMSPGGTDEVDRYLAGVEIE
                     GRRVLDIGCGLGGVDIHLLRGHRAAHVTGIDVDQSLIDRCRQLAQKYGLADSTEFVCV
                     EPGPLPFADASFEIVTSKDSIIHIEDKHALAADVFRVLEPGGWFVASDWLSGYEGETP
                     PEMQAYLDVEGLDFRLATPATYAAALEAAGFTDIEIDDRNEWYRGEARRERDHLRGAI
                     YEGLAPRLGSEFLERETRVWETMIVALDLGQLRPTHLRGRKPA"
BASE COUNT          496 a          846 c          826 g          487 t
ORIGIN      
        1 atagactcag cgtgatattc aggcaggcag tgccgtagac ccggaacagc atgccgagcg
       61 tctgcccggc gaggctgacc atcgccagcg aggccgcggc ggcgaatatc agcagcgcgc
      121 cgagggtata tacccagcgc cgcgacaggt ggcgaatcaa taccgggatc gaaaagctcg
      181 ccagcagccc ggttacgccg accgccgaga acaacaggct gacgtcgcgc gcgttgccga
      241 gcaactgcag cgcctgcacg gtgatcaccg tcgacagcaa tgcgcgcgcg taggattcga
      301 gcgcgtagag ggtcgcgaac acgcgcgccc cggggcgctc ttgcgctgtc tgcagccaca
      361 gcgggtaacg gacgtttatc ggcgggtcct ctgctgacga ttaaccggca aaccacctgt
      421 atagcaggat tggcgccacc cagccaaatt tcggcgccgc cagccgggcg cgccgtcaga
      481 aaagcgtcgg gttatcgcct tgcctcagcc gttgcgcttg cggatctcgt ccatgaagcg
      541 ctggcgaatc accaccgggt ccttgtcgat caccggcacc ttgatattgc cggactcaca
      601 cttgcagacg atgatcgtca ttcgctcgcc gtcgagcgcg gcacgcaccg cgtcggcggt
      661 atcctcggcg ctgcactcga ccacgttgtc gcaaccgcag gcggtggcga ccgccgccag
      721 cgaggttttt ctgccggcgt aggtcggctg gtcgccggtc gagccgtagc tgccgttatc
      781 gatgatcaac aggatgaaat tgtcggcgac gttgttggcg atcgtcggca gtgtgccgaa
      841 gttggtcaac accgagccgt cgccgtcgat ggcgatgacc ttttgcggct gcgccaacgc
      901 gaggccgagg ccgatcgacg acgccagccc catggtgccg agcatgtaga aattactcgg
      961 ctgatcgtcg agcatgtgca gttcctggct cggcaggccg atgttgcaga ccaccagctg
     1021 atcggagatc accggcacca gggatttaag gacttcgctg cgaatcatgc cgcccccttc
     1081 cagaaactgg catcggtgag caccgccacc ggcttgttgc acatgtaaca gtgcttgagg
     1141 atcgcgtcga gctcgtgcac atcgtcggga tggtgaaaat ggtaagtcgg aatattcaac
     1201 tgtgccagca aggccttggt atgcaccgcc atttccacct gacaggcgac cggttcgccg
     1261 agctcgccgc ggtaactgat cagcatcggc agcggaatat ggtaatactg gatcagcgtc
     1321 gccagcgtat tgatggtcac gccgatcgcg gtgttctgca tgatgatcgc cgcgcgcttg
     1381 ccgcccatcc aggcgccggc gcacagcccc atcccctcgt cttccttgtt ggacggaata
     1441 tgcaagatat cgctgctggc ctcgataccg gcgatcaccc cggcaagctg cttgcacggc
     1501 accgtggtaa cgaaggagat atcgttgttc accagatcgg cgacgatttg ttcgtttact
     1561 gacatgattc gtgattgggg ttactgggct cgaggcagta tcctgtttat ccatcaccat
     1621 gacaacatcc gcggaccgat atatgtcggc tacccgcgaa attcgctcgc cggcggcgcc
     1681 gacctacaat cgcgactttt aaaccggtta gcgacatcat atagttatcg aaaacggaaa
     1741 cccagggcat gaacgataac gaatatcagg aatacgacga aagcctggcc ctgctgctgg
     1801 aggcgatctg gggcgagggc ttcatgtcgc ccggcggcac cgacgaggtc gaccgctacc
     1861 tggccggtgt cgagatcgag ggccgccgcg tgctcgacat cggctgcggc ctcggtggcg
     1921 tcgatattca tctgctccgc ggccatcgcg ccgcgcacgt gaccggtatc gatgtcgacc
     1981 agtccctgat cgaccgctgc cggcagctgg cgcagaaata cgggctcgca gacagcaccg
     2041 agttcgtttg cgtcgagccc ggcccgctgc cgttcgccga cgccagcttc gaaatcgtca
     2101 ccagcaagga ctcgatcatt cacatcgagg acaagcacgc gctggccgcc gacgtgtttc
     2161 gcgtgctcga acccggcggc tggttcgttg ccagcgactg gctgtccggt tatgagggcg
     2221 agacgccgcc ggagatgcag gcctacctcg atgtcgaggg cctcgacttc aggctcgcca
     2281 cgccggctac ttacgccgcc gcgctagaag ccgcgggctt taccgatatc gaaatcgacg
     2341 accgcaacga gtggtaccgg ggcgaggcca ggcgggagcg tgaccacctg cgcggggcga
     2401 tctacgaggg ccttgcaccg cgcctcggca gcgagttcct cgagcgcgaa acccgggtct
     2461 gggagacgat gatcgtcgct ctcgatctcg gccagttgcg acccacccac ctgcgcggca
     2521 gaaagcccgc ctagccgcgc ctgccatgaa gaccgccgtt ttccgttgct gcgttttctg
     2581 cctgctcgcg atctcgctgc cggcgccggc acagtcgcca ccggcggtcg agccggtgcg
     2641 cgccatgctc gagct
//
LOCUS       DATCZV010000324         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_185161, whole genome shotgun sequence.
ACCESSION   DATCZV010000324 DATCZV010000000
VERSION     DATCZV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185161"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="proB"
                     /locus_tag="VIW27_07295"
     CDS             complement(<1..812)
                     /gene="proB"
                     /locus_tag="VIW27_07295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY5739515.1"
                     /translation="MNRYKKVVVKIGSSLITAGGKGLDHAAITGWAEQMAKLRAAGVD
                     VLLVSSGSIAEGITRLGLKSRPTTISELQATAAVGQMGLVQAYEACFQAHGLHSAQIL
                     LTHADLSNRRRYLNARTTLRTLLGYGTVPVVNENDSVSNEEIRFGDNDTLGALVANLI
                     EADLLIILTDQPGLFDADPDENPQAKLIERAVAGDPALNACAGDSSGGLGRGGMRTKV
                     SAAQLAARSGTHTVIVSGKEKDVILRVVSGEAVGSFIEATEGHLAARKQWLAG"
     gene            complement(799..1818)
                     /gene="obgE"
                     /locus_tag="VIW27_07300"
     CDS             complement(799..1818)
                     /gene="obgE"
                     /locus_tag="VIW27_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY5739516.1"
                     /translation="MKFIDEANIFVKAGDGGNGIVSFRREKYIPKGGPDGGDGGDGGS
                     VYVVGSKNLNTLSDFRHTRRFVARRGGNGKGNNRTGAKGDDVIIEVPLGTQITTLDHG
                     ELIADVVEAGQPMLIARGGFHGLGNTRYKSSTNRTPRQFSEGSKGEQFELHLELKLIA
                     DVGLLGMPNAGKSTLIRAVSAARPRVADYPFTTLHPNLGVVSVDRHRSFVIADIPGLI
                     EGAADGVGLGIRFLKHLQRTRLLLHIIDVMPGEGADSAVDSARKILRELQRYDESLYR
                     KPRWLVLNKLDLVPADQREALCAEIVAGLEWQAPVYTISALGRDGVDELCRDIMKYLE
                     DDESV"
     gene            complement(1897..2151)
                     /gene="rpmA"
                     /locus_tag="VIW27_07305"
     CDS             complement(1897..2151)
                     /gene="rpmA"
                     /locus_tag="VIW27_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5739517.1"
                     /translation="MAHKKAAGSTRNGRDSESKRLGVKKFGGEAVIPGNILVRQRGTK
                     FHAGDNVGCGKDHTLYAKAEGKVEFRVRGRHNRTFVSVVN"
     gene            complement(2161..2472)
                     /gene="rplU"
                     /locus_tag="VIW27_07310"
     CDS             complement(2161..2472)
                     /gene="rplU"
                     /locus_tag="VIW27_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5739518.1"
                     /translation="MYAVIATGGKQYKVTKGETLRVEKLDGDEGDTVKLDRVLMVADG
                     DKVSVGSPVLDNAAVTARIKAQGRGDKVEIIKFRRRKHSRSQAGHRQSFTEIEVTDIK
                     A"
     gene            2734..3228
                     /locus_tag="VIW27_07315"
     CDS             2734..3228
                     /locus_tag="VIW27_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5739519.1"
                     /translation="MQRHPHIVNRDEIEWTSDGNGKRFEFRRKWFTPATGARKLGCSL
                     YRVAAGKTAFPHHRHFANEEAIYVLAGEGKLRIDDTEVAVGPGDYIALPADGPAHQLI
                     NSGDNDLEYLCMSTMIHPDLTYYPDSDKIAAFAGSGPGGDKSVRTFNAVFRAGSAVDY
                     YDRE"
BASE COUNT          567 a         1071 c         1026 g          626 t
ORIGIN      
        1 ccggcgagcc attgcttgcg cgccgcgagg tgcccctcgg tcgcctcgat gaagctgccg
       61 acggcctcac cgctcaccac ccgcaggatg acgtctttct ccttgccgga gacgatcacc
      121 gtgtgtgttc ccgagcgtgc cgcgagctgt gcggcgctaa ccttggtgcg catgccgccg
      181 cgccccaacc ctccgctgct gtcgccggca caggcgttga gcgcgggatc gccggcgacg
      241 gcgcgctcga tcagcttggc ctgcggattt tcgtccggat cggcatcgaa cagccccggc
      301 tgatcagtca ggatgatcaa caggtcggcc tcgatcaggt tggcgaccag cgcgcccagc
      361 gtatcgttgt cgccgaaacg gatttcctcg ttggagacgc tgtcattttc attgaccacc
      421 ggcaccgtgc catagccgag caaggtgcgc agcgtggtgc gcgcgttgag gtagcggcgg
      481 cggttggaca ggtcggcatg ggtcagcagg atctgcgcac tgtgcaggcc gtgcgcctgg
      541 aaacaggctt cgtaggcctg caccagaccc atctgcccga ccgcggcggt cgcctgcagt
      601 tcggagatcg tcgtcggccg cgacttgagg ccgaggcgcg tgatgccctc ggcgatcgaa
      661 cccgacgaca ccagcagcac gtcgacgccc gcggcgcgca gctttgccat ctgctcggcc
      721 cagccggtga tcgccgcgtg atcgaggccc ttgccgccag cagtgatcag cgacgagccg
      781 atcttgacca cgactttctt ataccgattc atcgtcttcc agatatttca tgatatcgcg
      841 gcacagttcg tcgacaccgt cgcgaccgag cgcgctgatc gtgtagaccg gtgcctgcca
      901 ttcgaggccg gcgacgattt ccgcgcacag cgcctcgcgc tgatcggccg gcaccaggtc
      961 gagcttgttg agcaccagcc agcgcggttt gcgatacagc gattcgtcgt aacgctgcaa
     1021 ttcccgcaga atcttgcgcg ccgaatcaac ggcggaatcc gcgccttcgc cgggcatcac
     1081 atcgattata tgcagtagca gccgagtccg ctgcaaatgt ttgagaaagc gtatgccgag
     1141 accgacaccg tcggcggcgc cctcgatcag tcccggaatg tcggcgatca cgaagctgcg
     1201 gtgccgatcg acgctgacca cgccgaggtt cgggtgcagc gtcgtgaacg gataatcggc
     1261 gacccgcggc cgcgccgcgg agaccgcgcg gatcagcgtc gacttgccgg cattgggcat
     1321 gcccagcagg ccgacatcgg cgatcagctt cagctcgagg tgcagttcga actgttcgcc
     1381 cttgctgccc tcgctgaact ggcgcggcgt gcggttggtg gaactcttgt agcgcgtgtt
     1441 gccgagcccg tgaaaaccgc cgcgcgcaat cagcatcggc tgcccggctt cgaccacgtc
     1501 ggcgatcagc tcgccgtgat cgagcgtcgt gatctgggtg ccgagcggca cctcgatgat
     1561 tacgtcatcg cccttggcgc cggtgcggtt gttgcccttg ccattgccgc cgcgacgggc
     1621 gacgaagcgc cgtgtgtggc gaaaatcgga aagcgtgttg aggttcttgg agccgaccac
     1681 gtagaccgag ccgccgtcgc cgccgtcgcc gccgtcgggg ccgcccttgg gaatatactt
     1741 ttcgcggcga aaactgacga tgccgttgcc gccatcgccg gccttgacga aaatgtttgc
     1801 ttcgtcgata aatttcatcg cgggatcata ccaccggcta acgaaaaagc cccgcgaggg
     1861 cggggctttc gaatcggttt tcgcgggtga ctgtggctag ttgaccacgc tcacgaaggt
     1921 gcgattgtgg cggcccctga ccctgaactc gaccttgccc tcggccttcg cgtagagagt
     1981 gtggtccttg ccgcagccaa cgttgtcacc ggcatgaaat ttggtgccgc gctggcgcac
     2041 gaggatgttg ccaggaatca cggcctcacc accgaacttc ttcacgccga ggcgtttcga
     2101 ttccgagtcg cgaccgttgc gggtactgcc cgccgctttc ttgtgtgcca ttgcctgtac
     2161 ctatgccttg atatcggtga cttcgatttc agtaaagctc tgccgatggc cggcctgcga
     2221 acgcgaatgc ttgcggcggc ggaacttgat gatttcgacc ttgtcgccgc ggccctgggc
     2281 cttgatcctg gcggtcacgg cggcgttgtc gagcaccgga ctgccgaccg aaaccttgtc
     2341 gccgtcggca accatcagca cgcgatcgag cttgacggta tcgccttcgt caccgtcgag
     2401 cttttcgaca cgcaaggtct cgcccttggt gaccttgtac tgttttccac ccgtggcgat
     2461 cacagcgtac atgaggaact ccgaagcaat actgaaaaag gtggcgaatt ctagccgctc
     2521 gcgcggtgcc cgtcaatgct caaagcgatt gattccgcgg tttatccgct gcccgcaaca
     2581 gcgagtccgg gcgcccgtcg ccggctgtac cgggacggtg gccgggcgcc gccgtaaacc
     2641 gtcgaaatca aggtcgccgc cggcttgcag atcctttgat cgccgacggc aaggctttac
     2701 actggaaagc cgcccatcac aggagaccag gccatgcaac gtcatcccca tatcgtcaac
     2761 cgcgacgaga tcgaatggac cagcgacggc aatggcaaac gcttcgaatt tcgacgcaag
     2821 tggttcacgc cggcgaccgg cgcgcgcaag ctgggctgct cactgtaccg ggtggccgcc
     2881 ggcaagaccg cctttcccca tcatcggcat ttcgccaacg aagaggcgat ctacgtgctc
     2941 gcgggcgagg gcaagctgcg catcgacgac accgaggtcg cggtcggacc cggcgattat
     3001 atcgcgctgc cggccgacgg gccggcccac cagttgatca attccggcga caacgatctc
     3061 gagtacctgt gcatgtcgac gatgattcac cccgatctca cctattatcc cgattcggac
     3121 aagatcgcgg ccttcgccgg ctcggggccc ggaggcgaca agtcggtacg caccttcaac
     3181 gcggtcttca gggccggctc ggcggtcgat tactacgacc gagagtaaac cggccagtgg
     3241 cagtgctttc gtcgatcgcg gccaagggcc ggcacctggc gctgctggcc
//
LOCUS       DATCZV010000325         5255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_556742, whole genome shotgun sequence.
ACCESSION   DATCZV010000325 DATCZV010000000
VERSION     DATCZV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556742"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VIW27_07320"
     CDS             complement(<1..262)
                     /locus_tag="VIW27_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5739520.1"
                     /translation="MSLRMGIDLGGTKTEVIVLDARDEPVFRRRTDTPAHEYASILQM
                     IGELVAAARRATGDDFSIGLGIPGAISPQTGLLRNSNTACLNG"
     gene            792..3545
                     /locus_tag="VIW27_07325"
                     /pseudo
     CDS             792..3545
                     /locus_tag="VIW27_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018868548.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
     gene            3578..4771
                     /locus_tag="VIW27_07330"
     CDS             3578..4771
                     /locus_tag="VIW27_07330"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY5739521.1"
                     /translation="MLNWDEPLAPLKTRTPEPVIEPPAPASETAAPVVARQAPRVEEP
                     KPGLPRVDTPSLPEKPPLSAKPINPEDKRVVNGQTDINQLAPFKYPWAWEYFLNANRN
                     HWTPLDINMTQDVLDYQHKLTLEERHVYENVLSYLTTSDILAMRNIGLAVMEKMSAPE
                     LQIYQARQVYEEAMHTWTYQHCIETIGLDQGEIYNRYRVVPEIYHKIQMCNRRLDSVL
                     RPDIDLNDRDELENFVLAYTFFAGVFEGTWFYNGFSPIFALQRRGLMKGTAEQLQYIM
                     RDEVLHASFGIRVCNQIVQEEKVRIDPKVIQDMWEEAEAAEMQYARFLLPDPILGYSA
                     DDHLAQFRFIANRRANKLNHELPFPGAENALPWLDEQANLKKEKNFFETRVTEYQTGG
                     ALNWD"
     gene            5136..>5255
                     /locus_tag="VIW27_07335"
     CDS             5136..>5255
                     /locus_tag="VIW27_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017926146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY5739522.1"
                     /translation="MSISALPLTSPPQKLCIIRLSALGDVTHAVPVLRAIQQQW"
BASE COUNT         1171 a         1662 c         1475 g          947 t
ORIGIN      
        1 ggccgttcaa acaggcggtg ttcgaattgc gcaacaaccc ggtttgcggc gagatggcgc
       61 cggggatgcc gagaccgata ctgaaatcgt cgccggttgc tcgccgcgcg gccgcaacca
      121 gctcgccgat catctgcaga atcgacgcat actcgtgtgc cggggtatcg gtccgtcgcc
      181 gaaataccgg ctcatcccgc gcatcgagca cgattacctc ggtcttggtc ccccccagat
      241 ctatccccat tctcagactc atttcacgcc tccgcgatgg tcccgcacgg gctattcata
      301 taaatttttt atgattttat tcacaaaata atactttttt ataattcaaa aattgcggtg
      361 ataaaaatca atgagctaga agcctttgct gtggccgttt ctaaccgata atcaccactt
      421 atccacagct tatacccgca ctctccacag aatcgtacac aacataatgt acttgacctg
      481 accccaaaac acattatatt gtgtatacga ttaattaata ccttaaaaga gctaaggctg
      541 gccggacaag tccggatcct cgtcgaaaac gtattcgaat aattcgagag ttaataatcc
      601 actttaactt tcgacgggaa gcatccagcc ttagttggta aacgcggatt aaatccagtc
      661 acccacaaga gaaacacgga ccgggctagt taatgcaaaa catcgaatct cccctgacag
      721 aggcagccgt cgcgcctgcc gacaccgcaa ccgtcgcgac cgaggataac agtggcgcgc
      781 tgaccagcaa ctacaacgtg gtgcgccgca acggcaagct gaccagtttc gacaagaaca
      841 aaatcgcgat cgcgatgacc aaggctttcc tggccgtcga gggcggacag gccgcggcct
      901 cgtcccgggt tcacgaaact gtcgacaaac tgaccgacct ggtggtcgcc acgctgaccc
      961 gtcggcagcc cgatggcggt accttccata tcgaggacat ccaggaccag gtcgaactgg
     1021 cactgatgcg aaacggcgag caaaagatcg cgcgcgccta cgtgctgtat cgcgaggagc
     1081 gcgccgcggc acgcgcgcaa tcgaagcagg cgcaaccgca gcagggcgac gacgaggagc
     1141 gcatcgaact ctcggtaaag ctcgacgacg gcagcttcgt gccgctcgat accgcgcgcc
     1201 tgaggacggt catcgacgag gcctgcagcg gcctcgagag cgtcagctcg gaacgcatat
     1261 ataacgacac catgaagaac ctgttcgacg gcgtcgccgc ggccaacgtc agccccacgg
     1321 cggtgatgag cgcgcgcgca atgatcgacc gcgagcccga gtactcgcag gtcgccgcgc
     1381 gcctgctgct cgacgagctg cgcgccgaat ccctcggctt catcaacgac ggcgcgccga
     1441 gcgcgacctt cgcggaaatg accgagcgct acggcgaata cttcgccaac ttcgtcaagc
     1501 aggcgtccga cctcgaattg cttgaccgcg aactgctcaa gttcgacctc gagcgcctcg
     1561 gcaacgcgtt acgacccgag cgcgacctgc agttcaccta cctcgggctg caaaccctct
     1621 acgaccgcta cttcatccac cacagcgata cccgtttcga gttgccgcag gcatttttca
     1681 tgcgcgttgc gatgggtctg gcgatcaacg aggtgcagcg cgaagagcgc gcgatcgagt
     1741 tttacgacct gttgtcgtct ttcgatttca tgagcagcac cccgacgctg ttcaactcgg
     1801 gcacgctgcg cccgcagctg tcgagctgct acctgaccac cgtgcccgac gatctcgacg
     1861 gtatcttcag cgcgatcaag gacgatgccc tgctgtcgaa gttcgccggc ggcctcggca
     1921 acgactggac ccgggtgcgc ggcatgggtt cgcatatcaa gggcactaac ggcaagtcgc
     1981 agggcgtggt gccgttcctc aaggtcgcca acgataccgc ggtcgcggtc aaccagggcg
     2041 gcaagcgcaa gggcgcgatg tgcgcctacc tggaaacctg gcacctcgat atcgaggatt
     2101 tcctcgatct gcgcaagaac accggtgagg agcgccgccg cacgcacgat atgaacaccg
     2161 ccaactggat tccggacctg ttcatgaagc gggtcgccga ggaaggccag tggacgctgt
     2221 tctcgccctg cgaggtgccg gaactgcacg atctctacgg ccaggaattc gaaaccgcct
     2281 atgtcgccta cgaagaaaag gccgatcgcg gcgaaatcaa gaatttccgc cgcatcaagg
     2341 cgcaggacct gtggcgcaag atgctcggca tgctgttcga gaccggccac ccgtggatga
     2401 ccttcaagga cccgtgcaac attcgctcgc cgcagcagca catcggcgtc gtgcattcgt
     2461 ccaacctgtg taccgagatc acgttgaata cctcggacac cgaaatcgcg gtctgcaacc
     2521 tcggttcggt caacctcgcg cagcacgtga acgaaaacgg tctcgacctg gccaagctcg
     2581 agaaaaccat cggcaccgcg atgcgcatgc tcgacaacgt catcgattac aactactaca
     2641 gcgtgccgca ggcgcgcacc tcgaacctgc gacaccgccc ggtcggtctc ggcgtgatgg
     2701 gcttccagga tgcgctctac aagctgcaca ttgcctacag ctccgaagac gcgatcgagt
     2761 tcgccgatga atcgatggaa gcggtcagct actacgcgat ccaggcctcg acccagctgg
     2821 ccgaggaacg cggcagctac tcgagctttc aagggtcgct gtggagccag ggcatcctgc
     2881 cgatcgactc ggtggatcga ctgcgtcagg tgcgcggcga ttacctgcag caggacggca
     2941 gttcgcgcat gaactgggac agcctgcggc agcgggtgat gggcgtcggc atgcgcaatt
     3001 cgaacaccat ggcaattgcg ccgaccgcga ccatatccaa catctgcggc gtcagccagt
     3061 cgatcgagcc gatctaccag aacctgttcg tcaagtccaa cctgtcgggc gagttcacag
     3121 tcgtcaatcc ctacctggtc gaggacctca agcagcaagg actgtgggac gatgtcatgg
     3181 tcaacgatct caagtactac gatggcagcg tgcagcatat cgaccgcgtg ccgtcggcgc
     3241 tgaaggcgat ttacgccacc gccttcgagg tcgattcacg ctggctggtg gaagccgcgt
     3301 cgcgccggca gaaatggatc gaccaggggc aatcgctgaa cctgtacatg gccgagcctt
     3361 ccggcaagag gctcgacaat ctgtacaagc tcgcctgggt gcgcggcctg aaaaccactt
     3421 attacctgcg ctcgctgggc gcaaccgcgg tggagaaaaa ctcggcccgc gaacccgagc
     3481 cgaaaatcga ggcggaaccc aaggtttgct cgatcctcga tccggattgc gaagcctgtc
     3541 aataaacaag ctgtagataa agaagtagga gaaacaaatg ctgaactggg atgaaccgct
     3601 ggccccgctg aagacccgca caccggagcc ggtcatcgag ccgccagcgc ccgctagcga
     3661 aaccgcggcg cccgtcgtcg cacggcaagc gccgcgcgtc gaggaaccta agcctggcct
     3721 gcctcgcgtc gatacgccga gcctgccgga aaaaccaccg ctctcggcca agccgatcaa
     3781 tcccgaggac aagcgagtcg tcaacggcca gaccgacatc aaccagctgg cgccgttcaa
     3841 gtacccctgg gcctgggagt atttcctcaa cgccaaccgt aatcactgga cgccgctgga
     3901 catcaacatg acccaggacg tgctcgatta ccagcacaag ctgaccctcg aggagcgcca
     3961 cgtctacgaa aacgtgctct cttacctgac gacctccgac atactggcga tgcgcaacat
     4021 cggcctcgcg gtgatggaaa agatgtcggc gcccgaactg cagatctacc aggcgcgcca
     4081 ggtctacgag gaagccatgc atacctggac ctaccagcac tgcatcgaga ccatcggcct
     4141 cgaccagggc gagatttaca accgctaccg cgtggtgccc gagatatatc acaagatcca
     4201 gatgtgcaac cgtcgcctcg actcggtact gcgcccggat atcgatctca acgaccgcga
     4261 cgaactggaa aacttcgtgc tcgcctacac ctttttcgcc ggggttttcg agggcacctg
     4321 gttctacaac ggcttcagcc cgatcttcgc actgcagcgg cgcggtctga tgaagggtac
     4381 cgccgagcaa ttgcagtaca tcatgcgcga cgaggtactg cacgcgtcgt tcgggattcg
     4441 tgtctgcaac cagatcgtgc aggaagaaaa ggtcaggatc gacccgaaag tcatccagga
     4501 tatgtgggaa gaagccgagg ccgccgagat gcagtatgcc cgcttcctgc tgccggaccc
     4561 gatcctcggc tacagcgccg acgatcatct cgcgcagttc cgctttatcg ccaaccgccg
     4621 cgccaacaag ctgaaccacg agctgccgtt cccgggagcc gagaacgcgc tgccgtggct
     4681 cgacgaacag gccaatctaa agaaggagaa aaactttttc gagacccgcg tcaccgagta
     4741 ccagacgggt ggcgcgctca actgggatta gatttgactg gcaattctcg cgtaagtcga
     4801 agttacgcga tgaatgcaag atgcccgctt tcgcggggac atggattagt gttacacgcg
     4861 ctcttcggtt gtaaccttgg gccggttagc gggaaccttc aattcccgct aaccggcctt
     4921 ttttatcggc ctgcccgcat tgctcgacga tttcctgccg cggtcgccgt aaagcgcact
     4981 gcgacatgcc gtgccgccga tggctggctt gccaaagtgt tgaccgcagc agtgccggcc
     5041 tctgatccgc ggcgcttgcc acagcgtctt ttcagggccg tcgcgacaga catcggttaa
     5101 tattgcggtc caaggtaacc tgattcccgg caaccatgag tatctccgcc ctgcccctga
     5161 cctcgccgcc gcaaaagctt tgcatcatcc gcctgtcagc gctcggcgac gtgacccacg
     5221 cggtaccggt gctgcgcgcg atccagcagc agtgg
//
LOCUS       DATCZV010000326         6972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_396612, whole genome shotgun sequence.
ACCESSION   DATCZV010000326 DATCZV010000000
VERSION     DATCZV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396612"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VIW27_07340"
     CDS             <1..240
                     /locus_tag="VIW27_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739523.1"
                     /translation="DLRLLELLKSDARGELEPVLLDIDSRLLRPLLSARRGRRGPLLL
                     RAGFDCDFYLPPSANWKFWRRPRSLADWMATRVNL"
     gene            303..1481
                     /locus_tag="VIW27_07345"
     CDS             303..1481
                     /locus_tag="VIW27_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY5739524.1"
                     /translation="MSKKKPERSFATVEAVVEELKPGYPVYCLRPAELKRQAGFFLDN
                     FPGRVMYAVKCNPHLAVLKALYEAGIRHFDTASLAEIALVREHFPQADCYFMHPVKPR
                     AAIRTAHDVYSVDHFVIDHDKELDKIIATTGGGDGQVVLVRIITPVHDAQYQLSDKFG
                     IAAEDAPALLQKVHKADFQTGVAFHVGSQCRNPQAFVDGVNTALDVIQAAGVPVHYLD
                     VGGGFPALYEDDHPPDLRAYFDSIHMAFDESRLRRDCVLMCEPGRALVASGCTLLTQI
                     QLRKENKLYINDGVYQSLSETVAGKFRLPARLINPWREIASECQDYTILGPTCDNLDI
                     LPAPFHLPVDAEEGDWIEIGQVGAYSNSAATRFNGFFPETFVSVEAPPLLPAVAEMKR
                     "
     gene            complement(1550..2287)
                     /locus_tag="VIW27_07350"
     CDS             complement(1550..2287)
                     /locus_tag="VIW27_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739525.1"
                     /translation="MTQDVDIQLKVWKDLAISKQILMGAATDALGLDAECSTEELKQA
                     LDEAIQRARDADITIQQTRSQAEQQIAEYRQKMEAAEAARKRAEEQAATAVKTRAQAE
                     HQLAQGKEDNVAALKKARAEVTDKQNQLKAISKKLADTPENVVRKLKTLKKQKLDESK
                     ARAQVESKLQKERKQNVKLESEVESKRALLKSAAGLVGQVRKLHQACVEAAETIAGLS
                     DKKKDQLKVPEVDLEALEHLEKALSGK"
     gene            2387..3512
                     /gene="prfB"
                     /locus_tag="VIW27_07355"
     CDS             join(2387..2488,2490..3512)
                     /gene="prfB"
                     /locus_tag="VIW27_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638028.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5739526.1"
                     /translation="MRVFRTVAASVETNYISQQIDDLESRVDSLRGYLDYDQKREKLE
                     EVMLELENPKIWSDPEHAQQLGREKAALEGIVGGLDQASQALAEAAELLELAIGEDDA
                     ATAAEVEADLEAVETTVAGLEFRRMFSGEMDTHNAYLDIQSGAGGTEAQDWAEMLLRM
                     YLRWGEAKGFKTELIEVSEGDVAGIKSATVRFEGPYAYGWLRTETGVHRLVRKSPFDS
                     GNRRHTSFASAFVSPEVEDDIDIEIDPSDLRIDVYRASGAGGQHVNRTESAVRITHLP
                     TNVVVQCQNDRSQHKNKATAMKQLKAKLYELEVQMRNAKAKSIEDLKSDIGWGSQIRS
                     YVLDQSRIKDLRTGVETGNTQGVLDGDLDPFIEASLKSGA"
     gene            3532..5031
                     /gene="lysS"
                     /locus_tag="VIW27_07360"
     CDS             3532..5031
                     /gene="lysS"
                     /locus_tag="VIW27_07360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5739527.1"
                     /translation="MDDENPYIEQRREKLARLRADGQAYPNGFEREDFAAEIRERYEA
                     KSKEELADQAARVQMAGRMMLRRDGGKAIFADLQDMSGRMQIFVRVDTLGEEAFEAFK
                     HTDIGDIFGVCGEVFKTNRGELSIKVEQLQLLSKSLRPLPEKYHGLTDHEQKYRQRYV
                     DLIMNEDSRRVFLIRSKTVQFIREYMIAAGFVEVETPMMQVIPGGAVARPFVTHHNAL
                     DMELYLRVAPELYLKRLVVGGIEQVFEINRNFRNEGLSTRHNPEFTMLEFYQAYADYE
                     DFIDLTEDMMRKLAEAVLGSQTIVYQGEEYDLSAPFARMTVAEAILKYNPELDAGRLE
                     DVDYCREAARRLGIEPEESWGAGKLQIEIFEKTVEDQLQQPTFVTAYPTEISPLSRCN
                     DENPFVTDRFEFFIGGQELANGFSELNDPEDQAARFRRQVEDKEAGDMEAMHFDADYI
                     RALEYGMPPAAGEGIGIDRLVMVLTDSPNIRDVILFPHLRPESGLETGD"
     gene            5522..>6972
                     /locus_tag="VIW27_07365"
     CDS             5522..>6972
                     /locus_tag="VIW27_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5739528.1"
                     /translation="MNTLAAAPGFHAYAVMVLVVLALLLFASERVPLPTSSLLILVLL
                     TLLFEIFPYSGESGRLRASDLFLGFGHQALVAVCALMIAGQALVRTGALEPIGRQLAR
                     WWTQSPRLAFLAALVIGAVLSAFVNNTPVVILLLPILISISLKTAKPASDLLMPLGLA
                     TSVGGMATTIGTSTNLLVVAVAADLGLRELGMFDFAFPALVAGSAAILYLWLIAPLLL
                     PEREARLPTTSPRLFTAQLRIHDDSVANQKKMAEVIDLAGGNLEVVHIIRGKKNNRLV
                     AIPDMVLRSGDRIIVRSTPDRLKELETVLGASLFSGDERVDDEHPLRDTTQQIAEVVV
                     VPGSTLDGRTLANIRFRERYQLMVLALYRSNKWASKQGTEHTEETRLHSGDVLLVQGS
                     INNIKRLKSSRNLLVLDAKAALPISSRAPLAMLIMLGIIVSAALGLLPIAVSALCGVL
                     LMLLSGCIKWFDATRALSAPVVLVIVTSLAMGAA"
BASE COUNT         1273 a         2117 c         2204 g         1378 t
ORIGIN      
        1 gatctgcggc tgctcgaatt gctcaaatcg gacgcgcgcg gtgaactcga acccgtgctg
       61 ctggacatcg acagccgcct gctacgcccg ctgctgtcgg cgcgccgcgg gcgtcgcggg
      121 ccgctgctgt tgcgcgcggg attcgactgc gatttttacc tgccgccgtc ggcgaactgg
      181 aaattctggc gccgtccgcg cagtctggcg gactggatgg caacgcgggt taacctctga
      241 tttgagttgt tgactaacgc gacagggtgc gcgaatctgt gtttttccaa gaggattgac
      301 cgatgtcgaa gaaaaagccg gaacgctcgt tcgccacggt cgaggccgtg gtcgaggagt
      361 taaaacccgg atatccggtt tactgcctgc gacccgctga actgaagcgc caggccggtt
      421 ttttcctcga taactttccc ggtcgcgtga tgtacgcggt caagtgcaac ccgcatctgg
      481 cggtactcaa ggcgctctac gaggcgggta ttcgccattt cgataccgcc tcgctggcgg
      541 aaatcgcgct ggtccgcgag cattttccgc aggccgattg ctacttcatg cacccggtga
      601 aaccgcgcgc cgcgatccgt accgcccacg atgtctattc ggtggatcat ttcgtgatcg
      661 atcacgacaa ggaactcgac aagatcatcg ccactaccgg cggcggcgac ggccaggttg
      721 tactggtgcg tatcatcacg ccggtgcacg atgcgcagta ccagttgtcg gacaagttcg
      781 gtatcgccgc ggaggatgcg ccggcactgc tgcaaaaggt tcacaaggcc gattttcaga
      841 ccggtgtggc gtttcatgtc ggctcgcagt gccgcaatcc gcaggctttc gtcgacggcg
      901 tcaataccgc gctcgatgtc atccaggctg ccggcgtacc ggtgcactac ctcgatgtcg
      961 gtggtggctt cccggcgctc tacgaagacg atcatccgcc cgatctgcgc gcctatttcg
     1021 attcgataca catggccttc gacgaatcac gcctgcgccg cgactgcgtg ctgatgtgcg
     1081 agcccggtcg cgcgctggtc gccagcggct gcacgctgct gacacagatt caattgcgca
     1141 aggaaaacaa gctttatatc aacgacggcg tgtaccagag tctgtcggaa accgttgccg
     1201 gcaagttcag gctgccggcg aggctgatca acccgtggcg ggaaatcgcc agcgaatgcc
     1261 aggactacac gatcctcgga cccacctgcg acaacctcga catattgccg gcgccgtttc
     1321 atctgccggt ggacgcggaa gagggtgact ggatcgagat cggccaggtc ggggcttaca
     1381 gcaattcggc ggcgacgcgc ttcaacggct tttttccgga gaccttcgtc agcgtcgagg
     1441 cgccgccgct gttgccggcg gtggcggaaa tgaaacgctg atggtgccgg cgggcgcagc
     1501 gcgcagcctg gacggacgcc ggcctgcgat tagcgggaaa aactgacggc tatttgccgg
     1561 agagcgcttt ttcgaggtgc tcgagggctt cgaggtcgac ctcgggtacc ttgagctgat
     1621 ccttcttctt gtcgctgagt ccggcgatgg tttcagcggc ctcgacacag gcctggtgta
     1681 gcttgcgcac ctgcccgacc agcccggcgg cgctcttcag cagggcccgc tttgattcga
     1741 cttccgactc gagtttgacg ttctgcttgc gctccttctg cagcttcgac tccacctgcg
     1801 cgcgcgcctt ggattcgtcg agtttctgct tcttcagcgt cttcagcttg cggaccacgt
     1861 tttccggggt gtcggcgagt tttttcgaga tcgccttgag ctgattctgc ttgtcggtga
     1921 cctcggcacg cgcttttttc agcgccgcga cattgtcttc cttgccctgg gcgagctgat
     1981 gctcggcctg agcgcgcgtc ttgacggcag tggcggcctg ctcctcggcc cgcttgcggg
     2041 ccgcttccgc ggcctccatc ttttgccgat actcggcgat ctgctgttcc gcctggctgc
     2101 gggtttgctg gatggtgata tcggcgtcgc gggcgcgctg gatcgcttcg tcgagcgcct
     2161 gcttgagttc ctcggtgctg cattcggcgt ccagaccgag cgcgtcggtg gccgcgccca
     2221 tcaggatctg cttgctgatt gcaaggtctt tccagacctt taactgaata tcaacatctt
     2281 gtgtcactgc ttaactcgcg tctgaaattt ggcggcacat tctagcccaa cggggcgcgg
     2341 aaaaataggc tttgtcgggt gatattatcg ccggggatcg ggtagaatgc gcgtttttcg
     2401 aacggtggcg gccagcgtgg aaaccaatta catcagccag caaatcgacg acctcgaatc
     2461 gagggtcgat tcgctacggg ggtatctttg actacgacca gaagcgcgag aagctggaag
     2521 aagtcatgct cgaactggaa aatccgaaga tctggagcga cccggaacac gcgcagcaac
     2581 tggggcgcga aaaagccgct ctcgagggca tagtcggcgg tctcgatcag gcctcgcagg
     2641 cgctcgccga ggcggccgaa ttactcgagc tggcgatcgg cgaagacgac gcggcgaccg
     2701 ccgccgaagt cgaggccgat ctcgaagcgg tcgagacgac cgtcgccggg ctcgagttcc
     2761 ggcgcatgtt ctcgggtgag atggataccc acaacgctta tctcgatatc cagtcgggcg
     2821 ccggcggcac cgaggcgcaa gactgggccg aaatgctgct gcgcatgtac ctgcgctggg
     2881 gcgaggccaa gggtttcaag accgaattga tcgaggtctc ggagggcgac gtggccggca
     2941 tcaagagtgc cacggtgcgc ttcgagggcc cctacgccta cggctggctg cgtaccgaga
     3001 ccggcgtgca ccggctggta cgcaagtcgc cgttcgactc cggcaatcgc cgccacacgt
     3061 cgtttgcgtc ggccttcgtg tcgccggaag tcgaggacga tatcgacatc gagatcgacc
     3121 cgtcggacct gcgtatcgac gtctaccgtg ccagtggcgc cggcggccag cacgtcaacc
     3181 gcaccgagtc ggcggtgcgt atcacccacc tgccgaccaa cgtcgtggta cagtgccaga
     3241 acgatcgctc gcagcataag aacaaggcca ccgcgatgaa gcagttgaag gcgaagcttt
     3301 acgaactcga ggtgcagatg cgcaacgcca aggccaagag catcgaggat ctgaaatcgg
     3361 acatcggctg gggcagccag attcgctcct acgtgctcga ccagtcgcgt atcaaggatt
     3421 tgcgtactgg cgtcgaaacc ggcaacactc aaggcgtgct ggacggcgac ctcgatccct
     3481 tcatcgaggc gagcctgaaa agcggcgcct gatcaaacag cgagaaccca gatggatgat
     3541 gaaaatcctt acatagagca gcggcgtgaa aagctcgccc gcctgagggc ggacggccag
     3601 gcatatccga acggttttga acgcgaggat ttcgccgccg aaatccgtga gcgctacgag
     3661 gcgaagagca aggaagaact cgccgaccag gcggcgcggg tgcagatggc cgggcgcatg
     3721 atgctcaggc gagacggcgg caaggcgatc ttcgccgacc tgcaggacat gagtggccgc
     3781 atgcagatat tcgtgcgcgt cgatacgctc ggcgaggaag ccttcgaggc cttcaagcat
     3841 accgacatcg gcgacatttt cggcgtctgc ggcgaggtgt tcaagaccaa tcgaggcgaa
     3901 ctcagcatca aggtagagca gctgcagttg ctgagcaagt cgctgcggcc gttgccggaa
     3961 aaataccatg gtctgaccga tcacgagcag aagtaccgcc agcgctatgt cgacctgatc
     4021 atgaacgagg attcgcgccg cgtcttcctg atccgcagta aaacggtgca gttcattcgc
     4081 gagtacatga tcgccgccgg tttcgtcgaa gtcgaaacgc cgatgatgca ggtgataccc
     4141 ggtggcgccg tggcgcggcc cttcgtgacc caccacaatg ccctcgacat ggaactctac
     4201 ctgcgcgtcg cgcccgagct ttatctcaag cgcctggtgg tgggcggtat cgagcaggtt
     4261 ttcgaaatca accgcaactt ccgcaacgaa ggcctgtcga cgcggcataa cccggaattc
     4321 acgatgctcg agttctacca ggcctacgcc gattacgagg atttcatcga cctgaccgag
     4381 gacatgatgc gcaagctcgc cgaggccgtg ctcggttcgc agaccatcgt ttaccagggt
     4441 gaggaatacg acctgtcggc gccgttcgcg cgcatgacgg tggccgaggc gatcctcaaa
     4501 tacaacccgg aactcgacgc cggtcggctc gaggacgtcg attactgccg cgaggcggcg
     4561 cggcgtctcg gtatcgagcc cgaagagagc tggggcgcgg gcaagctgca gatcgagatt
     4621 ttcgagaaaa ccgtcgagga ccagctgcaa cagccgacat tcgtcaccgc ttaccccacc
     4681 gagatttcac cattgtcacg ctgcaacgac gaaaacccgt tcgtcaccga ccgctttgaa
     4741 tttttcatcg gcggccagga gctcgccaac ggcttttccg agctcaacga tcccgaggac
     4801 caggccgcac gtttccgccg ccaggtcgaa gacaaggagg ccggcgatat ggaagccatg
     4861 catttcgacg ccgattacat tcgcgcgctg gagtacggca tgccgccggc ggcgggcgag
     4921 ggcatcggta tcgaccgcct ggtgatggtg ctgaccgatt cgccgaatat tcgcgacgtg
     4981 atcctgtttc cgcacctgcg gccggaatca gggttggaaa ctggcgacta aaatcgaaga
     5041 gtttcccgat cacccgaggg gtacttggga aatggctcct ggctggcgcg attcgttgtt
     5101 cgaggttcgg cgcttcgctt gattgtgggc ctcggcaagc ccgcttaaac gggcttgcat
     5161 cggggccttc gctgcgctcg aggcaaggtc gcttcgaggg ggtcagtgca aaatttcccc
     5221 gagcagtggg atttcgatta aagcctgacg cagtccttgc gcagacgagg attgtgccga
     5281 ctggcggtgg cattagtcgg gtcgtgctgg aaaatcctga tctgaccccg agggtccctg
     5341 gagtcggtcc cgcgcaagcg ggaatcttct gcctcgcgcg ggcgaggctt tctccggccg
     5401 cgctggcgtt agccgacgcg ttcgtggaaa ttttcccttg ccgccgaatg ttcgagcatc
     5461 gtgtcgcgct aatcggtgga agtgctagaa taattcgcac cctcgaatcg attccgccct
     5521 catgaacact ctcgctgccg ctcccgggtt tcacgcctat gccgtgatgg tgctggtggt
     5581 gctggcactg ttgctgttcg ccagcgagcg ggtgccgctg ccgacctcga gcctgctgat
     5641 cctggtactg ctgacactgc tgttcgagat ttttccgtat agcggcgagt ccggccgact
     5701 acgcgccagc gacctgtttc tcggcttcgg gcaccaggca ctggtcgcgg tatgcgcgtt
     5761 gatgatcgcc ggccaggcgc tggtgcgtac cggtgcgctg gagccgatcg gccggcaact
     5821 ggcgcgctgg tggacgcaaa gcccgcggct ggcgtttctc gccgcgctgg taatcggcgc
     5881 ggtgctcagt gcttttgtca acaacacccc ggtggtcatc ctgttgctgc cgatcctgat
     5941 cagcatctcg ctgaagaccg cgaagccggc ctccgacctg ctgatgccgc tcgggctggc
     6001 gacctcggtc ggcggcatgg cgaccactat cggtacctcg accaacctgc tggtggtcgc
     6061 ggtcgccgcc gacctcggcc tgcgcgagct gggcatgttc gatttcgcgt ttccggcgct
     6121 ggtcgccggc agcgccgcga tcctttacct gtggctgata gcgccgctgt tgctgccgga
     6181 acgcgaggcg cgcctgccga ccacgtcgcc gcgcctgttc acggcgcaat tgcgcattca
     6241 cgacgacagc gtcgccaacc agaagaagat ggccgaggtt atcgatctgg ctggcggcaa
     6301 cctcgaggtc gtgcatatca ttcgcggcaa gaaaaacaac cgcctggtag cgatcccgga
     6361 catggtgctg cgcagcggcg accgcatcat cgtcaggagc actcccgatc gcctcaagga
     6421 actcgaaacc gtgctcggcg caagcctgtt ctccggcgac gagcgagtcg acgacgaaca
     6481 cccgctgcga gacaccacgc agcaaatcgc cgaggtcgtc gtggtgccgg gctcgacgct
     6541 cgatggccgc acgctcgcca atattcgttt tcgcgaacgc taccagctga tggtgctggc
     6601 gctctaccga tccaacaagt gggccagcaa gcagggcacc gagcataccg aagagacgcg
     6661 cctgcactcc ggcgatgtgc tgctggtgca gggctcgatc aataatatca agcgcctcaa
     6721 gtcgagccgc aatttactgg tactcgatgc caaggcggcg ctgccgatct cgtcgcgcgc
     6781 gccgctggcg atgctcatca tgctcggcat aatcgtcagt gcggcgctcg ggctgctgcc
     6841 gatcgcggtc agtgcattgt gcggcgtgct gctgatgctg ctgagcggct gcatcaagtg
     6901 gttcgatgcc acgcgtgcgc tgagcgcccc ggtggtgctg gtgatcgtca ccagcctggc
     6961 gatgggcgcg gc
//
LOCUS       DATCZV010000327         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_132126, whole genome shotgun sequence.
ACCESSION   DATCZV010000327 DATCZV010000000
VERSION     DATCZV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132126"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="VIW27_07370"
     CDS             complement(<1..1803)
                     /locus_tag="VIW27_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5739529.1"
                     /translation="MKLQAIILLSAGLLSAVAAAQDSGLGPITVTATRVEKNLDEIPA
                     AVSVVGQDDIQLGTQQLGLDESLGGVPGLFMQNRYNFAQDLRASIRGFGARSSFGIRG
                     IKIIVDGIPETLPDGQGSVDGIDLGSAREITVIRGPTSSLYGNASGGAILVESEKGSL
                     VPFASGRVTVGDYDFRNAQIKLGGDTGRVNYLFNLSDTSTDGYRDHSEFENTQLNARV
                     ETQLEGDSSLLTTLHYTDQPLANDPGGITAEDAADDPTQARDRNEEYDAGEALEQTRI
                     GLLYNTAVDADRDLEARVYHVGRDFSNKLPFESGGSVDLERSFVGTGLKYTHRGQLAA
                     RSNRLLLGFDYDRQDDDRRRFDNLQGEIGDKVFDQNELVTAFGAYLQNETRLSDTVEL
                     TAGLRYDEIEFDVSDDYLADGDDSGKVDFDHVSPMVGVSVKRGANTHFYATVSTAFET
                     PTTTEFANPDGGGFNQSLEPQDSTNYEIGVKQQTATYRFELALFHIDVDDELTPFELP
                     SQPGRTFFENAGSSTRDGIEIAYSRQLARQLGFSAAYTYSDFVFDRFTNEDGDVFDGN
                     QIPGIPEDLLYLELNWFGDAGYYAKWDVTYTGELY"
     gene            complement(1919..>2610)
                     /locus_tag="VIW27_07375"
     CDS             complement(1919..>2610)
                     /locus_tag="VIW27_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5739530.1"
                     /translation="RPVGFVAMQTVTRILARLKLAPCLMAVVTLCLAPAAVAGEDVDD
                     GRIKVEQVCANCHGLYGEAASGGNSALSPKLTAQNREYLVSRLLDYRSGKREHAQMTF
                     VAQMISEQDIDNVAAWYAGLQIDLSGNLDIADEALEQSERAGKARAEQLCQHCHGLDG
                     QAVVSSSSSPVPDLSGQPRPYIVERLKAYRSRKIEHPQMTPIAKSLTDADIANVADWY
                     SGIRVEVLDFD"
BASE COUNT          466 a          792 c          824 g          528 t
ORIGIN      
        1 gtaaagctcg ccggtgtagg tcacgtccca cttcgcgtaa tagccagcgt cgccgaacca
       61 gttgagttcg aggtagagca ggtcctcggg aatccccggg atctggttgc cgtcgaacac
      121 gtcgccgtcc tcgttggtaa agcggtcgaa gacgaaatcg gaataagtat aggccgccga
      181 aaaaccgagc tgcctggcga gctgccgcga ataggcgatc tcgatgccgt cgcgggtcga
      241 cgagccggcg ttctcgaaga atgtccgccc cggttgcgac ggcagttcga aaggtgtcaa
      301 ttcgtcgtcg acgtcgatgt gaaacagtgc cagctcgaag cggtaggtcg cggtctgttg
      361 cttgacaccg atttcgtaat tggtcgaatc ctgcggctcg agcgactggt tgaagccgcc
      421 gccgtcggga ttagcgaact cggtagtggt cggggtttcg aacgcagtgg acacggtcgc
      481 gtagaaatgg gtattggctc cacgcttgac gcttaccccg accatcgggc tgacgtgatc
      541 gaaatcgacc ttgcccgaat cgtcgccgtc ggcgaggtaa tcgtcactga cgtcgaactc
      601 gatctcgtcg tagcgcaggc cggcggtcag ctcgacggtg tcgctgagcc gggtctcgtt
      661 ctgcaggtaa gcaccgaacg cggtcaccag ttcgttctga tcgaagacct tgtcgccgat
      721 ctcgccctgc aggttgtcga aacggcggcg atcgtcgtcc tggcggtcgt aatcgaaacc
      781 cagcagcaga cggttcgacc tggcggcgag ctgtccacgg tgggtgtact tgagaccggt
      841 accgacgaaa ctgcgctcga ggtcgaccga gccgccgctt tcgaacggca gcttgttgct
      901 gaaatcccgg ccgacgtggt agacccgcgc ctcgaggtcg cggtcggcat cgactgcagt
      961 gttgtacaac agcccgatgc gcgtctgctc cagcgcctcg ccggcatcgt attcttcgtt
     1021 gcggtcgcgt gcctgcgtcg gatcatccgc cgcatcctcg gcggtgatac cgcccggatc
     1081 gttggccagg ggctggtcgg tataatgtag cgtggtcagc agactggaat cgccttcgag
     1141 ttgcgtttcg acacgcgcgt tcaactgcgt gttttcgaat tcgctgtgat cgcggtagcc
     1201 atcggtggac gtatccgaca ggttgaacag gtagttgacg cggccggtat cgccgccgag
     1261 cttgatttgc gcgttgcgaa aatcgtaatc gccgacggtg acccgcccgc tggcgaacgg
     1321 caccagcgaa cccttttccg attcgaccag gatcgcgccg cccgaggcat tgccgtacag
     1381 cgacgatgtc gggccgcgaa tcacggtgat ctcgcgcgcc gagccgagat cgatgccgtc
     1441 caccgagccc tggccgtcgg gcagagtttc cggtatgccg tcgacgatga tcttgatgcc
     1501 gcgaatgccg aaactcgagc gtgcgccgaa accgcgaatc gaggcgcgca ggtcctgcgc
     1561 aaaattgtag cggttctgca tgaacaggcc cggcactccg ccgagcgatt cgtcgagacc
     1621 gagttgctgg gtgccgagct gtatatcgtc ctggccgacg acgctgaccg ccgccgggat
     1681 ttcgtcgaga ttcttttcga cccgtgtcgc ggtgaccgtg atcggtccga gcccgctgtc
     1741 ctgggccgcg gcaaccgcgg acaacaggcc cgcggacagc aggattatgg cttgtagctt
     1801 cattaatatt ctccagtgcc ggcgcgaatc ccggccggcc tgccgatcga gccggctttg
     1861 tttaccaatc tctcggctgg ccgcgagtgc gaggttgtcg ataaaaacat tcttcaggct
     1921 aatcgaaatc gagcacttcg acacgtatcc ccgagtacca gtcggcgaca ttcgcgatgt
     1981 ccgcgtccgt tagcgacttc gcgatcggtg tcatctgcgg gtgctcgatc ttcctcgagc
     2041 ggtaagcctt caggcgctcg acgatgtagg gcctgggctg accgctgaga tcagggaccg
     2101 gcgacgaact gctgctgaca accgcctgcc cgtcgagacc gtggcagtgc tggcagagtt
     2161 gctcggcccg ggccttgccg gcgcgctcgc tctgctcgag agcctcgtcc gcgatgtcga
     2221 ggttacctga aagatcaatt tgcaggccgg cgtaccaggc ggcgacgttg tcgatgtcct
     2281 gctcggagat catttgtgcg acgaatgtca tctgcgcgtg ttcgcgcttg cccgaacggt
     2341 aatccagcag ccgcgagacc aggtattccc gattttgcgc tgtcaatttc ggcgacagcg
     2401 cggaattccc cccgctggcg gcctcgccat agagtccgtg gcaattggcg cagacctgct
     2461 caaccttgat gcggccatca tccacgtctt cgccggcaac cgcggccggt gccaggcaca
     2521 gcgtgaccac ggccatcagg cacggcgcaa gtttcagacg tgcaaggatt ctcgtcacgg
     2581 tctgcattgc aacaaaaccg acgggccgac
//
LOCUS       DATCZV010000328         3429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_449976, whole genome shotgun sequence.
ACCESSION   DATCZV010000328 DATCZV010000000
VERSION     DATCZV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449976"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VIW27_07380"
     CDS             <1..501
                     /locus_tag="VIW27_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY5739531.1"
                     /translation="ESSSKVIDENELILLDTDVVGCHGYYSDFSRTFHAGPKRPTRQQ
                     RELYRTAYEQVHHNMELIRPGISFREYSERAWNIPERYFANRYYLSAHGVGLTGEFPY
                     LYHRHDYAEAGYDGIIEPGMTICVESYIGEDGGSEGVKLEQQLQVTETGNRLLSKFPF
                     EPDLMY"
     gene            complement(547..2178)
                     /locus_tag="VIW27_07385"
     CDS             complement(547..2178)
                     /locus_tag="VIW27_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEY5739532.1"
                     /translation="MIETHAPSAYPYPLLIRHLWHTPLNNNPQQQIVSGESRRYDYTI
                     LRERIGRLAAALTELGIGKGNTVAVMDWDSHRYLECFFAIPMMGAVLHTVNVRLAPEQ
                     ILYTINHATDDAILLHADFMPIIEQIRDRIQRPPRLVYIDDREDAATPPGFAAEYESM
                     LAAVGTGHEFEDFDENTTATTFYTTGTTGEPKGVFYSHRQLVLHTLGLVADLGSADGM
                     HRFHRGDVYMPLTPMFHVHAWGMPYAATLMGVKQVYPGRYQPARLLQLIAREGVTFSH
                     CVPAILQMLLAAPAADAVDLSSWKVVIGGSAMPQGLARAAAERGIAAWTGYGLSETCP
                     LLTMADMSGITEVDTGVAGVARRCVTGKPVGMVELRVVDARMNEIPRGGAASGEIVVR
                     APWLTQGYAGDQPGSQALWESGYLHTGDVGYLDDGGSLHITDRLKDVIKSGGEWISSL
                     QLEDIVSRCDGVAEAAAIGVADHRWGERPLLIVVRAKPELSAEQVLASIRAEVDGGRL
                     SKWALPERVEFVDALPRTSVGKLDKKALRGLYGND"
     gene            2325..2714
                     /locus_tag="VIW27_07390"
     CDS             2325..2714
                     /locus_tag="VIW27_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5739533.1"
                     /translation="MKRFIIVASCACALAIAGSTLAADAVKIENLLRSELQVTEGVEV
                     IVSLVEIGPDFALPKHYHPGEEFVYVLEGSAIVWQQGKPDVTLNAGEIYRIPLKQVHT
                     AVTGNSSAKAIVFRVHEKGAPDRIPVE"
     gene            complement(2731..>3429)
                     /locus_tag="VIW27_07395"
     CDS             complement(2731..>3429)
                     /locus_tag="VIW27_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739534.1"
                     /translation="GLKPARPLVQLLRGLTMAGATTAFVIGVETVDYADAIAILYAYP
                     FVLVILAVLFLGERAGWTSWIGVVAGFAGVLLVMRPQFDNFNSGNLYIFGCAVIIGIQ
                     LTLNRKLSIVSPPLVTAFAGAVCASLSLTLMLPGSWAPIPDDAWWLIGLLVVSGGANQ
                     TMLVFAFAHADASTLAPFTYSEIVSAVIFGFLFFGTLPSALSWVGILLITASGIYVAH
                     ALHLQNIPRRVTKV"
BASE COUNT          642 a         1081 c         1122 g          584 t
ORIGIN      
        1 gaatcgtcga gcaaagtgat cgacgaaaac gaactgatcc tgctcgatac cgacgtcgtc
       61 ggctgtcatg gctattactc ggatttttcg cgcacgtttc atgccggccc gaagcggccg
      121 acgcgccaac agcgcgagct gtaccgcacc gcctacgagc aggtgcatca caacatggaa
      181 ttgatccggc ccgggatctc gttcagggaa tactccgagc gcgcgtggaa tattcccgag
      241 cggtacttcg ccaaccgcta ctacctgtcc gcgcacggcg tcggcctgac cggcgagttt
      301 ccgtaccttt accatcgcca cgattatgcc gaagccggct acgacggcat catcgagccg
      361 gggatgacga tctgcgtgga gagttacatc ggcgaagacg ggggttccga gggggtcaag
      421 ctggaacagc agttgcaggt caccgagacc ggcaaccggt tactgtcgaa gttcccgttc
      481 gaaccggatc tgatgtactg atcggggctc gaggagagcg ccggcaaccc acttgccggc
      541 cggggtctag tcgttcccgt acaggccgcg cagcgccttc ttgtcgagct tgccgacgct
      601 ggtgcgcggc agcgcatcga cgaattcgac ccgctcgggc agcgcccatt tcgacagccg
      661 gccgccgtcg acctcggcgc ggatcgacgc gagcacctgc tcggcgctca gttccggttt
      721 ggcgcgaacc acgatcagca gcggcctttc gccccaacgg tggtcggcga cgccgatggc
      781 cgccgcctcg gcgaccccgt cgcaacgcga aacgatatcc tcgagttgga gcgacgagat
      841 ccactcgccg ccggacttga tcacatcctt gaggcgatcg gtgatatgca gcgaaccacc
      901 gtcgtcgagg tagccaacgt cgccggtgtg caggtatccc gactcccaca gcgcttgcga
      961 accaggctgg tcgccggcat aaccctgcgt cagccatggc gcgcgcacga cgatttcacc
     1021 gctggccgcg ccgccgcgcg gaatctcgtt catgcgtgca tcgaccacgc gcagctcgac
     1081 catgccgacc ggcttgccgg tcacgcagcg ccgcgcgacg ccggcgacgc cggtgtcgac
     1141 ctcggtaatc cccgacatgt cggccatcgt cagtagcggg caggtttccg acaggccgta
     1201 tccggtccag gcggcgattc cccgttcggc cgcggcgcgc gccagcccct gcggcatcgc
     1261 cgagccgccg atcacgacct tccagctcga caggtcgacg gcgtcggccg cgggtgctgc
     1321 cagcagcatc tgcagtatcg ccggcacgca gtgtgaaaag gtgacgcctt cgcgggcgat
     1381 cagctgcagc agccgcgccg gttggtagcg ccccgggtag acctgcttga cgcccatcag
     1441 cgtcgccgcg tagggcatgc cccaggcgtg gacgtggaac atcggcgtga gcggcatgta
     1501 gacgtcgccg cgatgaaagc ggtgcatgcc gtcggcggaa cccaggtccg ccaccaggcc
     1561 gagagtatgc agcaccagtt gccggtggct gtagaaaacg cctttcggct cgccggtggt
     1621 gccggtcgtg taaaaggtcg tcgcggtggt attttcgtcg aagtcctcga actcgtggcc
     1681 ggtgccgact gccgccagca tcgattcgta ttccgcggcg aagcccggcg gagtggcggc
     1741 atcttcgcgg tcgtcgatgt acaccagccg cggcggtcgc tgtatacggt cgcggatctg
     1801 ctcgatgatc ggcatgaaat cggcatgcag caatatggcg tcgtcggtcg cgtggttgat
     1861 ggtgtagaga atctgctcgg gtgccaggcg cacgttgacg gtatgcagca ccgcgcccat
     1921 catcgggatc gcgaaaaagc actccaggta gcgatggctg tcccagtcca tcaccgcgac
     1981 cgtgttccct ttgccgatac ccagttcggt gagcgccgct gccagccgcc cgatgcgttc
     2041 gcgcaagatc gtgtaatcgt agcgccgcga ttcgccgctg acgatttgct gttgcgggtt
     2101 attgttgagc ggcgtgtgcc acagatgcct gatcagcagc gggtagggat aggccgaagg
     2161 cgcgtgcgtc tcgatcatga tcatcgatcc cgtcgtttgc ggctcacgat tatatcccag
     2221 ccgacggcgg ctcgggtaag ataggcggat tgcaggggcc gtcgtgaaca ataaccctgt
     2281 ccgcgacggc atgtttccca cattgaaaaa gcgggatcga aattatgaaa agattcatca
     2341 tcgtcgcgag ctgcgcctgc gcgttggcta ttgccggtag tactttggct gctgacgcgg
     2401 tcaagatcga aaacctgttg cgcagcgagc tgcaggttac cgaaggtgtc gaggtcatcg
     2461 tcagcctggt cgagatcggg cccgatttcg cgctgccgaa gcactaccac cccggcgagg
     2521 aattcgttta cgtgctcgag ggctcggcga tcgtctggca gcagggcaag cccgacgtga
     2581 ccttgaacgc cggggagatt tatcgtattc cactcaagca ggtgcacacc gcggtgaccg
     2641 gcaacagcag cgccaaggca atcgtgttcc gcgtccacga gaagggagca cccgaccgca
     2701 tcccggtgga gtgacccgcg gccggcgcgt tcagaccttg gttacgcggc gcggtatgtt
     2761 ttgcaggtgc agcgcatggg cgacgtagat gccgctggca gtaatcagca ggatgccgac
     2821 ccaggatagc gccgacggca gggtgccgaa gaacaggaag ccgaagatca ccgcggagac
     2881 gatctccgag taggtaaacg gcgccagcgt cgaggcgtcg gcgtgggcga aggcgaacac
     2941 cagcatggtc tggttggcgc cgccggacac caccagcagc ccgatcagcc accaggcgtc
     3001 gtcggggatc ggggcccagg atcccggcag catcagggtc aggctcagcg acgcgcagac
     3061 cgcgccggcg aaggcggtca ccagcggtgg cgagacgatc gacagcttgc ggttcagcgt
     3121 cagttgaata ccgatgatca ccgcgcagcc gaagatgtag agattgccgc tgttgaaatt
     3181 atcgaactgc gggcgcatca ccagcagcac gccggcaaag ccggcgacga cgccgatcca
     3241 cgaagtccag cccgcgcgct cgccgagaaa cagcacggcg agaatcacca gcacgaacgg
     3301 gtaggcatag aggatcgcga tcgcatcggc gtagtcgacg gtctcgacgc cgatcacgaa
     3361 ggccgtggtg gcgccggcca tcgtcaggcc gcgcagcaat tgcaccagcg gccgggccgg
     3421 tttcaggcc
//
LOCUS       DATCZV010000329         6483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_238158, whole genome shotgun sequence.
ACCESSION   DATCZV010000329 DATCZV010000000
VERSION     DATCZV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238158"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VIW27_07400"
     CDS             complement(<1..1014)
                     /locus_tag="VIW27_07400"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5739535.1"
                     /translation="MKGTDLPAGERGAFDPEARRSPTLPSRYYHDAEIHRRELQAIFH
                     RSWCYVGHASQLARPGDYRVEQVADQCILLLRDRESRLRAFFNVCQHRGHQLLSGVGS
                     GKKHIVCPYHAWSYGLDGQLVQAPHTEQLEGFEAADFALQPVRLAECAGLLFANLDAA
                     ATDFEREYPGLEETITASLPDHGQLVAAHQFDFDIAANWKIVVDNFSEGYHIPVAHRL
                     LSQVLDNTTGSCRRRESRYAFFESRSKAGYDGDRIDAGSPYRSWTIWPNTCMLSLPGC
                     ENLVVIRMAADGPGRCREHADLLAAPNAPAEPLQAVRKLFAEQFNAEDIAIVESVHRG
                     LQSL"
     gene            1152..2105
                     /locus_tag="VIW27_07405"
     CDS             1152..2105
                     /locus_tag="VIW27_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5739536.1"
                     /translation="MPSQHTPDFMDHLRETEFMLMDGALGTELERRGVPFEGSGWSAL
                     AVRDHPDTVRRVHQDYIRAGADLHIVNSFALAHHVLEPLGLGDQFEDLNQRAVALFDD
                     AAVRCGVERRRLWAAGSLSTFAPHSNRGRLPQGAALIANYRRQAEILEAAGVDLLALE
                     MLFDIETSLAMLTALRDSSLPVVLGFCCDRDRQDGTGITTKLGMGGPVQPFADILPRV
                     LDAADPQRSIAAIMHSDFAVTDAALEILRDHWDGALAVYPNSGYFVDLQMQFDSVCGV
                     GEFTAAACRWIDAGVQIVGGCCGIGPAHIEGLRAATGIESR"
     gene            complement(2285..2818)
                     /locus_tag="VIW27_07410"
     CDS             complement(2285..2818)
                     /locus_tag="VIW27_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEY5739537.1"
                     /translation="MLRISLIGIVLLSMAGCASGPPEPISRAVHDNPSLTRVRLDIEA
                     FVGTDVRWGGMVSKVENKADRTWIEIVKMPLRSNGKPESAGSSEGRFIASFDRFLDPV
                     VYELGSRLTVVGRIDGSVRRPIGDHDYLFPIVAVDGSYLWQPLDHLPPPAYPPPWWYY
                     DPWYPYPWPYYRHPRYY"
     gene            3003..3644
                     /locus_tag="VIW27_07415"
     CDS             3003..3644
                     /locus_tag="VIW27_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY5739538.1"
                     /translation="MKFERIFCFGDSITLGCNDSQGLGWPGRLCRGLHCGDYSVATYN
                     LGVNGDTSAHIAARWRAEAEARSRNSPGLLIFAFGFNDAAHADGSGPQLELAESVATA
                     RELLSAAQQVSSLLWIGPTPLDEAVNPMPTPVASWEMHNAEIARYDAAYSRLAAELGI
                     DYLPLFAEFVDSPRYRAALAAGDRVHPGDDGYAMIAERIAAWDSWIEILRAPG"
     gene            complement(3641..>6483)
                     /locus_tag="VIW27_07420"
     CDS             complement(3641..>6483)
                     /locus_tag="VIW27_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5739539.1"
                     /translation="RTLGAEIEMLEQELLSQPMRTELTGAQRDLATLRMSRQKQFVDL
                     LEGLVVERRRSEAETVQQAAEETERQAFGKHPLLQQLAQRNTELGDELKQLTVDLEAI
                     TRDENLAASEARRVSDNYRMARQKLEIAGLSEALGQALLEQRRGLPDYKDFRAAEQRR
                     EQMVVEASLRQIRHQQERGRLRDLDAYVDELLASVPQTWQVLLRDEIRELASLRRELI
                     DKAVAADDTYLQALGELDLAQRQLAEAVNAYNGYLAERLLWVRTGEPPSWQMLRSIGS
                     RLAIFFDPDHWFDLLQSLAFPQRFPGLLLLGLVLFALLLRWRGRFRNSLEQSGRNVGQ
                     LRHDRYLATMRALLLTLLLAAPWPLLFIALGAHLQLSQQSVLVALDIELSQTAGWSGQ
                     FVPAIGAALYKNALYLFYFIAFRIFCEPSGLAVTHFGWSPDVAGQLRRETRRLMAVFL
                     PSTFILIAAVSYDPAALAGGMSRLFFVIVLLSLSWFFSHVMAPRSGVLRRYYSDHPGS
                     PLTWFRYVSMTLGLLLPISLALLVIFGYVYTAAQFGTRLLDTLWLIVAIILVHQLLVR
                     WVLLTERQLVFKDALERHRAQRAAREADQEADTGAAENALLAAEETSVDYVALSANSK
                     KLINTALTVIAAVGMWLIWSDVLPAFRILNEISLWSYASAVDDVERLIPVTLGDAIVA
                     LLIVFFGWIAALQLPSLIEIVLLSRLEITAGSRYAISKLTQYSIVATGIVMVFGILGG
                     SWDEIQWLVAALGVGIGFGLQEIVANFICGLILLFERPIRIGDVVTVGDTSGVVTKIR
                     IRSTTIRNWDQKELLVPNKEFITGRLLNWTLSDQVARIVVSVGIAYGSDVQLALKILR
                     EVADENERVMSDPEPLVIFEQFGDSALLLVLRCYIDSIDYRLQTQSELNLAIDRKFGE
                     AGIVIAFPQRDLHLDTAKPLEVRILEPQPEAG"
BASE COUNT         1206 a         2122 c         2044 g         1111 t
ORIGIN      
        1 cagcgattgc aggccgcgat gcacactctc gacgatcgcg atatcctcgg cgttgaactg
       61 ctcggcgaac agcttgcgca cggcttgcag cggctctgcc ggcgcgttcg gcgccgccag
      121 caggtcggca tgttcgcggc agcgccccgg accgtcggct gccatgcgga ttaccaccag
      181 gttctcgcac cccggcaggc tcagcatgca ggtgttcggc catatcgtcc acgagcgata
      241 cggcgacccg gcatcgatcc ggtcaccgtc gtatcccgcc ttcgagcgcg attcgaagaa
      301 tgcgtagcgg gactcgcgcc gccggcagct gccggtggta ttgtcgagca cctgtgacag
      361 caaccgatgg gcaaccggaa tgtggtaacc ctcggaaaag ttgtcgacga cgatcttcca
      421 gttggcggcg atatcgaaat cgaactggtg cgcggcgacc agctggccgt ggtccggcag
      481 gctcgcggta atcgtttcct cgagacccgg gtactcgcgt tcgaaatcgg tggccgcggc
      541 gtcgagattg gcgaacagca gtcccgcgca ctccgccagc ctgaccggtt gcaacgcgaa
      601 atccgccgcc tcgaaaccct cgagttgctc ggtatgcggt gcctgcacga gctgcccgtc
      661 gagcccgtaa gaccaggcgt gataggggca gacgatatgc ttcttgccgc tgccgacacc
      721 gctcaacagc tggtggccgc gatgctggca aacgttgaag aaagcgcgca gccgcgattc
      781 gcggtcgcgc aacaacagga tgcactggtc ggcgacctgc tcgacccggt agtcgccagg
      841 ccgcgccagc tggctggcat gtccgacgta acaccagctg cggtgaaaaa tagcctgtaa
      901 ttcgcggcgg tggatctccg cgtcgtggta ataacgcgac ggcagggtcg gcgagcggcg
      961 cgcctcggga tcgaacgcgc cgcgctcgcc ggccggtaag tcggtgccct tcattgcctg
     1021 gtcaagctcc cgtggtttgc agataatcca tcaaatcacg acatgggcgg agtgtccagc
     1081 atgccggcga ttccacccgc cgtggccagc ggttacactg gccgaccacc tcttgtcgcg
     1141 gagcagcgcc gatgcccagt caacacacgc cggatttcat ggatcacctg cgggaaaccg
     1201 aattcatgct catggacggc gccctgggta ccgagctcga acggcgcggc gtgccgttcg
     1261 aaggcagcgg ctggagcgcg ctggcggttc gtgatcaccc cgataccgtg cgtcgcgtcc
     1321 accaggacta catacgcgca ggcgccgatc tgcatatcgt caacagtttc gcgctcgcgc
     1381 accacgtgct cgagccgctg ggtctcggcg accagttcga ggatctgaac cagcgcgccg
     1441 tggccttgtt cgacgacgcc gccgtccgct gcggcgtcga acgccgcagg ttgtgggcag
     1501 ccggctcgct gtcgaccttc gccccccatt cgaatcgcgg acgcctgccg caaggggctg
     1561 cgctgatcgc caattaccgc cggcaggccg agatcctgga agcggccggg gtcgatctgt
     1621 tggcgctgga aatgctgttc gatatcgaga ccagcctggc gatgctgacc gcgctgcgcg
     1681 acagcagctt acccgtggtt ctcggttttt gctgcgaccg cgaccggcag gacggcaccg
     1741 gcatcaccac caagctgggt atgggcggcc cggtacagcc atttgccgac atcctgccca
     1801 gggtactcga tgccgccgat ccgcaacgca gcatcgccgc gatcatgcac agcgatttcg
     1861 cggtcaccga tgcggccctg gaaatccttc gcgatcactg ggacggcgca ctcgcggtat
     1921 atccgaactc gggatatttc gtcgacctgc agatgcagtt cgacagcgtc tgcggggtcg
     1981 gcgaattcac cgccgcggca tgccgctgga tcgacgccgg cgtacagatc gtcggcggct
     2041 gctgcggtat cgggcccgcg catatcgagg gcctgcgcgc cgcgaccggc atcgagtctc
     2101 gctgatcttc caggaacggt cgacgcgggc agcgtcaacg gcgagcaaac cagtcccgcc
     2161 gtcgaactcg gcgcacaggg caacctgcat ttgctgtgga tcgaacgcgc cgggccgaac
     2221 gcgccgagcc ggcggtggta cagtcacggc ctgccgcgcc ggccgttaac cacgccaacg
     2281 cgcgtcagta gtagcgcgga tgccggtagt acggccaggg atagggatac cagggatcgt
     2341 aataccacca cggtggcgga taggcgggcg gcggcaagtg atccaacggc tgccacaggt
     2401 aagagccgtc gacggcgacg atcgggaaca ggtaatcgtg atcgccgatg ggccgccgca
     2461 cactgccgtc gatgcgcccg accacggtca gccggctgcc gagttcgtag accaccgggt
     2521 cgaggaaacg gtcgaagctg gcgatgaagc ggccctcgct gctgccggca gactcgggtt
     2581 tgccgttgct gcgcaacggc atcttgacga tttcgatcca ggtccggtcg gccttgtttt
     2641 cgaccttgct gaccatgccg ccccagcgga cgtcggtacc gacgaaggct tcgatgtcga
     2701 gacgcacgcg ggtcaacgac ggattgtcgt gcaccgcgcg gctgatcggt tcgggcggtc
     2761 cgctggcaca gcccgccatc gataacaaaa caatgccgat tagcgatatt ctgagcatag
     2821 cctttactac ctgccgactc gtcgaattca atgcggcccg gcctgataat ttatcatcgg
     2881 ccgccgccgc gcgatagccc cgcgtgtcgc cgtcaatcca tttttcgggg aattccgggc
     2941 ggcactgttg ttcgtcgccc gctcctttta caatccctaa cgacttggac aggcgaaagg
     3001 ccatgaaatt cgaacgtatt ttttgcttcg gcgacagcat cacgctcggc tgcaacgaca
     3061 gccagggtct cggctggccc ggccgcctgt gccgcgggct gcactgcggt gattacagcg
     3121 tggccaccta caatctcggg gtcaacggcg acacctcggc gcatatcgcc gcacgctggc
     3181 gcgccgaagc cgaggcgcgc agccgcaatt caccgggcct gctgatcttc gctttcggtt
     3241 tcaacgatgc cgcgcatgcc gacggcagcg gcccgcagct cgaactggcg gagtcggtgg
     3301 caaccgcacg cgaattgctg agcgccgcgc aacaagtctc gagcctgctg tggatcggtc
     3361 cgacgccact cgacgaagcg gtcaacccga tgccgacgcc ggtcgccagc tgggagatgc
     3421 acaacgccga aatcgcgcgc tacgatgccg cctattctcg gctcgcggcc gagctgggca
     3481 tcgattacct gccgttgttc gcggagtttg tcgacagccc gcgctaccga gcggcgctcg
     3541 ctgccggcga ccgggtacac ccgggcgacg acggctacgc aatgatcgcc gagcgcatcg
     3601 ccgcctggga cagctggatc gagatcctgc gcgcgccagg ctagccggcc tcgggctgcg
     3661 gctcgagaat gcgcacctcg agcggcttcg cggtatccag gtggagatcg cgctgcggaa
     3721 aggcgatcac gatacccgcc tcgccgaact tgcggtcgat ggccaggttc agctcgctct
     3781 gcgtctgcag ccgataatca atcgaatcga tgtagcagcg cagcaccagg agcagcgcac
     3841 tgtcgccaaa ttgctcgaag atcaccagcg gctccggatc actcattacg cgctcgtttt
     3901 cgtcggccac ctcacgcaat attttgagcg ccagctggac atcgctgcca taggcaatgc
     3961 cgacactaac cacgatacgc gcgacctggt ccgaaagcgt ccagttcagc aggcggccgg
     4021 taatgaattc cttgttcggc accagcaatt ccttctggtc ccagttgcga atcgtcgtcg
     4081 agcggatgcg gatcttggtc accacgccgg aagtgtcgcc gaccgtgacc acgtcgccga
     4141 tgcgaatcgg gcgctcgaac aacaggatca acccgcagat gaaattggcg acgatttcct
     4201 gcaggccgaa gccgataccg acaccgagcg ccgcaaccag ccactggatt tcatcccagc
     4261 tgccgccgag tatgccgaac accatcacga tgcccgtcgc gacgatgctg tactgggtca
     4321 gcttggagat cgcatagcgg ctgccggcgg tgatttcgag gcgcgacagc agcacgatct
     4381 cgatcagcga cggcagctgg agtgccgcaa tccagccgaa gaaaacgatc agcagggcga
     4441 cgatcgcgtc gcccagcgtg accggtatca gccgctccac gtcgtccacg gcgctcgcgt
     4501 agctccacaa ggatatctcg ttgagaatgc gaaacgcggg cagcacatcg gaccagatca
     4561 gccacatacc gaccgcggcg atcaccgtca acgcggtatt gatcagcttt ttcgagttcg
     4621 cgctcagcgc gacgtaatcg acactggttt cctcggcggc cagcagcgcg ttctcggcgg
     4681 caccggtatc ggcttcctga tccgcttcgc gcgccgcgcg ctgcgcgcgg tggcgctcca
     4741 gcgcatcctt gaacaccagc tggcgctcgg tcagcagcac ccagcgcacc agcaactgat
     4801 gaaccaggat gatcgccacg atcagccaca gggtatccag cagccgggtg ccgaactgcg
     4861 ccgcggtata gacgtagccg aaaatcacca gcagcgcgag cgatatcggc aacagcaatc
     4921 ccagagtcat cgagacatag cgaaaccagg tcagcggact gcccggatga tcgctgtaat
     4981 agcggcgcaa taccccgctg cgcggtgcca tgacgtggct gaagaaccag ctgagcgaca
     5041 gcagcacgat gacgaagaac aggcggctca tgccccccgc cagtgccgcc ggatcgtagc
     5101 tgacggcggc tatcaggatg aaagtcgacg gcaggaacac cgccatcagg cggcgggttt
     5161 cgcggcgcaa ttgtccggcg acgtccgggc tccagccgaa gtgagtcacc gccagcccgc
     5221 tgggctcgca gaaaatccga aaggcgatga aataaaacag gtagagcgcg tttttatata
     5281 gtgccgcgcc gatcgccggc acgaactggc cgctccaacc ggctgtctgg ctgagttcga
     5341 tatccagcgc gaccagcacg ctttgctgcg acagctgcag gtgcgcgccg agcgcgatga
     5401 acaacaacgg ccagggtgcg gccagcagca gcgtcagcag cagtgcgcgc atcgtggcca
     5461 ggtaccgatc gtggcgcagc tggccgacgt tacggccgct ttgttcgagc gaattgcgaa
     5521 aacggccgcg ccatctgagc aataacgcga ataataccag gccgagcagc aatagccccg
     5581 ggaaccgctg cgggaaggcc agcgactgga gcaagtcgaa ccagtgatcg ggatcgaaaa
     5641 aaatcgccag ccggctaccg atggagcgca gcatctgcca cgatggcggc tcgccggtac
     5701 gcacccacag caggcgttcg gcgaggtagc cgttgtaggc gttgaccgcc tcggccaact
     5761 gtcgctgggc caggtccagc tcaccgagtg cctgcaggta ggtatcgtcg gctgcgaccg
     5821 ctttgtcgat caattcgcgg cgcaaactgg cgagttcccg gatttcgtcg cgcagcagga
     5881 cttgccaggt ctgcggcacc gacgccagca gctcgtcgac ataggcgtcc aggtcgcgca
     5941 ggcggccacg ttcctgctga tggcgaatct ggcgtaagct cgcctcgacg accatttgct
     6001 cgcggcgctg ctcggcagcc ctgaaatcct tgtaatccgg caggccgcgg cgttgctcga
     6061 gcagggcctg gccgagggct tcgctcaagc ccgcgatctc cagcttctgc cgcgccattc
     6121 ggtaattatc gctaacccgc ctggcctcgc tggcggcgag attttcgtcc cgggtaatgg
     6181 cttccaggtc gaccgtcagc tgcttcagtt catcgccgag ctcggtgttg cgctgcgcca
     6241 gctgctgcag cagcgggtgc ttgccgaaag cctggcgctc ggtttcctcg gcagcctgct
     6301 ggacggtctc ggcctcgctg cgacggcgct cgacaaccag cccttcgagc agatcgacga
     6361 actgtttctg gcggctcatg cgcagcgtcg ccagatcacg ctgcgcgccg gtcaactcgg
     6421 tgcgcatcgg ctggctgagc agttcctgct cgagcatttc gatctcggca cccagcgtac
     6481 gat
//
LOCUS       DATCZV010000330         5179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_768038, whole genome shotgun sequence.
ACCESSION   DATCZV010000330 DATCZV010000000
VERSION     DATCZV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768038"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIW27_07425"
     CDS             <1..237
                     /locus_tag="VIW27_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5739540.1"
                     /translation="NLDRDERDLALCEAVIAMAHKLGIEVIAEGVENETQQSLLVQFG
                     CDYAQGHLYSRPLPAAEFELLLERQAKRDRPLDG"
     gene            complement(473..1351)
                     /locus_tag="VIW27_07430"
     CDS             complement(473..1351)
                     /locus_tag="VIW27_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY5739541.1"
                     /translation="MSSSTSNEEYYVPHGTHWPIIGSIGLTLTVVGVANYLHGTWSLT
                     PVFVGLAILFFMMYGWFGQVVSESLRGYYNQQVDATFRWSMSWFIFSEVMFFAAFFGA
                     LYYARQFSIPWLDGASNNDATAQYLWPDFEAAWPLLAVPLADKYEVAREAIGPWGLPA
                     LNTIILLTSGLTVTLAHWALKRRDNVWLVYWLFATVALGFGFVVLQAWEYAHLYQDLN
                     LRLDSGIYGSTFFMLTGFHGFHVTMGATMLLVIMIRAAKGHFTPENHFAFEGVAWYWH
                     FVDVVWLGLFIFVYWL"
     gene            complement(1390..1902)
                     /locus_tag="VIW27_07435"
     CDS             complement(1390..1902)
                     /locus_tag="VIW27_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017821600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5739542.1"
                     /translation="MKTSPLKLAMIPVLMFGFGFALVPLYDVFCDITGLNGKTGRIEA
                     AEIDSTAIDTSRTIEVSFLANTSNGLPWSFEPLVSTMEVHPGQVYEAVYRVRSSSGEK
                     TLGQAIPSVSPGLAAQHFNKTECFCFSQQALEGYETRDMPLRFIVGNDIPEKIDAITL
                     SYTFFSQDQG"
     gene            complement(1899..2036)
                     /locus_tag="VIW27_07440"
     CDS             complement(1899..2036)
                     /locus_tag="VIW27_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739543.1"
                     /translation="MQQDHQEQDSLTSVERRKRTWRIVIAHLLIVTTFFIASFLWGSF
                     K"
     gene            complement(2061..3665)
                     /gene="ctaD"
                     /locus_tag="VIW27_07445"
     CDS             complement(2061..3665)
                     /gene="ctaD"
                     /locus_tag="VIW27_07445"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY5739544.1"
                     /translation="MQTETTHDDVHGAHDEHDHHGPAKGLMRWVTTTNHKDIGTLYLW
                     LSFIMLLYGGSMAMVIRLELFQPGLQWVDPHFFNQMTTMHGLVMVFGAIMPAFVGLAN
                     WLVPMMIGAPDMALPRLNNWSFWILPFAFTMLASTTFMEGGAPAFGWTFYAPLSTTFA
                     PDSTDFFIFAVHLMGISSVMGSINIIATILNMRAPGMTLMKMPLFVWTWLITAYLLIA
                     VMPVLAGAVTMMLTDRHFGTSFFDAAGGGDPVLFQHVFWFFGHPEVYIMILPAFGIIS
                     AIVPTFARKPLFGYSSMVYATASIAILSFMVWAHHMFTTGMPVAGELYFMYATILISV
                     PTGVKVFNWVSTMWKGSMTFETPMLFALGFIIMFTIGGFSGLMLGVAPVDTQYHDTYF
                     VVAHFHYVLVTGALYSIIAAFYYWVPKWTGHMYDETLGKIHFWWSTIAVNVLFFPQHF
                     VGLAGMPRRIPDYSLQFAEFNAISSIGGFAFGLAQVFFLYIVIKTIKGGKKATDQVWE
                     GATGLEFEQLSSPPPYHSFTQAPPVR"
     gene            complement(3683..4816)
                     /gene="coxB"
                     /locus_tag="VIW27_07450"
     CDS             complement(3683..4816)
                     /gene="coxB"
                     /locus_tag="VIW27_07450"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5739545.1"
                     /translation="MILKKLSRPGALIPVGLGLLLASSVARAEYALNMTESVTPLGRE
                     LYSLHMLVFWICVVIGVVVFGAMAWSIIYHRKSQGAEAANFHESTTIEIVWTVVPLLI
                     LIGIAIPATGTLIDLEDAKTDADITLQVTGIQWKWKYTYIDEDVSFVSSLAQSSRDVV
                     SDPTGNENYLLEVDKPVVLPINKKVRFLFHSDDVIHAWWVPDLAVKQDSIPGFINDSW
                     AIIEEPGTYRGQCAELCGKDHGFMPIVVEAVTEDEYQQWLVSQRAAAAEAAAAAEQEW
                     SVQDLLARGETVYQSNCAACHGPTGAGIPGAFPAMTGSAIVNGDPAAHIDIVVNGKAG
                     TAMAAYKSQLNDVDIAAVITYERNALGNSVGDTVQPSAIKNAR"
     gene            complement(4939..>5179)
                     /locus_tag="VIW27_07455"
     CDS             complement(4939..>5179)
                     /locus_tag="VIW27_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY5739546.1"
                     /translation="VITLDRRHTRIEKGVQRVDQRWQFDTPWTRLIDRQTGVSGGGRT
                     LALGSHGNFVEVGDFLDNSEKDRLAFTLKDCIIRG"
BASE COUNT         1144 a         1515 c         1560 g          960 t
ORIGIN      
        1 aacctggacc gcgacgagcg cgacctggcg ctgtgcgagg ccgtcatcgc aatggcccac
       61 aagctcggta tcgaggtgat agccgagggc gtggagaacg agacccagca aagcctgttg
      121 gtacagttcg gctgcgatta cgcccagggg cacctgtatt cgaggccgct tccggccgcc
      181 gaattcgagc tgctactcga gcgacaggcg aagcgagacc gccccctgga cggctgacct
      241 ccggacggtt agcccgaaat gaaaaaagcc cggcgatgcc gggctttttc gtcaaccagc
      301 gcgcagctat tgcgccgcgc agcaggggtt tttattgata taggcgaggc ctttggccgt
      361 cgcgagcggt gcaagagcaa ggcatttttt ccggcgatac cgcgaacagt acaaaccggt
      421 acgtgagcgg ttcgccggaa aaaataacgc agctattgcg ccgcgcagca ggtcaaagcc
      481 agtagacgaa gatgaacagg ccgagccaga ccacatccac gaagtgccag taccaggcga
      541 cgccctcgaa agcaaagtga ttctcggggg tgaaatgtcc cttggcggca cggatcatga
      601 tcaccagcaa catggtcgcg cccatggtca cgtgaaagcc gtggaaaccg gtcaacatga
      661 agaacgtact gccatagata ccggaatcga gacgcaggtt gagatcctgg tacaggtgtg
      721 cgtactccca ggcctgcagc accacgaagc cgaagcccag cgcgacggtc gcgaacagcc
      781 agtaaaccag ccagacatta tcccggcgct tcagcgccca gtgcgcaagc gttaccgtga
      841 ggccggaggt caacaggatg atggtattca gagccggcag gccccaggga ccgatcgctt
      901 ccctggcgac ttcgtacttg tccgcgagcg gcaccgccag cagcggccag gcggcctcga
      961 aatcaggcca caggtactgc gcggtggcgt cgttgttcga agccccgtcg agccacggaa
     1021 tcgagaactg gcgagcataa tacagggcac cgaaaaatgc cgcgaagaac atgacctcgg
     1081 aaaagatgaa ccagctcatg ctccagcgaa aagtcgcgtc gacctgctgg ttgtaataac
     1141 cacgcagcga ttcgctgacc acctggccga accagccgta catcatgaaa aacaatatcg
     1201 ccaggccgac gaataccggc gtcaggctcc aggtaccgtg caggtaattg gcgacaccga
     1261 ccacggtaag cgtgagcccg atcgagccga ttatcggcca gtgggtgcca tgtggtacgt
     1321 aatattcttc gttgctagta ctgctgctca tggatggtct ctctgtttat tcgaatactg
     1381 tttccatgcc tagccctggt cctggctgaa aaaagtatag gacagggtta ttgcatcgat
     1441 tttttccggg atatcgttgc cgacaatgaa tcgcaacggc atatcgcgcg tctcgtagcc
     1501 ctcgagcgcc tgctgtgaga agcagaagca ttcggtcttg ttgaagtgct gcgcggccag
     1561 ccccggcgaa acgctgggaa tcgcctgccc cagcgtcttc tcgccactgc tgctacgaac
     1621 ccggtacacg gcctcgtaga cctgtcccgg gtgcacttcc atagtcgaca ccagcggctc
     1681 gaagctccac ggcaggccgt tggacgtatt ggcgaggaaa ctgacctcga tggtacgcga
     1741 ggtatcgatc gcggtcgaat cgatctccgc cgcctcgata cgcccggttt tgccgttcaa
     1801 gccggtgatg tcacaaaaca cgtcgtacag cggcaccagc gcgaagccga agccgaacat
     1861 cagcaccggg atcatcgcca gtttcagcgg gctggtcttc atttaaagct gccccacaga
     1921 aacgaggcga tgaaaaaggt cgtgacgatc agcaggtgcg cgatcacgat ccgccaggta
     1981 cgcttgcggc gctcgacgga agtgagggag tcctgctcct gatgatcctg ctgcatcgaa
     2041 ccgattcatc gctgcttggc ttaacggacc ggcggcgcct gggtaaagct gtggtacggc
     2101 ggcggcgacg acaattgctc gaattccagc ccggtcgcgc cttcccagac ctggtcggtg
     2161 gctttcttgc cgcccttgat ggtcttgatg acgatataca ggaagaatac ctgcgcgagg
     2221 ccaaaagcga agccaccgat actcgaaatc gcgttgaact cggcgaattg cagcgagtaa
     2281 tccggaatcc gccgcggcat accggccagg ccgacgaaat gctgcgggaa gaacagcacg
     2341 ttgacggcga tggtcgacca ccagaagtgg atcttgccga gcgtttcgtc gtacatgtgc
     2401 ccggtccact tgggtaccca gtagtagaaa gcggcgatga tcgaatagag cgcgccggtt
     2461 accaatacgt agtgaaagtg cgcgaccacg aagtaagtat catggtactg cgtatcgacc
     2521 ggtgcgacgc cgagcatcag gcccgagaag ccgccgatcg tgaacatgat gatgaaaccg
     2581 agcgcgaaca gcattggggt ctcgaaggtc atcgaaccct tccacatggt gctgacccag
     2641 ttgaatacct tgacgccggt aggcaccgag atcaggatcg tcgcgtacat gaagtagagc
     2701 tcgccggcga ccggcatgcc ggtggtgaac atgtggtgcg cccagaccat gaacgacagg
     2761 atcgcgatcg acgcggtcgc gtataccatc gagctgtagc cgaacagcgg cttgcgcgcg
     2821 aaggtcggca cgatcgccga gatgatgccg aaggccggca ggatcatgat atagacctcg
     2881 ggatggccga agaaccagaa cacgtgctgg aacagcaccg ggtcgccgcc gcccgcggca
     2941 tcgaagaacg aggtgccgaa gtgacggtcg gtcagcatca tggtgaccgc gccggcgagt
     3001 accggcatca ccgcgatcag caggtaggcg gtaatcagcc aggtccagac gaacagcggc
     3061 atcttcatca gcgtcatgcc gggggcgcgc atgttgagaa tcgtggcgat gatgttgatc
     3121 gaacccatca ccgacgaaat acccatcagg tgcaccgcga agatgaagaa gtcggtacta
     3181 tccggggcga aggtagtcga caggggcgcg tagaaggtcc agccgaaagc cggtgcgccg
     3241 ccttccatga aagtcgtcga cgccagcatc gtgaacgcga acggcagaat ccagaaactc
     3301 cagttattca gccgcggcag cgccatgtcg ggggcaccga tcatcatcgg caccagccag
     3361 ttggcgagac cgacgaaggc cggcatgatc gcaccgaata ccatcaccag gccgtgcatg
     3421 gtggtcatct ggttgaagaa gtgcggatcg acccattgca gccccggttg gaacagttcg
     3481 aggcgaatca ccatcgccat cgagccgccg tagagcagca tgataaaaga cagccacaga
     3541 tagagcgtgc cgatatcctt gtggttggtc gtcgtcaccc agcgcatcag acccttggca
     3601 ggaccgtgat gatcgtgctc gtcgtgggcg ccgtgaacgt cgtcgtgtgt agtttcagtt
     3661 tgcatcggaa tggattcctc ttttatcttg cattcttgat cgcggacggc tgtaccgtgt
     3721 cgccgacgct gttacccagc gcgttgcgct cgtaggtaat taccgcggcg atgtcgacat
     3781 cattgagctg cgacttgtaa gccgccatcg cggtaccggc cttgccgttg acgacgatat
     3841 cgatgtgcgc cgcgggatct ccattgacga tcgcgctgcc ggtcatcgcc gggaaagcgc
     3901 ccggaatacc ggcacccgtg ggaccgtggc aggcagcgca gttggactgg tatacggttt
     3961 cgccgcgggc cagcagatcc tgcaccgacc actcctgctc ggcggccgcg gcggcctcgg
     4021 cggcggcggc tctctggctc accagccact gctggtattc gtcttcggtg actgcctcga
     4081 cgacgatcgg catgaagccg tggtccttgc cgcaaagttc tgcgcactgg ccgcggtagg
     4141 tgccgggctc ctcgatgatc gcccaggaat cgttgataaa gccgggaata gagtcctgct
     4201 tgaccgcgag atccgggacc caccaggcgt ggatgacgtc gtcggaatgg aacaggaaac
     4261 gaaccttctt gttgatcggc agtaccaccg gtttatccac ttccagcagg tagttttcgt
     4321 tgccggtagg atcgctgacc acgtcacggc tcgactgcgc caggctgctg acgaagctga
     4381 cgtcctcgtc gatgtaagtg tatttccatt tccactggat gccggtgacc tgcagggtga
     4441 tatcggcgtc ggtcttggcg tcctcgaggt cgatcagcgt gccggtagcg ggaatcgcga
     4501 tgccgatcag gatcagcagc ggcacgactg tccagacgat ctcgatggta gtgctttcgt
     4561 ggaagttggc ggcctcggcg ccctgcgact tgcggtggta aatgatcgac cacgccatgg
     4621 caccgaagac gaccacgccg atgacgacac agatccagaa caccagcata tgcaggctgt
     4681 acagttcgcg gcctagcgga gttacgctct cggtcatgtt caaggcatat tccgcgcgtg
     4741 cgacggacga cgccagcaac aatccgagtc cgaccggaat cagcgcaccg ggcctggaca
     4801 acttcttcaa tatcattctc gactcctaaa catagctccg cgacccggct tcccggtcgc
     4861 agtaaaaatg ccggcattta aacacaaaaa tgccacctga aaagtgcttt ttcgcttggc
     4921 ttcagacggc ggtggaaatc aaccgcgaat tatacagtct ttcaatgtaa acgctagacg
     4981 gtctttctcc gaattatcca gaaaatcgcc gacctccacg aagttgccgt gagatcccag
     5041 cgccagggtt ctgccaccgc cggaaacccc cgtctggcgg tcgatcaggc gcgtccaggg
     5101 agtgtcgaac tgccacctct gatcgactcg ctgcacgcct ttctcgatcc gcgtatggcg
     5161 ccggtcgagg gtgatgact
//
LOCUS       DATCZV010000331         5055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_662016, whole genome shotgun sequence.
ACCESSION   DATCZV010000331 DATCZV010000000
VERSION     DATCZV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662016"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VIW27_07460"
     CDS             <1..997
                     /locus_tag="VIW27_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY5739547.1"
                     /translation="APIRQIHVSARGHWGVTRLQASDYQLDALGYVVEFESLNRLLLE
                     AVDACAEITLEDGASFTSLSQDDGVRIEYRRQKRQRHLDARLALIADGADSAARAALG
                     IDHRRIDYGQSALVVNVEVGRPRDGLAYERFTSEGPLAMLPLGGKRYACVWTCSPARA
                     AELEAVDDAAFGAALQDCFGYRLGIVERVGKRVAVALMRTQAEALSRGRCVLIGNAAN
                     ALHPVAGQSFNLSLRDIAGLRELLVDHELAAIDDAAWQQIAAVYAESRAAEQRRVISY
                     GDGLVTLFSNPLPVFDRLRAAGLAALDLLPALKAQAAFSGMGLAFGGNRLLRGRR"
     gene            994..2178
                     /locus_tag="VIW27_07465"
     CDS             994..2178
                     /locus_tag="VIW27_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEY5739548.1"
                     /translation="MSALETDIAIAGAGMVGSCAAHALAANGLRVALIEAQPPPADPP
                     PADAAYDLRVSAISPRSRAILEHLGVWQQLDAARLCDYQKMHIWHQHGNASVGFDAVE
                     LAREYLGTIVENRQLQQALYRVCERQSTIEWFTPDRIAAIETNHGDGVELRLESGARI
                     AAQLLIAADGRNSPTRELVGIDAQAGRYRQTAIVANVDTEKAHRETAWQRFLATGPLA
                     FLPLANGQSSIVWSCDDDAVEALLAADEAAFCEALGDAFEYRLGRVVHAGERASFPLG
                     WHYCERWLAGRVLLIGDAAHGVHPLAGQGVNLGFSDVELLARLLRSRADLGSRSLLRR
                     YERRRKSETRVATASFSALKWIYGNQQILVSGARDLGMRAVEATPWLKRSLMRRAIEN
                     LT"
     gene            2305..3915
                     /locus_tag="VIW27_07470"
     CDS             2305..3915
                     /locus_tag="VIW27_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY5739549.1"
                     /translation="MRNGAGIFVVLTLVCMLPLRVAGQDTLEAVRFNVERFEVIGDNP
                     IGKRAVSVLAPFTGEQYGLEGLSAARDALERAIIDAGYTFHRVSLPPQDLFEGAVILR
                     VARFAIGRVSVEGNLYFDDANIRHSVPGLREGATPNTRRLSRSLKLANQHASKSTLLR
                     FAEGTGGDTIDAVLEVEDRDPQLFFVSLDNTGPEDGEIWRTTLGYQHGNLFNRDHSLT
                     ATLTTAPEDPEVTTQFGVNYHLPLYAHGASIDLLFSDADSAGETGGGGDTGQGIAPGT
                     GGGQALEFTGEGTVFGFSYNRPLLTDGSYSHEWSVGLQHKNFDNTSEFDNAELSGADV
                     ISIPLELAYAFERRTPDSSFYGRLAVVQEIGDDDEEYDDDRLEAESGWSALRYRLAYD
                     LLLPAEYLFHIGFSGQQTDALLISGEQFGVGGDGTLRGFEERSVTGDSGYQLNLELWF
                     PPFTAYNLRFLVFTDFAHTEFNDGELPGNEGVEFDLSSAGLGMFWSWKESLNVSLNYG
                     VITEGGGLDQSINQDGDDKLHVNAVYRF"
     gene            3921..>5055
                     /locus_tag="VIW27_07475"
     CDS             3921..>5055
                     /locus_tag="VIW27_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY5739550.1"
                     /translation="MTRLTDKLLFALLALGLSLPAPAATEVGEVAYSRGVLTGQIDGQ
                     PPRLIARGVALHNGETLNTGSKGFAIIELEDGTRMTLRPNTTFKIEEVSQERGSENAL
                     MSLIRGGLRAITGAISKRNPDAFRINTSVATIGIRGTEFDARLCATTECQAEEDASGR
                     QGERDSRVIGRIALLNGRASAREDGGSDRELSVGAAVYERDQIQTAERSFTVIAFNDK
                     TRVTLSPQSAFRIEEHEYTPEQPEDNNSFMRFLRGGMRLVSGLIGQLNPQAVRVGTPT
                     VTIGIRGTGFDLVCQGACVDENALRDPVGETLIGQFLDLFVRPVYAQAATSGMYAKAW
                     QGSIVLHLDGGSALLPNGRTAFVRNSSSRPRVVADIPALLRNFQ"
BASE COUNT          838 a         1682 c         1694 g          841 t
ORIGIN      
        1 ggcgccgatc aggcagatcc acgtgtctgc gcgcgggcac tggggggtga cgcgactgca
       61 agccagcgat taccagctcg acgcgctcgg ctacgtggtc gaattcgagt cgctcaaccg
      121 gttattgctc gaggccgtcg acgcctgcgc ggaaatcacg ctcgaggatg gcgccagctt
      181 tacgtcgctg tcgcaggacg acggtgtgcg catcgagtac cgccggcaaa aacgccagcg
      241 gcatctcgat gcgcgcctgg cgctgatcgc cgacggcgcg gactcggccg cgcgcgccgc
      301 gctcggcatc gatcaccggc gtatcgacta cgggcaatcc gcgctggtcg tcaacgtcga
      361 ggtcggccgg ccccgcgacg gactcgccta tgagcgcttc accagcgaag ggccgctggc
      421 catgctgccg ctcggcggca agcgctacgc ctgcgtctgg acctgctcgc cggcgcgcgc
      481 cgcggagctc gaagccgtcg acgatgcggc attcggcgcc gcgctgcagg attgcttcgg
      541 ctatcgcctg ggaatcgtcg agcgggtcgg caagcgggtc gcggtggcgc tgatgcgcac
      601 ccaggccgaa gcgctgtcgc gtggtcgctg cgtgctgatc ggcaatgccg ccaatgcgct
      661 gcacccggtc gccggccaga gtttcaacct gtcgctgcgc gatatcgccg ggctgcgcga
      721 attgctcgtc gaccacgagc tggcggccat cgacgacgcg gcctggcagc agatcgccgc
      781 cgtctacgcg gagtcgcgcg cagccgagca gcgccgcgta atcagctatg gcgacggcct
      841 ggtgacgctg ttttcgaacc cgctgccggt gttcgatcgg ttgcgtgccg ccggactggc
      901 ggcgctcgac ctgctgccgg cgctgaaggc acaggcggcg ttttccggca tggggctcgc
      961 gttcggcggc aaccgcctgc tgcgaggccg ccggtgagcg cgctggagac cgacatcgcg
     1021 attgccggcg ccggcatggt cggcagctgc gcggcgcatg cgttggccgc caacggcctg
     1081 cgcgtggcgc tgatcgaggc gcagccgcca cccgccgatc cgccgccggc ggacgccgcc
     1141 tacgacctgc gcgtgtcggc gatcagcccg cgttcgcgcg ccattctcga gcacctcggc
     1201 gtctggcagc aactcgatgc cgcgcgcctc tgcgactatc aaaagatgca tatctggcat
     1261 cagcacggca acgccagcgt cggcttcgac gcggtcgaac tggcgcgcga gtatctcggc
     1321 acgatcgtcg aaaaccggca gctgcaacaa gccctctacc gtgtctgcga gcggcagtcc
     1381 acgatcgagt ggttcactcc cgaccgcatc gcggcgatcg aaaccaatca cggcgacggc
     1441 gtcgagctgc gcctggaatc gggtgcccgc attgccgcgc agctgttgat cgccgccgac
     1501 gggcgcaact cgccgacccg cgaactcgtc ggtatcgacg cgcaggcggg ccgctaccgg
     1561 cagaccgcta tcgtcgccaa tgtcgacacc gaaaaggcgc atcgggagac ggcctggcag
     1621 cgcttcctgg ccaccggccc gctggccttt ctgccgctcg ccaatggcca gagctcgatc
     1681 gtctggtcct gcgacgacga cgctgtcgaa gccttgctgg cggccgacga ggcggcgttt
     1741 tgcgaagccc tcggcgacgc cttcgagtat cggctgggcc gcgtcgtgca tgccggcgag
     1801 cgcgcgagtt ttccgctcgg ctggcactac tgcgagcgct ggctggcggg gcgcgtgctg
     1861 ctgatcggcg acgccgctca cggcgtgcac ccgctcgccg ggcagggcgt caatctcggt
     1921 ttcagcgatg tcgaactgct cgcccggctg ttgcgctcgc gcgccgatct cggctctcgc
     1981 agcctgctgc ggcgctacga gcggcggcgc aagagcgaga cccgggtggc gaccgcgagc
     2041 ttcagcgcgc tgaaatggat ttacggcaac cagcagatcc tggtcagcgg cgcgcgcgat
     2101 ctcggcatgc gcgcggtcga ggccacgccg tggctgaaac gcagcctgat gcgcagggcc
     2161 atcgaaaatc tcacgtaaaa tacgcgatct cgcctatatt aagtagaaac gggaaccccg
     2221 gcgcgcgacc gaaggcggat ttcgatcggc ctgcgtgccc cggggtgatg ttcttcaatt
     2281 cgataattcg gaagaattat atagatgcgc aatggtgcag gcatattcgt cgtattgacg
     2341 ctggtatgta tgctgcccct gcgagtggcc ggccaggaca cgctggaggc agtgcgcttt
     2401 aacgtcgaac gtttcgaggt catcggcgac aacccgatcg gcaagcgtgc cgtgtcggtg
     2461 ctggcgccgt ttaccggcga gcagtacggg ctcgagggcc tgtccgcggc ccgcgacgcg
     2521 ctcgagcgcg cgatcatcga cgccggctat acctttcatc gcgtatccct gccgccgcag
     2581 gacctgttcg agggcgcggt catcctgcgc gtcgcgcgct tcgccatcgg ccgcgtcagt
     2641 gtcgagggca acctttattt cgacgacgcc aatattcggc actcggtgcc cggcctgcgc
     2701 gagggcgcga cgcccaacac gcgccggctg tcgcgctcgc tgaagctcgc caaccagcat
     2761 gcctcgaaat ccacgctgtt gcgcttcgcc gagggtaccg gcggcgatac catcgatgcg
     2821 gtactcgagg tagaagaccg cgatccgcag ctgtttttcg tcagtctcga caataccggg
     2881 cccgaagatg gcgaaatctg gcgcaccacg ctcggctacc agcacggtaa cctgttcaac
     2941 cgcgatcact cgctcaccgc gacgctgacc acggcgcccg aggatcccga ggtcaccacc
     3001 cagttcggcg tcaattacca cctgccgctg tatgcccacg gcgcgagtat cgacctgctg
     3061 ttttccgatg ccgattcggc cggcgaaacc ggcggcggcg gtgataccgg gcagggcatc
     3121 gcgccgggta ccgggggcgg ccaggcgctg gagttcaccg gcgagggcac cgtgttcggc
     3181 ttcagctaca accggccgct gctgaccgac ggcagctata gccacgaatg gtcggtcggc
     3241 ctgcagcaca agaacttcga taacacctcg gagttcgaca acgccgaact gagcggcgcc
     3301 gacgtgatca gcataccgct ggagctggcc tatgcctttg agcgccgcac gccggattcg
     3361 agcttttatg gccgcctcgc cgtggtccag gaaatcggcg acgacgatga ggaatacgac
     3421 gacgatcgcc tggaggccga gagtggctgg agcgcgttgc gctaccggtt agcctacgat
     3481 ctgctgctgc ccgccgagta cctgttccac atcgggtttt cgggccagca aaccgatgcg
     3541 ctgctgatct cgggcgagca gttcggcgtc ggcggcgacg gcacgctgcg cggcttcgag
     3601 gagcgctcgg tgaccggtga ttcgggatac cagctgaatc tcgaattgtg gtttccgccg
     3661 tttaccgcct acaacctgcg ttttctggtg tttaccgatt tcgcgcatac cgagttcaac
     3721 gacggcgagc tgccgggcaa cgagggcgtc gagttcgacc tgtcgtcggc cggtctcggg
     3781 atgttctggt cgtggaagga aagcctcaac gtgagcctca attacggcgt catcaccgag
     3841 ggcggcggtc tcgaccagag cattaatcag gacggtgacg acaagttgca cgtcaacgcg
     3901 gtctaccggt tttgaagctg atgacgcgcc tcaccgacaa actgctgttc gcattgctcg
     3961 ccctcgggct gagcctgccg gcgccagccg cgaccgaggt tggtgaggtc gcctactcgc
     4021 gcggcgtgct gaccggccag atcgacggcc agccgccgcg gctgatcgcc aggggcgtcg
     4081 cgctgcacaa tggcgaaacc ctgaacaccg gcagcaaggg gttcgcgatt atcgagctcg
     4141 aggacggcac ccgcatgacg ctgcgcccga acaccacctt caagatcgag gaagtcagtc
     4201 aggaacgcgg cagcgagaac gcgctgatga gcctgattcg cggcggcctg cgcgcgatca
     4261 ccggcgcgat cagcaagcgc aacccggacg cgtttcgcat caacacctcg gtggccacca
     4321 tcggcattcg cggcaccgag ttcgatgcac ggctgtgcgc aaccaccgag tgccaggcgg
     4381 aggaagacgc cagcggccgg cagggcgaac gcgacagccg ggtaatcggc cgtatcgcgt
     4441 tactcaatgg ccgcgccagc gcgcgcgagg acggcggcag cgaccgcgaa ctgtcggtcg
     4501 gcgccgcggt ctacgagcgc gaccagatcc agaccgcgga gcggtcgttc acggtaattg
     4561 cgttcaacga caagacgcgc gtcacgctgt cgccgcagag tgcgtttcgc atcgaggagc
     4621 acgagtacac cccggagcag cccgaggaca acaattcgtt catgcgtttt ctgcgcggcg
     4681 gcatgcgcct ggtttccggc ctgatcgggc agctcaatcc acaggcggtt cgcgtcggca
     4741 cgccgaccgt caccatcggc attcgcggca ccggtttcga cctcgtctgc cagggggcct
     4801 gtgtcgacga aaacgcgctg cgcgacccgg tcggcgagac gctgatcggg caattcctcg
     4861 acctgttcgt gaggccggtc tacgcgcagg ccgcgaccag cggcatgtac gccaaggcct
     4921 ggcagggttc catcgtgctg cacctggacg gcggctcggc actgttgccg aacgggcgca
     4981 ccgcgttcgt gcgcaacagc agcagcaggc cgcgggtggt ggccgacata ccggcgctgc
     5041 tgcgcaattt ccagg
//
LOCUS       DATCZV010000332         6318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_503005, whole genome shotgun sequence.
ACCESSION   DATCZV010000332 DATCZV010000000
VERSION     DATCZV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503005"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VIW27_07480"
     CDS             complement(<1..572)
                     /locus_tag="VIW27_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY5739551.1"
                     /translation="MTEQLQAIRGMHDILPAEMPAWLLLEDEYRSLVESYGYRQIRTP
                     IVEKTALFARSIGAVTDIVEKEMYSFEDRNGDSLSLRPENTASVVRAGLQAGLLQDQQ
                     HRLWYSGPMFRHERPQKGRYRQFHQFGAETFGMPGADIEAELILLAARFWQRLGLDDI
                     ELQINSLGSAGCRRAYRDLLVEYFRDHYAAL"
     gene            complement(586..1755)
                     /locus_tag="VIW27_07485"
     CDS             complement(586..1755)
                     /locus_tag="VIW27_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY5739552.1"
                     /translation="MNNQTDNSQSESRPAGTGIGERLQAARIQQGLSAEDIASRMHLS
                     VQIVKSIEENNFDEITAPIFVKGYLRAYARIVSLDEEEMIQQYASYYSHDDPPITTTS
                     NMAPELSVADMRIKWTTYLVIAGLAALLAAWWWNKSQDESMPISLDSQSGSTLEQTDA
                     AGGGDVPAAQADSAGDSESMTAITSVDEAQQQTPGGAATAEPAAAPEPAIAQPATETG
                     VPQAAVDSASETPADTAVVVEEAVPAAPEAVASEVTAPQAAAAEAEAPEAAAFEASAP
                     EASAPEATPSEPAAESEPAPIRLRNPVLVAPTGSDKLSLAISADTWADISDGNDFQMV
                     YHLLRAGQSLELTGSAPFSVFLGNGPGVEIRINGEPVDFSARIRSDNTTRLKIGD"
     gene            complement(1757..2500)
                     /gene="pilW"
                     /locus_tag="VIW27_07490"
     CDS             complement(1757..2500)
                     /gene="pilW"
                     /locus_tag="VIW27_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY5739553.1"
                     /translation="MRIKAIAILLLAVILQACVAVDPQQTQNQKASAVNVQLGIGYLQ
                     QNKLELASEKLTKAVRQDPDSAAAHNAYAILQDRLQQYELAKYHYERATELDPDDSQA
                     ANNYGTFLCRRGREREAEEYFIQATRNPLYRTPEYAYTNAAICLQKIGETEDALEYLR
                     KALAAKSDFAAALLAMADIKFEAGDYEAARLYVDRFHLVARPSAKSLWLAIQNTLQLD
                     GRADISELVKQLETGFPDSKEYQAWLQTQ"
     gene            complement(2490..3608)
                     /gene="rlmN"
                     /locus_tag="VIW27_07495"
     CDS             complement(2490..3608)
                     /gene="rlmN"
                     /locus_tag="VIW27_07495"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5739554.1"
                     /translation="MSMHEKINLLDLNRASLEAFFAANGEKPFRARQLMQWVHQRGVT
                     DFAQMTDMSKGLRAFLAANCCIEHPRILTCQRSADGTRKWLLGVDDSDNAIECVLIPE
                     KSRMTLCISSQLGCTLNCSFCSTAKQGFNRNLSTAEIIAQLHLVHHSLLAEGYPQGVT
                     NVVMMGMGEPLLNFDAVIAAVDMMCDDFGYALSKRRVTISTAGVVPAMERLAQLTDVA
                     LAVSLHAPYDRLRNQLVPLNRKYPIRELMRACRGYVDGRGLKITFEYVMLDGVNDSLE
                     DARRLVSLIGDIPCKLNLIPFNPYPHAAYRCSEQAAIDRFRDYTHGKGIVTVTRRARG
                     DDIDAACGQLVGSFHDRSRRSTRYQLSLQEIKGVSVAH"
     gene            3826..5313
                     /gene="thrC"
                     /locus_tag="VIW27_07500"
     CDS             3826..5313
                     /gene="thrC"
                     /locus_tag="VIW27_07500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019556228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5739555.1"
                     /translation="MIFTETRGNDGQRQPQVGFTTALLSPIASFGGLYSPQQLPPIAA
                     DFLTAHRDSSYGELALALLQHLDIDIEPAVLREAVELYRRFDDPEDPAPLRKIGERLF
                     VSELYHGPTRAFKDMALQPFGLILSSLAQRLDRQFLILTATSGDTGPAALETFRGRPN
                     IRVACLYPDGGTSDVQRLQMVTEAAPNLKVIGIHGNFDDAQSALKMLLSSPTFNRRLD
                     ELGISLSAANSVNFGRILFQMLYHFHSYLELMRSGEISAGEGIVLHIPSGNFGNALGA
                     YYARRLGLPIARIVIVSNANNVLTEWLQTGCYDLSKRQLIATTSPAMDILKSSNVERV
                     LFDLLGAARTRELMQQLDDDGRYRLEADELAQVREVFAADFCSDAESLQYIREAFAQG
                     YLVDPHTATCFKTCRAAEFAGQVNLIYSTAEWTKFAPVIEQAINGGPPKADFEALQSI
                     ASSAGIEIPATIAGLFGKPIAHPGAIDKQDIEREILAFLEASSGD"
     gene            complement(5310..>6318)
                     /locus_tag="VIW27_07505"
     CDS             complement(5310..>6318)
                     /locus_tag="VIW27_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739556.1"
                     /translation="GQIFFGPLSDSIGRRPAIVAGLLLFAAGCLLSMFAASFAQMLLG
                     RFLQGLGAAGPRIVSIALVRDRYQGRGMARVMSLVMTVFILVPVFAPAVGQGILVWAD
                     WRAIFGLFLLLALLAGGWFWIRQPETLRAEQRSVFSLSRLLLDTLAILRLRAAFGYSL
                     TMGCIFGAFVGYLSSSQQIFQVQYGLGRLFPLYFGILASVIGCASLVNARLVMRFGMR
                     RLARYALSAMSLAGLLFCGLAWIYDGHPQLLLLMAYLVFQFFLFGTLFGNLNALAMEP
                     LGHIAGLGSAVVGSLSTLISVGFGVLIAGAYDGTVMPLTLGLAVLAIGGLACLRWTEA
                     G"
BASE COUNT         1182 a         2033 c         1952 g         1151 t
ORIGIN      
        1 agcgccgcgt agtggtcgcg aaagtattcc accagcaagt cgcggtaagc ccggcggcaa
       61 ccggcgctgc cgaggctatt gatctgcaac tcgatgtcgt cgaggcccag gcgctgccag
      121 aacctcgccg ccagcaatat cagttcggcc tcgatatcgg cccccggcat gccgaaagtc
      181 tcggcgccga actggtgaaa ctggcggtaa cgcccttttt gcggccgctc gtggcgaaac
      241 atcgggccgc tgtaccagag ccgatgctgc tgatcctgca acaggccggc ctgcaggccg
      301 gcacgcacca cgctggcggt attttccggg cgcaggctga gcgaatcgcc gttgcgatcc
      361 tcgaagctgt acatctcttt ttcgacgata tcggtgaccg cgccgatgga gcgcgcgaac
      421 aacgcggtct tctcgacgat cggcgtgcga atctggcggt aaccatagga ttcgaccagc
      481 gagcgatact cgtcctccag cagtagccac gcgggcattt ccgccgggag gatatcgtgc
      541 attccacgaa tggcctgtag ctgttcggtc acgtcgactt ccggattaat cgcctatctt
      601 caggcgcgtg gtattgtcgc tgcgaattct cgcggaaaaa tcgactggct cgccattgat
      661 tcgaatctcg acaccgggcc cgttaccgag gaacaccgag aagggcgcgc tgccggtcag
      721 ttccagcgac tggccggcgc gcagcaaatg gtaaaccatc tggaaatcat taccgtcgct
      781 gatgtcggcc caggtatcgg cgctgatcgc cagcgacagc ttgtccgagc cggtcggtgc
      841 gaccagcacc gggttacgca gtcttatcgg cgccggctcg gattccgccg ccggttcgga
      901 gggtgtcgct tcgggagctg acgcttcggg agccgacgct tcgaaggctg ctgcttcggg
      961 ggcttcagcc tccgccgccg ccgcttgcgg ggctgtcact tcggacgcca ccgcttccgg
     1021 ggccgccggc acggcttcct cgacgacgac ggccgtatct gccggagtct cgctggcgct
     1081 gtccaccgcc gcttgcggca caccggtctc cgtggcgggc tgcgcaatcg cgggctctgg
     1141 agcggcagcc ggctcggccg tggctgcgcc gccaggcgtt tgctgttgcg cctcgtccac
     1201 cgacgtaatc gcggtcatcg actcgctatc gccggcgcta tccgcctgcg ccgcgggcac
     1261 atcgccgcca ccggcggcat ccgtctgctc gagcgtgctg ccgctctggc tatcgagcga
     1321 gatcggcatc gactcatcct ggctcttgtt ccaccaccag gccgcgagta atgcggcgag
     1381 acccgctatc accaggtaag tcgtccactt gatacgcatg tcggcgaccg acagttcggg
     1441 cgccatattg ctggtcgtgg tgatcggcgg gtcatcgtgc gaatagtagc tcgcgtattg
     1501 ctggatcatc tcctcttcgt cgagggatac gatgcgggca taggcgcgca ggtagccttt
     1561 gacgaaaatc ggcgcggtaa tttcgtcgaa gttattttcc tcgatcgact tgacgatttg
     1621 cacgctgagg tgcatgcggc tggcaatgtc ttcggccgac aggccctgct gaatacgcgc
     1681 cgcctgcaga cgttcgccga tgccagtgcc cgccggcctc gattcggatt gcgaattgtc
     1741 ggtttgatta ttcatcttac tgagtctgca accaggcctg gtattctttt gaatccggga
     1801 aaccggtctc cagttgcttc accagctccg agatatcggc gcgaccgtcg agttgcagcg
     1861 tattctggat cgccagccac aggctcttgg cactcggtct ggccaccagg tgaaagcggt
     1921 cgacgtagag gcgggcggcc tcgtaatcgc cggcctcgaa cttgatgtcg gccattgcca
     1981 gcaacgcggc cgcgaaatcg cttttcgccg ccagcgcctt gcgcagatac tcgagggcat
     2041 cctcagtctc accgatcttc tgcaggcaga tcgccgcatt ggtataggcg tattccgggg
     2101 tgcggtaaag cgggttgcgg gtcgcctgaa taaaatattc ctcggcttca cgttcacggc
     2161 ctcggcgaca cagaaaggtg ccatagttat tcgccgcctg cgaatcgtcc gggtcgagct
     2221 cggtcgcacg ctcgtaatgg tacttggcga gttcatactg ttgcaggcga tcctgcagta
     2281 tcgcgtaggc gttgtgcgcg gccgccgagt ccgggtcctg gcgcaccgcc ttggtcagtt
     2341 tttcgctggc cagttcgagt ttgttctgct gcaggtagcc gatacccagc tgcacgttga
     2401 cggcgctggc cttctggttc tgcgtctgct ggggatcgac cgccacgcag gcctgcaaga
     2461 tcacggccag cagcaggatc gcaatcgctt taatgcgcaa ccgacacccc cttgatttcc
     2521 tgcaggctga gctggtacct ggtgctgcgc cggctgcggt cgtggaacga tccgaccagc
     2581 tgaccgcagg cggcgtcgat atcgtcgccg cgcgcacgcc gggttacggt gacgatgccc
     2641 ttgccatggg tgtagtcgcg aaagcggtcg atggctgcct gctccgagca ccggtaggcg
     2701 gcatgcgggt aaggattgaa aggtatcaga ttgagcttgc agggtatgtc accgatcagg
     2761 ctgaccagtc ggcgcgcgtc ctcgaggctg tcgttgacgc cgtcgagcat cacgtattcg
     2821 aaggtgatct tgaggccgcg gccgtcgaca tagccgcggc aggcacgcat cagttcgcga
     2881 atcgggtatt tgcggttgag cggtaccagc tggttgcgca ggcgatcgta gggcgcgtgc
     2941 agcgaaaccg cgagcgccac gtcggtcagc tgcgcgagcc gctccattgc cggcaccacg
     3001 ccggcggtgc tgatggtcac gcggcgcttg ctgagtgcgt agccgaaatc atcgcacatc
     3061 atgtcgaccg ccgcgatcac cgcgtcgaaa ttcagcagcg gttcgcccat gcccatcatg
     3121 accacgttgg tgaccccctg cggatatccc tcggcaagca ggctgtgatg caccaggtgc
     3181 agctgggcaa tgatctccgc ggtgctcaga ttacggttga agccctgctt ggcggtcgag
     3241 cagaaactgc agttcagcgt gcagccgagc tgcgacgaaa tgcatagcgt catgcgcgat
     3301 ttttccggga tcagcacgca ctcgatcgcg ttatcgctgt cgtcgacacc gagcagccac
     3361 ttacgggtgc cgtcggccga acgctgacag gtcaggattc gcgggtgctc gatgcagcag
     3421 ttggcggcca gaaacgcgcg caagcccttc gacatatcgg tcatttgcgc gaagtcggtg
     3481 accccgcgct gatgcaccca ctgcattagt tgccgggcac gaaacggttt ttccccgttg
     3541 gcggcgaaga atgcctccag cgaggctcga tttaaatcga gtagattgat tttctcgtgc
     3601 atcgacatcg ggttgaaata gaatactctg gctcggccaa tagcgcccgg cctgacctgc
     3661 cgccgggtta atcgccgaat tttacgcata aatgtcgcgc agtagaagtg aaatgctcga
     3721 cagcggccac tatttaactg catttgctgt ttttatgcgg cgaaaccgcg acttagcgga
     3781 tataatccgc gctttcgcca ccttcgacga gtcagccagg cgcgaatgat ttttaccgaa
     3841 acccggggca acgacggtca gcgacaaccc caagtcggct tcaccacggc gttgctgtcg
     3901 ccgatcgcct cgtttggcgg tctttacagc ccacaacagt tgccgccgat agcggcggat
     3961 ttcctcaccg cgcaccgcga cagctcctac ggcgaactgg ctttagcgct gctgcagcac
     4021 cttgacatcg atatcgagcc cgcggtgctg cgcgaggcag tcgaactgta tcggcgcttc
     4081 gacgaccccg aagacccggc tccgctgcgc aagatcggcg aacgcctgtt cgtcagcgag
     4141 ctgtaccacg gtccgacgcg cgccttcaag gacatggcgc tgcaaccctt cgggctgatt
     4201 ctgtcgtcgc tggcgcaacg gctcgaccgc cagttcctga tcctgaccgc gaccagcggc
     4261 gacaccggac cggcggcgct ggaaaccttt cgcgggcggc ccaatattcg cgtcgcctgc
     4321 ctttatccgg acggcggcac ctccgacgtc cagcggctgc agatggtcac cgaagcggcg
     4381 cccaacctga aagtcatcgg cattcacggc aatttcgacg acgcgcagtc ggcgctcaag
     4441 atgctgctgt cgtcgccgac cttcaaccgc cggctcgacg agctgggcat ctcgctgtcg
     4501 gcggccaact cggtcaattt cggccgcatc ctgttccaga tgctgtacca ctttcacagc
     4561 tacctcgaat taatgcgcag cggcgaaata agcgccggcg aaggtatcgt gctgcacatt
     4621 ccgagcggca acttcggcaa tgcgctcggc gcctattacg ctcgccgcct gggtctgccg
     4681 atcgcacgca tcgtgatcgt gtcgaacgcc aacaacgtgc tcaccgagtg gctgcagacc
     4741 gggtgctacg acctcagcaa gcgtcagctg atcgccacca cctcgccggc gatggatatt
     4801 ctcaagtcat ccaacgtcga gcgggtgctg ttcgacctgc tcggcgcggc gcgcacccgc
     4861 gaactcatgc aacagctcga cgacgatggg cgttaccggc tcgaggccga cgagctggcg
     4921 caagtgcgcg aggtgtttgc cgcggacttc tgcagcgatg ccgagagcct gcagtacatc
     4981 cgcgaggcgt tcgcgcaggg ctacctggtg gatccgcaca ccgcgacctg tttcaagacc
     5041 tgccgcgcgg cggagttcgc cgggcaggtc aacctgatct actcgaccgc cgagtggacc
     5101 aagttcgcgc cggtcatcga gcaggcgatc aatggcggcc cgccgaaagc cgatttcgag
     5161 gcactgcagt cgatcgcgtc cagcgccggc atcgaaatac cggccacgat cgccgggttg
     5221 ttcggcaagc ccatcgccca cccgggggcg atcgacaagc aggacatcga gcgggaaatc
     5281 ctcgccttcc tcgaggcgag cagcggcgat tagccggcct cggtccagcg caggcaagcc
     5341 aggccgccga tcgccaacac cgccaatccg agtgtcagcg gcatcaccgt gccgtcgtag
     5401 gcgccggcga tcagcacgcc gaagccgacc gaaatcagtg tcgacagcga acccacgacc
     5461 gccgagccga ggcccgcgat atggcccagc ggctccatcg ccagcgcgtt gagattgccg
     5521 aacagcgtac cgaacaggaa gaactggaag accaggtaag ccatcagcag caacagctgc
     5581 ggatggccat cgtagatcca ggccaggccg cagaacagca gcccggcgag gctcatcgcc
     5641 gacagcgcgt agcgcgcgag gcggcgcatt ccgaagcgca tcaccagccg cgcgttgacc
     5701 agcgaagcac agccgatgac gctcgccagt atgccgaaat acagcgggaa caacctgccc
     5761 agcccgtact gcacctggaa gatctgttgc gagctgctca ggtagccgac gaaagcaccg
     5821 aatatgcagc ccatggtcag cgaatagccg aacgccgcgc gcaggcgcaa gatggccagc
     5881 gtatccagca gcaggcgcga cagcgagaaa acgctgcgct gctccgcgcg cagggtctcc
     5941 ggctgacgga tccagaacca gccgccagcg agcagcgcga gcaacaggaa caggccgaaa
     6001 atcgcccgcc agtcggccca gacgagtatt ccctggccga ccgcgggtgc gaataccggc
     6061 accaggatga aaacggtcat caccaacgac atcacgcgcg ccatgccgcg cccctggtaa
     6121 cgatcgcgca ccagcgcgat cgagacgatg cgcgggccgg cggcgccgag cccctgcaaa
     6181 aagcgaccca gcagcatttg cgcaaagctc gccgcgaaca tcgacaacag gcagccagcc
     6241 gcgaacaaca gcaggccggc gacgatcgcc gggcgacggc cgatggaatc cgacagcggg
     6301 ccgaagaaaa tctgcccg
//
LOCUS       DATCZV010000333         5707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_609202, whole genome shotgun sequence.
ACCESSION   DATCZV010000333 DATCZV010000000
VERSION     DATCZV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609202"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1086
                     /locus_tag="VIW27_07510"
     CDS             184..1086
                     /locus_tag="VIW27_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5739557.1"
                     /translation="MWDNLSILAVAPVFNVQLILNGVLVGAVFALAAYGLALVWGVMN
                     VKNLAQGDFVIAGGYVSWWLVQLGFPPLLGVPAAFVIMAGIGWIAYKLVIKRVIERDL
                     FTSLLATFGLAIMMAQILNLLFGSDTQSVDLDFDTLYFADGLIDVPIAKLIGVCMAAV
                     LAAGVILFMKNSRMGQAIRATSQDARAARVLGIDTEKVYAFTFSLNAAICGAAGAIIV
                     MVWVIQPFYGITHSIRAFVIVTAAGLGNLPGVIAAGLGIGALEQYGAHIFGIGYQQAI
                     AVMLLLLVLFIRLLQQMRHRQVLK"
     gene            1086..2123
                     /locus_tag="VIW27_07515"
     CDS             1086..2123
                     /locus_tag="VIW27_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5739558.1"
                     /translation="MGKNKIILYSAILAIAIVAPFAFPDFGTQLATLWLMIIVALTWD
                     MTGGQMGYNSLGNIFFYGTGMYVAAVIAIGMVYDVGAYTDASGGGIFKFTDAQYFGGL
                     FIGIIGAGLFCSATAVIIGFLTFGLRGPYFAIGTLGVAIAAGELVSNWDWIGGGQGIA
                     LPVYPGDLDVGKVFFYFLFAVTGVVLFLFCKWLYGTRFGAAINAIRDDEEKADAMGIH
                     TLRYKLVTWAMAAFFVGIAGGISAFQLIHFEPLETAYQTINLGIFMVVCVLLGGKGTL
                     WGPVIGAILFHVFKEVTWNYFLGWQWVALGALIIVTVVYFKDGVMGYLAYVKPEWFGI
                     RVERKTGEEGT"
     gene            2120..2866
                     /locus_tag="VIW27_07520"
     CDS             2120..2866
                     /locus_tag="VIW27_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739559.1"
                     /translation="MSDIIIDVNNVSKSFGGVKANTNVSMQVERGGITGLIGPNGSGK
                     TTLFNSIVGYHPIDGGSIKFEGEEISTFSVQKIARRGLLRTFQQTRIYSKMNCVENML
                     ISLPHRKMSLLDALKKNTREDYERADQLLDFVGLYEKRYLMSGDLSFGQQKLLEFAMA
                     LMNEPTCLLLDEPTAGINPTLINGLINRLKRANEEFGITLFVIEHNMRVIMNLAESIY
                     CLAHGEMLAHGTPEEIQSDQKVIDAYLGAH"
     gene            2867..3748
                     /locus_tag="VIW27_07525"
     CDS             2867..3748
                     /locus_tag="VIW27_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739560.1"
                     /translation="MSEQTPEERNKTRGYHGGGVDTVISVDEVARQAAAIAEEISLDQ
                     LDALAGNEPYVAINDLVAGYGQMEILHNLNLRVGRAQSLCLIGPNGAGKSTILHAIFG
                     FNRIFSGSIQVGAGSDAIDVTRLSPNQKLSQAGIAYILQDKSIFPGMTVEENLWMGGF
                     LKEKPQEAKEAAEKVFEKYDRLAKRRKHKAGVLSGGERRLLEISRALVMNPEVLLVDE
                     PSIGLEPRFIDMVFEILDDLQNNEGKTIIMVEQNAKKGLEFADIGYVLVSGQLAIAGK
                     GDDLLQNPKVGELFLGG"
     gene            4042..5106
                     /locus_tag="VIW27_07530"
     CDS             4042..5106
                     /locus_tag="VIW27_07530"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY5739561.1"
                     /translation="MASTKTYRIAVIPGDGIGNEVVPEGLKVLDAIAPRFGIEFDYGH
                     FDWSCERYLETGAMMPEDGIEQLSKFDAIFLGAVGFPTVLDHVSLWGLLIPIRREFRQ
                     YVNLRPVRLFEGMRCPLANPGKIDFYVVRENNEGEYSNVGGRLYAGTEDEMAMQQSIF
                     TRRGSDRILKYAFDLAQSRPRKHLTSATKSNGIMHTMPYWDERVAAMAEKYPDIEVDK
                     YHIDILVSHFVNKPEIFDVVVGSNLFGDILSDLGPGVTGTIGIAPSANINPERDFPSM
                     FEPVHGSAPDIAGQGIANPIGQIWTAAMMLDHLGHRDAHDAIVTAIEDTIREKQSLTP
                     DMGGTSSCSDCGSAIAARLG"
BASE COUNT         1135 a         1732 c         1709 g         1131 t
ORIGIN      
        1 cggcgggcaa caacatcgcc aagccgatgg tgctgcgcca ggtgcagagc ggccagtaca
       61 acgtcgtcgc gcccaccaag tgggcctcgc acaagctggc gtacccgcgc aaggctggca
      121 actagacccc ggtagaggag ggcccgaccg cgccggccgg aaacggccgg cgcggccttc
      181 gtcatgtggg ataacctgag cattctcgcg gttgcaccgg tcttcaacgt gcaactgatc
      241 ctcaacggcg tgctggtcgg cgcggtgttc gcgctcgccg cctacggcct ggcgctggtg
      301 tggggcgtca tgaatgtcaa gaacctcgcg cagggcgact tcgtcatcgc cggtggctac
      361 gtctcctggt ggctggtgca gctcggcttt ccgccgctgc tcggagtgcc ggcggcgttc
      421 gtgatcatgg ccggcattgg ctggattgcc tacaagctgg tgattaaacg tgtcatcgag
      481 cgcgacctgt tcacgtcgct gctcgcgacc ttcggcctcg cgatcatgat ggcgcaaatc
      541 ctgaacctgc tgttcggctc ggacacgcaa tcggtcgacc tcgacttcga cacgctgtat
      601 ttcgccgacg gcctgatcga tgtgccgatc gccaagctga tcggcgtttg catggccgcg
      661 gtgctcgccg ccggcgtcat cctgttcatg aaaaacagcc gcatggggca ggcgattcgc
      721 gccacttcgc aggacgcccg cgccgcgcgc gtgctcggca tcgacaccga gaaagtctac
      781 gccttcacgt tctcgttgaa cgccgcgatc tgcggcgcgg ccggcgcgat catcgtcatg
      841 gtctgggtca tccagccgtt ttacggcatc acccactcga tccgcgcctt cgtcatcgtc
      901 accgcggccg ggctcggcaa cctgcctggc gtcatcgccg ccgggctggg catcggcgcg
      961 ctcgagcagt acggcgcgca catcttcggc atcggctacc agcaggcgat cgcggtcatg
     1021 ctgttgctgc tggtattgtt catccgcctg cttcagcaaa tgcgccatcg acaggtgctg
     1081 aagtaatggg caagaacaaa atcattctat attcggcgat cctggccatt gcgatcgtcg
     1141 cgcccttcgc gttccccgat ttcggcaccc agctggcgac gctgtggctg atgatcatcg
     1201 tcgcgctgac ctgggacatg accggcgggc agatgggtta taactcgctc ggcaatatct
     1261 tcttctacgg caccggcatg tacgttgcgg cggtgatcgc gatcggcatg gtctacgatg
     1321 tcggcgccta caccgatgcc tcgggcggcg gcatcttcaa gttcaccgac gcgcagtatt
     1381 tcggcgggct gttcatcggc atcatcggcg ccgggctgtt ctgttcggcg accgcggtca
     1441 tcatcggttt cctgaccttc ggcctgcgcg gtccgtactt cgcgatcggc acgctcggcg
     1501 tcgcgatcgc ggccggcgag ctggtgtcga actgggactg gatcggcggc ggccagggca
     1561 tcgcgctgcc ggtttacccc ggcgacctcg acgtcggcaa ggttttcttc tacttcctgt
     1621 tcgcggtgac cggggtcgtg ttgttcctgt tttgcaaatg gctgtatggc acgcgcttcg
     1681 gcgcggcgat caacgcgatt cgcgacgacg aggaaaaagc cgacgccatg ggcatccaca
     1741 cgctgcgcta caagctcgtg acctgggcga tggcggcatt cttcgtcggc atcgccggcg
     1801 gcatctcggc cttccagctg attcatttcg agccgctgga aaccgcctac cagaccatca
     1861 acctgggaat ctttatggtc gtctgcgtgc tgctcggcgg caagggcacg ctgtgggggc
     1921 cggtcatcgg cgcgatcctg ttccacgtct tcaaggaagt cacctggaat tattttctcg
     1981 gctggcaatg ggtggcgctc ggtgcgctga tcatcgtcac cgtggtctac ttcaaggacg
     2041 gcgtcatggg ttacctggcg tacgtcaagc ccgagtggtt cggcattcgc gtcgagcgca
     2101 agaccgggga ggaaggcacg tgagcgacat catcatcgat gtgaataatg tctccaagtc
     2161 gttcggtggc gtcaaggcga acaccaatgt ctcgatgcag gtcgagcgcg ggggcatcac
     2221 cggcctgatc ggtcctaacg gctccggcaa gaccacgctg ttcaattcca tcgttggtta
     2281 ccacccgata gacggtggct cgatcaagtt cgagggcgag gaaatttcga ccttttcggt
     2341 gcagaaaatt gctcgccgcg gcctgttgcg cacctttcag caaacccgta tctacagcaa
     2401 gatgaactgc gtcgaaaata tgctgatctc gctgccgcat cgcaagatga gcctgttgga
     2461 tgcgctgaag aagaataccc gcgaggatta cgagcgtgcc gaccagttgc tcgatttcgt
     2521 cgggctctac gaaaagcgtt acctgatgag cggcgacctg tccttcggtc agcaaaagct
     2581 gctcgaattc gcgatggcgc tgatgaacga gccgacctgc ctgctgctcg acgagcctac
     2641 cgcgggcatc aatccgacgc tgatcaatgg cctgatcaac cgcctcaagc gcgccaacga
     2701 ggaattcggc atcacgctgt tcgtcatcga acacaacatg cgcgtgatca tgaacctggc
     2761 cgagtcgatc tattgtctcg cgcacggtga gatgctcgca cacggcacgc ccgaggagat
     2821 tcagagcgac caaaaggtaa tcgacgccta cctgggggca cactagatga gcgagcaaac
     2881 tccggaagag cgcaacaaaa cccgcggtta ccacggcggc ggcgtcgata cggtcatttc
     2941 ggtcgacgag gtcgcgcgcc aggcagcggc gatcgccgag gaaatcagcc tcgatcagct
     3001 cgatgcgttg gccggtaacg agccttatgt ggcgatcaac gacctggtag ccggctacgg
     3061 gcagatggag atcctgcaca atctgaacct gcgcgtcggc agggcccagt cgctgtgcct
     3121 gattggcccc aacggtgccg gcaagtccac catcctgcac gcgatattcg gcttcaaccg
     3181 cattttttcg ggctcgatac aggtcggcgc gggcagcgac gcgatcgacg ttacgcgcct
     3241 gtcgccgaac cagaagctct cgcaggccgg catcgcctat atcctgcagg acaagtcgat
     3301 ctttcccggt atgaccgtgg aagaaaacct gtggatgggc ggtttcctca aggaaaagcc
     3361 gcaggaggcc aaggaggcgg cagaaaaggt gttcgaaaaa tacgatcgtc ttgccaagcg
     3421 ccgcaagcac aaggccgggg tgctttccgg cggcgagcgg cgcctgctgg agatctcgcg
     3481 tgcgctggtc atgaacccgg aagttctgct ggtcgatgaa ccgtcgatcg gcctcgaacc
     3541 gcgcttcatc gacatggttt tcgagattct cgacgacctg cagaataacg aaggcaagac
     3601 catcatcatg gtcgagcaaa atgccaagaa ggggctcgag ttcgccgata tcggttacgt
     3661 gctggtgtcg gggcagctgg cgatcgccgg caagggcgac gacctgttgc agaatcccaa
     3721 ggtcggcgaa ctgttcctcg gcggttagcc ccgccgtcat gccgggcccc gatccggcat
     3781 ccatagatgc aagaagattg cggctcgaag gccgcaatga cgagcgccgg cgaatgtcat
     3841 cgggataatg cgctgcctcg tcatgccggg ccccgacccg gcatccatag atgaaaaaag
     3901 attgcggctc gaaggccgca atgacgagtg ccggcgaatg tcatcggcat aattcgctgc
     3961 ctcgtcatgc cgggccccga cccggcatcc atagctgaaa aaagattgcg gctcgggggc
     4021 cgcaatgaca ggtattaacg aatggcttcc acaaaaacct atcgaatcgc ggtaataccc
     4081 ggcgacggca tcggcaacga ggtcgtgccc gagggactca aggtgctcga tgcgatcgca
     4141 ccgcgcttcg gtatcgagtt cgactacggc cacttcgact ggagctgcga acgttacctc
     4201 gaaaccggcg cgatgatgcc ggaggacggc atcgaacagc tgtcgaagtt cgacgccatc
     4261 tttctcggcg cggtcggttt tccgaccgta ctcgaccatg tctcgctgtg gggcctgctg
     4321 attccgattc ggcgcgaatt ccggcaatac gtcaacctgc gcccggtgcg cctgttcgag
     4381 ggtatgcgct gcccgctggc gaacccgggc aagatcgatt tctacgtggt gcgtgaaaac
     4441 aacgagggcg agtactcgaa cgtcggcgga cggctgtatg ccggcaccga ggacgagatg
     4501 gcgatgcagc agtcgatctt tacccgccgg ggcagcgacc ggatattgaa atacgctttc
     4561 gatctcgcgc agtctcgacc gcgcaagcac ctgacctcgg caaccaagtc caacggcatc
     4621 atgcacacca tgccctactg ggacgagcgc gtcgcggcga tggccgagaa atacccggat
     4681 atcgaggtcg acaagtatca catcgatatc ctggtctcgc atttcgtcaa caagcctgag
     4741 attttcgacg tggtggtcgg ctcaaacctg ttcggcgaca tcctctccga cctcggcccc
     4801 ggcgtgaccg gcaccatcgg tatcgcgccg tcggccaata tcaatcccga gcgcgatttc
     4861 ccgtcgatgt tcgaacccgt gcacggttcc gccccggaca tcgccgggca gggtatcgcc
     4921 aacccgatcg gccagatctg gaccgcggcg atgatgctcg accatctcgg tcaccgcgac
     4981 gcccacgacg ccatcgtcac cgcgatcgag gacaccatcc gtgaaaagca gagcctgacc
     5041 ccggacatgg gtggcacctc cagctgcagc gattgcggct cggccatcgc cgcccgcctg
     5101 ggctgattcc tgcgtctttc gagcactgcg aaaatcggaa aatcggcgag gccacggatg
     5161 gctctgcgta gatcgttacc ggcggcaggc tggattacgc tatcgcaatc gaaggcccga
     5221 ataacaaacg gcaatctcga atcgcctcgc tccggcggcg ccccttgatc cgcggaagcg
     5281 ccgatagctt tcccgccaga gcggccgctc gaaaagatgc caggagagac gtagttcgac
     5341 ccttttgaga cgcgcgattg acttactccc ctcgtgtgcg gtgcgcttta aattacaggg
     5401 gtacggtcaa attatcctga taaatgcatc agatggtcac aaccgaatag gtaatggcag
     5461 acgggtataa catcatgaaa tgcgcaggat gccgcgaaaa acaaaaccac gaaacccagt
     5521 gaataaataa ttgacctgta gtgccgatct atccaagtat tgattgcatg catgtctctt
     5581 cccctccgac agcttttggc atctctgtct tttaaataat cagaaaatca attaatgatc
     5641 tgcttaatat cgccccatac tgttgatgcg aatcaacact gactccgtat ttaaggaaat
     5701 ttcgaac
//
LOCUS       DATCZV010000334         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_715157, whole genome shotgun sequence.
ACCESSION   DATCZV010000334 DATCZV010000000
VERSION     DATCZV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715157"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..955)
                     /locus_tag="VIW27_07535"
     CDS             complement(113..955)
                     /locus_tag="VIW27_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015499027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY5739562.1"
                     /translation="MQIRRFIQCDVFTPVPTLGNALAVVVDGDELSEQVMQQFAAWTN
                     LAETTFILPPLDAQADYRLKIYTPVREVLFAGHPTLGSCAAWLHCGGAPRQSDRVRQE
                     CGIGIVEIDISNPGMPAFIAPPTRVRSIQESALEAICAALEITPGQVLHTAELDNGPV
                     FQVLLLDSAESVLALDSSRVRRPAFRSIGVLGAHPPGGECDYEVRHLAPSSGMSEDPI
                     TGSLNSALAHWLQAEGRLPRAVTVAQGTSIGRHGRVAIEPLDRRNVKIGGQTHILIEG
                     QVAL"
     gene            1293..>2143
                     /locus_tag="VIW27_07540"
     CDS             1293..>2143
                     /locus_tag="VIW27_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HEY5739563.1"
                     /translation="MEKRPYSKSLRGKITNRTLFIGIVPVLLVGVFAWFSLNQLTSSA
                     NQQLDKSRSELLDSVVGSNLSTTSARIVSQLDAFMLERISDVVVWASAPIIIQAAKAA
                     AEAHQKVGLVDQSIDEIEARFKKRKSLNVSPAANRFLIQQIRRSTHFGEVFFTDKHGF
                     NTALTNPTSDFVQRDENWWKTAWENGISVGEVEFDDSAGIWSIDISVRIDDPASGRSL
                     GVMKAVLGVSLIQEVADNGARDIPGGTVTVLSSGGLLLAETGTKHDKKRIMVDTVNMR
                     DSADPAI"
BASE COUNT          467 a          645 c          597 g          434 t
ORIGIN      
        1 gcccaggtca tagtgccgat agtcgagtgc cagttcgccg agaatccaca acactttctg
       61 cacgttgtag gcattttttc gcccccatag ttcgatcatc gtaacctcca cgtcagagcg
      121 cgacctgacc ttcgatcagg atatgggttt gcccgcctat cttgacattg cgtcgatcaa
      181 gcggctcgat ggcaacgcgt ccgtggcgtc cgatgctggt accctgggcg accgtcacgg
      241 cccgcggtaa acgaccctcg gcctgcagcc agtgcgccag cgcagagttg agagaaccgg
      301 tgatcgggtc ctcgctcatt ccgctcgaag gcgccaggtg gcgcacctcg taatcacatt
      361 cgccccccgg cgggtgcgcg ccaagcacac cgatcgacct gaaggctggc cggcgcacgc
      421 gcgacgaatc gagcgccagc accgactcgg cggagtccag cagcagcacc tgaaataccg
      481 ggccattgtc gagctccgcc gtatgcagaa cttgcccggg cgttatctcc agcgcagcgc
      541 aaatagcctc cagcgctgac tcctggatcg aacgaacgcg ggtcggcggc gcgatgaagg
      601 ccggcatccc cgggttgctg atatctattt ccacaatccc gatgccgcac tcctgccgca
      661 ccctgtccga ctgccgcggg gcaccaccac agtgcagcca ggcggcacag ctacccagcg
      721 tcgggtggcc cgcaaacaac acttctcgca ccggcgtgta gattttcaag cggtagtcgg
      781 cctgcgcgtc cagcggcggc aggatgaaag tggtttcggc aagattggtc caggcggcaa
      841 attgttgcat tacctgctca gacaattcat cgccatcgac caccaccgcc agcgcgttac
      901 ctagcgtcgg tacgggggtg aacacgtcac actggataaa gcggcgtatc tgcatcttcg
      961 aatctcgttc ctctgttgta ggcgccgcgt tatgacgctc ttacttcgtc ttaattcgcg
     1021 ctatggaggc gtcaaactta acgccgacaa agccccaaac tatctcggcc atggtctata
     1081 tatagctatc aagagcatga tactaacgac ttgtcgacca cactagttaa accattttat
     1141 caagtataat aaataagtgt ttgtatttaa tgtacaaacg tctttccaag gggtctgcat
     1201 cttcattgtt cggtaaagcg ttcatgcatt atgagatatt cttcagcctg taaccgcggt
     1261 gcaaacctgg cgtgccgcag cgagtcacga cgatggaaaa acgaccttat agcaaaagcc
     1321 tgcgcggcaa aatcactaac cgcaccctgt ttatcggcat cgtgccggta ctgctggttg
     1381 gtgtatttgc ctggttcagc ctgaaccagt taacttcgag cgccaaccag cagctcgaca
     1441 agagccggtc cgaactgctc gactcggtcg tgggtagtaa cctcagcacc acctcggcac
     1501 gcatcgtaag ccagctcgac gctttcatgc tggaacggat ttcggacgtc gtggtctggg
     1561 cctcggcgcc gattatcata caggcggcca aggcggcggc agaggcgcac caaaaggtag
     1621 gcctggtcga ccagtccatc gatgagatcg aagcccgttt caagaagcgc aagagcctga
     1681 acgtatcgcc cgccgccaac cgttttctga tccagcaaat caggcgttcg acccatttcg
     1741 gtgaagtttt ctttaccgat aagcatggtt ttaacaccgc gctgaccaac cctacctcgg
     1801 atttcgtgca gcgtgacgaa aactggtgga aaacggcctg ggagaacggt atctccgtgg
     1861 gcgaagtcga gttcgacgac tcggccggaa tctggtcgat cgatatctcg gtgcgcatcg
     1921 acgatccggc gtcgggcaga agtctcggtg tcatgaaggc ggtgctcggc gtgagcctga
     1981 tccaggaagt tgccgacaac ggcgcgcgcg atattcccgg cggcacggtt accgtgctca
     2041 gcagcggcgg gctgttactg gccgagaccg gcaccaaaca cgacaagaaa cgcatcatgg
     2101 tggatacggt gaacatgcga gacagcgccg acccggcgat cca
//
LOCUS       DATCZV010000335         4738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_132876, whole genome shotgun sequence.
ACCESSION   DATCZV010000335 DATCZV010000000
VERSION     DATCZV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132876"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..363
                     /locus_tag="VIW27_07545"
     CDS             70..363
                     /locus_tag="VIW27_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY5739564.1"
                     /translation="MSAYVLAQIQINDTEKYQDYLAGFMPIFKRHGGELLATSKNPTI
                     VVEGSWAYPATVIMKFPSVKAARAWCDDPDYKALAEHRHASADANLVIVEGIS"
     gene            complement(410..1624)
                     /locus_tag="VIW27_07550"
     CDS             complement(410..1624)
                     /locus_tag="VIW27_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY5739565.1"
                     /translation="MSQIQRPDFFQLENGSKAVLPFAASEYEARLGKLRALMAEHDVD
                     AMLLTSMHNIAYYSGFLYCSFGRSYACVVTAERCVTVSANIDLGQPWRRGYGDNLIYT
                     DWQRNNYWRAVSSLMPRAKSLGIEGDHLTLAARATLGEFIDAETVDLNREIMGLRMIK
                     SAAEIELIKGGAHTADIGGAAIRDAIRVGTREIDVAMAGRDAMELEIARAYPDSELRD
                     TWVWFQSGINTDGAHNPVTTRALQAGDILSLNAFPMISGYYTALERTLFLGEPDAESL
                     RIWQANVGAHELGISLIKPGATCSGICEELNRYFADLDLLQYRTFGYGHSFGILSHYY
                     GREAGLELREDIDTMLEPGMVVSMEPMLTIPQGRPGAGGYREHDILVITEDGAENITG
                     FPFGPEHNIVRA"
     gene            1806..2279
                     /locus_tag="VIW27_07555"
     CDS             1806..2279
                     /locus_tag="VIW27_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5739566.1"
                     /translation="MTSNTFTIRPAATGDHAQWKPLWQGYLEFYRSSLAPEVTDLLWQ
                     RILDPQHEIRGLLAEADGGLTGLVHFFPHAHTWNSAPVCYLNDLFVLPSVRGGGVGRA
                     LIEAVVDEARRQEWTRVYWHTQHDNAVARSLYDKVTGGTDGFVNYTLILAKDSPQ"
     gene            complement(2485..3045)
                     /locus_tag="VIW27_07560"
     CDS             complement(2485..3045)
                     /locus_tag="VIW27_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HEY5739567.1"
                     /translation="MADRVSALAGHYRPGHHGDHERTGVILQDMQGLVLHQVAAWRDS
                     IDAVGEKLAGLIGADATAGPGAAVSGGQGSMLRIEPMKWWLVDIEAPAFAAEQAMTLD
                     LSHSRTRLRVSGDAAAEFLNRHLPLDLRDASFPVGSVASSATHHVGVTLWRSADGYEL
                     FIPRGFALSLWEGFVESAAQFGLEIR"
     gene            complement(3038..>4738)
                     /locus_tag="VIW27_07565"
     CDS             complement(3038..>4738)
                     /locus_tag="VIW27_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019294994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY5739568.1"
                     /translation="VKPVWNADNACFVVPETDEPIQVAGSAAGIWNTDACFDSGAAAG
                     ARAVKALGGKARAKAMPAIGGWEHPIRPLYEIRDPARKTKAFVDPQHDVTADDVRLAH
                     REGFVSVEHLKRYTTLGMATDGGKVGNIIGLALMAEALGVEIPEVGTTTFRPPYTPVA
                     IGALKGRNVDQHFRPLRRTPMHDWNLEQGATMTMAGLWHRPWYFARQGEGISEAYVRE
                     TETTRAAVGLCDVTSLGKIAVQGPDATEFLNRIYTNPFARLPIGKARYGIMLRDDGIV
                     MDDGTTWRLSETDYFMTTTTAHAGKVMAWLEELLQTRWQELRVHVTSVSEQWAGSAVA
                     GPQSRAVLKACVEDASVMDNEVLPFMGVIESSLKGGIPCRIARISFSGEMAYEAYVPS
                     DYATAMMELLWTEAQQHDGCLYGLEALGAMRIEKGHVTGAELDGRVSIDDAGLGKMAN
                     PDKLYIGKAMRERPELLREDRPRLVGIKPVDRSQKFNAGAILCAAGKVSGLGEGWITA
                     VTHSPALGHWIGLGFVAGGHQAWEGKTLVAADPVRKGNIDVEVVSPHMYDPQGERMHG
                     "
BASE COUNT          846 a         1527 c         1529 g          836 t
ORIGIN      
        1 gtgaagaacc aggtgttcat ctgctggcag gatgtttaag gacccttcaa tttacgagag
       61 gacgataaaa tgagcgctta tgttctggcc cagatacaaa tcaatgatac ggagaaatac
      121 caagactatc tggcgggttt catgccaatt ttcaaaaggc acggtggcga actactggcg
      181 acttcgaaga acccgaccat cgtggtcgaa ggcagctggg cctatccggc cacggtaatc
      241 atgaaattcc ccagtgtaaa agccgcccgg gcatggtgtg acgacccgga ctacaaggcc
      301 ctcgcagagc atcgccacgc ttccgccgat gcgaatctgg tcatcgtcga agggatatcc
      361 tgaaggtcat aaacaggggg cacgatcgcg ccccctgtgt cgctgtcgat caggcgcgga
      421 cgatgttgtg ctcggggccg aaggggaaac cggtgatgtt ctccgctccg tcctcggtaa
      481 tcacgagaat gtcgtgctcg cgataaccgc cggcgccggg ccggccttgc ggtatcgtca
      541 gcatcggttc catcgacacc accatgcccg gttcgagcat ggtgtcgata tcctcgcgca
      601 gttcgagacc ggcctcgcgg ccgtagtaat gcgacaggat gccgaaggag tggccgtaac
      661 cgaaagtgcg gtactgcagc aggtcgagat cggcgaaata acggttgagt tcctcgcaga
      721 tccccgagca ggtcgcgccc ggtttgatca gcgagatgcc tagctcgtgg gcgccgacgt
      781 tggcctgcca gatacgcagc gactcggcat cgggttcgcc gaggaacagc gtgcgctcga
      841 gcgcggtgta gtagcccgag atcatcggga aggcattgag gctgaggata tcgccggcct
      901 gcagcgcgcg cgtggtcacc gggttgtgcg caccgtcggt attaatgccg gactggaacc
      961 agacccaggt atcgcgcagc tcgctgtccg ggtaagcgcg cgcgatctcc agttccatcg
     1021 catcgcggcc ggccatcgcg acatcgattt cgcgggtgcc gacgcggatc gcgtcgcgaa
     1081 tcgccgcgcc gccgatatcg gcggtgtgcg caccaccctt gatcagctcg atttcggccg
     1141 ctgacttgat catccgcagg cccatgattt cgcggttcag atcgacggtc tcggcgtcga
     1201 tgaactcacc gagggtcgcg cgcgcggcga gcgtcaggtg gtcgccctcg ataccgagcg
     1261 atttcgctcg cggcatcagc gagctgaccg cgcgccagta attgttacgc tgccagtcgg
     1321 tatagatcag gttgtcgccg taaccgcgcc gccacggctg gccgaggtcg atgttcgccg
     1381 acaccgtgac gcaacgctcg gcggtcacca cgcaggcata ggagcggccg aacgagcagt
     1441 acagaaaacc cgagtagtag gcgatgttgt gcatcgaggt cagcagcatc gcgtcgacat
     1501 cgtgctcggc catcagtgcg cgcaacttgc cgaggcgcgc ctcgtactcg ctggcggcaa
     1561 aaggcaacac agctttggaa ccgttctcca gctggaaaaa atcgggacgt tgaatttgag
     1621 acatggctat cactccgaaa atatccgggt gacaggctca cccgggatca atccgggcgt
     1681 acaggatcgt catattgccg ggaacacagc gacgccatcg ctgcaggcgc ggaaatttta
     1741 accatgcccc ggtttgccgg caagctataa tgcgatgctt tcagtggatc gaaggcctgc
     1801 agccgatgac atcgaatacc ttcaccatcc ggccggccgc cactggcgac cacgcgcaat
     1861 ggaagccgct ctggcagggc tacctcgagt tttaccgttc gagcctggcg cccgaagtca
     1921 ccgacctgct gtggcagcgc attctcgacc cgcagcacga gatacgcggc ctgctcgccg
     1981 aggccgacgg cgggctgacc gggctggtgc acttctttcc acatgcgcat acctggaact
     2041 ccgccccggt ctgttacctg aacgacctgt tcgtgttgcc gtcggtacgc ggcggcggtg
     2101 tcggccgcgc gctgatcgag gcggtggtgg acgaggccag gcggcaggag tggacccgag
     2161 tctactggca tacgcagcat gacaacgcgg tcgcgcgctc gttgtacgac aaggttaccg
     2221 gcggcaccga cggcttcgtc aattacacct tgatccttgc caaggattca ccgcaataaa
     2281 gcccatcgcg gcattgtcgt gcggaccgat acgccggatt tctccattga taaacacatg
     2341 gcgccaaggc acacggcaag tcatcggttt gccgtgccct ttccgagaag acctccccaa
     2401 ggaccccggc gcgggcgggc cgcgggcggc gccggggtga cggggctagt ccggggtgac
     2461 ggggctagtc cggggtgatt taggctagcg gatttcgagg ccgaactggg cggcggattc
     2521 gacgaagcct tcccacagcg acagcgcgaa gccgcgcgga ataaacagct cgtagccgtc
     2581 cgccgaacgc cacagtgtga caccgacgtg gtgggtggcg gatgacgcca cgctgccgac
     2641 cgggaacgag gcgtcacgca aatccagcgg caggtgccgg ttgaggaact cggcggcggc
     2701 gtcgccgctc acccgcaagc gggtgcgcga atgcgacagg tcgagcgtca tcgcctgctc
     2761 ggcggcgaac gcgggcgcct cgatgtcgac cagccaccac ttcatcggct cgatgcgcag
     2821 catcgacccc tgcccgccgc tgaccgccgc gccgggaccg gcggtcgcgt cggcgccgat
     2881 caggccggcg agtttctcgc cgacggcatc gatcgaatcc cgccaggccg cgacctggtg
     2941 caacaccagg ccctgcatgt cctgcagaat gaccccggta cgctcgtgat caccgtgatg
     3001 tcccggccgg tagtggccgg ccagcgctga aacccgatca gccatgcatg cgttctccct
     3061 gcgggtcgta catatgcggc gacacgacct cgacatcgat gttgcccttg cgcaccgggt
     3121 cggcggcgac cagcgttttg ccctcccagg cctggtgacc gccagcgacg aagccaaggc
     3181 cgatccagtg accgagcgcc ggcgagtggg tcaccgcggt gatccagccc tcgcccaggc
     3241 ccgagacctt gccggccgcg cagaggatgg cgccggcgtt gaatttctgc gagcggtcga
     3301 ccggcttgat gccgaccagc cgcggccggt cctcgcgcag cagttcgggt cgttcgcgca
     3361 tcgccttgcc gatgtagagc ttgtccgggt tggccatctt gccgaggccg gcgtcgtcga
     3421 tgctgacccg gccgtcgagc tcggcgccgg tgacgtggcc tttctcgatg cgcatcgcgc
     3481 cgagcgcctc gagcccgtac aggcagccgt cgtgctgttg cgcctcggtc cacagcaatt
     3541 ccatcatcgc ggtcgcgtag tctgagggca cgtaagcctc gtaagccatt tcgcccgaga
     3601 agctgatgcg cgcgatgcgg cacggaatgc ctcccttcag actgctttcg atcacgccca
     3661 tgaacggcaa cacttcgttg tccataaccg aggcatcctc gacacaggct ttgagcaccg
     3721 cgcgcgattg cggtccggcg accgcgctgc cggcccattg ctcggagacg ctggtgacgt
     3781 gcacgcgcag ctcctgccag cgggtctgca gcaattcctc gagccaggcc atgaccttgc
     3841 cggcgtgcgc ggtggtggta gtcatgaagt aatcggtttc cgacagccgc caggtggtgc
     3901 cgtcgtccat gacgataccg tcgtcgcgca gcatgatgcc gtaacgcgcc ttgccgatcg
     3961 gtaacctggc gaacggattg gtatagatgc ggttgagaaa ctcggtcgcg tccgggccct
     4021 gcaccgcgat cttgcccagc gaagtcacgt cgcacagccc gaccgcggcg cgcgtggtct
     4081 cggtttcgcg cacatacgcc tcgctgatgc cctcgccctg gcgcgcaaaa taccacggcc
     4141 ggtgccacag gcccgccatg gtcatggtcg caccctgctc taggttccag tcgtgcatcg
     4201 gcgtgcgccg cagcggccgg aaatgctggt cgacgttgcg ccccttgagc gcgccgatgg
     4261 cgaccggcgt gtacggcggc ctgaaagtgg tcgtgccgac ctcggggatc tcgacaccga
     4321 gggcctcggc catcagcgcc aggccgatga tattgccgac cttgccgccg tcggtggcca
     4381 tgccgagcgt ggtgtaacgc ttcaggtgct cgaccgacac gaaaccctcg cggtgcgcga
     4441 ggcgcacgtc atcggcggtg acatcgtgct gcggatcgac gaacgccttg gtcttgcgag
     4501 ccggatcgcg gatctcgtag agcggtcgaa tcgggtgctc ccagccgccg atcgccggca
     4561 tcgctttggc gcgcgccttg ccgccgagcg ccttgaccgc ccgcgccccc gccgccgcac
     4621 ccgagtcgaa gcaggcgtca gtgttccaga tgccggcggc ggagccggcg acctggatcg
     4681 gctcgtcggt ttcgggaacg acgaaacagg cgttatcggc attccacacc ggctttac
//
LOCUS       DATCZV010000336         4239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_239131, whole genome shotgun sequence.
ACCESSION   DATCZV010000336 DATCZV010000000
VERSION     DATCZV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239131"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /gene="rnt"
                     /locus_tag="VIW27_07570"
     CDS             <1..592
                     /gene="rnt"
                     /locus_tag="VIW27_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEY5739569.1"
                     /translation="ARRFRGFLPVVVDVETGGFNEVTDALLQIAAVLLDFDDEGRLYC
                     TETIACHVTPFEGANLDPKSMQVNGIDVDHPFRLALDEKQALPKIFKPVREAIKRQGC
                     NKAILVGHNAHFDLKFINVAAARAGIKRNPFHPFSTFDTVTVAGLAYGQTVLARAVRS
                     AGMEWDTAEAHSAIYDAEMTAALFCKVVNEFAFMRP"
     gene            642..1544
                     /locus_tag="VIW27_07575"
     CDS             642..1544
                     /locus_tag="VIW27_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5739570.1"
                     /translation="MRKEDLSIVVVDDLQFSREVVKSGLTRSGFGDIRTAASADEALY
                     QLNQRRAHVVLADFWMPGMNGLEMTDLIRRWDENNDRYTGIVLLTAEDTASSIVVAFD
                     RGVDDFISKSANQFELAARVYGAGRNAFRQNQIRERMRRLSDQYLRVKRYNLLDSETN
                     LPNRAQLELHLESLLEHCSSRGGGIGLGMLAIDSARGELRIGTLKTIANSIQLALRPM
                     DMVARFSLNSFAVAVHYQDPQAFNLQLFERLVHSIRRHTLLTTDQGKQFTFSIGVWHG
                     HELQPAPKVAEIIGYANQARSPIE"
     gene            complement(1576..1902)
                     /gene="grxD"
                     /locus_tag="VIW27_07580"
     CDS             complement(1576..1902)
                     /gene="grxD"
                     /locus_tag="VIW27_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY5739571.1"
                     /translation="MDVLERIKQQVETYPVLIYMKGTPQLPQCGFSSRTAEALKALDI
                     EFAYVNVLADPEIFENLPRFADWPTFPQVYVDGELIGGCDITLEMYESGELQKVTSAA
                     LEKQAS"
     gene            complement(2235..4016)
                     /locus_tag="VIW27_07585"
     CDS             complement(2235..4016)
                     /locus_tag="VIW27_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HEY5739572.1"
                     /translation="MCGLFGALSFESKTIPEAHASAMSERVARRGPDDRGEYYDGPVM
                     LGHRRLSIIDLSPAGHQPMADASGRYCIVFNGTIYNYPQLREQLIGLGYAFNSHSDTE
                     VIVNAYAHWGEQCVERLHGMFAFAIWDRERRDLFLARDRMGIKPLYYARDRAGFYFAS
                     NPQALLATGLFPTELDAVGLHHQLSLHAVIPAPRTLIKSIRKCRPAHSMTVTAGGEID
                     ERRYWTLVAKRPAERLSAEDWNLAIHDSLMQAVKKRLTIADVPVGVLLSGGLDSSLLV
                     ALLDEAGQHDIRTFSIGFEDQPEERGNEFEFSDPVAERYATDHQKFLVPNDEVLTRLP
                     EAVEAMAEPMFGQDAVAFYLLSEQVSKSVKVVQSGQGADEVFAGYFWYAQMWAAQDEA
                     PLPRFASRYVDRDHAEFLDTVSSAYRGDDYTSQLIEENLDSGEADSFLDAVLRFDVTT
                     LIVDDPVKRVDNMTMAWGLEARVPFLDHQLVELAASCPPELKLKHDGKGILKEIARGR
                     IPDAVIDRPKGYFPMPALKYVRGDFYQFMADTLNSRASRDRGLFERAYIDRLLAEPEQ
                     HFTRLNGSKLWHCALLEYWLQKHLDNR"
BASE COUNT          797 a         1347 c         1298 g          797 t
ORIGIN      
        1 ggcgcggcgc ttcaggggtt tcctgccggt ggtggtcgac gtcgaaaccg ggggcttcaa
       61 cgaggtgacc gacgcactgc tgcagatcgc cgcggtgttg ctcgacttcg acgacgaggg
      121 ccggctctac tgcacggaga cgatcgcctg tcacgtgacg ccgttcgagg gcgccaacct
      181 cgatcccaaa tcgatgcagg tcaacggcat cgacgtcgat cacccgtttc gcctggcgct
      241 ggacgaaaaa caggcactgc cgaagatttt caaaccggta cgcgaggcga tcaagcgaca
      301 gggctgcaac aaggcgatcc tggtcggcca caacgcgcat ttcgatctca agttcatcaa
      361 tgtcgccgcc gcgcgcgccg gcatcaagcg caacccgttt catcccttca gtaccttcga
      421 cacggtgacc gtcgccggcc tcgcctacgg gcagacggtg ctggcgcggg cggtgcgatc
      481 cgcgggcatg gagtgggata ccgcggaggc gcactcggcg atctacgacg ccgagatgac
      541 cgcggcgctg ttttgcaagg tcgtcaacga gttcgctttt atgcggcctt gaacgaagtt
      601 ttactttata ctcactgcca ctcgagttcc taccaccgac cttgcgcaaa gaagacctat
      661 ccatagtcgt cgtcgacgat ctccagttca gccgcgaggt ggtcaagtcc gggctcaccc
      721 gctccggctt cggcgatatc cgcaccgccg cgtcggccga cgaagcgctc taccaactca
      781 accagcggcg cgcacacgtg gtgctggcgg atttctggat gccgggcatg aacggactgg
      841 agatgaccga cctgattcgg cgctgggacg agaacaacga ccgctatacc ggcatcgtgc
      901 tgctaactgc cgaggatacc gcgtcgagca tcgtggtcgc ctttgatcgc ggcgtcgacg
      961 atttcatttc caagtcggcc aaccagttcg agctcgcggc ccgcgtgtat ggcgccgggc
     1021 gcaatgcttt ccggcagaac cagatccgcg agcgcatgcg ccggctcagc gaccagtacc
     1081 tgcgcgtcaa gcgttacaac ttgctcgata gcgagaccaa cctgccaaac cgcgcgcaac
     1141 tcgagctgca cctcgaatcc ctgctcgagc attgcagctc gcgcggcggc ggcatcggtc
     1201 tcggcatgct ggcgatcgat tcggcgcgcg gcgagttgcg catcggcacg ctgaagacca
     1261 tcgccaattc gatccagtta gcgctgcggc cgatggacat ggtggcgcgc ttcagcctca
     1321 acagcttcgc ggtcgcggta cactaccagg acccgcaagc ctttaacctg cagctgttcg
     1381 agcgcctggt ccacagcatc cggcggcata cgctgctgac caccgatcag ggcaagcagt
     1441 ttactttcag tatcggcgtc tggcacggcc acgagctgca gcccgcaccg aaggtggcgg
     1501 aaatcatcgg ctacgcaaac caggcccgca gcccgatcga gtaaccgccg cgcggcgcgg
     1561 tgaaaacggt ggtggtcagc tggcctgctt ctccagcgcg gcgctggtga ctttctgcag
     1621 ttcgcccgat tcgtacattt ccagcgtgat atcgcagccg ccgatcagtt cgccgtcgac
     1681 atagacttgc ggaaaggtcg gccagtcggc aaagcgcggc aggttctcga atatttccgg
     1741 gtcggcgagc acgttgacat aggcgaactc gatgtcgagt gccttcaggg cttccgcggt
     1801 acggctggaa aatccgcact gcggcaattg cggcgtgccc ttcatataga tcagcaccgg
     1861 ataggtttcg acttgttgct tgatacgttc caggacgtcc attgatacct ctgacaataa
     1921 ccaagtaaat tactaaggta ttatcataac cgttttgttc aagcgatgta aatcgaagac
     1981 tcgacagcgg cgagctcccg ctcgcccggt gcatgcaaat cgccgcgtgc cggcacggcc
     2041 ccgggacggc agccatcgcc ccggcaggat tcccgccggc catggccggg gcccggcgag
     2101 gcgccgaccg cagcagccgt cagcgctcgg gtgaccgtca ccggccgcgc cgtcggcgac
     2161 ggccgtggcg ggcctgcatc accggcatcg gcggttagcg atgcgtcgat gttgcggcgc
     2221 gagcaatccg cgggctagcg gttatccagg tgtttttgca gccagtattc gagcaatgcg
     2281 cagtgccaca gcttgctgcc gttgaggcgc gtgaaatgct gctcgggctc ggccagcagg
     2341 cgatcgatat aggcacgctc gaacaggccg cgatcgcggc tcgcgcgact attgagcgta
     2401 tcggccataa actggtagaa atcgccgcgc acgtacttca gcgccggcat cgggaaatag
     2461 cccttgggac gatcgatgac cgcgtcggga atgcggccgc gtgcgatctc cttgaggata
     2521 cccttgccat cgtgtttcag tttcagctcc gggggacacg atgccgcgag ttccaccagc
     2581 tggtgatcga ggaacggcac ccgcgcttcc agcccccagg ccatggtcat gttgtcgaca
     2641 cgcttgaccg gatcgtcgac gatcagcgtg gtgacgtcga accgcagcac cgcgtcgagg
     2701 aaactgtcgg cttcgccgct gtcgaggttt tcctcgatca gctgcgaggt atagtcatcg
     2761 ccgcgataag cgctgctgac ggtgtcgagg aattccgcgt gatcgcggtc gacatagcgg
     2821 ctggcaaagc gcggcaacgg cgcctcgtcc tgcgcggccc acatctgcgc ataccagaaa
     2881 tacccggcga aaacttcgtc ggcgccctgc ccgctctgca ctaccttgac ggatttcgaa
     2941 acctgctcgg acaacaggta gaaggcgacc gcgtcctgac cgaacatggg ttcggccatc
     3001 gcctcgacgg cttccggcag gcgcgtcagc acctcgtcgt tgggtaccag gaatttctgg
     3061 tggtcggtgg catagcgctc ggccaccggg tcggaaaact cgaactcatt gccgcgctcc
     3121 tcgggctggt cctcgaaacc gatgctgaag gtgcgaatat cgtgttgccc ggcttcgtcg
     3181 agcaacgcca ccagcaggct cgaatcgagc ccgccggaga gcagcacgcc gaccggcacg
     3241 tcggcgatgg tcaatctttt cttcaccgcc tgcatcaacg agtcgtgtat tgcaaggttc
     3301 cagtcctcgg ccgacaggcg ctcggcagga cgtttcgcga ccagcgtcca gtagcgccgc
     3361 tcgtcaattt cgccgccggc ggtcaccgtc atgctgtggg cgggacggca cttgcggatc
     3421 gacttgatca gcgtgcgtgg tgccgggatc actgcatgca aactcaactg gtgatgcagc
     3481 ccgaccgcgt cgagctcggt cgggaacagg ccggtcgcca gcagcgcctg cgggttcgac
     3541 gcaaaataaa agccggcccg gtcccgggcg tagtacagcg gcttgatacc catgcgatca
     3601 cgcgccagga acaggtcgcg gcgctcgcga tcccagatcg cgaacgcgaa cataccgtgc
     3661 agccgctcga cgcattgttc accccagtgc gcataggcat tgacgatgac ttcggtgtcg
     3721 ctgtgcgagt tgaacgcgta acccaggccg atcaactgct cgcgcaattg cggatagttg
     3781 taaatggtac cgttgaagac gatgcagtaa cggccgctgg catccgccat cggctggtgc
     3841 cccgccggcg acaggtcgat gatcgacagc cggcggtgac cgagcatcac cggtccgtcg
     3901 tagtactcgc cgcggtcgtc ggggcccctt ctcgcgaccc gctccgacat ggcgctcgca
     3961 tgcgcctcgg ggatggtttt tgactcgaaa ctgagcgcgc cgaacaagcc gcacatatga
     4021 ctttacccca gtgttatcga tgctcgtcga gcgcccgccc gcgaacggat cgccgcattg
     4081 cgccctatac cgaaagtcct tatactgcgc cgcgtgcaac gggcatgacc agactgctat
     4141 gtcgcgcccc gtattctaaa cgtttcttgt ggggagtcat caatgaatcc gttaaaatcg
     4201 attccgggta cgatcatatc cggctttgtg ctggccatc
//
LOCUS       DATCZV010000337         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_768981, whole genome shotgun sequence.
ACCESSION   DATCZV010000337 DATCZV010000000
VERSION     DATCZV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768981"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VIW27_07590"
     CDS             complement(<1..182)
                     /locus_tag="VIW27_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5739573.1"
                     /translation="MPEAGRKDLSKGTLFVVATPIGNLGDLSPRAREVLEQVDLVAAE
                     DTRVTGRLLSHFGIGTT"
     gene            294..2177
                     /locus_tag="VIW27_07595"
     CDS             294..2177
                     /locus_tag="VIW27_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY5739574.1"
                     /translation="MAIVIAGCATPGGISGGGQAERRAESLSQSGQYSDAAGVYIGLA
                     ASASGAERDRLTLLAVEQWLFAGDGRRARNALSQVPTPDGGELLWLWSADAAALSLWE
                     GRPDRALALLEPLSRQALPASHRARVEALRADAWFQKGEPLRAVDLYVQRENWLDSPR
                     AVEAGRSRLWAGLQVVDVDALRQAATVAYDPIARGWLSLAILANSTGQQGIGWGNGIV
                     RWQETYAGHPATSVLSDLTLPEDSLLNYPRQIALLLPLSGNGEAAGNAIKNGFLGSYF
                     SAVGGIEHQQQLRIYDVADGGASAVYQRAVLDGAEFVVGPLLRRNVTELAGELLLPVP
                     VLSLNYLPNNVPAPPGFYQFALAPEDEAAAAAQRAIGDEGLTAVALVPANDWGRRVLG
                     SFATELEGLGGTLLDVQNYEPTAQDFSFEIENLMGLSQSVQRYQRLRANIGGPLQFDP
                     RRRQDIDFIFLGADSRAGRLIKSQLKFHYSGDLPVYSTSFIYAMDGRSDADLDNVRFA
                     DAPWVISPPAWIADYPALYGQYWPSERRLARLHAMGYDAYHLVGALFNARETGIEEIV
                     GATGRLFMTADGRIHRKLAWAQFERGQPVPLPESNEFQDLLDDPDAVEEANFEWPEPS
                     PDD"
     gene            2131..>2524
                     /locus_tag="VIW27_07600"
     CDS             2131..>2524
                     /locus_tag="VIW27_07600"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY5739575.1"
                     /translation="MRKRISNGRSRHPTTRKRLGRRAEQRALAYLQRRGLRLVTRNYR
                     CRFGEIDLIMRDNDCIVFVEVRFRTSISFAPPALTVDTFKQSRIEKTAQSYLARHPRH
                     ADHPARFDVIAIDSADGAAGTIQWIRDAF"
BASE COUNT          467 a          797 c          794 g          466 t
ORIGIN      
        1 gtcgtgccga tcccgaaatg agatagtaat cgaccagtga cgcgcgtatc ctccgcggcg
       61 accaggtcga cctgctccag cacttccctg gcgcgcggcg acaggtcacc gagattgcca
      121 ataggcgtcg caacgacgaa taatgtgccc ttactcaagt ctttccttcc ggcctcggga
      181 atattcacgt gatcgaaact gacaaccgac atcctgcacg gcacgccggt cctcaggcaa
      241 gcagcaggcc ggcgtggatt cggaatatgc aacgcttcac gatagccgcg gtcatggcca
      301 ttgtcatcgc cggctgcgcg accccgggtg gcatatccgg cggcggccag gccgagcgcc
      361 gcgcggaatc cctctcgcag tcaggtcagt atagtgacgc ggccggcgtc tacatcggcc
      421 tggcggcgag cgccagcggg gcggagcgcg atcggctgac gctcctcgct gtcgagcaat
      481 ggctgtttgc cggcgatggc cgcagggcgc gcaacgcgct gagccaggtt ccgacgcccg
      541 atggcggcga actcctgtgg ctttggagtg ccgacgccgc agcattgtca ctttgggaag
      601 gccgccctga tcgggcgctg gcgttactcg aaccgctgtc gcggcaggcg ttgcccgcca
      661 gccaccgcgc tcgcgtcgag gcgttacgcg ccgacgcctg gttccagaaa ggtgagccgc
      721 tgcgcgccgt cgacctgtat gtacaaaggg agaactggct ggacagcccg cgcgccgtcg
      781 aagcaggcag gagcaggctg tgggcgggat tgcaggtcgt cgatgtcgac gcgttgcggc
      841 aggcagcaac cgtcgcctat gacccgatcg cacgtggctg gctgtcgctg gcgatcctcg
      901 ccaattcgac cggccagcag gggattggct ggggcaacgg cattgtccgc tggcaggaaa
      961 cttatgccgg tcacccggcg accagcgtcc tcagcgacct gactttgccg gaagacagtc
     1021 tgctgaacta tccgcggcag attgcgctgc tcctgcccct gtcaggcaac ggtgaagccg
     1081 cgggaaacgc catcaagaat ggctttcttg gatcctattt ttccgcggtc ggaggcatcg
     1141 aacatcagca acagttgcgg atatacgacg tcgccgacgg cggcgccagc gccgtctatc
     1201 agcgagcggt gctcgacggt gcggaattcg tcgtcggccc tctgctgcga cgcaatgtta
     1261 ccgaactggc cggtgaactg cttttgccgg tcccggtgct gagtctcaac tacctgccga
     1321 acaatgtgcc cgcgccaccc ggcttctacc agttcgccct ggccccggaa gacgaggcgg
     1381 ccgctgccgc gcagcgcgcg atcggcgatg aaggcctgac ggccgtcgcg ctcgttcccg
     1441 ccaacgactg gggacgtcgc gtgctgggaa gctttgcgac ggagctcgaa gggctcggcg
     1501 gcacgttgct cgatgtccag aactatgagc cgactgcgca ggacttctcc ttcgagatcg
     1561 aaaacctgat gggactcagc cagagcgtgc agcgctatca gcgtctgcgc gccaacatcg
     1621 gcgggccgtt gcagttcgac ccgcgacgcc ggcaggacat cgacttcatt ttcctgggtg
     1681 ccgattcgcg cgccggccgc ctgatcaaat cccagctcaa gtttcactat tcgggtgatc
     1741 tgcccgttta ctcgacttcc tttatctacg ccatggatgg acgatcagat gcagatctcg
     1801 acaacgtcag gtttgccgat gcgccatggg tgatttcgcc gccagcctgg atcgccgact
     1861 acccggcgct ctacgggcag tactggccgt ccgagcggcg cctggcacga ctgcatgcca
     1921 tgggttacga cgcgtatcac ctggtaggcg ccctgttcaa tgcgcgtgaa acgggcatcg
     1981 aggaaatcgt cggtgcgacc ggccgccttt tcatgacggc ggatggccgc atacaccgca
     2041 agctggcgtg ggcgcagttc gagcgagggc aaccggtgcc gttaccggaa agcaacgaat
     2101 tccaggattt gctggacgat cccgacgcag ttgaggaagc gaatttcgaa tggccggagc
     2161 cgtcacccga cgactagaaa acgcctcggc aggcgtgccg aacaacgcgc gcttgcctac
     2221 ctgcaacgcc gcgggctgcg gctcgtcacg cgcaattacc ggtgtcgctt cggcgagatt
     2281 gatctgatca tgcgcgacaa cgattgcatt gtttttgtcg aggttcgctt ccggacctcg
     2341 atatcattcg cgccaccggc attgactgta gacacattca agcaatcgag gatcgaaaaa
     2401 actgcgcagt catacctcgc gagacatcct cgacacgccg atcacccggc aagattcgac
     2461 gtgattgcga tagatagcgc cgatggcgct gccggcacga tacaatggat acgcgatgcg
     2521 tttc
//
LOCUS       DATCZV010000338         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_397795, whole genome shotgun sequence.
ACCESSION   DATCZV010000338 DATCZV010000000
VERSION     DATCZV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397795"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VIW27_07605"
     CDS             <1..1025
                     /locus_tag="VIW27_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739576.1"
                     /translation="YLIGSLLTLPITLNFAHLERLLQRRWVVTLGVGFTLLAMTILLF
                     ADGMIVALAIGLQQASASLFSVCLSLYIMDYIGRQQLIYTETRRMLYTGIVWLIGPTL
                     GLWLWDSGPRWSPFVLTACGALGMLGYFWYLRLGSDPAIRRARTHSASIFRISPRYFE
                     QRALRIAYWITMSRSIFWVTLFVYGPIYTVEAGLPTWVAGGLLSLASALLMASPLVRR
                     LAGRWGTRRLIVGALLLTGGSMLMLFVLGAPRPLGLLFWMTGAGGGMMLDVLGNIPFM
                     RMVRARERTEMTMVFSTWREGSQLLTPLLVSLVLLVAPFKAYYALLAALLFAAAFTAS
                     WLPRRL"
     gene            complement(1044..>2282)
                     /locus_tag="VIW27_07610"
     CDS             complement(1044..>2282)
                     /locus_tag="VIW27_07610"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEY5739577.1"
                     /translation="ETTVVWDRASGEPVYRAIVWQDRRTAEYCETLRAGDHEAMVAGK
                     TGLLLDPYFSGTKVNWILDNVDGARARAARGELAFGTIDCFLIWRLTGGERHVTDASN
                     ASRTLLFNIHEQRWDPQLLEMLEVPDSLLPEVLDCAADFGATRKDLLGQAIPICGVAG
                     DQQAALIGQACFQPGMIKSTYGTGCFMVLNTGSEAVQSSNKLLTTVGYRLNGETTYAL
                     EGSIFVAGAAVQWLRDGIRIIESAPQTEAMAAGLDSNHGVYLVPAFTGLGAPHWDPDA
                     RGAIFGITRDTGPAQLARAALESVAYQTFDLLEAKRRDGLEAKRLRIDGGMVRNDWLC
                     GFLADLLGIVVERPVQTETTALGAAYLAGLQVGMFASLEDIAAHWQADREFEPRMEDT
                     QRNLLLAAWHDAVIKVRTNA"
BASE COUNT          328 a          744 c          781 g          429 t
ORIGIN      
        1 cctacctgat cggatcgctg ctgacgctcc cgatcactct caacttcgcc cacctcgagc
       61 gcctgctaca gcgtcgctgg gtggtcacgc tgggcgtcgg cttcacgctg ctggcgatga
      121 ccatcctgct gttcgccgac ggcatgatcg tggcgctggc gatcggcctg caacaggcct
      181 cggcttcgct gttctcggtc tgcctgtcgc tatacatcat ggattacatc ggccgccagc
      241 aactgatcta caccgagacc cggcgcatgt tgtacaccgg tatcgtctgg ttgatcgggc
      301 cgacgctcgg gctgtggctg tgggacagcg ggccgcggtg gtcgccgttc gtgctcaccg
      361 cctgcggtgc gctcggcatg ctcggctact tctggtacct gcggctcggc agcgatccgg
      421 cgatccgcag ggcgaggacg cacagcgcca gcatatttcg catcagtccc cgctacttcg
      481 aacagcgcgc gttgcgcatc gcctactgga tcacgatgtc gcgctcgatc ttctgggtca
      541 cgctattcgt ctacggtccg atctacacgg tcgaggcggg gctgccgacc tgggtcgccg
      601 gcggcctgtt gtcgctggcc agcgcgctgc tgatggccag cccgctggta cgaagactgg
      661 ccggtcgttg ggggacgcgg cgcctgatcg tcggcgcgct gctgctgacc ggcggttcga
      721 tgttgatgct gttcgtgctc ggcgcgccgc ggccgctggg cctgctattc tggatgaccg
      781 gggccggcgg cggaatgatg ctcgacgtgc tcggcaatat accgttcatg cgcatggtca
      841 gggcgcgcga acgcaccgag atgacgatgg tgttctcgac ctggcgagaa ggctcacagc
      901 tgctgacgcc gttgctggtc tcgctggtgc tgctggtcgc gccgttcaag gcgtactacg
      961 cactgctggc ggcgttgctg ttcgcggccg cattcaccgc ctcgtggctg ccgcggcgcc
     1021 tgtagataac ggccgcgttg cgctcaggcg ttggtgcgca ccttgatcac cgcatcgtgc
     1081 caggccgcca gcaacaggtt gcgctgcgtg tcttccattc gcggctcgaa ctcgcgatcg
     1141 gcctgccagt gcgcggcgat gtcctcgagc gacgcgaaca ttccaacctg caggccggcg
     1201 aggtaagccg cgccgagcgc cgtggtctcg gtctgcaccg ggcgctcgac gacgatgccg
     1261 agcaggtcgg cgagaaaacc gcacagccag tcgttgcgca ccataccgcc atcgatgcgc
     1321 agccgtttcg cctcgaggcc gtcgcgacgc ttggcctcga gcaggtcgaa agtctggtaa
     1381 gccaccgatt cgagcgcggc gcgtgccagc tgcgccggac cggtgtcgcg ggtgatgccg
     1441 aagatcgcgc cgcgtgcgtc cgggtcccag tgtggtgcgc cgaggccggt gaacgcgggc
     1501 accaggtaga ctccgtgatt cgagtcgagg ccggcagcca tggcctcggt ttgcggcgcg
     1561 gactcgatga tgcgaatacc gtcgcgcagc cattgcacgg cggcgccggc gacgaagatc
     1621 gaaccttcga gcgcataggt cgtctcgcca ttcaggcggt agccgaccgt ggtcagcagc
     1681 ttgttgctcg actgcaccgc ttcgctgccg gtgttcagca ccatgaaaca gccggtgccg
     1741 taggtgctct tgatcatccc cggctggaag caggcctggc cgatcagtgc cgcctgctgg
     1801 tcgccggcga cgccacagat cgggatcgcc tggccgagca ggtccttgcg ggtggcgccg
     1861 aagtccgccg cgcagtcgag cacttccggc aacagcgaat cgggtacctc gagcatctcg
     1921 agcagttgcg ggtcccagcg ctgttcgtgg atgttgaaca gcagcgtgcg cgacgcgttg
     1981 ctggcgtcgg tgacatggcg ctcgccgccg gtcaggcgcc agatcaggaa acagtcgatg
     2041 gtaccgaagg cgagctcgcc gcgggccgcg cgcgcgcgcg cgccgtcgac gttgtcgaga
     2101 atccagttga ccttggtgcc ggaaaaatag ggatcgagca gcagcccggt cttgccggcg
     2161 accattgctt cgtggtcgcc ggcgcgcagc gtttcgcagt attcggcggt gcgccggtcc
     2221 tgccagacga tggcgcggta aaccggctcg ccgctggcgc gatcccagac cacggtggtt
     2281 tc
//
LOCUS       DATCZV010000339         4801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_397820, whole genome shotgun sequence.
ACCESSION   DATCZV010000339 DATCZV010000000
VERSION     DATCZV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397820"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..561)
                     /locus_tag="VIW27_07615"
     CDS             complement(214..561)
                     /locus_tag="VIW27_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739578.1"
                     /translation="MRRSQRKIIAWLLLIAVSLNPLVGVHALNAGPVPAIADCQIDLA
                     ASAASEPADGAGPITDCARHHACATSCQFAPLQPSGPQQPRVERRPWPALSGGPENIV
                     TRFLESIERPPRV"
     gene            complement(843..989)
                     /locus_tag="VIW27_07620"
     CDS             complement(843..989)
                     /locus_tag="VIW27_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739579.1"
                     /translation="MAESVCLALLFGRDRSRRNRDQIANESDDFRLPDIVMSADQLLV
                     DLDI"
     gene            1350..2096
                     /locus_tag="VIW27_07625"
     CDS             1350..2096
                     /locus_tag="VIW27_07625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5739580.1"
                     /translation="MRISTGILFFCLALFLPPLQAGHDSGAQVKIDEVAAGEGIEAMP
                     FSIVEVHYTGRLTDGTVFDSSVGRGEPFRFTLGTGQVIPGWELGIQGMKKGGKRVLKI
                     PPELAYGARGAGNVIPPNATLEFEVELVSVTPPPFESIDSVQLEEKLASGIKLIDVRR
                     PEEWQQTGVVEGSIKSTAFDANGKFLASFVEMLEKTVEPGEEFALICRTGNRTATLTN
                     YLVTQGGYSNVLNVRDGITKWIGEGRPVSK"
     gene            2533..3237
                     /locus_tag="VIW27_07630"
     CDS             2533..3237
                     /locus_tag="VIW27_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5739581.1"
                     /translation="MGKNVDEAADPVEILQPMDLFRELDRREREQVSKLIKWRGYRKN
                     TEVVHHLGRDDDVYFIAVGRVRVTIFSAEGKEISYQELGVGNMFGELSAIDGLPRTAS
                     VITLEDSRIGVLGRAAFWELLQQYPVLASGIMKRLAGMVRFLTDRVYQYGALDVNDRV
                     RMEILRLARQNLQDDNSASIEDFPTHREIANRVNTHREAVTKVLNELKRLGIIRQDKR
                     VLTVSDLSRLVRLLPE"
     gene            3776..4468
                     /locus_tag="VIW27_07635"
     CDS             3776..4468
                     /locus_tag="VIW27_07635"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5739582.1"
                     /translation="MKYLVSYLGQDRALVANTSHACAADQVVAGYCSADSCDEEELQG
                     PAGILYADIVDYSRLSEHREIGVRARLVDSMEHFRAVITANSGRLAHFAGDAILAEFN
                     DVDSALGCAIKVQLASRQWNADLDLPRQVRFRIGVSFGEVIATRGNVYHDAVKLAARL
                     ESLACAGSICVSKNVRSISSAGSTYRFVGTGKRYLKHAGKPVETFWILIGSEQITELA
                     MHGTAGLPDRAS"
BASE COUNT          919 a         1392 c         1541 g          949 t
ORIGIN      
        1 cgaagcggcg gatagtgttc aaagtctcgt tacgcattta aagttcctgt tttcaagcgc
       61 tcgggcctcg gcagtcatgc gccacgtttg accggttgag ctcgggcggc ggtaatcggg
      121 aattgcgtcg aaacggcgat ctgccagtcc gtgggccggt ttacacgcca ggtgttccgg
      181 attcgaggag tctcgccgcc ggcgcgatcg aggttaaacg cgcggcggtc gctcgatact
      241 ttcgaggaag cgggtaacga tattctccgg cccgccggaa agcgccggcc acggccgccg
      301 ctcgacccgc ggctgctgcg gcccggacgg ctgtaaaggc gcaaactgac aggaggtggc
      361 acaggcatgg tgtcgcgcgc agtctgttat cgggccggca ccgtcggcag gttcgcttgc
      421 ggcactggcc gccagatcga tctggcagtc cgcgattgcc gggaccggtc ccgcgttcag
      481 cgcgtgcacg ccgacgaggg ggttcaggct aaccgcgatc agcagcagcc aggcgattat
      541 tttacgctgc gaacggcgca tcagcgatcc acgccgccgg tcggggcagc ggcccgaggc
      601 cgaacctgat ggcttgacgc attcatgcct gcggataaac cgtcgtctgt ggacagtgtc
      661 tgaagtgtgg catcgcgact gccatgataa ttgatttggc tcagtaaatc gatgattcgc
      721 atcgggcggc aaatggcagt gtagtgggca tgcgaatctc cgtctcgcgc gcgcgtcgag
      781 gtgccgaggg agacggcggc cggctcgagt cgctgcagcg aaccaccgcg ctgaattcct
      841 aattaaatat ccagatcgac aagcagctga tcggcactca taacgatatc tggcagacgg
      901 aaatcatcgc tttcgttcgc gatctgatcg cgatttcgac gacttcgatc tcggccgaaa
      961 agcagcgcga ggcacaccga ttcggccatc tcgcgcttcg cggcgagaga aggcgggcct
     1021 gcatccgcgt ggccggcccc tgccgaatcc ccgcggcttg ggtgcttttt gtttgcggta
     1081 gtgatgtcgt gccccggttc gctcccggcg ctacttgtca ttcaacgcca aagcggggat
     1141 cccataacgg cgccccgctg caacatcgtg atgccccgga ccggtgccga ggtgtgagtg
     1201 tctcgaggtc gagcgaatga catcggtagc ggaatgactc tcgggccgaa gccggctatc
     1261 gccgcgtttg cactgcgtcg gcgcttcggt gtatggtcgc atcgtttgac caggtcgaac
     1321 cccgatatga aatactactg gagcccaata tgcgaatatc gacaggaatt cttttctttt
     1381 gcctggcact gttcttgccg ccgctgcagg ccggccacga cagcggggcg caggtgaaaa
     1441 tcgacgaggt cgctgccggc gagggcatcg aggcgatgcc gttctccatc gtcgaggtgc
     1501 actacacggg gcggctcacg gacggcacgg tgttcgattc gagcgtcggg cgcggtgaac
     1561 cgtttcgctt caccctcggc acggggcaag tcatccccgg ttgggaactc ggcattcaag
     1621 gcatgaaaaa gggcggcaag cgcgtgctga agatcccgcc agagctggcc tacggcgcgc
     1681 gcggcgcggg caacgtcatt ccgccgaacg cgacgctgga attcgaggtc gagctggtgt
     1741 cggtcacgcc gccgccgttc gagagcatcg acagtgtcca gctggaggag aaactggcca
     1801 gtggtatcaa gctgatcgac gtccgccggc cggaggaatg gcagcaaacc ggggtcgtcg
     1861 agggcagtat caagtcgacg gcgttcgacg ccaacggcaa gtttctcgct tcgttcgtcg
     1921 aaatgctcga aaaaaccgtc gagcccgggg aggaattcgc gctcatctgc cgtaccggca
     1981 accgcaccgc gacgctgacc aactacctgg ttacccaggg tggttactcg aacgtactca
     2041 acgtccgcga cggcatcacc aagtggatcg gcgaaggccg cccggtcagc aagtaaggca
     2101 actgaggcgt tcgtgcccgg cggcggtgcg ccgggcagcg cgataacact atcagtcgaa
     2161 tcggcggcga aaggcatcaa gccgggccgc cgatttcgtt tgccgccacg gagccgaagc
     2221 cgctcaatgc ggttccgaag tccttctcgt atccccgcag ccattcaccg tttacctgcg
     2281 gctgtcgccc tggcgccgat attgtgaggc ttcccgcggg gtcgcgggcg gcttgtacgg
     2341 gattggccgc cgttatctgc caggctccag gcccccgcgg ctaagtgctt gcacctgctg
     2401 gtagccgctt cgctccgtgc aagcgccatt cccggtcgag cctgtcgcgc tgaaggccgg
     2461 ctggatgcga gcactagaga aatttcacgg caatattgtt attctttttt gcctgtttcg
     2521 agggaaaaca gcatgggcaa aaacgtggac gaagcagccg atccggttga gatattgcag
     2581 ccaatggatt tgtttcggga actggaccgg cgtgagcggg aacaggtctc aaagctgatc
     2641 aagtggcgag ggtaccgaaa aaacaccgag gtggtgcacc atctgggccg cgatgacgac
     2701 gtgtatttca tcgcggtcgg cagggtcagg gtgactattt tttccgccga gggaaaggag
     2761 atttcctacc aggagctggg tgtcggtaac atgtttggcg aattgtcggc gatagacggt
     2821 ctgccgcgca ctgccagcgt catcacgctg gaggactcgc gaatcggtgt cctcggcagg
     2881 gcggcctttt gggagttatt gcagcaatat cccgtgcttg ccagcggcat catgaagagg
     2941 ctggccggca tggtgcgttt cctgaccgat cgggtctacc agtatggcgc gctggatgtc
     3001 aacgaccggg tgcgcatgga aatcctgcgt ctggccaggc agaacctgca agacgataac
     3061 agcgcctcga tcgaggattt tcccacccac agggaaatcg ccaatcgggt caatactcac
     3121 cgggaagcgg tcaccaaggt attgaacgaa ctcaagcgcc ttggaattat tcggcaggat
     3181 aagcgcgtgt tgacggtttc ggacctgtcg aggctggtgc gactattgcc cgagtgaatc
     3241 cggtgaccgc gggattttcg ccagcgaccg gttgattcga ctacccgcgg catcgatccc
     3301 ggtcgccgcc cggcttgatc accgcggctg cgcggtggca gcgcccgccg gcgtgcacgg
     3361 acggtgaata ttcggccggc gtctgcgggc tcggatactg aactacttcg attggcgagc
     3421 tgtgggcgga tttcgtcgcg cttttcggtt tgccgatccc aatatcgact tactgatagt
     3481 gcccggatct taaatttatt ttcgccgaat ctcctcgtcg atcgcgccgt ggcgtttcga
     3541 cccggcgttg cgctgcggtg atcggcggcg aagactcctc gcgggtgtgg gaaataccac
     3601 atacggcgct cgcccaatct gcacaattac aagggtcgaa tgcagatcga gcggcccaat
     3661 aacaatgatc cagtgtccag caatcgaaag gcaaggcgag ccgggcaacg ggtcgggcgc
     3721 cgctgaatgc agtcttaaac cggacttgag ggcgggtaat ccgatggaga cgttaatgaa
     3781 atacctggtt tcctacctgg gacaggacag ggcgctggtg gcgaacactt cacatgcctg
     3841 tgcggctgat caggttgttg ccggttactg ttcggcggat agttgcgatg aagaggagct
     3901 gcaagggcct gctggcatcc tctatgccga tatagtcgat tactcgcgcc tcagcgaaca
     3961 ccgggaaatc ggcgtccgcg ctcgccttgt cgatagcatg gaacacttca gggcggtgat
     4021 caccgctaat agcggacggc tcgctcactt cgccggcgac gccatcctgg ccgaattcaa
     4081 cgatgtcgac agcgcgctgg gttgcgcgat caaggtgcaa ctggcgtccc gacagtggaa
     4141 tgccgacctc gaccttccgc gccaggtcag atttcgcatc ggcgtgagtt tcggcgaagt
     4201 cattgccact cggggaaacg tctatcacga cgccgtgaag ctggccgcac gcctcgagag
     4261 cctggcctgt gcagggagta tctgcgtatc gaaaaatgta cgctccattt ccagcgctgg
     4321 ctccacttac cggtttgtcg gtaccggcaa gcgatacctc aagcacgccg gcaagccggt
     4381 ggaaaccttc tggatcctga tcggcagtga gcagattacc gaactggcca tgcacggtac
     4441 ggccggatta ccggatcggg cttcatgagt ggtctcctcc ggttaccggg atcgcccttg
     4501 ctcatcgggg cgttcccggt tttttttatc gacgcaggga aggggcggat accatcgatc
     4561 tatagtcccg tacaggcggg acgaacagcg cgccgggaat agagccggcg cgacgatgat
     4621 cccgcagagg tcactccccg ccagtcgggc ccacgcagac actgccggtc tgccactttc
     4681 gccgaagtgg ctggaatagc cggaacggca tcggtggcag gtaattctgg ccccggccaa
     4741 ataccggggc cagcaagacc gcgctgctgc tagtgagcct tgctgatggc gagatcgtgg
     4801 t
//
LOCUS       DATCZV010000340         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_609828, whole genome shotgun sequence.
ACCESSION   DATCZV010000340 DATCZV010000000
VERSION     DATCZV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609828"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VIW27_07640"
     CDS             complement(<1..394)
                     /locus_tag="VIW27_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5739583.1"
                     /translation="MPDTSLLKAAAERGAPPQDRKQLRRIDWLQCALEVFVSEGIDAV
                     RITRLADDLGVTRGSFYWHFENREDLIDALVSYWKDKNTAALTRSVNNAASLAEGIFR
                     FFETCIDAADFDSRLDLAIREWSRRSSRI"
     gene            464..817
                     /locus_tag="VIW27_07645"
     CDS             464..817
                     /locus_tag="VIW27_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutQ"
                     /protein_id="HEY5739584.1"
                     /translation="MTNRKAATRLMRFDDLEFAPRFEYGEMAQVAGVCGAEDGTRLGA
                     GWGRMTNAHIPWTIKYDEVLTVFEGVLRLHANGEVHELRPRDCIWLPQGTELIYEAEY
                     ALVHFAIHPSNWHED"
     gene            821..2146
                     /locus_tag="VIW27_07650"
     CDS             821..2146
                     /locus_tag="VIW27_07650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5739585.1"
                     /translation="MNTRSVTHLPRDDNTNGWSHILAPRTPKAALQGEQKSNWVVVGA
                     GYAGLAAARRLAENRPDDRVILLDAGEVGENASGRNSGFAIDLPHNVGSSMEELEGSH
                     RFMALARAAIDYHEAQIERYGIDCDWTQPGKYHCAVSQQGVREVLEPFARELDALEEP
                     YTWIEKDELDRRLGTTHFAAGVHTPGCRLMNPAALVRGLADNLPDNVTLYENSPVTAI
                     ELERGVRLSTPNGQVTAEQMILGVNGFADYFGYFEQRLLPMAANASLSRQLTLAERNA
                     LGCEENWGVTPANAFASITMRYTNDHRILIRNNIYHNRKMREPESHRRHIKQVHKRLF
                     DERFPMLPEVDMEYTWTGFLCLAQNGAPGFGKLADNVYTSVCQNAVGVTKGTAGGMLA
                     ADMACGIDNELIGYMESLGTPNKLPPRPFLDIGVKARLAWELWKARKEV"
BASE COUNT          483 a          670 c          724 g          442 t
ORIGIN      
        1 ggattcgcga cgagcgtcgc gaccattccc ggattgcgag gtcgagccgt gaatcgaagt
       61 cggccgcatc gatacaggtt tcgaagaaac ggaaaatacc ttcggccagg cttgcggcat
      121 tgtttaccga tcgcgtgagg gccgcggtgt tcttgtcttt ccagtagctg accagcgcgt
      181 cgatcaagtc ctcgcgattc tcgaaatgcc agtaaaagct gccacgggtc accccgaggt
      241 cgtcggccag gcgggtgatg cgcacggcgt cgattccttc ggatacgaaa acctcgagcg
      301 cgcattgcag ccagtcgata cggcgcagct gtttgcgatc ctggggtggg gcgccgcgct
      361 cggccgccgc cttgagtaga ctggtatcgg gcattggttg cgccatacat agttgtatgt
      421 ttgaatatac agatctgtat gttttatgca aacaaaaaat agaatgacaa acagaaaagc
      481 tgctaccagg ttgatgcgct tcgacgatct cgagttcgcg ccgcgcttcg aatacggtga
      541 aatggcacag gtggccgggg tctgtggcgc cgaggatggg acccggctgg gcgccggctg
      601 ggggcgcatg accaacgcgc atatcccgtg gacgatcaaa tacgacgaag tactcaccgt
      661 gttcgagggt gttctaaggc tgcatgccaa tggcgaggta cacgagctgc gccccaggga
      721 ttgtatctgg ttgccgcagg gcaccgaatt gatctacgag gccgagtatg cgctggtgca
      781 cttcgcgatt catccatcga actggcacga ggactgagat gtgaataccc gctccgtaac
      841 gcatctaccg agagatgaca acaccaatgg ctggagccat atactcgcgc cgcgcacgcc
      901 caaagcggcg ttgcagggcg aacagaaaag caactgggtg gtcgtcggtg ccggctatgc
      961 cggtctcgcg gccgcgcgcc gtctcgccga aaaccggccc gacgaccggg tgattctgct
     1021 cgacgcgggc gaggtgggag aaaacgcctc cgggcgcaac tcgggtttcg ccatcgattt
     1081 accgcacaac gtcggctcgt cgatggagga actggaagga tcgcatcgtt tcatggcgct
     1141 ggcgcgcgcc gcgatcgatt accacgaagc ccagatcgag cgttacggca tcgattgtga
     1201 ctggacccag cccggcaagt atcactgcgc ggtatcgcag cagggtgtgc gcgaagtgct
     1261 cgaacctttc gccagggaac tcgacgcgct cgaagagccc tatacctgga ttgaaaagga
     1321 cgagctcgac aggcggctcg gcacgacgca ttttgccgcc ggcgtgcata ccccgggttg
     1381 tcgcctgatg aacccggcgg cgctggtgcg tggcctcgcg gataacctcc ccgacaatgt
     1441 aacgctgtac gaaaattcgc cggtaaccgc gatcgagctg gagcgggggg tcaggttatc
     1501 gacgccgaac gggcaggtga ccgcggagca gatgattctc ggcgtcaacg gttttgccga
     1561 ttacttcggt tacttcgaac aaaggctgct gccgatggcg gccaacgcca gcctcagtcg
     1621 tcagctgacc ctggcagagc gcaacgcgct cggctgcgag gaaaactggg gcgtgacgcc
     1681 ggccaacgcg tttgcctcga tcacgatgcg ttataccaat gaccaccgca tcctgattcg
     1741 taacaatatt taccacaacc gcaagatgcg cgaacccgaa tcccaccgca ggcacatcaa
     1801 gcaggtgcac aagcggctgt tcgacgagcg ttttccgatg ctgcccgaag tcgacatgga
     1861 atacacctgg accggctttc tttgcctcgc gcaaaacggg gctcccggtt tcggcaagct
     1921 tgcggataat gtttacacct cggtgtgcca gaacgcggtc ggggtcacca agggtacggc
     1981 tggcggtatg ctcgcggccg atatggcctg cggcatcgac aacgaactaa tcgggtacat
     2041 ggaaagcctg ggcacgccga acaagctgcc gccacgcccc ttcctcgaca ttggcgtcaa
     2101 ggcccgcctc gcctgggaat tgtggaaagc gcgtaaagaa gtttagcggt aattgctgca
     2161 gctgccctgg tctctgcgcc acagcaccgg gaaccagtcg cggcgcattt tatcgccgtg
     2221 tttcatgccg tgatcgacgt aaggcggtga ggtttcaccc gcgcgttcgc agtaggttgc
     2281 gtcgtcgccg agccagcgag tcgaaaaagc gcgcctgat
//
LOCUS       DATCZV010000341         6411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_345438, whole genome shotgun sequence.
ACCESSION   DATCZV010000341 DATCZV010000000
VERSION     DATCZV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345438"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VIW27_07655"
     CDS             complement(<1..1113)
                     /locus_tag="VIW27_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5739586.1"
                     /translation="MSLVTPLLLLLAILRTPLFVVIALGAMLGFYGQGIDLSVISIEI
                     YRLAEMPVLIAIPLFTFAGYLLGKGNASTRLVDLTNSLLGWMPGGLAIVSIIACALFT
                     AFTGASGITIVALGGLLFTSLTRAGYSEKFNLGLLTSCGSLGLLFAPSLPLILYGVVA
                     QQMDLPQGVRISDLFLAGILPGLLMLFMLSAQAMLQPRKAPPRESFDRTAALAALRAA
                     KWEIPLPFLVLGGIYGGYFALSEAAAVTAVYVLVVEVLIIREIRVTDLPAIVRESMTL
                     VGGILIIIGVSLASTNYLIDAQVPTRMFEFVQQHIDDKLTFLILLNLFLLGLGMMLDI
                     FSAIVIIVPIILPIAVGYGIHPVHLGIIFLANMQLGY"
     gene            complement(1110..1613)
                     /locus_tag="VIW27_07660"
     CDS             complement(1110..1613)
                     /locus_tag="VIW27_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5739587.1"
                     /translation="MPAWRQRIHRLEDALLVTLLAVMIGLAATQIVMRNLFDSGFVWI
                     DPTLRVLVLWLGLLGAMVATRENRQISIDLLTRYLKGKRQHLLRAAVEQVSAWTCLVI
                     AAYGFNWIRLDFVDGLVAFAGIPAWLLEIIIPFSFAVIGLRYLLMSWASARLYLDPHG
                     SSSETAR"
     gene            complement(1617..2621)
                     /gene="dctP"
                     /locus_tag="VIW27_07665"
     CDS             complement(1617..2621)
                     /gene="dctP"
                     /locus_tag="VIW27_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY5739588.1"
                     /translation="MIRLGLKIFAIACLSLVLMAGARASVLKIATLAPAGTNWMKQMT
                     AGANAIEQRTAGRVKLKFYPGGVMGNHQSVHRKIRIGQLQGGAFTPGGLANVNPDIQV
                     LGLPMLFRDLEEVDYVRQRLDPVLKQRMADSGFVILGITEGGFARILSKQPMQNLESL
                     RNARVWVPEGDELGLTVFKALGITPISLPIGDVFTGLQTGLIDTVAVNPTSAIAFQWH
                     SSTEFMTEVPIVYLIGVLAVQKKALDRLSADDRAIVVEEMGKVFASMDVGNRADDQGA
                     IEALKSHGISFVPADSGEAERWEEIAGQAIDKMVVDGLIDADLVARVRDQLRTLRDLQ
                     "
     gene            complement(2671..3522)
                     /locus_tag="VIW27_07670"
     CDS             complement(2671..3522)
                     /locus_tag="VIW27_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEY5739589.1"
                     /translation="MSRMPILLLLSVCLLSGCASLVGSATSRMADDIALAIRNQNDPS
                     TVRDGAPAYLLMIDGLIAGDPENTDLLLAGAGLYGSYSGAFVDDEARAAALADKALAY
                     ARRALCLELGRVCEASGGRLDEFTASLAATDASNIEVLYSFATSWAGWVQANASDWNA
                     VADLAKVEALFAHCLAVDERHDGGGAHVYLGVIKTLRPAALGGEPEIAREHFERAIEI
                     SAAHNLMYKVLMARHYARNVFDRELHDRLLNEVLEAEAEYPGLTLVNTLARREAEILL
                     AEGDDFF"
     gene            3785..4585
                     /gene="cpdA"
                     /locus_tag="VIW27_07675"
     CDS             3785..4585
                     /gene="cpdA"
                     /locus_tag="VIW27_07675"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF008359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HEY5739590.1"
                     /translation="MANYFDTVTLDPADTAGVRIVQLTDCHILAGDGERLRGVDTRRS
                     FEAVLAAALRDNSRIDLILATGDLAEDGSAEAYRYLAASFTAAGLPTFWLPGNHDDPA
                     TMRTHLAGAAMRDARLVLSGNWLILMLDSTLAGQVRGRVAEAELDFMDGALRRHADRH
                     ALVCLHHQALEADSEWIDAKGLENSAQLRDRLTAHANVRGVLWGHVHQELYRHRDGIE
                     WMSTPSTCVQFMPGSREFAVDDQPPGYRYLVLGEDGAIETGVRRLPPV"
     gene            4877..5278
                     /locus_tag="VIW27_07680"
     CDS             4877..5278
                     /locus_tag="VIW27_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5739591.1"
                     /translation="MTMLLLLVLLPFTGHTAAAERWFDPPAVELGNQLFQQHCAACHG
                     SEAQGTENWKQTDADGIYPPPPLNGTAHAWHHSIPQLARSIKEGGVKLGGKMPPFGDR
                     LNNGEILALIAYFQSKWPDELYQAWHQRFLQ"
     gene            5327..5626
                     /locus_tag="VIW27_07685"
     CDS             5327..5626
                     /locus_tag="VIW27_07685"
                     /inference="COORDINATES: protein motif:HMM:NF021620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HEY5739592.1"
                     /translation="MSLGKLFKSLLGSATSSASPAVEPEDYKGFSIEAAPIDEDGKFR
                     TAGYISGELDGEARRVRFIRADQSSTMQAAIEHSLTKGRQIIDEQGTRLLQKEQL"
     gene            complement(5653..6342)
                     /locus_tag="VIW27_07690"
     CDS             complement(5653..6342)
                     /locus_tag="VIW27_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017032254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5739593.1"
                     /translation="MIELRPSEERGPANHGWLQSRHTFSFAGYYDPRHMGVSALRVIN
                     DDEVAPGAGFATHSHQDMEIISYVKRGSIEHKDSMGNVERLPAGEFQLMSAGTGVTHS
                     EYNPSDSEPLEFLQIWIIPNQLGIEPGYQQKKFPERDGLQLIASPDARDGSLRLHQDA
                     SLYQLRLEAGDSASHSLAAGRRIYVHLVGGRIEVDGELLGEGDGATLSATDRVVFHGE
                     TRAEALVFDLP"
BASE COUNT         1299 a         2038 c         1945 g         1129 t
ORIGIN      
        1 gtagccgagc tgcatgttgg cgagaaagat gatgccgagg tgcaccgggt ggatgccgta
       61 gccaaccgcg atcggcagaa tgattggtac gatgatgacg atcgccgaaa aaatgtcgag
      121 catcatgcca agccccagca ggaacaggtt gagcaggatc agaaaggtca gcttgtcgtc
      181 gatatgctgc tgcacgaact cgaacatgcg cgtcggcacc tgcgcgtcga tcaggtaatt
      241 ggtcgacgcc agcgacacgc cgatgatgat caggatgccg ccgacgagcg tcatcgactc
      301 gcgcacgatc gccggcagat cggtaacgcg aatttcgcga atgatcagca cttcgaccac
      361 cagcacgtag acggcggtca ccgcggcagc ttcggacaac gcgaagtaac caccgtagat
      421 tccgccgagc accaggaacg gcagaggaat ttcccacttc gccgcacgca acgcggcgag
      481 tgcggcggtc cggtcgaacg actcccgggg aggcgccttg cgcggctgca gcatcgcctg
      541 tgccgacagc ataaacagca tcagcagccc cggcagaata ccggccagga acaagtcgct
      601 gattctcaca ccctgtggca ggtccatctg ctgggcgacc acgccgtaca gaatcaacgg
      661 cagcgacggc gcgaacagca ggcccaggct gccgcaactg gtcaacaggc cgagattgaa
      721 tttttccgag tagccggctc gcgtcagcga cgtgaaaagc aatccgccga gggcgacgat
      781 agtgataccc gaggcgccgg tgaacgcggt aaacagtgca caggcgatga tggacacgat
      841 cgccaggccg ccgggcatcc agccgagcag actgttggtg aggtcgacca gccgcgtcga
      901 tgcattgccc ttgccgagca ggtaaccggc aaaggtgaac agcgggatcg cgatcagcac
      961 cggcatctcg gcgagccgat agatttcgat gctgatcacc gacagatcga taccctggcc
     1021 gtaaaagccg agcatcgcgc cgagcgcgat gaccacgaac agcggcgtgc gcaggatcgc
     1081 cagcaacagc agtagcggtg ttacgagact caccgggcag tctcgctgga cgaaccatgc
     1141 gggtcgaggt aaagccgtgc cgacgcccag gacatcagca aatagcgcag gccgatgacc
     1201 gcgaaactga aaggaatgat gatttcgagc agccatgccg gaatgccggc gaaggccacc
     1261 agcccatcga cgaagtccag ccgtatccag ttaaagccgt atgcggcaat caccagacaa
     1321 gtccaggcac tgacctgttc gaccgccgct cgcaacaggt gttgccgttt gcccttgagg
     1381 taacgcgtca gcaggtcgat gctgatctgc cggttttcgc gcgtggcgac catcgccccc
     1441 agcagaccca gccagagcac cagcacgcgc agggtcggat cgatccagac aaagccggaa
     1501 tcgaacaggt tacgcatcac tatctgggtc gcggcgagtc cgatcatcac cgcgagcagc
     1561 gttaccagca gggcatcctc gagacgatga atgcgctgtc gccaggcagg catcgcttac
     1621 tgaaggtcgc gcagggttcg aagttgatcg cggacccggg cgacgagatc ggcatcgatc
     1681 agtccgtcga ctaccatttt gtcgatcgcc tggccggcga tttcttccca gcgctcggcc
     1741 tcgcccgaat ctgccggcac gaaggaaatt ccatgcgatt tcagcgcctc tatcgcgccc
     1801 tggtcgtcgg cacgattgcc aacgtccatg ctcgcgaata ccttacccat ttcctcgacg
     1861 acgatggcac ggtcgtcggc cgacagccgg tccagggctt tcttttgcac cgccagtacg
     1921 ccgatcaggt agacaatcgg cacttcggtc atgaactcgg tgctgctatg ccactgaaag
     1981 gcgatcgccg aagtcgggtt gaccgccacg gtatcgatga ggccggtttg cagaccggta
     2041 aatacgtcgc cgatcggcaa cgagatcggt gtgataccga gtgccttgaa gaccgtgagc
     2101 cccagctcat cgccctcggg cacccagacc ctggcgttac gcagcgattc caggttctgc
     2161 atcggctgct tcgacaggat acgcgcaaag ccaccttcgg taataccgag aatcacgaaa
     2221 ccgctgtcgg ccattcgctg cttgagaacc ggatcgagcc gctggcgaac gtaatcgacc
     2281 tcctccaggt ccctgaacag catcggcaag ccgagcacct ggatgtcggg attgacattc
     2341 gccagtccgc ccggcgtgaa cgccccgccc tgcaactggc cgatacggat cttgcgatgt
     2401 acgctctggt ggttgcccat cacaccgccc gggtaaaatt tcagcttgac gcggccggcg
     2461 gtacgctgct cgatcgcgtt cgcaccggcg gtcatttgct tcatccagtt ggtgcccgcc
     2521 ggcgccaggg tcgcgatttt cagcacactc gcccgtgcgc cggccatcag caccagcgac
     2581 aggcaggcga tcgcaaaaat ttttaagcca agtctgatca taactttcta gtctatccgg
     2641 tatttgagac ggctgcccgt caatagtttc ctagaaaaaa tcgtcgcctt cggcaagcag
     2701 aatctcggct tcacggcgcg ccagcgtatt caccagcgtc aggcccgggt attcggcttc
     2761 cgcctccagc acttcgttca acagccgatc gtgcagctca cggtcgaaaa cattgcgcgc
     2821 gtaatgcctg gccatcaata ccttgtacat caggttgtgt gccgccgaga tctcgatcgc
     2881 acgctcgaag tgctcgcgcg cgatctcggg ctcaccgccg agggcggccg ggcgcagcgt
     2941 cttgatcacg cccaggtaaa catgcgcgcc accaccgtcg tggcgctcgt caactgccag
     3001 gcagtgcgca aacaaggcct cgaccttggc caggtcggcg accgcattcc agtccgaggc
     3061 gttggcctgt acccagcccg cccacgaggt cgcgaaactg tacagcacct cgatattcga
     3121 cgcgtcggta gcggcgaggc tggcggtgaa ttcgtcgagg cggccgccgc tcgcctcgca
     3181 aacccggccg agctccagac acagcgcccg gcgcgcgtag gcgagtgcct tgtcggcgag
     3241 cgctgccgca cgcgcctcgt cgtcgacgaa agccccgctg taggacccgt agagaccggc
     3301 accggccagc agcagatcgg tgttttcggg atcgcccgcg atcaggccat cgatcatcag
     3361 caggtaagcc ggggcgccat cacgcaccgt cgacggatcg ttctgattgc ggatcgcgag
     3421 cgcgatatcg tcggccatgc gcgacgtcgc cgagccgacc agcgaggcac agccggacag
     3481 caggcagaca gacagcagca gcagtatggg cattctgctc atcgatcagt atcctcgccc
     3541 cggcttaccg cgagtgtcgc gaggcaggca gcggccttcg tatgcaatat ctaatttcac
     3601 tcatccctcg gaatctgcag tggtaaccta gtttatatcg caccaatttt aaagggttgg
     3661 aacggcaaaa tcgcgtttgc gtcgactcca gccggcctcc accggtatac cgcgctggcg
     3721 cgattgaggg tgccggcgat acctgctatc gtctgcggcg cggcagcagc ggagtatgca
     3781 cggcatggcg aactatttcg acacggttac cctcgatccg gccgacacgg ccggcgtgcg
     3841 aatcgttcag ctgaccgact gccacattct ggccggcgac ggggagcgcc tgcggggtgt
     3901 cgatacgcgc cgcagtttcg aagcggtact ggcggcggcg ctgcgggaca acagccgtat
     3961 cgacctgatc ctcgccaccg gcgatcttgc cgaggatggc tcggcggagg cctaccgtta
     4021 cctggccgca agcttcaccg ccgccggcct gccgactttc tggctgccag gcaaccatga
     4081 cgatcccgcc acaatgcgca cacacctcgc cggtgcagcg atgcgcgacg cccgcctggt
     4141 gctgtccggc aactggctta tcctgatgct ggattcgacc ctggccggac aggtgcgggg
     4201 gcgggtcgcg gaagccgaac tggatttcat ggacggcgcg ctgcggcgcc atgccgaccg
     4261 ccatgcgctg gtttgtctgc atcaccaggc gctcgaagcc gacagcgagt ggatcgacgc
     4321 caaggggctc gaaaattcgg cgcagctgcg cgacaggctg acagctcacg ccaatgtcag
     4381 aggcgtgctc tgggggcacg tgcaccagga attgtatcgg caccgcgacg gcatcgagtg
     4441 gatgtcgacg ccgtcgacct gcgtgcaatt catgcccggc agccgtgaat tcgcagtcga
     4501 cgatcaaccg cctgggtacc gttacctggt gctcggtgag gacggcgcta tagaaaccgg
     4561 ggtgcggcga ctgccgccgg tctgagccct gccggccagc ggcacgagca tcgaccgggc
     4621 agttacccct gcccgcagcc gtcagcgcgc gccgtgcatc cgggctcaca ccgcaaacgg
     4681 gctgtatcag tccccttccc aaggtggaca tcgcctctcg ctgcggataa aaagccggga
     4741 atatgaaacc cttgagcggc tcaggggtct agagaaggac cgccaaagat tgccgggttt
     4801 tgtcagccgt cgacaccgag gttgaaccga ttccgcgatc cacggcacct gacctgcgag
     4861 ataaccgatg cgatcgatga caatgctttt gttactggtg ctgctgccat tcaccggcca
     4921 taccgccgcc gccgagcgct ggttcgaccc gccggccgtg gaactcggca atcagctgtt
     4981 ccagcagcac tgcgccgcct gccacggcag cgaggcacag ggcaccgaga actggaaaca
     5041 aaccgatgcc gacggcattt atccgccgcc gccgctcaac ggcaccgcgc atgcctggca
     5101 ccactcgatt ccgcagctgg cgcgatcgat caaggaaggc ggcgtcaagc tcggcggcaa
     5161 gatgccgccg ttcggcgatc gcctgaacaa cggcgagata ctggcgctga tcgcctattt
     5221 ccagtcgaag tggcccgacg agttgtacca ggcctggcac cagcgattcc tgcagtagca
     5281 cggctttctg ttatcatgga ttcccgcaat cgtcagggga ctcgccatgt cgctaggcaa
     5341 gctgttcaaa tcactgctcg gcagcgctac ttcctccgct tcgccggctg tcgaacccga
     5401 ggattacaag ggattttcga tcgaggcggc gccgatcgac gaggacggca agttccgcac
     5461 cgccggttat atcagcggcg aactcgacgg cgaagccagg cgcgtgcgct tcatccgcgc
     5521 cgaccagagc agcaccatgc aggcggcgat cgagcattcg ctcaccaagg gccggcaaat
     5581 catcgacgaa cagggtacga ggctactgca aaaggaacag ctttgaccgc gcgccctgca
     5641 tcgccgacgc gttcagggca gatcgaagac caaagcctcg gccctggttt caccgtgaaa
     5701 gactaccctg tcggtagccg atagcgtcgc accatcgcct tcgccgagca gctcgccgtc
     5761 aacctcgatt cgtccgccga ccaagtgcac gtagatgcgc ctgcccgcgg ccagtgaatg
     5821 gcttgcgcta tcccccgctt ccagccgcag ctggtaaagg ctggcgtcct ggtgcagccg
     5881 cagcgaaccg tcgcgggcat cgggtgaagc gatcaactgc agcccgtctc gctcgggaaa
     5941 cttcttttgc tggtaaccgg gttcgatgcc gagctgattg ggaataatcc agatttgcaa
     6001 aaattccagc ggctcgctgt ccgacgggtt gtactcgctg tgggtgacac cggtgccggc
     6061 gctcatcaac tggaactccc cggccggcag acgctcgacg ttgcccatgc tgtccttgtg
     6121 ctcgatgctg ccgcgtttta cgtagctgat gatttccatg tcctggtggg aatgggtggc
     6181 gaagccggca cccggcgcca cctcgtcgtc gttgatgaca cgcaacgcgg agacgcccat
     6241 gtggcgggga tcgtagtagc cggcgaacga aaacgtatgc cggctctgca gccagccgtg
     6301 attagcgggg ccgcgttctt cgctgggtct caattcgatc atgattcact cctgtggtga
     6361 tagtcgataa atgttcagca tatacgggcg gtttatggaa ataaatgcat t
//
LOCUS       DATCZV010000342         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_186782, whole genome shotgun sequence.
ACCESSION   DATCZV010000342 DATCZV010000000
VERSION     DATCZV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186782"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VIW27_07695"
     CDS             <1..764
                     /locus_tag="VIW27_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5739594.1"
                     /translation="IAATLKASELKLNYLTLEQDSLGGTVFKYPRGKIVMTQPVDLPL
                     AGKMHFKETSKEQLLEFWQQIEQRTGIEIRYREIVADIKSGQSGFEITTNKGKYSADR
                     VLLTIGRRGTPRKLGVPGEDDPKVVYQLIDQDQYRGQKVLIVGGGDSALEAALAIADA
                     DGTEVSLSYRSDSFSRAKAKNRERIDEYAAGGRIRLLLSSNVTRITPRSVTLDQQGTA
                     LDLDNDAIIVNAGGILPTGFLQKIGIGVETKYGTA"
     gene            751..>2037
                     /locus_tag="VIW27_07700"
     CDS             751..>2037
                     /locus_tag="VIW27_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY5739595.1"
                     /translation="MEQPERGSRIFTRLAMLLLAWLCLASPLRAAGDAALDEVERLIR
                     LRSYAAAVERLRPLAEDGVAAAQYRLAGLYRNGKGTDRNLEKAIDLYLDAAQAGHAGA
                     QYALASIIEKSADTPAARNDAYYWYRQAAAQDNRQALLKLEQLRHAPELDQRQIDDSD
                     IFTAIRQDQQALIKSMIAQGVDLDLTDRHGNSAVLAALLAEAPQLAAILIEHTDNFQR
                     PNAIGIRPLHVAAARGYREIVIELAYKDASLDQADAQGNTALMLALKNRRTEIAALLL
                     ELGARHDIVNLKGQSAVELAYAADNPQARALFAGLGIEPVASRAARPADPVATLKAGV
                     ARQGERYAGWPLLNIAIELGEEASVKQLLAGQANAGARDPDGNNALHVAARSADDVNL
                     RRVLDAGVEVNALNGKRETALYLAAEAGCLACVRLLL"
BASE COUNT          383 a          673 c          681 g          300 t
ORIGIN      
        1 gcatcgccgc caccctcaag gccagcgagc tgaaactgaa ctacctgacc ctggaacagg
       61 attcgctcgg cggcacggta ttcaagtacc cgcgcggcaa gatcgtcatg acccagccgg
      121 tggatttgcc gctggccggg aaaatgcatt tcaaggagac cagcaaggag caattgctgg
      181 agttctggca gcaaatcgag cagcgcaccg ggatcgagat ccgttaccgc gaaatcgtcg
      241 ccgacatcaa gagcggccag tcgggattcg agattaccac caacaagggc aagtacagcg
      301 ccgaccgcgt gctgctgacc atcggccggc gcggaacccc gcgcaagctg ggggtcccgg
      361 gcgaggacga ccccaaggtg gtctaccagt tgatcgacca ggaccagtac cgcgggcaaa
      421 aggtactgat cgtcggcggc ggcgacagtg cgctggaggc ggcgctggcg atcgccgatg
      481 ccgatggtac cgaggtttcg ctgtcctacc gcagcgattc gttttcgcgc gccaaggcca
      541 aaaaccgcga acgtatcgac gagtacgcgg ccggcggccg gatcaggctg ctgctgtcgt
      601 cgaatgtcac gcgcatcacc ccgcgttcgg tcacgctcga ccagcagggt acggcgctcg
      661 atctcgacaa cgacgcgatc atcgtcaatg ccggcggcat cctgccgacc ggcttcctgc
      721 aaaagatcgg catcggcgtg gagaccaagt atggaacagc ctgaacgcgg ctcgcggata
      781 ttcacccgcc tggccatgct gttgctggcc tggctgtgtc ttgcctcgcc gctccgggcc
      841 gcgggtgatg ccgcgctcga cgaggtcgag agactgatcc gcctgcgctc ctacgcggcg
      901 gccgtcgagc gcctgcggcc gctggccgag gacggggtag ccgcggcgca gtaccggctg
      961 gcgggccttt accgcaacgg caaggggacc gacaggaatc tcgaaaaggc gatcgatctg
     1021 tatctcgacg cggcgcaggc cgggcatgcg ggcgcccagt acgcgctggc ctcgatcatc
     1081 gaaaaatcgg ccgatacccc ggcggcgcgc aacgatgcct actactggta tcgccaggcg
     1141 gcggcccagg acaaccgcca ggcgctgctc aagctcgagc agttacggca tgcgccggag
     1201 ctggaccagc ggcaaatcga cgacagcgat atcttcaccg cgattcgcca ggatcagcag
     1261 gcgctgatca agtcgatgat cgcccagggg gtcgacctcg acctgaccga tcgtcatggc
     1321 aactccgcgg tactggcggc gctgctcgcg gaggccccgc agctggccgc aatcctgatc
     1381 gagcataccg acaattttca acggccgaat gcgatcggca ttcggccgct gcacgtcgcc
     1441 gcggcgcgcg gttaccgcga aatcgtcatc gagctggcgt acaaggacgc gagcctcgac
     1501 caggccgacg cgcagggcaa caccgcgctg atgctggcgc tgaaaaaccg ccgcacggag
     1561 atcgcggcgc tgttgctgga gctgggcgcg cgccacgata tcgtcaacct caaggggcaa
     1621 tccgcggtcg agctggccta cgcggcggat aatccgcagg ccagggcgct gttcgcgggg
     1681 ctcggcatcg agcccgttgc cagcagggcc gcgcgcccgg cggacccggt cgccacgcta
     1741 aaggccgggg tcgcgcgcca gggcgagcgc tacgcgggct ggccgttgct caacatcgcc
     1801 atcgagctgg gcgaggaggc gagtgtcaaa caattgcttg ccggccaggc caatgccggc
     1861 gcccgcgacc cggatggcaa caatgcgctg catgtcgccg cccggtccgc ggatgacgtc
     1921 aacctgagac gcgtgctcga cgccggcgtc gaggtaaacg cgctcaacgg caagcgcgaa
     1981 accgcgcttt acctggccgc cgaggctggc tgcctggcct gcgtgcgact gctgctc
//
LOCUS       DATCZV010000343         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_291826, whole genome shotgun sequence.
ACCESSION   DATCZV010000343 DATCZV010000000
VERSION     DATCZV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291826"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VIW27_07705"
     CDS             complement(<1..1464)
                     /locus_tag="VIW27_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEY5739596.1"
                     /translation="MGANYANLFEVIAGNVSATDDQTRISELCSELEAYLEPVQVEHV
                     YEAYLVGARAHEGQSRVSGEPYISHPVAVARILAEMRMDSNSIIAAILHDVIEDTPTL
                     KEHIAEQFGDEVAELVDGVSKLTQMDFHSKAEAQAENFRKMMLAMVKDIRVIIIKLAD
                     RLHNMRTLEAMPPEKRRRIAIETMEIYAPIANRLGIFKMRHELLDLSIKAAYPMRYRA
                     LETACKKVVGHRKEIFNTIEAAIKQRLKDTHIEAAVKYRQKNIHSIYSKMKEKKLSFK
                     EITDVFGVRILTHSVDDCYRVLGVMHNLYKPLPGKFKDYLAIPKTNGYQSLHSSLFGP
                     HGVSVEVQIRTEEMDHFAESGIAAHWIYKEGDGSATRAQSRALDWLKNLLEMQQKSGS
                     SQEFLESVKVDLFPDEIYVFTPKGDIKKLPRGATVVDFAYAVHTDVGDTCVGAKVNGR
                     IVPLRSTLANGDQVDIVRSKAQTPSTSWESFVVTGRAR"
     gene            complement(1600..1890)
                     /gene="rpoZ"
                     /locus_tag="VIW27_07710"
     CDS             complement(1600..1890)
                     /gene="rpoZ"
                     /locus_tag="VIW27_07710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY5739597.1"
                     /translation="MARITVEDCLEHVENRFDLVLLAARRARQISQGADPLVPPENDK
                     PTVIALREIAENLVTASGMDEMEARAEIEKIATDDDLIRQIAQASLEADDPE"
     gene            complement(2094..>2336)
                     /gene="gmk"
                     /locus_tag="VIW27_07715"
     CDS             complement(2094..>2336)
                     /gene="gmk"
                     /locus_tag="VIW27_07715"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685804.1"
                     /note="Essential for recycling GMP and indirectly, cGMP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY5739598.1"
                     /translation="VLEQRLRDRGTDDEDTIERRMRAADREMSHYNDAEYLVINDGFD
                     QALDDLDAIIHAQDLTLARQKLKNRQLVESIPPEID"
BASE COUNT          403 a          686 c          730 g          517 t
ORIGIN      
        1 gcgagcgcgg ccggtcacga cgaaggattc ccacgatgtc gagggtgtct gcgccttcga
       61 gcgcacgata tcgacctgat cgccgttggc gagggtgctg cgcaacggaa cgatgcggcc
      121 gttcactttt gcgcccacgc aggtgtcgcc cacatcggtg tgcaccgcgt aagcgaaatc
      181 gaccacggtc gcgccgcgcg gcagtttctt gatgtcgccc ttgggggtaa atacgtagat
      241 ctcgtcgggg aacaggtcga ccttgacgct ttcgaggaac tcctgcgagc tgcccgactt
      301 ctgctgcatc tcgagcaggt tcttgagcca gtccagcgcc cggctctggg ccctggtcgc
      361 gctgccgtct ccttccttgt aaatccagtg ggcggcgatg cccgactcgg cgaaatggtc
      421 catttcctcg gtacgtatct gcacttcgac cgacaccccg tgggggccga acaggctcga
      481 atgcagcgac tggtaaccgt tggtcttggg aatcgccagg taatccttga acttgcccgg
      541 cagcggcttg tagagattgt gcatgacgcc gagcacgcgg taacaatcgt ccaccgagtg
      601 ggtcagtata cggaccccga agacgtcggt gatctccttg aagctgagct tcttttcctt
      661 catcttgctg tagatgctgt gaatgttttt ctgccggtac ttgaccgccg cctcgatgtg
      721 ggtgtccttg aggcgctgtt tgatcgcggc ctcgatggtg ttgaaaattt ccttgcggtg
      781 gccgaccact tttttgcagg ccgtctcgag tgcgcggtag cgcatcgggt aggcggcctt
      841 gatcgacagg tcgagcagct cgtggcgcat cttgaagatg cccaaccggt tggcgatcgg
      901 cgcgtagatt tccatggttt caatggcgat gcggcggcgc ttctcgggcg gcatggcctc
      961 cagcgtgcgc atgttgtgca ggcggtcggc gagcttgatg atgatcacgc gaatatcctt
     1021 gaccatcgcc agcatcatct tgcggaaatt ctccgcctgc gcctcggcct tgctgtgaaa
     1081 atccatctgc gtgagcttgc tgacgccgtc gaccagttcg gccacctcgt cgccgaactg
     1141 ctcggcgata tgttccttca gcgtcggggt atcctcgatg acgtcgtgca ggatcgcggc
     1201 aatgatactg ttgctgtcca tgcgcatctc ggcgaggatg cgcgccaccg cgaccggatg
     1261 gctgatgtag ggctcgccgg aaacccggct ctggccctcg tgggcacggg cgccgaccag
     1321 gtaagcctcg tagacgtgct cgacctgcac cggttccagg taggcttcga gttcgctgca
     1381 gagttcggaa atgcgggtct ggtcatcggt ggcgctgacg ttgccagcga tgacttcgaa
     1441 cagattggcg tagttcgccc ccattgtttc cgctaaatga ccatgcacgc tattacaatg
     1501 ggggtttata acacatttcc aagctgctgg cgcgcatacg caaggccccg cgaagcctgt
     1561 tcgcggggct tgaaaacgta ctggaatcag ggggtttagt tactcggggt cgtcggcttc
     1621 gagactggcc tgggcgatct gccgaatcag gtcgtcgtcg gtcgcgatct tttcgatttc
     1681 ggcgcgggct tccatttcgt ccatacccga agccgtgacc aggttttcgg cgatttcgcg
     1741 cagcgcgatc accgtcggct tgtcgttctc cgggggcacc agtggatccg ctccctgcga
     1801 aatctggcgc gcacggcgag ccgccagtaa caccaggtca aagcggtttt cgacgtgctc
     1861 gagacagtct tctacggtaa ttcgcgccat tgcttgcagc cctgctcaaa aaaggggcgc
     1921 aatgatagct aaaaagccgt tgaatctaaa ggcccgcagc aaaaaaaagc ccgcctgcct
     1981 cacgggggcg aatcgctagc ggccaccgga ccggtgaaag gatggtcatt ttgcggcggc
     2041 cgagccaagc ataccgcgca ccatcacagg gggcgatggg ctcccgccgc cggctagtcg
     2101 atctccggcg gaatcgattc gaccagctgg cggttcttga gtttctgccg cgcgagcgtc
     2161 agatcctgag catggatgat cgcatcgagg tcgtccagcg cctggtcgaa gccatcgttg
     2221 atgaccaggt attcggcatc gttgtagtgg gacatttcgc gatcggcggc acgcatgcgg
     2281 cgctcgatgg tgtcctcgtc gtcggtaccg cggtcgcgca gccgctgctc gagcac
//
LOCUS       DATCZV010000344         3986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_504123, whole genome shotgun sequence.
ACCESSION   DATCZV010000344 DATCZV010000000
VERSION     DATCZV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504123"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VIW27_07720"
     CDS             complement(<1..828)
                     /locus_tag="VIW27_07720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5739599.1"
                     /translation="MKDYDVAIVGGGLLGSAFAWGLADQGLDTIVCDEGDNAIRTARG
                     NFGLVWVQGKGAGMPEYARWSLQSSLAWTGFAARLKGDTGTDLHYHRPGGFNICLDDD
                     DLADNVALLERLRRESGDHGYEFEIVEHAELKRRMPIVGDVPGATYCPHDGHCNPLLL
                     LRALHAGYLAMGGEYRPWSRIVRIESVGDGFVLHGDDGAVVCAAEKLIIAAGHGSREL
                     GAQVGLEVPIHPDQGQVIVTEKSLPVLEYPTNYVRQTDDGNFLLGPSSKDAGFDLDTE
                     "
     gene            complement(815..1819)
                     /locus_tag="VIW27_07725"
     CDS             complement(815..1819)
                     /locus_tag="VIW27_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5739600.1"
                     /translation="MAVLELDRLTRHFEIRPGLIERRLHKAPTRYIKAVEGLNLSVGE
                     NEILGIAGESGCGKSTTCTMIAGLLEPTSGDIRYRGKSLVGLEGEEKREHHRRVQMIF
                     QDPYESLNPRFRIVDIIAEGPRALDMWSDAEILDRTEAMLDGVGLNPAKYADRFPHEL
                     SGGERQRVGIASALVMEPEMVIADEPLSMLDVSIRAGILDLLKGLQRQRRFSSVYVSH
                     DLSILGNVADRLMIMYLGQAMEIGPVEEVVRRPKHPYTRALIGAVPVPDPRVRRPVPK
                     ISGDISRPIDPPPGCRFAPRCPYADASCGETFLQLDTTDPAQHQAACHRLHLLPDEGL
                     "
     gene            complement(1807..2829)
                     /locus_tag="VIW27_07730"
     CDS             complement(1807..2829)
                     /locus_tag="VIW27_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739601.1"
                     /translation="MQPQRETVLQVRGLGVRYQTRQGPVHAVTDMTFDVFANEVFGIA
                     GESGCGKSTLIRALLRLLPDNADVEAERASYRGQDLLTMREDEFRQRILWQEISLVPQ
                     SAMNSFNPVYRVGDQVVESIRAHRDSSRAEARERVAELFDIVGLQADLMRSYPHEFSG
                     GMRQRAMIAMALALDPGLIVMDEPTTGLDVLVQERILRRIREIRGEIESSIILITHDI
                     AVIAEMADRIAVMYGGELMEQASAIDLFETPYHPYTLGLNNAFPSIRDIGGELISIPG
                     SPPTLIGPVESCPFESRCPFRIERCAEANPAPVEIGEFHQVRCFRTDEPEELRRLAKE
                     KSTWRS"
     gene            complement(2841..3740)
                     /locus_tag="VIW27_07735"
     CDS             complement(2841..3740)
                     /locus_tag="VIW27_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739602.1"
                     /translation="MSASTINRYLQTLRRGLAEHYRIITSNPLSRIGFFVIIAFVVVA
                     IFAPWIAPYDPWEMVYDKDGKFASLRPPSWEFPVGTTHLGYDLFSQLVLATRTTLIIG
                     SVSGLISIVIGANVGLIAGYYGGKIDEILMRLTDILYGMPFLPFIIVLIALFGRKLEF
                     VILAIVLIIWRTSARVIRAQTISIKQRQFIAAARARGCSDLRIIYRHILPNVLPLLLL
                     YAAFNIAWSIITEASASFLGFGDGETISWGGMLYELWLSGKTRVAWWWFATPSICIVL
                     LVTSLVFVSRAYEEVANPRLRKR"
     gene            complement(3730..>3986)
                     /locus_tag="VIW27_07740"
     CDS             complement(3730..>3986)
                     /locus_tag="VIW27_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5739603.1"
                     /translation="WVLMNSMIELGITQAASLQLGCTLDNLMDCGHAYMSTLRMADDV
                     TDFSDRVVDGRARLPDAAGLGIEVSLDKIREYEVEHFHVG"
BASE COUNT          720 a         1268 c         1314 g          684 t
ORIGIN      
        1 ctcggtgtcc aggtcgaagc ccgcgtcctt gctcgacggt ccgagcagga agttgccgtc
       61 gtcggtctgg cgcacgtagt tggtcgggta ttcgagtacc ggcagggatt tttcggtgac
      121 gatgacctgg ccctgatccg ggtggatcgg cacttcgagc ccgacctggg cgccgagttc
      181 gcgcgagccg tggccggcgg cgatgatcag cttttcggcg gcgcagacga cggcgccgtc
      241 gtcgccgtgc agtacaaatc cgtcaccgac ggattcgatc cgtacgatgc gcgaccatgg
      301 ccggtactcg ccgcccatcg ccagataacc ggcgtgcagc gcacgcaaca gcagcagcgg
      361 gttgcaatgc ccgtcatgcg ggcaataggt cgcgcccggc acgtcgccga cgatcggcat
      421 gcgccgcttc agctcggcgt gctcgacgat ctcgaactcg tagccgtgat cgccggattc
      481 ccggcgcagt cgctcgagca gcgcgacgtt gtcggcaaga tcgtcgtcgt cgagacagat
      541 gttaaatccg ccggggcggt ggtaatgcag gtcggtcccg gtatcgcctt tcaggcgcgc
      601 ggcgaaaccc gtccacgcca gcgacgattg cagcgaccaa cgcgcgtact cgggcatgcc
      661 ggcgcccttg ccctgcaccc agaccaggcc gaagttgccg cgcgcagtac ggatcgcatt
      721 gtcgccctcg tcgcagacga tggtgtcgag cccctggtcg gcgagacccc aggcgaaggc
      781 ggagccgagc aggccgccgc cgacgatggc gacgtcatag tccttcatcg ggcaacaggt
      841 gcagacgatg gcaggcggcc tggtgctgcg ccggatccgt ggtatccagc tggagaaagg
      901 tttcgccgca ggaggcgtcg gcataggggc agcgcggggc gaagcgacaa cccggcggcg
      961 ggtcgatcgg ccgcgagatg tcgcccgaaa tcttcggcac cggacggcgc acgcgcggat
     1021 ccggcactgg caccgcgccg atcagggcac gcgtgtaggg gtgtttcggc cgccgcacga
     1081 cctcctcgac cgggccgatt tccatcgcct ggccgaggta catgatcatc aaccggtcgg
     1141 cgacgttgcc gagaatcgac aggtcatgcg acacgtaaac gctggaaaac cggcgctggc
     1201 gctgcaatcc cttgagcagg tcgaggatgc cggcacgaat cgacacgtcg agcatcgaca
     1261 gcggttcgtc ggcgatcacc atttccggct ccatcaccag cgcgctggcg attccaaccc
     1321 gctggcgctc gccgccgctg agttcgtgcg ggaagcgatc ggcgtacttg gcgggattga
     1381 ggccgacgcc gtcgagcatg gcctcggtgc ggtcgaggat ttcggcgtcg ctccacatgt
     1441 cgagcgcacg cggaccctcg gcgatgatat cgacgatgcg gaaccgcggg ttaagcgatt
     1501 cgtaggggtc ctggaagatc atttgcacgc ggcggtgatg ctcgcgcttc tcctcgccct
     1561 cgagaccgac cagcgacttg ccgcgatagc gaatgtcgcc cgaagtcggt tccagcaggc
     1621 cggcgatcat cgtacaggtg gtcgacttgc cacagcccga ttcgccggcg atgccgagga
     1681 tctcgttttc accgacgctc aggttgagcc cttcgaccgc cttgatgtag cgcgtcggcg
     1741 ccttgtgcag gcggcgctcg atcagccccg gacggatctc gaaatgacgc gtcagccggt
     1801 cgagttctag gaccgccatg tcgatttctc cttcgccagg cggcgcagct cttcgggctc
     1861 gtcggtgcga aagcacctga cctggtggaa ttcaccgatc tcgaccggcg ccgggttggc
     1921 ctcagcgcag cgctcgatgc ggaacgggca gcgcgattcg aacgggcagg attcgaccgg
     1981 gccgatcagc gtcggcggcg aaccgggtat cgagatcagc tcgccgccga tatcgcgaat
     2041 cgacggaaaa gcgttattga ggccgagcgt gtaagggtga tagggcgtct cgaacaggtc
     2101 gatggcactg gcctgttcca tcagttcgcc gccatacatg accgcgatgc gatcggccat
     2161 ctcggcgatc acggcgatgt cgtgggtgat gaggatgatc gagctttcga tttcaccgcg
     2221 aatttcgcgg atgcgccgca ggatgcgctc ctgtaccagt acgtcgagcc cggtggtcgg
     2281 ctcgtccatc acgatcagtc cgggatcgag cgccagcgcc atcgcaatca tcgcccgctg
     2341 gcgcatgccg ccggaaaact cgtgcgggta gctgcgcatc aggtcggcct gcagaccgac
     2401 gatatcgaac agctcggcaa cccgttcgcg cgcctcggca cggctgctgt cgcggtgcgc
     2461 ccgaatcgac tcgaccacct ggtcgccgac gcggtaaacc gggttgaaag agttcatcgc
     2521 gctttgcggt accagcgata tttcctgcca caaaatgcgc tggcggaatt cgtcctcgcg
     2581 catcgtcagc agatcctggc cgcggtaact cgcgcgctcg gcttcgacgt ccgcgttgtc
     2641 cggcagcagc cgcagcagtg cgcggatcag ggtcgacttg ccgcagcccg attcgccggc
     2701 aatgccgaac acttcgttgg cgaaaacgtc gaaagtcatg tcggtcaccg cgtgcaccgg
     2761 cccctgacgg gtctggtagc gcacgccgag gccgcgtacc tgcagcacgg tttcgcgttg
     2821 cggttgcatg gccggctcgt tcaccgcttg cgcagcctcg ggttggccac ttcctcgtag
     2881 gcgcggctga cgaacaccag cgacgtgacc agcagcacga tgcagatcga cggtgtcgcg
     2941 aaccaccacc aggcgacccg cgtcttgccc gacagccaca gctcgtagag catgccgccc
     3001 cagctgatgg tctcgccgtc accgaagccg aggaaactcg cgctggcctc ggtgatgatc
     3061 gaccaggcga tgttgaacgc cgcgtacagc agcagcaacg gcaacacgtt gggcaggata
     3121 tggcgataaa tgatgcgcag gtcgctgcag ccgcgcgccc gggctgcggc gatgaactgg
     3181 cgctgcttga tggagatcgt ctgcgcgcga atcacccgcg ccgaggtgcg ccagatgatc
     3241 agcacgatcg ccaggatgac gaattcgagc ttgcgtccga acagcgcgat cagcacgatg
     3301 atgaagggca ggaacggcat gccgtagagg atgtcggtca ggcgcatcag gatttcgtcg
     3361 atcttgccgc catagtagcc ggcgatcagg cctacattgg cgccgatcac gatcgagatc
     3421 agccccgata ccgaaccgat gatcagcgtc gtgcgcgtgg ccagcaccag ctgcgagaac
     3481 aggtcgtagc cgaggtgggt cgtaccgacg ggaaattccc acgacggcgg ccgcagcgaa
     3541 gcgaacttgc catccttgtc gtagaccatt tcccacggat cgtagggcgc gatccacggc
     3601 gcgaagatgg cgaccaccac gaacgcgatg atgacgaaga aaccgatgcg gctgagcggg
     3661 ttcgaggtga tgatccggta gtgctcggca aggccccgcc tgagcgtctg caggtaacga
     3721 ttgatggtgc tagccgacat ggaaatgctc cacttcgtac tcgcggatct tgtccagcga
     3781 gacctcgatg ccgaggcccg ctgcgtccgg cagccgcgcc ctgccatcga cgacccggtc
     3841 ggaaaaatcg gtgacgtcgt cggccatgcg cagcgtcgac atgtaggcgt ggccgcaatc
     3901 catcaggtta tccagcgtgc agccgagttg cagcgacgcc gcctgggtaa tgccgagctc
     3961 gatcatgctg ttcatcagca cccact
//
LOCUS       DATCZV010000345         5473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_716535, whole genome shotgun sequence.
ACCESSION   DATCZV010000345 DATCZV010000000
VERSION     DATCZV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716535"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VIW27_07745"
     CDS             <1..737
                     /locus_tag="VIW27_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739604.1"
                     /translation="WRPACLRAAFLVVCMFCFFAGAPVLTVAQMAAGLYTYPLFVSLL
                     AGPVLGEQVGPWRIGALLLGAVGAAFVLSPWQEGFSAWQLMPVIAGFLFACNVLTLRR
                     GCRHESPLALATVVGIAYVLSGTAGILLLELLPLSPSTRAALPFIAVGWPQLTLMVGA
                     FAVFASLLNLTGNICISRAYQTADASWLAPMDFSYLIFAALWSRAIFDQWPSRESVIG
                     MLLIGAAGIITAWREQVKASGRRMLQ"
     gene            complement(749..1210)
                     /locus_tag="VIW27_07750"
     CDS             complement(749..1210)
                     /locus_tag="VIW27_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5739605.1"
                     /translation="MRKFFAWLTPLVGLVFALCCATAVAGHRDPTPAECPQPRFTGKA
                     PDELYSRVNPLAASRGNRNTGSKLYHQLSDPGCSVCHGDKGEGNGQLAEQFDPRPRNF
                     ACAATIDGIPDGQLHWIIKNGSPGTAMPPFGYLSDEEIWQLVIYLRYLSGH"
     gene            1342..2259
                     /locus_tag="VIW27_07755"
     CDS             1342..2259
                     /locus_tag="VIW27_07755"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5739606.1"
                     /translation="MKQSTFPSRPMIPVPRSLVLLITVAGIALGGCSTHLGAERATTA
                     ESRDYRIDRVIESIVYTGPDWQQPLAGDLYLPERPGLLPVVLMVHGGGWANRSREDMA
                     DLAHKLTQQGYAVFNVAYRFAPRHTYPAQLEDIQQAMLWLVANAERYRLDSQRINTWG
                     YSSGAHLAALVAGVDAAEVGGIEVPPVNAVVAGGIPSDLRKYRKSPIVMRFMGGYRDD
                     MPERYAEASPAWHVSADDPPVFLYHGGLDTLVRPDQATDYYEALRSAGIDAELYLQGW
                     RGHITMFLFGGSAEARAIAFLNRHNPPSG"
     gene            2343..3497
                     /locus_tag="VIW27_07760"
     CDS             2343..3497
                     /locus_tag="VIW27_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5739607.1"
                     /translation="MADPVFPEAELQQRLSAVREAMKRHDLDALVISVPENIYYLTGL
                     DHWGFFACHVLLVPATGDMALACRAMERITVENQVTNAEFYGHADTAELSDEVLRALA
                     DRGLATARLGIEKRSLFMTPRIYERILAGAAGADWSDCSGLIDELRLVKSPLEQEYTR
                     RAAAAADAGTLAAIDALGDGASDYEVAAACHHAMIRAGSEYPGFGPFIRPTSRLGEEH
                     TTWRGDVFHNGDAVFLEIGGAFRKYQAPMGRLVYVGDAAQGAEQSAELAARGMQAICD
                     ALVPGATAGEVYQAWKDVANEAGLTDYHRHHCGYLVGIGFPPSWTGGSMVTSLQPGSG
                     RRLQSGMVFHAHSWFTNTGVADYFISNTVLLSADGAENLTPRTPATLMLR"
     gene            3711..5315
                     /locus_tag="VIW27_07765"
     CDS             3711..5315
                     /locus_tag="VIW27_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739608.1"
                     /translation="MSDKKHIKDLLTRDAREGKLGRRDFMRFAVAAGISVPLASSLWT
                     SEVAAATPKRGGTFRVGVHDMNTSDTLDPGKYQSVGDIQLAHTYRSYLTEITSDNGLG
                     PDMAISWSATPDAKTWTFELDPNATFHDGRKFTAKDAIASLNHHIGENTTSAAKALLT
                     TVTGIKAKGDNAIVIELNQGFADLPWIMTDYHLVMLPAKADGTAEWDNPIGAGPYYIE
                     KYEPGVAVSLKRHEGWHREGAYFDRIEMLQLNDPNARTTALITGEVDSITSVDLKTMN
                     LLKRNPNVAIDNTPSGSAITLPMFCDQAPFNNVDVRLALKHAIDREDIVEKIMFGTAA
                     PGNDFHVSPGMPYFPEEIPQRTYDPDKAAFHMKKSGVGSIDVRLSAADSVLPGAVDMV
                     TLYSEHAKKAGINIKPVREPNDGYWSDVWLKKPFVFVKWGARATPDQMFTLAYKDDAA
                     WNEAHWQNERFNELLLMAKAELDENRRAEQYKEMCEIARDDGGTIIPIFVNFVCARGK
                     TVMHGPSVAASWENDGARAAHRWWFA"
BASE COUNT         1025 a         1781 c         1713 g          954 t
ORIGIN      
        1 actggcgccc ggcttgcctg cgcgcggctt tcctggtggt ctgcatgttc tgctttttcg
       61 ccggtgcgcc ggtgctgacg gtggcgcaga tggccgcggg tctgtatacc tacccgctat
      121 ttgtgtcact gctggcgggc ccggtactcg gcgaacaggt gggcccgtgg cgcatcggcg
      181 cgctgctgct gggggcggtc ggcgcggctt tcgtgctcag cccgtggcag gaaggttttt
      241 ccgcctggca gctgatgccg gtgatcgccg gcttcctgtt cgcctgtaac gtgctgaccc
      301 tgcgccgcgg ttgccgccac gaaagtccgc tggcgctggc caccgtagtc ggcatcgcct
      361 acgtgctatc cgggaccgcc ggcatcctgt tgctggagct gctgccactg tcgccgtcga
      421 cgcgcgcggc gctgccgttc atcgccgtcg gctggccgca gctgaccctg atggtgggcg
      481 cgttcgcggt gttcgcgtcg ctgctcaacc tgaccggcaa catctgtatc agccgtgcct
      541 accagaccgc cgacgccagc tggctggcgc cgatggattt cagttacctg attttcgccg
      601 cgctgtggag ccgcgcgatc ttcgaccagt ggccgagccg cgaatcggtg atcggcatgc
      661 tattgatcgg cgccgccggg ataattactg cctggcgaga acaggtgaag gcctccggca
      721 ggcgcatgct gcaataatcc ccggcgaatc aatggccgct caggtaacgc aggtagatga
      781 ccagctgcca gatttcctcg tcgctgagat aaccgaacgg cggcatcgcg gtgcccggtg
      841 agccattctt gataatccag tgcaactggc cgtcgggaat gccgtcgatg gtggcggcgc
      901 aggcaaaatt gcgcggccgc ggatcgaact gctcggcgag ctgcccgttg ccctcgccct
      961 tgtcgccgtg acagacgctg caaccgggat cagagagctg gtgataaagc ttgctgccgg
     1021 tgttgcgatt gccgcggctg gccgcgagcg gattgacccg cgagtaaagc tcgtccggtg
     1081 ccttgccggt gaaacgcggc tgcgggcatt ccgccggcgt cggatcgcga tgaccggcaa
     1141 ccgccgtcgc gcagcagaga gcaaacacca ggccgaccag cggcgtcagc caggcaaaaa
     1201 actttcgcat cgcgagatta accatcgaac atttccgtgc cagactattc gcaggctaac
     1261 ttcatggcac cgtaatcaat tgacgcaggt cacatttgac ggcatcgagt aatccactct
     1321 gccggcgcga gcgtaagtga aatgaagcaa tcaacttttc cgtcgaggcc gatgattccc
     1381 gtaccacgct cgctcgtttt gctgatcacc gtcgccggca tcgccctcgg cggctgctcg
     1441 acccatctgg gtgcggagcg cgccacgacc gccgagtcgc gcgattatcg catcgaccgc
     1501 gtgatcgaga gcatcgtcta caccgggccg gactggcagc agcctctggc cggcgatctc
     1561 tatctgcccg agcggcccgg cctgctgccc gtggtgctga tggtgcatgg cggcggctgg
     1621 gccaatcgca gccgcgagga catggcggat ctggcccaca agctcacgca gcagggttat
     1681 gccgtgttca acgttgccta ccgcttcgcg cctcgccaca cctatcccgc gcagctcgag
     1741 gatatacagc aggccatgct gtggctggta gcaaatgccg agcgttaccg actcgacagc
     1801 cagcgcatca atacctgggg ttactcctcc ggcgcgcatc tcgcggcact ggttgccggc
     1861 gtggatgccg ccgaggtcgg tggcatcgag gtgccgccgg tcaacgcggt ggtcgccggc
     1921 ggcattccgt ccgatctgcg caagtaccgc aaaagcccga tcgtgatgcg cttcatgggc
     1981 ggctaccgcg acgacatgcc cgagcgttac gccgaggctt ccccggcctg gcacgtcagc
     2041 gccgatgatc caccggtttt cctctaccac ggcggcctcg acaccctggt caggcccgac
     2101 caggcgaccg attactacga ggcgctgcgc agtgccggca tcgacgccga actctacctg
     2161 cagggctggc gcggccacat caccatgttc ctgttcggcg gttccgccga ggcccgggcg
     2221 atcgctttcc tgaaccgcca caacccgccg tcaggctagt ccggcccgcc ggcgggccgc
     2281 cgatttctgt tcagcgccgc aatccattac catggccgca tcgaaatcag cgaggcgagc
     2341 cgatggccga tcccgttttt cccgaggccg aattacagca gcgcctgtct gcagtccgcg
     2401 aagccatgaa acggcacgac ctcgacgcgc tggtgatcag cgtgccggag aatatttatt
     2461 acctgaccgg actcgatcac tggggcttct tcgcctgcca tgtgctgctg gtgccggcca
     2521 ccggcgacat ggcgctcgcc tgccgcgcga tggaacgcat cacggtcgaa aaccaggtca
     2581 cgaacgccga gttttacggc cacgcggata ccgccgaact atccgacgag gtattgcggg
     2641 cactcgccga ccgcggcctg gcgacggcgc gcctcggtat cgaaaagcgc agcctgttca
     2701 tgacgccgcg catctacgag cgcattctcg ccggcgccgc cggcgccgac tggtcggact
     2761 gctcgggact gatcgacgaa ctgcggctgg tcaagtcgcc gctggaacag gaatatactc
     2821 gccgcgccgc ggccgccgcc gatgccggca cgctggccgc gatcgatgcg ctcggcgacg
     2881 gcgccagtga ctacgaggtc gccgctgcct gccaccacgc gatgattcgc gccggcagcg
     2941 agtatcccgg attcggaccg tttattcgcc cgacctcgcg cctcggggaa gaacacacga
     3001 cctggcgcgg cgacgtgttt cacaacggcg acgcggtttt tctcgaaatc ggcggtgcat
     3061 tccgcaagta tcaggcgccg atggggcggc tggtctacgt cggcgacgcg gcgcaaggcg
     3121 cagagcagtc cgccgaactg gcagcgcgcg gcatgcaggc aatttgcgac gcgctggtac
     3181 cgggggcaac cgcgggcgag gtttaccagg cctggaaaga tgtcgctaac gaggccggcc
     3241 tcaccgatta tcatcgccac cattgcggtt acctggtcgg tatcggcttt ccgccaagct
     3301 ggaccggcgg cagcatggtg accagcctgc agcccggtag cgggcgccga ctgcagagcg
     3361 gcatggtttt tcacgcccac tcctggttca ccaataccgg ggtcgccgat tatttcatct
     3421 ccaacaccgt gctgttgagc gccgacggtg ccgaaaacct gaccccgcga accccggcga
     3481 cgctgatgtt gcgctgagcg agaccgaggt cgaatcttta ccggcgttcc tgtaccatga
     3541 agcgtaaccc ggacaggtag actaccgcct cgacttgaca aaaaaagatg gcggcaatga
     3601 ttaaatgtcg tttttcccgc taatcggcga ccggtatatt cgaagcgccc gtcagccgca
     3661 atccctgccc ggacccaaaa aatcgcaaaa agccgaggag aacccaatcg atgagcgaca
     3721 agaaacacat caaagactta ctgacacgcg acgcccgcga aggcaagctg ggacggcgcg
     3781 acttcatgcg ttttgccgtg gctgccggca tcagcgtgcc gctggcgtcg agcctgtgga
     3841 ccagcgaggt cgcggcggca actccgaagc gtggcggcac tttccgcgtc ggtgtgcacg
     3901 acatgaacac ctccgatacg ctcgatccgg gcaagtacca gtcggtcgga gatatccagc
     3961 tggcgcatac ctaccgcagc tacctgacgg aaatcacctc ggacaacggc ctcggccccg
     4021 acatggcgat atcctggagc gcgacgcccg acgccaagac ctggaccttc gaactcgatc
     4081 ccaacgcaac cttccacgac ggtcgcaagt tcaccgccaa ggatgcgatc gcgtcgctga
     4141 accaccatat cggtgaaaac acgacttccg cggccaaggc gctgctgacc acggtcaccg
     4201 gcatcaaggc gaagggcgac aacgcgatcg tcatcgagct gaaccagggc ttcgcggacc
     4261 tgccgtggat catgaccgac taccacctgg tcatgttgcc ggccaaggcc gacggcaccg
     4321 ccgaatggga taacccgatc ggtgccggac cttactacat cgagaaatac gagccgggcg
     4381 tcgccgtgtc gctgaagcgg cacgagggct ggcaccgcga gggcgcttat ttcgaccgca
     4441 tcgaaatgct gcagctcaac gacccgaacg cgcgcaccac ggcgctgatc accggcgagg
     4501 tcgactcgat cacctcggtc gacctgaaga cgatgaacct gctgaagcgc aacccgaacg
     4561 tcgctatcga caacacgccg agcggctcgg cgatcacgct gccgatgttc tgtgaccagg
     4621 caccgttcaa caacgtcgac gtgcgcctgg cgctgaaaca cgccatcgac cgcgaggata
     4681 ttgtcgagaa aatcatgttc ggcaccgcgg cgccggggaa tgatttccac gtgtcgcccg
     4741 gtatgcccta cttcccggaa gaaattccgc agcgcaccta cgacccggac aaggctgcgt
     4801 ttcacatgaa gaaatccggc gtgggttcga tcgacgtaag gctgtcggcg gccgacagcg
     4861 tgctgcccgg cgcagtcgac atggtgacgc tgtacagcga acatgccaag aaggccggca
     4921 tcaacatcaa gccggtgcgc gagcccaacg acggctactg gtctgatgtc tggctgaaga
     4981 agcccttcgt gttcgtcaag tggggcgcgc gcgcgacgcc cgaccagatg ttcacgctgg
     5041 cctacaagga cgacgccgcc tggaacgagg cgcactggca gaacgagcgc ttcaacgaac
     5101 tgctgttgat ggcaaaggcc gaactcgacg aaaaccggcg cgccgagcag tacaaggaga
     5161 tgtgcgagat cgcgcgcgac gacggcggca cgatcattcc gatcttcgtc aacttcgtct
     5221 gcgcgcgcgg caagactgtc atgcacgggc cgagcgtcgc ggcgtcctgg gagaacgacg
     5281 gcgcccgcgc cgcccatcgc tggtggttcg cctgattggc gtcgttcggg cagacagtat
     5341 tataatgccc gagtcagtac tgcccgccgg gtccgtgaaa acgggcccgg cggacagatt
     5401 caataaataa ggagaagccg aatctatcaa tcgacgaggc tgttgggggg gagcctgcaa
     5461 tgggcgatat att
//
LOCUS       DATCZV010000346         2649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_559020, whole genome shotgun sequence.
ACCESSION   DATCZV010000346 DATCZV010000000
VERSION     DATCZV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559020"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VIW27_07770"
     CDS             <1..1225
                     /locus_tag="VIW27_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5739609.1"
                     /translation="AAADAEPADCVPVKGSDPLYILYTSGTTGKPKGVVRENGGHAVA
                     MKYSMSAIYDIDPGDVYWAASDVGWVVGHSYIVYGPLIHGCTTVLYEGKPIMTPDAGA
                     FWRVISEHGVKALFTAPTAFRAIKKEDPEAKLARQYDLSCLQTIFAAGERLDPPTYDW
                     LNEQFKVPVLDHWWQTETGWAIAANMHGLEPMPVKPGSSTMPVPGFNVEILDEAGKQL
                     PAGEQGFIAIKLPLPPSCLTTIWGDVERFKENYLETYPGYFTTGDGGYFDEDGYLFIM
                     GRVDDVINVAGHRLSTGEMEEVVGRHPMVAECAVIGRRDELKGQMPMGFVVLKNAEGA
                     TPEQVTQELVSMVRSDIGAVAAFKEALIVGRLPKTRSGKILRKTLRAMVDGDEYTVPS
                     TIEDPAVLDEIKALL"
     gene            complement(1504..2003)
                     /locus_tag="VIW27_07775"
                     /pseudo
     CDS             complement(1504..2003)
                     /locus_tag="VIW27_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042373.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            complement(2012..2356)
                     /locus_tag="VIW27_07780"
     CDS             complement(2012..2356)
                     /locus_tag="VIW27_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739610.1"
                     /translation="MTGSDKNLWQSLGFLRLALLALALLNALLPLVAILTDIGAGEHG
                     LWNLLVTMITPVMAPLFVVVLLFDYIMSRVRAADAEGERRLSYTRIARIELAVILLTL
                     VFWVSFFIELMS"
     gene            complement(2465..>2649)
                     /locus_tag="VIW27_07785"
     CDS             complement(2465..>2649)
                     /locus_tag="VIW27_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5739611.1"
                     /translation="HFCLRLAAVSEAEIGDHLVAHGIEAGDFEERYGAEGMGKSIYIE
                     DPEGNTVELRAQLAGQ"
BASE COUNT          506 a          872 c          825 g          446 t
ORIGIN      
        1 agccgcggcc gacgccgagc ccgccgactg tgtgccggtg aagggcagcg acccgttgta
       61 tatcctttat acctcgggca ctaccggcaa gccgaaaggc gtggtacgcg aaaacggtgg
      121 ccacgcggtg gcgatgaaat acagcatgag tgcgatttac gacatcgacc ctggtgacgt
      181 ctactgggcg gcgtccgacg tcggctgggt ggtcggccac tcctacatcg tctatggccc
      241 attgatccac ggctgcacaa ccgtgctcta cgagggcaag ccgatcatga ctcccgatgc
      301 cggggctttc tggcgggtga tctccgagca cggcgtcaag gcgctgttca ccgcgccgac
      361 cgcatttcgc gcgatcaaga aggaagaccc ggaagcgaag ctcgccaggc agtacgacct
      421 gtcgtgcctg cagaccatct tcgcggccgg cgaacggctc gatccgccga cttacgactg
      481 gctgaacgag caattcaagg ttccggtgct tgatcactgg tggcagaccg agaccggctg
      541 ggcgatcgcc gccaacatgc atggactcga gccgatgccg gtcaagccgg gctcgtcgac
      601 catgccggtg cccggcttca acgtcgagat cctcgacgag gcgggcaagc agttgcccgc
      661 cggcgaacag ggtttcatcg ccatcaagtt gccgctgccg ccgagctgcc tgaccaccat
      721 ctggggcgac gtcgagcgct tcaaggaaaa ctacctggag acctaccccg gctatttcac
      781 caccggcgac ggcggttact tcgacgagga cggctacctg ttcatcatgg gccgcgtcga
      841 cgacgtcatc aacgtcgccg ggcatcgctt gtcgaccggg gaaatggaag aagtcgtcgg
      901 ccgccacccg atggttgccg agtgcgcggt catcggccgc cgcgatgaac tcaaggggca
      961 gatgccgatg ggtttcgtgg tgctgaagaa tgccgagggt gcgaccccgg agcaggtaac
     1021 ccaggaactg gtatcgatgg tacgtagcga catcggcgcg gtcgccgctt tcaaggaagc
     1081 gctgatcgtc ggccggctgc cgaagacgcg ttccggcaag atcctgcgca aaaccctgcg
     1141 cgcgatggtc gacggcgacg agtatacggt gccatcgacg atcgaggacc cggcggtact
     1201 cgacgagata aaagccctgc tctagcggga cacggacgtc acctgacccg gcaccgtcct
     1261 gccggcctgc ggcgcaatga cggccggccg gcgtgcgtcg ccatacccgc ggaatgccgg
     1321 tcccggcgag cctaccgtca tgccggcggc atggtcaccc tgtgacccca tcgtcatgcc
     1381 ggcgaaagcc ggcatccatt acaatacgcc acctcggcat cacgccgagc tcgcgggctc
     1441 ggcggcgcct cattcaaccg gccgcagaaa tggattgcgg cctgcgccgc aatgacggca
     1501 ggactagcga ctgacggtca gttcggcagg attcgccagg gtctgcaaaa cgacgcagta
     1561 gcgctcagtg agtttcagca gggtttcgat ctgttcgtcg tccgcgtcgg tatcgaggtc
     1621 gaactgcagg tggatcgttt tgaacccgac cggcacctcg cgctcgacgc cgagcgtgcc
     1681 acgaaaatcg agatcgccct cggcgccgat cgtggtatcc cgcagttcga ttccgaacgc
     1741 ggtggccacc gcgttcaacg tcaccccggc gcaggcgacc aatgcctcca gcagcatgtc
     1801 gccagagcac agcgagctgc catcgccgcc ggtggccggg tgcaggccgg ccttggcgat
     1861 cgcgcgcccg gtatcgacct tgcaggtgac gcctgcgccg atactgccgc gcgcccttag
     1921 cgcgtgatca gcgcggctcg cgggtcatcc cggtattgct gcttgagcgg cgcctgcagg
     1981 gctttcaact gttctgctcg catggtttgc ctcagctcat cagctcgatg aaaaacgaca
     2041 cccagaaaac cagcgtgagc aatatcaccg ccagctcgat acgcgcgatc ctggtatacg
     2101 acaaacgccg ctcgccctcg gcatcggccg cgcgcacccg cgacatgatg taatcgaaca
     2161 gcagcacgac gacgaacagc ggtgccatca ccggggtgat catcgtcacc agcagattcc
     2221 acaggccgtg ttcgccggcg ccaatgtccg tcaggatggc gaccagcggc aacagtgcgt
     2281 tgagcaacgc cagcgccagc agcgccaggc gcaaaaagcc cagcgattgc cacagatttt
     2341 tatcagagcc cgtcatcagc ggaatagtgc cagttcaccc tctttgagtt aagccgtgcc
     2401 tggcgtcgat ggccgcaccg cgatcgcagc acgcgctgcg ccaggatttc gcgccgcgcc
     2461 ggggctattg gccggcaagc tgcgcacgaa gttcgacggt attgccctcg ggatcctcga
     2521 tatagatcga cttgcccatg ccctcggcgc cgtagcgctc ctcgaaatcg cccgcctcga
     2581 ttccatgcgc caccaggtga tcgccgattt ccgcctcgga aacggctgcc agccgcaggc
     2641 agaaatgat
//
LOCUS       DATCZV010000347         4252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_504678, whole genome shotgun sequence.
ACCESSION   DATCZV010000347 DATCZV010000000
VERSION     DATCZV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504678"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VIW27_07790"
     CDS             complement(<1..571)
                     /locus_tag="VIW27_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5739612.1"
                     /translation="MAETKTILLPDVGDFSDIEIIEILVASGDQVDEEQSIIVLESDK
                     ATMEIPSPFSGTIESLKVKVGDRVNEGDEIATISADGGGASTASANDSPAAADESAEA
                     EATDAANEPEAAAEQTAAESPPAAETAPKSEVAPGKIQRPHPGAASGEAPPSGQVIHA
                     SPSIRRYARELGANLEQVIGSGPKGRILKE"
     gene            complement(593..3250)
                     /gene="aceE"
                     /locus_tag="VIW27_07795"
     CDS             complement(593..3250)
                     /gene="aceE"
                     /locus_tag="VIW27_07795"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY5739613.1"
                     /translation="MVDETDIDPQETQEWIESLDSVLEREGPERAHYLLEKLIDKTRR
                     SGAYLPFSATTAYVNTIPLSQQRPIPGDQAIEHSIRSVIRWNAAAMVVHANRKSSELG
                     GHIASFASAATLYDVGFMHFFRAPSDENNGDLVFFQGHSAPGIYARAFLEGRISEEQL
                     KSFRQEALNENGLSSYPHPWLMPNFWQFPTVSMGLGPIMAIYQARFMKYLENRGIQQQ
                     DDRKVWAFMGDGEMDEPESLGAISLAAREDLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEAVFRGAGWNVLKVVWGSYWDQLFAKDNNGTLQRLMEETVDGEYQACKAKGGAYTRE
                     YFFGKYPETHDMVADMSDEDIWRLNRGGHDPHKVYAAYAEAVESKGRPTVILAKTVKG
                     YGMGEAGEGQNITHSQKKMGEEALKAFRDRFNIPISDEDIADAPFYKFADDTEEMKYM
                     RERREQLGGFMPVRRDNAEALIVPGLEIFDSLLKDSGDREFSTTMAFVRMLSTLARDK
                     NIGKNIVPIVPDEARTFGMEGMFRQLGIYAFEGQLYKPMDADQVMYYREDVAGQILEE
                     GINEAGAMSSWIAAGSAYSTHGVNMIPFYIFYSMFGFQRIGDLAWAAGDMQVRGFLMG
                     GTAGRTTLAGEGLQHQDGHSLIVASTIPNCISYDPTFAYELAVIIHSGLKRMYQMREK
                     VFYYITVMNENYSHPSMPKGCEDGIIKGLYCLDEGDEKGPKLQLMGSGTILREVIAAA
                     DLLKRDFKVNADIWSATSVNELAREARDCARWNQLHPTKKTRQSYLATQLRGRQGPVV
                     FASDYIRAYGEQLREYIWSRYVTLGTDGYGRSDTRDNLRTFFEVNRYYIVVAALKALA
                     DDGEVDSKVVQQAIKKYQIDPEKPNPVSV"
     gene            3378..>4252
                     /gene="pyrC"
                     /locus_tag="VIW27_07800"
     CDS             3378..>4252
                     /gene="pyrC"
                     /locus_tag="VIW27_07800"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5739614.1"
                     /translation="MKRLDIVKPDDWHLHLRDTPYLEAVVGDTARQFGRAIVMPNLSP
                     PVRSCADAMAYRERILAALPPHSEFEPLMTLYLTDNTSAEEIAAAAESPFIHGAKLYP
                     AGATTNSAAGVTDLDSIWPVFEAMQRHRLILQVHGEATGEQIDVFDRERVFIEQSLER
                     IRRDFPELRIVFEHITTRDAAAWVRDAGPGLAATITPHHLLINRNALFAGGLRPHHYC
                     LPVAKREHHRQALLAAATGGEACFFAGTDSAPHARDAKESACGCAGIYSAHSALELYA
                     EAFDEAGDFSHFEAF"
BASE COUNT          735 a         1348 c         1278 g          891 t
ORIGIN      
        1 cttctttcag gatcctgcct ttcgggccgc tgccgattac ctgctcgagg ttggcgccga
       61 gttcgcgcgc gtagcgccga atcgacgggc tggcgtgtat aacctggcca ctcggcggag
      121 cctcccccga agcggcgccg ggatgcgggc gctggatctt gcccggggcg acctcggatt
      181 tcggcgcggt ctcggcggcc ggcggcgact cggcggccgt ctgctcggca gcggcttccg
      241 gctcgttggc cgcgtcggtc gcctcggcct cggcactctc gtcggcggcg gccggggagt
      301 cgttcgcgga agctgtcgat gcgccgccgc cgtcggcgct gatggtggcg atttcgtcac
      361 cttcgttgac gcggtcgccg accttgacct tcagcgactc gatggtcccc gagaaggggc
      421 tcggaatctc cattgtcgcc ttgtccgact cgagcacgat gatcgactgc tcctcgtcga
      481 cctggtcgcc gctggcaacc aggatctcga tgatttcaat atccgagaaa tcgccgacat
      541 cgggaagcag gatagttttg gtttctgcca cgggctcgct cctgggcgcc ggctagacgc
      601 tgaccggatt gggtttttcg ggatcgatct ggtacttctt gatcgcctgc tgcaccacct
      661 tgctgtcgac ttcaccatcg tcggccagcg ccttgagcgc ggcgacaacg atgtaatatc
      721 gattgacctc gaaaaaggtt cgcaggttgt cgcgggtatc gctgcgccca tagccatcgg
      781 tgccgagcgt gacataacgc gaccagatgt actcgcgcaa ctgctcgccg taggcgcgta
      841 tgtagtcgct ggcgaagacc accgggccct ggcggccacg caactgggtg gcaaggtaac
      901 tctggcgcgt cttcttggtc gggtgcagct gattccagcg tgcgcagtcg cgcgcctcgc
      961 gggccagttc gttgacgctg gtcgcgctcc agatgtcggc attgaccttg aaatcccgtt
     1021 tcagcaaatc cgccgcggcg atgacttcgc gcagtatcgt gcccgagccc atcagttgca
     1081 gcttgggccc cttttcgtcg ccctcgtcga gacagtaaag gcccttgatg ataccgtcct
     1141 cgcagccttt cggcatgctc gggtggctgt aattctcgtt cattacggtg atgtaataga
     1201 acaccttctc gcgcatttgg tacatgcgct tgagcccgct gtgaatgatc accgcgagct
     1261 cgtaggcgaa ggtcgggtcg taggaaatgc agttcggaat ggtgctggcg acgatcaggc
     1321 tgtggccgtc ctggtgttgc agaccctcac cggccagcgt ggtgcgcccg gcggtgccgc
     1381 ccatcaggaa gccgcgcacc tgcatgtcgc cggccgccca ggcgaggtcg ccgatacgct
     1441 gaaaaccgaa catcgagtaa aaaatataga atggaatcat gttcacgccg tgcgtgctgt
     1501 aggcgctgcc ggcggcaatc cacgacgaca tcgcgccggc ctcgttgatg ccttcctcga
     1561 gaatctgccc ggcgacgtcc tcgcggtaat acatcacctg gtcggcatcc atcggcttgt
     1621 atagctgccc ctcgaaggca tagattccaa gctggcggaa catgccctcc atgccgaagg
     1681 tgcgcgcctc gtcggggacg atcggcacga tgttcttgcc gatgttcttg tcgcgcgcca
     1741 gcgtcgacag catgcgcacg aaagccatgg tggtcgaaaa ctcgcgatcg ccgctgtcct
     1801 tgagcagcga atcgaagatt tcgagccccg gcacgatcag cgcttccgcg ttatcgcggc
     1861 gaaccggcat gaagccgccg agctgctcgc ggcgttcgcg catgtatttc atctcctcgg
     1921 tgtcgtcggc aaacttgtag aacggcgcgt cggcgatgtc ttcgtcgctg atcggaatat
     1981 tgaagcggtc gcgaaacgcc ttcaacgcct cctcgcccat tttcttttgc gagtgggtga
     2041 tgttctgccc ctcgccggcc tcgcccatac cgtaaccctt gacggtcttg gcgaggatga
     2101 ccgtcggccg ccccttgctc tcgaccgcct cggcataggc cgcgtagacc ttgtgcgggt
     2161 cgtggccgcc acgattgagg cgccagatgt cctcgtcgga catgtcggcg accatatcgt
     2221 gggtctcggg gtacttgccg aaaaaatact cgcgcgtgta ggcgccgccc ttggctttgc
     2281 aggcctggta ctcgccgtcg acggtctctt ccatcagccg ctgcagggta ccgttgttgt
     2341 ccttggcgaa cagttggtcc cagtacgagc cccagacgac cttgagcacg ttccagccgg
     2401 cgccacggaa tacggcttcg agttcctgga tgatcttgcc gttgccgcgc accggtccgt
     2461 cgaggcgctg caggttgcag ttgatgacga agatcaggtt gtcgaggtct tcacgcgccg
     2521 ccagcgaaat tgcgcccagc gattcgggct cgtccatttc gccgtcgccc atgaacgccc
     2581 aaaccttgcg gtcgtcctgc tgctggatgc cgcggttctc gaggtatttc atgaatctcg
     2641 cctggtagat cgccatgatc ggcccgaggc ccatcgatac ggtcgggaac tgccagaagt
     2701 tcggcatcag ccacgggtga ggataggaag acaggccgtt ttcattcaac gcctcctggc
     2761 gaaacgattt cagctgctct tcgctgatgc gcccctcgag aaacgcgcgc gcgtagattc
     2821 cgggcgccga gtggccctga aaaaatacca ggtcgccgtt gttttcgtcg ctcggtgcac
     2881 gaaaaaaatg catgaaaccg acgtcgtaga gggtcgcggc cgacgcgaag ctggcgatat
     2941 gaccgccgag ttcgctcgat ttacggttgg cgtgcacgac catcgccgcc gcgttccagc
     3001 gaatcaccga gcgaatactg tgctcgatcg cctggtcgcc ggggatcggc ctttgctgcg
     3061 acagcggaat ggtattgacg taagcagtag tcgccgagaa aggcaggtag gcgccggaac
     3121 ggcgggtttt atcgatcaac ttctccagca ggtagtgcgc acgttcgggc ccctcgcgct
     3181 cgagtaccga atccagcgac tctatccatt cctgggtttc ctgcggatca atgtcagttt
     3241 catctaccac gtgcattgcc tttgttgtaa attggcggga ttatacagca attaaattta
     3301 cgctcagggc ggtcgatcat tttcatacca gtatttttat gcctatatta atctccgcac
     3361 cttccatact gcaataaatg aagcgcctcg acatcgtcaa acccgatgac tggcatctgc
     3421 acctgcgcga caccccgtat ctcgaagccg tggtcggcga taccgcgcga cagttcggcc
     3481 gcgccatcgt gatgcccaac ctttcaccgc cggtgcgcag ctgtgccgat gcgatggcgt
     3541 accgcgagcg cattctcgcg gcactgccgc cgcacagcga attcgaaccg ctgatgaccc
     3601 tctacctgac cgacaacacg tctgccgagg agatcgccgc ggctgccgag tcgccattca
     3661 ttcacggcgc caaactctac ccggccggcg cgacgacgaa ttccgcagcc ggggtcaccg
     3721 atctggatag catctggccg gttttcgagg ccatgcagcg gcatcgactg atcctgcagg
     3781 tccacggcga ggctaccggt gagcagatcg acgttttcga tcgcgaaagg gttttcatcg
     3841 agcaaagtct cgagcgcatt cgccgcgatt ttcccgagct gcgcatcgtt ttcgaacata
     3901 tcaccaccag agacgcggcc gcctgggtgc gcgacgcggg gcccggcctg gcggcgacca
     3961 tcaccccgca tcacctgctg atcaatcgca acgcgctgtt cgcgggcggc ttgagaccac
     4021 accattactg cctgccggtc gccaagcgcg aacatcaccg ccaggcgttg ctcgccgctg
     4081 ccaccggcgg cgaggcctgc ttttttgccg gcaccgacag tgcgcctcac gcccgcgacg
     4141 ccaaggaatc tgcctgcggc tgtgccggca tctactcggc gcacagcgcc ctcgaactgt
     4201 acgccgaggc attcgacgaa gccggggact tcagccattt cgaagccttc at
//
LOCUS       DATCZV010000348         4622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_134234, whole genome shotgun sequence.
ACCESSION   DATCZV010000348 DATCZV010000000
VERSION     DATCZV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134234"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..664)
                     /locus_tag="VIW27_07805"
     CDS             complement(371..664)
                     /locus_tag="VIW27_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739615.1"
                     /translation="MHRSNLRQICLILVALAVAPAASAESWICEHSTLVREISVERAT
                     AEPAPCSVAYNKQSEGQGVKILWSAQNDGSYCDAKADGLAEKLQGMGWSCTAF"
     gene            943..1953
                     /locus_tag="VIW27_07810"
     CDS             943..1953
                     /locus_tag="VIW27_07810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5739616.1"
                     /translation="MHRRFFLVTVLLALPVLEGRCAEALLVPSAALVDEIFRVGPIKS
                     FMVQRDGELLVDAHRMGMRAERSTNVKSVAKSVISLLVGIAIEQGYLRGVQQPIGEFF
                     PDYFRRRPDPAKQAITIQDLLTMRAGLASTSRRNYGRWVTSDNWVEHVLDQPLVGQPG
                     GEMIYSTGSSHLLSVILTRATGLSTREYARRHLFGPLGITDGGWDQDPQGYYLGGNNL
                     ALSPSALLKIGTMVINLGVYDGKQIVPRDWIVESMRIYTRSVFNPYDYGYLWWQRELN
                     GYTVQFAWGSGGQYLMIFPALEAVVAITSHAEQGVQQSRQSRRRLFEFIENRLIGYLQ
                     QS"
     gene            complement(2054..2236)
                     /locus_tag="VIW27_07815"
     CDS             complement(2054..2236)
                     /locus_tag="VIW27_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739617.1"
                     /translation="MSKFVLDEETLRDFQEGLEDIKKMKQNPADEVTRKRFEKKWENH
                     DARELLELFMSPDSED"
     gene            complement(2529..3365)
                     /locus_tag="VIW27_07820"
     CDS             complement(2529..3365)
                     /locus_tag="VIW27_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEY5739618.1"
                     /translation="MIRNKHLASAAAALLLVGIATLAAAAPRAELWERWSAHDPDSAQ
                     RIDHRPRNEILSRFVVPASDGINRFAYSRVGAADRQLLRDYLQAMSRVEIGSYRRDEQ
                     RAFWINLYNALTVEVILEHYPLATIRDISSGFFSRGPWDLELIEIDNQALTLNDIEHR
                     ILRPIWQDPRIHYAVNCASLGCPNLQRQAFTAANSDSLLEVAAVEFVNHPRGARVVDG
                     ELLVSSIYDWFEADFGGNEAGVIAHLRRYAKAELRAALAGIDDIADDNYDWSINSLQP
                     PR"
     gene            complement(3557..3904)
                     /locus_tag="VIW27_07825"
     CDS             complement(3557..3904)
                     /locus_tag="VIW27_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739619.1"
                     /translation="MKPVTIEFTVREGDNSFKEDSISFASPEEMFAYISPGGGCDDLP
                     SDLAEIRMIFLPPKHPNITNPVADMRATLQLGIVLITAPLSQIGQICGEIIDKVGRAE
                     ISEAFLAAVRPNC"
     gene            complement(4095..>4622)
                     /locus_tag="VIW27_07830"
     CDS             complement(4095..>4622)
                     /locus_tag="VIW27_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739620.1"
                     /translation="IFEQHCSGLAREFDGARPYPSTGRWQSIIAQQGFEIGRVLQHLG
                     YFGRCSFDAILVGTDDSDAQLHWVECNGRWGGVSLPLTLNRRLDRGRTASMPFVVCEQ
                     AHQQLPGRHLRSVIETLEPWLYRSGLRDDGVILLSPGRLFDGSGFELMSRAQDIDGAA
                     QTAARASALLSGAVS"
BASE COUNT          853 a         1388 c         1455 g          926 t
ORIGIN      
        1 gatgtgccct aacagcgaag taagtgtcag ttgcgccaag agcagcggga tcgtcaaaaa
       61 atgggactgc agtacgccga ccaatcgagc ggtccatcaa aatctctacg aggcggtcag
      121 gcaggcctgc aggtagtgac agcggtgatc caccttttat cggctgctcg ttcagggtaa
      181 ccggccgtca ccgacaggca cccgtcggcc agcgattttt ttctgtctcg cggcgttcag
      241 gaatcgggat ccggcccgtc ggaggatgcg ggcagggcga tcggccgccc gcaagactgc
      301 gctgaacgcg ggcagggcgg cgatcaagat ggcgaaatca acttgctcgc cttgccgatg
      361 gccggctcga ctagaaggcg gtgcaactcc agcccattcc ctgcagtttc tcggcgaggc
      421 cgtcggcctt cgcatcgcag taagagccat cgttttgcgc gctccacagg atcttgacgc
      481 cctgtccctc ggattgcttg ttgtaggcta ccgagcaggg tgccggttcg gcggtcgcgc
      541 gctcgacact gatttcgcga accagggtgc tgtgttcaca gatccaggat tcggcgctgg
      601 cggcgggcgc cacggccagc gcgaccagga tcaggcagat ttggcgaaga ttcgatcgat
      661 gcatgggttt cgagctcctg tgcaagtttg tgcatgggcc agccctactg gccgttcagt
      721 tagagcatag tcgctgtaaa cgcggccggt gggaataaat gacgcgcgcg aacgggcgta
      781 ccgccaccgg cttggccagc ccggggtggc gcagtgcaaa acggccggaa aaaatcgccg
      841 attcgccgta cctgttggcg gcaaggattg ccgggaaccg acctcgattc gagtatactt
      901 ttcggcgata tcgttcagga cccgctaaca cggatttaac cgatgcaccg ccgttttttt
      961 ctagtgactg ttttgctcgc gttgccggtg ctcgaaggcc gctgcgcgga agccctgctg
     1021 gtaccgagcg ctgccctcgt cgacgagatc tttcgcgtcg gcccgatcaa gagtttcatg
     1081 gtgcagcgag acggggaact gctggtcgac gcgcaccgca tgggcatgcg tgccgagcgc
     1141 tcgaccaacg tcaagtcggt ggcgaagagc gtcatctcgc tgctggtcgg catcgccatc
     1201 gagcaggggt acctgcgagg cgtgcagcaa ccgatcggcg aatttttccc ggactatttc
     1261 aggcgccggc ccgatcccgc aaaacaggcg atcacgattc aggatttgct caccatgagg
     1321 gctggcctgg catcgaccag ccgccgcaac tacggtcgct gggtaaccag cgacaactgg
     1381 gtcgagcacg ttctcgatca gccgctcgtc gggcagccgg gcggggagat gatctacagc
     1441 accggctcct cgcatttgct atcggtgata ttgacgcgag cgacggggct cagtacccgc
     1501 gagtatgccc gacgccacct gttcggcccg ctgggtatca ccgacggcgg ctgggaccag
     1561 gacccgcagg gctattatct cggcgggaac aacctcgcgc tgtcaccgtc ggcgctgctc
     1621 aaaatcggca cgatggtgat caacctcggg gtttacgacg gcaagcagat cgtgccgaga
     1681 gactggatcg tcgagtcgat gcgcatctat acccgcagcg tattcaatcc ctacgactat
     1741 ggctacctgt ggtggcaacg cgagttgaac ggctacaccg tgcaattcgc ctgggggagc
     1801 ggcggccagt acctgatgat atttccggca ctggaggccg tggttgcgat cacatcccac
     1861 gcggaacagg gtgtgcagca aagccggcaa agccggcgcc ggctgttcga atttatcgaa
     1921 aaccgcctga tcggttacct gcaacaatcc tgatcgacgc cgggcaggcc ggcgcgcgtc
     1981 catcgggatc tgccgccccg aaagcagtta ttgcgtgcgc gaatccgtgc tggcggatta
     2041 tcgccgcggg attttagtct tccgaatccg gggacatgaa caattccagg agttcccgcg
     2101 cgtcgtgatt ctcccatttc ttctcgaacc gcttgcgggt gacctcgtcc gcgggattct
     2161 gtttcatttt ttttatgtct tccagacctt cctgaaagtc gcgaagcgtc tcttcgtcga
     2221 gcacgaattt actcatcgct agcccgtttc acgttgcccc ctcccaaaac tgtcacgtcg
     2281 gcggtgcgag ccggttacct ctggatccgt tccgtcatcg gccgcgcgca cgcgtggatt
     2341 atgcaaaact agcaatcccg acgtatcggc gcatcgccca aatggggtaa tttcaatgcc
     2401 gcggggtttt gcgaagcgtg gcgtcgggcg cggtaattta cggattttca ggctaccccg
     2461 atcaatccag ggcccggtcc gcggctcccg gctcgagctc gtcgacagcg ctcaggcgca
     2521 gattcaggct agcgcggtgg ctgcagggaa ttgatgctcc agtcgtaatt gtcgtcggcg
     2581 atatcgtcga tgccggcgag ggcagcccgc agctccgctt ttgcataccg tctcaagtgc
     2641 gcgatgacgc cggcttcgtt gccgccgaaa tccgcctcga accagtcgta gatactcgac
     2701 accagcagtt caccgtcgac cactcgcgcg ccgcgcggat ggttgacgaa ttcgaccgcg
     2761 gcgacctcca gcaggctgtc gctattggcg gccgtaaagg cttgccgctg caggttgggg
     2821 cagcccagcg atgcgcagtt aaccgcgtag tgaatgcgcg ggtcctgcca gatcggccgc
     2881 aggatgcgat gctcgatatc atttagcgtc agcgcctggt tgtcgatctc gatcagctcc
     2941 aggtcccagg ggccgcggct gaaaaagccg gacgagatat cacgaatcgt cgccagtgga
     3001 taatgctcga gaatgacctc gacggtgagc gcgttgtaga ggttgatcca gaaagcgcgc
     3061 tgctcgtcgc gccgatagct gccgatctcg actcgagaca tggcttgcag gtaatcccgc
     3121 agcagttgcc ggtcggcggc acccacacgg ctataggcaa agcgattgat accgtcgctc
     3181 gccggcacca cgaagcggct gagtatctcg ttccggggcc ggtgatcgat gcgctgtgcg
     3241 gagtcgggat cgtgcgcgct ccagcgctcc cagagttcgg cgcgtggcgc ggccgccgcc
     3301 agcgtcgcga tgccgactaa cagcagtgcg gcggcggcac tggccagatg tttgtttcga
     3361 atcatgcttg tgatcgggtg tttacagcag cgtgttggac tcgatcggga cagtttcgtt
     3421 caacccgcct ggcggcggcg gtgggattgc gcgtgacagt atacctgcag cgtcgcggtg
     3481 atcgtgaatc ggcggttagg cttccgagcc tgcccgcggc cggctgcgat gaccggcgca
     3541 cggcgcatcg aatggctcag cagttaggcc tgaccgccgc caggaaggct tcggagattt
     3601 cggcgcgtcc caccttgtcg ataatttcgc cgcagatttg cccgatctgc gacaacggcg
     3661 cggtgatcaa tacgatcccc agttgcaggg tggcgcgcat gtccgcgacc ggattggtga
     3721 tattcggatg cttcggcggc aggaatatca tgcgaatctc tgccagatcg gacggcaggt
     3781 catcgcagcc gccgcccggg gagatatacg cgaacatctc ttccgggctg gcaaaagaga
     3841 tactgtcttc cttgaacgag ttgtcacctt ctctaaccgt gaattcgata gttacaggct
     3901 tcatgacctt caccgctgat tatctggctt taactctact ccgcgtaccc ggtatcggcc
     3961 ttgtcttgag tcaaatagtc ggcccgtcgg cgagcggggg acgatcgagc cactcgatcg
     4021 actggcgcga aatgcgaggc cggtttgcgc gcgatgttgg tcgcaagccg ggattccggt
     4081 gatgaagcct ggagtcagct gacggccccg cttagcaggg cggaggcccg ggcggcggtt
     4141 tgcgcggcgc cgtcgatgtc ttgcgcgcgc gacatcaact cgaatccgct gccgtcgaac
     4201 aatcttccgg gcgataacag gatcacgccg tcgtcgcgca ggccgctgcg gtacagccag
     4261 ggttcgagag tctcgatcac cgaccgcaag tggcgcccgg gtaattgctg atgggcctgt
     4321 tcgcagacca cgaacggcat cgatgccgtt cgcccccgat cgaggcgccg attcagcgtt
     4381 agcggcagcg aaacgccacc ccagcgaccg ttgcattcga cccagtgcaa ctgcgcatcg
     4441 ctgtcgtcgg tgccgacgag aatcgcatcg aaactgcagc ggccgaaata accgagatgt
     4501 tgcaataccc tgccgatttc gaatccctgc tgcgcgatta tcgattgcca gcggccggtc
     4561 gacgggtagg gtcgggcacc gtcgaactct cgtgccaggc cgctgcagtg ctgctcgaag
     4621 at
//
LOCUS       DATCZV010000349         5985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_134270, whole genome shotgun sequence.
ACCESSION   DATCZV010000349 DATCZV010000000
VERSION     DATCZV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134270"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..595
                     /locus_tag="VIW27_07835"
     CDS             293..595
                     /locus_tag="VIW27_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY5739621.1"
                     /translation="MRKSSRRRFVKFAATAVALSPSLLQLGRPVMAAEMPKVDPDDPQ
                     ARGLSYVHDSPKPESLCANCMLYTGSAESEWGPCAVFPGKLVAADGWCSAWVKKGG"
     gene            988..1302
                     /locus_tag="VIW27_07840"
     CDS             988..1302
                     /locus_tag="VIW27_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739622.1"
                     /translation="MIKQIQMTWKSVMDHRYNPLRFLDVRSGHYLMQVLSWMWSMIFS
                     LSFLSIYQFGYVWAAHLLVIGGVFVTVSIFERSKASREKLAPAPYLSRGANTVWQMDR
                     EA"
     gene            complement(1765..3690)
                     /locus_tag="VIW27_07845"
     CDS             complement(1765..3690)
                     /locus_tag="VIW27_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5739623.1"
                     /translation="MNFFEAQDKARRSTFWLVLLFALAVIALILLTNLFLLIVYRYLQ
                     TSEIVVSPETLYQIYPAETFVAVGIGVVLLILGGSLYKTLSLAGGGPTVAEMLGGHLV
                     SQGTSDPMHRRLLNVVEEMALAAGMPIPQVYVLADSSINAFAAGRTPADAVIGITDGA
                     LRRLRRDELQAVIAHEFSHIANGDMRLNIRLIAILHGILLIGLIGYFLLRSLRHTGRS
                     RSRKGGGGLLAIAMLGAGLMVIGYAGSFCGQWIKAIVSRQREYLADSSAVQFTRNTDG
                     IAGALKKIGGSAGVGSYLDSSSAPQYSHAYFADGISSFWQSMFATHPPLGDRIRRIEP
                     GWDGEFLPAEILQAIEPEREQQPQPVSGAAMVAAAVLTSAQQAISEVGTLNEQNIDYV
                     RELIAVMPAALREAAQDPYSARALIYAGLVQIQRDETAALESLAAAIANPEMQALVEK
                     FAPLLQQLDDRLKLPLLELCVHSLREMSPNQYVQFKRAVHNIITADRKVSLREWVVQR
                     FVIQQLDLHFGFRGAPRAKHGSLQAVRDDVAIILSVIAHAEHRDDAEVRAAFAAGAAE
                     TALDDLQPVMPMSIGLERLDVALDELMRLKPLVKPRLLKACVAIILYDGRTTPRGIEL
                     VRAISTCIDCPMPPMRP"
     gene            complement(3713..4309)
                     /locus_tag="VIW27_07850"
     CDS             complement(3713..4309)
                     /locus_tag="VIW27_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5739624.1"
                     /translation="MEVSTIVVLVIIGAIVLYIIAIFNKLVALSNRYKNAFSQIEVQL
                     KRRYDLIPNLVETAKGYLKHERETLDAVIKARNSASDRLSAAQQKPGDPATMQGLGQA
                     EGNLAGALGRFNLLVEAYPDLKANQNMMQLNEELTSTENKVAFARQAFNDQVMAYNIY
                     KQSFPQNLFAGMFGHALDASLLEFEDSAAIQEAPKVSF"
     gene            4814..5029
                     /locus_tag="VIW27_07855"
     CDS             4814..5029
                     /locus_tag="VIW27_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017021019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02450 family Trp-rich protein"
                     /protein_id="HEY5739625.1"
                     /translation="MNPINPRKLLNSKWTAVNPARKEKHFVVTDVVFDEDGAVLHCLI
                     EAVISRRAERIDWRELKNREHWLQGWK"
     gene            complement(5125..>5985)
                     /locus_tag="VIW27_07860"
     CDS             complement(5125..>5985)
                     /locus_tag="VIW27_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739626.1"
                     /translation="VMVPLILYLGPPHRILTPLWPLHLARAMLFAFGFSLYYTAFPFM
                     TLAAVTTIFFAAPLITAVFAAVFLKEHIGIHRISALVLGFAGVIIAMKPGSDSFHWIS
                     VLPLICACSYATSQMLARKIGHRETAMVAGAYTIILAGLLVLPLGWTLNALFDIGAQA
                     AHLRWSWEAFSFGILPWLALLGALGMVGWTLLSRAYQIADVGAVAPFEYAYLPLAAAL
                     GYLAFDEVPAWNTLLGMALIIVSGIYIGYREVVNARDSIKPTAEASFAPGNPGVIPEA
                     HEVDSPPARQ"
BASE COUNT         1193 a         1770 c         1884 g         1138 t
ORIGIN      
        1 atcgcattct acgaatcgct cggcttcgtc gtcacgcgcc gtgcgccgat cgtcgaacac
       61 ccgggcatca gggcgagcgg cgatgcggtg ctgatggaaa ccaggccgcg gtaatcgcga
      121 ccggcgaggc cggcaagcga attcaggatc accgaattca ggggcgagcc ttggatttca
      181 tatctccggg ttcaaaattg ctagttttcg attatttgtc taattaatga ttatttttaa
      241 tctatacaat aagtagacgc gtatacagtt tactgcaatt atggagtaaa ccatgcgcaa
      301 gtcgagccgc cgtcgattcg tcaagttcgc agcgaccgcc gttgccctga gtccatcgct
      361 gttgcagctc ggccggccgg tcatggcggc cgaaatgccg aaagtcgatc ccgacgatcc
      421 ccaggccagg ggcctgtcct acgtacacga ttcgccgaag cccgaaagcc tgtgcgccaa
      481 ttgcatgctc tataccggca gcgcggaatc ggaatggggc ccctgtgcgg tctttcccgg
      541 gaaactggtc gccgccgacg gctggtgcag tgcgtgggtc aaaaagggcg gttaggcgcc
      601 tgttcgcacc catcgagccg actctgattt caggagacag acatggaaaa cgatatcgcc
      661 tttatggccg ctggatacgt tctggcccgg atcgggatac tggtagcctt tggctacctg
      721 gtttatcgca tagtacgccc cgagccggcc cgaattcgtg ccaaaagtaa atcgagcact
      781 gcgcgagcag gctccggtcg gatccgcatg gaccgctaaa gaatacgtct ccgtcggcac
      841 ctcccctccc gcttcgtcag ccgcaagatg tggctgcggc cggtgccgac ggatgacgtt
      901 ctctcgagtc gactcccgcg agggtgtcgg cggggagggg tgggacacgc agaggtcccg
      961 aaacattcaa ttgttggagg aaggattatg atcaagcaaa tacaaatgac ctggaaatca
     1021 gtcatggacc atcgctacaa cccgctcagg tttctcgacg tgcgctcggg acattacctg
     1081 atgcaggtgc tgtcgtggat gtggagcatg attttttcgt tgtcattctt gtccatttac
     1141 cagttcggtt acgtctgggc cgcgcatctg ctggtgatcg gcggggtatt cgttacggtg
     1201 tcgatcttcg agcgctcgaa agcctcgcgc gaaaaactgg caccggcgcc ttacctgagc
     1261 cgtggcgcca acacggtatg gcaaatggat cgcgaggctt aggcgactgc tgcccgatta
     1321 accgcatgac gggaccaggc cctgcccgtg ccgcctggag ggtgggtatg gcgggaagga
     1381 agtccaaccc tctcgcagga agcgaacgag ggtggcagcc gatccgagcc agcaagagat
     1441 tcggggggca gattacgcgc tgcagaccat cagccggcgt ctgcaagtaa gtaagctgtc
     1501 ccccgatacc tgtttttcgg tcttcgcaga tggtggcagt tgaggccttg cctctcacct
     1561 tgcaggtgac ggccccatcc aaaagccaat atgccgcgct aatctgctgc tcgccgccgc
     1621 ggtaaatcag acgacgtcta ttccgtcagg ccggccacag ccgcgctccc gtcatcgtcc
     1681 gttcctgggg gctaccagat tagcctgtgg attcgatggc agacggcgat tagtttgcac
     1741 agtctgagct gacgaggatt gcactcaggg ccgcatcggc ggcatcgggc aatcgataca
     1801 ggtcgagatc gcgcgcacca gttcgatgcc gcgcggcgtg gtgcggccgt cgtagagaat
     1861 gatcgcgacg caggccttca acaggcgtgg cttgaccagc ggcttgaggc gcatcagttc
     1921 gtcgagcgcc acgtccaggc gttcgagccc gatcgacatc ggcatcaccg gttgcagatc
     1981 gtcgagcgcg gtttccgcgg cgcccgccgc gaaagccgcg cgcacttcag cgtcgtcgcg
     2041 gtgctccgcg tgcgcgatca ctgacaggat gattgcgaca tcgtcgcgca ccgcctgcag
     2101 gctgccgtgt ttcgcccgcg gcgcgccgcg aaagccgaaa tgcagatcca gttgctggat
     2161 gacgaagcgc tgcaccaccc actcgcgcag gctgaccttg cggtcggcgg tgatgatatt
     2221 gtgcaccgcg cgtttgaatt gcacgtactg gttgggcgac atctccctca gcgaatgcac
     2281 gcacagctcc agcagcggca gcttcaggcg gtcatcgagt tgctgcagta acggtgcgaa
     2341 tttctcgacc agtgcctgca tttccgggtt ggcgatcgct gccgccagcg actccagcgc
     2401 cgccgtctcg tccctttgaa tctgcaccag cccggcataa atcagggcgc gggcgctata
     2461 gggatcctgc gcggcttcgc gcagcgccgc cggcatgaca gcgatgagct cgcgcacgta
     2521 atcgatgttt tgctcgttga gcgtgccgac ttcgctgatc gcctgttgcg cggaagtcag
     2581 cactgcagcg gcgaccatgg ctgcaccgga aaccggctgc ggctgctgtt cgcgttcggg
     2641 ctcgatcgcc tgcaggatct ccgccggcag gaactcgccg tcccagccgg gctcgatgcg
     2701 cctgatgcgg tcgccgagcg gcggatgcgt ggcgaacatc gattgccaga agctcgagat
     2761 accgtcggcg aagtaggcgt ggctgtactg cggcgcggat gacgaatcca ggtaggaacc
     2821 gacgcccgcg gaaccgccta ttttcttcag cgcaccggcg atgccatcgg tattgcgggt
     2881 aaactgcacc gccgacgaat cggccaggta ctcgcgctga cggctgacga tcgccttgat
     2941 ccactggccg caaaagctgc cggcataacc gatgaccatc aggccggcgc caagcatggc
     3001 gatcgccagc agtccgccgc cgcccttgcg gctgcgcgag cggcccgtat ggcgcagcga
     3061 gcgcaacagg aaataaccga tcaggccgat cagcagaatg ccgtgcagaa tcgcaatcaa
     3121 acgtatgttg aggcgcatat cgccgttggc gatatgactg aattcgtggg cgatgacagc
     3181 ctgcaattca tcgcgcctga gccgcctgag cgcgccatcg gtgatcccga tgacggcatc
     3241 ggccggcgtg cgcccggcgg cgaaggcatt gatcgagctg tcggcgagca cgtagacctg
     3301 cggaatcggc atgcccgccg ccagcgccat ttcctcgacc acgttgagca ggcgccgatg
     3361 catcggatcg ctggtgccct gcgaaaccag gtggccgccg agcatttcgg cgacggtcgg
     3421 cccgccgccg gcgagcgata acgtcttgta caggctgccg ccgagaatca gcaacaccac
     3481 gccgatgccg acggcgacga aggtttctgc gggatagatc tggtacagcg tttccggcga
     3541 gaccacgatc tcgctggttt gcaggtagcg gtagacgatg agcaggaaca ggttggtcaa
     3601 caggatcagc gcgatcaccg cgagtgcgaa cagcagcacc agccagaagg tgctgcggcg
     3661 ggccttgtcc tgggcttcga agaagttcaa cttgcggcgg tttcggcgcg gcctagaaag
     3721 agactttcgg ggcctcctgg atcgcggcgc tgtcctcgaa ctcgagcagg ctggcgtcga
     3781 gcgcgtggcc gaacatgccg gcgaacaggt tttgcggaaa agactgttta tagatgttgt
     3841 aagccatcac ctggtcgttg aaagcctggc gcgcgaaggc gaccttgttt tcggtcgaag
     3901 tcagttcctc gttgagctgc atcatgttct ggttggcttt caggtcggga taagcctcca
     3961 ccagcaggtt gaagcggccc agcgcgccgg ccaggttgcc ctcggcctgg ccgaggccct
     4021 gcatcgtcgc cgggtcgccc ggtttttgtt gtgccgccga cagtcgatcg gaagccgagt
     4081 tgcgcgcctt gatcaccgcg tcgagggttt cgcgctcgtg cttgaggtaa cccttggcgg
     4141 tttcgaccag gtttggaatc aggtcgtaac gccgcttgag ctgcacctcg atctgggaaa
     4201 acgcattctt gtagcgattg gagagcgcca ccagcttgtt gaatattgcg atgatataca
     4261 acacgatcgc gccgatgatg acgagcacaa cgatggtcga tacttccata tttcctccag
     4321 cctgtgaatc gattgcggag cagcggccct ggctccgctg cgcgagccga taccagctgc
     4381 ctgccccgta ctccttgcat agcaaagata gcagtaatat gcagcgaatg cgcggatttc
     4441 gcccgccgcg cgatcgatgg ctgaagccgc tagcgagcgg gccacgttcg gctgaggatg
     4501 gcatcgccgg ctcgatagca gatccggcta ttctaaagat atcgatcgat tcttttctag
     4561 gccgaacttc ggcccatgta aaacaccacc gacagaatca tcagcgccac gcttgcgccg
     4621 atatatttgc catacggatc atcgagtagg aaaattgggg gtcgggccca caatcttacg
     4681 agtgtagaaa aaccaaatac ggaatgtacg gcctgacctc aattctcatg tgtgccgatg
     4741 tgtgccgcgg cgatcccggg ctgatcgggt tgactgtcga ttgctgacaa ctccctacag
     4801 tggtatggcg cgagtgaacc cgatcaatcc tcgaaagtta ctcaacagca agtggaccgc
     4861 ggtcaatcct gcccgcaagg aaaagcactt cgtggtcacc gatgtcgtat ttgacgagga
     4921 cggtgcggtc ctgcattgcc tgatcgaggc ggtgatttcc cgtcgtgccg agcgaatcga
     4981 ctggcgagaa ctgaaaaacc gggagcactg gctgcagggc tggaagtaaa caccgcgcga
     5041 agatacggga tcagtcctca ccattcacat cggctactcg gagcagggga aaggtaaggc
     5101 ccgatccttt tcatgcgcgg cgcttcactg ccgagctggc ggcgaatcga cctcgtgggc
     5161 ctcgggtatg acgccgggat tgccgggggc aaagctcgcc tcggccgtcg gtttgatgct
     5221 gtcgcgggca ttgacgactt cgcggtagcc gatgtagatc ccggagacga tgatcagcgc
     5281 catgccgagc agcgtattcc acgccggaac ctcgtcgaaa gcgaggtaac cgagtgccgc
     5341 cgccagcggc agataggcat attcgaacgg cgccacggcg ccgacatcgg caatctggta
     5401 ggcccgcgat aacagcgtcc aaccgaccat gccgagggcg cccagtaaag cgagccaggg
     5461 caggatgccg aagctaaagg cttcccagct ccagcgcagg tgcgcggcct gcgcgccgat
     5521 atcgaacagg gcgttgagcg tccagccgag cggcaacacc agtaatcccg cgaggatgat
     5581 ggtgtaggcg cccgcgacca tggccgtttc gcggtggccg atctttcgcg ccagcatctg
     5641 cgatgtcgcg tagctgcagg cgcagatcag cggcagcacg gagatccagt ggaacgagtc
     5701 cgagcccggt ttcatcgcga tgatgacgcc ggcgaaaccg agcaccagcg cgctgatccg
     5761 atggatgccg atgtgctcct tcagaaacac cgcggcaaat accgccgtga tcagcggcgc
     5821 ggcgaagaat atcgtcgtca ccgcggccag ggtcatgaac gggaacgccg tgtagtagag
     5881 cgaaaagccg aaagcgaaca gcatggcgcg cgcgaggtgc agcggccaaa gcggcgtcag
     5941 gatgcggtgt ggaggcccga gatacaggat cagcggcacc atgac
//
LOCUS       DATCZV010000350         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_240431, whole genome shotgun sequence.
ACCESSION   DATCZV010000350 DATCZV010000000
VERSION     DATCZV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240431"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VIW27_07865"
     CDS             complement(<1..613)
                     /locus_tag="VIW27_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY5739627.1"
                     /translation="MATMSILLALLDLQQLPLSLLFTLEALWEMLPFFALAIGIAAYA
                     RATSADSLIAKAFSGHPVRATMLAALVGAISPFCSCGVIPLIAAMLGAGVPLAPVMAF
                     WIASPIMDPEMFILTAAGIGFNFAVAKTVAAMAMGLMAGLSILLIQRYGGLESPLRAE
                     VTGCCDSSCSTSTEKAVLWRFWREPGRIEVFNAESIAIGVFLGK"
     gene            complement(736..1044)
                     /locus_tag="VIW27_07870"
     CDS             complement(736..1044)
                     /locus_tag="VIW27_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5739628.1"
                     /translation="MKLDTAANILAKIGNPTRLRIVRLLVRTGDEGLPVGVIQKKLGI
                     PGSTLTHHITHLKSAGVIRQQRRQATLICRVEFGVIQGLVDYLTAECCADIDSSETAA
                     "
     gene            complement(1163..2470)
                     /locus_tag="VIW27_07875"
     CDS             complement(1163..2470)
                     /locus_tag="VIW27_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY5739629.1"
                     /translation="MSLTLDLIGLIRKRPVSTTDLDDAALFTLDAVATAYAGSSTPVG
                     AILRGWATDGDLDLKRKALLMGALTHITETDDLHRASVTHPGCVVVPAVLALGEKTGA
                     SGEQMLRAILHGFEAMCRIGAAVGPAHYRIWHNTATCGPFGSAMAAASLLELTDEQTL
                     NALGNAGTQSSGLWQFMETGAMSKHLHAGRASESGLLAAELAAHGFTGPAEILEGSKG
                     LFAGTCDDPIPENILRQPDDKWQLSLTSIKPWPSCRHTHPIIDCALEIHQLLDGKPTR
                     EVGIKTYQAALDVCDCREPETEYQAKFSLYHTAAIALTDGVVGLDSFDADARERSARL
                     RRATSVSIVDPYASNYPVSWGAEVTAVTESGEAFKVSRKDCKGDPELALDRSEMRAKA
                     TGLLQYGGLDQAAAIRVCDEVLALPTMQNNPGLFTRFLDHISR"
     gene            2549..>2830
                     /locus_tag="VIW27_07880"
     CDS             2549..>2830
                     /locus_tag="VIW27_07880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5739630.1"
                     /translation="MSAKQVIGGPLEIDGRVLSLSKAVRAGDFVFVTGQVPMRDGKPI
                     TDGSIEDQTRACLDGLSEILAEAGCELGDVVKSMVWLKKREDFPGFNSVY"
BASE COUNT          566 a          816 c          896 g          552 t
ORIGIN      
        1 atttaccgag aaacacgccg atcgcaatgg attccgcgtt gaaaacctcg atgcgcccgg
       61 gctcccgcca gaatctccac agaaccgctt tctcggtgga agtcgagcag ctgctgtcgc
      121 agcaaccagt cacctcggcg cgcagcggcg attcgagccc gccgtagcgc tggattagca
      181 ggatagacag gccggccatc aatcccattg ccatcgcggc gacagttttg gcaaccgcga
      241 agttaaagcc gatgccggcg gcggtaagga tgaacatttc cgggtccatg ataggtgacg
      301 cgatccagaa agccatgacc ggtgccagcg ggacgccggc tcccagcatc gcggcaatca
      361 gcggaatgac gccgcaggag cagaacggag aaatagcacc gaccagtgcc gccagcatgg
      421 tcgcccgcac cgggtgacca ctaaaggctt tcgcgatcag gctatccgca ctggtggccc
      481 tggcgtaggc ggcgatgccg atggcaagcg cgaagaacgg cagcatttcc cacagcgcct
      541 cgagggtaaa caacaggctg agcggcagct gctgcaggtc gagtaacgcc agcaggattg
      601 acatcgttgc catggccatg aaaacccggt ccctgcagac ggagccgagt aactgcagta
      661 agggctggtt gcggtttcgg gtttcggtta gtgtgtccat ttttctagta atctcgaaat
      721 agtaagcctg aaaaatcagg ccgcggtttc actggagtcg atgtcggcgc agcattctgc
      781 ggtcaggtaa tcgaccaggc cctggattac gccgaactcg actctgcaga tcaaagtcgc
      841 ctgccggcgc tgttggcgga taacgccggc ggatttcaaa tgggttatgt gatgggttag
      901 ggtcgagccc gggatcccca gtttcttctg gatcacgccg accggcaggc cctcgtctcc
      961 ggtgcgcacc aacagccgca cgatcctgag ccgggtgggg ttgccgatct tggcgagaat
     1021 attggcggcg gtatccagtt tcatgggggt gatactagtc gatgcgtctc cattcgtcaa
     1081 ctatatttct agaattatcg aaataatgta actgcttgcg agtgatatcg ccgtccgacc
     1141 acgcaggcgt cgatagtatg gcctagcgag atatatgatc gagaaagcgg gtgaacagcc
     1201 ccgggttgtt ttgcatggtc ggcagcgcga gcacctcgtc gcaaacccgg attgcggccg
     1261 cctggtcgag gccgccgtat tgcagcagtc ccgtcgcctt ggcgcgcatc tcggatctat
     1321 ccagcgctaa ctcggggtct cccttgcaat ccttgcgtga aaccttgaaa gcctcgccgg
     1381 attcggtcac cgcggtgacc tcggcgcccc aggataccgg gtagttcgac gcgtagggat
     1441 cgacgataga tacgctggtg gcacggcgca gccgtgcgct gcgctcgcgc gcgtcggcgt
     1501 cgaacgagtc cagcccgacc acgccatcgg tcaacgcaat tgcagccgtg tgataaagcg
     1561 aaaacttggc ctggtactcg gtctcgggct cgcggcaatc gcaaacgtcg agcgccgcct
     1621 ggtaggtctt gatgccgacc tccctggtcg gcttgccgtc gagcaactga tggatttcga
     1681 gcgcgcagtc gatgattgga tgcgtatgcc gacaggaagg ccagggtttg atcgaagtca
     1741 gcgagagttg ccacttgtcg tccggctggc gcagtatgtt ttccggtatc ggatcgtcgc
     1801 aggtgccggc gaacaggccc ttgctgccct cgagaatttc ggccgggcca gtaaagccat
     1861 gcgcggccag ttcggcggcc agcaggcccg attcgctggc gcggcccgca tgcaggtgtt
     1921 tgctcatcgc gccggtctcc atgaattgcc acaggccgct ggactgggtg ccggcgttgc
     1981 cgagcgcatt caaggtctgt tcgtcggtca attcgagcag gctggccgcc gccatcgccg
     2041 agccgaaggg tccacaggtc gcggtgttgt gccagatacg ataatgcgcc ggtcccacgg
     2101 cagcgccgat acgacacatg gcttcgaaac cgtgcagtat tgcgcgcagc atttgttcac
     2161 cgctggcgcc ggttttttcg ccgagggcga gtaccgccgg caccacgacg catcccgggt
     2221 gagttaccga ggcgcgatgc agatcgtcgg tctcggtaat gtgcgtcagt gcgcccatca
     2281 gcagcgcctt gcgtttgagg tcgagatcac cgtcagtagc ccagccacgt aaaatcgcgc
     2341 caaccggtgt cgagctgccg gcataagcgg tggccacggc gtccagcgtg aacagcgccg
     2401 cgtcgtcgag gtcggtcgtt gaaaccggcc gcttgcggat caggccgatc aagtctagtg
     2461 tcaggctcat cgaagttcca atatagtaat ccgccagggg attcgaatgc cccggaatat
     2521 aacgcaacat gtatcgggag gacagactat gtcggcaaag caggtaatcg gtgggccgct
     2581 ggaaattgat ggccgggtgc tttcgctgtc gaaagcggtt cgcgccgggg atttcgtttt
     2641 cgtaaccgga caggtgccga tgcgcgacgg caaaccaata accgatggca gtatcgagga
     2701 tcagacccgg gcctgcctgg atggcctgag cgaaattctg gccgaggcgg gctgcgaact
     2761 tggagacgtc gtcaaatcga tggtctggct gaaaaagcgc gaagactttc ccgggttcaa
     2821 ctcggtctac
//
LOCUS       DATCZV010000351         3842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_81108, whole genome shotgun sequence.
ACCESSION   DATCZV010000351 DATCZV010000000
VERSION     DATCZV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81108"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VIW27_07885"
     CDS             complement(<1..597)
                     /locus_tag="VIW27_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5739631.1"
                     /translation="MKAMILAAGRGERMRPLTDRVPKPLLPVAGKPLIVHHLEALSRA
                     GFAEVVVNLSWLGAQIRELLADGGDFGLKIDYSEEAEALETAGGIRQALPLLGEQFVV
                     VNADIFTDYDFSRLRGIEAAAHLVLVPNPTHNDGGDFALAETLVLNRGEPRFTFSGIG
                     CYRRELFAALKPGKRPLAPLLREAADAGRVSGELFTGNW"
     gene            complement(594..1601)
                     /locus_tag="VIW27_07890"
     CDS             complement(594..1601)
                     /locus_tag="VIW27_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5739632.1"
                     /translation="MNARSSQLNQWLEQLGFRDYRLSPASEDASFRQYLRLEADGHSQ
                     IVMDAPPELEPCEQFVKVAKLLRGAGLSAPQINAQNLEQGFLLLTDFGGCDYLSQLHD
                     DTEAELYRDALAALLLMQSRIDAGELPPYDEALLFREMDLFHDWFLGSMLGLELNAQQ
                     QRAWQSCRRRLADNALEQPQVFVHRDYHSRNLMRIVRRNPGILDFQDAVRGPITYDLV
                     SLLRDCYIAWPAARVEELALDYYQAARANGLLKADSVQFLRWFDLMGMQRHLKAVGIF
                     ARLKIRDGKSRYLQDIPRTLEYLRAVGAAHQSMAGLAALIDELELASRAAALAKTVSP
                     Q"
     gene            1676..2458
                     /gene="mazG"
                     /locus_tag="VIW27_07895"
     CDS             1676..2458
                     /gene="mazG"
                     /locus_tag="VIW27_07895"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY5739633.1"
                     /translation="MRPIDKLLAVMQDLRHPEQGCPWDLRQTSASIAPHTLDETHEVL
                     DAIERGDTENLKEELGDLLFNIVFHARIAEEQGQFDFDDVARGITDKMLRRHPHVFGD
                     EHEREISDQQLSRQWQSLKQAEKKHQQPGMPEIDEAAGSTMFRARELQKQAAKFGFDW
                     PDIRPVFDKLDEELRELRQAYEAGDAAAISDEVGDLLFVCINIARHAGVNAEMSLRGT
                     NRKFQRRFAYVRERMAEAGVEMEQGQLELMEEFWQQAKGQVG"
     gene            2466..3053
                     /locus_tag="VIW27_07900"
     CDS             2466..3053
                     /locus_tag="VIW27_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5739634.1"
                     /translation="MKRGRLDTEQRLLDAVGGLLFDEGYPAVGINAIARRAGCDKVLI
                     YRYFGGFDELLLAFAETTELWWQVDEIISETAEDCAAIALPDYLQTLLDRYLAALESR
                     PLTLEIMAWEMSAQNNLIAALARVRSERGMELVKRIRAYYRQPNIDIGGILGVFGAAI
                     NYLVIRTRKHSRQYKTEEWWRLQQTIGKLLQAYAD"
     gene            3043..3534
                     /locus_tag="VIW27_07905"
     CDS             3043..3534
                     /locus_tag="VIW27_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739635.1"
                     /translation="MPTDVLRLAEVETAEVADLLARFGLHLQTCASGHSITASYWGEP
                     EAGIAGDRLYARGDTPLHSILHEASHYICMDAGRRQRLHRDAGSDEAEENAVCYLQIL
                     LADAFESFGRERMLADMDRWGYSFRLGSSRAWFEHDATDALEWLQHHGLVDHRQRPGY
                     RLR"
     gene            complement(3654..>3842)
                     /locus_tag="VIW27_07910"
     CDS             complement(3654..>3842)
                     /locus_tag="VIW27_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5739636.1"
                     /translation="NQGIVLGWHSQVAGMIERGELVRVGAFEIAAPGAFYLTWDRQRP
                     LSDAAQRLRDWLLETCRP"
BASE COUNT          775 a         1221 c         1195 g          651 t
ORIGIN      
        1 ccagttgccg gtgaataatt ccccgctcac tcgcccggca tcggcggcct cgcgtaatag
       61 cggcgccagc ggccgcttgc cgggtttcag cgcggcaaac aattcgcgtc gataacagcc
      121 gataccgctg aaggtgaaac gcggctcacc gcgatttaga accagcgttt ccgccagcgc
      181 gaaatcgccg ccgtcgttat gcgtcgggtt aggtaccagt accaggtgtg ccgcggcttc
      241 gataccgcgc aggcgggaaa aatcgtaatc ggtgaatatg tcggcgttga cgacgacgaa
      301 ctgctcaccg agcaatggca acgcctggcg aatgccaccc gcggtttcca gcgcctcggc
      361 ctcctcgctg taatcgattt tcaggccgaa atcgccgcca tcggcgagca gctcgcgaat
      421 ctgcgcgccc agccagctga ggttgacgac gacctctgcg aaaccggccc gcgacagggc
      481 ttcgagatga tgcacgatca atggcttgcc ggccaccggc agcaacggct tgggcacgcg
      541 gtcggtcagc ggacgcatgc gctcgccgcg gccggccgcg agaatcatcg ccttcattgc
      601 gggctcaccg ttttcgccag cgccgcggcg cgcgacgcca gctccagctc gtcgatcagc
      661 gccgcgaggc cggccatcga ctggtgcgcg gcaccgactg cacgcaggta ttcgagggtg
      721 cgcggtatat cctgcaggta gcgggacttg ccgtcgcgaa tcttcaagcg cgcaaaaatg
      781 ccgacggcct tcaggtggcg ctgcatgccc atcagatcga accaacgcag aaattgcaca
      841 ctatcggcct tcagcaagcc gttggcgcgc gccgcctggt aataatccag cgccagttcc
      901 tcgacacgcg ccgccggcca ggcgatgtaa caatcgcgca gtaacgatac gagatcgtag
      961 gtgatcgggc cgcggacggc atcctggaaa tcgagaatcc cgggattgcg gcgcacgatc
     1021 cgcatcaggt tgcgtgagtg gtaatcgcgg tgcacgaaaa cctgtggttg ctcgagcgca
     1081 ttgtcggcta gccggcggcg gcaggattgc caggcccgtt gctgttgcgc gttcagctcg
     1141 agaccgagca tgctgcccag gaaccagtcg tgaaacaggt ccatctcgcg aaacagcagc
     1201 gcctcgtcgt agggcggcag ctcaccggca tcgatgcggg actgcatcag cagcaacgcc
     1261 gcgagcgcat cccggtagag ttcggcctcg gtgtcgtcat gcagctgcga caggtaatcg
     1321 caaccgccga aatcggtcag caacaggaag ccctgctcga ggttttgcgc attgatctgc
     1381 ggcgcgctga ggccggcacc gcgcagcagc ttcgcgacct tgacgaattg ctcgcagggt
     1441 tccagctcgg gcggagcgtc catcacgatc tgcgagtgcc cgtcagcctc gaggcgcagg
     1501 tactggcgaa aactggcatc ctcggaagcc gggctgaggc gataatcgcg aaaaccgagc
     1561 tgctccagcc actggttcaa ctgtgacgat ctggcattca ttttctgctg gcggctgcga
     1621 tcctgttaaa aggccggtat tatcggggtt ttgaaacggc aaagccatag agcccatgcg
     1681 ccccatcgac aaactgctgg cagtcatgca ggacctgcgc catcccgagc agggatgccc
     1741 ctgggatctg cgccagacca gcgccagcat cgcaccgcac accctcgacg aaacccacga
     1801 ggtgctagac gcgatcgagc gcggcgacac cgaaaacctc aaggaggaac tcggcgacct
     1861 gctgttcaat atcgtatttc atgcgcgcat cgccgaggag cagggacagt tcgatttcga
     1921 cgacgtcgcg cgcggtatta ccgacaagat gctgcgtcgt catccgcatg tattcggcga
     1981 tgagcacgaa cgcgagatca gcgatcagca actgtctcgc cagtggcaat ccctcaagca
     2041 ggcggagaag aaacatcagc agccgggcat gcccgagatc gacgaggccg ccggttcgac
     2101 gatgtttcgc gcccgcgaac tgcagaaaca ggctgcgaag ttcggcttcg actggccaga
     2161 catcaggccg gttttcgaca agctcgacga agagctgcgc gagttacggc aagcttatga
     2221 ggccggcgat gccgccgcga ttagcgatga agtcggcgat ctgttattcg tctgcataaa
     2281 tatcgcccgc cacgcaggcg tcaacgcgga gatgtcgctg cgcggtacta atcgcaagtt
     2341 ccagcggcgc ttcgcctacg tccgcgagcg catggccgaa gccggcgtcg agatggaaca
     2401 ggggcagctc gaactgatgg aagaattctg gcagcaagcc aagggccagg tcggatgaac
     2461 aactcatgaa acgaggtcgt ctcgataccg agcaacgcct gctcgacgcg gtcggcggcc
     2521 tgctgttcga cgaaggttac ccggcggtcg gcatcaacgc catcgcgcgc cgcgccggtt
     2581 gcgacaaggt actgatctac cgctacttcg gcggcttcga cgagttgctg ctggctttcg
     2641 ccgagaccac cgaactgtgg tggcaagtgg acgaaatcat ctccgagacc gccgaggact
     2701 gcgctgcgat cgcgctgccc gactatctgc aaaccctgct cgaccgctac cttgcggcgc
     2761 tcgaatcgcg cccgctgaca ctcgagatca tggcctggga aatgtcggcg caaaataacc
     2821 tgatcgcggc gctggcgcgc gtccgcagcg agcgcggcat ggagctggtg aaacggattc
     2881 gcgcctacta ccggcagccg aatatcgata tcggcggtat cctcggcgtt ttcggcgcgg
     2941 cgatcaatta cctggtgatt cgcacccgca agcattcgcg ccagtacaag accgaggaat
     3001 ggtggcgcct gcagcaaacc atcggcaagc tgttgcaggc ctatgccgac tgacgtgttg
     3061 cggcttgcgg aggtcgaaac agccgaagtc gccgacctgc tcgcccgctt cggtctgcac
     3121 ctgcaaacct gcgctagcgg tcattcgatt acggcgagtt actggggtga acccgaagcc
     3181 ggcatcgccg gcgatcgcct ctacgcgcgc ggcgatacgc cgctgcactc gatcctgcat
     3241 gaagcgagcc attatatttg catggacgcg ggacgccgtc agcgcctgca ccgcgacgcg
     3301 ggcagcgacg aagccgagga gaacgcggtc tgctacctgc agatcttgct cgccgacgca
     3361 ttcgaatcct tcgggcgcga gcgcatgctg gccgacatgg accgctgggg ctacagtttc
     3421 cgcctcggct cgagccgcgc ctggttcgag cacgacgcca ccgacgccct cgaatggctg
     3481 caacaccacg gcctcgtcga ccaccgccag cgccccggct accgcctccg ttgatccagg
     3541 cccgaaccgc cgggggccag acagcaggga ggattttggt cagaacaaca attcagcaat
     3601 ccaccccgcc ggcggagttc ttgtactgac caaaaactcg agtggaaaca atctcagggg
     3661 cggcaggttt cgaggagcca gtcgcgcagg cgctgcgcgg catcggacag cggtcgctgg
     3721 cggtcccagg tgagatagaa tgcgccagga gcggcaattt cgaatgcgcc gacccgcacc
     3781 agttcgccac gctcgatcat accggcgacc tgcgagtgcc agccgagcac gataccctgg
     3841 tt
//
LOCUS       DATCZV010000352         4882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_240805, whole genome shotgun sequence.
ACCESSION   DATCZV010000352 DATCZV010000000
VERSION     DATCZV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240805"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1887
                     /gene="ilvD"
                     /locus_tag="VIW27_07915"
     CDS             163..1887
                     /gene="ilvD"
                     /locus_tag="VIW27_07915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY5739637.1"
                     /translation="MATKKSASKKSASKKPGIRQFSSLVVDGVERAPSRSMLRAVGFD
                     DADFKKPQVGIASTWSMVTPCNMHINQLADKAAEGAAMANGKPVIFNTITISDGISMG
                     TEGMKYSLVSREVIADSIEAVTGCQGFDALVTIGGCDKNMPGCLIGMARLNRPSVFVY
                     GGTIMPGCHKNKPTDVVSVFEAVGAQAQNKISARELKQIESTAIPGPGSCGGMYTANT
                     MASAIEALGMSLPNSSAQAAISTDKTQDCIEAGRAAMYLLRNNIRPSDIMTKKAFENA
                     ITVVIALGGSTNAVLHLLAMASAIGVRLKLDDFTRIGKRVPVLADLRPSGRAMMSELI
                     EIGGIQPLMKMLLDAGLLHGDCLTVTGKTLKQNLARVKPYPDSQDIVHALNDPIKKDS
                     HLVILYGNLAPEGSVAKISGKEGLSFTGRARIFASEEQALKKILDGTVKKGDVIVVRY
                     EGPRGGPGMREMLSPTSAIMGKGLGKDVAFLTDGRFSGGSHGFVVGHVTPEAAVGGPI
                     ALLKNGDQITIDAEKREVNVKLSAAELKKRAAAWKAPKPRYKRGVLAKYARLVSSASL
                     GAVTDLPD"
     gene            2178..3326
                     /gene="argE"
                     /locus_tag="VIW27_07920"
     CDS             2178..3326
                     /gene="argE"
                     /locus_tag="VIW27_07920"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY5739638.1"
                     /translation="MKQSLPETREMIAQLIAAPSVSSAQPEHDMGNRAVIDLVAEWAA
                     NLGFDIEIQPVSAGKFNLVARAGDGSDGLVLSGHSDTVPCNPELWTSDPFAAREHEDR
                     IYGLGSCDMKSFLALALTACSSFELHKLARPLTLVATADEETTMNGAKLIADNGRKLG
                     RYCLIGEPTSLQPVRQHKGCLTLSVTYTGLSGHASDPARGNSALDGMHQFMGRLYDYR
                     DRLQQRFRDPAFEIPTPTMNLGHIHGGDSSNRICGNCTLLIDIRYLPSMSLAALQADI
                     GQLAAEVAEARGLEQQTCMIGNGLSPMNTDEASEIAQYLTRMTGSKAATAAFGTEAPY
                     FNSMQTETVVMGPGSIEQAHRPDEFLAVAQIGPTVSLLENLIDRFCNS"
     gene            3323..4465
                     /locus_tag="VIW27_07925"
     CDS             3323..4465
                     /locus_tag="VIW27_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HEY5739639.1"
                     /translation="MKIVADRAIPQIEEAFADCGELELLPGREIARHHLADCQCLIVR
                     TVTGVGRELLEGSPVEFVGSATIGTDHVDLEYLRAAGIGFSNAAGCNAEAVSEYVVSG
                     LFALAESRGFDALGRRAGIVGFGNVGRRLKHKFDALGIDTLLCDPPLAAAGATAESFE
                     TLDTLLAECDLLSLHTPLTRQGEHATFHLLDARRLQALVPGCLLVNTARGEVVDNRAL
                     LDLLGRREDLVTFIDTWEDEPRVLRELLRRVDLATPHIAGYSVEGRLRGTQAVLDAAA
                     AHFGWTPGWHMSRHLPPPTLLQPSATANPRDFWRQLFAQHLDIWRDDKAFRVNAEFAD
                     EERAKHFDSLRRVYADRLEYARFTVTAASAGRQAGVLRELGFEITN"
     gene            complement(4535..>4882)
                     /locus_tag="VIW27_07930"
     CDS             complement(4535..>4882)
                     /locus_tag="VIW27_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5739640.1"
                     /translation="IDDFGTGYSSLSYLKTLPISSIKIDRGFVRDLCTDENDQKIVKT
                     LIKMAHSMDMKVVAEGVEQEDQMKLLKRLLVDQVQGHLISEPAPPIEVESMIAGNKDA
                     PGEQSNIVPLRGK"
BASE COUNT          953 a         1565 c         1501 g          863 t
ORIGIN      
        1 acaatcgtat cggtcatgtg cgcttcggct cagatccgca ttgcatcaag tataggcaag
       61 aaatcgccga tttgtgggct cgccggggat ttattcactt gcaacggcta acgcgtttgc
      121 ctaacctacg gcgtcttcag ccagcaaaaa acgaccctgc caatggccac caaaaaatcc
      181 gcttccaaaa aatccgcttc caaaaaaccc ggtatccgcc agttctcctc gttggtcgtc
      241 gacggcgtcg aacgtgcgcc gagccgctcg atgctgcgcg cggtcggatt cgacgatgct
      301 gatttcaaga aaccccaggt agggatcgcg tcgacctgga gcatggtcac gccctgcaac
      361 atgcatatca accagctcgc cgacaaggct gccgagggcg cggcgatggc caatggcaag
      421 ccggtaatct tcaacaccat caccatttcc gacggcatct cgatgggtac cgagggaatg
      481 aaatattcgc tggtgtcgcg tgaggtgatc gccgattcca tcgaggccgt caccggctgc
      541 cagggattcg atgcactggt tacgatcggc ggctgcgaca agaatatgcc gggttgcctg
      601 attggcatgg cgcggctcaa ccggccttcg gtgttcgtct atggcggcac catcatgccg
      661 ggctgccaca agaacaagcc aaccgacgtg gtatcggtct tcgaggcggt cggcgcgcag
      721 gcccagaaca agatcagcgc ccgcgaactc aagcagatcg aatcgaccgc gattccgggt
      781 cccggctcct gcggcggcat gtacaccgcc aataccatgg cttcggcgat cgaggcgttg
      841 ggcatgagcc tgccgaacag ctcggcacaa gcggcaattt ccaccgacaa gacacaggat
      901 tgcatcgagg cgggccgcgc ggcgatgtac ctgctgcgca acaacattcg gcccagcgac
      961 atcatgacca aaaaggcttt cgaaaacgcg atcaccgtgg tcatcgcgct cggcggctcg
     1021 accaacgcgg tgctgcacct gctggcgatg gcgtcggcga tcggcgtcag actcaaactc
     1081 gatgatttca cgcgcatcgg caagcgcgtg ccggtactcg ccgatctcag gcccagcggc
     1141 cgcgcgatga tgtcggaact gatcgagatc ggcggcattc agccactgat gaaaatgctg
     1201 ctcgacgcgg gcctgctgca cggcgactgc ctgacggtga ccggcaagac gctgaagcag
     1261 aacctggccc gggtcaagcc ctaccccgat agccaggaca tcgtgcatgc gctcaacgac
     1321 ccgatcaaga aagacagcca cctggtcatt ctctacggca atctggcgcc ggagggctcg
     1381 gtggcgaaaa tctccggcaa ggaaggcttg agcttcaccg gcagggcgcg catcttcgct
     1441 tccgaggagc aggcgctgaa gaagattctc gacggcacgg tcaagaaggg cgacgtgatc
     1501 gtggtgcgct acgagggacc caggggtggc cccggcatgc gcgaaatgct gtcaccgacc
     1561 tcggcgatca tgggcaaggg gctcggcaag gacgtggcct ttctgaccga tggcagattt
     1621 tcgggggggt cgcatggttt cgtggtcggc cacgtcacgc cggaagcggc ggtcggcggc
     1681 ccgatcgcgc tgctcaagaa cggcgaccaa atcaccatcg atgccgagaa gcgcgaggtc
     1741 aacgtcaagc tcagcgccgc cgaactgaaa aagcgcgccg ccgcgtggaa agcacccaaa
     1801 ccgcgctaca agcgcggcgt gctcgccaag tacgcgcgcc tggtcagctc tgcctcgctg
     1861 ggtgcggtta cggatttacc ggactagact gaaaattgcc ccagccgctg tcgagcccga
     1921 tatctagccg ctggcgagga gtgaggttcg tatgcagcca acatatccct cgaagaaaag
     1981 catattcgga cggctcgagg cctggttcgg cactgacaaa tcctgttccg cctggacttg
     2041 tcgagacgca cagctgtacc tcgagcccta gccatggcaa gggctctcga tccagcccgg
     2101 cgtccagacg catctgtcgt tcgtcggcgc ccgaaatacc ggcacaattc tcggtctcgc
     2161 ccggcacctg gtactagatg aagcagagtc ttccagagac gcgcgaaatg atcgcgcaac
     2221 tgatcgcggc gccctcggtg agcagcgcgc agccggagca cgacatgggc aaccgggcgg
     2281 tcatcgacct ggtcgccgaa tgggctgcaa acctcggctt cgatatcgaa attcagccgg
     2341 tcagcgcggg caagttcaac ctcgtggcgc gagccggcga cggcagcgat ggcctggtgc
     2401 tgtcgggaca cagcgacacc gtgccctgca acccggagtt atggaccagc gacccgttcg
     2461 ccgcacgcga gcacgaagac cgcatctacg ggctcggcag ttgcgacatg aagagtttcc
     2521 tcgcgctggc gctcacggcc tgcagcagtt tcgagctgca caaactcgcc aggccactga
     2581 ccctggtcgc caccgccgac gaggaaacca cgatgaatgg cgccaagctg atcgccgaca
     2641 acggccgcaa gctcggccgc tattgcctga tcggcgaacc taccagcctg cagccggtgc
     2701 gccagcacaa gggctgcctg acgctgtcgg taacctacac cggcctttcc gggcacgcca
     2761 gcgaccccgc gcgcggcaac agcgcgctcg acggcatgca ccagttcatg ggccggctgt
     2821 acgactaccg cgacaggctg cagcaacgtt ttcgcgatcc ggctttcgag atcccaacgc
     2881 cgaccatgaa tctcggccat attcacggcg gggacagctc caaccgtatt tgcggcaatt
     2941 gcacgctgct aatcgacatt cgctacctgc cgtcgatgtc gctggccgcg ttgcaggcag
     3001 atatcgggca actggccgcc gaggtcgccg aggctcgcgg gctcgagcag cagacctgca
     3061 tgatcggtaa cgggctgtcg ccgatgaata ccgacgaagc cagcgagatc gctcaatacc
     3121 tgacccgaat gaccggtagc aaagccgcca ccgccgcctt cggcaccgag gcgccctact
     3181 tcaattcgat gcaaaccgag actgtggtca tgggccctgg cagcatcgag caggcgcatc
     3241 gaccggacga gttcctcgcg gttgcgcaga tcgggccgac cgtcagcttg ctggaaaacc
     3301 tgatcgatcg cttctgcaat tcgtgaaaat cgtcgccgat cgcgccattc cgcaaatcga
     3361 ggaggccttt gccgattgcg gcgaactcga attgctgccg ggccgcgaga tcgcccgcca
     3421 ccacctcgcc gactgtcagt gcctgatcgt gcgcaccgtg actggcgtcg gccgcgaact
     3481 gctcgagggt tcgccggtcg agtttgtcgg cagtgcgacc atcggtaccg atcatgtcga
     3541 cctcgaatac ctgcgggccg ccggtatcgg tttcagcaac gcggccggct gcaacgccga
     3601 ggcggtctcg gaatatgtcg tcagcggcct gttcgcgctg gccgaaagcc gtggattcga
     3661 tgcgctgggc cggcgcgccg gcatcgtcgg tttcggcaac gtcggccgtc gcctgaagca
     3721 caagttcgac gcgctcggca tcgacaccct gctgtgcgac ccgccactgg ctgcagccgg
     3781 cgccactgcg gaatcgttcg aaactctaga taccctgctt gccgagtgcg acctgctcag
     3841 cctgcatacc ccgctgaccc ggcagggcga gcatgcgacc ttccacctgc tcgacgcccg
     3901 tcggctgcag gcgctggtac ccggctgcct gctggtcaat accgcgcgcg gcgaagtcgt
     3961 cgacaatcgc gcgctgctcg acctgctcgg ccgccgcgag gacctggtca catttatcga
     4021 cacctgggaa gacgagccgc gggtgctgcg cgaactgttg cgacgcgtcg acctggcgac
     4081 tccgcatatc gcggggtaca gcgtcgaggg ccgcttgcgc ggcacccagg cggtgctgga
     4141 tgcggcggcc gcgcatttcg gctggacgcc gggctggcac atgtcgcgcc atttgccgcc
     4201 gccgacgctg ctgcagccat ccgccaccgc gaacccgcgg gacttctggc ggcagctgtt
     4261 tgctcagcat ctggatatct ggcgagacga taaggctttt cgtgtcaacg cggagttcgc
     4321 agacgaggag cgggcgaaac atttcgacag cctgcgccgg gtttacgccg accgcctcga
     4381 gtatgcgcgc tttacggtga ccgcagccag cgccggccgg caggccggcg tgctgcggga
     4441 actgggattc gagatcacga attaacgtcc gggaactatg cgatcgtggc ctgctgcagc
     4501 cggccacggc tagcatcaga gctgtcgggg tggattactt gccgcgcaac ggcacgatat
     4561 tggactgttc tccgggcgcg tccttgttgc cggcaatcat cgattcgacc tcgatcggcg
     4621 gggcgggctc gctgatcagg tgaccctgca cctggtcgac cagcagtcgc ttcaataatt
     4681 tcatctgatc ctcttgctcg acgccctcgg cgaccacctt catatccatc gaatgagcca
     4741 tcttgatcag agtcttgacg attttttgat cgttttcgtc ggtgcagaga tcgcgcacga
     4801 agccacgatc gatcttgata ctcgaaatcg gcagcgtctt caggtaactc agtgacgagt
     4861 agccggtccc gaagtcgtcg at
//
LOCUS       DATCZV010000353         9356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_188143, whole genome shotgun sequence.
ACCESSION   DATCZV010000353 DATCZV010000000
VERSION     DATCZV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188143"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VIW27_07935"
     CDS             <1..1189
                     /locus_tag="VIW27_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEY5739641.1"
                     /translation="DRPFARIYRIDYLNMARTTNTSIDVATQIASTGTVDSGGGGSGG
                     GSNSSASVQNQSVNDFWVSIESNITQLAAADGVDASTIVNRESGTISVRTTSAAHESI
                     QNFIDNVVNSARRQVLIEATVVEVTLNDDFQAGVDWSRIAVGDGWSLSQNVLAETIGA
                     ATSPVISATYANSNDERDISGAIKALDAFGEVSVMSSPKIMALNNQTSILKVVDNVVY
                     FTQEIDREPATDNSPEVITIDTTVNTVPVGFVMNVTPFITAEDEIILNIRPTISRVLG
                     FVAPPSIVIPGTTELLEQSAVPQIQVREMESVLRVQSGNTAVIGGLMQDTADELNAGV
                     PGLHDAQGFGLLFGTQSRQYDKTELVIFLRPRVIDNASLDTNLRDFSKYLKPRVFTPE
                     QSD"
     gene            1190..3583
                     /locus_tag="VIW27_07940"
     CDS             1190..3583
                     /locus_tag="VIW27_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5739642.1"
                     /translation="MSLLLDALKKAAEQKAAKSRQDESAAARASNAGPDVPGLEPAGG
                     DAEPGPPSRYRPGDEAEHDDSAFDPRVEPADAGDQVTTEGREDETLIFSVDDAAEIVD
                     DDSPIAAPADDDTDLKPLDGVAGGADGIDVDEIEVSRLDGDETDLSQFLAEQSAADQG
                     ETGSADAPRLDTDDTDLGLLAAADEAAVEAADNAFDSGLAEGGDTDFELLEVPDDEDL
                     ELQPLGDDELDFDLLPLAADADEASDLDEQGFAAGDSGAGAGDAGADDEDMSLLLIER
                     EPTQITSPRSTTAPWSPQDALQSLQAEAPTAADLGLIEAARERPDKQPALSEDGTQTR
                     NRTSNTATTQSRSSDTATTGIATATANLGKVPRPDPNAAQNHAPDNYDRTLMRLPNEE
                     AARLFADITSGSEVVMTPDYAKRVFRSKSSASRIQHLKMYVGISIAILLAIAVYGIFE
                     YQNESENIEAELRPLKNDPMPGMLRTETANTEPETMFADAEVNQRALEILQNAEINAE
                     LGNAGVATAAEGNDDSEIAADETAAEMAMEPLPSPAPTHSAESPAAVEDTATSQSIIL
                     SEVSRQAPAERATVASTATSGSQTRAATSGDSRLQIETGERLEEKQVLLREAYGAFRA
                     GDYARALKGYNRVLEIDPANRNALLARGAINVHNGNGVAAINDYQALLLANPKDSLAM
                     ASLLAIANVSPLEIETQLKLMIRDEPDSPYLNFALANVYAAQDRWQEAQRHYFTALQN
                     NPDDPNYAYNLAVSLEHISQPRAAVSYYRRALENYNKGLATFSRDAVGRRLEKLANS"
     gene            3580..5286
                     /locus_tag="VIW27_07945"
     CDS             3580..5286
                     /locus_tag="VIW27_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5739643.1"
                     /translation="MNAENKPKRLGEILMDQGVITEDQLNIALTEQKKVHEPLGKLLV
                     SLGFVTEAIMRAALGEVLEQESVDLSRVVPDPDAIKMINSDTARKYKIIPLNYDPIRG
                     RLTIAMSDTFNLFTLDRIRIQIGQHIELVPVLAGEAEISNAIDQFYGYELSIDGILRE
                     IETGEADFTNYQGDIEEYSQPLVRLVDVMLSDAVKRGASDLHFEPEENFLRIRYRIDG
                     VLRQIRSLHKTYWSAIAVRLKVMADMNIAETRAPQDGRISRTIVGRQIDFRVASQPTI
                     HGENIVLRVLDREKGVIPLESLELPDESLRTLKIMMSRPEGMILVTGPTGSGKTTTLY
                     SLLNYLSSEQINIMTLEDPVEYPFPLIRQTSVNEAAGVGFASGIRSLMRQDPDVILVG
                     EIRDEDTAEMAFRAAMTGHQVYSTLHTNSAIGALPRLRDIGIIPEIMAGNIIGVVAQR
                     LIRKLCKYCKTAYAPSKEELRLLKNDRDIVPKKIYRAKGCTSCDNTGYKGRMALLEIL
                     KMNSDLDDLLAHGATTKEIKDAAYKQGFFTLADDGVRHILNGVTSIKEVARVADLTER
                     MG"
     gene            5289..6494
                     /locus_tag="VIW27_07950"
     CDS             5289..6494
                     /locus_tag="VIW27_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5739644.1"
                     /translation="MPLYQYKAIDASGKFISGSLDAGNINDLELRLEKMEMDLVTYKQ
                     KEPGSDLFGRNKISRRDLINFSFYLEQLTRAGVPILEGLTDLRDGEENPTFRDVITGV
                     IEAIEGGNSFSQALGLYPKIFDEVFVSLIRVGERSGKMNEVLIDITETLKWQDELLAK
                     ARKIMMYPTVIGILVLSVVVFMMIFIVPDIMDAIVALGGEIPLETRMLMATSDFLVQF
                     WYLVVAFPFLVFFVIRYFYNSNSRVRFKVDGMALKVFILGPVNEKIKISRFSRYFSLM
                     FASGITVLDAINLSKAVVSNSVLEDGIERAWQQISEGSSISEAFKNIGIFPPLVVRML
                     RVGESSGQMDKALNNVSYFFDRDINDSIDKMEPLLQTTLMLVIGTVVMWLALAVLGPI
                     YETISTIEF"
     gene            6505..8106
                     /locus_tag="VIW27_07955"
     CDS             6505..8106
                     /locus_tag="VIW27_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739645.1"
                     /translation="MKRVLYFTGYRMVAQEWLGRKLVSSVYFEPDEQGLDLFSAYLQS
                     FSNEPVRMLVDLIEEEFRQIRVPLLRGSDRQAILKRNYAKYFRHSDYKFAISQAVEKK
                     TRKEEKLLLIGLTNQYLLEPWLKIIEQSQTPLSGIISLPLLSEDYVKELKSAHRAIIL
                     VSQQVPSNLRQSVFVDGRLVLSRLVPIASFYQGDYASDLLRDIESTQRYLINQRIVER
                     AETMAVHILTNKRHLERLQSRCQQETQYDFTIHEINELLAREKIDVSDEQDFSSGLFC
                     YLASRKITLNHYAQPRERRFFQYHRAGLALRALSIALIAVGAGFALNGGVANLLYKQT
                     IEETRLIEQKYRSKYNQLSQNRVDSNTSTSSMQYIVQTVDQIENRYLRDPEELLAMIS
                     EDISIFPDIRVRRLDWFVSNSSAAVDAGDVSWGRSGQTGNRSSSRDRNKPAAKTGYFE
                     IVRLEGEFLNFDGNFRYALSAVDDLEKAMTESGKYYSVEITKRPLDIESDNRLSGDAG
                     VFGGSQRSLRAELALRVVREVPQVE"
     gene            8099..8695
                     /locus_tag="VIW27_07960"
     CDS             8099..8695
                     /locus_tag="VIW27_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739646.1"
                     /translation="MNKVVWKSLRWPLIIFVIALVIASAFGYFGMHLEDSAQQDYQRA
                     VSSLRSTHQRYRELVNDIDLLEQYRNRYTEYKDSGLVGGERRLSWIESLEATNGVLRL
                     PTLTYNLHPQEGFERPGFSVQQGVMVQSSPMDLSMDLLHEEDLFALLEGLRLSISNLF
                     TVDSCSVNRVSGIGSSLETRDTNLQGRCMIRWVTIDVG"
     gene            8685..9140
                     /locus_tag="VIW27_07965"
     CDS             8685..9140
                     /locus_tag="VIW27_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739647.1"
                     /translation="MSVKRLVLVLLLSYGIQLPALAAELATLFTTPQERRLIDRNRYK
                     QAEAEPQPQPAADPGDVFAVDDVEYTLEEVTREYRVSGITLSREGSHTVWINAKAYED
                     GARLDDDSRIQVIAGDQIRVRITAPDGRQYLAASGETVEISLLVPVESE"
     gene            9137..>9356
                     /locus_tag="VIW27_07970"
     CDS             9137..>9356
                     /locus_tag="VIW27_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739648.1"
                     /translation="MMRPGYSQSGYALLLVLLVLMGAGGVVLAGFTQQVKHEAERQRY
                     EHNQRVLREAKQALLVYAYRYPDIALAFN"
BASE COUNT         2001 a         2810 c         2812 g         1733 t
ORIGIN      
        1 ggaccggcca ttcgcgcgta tctaccgcat cgattacctc aacatggcac gcactaccaa
       61 tacctcgatc gacgtggcga cgcagatcgc ctccaccggt accgtcgaca gcggcggcgg
      121 gggttcgggt ggcggcagta attcgtcggc ctccgtgcaa aaccagtcgg tcaacgactt
      181 ctgggtcagc atcgagtcca atatcaccca gctggccgcg gccgatggcg tcgatgcaag
      241 taccatcgtc aaccgggaat cggggaccat ctcggtgcgc accacgtcgg cggcgcacga
      301 atcgattcag aatttcatcg acaacgtggt caacagcgcg cggcgccagg tgttgatcga
      361 ggcgaccgtg gtcgaggtta cgctcaacga cgactttcag gcgggtgtcg actggtcgcg
      421 gatcgcggtc ggcgacggct ggagcctgtc gcagaacgtg ctcgccgaaa ccatcggcgc
      481 ggcgaccagc ccggtcattt ccgcgaccta tgcgaacagc aacgacgagc gggatatcag
      541 tggggcgatc aaggcgctcg acgcgttcgg cgaggtatcg gtgatgtcga gcccgaagat
      601 catggcgctg aacaaccaga cctcgatcct caaggtagtc gacaacgtgg tctacttcac
      661 ccaggaaatc gatcgcgagc cggcgaccga caacagcccc gaggtgatca ccatcgatac
      721 cacggtcaac acggtgccgg tcggcttcgt catgaacgtc acgccgttca ttaccgccga
      781 agacgaaatt atcctcaaca tcaggcccac catatcgcgc gtactgggct tcgtcgcgcc
      841 gccatcgatc gtcataccag gcaccaccga gctgctcgag cagagcgcgg tgccacagat
      901 tcaggtgcgt gagatggagt cggtgctgcg cgtacaaagt ggcaataccg cggtgatcgg
      961 cgggctgatg caggataccg ccgacgagct gaatgccggc gtgcccgggc tgcacgatgc
     1021 ccagggtttc ggcctgctgt tcggtaccca gtcgcggcag tacgacaaga ccgaactggt
     1081 catcttcctg cggccgcggg tgatcgacaa tgccagcctc gataccaact taagggattt
     1141 cagcaaatac ctgaaacccc gggtgttcac gcctgagcag agtgactgaa tgagcttact
     1201 gctagacgcg ctgaaaaagg ccgccgagca gaaggcggca aaatcgaggc aggacgaatc
     1261 cgcggctgcc cgtgcgtcga atgccgggcc cgatgtgccc gggctcgagc ccgccggcgg
     1321 cgatgccgaa cccgggccgc cgtcacgcta tcgtcccggc gacgaagccg aacacgatga
     1381 ctccgcattc gatcctcgcg tcgagcctgc tgacgccggc gaccaggtca ctaccgaagg
     1441 tcgggaagac gaaaccctga tcttttcggt ggacgatgcc gccgagatcg tcgatgacga
     1501 cagcccgata gctgccccgg ccgacgacga taccgatctc aagccgctcg acggggtcgc
     1561 gggcggcgcc gatggtatcg atgtcgatga aatcgaggtg agccggctcg acggcgatga
     1621 aaccgacctc agtcagtttc tcgccgagca atccgccgcg gaccagggcg agaccggcag
     1681 tgccgatgcg ccgcgcctcg ataccgacga taccgatctg ggcctgcttg ccgcggccga
     1741 cgaagcggcg gtggaagctg ccgataatgc cttcgatagc ggacttgcgg aaggcggcga
     1801 taccgatttc gagctactcg aggtacccga cgacgaagat ctggagttgc agccgctcgg
     1861 cgatgatgag ctcgacttcg acctgttgcc gctggcggcg gacgcagacg aagcgagtga
     1921 tctcgacgag cagggcttcg cggccgggga tagcggcgcg ggcgccggcg acgcgggtgc
     1981 ggatgacgag gatatgtcgc tgttactgat cgagcgtgag ccgacgcaga tcaccagccc
     2041 gcgttcgacg acggccccgt ggtcgccgca ggatgcactg caatccctgc aggccgaggc
     2101 ccccaccgcg gcagatctcg ggctgatcga agccgcgcgc gagcggcctg ataagcagcc
     2161 tgccctgagt gaagacggaa cgcaaacccg gaaccggacc agcaatacgg ccaccaccca
     2221 gagtcgctcg agcgataccg ccactaccgg catcgccacc gccaccgcga acctcggcaa
     2281 ggtgccgcgc cccgatccca acgcggcgca aaaccatgcg ccggacaact acgaccgcac
     2341 gttgatgcgc ctgccgaacg aagaagcggc gcggctgttc gccgacatca cctccggttc
     2401 cgaggtggtg atgacgcccg attacgcgaa gcgagtgttt cgcagtaaat cgtcggcgtc
     2461 gcgcattcaa cacctgaaaa tgtacgtcgg catctcgatc gctatcctgc tggcgatcgc
     2521 tgtctacggt atcttcgaat accagaacga gtcggaaaac atcgaggccg agctgcgccc
     2581 gctgaagaac gacccgatgc ccggcatgct gcgcaccgag accgcaaata ccgaacccga
     2641 gacgatgttc gccgatgcag aggtcaacca gcgcgctctc gagatactgc aaaacgccga
     2701 aattaacgcg gagctgggga atgccggcgt ggcaacggcc gccgaaggca atgacgattc
     2761 cgaaatcgcc gccgacgaga cagccgccga gatggccatg gaaccgctgc cgtcgcccgc
     2821 gccgacgcac agcgctgaat cccctgctgc cgtcgaagac accgcaacct cgcaatccat
     2881 aatcctgtcc gaggtgtcgc gacaggcgcc cgcggagcgg gccactgtcg ccagcacggc
     2941 gacatcgggt tcgcaaacgc gcgccgcgac gagcggcgat tcgcgtttgc agatcgagac
     3001 cggcgagcgt ctcgaggaaa agcaggtatt gctgcgtgag gcctacggcg cttttcgagc
     3061 cggtgattat gcgcgcgccc tgaagggtta caatcgagtg ctggaaatcg atcccgccaa
     3121 ccgcaacgcg ctgctcgcgc ggggcgctat taatgtccac aatggtaatg gcgtggcggc
     3181 gataaatgac taccaggcgc tgttgctggc gaatcccaag gattcgctgg ccatggcctc
     3241 gttgctcgcg atcgccaatg tatcgccgct ggaaatcgaa actcagctca aactgatgat
     3301 ccgcgacgaa cccgattcgc cgtacctcaa tttcgccctt gccaatgtct acgcggcgca
     3361 ggatcgatgg caggaagccc agcgtcacta cttcaccgcg ctgcagaaca accctgacga
     3421 tccgaactat gcctataacc tggccgtcag cctggagcac atatcgcagc cccgggcagc
     3481 agtttcttac taccgtcgcg cgctggaaaa ctataataag ggtttagcga ccttcagccg
     3541 ggacgctgtg ggtcgaagac tggagaaact ggcaaactca tgaacgcgga aaacaagccg
     3601 aagcgtctcg gcgagattct gatggatcaa ggtgttatca ccgaagacca gctgaatatc
     3661 gccctcaccg agcagaaaaa ggttcatgaa cccctcggta agctgctggt cagtctcggc
     3721 ttcgtcaccg aggcgatcat gcgcgcggct ctcggcgaag tgctggaaca ggaaagtgtc
     3781 gacctgtcgc gcgtggtgcc ggacccggat gcgatcaaaa tgatcaattc cgataccgcg
     3841 cgcaaataca agatcatacc gctcaattac gatccgatcc gcggccgcct gacgatcgcg
     3901 atgtcggaca ccttcaacct gttcacgctg gatcgtattc gcatccagat cggccagcat
     3961 atcgagctgg tgccggtgct cgccggcgag gccgaaatct ccaacgcgat cgaccagttt
     4021 tacggttacg aactgtcgat cgacggtatt ttgcgcgaaa tcgagaccgg cgaggccgat
     4081 ttcaccaact accagggcga tatcgaggaa tacagccagc cgctggtgcg cctggtcgac
     4141 gtcatgcttt ccgacgcggt caagcgcggt gcctcggacc tgcacttcga accggaggaa
     4201 aactttctgc gcattcgcta ccgcatcgac ggcgtgctgc gccagatccg cagtctgcac
     4261 aagacttact ggtcggcgat cgcggtgcgg ctcaaggtta tggcggatat gaatatcgcc
     4321 gagacccgcg cgccgcagga tggccgcatc tcgcgtacca tcgttggccg gcagatcgat
     4381 ttccgggtcg cgtcgcagcc caccatccac ggcgaaaaca tcgtgctgcg cgtgctcgat
     4441 cgtgaaaagg gcgtcattcc tctggaaagc ctcgagctgc ccgacgaatc gctgcgcacg
     4501 ctgaagatca tgatgtcgcg tcctgaaggc atgatcctgg ttaccgggcc gaccggcagc
     4561 ggcaagacca cgacgctgta ttcactgctc aattacctgt ccagcgagca gatcaacatc
     4621 atgaccctgg aggatccggt cgagtatccg tttccgctga ttcgccagac ctcggtcaac
     4681 gaagccgccg gggtcggatt tgccagcggc attcgctcgc tgatgcgcca ggatccggac
     4741 gtcatcctgg tcggcgagat tcgcgacgag gatactgcgg aaatggcgtt tcgcgcggcg
     4801 atgaccgggc atcaggtata ttccacgctg cacaccaact cggcgatcgg cgcgctgccg
     4861 cgactgcgcg atatcggcat cattcccgag atcatggccg gcaacatcat cggcgtggtg
     4921 gcgcaacggc tgatccgcaa gctctgcaag tactgcaaga cggcctacgc ccccagcaag
     4981 gaagaactgc gcttgctgaa gaatgaccgc gacatcgtgc cgaagaaaat ttaccgcgcc
     5041 aagggttgca ccagttgtga caacaccggc tacaaggggc gcatggcatt gctcgagata
     5101 ttgaagatga attcggatct cgatgatttg ctcgcccatg gtgcgaccac caaggaaatc
     5161 aaggatgcgg cttacaagca gggtttcttc accctcgccg acgacggcgt gcgccacatt
     5221 ctcaacggcg tgacctcgat caaggaagtc gcgagggtcg ccgacctgac cgagcgcatg
     5281 ggttagccgt gccgctctac cagtacaagg caatcgacgc cagcggcaaa ttcatctctg
     5341 gtagtctcga cgcgggaaac atcaacgacc tcgagttgcg cctcgagaaa atggagatgg
     5401 acctggtcac ctacaagcag aaggaacccg gctccgacct gtttggccgc aacaagatca
     5461 gccgtcgcga cctgatcaat ttttcgtttt acctcgagca gttgacacgc gctggcgtac
     5521 cgatactcga agggcttacc gatctgcgcg acggcgagga aaacccgacc tttcgcgacg
     5581 tcatcaccgg cgtgatcgag gctatcgagg gcggaaattc gttttcccag gcgctcgggc
     5641 tgtatccgaa gatattcgac gaggttttcg tcagtctgat tcgtgtcggc gaacgcagcg
     5701 gcaagatgaa cgaagtacta atcgacatca ccgaaactct gaagtggcag gacgaactgc
     5761 tcgccaaggc gcgcaagatc atgatgtacc cgacggtgat cggcatactg gtgttgtcgg
     5821 tcgtcgtgtt tatgatgatc ttcatcgtgc ccgacatcat ggatgccatc gtcgcgctgg
     5881 gcggcgagat cccgctcgaa acccgcatgc tgatggcaac ctcggatttt ctggtccagt
     5941 tctggtacct ggtggtggcg tttccgttcc tggtgttttt tgttattcgg tatttctaca
     6001 atagcaatag ccgggttcgc ttcaaggtcg atggcatggc gttgaaggtg ttcatcctcg
     6061 ggccggtcaa cgaaaagatc aagatttcgc gcttttcacg ctatttctcg ctgatgttcg
     6121 cctcgggaat caccgtgctc gatgctatca acctgagcaa ggcggtggtc agcaacagcg
     6181 tgctcgaaga cggcatagaa cgggcctggc agcagatctc cgagggcagc agcatcagcg
     6241 aggcgttcaa gaatatcggc attttcccgc cgctcgtcgt gcgcatgctg cgcgtcggcg
     6301 aatccagtgg tcaaatggac aaggcgctga ataacgtcag ctatttcttc gaccgcgaca
     6361 tcaacgacag tatcgacaaa atggaaccgc tattgcaaac caccctgatg ctggttatcg
     6421 gcacggtggt gatgtggctg gccctggcgg tgctggggcc gatctacgaa accatcagca
     6481 ccatcgagtt ctagcggtcg aaagatgaag agagtccttt acttcaccgg ttatcgcatg
     6541 gttgcccagg aatggttggg ccgcaagctt gtcagcagcg tttacttcga gcctgacgag
     6601 cagggcctcg atctgttttc agcctattta cagtcgttca gtaacgagcc ggttcgaatg
     6661 ctggtcgatc tgatcgagga agagtttcgc cagatacgcg tcccgctgct gcgcggttcg
     6721 gaccgccagg cgattctgaa acgcaactat gccaagtact tccggcattc ggattataag
     6781 ttcgcgatct cccaggcggt ggagaaaaag acccgcaagg aagaaaagct gctgttgatc
     6841 ggcctcacca accagtacct gctcgagccc tggctgaaaa tcatcgagca gtcgcaaacc
     6901 ccgctgtcgg gaatcatcag cctgccactg ctctccgagg actacgtcaa ggaactcaag
     6961 tcggcccatc gagcgatcat tctggtcagc cagcaggtgc caagcaacct gcgccagtcg
     7021 gtgttcgtcg acggcaggct ggtgctcagc cgactggtgc cgatcgcgag cttttaccag
     7081 ggtgactatg cgtcggactt gctgcgcgat atcgagtcga cgcagcgcta tttgatcaac
     7141 cagcgcatcg tggagcgcgc ggagaccatg gcggtacaca tcctgaccaa caagcggcat
     7201 ctcgaaaggc tgcagtcgcg ctgccagcag gagacccagt acgacttcac gattcacgag
     7261 atcaacgagc tgctggcacg cgaaaaaatc gatgtcagtg acgagcagga tttcagttcc
     7321 ggcctgttct gctacctggc cagtcgcaag atcacgctga accattacgc ccaacccagg
     7381 gagcggcgct tctttcagta ccatcgcgcc ggtctcgcgc tgcgcgcgct cagtatcgcc
     7441 ctgattgccg ttggcgccgg gttcgcgctg aacggcgggg tcgcgaactt gctttataag
     7501 cagacgatcg aggaaacccg gctcatcgag caaaaatacc gctccaagta caaccagttg
     7561 agccagaacc gggtggattc gaatacctcg acaagcagca tgcagtacat cgtgcaaacc
     7621 gtcgatcaga tcgaaaaccg ctacctgcgc gatccggagg aattgctggc gatgatcagc
     7681 gaagacatca gcattttccc cgatattcgc gtcaggcggc tcgactggtt cgtctccaat
     7741 tcctccgctg ccgtcgatgc cggcgacgta tcctgggggc ggtcgggaca aaccggcaat
     7801 cgcagctcca gccgcgatcg aaacaagccc gccgcgaaaa ccggatattt tgaaattgtc
     7861 agactggagg gcgagtttct taatttcgac ggtaatttcc gctacgcgct gagtgcggtc
     7921 gacgatctcg agaaagccat gaccgagtcc ggcaagtatt actcggtcga aatcaccaag
     7981 cggccgctag acatcgaatc cgataaccgc ctgagcggtg acgccggcgt ctttggcggc
     8041 agtcaaagat cgttgcgcgc ggaactggct ctgcgcgtgg ttcgggaggt tccgcaagtt
     8101 gaataaggtc gtctggaaat cattgcgctg gccgctgatc atattcgtga tcgcgctggt
     8161 gattgcctcg gcgttcggtt acttcggtat gcacctcgag gattccgcgc agcaggatta
     8221 ccagcgggca gtttcgtcac tgcgctcgac gcaccagcgc taccgtgagc tggtcaatga
     8281 catcgacctg ctggagcaat accgcaaccg ctataccgaa tacaaggatt ccggcctggt
     8341 cggcggcgag cgccgcctga gctggatcga aagcctcgag gctaccaatg gcgtgctgcg
     8401 cctgccgacc ctgacctaca acctgcatcc gcaggaaggt ttcgagcggc ccggtttcag
     8461 tgtgcagcag ggcgtgatgg tgcagagttc gccgatggac ctgtcgatgg acctgttgca
     8521 cgaggaagac ctgtttgcct tactcgaggg cttgcgcctg tcgatcagca acctgtttac
     8581 ggtcgacagc tgctcggtca accgggtgtc cggaatcggc agttcgctcg agacccggga
     8641 caccaacctg cagggcaggt gcatgattcg ctgggtgacg atcgatgtcg gttaagcgcc
     8701 tcgtcctcgt cctgttgctg tcgtacggca tacagctgcc ggcattggcc gcggaactgg
     8761 cgaccctgtt tacaaccccg caggagcgcc gcctgatcga caggaatcgt tacaagcagg
     8821 ccgaagccga gccccaaccg cagcccgccg cggatcctgg cgacgtattc gcggtcgacg
     8881 acgttgaata caccctcgaa gaggtaaccc gcgaataccg ggtttccggc atcactctgt
     8941 cacgggaagg gtcgcacacg gtatggatca atgccaaagc ctacgaggac ggcgcgcggc
     9001 tcgatgacga cagccgtatt caggtgatcg ccggcgatca gatcagggtc aggataaccg
     9061 cgcccgacgg caggcaatac ctggccgcca gcggcgagac ggtcgagatc agtttgctcg
     9121 tgccggtgga gtccgaatga tgagacccgg atattcgcag agcggttatg ccttgctgct
     9181 ggtgttgctg gtgctgatgg gtgccggcgg cgtggtgctg gccgggttca cgcagcaggt
     9241 caagcacgag gccgagcggc agcgttacga acacaatcag cgcgtgctta gggaagccaa
     9301 gcaggcactg ctggtgtacg cctaccgtta tccagatatc gcgctggctt tcaacg
//
LOCUS       DATCZV010000354         7074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_399468, whole genome shotgun sequence.
ACCESSION   DATCZV010000354 DATCZV010000000
VERSION     DATCZV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399468"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="cas6"
                     /locus_tag="VIW27_07975"
     CDS             <1..695
                     /gene="cas6"
                     /locus_tag="VIW27_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HEY5739649.1"
                     /translation="RMFWREDKRPEQAFRVPDDVCDLVFRLSGKRIDVDHAHALAEAL
                     REHLDDDTCARIGVHGVRMASSGNGWNRPEGAGAELPLSRRARLDIRVPRELVDTVLR
                     LSNSRLALGSGEIEVGGGTVRPLSSHGTLHARAISCDRQQSEDEFLRQVAAELQAMQI
                     DVSRMLCGRSAEIRIAGGSLFTRALMIADLKPRESVRLQQLGIGGGRLLGCGLFVPHK
                     EIDAVYEPQEQD"
     gene            728..1069
                     /locus_tag="VIW27_07980"
     CDS             728..1069
                     /locus_tag="VIW27_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEY5739650.1"
                     /translation="MSLNVDGREIPITDSGFLENVEDWSRQVAEVIAVEEQLELTERH
                     WDLINHLRDEYINNGGNQPNTRSLVKSMSRAWNDKSVSAKTLYDLFPGDPSKQGGRIA
                     GLPESRRKGGY"
     gene            1114..1317
                     /locus_tag="VIW27_07985"
     CDS             1114..1317
                     /locus_tag="VIW27_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739651.1"
                     /translation="MSNKQEIIKQMLEMQRRFIELEQNGEFSIEDYYDSGGDSELAQF
                     KRTYDELATQLVDLAHADKGSHR"
     gene            complement(1330..1830)
                     /locus_tag="VIW27_07990"
                     /pseudo
     CDS             complement(1330..1830)
                     /locus_tag="VIW27_07990"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
     gene            complement(1855..2025)
                     /locus_tag="VIW27_07995"
     CDS             complement(1855..2025)
                     /locus_tag="VIW27_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739652.1"
                     /translation="MHRVFLIIMLVTIPDLLDDTQLAAIRAELDRGHFVDGKLSAGQR
                     AAVDKRNLELAP"
     gene            2020..2343
                     /locus_tag="VIW27_08000"
     CDS             2020..2343
                     /locus_tag="VIW27_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739653.1"
                     /translation="MQLIVEIDDKSYPLDVPEALCLEAADFFEKMDRDMSCGWQMSRW
                     WVPDPDLLQRCQIAADKLLTAMQKDNLRMAQMMGAYILSKRPATRRIRLNVDGEIQGN
                     EFFDE"
     gene            2430..3350
                     /locus_tag="VIW27_08005"
     CDS             2430..3350
                     /locus_tag="VIW27_08005"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5739654.1"
                     /translation="MPNDQRLTGVLAPVVTAFDANLDPDVERWVRHCRWLVDNDVGLA
                     VFGTNSEANSMSVAEKLELLERLVEAGLPPRRMMPGTGCCALPDTVELTRKAVELGCA
                     GVLMLPPFYYKGVSDDGLYRSYAEVIERVADARLRIYLYHIPQIANVPITLTLIERLL
                     RDYPKIIAGVKDSPGDWDNTLAMLERFQPHGFDVFCGSESFLLETLRHGGAGSITATA
                     NVNPAPIARLCREFEQAGAERLQAELDAVREIFQGYVMIPGMKAALAHYLDDPQWSRV
                     RPPLDETGDGQREQLVSALRKIGFEITGLA"
     gene            complement(3464..4048)
                     /locus_tag="VIW27_08010"
     CDS             complement(3464..4048)
                     /locus_tag="VIW27_08010"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY5739655.1"
                     /translation="MSAKSIIEIRPGSFVFEQRAALPTDVCAEMIRRFEEYTAEQYPG
                     RIGQTHEFDQSIKRSTDLVISGKSHWQDLDRELFRSLSHALAEFRQSFDFFGGPFKDN
                     GYAIQRTDAGEFYHWHIDGGSHEFALRQLVAIWYLNEPQGPGGETEFRYQDLSVSPET
                     GKLLMFPPFWTHLHRGKTLERGHKYIATTWVVFA"
     gene            4183..4968
                     /locus_tag="VIW27_08015"
     CDS             4183..4968
                     /locus_tag="VIW27_08015"
                     /inference="COORDINATES: protein motif:HMM:NF023472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HEY5739656.1"
                     /translation="MTNKVFTAAIAILLSLSTTWVYAATLSDTAKEARWAEQVVPSLL
                     DGDAVMLDDGQGHEFLGIYTPADGEKRAVILVHGIGVHPNWPDVIYPLREALLENDIS
                     SLSIQMPILENEATEAEYLPLYDEAPGRIQAAVDFLKDAGYEKIDIAAHSMGSRMTVY
                     FLSSEGAREVNSVVLIGMGESGGDVWTKVLDTLADLRMPVLDLYGEKDLENVLTSAQQ
                     RAESGSKNKSGIFRQIEVEGANHFFQGHEDELKSAVMDWLQSS"
     gene            4994..5566
                     /locus_tag="VIW27_08020"
     CDS             4994..5566
                     /locus_tag="VIW27_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739657.1"
                     /translation="MVRVKKRWNQQARPRTLAQMANAIGASIWKLAANVVLNLENENF
                     ETDTQVQRVEIMEEVVCYLVHIADRRVYAQASQSQRAEFIAALVADLARMLEDSRCDV
                     QGAGEYQAAFIDKVNQRSAEYASFGFSDEDGASFSMRCRLGDRVAASMGERDRRWIPD
                     YLVGREAPEIEAALQKSLAGLVSFKTDEAD"
     gene            complement(5618..5815)
                     /locus_tag="VIW27_08025"
     CDS             complement(5618..5815)
                     /locus_tag="VIW27_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739658.1"
                     /translation="MNQAYRDAIAQMEEMNVQAEYVLGWQGGFLGHPLREEQRITEAY
                     QAGYDYGQEKSTENFSNWVEK"
     gene            complement(5933..>7074)
                     /locus_tag="VIW27_08030"
     CDS             complement(5933..>7074)
                     /locus_tag="VIW27_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY5739659.1"
                     /translation="LDLYTQYLWWAFGLAFIFGAIANRANFCTMGAVSDWINIGDLSR
                     MRSWMLAIATAIVGVGILEYTGSIDLSLTTSNETSNPPYRAANFIWLRHLLGGLMFGV
                     GMTLSSGCGNKTLVRLGEGNLKSLVVLVIMGIAAWWMLFTNLSFNAFLSWMLPLSIDF
                     SQQQIPRQDIAAVLSSLTGIEAGAATGLVVALIVALPLAFWILRAPDFRENLELLAAG
                     VVIGLLIVIGWYVTAGPKGLLLLEELDFADQRPFFTGAQSMTFIGPAGHVVQYVKQGF
                     GMVYLTFGVATIAGVVVGSFVYTLVFRKVRLEWFVSWQDFGAHVAGALLMGIGGVLAM
                     GCTIGQGITGVSTLALGSILTILAIIAGSALTMKYQYYRMMREDD"
BASE COUNT         1456 a         2053 c         2268 g         1297 t
ORIGIN      
        1 agcgcatgtt ctggcgagag gacaagcggc ccgagcaggc tttcagggtg cctgacgatg
       61 tctgcgatct ggttttccgg ctcagcggca agcgcatcga tgtcgatcat gctcacgcgc
      121 tggcggaagc cctgcgcgag catctcgacg acgacacctg cgcgcgcatc ggcgtgcacg
      181 gggtgcgcat ggcgagttcg ggcaacggct ggaaccgtcc cgagggagcc ggcgccgaat
      241 tgccgttgtc gcggcgtgcg cgtctcgaca tccgcgtgcc gcgggagctc gtcgatacgg
      301 tcctgcggct gagcaacagc aggctcgccc tcggcagcgg cgagatcgag gtcggcggcg
      361 gcactgtgcg cccgctgtcg agtcacggta ccttgcatgc acgggcgatc agttgcgatc
      421 ggcaacagtc ggaggacgag ttcctgcggc aggtggctgc cgagttgcag gcgatgcaga
      481 tcgacgtgtc gcgcatgctt tgcggcaggt ccgcagagat tcgcatcgcc ggcggaagcc
      541 tgtttacccg ggcactgatg atcgccgacc tgaagccgcg cgaatcggtc aggctgcagc
      601 agctcggtat cggcggcgga cgtctgcttg gctgcgggtt gttcgtgccg cacaaggaaa
      661 tcgacgcggt ttacgagccg caggagcagg attaataatc ttgggcaaca atatacgagg
      721 ttgaaacatg agtctgaacg ttgacggcag agaaattccc attaccgata gcggatttct
      781 ggagaatgtc gaggactggt cgcggcaggt ggcggaggtg atagccgtcg aggagcaact
      841 ggaactcacc gagcggcatt gggatctgat caatcatctg cgtgacgaat acatcaacaa
      901 cggcggcaac cagccgaata cgcgaagcct ggtgaagtcg atgtcccgcg cctggaacga
      961 caagtcggtc agtgccaaaa ccctgtatga tctgtttccg ggcgatccca gcaagcaggg
     1021 cggcagaatc gcaggcctgc cggagagccg cagaaagggc ggctattgac ccggcctgag
     1081 aatgcgcgcg aacctattca cagaggtact caaatgagca ataaacagga aattatcaag
     1141 caaatgctgg aaatgcagcg gcgcttcatc gagctcgagc agaacggtga attttcgatc
     1201 gaggattact acgacagcgg gggcgactcg gagctggcgc aattcaagcg cacctacgac
     1261 gaactggcga cgcagctggt cgaccttgcg catgccgaca agggctcgca ccgctaacgg
     1321 cgggtgagac tatatctccg cccacatgcg gatcaggttc gcgtagcaca ggtcgaggtg
     1381 ttgcagggcc gcggcctggc tttggcccgc cagggcctcg cgggcgcggt cgagccgaag
     1441 caggatttcg cgttggtcgg cccgccgcac caggctttgc acccaggtca ccgcgaccag
     1501 ccgttcgccc gccagcagcg gcttgaccgc gtgatagcgg ctcgccggat aaagcaccgc
     1561 atgcccggcc ggcaacttga cttcgcggtt gccgtgcggc aattcgatga gcagttcacc
     1621 cccctcgtac tcgtgcgggg aattgagaaa aatcgtgatc gcgatatccg cgcggtaacg
     1681 cgccgcgggc gggcccatga ccggatcgtc gatatggtag ccgtattcca tgccggccgt
     1741 gtagcgcgcg tagatcggtg cgcagatttt cgccggcagt gccgcctgca ggtagagcgg
     1801 gttttgcacc aggcgggtca tgacgacgtt gtttagtgcc tcgaaactgg cggcttaggg
     1861 tgccagttcg aggttgcgct tgtcgactgc ggcccgctgg cccgcggaga gcttgccgtc
     1921 gacaaaatgg ccgcggtcga gctccgcgcg aatcgcggcg agctgcgtgt cgtcgagcag
     1981 gtcgggaatc gtcaccagca tgattatcag gaaaacccta tgcaactaat tgtcgagatc
     2041 gatgataaga gttatccgct ggatgtgcca gaagcgctgt gtctggaggc cgcggatttc
     2101 ttcgaaaaaa tggacaggga catgagctgc ggctggcaga tgagccgctg gtgggtgccg
     2161 gaccccgatt tgctccagcg ttgccagatc gccgccgaca agctgttgac cgcgatgcag
     2221 aaggataacc tgcggatggc gcaaatgatg ggcgcctata ttctcagcaa gaggcccgcc
     2281 acgcggcgca tccgcctcaa cgtcgacggc gagatccagg ggaacgagtt tttcgacgag
     2341 tagttgcctc agcgagacgt cgtgaggtaa attcggcttg ccgcaagcaa acccggcttg
     2401 aagacgcaga cagcgaggag tagcgccgca tgcccaacga tcaacgtcta accggtgtac
     2461 tggcgccggt agtcacagct ttcgatgcca atctcgaccc agatgtcgag cgctgggtgc
     2521 gccattgccg ctggctggtc gacaacgacg tcggcctggc agtgttcggc accaactcgg
     2581 aagccaactc gatgtccgtg gcggagaagc tcgagttgct cgaacggctg gtcgaagcgg
     2641 ggctgccgcc acggcgcatg atgcccggca ccggctgctg tgcgttgccg gacaccgtcg
     2701 agctgacgcg caaggccgtc gagctcggtt gtgccggcgt attgatgctg ccgccgtttt
     2761 actacaaggg ggtcagcgac gacggtcttt atcgaagcta cgcggaagtc atcgagcgcg
     2821 tcgccgacgc acggctgagg atttacctat accacatacc gcagatcgcc aacgtaccga
     2881 tcacgctaac gctgatcgag cgcctgctgc gggattaccc gaagattatc gccggcgtca
     2941 aggacagccc cggcgactgg gacaataccc tcgccatgct cgagcgtttc cagccacatg
     3001 gtttcgatgt cttctgcggc tccgagtctt ttctgctcga aaccctcagg cacggcggtg
     3061 cggggtcgat caccgccacc gccaacgtca acccggcgcc gatcgccagg ctgtgccgcg
     3121 agttcgaaca ggccggcgcc gaacggctgc aggccgaact cgacgcggtt cgggagatct
     3181 tccagggcta tgtgatgatc cccggcatga aggccgcgct ggcccactat ctcgacgacc
     3241 cgcaatggtc gcgggtgcgc ccaccgctag acgagactgg cgatgggcaa cgcgaacagc
     3301 tggtttcggc gttgcggaaa atcggcttcg aaatcaccgg gctggcttga ccccggatgg
     3361 cagtgggctg gcagcagtcc tgtttcatgg cgctgcgcgg agagcggccg gacgggcaat
     3421 tcgtcaccga tggccacgtc gcccgctgca ccgcgcaccg ggttcaggcg aataccaccc
     3481 aggtggtggc gatgtacttg tggccgcgct cgagggtctt gccgcgatgc aggtgggtcc
     3541 aaaagggtgg aaacattagc aatttgccgg tctcgggact aaccgacagg tcctggtagc
     3601 gaaactcggt ctccccgccc gggccctgcg gctcgttgag ataccagatg gcgaccagct
     3661 ggcgcagcgc gaattcgtga ctgccgccgt cgatatgcca gtggtagaac tcgccggcgt
     3721 cggtgcgctg gatggcataa ccgttgtcct tgaaaggccc gccgaaaaag tcgaagcttt
     3781 gtctgaattc ggcgagcgcg tggctcaggg agcggaacag ttcgcggtcg aggtcctgcc
     3841 agtgggattt gccgctgatc accaggtcgg tggaacgctt gatcgactgg tcgaactcgt
     3901 gagtctggcc gatacggccg gggtattgct ccgcggtgta ttcctcgaaa cggcggatca
     3961 tctcggcgca gacatcggtc ggcaacgcgg cgcgttgctc gaagacgaag ctgccgggcc
     4021 tgatttcgat gatggatttg gcgctcattt gacgtgctgc atgctttgcg gcaattttag
     4081 cctgcccgct ggcggacgac atacccaagt tgggtagaga agagaaatct ccaacgcgat
     4141 atcatctgcg gttcgggtac cacttgacgg gagtgaagaa caatgaccaa caaggttttt
     4201 actgctgcaa ttgcgattct gctgtcgtta tcgacgacct gggtgtacgc cgcgaccctg
     4261 tccgataccg cgaaggaagc ccgctgggcc gagcaggtcg tcccgtcgct actagacggt
     4321 gacgctgtca tgctcgacga tggccagggg cacgaatttc tcggcattta taccccggcg
     4381 gacggcgaaa agcgggcggt gattctcgtg cacggtatcg gcgtgcaccc caactggccc
     4441 gacgtgatct acccgctgcg cgaggcgctg ctggaaaacg acatcagctc gctgtcgatc
     4501 cagatgccga tcctggagaa tgaagccacc gaagcggaat acctgccgct gtacgacgaa
     4561 gcccccggcc ggatacaggc cgcggtcgat ttcctcaagg acgccggcta cgaaaagatc
     4621 gatatcgctg cccacagcat gggctcgaga atgacggttt attttctttc cagtgaaggt
     4681 gccagggagg taaattcggt ggtactcatc ggcatgggtg aaagcggcgg cgatgtttgg
     4741 acaaaagttc tcgacacgct cgcggatttg cgtatgccgg tgctcgatct ctacggcgaa
     4801 aaagatctcg aaaatgtttt gacctcggca cagcaacgtg ccgaatcggg aagcaagaac
     4861 aagtctggaa ttttccggca gatcgaagtc gagggcgcca atcatttctt ccaggggcac
     4921 gaagacgagc tgaaaagcgc agtcatggac tggctgcagt ccagctagac ggtaaataac
     4981 gcgcggacga ggcatggttc gggtcaagaa acgctggaac cagcaggcca ggccgagaac
     5041 gctggcgcaa atggccaatg ccatcggcgc cagcatctgg aaactggccg ccaacgtggt
     5101 gctcaacctc gaaaacgaga atttcgaaac cgacactcag gtgcagcgcg tcgagatcat
     5161 ggaagaagtc gtctgttacc tggtgcacat tgccgatcgc cgagtgtatg cgcaagccag
     5221 ccagtcacag cgcgccgaat tcattgcggc gctggtcgct gatctcgcac gcatgctcga
     5281 agacagccgc tgcgacgtgc agggcgcggg cgagtaccag gcggcgttta tcgacaaggt
     5341 caaccagcgc tcggccgagt acgccagctt cggcttcagc gacgaggacg gcgcgagttt
     5401 cagcatgcgc tgccgtctcg gcgaccgcgt ggcggcgtcg atgggcgagc gtgaccggcg
     5461 ctggattccg gattacctag tcggcaggga agcgccggag atcgaggcgg cgctgcaaaa
     5521 atcgctggcg ggactggtgt cgttcaagac cgacgaggcg gattgaatcg gcctcgtccg
     5581 caggttgtcg ggcgttacgc gaatcgtttg accgcgacta tttttcgacc cagttagaaa
     5641 aattctcggt ggacttttcc tggccatagt cgtagccggc ctggtaggcc tcggtaatac
     5701 gctgttcttc gcgcagcgga tggccgagaa atccgccctg ccagccgagc acgtactcgg
     5761 cctgcacgtt catctcttcc atttgcgcaa tcgcgtctcg ataagcctgg ttcatcgaaa
     5821 tctcctgtaa actcgtcttc ggttcgaatt cgtacccgca agcaaggaag gccgccgggg
     5881 tccagctagt gggctagcct agcaaaaccg ccagtgagcg ggtatatccc aactagtcgt
     5941 cttccctcat cattcggtaa tactggtatt tcatggtcaa cgcgctgccg gcaatgatcg
     6001 ccagaatcgt caggatcgaa ccgagcgcca gtgtcgacac cccggtgatc ccctggccga
     6061 tcgtgcagcc catcgcgagc acgccgccga ttcccatcaa cagcgcgccg gccacgtggg
     6121 cgccgaaatc ctgccaggaa acgaaccatt cgaggcgcac cttgcggaag accagggtat
     6181 acacaaagga gccgaccacg acgccggcaa tggtggccac gccgaaggtc aggtagacca
     6241 tgccgaatcc ctgcttgacg tattgcacca cgtgaccggc ggggccgatg aaggtcatcg
     6301 actgcgcgcc ggtaaaaaac ggcctttgat cggcgaaatc cagctcttcc agcagtaaca
     6361 ggcctttcgg cccggcggtt acgtaccagc cgatcacgat cagcaggccg atgaccactc
     6421 ccgcggcgag caattccagg ttttcgcgga aatcgggtgc acgcagaatc cagaacgcca
     6481 acggcagggc gacgatcagc gcgaccacta atccggtggc ggcgccggcc tcgataccgg
     6541 tcaggctgga cagcactgcg gcgatgtcct ggcgcggaat ctgctgttgt gaaaaatcga
     6601 tgctcagcgg cagcatccag ctgaggaaag cgttgaagct aagattggtg aacagcatcc
     6661 accaggcggc gatgcccatt atgacgagca ccacgagcga tttcaggttg ccttcgccga
     6721 ggcgcaccag ggtcttgttg ccgcagccgc tcgacagggt catgccgacg ccgaacatca
     6781 gaccaccgag caggtgacgc agccagatga aattcgccgc gcggtagggc gggttggagg
     6841 tctcgttact ggtggtcagc gataaatcga tgctgccggt atactcgagg ataccgacgc
     6901 cgacgatagc ggtcgcgatc gccagcatcc acgagcgcat gcggctaagg tcgccgatat
     6961 tgatccagtc ggaaaccgct cccatggtgc agaaattcgc gcggttggcg atcgcgccga
     7021 aaatgaaggc cagcccgaag gcccaccaga gatattgtgt gtaaagatct agct
//
LOCUS       DATCZV010000355         3813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_452647, whole genome shotgun sequence.
ACCESSION   DATCZV010000355 DATCZV010000000
VERSION     DATCZV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452647"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1245
                     /locus_tag="VIW27_08035"
     CDS             130..1245
                     /locus_tag="VIW27_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HEY5739660.1"
                     /translation="MAAARTRLILDKPFLGALALRLPLVEAAEDSCRSTRCDARNFYY
                     NRAYIDALDIDETQFALSRESLHCALLHFYRRGNRQPGLWDRACDLAVSSILIRDGLK
                     PTPDTVYRPEFDGMTAEEIYPLLVDEAAGSAAPERPAGAGDRESQSSPRPIEQSLRPS
                     EIEILATQWQQRLAAAAQQAMQAGKLSAELARIVDFFLQPKLPWRSLLAQYLSATARN
                     DYSYARPSTRRGDPAVFPGLRSDEIDLVVAVDTSGSIREAEIEEFFAEINAIKGQIRA
                     RIALLCCDAGIAAGFPMFFEPWEDFDFEPRVSGGGGTDFRPVFEWVERQDQRPDSLVY
                     FTDACGEFPDHEPPYSTLWLVKGRQDVPFGARIQLNE"
     gene            complement(1427..3457)
                     /locus_tag="VIW27_08040"
     CDS             complement(1427..3457)
                     /locus_tag="VIW27_08040"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEY5739661.1"
                     /translation="MTQSPDDRLGIMPVEARKPDSTDAPFFRDTLVAGIFIASLALYL
                     LSMPKTVALEDDSIFILSGYFNGVSHPPGYPLYTLLLHLFIQIPIGDIPARAHASSAF
                     FAALACCTLFYIFCLAGLERRLAALGALVFAISSTFWSQAIIAEVYSLNVLLNLSLLL
                     LGLRIHLHCTDKTVPAKALTRDFLLFSIAMGLAISNHWPLTVLAAPAYLLLVARPFFS
                     LRNKLIAVLPGLAVAAGFYAWLYFNNQSAPFINFSGKFTGLKEFVDFVLRSHYSTVDY
                     QPTVGWSDKIQFSRDLLLQAARDLNLLLIFAALGFYRLVKSPDTLIVGLAVGWAVFSN
                     SILLVLLVDFDYSEIFSVVFQVYPIVSIAMLFVLAGYGITAVNTLLRIDVTRIHLVVV
                     LLAALVLNAMVSIPQNYRHDYSWGEEFAQKIFEQVPESATLFSAGDVEMGLLSYYHFV
                     EGRRPDIRLYSSTALLLDNRLFDYRLADKKTFIEALVQENPQQEFYVANNYYDLEKQS
                     SNLYVDRLGKPETLAQHSITVQDVELLLQWSAPEYTRDPWTRYAIAELRKRAIFIMTA
                     AMKNAADAGLKEYLSTSIASLIRTNGDALHFLKNLIKDASTISPGFYQARLADIDRGQ
                     LRSKREDSLYVYLAARASQAEQTREHIENSQRKACLNWPSSKNIYCQRQDLD"
     gene            complement(3454..>3813)
                     /locus_tag="VIW27_08045"
     CDS             complement(3454..>3813)
                     /locus_tag="VIW27_08045"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY5739662.1"
                     /translation="VGATGVLVQLLFVWLLHIVVTADFVIATVIATGIAMSSNFVLNN
                     WFTHYDRRLTGIALIKGYLSFVLACSVGATINVFVANLSFENLGVWWIASLLGAVFGS
                     IWNYTMSNILTWRPRQA"
BASE COUNT          803 a         1061 c         1207 g          742 t
ORIGIN      
        1 ggcgtgatgc tggtcgccga cctgcatcgg gccatcgggc cagcattgtt cgcggtaccg
       61 cagtttgccg actgggccga gcaggtcggt gatatcctgg tttactgagc cttggacacg
      121 atcaccaagt tggccgccgc ccgaactcgg ctgatcctcg acaagccgtt tctcggtgcg
      181 ctggcgttgc ggctgccgct ggtggaggcc gcggaagaca gctgccggtc gacccgctgc
      241 gatgccagga atttttatta caaccgcgct tacatcgatg cactggatat cgacgaaacc
      301 cagttcgcac tgtctcgcga aagcctgcat tgcgcgttgc tgcactttta ccggcgcggc
      361 aaccgccaac ccgggttgtg ggacagggcc tgcgatctgg cggtgagctc gatcctgatt
      421 cgcgacggcc tgaaaccgac ccccgatacc gtttaccggc ccgaattcga cggcatgacg
      481 gccgaggaaa tctatcccct gctggtggat gaggcagccg gcagtgccgc gccggagcgg
      541 cccgccggcg caggcgatcg cgagtcgcag tcgtcgccgc ggccgatcga gcaaagcctg
      601 cgaccgagcg aaatcgagat cctcgccacg cagtggcagc agcgactggc cgcggcggca
      661 cagcaggcga tgcaggcggg caagctgagc gcggagctgg cgcgcatcgt cgattttttc
      721 ctgcagccga agttgccgtg gcgcagcttg ctggcacagt acttgagcgc tactgcgcgc
      781 aacgactaca gttatgcccg cccgtcgacc cggcggggcg acccggcggt gtttccgggc
      841 ctgcgcagcg acgagatcga cctggtggtc gcggtcgata ccagcggctc gattcgcgag
      901 gcggagatcg aggagttttt tgccgagatc aacgcgatca agggtcagat tcgcgcgcgc
      961 atcgcactgt tgtgctgcga tgccgggatc gccgccgggt ttccgatgtt tttcgagcca
     1021 tgggaggatt tcgatttcga gccgcgggtc agcggcggcg gcggaaccga ttttcgcccg
     1081 gttttcgagt gggtcgagcg acaggaccag cggcccgatt cgctggttta cttcaccgac
     1141 gcctgcggcg aatttcccga tcacgagccg ccgtactcga ccctgtggct ggtcaagggc
     1201 cgccaggacg tgccgttcgg cgcgcgcatt caactcaacg aataagcacc gggatcgaat
     1261 gggccgggtc gccgggcgcg gtatcatctg cggggataaa acgggcgaca ggcgatctta
     1321 atcttgcccg tttgcggtga tcgattcgag gtcgtggtgt tatcgagatg gggtgtattt
     1381 ccgctttccc cgctaccccg acagcaaagc tatcgagaga ttgcgactag tcgaggtctt
     1441 gccgctggca atagatgttt ttcgaagatg gccagttcag gcaggctttg cgttgcgaat
     1501 tctcgatgtg ttcccgggtt tgttcagcct ggctggcacg ggccgcgagg taaacataca
     1561 gtgaatcctc gcgcttggag cggagctgtc cacgatcgat gtcggcgagc cgggcctggt
     1621 agaaaccggg actgatcgtg gatgcgtctt ttatcaggtt tttcagaaaa tgcagggcgt
     1681 cgccattggt tcgaatcagg ctggcgatag aagtcgacaa gtactccttg agcccggcat
     1741 cggcggcatt cttcatcgcc gcggtcatga tgaagatggc tctctttcgc agctcggcga
     1801 tggcatagcg cgtccacgga tctcgagtat attcgggcgc gctccactgc agcaacagct
     1861 cgacatcttg cacggttatg ctgtgctgcg ctagcgtctc gggctttccg agcctgtcga
     1921 catacaggtt tgacgattgc ttttcgaggt catagtaatt gttggcgaca tagaattcct
     1981 gctgtggatt ttcctgcacc agcgcctcga tgaatgtctt tttatcggcc agccgatagt
     2041 cgaacaggcg attgtcgagc aacagggccg tcgaagaata aagcctgatg tcgggtcttc
     2101 gaccctcgac gaaatggtaa tagctgagca ggcccatctc gacgtccccg gcggaaaaca
     2161 gcgtcgcgga ctcgggaacc tgctcgaata tcttctgtgc aaattcctcg ccccaggaat
     2221 agtcgtgccg atagttctgc ggtatcgaaa ccatggcgtt taataccagg gcggcgagca
     2281 gcacgacgac caggtgtatg cgggtgacat cgatccgcag tagggtattg accgccgtga
     2341 tcccgtagcc ggcgagcacg aacaacatgg cgatcgaaac gatcgggtag acctggaata
     2401 ccaccgagaa gatttcgctg tagtcgaaat cgaccagcag gacgagcaag atgctgttcg
     2461 agaagactgc ccagccgaca gccaggccga cgattagcgt atcgggggat ttgaccaggc
     2521 gataaaaacc gagggcggca aatatcagta gcaggttcag gtctcgcgcg gcttgcagca
     2581 gcaggtcgcg gctgaattgt atcttgtcgg accagcccac ggtgggctga tagtccaccg
     2641 tggaataatg gctgcgcaat acgaagtcga cgaattcttt caggcccgtg aacttaccgc
     2701 tgaaattgat gaacggagcc gactggttat tgaaatacag ccaggcgtaa aagcccgccg
     2761 ccaccgccag tccgggcaat accgcgatca gcttgttcct caagctgaaa aatggcctgg
     2821 caaccagcag caggtaagcg ggagcggcca gcaccgtcag tggccagtgg ttggaaatcg
     2881 ccaggcccat cgcaatcgaa aacagcagga agtcgcgggt cagcgccttt gcaggcacgg
     2941 tcttatctgt gcaatggagg tggatgcgca agcccagcaa cagcaggctc aggttgagca
     3001 agacgttgag gctatagact tcggcgatga tggcctgtga ccagaaggtg gaagatatcg
     3061 cgaataccag ggcgccgagc gccgccaggc ggcgctcgag tccggccagg caaaaaatgt
     3121 agaacagggt gcagcaggcc agcgccgcga agaaagcact gctggcatgg gcccgtgcgg
     3181 gtatatcgcc gatcgggatc tggatgaaca agtgcagcag cagggtgtac agggggtacc
     3241 cgggtggatg agagacgccg ttgaagtacc ccgagaggat gaaaatactg tcgtcctcga
     3301 gcgccacggt tttcggcatc gacagcaaat acagggcaag gctggcgata aaaatgcctg
     3361 caaccagcgt atctctgaaa aacggcgcgt cggtcgaatc gggttttcga gcttcaaccg
     3421 gcatgatccc gagccggtcg tcgggagatt gcgtcatgcc tgccggggtc tccaggtcag
     3481 gatgttggac atggtgtagt tccagatcga gccgaacacg gcgccgagca ggctcgcgat
     3541 ccaccagaca ccgagatttt cgaagctgag attggcgaca aaaacattaa tcgtcgcacc
     3601 cacggaacaa gccagcacga agctgagata acctttgatc agtgcgatgc cggtcagccg
     3661 gcgatcgtag tgcgtgaacc agttgttgag cacgaagttg ctggacatcg cgatgccggt
     3721 agcgatcacc gtcgcgatca cgaaatcggc ggtgacgacg atatgcagca accatacgaa
     3781 caacagttgc accaacacgc cggttgcccc cac
//
LOCUS       DATCZV010000356         3081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_81610, whole genome shotgun sequence.
ACCESSION   DATCZV010000356 DATCZV010000000
VERSION     DATCZV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81610"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VIW27_08050"
     CDS             <1..594
                     /locus_tag="VIW27_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY5739663.1"
                     /translation="VVDSGAIISGGNFHAEPVAFGADQCALAIAEIGSIAERRIATTV
                     DPAQNYGLPAFLTPEPGLNSGFMIAEDTAAALMAENKQRAMPCSIDSTPTSANQEDHV
                     SMACHAARRLGEMNRNLAHILAIELMIAVQGVEFRAPIETAPRLRRVMREIRERVPRL
                     ENDRYLADDIAAVRQLIDERRITAALGGDSPLPRLLP"
     gene            591..1373
                     /gene="hutG"
                     /locus_tag="VIW27_08055"
     CDS             591..1373
                     /gene="hutG"
                     /locus_tag="VIW27_08055"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEY5739664.1"
                     /translation="MKPRIVTGQGPLLLAQPHGGVEIPAEILARLNPVGREMADTDWH
                     IGRLYDGLAIDATIVSTPVHRYVIDANRDPADRSLYPGQNTTSLCPTTTFDGSEIYLP
                     GQAPSADEIEHRRQRYHRPYHDALAEQLARIQQRHGYAILYDCHSIRSRIPFLFDGGL
                     PDFNIGTNDGTSCAPSVESAIRGVCEQAGDYSWVANGRFKGGWTTRHYGDPERGCHAI
                     QMELAQCNYMRESSPWNYDAARADKLRAILAAILQNLTRVFD"
     gene            1388..1996
                     /locus_tag="VIW27_08060"
     CDS             1388..1996
                     /locus_tag="VIW27_08060"
                     /inference="COORDINATES: protein motif:HMM:NF022168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrbL family protein"
                     /protein_id="HEY5739665.1"
                     /translation="MLEITHKPIGMGKQRMCFVHPEDPRLAVKIPIAGDSSQNQREVR
                     FYRKLEKRGGVDCRHIPGFHGLCDTNRGPGIVVDLVRDYDGQISRPLTWYLAQGYPIE
                     EFEPFLEELKQSFLKNLIAFDQDMQVDKILVQKTSASKFRMVAIDGLGDSATAGLDIF
                     PFLVRHKIRRRWQGFMEHVYHCREVRLQREAQARAATAEQRA"
     gene            2025..2897
                     /locus_tag="VIW27_08065"
     CDS             2025..2897
                     /locus_tag="VIW27_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739666.1"
                     /translation="MGWLVLVAALGAVILFGGSAVATKIAVSAIDAVDVAIMRTVIGG
                     LLALPLAVMLRIPLPTTAAQRWLLLLSGFCGFIGFPLLFTLGVNLTSANHATMILAIL
                     PVLTGAIAMAWDRQPPAGPWWAGCAIAFSGEIMLIYDSAAAAANASVAGDLLVCLSTG
                     FASLGYVAGARLQRSGYTSRGTTFWGVCLFALLLLPAVPLVVDFDGLATAGAYAWSGL
                     LYQAVGITIIAYILWYWALGTGGIARVGLLQFLQPLSGVLLAYLLLSEGLSLIFLLSS
                     AMIMTGVMVAFRAR"
BASE COUNT          531 a         1044 c          958 g          548 t
ORIGIN      
        1 gtggtcgaca gcggagcgat catttccggc ggtaattttc atgccgagcc ggtagccttc
       61 ggcgccgacc agtgcgcgct ggcgatcgcc gagatcggct cgatcgccga gcgccgcatc
      121 gccaccacgg tcgacccggc gcagaattac ggcttgccgg ccttcctgac cccggagccg
      181 ggactgaact cgggcttcat gatcgccgag gacacggcgg ccgcgctgat ggccgagaac
      241 aagcagcgcg cgatgccctg ctcgatcgat tcgacgccaa ccagtgccaa ccaggaagac
      301 catgtctcga tggcctgtca cgcggcgcgg cggctcggcg agatgaatcg taacctcgcg
      361 catattctcg ccatcgagct gatgatcgcg gtgcagggcg tcgagttccg cgcgccgatc
      421 gagaccgctc cgcggctgcg gcgggtaatg cgggagattc gcgagcgcgt gccgcgactc
      481 gaaaatgatc gttacctggc cgacgacatt gccgcggtca ggcagctcat cgacgagcgg
      541 cgaattaccg cggcgctcgg cggcgatagc ccactgccgc ggctgctgcc gtgaagccgc
      601 gcatcgtcac cggccaggga ccgctgctgc tcgcgcaacc gcacggcggc gtcgagatcc
      661 ccgccgagat cctcgcgcgt ctcaatccgg tgggccgtga aatggcggat accgactggc
      721 acatcggccg cctctacgac ggcctggcca tcgacgcgac catcgtcagc acgcccgtgc
      781 atcgctatgt catcgatgcc aaccgcgacc cggcggaccg ctcactctat ccgggacaga
      841 acaccaccag cctgtgcccg acgacaacct tcgacggcag cgagatctat ctgccgggcc
      901 aggcgccgtc agcggacgaa atcgagcacc gccggcaacg ctaccaccgc ccttaccacg
      961 acgcgctcgc cgaacagctc gctcgcattc agcagcgcca cggttacgcg attctctacg
     1021 attgccactc gattcgatcg cggatcccat tcctgttcga cggcggcctg cccgacttca
     1081 atatcggcac caacgacggc accagttgcg ccccgtctgt cgagtcggcg atacgcggcg
     1141 tctgcgagca ggccggcgac tacagctggg ttgcgaacgg ccgcttcaag ggcggctgga
     1201 ccacgcgcca ctacggcgac cccgaacgcg gctgccacgc gattcagatg gaactcgcgc
     1261 aatgcaacta catgcgcgag agctcaccgt ggaattacga tgcggcgcgc gccgacaaac
     1321 tgcgtgctat tctcgccgcg atcctgcaaa atctgacgcg cgtcttcgac tagaccgcga
     1381 ttcgaatatg cttgaaatca cgcacaaacc catcggcatg ggcaagcaac gcatgtgctt
     1441 cgtacatccc gaggatcccc gcctggcggt caagattccc atcgccggcg acagcagcca
     1501 aaaccagcgc gaagtccgct tttaccgcaa gctcgaaaaa cgtggcggcg tcgactgccg
     1561 gcatattccc ggtttccatg ggctctgcga caccaaccgc gggccgggaa tcgtcgtcga
     1621 cctggtgcgc gattatgacg ggcaaatctc gcggccgctg acctggtacc tggcgcaggg
     1681 ttacccgatc gaggaattcg aacccttcct cgaggaactc aagcaatcgt tcctgaaaaa
     1741 cctgatcgcc ttcgaccagg atatgcaggt cgacaaaatc ctcgtacaga agacttcggc
     1801 gagcaagttc cgcatggtcg ccatcgacgg cctcggcgac tcggcaacgg ccggcctcga
     1861 cattttcccg ttcctggtaa ggcacaagat acggcgccgc tggcaaggct tcatggaaca
     1921 cgtctaccat tgccgcgagg tgcgcctgca gcgcgaagct caggccaggg cagcgacagc
     1981 cgagcaacgg gcctgaaccc ggccggccga tgacgggctg ctgaatgggc tggctcgtac
     2041 tcgtcgccgc gctcggcgcg gtgatcctgt tcggcggttc ggcggtagcc accaagatcg
     2101 cggtttcggc gatcgacgcg gtcgacgttg ccatcatgcg cacggtcatc ggcggactgc
     2161 tcgcactacc gctggcggtg atgttgcgaa ttcctctacc gacgacggca gcacagcgat
     2221 ggctgctgct gttatcggga ttctgcggct tcatcggttt tccgctgctg ttcacgctcg
     2281 gcgtcaacct gacctcggcc aaccatgcga cgatgatcct cgcgatcctg ccggtattga
     2341 ccggcgcgat cgcgatggcc tgggaccgcc agccgccggc aggcccgtgg tgggcaggct
     2401 gcgcgatcgc gttcagcggt gagatcatgc tgatctacga ttccgcggcc gcggcggcga
     2461 acgccagcgt cgccggcgac ctgctggtct gcctgtcgac cggattcgcc tcgctcggat
     2521 atgtcgccgg cgcgcgcctg cagcgcagcg gctatacgtc ccgcggcacc accttctggg
     2581 gtgtctgcct gttcgcgctg ctgctgctgc cggcggtacc gctggtcgtc gatttcgacg
     2641 gcctggcgac ggccggcgcc tacgcctggt cgggcctgct ttaccaggcc gtcggcatca
     2701 ccatcatcgc ctatatcctg tggtactggg cgctcggcac cggcggtatc gcacgagtcg
     2761 gattgttgca gtttctgcaa ccgctgtcgg gcgtgctgct ggcttacctg ttgctgagcg
     2821 aaggcctgtc gctgatcttc ctgctgtcct ccgcgatgat catgaccggc gtcatggtgg
     2881 cgtttcgcgc ccgctaggca acggttagac tgccgcagca tgacagtgcg cgaacccatc
     2941 cagctgccgg cgtgtgcggc gttgacggcg gcgcctgccg tgaccggatc gacgtcgctg
     3001 tccccgatta ccgacagcct ctatactgcg gttgccaacc gcgatcgtgc gtggccgcgg
     3061 tgatcgtcag cgtgcacaaa t
//
LOCUS       DATCZV010000357         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_241200, whole genome shotgun sequence.
ACCESSION   DATCZV010000357 DATCZV010000000
VERSION     DATCZV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241200"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VIW27_08070"
     CDS             complement(<1..737)
                     /locus_tag="VIW27_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5739667.1"
                     /translation="MMRFSDSQKLFAGGTAPLSGLGNLLQRLYLDGPLLGGLLLICAL
                     GLIVLYSAVSQNMDLWLQQIIRLFVALAAMLIVAQLPPDLMRRWTPWGYLAGLVLLVL
                     VLVTGEVGQGAQRWLNIGIRFQPSEIMKLAVPMMAAWYLHDRQLPPRFMDLVVIGVLI
                     IVPTVLIARQPDLGTSLLIAMSGVLVIVLAGLPIRIMILLAVASVPGAYVLWQFMQEY
                     QKQRVLTLLNPEADPLGAGYNIIQSKIA"
     gene            complement(724..>2564)
                     /gene="mrdA"
                     /locus_tag="VIW27_08075"
     CDS             complement(724..>2564)
                     /gene="mrdA"
                     /locus_tag="VIW27_08075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5739668.1"
                     /translation="RVILASAISIILLGIVIARLIQLQVIDHEQFAEQSQGNRIRIEA
                     VPPTRGLIFDRNGNVLAENLPAYQLELIPEQVPDIEDTLNRLARIGLIKKEDIPRFNG
                     LIQSGPRFKPVALRFRLSDDEIANFAVQRPRFPGVDLQPRLVRHYPDGELVAHAIGYV
                     GALSTADLERLDEASYAGTSHTGKTGVEHYNEDMLHGDVGFQQVVTNALGRQIPVETQ
                     EYADTMPERQRPVPGSNLHLSLDLDLQRVAYEQMAGRRGAIVAIDPNNGEILALVSSP
                     SFNPNAFATGMSSSEYNTLQTDEDRPLFNRAVRGSYPPGSTIKPMLSLAALEVGATNL
                     TRRSLCFGWYSLPGSTHRYRDWRPEGHGEVDLHDAIEQSCDVYFYEISRDIGIDNMHR
                     YLWQFGLGRNTGLDMVGEADGLVPSREWKRQAFRERADQVWFPGETIIASIGQGYMLA
                     TPLQLANATAALAVRGQRYRPHMVTAFEDPLNGRRTLIAPEPLARVEIDDPFYWDNIL
                     TAMHDVMQSPTGTARAAGQGAPYDMAGKSGTAQVFTVAQDEEYDEEEIEERLRDHALF
                     IAFAPLDAPEIAVSVIVENGSSGSRVAAPIARAVMDQYLGYADDAL"
BASE COUNT          491 a          789 c          813 g          471 t
ORIGIN      
        1 gcgatcttcg actggatgat gttgtagccg gcgccgagcg ggtccgcctc cggattcagc
       61 aaagtgagca cccgttgctt ctggtattcc tgcatgaact gccacaacac ataggcgcct
      121 ggtaccgacg cgacggcgag cagaatcatg atacggatcg gtagcccggc cagcacgatc
      181 acgagcacgc cggacatcgc gatcagcagc gaggtgccga ggtcgggctg acgagcgatc
      241 aataccgtcg gtacgatgat cagcacgccg ataacgacga gatccatgaa tctcggcggc
      301 agctgccggt catggaggta ccacgcagcc atcatcggca cagcgagctt catgatttcc
      361 gacggctgga agcgaatgcc gatgttgagc cagcgctgcg cgccctggcc gacttcgccg
      421 gtcacgagga caagcaccag cagcacgagt cccgcgagat aaccccacgg cgtccagcgg
      481 cgcatcaggt cgggcggcag ctgggccacg atcagcattg cggccagcgc aacaaacagg
      541 cggataattt gctgcagcca aagatccatg ttctgactca ccgccgagta gagaacgatc
      601 aggccgaggg cgcatatgag caacaatccg ccgagcagcg gaccgtccag atagaggcgc
      661 tgcagcaggt tacccaagcc gctcagcggc gcagtgccac ccgcgaacag tttttgcgaa
      721 tcgctaaagc gcatcatcag cgtagcccag gtattgatcc atcacggcgc gcgcgatcgg
      781 ggcagcaacg cggctgccgc tcgagccgtt ctccacgatg accgacaccg cgatctccgg
      841 cgcgtcgagc ggcgcgaagg cgataaacag ggcgtgatcg cgcagtcgct cctcgatctc
      901 ttcttcgtcg tattcctcat cctgagcaac cgtgaagacc tgcgccgtgc cgctcttccc
      961 ggccatatcg tagggcgcac cctgtcccgc ggcgcgtgcc gtgccggtcg gactctgcat
     1021 gacgtcatgc atcgccgtca ggatgttgtc ccagtagaac gggtcgtcga tctcgacccg
     1081 tgccaggggt tcgggtgcga tcagcgtgcg cctgccgttc agcggatctt cgaacgcggt
     1141 gaccatgtgt ggccggtagc gctggccgcg aactgccaat gccgccgtcg cattggcaag
     1201 ctggagcggc gtcgcgagca tgtagccctg accgatcgag gcgatgatgg tctcgccggg
     1261 aaaccacacc tggtctgcgc gctcgcggaa tgcctggcgt ttccactccc gtgacggaac
     1321 caggccatcg gcctcgccga ccatgtccag cccggtgttc cggccgagac cgaactgcca
     1381 caggtagcga tgcatgttat cgataccgat gtcgcggctg atctcgtaga agtagacatc
     1441 gcaagactgc tcgatcgcgt cgtgcaggtc gacctcgcca tgcccttcag ggcgccagtc
     1501 gcgatagcgg tgggtgctgc ccggaaggct gtaccaaccg aagcaaagcg accgccgggt
     1561 gagattcgtg gccccaacct ccaacgcggc cagcgacaac atcggcttga tcgttgaacc
     1621 gggcggatag ctgccgcgaa cggcgcgatt gaacagcggc cggtcttcgt ccgtctgtaa
     1681 agtgttgtat tcgctcgagc tcatgcccgt ggcgaaagca tttggattga aggagggcga
     1741 actcacgagt gccaggatct cgccgttatt gggatcgatc gccacgatcg caccccggcg
     1801 gccggccatt tgttcgtagg ctacgcgctg caagtcgaga tccaggctca ggtgcaggtt
     1861 cgagcccggc acggggcgct gccgctcggg catcgtgtcc gcgtattcct gggtttcgac
     1921 cgggatctgg cgaccgaggg cgttggtgac cacctgctgg aagccgacat caccgtgcag
     1981 catgtcttcg ttgtagtgct ctacgccggt ttttcccgta tgcgatgtgc ccgcgtatga
     2041 ggcctcgtcg agtcgctcga ggtcagcggt gctcagtgcg ccaacgtagc cgatcgcgtg
     2101 ggccacaagc tcgccgtccg ggtaatggcg aacgaggcgc ggctgcagat cgacacccgg
     2161 gaacctcggc cgctggacgg caaaattcgc gatctcgtca tcgctcagtc gaaagcgcag
     2221 cgccaccggc ttgaaccgcg gaccgctttg gatgagtccg ttgaatcgcg ggatatcttc
     2281 ttttttgata agcccgatgc gggcgagccg attgagggtg tcctcaatat ccggcacctg
     2341 ctccgggatc agctcgagct ggtaggctgg caggttctcg gcaagcacat tgccgttgcg
     2401 atcgaagatc aggccgcgcg tcggcggtac ggcttcgatg cgaatgcgat taccctggga
     2461 ttgttctgca aattgctcat gatcgatgac ctgcagctga atcagcctgg caatgacgat
     2521 tcccagaagg atgatgctga tggcggaggc gagtatcacc cggg
//
LOCUS       DATCZV010000358         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_347155, whole genome shotgun sequence.
ACCESSION   DATCZV010000358 DATCZV010000000
VERSION     DATCZV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347155"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /gene="tadA"
                     /locus_tag="VIW27_08080"
     CDS             complement(<1..404)
                     /gene="tadA"
                     /locus_tag="VIW27_08080"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY5739669.1"
                     /translation="MRRALEQARKAAALGEVPVGAVLVGESGELIAEGHNQPIAAHDP
                     SAHAEIVVLRAAAQQLGNYRLPGTTLYVTIEPCVMCVGALVHARVQRLVYGAVEPRTG
                     AIASAHRLFDSGEFNHRPRIDAGILADECARL"
     gene            complement(419..1993)
                     /gene="guaA"
                     /locus_tag="VIW27_08085"
     CDS             complement(419..1993)
                     /gene="guaA"
                     /locus_tag="VIW27_08085"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5739670.1"
                     /translation="MPDIHDDKILILDFGSQYTQLIARRVREAGVYSEIYPWDADFEQ
                     IRAFGARGFILSGGPESTLDAQKPEIPPALFDLGVPILGICYGMQAIAQQLGGKVENV
                     GKAEFGYASVRARGHTELLRDIQDHQTAEGYGMLDVWMSHGDRVAELPPGFKLMASTE
                     SAPIAGMADEQRRIYGLQFHPEVTHTLQGKAIYARFLHQICGCGCEWTPANIIDATIA
                     AVREQVGADQVVLGLSGGVDSSVVAALLHEAIGEQLTCIFVDTGLLRYREGDMVMDTF
                     AEHMGVRVIRVDAEVRFLNKLEGLADPEDKRKAIGNLFIEIFEEQAGKLANAKWLAQG
                     TIYPDVIESAGAATGKAHLIKSHHNVGGLPEEMKLALVEPLRELFKDEVRLIGVELGL
                     PREMVYRHPFPGPGLGVRILGEVKKEYADTLRLADHIFIDELRKHDLYDKVSQAFVVF
                     LPVKSVGVTGDGRRYNHVVSLRAVETIDFMTARWADLPYHFLDHVSRRIMNEIEGIAR
                     VVYDISGKPPGTIEWE"
     gene            complement(2116..>2433)
                     /locus_tag="VIW27_08090"
     CDS             complement(2116..>2433)
                     /locus_tag="VIW27_08090"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5739671.1"
                     /translation="YKSYRGMGSIAAMQKGSSDRYFQAENADEKLVPEGVEGRVPFKG
                     PLTNTIYQLLGGVRSSMGYVGCATIDEMRTRPEFVKVTNAGMRESHVHDVSITKEAPN
                     YQV"
BASE COUNT          438 a          794 c          755 g          446 t
ORIGIN      
        1 atcagccgcg cgcattcgtc ggccagtatg cccgcatcga ttcgtggacg gtggttgaac
       61 tcgccgctgt cgaagagccg gtgagcactg gcgatggcgc cggtacgggg ctcgactgcg
      121 ccgtacacca gccgttgcac acgcgcatgc accagtgcgc cgacacacat cacgcagggc
      181 tcgatggtca cgtagagcgt ggtgccgggc agacggtaat tgcccagctg ctgcgccgcg
      241 gcgcgcaaca cgacgatctc ggcgtgcgcg ctcgggtcgt gcgcggcgat cggctggttg
      301 tggccctcgg caatcaattc gccgctttca ccgaccagca ccgcacccac cggcacttcg
      361 cccagcgcgg ccgctttgcg ggcctgctcg agcgcccgcc gcatccagtc ctcgtcgctt
      421 actcccactc gatggtgccc ggcggcttgc cggagatgtc gtagacgacg cgcgcgatgc
      481 cctcgatctc gttcatgatg cggcgcgaca cgtgatcgag aaaatggtac ggcaaatcgg
      541 cccagcgcgc ggtcatgaag tcgatggttt cgaccgcgcg caggctgacc acatgattgt
      601 aacgacgccc gtcaccggtg acgccgaccg acttgaccgg caggaagacg acgaaagcct
      661 gcgacacctt gtcgtagaga tcgtgcttgc gcagctcgtc gataaagatg tgatcggcga
      721 gacgcagcgt atcggcatat tcctttttga cttccccgag aatacgcacg ccgagacccg
      781 gacccgggaa cgggtggcga taaaccattt cgcgcggcag gccgagctcg acgccgatca
      841 ggcgtacctc gtccttgaac agttcgcgca gcggttcgac cagcgcgagc ttcatttcct
      901 ccggcaggcc gccgacgttg tggtgcgatt ttatcaggtg cgccttgccg gtagcggcac
      961 cggcggattc gatcacgtcg gggtagatcg taccctgcgc gagccatttg gcgttggcga
     1021 gcttgccggc ctgttcctcg aagatctcga tgaacaggtt accgatcgcc ttgcgcttgt
     1081 cctcggggtc ggcaagcccc tcgagcttgt tcaggaaacg tacctcggca tcgacgcgaa
     1141 tcacacgtac ccccatgtgc tcggcgaagg tatccatgac catgtcgccc tcgcggtagc
     1201 gcagcagacc ggtgtcgacg aagatgcagg tcagctgctc gccgatcgcc tcgtgcagca
     1261 gcgcggcgac caccgacgaa tcgacgccgc cggacagtcc gagcaccacc tggtcggcac
     1321 cgacttgctc gcgcaccgcc gcgatcgtcg cgtcgatgat gttcgccggg gtccactcgc
     1381 aaccgcagcc gcagatctgg tgcaggaagc gcgcataaat cgccttgccc tgcagcgtgt
     1441 gcgtgacctc cggatgaaac tgcagcccgt agatgcgccg ctgctcgtcg gccataccgg
     1501 cgatcggcgc actttcggtg ctcgccatca gcttgaagcc gggcggcagc tcggcgacgc
     1561 ggtcgccgtg cgacatccac acgtcgagca taccgtaacc ctcggcggtt tggtggtcct
     1621 ggatgtcccg caacaactcg gtgtggccgc gcgcacgcac gctggcatag ccgaactcgg
     1681 ccttgccgac gttctcgacc ttaccgccga gctgctgcgc gatcgcctgc ataccgtagc
     1741 agatgccgag tatcggtact ccgagatcga acaaagcggg cggaatctcc ggcttttgcg
     1801 cgtcgagcgt ggactcgggg ccgccggaga gaataaaacc acgcgcgccg aaagcgcgaa
     1861 tctgctcgaa atcggcatcc caggggtaaa tctccgagta gaccccggcc tcgcgcacgc
     1921 ggcgcgcgat cagctgcgtg tactgcgagc cgaaatcgag aatcaggatt ttgtcgtcgt
     1981 ggatatcggg caaggaatca gacctctcgg cattgagtca tccccgcgca agcgcggatc
     2041 ttgtagttaa cggaatgaaa atcggcacgc gaaccccgct tgcgcgggga cgacgatgct
     2101 ggttcgaact cgccatcaaa cctggtagtt cggcgcttcc ttggtgatcg agacatcgtg
     2161 cacgtgcgac tcgcgcatac cggcgttggt caccttgacg aattccgggc gggtacgcat
     2221 ttcgtcgatg gtcgcacagc cgacgtagcc catgctcgaa cgcacgccgc cgagcagctg
     2281 gtagatggtg ttggtcagcg gccccttgaa cggcacccgc ccctcgacgc cttccggcac
     2341 cagtttttca tcggcattct cggcctggaa ataacgatcg ctggacccct tttgcatcgc
     2401 cgcgatcgag cccatgccgc ggtaggattt gta
//
LOCUS       DATCZV010000359         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_717622, whole genome shotgun sequence.
ACCESSION   DATCZV010000359 DATCZV010000000
VERSION     DATCZV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717622"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /gene="plsB"
                     /locus_tag="VIW27_08095"
     CDS             complement(<1..1995)
                     /gene="plsB"
                     /locus_tag="VIW27_08095"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HEY5739672.1"
                     /translation="MKKQINRLLRWILFLWVRVEVFPEDVSSLGMNPQRPILYVLADR
                     GLADLLVLGEIARRRHLPDPLARLPLAGLDRHHSIYSVASRSPMIDWFKRRRKRSEML
                     ADILRAFRDQRELELQIVPVSVFWGRPLARQKSWLQVLFADTWSLAGRTRKFFTILFQ
                     GRNTRLIFSQPIELRALIDSDNAGEPELQQLLTATLNQQREATFGPQIISHKQLSDRV
                     LEDPLVAELTSETADSTFLSRTRARRYCREIFADCTQLTIELMLRLLRTFWNRFYSGI
                     EVYNIERVREAALTRQLVYVPCHRSHVDYLLLSYVIYIENLALPYIAAGDNLNIPFIG
                     RILRGGGAFFIRRSFKDNHLYTAVMRAYLYQLVKLGVPLEYFVEGGRSRTGRMLKPKI
                     GMLGMTVEAFIKNQSKPMAFVPVYLGYEKLIEGKAYLGELYGGKKQRESTLGALGAIF
                     RLKGHFGRVTASFGEPIEIETLLERHCGNWRERGEELEQRPNWFRDSLLDLGTEIMYG
                     INRACVINPVNSIATILLATPRQSIEIEELITQAALHARVVEGISCLDSIRVAGEVDR
                     ERVEAIARQKVIRLRRHEYGDIVYLEPRDTVLISYYRNNSLHAIVIPALVACCFVNLR
                     RVSRDSVQRRIALLFPFLKSELQLEWQDASLAEIIDQSLDS"
BASE COUNT          435 a          618 c          691 g          413 t
ORIGIN      
        1 gctgtcgagc gactggtcga tgatctcggc caggctggcg tcctgccatt cgagctgtag
       61 ctcggacttg agaaacggga acagcagcgc gatgcggcgc tgtacgctgt cgcgggatac
      121 gcgcctcagg ttgacgaagc agcaggcgac cagcgccggt atcacgatcg cgtgcaggct
      181 gttgttgcgg tagtagctga tcagcaccgt gtcacgcggc tcgaggtaga cgatgtcgcc
      241 atactcgtgg cgccgcagcc gaattacctt ttgtcgcgca atggcctcga cgcgctcgcg
      301 gtcgacctcg ccggcaacgc gaatcgaatc gaggcaggaa atgccctcga ccacccgggc
      361 gtgcagtgcg gcctgggtga tcaattcctc gatttcgatg ctttgccgcg gcgtcgccag
      421 caggatcgtg gcaatcgaat tgaccgggtt gatcacgcag gcgcgattga tgccgtacat
      481 gatctcggtg ccgagatcga gcaggctgtc gcgaaaccag ttcgggcgct gttcgagttc
      541 ttcaccgcgc tcgcgccagt tgccacagtg ccgctcgagc agggtctcga tctcgatggg
      601 ttcgccgaaa ctcgcggtta ccctgccgaa gtgtcccttg agccgaaaga tcgcgcccag
      661 cgcaccgaga gtcgattcgc gctgcttctt gccgccgtag agttcgccca ggtaagcctt
      721 gccctcgatc agcttttcat agccgaggta taccggcacg aaggccatcg gcttcgactg
      781 gttcttgatg aaggcctcga cggtcatgcc gagcataccg atcttcggct tcagcatgcg
      841 tccggtacgg ctgcggccgc cctcgacgaa gtattccagc ggcacgccga gcttgaccag
      901 ctggtacagg taggcgcgca tgaccgcggt gtagagatgg ttgtccttga agctgcggcg
      961 aatgaaaaag gcgccgccgc cacgcagaat gcgaccgata aacggaatgt tcagattatc
     1021 gccggcggcg atatagggca gggcgaggtt ttcgatgtag attacgtagg acagcagcag
     1081 gtaatcgacg tggctgcggt ggcagggcac gtaaaccagc tggcgggtca gggcggcctc
     1141 gcgaacgcgc tcgatattgt agacctcgat gcccgagtaa aaacggttcc agaatgtccg
     1201 cagcaggcgc agcatgagct cgatcgtcaa ctgcgtgcag tcggcaaata tttcacggca
     1261 atagcggcgg gcacgtgtac ggctgaggaa agtgctgtcg gcggtctccg aggtcagctc
     1321 ggcgaccaga ggatcctcca gcacccggtc ggagagctgc ttgtggctga tgatctgcgg
     1381 gccgaaggtc gcttcccgct gctggtttag cgtcgccgtc agtagttgct gcagctcggg
     1441 ttcaccggcg ttgtcgctat cgatcaatgc gcgcaactcg atcggctgcg aaaaaatcag
     1501 tcgcgtattg cgaccctgga agaggatggt aaaaaatttc cgagtccgcc cggccagcga
     1561 ccaggtatcg gcgaacagca cctgcagcca gcttttctgg cgcgccagcg gccggcccca
     1621 gaacaccgat accggtacga tctgcaattc gagttcgcgc tggtcgcgaa aggcgcgcaa
     1681 aatgtcggcg agcatttccg aacgcttgcg ccggcgcttg aaccagtcga tcatcgggct
     1741 gcgcgaggcc acgctataga tggaatgatg cctgtcaagg ccggccagcg gcagccgcgc
     1801 gagcgggtcg ggcaggtgac ggcgcctggc gatttctccg agaaccagca agtcggcgag
     1861 cccgcggtcg gcgagcacgt agagtatcgg gcgctgcgga ttcatgccga ggctggacac
     1921 atcttcggga aaaacttcta ctctaaccca gagaaacagt atccagcgga gtaatcgatt
     1981 gatctgcttt ttcaacagga acctgcggat ttgcgaccag tctgacgata ataacccctc
     2041 gaggacgaac atatgaacaa tatcaggatg gaattcgata acgggcgcgg cgaaacgctg
     2101 gccggattac tcgaattgcc ggacggcggc ggcagtgtct cgagctatgc gctgttc
//
LOCUS       DATCZV010000360         3569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_293727, whole genome shotgun sequence.
ACCESSION   DATCZV010000360 DATCZV010000000
VERSION     DATCZV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293727"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..890)
                     /locus_tag="VIW27_08100"
     CDS             complement(3..890)
                     /locus_tag="VIW27_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739673.1"
                     /translation="MEFNNLKAAFLVVIAMTMITTNDAIVKHITQVFNVGQIMFLRGT
                     LICVIFALVMRLRNQPVFGPQAFHRWNLARALFELGATLSFLTGLSLLPIAVASTLGF
                     ASPIFLALLAAVLLKENVGALRWSIIAAGFGGVLLITNPFADSTSWAVIFPLICAFFV
                     ALRDLAIRYVPATIPSSQVAFTNAWIVALGGGLLSIFQGWGSADWHWYLWFFVLAGAL
                     YSGYLLLIVGSRIGELSFIGPFKYISILIAIGYGYWIWGDRPSANMLAGALVIVVSGI
                     ILVSSEKRKVRQAALATRF"
     gene            1174..2487
                     /locus_tag="VIW27_08105"
     CDS             1174..2487
                     /locus_tag="VIW27_08105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5739674.1"
                     /translation="MQIWTRALKFAAQTPPERNRYVDFLRAISILVVIFGHWLITTAY
                     YTDNALSVGHLFEAQPWTQWLTWMFQVMPVFFIVGGYSNAVSLDSARRRQLTYADWLA
                     GRLHRLVIPLLLVLLFWMVLTAGLDALGIERSILQLGSQVALIPTWFLAIYIMVVVLA
                     PLMYRCWRRLGFVAFWLLVALAVAMDLVFFAVDLRWPAWSNYFWVWLAIHMLGFAWRD
                     ARFGGAGRLLLYSMLGVATLWVLVRFGPYPLAMVGSPGQTLSNNTPPKITLLALAVFQ
                     FGLLLALERPLRVMLDNLYLWAATVLINGMIMSIYLWHATVSVIFIGLLYLGGGVGLG
                     LEPGSAAWWWSRLPWLLLLAGLLVPVAMLVSPLERIARDPAAAACHPVRQVIGAMMTG
                     LGIAMLAMFGYGGGPVAWLGFLSFTLVVGGAAVGGLLPGLRLQRD"
     gene            complement(2628..3401)
                     /locus_tag="VIW27_08110"
     CDS             complement(2628..3401)
                     /locus_tag="VIW27_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5739675.1"
                     /translation="MAAVDVSYRVSGSGPPLYLVHGIGARNTTWDAVIAGLEDAFSCV
                     SYNLRGHGDSPVPPTPYSLHDLVEDLEALRRRLGHQCIHIAGHSLGGMIGPAYARAYP
                     RHTASVSLLSTAAGRSDEDRAKLAAVGTAMRQRGIAETLPTFVSRWFTDDFIAAHPRR
                     VEARLRQVTDTPAEVFLSVFDIYATTEMAPWLHEVECPCLVLTGEFDGGCNPRLNEFI
                     HSRLPNSWLVILDGLKHALLIEASDRVLPRLREFLLDNS"
BASE COUNT          607 a         1087 c         1186 g          689 t
ORIGIN      
        1 gctcagaagc gggttgccag ggcggcctgc ctgactttgc gtttttcgct ggataccaag
       61 atgatgcccg agacgacgat aacaagggct ccggcgagca tattggcgct cggccggtcg
      121 ccccagatcc agtagccgta gccgatcgcg atcaggatgc tgatgtactt gaacggtccg
      181 atgaaggaca gctcgccgat gcggctgcct acgatgagca gcaggtagcc gctgtagagc
      241 gcgccggcca atacgaaaaa ccacaggtac cagtgccagt cggcgcttcc ccagccctgg
      301 aagatagaca gcagcccgcc gccgagggcg acgatccagg cattggtgaa ggcgacctgg
      361 ctcgacggaa tagttgccgg cacatagcga atcgcgagat cgcgcagcgc aacgaagaat
      421 gcgcagatca acgggaaaat gaccgcccag gatgtcgaat ccgcaaacgg gttggtgatc
      481 agcagcacgc cgccgaagcc ggcagcgatg atcgaccagc gcagcgcacc gacattttcc
      541 ttcagcagca cggcggcgag cagcgcgaga aaaatcggcg atgcgaagcc cagcgtcgag
      601 gccaccgcga tcggcagcag cgacaggccg gtcaggaagc tgagcgtcgc gccgagttcg
      661 aacagcgcgc gcgccaggtt ccagcggtga aaggcttgcg gcccgaacac cggctgattg
      721 cgcaggcgca tgaccagcgc gaagatcacg cagatcagcg tgccgcgcag aaacatgatc
      781 tgaccgacgt tgaatacctg cgtgatatgt ttgacgatgg cgtcgttggt ggtgatcatc
      841 gtcatcgcga tgaccaccaa aaaggccgct ttcaggttgt tgaattccac gccgcgcagc
      901 gtagcagcag cctcgatcgg gttcgagaat tatttatcgt cacttgccag gggcggacgc
      961 cggctgttga ccgacgcctg gcaacggggg ggagtctgct cgcccgcggg cgtcgcgccc
     1021 ggcgtccgtt tgcgccaaag cagcgtaatc caggccgacc gccgtttggc ttgcttcggc
     1081 ctgggctttt atgcccgatg cggctgaccg ggatcagtat tctggccgcg ctggagtata
     1141 aaataacaac gcatcggcat tggtgactgc gcaatgcaaa tctggacccg ggccctgaaa
     1201 ttcgcggcac agacgccgcc cgagcgaaat cgctatgtcg acttcctgcg agcgatctcg
     1261 attctggtgg tgattttcgg gcactggcta atcaccaccg cctactacac cgataacgct
     1321 ctttcggtcg ggcacctgtt cgaggcgcag ccgtggacac agtggctgac ctggatgttc
     1381 caggtgatgc cggtgttttt catagtcggc ggatactcca atgcggtttc gctcgacagt
     1441 gcgaggcgca ggcagctcac ctatgccgac tggctggccg gccgcctgca tcggctggtt
     1501 attcctttgt tgctagtgct gcttttctgg atggttctga ccgcgggtct cgacgcgctc
     1561 ggtatcgagc gatcgatact tcagcttggc tcgcaggtcg ccctgattcc gacctggttt
     1621 ctggcgatct atatcatggt cgtggtgctc gccccgctca tgtatcgctg ttggcgccgc
     1681 ctgggtttcg tcgctttctg gctgctggtg gcgctggcgg tcgcgatgga cctggttttc
     1741 ttcgccgtcg atctgcgttg gcccgcctgg agtaattatt tctgggtctg gctggccatc
     1801 catatgctgg gcttcgcctg gcgcgacgcc cgatttggcg gtgccggcag actactgttg
     1861 tactcgatgc tcggtgtggc gactttgtgg gtgctggtgc gattcggtcc ctacccgctg
     1921 gcaatggtcg gctcacccgg acagacgctg tccaacaata ctccgccgaa gatcaccttg
     1981 ctggctctcg cggtatttca gttcggcctg ttgctcgcgc tcgagcgccc gctgcgcgtg
     2041 atgctcgata acctgtatct gtgggccgcc acggtgctga tcaacggcat gatcatgagc
     2101 atctacctgt ggcatgcgac agtcagtgtc atcttcatcg gtctgctcta cctcggcggc
     2161 ggtgtggggc tgggcctgga accgggtagt gccgcctggt ggtggtcgcg gcttccctgg
     2221 ctgctgctgc tggcgggctt gctggtgccg gtcgcgatgc tggtgtcacc gctggagcgt
     2281 atcgcccgcg acccagccgc cgccgcctgc cacccggtcc ggcaggtcat cggtgcgatg
     2341 atgacgggtc tgggcatcgc gatgctggcg atgttcggtt acggcggggg gcccgtggcg
     2401 tggctgggtt tcctgtcctt taccctggtg gtcggcggcg ccgctgtcgg cggcctgctg
     2461 ccgggcttgc gtttgcagcg cgactgatcg ccgccggcga tcgaccagcg acggcgagtc
     2521 aagccagcca ggagtcgcca cgcagaccga caaacggccg gcattccggt cgccgctagc
     2581 ggcgccgcaa ctggatccgg tcgacggcga cgcgtggttg ccgattgtca gctattgtcg
     2641 agcagaaact cgcgcaaccg cggcagcacg cgatcactgg cctcgatcag cagcgcatgc
     2701 ttgaggccgt cgagaatcac cagccacgaa ttcggcagcc gcgaatgaat aaactcgttg
     2761 aggcgcgggt tgcagccgcc gtcgaattcc ccggtcagca ccaggcacgg gcactcgact
     2821 tcgtgcagcc agggcgccat ctcggtggtc gcatagatat cgaagacgct caggaatacc
     2881 tcggccggcg tatcggtcac ctgccgcagg cgggcttcga cccggcgcgg gtgggcggcg
     2941 atgaagtcgt cggtaaacca gcgcgagacg aatgtgggga gggtttcggc gatgcctcgc
     3001 tgccgcatcg cggtgccgac ggcggcgagc ttcgctcgat cctcgtcgct gcgacccgcg
     3061 gcggtgctga gcaggctgac gctcgcggta tggcggggat aggcacgcgc ataggccggc
     3121 ccgatcatgc cgccgagcga atgaccggcg atgtgaatgc attggtggcc gagcctgcgg
     3181 cgcagggctt cgagatcttc gaccaggtcg tgcagcgagt agggggtcgg cggcacgggg
     3241 ctgtcgccgt ggccgcgcag gttgtagctg acgcagctga aggcgtcctc gaggcccgcg
     3301 atcacggcat cccaggtcgt gttgcgcgcg ccgataccat gcaccaggta aagcggcggt
     3361 ccgctgccgc ttacccggta actgacgtcg acggcggcca tcgcccgatc agccccagcg
     3421 tgccgcggca acgccggcga aatgcggcat cacttcctcg gcgaattgct gaatcgattc
     3481 cagcacctcg gcggtctcga cgccgaaatt gacattcatg atgaagcggt cgacaccggc
     3541 ctcggcatag ggagcgagcc tgtcgatca
//
LOCUS       DATCZV010000361         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_718107, whole genome shotgun sequence.
ACCESSION   DATCZV010000361 DATCZV010000000
VERSION     DATCZV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718107"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1051)
                     /locus_tag="VIW27_08115"
     CDS             complement(134..1051)
                     /locus_tag="VIW27_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5739676.1"
                     /translation="MNDQSLPTRDFIGYADRPPNVNWPGDARVAVNFCINYEEGGELC
                     VLNGDDRSEIRLSDVDVVARPGGRDLNIESSYEYGSRVGYWRLLKAFTDRGLTATVNL
                     VGLAGQRNPRALRAMIDAGFDLQPHGWRWFDYHELDVDTEREYIRRSIEQVRELTGEP
                     PLGYYAGLPSLNTRRLVVEAKCFVYDSDVYNDDLPYWSPDYPGLLLVPYSLDTNDSKF
                     GRAESDYQLGEEFFTYLKDSFDTLYAEGETQPKMMTVGLHARLVGRPGRIGSLHAFLD
                     YLQGHERVWICRRGDLARHWIEHHPNPGP"
     gene            1246..1851
                     /locus_tag="VIW27_08120"
     CDS             1246..1851
                     /locus_tag="VIW27_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019029100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY5739677.1"
                     /translation="MIDDIRRKLLLALAGLPVAGIAGAAVKTPPASEGPFYPTPAMRY
                     VDTDNDLVRIDGEVESAGGEIVRLGGRVLDADGNPVAGARVEIWQCDVTGRYLHRGDT
                     GNLRDRAFQGFGHDITAEDGVYRFRTIKPVPYGSRTPHIHVKVWVGGRERLTTQFYLP
                     DHPGNDRDWLYRRVPAELRETVTMRFAAGGNEPEASLDIVI"
     gene            complement(1859..2086)
                     /locus_tag="VIW27_08125"
     CDS             complement(1859..2086)
                     /locus_tag="VIW27_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739678.1"
                     /translation="MLEIIVDSMLLLLLASIALWVWIYRRLDREHPERGRYYRWHQLL
                     ILLIAALAILLGVIIVLSSDPGGPDLYLQGY"
     gene            complement(2104..>2362)
                     /locus_tag="VIW27_08130"
     CDS             complement(2104..>2362)
                     /locus_tag="VIW27_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005240055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5739679.1"
                     /translation="GRQKINLHQLGAEFEPHAGRVQAGSADLCFIIDEPLDEAAEELR
                     SRGVDIIDGPLARSGAEGALLSLYFRDPDGNLIEIANYASG"
BASE COUNT          429 a          734 c          773 g          426 t
ORIGIN      
        1 ggtcggaatt agtcatgcgc acgccctgtc aggataaaac cgggattgtc gcacaatagg
       61 cgggctgcgc aactcccccg tagtacaaag cggccacgcg attcgtgccg gctccggctg
      121 cgtaaccggg gattcagggt ccgggattgg ggtgatgctc gatccagtgg cgcgccaggt
      181 cgccgcggcg gcagatccag acgcgttcgt gaccctgcaa atagtctaga aaagcgtgca
      241 ggctgccgat tcgccccggg cggcccacca gccgagcatg caggcccacg gtcatcatct
      301 tcggctgggt ttcgccctcg gcatacaggg tgtcgaagct gtccttcagg taggtgaaaa
      361 actcttcgcc gagttgatag tcgctctccg cgcggccgaa cttggaatcg ttggtgtcga
      421 gcgagtaggg caccagcagc aggccgggat agtcgggcga ccagtagggc aggtcgtcgt
      481 tgtagacgtc ggaatcgtag acgaaacatt tggcctcgac caccagccgg cgggtgttca
      541 gcgacggcag gccggcatag tatccgagtg gcggctcacc ggtgagttcg cggacctgct
      601 cgatactgcg gcgaatatac tcgcgctcgg tatcgacatc cagttcgtgg tagtcgaacc
      661 agcgccagcc gtgcggctgc aggtcgaagc cggcgtcgat cattgcccgc agggcgcgcg
      721 gattgcgctg cccggcgagc ccgacgaggt tgacggtggc ggttaggccg cggtcggtaa
      781 aggctttcag cagccgccag taaccgactc tcgagccgta ttcgtagctg gattcgatat
      841 tcaggtcgcg gccgccgggg cgcgcgacca cgtcgacgtc cgacagcctg atctcggagc
      901 ggtcgtcgcc gttgagtacg cagagttcgc cgccctcctc gtaattgatg cagaagttca
      961 ccgcgacccg ggcatcgccg ggccagttga cgttcggcgg ccgatcggcg taaccgatga
     1021 aatcgcgcgt cggcagggat tgatcattca tgggcattcg gccagtcggt tgcaggttgc
     1081 gcgctgtact cgtcgcggtg aatcgaatct tatatcattg gcgcccggca tcaaggcaaa
     1141 cgggtatttg ccgcgcgcta gcgggccgtg aaccctggcg cctggcggcg gtcgaaacaa
     1201 agcatgcctt gcagcagcga accctgatcg acaggagata tttcgatgat tgatgatatt
     1261 cggcgcaaac tgttgctggc gctcgccggc ctgccggttg ccggcatcgc cggcgccgcg
     1321 gtgaaaacac cgcctgccag cgaggggccc ttctacccga ctccggcaat gcgctacgtc
     1381 gataccgaca acgacctggt gcgaatcgac ggcgaggtcg aaagcgccgg cggcgagatc
     1441 gtgcgtctgg gcggcagggt gctcgacgcc gatggcaatc cggttgccgg ggcccgggtc
     1501 gaaatctggc aatgcgacgt caccggccgt tacctgcatc gcggtgatac cggcaatctg
     1561 cgcgaccgcg cctttcaggg cttcggccac gatattaccg ctgaagatgg cgtttaccgc
     1621 tttcgcacca tcaagccggt gccttatggc agccgcacgc cgcacatcca cgtcaaggtc
     1681 tgggtcggcg gccgcgagcg cctgacgacg caattctacc tgcccgatca tcccggtaac
     1741 gatcgcgact ggctctaccg ccgtgttccc gccgaactgc gggagactgt gaccatgcgc
     1801 tttgccgccg ggggtaacga acccgaggcc agcctcgaca tcgtcatcta ggcgatagtc
     1861 aataaccctg caggtacagg tcgggcccgc cgggatcgct cgatagcacg ataatcacgc
     1921 cgagcaggat cgccagcgcg gcgatcagca ggatcagcag ctggtgccag cggtagtagc
     1981 ggccgcgctc cggatgttcg cgatcgaggc gtcgataaat ccagacccac agcgcgatgc
     2041 tcgccagcag caacaggagc atcgagtcga cgataatttc cagcatcagc cgctcccgcg
     2101 ccgctagccg ctggcgtagt tggcgatctc gatcaggttg ccgtcgggat cgcgaaagta
     2161 gagcgacagc aacgcgccct cggcgccgct acgcgccagc gggccgtcga tgatatcgac
     2221 gccccggctg cgcagttctt cggcggcctc gtcgagtggt tcgtcgatga tgaaacacag
     2281 gtctgcgctg ccagcctgca cgcgaccggc atgcggctcg aactcggcgc ctagctggtg
     2341 caggtttatt ttctgccggc cg
//
LOCUS       DATCZV010000362         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_82352, whole genome shotgun sequence.
ACCESSION   DATCZV010000362 DATCZV010000000
VERSION     DATCZV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82352"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VIW27_08135"
     CDS             <1..852
                     /locus_tag="VIW27_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY5739680.1"
                     /translation="SQAAVIIDAAPIDRTRRHAIAREIGMPATAFVEEIGADWIVAQF
                     MSTVMELPMCGHGTICLFTHLLESGLVSLDETDSCSFELRLPKSSARVELSRRGDNRI
                     QVMLDIRAPTFETAPPHTEALLTLLGLDSGALDTTLPLETARGDFIHLVVPIAGLAAM
                     RAIKPDFDGMVEFCNAHGIETIAAFCREVENPENTIHVRDFCPAVGVSESAAAGTTNA
                     ALTSYLLRHAMLPAGKSVVSLNAEQGLELGRPSSIRSIVTPGDGGIARLQVGGVATRV
                     FDGQVYL"
     gene            863..1852
                     /locus_tag="VIW27_08140"
     CDS             863..1852
                     /locus_tag="VIW27_08140"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY5739681.1"
                     /translation="MTTDLEQLYREALVWDAHAGVFPSPQVDLNLLDEWRQLGASYVS
                     INVGFDVMNWQETLATLAAYRRWVLRNTDHFVLANDAVDIDRARAAGKLALSFDIEGM
                     NALNEDLNMVGLYHALGVRQMLFAYNLNNAAAGGCHDRDTGLTAFGREVVQEMNRVGM
                     IVDCSHAGYRTTMDIMQVSQKPVVFSHSNPAAVWPHQRNIDDEQIRACAATGGVIGLN
                     GMGIFLGDNDISVDTMVRHVCYVCDLVGSEHIGIGFDYSPGIDVDIGVILRSRPDYWP
                     AGQQYDTRDIKHGGPAQLPALIARLADAGFDDVDLRGFLGENFRRVALEAWSG"
     gene            complement(1936..>2396)
                     /locus_tag="VIW27_08145"
     CDS             complement(1936..>2396)
                     /locus_tag="VIW27_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY5739682.1"
                     /translation="VGALLDSIPGLGSIALVLMLLYYVFAVIATKLFGANFPQWFGSI
                     GESLFSLFQIMTLESWSMGIVRPVMEVHPNARIFFVIFILIATFTMLNLFIAIIVNAM
                     HSFTEQESQERQEALDQTRDHIEADLHQEVASLRSEIAELKQLLQESPNR"
BASE COUNT          497 a          716 c          714 g          469 t
ORIGIN      
        1 agccaggccg cggtcattat cgatgcggcg cccatcgatc gcactcgacg ccacgcgatc
       61 gcgcgtgaaa tcggcatgcc ggcgaccgcc ttcgtcgaag agatcggtgc agactggatt
      121 gttgcccagt tcatgtccac ggtaatggag ctgccgatgt gcggccacgg cacgatctgc
      181 ctgttcactc acctgctcga atccggatta gtgtcgcttg acgaaaccga ttcctgcagc
      241 ttcgagttgc gcctgccaaa gtccagcgcc agggttgagc taagccgacg tggcgacaat
      301 cgaatccagg ttatgctcga tattcgagct ccgacattcg aaacagcgcc gccgcatacc
      361 gaagccctgc tgacgctgct aggtctcgac tctggcgcgc tcgacactac gctaccgctc
      421 gaaaccgcgc gcggggattt tattcacctg gtagtgccaa ttgcaggcct cgcagcaatg
      481 cgagcgatca agcccgattt cgacggcatg gtagaattct gcaacgctca cggcatcgag
      541 acgattgcgg cgttctgccg cgaagtcgaa aaccccgaaa acacgattca tgtgcgcgac
      601 ttttgcccgg cggtcggggt aagcgaatcg gccgcggccg ggaccaccaa cgcggcgctg
      661 accagttacc tgctgcggca cgccatgctg ccggccggga agagcgttgt cagcctgaac
      721 gcagaacagg gactcgaact cggtcggccc agttcgatac gatccattgt tacccctgga
      781 gatggcggaa tcgcgcgcct gcaggtcggc ggcgtagcga ccagggtttt cgacgggcag
      841 gtttatttat aggcggtatc ggatgacaac agacctcgaa caactttatc gggaagcgct
      901 ggtgtgggac gcgcatgcgg gcgtattccc gagtccccag gtcgatctga atctgctcga
      961 cgagtggcgt caactgggcg ccagttatgt cagcatcaac gtcggcttcg acgtcatgaa
     1021 ctggcaggaa accctggcca cgctcgcggc ctatcgccgc tgggtgctgc gaaacaccga
     1081 tcacttcgtg ctggccaacg acgcagtcga tatcgaccgc gcgcgggccg caggcaagct
     1141 cgcgctgagt ttcgatatcg agggcatgaa tgcgcttaac gaagatctca acatggtcgg
     1201 cctgtaccac gcccttgggg tgcggcaaat gctgttcgcc tacaacctga acaacgccgc
     1261 cgccggtggc tgtcacgatc gcgataccgg gctcactgcc ttcggccgcg aggtggtgca
     1321 ggaaatgaac cgcgtcggca tgatcgtcga ctgttcgcac gcgggttatc gcaccaccat
     1381 ggatatcatg caggtttcgc aaaagccggt tgtgttttca cactccaatc ccgcggcagt
     1441 ctggccgcat caacgcaaca tcgacgatga gcagatccgc gcctgcgccg cgaccggcgg
     1501 cgtcatcggg ctcaacggca tgggcatctt tctcggtgac aacgatatca gcgtcgacac
     1561 catggtgcgg cacgtatgct atgtctgcga tctcgtcggc agcgagcata tcggtatcgg
     1621 tttcgactac tcgcccggca tcgatgtcga tatcggggtt attctgcgca gccggcccga
     1681 ctactggcca gcgggtcaac agtacgacac cagggacatc aagcacggcg gcccggccca
     1741 gctgccggcg ctgatcgcca ggctagccga tgcgggattc gacgacgtgg acttgcgtgg
     1801 attcctcggg gaaaattttc gtcgagtagc gctcgaggcc tggagtggct gatcatcgag
     1861 gcccgctggg cggaatacat catcaccagg ctcagcagcg agtgccagag caccggttgc
     1921 gcgatcgtca cagaattatc ggttcggtga ttcctgcaac agctgcttga gctccgcgat
     1981 ttcggatcgc aacgacgcga cctcctggtg cagatcggcc tcgatatgat cccgggtctg
     2041 gtcgagcgcc tcttgccgtt cctgactctc ctgctcggta aaactgtgca tcgcgttgac
     2101 gataatcgcg ataaacaggt taagcatggt gaaggttgcg atcaagatga aaatgacgaa
     2161 gaagatcctc gcattcgggt gcacttccat taccgggcgt acgataccca tcgaccagct
     2221 ttccagcgtc atgatctgga atagcgagaa tagagattcg cctatgctgc cgaaccactg
     2281 tgggaaattg gcgccgaaca gcttggttgc tatgaccgcg aacacgtaat agagcaacat
     2341 cagcaccagc gcgatggaac ccaggcccgg gatagaatcc agcagcgctc cgacca
//
LOCUS       DATCZV010000363         4345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_82438, whole genome shotgun sequence.
ACCESSION   DATCZV010000363 DATCZV010000000
VERSION     DATCZV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82438"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /gene="clpA"
                     /locus_tag="VIW27_08150"
     CDS             <1..2206
                     /gene="clpA"
                     /locus_tag="VIW27_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY5739683.1"
                     /translation="ITVEHLLLMLTENDAATAVLEACGGDVQLLKAELEIFVDQNTPL
                     LMADEDRDTQPTLGFQRVLQRALFHVQSSGSGEVSGANVLVAIFSEQDSHAVYLLNKH
                     NIERLDVTNYLSHGITRVNEESEEETFTHEAETVPGDQENANPLDKFATNLNELAFSG
                     KIDPLIGRDQEIERIIQTLCRRRKNNPLLVGEAGVGKTAIAEGLARKIVEEEVPEVLL
                     ESTVYALDLGALVAGTKYRGDFEKRLKAVIGQLTREPGSILFIDEIHTIIGAGSASGG
                     VMDASNLIKPVLANGDIKCIGSTTYTEFRGIFEKDRALARRFQKIDVPEPNNEETYLI
                     LKGLKSRFEEHHNVKYTLQALRKATELASRYITDRHLPDKAIDVIDEAGAQRRLQAAK
                     QRKKTIGIHDIENIVAKIARIPANTVSTSDLDMLKNLGKNLSRVVFGQDKAIKALDSA
                     IKMSRSGLGRESKPVGSFLFSGPTGVGKTEVCRQIANIMGIELIRFDMSEYMERHTVS
                     RLIGAPPGYVGYDQGGLLTEELNKHPYSVVLLDEIEKAHPDVFNLLLQVMDNGTLTDS
                     NGRKADFRNCILVMTTNAGAEQMSRASMGFTLQDHTLDTGEAIKKTFTPEFRNRLDAI
                     IQFSALDINVILNVVDKFLIELESQLEAKKVSMTISDAARRWLAEKGYDPRMGARPMA
                     RVIQEEIKQVLADELLFGALTGGGRVKIDVKDGSLHCHVVKSRKRKESASVN"
     gene            complement(2220..2438)
                     /gene="infA"
                     /locus_tag="VIW27_08155"
     CDS             complement(2220..2438)
                     /gene="infA"
                     /locus_tag="VIW27_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY5739684.1"
                     /translation="MPKGDHIEMRGVVKDTLPNTMFRVELENGHVVTAHISGKMRKHY
                     IRILTGDTVTVELTPYDLTKGRITFRER"
     gene            2564..3505
                     /gene="fabD"
                     /locus_tag="VIW27_08160"
     CDS             2564..3505
                     /gene="fabD"
                     /locus_tag="VIW27_08160"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY5739685.1"
                     /translation="MTQYAYVFPGQGSQSVGMVSGWQDHQTVVEEVFRIAADNLGYDL
                     LTLVGEGPDSELNRTEITQPAMLCAGVACWRIASGQPEMPAPAVMAGHSLGEYTALVC
                     SGSIELGDAVRLVARRGQLMQAAVAPGEGAMAVIIGLGDDDVVAACARVDDGVVEAAN
                     FNAPGQVVIAGSAAAVERALQYAQEMGARRAKPIPVSVPSHCSLMANAAEELASELEQ
                     IEIRMPAVPVIHNQNAAPAASVAEIRQRLKLQLYQPVKWVGCVEAMHSRGVTTLIECG
                     PGKVLTGLTRRIDKSLQAYSVFDAASLASTQQSVGEN"
     gene            3507..4247
                     /gene="fabG"
                     /locus_tag="VIW27_08165"
     CDS             3507..4247
                     /gene="fabG"
                     /locus_tag="VIW27_08165"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY5739686.1"
                     /translation="MLTDQVALVTGASRGIGQAIALALGAQNAVVIGTATSQAGADGI
                     SAKLAENDIRGGGLTLDVADSESVAACIKQVTEEYGAPDILVNNAGITRDSLLMAMKD
                     EQWDEIVNTNLSSVFRMCKAVIRPMMKKRAGRIINISSVVGATGNPGQTNYAAAKAGM
                     VGFAKSLAREIGSRGVTVNTVAPGLIDTDMTRELPDEQRQALAAQIPLGRLGSGEEVA
                     AAVVFLASPAAAYVTGETIHVNGGMYMG"
BASE COUNT          947 a         1299 c         1332 g          767 t
ORIGIN      
        1 tattacggtc gagcatttgc tgttgatgct gaccgaaaac gatgccgcga ccgcggttct
       61 cgaggcctgt ggtggcgacg tgcagctgct caaggccgaa ctggagattt tcgtcgacca
      121 gaacacgccg ctgttgatgg ccgacgaaga ccgcgacacc cagccgacgc tcggtttcca
      181 gcgcgttctg cagcgggcgc tgttccacgt acagtcatcc ggcagcggcg aagtttccgg
      241 cgccaacgtg ctggtggcga ttttctcgga acaggattcg cacgccgtct acctgttgaa
      301 caagcacaat atcgagcgtc tcgacgtcac caattacctg tcgcacggaa ttacccgcgt
      361 caacgaggaa agcgaagagg aaaccttcac ccacgaagca gagaccgtgc ccggtgacca
      421 ggaaaacgcc aacccgctgg acaagttcgc gaccaacctg aacgaactcg ccttttcggg
      481 caagatcgat ccgctgatcg gccgcgacca agaaatcgag cgcatcattc agacgctgtg
      541 ccggcggcgc aagaacaacc cgctgctggt cggcgaagcc ggcgtcggca aaaccgcgat
      601 cgccgagggc ctggcgcgca agatcgtcga ggaagaagtc cccgaggtgc tgctggaaag
      661 tacggtttac gcgctcgatc ttggtgcact ggtagccggc accaagtatc gcggcgattt
      721 cgagaaacgc ctgaaggcgg ttatcggcca gttgacgcgc gagcccggat cgatcctgtt
      781 catcgatgaa atccatacca ttatcggtgc cggttcagcc tcgggcggcg tcatggatgc
      841 ctccaacctg atcaagccgg tgctcgccaa cggcgacatc aagtgtatcg gctcgaccac
      901 ctataccgag tttcgcggca ttttcgaaaa agaccgcgcg ctcgcgcgcc gctttcagaa
      961 gatcgacgtg cccgagccca acaacgagga gacttacctg atcctcaagg gcctcaaatc
     1021 gcgcttcgag gaacaccaca acgtcaagta cacgctgcag gcgttgcgca aggctaccga
     1081 gctggcatcg cgatacatca ccgaccgcca cctgccggac aaggccatcg acgtcatcga
     1141 cgaggccggc gcgcaacgtc gactgcaggc agccaaacag cgcaagaaga cgatcggtat
     1201 tcacgacatc gagaatatcg tcgccaagat cgcgcgcata ccggcgaaca ccgtcagcac
     1261 ctcggacctc gacatgctga agaacctcgg caagaacctg tcgcgcgtgg tgttcggtca
     1321 ggacaaagcc atcaaggcgc tggactcggc gatcaagatg tcgcgctccg gtctcgggcg
     1381 cgaatctaaa cccgtcggct cgttcctgtt ctccggtccg accggggtcg gcaagaccga
     1441 ggtctgccgg caaatcgcca atatcatggg tatcgagctg attcgcttcg atatgtcgga
     1501 gtacatggag cgtcataccg tgtcgcggtt gatcggcgcg ccgccgggat acgtcggtta
     1561 cgaccagggc ggcctgctga ccgaggaact caacaagcat ccctactcgg tggtgctgct
     1621 cgatgaaatc gagaaggcgc atcccgatgt cttcaacctg ctgctgcagg tgatggacaa
     1681 cggcacgctg accgacagta acgggcgcaa ggcggatttc cgcaactgca tcctggtgat
     1741 gaccaccaat gccggcgccg agcagatgag ccgtgcgtcg atgggcttca cgctgcagga
     1801 ccacacgctg gataccggcg aggcgatcaa gaaaaccttc accccggaat tccgcaaccg
     1861 gctcgacgcg atcatccagt tcagcgcgct cgacatcaac gtgattctca acgtcgtcga
     1921 caagttcctg atcgaactgg agtcgcagct cgaggccaag aaggtcagca tgacgatcag
     1981 cgacgcggcc cgccgctggc tggcggaaaa aggttacgac ccgagaatgg gcgcccgccc
     2041 gatggcgcgc gtgatccagg aagagatcaa gcaggtgctc gcggacgaat tgctgttcgg
     2101 cgcgctgacc ggcggcggca gggtcaagat cgatgtcaag gacggcagct tgcactgcca
     2161 cgtggtgaaa tcccgcaagc gcaaggaaag cgcctcggta aactgacgag aaaaggcgat
     2221 tatctttcgc ggaaagtgat acggcccttg gtcaggtcgt agggtgtcaa ctccacggta
     2281 accgtatcac cggtgagtat tcgaatgtaa tgcttgcgca tcttgccgga aatgtgcgcg
     2341 gtaaccacat gtccgttctc cagctcgacc cggaacatgg tgttgggcag cgtatccttg
     2401 actaccccgc gcatttcgat gtgatcgcct ttgggcataa tccctctctg tgtttataaa
     2461 gcgcgtcatt atccattatt taaccggtat atcaatagac ctggtaacgc ggccgaaaag
     2521 gctgtgataa catcggtgcc aagcaaataa atcaagctcg aatatgaccc agtacgccta
     2581 tgtattccct ggccagggat cgcagtccgt cggcatggtg agtggctggc aggatcacca
     2641 gactgtcgtc gaagaggttt ttcgaatcgc cgccgacaac ctcggttacg atttgctgac
     2701 cctggtcggc gaaggccccg acagcgagct gaaccgtacc gagattaccc agccggcgat
     2761 gctctgcgcg ggggtcgcct gctggcgcat cgccagcggc cagccggaga tgcccgcgcc
     2821 ggcggtgatg gccggtcaca gcctcggcga atacaccgcg ctggtctgca gcggcagcat
     2881 cgagctcggc gacgcggtca ggctggtcgc gcgccgcggc cagttgatgc aggcggcggt
     2941 cgcgccgggg gagggtgcaa tggcggtgat catcggactc ggcgacgatg acgtcgtcgc
     3001 tgcctgcgcg cgcgtcgacg acggcgtcgt cgaggcagcg aatttcaacg cccctggtca
     3061 ggtggtaatc gccggtagcg ccgccgcggt ggagcgcgcg ctgcagtatg cacaggagat
     3121 gggcgcgcgg cgcgcgaaac cgattccggt cagcgtaccc tcgcactgtt cgctgatggc
     3181 gaacgccgcc gaggaactgg cctcggaact cgaacaaatc gagatcagaa tgccggcggt
     3241 gccggtgatc cacaaccaga acgccgcgcc ggccgcgtcg gtggccgaaa ttcgccagcg
     3301 cctgaaactg caactgtacc agccggtcaa gtgggttggc tgtgtcgaag cgatgcactc
     3361 gcggggcgtc accacgctga tcgaatgcgg accgggcaag gtgctgacag gactgacgcg
     3421 gcgtatcgac aaatcgctgc aggcatacag cgtgttcgat gcggcttcac tcgcaagcac
     3481 gcaacaatcc gtaggggaga actgagatgc tgacagatca agtcgcgctg gtcaccggtg
     3541 ccagccgcgg aataggacaa gccatcgcgc tggcgctcgg tgcgcagaat gccgtggtca
     3601 tcggcaccgc gaccagccag gccggtgccg acggcatttc cgcaaagctc gccgagaacg
     3661 atatccgggg cggcgggttg acgctcgatg tcgccgactc cgagtcggtg gcggcctgca
     3721 tcaagcaggt caccgaggaa tacggcgcac cggatatcct ggtcaacaac gccggcatca
     3781 cccgcgattc gctgttgatg gcgatgaagg acgagcagtg ggatgagatc gtcaatacca
     3841 acctgagttc agtcttccgg atgtgcaagg cggtaatcag gccaatgatg aaaaaacgcg
     3901 cgggccgcat catcaacatt tcctcggtgg tcggcgccac cggtaatccg gggcagacca
     3961 actacgcggc cgccaaggca ggtatggtcg gcttcgccaa gtccctggcc cgtgaaatcg
     4021 gttcccgcgg cgtcaccgtg aacaccgttg cgccgggact catcgatacc gacatgaccc
     4081 gcgagctgcc cgacgagcag cgccaggcgc tcgccgcaca gattccgttg gggcgactcg
     4141 gtagcggtga agaagtcgcc gcggccgtgg tgttcctggc ctccccggcg gcggcctacg
     4201 tcaccggaga aaccattcac gtcaacggcg gaatgtatat gggatagtaa aagctttaag
     4261 ttaactgggt aactatctga tttttaaact atttcgaagg tcaaaaaaaa attcgacatt
     4321 gctggtaact aaaaccgctt ttcaa
//
LOCUS       DATCZV010000364         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_189291, whole genome shotgun sequence.
ACCESSION   DATCZV010000364 DATCZV010000000
VERSION     DATCZV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189291"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VIW27_08170"
     CDS             <1..861
                     /locus_tag="VIW27_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY5739687.1"
                     /translation="LFHAERGGIMCEFSITRLADDHYYLISAIGSENKDLDWMQKRAE
                     GYDVDIRNLTEDYAGMLVTGPKAREILQLMTEDDLSNEAFPWLAVREIKLDSAAVRAL
                     RVSYAGELGYELHMPSWQQLSIYDSLWRVGEPLGLRDFGGYAFNSMRMEKMYRAYGNE
                     FTEEISGLEAGMGRFIDLEHDFIGRDNLRQRQQDGYAIELAYLSFDDDVPCECFGNEA
                     IYHNGELIGLTTGGAYGHRVGRSLAFAYLKPALIRDGLEVTVDTSIGSRSAHIEMRAA
                     YDPDNQLLRS"
     gene            887..1456
                     /locus_tag="VIW27_08175"
     CDS             887..1456
                     /locus_tag="VIW27_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5739688.1"
                     /translation="MQTVNFIHMEDGTREDYALLERLEAEYNAELPARILDGLRRLEN
                     SFSGYQVDRLVHSLQGATRAHRAGESEEYVVAVLLHDIGDDLAPYSHSEMAAAILRPF
                     VSEKLHWIVKHHGLFQMYYYAHHCGGDRHARDVYKDHPWYQEAIDFCHQYDQNCFDPD
                     YDYEPLEFFEPILHRVLAKPRFEQSAAAG"
     gene            complement(1481..>2546)
                     /locus_tag="VIW27_08180"
     CDS             complement(1481..>2546)
                     /locus_tag="VIW27_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5739689.1"
                     /translation="LLQDKVRTRDTIARLGGDEFGLLLERCTLTQANRVAEAVRDAVG
                     NFRFNWAGRNFRLGASIGVIPINFSSGNITNVMRSADAACYAAKDEGRNRIHIYREDS
                     FEMAQRHQEMRRIVELNQAFDDDRFVLYQQPILALGTQQDAGGLFCEMLVRMVDEDGF
                     PVLPANFLPTAERYNLATQLDTRVVNGALDWLTVNPNVSCTINLSGISIADEDFLRFM
                     LEVIDEKAIEPQRLCLEITETAAIQNLSKANRFIEQLRERGCFFALDDFGSGLSSFAY
                     LKTLPVDYLKIDGFFVRDMINDRINFELVKSINDIGHVMGKQTIAEFVENQATLEALR
                     EIGVDYAQGYDIARPEPARG"
BASE COUNT          504 a          802 c          764 g          476 t
ORIGIN      
        1 ctgttccacg ccgagcgcgg cggcatcatg tgcgaattct cgattacccg cctggccgac
       61 gaccactatt acctgatctc ggcgatcggc tcggaaaaca aggacctgga ctggatgcaa
      121 aagcgcgccg agggttacga cgtcgacatt cgtaacctca ccgaggatta cgcgggcatg
      181 ctggtcaccg ggccgaaagc gcgtgaaatc ctgcagctaa tgaccgagga cgacctgtcc
      241 aacgaagcct ttccgtggct cgcggtgcgc gagatcaagc tcgacagcgc ggcggtgcgc
      301 gcgctccgcg tctcctacgc cggcgaactc ggctacgaat tgcacatgcc gtcgtggcag
      361 cagctgtcga tctacgattc cctgtggcgc gtcggcgagc cgctgggatt gcgtgatttc
      421 ggcggctacg cgttcaattc catgcgcatg gaaaagatgt accgcgccta cggcaacgag
      481 ttcaccgagg aaatcagcgg cctcgaagcc ggcatgggcc gtttcatcga cctggagcac
      541 gatttcatcg gtcgcgacaa cctcaggcag cgccagcagg acggctacgc gatcgagctc
      601 gcctacctgt cgttcgacga cgatgtgccc tgcgaatgct tcggtaacga ggccatctac
      661 cacaacggcg agctgatcgg cctgaccacc ggcggcgcct acggtcaccg cgtcggcagg
      721 agcctggcct tcgcgtacct gaagccggcg ctgattcgcg acggcctcga ggtcacggtc
      781 gacacctcga tcggcagccg cagcgcgcat atcgagatgc gagcggccta cgacccggac
      841 aaccagctgt tacgctcctg agcgccgcat tatcccggag attgccatgc aaaccgttaa
      901 cttcatccac atggaagacg gcacccgcga ggactacgcg ttgctcgagc gactcgaagc
      961 cgagtacaac gccgaactgc cggcacgcat tctcgacggc ctgcggcggc ttgaaaacag
     1021 tttttcgggc taccaggtcg atcgcctggt gcacagcctg cagggtgcga cgcgcgccca
     1081 ccgcgccggc gagtcggagg aatatgtcgt cgcggtgctg ctgcacgaca tcggcgacga
     1141 tctcgctccc tattctcaca gcgaaatggc ggccgcgatc ctgcgtccgt tcgtctctga
     1201 aaagctacac tggatcgtca agcatcacgg tctgttccag atgtattact acgcccatca
     1261 ctgcggtggc gaccgccacg cgcgggatgt ctacaaggat catccgtggt accaggaggc
     1321 gatcgatttt tgccatcaat acgaccagaa ctgcttcgac cccgactacg attacgaacc
     1381 gctggagttt ttcgagccga tcctgcaccg cgtgctcgcc aaaccgcgat tcgagcagtc
     1441 cgccgcggcc ggttaagcgg gccgcctggg gcgccgcggg ctaacccctt gcgggctcgg
     1501 ggcgcgcgat atcgtaaccc tgggcatagt cgacaccaat ctcgcgcagc gcttccagcg
     1561 tcgcctggtt ttcgacgaac tcggcgatgg tttgcttgcc catcacatgg ccgatgtcat
     1621 tgatcgattt caccagctcg aaattgatac ggtcgttgat catgtcacgc acgaagaaac
     1681 cgtcgatctt cagataatcg accggcaggg ttttcaggta ggcgaaagac gacaggccgc
     1741 tgccgaaatc gtcgagcgcg aaaaaacagc cgcgttcgcg caattgctcg atgaatcggt
     1801 tggccttgct gagattctgg atcgccgcgg tctcggtgat ctccaggcac aggcgctgcg
     1861 gctcgatcgc cttctcgtcg atgacctcga gcatgaagcg caggaagtcc tcgtcggcga
     1921 tcgatatccc tgacaggttg atcgtacagc tgacattcgg attcacggtc agccagtcga
     1981 gggcgccgtt caccacccgc gtatccagct gcgtcgccag gttatagcgc tccgcagtcg
     2041 gcagaaaatt ggcgggcaat accggaaagc cgtcctcgtc gaccatacgc accaacatct
     2101 cgcaaaacaa gccacctgcg tcctgctggg taccaagcgc gaggatcggc tgctggtaga
     2161 gcacgaaacg gtcgtcgtcg aaggcctggt tgagttcgac gatgcggcgc atttcctggt
     2221 ggcgctgcgc catctcgaag ctgtcctcgc gatagatatg gatgcggttg cgaccctcgt
     2281 ccttggccgc gtaacaggcg gcgtcggcag agcgcatgac attggtgatg ttgccgctgg
     2341 aaaaattgat cgggatcacg ccgatgctcg cgccgagccg aaaattgcgg ccggcccagt
     2401 tgaaacggaa attgccgacc gcatcgcgaa ccgcctcggc gacgcgattt gcctgggtca
     2461 gcgtacatcg ctcgagcaac agcccgaact cgtcgccgcc gaggcgcgcg atggtgtcgc
     2521 gggtacgcac cttgtcctgc aacagc
//
LOCUS       DATCZV010000365         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_294237, whole genome shotgun sequence.
ACCESSION   DATCZV010000365 DATCZV010000000
VERSION     DATCZV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294237"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2214)
                     /locus_tag="VIW27_08185"
     CDS             complement(<1..>2214)
                     /locus_tag="VIW27_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5739690.1"
                     /translation="DRIVLDSRNARLGIVTTGKSYLDTLQALADLGIDERLAKKIGIR
                     LYKVGMSWPLDPVMAHKFAEDLEEILVIEEKRSVIEDQLMGQLYNWPVHRRPRVVGEY
                     DENRNLLVTNLGELTPGMIARVIAARVSRFFDSESIHQRLAFLEAKEARLAQPRARAE
                     RKPWYCSGCPHNTSTVVPEGSLAYGGIGCHYMATWMDRGTVTFTQMGGEGATWLGQAP
                     FTERKHVFQNLGDGTYFHSGLLAIRAAIAAGVNITYKILYNDAVAMTGGQPFDGSLTV
                     EQIIEQLRAEGVKRIALVSDQPEIYRGSRAAGLDGVSLTHRDRLDEIQRELRDTAGCT
                     ALIYAQTCAAEKRRRRKKGILVDPPKRALINHAVCEGCGDCGLQSNCLSIIPRQTWLG
                     RKRAIDQSACNKDFSCLKGFCPSFVTVHGGRLRKQRGTPSDDSWSLPPEPAKLPVEDD
                     YSILITGIGGTGILTVGSVLGMAAHIEERQVSVLNQTGLAQKFGAVTGHVRIAGSRKP
                     IYSVRIPSGEANLLLGADLVVSAGNDALAKLNRETSHAVVNNHLSPTAEFTRNADAVF
                     PLHDMQQSILDELEPERGHFVDATKLATALLGDAIFTNFFLLGVAYQQGLVPLGAAAI
                     EQAIELNGVAVEQNRQALLWGRRYAFDPAAVRRAAGLDRERQAVDGETGLDDLLARFA
                     ESLTAYQDQALAARFLAAVERVRAAEETWRQQSTDGELPLTEAVARSYHKLLAYKD"
BASE COUNT          364 a          700 c          732 g          418 t
ORIGIN      
        1 gtccttgtag gcgagcagct tgtggtagct gcgtgccacc gcctcggtca gcggcagttc
       61 gccgtcagtc gattgctgcc gccaggtttc ctcggccgcc cgcacgcgtt cgacggccgc
      121 cagaaaacgc gccgccaatg cctggtcctg gtaggcggtc agcgactcgg cgaaccgcgc
      181 cagcaaatcg tcgagacccg tctcgccatc gactgcctgg cgctcgcggt ctagcccggc
      241 cgcgcgccgt accgccgcgg gatcgaacgc ataacggcgg ccccacagca gtgcctgccg
      301 gttttgctcg accgccacgc cgttgagttc gatggcctgt tcgatggctg ccgcgccgag
      361 cggtaccagc ccctgctggt aagccacgcc gagcaggaaa aaattggtga agatcgcatc
      421 gccgagcagc gcggtcgcga gtttggtggc atcgacgaaa tgaccgcgtt cgggctccag
      481 ttcgtcgagt atcgactgct gcatgtcgtg caacgggaag accgcgtcgg cattgcgggt
      541 aaattccgcg gtcggcgaca ggtggttgtt gaccacggcg tgcgaggtct cgcggttgag
      601 cttggcgagc gcatcgttgc ctgccgagac caccaggtcg gcgcccaaca gcaggttggc
      661 ctcgcccgag ggtatgcgta ccgagtagat cggctttcgc gagccggcga tgcgcacgtg
      721 gccggtcacg gcgccgaatt tttgcgccag gccggtctgg ttgagcaccg agacctggcg
      781 ctcctcgatg tgtgccgcca tgccgagtac cgagccgacg gtgaggatgc cggtgccgcc
      841 gatgccggtg atcaaaatgc tgtaatcgtc ttcgaccggc agcttggcgg gctcgggagg
      901 aaggctccag ctgtcatcgc tcggcgtacc acgctgcttg cgcaagcggc cgccgtggac
      961 ggtgacgaag ctagggcaaa accccttcag gcatgaaaag tccttgttgc aggcgctctg
     1021 gtcgatcgcc cgtttgcgac ccagccaggt ttgccgcgga atgatcgaca ggcaattcga
     1081 ttgcagaccg caatcgccac aaccctcgca gaccgcgtga ttgattaaag cccgtttcgg
     1141 cggatcgacg aggatgccct ttttacgccg gcggcgtttc tcggcggcac aggtctgcgc
     1201 gtagatcagc gccgtgcagc ccgctgtatc gcgcagttcg cgctgaatct cgtcgagccg
     1261 gtcgcgatgc gtcagcgaaa cgccgtcgag tccggcggcg cggctgccgc ggtagatttc
     1321 cggctggtct gaaaccagcg cgatgcgctt gacgccctcg gcgcgcagtt gctcgatgat
     1381 ttgctccacc gtcaggctgc cgtcgaacgg ctggccgccg gtcatcgcca ccgcgtcatt
     1441 gtaaagaatc ttgtaggtga tattgacgcc ggcggcgatc gcggcgcgga tcgccagcag
     1501 cccggaatgg aaataggtgc cgtcgccgag attctggaat acgtgttttc gttccgtgaa
     1561 cggcgcctga ccgagccagg tggcgccctc gccgcccatc tgcgtaaagg tcacggtgcc
     1621 gcggtccatc caggtcgcca tgtagtggca gccgataccg ccataggcaa ggctgccctc
     1681 ggggaccacg gtggaagtgt tgtgcggaca gccggagcag taccagggct tgcgctcggc
     1741 gcgcgcgcgc ggctgcgcga gcctggcttc cttggcttcg aggaaagcga gccgctggtg
     1801 gatcgattca ctgtcgaaaa aacgcgacac gcgcgccgcg ataacgcgcg cgatcatgcc
     1861 cggcgtcagt tcaccgagat tggttaccag caggttgcgg ttttcgtcgt actcgccaac
     1921 cacccgcggc cggcggtgca ccggccagtt gtagagttgc cccatcaact ggtcctcgat
     1981 cacgctgcgt ttctcctcga tgacgaggat ttcctcgagg tcctcggcga acttgtgcgc
     2041 cattaccggg tccagcggcc agctcatgcc gaccttgtag aggcgaatgc cgatcttctt
     2101 tgccagccgc tcgtcgatac ccaggtcggc gagcgcctgc agcgtgtcga ggtaggattt
     2161 gccggtggtg acgataccca gacgtgcatt gcggctgtcg agcacgatgc ggtc
//
LOCUS       DATCZV010000366         4471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_242052, whole genome shotgun sequence.
ACCESSION   DATCZV010000366 DATCZV010000000
VERSION     DATCZV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242052"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VIW27_08190"
     CDS             complement(<1..461)
                     /locus_tag="VIW27_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5739691.1"
                     /translation="MSNEQENSVDRRQLVSALIDGLPQQALVISGLGSPSYDLFAAGD
                     RELNFYLWGAMGGASSLALGLALAQPARPVLAITGDGEQLMGIGSLATIGAQQPDNLT
                     LVVLDNGHYGETGMQPSHTSLGTDLVAVARGFGIDDAFGVDSLEQVGEILRR"
     gene            complement(454..993)
                     /locus_tag="VIW27_08195"
     CDS             complement(454..993)
                     /locus_tag="VIW27_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEY5739692.1"
                     /translation="MSAPQPDKHWQQAVFDTLKAGGIRQLAYVPDAGHATAIRAAQAD
                     PDIRDVLLTTEEEGVALACGAWLGGQRAALLMQSSGVGNCVNMFSLLRNCRFPFLTIV
                     TMRGEYAEFNPWQLPMARSTPAVLESMGITVLRVSEPAAVAETVGAAVDAVFEAGEQV
                     AVLLSQSLIGRKQWINADE"
     gene            complement(1143..2039)
                     /locus_tag="VIW27_08200"
     CDS             complement(1143..2039)
                     /locus_tag="VIW27_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739693.1"
                     /translation="MPDNLKGIAYLVMASVGFSTMALLIKLLGQHLQVTQILLLRQLG
                     MLVLVSPAILRNFPGSLRTSRPGLHITRIACALVAMLCGFTAVIHLPLADATALFFAK
                     SFFLTIFAMLILGEQVGRYRWGAVVVGFGGVLVMLQPGTENFSIYSLASLAGAAGAAG
                     VMINLRILSQSDSANTILTWSALGVGIAMTVPGIYYWQAPTPFEWGLIAALALVSLLS
                     QKGNIFAYKYGEASLLASLDYVRLLWATLLGWMIFDQLPGAATWIGAVIVVAAAVFTI
                     YRETRRKQNPDAGPAARSTGET"
     gene            complement(2218..3147)
                     /locus_tag="VIW27_08205"
     CDS             complement(2218..3147)
                     /locus_tag="VIW27_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5739694.1"
                     /translation="MANLVIVEHDNNEVAAATLHAVTAAAQIGGDIDLLVAGENCGAA
                     AAAAAKIVGVRAVKVADDAKFAHGLAENLATLIVDIAGDYSHLLASATTFGKNVMPRA
                     AALLDVAQISDIVEVKSDDTFVRPIYAGNALATVQSSDRIKAITVRTIKFEPAAAEGG
                     SASVESVSGGSDTGLSSWVSQALSDNDQVDLTSAKIVISGGRGMQSGDNFEMLHKIAA
                     KLGAAVGASRAAVDSGFVPNELQVGQTGKVIAPDLYIAVGISGAIQHLAGMKESKVIV
                     AINNDSEAPIFQVADYGLVADLFEAVPELDAAL"
     gene            complement(3150..3899)
                     /locus_tag="VIW27_08210"
     CDS             complement(3150..3899)
                     /locus_tag="VIW27_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY5739695.1"
                     /translation="MKVLVCVKRVLDPQVKARVKADGSGVDLANVKMTMNPFCENALE
                     EALRMREAGVVEEIIVAAVGGGKTEETLRHGLAMGADRAIHVVTEDSLEPLAIAKVLK
                     RVAEQQDVGLVLTGKQAIDGDSNQTGQMLSALLGWSQATYCSNIKIDGEQATIVREVD
                     AGLETIQVKMPAVMSADLRLNEPRYVKLPDVMKAKRKPMEKIELDSLGIDTANRLTTL
                     QVDEPEGRAAGIIVNSVAELVEKLRNEAKVI"
BASE COUNT          855 a         1462 c         1358 g          796 t
ORIGIN      
        1 cgccgcagga tttcgccgac ctgctcaagg ctgtcgaccc cgaaggcatc gtcgatgccg
       61 aaaccgcgcg ccaccgccac caggtcggtg ccgaggctgg tatgactggg ctgcatgccg
      121 gtttccccgt agtggccgtt atcgagcacc accagggtca ggttatcggg ttgctgggca
      181 ccgatggtgg ccaggctgcc gatgcccatc aactgctcgc cgtcgccggt aatcgccagt
      241 accgggcgcg cgggctgcgc cagcgcgagg ccgagcgcaa ggctcgatgc accacccatc
      301 gcgccccaca ggtaaaaatt cagctcgcgg tcgccggctg cgaacaggtc gtaactcggc
      361 gatcccagac cgctgatcac cagcgcttgt tgcggcagcc cgtcgattag cgcgctgacc
      421 agttggcggc gatcgacgga attttcctgc tcgttactca tcggcgttaa tccattgctt
      481 gcggccgatc agcgactgcg acagcaatac tgcgacctgt tcgccggcct cgaacaccgc
      541 gtcgacggcg gcgccgacgg tctccgcgac cgccgcgggc tcactcacac gcagcacggt
      601 gatgcccatc gactcgagca cggccggggt actcctggcc atcggcaact gccacggatt
      661 gaactcggcg tactcgccgc gcatggtgac gatagtcaaa aacggaaacc ggcaattgcg
      721 cagcagcgaa aacatgttga cgcagttgcc gaccccgctg ctctgcatca gcaaggccgc
      781 gcgctggccg ccgagccacg cgccgcaggc cagcgcgacg ccctcttcct cggtggtcag
      841 cagcacgtcg cgaatatctg ggtcggcctg cgccgcgcga atcgcggtcg cgtggccggc
      901 atcgggcacg taggcgagct gtcgaatgcc acccgctttc aacgtatcga agacagcttg
      961 ctgccagtgc ttgtcaggct gcggcgcgga catgacgctc ccgatgacgc ggctcaacaa
     1021 ctgggcaggc aaacggcagt actgggaaat tcatctgggc agcgatcccg gttcgacgaa
     1081 caccggtgca gattgccacg gcgcggcggt ccccgcaata agcggttttc gatccgacgg
     1141 cttcaggttt cgccggtgct gcgcgccgcc ggccccgcgt ccgggttctg cttgcgccgc
     1201 gtttcgcggt agatcgtgaa caccgccgcg gcaaccacga taacggcacc gatccaggtc
     1261 gcggcacccg gtaactgatc gaaaatcatc caaccgagca gcgtcgccca cagcaggcgc
     1321 acatagtcga gcgacgcgag cagcgatgcc tcaccatact tgtaggcaaa gatgttgcct
     1381 ttctgcgaaa gcaaagagac taacgccaac gccgcaataa ggccccattc gaaaggcgtt
     1441 ggtgcctgcc agtaataaat cccgggcacc gtcatcgcga tgccgacacc gagcgccgac
     1501 caggtgagga tcgtgttggc gctgtcgctt tgcgacagga tgcgcaggtt gatcatcacc
     1561 ccggccgcac cggcggcacc ggccagcgag gcgaggctgt agatcgagaa attctcggtg
     1621 ccgggttgca gcatgacgag cacgccgccg aagccgacca ccaccgctcc ccagcggtag
     1681 cggccgacct gctcgccgag aatcagcatc gcgaaaatcg tcaggaagaa gctcttggcg
     1741 aagaacagcg ccgtggcatc ggccagcggc agatggatca ccgcggtgaa accgcacaac
     1801 atcgcgacca gcgcgcaggc gatgcgggtg atatgcagcc ccggccgcga ggttctgagc
     1861 gagcccggaa aattgcgcag tatcgccggc gacaccagca ccagcatgcc gagctggcgt
     1921 aacagcagga tctgcgtgac ctgcaaatgc tgcccgagta acttgatcag caatgccatg
     1981 gtcgaaaagc cgaccgacgc catcaccagg taggcgatgc ccttgaggtt atcgggcaaa
     2041 cggtgaaaac gattcaatat ccaggccgta gcagcggttg gcgacgaccg gcggcgactc
     2101 gagacagcgc cgtcggctcg aatgagcggt gattgtcgcc aatccagacc cgcggcgcaa
     2161 aaaaaaccgc cgctcgacag agcggcggcg aacgatcgct acagaaccgg ttccgcgcta
     2221 aagcgcggcg tcgagttccg gtaccgcttc gaacagatcg gcgaccagcc cgtagtcggc
     2281 gacctggaag atcggcgcct cggaatcgtt gttgatggcg acgatgacct tgctttcctt
     2341 catgccggcg agatgctgga tcgcgcccga gatgcccacc gcaatgtaca ggtccggagc
     2401 gatgaccttg ccggtctggc cgacctgcag ctcgttgggc acgaagcccg aatccaccgc
     2461 cgcacgcgac gcgccgaccg cggcgccgag cttggcggcg atcttgtgca gcatttcgaa
     2521 gttgtcgccc gactgcatgc cgcggccgcc ggagatgacg atcttggccg aggtcaggtc
     2581 gacctgatcg ttgtccgaca gcgcctgcga aacccagctc gacagcccgg tgtcgctacc
     2641 gccggaaacc gattcgacgc tggcggaacc gccctcggcg gcggccggct cgaacttgat
     2701 ggtgcgcacg gtaatcgcct tgatcctgtc gctggactgc accgtggcaa gcgcattgcc
     2761 cgcgtaaatc gggcgcacga aggtgtcgtc cgatttgacc tcgacaatat cggaaatttg
     2821 cgcgacgtcg agcagcgcgg ccgcgcgcgg catcacattc ttaccgaagg tcgttgctga
     2881 cgccagcagg tggctgtaat cgccggcgat atcgacgatc agggtggcga ggttctcggc
     2941 cagtccgtga gcgaatttgg cgtcgtcggc gaccttgacc gcacgcaccc cgacgatttt
     3001 cgccgcggcc gcggcggcgg cgccacagtt ttcgccggca accagcaggt cgatatcgcc
     3061 gccgatctgt gccgccgcgg tgaccgcgtg cagcgtcgcg gccgcgactt cgttgttgtc
     3121 gtgttcgacg atgacgagat ttgccatgtt taaatcacct tcgcttcgtt gcgcagcttt
     3181 tcgaccagct cggccacgct gttgacgata atgccggcgg cgcggccttc cggctcatcg
     3241 acctgcaggg tcgtcaaccg gttcgcggta tcgatgccga gactgtcgag ctcgatcttt
     3301 tccatcggct tgcgcttggc tttcatgacg tcgggcagct tgacgtaacg cggctcgttg
     3361 agacgcaggt cggcgctcat caccgccggc atcttcacct ggatggtttc caggccggca
     3421 tcgacctcgc gcacgatggt cgcctgctcg ccgtcgatct tgatattcga gcaataggtg
     3481 gcctgcgacc agccgagcag cgcggacagc atctgcccgg tctggttgga atcgccgtcg
     3541 atagcctgct tgccggtcag caccaggcca acgtcttgct gctcggcaac ccgcttcagc
     3601 accttggcga tcgccagcgg ctcgaggctg tcctcggtga ccacgtggat cgcgcggtcg
     3661 gcacccatcg cgaggccatg gcgcagcgtt tcctcggtct tgccgccgcc caccgcggcg
     3721 acgatgattt cttcgaccac accggcctcg cgcatgcgca gcgcctcttc cagcgcgttt
     3781 tcgcaaaacg ggttcatggt catcttgacg ttcgccaggt cgacgcccga tccgtccgcc
     3841 ttgacgcgcg ccttgacctg cgggtcgagc acgcgcttca cacataccag tactttcatt
     3901 gctgccctca cgttcaagct tcggctcagg ggtcgcgtat tctatcgaca atcggcggcg
     3961 aatcaagcga tcacaggcga gatggcgcgt cgccctgatg acgcgattat tgaccggcac
     4021 cgaataaccc tgacgaggcg acggatatta tcataccccc cacccgcttg ggatttacgc
     4081 gcgactgccg attgtctgta tgcgccatgc gggccgcatt ccaggccgat gttgcgacgc
     4141 cgtcgagcgc aagcggtgca cttcgcgcct ggacgcgcgt gccctgcaca gcgttttcgc
     4201 ccgccaaaag actcgaaggc catcgcagca gcgcgcaggg cgccgaatat aatctaaact
     4261 ccctgctggc ctgcccggtc ctcgaggttg atgttcaacc gccgtcaaac cgaaaaaaac
     4321 catgaaaaat agttgacaaa tctttttttg ggattatatt cccaaattaa gcagcaacgc
     4381 tgccctattt taatctgaca cgacccatgg aggtccggaa atgaaactac ctgaaaacat
     4441 gacactaggc gtcaaaggcc tggtcacaac c
//
LOCUS       DATCZV010000367         2380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_612434, whole genome shotgun sequence.
ACCESSION   DATCZV010000367 DATCZV010000000
VERSION     DATCZV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612434"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VIW27_08215"
     CDS             complement(<1..426)
                     /locus_tag="VIW27_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY5739696.1"
                     /translation="MHALLFGFSLGFSLILAIGAQNAFVLRQGLRDEHLLLVCSICAF
                     SDALLILAGVSGFHLLVATFPSVTEVARYGGALFLGVYGLLSFFRAARVDEGLQPSEL
                     RSSSAWQAALTCLAFTWLNPHVYLDTVVLLGSVSTQFGDR"
     gene            462..1634
                     /locus_tag="VIW27_08220"
     CDS             462..1634
                     /locus_tag="VIW27_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017028649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739697.1"
                     /translation="MLRQQLQKPEIFLYLFAAAVPVSFAAWQALINNFSIEQAGFTGI
                     EIGILQSLREVPGFLAFAVVFLLLLMREQTIAFVSLLALGIGTAITGMLPTVIGLYFT
                     TVLMSVGFHYAETIMQSLSLQLIPVERLPLVLGRIIAVGSFVGLAVFGLLYIAVEWLA
                     LDYLWIYLLFGAGTLVIATLMYFGCPHFAGEAEQHKTMVLRRRYWLYYLLTFLSGARR
                     QIFVVFAGFLMVEKFGLTAGQMTLLFLVNGVLTIYLAPRIGRLVAWWGEKRTLTLEYA
                     GLVVIFTCYAFADSVWLAAVLYILDHVFFAMAIALKSYLKKIADPADMAATAGVSFSI
                     NHIAAVVLPFVLGMVWIASPALVFIIGAIFAAASLLLSQLVPGTPDKGMETVFSHP"
     gene            1658..>2380
                     /locus_tag="VIW27_08225"
     CDS             1658..>2380
                     /locus_tag="VIW27_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5739698.1"
                     /translation="MVTGAASGIGRAIALRFAAEGARVVMLDPRSDPIEGGESTLELI
                     RDAGGEAQALPVDVSDWAQLDAAVGAVVAEHGALHVMVNNAAIYTGTDLLATTAEDWD
                     RVMAVNLRGVFFGCKRAVQQMLTQQPLGEARGRIVNISSQHGMIACPGDIAYGVSKAG
                     CVYITRQVAADYAARGIVCNAVAPGKILTGRTDAIDEASMRYSRSRTPMAGPPLGRLG
                     KPADVASAALFLASDDCTYVNGA"
BASE COUNT          394 a          751 c          764 g          471 t
ORIGIN      
        1 ccggtcgccg aactgggtcg acaccgagcc cagcagtact accgtgtcga ggtagacatg
       61 ggggttcagc caggtgaagg cgaggcaggt cagcgccgcc tgccaggcgc tgctgctgcg
      121 cagttcgctg ggctgcaatc cctcatcgac gcgtgccgcg cggaagaaac tgagcaaccc
      181 gtaaacgccg aggaacagcg ctccgccgta gcgcgcgacc tcggtcaccg acggaaacgt
      241 cgccaccagc aggtgaaagc cgctgacgcc ggcgagaatc agcagcgcgt cggagaatgc
      301 acagatcgag cacaccagca gcaggtgttc gtcgcgcagt ccctgcctga gcacgaaggc
      361 gttctgcgcg ccgatagcga ggatcagcga aaagccgagc gagaagccga acagcagggc
      421 atgcaattga ttattccgcc tcgaggcatc aaaatccgtg catgttaagg cagcagctgc
      481 aaaaacccga aatctttctc tacctgttcg ctgccgcggt gccggtcagt ttcgcggcct
      541 ggcaggcgct gatcaataat ttttcgatcg aacaggccgg cttcaccggc atcgaaatcg
      601 gtatcctgca gagcttacgc gaggtgccgg gttttctcgc cttcgcggtg gtcttcctgc
      661 tgctgctgat gcgcgagcaa accatcgcct tcgtgtcgct gctggcgctc ggtatcggta
      721 ccgcgatcac cggcatgctg ccgaccgtga tcggcctgta cttcaccacc gtgctgatgt
      781 cggttggctt tcactatgcc gagaccatca tgcaatcgct gtcgctgcag ttgatacccg
      841 tcgaacgatt gccgctggtg ctcgggcgca tcatcgcggt cggctcgttc gttgggctcg
      901 cggttttcgg gctgctgtac atcgcggtcg aatggttggc tctggactat ttgtggatct
      961 acctgctgtt cggcgccggc acattggtca tcgccacgct gatgtatttc ggttgcccgc
     1021 atttcgcggg cgaggccgag cagcacaaga ccatggtgct gcgccgccgc tactggctgt
     1081 attacctgct gaccttcctg tcgggtgctc gccggcagat cttcgtcgtc ttcgcgggct
     1141 ttttgatggt cgaaaagttc ggcctcacgg ccggccagat gacgctgttg tttctggtca
     1201 acggtgtgtt gacgatttat ctcgcgccgc gcatcggccg gctggtcgcc tggtggggcg
     1261 agaaacgcac actgacgctc gagtatgccg ggctggtggt gattttcacc tgctacgctt
     1321 ttgccgacag cgtctggctg gcggccgtcc tgtatatcct cgatcacgtg tttttcgcga
     1381 tggcgattgc gctcaagagc tacctgaaga aaatcgccga tcccgcggat atggcggcca
     1441 ccgccggggt gtcgttctcg atcaatcaca tcgccgcggt ggtattgccg ttcgtgctcg
     1501 gcatggtgtg gatcgcctcg ccggcgctgg tgtttatcat cggcgcgatt tttgccgccg
     1561 cgtcgctgct gttgtcacaa ttggtgccgg gcacgcccga caagggcatg gaaacggtgt
     1621 tttcgcaccc gtgaaacggc tcgccgacag aacctgcatc gtgactggcg cggcctcggg
     1681 tatcggccgc gcgatcgcgc tgcgcttcgc ggccgagggc gcgcgggtgg tgatgctcga
     1741 tccgcgcagc gatccgatcg aaggcggcga atccactctc gaactcattc gcgatgccgg
     1801 cggcgaggcg caggcgctgc cggtcgacgt gtccgactgg gcgcaactcg acgccgcggt
     1861 cggcgcggtg gtcgccgaac acggcgcgct gcacgtgatg gtcaataacg cggccatcta
     1921 taccggcacc gatttgctgg cgaccacggc agaggactgg gaccgggtga tggcggtcaa
     1981 cctgcgcggt gtattcttcg gctgcaagcg cgcggtacaa caaatgctca cccagcagcc
     2041 gctcggcgag gcgcgcggcc gcatcgtcaa catatcctcg cagcacggca tgatcgcctg
     2101 cccgggcgac atcgcctacg gcgtcagcaa ggccggctgc gtctacatca cgcggcaggt
     2161 cgcggccgat tacgcggccc gcggcatcgt ttgcaatgcg gtcgcaccgg gcaagatcct
     2221 gaccggcagg accgatgcca tcgacgaagc ctcgatgcgt tattcgcgct cgcgcacgcc
     2281 gatggcgggc ccgccgctcg gccgcctcgg caagccggcc gacgtcgcca gcgccgcgct
     2341 gttcctcgcc agcgacgact gcacttacgt caatggcgcc
//
LOCUS       DATCZV010000368         4698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_772012, whole genome shotgun sequence.
ACCESSION   DATCZV010000368 DATCZV010000000
VERSION     DATCZV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772012"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VIW27_08230"
     CDS             complement(<1..99)
                     /locus_tag="VIW27_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014949932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY5739699.1"
                     /translation="MFEWISSPEAWIALATLVALEIVLGIDNIIFIS"
     gene            complement(220..507)
                     /locus_tag="VIW27_08235"
     CDS             complement(220..507)
                     /locus_tag="VIW27_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HEY5739700.1"
                     /translation="MANEGYHEPIEELSDETRDMHRAITSLMEELEAVDWYNQRVDAC
                     KDKELAAILAHNRDEEKEHAAMVLEWIRRRDPALDKELRDYLFTENPIAHD"
     gene            695..2032
                     /locus_tag="VIW27_08240"
     CDS             695..2032
                     /locus_tag="VIW27_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5739701.1"
                     /translation="MEFDELEPAADQALQTISNALTHPGTYTQLGIILGIYAVAFIVA
                     NRLRRVAGHFNISPQQAEGQPLKLFVVRLMNLIFPLLAILLLRVFVEISETLLAQGWL
                     MEAALTIAIVLLFNSIIDDFVRHPLVRGVLRYLGNPLLILHLLGVLDGLIEILESISI
                     SIGNIEISAYGIARVAIFGTLLFWIGRFSNRTGRELINRQSNLDSRTREVAGKLLEIS
                     VFFVIFLLLLQVMGINLTALAVFGGAIGVGLGFGLQAIASNFISGIIILLDRSLSVGD
                     YIEFDEQHRGVVRELNMRSTTLETYDGCDIMVPNEKFIVETFINWTHKDKRQRYRVDF
                     SVAYDSDIHKLVDIVRKVVASHPQVISGAEVAAEEQPDCEIDSFGDSGINMFVEFWMI
                     GIDDGKNRVGGDLLLMILDALREHEFEIPFPQREVRVLNPAFSFNAPKRVDPK"
     gene            complement(2046..2321)
                     /locus_tag="VIW27_08245"
     CDS             complement(2046..2321)
                     /locus_tag="VIW27_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739702.1"
                     /translation="MKTILEKYLNCEVGINIENAFRFEPAIITAVEDDHFSVVDEKQG
                     YTHHFSYGCIVQVIESDGGVDVGGFMMHKHFSVAIKVGHLMPLGPGY"
     gene            2474..2776
                     /locus_tag="VIW27_08250"
     CDS             2474..2776
                     /locus_tag="VIW27_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014110597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY5739703.1"
                     /translation="MNQISRRKMLGLSAAALIVGPALAMNSRSSLAAELPKLDPNDPQ
                     AKGLSYVHKSAKADNYCGNCQLYTGDPAASWGPCQIFPGKQVASAGWCSAWVKKAG"
     gene            complement(2930..4201)
                     /locus_tag="VIW27_08255"
     CDS             complement(2930..4201)
                     /locus_tag="VIW27_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5739704.1"
                     /translation="MNDSSPKLPTAAEVIVVGGGIAGIAAAWFLQRAGIEVAVCEKGV
                     VAGEQSSRNWGWCRQMGRDQAELPIVIESLRLWQEIADDVDTDIGFRRSGSLYLCESD
                     RDLANYDHFMNFAPDYGLDTRRLHRKQLEAVFPHCPPRWRSALHTPDDARAEPSLAVP
                     AMARTLRARGVPIIENCAVNGIDTQNGRVSSVATEHGDIETSTVLIAAGAWSTFLLKS
                     CGIRLPQLTVKASVARTAAMPSIYDGNASGSEVSFRRRLDGGYTIAIKGHLEILPSPA
                     HLAFARDFLPLLRTSLGTLSLRPPGLVVDGNFSRHRSLDPQPRDKVVARIKALLADRV
                     PAFAGVELAESWAGMIDALPDVVPVLDRAANLDGLWISTGFSGHGFGIGPAAGRVMAD
                     LIQGNSAGHDLQRFRLSRFSDGSKLELGPAI"
     gene            4297..>4698
                     /locus_tag="VIW27_08260"
     CDS             4297..>4698
                     /locus_tag="VIW27_08260"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5739705.1"
                     /translation="MEAIPQTSSFGAEVTGLDLSQPLDARRFERLKRMLLDYQVLLFR
                     DQQISPRHYQDFARRFGRLQEYLFAEGLDGFPYITEIVKTENETGGFGDFWHSDSAYL
                     ETPPSITMLYARQVPPHGGDTLFKDMYAVYDE"
BASE COUNT          876 a         1434 c         1440 g          948 t
ORIGIN      
        1 cgagatgaag atgatattgt cgatgccgag cacgatttcg agcgcgacca gtgtcgccag
       61 tgcgatccag gcctcggggc tgcttatcca ttcgaacatg gggggttccg tgggtcgatt
      121 cgggaaccct gattatatgc tgccgggcgc gcctatacag gtcggcggcg atgcctggcg
      181 ctcgagatcg cggccgcggc gaccgcgaat gttcgatatt tagtcgtggg cgatcggatt
      241 ttcggtgaac aggtaatccc gaagttcctt gtcgagcgcc ggatcccgac gccgaatcca
      301 ctccagcacc atcgccgcat gctctttttc ctcgtcgcgg ttgtgcgcca ggatggccgc
      361 caactcctta tccttgcagg cgtcgacccg ctggttgtac cagtcgaccg cctccagctc
      421 ttccatcaac gaggtgatcg cacggtgcat gtcgcgggtt tcgtcgctca gttcctcgat
      481 cggttcgtga tagccttcgt ttgccatgtg ttgctcctgc ggagtgagaa atcgtcgccc
      541 aatattagcc attatatcgg tgccggtcga ctgctaaccc ggtataatcg gtgcccggga
      601 gttcggcatc ccgtttgccg cggcgatgcg gcgaatcatg ccggcgacgg cgcagacagg
      661 tgaaccgaat aatctactat cggcagggca ggcaatggag ttcgacgaac tggagcccgc
      721 ggcggaccag gcgctgcaaa ccatcagcaa cgcgctgacg cacccgggta cttataccca
      781 gctgggtatc atcctcggta tctacgcggt cgcgttcatc gtcgccaacc gcctgcgcag
      841 ggtcgcgggc catttcaaca tcagcccgca acaggcggag ggccagccgc tgaagctgtt
      901 cgtcgtgcgg ctgatgaacc tgatcttccc gctgctggcg atcctgctgc tgcgggtatt
      961 cgtcgaaatc agcgaaaccc tgctggcgca gggctggctg atggaggccg cgctgacgat
     1021 cgcgatcgtg ttgcttttca actcgatcat cgacgatttc gtgcgccatc cgctggtgcg
     1081 gggtgtgctg cgctatctcg gcaacccgct gctcatcctg catctgctcg gggttctcga
     1141 cggcctgatc gaaatcctcg aatcgatctc gatctcgatc ggcaatatcg aaatttccgc
     1201 ctatggcatc gcgcgcgtcg cgatctttgg cacgctgctg ttctggatcg gccgtttttc
     1261 gaatcgcacc ggccgcgaac tgatcaatcg ccagtccaac ctcgattccc gcacccgcga
     1321 ggtcgccggc aagctgctcg agatctcggt attttttgtg atcttcctgc tgctgctgca
     1381 ggtcatgggc atcaacctga ccgcactcgc ggttttcggc ggcgcgatcg gtgttggtct
     1441 cggtttcggc ctgcaggcga tcgcatcgaa ttttatttcc ggcatcatca tcctgctcga
     1501 ccgctcgctg tcggtgggcg actacatcga gttcgacgag cagcaccgcg gcgtggtgcg
     1561 ggagttgaac atgcgctcga ccacgctgga gacctatgac ggctgcgaca tcatggtgcc
     1621 taacgagaag ttcatcgtcg aaaccttcat caactggacg cacaaggaca agcgccagcg
     1681 ctaccgggtc gatttctcgg tcgcctacga cagcgatatt cacaagctgg tggatatcgt
     1741 caggaaggtc gtagccagtc acccgcaggt gatcagcggt gccgaagtgg cggcagagga
     1801 gcagccggat tgcgagatcg acagtttcgg cgattccggt atcaacatgt tcgtcgagtt
     1861 ctggatgatc ggcatcgacg acggcaagaa ccgcgttggc ggcgacctgt tgctgatgat
     1921 tctcgatgcg ctgcgcgaac acgaattcga aatcccgttc ccgcaacgcg aggttcgcgt
     1981 gctgaatccg gctttttctt tcaacgcccc gaaacgcgtc gatccgaaat agcccggcct
     2041 gcggttcagt agcctggtcc gagcggcatc aggtgaccga ccttgatcgc cacgctgaag
     2101 tgcttgtgca tcatgaaacc gccgacgtcg acaccgccgt cgctctcgat gacctgcacg
     2161 atgcatccat aggaaaagtg atgggtgtag ccctgctttt cgtcgaccac cgagaaatga
     2221 tcgtcttcca ccgcggtgat gatcgccggc tcgaagcgaa acgcgttttc gatattgata
     2281 ccgacttcgc agttcaggta tttctcgagg atggttttca tctgtactgc cctctaaagc
     2341 cttgcgacta gcataacctg aagccgattg gcctgctttt gacacaggac aaggagaaat
     2401 accttgagcc gttcataccc cgttaagttt gtagttccca taatcgaaca aaatcacgcc
     2461 tagaggtaaa tccatgaacc aaatttcccg cagaaaaatg ctgggactgt cggctgccgc
     2521 gctgatcgtc ggcccggcac tggcgatgaa cagccgttcc tcgcttgccg cggagctgcc
     2581 caagctcgat cccaatgacc cgcaggcgaa aggtctcagc tatgtacaca agtcggcaaa
     2641 ggccgacaac tattgcggca actgccagct gtacaccggc gatcccgccg cctcgtgggg
     2701 gccctgccag atttttcccg gcaagcaggt tgccagcgcc ggctggtgca gcgcctgggt
     2761 caagaaagcc ggctagcggc aagccgcggc tcgcccgcag cgtgccgctt gaggccggct
     2821 gcgggcgagc aaacacattt cgcacatggc aagcgaggat tgccggcccg gtcacacggt
     2881 tcccgcgcgg gggcgaatta caccacttta aaggaattac gacgcgctgc tagatcgcgg
     2941 gtccgagttc gagcttgctg ccgtcgctga aacgcgacaa tcgaaagcgc tgcagatcgt
     3001 gacccgcgga attgccctgt atcaggtcgg ccatcacgcg gccggcggcc ggtccgatgc
     3061 cgaagccgtg gccgctgaag ccggtcgaga tccataagcc atcgaggttt gccgcgcggt
     3121 cgaggaccgg cacgacgtca ggcagagcgt cgatcattcc cgcccaggat tccgccagtt
     3181 cgaccccggc gaatgccggc acgcgatcgg cgagcagcgc cttgatgcgc gctacgacct
     3241 tgtcgcgtgg ttgcgggtcg aggctgcgat gacggctgaa attgccgtcg acgaccagcc
     3301 ccggcgggcg caggctcagg gtgcccagcg aggtgcgcag cagcggcagg aagtcccgcg
     3361 cgaaagccag gtgcgcgggc gatggcagga tctccagatg tcccttgatc gcgatcgtgt
     3421 agccgccgtc gaggcggcgc cgaaacgaaa cctcgctacc cgacgcgttg ccgtcgtaga
     3481 tcgatggcat cgctgcggtg cgcgcgacac tcgccttgac cgtgagctgc ggcaggcgaa
     3541 tcccgcagga cttgagtaaa aacgtcgacc aggcgcccgc cgcgatcagt accgtcgaag
     3601 tctcgatgtc gccatgctcg gtggccacac tcgacacgcg gccgttttgc gtatcgatgc
     3661 cgttgaccgc gcagttctcg atgatcggca cgccgcgcgc gcgtagcgtc cgcgccatgg
     3721 ctggcaccgc cagcgagggt tcggcgcgcg catcgtcggg cgtgtggagt gcgcttcgcc
     3781 agcgcggcgg gcagtgcgga aacacggctt cgagctgctt gcggtgcagg cggcgcgtgt
     3841 cgagaccgta atcgggcgca aaattcatga agtggtcgta gttggcgagg tcgcgatcgc
     3901 tttcgcacag gtagagactg ccgctgcgac ggaagccgat gtcggtgtcg acatcgtcgg
     3961 cgatttcctg ccagaggcgc aggctttcga tgacaatcgg taactcggcc tggtctcgtc
     4021 ccatttgccg acaccagccc cagttgcggc tcgactgctc gccggcaacc acgccttttt
     4081 cgcaaaccgc aacctcgatg ccggcgcgct gcaggaacca ggccgcggcg atgccggcga
     4141 tgccgccacc gacgacgatg acctctgcgg cggtgggcaa cttcgggctg gagtcattca
     4201 tgggcaggtc gcgagtgaca gatttcgggg ccaatgatac gatgatttac gcgtgcgcgc
     4261 gtacaaaatc tcgaaccgcg aggaagcccg aaccgcgtgg aagccattcc ccagaccagt
     4321 agtttcggcg ccgaggtgac ggggctggat ctgtcgcagc cgctcgatgc tcggcgcttc
     4381 gagcggctca agcggatgtt gctcgattat caggtgctgt tgtttcgcga ccagcaaatt
     4441 tcgccgcgcc attaccagga ttttgcacgg cgcttcggcc gcttgcagga atacctgttc
     4501 gccgagggtc tcgacggctt cccgtacatc accgaaattg tcaagaccga gaacgaaacc
     4561 ggcggattcg gcgatttctg gcactcggat tcggcctacc tcgaaacgcc gccctcgatc
     4621 accatgctgt atgcccggca ggtaccgccg catggcgggg atacgctgtt caaggacatg
     4681 tatgccgtct acgacgaa
//
LOCUS       DATCZV010000369         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_612508, whole genome shotgun sequence.
ACCESSION   DATCZV010000369 DATCZV010000000
VERSION     DATCZV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612508"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1139)
                     /locus_tag="VIW27_08265"
     CDS             complement(381..1139)
                     /locus_tag="VIW27_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY5739706.1"
                     /translation="MCLILFAYRQRDDYPLILIANRDEYYARPTRYAHWWPKAAVLAG
                     RDLEAGGSWLGISRGGRLAAVTNVREAGGMRAGKRSRGELTRDFLCDSVAATAYLETI
                     AARAQDYAGFNLLLGDLDELWFYTNRGNTAQRIAPGVYGISNGSFDEPWPKLSSGKAE
                     LAGLLDGDLDSSKLMAILTDHRTAEDHLLPSTGVPLDVERLLSSRFIRSPDYGTRACT
                     VLLIGADGRVDFSEQNFGIQGTPGELVQETFTIE"
     gene            complement(1156..2430)
                     /gene="sbmA"
                     /locus_tag="VIW27_08270"
     CDS             complement(1156..2430)
                     /gene="sbmA"
                     /locus_tag="VIW27_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HEY5739707.1"
                     /translation="MFKSFFPNPKLFFLSVLVYTGLCVAVWYGFREQMGALLGFDLSE
                     GAPIIGLGHFVTDEFLLFYLYYIACTALFAGAWFRLARHPWQWWSIVGSATILFSSYF
                     SVQVSVAINNWRRPFFDAVQRMLQRIVDGTSAEELPPLPAETIATNKAELYDLIVIFA
                     EIAFLAIFVYVMTRFLTSHFVFRWRTAMNDYYTAQWATVRNIEGASQRIQEDTMRFAD
                     IMENLGVSIVEAVMTLFAFLPVLWALSAYVSELPLVGAIPQPLFWAALVWAIFGTGFL
                     ALVGIKLPGLYFKNQRVEAAFRKELVYGEDDENRAQPPALKDLFANVRRNYFRIYFHY
                     MYFNVARMLYLQADAIFVYVLLVPTIAVGAITFGILQQILTAFNQVSNSFQYLVNSWT
                     TIIELLSIYKRLKSFEAAIRNEPLPAIDQYAT"
     gene            complement(2525..>3510)
                     /gene="hemH"
                     /locus_tag="VIW27_08275"
     CDS             complement(2525..>3510)
                     /gene="hemH"
                     /locus_tag="VIW27_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5739708.1"
                     /translation="SGPTSRPAANTAVLLTNLGTPDRLERAAVKRFLREFLSDPLVVR
                     LPRAFWLPLLHGIILPLRSGRTLEAYSRVWSEQGSPLLAYASRQQAALQRLLADSARV
                     ELAMRYGNPSFESVLRPLRDAGVDRLVVLPLYPQYSVTTTATSYKHLMANLRALDWHP
                     ALEFIGYYPDHPAYIDALARSVDEHHSGEPCHLLMSFHGLPQANVDRGDPYQRQCEQT
                     ARLLAARLGLDASDWSIGYQSRFGKQTWIQPYTSDVLRDLVARGVTAVDVICPGFSAD
                     CLETLDEIEVEYRKEFLELGGETFSYIPALNDSDAHIEMMRQLVEPCLSDD"
BASE COUNT          731 a         1032 c         1099 g          648 t
ORIGIN      
        1 cccacctttc aattcccgaa caatcgcggc aaatttgctc tgaccccacc tctcaactcc
       61 cgaaaccacc ggcaaatttg ctccgacccc gaatgtccaa cctcgggaaa cgctttgccg
      121 attggaatcg caactttggg agacttgggg acagtccagc tgtcgccatg cttcgcctgt
      181 tgtctccgtc ggtaccgaca gctgccctgt ccccctcgaa gagagcaaag tatctcaagt
      241 tctcctcgat accggccgaa ggtcgcgccg aacagttcct tgcagttctt tgggagggca
      301 ttcgattttg gataattttt acaaacccgg gaaagcttca atctattcgt ataaccccga
      361 tctttcccgc cgccgaaaaa ctattcgatg gtaaaggtct cctgaaccag ttcgccgggc
      421 gtgccctgta tgccgaaatt ctgttcgctg aagtcgactc gcccgtcggc gccgatcaac
      481 agcacagtgc aggcccgcgt gccgtaatcc ggcgaacgaa tgaagcggct ggacagcagg
      541 cgttcgacat cgagcggcac gccggtgctc ggcagcaagt gatcctcggc ggtgcgatga
      601 tcggtcagga ttgccatcag cttgctgcta tccaggtcac catccagcag gccggcgagt
      661 tccgccttgc cggaactcag ctttggccag ggctcgtcga aactgccgtt gctgataccg
      721 tagacgccgg gcgcgatgcg ctgcgccgta ttgccgcggt tggtgtaaaa ccagagctcg
      781 tcgagatcgc cgagcaacag gttgaatccg gcgtagtcct gcgcccgcgc ggcaatggtt
      841 tcgaggtaag cggtcgcggc gaccgaatca cagaggaagt cgcgcgtcag ttcgccgcgc
      901 gaacgcttgc ccgcgcgcat gccgccggct tcgcgcacgt tggtgaccgc tgccaaccgg
      961 ccgccgcggc tgatgccgag ccagctgccg ccggcctcga gatcgcgccc ggcgagcacc
     1021 gccgctttcg gccaccagtg agcgtatcgc gtcggccgcg cgtagtactc gtcacgattg
     1081 gcgatcagga ttagcggata gtcatcgcgc tggcggtagg cgaacaggat caggcacatg
     1141 tgaatccgct atgcatcagg tcgcatactg gtcgatggct ggcagcggct cgttgcggat
     1201 tgccgcctcg aaagacttca ggcgcttgta aatcgacagc aattcgatga tcgtggtcca
     1261 ggaattgacc aggtactgga acgaattgct gacctggttg aaggcggtca ggatctgctg
     1321 caggataccg aaggtgatcg caccgaccgc gatcgtcggc accagcagca cgtagacgaa
     1381 gatcgcgtcg gcctgcaggt agagcatgcg cgcgacgttg aaatacatgt agtgaaagta
     1441 gatgcggaag tagttgcggc gaacgttggc gaacaagtcc ttcagcgcgg gcggttgcgc
     1501 gcggttctcg tcgtcttcgc cgtagaccag ctccttgcga aaagcggcct cgacccgttg
     1561 gttcttgaaa tacaggccgg gcagctttat tcctaccagg gcaaggaaac cggtaccgaa
     1621 tatagcccac accagcgccg cccagaacag gggttgcggt atcgcaccga ccagcggcaa
     1681 ttcgctaacg taggccgaca gggcccacaa taccggcagg aaggcgaaca gggtcattac
     1741 cgcttcgacg atcgaaacgc cgaggttttc catgatgtct gcaaagcgca tggtgtcttc
     1801 ctggatacgc tgcgaggcgc cctcgatgtt acgcacggtg gcccactggg cggtatagta
     1861 atcgttcatc gcggtacgcc agcgaaatac gaaatggctg gtcaggaaac gggtcatgac
     1921 gtaaacgaag atggccagaa aggcgatctc cgcgaagatt acgatcaggt cgtacaattc
     1981 cgccttgttg gtggcgatgg tctcggcggg caacggcggc agctcttcgg ctgacgtgcc
     2041 gtcgacgata cgctgcagca tgcgttgcac cgcgtcgaag aacggccggc gccagttgtt
     2101 gatagccacc gatacctgca cggagaagta gctcgaaaac aggatcgtcg ccgaaccgac
     2161 gatcgaccac cactgccagg ggtgcctggc gagcctgaac caggccccgg cgaacagcgc
     2221 ggtacaggcg atgtaataca ggtaaaacag caggaactcg tcggtaacga aatgtccgag
     2281 gccgatgatc ggcgcccctt ccgagaggtc gaatccgagc aaggcgccca tctgttcacg
     2341 aaaaccgtac cacactgcga cgcatagccc cgtgtaaacc aggaccgaaa ggaagaacag
     2401 tttgggattg ggaaaaaagg acttgaacat ggcttatgac tcggtaattg ccggtatcag
     2461 cggcttgttg acggctacgg atatgggtac cgaaatacga aaatcattca aacccggtct
     2521 gacctcaatc gtcggacagg cagggctcga ccagctggcg catcatctcg atgtgcgcat
     2581 cgctgtcgtt gagcgccgga atatagctga aagtctcgcc gcccagctcg aggaattcct
     2641 tgcggtattc gacctcgatt tcatcgaggg tttcgaggca atcggccgag aagccggggc
     2701 agatgacgtc gacggcggtg acgccgcgtg ccaccaggtc ccgcaacacg tccgaggtat
     2761 agggctgaat ccaggtctgc ttgccaaaac gcgactggta gccgatcgac cagtccgagg
     2821 cgtcgagtcc gagccttgcg gccagcagcc gcgcggtctg ctcgcattgc cgctggtaag
     2881 ggtccccgcg gtcgacgttg gcctgcggca agccgtgaaa cgacatcagc aagtggcacg
     2941 gttcgccgga gtggtgttca tcgaccgagc gcgccagcgc gtcgatgtaa gccgggtgat
     3001 cgggataata gccgatgaat tcgagtgccg ggtgccagtc gagcgcgcgc aggttcgcca
     3061 tcaggtgctt gtagctggtg gcggtggtgg taaccgagta ttgcgggtag agcggcaaca
     3121 ccaccagccg gtcgacgccg gcgtcgcgca gcggcctgag caccgattcg aacgacgggt
     3181 tgccgtagcg cattgccaac tcgacgcgcg cgctgtccgc cagcagtcgc tgcagcgcgg
     3241 cctgctgccg actggcgtag gccagtagcg gcgagccctg ctcactccag acgcgactgt
     3301 aggcctcgag cgtcctgccg ctgcgtaacg gcagaattat gccgtgtaac agcggcagcc
     3361 agaaagcgcg cggcaggcgc accaccagcg ggtcggagag gaattcgcgt agaaatcgct
     3421 tgaccgcggc gcgttcaagg cgatccgggg tgccgagatt ggtcaggagt accgcggtgt
     3481 tggcggctgg tcggctggtc ggcccggatt
//
LOCUS       DATCZV010000370         5129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_242186, whole genome shotgun sequence.
ACCESSION   DATCZV010000370 DATCZV010000000
VERSION     DATCZV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242186"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /gene="sucC"
                     /locus_tag="VIW27_08280"
     CDS             complement(<1..1239)
                     /gene="sucC"
                     /locus_tag="VIW27_08280"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5739709.1"
                     /translation="MYIHEYQAKQILRDNGIPSPPGIVTTTVEEAVQAARDLGGQSWA
                     VKAQVYSGGRGAGRFQGQDSTQGGVRIVDSLDAVRRSSEEMLGQTLITEQTGSVGRKV
                     ERLYIETGCDIERELYLAMLVDRASNQLLLVASANGGQNIEKVAAEDPDAILRVPIDL
                     LDGPSREEIGNALAAFELPEAQAAQVLDVVEAMFRLFKSLDCSLIEINPLAITHSGEV
                     LALDSVMTFDNNALFRHPEIRDLRDEKQLPPGMLRAYVHGINYVKLDGDIGYLANGAG
                     LALATLDAIVYAGGKPANFLDVPPVVQQSIVRDAFKLLTSDPDVKAILVNIFGGGIMR
                     CDTVADAILTALVDEPLKVPLVVRLMGVDAELGLSRLKNSGHGILFANSLTEVANLAV
                     DAAQKGRMDGRKSWWDRANSL"
     gene            complement(1253..2239)
                     /locus_tag="VIW27_08285"
     CDS             complement(1253..2239)
                     /locus_tag="VIW27_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY5739710.1"
                     /translation="MKAITLREFGGPEVMQLGEVDKPSPGDGQLLVKVVNTSVNRPDV
                     IQRMGNYPPPPGDSEILGLEIAGVVEACGPGTERFMPGDPVFGLVGGGGYAEYALARE
                     DHCMPIPDALEFEQAACICETYITAYLNLFLLGEAMNKPAVLIHGGGGGVATSAIQLC
                     RALTPDTRIFVTASTGKLERVREQGAHQVIDYREQDFAEEIRNLTDRRGVDVILDHIG
                     ADYLERNMKSLALAGTLMLIGVMGGIKAELNLATMMVKRQRIIGSVLRSRPPAEKAAI
                     IEKFEADVLPLFASAKIEPLIDARFPLADAAQAHRLMEKSGHFGKIVLSNQG"
     gene            complement(2311..3900)
                     /gene="ggt"
                     /locus_tag="VIW27_08290"
     CDS             complement(2311..3900)
                     /gene="ggt"
                     /locus_tag="VIW27_08290"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5739711.1"
                     /translation="MRNHQLPGRSVVMAADAMAATSQPLATEAAIRVLREGGNALDAA
                     IAASGVLSVVETYSSGIGGDCFILYHEAASGKLHALNGSGRAPAAATAEAIRARGFSS
                     MPEQDILAVTVPGAVDAWCEAVEKFGRLEFAQLLEPAIHYAEQGYPVSPVIAHNWNKT
                     ADLLRQTPEASKAYLVDGEAPAAGDMHRQPDLARSLRLIAERGRAGFYQGEIAEEIVR
                     YSDSMGGLLTLDDLAEHHCEWVEPISSNYRGYEVFEIPPNGQGITTLMALNILAQTQV
                     AQYPHLGADHVHLLSEAFTLAMAERDRYVADLNFRPLPVAEMLSNEFARTQFERIDMA
                     RALPQPVESALPNHRDTVYLTVVDRDRNVCSFINSVFYSWGCGLVAGSTGINLGNRGS
                     GFVLDDGHFNQIEPRKRPLNTIIPAMAYRDDKPVLSFGVMGGQFQAMGQTYVLSNWID
                     YGMDIQESIDAARFFLYGGALDVETGVPQATREGLAARGHRVIEDDGPHGGGQGIAID
                     WQRGVLHGGSDPRKDGSAMGY"
     gene            4150..>5129
                     /locus_tag="VIW27_08295"
     CDS             4150..>5129
                     /locus_tag="VIW27_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEY5739712.1"
                     /translation="MTDNEPEENPEAPRGRYRWLAFAAAAFGFAATWWAWPLDDANQA
                     LVAATLVAVVVLWVSEVVPLYVSGLIGSFVLLAFGEFSAAQIFAPYFDPVIALFLGGF
                     VLARGMQKYALDHRIAHEILKRIGDRPSIFVLGLMGVTAFLSMWMSNTASAAIMIPIS
                     IIVLGENHLFHRQSRYARGVVLSIAYAATIGGIGSLVGSPPNAIAARYLGERGVELNF
                     LEWMLKTLPFVVLALLFTWLLLLLFNKPEIERIRFTYRPRPADRAQKLVLLIFAVTVL
                     GWLSTRLTGISAYAIALIPVIGLFALRLLDDRDLAAISWPTLLLFGGGLSL"
BASE COUNT          835 a         1634 c         1672 g          988 t
ORIGIN      
        1 cagcgagttg gcgcggtccc accatgactt gcggccgtcc atgcggccct tttgcgccgc
       61 gtcgacggcg aggttggcga cctcggtcag gctgttggcg aacaggatgc cgtggccgga
      121 gttctttagc cggctgaggc cgagctcggc gtcgacgccc atcagccgca ccaccagcgg
      181 aaccttcagc ggttcgtcga cgagcgcggt gaggattgca tcggcgaccg tgtcgcagcg
      241 catgatgccg ccgccgaaga tattgacgag gatcgccttg acgtcgggat cggaggtcag
      301 cagcttgaac gcatcgcgca cgatcgactg ctgcaccact ggcggcacgt cgagaaagtt
      361 ggccggcttg ccgccggcgt agacgatcgc gtccagcgtt gccagcgcga gcccggcgcc
      421 gttagccagg tagccgatat caccgtcgag cttgacgtag ttgatgccgt ggacgtaggc
      481 gcgcagcatg ccgggcggca actgtttttc gtcgcgcagg tcgcgaatct ccgggtggcg
      541 gaacagcgcg ttgttgtcga aggtcatcac cgagtcgaga gccagtacct cgccactgtg
      601 agtgattgcc agtggattga tttcgatcag cgagcagtcc agcgacttaa acagccgaaa
      661 catcgcttcg acgacgtcga gcacctgcgc ggcctgtgcc tcgggcagtt cgaaggcggc
      721 gagggcgttg ccgatctcct cgcgggaagg gccatcgagc aggtcgatcg gtactcgcag
      781 aatggcatcc ggatcctcgg cggcgacctt ttcgatgttc tgtcctccat tcgccgaggc
      841 taccagcagc agctggttgc tggcgcgatc gaccagcatc gccaggtaaa gctcgcgttc
      901 gatgtcgcaa ccggtctcga tataaagacg ctcgaccttg cgcccgacgc tgccggtctg
      961 ctcggtgatc agcgtctggc cgagcatctc ctcgctgctg cgcctgactg cgtcgaggga
     1021 atcgacgata cgcacgccgc cctgggtcga atcctggccc tgaaagcggc cggcgccgcg
     1081 accgcccgag taaacctgcg ccttgacggc ccagctttgc ccacccaggt cgcgcgccgc
     1141 ctgcacggct tcctcgacgg tggttgtgac gatgcccggc ggcgacggaa tgccgttgtc
     1201 gcgaagaatc tgcttggcct ggtattcgtg gatatacatg cgttcccccg gcttacccct
     1261 ggttcgacag cacgatcttg ccgaagtgcc cgctcttttc catcaggcgg tgcgcctgtg
     1321 ccgcgtcggc gagcggaaag cgcgcgtcga tcaagggctc gattttagcg gaagcgaaca
     1381 gcggtagcac gtcggcttcg aatttttcga tgatcgcggc tttttcggcc ggcggccgcg
     1441 agcgcagcac cgaaccgatg atgcgctgtc gcttgaccat catcgtcgcc aggttgagtt
     1501 ccgccttgat gccgcccatc acgccgatca gcatcagcgt gccggccagc gccagcgatt
     1561 tcatgttgcg ctcgaggtag tcggcgccga tatggtcgag gattacgtcg acaccgcgcc
     1621 ggtcggtgag gttgcggatt tcctcggcga aatcctgctc gcggtaatcg atgacctgat
     1681 gcgcaccctg ctcgcgcacc cgctcgagct tgccggtcga ggcggtgacg aagatgcggg
     1741 tgtccggcgt caacgcccgg cacagttgaa tggccgaggt cgcgactccg ccaccgccgc
     1801 cgtgaatcag cactgccggc ttgttcatcg cctcgccgag caggaacagg ttgaggtagg
     1861 cggtaatgta ggtttcgcag atgcacgctg cctgctcgaa ttcgagcgcg tcggggatcg
     1921 gcatgcaatg gtcttcgcgc gccagcgcgt actcggcata accgccgcca ccgaccagtc
     1981 cgaagaccgg gtcgccgggc atgaagcgct cggtacccgg gccgcaggct tcgaccacgc
     2041 cggcgatttc caggccgaga atctccgaat cgcccggcgg gggcgggtaa ttgcccatcc
     2101 gctggatgac gtccggccgg ttgaccgaag tgttgacgac cttgacgagc agctgcccgt
     2161 cacccggcga cggcttgtcg acttcgccca attgcatcac ttcggggccg ccgaactcgc
     2221 gcagagtaat ggctttcatg cgttcattcc ccgatttcga aatctggtat acagtatacc
     2281 agtatagtgg tcaggtaaag catacagccg ttagtatccc attgccgagc cgtctttgcg
     2341 agggtccgag ccgccgtgca gtacgccgcg ctgccagtcg atggcgattc cctggccgcc
     2401 gccgtgcggg ccgtcgtcct cgatcacgcg atgtcccctg gccgcgaggc cttcgcgcgt
     2461 cgcctgcggt acgccagtct cgacgtcgag tgcgccgccg tagaggaaga agcgggcggc
     2521 gtcgatcgat tcctggatat ccatgccgta atcgatccag ttgctgagta cgtaggtctg
     2581 gcccatcgcc tggaattgcc cacccatgac accgaaagac agcaccggtt tgtcgtcacg
     2641 gtaggccatc gccggaataa tcgtgttcag cggtcgcttg cgcggttcga tctggttgaa
     2701 gtgcccgtcg tcgagcacga agccggagcc gcgattgccc aggttgatgc cggtcgagcc
     2761 ggccaccaga ccgcaacccc aggaatagaa cacgctgttg atgaacgagc agacgttgcg
     2821 gtcgcggtcg accacggtca ggtaaacggt atcgcggtga ttcggcagtg ccgactcgac
     2881 cggctgcggc agcgcgcgtg ccatgtcgat gcgctcgaac tgcgtgcgtg cgaactcgtt
     2941 cgacagcatc tcggcgaccg gcagcgggcg aaagttcagg tcggcgacat agcggtcgcg
     3001 ctcggccatc gccagcgtaa aggcctctga cagcaggtgc acgtgatcgg cgccgagatg
     3061 cgggtattgc gcgacctggg tctgcgcgag tatgttgagc gccatcagcg tggtgatacc
     3121 ctggccgttg ggcggaatct cgaacacctc gtagccgcgg taattgctgc tgatcggctc
     3181 cacccactcg caatgatgct cggcgagatc gtcgagtgtc agcaggccgc ccatggaatc
     3241 gctgtagcgg acgatctcct cggcgatctc gccctggtaa aagcccgcgc gcccgcgctc
     3301 ggcgatcaac cgcaggctgc gcgcgagatc cggctggcgg tgcatgtcgc cggccgccgg
     3361 cgcctcgccg tcgaccaggt aagccttgct ggcttccggg gtctgccgca gcagatccgc
     3421 ggttttattc cagttgtgcg cgatcaccgg cgataccggg taaccctgct cggcgtagtg
     3481 gatggccggc tcgagcaatt gcgcgaattc gagtctgccg aacttttcca ccgcctcgca
     3541 ccaggcgtcg accgcgccgg gcacggtgac cgcgaggatg tcctgctccg gcatgctgct
     3601 gaagccgcgc gcgcgaatcg cctcggcggt ggccgcggcc ggcgcgcgcc cgctgccgtt
     3661 cagcgcgtgc agcttgccac tggcggcctc atggtagagg atgaagcaat cgccgccgat
     3721 acccgacgaa taggtttcga ccaccgacag tacgccgctg gcggcgatcg ccgcgtcgag
     3781 cgcgttgccg ccctcgcgca agacgcgaat cgcggcttcg gtggccagcg gctgactggt
     3841 ggcggccatt gcgtcggcgg ccatgacgac cgaacggccg ggtagctggt ggttgcgcat
     3901 cgaggttccc gagttcgaag gttaaagagg gcagtatact atctcgagcg ccagaaacct
     3961 gccccgtcat cgcccggttt gcgtatttaa ttgatgcagg tccgggaaac gggcgccgcg
     4021 gcagctatgc tgaggtcaaa ggctgtaccc attcccattt ttcggatcaa gattgccgaa
     4081 ttaaaatgac tctgatccca tttacggaag ctgccggcgg ggtaccgacg gaaatgtcga
     4141 ggcggatcga tgaccgataa cgaacccgag gaaaaccctg aggccccgcg cggccgctac
     4201 cgttggctgg cattcgccgc ggcggcgttc gggtttgcgg caacctggtg ggcgtggccg
     4261 ctggacgacg ccaaccaggc gctggtggcg gcgacgctgg tggcagtcgt ggtgctgtgg
     4321 gtcagcgagg tcgtgccgct gtacgtctcc gggctgatcg gttccttcgt gctgctggcg
     4381 ttcggcgagt tttccgccgc gcagatcttt gcaccctact tcgacccggt catcgcgctt
     4441 tttctcggcg gcttcgtgct cgcgcgcggc atgcaaaaat acgcgctcga ccatcgcatc
     4501 gcccacgaga tcctgaagcg catcggtgac cggccgtcga tcttcgtgct cgggctgatg
     4561 ggggttaccg cgttcctgtc gatgtggatg tccaacaccg cgtcggcggc gatcatgatt
     4621 ccgatctcga tcatcgtgct cggcgagaac cacctgtttc accgccagtc gcgctacgcc
     4681 cgcggcgtgg tgctgtcgat cgcctacgcg gccaccatcg gtggcatcgg ctcgctggtc
     4741 ggcagcccgc cgaacgcgat cgccgcgcgt tatctcggcg agcgcggcgt cgaactcaac
     4801 tttctcgaat ggatgctgaa aacgctgcca ttcgtggtcc tcgcactgct gtttacctgg
     4861 ttgttgttgc tgcttttcaa caagcccgag atcgagcgga ttcgctttac ctaccgcccg
     4921 cggccggccg accgggcgca gaaactggtg ctgctgatct tcgcggtcac cgtgctcggc
     4981 tggctcagca cgcggctgac cgggatatcc gcctacgcga tcgcgctgat cccggtgatc
     5041 ggcctgttcg cgctgcgcct gctcgacgac cgcgacctgg cggcgatctc atggccgacg
     5101 ctgctgctgt tcggcggcgg cctgagcct
//
LOCUS       DATCZV010000371         4167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_136182, whole genome shotgun sequence.
ACCESSION   DATCZV010000371 DATCZV010000000
VERSION     DATCZV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136182"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VIW27_08300"
     CDS             complement(<1..629)
                     /locus_tag="VIW27_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5739713.1"
                     /translation="MRAVPRRTKIVATLGPATESDAAIDGLIKAGVDVVRLNFSHGTV
                     DEHKVRAERIRRISSENNRHLAILADLQGPKIRLRKFKKGAVDLEIGDDFCLDTECAD
                     GDGDRQRVGVTYGKLPDDVKGGDILVLDDGRILLSVESVDGPRIHCKVAVGGVLSDNK
                     GVNLKGGGLTAEVLTDKDRADIKTAALIDADYIAVSFPRNAADITQARRL"
     gene            complement(610..1809)
                     /locus_tag="VIW27_08305"
     CDS             complement(610..1809)
                     /locus_tag="VIW27_08305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY5739714.1"
                     /translation="MPVLQMTGLELAGKRVLIRQDLNVPLEDGRVASDQRIQASLPAI
                     RHCIEAGARLMIMSHLGRPEEGKPESQYSLRPVADYLTAALGQPVALLDDYLEHAPQL
                     ADGGVALLENVRFNVGEKNDDEALARRYAALCDIFVMDAFGTAHRAQASTHGVARFAP
                     VACAGPLLAAELDALGKALHAPARPVVAIVGGAKVSTKLTVLESLSSIVDQLIPGGGI
                     ANTFIAAAGHEVGKSLVEEELIPEAERLMVQAKAEGREIPVPVDVVCGKEFSATAAAE
                     TKSVDAVEQDDMIFDIGPQTAERFAEMFRNAATIVWNGPVGVFEFDQFGGGTRRLAEA
                     IADSPAFSIAGGGDTLAAVDKYGIADRISYISTGGGAFLEFLEGKQLPAVTVLEQRYA
                     DHEGSST"
     gene            complement(1818..2813)
                     /gene="gap"
                     /locus_tag="VIW27_08310"
     CDS             complement(1818..2813)
                     /gene="gap"
                     /locus_tag="VIW27_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY5739715.1"
                     /translation="MTVRVGINGFGRIGRMVFRAARDFDDVEIVGINDLLEPDYLAYM
                     LKYDSVHGRFAGEVAIEDGALIVDGSRIRLTAERDPAALNWGDLDVDVVVESTGLFLT
                     AETAQKHIDAGASKVIMSAPSKDATPMFVFGVNHDSYAGESIISNASCTTNCLAPIAK
                     VVDDNFGIKRGLMTTVHAVTASQKTVDGPSAKDWRGGRGILDNIIPSSTGAAKAVGKV
                     LPQLNGKLTGMAFRVPTPDVSVVDLTVELKNGASYEDICAAMKAAAEGPMKGVLAYTE
                     DKVVSTDFRGESCTSIFDAEAGIQMDETFVKLVSWYDNEWGYSCKVVEMARVITG"
     gene            complement(2848..>4167)
                     /locus_tag="VIW27_08315"
     CDS             complement(2848..>4167)
                     /locus_tag="VIW27_08315"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5739716.1"
                     /translation="ARAISARPSLICCKTVIGFGSPNKAGSHDCHGAPLGEEEIAATR
                     AALEWEHDAFVIPEEIYQGWDHRDKGQSREDAWQQKFDEYRQAHPELAAEFERRINGE
                     FPAHWQEEADAFIAEVVEAGEDKATRQASLATIEAFAPLLPELFGGSADLGGSNGTEW
                     SGYKPMRAESPDANYINYGVREFGMSAIINGIVLHGGLLPFGATFLTFSDYARNALRM
                     AALMKVQSIFVYTHDSIGLGEDGPTHQAIEHIPSLRLIPGMQVWRPCDTVETAVCWKA
                     AIEHRDGPSCLVFSRQSLKFQPRDAEQIAAIARGGYVLRDCEGTPDAIIIATGSEVEL
                     AVAAADSEELEGCRIRVVSMPSTTMFDSQSREYRDSVLPPEVTARISVEAASTLTWHK
                     YVGTHGIIIGIDSFGESAPAKELFAYFGFTVGKVVEAVKKLTGDDEA"
BASE COUNT          745 a         1363 c         1283 g          776 t
ORIGIN      
        1 agccggcgcg cctgggtaat atccgcggca ttgcggggaa aggacaccgc gatgtagtcg
       61 gcatcgatca gcgccgcggt cttgatatcg gcgcggtcct tgtcggtgag cacctcggcg
      121 gtcagcccgc cgcccttgag attgacgccc ttgttgtcag acagcacgcc gccgaccgcg
      181 accttgcaat gaatacgcgg gccatccacc gattcgaccg atagcaggat gcgtccgtcg
      241 tcgagtacca ggatatcgcc gcccttgacg tcgtcgggca gcttgccata ggtgacaccg
      301 acccgctgcc ggtcgccgtc gccgtcggca cactcggtgt cgaggcaaaa gtcatcgccg
      361 atctcgagat ccacggcgcc ttttttgaat tttctgaggc ggattttcgg gccctgaaga
      421 tcggcgagaa tcgccaggtg gcggttgttt tcgctgctga tacggcgaat tcgctcggcg
      481 cggaccttgt gttcgtcgac cgtgccatgc gagaaattga ggcgtacgac gtcgacaccg
      541 gccttgatca ggccatcgat cgcggcatcc gactcggtcg ccggacccag ggtggcgacg
      601 atcttggtcc tacgtggaac tgccctcatg atctgcatat ctctgctcca gcaccgtcac
      661 cgccggcaat tgcttgcctt cgagaaattc gagaaaggcg ccgccgccgg tggagatata
      721 tgaaattcgg tcggcgatcc catacttgtc gaccgcggcc agtgtatcac ccccgccggc
      781 gatcgagaac gcgggggaat cggcgatggc ttccgccagc cgccgggtac cgccaccgaa
      841 ctggtcaaat tcgaatacgc cgaccggccc gttccagacg attgtcgccg cgttacgaaa
      901 catctctgca aagcgctcgg cagtttgcgg cccgatatcg aaaatcatgt cgtcctgctc
      961 gaccgcgtcg acggacttgg tctcggccgc ggccgtggcg gaaaactcct tgccgcagac
     1021 cacgtccacc ggcaccggga tttcgcgacc ctcggctttg gcctgcacca tcagccgttc
     1081 ggcctccggg atcagctcct cttcgaccag cgatttgccg acctcgtggc cggccgcggc
     1141 gataaaggta ttggcgatgc cgccgcccgg gatcaattgg tcgacgatcg acgacagcga
     1201 ctcgagcacc gtcagcttgg tggatacctt ggcgccgccg acgatcgcca ccaccggccg
     1261 cgctggcgca tgcaatgcct tgccgagcgc atcgagctcg gccgccagca acgggccggc
     1321 gcaggccacg ggcgcgaagc gcgcgacccc gtgcgtcgac gcctgcgcgc ggtgcgcggt
     1381 gccgaaagca tccatgacga aaatatcgca aagcgcggcg tagcggcgcg cgagtgcctc
     1441 gtcgtcgttc ttctcaccga cgttgaagcg cacgttctcg agcagcgcga ccccgccgtc
     1501 ggcgagttgt ggcgcgtgct cgaggtaatc gtcgagcagc gcaaccggct ggccgagggc
     1561 agcggtcagg taatcggcga ccggccgcag cgagtactgg ctttcaggct tgccctcttc
     1621 ggggcggccg agatgggaca tgatcatcag ccgtgcgccg gcctcgatgc aatgacgaat
     1681 cgccggcagc gaggcctgaa tacgctgatc gctcgcgacc cggccgtcct cgagcggcac
     1741 gttcagatcc tggcggatca gcacgcgctt gccggccagc tcgaggccgg tcatttgcag
     1801 gacgggcatc ggaacgctca gccggtgatg acgcgtgcca tttcgacgac cttgcaggaa
     1861 taaccccact cgttgtcgta ccaggacacc agcttgacga aggtttcgtc catctggatg
     1921 ccggcctcgg cgtcgaagat cgaggtgcag gattcaccgc ggaaatcggt cgataccacc
     1981 ttgtcctcgg tataggccag cacgcccttc atcggaccct cggcagcggc cttcatcgcg
     2041 gcgcagatat cctcgtagct ggcgccgttt ttcaactcga cggtcagatc gaccaccgac
     2101 acgtcgggcg tcggtacgcg aaacgccatg ccggtcagct tgccgttcaa ttgcggcagc
     2161 accttgccga ccgccttggc ggcgccggtc gacgacggaa tgatgttatc gagaatgccg
     2221 cgcccgccgc gccagtcctt ggccgacgga ccgtcgacgg tcttttgcga tgcggtcacc
     2281 gcgtgcacgg tggtcatcag gccgcgcttg ataccaaaat tatcgtcgac gaccttggcg
     2341 atcggcgcca ggcagttggt ggtacacgag gcgttggaga taatcgattc gccggcgtag
     2401 gaatcgtggt tgacgccgaa cacgaacatc ggcgtcgcgt ccttcgacgg cgccgacatg
     2461 atgaccttgc tggcgcccgc gtcgatatgc ttttgcgcgg tctcggcggt caggaacagg
     2521 ccggtcgact cgaccacgac gtcaacatcg agatcgcccc agttcaaggc ggccggatcg
     2581 cgctcggcgg tcagcctgat gcggctgccg tcgacgatca gcgcgccatc ctcgatcgcg
     2641 acttcgccgg caaagcggcc gtgcaccgag tcgtacttca gcatgtaagc caggtagtcc
     2701 ggctccagca aatcgttgat gccgacgatt tcgacgtcgt cgaaatcacg ggcggcgcga
     2761 aacaccatgc ggccgatacg gccgaatccg tttatgccca cgcgtacagt catagttaaa
     2821 atctccgctt actaaaaaaa ctaaatctta tgcttcgtcg tcgccggtca gtttctttac
     2881 cgcttcgacg accttgccga cggtgaaacc gaagtaggcg aacaactcct tggccggcgc
     2941 cgactcgccg aaactgtcga tgccgatgat aatgccgtgc gtcccgacat acttgtgcca
     3001 ggtcaacgta gacgccgcct cgaccgagat acgcgcggtc acttccggcg gcagcaccga
     3061 gtcgcgatat tcacgggact ggctgtcgaa catcgtcgtg ctcggcatcg ataccacccg
     3121 tatcctgcag ccctcgagct cttccgagtc tgctgccgca accgccagtt cgacctcgga
     3181 accggtggcg atgatgatcg cgtcgggggt accctcgcag tcgcgcagca cgtaaccgcc
     3241 gcgcgcgatg gccgcgatct gctcggcgtc gcgcggctgg aatttgagcg actggcgcga
     3301 aaacaccagg cagctgggac catcgcgatg ctcgatcgcc gccttccagc agaccgcggt
     3361 ttccaccgtg tcgcagggcc gccagacctg catgccggga atcagccgca ggctcggaat
     3421 gtgctcgatg gcctgatgcg tcgggccgtc ttcgccgagc ccgatcgaat cgtgggtata
     3481 gacgaaaatg ctctgcacct tcatcaacgc ggccatgcgc agcgcattgc gcgcataatc
     3541 ggaaaaggtc aggaaggtgg cgccaaaagg cagcagcccg ccgtgcaaca cgatgccgtt
     3601 gatgatcgcc gacataccga attcgcgcac cccgtagttg atatagttgg catccggaga
     3661 ttcggcgcgc atcggcttgt aacccgacca ctcggtgccg ttcgaaccac cgagatccgc
     3721 cgagccgccg aacagttcgg gaagcagcgg cgcaaaggcc tcgatggtcg ccagcgacgc
     3781 ctgccgggta gccttgtcct cgccggcttc gacgacctcg gcgatgaacg cgtctgcctc
     3841 ttcctgccag tgggccggga actcgccgtt gatgcggcgt tcgaattcgg ccgccagttc
     3901 cgggtgcgcc tgccggtact catcgaattt ctgttgccag gcgtcttcgc gggactggcc
     3961 cttgtcgcga tgatcccagc cctggtagat ttcttccggg atcacgaacg catcgtgctc
     4021 ccattccagc gctgcgcgcg tcgcggctat ctcttcctcg cccagcggcg cgccatgaca
     4081 gtcgtggctg ccggccttgt tgggcgaacc gaagccgatg accgtcttgc aacagatcag
     4141 cgacggcctg gcactgattg cgcgcgc
//
LOCUS       DATCZV010000372         3557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_242403, whole genome shotgun sequence.
ACCESSION   DATCZV010000372 DATCZV010000000
VERSION     DATCZV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242403"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VIW27_08320"
     CDS             <1..995
                     /locus_tag="VIW27_08320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY5739717.1"
                     /translation="IIDRGKIIMSDKPVVLVTRKLPDAVEARLTEDFTPLLNPDDRLY
                     DTDELLQLAAGADAILPCHTEKFSAETIERLPARVRAIANYSVGVDHVDLEAAKRRGI
                     IVTNTPDVLSDATAEIAMLLMLGAARRASEGERLVRSQAWKDWSPAFMVGRQITGKRL
                     GILGMGRVGQVVAARARGFEMEIHYHNRNPLPDEHAAGAEYHASVDDLLAAVDVVSIH
                     CPASPQTHGLIDRQRLAAMHADAILVNTSRGGVIDDEALVAALRDGVIAAAGLDVYNG
                     EPDRIHSGYRELDNVFLLPHIGSATVETRYAMGTRAIDNLRAIFGGREPGDRVA"
     gene            1266..2387
                     /locus_tag="VIW27_08325"
     CDS             1266..2387
                     /locus_tag="VIW27_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5739718.1"
                     /translation="MTEEVKKSRRKFLTSGAAAIAGGAAAASFPNISVGQSPVVLKVQ
                     AAWGGGIFLEFAQDYVRRVNEMSGGSLKIDLLGVGAVVKTAEMQTAVHKGVLDGAHLV
                     TAYWYSKSPVASLFGTGPCFGWSANELMGWIAYGGGRDLYYELMHDKLRLDLVGFFSG
                     PMPAQPLGWFKEHITGSDQMKGLKYRTVGLAADVLQEMGMSVVQLPGGEIQPAMKSGL
                     IDAAEFNNPTSDKDFGMQDVSKHYHLGSFHQSQECFEIIFNKTRYEKLAKEHQAILEY
                     ASEAASQDMAWKAMERYSQDLVVLQEKHGVNVYKTPDSVMKDQLKAWDIIVEKFNKED
                     PFFKKVIESQMAWAKRHGSYALRNSPNYAAAYEHYFGKL"
     gene            2495..3103
                     /locus_tag="VIW27_08330"
     CDS             2495..3103
                     /locus_tag="VIW27_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5739719.1"
                     /translation="MTRILHFIDSLSAWTGKAFAWCIVILTFSTCYEVFMRYVLDSPT
                     VWAFDMSVQMYGALFMMAGAYALSQDAHVRGDVVYRLLKRKTQAKIDLALYILFLMPG
                     ALALIWYGYLFAADSWFYKEVSWSSPSRIQIYFFKTLIPISGMLVLLQGIAEAVRCVI
                     CMRTGEWPPRLHDVQETETMLLNEQADLRRMEEEARKEQGHV"
     gene            3096..>3557
                     /locus_tag="VIW27_08335"
     CDS             3096..>3557
                     /locus_tag="VIW27_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5739720.1"
                     /translation="MSDPVVAVVMLGLFIFMVMLGFPIAFTLIAMGLGFGYYAYYDAE
                     RMQTIFDNRIFDLFVQNTFSVMSNDVLVAVPLFLFMGYVVERANIVNRLFFSIQLAAR
                     NLPGSMAVAALFTCAVFATASGIIGAVVTLMGMLAFPAMLKAGYNHSFASGV"
BASE COUNT          736 a         1071 c         1064 g          686 t
ORIGIN      
        1 tcatcatcga tcgcggaaaa atcatcatga gcgataaacc tgttgtactg gtaacccgca
       61 agctccccga tgcagtggaa gccagattga ccgaggattt cacgccgctg ctgaatccgg
      121 acgacaggct ctacgacact gacgaattgc tgcagctggc ggccggcgcc gacgcgatcc
      181 tgccctgcca cacggaaaag ttttccgccg aaaccatcga gcgcctgccc gcgcgtgtcc
      241 gggcgatcgc caattactcg gtgggcgtcg atcacgtcga tctcgaggcg gcgaaacgca
      301 ggggcatcat cgtcaccaac accccggacg tgctgtccga tgcgaccgcc gaaatcgcca
      361 tgctgctgat gctcggcgcg gcgcgccgcg ccagcgaggg tgagcgcctg gtgcgcagcc
      421 aggcctggaa ggactggagc ccggcgttca tggtcggacg tcagatcacc ggcaagcgcc
      481 tcggcattct cggcatggga cgtgtcggcc aggtggtcgc ggcacgcgcg cgcggcttcg
      541 agatggagat ccattatcac aaccgcaatc cgctgcccga cgagcatgcg gcgggggccg
      601 agtaccacgc cagcgtggac gacctgctgg cggcagtcga cgtcgtctcg attcactgcc
      661 cggcctcgcc gcagacacac ggcctgattg atcgccagcg cctggcggcg atgcacgccg
      721 acgcaatcct ggtcaatacc tcgcgcggcg gcgtcatcga cgacgaggcg ctggtcgcgg
      781 cgctgcgcga cggcgtcatc gccgccgcgg ggctcgatgt ctacaacggc gagccggacc
      841 ggattcattc aggataccgc gaactcgaca acgttttcct gctgccgcac atcggcagcg
      901 ccacggtcga aacccgctac gcgatgggga cccgggcgat cgacaacctg cgcgcaattt
      961 ttggcgggcg ggaaccgggc gaccgggtcg cgtaacgccc gcggcgagtg atcagcgagc
     1021 gcctccggat cgactcaacc gagcgccgaa ctcaccgcga accgggataa cgcttgtaca
     1081 tgccaaactt tactggtata caatataccg cgcctaattg cccggattcc cattattcgc
     1141 ctcgatcatg taagatgcag atgggatacg gcaagctcct gtttcagcaa gccgtaaagg
     1201 ccacacgaca aacgtaaaat aacatcggcg tcagctatca catcaataat agagaggaaa
     1261 caatcatgac cgaagaagtt aaaaaatcac gtcgcaaatt cctaacgagc ggcgcggcag
     1321 ccatcgccgg tggagcggca gcggcatcgt tccctaacat ctccgtaggc caaagcccgg
     1381 tagtcctcaa ggttcaggcg gcctggggcg gcggtatctt tctcgaattc gcccaggatt
     1441 acgtgcgccg cgtcaacgag atgtccggcg gctcgctgaa gatcgacctg ctcggcgtcg
     1501 gcgcggtggt caagaccgcc gagatgcaga ccgcggtaca caagggcgtt ctcgacggcg
     1561 cgcacctggt aaccgcctac tggtactcga agagcccggt ggcttcgctg ttcggcaccg
     1621 gcccctgctt cggctggtcg gccaacgagc tgatgggctg gatcgcgtat ggtggcggta
     1681 gggatctcta ctacgagctg atgcacgaca aactgcgcct cgacctggtc ggcttcttct
     1741 ccggcccgat gccggcgcag ccgctgggct ggttcaagga gcacatcacc ggcagcgacc
     1801 agatgaaagg cctcaagtac cgcacggtcg gcctcgccgc ggacgtactg caggaaatgg
     1861 gcatgtcggt ggtacagctg cccggcggtg aaatccagcc ggcgatgaag agcggcctga
     1921 tcgacgcggc cgagttcaac aacccgactt cggacaagga cttcggcatg caggacgtgt
     1981 ccaagcacta ccacctcggc agcttccacc agtcgcagga gtgtttcgaa atcatcttca
     2041 acaagacgcg ttacgaaaag ctcgccaagg agcaccaggc gatcctcgag tacgcttcag
     2101 aagccgcgtc gcaggacatg gcctggaagg cgatggagcg ttactcgcag gatctggtgg
     2161 tactgcagga aaagcacggc gtcaacgtct acaagacccc cgacagtgtc atgaaagacc
     2221 agctgaaggc ctgggacatc atcgtcgaga agttcaacaa ggaagatccg ttcttcaaga
     2281 aagtcatcga atcccagatg gcctgggcga agcggcacgg ttcttacgcg ttgagaaact
     2341 cgccgaacta cgccgctgcc tacgagcact acttcggcaa gctgtaaaga cgggtgtaac
     2401 gcaaaagctg taacctggcg gggcggtatt taacgatacc gcccctttag gtttgcaatc
     2461 gatcgaattt gaataaccaa ccggtggtgt gccaatgaca cgaatactcc acttcatcga
     2521 tagcctgagc gcctggaccg gcaaggcatt tgcctggtgt atcgtcatcc tgactttttc
     2581 aacctgttac gaggtattca tgcgctacgt actcgattcg cccaccgtct gggccttcga
     2641 catgagcgtg caaatgtacg gcgctctatt catgatggcc ggcgcctacg cgttgtctca
     2701 ggatgctcac gtgcgcggtg acgtggtcta ccggctgctg aaaagaaaaa cgcaggcaaa
     2761 gatcgacctg gcgctgtaca tcctgttcct gatgcccggt gcgctggcgc tgatctggta
     2821 cggttacctg ttcgctgccg attcctggtt ttacaaggag gtcagctgga gcagcccgtc
     2881 gcgcattcaa atctattttt tcaaaaccct gatcccgatc tccggcatgc tggtgctgtt
     2941 gcagggcatc gccgaggcgg tgcgctgcgt gatctgcatg cgcaccggag agtggccgcc
     3001 gcggctgcac gacgtgcagg agaccgagac catgctgctt aacgagcagg cggatctgcg
     3061 caggatggaa gaggaagcaa gaaaggagca aggtcatgtc tgatccagta gtcgctgtcg
     3121 tcatgctcgg cctgttcatc ttcatggtga tgcttggctt tccgatcgca tttacgctta
     3181 tcgccatggg tttaggtttc ggttactacg cctattacga tgccgagcgc atgcaaacca
     3241 tattcgataa ccgcatcttc gacctgttcg tgcagaacac cttctcggtc atgtccaacg
     3301 acgtgttggt ggcggtgccg ctatttctct tcatgggcta cgtggtggaa cgagcgaaca
     3361 tcgtcaatcg cctgtttttc agtattcagc tggcggcacg caacctgccc ggttcgatgg
     3421 ccgtcgcggc gctgttcacc tgcgccgtgt tcgcgaccgc gtcgggcatc atcggcgcag
     3481 tggttacgct gatgggcatg ctcgcctttc cggcgatgct caaagcgggc tacaaccaca
     3541 gtttcgcttc cggcgtc
//
LOCUS       DATCZV010000373         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_136318, whole genome shotgun sequence.
ACCESSION   DATCZV010000373 DATCZV010000000
VERSION     DATCZV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136318"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VIW27_08340"
     CDS             complement(<1..779)
                     /locus_tag="VIW27_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5739721.1"
                     /translation="MKVSLDDKYQLDKGRAYMTGIEALVRLPMLQHQRDSERGLNTAC
                     FISGYRGSPIGGLDQALWKAKKFTDAHNIHFLPGVNEDLAMTAVWGSQQVGLFPGAQY
                     DGVFGLWYGKGPGLDRSMDVLKHANAFGTSRYGGVLAVVGDDHACKSSTLPHQSDHMF
                     VGATSPVLNPAGVQEILDLGVFGWELSRYSGCWVGLKAITENMDAAISAEIDPGRVEV
                     VIPEDWELPPDGVHSRWPDTPMAQEERLQRHKIYAARAFARA"
     gene            918..1400
                     /locus_tag="VIW27_08345"
     CDS             918..1400
                     /locus_tag="VIW27_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017709049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5739722.1"
                     /translation="MFMKLSPVDIRILSLLQENASLTAAEIAEQVNLSPSPCWRRINR
                     LEQDGVIEKKVALLDAEKLGLGLVVFSRISLAKNDEASLHEFEERVRAHPEVVECYTV
                     TGSADYFLKIITRDIKHYDRFLRRHLIQMPLVRDVNSNVAVTQIKYTTSLPLETQLSA
                     "
     gene            1525..1968
                     /locus_tag="VIW27_08350"
     CDS             1525..1968
                     /locus_tag="VIW27_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5739723.1"
                     /translation="MIKKILVATDASAASARAVSYAARLSVLHEADLLILNVIRDMQL
                     PAEMREMPEFENFFNTREDLMRQAAERILDAAKQMARQDGARNVQTAIGSGDPATSVA
                     GFARRRRIDLIVVGTRGLSKMQASAMGSVSRKLLDLTDVNCLVVR"
     gene            complement(1978..>2876)
                     /gene="gnd"
                     /locus_tag="VIW27_08355"
     CDS             complement(1978..>2876)
                     /gene="gnd"
                     /locus_tag="VIW27_08355"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEY5739724.1"
                     /translation="MVHNGIEYGDMQLICEAYQLMKELLGMSVDEMHAVFADWNQGEL
                     DSYLIEITRDILGYRENGEPLVEKILDTAGQKGTGKWTGIVALELGTPLTLIGEAVFA
                     RFLSAQKEEREQAARLFDGPERRYAGDRGALVEDIRQALLASKIVSYAQGYLLMREAA
                     QQYDWNLDYGGIALLWRGGCIIRSVLLQRIKEAFDAQPELPNLMLSAYFRDKIETAQA
                     GWRRIAAAAVSHGIASPALTSALCYLDGYRSARLPANLLQAQRDYFGAHSYERVDRPR
                     GEFFHTNWTGRGGDTASSTYNV"
BASE COUNT          550 a          889 c          869 g          568 t
ORIGIN      
        1 gcgcgcgcaa aggcgcgcgc ggcatagatc ttgtggcgct gcagccgctc ttcctgggcc
       61 attggcgtat ccggccagcg cgagtgcacg ccgtccggcg gcagctccca gtcctcgggt
      121 atcacgacct cgactcgtcc gggatcgatc tccgccgaaa tcgccgcatc catgttctcg
      181 gtgatcgcct tcaggcccac ccagcatccg ctgtaacgtg acagttccca gccaaagacg
      241 ccgaggtcga gaatttcctg cacgccggcg ggattgagca ccgggctggt ggcgccgacg
      301 aacatgtggt cgctctggtg gggcaacgtc gacgatttgc aggcgtgatc gtcgccgacc
      361 accgcgagca cgccgccgta gcgcgaggtg ccgaaggcgt tggcgtgctt gaggacgtcc
      421 atgctgcggt cgagacccgg gcccttgcca taccacaggc cgaatacgcc gtcgtactgc
      481 gcacccggga acaggccgac ctgctggctg ccccagacag cggtcatcgc caggtcctcg
      541 ttgacgccgg gcaggaaatg gatgttgtgt gcgtcggtaa atttcttcgc tttccacagc
      601 gcctggtcga ggccgccgat cggcgagccg cgatagccgg agatgaaaca ggcggtattg
      661 agaccgcgct cggaatcgcg ctgatgctgc agcatcggca gccgcaccag ggcctcgata
      721 ccggtcatgt aggcgcggcc cttgtccagc tggtacttgt cgtcgagaga gactttcatg
      781 gcaaacccac cgtgcataat gggtaatttg ctgattctag tcctctgtcg caggaaatgt
      841 attgcaatat tgcaatggtt taacgagaat tagcaaaaat attttaatat ttaattaaaa
      901 tttagaaaat aattccaata tttatgaaac tttcgccggt cgatatcagg atactgtcgc
      961 tgctgcagga aaacgcgtcg ctgaccgccg ccgaaatcgc cgagcaggtc aacctgtcgc
     1021 cgtcgccgtg ctggaggcgt atcaaccggc tcgagcagga cggcgtgatc gagaagaaag
     1081 tcgccttgct cgatgccgag aagctcggtc tcgggctggt ggtgttttcg cgcatcagcc
     1141 tggcgaagaa cgacgaggct tcgctgcacg aattcgagga gcgggtgcgc gcccaccccg
     1201 aagtcgtcga atgctacacg gtcaccgggt cggccgacta ctttctcaag atcatcaccc
     1261 gcgacatcaa gcattacgac cgctttctgc ggcgccacct gatccagatg ccgctggtgc
     1321 gggacgtcaa ctccaacgtc gcggttacgc agatcaagta caccacctcc ctgccgctcg
     1381 aaacccagct ttcggcctga tcccgtgcca ggcccggtca tcgcaattcg cgtgattgtg
     1441 acgacttgat aacagggctt gcactatcgg ttgcgtgaag gcttaatacg cgttcctcga
     1501 ggctgaagtg gtgcctttca ccggatgatc aaaaaaatac tggttgctac cgacgcatcc
     1561 gcagcgtcgg ctcgagcggt cagttatgcc gcgcgtctgt cggttctgca cgaggccgac
     1621 ctgttgattc ttaacgtgat tcgcgacatg cagttgcccg ccgaaatgcg cgaaatgccg
     1681 gaattcgaaa atttcttcaa cacgcgcgaa gacctgatgc gccaggccgc ggagagaatt
     1741 ctcgacgcgg ccaaacaaat ggcgcgccag gatggtgcac gcaatgtgca aaccgcgatc
     1801 ggcagcggcg atccggcgac cagcgttgcc ggttttgcgc gccggcgcag aatcgacctg
     1861 atcgtcgtcg gcactcgcgg cctgtcgaag atgcaggctt cggcgatggg cagcgtgtca
     1921 cgcaagctgc tcgatctgac cgacgtcaac tgcctcgtcg tgcgctgata cccgggtcta
     1981 gacgttatag gtcgacgacg cggtatcgcc gccgcgaccg gtccagttgg tgtgaaagaa
     2041 ttcgccgcgc ggccgatcga cgcgttcgta gctgtgtgcg ccgaaatagt cgcgctgcgc
     2101 ctgcagcagg ttcgccggca ggcgtgccga gcgataaccg tcgaggtagc aaagcgccga
     2161 ggtcagcgct ggtgatgcga taccgtggct gacggccgcc gcggcgatcc gccgccagcc
     2221 ggcctgcgcg gtttcgatct tgtcgcgaaa ataggctgac agcatcaggt tcggcagctc
     2281 cggctgcgcg tcgaaagcct ccttgatgcg ttgcagcagc accgagcgaa tgatgcagcc
     2341 gccgcgccat aacagtgcga ttccgccgta gtccaggttc cagtcgtact gctgcgccgc
     2401 ctcgcgcatc aacaggtagc cctgcgcgta ggaaacgatc ttggaggcca gcagggcctg
     2461 gcgtatatcc tcgaccagcg cgccgcggtc acccgcgtaa cggcgctcgg ggccgtcgaa
     2521 caagcgggct gcctgctcgc gttcctcttt ttgcgccgac aggaagcgcg caaacaccgc
     2581 ctcgccgatc agcgtcagcg gcgtgccgag ctcgagcgcc acgatgccgg tccacttgcc
     2641 ggtacccttc tggccggcgg tatcgagtat cttttcgacc agcggctcgc cgttttcgcg
     2701 gtaaccgaga atgtcgcgcg tgatctcgat caggtagctg tccagttcgc cctggttcca
     2761 gtcggcaaac acggcgtgca tttcgtccac gctcatcccc agcaattctt tcatcaactg
     2821 gtaagcctcg cagatcagct gcatgtcgcc gtactcgata ccgttgtgca ccatct
//
LOCUS       DATCZV010000374         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_718875, whole genome shotgun sequence.
ACCESSION   DATCZV010000374 DATCZV010000000
VERSION     DATCZV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718875"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VIW27_08360"
     CDS             <1..433
                     /locus_tag="VIW27_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5739725.1"
                     /translation="GLTPEVLNIVGTPSEAKMRQVNEGSANLLKWAKKHGVRIAWGTD
                     LFGPPAKQAQQPQEFIARAAYFTPFEILRQATSDNAELFELSGLRHPYQQGALGVVQK
                     GAYADLLLVDGNPLQNIELMTDPGKNFRVIMKDGKVFKNTL"
     gene            complement(691..1506)
                     /locus_tag="VIW27_08365"
     CDS             complement(691..1506)
                     /locus_tag="VIW27_08365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739726.1"
                     /translation="MQWMKRPASDTQITHGDRLWLYIVAAIIMLLLVIPTFIVIPMSF
                     SDSQYLEFPPENWSTRWYEVYFESPKWMRATVTSFQVGVLTMLVATPLGMLAAYALFV
                     SGHRAARAIFAFLITPMIVPVILIAIGTFYAFGRTGMNNTITGIVLAHTVLASPLVMI
                     VITAALRNYDLNQERVARSLGATRTRAFFVITLPQIKFSVVIAALLSFLTSFDEVIIA
                     IFVSGGVNATLTKHMFAALRDFIDPTIAAISTIMILVSTTLLLVTLFFEHKGK"
     gene            complement(1508..2332)
                     /locus_tag="VIW27_08370"
     CDS             complement(1508..2332)
                     /locus_tag="VIW27_08370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739727.1"
                     /translation="MLLSLAMPAILAVVAIIVIPVGWLFYLSFVDGDGQFSLQNYQRM
                     IEYKSYARVFLTTFQVSFLTTLICILIGYPLAYFLAMLPARWAGLFMLTVLLPFWTSL
                     LVRTYAWLVLLQKNGLLNDFAISAGIWETPLKLVHNMTGTLIGMAHIMLPFLILPLYG
                     SMRKIDRDLMYASANLGASPVQSFWRVYFPLSLSGMVAGSLIVFVLCLGFYVTPAVLG
                     GGRVVMVATQITAILENQYDWGAASALGVVLLVATLLVLFAATRLLKLDAALFGRH"
     gene            complement(2413..>2998)
                     /locus_tag="VIW27_08375"
     CDS             complement(2413..>2998)
                     /locus_tag="VIW27_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739728.1"
                     /translation="KKLREQMQIELKHLHEKLGVTTVYVTHDQREALTMSDRIAVIND
                     GKLVQIDTPHNIYNHPANAFVADFIGESTILPLTRDNSGRLYFEDHLIESDLQTAVGS
                     EWSLVIRPERLTVLKSGEAVDSEQTMVFPGEVRDFVFQGETAFALLSVGGRHLLSFRF
                     ATDSQSQYNLQPGEQISLGLKRHDLIVIPRGDGQ"
BASE COUNT          673 a          865 c          903 g          557 t
ORIGIN      
        1 cggcctgacg cccgaggtgt tgaacatcgt tggaaccccg agcgaagcga agatgcgtca
       61 ggtgaatgaa gggtcggcaa acctgttgaa atgggccaaa aagcacggcg tcaggatcgc
      121 ctggggcacc gatctgttcg gtccgccggc caagcaggcg caacagccgc aggaatttat
      181 cgcgcgcgcg gcgtatttca cacccttcga aatactcagg caggcaactt cagacaatgc
      241 cgaattattc gaactgagcg gcttgcgcca cccctatcag cagggtgcgc tcggcgtggt
      301 gcaaaagggt gcctatgcgg acctgctgct ggtcgacggt aatcctttgc agaacatcga
      361 gctgatgacc gatcccggca agaattttcg ggtcatcatg aaagacggca aggttttcaa
      421 gaataccttg tgagtgtctc actagggtcg cctgccgccc agcccaaaat tctgagcgtc
      481 agctttttta aattgagggt atctgtaatt tcgcgatgca ctcgaaagct gccctgacct
      541 cacgcgaggg cgccaagcaa aaagttcgat atgcgccaac gagggggagc caaattccga
      601 aaagccggtt tgaatgcgtt cagccggctt tttcgtttcg attttcgagt gctgcagttt
      661 cgggacccgc cagatgggac cactgcctgc ctacttcccc ttgtgctcga aaaacagggt
      721 aaccagcaac agggtcgtgg aaaccaggat catgatcgtc gatatcgccg cgatcgtcgg
      781 atcgatgaaa tcgcgcaacg cggcgaacat gtgtttcgtc agcgtggcat tcacgccgcc
      841 ggagacgaat atcgcgatga tcacctcgtc gaacgaggtc aggaaagaca gcagcgcggc
      901 gattaccacc gaaaacttga tctgcggcag cgtaatcacg aagaacgccc tggtccgggt
      961 cgcgcccaga ctcctcgcga cccgctcctg gttgaggtcg tagttgcgca gcgcggcggt
     1021 gatcacgatc atcaccagtg gcgaagccag taccgtgtgc gcgaggacga tgccggtaat
     1081 ggtgttgttc atgccggtgc gaccgaaagc gtaaaaggtg ccgatcgcga tcaggatcac
     1141 cggcacgatc atcggtgtga tcaggaaagc gaaaatcgcc cgcgcggccc ggtgcccgga
     1201 aacgaacagc gcataggccg ccagcatgcc gagcggcgtg gccaccagca tcgtcagcac
     1261 accgacctgg aaggaggtca ccgtggctcg catccatttg ggtgattcga aatagacttc
     1321 gtaccaacgc gtggaccagt tttcgggcgg aaactccagg tattgagaat cggagaacga
     1381 catcgggata acgatgaacg tcggaatcac cagcagtagc atgatgatcg ccgcgacgat
     1441 gtacagccac aaccggtcac cgtgggtgat ctgggtatcc gaggccgggc gtttcatcca
     1501 ctgcatgtca atgcctgccg aacagggccg cgtcgagttt taacaaccgc gtggccgcga
     1561 acaataccag cagcgtcgcg accagcagca ccacgccgag cgcgctggcc gcaccccagt
     1621 cgtactggtt ttcgagtatc gcggtgattt gcgtggccac catgaccacg cggccgccgc
     1681 cgaggaccgc cggggtcacg taaaaaccga ggcagagcac gaacacgatc agcgaaccgg
     1741 ccaccatgcc cgacagcgac aatggaaagt aaacccgcca gaaggactgc accggggacg
     1801 ctccgaggtt ggccgacgcg tacatcaggt cgcgatcgat cttgcgcatc gagccgtaca
     1861 gcggcaggat caaaaacggt agcatgatgt gcgccatacc gatcagcgtg ccggtcatgt
     1921 tgtgcaccag cttgagcggc gtctcccaga tgcccgcgga aatcgcgaaa tcgttcagca
     1981 ggccattttt ctgcagcagc acgagccagg cgtaagtgcg caccagcagc gaggtccaga
     2041 acggcagcaa taccgtgagc atgaacaacc cggcccatct ggcgggcagc atcgccagga
     2101 aataggcgag cgggtaaccg atcaggatac agatcagcgt ggtcaggaag ctgacctgga
     2161 aggtcgtcag gaatacgcgg gcataggatt tgtactcaat cattcgctgg taattctgca
     2221 gcgaaaattg cccatcgcca tcgacgaaag agaggtagaa cagccagccg accgggatca
     2281 cgataatcgc cactacggcc aggatggccg gcatcgcgag gctcagcagc atgcgccgct
     2341 cgcgaacgcc ctgcaccctg agcgtattct cgttcaggcc ctccccgtgg cggccgtctt
     2401 gctgcgcggc gttcactgcc cgtcgccccg cgggatcacg atcaggtcgt ggcgtttcag
     2461 gccgagggag atttgttcgc ccggctgaag attatattgc gactgcgaat cggtcgcgaa
     2521 gcggaacgac agcaggtgcc ggccgccgac cgacagcaag gcgaacgcgg tctcgccctg
     2581 gaaaacgaag tcccggacct cgccgggaaa caccatcgtc tgctcgctat cgaccgcttc
     2641 gccgcttttc agtaccgtca gcctctcggg gcgaatgacc agcgaccatt cgctgcccac
     2701 agcggtttgc agatcgcttt cgatcaggtg gtcttcgaaa tacaaccggc ccgaattgtc
     2761 gcgggtcaac ggcaggatgg tcgattcacc gatgaaatcg gcgacgaacg cgttggccgg
     2821 gtggttgtag atgttatgcg gcgtatcgat ctgcaccagt ttgccgtcgt tgatcaccgc
     2881 gatgcggtcg gacatggtca gggcttcgcg ctgatcgtga gtcacgtaga cggtggttac
     2941 gcccagcttt tcgtgcaggt gtttaagttc gatttgcatt tgttcgcgca acttttta
//
LOCUS       DATCZV010000375        16123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_136523, whole genome shotgun sequence.
ACCESSION   DATCZV010000375 DATCZV010000000
VERSION     DATCZV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16123
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136523"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1494
                     /locus_tag="VIW27_08380"
     CDS             82..1494
                     /locus_tag="VIW27_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008842610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5739729.1"
                     /translation="MDKNVSSRTTLEKALRINLDEKKYGTIVEIGAGQEVARQFFKTG
                     GAAGTIAKTMSAYDMRFSDAIYGVQEDRRYVSRNRVLAMLDKEFQLVIDRVGKYRSAS
                     SRYFAYAATVAAKSYKRDNECHAWCGIRVQMYPGAEPSNIVAHVRMRDSNAEQQQQAL
                     GVFGVNLIYASYYYFEDPREIIDSLTDNLAADRIEIDSIDFEGPYFEEVDNRAINFHL
                     IRSWKTRAVMFNSDGSVEVPAEMLYKKNVLAIRGTFKPVTRLNVDMIEQGLKFFTSME
                     GVTLDNSVALAEISLNDMQGNDLGVSGEDLIARVGLLNKLGYSVMVSDHTRYFSMRAY
                     FRQFTELQIGIVVGIVNIRQIFDQSMYRGVEGGILEGFGKLFPDNTRLLVYPEIDSAG
                     ELIDYCNVKVPPNLQYLYQHLLENHFIFGIDNSDPALFNIFSREVLKQLPNGRGSWEK
                     ALPDGVAEEIIAKKLFGYRD"
     gene            complement(1511..2725)
                     /locus_tag="VIW27_08385"
     CDS             complement(1511..2725)
                     /locus_tag="VIW27_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5739730.1"
                     /translation="MAGPLAGVRVLDLSRILAGPWASQMLADLGAEVIKIERPASGDD
                     TRGWGPPFMPGEDGSPGTESAYFHAANRGKQSVCIDMSLPAGQALIRELACRSDVLIE
                     NFKVGGLQKYALDYASLEALNPALVYCSITGFGQQGPYAARAGYDFMIQAMGGMMSVT
                     GEPDGQPMKIGVALADVLTGLYAANAIQGALLHQRATGEGQYIDMALLDVQVAALANQ
                     AMNYLATGVSPRRLGNAHPNIVPYQAFATSDGHIILAVGNDAQFARFCALAGRAELAA
                     DARFRTNSARVEHRDRLVPQVVEIMRARSSAQWLEALNGAGIPCGPINDIEQVFDDPQ
                     VRFRGLQMQLDHPAAGRVASVANPIRLSRTPVEYLAAPPLLGQHTDDVLARVLQLDAA
                     RIEALRGDGVIG"
     gene            complement(3003..4187)
                     /locus_tag="VIW27_08390"
     CDS             complement(3003..4187)
                     /locus_tag="VIW27_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007637935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5739731.1"
                     /translation="MTQAKRVSFDWQDPMFLEQQLDEDERMIRDATREYCQQQLLPRV
                     LQAHRDEVFDREIMNEFGELGLLGATLPEEYGCAGANYVSYGLVAREVERVDSGYRSA
                     MSVQSSLVMYPIYAYGSDEQRHKYLPGLARGELVGCFGLTEPDAGSDPASMKTTATAV
                     DGGYRLKGAKMWITNSPIADVFMVWAKLDGVIRGFILERDMDGLSTPKIEGKFSLRAS
                     VTGEIAMQDVFVPEANLLPNVEGLKGPFGCLTRARFGIAWGSMGAAEFCWHAARQYTL
                     DRSQFGRPLAATQLVQKKLADMQTEITLGLQGCLRMGRLMDDDNCPVELVSLMKRNNC
                     GKALDIARLARDMHGGNGVADEYHVIRHLMNLEAVNTYEGTHDIHALILGRAQTGIQA
                     FG"
     gene            complement(4285..5577)
                     /locus_tag="VIW27_08395"
     CDS             complement(4285..5577)
                     /locus_tag="VIW27_08395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5739732.1"
                     /translation="MPDHQPQDPGENSLWAATAIAAPACERLREAVRADVLVVGGGYT
                     GLSSALHLAAAGVDVVLLEGRRLGYGGSGRNAGLVNAGVWRNPRHVVEQLGTARAERF
                     NLALRDSPALVFGLVERHAMECDARRVGTIQIAHSAAALKDLEERCRQIQGLGAAVEM
                     IDASTAARLSGSPLYRHGGIVDPGAGTIHPLAYVRSLAHAALAAGARIFEESAILGLE
                     QTAQGWRATTAAGEVSAERVILATNAYADANSSRVREATVPVYIFQCASEPLNPELAA
                     ELIPERHGLWDTQPLMTSSRIDGAGRLIMSSAGQLAGYQRPVREDWMRRNRERLFPQT
                     RGLRWEYRWSGRVGVTDSRILRVQLLAPGVYAPAGYNGRGIGPGTVIGKHLAELIAGG
                     SEQDFPFPLQPLHRESWRGLRAAGYSYGTLALQFVDRR"
     gene            5872..6903
                     /locus_tag="VIW27_08400"
     CDS             5872..6903
                     /locus_tag="VIW27_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY5739733.1"
                     /translation="MLTVYSEKHALRDAATELYGGRLVPPFECPVRAEHVLARVREVG
                     LGEIVTPREFGIDAITRIHDADFIRFLETCWQDWTAAGYEGEAIPNCWPARGMQQQRV
                     PRHIEGKLGHYAMAAETSISGGTWEAARASADVALTAQAALADGAREAFALCRPPGHH
                     AAASLFGGYCFINNAAVAAQAFLDQGASRVALLDVDFHHGNGSQAIFYRREDVMFLSL
                     HGDPDDAFPHFLGYADETGEGAGAGFNHNYPMGPGTGFDTWGAALDDACRKIAGYAPD
                     ALVVSLGVDTFERDPISFFRLGSEDFTRYGATIGRLGLPTLFLMEGGYAVEEIGINAV
                     NVLQGFEGR"
     gene            6914..7876
                     /locus_tag="VIW27_08405"
     CDS             6914..7876
                     /locus_tag="VIW27_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739734.1"
                     /translation="MKRLAGLGPNTRGVLCLVIAIAWLTVSDAIIKWLSPELPLHQIM
                     LLRSLVALPLVLLIAHLEGGWRTLKTQRPLAHLLRGSLLVLANMCFFTALAAMPLAET
                     VTLFYTAPLFICLLSWPLLGERVGAARWIAIAVGMAGVVIMMQPGAELFRPVSVLPVL
                     AALSYAAMTMMTRKLGLRETAGALTFYIQIAFIVISAIVGLLIGDGGFDNFDNPSLQF
                     LLREWRWPNAIESQLLIACGAIVTIGGYLISQAYRIGEAPAVAPFEYASLPFALAVGF
                     ALWGDWPNARAFLGTGLIVGSGLVVVYLENRAYRKSLRPAPIDL"
     gene            7970..8302
                     /locus_tag="VIW27_08410"
     CDS             7970..8302
                     /locus_tag="VIW27_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY5739735.1"
                     /translation="MTEADRLSQRLLDMKSELERRLRSIDRDIRHEGMSADWSEQASE
                     RENDEVLESLGSNSEKELRMIEQALRRIDDGSYFRCDNCGEEIPEARLELLPFTPYCV
                     NCAEQLQP"
     gene            8313..8552
                     /locus_tag="VIW27_08415"
     CDS             8313..8552
                     /locus_tag="VIW27_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY5739736.1"
                     /translation="MLTDNHDLLHEFPEYRDTIHQLKLGNAHFSRLFDEYHEIDHEVH
                     RIETGVENTSDEYLEERKKMRLYLKDELYRMIRET"
     gene            complement(8549..8905)
                     /locus_tag="VIW27_08420"
     CDS             complement(8549..8905)
                     /locus_tag="VIW27_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5739737.1"
                     /translation="MKPIADSERLIANIDEAEFEPFINDQGKQDGWVLQLNKSKPAGT
                     GFHIYRMDPGYTTLPHEHLGAEEFYVISGDAVDNDGTVYRPGDLVLLKSGTQHTTYSE
                     HGCTVVCYLPDSRSVD"
     gene            complement(8907..9332)
                     /locus_tag="VIW27_08425"
     CDS             complement(8907..9332)
                     /locus_tag="VIW27_08425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5739738.1"
                     /translation="MPTHKRIRMFNTRDTYPNQALDNDLCQAVVAGNTVYLRGQVGTD
                     FDGKLVGVGDPRAQAEQAMKNVHQLLHEAGSDLSHIVKTTTYITDPRYREPVYQEVGK
                     WLKGVYPISTGLVVAGLGQPEWLMEIDVIAVIPETVTED"
     gene            9633..10949
                     /locus_tag="VIW27_08430"
     CDS             9633..10949
                     /locus_tag="VIW27_08430"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5739739.1"
                     /translation="MPDNSSAAPIETIDTLVVGGGQAGIAISEHLGRCAIPHLVVERD
                     RIAERWRSGRWDSLVANGPAWHDRFPGMEFTGLDADAFAPKEMVADYFVAYAEMIAAP
                     IRCGVEVTRAQRQAGGAGFRVETTAGVIETDNLVAATGPFQQPVIPPVVPADPSIRQL
                     HSSDYRNPGQLPAGAVLVVGAGSSGVQIAEELMHSGRQVYLSVGPHDRPPRAYRGRDF
                     VWWLGILGKWDAVALDPGTEHVTIAVSGAHGGHTVDFKRLAAQGMTLVGMTQSCRDGV
                     LRFAPDLARNIARGDANYLSVLDEADDYAERYGLDLPTDPAARELEPDPPCVTDPLLE
                     LNLAEAGVTAIVWATGFTTDFGWLQVEAFDEHGKPRHQRGIGAAPGLYFLGLPWLSHR
                     GSSFIWGVWHDAAFIADHIATRRRYRGYRPAPAAVANAAQDAHGDG"
     gene            11064..11735
                     /locus_tag="VIW27_08435"
     CDS             11064..11735
                     /locus_tag="VIW27_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY5739740.1"
                     /translation="MTLSIAAWDETSGQLGAAIASSSISVAARCLHWRPGIGVALSQN
                     ITDPRLGPRLLALLADGVSVADALAAVGRSTPHIAYRQLVVVDSTGNTGLFTGDQALG
                     RFAAAEGRHCAAAGNLLADAAVPGAMTAAFENAAGSLGTRLLAALCAGADAGGEAGPL
                     HSAGLLVGGEPGWPLAELRIDWSDDPLDALRTGWAVYEPQIDDYVTRAYDPTRAPSYG
                     VPGDP"
     gene            11736..12899
                     /gene="argE"
                     /locus_tag="VIW27_08440"
     CDS             11736..12899
                     /gene="argE"
                     /locus_tag="VIW27_08440"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY5739741.1"
                     /translation="MNSEQILARLVAFDTTSTRSNLELIDFVRNLLDEHGIASQLLHN
                     DEQTCANLYATIGPDDRGGIMLSGHTDVVPTAGQDWHSDPYRMKTGDDRLYGRGCCDM
                     KGFIACTLAALPELTARPLQTPVHLAFSYDEEIGCVGAKRLVAAMSGFEVRPRLGLIG
                     EPTSMQLVLGHKGKVAYRVTVSGLSCHSAYIDQGVNAVEYAAELIAFIRAMNERARAR
                     LSDPAYSVPHSTFHVGNIDGGTALNIVPRQCHFEFEIRNLPQQDLDELAHEIRHYAED
                     TLLPEMRSRYPQSTIEFEEISSYPGLHTDPAAGVITRVRELNPLAEIGDNVSFGTEAG
                     LFDSLGIECLVCGPGSIEQAHKPDEFVGRAQLQHCDRMLENLAQHCRETAESA"
     gene            12922..13395
                     /locus_tag="VIW27_08445"
     CDS             12922..13395
                     /locus_tag="VIW27_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HEY5739742.1"
                     /translation="MSADKDKLCIGWREWVALPDLNLPAIKAKVDTGARTSALHAFLI
                     EPYTRDGTDMLRFMIHPIQKNQELAVECHCPVFDYRQVTDSGGHREMRYVIQSQVVIG
                     GKQWPIELTLTNRDTMRFRMLLGRRAMEDRFVVDPGASYVNGRLRPRKLYVIQQG"
     gene            13400..14302
                     /gene="rimK"
                     /locus_tag="VIW27_08450"
     CDS             13400..14302
                     /gene="rimK"
                     /locus_tag="VIW27_08450"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEY5739743.1"
                     /translation="MKIALLSRNRRLYSSRRIIEAAEKRGHEIVVIDVLRAYMNIAAH
                     KPSIHYKGEALEGFDAVIPRIGASVTNYGTAVLRQFEMMGVVSLNESVAITRSRDKLR
                     SLQLLSRRGIGMPLTGYANKPDNIKDVIKMVGGAPVVVKLLKGTQGIGVVLAETQKAA
                     ESVIEGFMGVKADILIQEFIKEAGGSDIRCFVIDGKVAAAMKRQGAKGEFRSNLHRGG
                     TSTLIRITPEERSTAVRAAKIMGLNVAGVDLLRSNHGPVVMEVNSSPGLEGIESSTGK
                     DIAGMIITNLEKLKPGKTRTRGKG"
     gene            14403..15461
                     /locus_tag="VIW27_08455"
     CDS             14403..15461
                     /locus_tag="VIW27_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY5739744.1"
                     /translation="MAAKSQGSIDIAGVTVKPGNRQSIDIPLPSFYTHSSVNMPVHVV
                     RGRKPGPVLLVCATIHGDEITGVEIIRRLLTHKFINRIRGTLIAIPVVNVYGFVSKSR
                     YLPDRRDLNRSFPGSESGSMASRLAHVLMTEILPHCTHIVDLHSGAVNRENLPQIRAK
                     LKNEPELEALAHAFGVPVILDMNLVEGSLREAAYERDIPILVYEAGEALRFNEVAIRA
                     GVKGVLNVMAALDMRLRKRKASKTPAMIANASRWVRAPQSGILRSLVTIGARVAVGDV
                     LAYINDPLGENNNEIVSELEGIVIGRSNLPLVFAGEAVFNIASYASVEPVSDRIEAYH
                     EELAPNGDVPNNEEPPLV"
     gene            complement(15649..>16123)
                     /locus_tag="VIW27_08460"
     CDS             complement(15649..>16123)
                     /locus_tag="VIW27_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5739745.1"
                     /translation="AARWAVIAIGLIGLFNIIGSLASGIIGQRYPKPMLLVWIYLLRS
                     VLVTLFLLIPASPATVVAFSIVMGLLWLSTVAPTNALVAVIFGTRYLGMLGGIVFLSH
                     QVGSFLGVWLGGVLYDATGSYDVIWWLGVALGVFAAVVHWPIREEPVERPAASAA"
BASE COUNT         2945 a         5398 c         5010 g         2770 t
ORIGIN      
        1 cccgtggcgg gtcgatataa tgcgctggac tcgtggcggg caatcgagtg caatcaatca
       61 ccggcaaacc cgagcaatat aatggataag aacgtcagca gccgcaccac actcgaaaaa
      121 gcgctgcgca tcaatctcga cgaaaagaag tacggcacca tcgtcgagat cggcgccggc
      181 caggaagtgg cgcgccagtt tttcaagacc ggcggtgccg ccggcaccat cgccaagacc
      241 atgtcggctt acgacatgcg gttctcggac gccatctacg gggtgcagga agaccgccgc
      301 tacgtcagcc gcaaccgcgt gctggccatg ctcgacaagg aattccagct ggtcatagat
      361 cgcgtcggca aatatcgctc cgcatcgtcg cgttatttcg cctacgccgc gacggtggcg
      421 gccaaaagtt acaagcgtga caacgaatgc catgcctggt gcggtattcg cgtgcagatg
      481 taccccggcg ccgagccgtc gaatatcgtc gctcacgtgc gcatgcgcga cagcaacgcg
      541 gaacaacagc aacaggcgct cggcgtgttc ggcgtcaacc tgatttacgc gtcttactat
      601 tacttcgaag atccgcgcga aatcatcgac tcgctcaccg acaacctggc ggccgaccgc
      661 atcgagatcg attcgatcga tttcgaaggc ccctacttcg aggaagtgga caatcgcgcc
      721 atcaactttc acctgatccg cagttggaag acccgcgcgg taatgttcaa ttccgacggt
      781 tcggtggagg tacccgccga gatgctctac aaaaagaacg tgctcgccat tcgcggcacc
      841 ttcaaaccag ttacccggct gaacgtcgac atgatcgagc agggcctgaa gtttttcacc
      901 agcatggaag gcgtgacgct cgacaacagc gtggcgctgg cggagatttc tctcaacgac
      961 atgcagggca acgatctcgg ggtttccggc gaagacctta ttgcccgcgt cggcctgctc
     1021 aacaagctcg gttacagcgt catggtctcg gaccacacgc gttatttctc gatgcgcgcc
     1081 tactttcggc agttcaccga gctgcagatc ggcatcgtcg tcggcatcgt caacattcgc
     1141 cagatcttcg accagagcat gtaccgcggc gtcgagggcg gcatcctcga gggtttcggc
     1201 aagctgtttc ccgacaatac ccgcctgctg gtttaccccg aaatcgacag cgccggcgaa
     1261 ctcatcgatt actgcaacgt caaggtgccg ccgaacctgc agtacctcta ccagcacctg
     1321 ctggaaaacc atttcatctt cggcatcgac aacagcgatc ccgcgctgtt caatatattc
     1381 tcccgcgaag tgctcaagca attgcctaac ggcaggggca gctgggaaaa agcgctaccc
     1441 gacggcgttg ccgaggaaat catcgccaag aaactgttcg gctaccgcga ctgacgcggc
     1501 cggccgggcg tcaaccgatc accccatcgc cgcgcaacgc ctcgatacgc gccgcgtcga
     1561 gttgcagcac ccgcgccagc acgtcgtcgg tatgctgccc gagcagcggc ggcgccgcaa
     1621 ggtactccac cggcgtgcgt gacaggcgta tcgggttggc gacgctcgcc accctgcctg
     1681 cagccgggtg atccagctgc atctgcagcc cgcggaaacg tacttgtgga tcgtcgaaca
     1741 cctgctcgat atcgttgatc ggcccgcacg gaataccggc gccattgagc gcttccagcc
     1801 attgcgcact ggacctggcg cgcatgattt cgaccacctg cggcaccagc cggtcgcggt
     1861 gctcgacgcg cgcgctgttg gtccgaaagc gcgcgtcggc agccagctcg gcacgcccgg
     1921 cgagcgcgca gaaacgcgcg aactgggcat cgttaccgac cgccagaatg atgtgaccgt
     1981 cgctggtcgc gaacgcctgg tagggcacga tattcgggtg cgcgttgccg agccggcgcg
     2041 ggctgacgcc ggtggccagg taattcatcg cctggttggc gagcgccgcg acctgcacgt
     2101 cgagcagtgc catgtcgatg tactgcccct cgccggtcgc gcgctggtga agcagcgcgc
     2161 cctgaatcgc gttggccgcg tacagcccgg tcagcacgtc ggccagcgcg acaccgatct
     2221 tcatcggctg accatcgggc tcgccggtga cgctcatcat gccgcccatc gcctggatca
     2281 tgaaatcgta accggcacgc gccgcgtagg gcccctgctg gccgaagccg gtaatcgagc
     2341 aatacaccag cgccggattg agcgcctcga ggctggcgta atcgagcgcg tatttttgca
     2401 ggccgccgac cttgaagttc tcgatcagca cgtcgctgcg gcaggcgagt tcgcggatca
     2461 gcgcctgccc ggcgggcagc gacatatcga tgcaaaccga ttgcttgccg cgattggcgg
     2521 cgtggaagta ggccgactcg gttcccggac tgccgtcctc gccgggcatg aacggcggtc
     2581 cccagccgcg cgtatcgtca ccgctcgccg ggcgctcgat cttgatcacc tcggcgccga
     2641 ggtcggcgag catctggctg gcccaggggc cggcgagaat ccggctcagg tccagtacgc
     2701 gaaccccggc gagcggcccg gccatcctat cccctgtatt gcatcggcga cccgcaggag
     2761 cgcaatcggt gcgtagcggc gggacaggag cccagggacg ccgcgccgca acagcgacgg
     2821 cgtacggggt tagcagaccg gctgcgcgcg ccggccagca atgggccagc tgcacgaggc
     2881 ggtgccgcga cagggctcga aacgccgacg attcgcactc tcatcttcaa cacgctttgc
     2941 acctggtccg ggttacgatt tcgaagtcgc aaacaaggct gacgccgacc gtgaatatcc
     3001 ggctaaccga aggcctggat tccggtctgc gcgcgcccca ggatcagggc gtgaatatcg
     3061 tgcgtgccct cgtaggtgtt gaccgcctcg aggttcatca ggtgacgaat cacgtggtat
     3121 tcgtcggcga cgccgttgcc gccgtgcata tcacgcgcca gccgcgcgat gtcgagcgcc
     3181 ttgccgcaat tgttgcgttt catcagcgat accagttcca ccgggcagtt gtcgtcatcc
     3241 atcagccgcc ccatgcgcag gcagccctgc agcccgaggg tgatctcggt ctgcatgtcg
     3301 gcgagctttt tctgcaccag ctgggtggcc gccagcggtc gtccgaactg gctgcggtcg
     3361 agcgtgtact ggcgcgcggc gtgccagcag aactcggccg cgcccatcga gccccaggcg
     3421 atgccgaaac gcgcccgcgt caggcagccg aacggccctt tcagcccctc gacattgggc
     3481 aacaggtttg cctccggcac gaatacgtcc tgcatcgcga tttcaccggt gaccgaggca
     3541 cgcaacgaaa acttaccctc gatcttcggc gtcgaaagcc cgtccatatc gcgttcgagg
     3601 atgaaaccgc gaatcacgcc atcgagcttc gcccagacca tgaacacgtc ggcgatcggc
     3661 gaattggtga tccacatctt ggcgcccttc agccggtagc cgccgtcgac cgcggtggcc
     3721 gtggttttca tgctcgccgg gtcggaaccg gcgtcgggct cggtgagacc gaaacagccg
     3781 accagttcgc cgcgggccag gcccggcaga tacttgtgtc gctgctcgtc gctgccatag
     3841 gcataaatcg gatacatgac cagcgacgat tgcacgctca tcgccgagcg atagccgctg
     3901 tcgacgcgct cgacctcgcg cgccaccagc ccgtatgaga cgtagttagc gccggcgcag
     3961 ccgtattctt ccgggagcgt cgcacccagc agcccgagct cgccgaactc gttcatgatt
     4021 tcgcgatcga aaacttcgtc gcgatgcgcc tgcagcacgc gtggcagcaa ttgctgctgg
     4081 cagtactcgc gggtggcatc gcgaatcatg cgctcgtcct cgtcgagctg ctgctcgaga
     4141 aacatcgggt cctgccagtc gaaggaaact cgttttgctt gtgtcattac gaatcggggg
     4201 gaatttgtgg ccgaagttcg aattttatcg acgcgccgcg atattgccag acaaattagc
     4261 cattgcgcgg ccgcaacgcc cgcatcagcg ccgatcgaca aattgcagcg cgagcgtgcc
     4321 gtagctgtaa ccggcggccc gcaagccgcg ccagctctcg cggtgcagcg gttgcaacgg
     4381 aaacgggaag tcctgctcgc tgccgccggc gatcagctcg gcgaggtgct taccgatcac
     4441 cgtacccggg ccgataccgc ggccgttata cccggccggc gcgtagactc ccggggccag
     4501 caattgcacg cgcagtattc tgctgtcggt gacgccgacc cggccgctcc agcgatattc
     4561 ccagcgcagg ccgcgggttt gcggaaacag gcgttcgcgg ttgcgccgca tccagtcctc
     4621 gcgcaccggg cgctggtaac cggcgagctg cccggcgctg ctcatgatca atcggccggc
     4681 gccgtcgata cgcgacgaag tcatcaacgg ctgcgtatcc cacaggccgt gccgttcggg
     4741 aatcagttcc gcagcgagtt cgggattcag cggttcgctg gcgcactgga agatatacac
     4801 cggtacggtc gcttcgcgga cccgagagct gttggcatcc gcgtaggcgt tggtggccag
     4861 gatcacccgc tcggcgctga cctcgccggc ggcggtggtc gcgcgccagc cctgtgccgt
     4921 ctgctcgagc ccgaggatcg ccgattcctc gaagatgcgc gctccggcag ccagcgccgc
     4981 gtgcgccagc gagcgcacat aagcgagcgg gtggatggta ccggcgccgg gatcgacgat
     5041 gccgccgtga cgatatagcg gcgaacccga caatcgcgcc gcggtcgatg cgtcgatcat
     5101 ttccaccgcg gcgccgagtc cctgaatctg gcggcaacgt tcctcgagat ccttcagcgc
     5161 cgcggcactg tgcgcgatct gtatcgtgcc gacacggcgc gcgtcgcatt ccatcgcgtg
     5221 gcgttcgacc agcccgaaaa ccaacgccgg cgagtcgcgc agcgcgaggt tgaagcgctc
     5281 ggccctggcg gtgccgagtt gctcgaccac gtggcgcggg ttgcgccaga cgccggcatt
     5341 gaccagcccg gcattgcgcc ccgagccacc gtaaccgagc ctgcggccct cgagcagcac
     5401 cacgtcgacg ccggctgcag cgagatgcag cgcgctcgac aggcccgtat aaccgccgcc
     5461 gaccaccagc acgtcggcgc gcacggcttc ccgcagccgc tcgcaggccg gcgcggcgat
     5521 cgcggtggcg gcccacagcg agttttcgcc gggatcctgt ggctggtggt cgggcatatg
     5581 caggcttcgt gaataacgca tgaattgtgc gcgatccacc gttttattac cagccggccc
     5641 gatcgaaatc gccaccaata gacaccctgc atttactgtc ggaaccagcc cgagcgacag
     5701 cgaacccggc gatctcgtca cgccatccgt cgagccgctg cccgaagatg ccgacgctcg
     5761 tcgaacgcgc tgccaacggc gggccgagcg cggtacaatc caccgcacgc agagacgaca
     5821 agccgcgcgt gctcggcgac tcgatcaacg ccctgccatc ggagactgac catgctcacc
     5881 gtctatagcg aaaaacatgc cctgcgcgat gccgcgaccg aactctacgg cggccggctg
     5941 gtgccgccct tcgaatgccc ggtacgcgcc gaacacgtgc tcgcccgcgt gcgcgaggtc
     6001 gggctcggcg aaatcgtcac gccgcgcgag ttcggcatcg atgcgatcac ccgcatccac
     6061 gacgcggact tcattcgttt tctcgaaact tgctggcagg actggaccgc ggccggctac
     6121 gagggcgaag ccataccgaa ttgctggccg gcacgcggca tgcagcagca acgcgtgccc
     6181 cggcatatcg aaggcaagct cgggcattac gcgatggccg cagaaacctc gatttccggc
     6241 ggcacctggg aagccgcgcg cgccagcgcc gacgtcgcgc tgaccgccca ggccgcgctc
     6301 gccgacggcg cccgcgaggc tttcgcgctg tgccggccgc ccggccatca cgccgcggca
     6361 agcctgttcg gcggctactg tttcatcaac aacgccgccg tcgcggcgca ggcgttcctc
     6421 gaccagggcg cctcgcgggt ggcgctgctc gatgtcgatt tccatcacgg caacggcagc
     6481 caggcgattt tctaccgccg cgaagacgtc atgttcctgt cgctgcacgg cgaccccgac
     6541 gacgcctttc cgcattttct cggctacgcc gacgaaaccg gcgaaggcgc cggtgccggc
     6601 ttcaatcaca actacccgat ggggcccggc accggcttcg atacctgggg cgcggcgctc
     6661 gacgatgcct gccgcaagat cgccggctat gctcccgatg cgctggtggt ctcgctcggc
     6721 gtcgatacct tcgagcgcga tccgatttcc tttttcaggc tcggcagcga agacttcact
     6781 cgctacggcg ccaccatcgg ccgtctcggt ctgccgacgc tgttcctgat ggaaggcggc
     6841 tacgcggtcg aggaaatcgg tatcaacgcg gtcaacgtgc tgcagggttt cgaggggcga
     6901 taagcgccgc acaatgaagc ggctcgccgg tctcggtccg aatacccgcg gcgtactctg
     6961 cctggtgatc gcgatcgcct ggctgacggt cagcgacgcg atcatcaagt ggttgagccc
     7021 cgagctgccg ctgcaccaga tcatgctgct gcgttcgctc gtcgccctgc cgctggtact
     7081 gctgatcgct cacctcgagg gtggctggcg cacgctgaaa acgcagcgcc cgctcgccca
     7141 cttgctgcgc ggctcgctgc tggtgctggc caacatgtgc ttctttaccg cgctcgcggc
     7201 gatgccgctg gcggaaaccg tgacgctgtt ttacaccgca ccgctgttca tctgcctgct
     7261 gtcctggccg ctgctcggcg aacgcgtcgg cgccgcgcgc tggatcgcga tcgccgtcgg
     7321 catggccggc gtggtgatca tgatgcagcc cggagccgag ctgtttcgcc cggtcagcgt
     7381 gctgccggta ctggccgcgc tcagctacgc ggcgatgacg atgatgacgc gcaagctcgg
     7441 tttacgtgaa accgcgggcg cgctgacctt ttacatccag attgcgttca tcgtcatcag
     7501 cgcaatcgtc gggctgctga tcggcgacgg cggtttcgat aacttcgaca atccgtcact
     7561 gcagttcctg ttgcgcgaat ggcgctggcc aaacgccatc gagtcgcaat tgctgatcgc
     7621 ctgtggcgcc atcgtcacca tcggcggcta cctgatctcg caggcttacc gcatcggcga
     7681 ggcgcccgcg gtagcgccgt tcgagtatgc ctcgctgcct ttcgcgctgg cggtcggctt
     7741 cgcgctatgg ggcgactggc cgaacgcgcg cgcttttctc ggtaccggct tgatcgtcgg
     7801 cagcgggctg gtggtggttt acctggaaaa ccgcgcctat cgcaagtctt tgcgacccgc
     7861 accgatcgac ctgtgatttg tcctggatca atgcgctgcg gcgccacagg ccgaatactt
     7921 gtcatgcttg ccgcaggcgg tgagcgaatc ggcttgaccg gagagacgag tgaccgaagc
     7981 agaccgactc agccagcgac tgctggatat gaaaagcgag ctggagcggc gcctgcgttc
     8041 gatcgatcgc gacattcgcc acgagggcat gtccgccgac tggagtgaac aggcgagcga
     8101 gcgcgaaaac gacgaggtac tcgaatctct cggcagcaac tcggaaaagg aactgcgcat
     8161 gatcgaacag gcattgcgcc gaatcgacga cggcagttat tttcgctgcg acaattgcgg
     8221 cgaggaaatt cccgaggcca ggctggagct gctgcccttt accccctatt gcgtcaactg
     8281 cgcagagcaa ttgcaaccct gaggccttat ccatgctgac cgacaaccac gacctgctac
     8341 acgaatttcc ggagtaccgc gacacgattc atcagctgaa actgggcaac gcccatttct
     8401 cgcggttgtt cgacgagtat cacgagatcg accacgaggt gcaccgcatc gagaccggcg
     8461 tcgaaaatac ctcggacgag tacctcgagg aacgcaagaa aatgcggctc tacctgaagg
     8521 acgaactcta ccgcatgatc agggagactt aatccaccga cctgctgtcc ggcaggtagc
     8581 agacgaccgt gcagccgtgc tcgctgtaag tcgtgtgctg ggttcccgat ttcagcaaca
     8641 ccagatcgcc ggggcggtaa accgtgccgt cgttatcgac cgcatcgccg ctgatgacat
     8701 agaactcctc cgctcccaga tgctcgtggg gcagcgtcgt gtaccccgga tccatgcggt
     8761 agatatgaaa gccggtgccc gccggtttgc tcttattcaa ctgcaacacc cagccgtcct
     8821 gcttaccctg gtcgttgatg aagggctcga attcggcctc gtcgatattg gcgatcaaac
     8881 gctcgctgtc ggctatcggt ttcatgctaa tcctcggtga cggtttcggg tatcaccgcg
     8941 atcacgtcga tttccatcag ccattcgggc tgcccgaggc cggcgaccac cagcccggtg
     9001 gaaatcgggt agacgccctt cagccacttg ccgacttcct ggtagacggg ttcacggtaa
     9061 cggggatcgg tgatataggt cgtggtcttg acgatatggg acaggtcgct gccggcctcg
     9121 tgcagcaact ggtgcacgtt cttcatcgcc tgctcggcct gtgcgcgcgg atcgccgaca
     9181 ccgaccagct tgccgtcgaa atcggtgccg acctggccgc gcaggtatac ggtgttaccg
     9241 gcgaccaccg cctggcacag atcgttgtcg agcgcctggt tgggataggt atcgcgcgtg
     9301 ttgaacatgc gaatgcgttt gtgagtcggc atcgtgtgat ccccgtggca gatgacgcga
     9361 atagtagcag aacgaagcgg tgcttccagt gcgcaacatc gcccacggaa tgccacaggg
     9421 tttactcgag cggcccgcta agttcatccc cagcccgcga tccgccggtc ttcgagtgcg
     9481 aatttcgatg cgctgtttcg caggcgctcg agtcctgcat cccggctcag cgctatcagt
     9541 gatttatttg ccctgctcca gcacttatac tgttgcgcca taaagcgatg ccggacgaga
     9601 cgcgccgcgc gcagtgacga taatcccagc ctatgcccga taacagttcc gcagcgccga
     9661 tcgagacgat agatacgctg gtggtcggcg gcggccaggc cggtatcgcg atcagcgaac
     9721 acctcggccg ttgcgctatc ccccacctgg tcgtggaacg cgaccgcatc gccgaacgct
     9781 ggcgcagcgg ccgctgggat tcgctggtcg ccaatggtcc ggcctggcac gaccggtttc
     9841 cgggcatgga atttaccggc ctcgacgccg acgccttcgc gcccaaggaa atggtcgccg
     9901 attatttcgt cgcctacgcg gaaatgatcg ccgcgccaat ccgctgcggc gtggaggtga
     9961 cgcgcgcgca acggcaggcc ggcggcgctg gctttcgcgt cgaaaccacc gccggggtaa
    10021 tcgagaccga caacctggtc gccgccaccg ggcctttcca gcaaccggtg ataccgcccg
    10081 tggtgcccgc cgacccgtcg atccggcagc tgcactcgag cgactatcgt aaccccggcc
    10141 agctgccggc gggcgcggtg ctggtggtcg gcgccggttc ctccggcgtg cagattgccg
    10201 aagaactgat gcattcgggc aggcaggtct atctttccgt cggcccgcac gatcggccgc
    10261 cgcgcgccta tcgcggccgc gacttcgtct ggtggctggg cattctcggt aaatgggacg
    10321 cggtggcgct cgatcccggc accgagcacg tcaccatcgc ggtcagcggc gcgcacggcg
    10381 gccacacggt cgacttcaag cgcctggcgg cgcagggaat gacgctggtc ggcatgacgc
    10441 aatcctgccg cgacggcgtg cttcggttcg cgcccgacct ggcgcgcaat atcgcgcgcg
    10501 gcgatgccaa ctacctgtcg gtgctggacg aggccgacga ctacgccgag cgctacgggc
    10561 tcgatctgcc caccgatccc gcggcgcgcg aactcgagcc cgatccgccg tgcgtcaccg
    10621 acccgctgct cgaattgaac ctggccgagg ccggcgtcac tgcgatcgtc tgggcgaccg
    10681 gcttcaccac cgacttcggc tggctgcagg tcgaggcctt cgacgagcac ggcaagccgc
    10741 ggcatcagcg cggcattggc gccgcgcccg ggctttattt cctcggtctg ccgtggctgt
    10801 cgcaccgcgg ctcgtcgttc atctggggcg tctggcacga tgccgcattc atcgccgatc
    10861 atatcgccac ccggcgccgt taccgcggct accggccggc accggccgct gttgccaacg
    10921 ccgcgcaaga cgcccatggc gacggctgaa cctgccgcga tggtgaaggc tatcgccaca
    10981 ttccggcccg gactgcgctg tttcgcagcg gctatcgaca gccggcgcag cggcgatgcg
    11041 caatcgctaa ccgaggagtc gacatgaccc tgtcaatcgc cgcctgggac gagaccagcg
    11101 gccagctcgg cgcggcgatc gcgtcgtcga gtatcagcgt cgccgcgcgt tgcctgcact
    11161 ggcggcccgg catcggcgtc gcgctcagcc agaacatcac cgacccgcgg ctcgggccgc
    11221 gcctgctggc gctgctcgcc gacggcgtca gcgtggccga tgcgctcgcg gcagtcggca
    11281 ggagtacgcc tcatatcgcc taccggcagc tggtggtggt cgacagtacc ggcaacaccg
    11341 ggctgttcac cggtgaccag gcgctcggcc gcttcgccgc cgccgagggc cggcattgcg
    11401 cggccgccgg caacctgctg gccgacgcag cggtgccggg cgcgatgacc gcggctttcg
    11461 aaaatgccgc cggcagcctc ggcactcgcc tgctggccgc gctgtgcgcc ggcgccgatg
    11521 ccggcggcga agccggcccg ctgcactcgg ccggcctgct ggtcggcggc gaacccggct
    11581 ggccgttggc cgagctgcgc atcgactgga gcgacgaccc gctcgacgcg ctgcgcaccg
    11641 gctgggcggt ttacgaaccg cagatcgacg actacgtgac gcgcgcctac gatccgacac
    11701 gggcaccctc ttacggcgtg ccgggagacc cttgaatgaa cagcgaacag attctcgcgc
    11761 ggctggtcgc gttcgacacc accagcacgc gctccaacct cgagctgatc gatttcgtgc
    11821 gcaacctgct cgacgagcat ggcattgcct cgcaactgct gcacaacgac gagcaaacct
    11881 gcgccaacct ctacgccacc atcggccccg atgaccgcgg cggcatcatg ctgtccggcc
    11941 acaccgacgt cgtaccgacc gccggccagg actggcacag cgatccgtat cgcatgaaaa
    12001 ccggcgatga ccgcctttac gggcgcggct gctgcgacat gaagggcttc atcgcctgca
    12061 cgctggcggc gctgccggaa ctgacggcca ggccgctgca aacaccggtg cacctcgcct
    12121 tctcctacga cgaggaaatc ggctgcgtcg gcgccaagcg gctggtggcg gcgatgtcgg
    12181 gtttcgaggt caggccgcgg ctcggcctga tcggcgagcc caccagcatg cagctggtgc
    12241 tcggccacaa gggtaaagtt gcctaccggg tcaccgtcag cggcctgtcc tgccactcgg
    12301 cctatatcga ccagggcgtc aacgcggtcg agtacgcggc cgaactgatc gccttcattc
    12361 gagcgatgaa cgagcgtgcg cgcgcgcgcc tcagcgaccc ggcttactcg gtgccgcaca
    12421 gcacttttca cgtcggcaat atcgacggcg gcacggcact caacatcgtg ccgcggcaat
    12481 gccactttga attcgagatt cgcaacctgc cgcagcaaga cctcgatgag ctggcacacg
    12541 agatccgcca ttacgccgag gatacgctgc tgcccgagat gcgctcgcgc tacccgcaaa
    12601 gcactatcga attcgaagaa atttcctcct acccgggact acacaccgac ccggcggcgg
    12661 gcgtgatcac tcgcgttcgc gaactcaacc cgctcgccga gatcggcgat aacgtcagct
    12721 tcggcaccga ggccggcctg ttcgacagcc tcggcatcga gtgcctggtc tgcggcccgg
    12781 gcagcatcga gcaggcgcac aagcccgacg aattcgtcgg ccgcgcgcaa ttgcagcact
    12841 gcgatcgcat gctggaaaac cttgcgcagc actgccgcga gacggcagaa tccgcgtaac
    12901 actcgacgag cgatcgactt gatgtccgca gacaaagata agctctgtat cggctggcgc
    12961 gaatgggtcg cgctgccgga tctcaacctg cccgcgatca aggccaaggt cgataccggc
    13021 gcgcgcacct cggcgctgca cgcgttcctg atcgagcctt atacgcgcga cggcacggac
    13081 atgctgcgct tcatgattca cccgatccag aagaaccagg agctggccgt cgagtgccac
    13141 tgcccggtgt tcgactaccg ccaggtcacc gactccggcg gccatcgcga gatgcgctac
    13201 gtgattcaaa gccaggtggt gatcggcggc aagcaatggc cgatcgagct taccctgacc
    13261 aaccgtgata caatgcgctt tcgcatgctg ctggggcgcc gcgccatgga agaccgcttc
    13321 gtggtcgatc cgggcgcatc ctacgtcaac ggcagattga gaccccgcaa actctacgta
    13381 atccagcaag gataaccgca tgaaaatcgc cttactctcg cgcaaccgtc ggctctattc
    13441 ctcccggcgg atcatcgaag ccgccgagaa gcgcggtcac gagatcgtcg tcatcgacgt
    13501 gctgcgcgcc tacatgaaca tcgccgcgca caaaccctcg atccactaca agggcgaggc
    13561 actggagggc ttcgatgccg tgattccacg catcggcgcg tcggtcacca actacggtac
    13621 cgcggtattg cgccaattcg agatgatggg cgtggtctcg ctgaacgagt cggtcgcgat
    13681 cacccgatcg cgcgacaagc tgcgttcgtt gcagttgctg tcgcggcgcg gcatcggcat
    13741 gccgctgacc ggctacgcca acaagcccga caacatcaag gatgtgatca agatggtcgg
    13801 cggtgcgccg gtggtggtca agctgctgaa gggcacccag ggcatcggcg tggtactggc
    13861 ggaaacgcaa aaggccgcgg aaagcgtgat cgagggtttc atgggcgtca aggccgatat
    13921 cctgatccag gagttcatca aggaagccgg cggctcggat attcgctgct tcgtcatcga
    13981 tggcaaggtg gccgcggcga tgaaacgcca gggcgccaag ggcgagttcc gctccaacct
    14041 gcatcgcggc ggtacctcga ccctgattcg catcaccccc gaggaacgct cgacagcggt
    14101 ccgtgcggcg aagatcatgg gcctcaacgt cgccggcgtc gacctgctgc gctccaatca
    14161 cggcccggtg gtaatggaag tgaactcgag cccggggctc gaaggcatcg agagttcgac
    14221 cggcaaggat atcgccggca tgatcatcac caatctcgag aagctcaagc ccggcaagac
    14281 ccgcacccgc ggcaagggct aatcggcaga tgtcatcgat attcgtcgcc agagtgtcgg
    14341 caaggcaccg tgccctgctc gatcggcagg cgccggcgtc cgcgccagcg gcccgatgac
    14401 ttatggcggc taaatcccag ggttcgatcg atatcgcggg tgtcaccgtc aagcccggca
    14461 accgccagtc gatcgatatc ccgctgccga gtttctatac ccacagcagc gtcaacatgc
    14521 ccgtgcacgt ggtgcgcggt cgcaagcccg gtccggtgct gctggtctgc gcgacgattc
    14581 atggcgatga aatcaccggc gtcgaaatca ttcgccgcct gttgacccac aagttcatca
    14641 accgcattcg cggcacgctg atcgcgatcc cggtggtcaa tgtctacggt ttcgtctcca
    14701 agtcacgcta cctgcccgac cgccgcgacc tgaatcgctc gtttcccggt tccgagagcg
    14761 gttcgatggc atcgcgcctg gcgcacgtac tgatgaccga aatcctgccg cactgcaccc
    14821 acatcgtcga cctgcacagc ggcgcggtca accgcgaaaa cctgccgcag attcgcgcca
    14881 agctgaagaa cgaacccgag ctcgaagcgc tggcgcatgc ctttggcgtg ccggtaatac
    14941 tggacatgaa cctggtcgag ggctcgctgc gagaagccgc ctacgagcgc gacattccga
    15001 tactggtcta cgaggctggc gaagcgctgc gcttcaacga ggtcgcgatc cgcgccggcg
    15061 tgaaaggcgt gctgaacgtg atggcggcgc tcgacatgcg cctgagaaag cgcaaggcaa
    15121 gcaagacgcc ggcgatgatc gccaacgcca gccgctgggt gcgcgcgccg caaagcggca
    15181 tcctgcgctc gctggttacg atcggcgcac gggtcgcggt cggcgacgtt ctcgcctaca
    15241 tcaatgaccc gctcggcgaa aacaacaacg agatcgtcag cgaactcgaa ggcatcgtca
    15301 tcggcagatc caacctgccg ctggtgtttg ccggcgaagc ggtcttcaat atcgcgagct
    15361 atgcctcggt cgagccggtt tccgatcgca tcgaggctta ccacgaggaa ctggcgccga
    15421 acggcgacgt gccgaacaac gaggaaccgc cgctggtcta gccgctggct ttcgccgccc
    15481 agctcgatgc gccgagcgct gctcgcctgg gtccgcagca tatcgcgaac cgaactattg
    15541 ccgccgcttt ttcggctcta cagccgcagt ggaactgtcg cccgggcaaa tacagccagg
    15601 ccgcgagccg actcgcgaca cctgtccgct tcgcgatcgc gatggctgtc aggcggcgct
    15661 cgccgcgggg cgctcgaccg gttcctcgcg aatcggccag tgcaccaccg ccgcgaacac
    15721 accgagcgcg acacccagcc accagatgac gtcgtaggaa ccggttgcgt cgtagagcac
    15781 gccgcccagc cacacgccga gaaacgatcc cacctggtgc gagaggaaca cgataccgcc
    15841 gagcatgccg aggtagcggg tgccgaaaat gaccgccacc agcgcgttgg tcggcgccac
    15901 ggtagacagc cagagaaggc ccatgacgat cgaaaatgcg accaccgtag ccgggctcgc
    15961 cgggatcagc aggaacaggg tcaccagcac cgagcgcagc aggtagatcc acaccagcag
    16021 catcggcttg ggataacgct gcccgatgat acccgatgcc agcgaaccga taatattgaa
    16081 cagcccgatc aggccgatag cgatcaccgc ccagcgcgcg gcg
//
LOCUS       DATCZV010000376         3584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_719118, whole genome shotgun sequence.
ACCESSION   DATCZV010000376 DATCZV010000000
VERSION     DATCZV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719118"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIW27_08465"
     CDS             <1..191
                     /locus_tag="VIW27_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739746.1"
                     /translation="CLLIDATRVDAGQAAARIAAFERHLQPARLNFAGPRASEAPAAY
                     AYTLQAVGKYLAQRGPGD"
     gene            307..699
                     /locus_tag="VIW27_08470"
     CDS             307..699
                     /locus_tag="VIW27_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY5739747.1"
                     /translation="MNQGARTGGAVSPRIEHLEWGQMEIAGIGGGRDFKLWPGGGREW
                     DWRETGTLHEPGIQPADVAELVAAGCRAIVLSRGMLLKLHTCPETIELLEHEGIEVHV
                     AQTREAAEIYNALVSRGIAVGGLFHSTC"
     gene            730..1023
                     /locus_tag="VIW27_08475"
     CDS             730..1023
                     /locus_tag="VIW27_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739748.1"
                     /translation="MTGPRANNRCPNCGHRFSDLEMLLWGMSKTGKCPDCQVELGVNS
                     NRVLMLWFAGIIAILFIEQRFSLDSYHGWLAIAAFVLVFCLVAIRVQKLEIRE"
     gene            1111..1323
                     /locus_tag="VIW27_08480"
     CDS             1111..1323
                     /locus_tag="VIW27_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEY5739749.1"
                     /translation="MAKSRQGKKLIGLVLTVVGGGLAIWGYRMSGSFGSRLNEALSGS
                     YGDKVMLLYIGGAACIVVGLLLLLKK"
     gene            1398..1667
                     /locus_tag="VIW27_08485"
                     /pseudo
     CDS             1398..1667
                     /locus_tag="VIW27_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008303418.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1755..2618
                     /locus_tag="VIW27_08490"
     CDS             1755..2618
                     /locus_tag="VIW27_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5739750.1"
                     /translation="MLRTSQSRVKGMSVNRLIEDRFGLESEIGRSIDDNGTLGGILAR
                     RSCRTFSGDEVPRDLLDTLLAAAFSAPSKSDLQQACVIHVNDRDRQRRIAGMKDSTRW
                     VADAPLLLVWCGDSRRIRRLAEWRGHAFANDHLDAFMNAAVDAGICMQTFIVAAESVG
                     LGCCPISEIRDHIEPLSAELGLPQHVFPVAGLAVGWPRGDRRLSLRLPMSVTVHEDRY
                     DDSGLFEIVAEYDRRREALEQTPPQRQRLIDEFGVASDYGWSENRTRQYSQPARADFG
                     RYIRKQGFDLD"
     gene            complement(2631..3119)
                     /locus_tag="VIW27_08495"
     CDS             complement(2631..3119)
                     /locus_tag="VIW27_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739751.1"
                     /translation="MRRLMIIAAILLPAWGTTGAAPVDARAAWDNRCEECHGEADKFS
                     RKYLWAIGGRLQGRHHVLDLRLFMSNHYIPNHMLDMMQAMLMEDANTPQRFADECSSC
                     HGEVEDFVGKSVAVLWGKVRGIESGIAVSEFLATHQGLQEEDVEFFTHLLERFAELLQ
                     QP"
     gene            complement(3345..>3584)
                     /locus_tag="VIW27_08500"
     CDS             complement(3345..>3584)
                     /locus_tag="VIW27_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739752.1"
                     /translation="GDGSEEKIVNGEELGWRPYIEPRSVEISASTDCGLIVIEAGTYQ
                     KLLRTTPQLNYQTRKRLSIETDDKADWLLEDVEIY"
BASE COUNT          628 a         1085 c         1188 g          683 t
ORIGIN      
        1 cctgcctgct gatcgacgcg acgcgggtcg atgccgggca ggcggccgcg cgtatcgccg
       61 ccttcgagcg gcacctgcag ccggcgaggc tgaacttcgc cgggccgcgc gccagcgagg
      121 cgcccgccgc ctacgcgtat acgctgcaag cggtcggcaa atatcttgcg cagcggggcc
      181 ccggcgattg acgccggcgg tcgcgcggta ccgggagttg gcggcattcg tgttagaatc
      241 gacgttgtct cggctgttat tggtcgcggc ggatcgaaac ttgtcgcgcg aggcgcggag
      301 gcaaacatga atcagggtgc gagaacgggc ggcgcggttt ccccgcgtat cgagcatctc
      361 gagtgggggc agatggaaat tgccggtatc ggcggcggca gggatttcaa gctgtggccg
      421 ggcggcggtc gggaatggga ctggcgcgag accggcaccc tccacgagcc gggtatccag
      481 ccggccgacg tcgccgagct ggtcgccgcc ggctgccgcg cgatcgtgct cagccgcggc
      541 atgttactga aactgcacac ctgcccggag acaatcgaac tgctcgagca cgagggtatc
      601 gaggtgcacg tcgcgcagac ccgcgaggcc gccgaaatct ataacgcgct ggtgtcgcgt
      661 ggtattgccg tcggcggcct gtttcactcc acctgttgac gtgccgccct ggcgcgcgcc
      721 aaacgaacca tgaccggccc gcgggccaac aaccgctgcc cgaactgcgg ccaccggttc
      781 agcgatctcg agatgctgct gtgggggatg tcgaagaccg gtaagtgccc cgactgccag
      841 gtcgaactcg gcgtcaatag caaccgggtg ctgatgctat ggtttgccgg tatcatcgcg
      901 atcctgttca tcgagcagcg cttttcactg gatagttacc atggctggct ggcgatcgcc
      961 gccttcgtgc tggtgttttg cctggttgcg attcgcgtgc aaaagctcga gatcagggag
     1021 tgagtcattg cccggtcgag aatggcggaa ggccgggctg ctgctataaa caaccctgtg
     1081 acgagcatca attttccccg ggagtaaaac atggcgaaat ccaggcaggg caaaaaactg
     1141 atcgggctgg tcctcacggt ggtcggcggc ggtctcgcga tctggggtta ccgtatgtcc
     1201 ggatctttcg gctcgcggtt gaacgaggcg ctgtccggct cgtatggcga caaggtcatg
     1261 ttgctgtaca tcggcggcgc ggcctgcatc gttgtcggcc tgttgctgct gttgaagaag
     1321 tagccggcag tcgcctgccg agatcgaaaa cgcgctttat gactcggcgc accaggtgat
     1381 atcactcgaa atgactgcag ggcatcgtct acacccgcgg taattagcag gcgcagaccg
     1441 agtacctgtt tacccgcctc gatgaaatca agccatagat gatcgcggaa gccacgcgca
     1501 aggcgcgcga ggcggcggaa aattcgccgc cgattctgaa gagcacgctc ggcaagatca
     1561 aggccgcctc gccggggcag ttcacgatca gcgaccgcga caaggacaat ccgcatatca
     1621 agaagatccg tgtcgtttcg acgttagctt actatctctg cgactgatgg ccggcgattt
     1681 cgcgggacat gatgccagga tcggcaatga ctcgaggggt gtgcgcggca ttgcaggcgc
     1741 agtcgtttcg gtccatgctt cgaacatctc agtcacgggt caagggcatg agcgtcaacc
     1801 gcctgatcga ggaccgcttc ggcctcgaaa gcgagatcgg caggtcgatc gatgacaacg
     1861 gtacgctggg cggcattctc gcgcgccgca gttgccgcac cttcagtggc gacgaggtgc
     1921 cgcgggatct gctcgacacg ttgctggctg ccgcgttttc ggcaccgtcg aaatcagacc
     1981 tgcagcaagc ctgtgtgatt catgtcaacg accgtgaccg gcaacggcgc atcgccggga
     2041 tgaaagactc gacgcgctgg gtcgccgacg cgccgctgtt gctggtgtgg tgcggcgaca
     2101 gccggcgcat tcgccgtctc gccgagtggc gcggacacgc ttttgccaac gatcatctcg
     2161 acgcgttcat gaatgccgcg gtggatgccg gcatctgcat gcaaaccttc atcgtcgccg
     2221 ccgaatcggt gggactcggc tgttgtccga tcagcgagat tcgcgaccat atcgagccgc
     2281 tgtccgcgga gctgggattg ccgcagcacg tgttcccggt agccggcctc gcggtcggct
     2341 ggccgcgagg cgacaggcgc ctgagcctgc ggctgccgat gtcggtcacc gtgcacgagg
     2401 accgctacga cgacagcggc ctgttcgaaa tcgtagcgga atacgatcgg cgccgcgaag
     2461 cgctcgagca gacgccgccg caacgccagc ggctgatcga cgaattcggc gtggcgagcg
     2521 attacggctg gtccgaaaac cgcacccggc agtattcgca gccggcccgc gccgacttcg
     2581 gccgctatat tcgcaagcag ggcttcgatc tcgactgagg gtcggcgctg tcagggctgt
     2641 tgcaagagtt ccgcaaagcg ttccagcaag tgagtgaaaa attccacgtc ctcttcctgc
     2701 aggccctggt gggtagccag gaattcgctg accgcgatcc cggattcgat gcccctgacc
     2761 ttgccccaga ggacggcgac cgatttgcca acgaagtctt ccacctcgcc gtggcaactg
     2821 ctgcattcgt cggcgaatct ctgcggcgtg ttggcgtctt ccatgagcat tgcctgcatc
     2881 atgtcgagca tgtggttcgg tatatagtgg ttgctcatga acaggcgcag gtcgagcacg
     2941 tggtgccggc cctgcaggcg gccgccgatt gcccacaggt acttgcgcga gaatttgtcg
     3001 gcttcaccgt ggcattcttc gcagcggttg tcccaggccg cgcgcgcatc caccggggcg
     3061 gccccggtgg tgccccaggc cggcagcagg attgcggcga tgatcatgag tcggcgcatt
     3121 atgcgtacct cgatggcgcc gtcgcaggcg cacgccagtg gtcaaaacga tgcgctccgc
     3181 ccgcgtcgac aacgcagcgc atgccgtcgg cttcgcagtt tacacaagat tggccggcgc
     3241 gtgggacgca agccggccaa tcggcggctg tgaaaccgtg gtttgcaagt gtcgcgcgga
     3301 aaaccatccg ccggctcgag gcagccgtgg gggcagcgcg ttcgctagta gatttctaca
     3361 tcctcgagca gccagtcggc cttgtcgtcg gtctcgatgg atagccgctt gcgggtctgg
     3421 tagttgagtt gcggggtggt cctcagtaac ttctggtagg tgccggcctc gatcacgatc
     3481 agtccgcagt cggtgctggc cgaaatttcg acgctgcgcg gttcgatgta gggacgccag
     3541 ccgagttcct cgccgttgac gatcttttcc tcactgccgt cgcc
//
LOCUS       DATCZV010000377         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_507289, whole genome shotgun sequence.
ACCESSION   DATCZV010000377 DATCZV010000000
VERSION     DATCZV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507289"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VIW27_08505"
     CDS             complement(<1..815)
                     /locus_tag="VIW27_08505"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY5739753.1"
                     /translation="MASEPQFTTGIEEEYLLVDLESGELVDAAPPGLVEDCDDAFPDQ
                     VHPELLQCQIEIGTRPRATMSELRSDLVDLRSIVSEQASRHGVGIIAASTHPWAIWRN
                     QRYTVQERYQKLGQRMQAVMQRMTICGMHVHVAIEPDSLRIDLMNQACYVLPHLLALS
                     TSSPFLEGRDTGLKSFRIALYAELPRTGLPEHFDNEADYQRAVDVLVDTGVIEDASRI
                     WWDIRPSIRYPTLEMRICDMCTRVDDAICVAALYRCWLRMLWRLRKKNQRWRT"
     gene            complement(861..2072)
                     /locus_tag="VIW27_08510"
     CDS             complement(861..2072)
                     /locus_tag="VIW27_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY5739754.1"
                     /translation="MKDFPDMHNMQQPINIEHGAGRPAEPSVASRLADFEADDLGLPS
                     WNALRKMLTAGPELAHLFPLVEPGIAVFAWRGEANEVRLRHFMSRDNVGFAFEREPGG
                     DLWRLRLRVPDRTRFEYKFDIDGHWMQDPLNPAQATDPFGANSVCETFGYSAPDWIHA
                     RPGVETGSLEETAIHSEAFGEQRRLGIYRPAGYQEHGDYPLVVIHDGFDYVDHAALLS
                     ILDNLIAAGDLPPLVAALTQSPNRMVEYVDDSRHAEFITRDLLPAVESACAVERSPQR
                     RVLMGASLGAVASLSVAARAPQVFDGVILKSGSFIFDPRLLQTRARLFRSIMQFIDGL
                     SREERLMHRAFVCCGRFEGLVGENRRMAAFLRRAGVATRYVESRDAHHWHNWRDQLRA
                     GLMWCLRHRYL"
     gene            2492..3916
                     /gene="glgA"
                     /locus_tag="VIW27_08515"
     CDS             2492..3916
                     /gene="glgA"
                     /locus_tag="VIW27_08515"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY5739755.1"
                     /translation="MWILHVAAEVHPLIKTGGLADVVGALPAAQRALGHEVAVLMPGY
                     PAVWKKAGKLEPIDEFDAPGGRRARLLRGKLAGDVDLLVIDQHELYGRPGNPYTDSDG
                     RGWPDNHLRFALLGRVAAWLCRRFPQTEVLHAHDWHAGLAPAYLRADGTRCASCFTIH
                     NLAYQGVYAHWEYAFTGLPEEFYRFDGLEFHGNWSFMKAALSFSDAITTVSPTYAREI
                     LRPELGMNLDSVLNYRRADLHGILNGVDYAIWNPATDVALNSGYSADRLAPKAEDKRA
                     LQQALGLDVAAERMLVGIVSRLTVQKGLDLVPQAIEGLLAADEAQLVLLGSGAHDLEQ
                     AFTELAARQPRNCAVRLGYDEDFAHRIIGGADTILVPSRFEPCGLTQLYGLRYGTLPI
                     VRKTGGLADTVTGYNPKTRGATGFVFETTSVLALQRILKRAIATYRDTPTWQGLMRNA
                     MRRDFGWQRAAQAYVDLYASIAGR"
BASE COUNT          671 a         1279 c         1320 g          661 t
ORIGIN      
        1 taggtgcgcc agcgctggtt ctttttacgc aaacgccaca gcatgcgcag ccagcaacgg
       61 tacagcgcgg cgacgcaaat cgcgtcgtcg acccgggtac acatgtcgca gatgcgcatt
      121 tccagggtcg ggtaacgaat gctcgggcgt atatcccacc agatgcggct ggcgtcctcg
      181 attacgccgg tgtcgaccag cacgtcgacc gcacgctggt aatcggcttc gttgtcgaaa
      241 tgctcgggca ggccggtgcg cggcagttcc gcgtagagcg cgatgcgaaa cgacttgagg
      301 ccggtatcgc ggccctccag gaatggcgac gaggtcgaca gcgccagcag gtgcggcagt
      361 acgtagcagg cctggttcat caggtcgatg cgcagcgaat cgggctcgat ggcgacgtgc
      421 acgtgcatgc cgcagatcgt catccgctgc attaccgcct gcatgcgctg gccgagtttc
      481 tggtagcgct cctgcaccgt gtagcgctgg ttgcgccaga tcgcccacgg gtgggtggag
      541 gcggcgatga tgccgacgcc gtggcggctt gcctgttcgc tgacgatgct gcgcaggtcg
      601 acgaggtcgc tgcgcagttc ggacattgtc gcgcgcggcc gagtgccgat ctcgatctgg
      661 cactgtagca gctcggggtg cacctggtcg ggaaaggcgt cgtcgcagtc ctcgaccagt
      721 cccggcggcg cggcgtccac cagttcgccg ctttccaggt cgaccagcag gtactcttcc
      781 tcgatgccgg tggtgaattg cggttcgctt gccatgtcct gttatccccc tgtcgatggc
      841 gcggcccgaa tctcagccgg ttacaggtat ctgtgccgca ggcaccacat tagccccgcg
      901 cgcagctggt cgcgccagtt gtgccagtga tgcgcgtcgc ggctctccac gtaacgcgtc
      961 gcgaccccgg cgcggcgcag aaaagccgcc atgcggcggt tttcgccgac caggccctcg
     1021 aagcgcccgc agcagacgaa ggcgcggtgc atcaggcgct cctcgcggct caggccgtcg
     1081 atgaactgca tgatactccg aaacagccgc gcgcgggttt gcagcagccg cgggtcgaag
     1141 atgaaggatc ccgatttcaa aatcacgccg tcgaatacct gcggtgcgcg cgcggcgacc
     1201 gacaggctgg cgaccgcgcc gaggctggca cccatcagca cgcgtcgttg cgggctgcgc
     1261 tcgaccgcgc aggccgattc gaccgccggc aataagtccc gggtgatgaa ctcggcatgg
     1321 cgcgaatcgt cgacgtactc gaccatgcgg ttgggcgact gcgtcagcgc cgcgaccagc
     1381 ggcggcaggt cgccggcggc tatcaggttg tcgagtatcg acagcagcgc ggcatggtct
     1441 acataatcga agccgtcgtg tatcaccacc agcgggtaat cgccgtgctc ttggtagccg
     1501 gccgggcggt agatgccaag ccggcgctgt tcgccgaaag cctcgctgtg aatggcggtc
     1561 tcctcgagac tgccggtttc gacgccgggg cgggcatgaa tccagtcggg cgccgagtag
     1621 ccgaaggttt cgcagaccga gttggcgccg aacggatcgg tggcctgcgc cgggttcagc
     1681 ggatcctgca tccagtggcc gtcgatgtcg aacttgtatt cgaagcgggt gcggtccggt
     1741 acccgcaggc gcaggcgcca gagatcgccg ccgggctcgc gctcgaatgc gaatcccacg
     1801 ttgtcacgcg acatgaaatg ccgcagccga acctcgttgg cctcgcctcg ccaggcgaac
     1861 acggcgatgc cgggctcgac cagcggaaac aggtgcgcca attccgggcc cgccgtcagc
     1921 attttgcgca acgcattcca cgacggcagg ccgaggtcgt cggcctcgaa atcggccagg
     1981 cgcgacgcga ccgagggctc ggcgggccgg ccggcgccat gctcgatgtt gatcggctgt
     2041 tgcatgttat gcatatcggg gaagtctttc actaccgggt gcggcaatac cctgagctaa
     2101 gcacagttta gggttcgctg caatctgtta cgacctcggc ctggcgtggt cggcagcgcc
     2161 gtgcttatcg agaaagaaat tgccgatctc gcaacccgcg gccagcctga aaaatatcga
     2221 ctcccggctg tagcgagcga cctgggcgca gccgcggaca gcgtcgaatc tggtaaactg
     2281 cgggcgtacg cggcgccgcg gttcgatgcc tgtcggagcg ggcgcgagcg ttgcggaggg
     2341 ggagaaatga acagtacatg ccgctattca cgacggccga atcaggggtg ggaaacgagc
     2401 gggtcgcaga caaatcacac gccaccggcg ctgatcgata tcatgccgcc gccgaggcgc
     2461 gtgatgcacc aggcctgagc attccatagc catgtggatc ctgcatgtag ccgcggaagt
     2521 tcatccgttg atcaagaccg gcggtctcgc cgacgtcgtc ggcgcgttgc cggcggcgca
     2581 gcgcgcgctg gggcacgagg tcgcggtgct gatgccggga tatcccgcgg tctggaagaa
     2641 agccggcaag ctcgagccga tcgacgagtt cgacgccccc ggcggccggc gcgcgcgtct
     2701 gctgcgcggc aagctcgccg gcgacgtcga cctgctggtc atcgaccagc acgaactcta
     2761 cggtcggccc ggcaacccct atacggacag cgacggccgc ggctggcccg acaaccattt
     2821 gcgtttcgcg ctgctcggcc gggtcgcggc ctggctgtgc cggcgctttc cgcaaaccga
     2881 ggtgctgcat gcgcacgact ggcacgccgg cctcgcgccg gcctacctgc gcgccgacgg
     2941 cacgcgttgc gcgagctgct tcacgattca caatctcgcc taccagggcg tttacgcgca
     3001 ctgggaatac gcctttaccg gcttgcccga ggagttctac cgcttcgacg gccttgagtt
     3061 tcacggcaac tggtcgttca tgaaggccgc gctgtcgttc agcgacgcga tcaccacggt
     3121 cagcccgacc tacgcgcgtg aaatcctgcg gcccgagctt ggcatgaacc tcgactcggt
     3181 actcaattat cggcgcgccg atctgcacgg catattgaac ggcgtcgatt acgcgatctg
     3241 gaaccctgca accgatgtcg cgcttaacag cggttactcg gcagacaggc tcgcgcccaa
     3301 ggcggaagac aagcgcgccc tgcaacaggc gctgggactc gacgtcgccg ccgaacgcat
     3361 gctggtcggc atcgtcagcc ggctgacggt gcaaaagggg ctcgatctgg tgccgcaggc
     3421 aatcgaaggc ctgctggccg ccgacgaggc gcagctggtg ctgctcggca gcggcgcgca
     3481 tgacctggag caggcgttca ccgagctggc cgcgcgccag cctcgcaact gcgccgtgcg
     3541 gctgggctac gacgaggact tcgcgcaccg catcatcggc ggcgccgaca cgatcctggt
     3601 gccgtcgcgc ttcgagccct gcggcctgac gcagctctac gggctgcgct atggcacgct
     3661 gccgatcgtg cgcaagaccg gcggcctcgc cgatacggtc accgggtaca acccgaagac
     3721 gcgcggcgcg accggcttcg tgttcgaaac caccagcgtc ctcgccttgc agcgaatcct
     3781 gaagcgggcg atcgccacct atcgcgatac gccgacctgg caagggctga tgcgcaacgc
     3841 gatgcgccgg gatttcggct ggcagcgcgc ggcgcaggcc tacgtcgact tgtacgcgag
     3901 tatcgcgggc cggtaattcg ccggcaagaa a
//
LOCUS       DATCZV010000378         8535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_613147, whole genome shotgun sequence.
ACCESSION   DATCZV010000378 DATCZV010000000
VERSION     DATCZV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613147"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VIW27_08520"
     CDS             complement(<1..454)
                     /locus_tag="VIW27_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004726237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5739756.1"
                     /translation="MSNSDWQARKEDAVARGQGNIAPVYVEHAKGSEIWDVEGKRYID
                     FGTGIAVCSAGHCHPQITAAVAEQLNRFSHTCVMVTPYTPVVELAERLNRAVPIADAR
                     TIFLSTGAEAVENAIKIARFKTGRPGVIAFSGAFHGRTNLCMGLTGKVV"
     gene            complement(490..1953)
                     /locus_tag="VIW27_08525"
     CDS             complement(490..1953)
                     /locus_tag="VIW27_08525"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5739757.1"
                     /translation="MSINISDPGLLKTQAYIDGKWVDADSGETVPVTNPANGEVIAAV
                     AKCSTAETRRAIEAAEAALPGWRSRTAKERAALMRKWFNLMMEAQEDLALIMTLEQGK
                     PLFESRGEIAYGANYIEWFAEEGKRIYGDTIPQPSNDKRIVCIKQPVGVVACITPWNF
                     PNAMLTRKIGPALAAGCTVVCKPANATPLSAYAFTELAERAGIPPGVINMVTGETSAI
                     GKELTSNPIVRKLTFTGSTPVGKQLMAECAQTVKRTSMELGGNAPFIVFDDADLDAAV
                     AGALVCKYRNAGQTCVCANRILVQESVYDAFAAKLADAVGGLKMGPGTDEDVKIGPLI
                     TEQAANEMEGFIEDALAKGAKMVAGGHRSDLGAHFFEPTILTNVSGEMRVFNEEIFGP
                     IAPLFKFKTEEEAIRMANDTEFGLACYFYSRDIGRIWRVAEALEYGIVGINEGIISNE
                     MAPFGGVKESGQGREGSKYGLDDYLEIKYMCLGGIDQ"
     gene            2130..4907
                     /locus_tag="VIW27_08530"
     CDS             2130..4907
                     /locus_tag="VIW27_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5739758.1"
                     /translation="MPDNASPNKGKARTNLDFYHSPTQLRFDTWHRIEENTSRLREKH
                     MRKADIAALSKKIRADLATIEVFESYSAFPSRDDVKLMWQLFDQQDYDHLARIVARIV
                     RALTNGTYRSTAIDLHSSIDVDDGDESLQNFDDGHEPRPYFELLFVDESGHEDIRRLR
                     ERLHELRRPEDNFVYDIVVVPSFEDALIAVLFNYNIQAVVIRYGFPLRSVNHLEILQR
                     YLSKIDESEYEDALDIERGPLLGNLISEMRPELDLYLVTDAAVEDIAGNVTQKFNRIF
                     YQQEDYLDLHLNVLRGIQARYETPFFTALRRYSRQPTGVFHAMPISRGKSVTRSHWIR
                     EMVQFYGINIFLAETSATSGGLDSLLQPHGPIKKAQELAARAFGARRTYWVTNGTSTA
                     NKIVVQALVQPGDIILIDRDCHKSHHYGMVLSGAHVCYLDSYPLDEYSMYGAVPLKEI
                     KRKLFEYKKAGKLHKVKMLLLTNCTFDGVVYNVERVMEECLAIKPDLIFLWDEAWFAF
                     AGFTPTYRQRTAMNTAARLRKRYASAEYRAEYEVYRKQISNASEADLLTERLMPDPDL
                     VRVRTYATQSTHKSLTSLRQGSMIHVYDQDFGFKVQEPFDEAFMTHTSTSPNYQILAS
                     LDMGRRQVELEGFEMVQKQVELAMTIRERVASHQLLSKYFRFLKPSEMIPAEFRTTGI
                     GSYFDPKKGWGSTEMENAWAEDEFVIDLTRLTLYLGLTGIDGDTFKNKFLMDKYGIQI
                     NKTSRNTVLFMTNIGTTRSSVAFLLEVLVKIATELDQERAEASRVERRLIDKRVKSLS
                     QDFPPLPDFSHFHAAFVPKSKSKIWTGEGDIRRAFFLAYDEENCEYMTIDEQTIGKAL
                     KSGRELVSASFVTPYPPGFPVLVPGQVISEQILAFIRALDVKEIHGYEPEIGLQLFTE
                     EALQKLQQSLP"
     gene            complement(5042..6088)
                     /locus_tag="VIW27_08535"
     CDS             complement(5042..6088)
                     /locus_tag="VIW27_08535"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY5739759.1"
                     /translation="MRFTFRAFGEDRPGPLWQKHFRRLWPGYRRWFLRYGERDRPTYF
                     ESVQALKKYIPEFLPCYEAMTDLAGGGDHAARFLAQYCPPPLFRGCSQAVYLQDESVL
                     VRNYDYSPYVFDGLVMKSRFEQRGVMAMLDCMSGALDGINSDGLAVSLSFGGREEFGT
                     GFGIPLLMRCLLEFAGNVGEATELLQRIPVHGAYNITLLDADDRFDTLAIGPGRQPQS
                     LGTPLATNHQPDSSWPIYEEKVATHLRYAHLAAIGEARQHSAFQFSREFLNTPLYSSK
                     FARGFGTLYSAAFYPRHRSCEYFWPHNSWGFGFDTFSDREYRIDFVDPEGYPRKSQTY
                     SEIYTGAPIPALQF"
     gene            complement(6107..6502)
                     /locus_tag="VIW27_08540"
     CDS             complement(6107..6502)
                     /locus_tag="VIW27_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739760.1"
                     /translation="MSQDVELGIVGSVTQADLAPLRTAGRLGETALVYRVERLFQGGG
                     YGDQDKHPLAWAVAVYVDSQPCRVFNARGAGREWNSIDRLAGWLREQGFWYWWTRNDL
                     EPVGSAMTEPEETALPASPAVLDPPFSPF"
     gene            6639..7211
                     /locus_tag="VIW27_08545"
     CDS             6639..7211
                     /locus_tag="VIW27_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5739761.1"
                     /translation="MSKKVSPIPRGYRTATPVLTVFDIDSAVGFYQAAFGAELLTRNA
                     AADNIAINASIKIGNSIVILNREAPQQGIFSPLSLGGSGGHTHLYVDDVNTSWERAIE
                     AGAQVRVALADTYWGDRSGVLVDGNGHLWSMASKIENVSQDEIARRAQALCEPAEAAA
                     PAAIYDFADAEYLPEPMPAEEVLAGDKVAV"
     gene            7282..>8535
                     /locus_tag="VIW27_08550"
     CDS             7282..>8535
                     /locus_tag="VIW27_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5739762.1"
                     /translation="MPEIALTATQQLKSLASGQISAVDLLEQTIARAEELGPLLNPIA
                     LKLYARARQAALEADRKRAAGKAGRLCGLPITIKDSQFLAGYPCANGSHVLSDFVPSE
                     TCRAIELLEQEGAVIFAKTTCPEFSLSGVTHSEIYGLTSNPWNPSRTCGGSSGGAAVA
                     VASGMGAFSLGGDGGGSIRIPAGFCGIVGFKPSFGMVPREPCFPSWQSIVAYGPMTRS
                     VEDARLFFDIIGENFTGNRNPRLNRRGIVYSEDLGFSPVNTDVREAFRGVVERMRASG
                     FEMIDDNPGLTSSVKTWAVTACYDAAENVRGSAYNIDYLGEVARGFIDFGEQFSAADF
                     EKAQERRAVIRQHYENMFQRHKARILITPTLGCEAFLHGSIHPHKIDDTHIQMPWLDW
                     ASFLYDANLTGMPACAIPMGIGDEGL"
BASE COUNT         1668 a         2625 c         2567 g         1675 t
ORIGIN      
        1 gcacgacctt tcccgtcagg cccatgcaca ggttcgtgcg accgtgaaaa gcgcccgaga
       61 acgctatgac tccaggtcgc ccggttttga accgcgcaat cttgatcgcg ttttccaccg
      121 cctcggcacc ggtcgaaaga aagatggtcc gcgcgtcggc gatcggcacc gcccggttca
      181 ggcgctcggc gagttccacg accggcgtat acggcgtcac catcacgcag gtgtggctga
      241 acctgttcaa ctgctcggcg accgcggcgg tgatctgcgg gtgacaatgt cccgccgaac
      301 acaccgcgat gccggtgccg aaatcgatgt agcgcttgcc ttcgacgtcc cagatttccg
      361 agcccttcgc atgctcgacg tagaccgggg cgatgttgcc ctggccgcgg gccaccgcgt
      421 cttccttgcg cgcctgccag tcactgttgg acagcccgtg aaactcgggc acttgtaatt
      481 ccgcgttact cattggtcta tgcctccgag gcacatgtat ttgatttcga gataatcgtc
      541 gagcccgtac ttcgagcctt cgcggccctg gcccgactcc ttgacaccgc cgaacggcgc
      601 catttcgttg gagatgatgc cctcgttgat gccgacgatg ccgtattcga gcgcctcggc
      661 gacccgccag atgcggccga tatcgcgcga gtaaaagtaa caggcgagcc cgaactcggt
      721 atcgttggcc atgcgaatgg cttcttcctc ggtcttgaac ttgaacagcg gtgcgatcgg
      781 cccgaagatt tcctcgttga agacgcgcat ctcgccgctg acattggtga gtatagtggg
      841 ttcgaaaaaa tgcgcgccga gatcggagcg atgcccgccg gcgaccattt tcgcgccctt
      901 ggcgagcgca tcctcgataa atccttccat ttcattggcg gcctgctcgg tgatcagcgg
      961 accgatcttg acgtcttcat cggtgcccgg gcccatcttc aggccgccga ccgcgtcggc
     1021 gagcttggcg gcgaaagcat catagacgct ttcctggacc aggatgcgat tcgcgcagac
     1081 gcaggtctgg ccggcgttgc ggtacttgca gaccagcgcg ccggcgaccg cggcgtcgag
     1141 atcggcgtcg tcaaagacga tgaacggcgc attgccgccg agttccatcg aggtgcgctt
     1201 gacggtttgc gcgcactcgg ccatcaattg cttgccgacc ggggtcgaac cggtgaaggt
     1261 cagcttgcgc acgattgggt tggaagtcag ttccttgccg atcgccgagg tttcaccggt
     1321 gaccatgttg atcacgcccg gcggaatgcc ggcgcgctcg gcgagctcgg tgaacgcgta
     1381 ggccgacagc ggggtcgcgt tggcgggctt gcagaccacg gtgcagccgg cagccagcgc
     1441 cgggccgatc ttgcgggtaa gcatcgcgtt ggggaagttc cacggcgtga tgcaggcgac
     1501 cacgcccacc ggttgcttga tgcagacgat gcgcttgtcg ttggacggct gcggaatggt
     1561 gtcgccgtaa atgcgcttgc cttcctcggc gaaccactcg atgtagttgg cgccgtaggc
     1621 gatttcgccg cgcgattcga acagcggctt gccctgctcg agggtcatga tcagcgccag
     1681 gtcttcctgg gcttccatca tcaggttgaa ccacttgcgc atcagcgccg ctctctcctt
     1741 cgcggtgcgc gagcgccagc ccggcagagc cgcctcggcg gcctcgatcg cgcgccgggt
     1801 ttccgcggtg ctgcatttgg cgactgcggc gatgacttcg ccgttcgcag ggttggtaac
     1861 cggcacggtc tcaccgctgt cggcgtcgac ccatttaccg tcgatgtagg cctgggtttt
     1921 gagcaggccc gggtcggaaa tattgatgct cattcgggat aaccctcggg ggatagtaag
     1981 caggggcgag aaggataacg atttcggctt ctggctacaa cgagtttgat ctcggctgcg
     2041 aaccttgccc ttagcgtggc gttatttgtt actatttcga cattattcaa gatccgccgc
     2101 gaataataac agcacccgga ggggggaaaa tgcccgataa cgctagcccg aataagggca
     2161 aggcacgcac taacctggat ttctatcaca gcccgacgca actgcgcttc gatacctggc
     2221 atcggataga ggagaacacc agccggttgc gcgaaaaaca catgcgcaag gccgatatcg
     2281 ccgcgctgtc gaaaaaaatc cgcgccgacc tggcgaccat cgaggttttc gaaagttatt
     2341 ccgcctttcc ctcgcgggac gacgtgaagc tcatgtggca gctcttcgac cagcaggatt
     2401 acgatcacct ggcgcggatc gtggcgcgca tcgtgcgcgc gttgaccaac ggcacctacc
     2461 gctcgacggc gatagatctg cattcgtcga tcgacgtcga tgacggcgac gaatccctgc
     2521 aaaacttcga cgacggtcac gagccgaggc cctacttcga actgctattt gtcgacgaga
     2581 gcgggcacga ggacattcgt cgcctgcgcg agcgcctgca cgagctacgc cggccggaag
     2641 ataatttcgt atacgacatc gtcgtcgtac cgagtttcga ggatgcactg atcgcggtgc
     2701 tgttcaatta caacatccag gcggtcgtaa tccgttacgg ttttccgctg cgctcggtca
     2761 atcatctcga aattctgcaa cgttacctgt cgaaaatcga cgaatcggag tacgaggacg
     2821 cgctcgatat cgagcgcggt ccgttactcg gcaacctgat ctcggaaatg cgccccgagc
     2881 tggatctgta cctggtcacc gatgccgcgg tggaggacat cgccggtaac gtgacgcaga
     2941 agttcaaccg tattttttac cagcaggaag actatctcga tctgcatttg aacgtactgc
     3001 gcggcatcca ggctcgatac gaaacgccgt tctttaccgc tctgcgcaga tacagccggc
     3061 agccgaccgg tgtcttccac gcgatgccta tctcgcgcgg caagtcggtg acgcgctcgc
     3121 actggattcg cgagatggtg cagttttacg gcatcaatat tttcctcgcc gaaacctccg
     3181 cgacttccgg cggcctcgat tcgctgctgc agccgcatgg gccgatcaag aaagcccagg
     3241 aactcgccgc gcgcgcgttc ggcgccaggc gcacttactg ggtcaccaac ggcacctcga
     3301 ccgccaacaa gatagttgta caggcgctgg tccagccggg agatatcatt ctgatcgatc
     3361 gcgactgcca taagtcgcac cactacggca tggtactgtc gggagcccat gtctgttatc
     3421 tcgattccta cccgctcgac gaatactcga tgtacggcgc ggtgccgctc aaggaaatca
     3481 agcgcaagtt gttcgaatac aagaaggccg gcaagttgca caaggtcaag atgctgctgt
     3541 tgaccaactg tactttcgat ggtgtggtct acaacgtcga gcgggtgatg gaagaatgcc
     3601 tggcgatcaa gccagacctt atctttctgt gggacgaggc ctggttcgcg ttcgcgggat
     3661 tcacgccgac ctaccgccag cgtaccgcga tgaataccgc ggcccgcctg cgaaagcgct
     3721 atgcgagcgc cgagtaccgg gccgagtacg aggtttaccg caagcaaatt tcgaatgcca
     3781 gcgaagccga cttgctgacc gaacgcctga tgccggatcc tgacctggtc cgggtgcgca
     3841 cctacgcgac gcaatcgacg cacaagtcac tgacctcgct gcgccagggc tcgatgattc
     3901 atgtctacga ccaggatttc ggtttcaagg tacaggagcc ttttgacgaa gcgttcatga
     3961 cgcacacctc gacttcgccc aattaccaga tcctcgcctc gctggacatg ggtcgccggc
     4021 aggtcgagct ggagggattc gagatggtgc agaaacaggt ggagctggcg atgacgattc
     4081 gcgaacgcgt cgcttcgcac cagttgctga gcaagtattt ccgctttcta aagcccagcg
     4141 aaatgatccc cgccgagttt cggacaacgg gtatcggcag ttacttcgat ccgaagaaag
     4201 gttggggttc cacggaaatg gaaaacgcct gggccgaaga tgaattcgtc atcgacctta
     4261 cccgtctcac gctttacctg gggctgaccg gcatcgacgg cgacaccttc aagaacaagt
     4321 tcctgatgga taagtacggc atccagatta acaagacctc gcgcaacacg gtgttgttca
     4381 tgaccaatat cggcaccacg cgttcgtcgg tcgcttttct gctcgaggtg ctggtgaaaa
     4441 tcgccaccga actcgaccag gagcgcgccg aggccagtcg cgtcgagcgc cgcctgatcg
     4501 acaaacgggt caaatcgctg tcgcaggatt tcccgccgct gcccgatttc agtcacttcc
     4561 atgccgcctt cgtgcccaag tccaaaagca agatctggac cggtgaaggc gacatacggc
     4621 gcgcgttttt tctggcctac gatgaagaaa attgcgagta catgacgatc gacgagcaga
     4681 ccatcggcaa ggcgctgaaa tcgggccgcg aactggtctc ggcgagcttc gtcacccctt
     4741 atccgccggg cttcccggtg ctggtgcccg gacaggtgat cagcgaacag atcctcgcct
     4801 tcatccgcgc gctcgatgtc aaggaaatcc acggctacga acccgagatc ggcctgcagc
     4861 tgttcaccga ggaagccctg caaaagctgc aacaatcctt accctgattc gggtgatccc
     4921 cacttatccc caatttggga tctaaatttc tgaattttgg atctgaccca aattaggggg
     4981 ttttggaaaa atggctggcg gagtcgattt tcgagaaacg gcttcgatcc cggggcgggg
     5041 attagaactg cagggcgggt atcggggcgc cggtgtagat ttcgctgtag gtctggctct
     5101 ttctcggata accctcggga tcgacgaagt cgatacgata ttcacggtcg ctaaaggtgt
     5161 cgaagccgaa accccagcta ttgtgcggcc agaagtattc gcaactgcgg tggcgcgggt
     5221 aaaaggccgc gctgtaaagc gtaccgaaac cgcgcgcgaa cttactgctg tacagcggtg
     5281 tgttgaggaa ttcgcgagag aactggaacg cactgtgctg gcgcgcctcg ccgatcgcgg
     5341 cgaggtgcgc gtagcgcaga tgggtggcga ctttttcctc gtagatcggc cagctgctgt
     5401 caggctggtg gttggtggcc agcggcgtgc ccagcgattg cggctgccgg ccgggtccga
     5461 tggcaagcgt atcgaagcgg tcgtcggcgt cgagcagggt gatgttatag gcgccgtgta
     5521 ccgggatgcg ctgtagcagt tcggtggcct cgccgacgtt gccggcgaat tccagcaggc
     5581 agcgcatcag cagcggaata ccgaagccgg tgccgaattc ctcgcggccg ccgaacgaca
     5641 gcgacaccgc cagcccgtcg gaattgatgc cgtcgagcgc cccggacatg cagtcgagca
     5701 tcgccatcac gccgcgctgc tcgaaacggc ttttcatcac cagtccgtcg aacacgtacg
     5761 gcgagtaatc gtagttgcgc accagcaccg attcgtcctg caggtaaacg gcctgcgagc
     5821 aaccgcggaa caacggcggt ggacaatact gggcgagaaa acgcgccgcg tggtcgccgc
     5881 cgccggccag gtccgtcatc gcctcgtaac aggggaggaa ctccggaata tatttcttca
     5941 gtgcctgtac ggactcgaaa taggtcggcc ggtcgcgttc gccatagcgc agaaaccagc
     6001 gccggtaacc gggccagagg cgcctgaagt gcttttgcca gagcggaccc ggccgatcct
     6061 cgccaaaggc ccgaaaggtg aacctcatgt ggatcggggg attcattcag aacggcgaga
     6121 acggcgggtc gagtaccgcc ggcgaggccg gcagcgcggt ttcctcgggt tcggtcatcg
     6181 ccgagccgac cggctcgagg tcgttacgcg tccaccagta ccagaatccc tgctcgcgca
     6241 gccagccggc gaggcgatcg atgctgttcc actcgcgccc ggctccgcgc gcgttgaata
     6301 cccggcacgg ctggctgtcg acgtagaccg ccaccgccca ggccagcgga tgcttgtcct
     6361 gatcgccgta gccgccgccc tggaacaggc gttcgacgcg gtaaaccagc gcggtttcgc
     6421 cgagcctccc ggccgtgcgc aacggcgcca gatcggcctg ggtgaccgat ccgacgattc
     6481 cgagctcaac atcttgactc atggtgttga ctaattagta aacgttaact acattatgct
     6541 tcatgatgta ggtagttaca aattttctgc ctgcattgca gcgatcttcg agggcaggtt
     6601 tgccccgcat gtattcacac cacactatgg agcatattgt gagcaaaaaa gtttccccca
     6661 ttccgcgcgg ttaccgtacc gcgacgcccg tgctgaccgt gttcgatatc gacagcgcag
     6721 tcggctttta ccaggccgcc ttcggtgccg agctgctgac ccgcaacgcc gccgccgaca
     6781 atatcgcgat caatgccagc atcaagatcg gcaacagcat cgtcatcctg aatcgcgaag
     6841 caccgcaaca gggcattttc tcgccgctga gcctgggcgg ctcgggcggc cacactcacc
     6901 tctatgttga cgacgtcaac accagctggg agcgcgccat cgaggcgggt gcccaggtac
     6961 gggtagcgct cgccgatacc tactggggcg atcgcagcgg tgtgctggtc gacggtaacg
     7021 gccacctttg gtcgatggcc agcaagatcg agaacgtctc ccaggacgaa atcgcgcgcc
     7081 gcgcgcaggc gctctgcgag cccgccgagg ccgccgcgcc ggcagcgatc tacgacttcg
     7141 cggacgccga gtacctgccc gagccgatgc cggccgagga agtgcttgca ggcgataagg
     7201 ttgctgtcta agctactgtc attcgttaga aaacccaggg gccgggaatc atcccggccc
     7261 gctcaacccg cgtctgcccg tatgccggaa attgccctga ccgccaccca gcagttaaaa
     7321 agtctcgcct caggacagat ctccgccgtc gaccttctcg aacagaccat cgcccgtgcc
     7381 gaggaactcg gcccgctgct caacccgatt gcgttgaagc tctacgcccg agcgcgccag
     7441 gcggcgctgg aagcggatcg caagcgcgcc gccggcaagg ccggccgcct gtgtggcctg
     7501 ccgatcacaa tcaaggattc gcaattcctc gccggttatc cctgcgccaa cggttcgcac
     7561 gtgctctccg atttcgtgcc cagcgaaacc tgccgcgcta tcgaattgct ggaacaggag
     7621 ggcgcggtga tcttcgccaa gaccacctgt ccggagttca gcctcagcgg cgtcacccat
     7681 tcagagattt acggcctgac gtccaacccc tggaacccgt cgcgcacctg cggcggctcg
     7741 tcgggcggcg ccgcggtagc ggtcgcatcc ggcatgggcg ccttttcgct cggtggcgac
     7801 ggcggcggtt cgattcgtat tccggccgga ttttgcggta tcgtcggctt caagccgagc
     7861 ttcggcatgg tgccgcgcga gccctgtttc ccgtcctggc aatcgatcgt tgcctacggg
     7921 ccgatgacgc gcagcgtcga ggacgcgaga ctgttcttcg acatcatcgg cgaaaatttt
     7981 accggtaacc gcaatccgcg actcaacagg cgcggtatcg tttattccga ggacctcggt
     8041 ttctcaccgg tcaacaccga cgttcgcgaa gcgttccgcg gcgttgtcga gcgcatgcgg
     8101 gcaagcggtt tcgagatgat cgatgacaac cccgggctga cctcttcggt caagacctgg
     8161 gccgtgaccg cgtgctacga cgctgcggaa aacgtgcgcg gcagcgccta caacatcgat
     8221 tacctgggcg aggtcgcgcg cggtttcatc gatttcggcg agcagttcag cgccgcggat
     8281 ttcgaaaagg cgcaagaacg gcgcgcggtt attcgtcagc actatgaaaa catgttccaa
     8341 cgccacaaag cccgcatcct gatcacgccg acgctcggct gcgaggcttt cctgcacggc
     8401 agcattcacc cgcacaagat cgacgatacg catatccaga tgccgtggct cgactgggcg
     8461 agctttctct acgacgccaa cctgaccggc atgccggcct gcgcgattcc gatgggcatc
     8521 ggcgacgagg gcctg
//
LOCUS       DATCZV010000379         7863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_454861, whole genome shotgun sequence.
ACCESSION   DATCZV010000379 DATCZV010000000
VERSION     DATCZV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454861"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VIW27_08555"
     CDS             <1..449
                     /locus_tag="VIW27_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY5739763.1"
                     /translation="SGDSSGELLCAALHDSGFATSASEPVAGEGARMHACRITNAVKC
                     VPPQNRPVAGEINACNRFLRAEIAAMPAGAVLLALGGVAHRAILKALDLRAGGFKFRH
                     AAEFALPNGMLLVDSYHPSRYNQNTGRLTAAMLARVIDRIGSLLHG"
     gene            442..2271
                     /gene="uvrC"
                     /locus_tag="VIW27_08560"
     CDS             442..2271
                     /gene="uvrC"
                     /locus_tag="VIW27_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY5739764.1"
                     /translation="MASRGEFDHKAFLATVSSKPGVYRMLDRHDRVIYVGKAKNLKKR
                     IASYFRNTGLTTRTLSLVNNIHRIEVTNTRTESEALLLENDLIKNLNPRYNVLFRDDK
                     SYPYICLSNHEFPRLKVFRGKPDRRKGSFYGPFPSAGSIRYTINHVQRMFQLRNCEDS
                     AMQNRARACLQYQIKRCTGPCVGFTDRDSYRRQIEQAKMFLDGRSSELIDQQIELMEE
                     YSARLDFEQAAQVRDRIETLRRVTEKQFVTNFDGDIDIIACEVRDDFSCIQLFMIRNG
                     TSLGNKPFFNRARLDTDAASLLESFILQHYSNHPAPPEIILSHALEDPAPLAESLERL
                     QGSRVRLTHSVRGKRRRALENAVANARQALESYLLSASMLSRRYQNLVEILHLEAPPQ
                     TIECFDISHTMGESTQASCVVFGPDGALNSEYRRYNIKDIQAGDDYAAMRQVLERRYG
                     KRVGTPEQLPDLILIDGGKGQLGVALEVLESLNIPTLKNDLRVFGVAKGAERRAGHEA
                     ILDEEMSELAISPGSPGLLLIQQIRDEAHRFAITGHRRARARSRRKSRLEEIPGIGTR
                     KRQLLLNTFGGLQGVKAAGVEELMQIKGINRETAQAIYDSFHG"
     gene            2290..2850
                     /gene="pgsA"
                     /locus_tag="VIW27_08565"
     CDS             2290..2850
                     /gene="pgsA"
                     /locus_tag="VIW27_08565"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY5739765.1"
                     /translation="MKITLPTAITLFRIALIPLFVLVFYLPVSWANIAATAIFAIASI
                     TDWVDGYLARSMQQESSFGAFLDPVADKLMVVVVIVLLVQANPSIFVALPSVVIVARE
                     ISISALREWMAQIGASATVKVSFIGKSKTLLQMVALGFMIYAEPLIGLPIFLIGLVIY
                     YLAALLTLASMVVYLRAAWPSIARHG"
     gene            2926..3001
                     /locus_tag="VIW27_08570"
     tRNA            2926..3001
                     /locus_tag="VIW27_08570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2959..2961,aa:Gly,seq:gcc)
     gene            3228..3695
                     /locus_tag="VIW27_08575"
     CDS             3228..3695
                     /locus_tag="VIW27_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEY5739766.1"
                     /translation="MRHTLSVFVLLVFIWVANSGFFTPLLLSLGLVSIVFVVWLAHRM
                     DVVDEESHPVHMTFRLPGYYCWLLIKIVQANIDVVRHVWRPKLAITPGTAVLTASQHS
                     DMGKTIYANSITLTPGTVALNVEGDQIKVHALTREGLEDLQTGEMDRRVSELE"
     gene            3700..3972
                     /locus_tag="VIW27_08580"
     CDS             3700..3972
                     /locus_tag="VIW27_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEY5739767.1"
                     /translation="MLIVVTIAILLVMLLALIRAKMGPTIYDRILAVNVFGTKTVLLI
                     TVVGFLFGRPDFLDIALVYALINFISVIGVLRYFEYTRASRERRPS"
     gene            3969..4292
                     /gene="mnhG"
                     /locus_tag="VIW27_08585"
     CDS             3969..4292
                     /gene="mnhG"
                     /locus_tag="VIW27_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012143997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEY5739768.1"
                     /translation="MIDTVTGIVSGISLGLGALLLISGSVGLLRFPDFYTRMHAAGVT
                     DTLAAGLTIVGLMLISGWNLVLVKLVLILLFIFLTGPTASHALARAAQHGGLKPMTNE
                     DKREE"
     gene            4387..5373
                     /locus_tag="VIW27_08590"
     CDS             4387..5373
                     /locus_tag="VIW27_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HEY5739769.1"
                     /translation="MSIDLLIDIALLGLLVTTALGIARTSDLFAAVMLSGIYGLLSAS
                     FFVDLDAVDVAFTEAAVGAGIAPLLMFVTLSMTGRFQRDVNILSGASLALVVSVGVLL
                     VYASFDMPAFGSPDAPAHLHVAPRYLNDSMAEIGIPNVVTSVLASYRGFDTLGEVVVI
                     FTAGVAVLGILHRPERPDDAELSATLQHHTVLRVVGKLLIPPILIFALYVQFHGDYGP
                     GGGFQAGVIFAAALILYCMLFGVQLAKRVINFALVRVLAGVGVLLYGSVGLLGILTGN
                     NYLDYDGLAADPVAGQHLGIILIEFGVGMTVAAVMLLLYLTFADRIPADGKD"
     gene            5373..5726
                     /locus_tag="VIW27_08595"
     CDS             5373..5726
                     /locus_tag="VIW27_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HEY5739770.1"
                     /translation="METIAALYNYWVVVALMTIALYIIVAQHNLVKKIIGLNIFQTSI
                     FIFYISVGNVDGGTAPILEDSFSVYSNPLPHVLILTAIVVGIATTALALALIVRIKEA
                     YGTVEEDEISARDRQ"
     gene            5723..7210
                     /locus_tag="VIW27_08600"
     CDS             5723..7210
                     /locus_tag="VIW27_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HEY5739771.1"
                     /translation="MIEHLPILQVIIPLMAAPACLVIDRPRLVWAFALAIGIATLAIS
                     VLLLQQVTSDGVISYALGGWDAPWGIEYRIDLLNAYVLLIVSAINVLALIPAYRSVKQ
                     EVGEQRQVYFYTLYLLCFAGLLGITATGDAFNVFVFLEISSLSTYALIALGKDRRALW
                     AAYQYLIIGTIGASFILIGIGLLYAQTGTLNMIDLANRIVELPNTRVVLAALVFIVVG
                     ICIKLALFPLHVWLPNAYAYAPSIVTVFVAATATKVAIYLLIRFIYSIFIGGFPESAL
                     PLQVAFIVLGVAGIFVASLVAIYQTNIKRLFAYSSVAQIGYIIVGLGLGTAIGLQASL
                     LHVFNHALMKGAIFLGLAAVMFKIGGVDLDDLAGLGRRMPWTMAAIVGGGLSLIGVPL
                     TVGFISKWYLILGAIDAGMWPLAVLLVAGSLLAVIYVWRIVETAYFRPAAEGGDQGRA
                     EAPLSFLLPLWLLLLANVYFGIDTSLTVSLSEAASNYLIGSGTGN"
     gene            7213..>7863
                     /locus_tag="VIW27_08605"
     CDS             7213..>7863
                     /locus_tag="VIW27_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5739772.1"
                     /translation="MLESWTLIAICLPFATAVGILATHRWPNLREAVSVASGVILFGI
                     IWLLLYQPILDGQRIEIHLFSVVSGIDLAFRAEPMGLLFALIASFLWFVTIIYAIGYM
                     RGHGEQNQTRFYCLFAVAIGAVMGISFAENLFTLFIFYEVLTLCTYPLVTHAGTDKAR
                     HGGRVYLGILLSTSIGFFLLGLVATWSVAGSLSFTPGGLLSEQASPLVLSAILVLFV"
BASE COUNT         1391 a         2425 c         2352 g         1695 t
ORIGIN      
        1 tcagcggcga cagttcggga gagctgctgt gtgcagccct gcatgactcg gggttcgcca
       61 cttccgcgag cgagcctgtg gccggtgagg gtgcgcgaat gcacgcctgc cggatcacca
      121 atgccgtcaa gtgcgtaccg ccgcagaacc ggccggtggc cggcgaaatc aacgcctgta
      181 atcgatttct gcgtgcggaa atcgccgcga tgcccgccgg tgcggtgctg ctggctctcg
      241 gcggtgttgc gcaccgtgca attctcaagg cgctggatct gcgtgccggc ggatttaagt
      301 ttcgccatgc cgcggaattt gcgctgccga acggcatgct gctggtcgat agctaccatc
      361 cgagtcgcta caaccagaac accggccgcc tgaccgcggc gatgctggcg cgggtgatcg
      421 accgaatcgg ttcactgctg catggctagt cgcggggaat tcgaccacaa ggcattcctg
      481 gccacggtga gcagcaagcc gggcgtctac cgcatgctcg acaggcacga ccgagtgatt
      541 tacgtcggca aggccaagaa cctgaagaaa cgcatcgcca gctactttcg caacaccggg
      601 ctgacaaccc gcaccctatc gctggtcaac aacattcacc gcatcgaggt caccaatact
      661 cgcaccgaga gcgaggccct gttgctcgag aacgacctga tcaagaatct caatccgcgc
      721 tacaatgtgc tgtttcgcga cgacaagagt tacccctaca tctgtctgag caaccatgaa
      781 ttcccgcggc tgaaggtatt tcgcggcaag cccgaccgac gcaagggaag cttttacggg
      841 ccttttccga gcgccggttc gatccgttac acgatcaacc acgtgcagcg catgttccag
      901 ttgcgcaact gcgaggattc ggcgatgcaa aatcgcgcgc gggcctgtct gcaataccag
      961 atcaagcgtt gcaccgggcc ctgcgtcgga tttaccgacc gcgacagtta ccggcgtcag
     1021 atcgagcagg cgaaaatgtt tctcgatggc cgcagcagcg agttgatcga ccagcagatc
     1081 gagctgatgg aagagtactc ggcacggctc gatttcgagc aggcggccca ggtacgcgac
     1141 cgcatcgaga cgctgcgccg ggttaccgaa aagcagttcg tgaccaattt cgacggcgac
     1201 atcgatatca tcgcctgcga agtgcgcgac gatttcagct gcattcagct gttcatgatc
     1261 cgcaacggca cctcgctcgg caacaaaccc tttttcaacc gcgcgcgtct cgataccgat
     1321 gccgccagcc tgctcgaatc gttcatcctg caacattact cgaaccaccc ggcgccgccc
     1381 gagatcattc tcagccacgc gctcgaggat ccggcaccgc tggccgagtc gctggagcgg
     1441 ctacagggtt cgcgcgtgcg tctcacccac agcgtgcgtg gcaagcgccg gcgcgcgctg
     1501 gaaaacgcgg tcgccaacgc caggcaggcg ctcgaaagct acctgctgtc cgccagcatg
     1561 cttagccggc gctaccagaa cctggtcgag attctgcacc tggaagcccc gccacagacc
     1621 atcgagtgtt tcgatatcag ccacacgatg ggcgaatcca cgcaagcctc gtgcgtggta
     1681 ttcgggcccg acggcgcgct caacagcgag taccggcgct acaacatcaa ggatatccag
     1741 gctggcgacg actacgcggc gatgcgccag gtgctcgagc ggcgttatgg caagcgcgtc
     1801 ggcacccccg agcaactgcc cgacctgatt ctgatcgacg gcggcaaagg gcagctcggc
     1861 gtggcgctcg aagtgctcga atcactcaat atcccgacgc taaaaaacga tctgcgcgta
     1921 ttcggcgtcg ccaagggcgc agaacgccgc gccgggcatg aagccatact cgacgaggaa
     1981 atgtcggaac tggcgatttc accgggctcg ccgggcctgt tgctgatcca gcagatacgc
     2041 gatgaggcgc accgcttcgc gattaccggc catcggcgcg cgcgcgccag gtcacggcgc
     2101 aagtcgcggc tcgaggagat tcccggcatc ggtactcgca agcggcagct gttgctcaat
     2161 accttcggcg gcctgcaggg cgtcaaggcc gcaggtgtcg aggaactgat gcagatcaag
     2221 ggaatcaatc gcgagaccgc tcaggctatc tatgatagct ttcatggcta gcgaaagacc
     2281 cgagtaccga tgaaaataac cctgccaacc gccattacgc tgtttcgaat cgcattgatt
     2341 ccgctgttcg tgctggtgtt ttacctgccg gtgtcctggg cgaatatcgc ggcgaccgcg
     2401 attttcgcaa tcgccagcat caccgactgg gtagacggct acctggcgcg ctcgatgcag
     2461 caggagtctt cgttcggcgc cttcctcgat ccggtcgccg acaagttgat ggtggtggtg
     2521 gtcatcgtgt tgctggtaca ggccaatccg agcatcttcg tcgcactgcc ttcggtggtg
     2581 atcgtcgccc gcgagatttc gatttcggcg ctgcgcgaat ggatggcgca aatcggtgcc
     2641 agtgccacgg tcaaggtgtc cttcatcggc aagagcaaga ccctgttgca aatggtggcc
     2701 ctcggtttca tgatctacgc cgagccgctg atcggcttac cgatattcct catcggcctg
     2761 gtgatctact acctcgccgc gctgctgacc ctggcgtcca tggtcgtcta cctgcgcgcg
     2821 gcctggccat cgatcgcccg ccatggttaa gcgttggcga aggcggcgga acagcttgcc
     2881 ggttgcttga caggtcaggg ggaatcgata gaataacgct ccttcgcggg aatagctcag
     2941 ctggtagagc acaaccttgc caaggttggg gtcgcgagtt cgaatctcgt ttcccgctcc
     3001 atttaattca ttgattataa taacatttcg gtccgatatc gcggctgagg gggagttctg
     3061 aaggtgcatt tcggcatcgc actcgcaaat ggtatcgacg cactccgcca gtacgccgcg
     3121 aggtcttcgt ccccaccggc gagtgtctgc ggaaaccgcc ggcgtcgtcg aggttccgcc
     3181 gggctgccgc ctgccgtagc ggctgtctga ccccccgggc tcctgccatg cgccacacgc
     3241 tgagcgtttt cgtcctgctg gtgttcatct gggttgcgaa ctcggggttc ttcaccccgt
     3301 tgctgctgtc actgggactg gtgtcgatcg tattcgtcgt ctggctggcg caccgcatgg
     3361 acgtcgtcga cgaagaatcg cacccggttc acatgacctt tcggctgccg ggctactatt
     3421 gttggttact gatcaagatc gtccaggcca atattgacgt cgtcagacac gtgtggcggc
     3481 cgaaactcgc aatcaccccc ggcacagcgg tgctgaccgc gagtcagcac agtgacatgg
     3541 gcaagacaat ctacgccaat tcgataaccc ttaccccggg aaccgtcgcg ctgaacgtgg
     3601 aaggtgacca gatcaaggtg catgcgttga cccgtgaggg tctcgaggac ctgcagaccg
     3661 gcgagatgga tcgacgggta agcgaactgg aatgacgcca tgctgattgt cgtaaccatt
     3721 gccatattgc ttgtcatgct gctggccctg attcgcgcca agatggggcc gacgatttac
     3781 gatcgtatcc tggcggtaaa cgtattcggt accaagaccg tgctgttgat cacggtggtc
     3841 ggcttccttt tcggccgccc ggattttctc gatatcgcac tggtttatgc gcttatcaat
     3901 ttcatcagcg tcattggtgt tctgagatac ttcgaatata cccgcgcctc ccgggagcgc
     3961 aggccgtcat gatcgatacg gtgacgggta tcgtcagcgg aatctcgctg gggctgggcg
     4021 cgctgctgtt gatctcgggt tcggtcgggc tgctcaggtt ccccgatttc tatacccgca
     4081 tgcacgccgc cggcgttacc gataccctcg cggcaggcct gaccatcgtc ggcctgatgc
     4141 tgatctctgg ctggaacctg gtgctggtca aactggtgct gatcctgctg ttcatcttct
     4201 tgaccggtcc gacggccagc catgcgctcg cccgggccgc gcagcacggc gggctcaagc
     4261 cgatgaccaa cgaagacaag cgcgaagaat gagcggccac tcaccggcgg cgccctgtcg
     4321 cgcgcgacat aatcatcacc tcgataaccc gaacaggacg cctcggcggg cgcgtggacg
     4381 gcggtcatga gtatcgatct gttaatcgac atagccttgc tcggcctgct ggtgaccacg
     4441 gcgctcggca tcgcgcgcac cagtgatctg ttcgccgcgg taatgctgtc cgggatttac
     4501 ggattgctct cggcgagctt cttcgtcgat ctcgacgcgg tcgacgtcgc cttcaccgaa
     4561 gccgcggtgg gcgccggaat cgcgccgctg ttgatgttcg tcaccctgtc gatgacgggc
     4621 cgcttccagc gcgatgtgaa tattctctcc ggtgccagcc tggcgctggt ggtttcggtc
     4681 ggcgtgttac tggtctacgc gagtttcgac atgccggcat tcggctcgcc cgatgcgccc
     4741 gcgcacctgc acgtcgcgcc gcgctacctg aacgattcga tggcggaaat cggcatcccc
     4801 aacgtcgtca cctcggtgct ggccagctac cggggcttcg ataccctggg cgaggtagtg
     4861 gtcatattta ccgccggcgt cgccgtgctc ggcattctgc atcgtcccga gcggccggac
     4921 gacgcagaat tgtcggcgac gctgcaacac catacggtgc tgcgggtcgt cggcaagctg
     4981 ctgatacccc cgatcctgat ttttgctctc tatgtgcagt ttcacggcga ttacgggccc
     5041 gggggagggt tccaggccgg tgtcattttc gccgcggcgc tgattctcta ttgcatgctg
     5101 ttcggcgtac aactggcaaa gagggtgatc aattttgcgc tggtacgggt gctggccggt
     5161 gtcggcgtgc tgctttacgg cagcgtgggc ttgctcggaa tattgaccgg caacaactac
     5221 ctcgattacg acgggctcgc ggccgacccg gtggcaggcc agcacctggg tatcattctg
     5281 atcgaattcg gtgtcggcat gacggtggcg gcggtgatgc tgttgctgta cctgaccttt
     5341 gccgatcgga tcccggccga cgggaaagat tgatggaaac gattgccgcg ctttacaact
     5401 actgggtggt cgttgcgttg atgacgatcg cgctgtatat catcgtcgcg cagcacaatc
     5461 tggtcaaaaa gattatcggc ctgaatattt tccagacgtc gattttcatt ttctacatca
     5521 gcgtcggtaa cgtcgacggc ggcacggcgc cgatactgga agactcgttc agcgtttatt
     5581 ccaacccgct gccgcacgtg ctaatcctca ccgcgattgt cgtcggcatc gcgaccacgg
     5641 cactggcact ggcgctgatc gtacgtatca aggaagccta cggcacggtc gaggaagacg
     5701 aaatttccgc acgggaccgg cagtgatcga gcatctgccg atactgcagg taatcattcc
     5761 gctgatggcg gcgcctgcct gcctggtcat cgaccgcccg cggctggtct gggcattcgc
     5821 cctcgcgatc ggcatcgcca cgctcgcgat cagcgtgctg ctgttgcaac aagtgacttc
     5881 cgacggcgtc atcagttacg ccctcggcgg ctgggatgca ccctggggta tcgagtaccg
     5941 tatcgacctg ctgaacgctt acgtactgtt gatcgtctcg gcgatcaacg tgctggcctt
     6001 gattccggcc taccgcagcg tcaaacagga agtcggcgag cagcgacagg tctattttta
     6061 caccctctac ctgttgtgct tcgccgggct gctggggatt accgcgaccg gcgacgcctt
     6121 caacgtattc gtattcctcg aaatatcgtc gctgtccact tatgcgctga ttgccctcgg
     6181 taaggatcgc cgggcattat gggccgccta ccagtacctg atcatcggca ccatcggggc
     6241 atcgttcatc ctcatcggta tcggcctgtt gtatgcgcag accgggaccc tgaacatgat
     6301 cgatctggcg aaccgcatcg tcgaactgcc aaatacccgt gtcgtgctgg cggcgctggt
     6361 gttcatcgtc gtcggtatct gcatcaagct ggccctgttt ccgctgcacg tctggctgcc
     6421 caatgcctac gcctacgcgc cgtcgatcgt caccgtgttc gtcgccgcga ccgcgaccaa
     6481 ggtggccatc tacctgttga tccgcttcat ctactcgatt ttcatcggcg gttttccgga
     6541 atcggcattg ccgttgcagg tcgccttcat cgtgctcggc gtcgcgggaa tcttcgtcgc
     6601 ctctctggtc gccatctacc agaccaatat caagcgcctg ttcgcctatt caagcgtcgc
     6661 ccagatcggc tacatcatcg tcggtctcgg ccttggcacc gcgatcggcc tgcaggcgtc
     6721 gctgttgcac gtcttcaatc atgcgctgat gaagggcgcg atcttcctcg gcctggccgc
     6781 ggtcatgttc aagatcggtg gcgtcgacct cgacgacctg gcgggcctcg gccggcgcat
     6841 gccgtggacc atggcggcga tcgtcggtgg cggcctgagc ctgatcggcg tgccgctaac
     6901 ggtcggtttc atcagcaagt ggtacctgat actcggcgcc atcgatgccg gcatgtggcc
     6961 gctggcggta ctgctggtcg ccggttcgct gctggccgtg atctatgtct ggagaatcgt
     7021 cgaaacggcc tatttcagac cggcggctga aggcggcgac cagggcaggg ccgaagcgcc
     7081 gctcagtttc ctgctgccgc tgtggctgct gctgctggca aacgtctatt tcggtatcga
     7141 caccagcctg accgtttcgc tgagcgaagc cgcgtcgaat tacctgatcg gcagcgggac
     7201 gggtaactga agatgctcga atcctggacc ctgatcgcga tttgcctgcc gtttgccacc
     7261 gccgtcggca tcctcgccac gcatcgctgg ccgaatcttc gcgaagccgt ttcggtagcc
     7321 tccggggtaa tcctgttcgg catcatctgg ctgctgctct accaaccgat tctcgacggg
     7381 caacggatcg aaattcacct tttcagcgtg gtttccggta tcgatctggc gtttcgtgcc
     7441 gaacccatgg gactgctgtt cgcgctgatc gcgagcttcc tgtggttcgt caccatcatc
     7501 tacgcgatcg gctacatgcg tggccacggc gaacaaaacc agacccgctt ttattgcctg
     7561 ttcgcggtcg ccatcggcgc ggtgatgggc atttcgttcg ccgagaacct gtttacgctg
     7621 ttcatctttt acgaggtcct gaccctgtgc acttacccgc tggtcactca cgcgggcacc
     7681 gacaaggccc gccacggcgg gcgcgtatac ctgggcatcc tgctgtcgac ctcgatcggc
     7741 tttttcctgc tggggctggt ggcgacctgg tcggtggccg gcagcctgtc ctttaccccg
     7801 ggcggcctgc tgtcggagca ggcttcgccg ctggtattgt cggccattct ggtactgttc
     7861 gta
//
LOCUS       DATCZV010000380         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_349329, whole genome shotgun sequence.
ACCESSION   DATCZV010000380 DATCZV010000000
VERSION     DATCZV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349329"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..435
                     /locus_tag="VIW27_08610"
                     /pseudo
     CDS             1..435
                     /locus_tag="VIW27_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008554937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
     gene            642..1802
                     /locus_tag="VIW27_08615"
     CDS             642..1802
                     /locus_tag="VIW27_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5739773.1"
                     /translation="MHFGLSSEQQMIVDTVREFVEKEIYPHETEVERTGEVPLELGHE
                     IRDKCLEAGYFAANIAEEFGGGGLGHVDFTLLERELGRASNALAVFFGRPSGILQACN
                     AEQREKYLLPAVRGEKFDALAMTEPGAGSDVRGMQCNAKPDGDDWIVNGSKHFISHAN
                     IADFVIVFVATGEEDTPRGPKKKITCFLVDRGEPGFEIRPGYNSVSHRGYQNCILNFD
                     NCRLPSSQILGELHGGFEVMNEWLYATRLTVAATSVGRARRAFEYALPYCAERKQFGQ
                     QIGKFQGVSFKLADMITEIDAADWLTLAAAWRLDEGLDANREIASAKVYATEMLARVT
                     DEAIQIFGGMGLMDELPLERFWRDARVERIWDGTSEIQRHIISRDLLRPLGG"
     gene            1817..3868
                     /locus_tag="VIW27_08620"
     CDS             1817..3868
                     /locus_tag="VIW27_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5739774.1"
                     /translation="MRLERMLRPRSIAVIGGRWAANVVEQCKLMGFAGDIWPIHPHRE
                     DVHGIRAYRSVDDLPGSPDAAFIGVNRELTVDVVRGLRARDCGGAVCFASGWRESGED
                     GDALQARLLDAAGEMPVLGPNCYGYINYADGALLWPDQQGGKRLGDNENGAAIIAQSS
                     NIAINFTMQKRGLPLSYVFTVGNQAMVGISELASNLLEDARVSTLGIYLEGFDSVAGF
                     EALARKSRALRKPVVIFSVGKSAKAQASALSHTASLVGDRAIVGEFLRRCGFGQADSI
                     PVFLEALKLLHLHGPLDGYRLSSMSCSGGEASIIADAAHGRRVYFPDLDAPQKKALQD
                     ALGPRVAIANPLDYQTYCWGDRQTMQAAYSAMAAIGFDLNLLVIDFPHPARCSDADWH
                     IAVDAFDAAIRDNDARGAFVVGMAENIREEYTEDYRGRGLVAFYGIEEAIRACEIAAD
                     IGSAWQREPPAPLHLPLVFTGTRKTLDEAAAKQALAAHGVPLPAGGIVESVAAAQALA
                     ADIGYPVVLKALGIAHKTEQNAVRLNLASADDVEQAAGELLTLAPRLYLEKMHASVAE
                     LIVGVVRDAQFGPVLTLGSGGILVELLQDVVSLLLPASREDIVRALGELRSAPFLEGF
                     RGRPRADSEAAIDAILAVQQYALARAEALVELDVNPLLVGAEGQGAFAADALIVIEEQ
                     Q"
     gene            3868..>4246
                     /locus_tag="VIW27_08625"
     CDS             3868..>4246
                     /locus_tag="VIW27_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5739775.1"
                     /translation="MSDVINTRREGAILEVTLDRPKANAIDLATSRLMGETFQAFRDD
                     PELRVAIVRTAGDKFFSAGWDLKAAADGDAVDGDYGVGGFAGLQELRGLNKPVIAAVQ
                     GMAVGGGFELALSADLIYASDTSS"
BASE COUNT          774 a         1378 c         1374 g          720 t
ORIGIN      
        1 cactcgatcg gcatgcaccc gctacatgtc aaaaaggaaa tcgcagcctt catcgccgat
       61 cggctactcg aagccatctg gcgcgaatcg ctgtggctgg tcaaggacgg cattgcgact
      121 accagggata tcgacgacgc cgtacgttac ggcttcggcc tgcgctacgc gcaaatgggc
      181 gtattcgaca cctatcgcgt cgccggcggc gaggccggta tgcgccactt catggaacag
      241 ttcggcccct gcctgcaatg gccctggacc aagttgacgg acgtgcccga gttcaccgac
      301 gaactggtcg acctgatcgc cgggcagtcg gacgagcagt ccggatacat gagcattcgc
      361 gaaatggaac gcatccgcga cgacaacctg atcgcgatcc tgcaggcgtt gaagggcaac
      421 gactggggcg caggcagaac gctggcgcgg tacgaggcgc acctgcgcga ggccgccaac
      481 gacggctgac gggcgccaaa ctcgacaaga agtcattccc gggcacgcga aataaaatgg
      541 ggtcagagtc atttaatttt cgccgccgcg taccgggctg cgcgagaaaa tcgaaaaaat
      601 taaatgactc tgaccccatt ttttaacgga gagatttgat gctgcatttt ggcttgagct
      661 ccgaacagca aatgatcgtc gatacggtcc gcgagttcgt cgagaaggaa atctacccgc
      721 acgagaccga agtcgagcgc actggagaag tgccgctgga gctcggccac gagattcgcg
      781 acaagtgcct cgaggccggc tacttcgccg ccaacattgc cgaggaattc ggcggcggcg
      841 gcctcggcca cgtcgacttc acgctgctgg agcgcgaact cgggcgcgcc tcgaacgcgc
      901 tcgcggtgtt cttcggccgc ccgtccggca tcctgcaggc ctgcaacgcc gagcagcgcg
      961 agaaatacct gttgccggcg gtgcgcggcg aaaagttcga cgcgctggcg atgaccgaac
     1021 ccggcgccgg ctccgacgtg cgcggcatgc agtgcaacgc caaacccgac ggcgacgact
     1081 ggatcgtcaa cggcagcaag catttcatca gccacgccaa tatcgccgac ttcgtcatcg
     1141 tcttcgtcgc caccggcgag gaagataccc cgcgcggacc gaagaaaaag atcacctgct
     1201 tcctggtcga ccgcggggaa ccgggtttcg aaattcgccc gggttacaac tcggtcagcc
     1261 atcgcggcta ccagaactgc attctcaact tcgacaactg ccggctgccg tcctcgcaaa
     1321 tcctcggtga actgcacggc ggtttcgagg tcatgaacga atggctctac gcgacccggc
     1381 tgacggtcgc cgcgaccagt gtcggccgcg cgcggcgcgc gttcgaatac gcgctgccgt
     1441 actgtgccga gcgcaagcag ttcggccagc agatcggcaa gttccagggc gtatcgttca
     1501 agctcgccga catgatcacc gagatcgacg ccgccgactg gctgacgctg gccgccgcct
     1561 ggcgcctcga cgaaggcctt gacgccaacc gcgaaatcgc cagcgccaag gtctatgcca
     1621 ccgaaatgct ggcgcgggtc accgacgagg cgatccagat cttcggcggc atggggctga
     1681 tggacgaact gccactggag cgcttctggc gcgacgcgcg ggtcgagcgt atctgggatg
     1741 gcacctcgga aatccagcgg cacatcattt cgcgtgacct gctgcgcccg ctgggtggct
     1801 gacggagatc ctgccgatgc gcctcgaacg catgctgcgc ccgcggtcga tcgcggtgat
     1861 cggcggccgc tgggccgcca acgtggtcga gcaatgcaag ctgatgggtt tcgccggcga
     1921 tatctggccg atccatccgc accgggaaga cgtgcacggc attcgcgctt accgctcggt
     1981 cgacgacctg cccggcagtc cggacgcggc gttcatcggc gtcaaccgcg aactgaccgt
     2041 cgacgtggtg cgcggcttgc gcgcgcgcga ttgcggcggc gcggtctgtt tcgcatccgg
     2101 ctggcgtgag agcggcgagg acggcgacgc gctacaagcg cgattgctcg atgcggccgg
     2161 cgaaatgccg gtgctgggac cgaattgcta cggctatatc aactacgccg acggcgcgct
     2221 gctgtggccc gaccagcagg gtggcaagcg tctcggtgac aatgaaaacg gcgccgcgat
     2281 catcgcccag tcgtccaaca tcgcgatcaa cttcacgatg caaaaacgcg gcctgccgct
     2341 gtcctacgta ttcacggtcg gcaaccaggc gatggtcggc atttccgaac tggcgtcgaa
     2401 tctgctcgaa gacgcgcgcg tgtccacgct cggcatctac ctcgaaggct tcgattcggt
     2461 cgccggcttc gaggcactgg cacgcaaatc gcgcgcgctg cgcaagccgg tagtcatctt
     2521 cagcgtcggc aagagcgcca aggcgcaggc ttccgcgctg tcgcacaccg cgtcgctggt
     2581 cggcgaccgc gcgatagtcg gcgaattcct gcgccgctgc ggcttcggcc aggcggattc
     2641 gatcccggtg tttctcgaag ccctgaagct gctgcacctg cacggcccgc tcgacggtta
     2701 tcggctgtcg tcgatgagct gttccggcgg cgaggccagc atcatcgccg atgctgccca
     2761 cgggcgccgg gtctattttc ccgatctcga tgcaccgcaa aagaaggctt tgcaggacgc
     2821 actcgggccg cgggtcgcga tcgccaatcc gctcgattat caaacctatt gctggggcga
     2881 ccggcagacc atgcaggccg cctacagcgc gatggccgcg atcggtttcg atctcaacct
     2941 gctggtcatc gattttccgc acccggcgcg ctgcagcgat gccgactggc atatcgcggt
     3001 cgacgccttc gatgccgcga tccgcgataa cgacgccagg ggcgctttcg tcgtcggcat
     3061 ggcggagaat attcgcgagg aatacaccga agactatcgc ggccgcgggc tggttgcttt
     3121 ttacggtatc gaggaagcga ttcgcgcctg cgaaatcgcc gccgatatcg gcagcgcctg
     3181 gcagcgcgag ccgcccgcgc cgctgcactt gccgctcgtc ttcaccggca cgcgcaaaac
     3241 gctcgacgag gccgccgcca agcaggcgct ggccgcacac ggcgtgccgc tgccggcagg
     3301 cggcatcgtc gaatcggtgg ccgcggcgca ggcgctcgcc gccgacatcg gttacccggt
     3361 ggtgctgaaa gccctcggta tcgcgcacaa gaccgagcag aatgccgtgc gcctgaatct
     3421 cgcctccgcg gacgacgtcg agcaggccgc cggcgagctg ttgacgctag ccccgaggct
     3481 gtatctggaa aaaatgcacg cttcggttgc cgagctgatc gtcggcgtgg tgcgcgatgc
     3541 gcagttcggc ccggtgctga cgctcggcag cggcggcatc ctggtcgagt tattgcagga
     3601 cgtcgtttcg ctgctgctgc cggcgagccg cgaagacatc gtgcgcgccc tcggcgagct
     3661 caggtcggcg ccgtttttag agggttttcg cggtcggccg cgcgccgaca gtgaagccgc
     3721 tatcgacgcg atcctcgcgg tgcagcagta tgcgctggcg cgagccgagg ctctcgtgga
     3781 actcgatgtc aatccattac tggtcggtgc cgagggccag ggcgcgttcg cggccgacgc
     3841 actaatcgtc atagaggagc agcaataatg tccgatgtca tcaacacccg tcgcgaaggc
     3901 gcgatactcg aggtcacgct ggatcgcccc aaggcgaacg ccatcgacct cgccaccagc
     3961 cggctgatgg gcgaaacctt ccaggcattt cgcgacgacc cggaactgcg cgtggcgatc
     4021 gtgcgcaccg ccggcgacaa gttcttcagc gccggctggg acctcaaggc cgccgccgac
     4081 ggcgacgcgg tcgatggcga ctatggcgtc ggcggctttg ccggactgca ggaactgcgc
     4141 ggcctcaaca aaccggtgat cgccgcggtc cagggcatgg cggtcggcgg cggcttcgag
     4201 ctggcgctgt cggccgacct gatttacgcg tcggacactt cgagct
//
LOCUS       DATCZV010000381         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_401775, whole genome shotgun sequence.
ACCESSION   DATCZV010000381 DATCZV010000000
VERSION     DATCZV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401775"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..737)
                     /locus_tag="VIW27_08630"
     CDS             complement(150..737)
                     /locus_tag="VIW27_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY5739776.1"
                     /translation="MIMTQINLLPWREELRQEQKKEFGMTAAMSVVLAAAIVGLIHFQ
                     MQAKIDYQLSRNKFLSDEIAKLDEEIKEIAELQKVRRSLIERMEVIQDLQASRPSIVH
                     LFTEIVSTVPNGVYLETLAQTGSNLLVTGEAESNARVSTYMRNLAASDWLKEPNLTVI
                     EVEDKTVNRISSFTLTVKQTSPNATDEEEQDGGMQ"
     gene            complement(734..1807)
                     /locus_tag="VIW27_08635"
     CDS             complement(734..1807)
                     /locus_tag="VIW27_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY5739777.1"
                     /translation="MPVFLTRKKPPLFGLDISSTSVKLMELSKTGSNYRIEGIGVEPL
                     PANAVVEKNVAEVEAVGESVKRAVAKSKVKSKYCAIAVSGSSVITKKITMPANISEDE
                     MEGQIQLEADQYIPYPLEEVNLDFHVIGESENNPETVDVLLAACRSENVDDRVAAVEL
                     AGLTPKIVDIEAYTVETVFSAMAPQMPDEGMDKTVAIVDIGATMTSLSVIHSHHLIYT
                     REQNFGGKQLTEEIMRRYGLSYEEAGLAKRQGGLPDNYGPEVLDPFKETTAQQVSRFL
                     QFFYSAGGPIGDIDHLVLAGGTASLPGLAELTESHIGVPTTIANPFANMSATSRVNKA
                     NLQADAPSFLIAAGLAIRGVEYA"
     gene            2031..4814
                     /locus_tag="VIW27_08640"
     CDS             2031..4814
                     /locus_tag="VIW27_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY5739778.1"
                     /translation="MAIAVALYIYAVMVPALPSIDHLEDAQYQVPLRVYDRNEALLAE
                     FGEHRRIPVNFDKIPRYLIDAVVAVEDDQFWNHWGVDFSALLAAVYEVATTGRKTRGG
                     STITMQVARNFFLSPEQTYTRKFNEILLALKIESELSKEKIMELYLNKIFLGHRAYGI
                     SAASQVYYDKPLNELTLAQAAMIAGLPKAPSKYNPISNPERALIRRNYILGRMRILEF
                     IDQQELEVALDEPITAELHRTRVTAEAQYVSELVRAQLFEHYGDAIYKAGLRVYTSID
                     TELQAAANRALRRSLLNYTRRHGYRGVIDKIELGDLSGLAELLEDGESIGETLDSAAA
                     VDLAEAGDPDEPSGLAADPFENDLIVDNQVGGLRKGVVIAIDEGKAESAEGAGDGIPA
                     TATLLISNLERVRVPFEGGIDWAAPFIDEDNQGPKPEKVGDVLAVGDVVWLEQRDSVW
                     LLAEVPQVEGALVSINPATGGITAMVGGFDYFKNKFNRATQARRQPGSNFKPFIYSAA
                     LEKGFTAASIINDAPVVFDDDSLEATWRPENYSGKFYGPTRMREALVKSRNLVSIRIL
                     QAIGLNYASNYVQRFGFERDSMPYDLSLALGSGTFSPLQMARAFAVFSNGGYLIDPYL
                     IERVQAGNGDLLYRHQPLTVCQGCEDEDLAALEAAEAEAALQEDAAQQSAKIADTESE
                     EIPANEQATAEQSAEQPLDEAQAATDGEPDPLFDRETVKLEPVDLGPLEGIGGPAPDD
                     TVVSSEYAPRVITAQNAYIMRSMMREVVQRGTAVRAKALGRNDLAGKTGTTNDQVDAW
                     FSGFNDQVVTTSWVGFDNQRRMGRRETGGRAALPMWIDFMKSALEGMPESLQEEPRGL
                     VTVRIDAETGDRAGVETRNSLFEVFRVENAPREMAVRSTVNDTSGPVVDEGGGGTVTV
                     LPQTEQEIVEDLF"
BASE COUNT          938 a         1437 c         1562 g         1009 t
ORIGIN      
        1 cagcacgttg agctgttttt gcgtgtcgaa gtagatgccg gcggcgatga caagaacgaa
       61 cagcacggta taaacaccga tcttgatcga cgtcggcgcg gtgccgatat tctcgggttc
      121 caggtgatgc tgaatttctt cccaattcat tactgcatac ctccgtcctg ttcctcttca
      181 tccgtggcgt tgggcgaggt ttgcttgacg gtcagcgtga agctgctgat gcgattgacg
      241 gtcttgtcct cgacctcgat gacggtcagg ttcggctcct tcagccagtc cgaggcggcg
      301 aggttgcgca tgtaggtcga aacccgcgca ttggactcgg cttcgccggt gaccagcagg
      361 ttactgccgg tttgcgccag ggtttccagg taaacgccgt tgggcacggt ggagacgatt
      421 tcggtaaaca ggtgcacgat cgacgggcgg ctggcctgca ggtcctggat gacttccatg
      481 cgttcgatca ggctgcggcg gactttttgc agttcggcga tttccttgat ttcctcgtcg
      541 agcttggcga tttcgtcgga caggaacttg ttgcgcgaca actggtaatc gatcttggcc
      601 tgcatctgga aatgaatcag tccgacgatg gccgctgcca gcaccaccga catcgcggcg
      661 gtcatgccga attctttctt ctgctcctgg cgcagttcct cgcgccacgg cagcaggttg
      721 atctgggtca tgatcatgcg tactcgactc ctcgaatggc tagcccggcg gcgatcagga
      781 acgagggtgc gtcagcctgc aggttggcct tgttgacgcg gctggtcgcc gacatattgg
      841 cgaacgggtt ggcaatcgtg gtcggcacgc cgatatgcga ttcggtcaat tccgcgagac
      901 cgggcaggct ggcggtaccg ccggccagta ccaggtgatc gatgtcgccg atcgggccgc
      961 cggcggaata gaagaactgc aggaagcggc tcacctgttg cgcggtggtc tccttgaacg
     1021 ggtcgagtac ctcgggtccg tagttgtcgg gcagtccgcc ctgacgcttg gcgaggccgg
     1081 cttcctcgta ggacaggccg tagcggcgca tgatttcctc ggtcaattgc ttgccgccga
     1141 agttttgctc gcgggtatag atcaggtgat gactgtgaat cacgctgagg ctggtcatgg
     1201 tagcgccgat atcgacgatg gcgacggtct tgtccatgcc ctcgtcgggc atttgcgggg
     1261 ccatcgcgga aaacaccgtt tccacggtat aggcttcgat gtcgacgatc ttcggcgtca
     1321 gtccggcgag ttccaccgcg gcgacgcggt cgtcgacatt ctccgagcgg caggcggcga
     1381 gcagcacgtc gacggtctcg ggattgtttt cgctttcccc gatgacgtga aagtcgagat
     1441 tgacttcctc cagcgggtag ggaatgtact gatcggcctc gagctggatc tggccttcca
     1501 tctcgtcctc ggagatgttg gccggcatgg tgattttctt ggtgatcacc gacgatccgg
     1561 acacggcgat cgcgcagtac ttcgatttta ccttggactt ggcgaccgca cgctttacac
     1621 tctcgcctac cgcttcgacc tcggctacgt ttttctcgac gacagcgttg gcgggcaggg
     1681 gttcgacacc gatgccctcg atccggtagt tggagcccgt tttgctgagt tccattaact
     1741 tgaccgatgt cgagctgata tctagcccaa aaagcggcgg ttttttgcgc gttagaaaca
     1801 ccggtatgcc tcgtaaatgc agccttgatg cgagatctta gtataacaat tcacattaag
     1861 atctagttat aagtgtataa aatgttaaat tattctaatt agttcagaat gagtctagtg
     1921 caagaattcg atattgtcga tacttttcaa gtagtcgttc atgtatatag cctaacttaa
     1981 tgaaaaagct agtaaaaatt gcatcctggc tgtttgcgac aggctcgctc gtggcgatcg
     2041 ccgtggcgct gtatatttac gccgttatgg tgcctgcgct gccctcgatc gatcatctcg
     2101 aggatgccca gtaccaggtg ccgctgcgcg tctacgatcg taatgaagcc ctgctcgccg
     2161 agttcggtga acaccgtcga attccggtca atttcgacaa gattccgcgc tacctgatcg
     2221 acgccgtggt agccgtcgag gacgaccagt tctggaatca ctggggggtc gatttcagcg
     2281 cgctgctggc ggcggtctac gaggtcgcga ccaccggccg caagacgcgc ggcggcagta
     2341 cgatcaccat gcaggtggcg cgcaactttt ttctcagccc ggagcaaacc tacacccgta
     2401 aattcaacga aatcctgctg gcgctgaaga tcgagagtga gctcagcaag gaaaaaatca
     2461 tggagcttta cctcaacaag atctttctcg gccatcgtgc ctacgggatc agcgccgcgt
     2521 cgcaggtcta ctacgacaag cctctgaacg agttgacgct ggcgcaggcg gcgatgatcg
     2581 ccgggctgcc gaaagcgccc tccaagtaca atccgatttc caatccggaa agggcgctga
     2641 tccgtcgcaa ctatattctc ggccgcatgc ggattctgga gttcatcgac cagcaggaac
     2701 tcgaagtggc gctcgacgag ccgattaccg cggagttgca tcgaacccgt gtcaccgccg
     2761 aggcgcagta cgtctccgag ctggtacgcg cgcaattgtt cgagcattac ggcgacgcca
     2821 tatataaggc cggtcttcgg gtctacacca gtatcgatac cgagctgcag gcggccgcca
     2881 accgggcgct gcgccgcagc ctgctgaatt atacccggcg gcacggctat cgcggcgtga
     2941 tcgacaagat tgaactcggc gatctcagcg ggctggccga gctcctcgag gacggcgagt
     3001 cgatcggtga gacgctcgat tcggccgcgg ccgtcgatct ggccgaggcc ggcgatcccg
     3061 acgagccgtc cgggctggct gccgatccgt tcgagaacga cctgatcgtc gacaaccagg
     3121 tgggcggcct gcgcaaaggt gtggtgattg cgatcgacga gggcaaggcg gaatccgcgg
     3181 aaggcgccgg tgacggcatc cccgccactg caaccctgct gatttcgaac ctggaacggg
     3241 tgcgggtacc gttcgagggg ggcatcgact gggccgcgcc gttcatcgac gaggataacc
     3301 aggggcccaa gccggaaaag gtcggtgacg tgctcgcggt cggcgacgtg gtctggctgg
     3361 agcaacgcga ttccgtctgg ctgctcgccg aagtgccgca ggtcgagggt gcgctggtgt
     3421 cgattaatcc cgccaccggc gggatcaccg cgatggtcgg cggcttcgac tatttcaaga
     3481 acaagttcaa ccgcgccacc caggcgcgac ggcagccggg ttcgaatttc aagccgttta
     3541 tctactcggc cgcactggaa aagggattca cggcggcatc catcatcaac gacgcgccgg
     3601 tggtgttcga cgacgattcg ctggaggcga cctggcgccc ggaaaattat tcgggcaagt
     3661 tttacggccc gacgcgcatg cgcgaggcgc tggtcaaatc acgtaacctg gtgtcgatcc
     3721 gcattctgca ggcgatcggc ctgaactatg cgagcaacta cgtgcagcgt ttcggtttcg
     3781 agcgcgacag catgccgtac gacctgtcgc tggcgctcgg cagtggtacc ttctcaccgc
     3841 tgcagatggc gcgcgccttc gcggtgtttt ccaatggcgg ttatctgatc gatccgtacc
     3901 tgatcgagcg ggtccaggcc ggtaacggcg acctgctcta ccggcatcag ccgttgacgg
     3961 tttgccaggg ctgcgaggac gaggatctcg ctgcgctcga ggccgccgag gccgaggctg
     4021 ccctgcagga ggacgcggcg cagcagtcgg cgaagatcgc cgataccgag tccgaggaaa
     4081 ttccggccaa cgagcaggcc accgccgagc agtccgccga gcagcccctc gacgaggcgc
     4141 aagcggcgac cgacggtgaa cccgacccgc tgttcgatcg cgaaaccgtg aaactcgagc
     4201 cggtcgatct cggtccgctg gaagggatcg gtgggcccgc gcccgacgat acggtagtct
     4261 cgtcggagta cgcccccagg gtgattaccg cgcagaatgc ctacatcatg cgttcgatga
     4321 tgcgcgaggt ggtgcagcgc ggcaccgcgg tgcgcgccaa ggcactgggc cgtaacgacc
     4381 tcgccggcaa gaccggtacc accaacgacc aggtcgacgc ctggttcagc ggcttcaacg
     4441 accaggtcgt gaccaccagt tgggtcggtt tcgacaacca gcggcgcatg ggtcggcggg
     4501 agaccggcgg ccgcgcggca ttaccgatgt ggatcgattt catgaagagc gcgctcgagg
     4561 gcatgcccga aagcctgcag gaagaaccgc gcggactggt tacggtacgc atcgacgccg
     4621 agaccggcga ccgtgccggt gtcgaaaccc gcaacagcct gttcgaggtc tttcgcgtgg
     4681 aaaacgcgcc ccgcgaaatg gccgtcaggt cgaccgtcaa cgacaccagc gggccggttg
     4741 tcgacgaggg cggcggcgga acggttaccg tgttgccgca gaccgagcag gaaatcgtgg
     4801 aggatctgtt ctagcccagc atcgggctac ggatctcgat gtctcattca gcgccccgac
     4861 gccgtccctg cgcaggccgc aatccggcga cgacgacgcg gtgcaggctt aggccgaagt
     4921 ctggcggatc tggtagccga actcgt
//
LOCUS       DATCZV010000382         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_295740, whole genome shotgun sequence.
ACCESSION   DATCZV010000382 DATCZV010000000
VERSION     DATCZV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295740"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VIW27_08645"
     CDS             <1..766
                     /locus_tag="VIW27_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY5739779.1"
                     /translation="DKGPIIEYMNSNITMFKWMISEGYGDVRTITRRIEEMEKWLADP
                     VLLEADADAEYAEIVEIDLAEITEPLLACPNDPDDVKPLSAVTGEKIDEVFIGSCMTN
                     IGHFRAAGKLLDKLDTPVPTRLWIAPPTKMDEAQLTAEGYYGIFGRTGARTEMPGCSL
                     CMGNQARVAENATVVSTSTRNFPNRLGKGADVYLASAELAAVAAILGRIPTMGEYLQY
                     AADIDTLADDVYRYLNFNEMENYTAAADKAFPVKVA"
     gene            817..1770
                     /locus_tag="VIW27_08650"
     CDS             817..1770
                     /locus_tag="VIW27_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739780.1"
                     /translation="MKRPPRRLGSLLAFCCLLSAGIPPAAADFVGDLIRDFADSEIVF
                     TRSQSNVPFVPLAYADLSLYSDTRVRRPDDSSLEFDLATVSQGALLPLLLSPRDALLV
                     GEWAAVSRFEASSADADSFNALSAGLPVGWLRQIDDHRQFGAFVMPLAHRADLVNSSW
                     SYEVLGGAFGRHERDQRLWWVYGFYFDVGPGDDIYLPYLGASWEFDDELTLSAILPWP
                     AILYAPDRDTLWRFGAAPSGASWSLDSEQNEISFSLDNWQLGLTAEHRVSGNLWFALE
                     AGVAGLRSLRLEDGKWRSAKFDVDESAYLRVAFNFRPEPPR"
     gene            1967..>2445
                     /locus_tag="VIW27_08655"
     CDS             1967..>2445
                     /locus_tag="VIW27_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HEY5739781.1"
                     /translation="MTRSRLAEVDFSQLGFGSVFADHMFSQHYRDGSWQQPQIVPYQA
                     LALEPGVAMLHYGQTVFDGYKAFRCVDGEARIFRPEMNAKRLYQSCQRLCIPVMPLDE
                     LQQIMIEATRKLIEIDHAWIPREWGHSLYVRPLIIATEATLEVRAANSYHFMIMTSPV
                     "
BASE COUNT          425 a          778 c          771 g          471 t
ORIGIN      
        1 cgacaaggga ccgatcatcg agtacatgaa ttccaatatc accatgttca agtggatgat
       61 cagcgagggt tacggcgacg tgcgcacgat tacgcggcgc atcgaggaaa tggaaaaatg
      121 gctcgctgac ccggtgctgc tggaagccga tgccgatgcc gaatacgccg agatcgtcga
      181 gatcgacctc gccgagatca ccgagccgct gctcgcctgc cccaacgatc ccgacgacgt
      241 caagcctttg tccgcggtca ccggggagaa aatcgacgaa gtcttcatcg gttcctgcat
      301 gaccaacatc ggccacttcc gcgccgccgg caagctgctc gacaagctcg acacgccggt
      361 gccgacgcgc ctgtggatcg cgccgccgac caagatggac gaggcgcagc tgaccgccga
      421 gggttattac ggcatattcg gtcgcaccgg tgcgcgcacc gagatgccgg gctgctcgct
      481 gtgcatgggc aaccaggcgc gggtagcgga gaatgcgacc gtggtatcga cgtcgacgcg
      541 taattttccg aaccggctcg gcaagggcgc cgatgtatac ctggcctcgg cggaactcgc
      601 cgcggtggcc gcgattctcg gtcgcatacc gaccatgggc gagtacctgc agtacgccgc
      661 cgatatcgac accctggccg acgacgtcta tcgctatctc aacttcaacg agatggagaa
      721 ctacaccgcc gcggctgaca aggcgttccc ggtcaaggtc gcctagcgga tcccgcggtc
      781 gggggggtgc gtcagccggc gggttcgccg cagtcgatga aacggcctcc gcgccggctc
      841 ggttctctgc tcgcgttctg ttgcctgttg tcggccggga ttccgccggc cgccgccgat
      901 ttcgtcggtg acttgatccg cgacttcgcc gatagcgaga tcgtgtttac ccgctcgcag
      961 tcgaacgtgc cgttcgtgcc gctggcctat gccgatctca gcctgtattc cgatacccgg
     1021 gtgcggcgac ccgacgactc ctcactggag ttcgatctgg cgaccgtctc ccagggggcg
     1081 ctgttgccgc tgctgttgag cccgcgcgac gccctgctgg tcggtgagtg ggccgccgtg
     1141 tcgaggttcg aagcgagcag cgcggatgcc gattccttca acgcactttc cgccggcctg
     1201 cccgtcggct ggttgcggca gatcgacgac caccgtcagt tcggcgcgtt tgtcatgccg
     1261 ctggcacacc gcgcggatct cgtaaattcc agttggagct acgaggtcct gggaggcgct
     1321 ttcgggcgtc acgaacgcga ccaacgcctc tggtgggtct acggtttcta tttcgatgtc
     1381 gggccgggcg acgatatcta cctgccttac ctgggcgcct cgtgggaatt cgacgacgag
     1441 ttgaccctga gcgcaatact gccctggccg gcgattctct atgcgcccga ccgcgatacc
     1501 ctgtggcgct tcggggcggc gccttccggc gcctcctggt cgctcgattc ggagcaaaac
     1561 gagatatcgt tttcactcga caactggcaa ctcggtttga cggccgagca ccgagtgtcc
     1621 ggcaacctgt ggttcgcatt ggaggccggg gtggccgggc tgcgcagcct caggctcgag
     1681 gacggcaagt ggcgcagtgc gaaattcgat gtcgacgaaa gcgcctacct gcgagtcgca
     1741 ttcaattttc ggcccgaacc gccgcgatag ccgattccgg ccgacggctg ccgaggcgcg
     1801 caacggcccg cctcggggta cgaaacctgt ccggcggcgt ggccagcgat tcgggcagca
     1861 gcgtgatagc atgcggcggc gtcaaaagcg cactatactg tctgccgaat tgactgcccg
     1921 gtcctgctcg atgccgttcg attgcacgca aatatccgtt acccgcgtga cgcgaagccg
     1981 tctcgccgag gtcgatttct cgcaactcgg tttcggttcc gtgtttgccg atcacatgtt
     2041 cagccagcac taccgtgacg gcagctggca gcagccgcaa atcgtgccct atcaggcgct
     2101 ggcgctggag cccggtgtcg ccatgctgca ttacggccag accgtgttcg acggttacaa
     2161 ggcgtttcgc tgtgtcgacg gcgaggcgcg catctttcga ccggagatga acgcgaagcg
     2221 cctgtatcaa tcctgccagc ggctgtgcat tccggtcatg ccgctggacg aattgcagca
     2281 aatcatgatc gaggcgacgc gcaaactgat cgagatcgac cacgcctgga taccacgcga
     2341 gtgggggcat tcgctgtatg ttcggccgtt gatcatcgcc accgaggcga cgctcgaagt
     2401 gcgcgccgcc aacagctatc acttcatgat catgacttcg ccggt
//
LOCUS       DATCZV010000383         4141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_349536, whole genome shotgun sequence.
ACCESSION   DATCZV010000383 DATCZV010000000
VERSION     DATCZV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349536"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VIW27_08660"
     CDS             complement(<1..657)
                     /locus_tag="VIW27_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY5739782.1"
                     /translation="MSPDAQPRPKRVGSAFWRGYRDCSPFILVVAPFGLLFGAVASDA
                     GLNLLETMTMTILVIAGAAQFTALALLQEQAPTLIVIAAALAVNLRMAMYSAALVPHI
                     GSASLVKRALASYFVVDQVFAVSVRAFDFDDGLDTGQKIAFIFGAGSAIFPFWIGGTL
                     VGAFVGDTIPPEFSLDFAIPICFISITAPMMRSLPHLLAALVSIAAALALAWMPYYLW
                     L"
     gene            complement(654..1511)
                     /locus_tag="VIW27_08665"
     CDS             complement(654..1511)
                     /locus_tag="VIW27_08665"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY5739783.1"
                     /translation="MAAKNLDRLVSLARFIADHADQTLTLESLARRVHLSPSRLQRVF
                     KSVFGISPKKLQQAARSDRFKALLRDGADVTEAIYAAGYGSSSRLYENRMQNIGMTPK
                     RYRAGGAGESITWACRETLLGPLLMAATERGVCSAQFGDDCASLRTQLQAEFPRAELR
                     AFGGDSSPQLDRWIEALNAYLQNKQPRPEVPLDLRGTAFQVKVWEFLLGLQEGDVASY
                     TEVARGIDKPGAVRATASACAANRVAVLVPCHRILRADGGLGGYRWGLERKRALLDAE
                     RARKAPAPA"
     gene            complement(1639..2814)
                     /locus_tag="VIW27_08670"
     CDS             complement(1639..2814)
                     /locus_tag="VIW27_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739784.1"
                     /translation="MQHEVEDERRCWIAGRSFDGLNNLFRASAEPGDDLISSLKIMTV
                     HGIGSHVPGYSRRLINSLVSEFHLTRMAENVKTVPLAHPDYDGDLGILSVYRYMNDAQ
                     TNEVLFFELTWDSIIEEQVAIIDYDNGQEPAARRVPLNHTMKTFLNKSIPDALAYNTR
                     YREPIQIAVAQSACWMLTGKWEDLPNGEARYCDATRPDAWSRIDNSSIAIISHSLGSR
                     ISMDAIQATLLRVAAQPELREVSLVLKNKPVYLYMLSNQLPLLQVGQPLPEVHGQIGS
                     ICGADGRRRDERLIERLQIVAFSDPNDLFSYTVKPAYLERYVDSRLCPTITNVVVEVA
                     PVTKFLGAEAFANPQSAHSDYDTDSRVLKMLVSGFGPNHGRSEVRERCEFIEAIPEY"
     gene            complement(3165..3584)
                     /locus_tag="VIW27_08675"
     CDS             complement(3165..3584)
                     /locus_tag="VIW27_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739785.1"
                     /translation="MKSLIFIVSAVLLAGCATGYNPGYRFNKVEAANLAGAPLKNLSW
                     NVVGSGKTMNCSEVAPNSICNDYFPGRRYPLAGIELDWTHVDGERRAEVLNPPVPAYF
                     YTAFPLEIYLEIDAEGAIEAFYRQNTPDGGSGVIIPN"
BASE COUNT          792 a         1290 c         1303 g          756 t
ORIGIN      
        1 cagccacagg tagtagggca tccacgccaa tgcgagcgcg gcggcgatcg ataccagcgc
       61 cgcaagcagg tgcggcaggc tgcgcatcat cggtgcggtg atcgaaatga aacagatcgg
      121 aatcgcgaag tcgagcgaga attccggcgg gatcgtgtcg ccgacgaacg caccgacgag
      181 cgtaccgccg atccagaacg gaaagatcgc cgaacccgcg ccgaagatga aagcgatctt
      241 ctgtccggtg tccaatccgt cgtcgaaatc gaaggccctg acggagaccg cgaacacctg
      301 gtcgacgacg aagtaggacg ccaaagcacg tttgaccagc gacgcgctac cgatatgcgg
      361 caccagcgcg gccgagtaca tcgccatgcg caggttgacc gcgagcgcgg cggcgatcac
      421 gatcagcgtc ggcgcctgtt cctgcagcaa tgccagcgcg gtgaattgcg ccgcgccggc
      481 gatgacgaga atcgtcatgg tcatggtttc gagcagattg agcccggcgt cgctcgccac
      541 cgcgccgaac agcagaccga acggcgcgac tacgaggatg aacgggctgc aatcgcgata
      601 accgcgccag aatgccgatc cgacgcgttt cggccgcggt tgcgcgtccg ggctcaagcg
      661 ggcgccggcg ccttgcgggc gcgctccgca tcgagcagcg cgcgcttgcg ttcgaggccc
      721 cagcgataac cgccgagacc gccgtcggcg cgcagaatgc ggtgacaggg caccagtacc
      781 gcgacgcggt tggcggcgca ggctgacgcc gtggcgcgca ccgcgccggg tttgtcgatc
      841 ccacgggcca cttccgtgta gctggcaacg tcgccttcct gcagcccgag caggaattcc
      901 cacaccttta cctggaacgc ggtgcccctc agatcaagcg gcacctcggg gcgcggctgc
      961 ttgttctgca gataggcgtt gagcgcctcg atccagcgat cgagttgcgg cgacgaatcg
     1021 ccgccgaagg cccgcagctc ggcgcgcgga aactccgcct gtaattgcgt cctcagggag
     1081 gcgcagtcgt cgccgaactg cgcagagcaa acgccacgct cggtagctgc catcagcagc
     1141 ggaccgagca gagtctcgcg acaggcccag gtgatgctct cgccggcacc accggcgcgg
     1201 tagcgcttcg gcgtcatgcc gatgttttgc attcggttct cgtacagccg gctgctcgac
     1261 ccgtagccgg ccgcgtagat cgcctcggtg acatcggcgc cgtcgcgcag cagcgccttg
     1321 aagcggtcgc tgcgcgccgc ctgctgcaac ttcttcgggc tgataccgaa caccgatttg
     1381 aacacccgct gcaggcgcga cggcgacagg tgcacgcgac gcgccagcga ttccagcgtc
     1441 agggtttgat cggcatggtc ggcgatgaaa cgggcgagcg agacaagtct gtcgaggttt
     1501 tttgcggcca tgatttgatc ccatactcca tcgcaatcga acatgctcga agcctagcgt
     1561 gactgaggcc gccatactct ccgaatattg tcgtcttcgc agcgcagacg aataccgttc
     1621 ggcgatcggg tcaagcgcct agtactcggg gatcgcctcg atgaattcgc agcgctccct
     1681 gacttcgctg cggccgtgat tcggtccgaa ccccgagacc agcattttca acacgcggct
     1741 atcggtatcg taatcactat gcgccgattg tgggttggca aaagcctcgg caccgagaaa
     1801 tttggtgacc ggcgccactt cgacgaccac gttggtaatc gtcggacaca gccgggaatc
     1861 gacgtagcgc tcgaggtagg ccggcttgac ggtgtaactg aacaggtcgt tggggtcgct
     1921 gaaagcgacg atctgcagac gttcgatcag ccgctcgtcg cggcggcggc catcggcgcc
     1981 gcaaatcgag ccgatctgac cgtggacctc gggcagcggc tggccgacct gcaacagcgg
     2041 cagctggttg gacagcatgt aaaggtagac cggcttgttt ttcaatacca gggagacttc
     2101 gcgcaattcc ggttgtgcgg caacgcgcag cagggtcgcc tggatcgcgt ccatgctgat
     2161 gcggctgccg aggctgtggg aaatgatcgc gatgctgctg ttatcgatcc gcgaccaggc
     2221 gtcggggcgg gtggcatcgc aatacctggc ttcgccgttg ggcaaatcct cccattttcc
     2281 ggtcagcatc cagcaagcac tctgcgcgac cgcgatctgg atcggctcgc ggtatcgggt
     2341 gttataagcc agcgcatcgg gaatcgattt attgaggaag gtcttcatcg tgtggttcaa
     2401 cggcacgcgc cgggccgcgg gttcctggcc gttgtcgtag tcgatgatcg cgacctgctc
     2461 ctcgatgatc gaatcccagg tgagttcgaa aaacaacact tcgttggttt gcgcgtcgtt
     2521 catgtaacgg tataccgaca atataccgag atcgccgtcg tagtccgggt gggcaagcgg
     2581 caccgttttg acattctcgg ccatgcgggt gagatggaac tcggagacca ggctgttgat
     2641 caggcgccgg gaataccccg gcacgtgcga gccgataccg tgcacggtca tgatcttgag
     2701 gctcgatatc aagtcgtctc ccggctccgc cgaggcacgg aacaggttgt tcagcccgtc
     2761 aaaggaacgc ccggcgatcc agcaacggcg ttcgtcttcg acctcgtgct gcatgattgc
     2821 ctcggtgatt ccgcgagaga tagacgcgca gccggtcaat gccgcgagca taactgccag
     2881 acacaggtat tgaattggtc gccgcatatc gcttggcctt cgctcgaaat cgggttcgag
     2941 gcatatctta agccgcgaac gagcgagatg aaagaccggg ccgactcccg gcaagcggtc
     3001 gccgcggcga ccaccggaaa cctgaaccgc tgcgaataat caagtcgcca tcggccaccg
     3061 ccgtcggcta cccctcggcg ggcctgccga agccgagcgc aggccgaccg ccggcacggc
     3121 ccgcctggcc aggccacagg cgacttcccg ccgtctgccg ctgctcaatt ggggatgatg
     3181 actccggatc cgccgtccgg cgtgttctgc cggtaaaacg cttcgattgc gccctcggca
     3241 tcgatctcca ggtagatttc gagcggaaag gcggtgtaaa agtaggccgg tacgggagga
     3301 ttcaggactt ccgccctgcg ctcgccgtcg acatgagtcc agtcgagttc gatgccggcc
     3361 aacggataac ggcgccctgg aaaataatcg ttgcatatcg aatttggcgc cacctcgctg
     3421 cagttcatcg tcttgccgct gcccaccacg ttccagctca ggttcttcag gggcgcaccg
     3481 gcaagattgg cggcttcgac cttgttgaat cggtagccgg ggttgtaacc ggtcgcgcaa
     3541 cccgccagca gcaccgccga aacgatgaat atcagggatt tcatggatat ctactccatc
     3601 ttgaaggttc cagtcgtcta tggcagacaa tacgattcgg atcaagttcc gcaatcgcgt
     3661 cgcccgcctg ccgcatcgcc gcaaccgact gccggcagtt cgacgcatcg ccgacaaccg
     3721 gattttgtct cgcttcggcg aaggccagga gccgcaatga gcggctgatc gcgggatccg
     3781 ccgggcaaat ttacggctcc tggcgcctat tcgacagtgg cagaattggc tgcggggacg
     3841 gtcgtacgga gttgtctcgt catcatccga tcgtgcgcgc aagcggccag caggcgggtt
     3901 cggacttttt ccttcgcagc cgatctatag taagatcacg gctgctttgt caggctgcct
     3961 gtcgagtccg acaccggcgc tgcgaacctg ccgccggtcg acggcaagca ggtaacctat
     4021 tcacccccgc ggagtgcaac gacagtgatt tcattcgcat acctgagacc ggtggcactg
     4081 tttgccggca tctgcttgct gggcgcagca gcccatgccg acgagccgcg cgagatcggc
     4141 t
//
LOCUS       DATCZV010000384         2682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_243605, whole genome shotgun sequence.
ACCESSION   DATCZV010000384 DATCZV010000000
VERSION     DATCZV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243605"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VIW27_08680"
     CDS             <1..675
                     /locus_tag="VIW27_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEY5739786.1"
                     /translation="LDLLLSIVLPSIVLMRFSDEQDLGASGALVVALAFPIGLGIYEL
                     LRYRQTNYIAVLGLVSVLLTGGIGLLQLDARWLAIKEAAIPAVLGIAVLVSARLGYPL
                     IRVLLYNPKILDTPRISRILRERGVEQAFELRLSNATYLLAGTFFFSAVMNYLLAAWI
                     VTSPSGTAAFNEELGRLTLLSYPVIAIPSMLMMLGIFWFLWRSIRAYTGLALEEILAS
                     PPTDQP"
     gene            complement(672..983)
                     /locus_tag="VIW27_08685"
     CDS             complement(672..983)
                     /locus_tag="VIW27_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739787.1"
                     /translation="MPFNFPLFVIVIVISTIAQESRQGRYQIPADDSSPKWGKFEIFW
                     KVIEGLPAIAKPPASSGSSRFDDLSAWCSTTPPRATVSGAGRAGARTDATHRVGNRAI
                     D"
     gene            977..1774
                     /locus_tag="VIW27_08690"
     CDS             977..1774
                     /locus_tag="VIW27_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739788.1"
                     /translation="MAFHWFRIAAGLLLACNFAIAVADMPMPVDWKKPGDGLLTYDPA
                     TGLEWLDLSYTTNRSYNDIVSQLEPGGEFEGFRYASIAEITALWDHLGGVRPYSGASI
                     ENRDLFDIVAALFGEAVSCGVLRCIRWVSADTYPSNLFDARWVARSNSGANIFDSFWA
                     FPDGLRVELRSPLHGHALVRQVIADSAADRVLSDLDNCTAISGFRQSSADLCETQGSP
                     AVEPSGAGADGPANADRYLQKVAAPGAGDSAQSISVTRRPSGERSVD"
     gene            complement(1956..>2682)
                     /locus_tag="VIW27_08695"
     CDS             complement(1956..>2682)
                     /locus_tag="VIW27_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5739789.1"
                     /translation="TQRVRDDLYCITEPHYRWENRANLWLIRGREADLLIDTGLGVSS
                     LKLYLADLLDKPLKVVASHVHFDHSGGCHEFEEVYIHANEQRALCDGEQRLILSDPEL
                     GFVPERDFESIPYQDFSADEYAVQACPQARALAHGDVIDLGNRAFEVMHLPGHSSGSI
                     GLYNRAERQFFSGDVVYDGELLDALEDSVIDDYLDSMAKLLSLKTDEVRPGHYRSFDR
                     RRLRELVRLYIDSKKAPRCPADG"
BASE COUNT          474 a          862 c          812 g          534 t
ORIGIN      
        1 ctcgacctgt tgctgagtat agtcctgccg tcgatcgtgt tgatgcgctt cagcgacgaa
       61 caggatctcg gcgcgagcgg cgcgctggtc gtggcgctgg catttccgat cggcctcggt
      121 atctacgaac tgctgcgcta tcggcaaacc aactatattg cggtgctcgg cctggtcagc
      181 gtcctgctca ccggcggcat cggcttgctg caactcgacg cccgctggct agcgatcaag
      241 gaagccgcga ttcccgcggt gctcggcatc gcggtgctgg tctcggcgcg cctcggttac
      301 ccgttgatca gggtcttgct gtacaacccg aagattctcg acaccccgag aatttcgcgc
      361 atcctgcgcg agcgaggcgt cgagcaagcc ttcgagttgc gcctgtcgaa tgccacttac
      421 ctgctggccg ggacgttttt cttttccgcg gtgatgaatt acctgctcgc cgcctggatc
      481 gtcaccagcc catcgggaac cgcggccttc aacgaggaac tcgggcgcct gaccctgctc
      541 agctacccgg tcatcgcaat cccgtcgatg ctgatgatgc tcggcatttt ctggttcctg
      601 tggcgcagca ttcgtgccta caccggtctc gcgctcgagg agatcctcgc ctcgccgccc
      661 accgaccagc cctagtctat cgctcgattc ccaacccggt gcgtagcatc cgttcgagcg
      721 ccggcacgac ccgcgccgct aactgttgcc cgcggcgggg tggtcgaaca ccacgcgctt
      781 aaatcatcga agcggctcga gccgctgctc gccggcggct tggcgatcgc cggcaggccc
      841 tcgatcacct tccagaatat ctcgaattta ccccatttgg gtgatgaatc atctgccgga
      901 atttgataac gtccctgccg ggattcctgc gcgatcgttg atataacaat aacaatgacg
      961 aagaggggga aattaaatgg catttcactg gtttcgcatc gcggccggct tgctgctggc
     1021 ctgtaatttt gccattgcgg ttgccgacat gccgatgcct gtcgactgga aaaaacccgg
     1081 cgacggcctg ctgacctacg atccggccac cgggctggaa tggctcgacc tgtcgtacac
     1141 caccaatcga tcgtacaacg atatcgtcag tcaactggag ccgggcggtg aattcgaagg
     1201 tttccgctac gccagcatcg ccgagatcac cgccctgtgg gatcatctcg gcggggtccg
     1261 gccctacagc ggagcatcga tcgaaaaccg ggatctcttc gatatcgttg ccgcattgtt
     1321 cggcgaggcg gtcagttgcg gtgtactcag atgtattcgc tgggtctcgg cggacaccta
     1381 cccgagcaac ctgttcgacg ctcgctgggt agcacgctcg aatagcggcg cgaacatctt
     1441 cgatagtttc tgggcatttc ccgatggcct gcgcgtcgag ctgcgcagtc cgttgcatgg
     1501 ccacgctctg gtacggcagg tcatcgcgga ttccgctgcc gaccgcgtcc tcagcgacct
     1561 cgacaattgc acggcgattt ccggcttccg ccagtcgagc gccgacctct gtgaaacgca
     1621 gggttcgccg gccgtggagc ccagcggtgc gggcgcagac ggaccggcaa acgcggaccg
     1681 atacctgcaa aaggtcgccg cccccggagc cggggattcc gcccagtcga tatcagttac
     1741 gcggcgcccc tcgggcgagc ggtctgtcga ttagccgctc tcaggcaata gcaggcaacc
     1801 ggctcgctga cgaaccgcgg cgctgcgacg taaaggcgtc gacctcgatc tggcgagacc
     1861 tgcagcgacc aataattcct gccggcgtcg cctgacaacc ggggtagcga ccagctcgtc
     1921 caccggatct gcccgcacag caaccgcccg gcagctcagc cgtcggccgg gcagcgcggc
     1981 gccttcttgc tgtcgatgta taacctgacg agttcgcgca ggcggcggcg atcgaaactg
     2041 cggtaatgcc cgggcctgac ctcgtcggtc ttcaggctca gcagttttgc catcgagtca
     2101 aggtaatcgt cgatcaccga gtcttcgagg gcatcgagca gctcgccgtc gtagaccacg
     2161 tcgccggaaa agaattgccg ctcggcccgg ttgtacaagc cgatggaacc cgacgaatgc
     2221 ccgggcaggt gcatgacctc gaacgcccgg ttaccaagat cgatgacgtc gccgtgggcc
     2281 agcgcccgag cctgcgggca ggcttgaacc gcgtattcgt cggcgctgaa atcctggtat
     2341 ggaatactct cgaaatcgcg ctcgggcacg aagccgagct cgggatcgct gaggatcagc
     2401 cgctgctcgc cgtcgcacag cgcgcgctgc tcgttggcat ggatatacac ctcttcgaac
     2461 tcatgacagc cgccgctgtg atcgaagtgc acgtggctgg cgaccacctt tagcggcttg
     2521 tcgagcaggt cggcgaggta cagcttgagg ctcgacaccc cgagaccggt atcgatcagc
     2581 aggtcggctt cgcggccgcg aatcagccac aggttggcgc ggttttccca ccggtagtgt
     2641 ggttcggtga tgcaatacag atcgtcgcgt acgcgctgtg tc
//
LOCUS       DATCZV010000385         4063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_773729, whole genome shotgun sequence.
ACCESSION   DATCZV010000385 DATCZV010000000
VERSION     DATCZV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773729"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VIW27_08700"
     CDS             <1..1073
                     /locus_tag="VIW27_08700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5739790.1"
                     /translation="NLISDSLLLQEAARRGITITDTQLNQTMQRLARQNSMSLSEFRE
                     RLLADGLDYDEYRETVRRELTISSLGRQYGQRTASISDAEVDDFMARSGADDIDYEYR
                     ISHILIALPDAASPELISAARETADEILARLDQGEEFDRLANTFSAGDTALQGGDLGW
                     RKQAQIPSLFTDSVLAMEPGDHAGPLRSASGLHIVHLKERRNLEQVLSEQTRSRHILI
                     RPNELISEEDTKNRLLEFRQRILDGEDFGRLARLYSVDYTSGSEGGDIGWTDRGATVK
                     EYQSVTDSLEMGEISEPFRTQFGWHIVEVTGRRTVDETAQSKREKIYSQLLEQKQREV
                     FDLWKRRLRDEAYVVFPDRPDA"
     gene            1066..2049
                     /gene="pdxA"
                     /locus_tag="VIW27_08705"
     CDS             1066..2049
                     /gene="pdxA"
                     /locus_tag="VIW27_08705"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY5739791.1"
                     /translation="MPDGPIIITSGEPAGIGPDIVAAIDPARFDARLVVIGNRELLEV
                     RAAATGSKISYVEYGSPQGSNNGVEVIDQPLARPCRAGHLDEANAAYVLTMLERACRG
                     CLDGEFAAMVTAPVQKEIINRAGLAFSGHTEYLAEICGAPRPVMLLIAGSLRVALVTT
                     HLPLRAVADAISAEAVREVIEILARDLRHRFAIADPLIKVCGLNPHAGENGFLGREEL
                     DIIIPALDELRAAGIRLGGPYPADTLFTAHMLADADAVLAMYHDQGLPVLKHVGFHNA
                     VNTTLGLPIIRTSVDHGTALELAGSGRARADSLFAALDSAIEQARHLKSAA"
     gene            2053..2826
                     /gene="rsmA"
                     /locus_tag="VIW27_08710"
     CDS             2053..2826
                     /gene="rsmA"
                     /locus_tag="VIW27_08710"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY5739792.1"
                     /translation="MARPRKRFGQHFLQDSQIIDRILRAVDPQPGETLLEIGPGQGAL
                     TLPLLRRCGQLIAVEIDRDLVPVLKSQAAGVGDLEIVNADILAFDLANIGLQGGLRLV
                     GNLPYNISTPLMFHLLDSARLIRDMHFMVQKEVAQRAVAAAGDSSYGRLSVMLQYRCR
                     CHYLFDVPPASFRPPPKVDSAVIRLEPYPEPQHEIGDYTVFSKLVQAAFGQRRKTVSN
                     SLKSMLDRATIAACGIDPGLRAENLAIADFARLSRAASQ"
     gene            2823..3320
                     /gene="folA"
                     /locus_tag="VIW27_08715"
     CDS             2823..3320
                     /gene="folA"
                     /locus_tag="VIW27_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HEY5739793.1"
                     /translation="MTRLSLIVAMDRNRLIGSANGLPWQLPADLAFFKRTTMGKPVVM
                     GRKTFESIGKPLPGRQNIVVTRDSAFVADGCVVVGSVEQAIESCAASPEIMLIGGASL
                     YRQTLERADCLYVTRIDHEFDGDTWFPEFDESLWRIESHEDFEADSRNPYACSFVKYV
                     RENSI"
     gene            3395..3682
                     /locus_tag="VIW27_08720"
     CDS             3395..3682
                     /locus_tag="VIW27_08720"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5739794.1"
                     /translation="MGSKRYTIGREGQIYINDPTVSKQHAEIQITDGEVYLRDLGSTN
                     GTFLIKNNRLVPFREGYVQLHQPIVLGNRQYTIRSLLEIAGVFARQHDAPQ"
     gene            complement(3710..>4063)
                     /locus_tag="VIW27_08725"
     CDS             complement(3710..>4063)
                     /locus_tag="VIW27_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739795.1"
                     /translation="GIRGTDYVVKLCRLDDCTRTASRGDLDAKLHAVVLEGAISLTGD
                     EQVQLPIAAGEYGTATVESLVVEQTAAIPPGLLTAEEARRVDVPAPQPGAVEDQQASG
                     AWKWILAIMLLVVSL"
BASE COUNT          765 a         1371 c         1240 g          687 t
ORIGIN      
        1 aaaacctgat cagcgattcg ctgctgttgc aggaggccgc gcgccgcggc atcacgataa
       61 ccgataccca gctgaaccag accatgcagc gcctggcgcg gcagaattcg atgagcctgt
      121 cggagtttcg cgagcgcctg ctcgccgacg gactggacta cgacgaatac cgcgaaaccg
      181 tgcgccgcga gctgacgatt tcatccctcg gccgccagta cggccagcgc actgccagca
      241 tcagtgacgc cgaggtagac gacttcatgg cccgcagcgg cgcagacgac atcgattacg
      301 aataccgcat ctcgcatatc ctgatcgcgc tgcccgatgc cgcctccccg gagctgatca
      361 gcgcggcgcg cgagaccgcc gacgaaatcc ttgcgcggct cgaccagggc gaggaattcg
      421 atcgcctggc gaataccttc tcggccggcg acaccgcgtt acagggcggc gatctcggct
      481 ggcgcaagca agcccagatt ccgagcctgt tcaccgatag cgtgctggcg atggagcccg
      541 gcgatcacgc cggcccgttg cgcagtgcca gcggcctgca catcgtgcac ctcaaagagc
      601 gccgcaatct ggagcaggtg ctcagcgagc agactcgcag ccggcatatc ctgattcggc
      661 ccaacgaact gatctccgag gaagacacca aaaatcggct gctcgaattt cgccagcgaa
      721 tcctcgacgg cgaggatttc ggacgactgg cgcgactcta ctcggtcgac tacacctcgg
      781 gctccgaggg cggcgacatc ggctggaccg accgcggcgc caccgtcaag gaataccagt
      841 cggtcaccga cagcctcgag atgggcgaga tcagcgaacc tttccgtacc cagttcggct
      901 ggcacatcgt cgaggtcacc ggccgccgca cggtggacga gaccgcgcaa agcaagcgcg
      961 aaaagatcta ttctcaactg ctcgagcaga aacagcgcga agtgttcgac ctgtggaaac
     1021 gccgcctgcg agacgaagcc tacgtcgtat tccccgaccg gcccgatgcc tgacgggccg
     1081 atcattatta cctccggcga gcccgcgggc atcggccccg acatcgtcgc cgcgatcgac
     1141 cccgcccgtt tcgatgcccg cctggtggtg atcggcaacc gcgagctgct ggaagtgcgc
     1201 gccgcggcca cgggttcgaa aatctcctat gtcgaatacg gtagcccgca ggggtcgaat
     1261 aacggcgtcg aagtcatcga tcagccgctg gcgcgaccgt gccgggccgg gcacctcgac
     1321 gaagccaacg ccgcttacgt gctgacgatg ctggagcgcg cctgccgcgg ctgcctcgac
     1381 ggcgaattcg ccgccatggt gaccgccccg gtgcaaaagg aaattatcaa ccgtgccgga
     1441 ctcgcttttt cgggacacac cgagtacctg gcggagatat gcggggcacc gcggccggtg
     1501 atgctgctga tcgccggcag cctgcgggtg gcgctggtca ctacccacct gccgctgcgt
     1561 gcagtcgccg acgcgatcag tgccgaggcc gtgcgcgagg taatcgaaat cctcgcccgg
     1621 gacctgcgcc accgtttcgc tatcgccgac ccgttaatca aggtttgcgg cctcaatccg
     1681 cacgccggcg aaaacggctt tctcgggcgc gaggaactcg acatcatcat tccggcgctc
     1741 gacgagctgc gcgccgcggg tattcgcctt ggcggcccct acccggcgga cacgctgttc
     1801 accgcgcata tgctcgccga tgccgacgcg gtgctggcga tgtatcacga ccagggcctg
     1861 ccggtgctca agcacgtcgg ctttcacaac gcggtcaata ccacgctcgg cctgccgatc
     1921 atccgcacct cggtcgatca cggtaccgcg ctcgagctgg ccggcagcgg ccgcgcgcgc
     1981 gccgacagcc tgttcgcggc gctcgactcg gcgatcgagc aggcacgcca cctgaaaagc
     2041 gcggcctgat tcatggcccg tccgcgcaaa cgcttcggcc agcacttcct gcaggattcg
     2101 cagatcatcg atcgcatcct gcgcgcggtc gacccgcagc ctggcgagac gctgctggag
     2161 atcggtcccg gccagggtgc actaaccctg ccgctgctgc ggcgttgcgg gcagctgatc
     2221 gcggttgaaa tcgaccgtga cctggtcccg gtcctgaaaa gccaggcggc cggcgtcggc
     2281 gatctcgaaa tcgtcaacgc cgatattctc gctttcgatc tcgcgaatat cggcctgcag
     2341 ggcggtttgc ggctggtcgg caacctgccc tacaacattt cgacgccgct gatgtttcac
     2401 ctgctcgatt cggcgcggct gatacgcgac atgcatttca tggtgcaaaa ggaggtcgcg
     2461 caacgcgctg tcgctgccgc cggcgactcc agctatggcc ggctgtcggt gatgctgcag
     2521 taccgctgcc gatgccatta ccttttcgac gtgccgcccg ccagtttcag gccgccgcca
     2581 aaggtggact cggcggtgat tcggctcgag ccctaccccg aaccgcagca cgagatcggc
     2641 gattacacgg tattctcgaa actcgtgcag gcggccttcg gccagcgccg caagaccgtt
     2701 tccaactcgc tgaagtcgat gctcgaccgc gccacgatcg ccgcctgcgg catcgatccc
     2761 ggcctgcggg ccgagaacct cgcgatcgcc gatttcgccc gcctgagcag ggccgccagc
     2821 caatgacgcg gctttcactg atcgttgcga tggaccggaa tcgcctgatc ggcagcgcca
     2881 atggcttgcc gtggcagctg ccggcggacc tggcattttt caaacgcacc acgatgggga
     2941 aaccggtggt catgggccgc aagactttcg aatcgatcgg caagccgttg ccggggcggc
     3001 aaaatatcgt cgtcacccgc gatagcgcgt ttgtcgccga cggttgcgtg gtcgtcggca
     3061 gcgtcgagca ggccatcgag tcatgcgcgg catcgccgga aatcatgctg atcggcggcg
     3121 ccagccttta ccgccagacc ctggagcggg cggactgcct gtacgtaact cgaatcgatc
     3181 acgagttcga cggcgacacc tggttccctg aattcgacga aagcctctgg cgaatcgagt
     3241 cccacgagga tttcgaggcc gacagccgaa atccctatgc ctgctcgttc gtcaaatatg
     3301 tccgcgaaaa tagcatataa ttggggtaga ctttagcgcc ccgcaggcgg ggcggtcacc
     3361 gcaaaacaaa cagccaggca cgaagatcga gcgaatgggg agcaagcgtt ataccatcgg
     3421 acgcgagggt caaatctata tcaacgatcc tacggtcagc aagcagcacg ctgaaatcca
     3481 gatcacggac ggcgaggtat acctgcgcga tctgggttcg accaacggca ctttcctgat
     3541 caagaacaac cgcctggtac ccttccgcga gggttacgtg cagctgcacc agcccatcgt
     3601 gctcggcaat cgccagtaca cgattcgcag cctgctggaa atcgccggcg tatttgcgcg
     3661 gcagcacgac gcgccgcaat aatccgcgcc cggtgccgcc ggcggcggct tacagactga
     3721 caaccagcaa catgatcgcc agaatccact tccaggcccc ggaagcctgc tgatcctcga
     3781 ccgcgcccgg ttgcggtgcc gggacgtcca cgcggcgcgc ttcctcggct gtcagcagcc
     3841 ctggcggaat cgcggccgtc tgttcgacga ccagcgactc gacggtcgcg gtgccgtatt
     3901 ctccggctgc tatcggcagc tgcacctgct cgtcaccggt cagcgatatc gcgccttcga
     3961 gcaccacggc gtgcagcttg gcatcaagat cgccgcggct cgcggttcgc gtgcaatcgt
     4021 ccagccggca cagcttgacg acgtaatccg tgccgcgaat acc
//
LOCUS       DATCZV010000386         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_667446, whole genome shotgun sequence.
ACCESSION   DATCZV010000386 DATCZV010000000
VERSION     DATCZV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667446"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VIW27_08730"
     CDS             <1..301
                     /locus_tag="VIW27_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5739796.1"
                     /translation="LLLMALGSCTSMTLRMYADRKQIPLDDLQVHLRHDRVHAADCAD
                     CEQGKGMERITRRIKLGGELDRGQREKLLEIADKCPVHRSLEADLRIVSELGDQD"
     gene            complement(298..1257)
                     /locus_tag="VIW27_08735"
     CDS             complement(298..1257)
                     /locus_tag="VIW27_08735"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEY5739797.1"
                     /translation="MKPTSIGAAAATLLLLGCAQANAGAGFASREMNPVLQSIYLPAL
                     VPMSSDAGWRIDHSLYLTNTLQDMGSGDEELLIDAESYRYEFALRNRRGNWVTQITVP
                     LVATEGGQLDSVIDDWHDFFGLPHGRRDRFARDNLDIEYRRDGDVQFSQTESSSGLGD
                     LSLAIGYQPAGETGYYVGVDLPTGSADDFSGNEKTDVALWMQRTLEFATDTRAYGMLG
                     VSFPGDGGKLEGLLVERVWAAQLGMEFRFTDNLLATAQLDMHSRTIDGSQLDAFGNSL
                     QILLGLGFANLWDEYRLDLFFTEDILVGSAPDISFGLRLAREF"
     gene            complement(1244..>2306)
                     /locus_tag="VIW27_08740"
     CDS             complement(1244..>2306)
                     /locus_tag="VIW27_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY5739798.1"
                     /translation="VFHLLNDSVHLFSAGDADKISSGEVIEGLDNPRLDEILGATADD
                     FREEIDLVRGATNEFDLDAYLAGELTPVFFGSAINNFGMAELLDAFLAYAPPPTPRPT
                     RSRMVAPDEPQLTGFVFKIQANMDKQHRDRIAFMRICSGKYTRGMKVRHVRIGKDIKI
                     PDALTFFAADRGRIDEAYTGDIIGIHNHGTIRIGDSFSQGEQLAFTGIPNFAPELFRR
                     ARLKDPLKMKALLKGLIQLCEEGATQLFRPVANNDLILGAVGVLQFDVVAHRLKDEYG
                     VDCQFEAVNVNTARWITSDDSKKLEEFKNKMRANLAYDHAGDLAYVAPSRVNLTMTEE
                     RWPEIEFLATREHGDYETD"
BASE COUNT          423 a          692 c          751 g          440 t
ORIGIN      
        1 cctgctgttg atggcactcg gctcatgcac ttcgatgacg ctgcgcatgt atgccgatcg
       61 caagcaaata ccgctcgatg acctgcaggt tcacctgcga cacgatcgcg tgcacgcggc
      121 ggactgtgcg gattgcgagc agggcaaggg aatggagcgc attacgcgac gcataaaact
      181 gggcggtgaa ctcgaccgcg ggcagcgcga aaaactgctg gagatcgccg acaagtgccc
      241 ggtgcaccgc agcctcgaag ccgatctgcg aatcgtcagc gaactgggcg accaggacta
      301 aaattcgcgc gccaggcgca ggccgaaact gatgtcgggc gcgctgccga ccaggatgtc
      361 ctcggtgaag aacaggtcga ggcgatactc gtcccacagg ttggcaaagc cgagcccgag
      421 cagtatctgc agggagttgc cgaatgcatc cagctggctg ccgtcgatgg tacgcgagtg
      481 catgtcgagc tgcgcggtcg ccagcaggtt gtcagtaaag cggaactcca tgcccagctg
      541 ggcggcccag acgcgctcga ccagcaggcc ctcgagtttg ccgccgtcgc cgggaaagct
      601 gacgccgagc atgccgtagg ctcgagtatc ggtggcgaac tccagcgtgc gctgcatcca
      661 cagcgcgaca tcggtcttct cgttgccgct gaaatcgtct gccgagccgg tcggcaggtc
      721 gacgccgacg taatacccgg tctcgcctgc cggctggtaa ccgatcgcca gcgacaggtc
      781 gccgaggcca ctgctggatt cggtctgcga aaactgcaca tcgccgtcgc ggcgatactc
      841 gatatcgagg ttgtcgcgcg cgaatctgtc gcgcctgccg tgcggcaggc cgaagaaatc
      901 gtgccaatca tcgatcacgg agtcgagttg gccgccttcg gtggcgacca gcggtacggt
      961 aatctgcgtc acccagttac cgcgccgatt gcgtaacgcg aactcgtagc ggtaggattc
     1021 cgcgtcgatc agcagttcct cgtcgccgct gcccatgtcc tgcagcgtgt tggtgaggta
     1081 aaggctgtga tcgatgcgcc agcccgcgtc gctgctcatc ggcaccagtg ccggcaggta
     1141 tatcgactgc agtaccgggt tcatctcgcg gctggcgaat ccggccccgg cgttggcttg
     1201 cgcgcagccc agcagcagca gggtggcggc ggccgcgccg atactagtcg gtttcatagt
     1261 cgccatgctc gcgggtggcg agaaactcga tttccggcca gcgttcctcg gtcatcgtca
     1321 gattgacgcg cgatggcgcg acataggcga gatccccggc atgatcgtag gcgaggttgg
     1381 cgcgcatctt gtttttgaat tcctcgagct tcttgctgtc gtcgctggta atccagcgcg
     1441 cggtgttgac attgaccgct tcgaactggc aatcgacgcc atactcgtcc ttgagccggt
     1501 gggcgacgac gtcgaattgc agcacgccga cggcgccgag aatcaggtca ttgttggcga
     1561 ccggcctgaa caattgcgtc gcgccttcct cgcaaagctg tatcaggccc ttcagcaggg
     1621 ctttcatttt cagcggatcc ttgagtcgcg cacgccgaaa cagctccggc gcaaagttcg
     1681 gaataccggt gaacgccagc tgctccccct ggctgaaact gtcaccgatg cgaatcgtgc
     1741 cgtgattgtg aatgccgatg atatcgccgg tataggcttc gtcgatacgg ccgcggtcgg
     1801 cggcgaaaaa ggtcagcgcg tcgggtatct tgatatcctt gccgatgcgc acgtggcgga
     1861 ctttcatgcc gcgcgtgtac ttgccggagc agatgcgcat gaaggctata cggtcgcggt
     1921 gctgcttgtc catgttggcc tggatcttga acacgaagcc agtcaactgc ggttcgtcgg
     1981 gggcgaccat gcggctgcgc gtcggccgcg gggtcggcgg cggcgcatag gcgaggaacg
     2041 catcgagcag ttcggccatg ccgaaattgt tgatcgccga gccgaagaat accggcgtca
     2101 gttcgccggc gaggtaggca tcgagatcga actcgttggt ggcgccgcgc accaggtcga
     2161 tttcctcgcg gaagtcgtcg gccgtggcgc cgagaatttc gtccagcctg ggattgtcca
     2221 gcccttcgat aacctcgccc gagctgatct tgtcggcgtc gccggccgag aacaggtgca
     2281 ccgaatcgtt gagcaggtgg aagacg
//
LOCUS       DATCZV010000387         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_296173, whole genome shotgun sequence.
ACCESSION   DATCZV010000387 DATCZV010000000
VERSION     DATCZV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296173"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VIW27_08745"
     CDS             complement(<1..308)
                     /locus_tag="VIW27_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5739799.1"
                     /translation="MTDRRNFLKGMGAAAAIGAGLVSRSALAALPESPTSDATTLQVP
                     PEPTTGPWYQPVVTLNSWSLPWRMKNGWKEFHLTAEAVEREIAPGMTARLWGYNGQSP
                     G"
     gene            complement(321..1727)
                     /locus_tag="VIW27_08750"
     CDS             complement(321..1727)
                     /locus_tag="VIW27_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5739800.1"
                     /translation="MKITVKLTRLSFIGLIVSTAACTSIPDDAGVGPVQELVDAQIGT
                     DSPASRLEPENMFPPEKVSSLLDAPLSVDGAELLSMQLNPLVRSNLLRVGIAEADYAQ
                     AGRLRNPGFTYERSEEGEYGASLLFDIGGLLLMPLRREVELRRLEAARFDAAGEVISH
                     LANTRMAWIRAVTEKQLTELMERSLESTATANDMTRQMTALGHSAAMEAAESEIFLGE
                     MRSALTRQRLAETAAREALIRQLGLWGDAVRKLTLPSRLPALPESQLDYPSVEGEAIA
                     GRLDVQIAKATIEAMASNYSLTRRSPFLSSIEFGPVMEKGDEDTEYGYELEFSIPIFD
                     AGGIANEKARFLFEQSQDQARSVAISAASEAREAYQTYRSYWDIARYYENVMLPLRQQ
                     VSEERLLHYNGMLISVFDLLDDVRAAADLEASAIRAVRDFWLADARLQLALTGSSPGM
                     AMNTSVETPAAGGADADH"
     gene            complement(1724..2980)
                     /locus_tag="VIW27_08755"
     CDS             complement(1724..2980)
                     /locus_tag="VIW27_08755"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY5739801.1"
                     /translation="MKSSFYAIVCAGCVLTISPGLQAESKVPALDPGQHPETMRYESL
                     FPDAMVPLDTELSWKNRFLGNESFNRDESLPSTGPELESDAPDQSSAPGDGMPAGQLD
                     ATGVIKQIKASEGKLKIAHGPIERLGMPAMTMMFRVEDPARLEGLSKGDEVAFNVDNT
                     AAGFSITRLETAGGSSGKAFDASGTIRSIRASQGKVKIEHGPIDRLGMPGMTMVFKLQ
                     DTDALQNLEQGMPVEFDVVNAPGGFEITRIQPVGASVAAPSTGVKRICYSIGPFLQQA
                     RAVALSRRYREQGASSRVETRAEREYLGDMVYIDGHASPAAALATASDLAAKGIGDSF
                     VLDLPGKPNVLSLGVFSQQQNANRLKARVKALNYSVKTEARYRQRTTYWLHNEQSGGT
                     PPKLLSAGDVEAGVREISGECAGEDS"
     gene            complement(3085..3444)
                     /locus_tag="VIW27_08760"
     CDS             complement(3085..3444)
                     /locus_tag="VIW27_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739802.1"
                     /translation="MNAQKMAPYGSKVARRVSRLMLAAILLLNTAAIGAMELQSTLLS
                     QPGEHSTPCHMTGEMGGHHDEHTLGCDSLCAQCTGFIPGIFAAPSERMSSTARFPLTG
                     TDPVPQYQTRLYKPPRA"
BASE COUNT          767 a         1258 c         1316 g          856 t
ORIGIN      
        1 cccggcgact ggccgttgta gccccagagg cgggcggtca tgccgggcgc aatctcgcgc
       61 tccaccgcct ctgcggtgag gtgaaattcc ttccagccgt ttttcatgcg ccacggtagc
      121 gaccagctgt tgagcgtcac caccggctgg taccagggcc ccgtggtcgg ttcgggcggt
      181 acctgcaacg tggtggcgtc ggaagtcggc gattccggca acgcggccag cgcggaacgc
      241 gataccaggc cggcgccgat cgcggccgcg gcgcccattc ccttgagaaa atttcttctg
      301 tcagtcatta cggtaatcct ctagtgatcc gcgtcggcgc cgcccgcggc gggtgtttcg
      361 acactcgtgt tcattgccat gccgggagac gagccggtca gggccagctg cagccgcgcg
      421 tcggcgagcc agaagtcgcg caccgcgcga atcgcgctgg cctcgagatc ggccgccgcg
      481 cgcacgtcgt cgagcaggtc gaagacgctg atcagcatgc cgttgtaatg cagcagccgt
      541 tcctcgctga cctgttgccg cagcggtagc atgacgtttt cgtagtagcg cgcgatatcc
      601 cagtaactgc ggtaggtctg gtatgcttcg cgcgcctcgg aggcggccga gatcgcgacc
      661 gaccgggcct ggtcctgcga ctgctcgaac agaaacctgg ccttttcgtt ggcgatgccg
      721 ccggcgtcga atatcgggat gctgaattcg agctcgtaac cgtactcggt atcttcatcg
      781 cctttttcca tgaccggacc gaactcgatc gaggacagga aagggctgcg gcgtgtcagc
      841 gaatagttgc tggccatggc ctcgatcgtc gccttcgcga tctgtacgtc gagccgtccc
      901 gcgatcgcct cgccttcgac cgagggatag tcgagctgcg attccggcag cgcgggcagg
      961 cgcgacggca aggtcagctt gcgcacggcg tcgccccaca ggccgagctg acgaatcagg
     1021 gcttcgcgcg ctgcggtttc tgccagccgt tgccgggtca gcgcagagcg catttcaccg
     1081 aggaaaattt ccgactcggc ggcttccatc gcggcactgt ggccgagcgc ggtcatttgc
     1141 cgggtcatat cgttggcggt agcggtcgac tccagcgagc gttccatcag ttccgtcagc
     1201 tgtttttccg ttaccgcgcg gatccaggcc atgcgcgtgt tcgcgaggtg cgagatgact
     1261 tcgccggcgg catcgaagcg cgccgcctcg agccggcgca gttcgacttc gcggcgcaac
     1321 ggcatcagca gcaggccgcc gatgtcgaac agcagcgaag caccgtactc gccttcctcg
     1381 gaacgctcgt aagtaaagcc cggattgcgc aggcgcccgg cctgggcgta gtcggcctcg
     1441 gcgatgccga cccgcagcag gttggagcgc acaagtggat taagctgcat cgacagcagt
     1501 tcggcaccgt cgaccgaaag cggcgcgtcc agcaggctgg aaaccttctc gggcgggaac
     1561 atgttttcgg gctcgagccg gctcgcggga ctgtccgtac caatctgggc atcgaccagt
     1621 tcctgcaccg ggccgacgcc ggcgtcatcg ggaatactgg tacaggccgc cgtgctgacg
     1681 atcagcccga taaacgacag tcgggtcaat ttcaccgtga tcttcatgaa tcctccccgg
     1741 cacactcgcc gctgatctca cggacaccgg cctcgacatc gccagccgag agcaatttcg
     1801 gcggcgttcc gccgctctgc tcgttgtgca gccagtaagt cgtgcgttga cgatagcgag
     1861 cttcggtttt aacgctgtag ttcaaagctt tgacacgtgc cttcaaacgg ttcgcgtttt
     1921 gctgctggct gaacacgccg agcgacagaa cattgggctt gcccggcagg tcgagtacga
     1981 agctgtcacc gatgcctttt gccgccagat cgctggcggt ggcgagcgcg gcagcgggcg
     2041 atgcatggcc gtcgatatag accatgtcac cgagatactc tcgctcggcg cgggtctcga
     2101 cccgggagct ggcgccctgt tcgcggtagc gacgactcaa tgccaccgcc cgcgcctgct
     2161 gcaagaaggg tccgatcgag tagcagatgc gcttcactcc ggtcgacggg gccgcgacgc
     2221 tcgcgcccac gggctgaatg cgagtaatct cgaaaccgcc cggggcgttg acgacgtcga
     2281 attcgaccgg catgccttgc tcgaggttct gcagtgcatc ggtgtcctgt agcttgaaga
     2341 ccatcgtcat gccgggcatg ccgagcctgt cgatcgggcc gtgttcgatc ttgaccttgc
     2401 cctgcgaggc tcgaatcgag cgaatcgtgc cgctggcatc gaaagccttg ccgctacttc
     2461 cgccggcggt ttcgaggcgg gtgatgctaa agccggcggc ggtattgtcg acgttgaaag
     2521 cgacttcgtc gcccttgctc aaaccctcca gccgggcggg gtcctcgacg cgaaacatca
     2581 tcgtcatcgc cggcatgccg aggcgctcga tcgggccgtg cgcgatcttg agcttgcctt
     2641 cggaggcctt gatctgcttg atcaccccgg tcgcatccag ctgccccgcg ggcatgccgt
     2701 caccgggggc gctagactga tcgggcgcgt ctgattccag ttccggtccg gtcgacggca
     2761 gcgattcgtc tcgattgaag ctctcgttgc cgagaaagcg gtttttccag gacagctcgg
     2821 tgtccagcgg caccatggca tccgggaaca gcgattcgta gcgcatcgtt tccgggtgtt
     2881 gccctgggtc gagcgctgga accttcgatt ccgcctgcaa gcccgggctg atcgtcagca
     2941 cgcaacccgc gcagacgatg gcgtaaaaag atgatttcaa tggattctcc tcgagtccct
     3001 cagtctgtcg ggatcggtcc cgacgcacgg acccggtagt caggcgcgcg cgcggctgac
     3061 cgggagtgca aatgctgcgt ggaatcaggc tcggggtggt ttgtacagcc gagtttggta
     3121 ctgtggtacc gggtcggtgc cggtcagggg aaagcgggcg gtgctcgaca tccgctcgga
     3181 cggggccgcg aaaatgccgg gaataaagcc ggtgcactgc gcgcagagcg agtcgcagcc
     3241 gagcgtgtgc tcgtcatgat ggccgcccat ctccccggtc atgtggcagg gcgtggagtg
     3301 ctccccaggt tgggagagca gggtcgactg cagctccatc gcgccgatcg ccgcggtgtt
     3361 cagcaacagt atcgcggcca gcatcagccg agaaactctg cgggcgactt tcgatccgta
     3421 aggtgccatt ttctgcgcgt tcactccagc gtcggttctt tactcgggat ttgagttcgc
     3481 cgaaccatat agttttgtta tacgacaaga tcaagtcggt ggatcaccgt cgacacgagc
     3541 cacgaaatcc cgggcgaacc cgccgtctgg agtgatcgta actgaaactg gctcaaaatg
     3601 gcaatagcgt taccggggaa ctggggacca atatcagtat ctggggtcgg gtgataggta
     3661 atcgggtaaa gtttgagtat atgaatcgcg gtgtcatcgg tgaaggtgag ttgttgttcc
     3721 atgccctgcg taacgcggct tcgagtatct ggaagtcagg ttcgaccaat gccagaactt
     3781 caaatactcc caacctctgg aatctggctc gaatcgaata aaactcagag ggaaattcgt
     3841 ctactggcag gcgtcggtaa tgcgagagtt caatgaaatt tgatcaccgt caagtgtctg
     3901 gatcgaattt cgatactccc cggcaagcac ggtgcttcaa ttctgcgatc gtttttgttc
     3961 gtctacggcc tattttaatt taccaatgaa aaacgcaagc agtcggatta gagtgctaga
     4021 ttcttattgt cgatgaaggt ctgctacggg tccagactaa tggaatggtc gcccacctaa
     4081 attatttata cgtcagcttt cccaataaca gacgcttaca gacgaaggcc aggagacgac
     4141 cttcggccaa tagcggaagt tgccaagctc tacctttgcc ctaggaatca cggcgcc
//
LOCUS       DATCZV010000388         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_774143, whole genome shotgun sequence.
ACCESSION   DATCZV010000388 DATCZV010000000
VERSION     DATCZV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774143"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1246)
                     /locus_tag="VIW27_08765"
     CDS             complement(62..1246)
                     /locus_tag="VIW27_08765"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5739803.1"
                     /translation="MMSENIVITGIARTPMGGFQGVLSDASAAQLGATAIRAAVERSQ
                     LQGDDVDEVIMGCVLPAGQGQAPARQASLGAGLPLATGCTTINKMCGSGMKATMFAHD
                     MLRAGNNRVMVAGGLESMTNAPYLLTKARAGFRMGHGQVYDHMFLDGLEDAYDKGKLM
                     GVFAEATADKYGFTREQQDEFAITSLKRAQAAIESGAFADEIAPVVLKTRKGEVTVDT
                     DEQPFNANLEKIPGLRPAFRQDGTVTAANSSSISDGSAALVLMSESEAGRRGVKPLAA
                     IRAHATHAHEPAWFTTAPVGALKKVLEAANWSSDEVDLYEINEAFAVVTMAAMTDLGL
                     DHAKVNVNGGACALGHPIGASGARIIVTLISALQRQGLKKGVAGLCIGSGEATAVAVE
                     LV"
     gene            complement(1254..2417)
                     /locus_tag="VIW27_08770"
     CDS             complement(1254..2417)
                     /locus_tag="VIW27_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY5739804.1"
                     /translation="MIHIPSLNFDLGETVDMLRDAVQDFAQAEIAPLAAEIDRSNEFP
                     NQLWPKLGDMGLLGITVPESYGGSAMGYLAHIVAMEEISRASASVGLSYGAHSNLCVN
                     QINRNGNEQQKQKYLPKLVSGEHIGALAMSEPGAGSDVVSMQMKAEKTSAGYLLNGTK
                     MWITNGPDADLIVVYAKTDPAAGSRGISAFLVEREFGFTTAQKLDKLGMRGSNTGELV
                     FQDVEVPAENLLGELNRGAAVLMSGLDFERAVLSAGPTGIMLACMDAVLPYIHERRQF
                     GQPIGEFELMQGKIADMYTTMNATRAYVYAVGKACDRGETTRKDAAGAILYAAEKATW
                     MALEAIQALGGNGYTNDFPVGRLLRDAKLYEIGAGTSEIRRMLIGRELFNETS"
BASE COUNT          412 a          818 c          790 g          517 t
ORIGIN      
        1 gtagtcgcgc acgctgtcgc gaatcaggcg ctgttcttcg ttgaggttca tcggccaggt
       61 atcaaaccag ctcgaccgcc accgcggtcg cttcaccgct gccgatgcag agcccggcga
      121 cgcccttctt caggccctgg cgctgcagtg ccgaaatcaa cgtaacgatg atacgcgcac
      181 cggacgcgcc gatcggatgg ccgagcgcac aggcgccgcc attgacgttg actttcgcgt
      241 gatcgagccc gagatcggtc atcgccgcca tcgtcaccac cgcgaatgcc tcgttgatct
      301 cgtacagatc gacctcgtcg ctggaccagt tggccgcttc gagcaccttt tttaacgcac
      361 ccaccggggc ggtggtgaac cacgcgggct cgtgcgcgtg ggtggcatgc gcgcggattg
      421 cggccaacgg cttcacgccg cgtctgccgg cttcggactc gctcatcaat accagcgccg
      481 cgctgccgtc ggaaatcgaa ctcgaattgg ccgcggtcac ggtgccgtcc tggcgaaacg
      541 ccggacgcaa gccggggatt ttttcaaggt tggcgttgaa tggctgctcg tcggtatcga
      601 ccgtgacttc gcccttgcgc gtcttgagca cgaccggcgc gatttcatcg gcgaaggcgc
      661 cgctttcgat cgcagcctgg gcgcgcttca gcgaagtgat ggcaaactcg tcctgctgct
      721 cgcgggtaaa gccgtacttg tcggcggtgg cctcggcgaa cacgcccatc agcttgccct
      781 tgtcgtaggc gtcctcgagc ccgtcgagaa acatgtgatc gtagacctgg ccatggccca
      841 tgcggaaacc ggcgcgcgcc ttcgtcagca ggtagggagc attggtcatg ctttcgaggc
      901 cgcccgccac catgacccgg ttgttaccgg ctcgtagcat atcgtgggcg aacatcgtgg
      961 ctttcatgcc cgagccgcac atcttgttga tcgtggtgca cccggtggcg agcggcaatc
     1021 cggcgcccag gctcgcctgg cgcgccggcg cctggccctg gccggccggc agcacgcaac
     1081 ccatgatgac ttcatcgacg tcgtcgccct gcaactggct gcgttcgacc gcggcccgga
     1141 tcgcggtggc gccgagctgc gccgcgctgg catcggacaa tacgccctgg aagccaccca
     1201 tcggggtgcg ggcgataccg gtaatgacga tattttcact catgattgtt accctaggaa
     1261 gtctcgttaa acagttcccg cccgatcagc atgcggcgga tttcggaagt gccggctccg
     1321 atttcgtaga gcttggcatc gcgtaacagg cggcccaccg ggaaatcgtt ggtgtagccg
     1381 ttgccgccca gcgcctggat cgcctcgagc gccatccagg tcgccttttc ggcggcataa
     1441 agaatcgccc cggcggcgtc cttgcgcgtg gtttcgccac ggtcgcaggc cttgccgacg
     1501 gcatagacat aggcccgggt cgcgttcatc gtggtgtaca tgtcggcgat cttgccctgc
     1561 atcagttcga attcgccgat cggctggccg aactgcctgc gttcatggat atagggcagg
     1621 accgcatcca tgcaggccag catgatgccg gtgggcccgg ccgacagtac cgcgcgttcg
     1681 aaatcgagtc cgctcatcag gaccgcggcg ccgcggttga gttcgcccaa caggttttcc
     1741 gccggcacct cgacgtcctg gaacaccagc tcgccggtgt tggaaccgcg catgccgagc
     1801 ttgtcgagct tttgcgcggt ggtgaatccg aactcccgct ccaccaggaa ggcgctgatg
     1861 ccgcgcgacc ctgccgccgg gtcggtcttg gcgtaaacca cgatcaggtc tgcatcgggg
     1921 ccgttggtaa tccacatctt ggtgccgttc aggagatagc ccgccgaggt cttttcggcc
     1981 ttcatctgca tgctgaccac gtccgagccg gcgccgggtt cggacatcgc cagcgcgccg
     2041 atatgctcgc cgctgaccag tttaggcagg tatttctgct tctgctgttc gttgccgttg
     2101 cggttgatct ggttgacgca caggttcgaa tgcgcgccgt aactcaggcc gaccgaggcc
     2161 gaggcgcggc tgatttcctc catcgcgacg atatgcgcga ggtatcccat ggcgctgccg
     2221 ccgtagcttt ccggcacggt aatgccaagc aggcccatgt cgccgagttt cggccacagc
     2281 tggttgggaa attcgttgct gcgatcgatt tcagccgcca gcggcgcgat ctcggcctgc
     2341 gcaaaatcct gcaccgcgtc gcgcagcatg tcgacggttt caccgaggtc gaagttcagg
     2401 gacgggatat gtatcatcag gtttctcccg gttttaaccg tggctcagct tgcgttggct
     2461 ggaacgaatt tcctgcagtg ccgcctcggc attggtggca agacgctcca ggtcagccag
     2521 cgcattatcg atatccc
//
LOCUS       DATCZV010000389         4517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_455804, whole genome shotgun sequence.
ACCESSION   DATCZV010000389 DATCZV010000000
VERSION     DATCZV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455804"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1073
                     /locus_tag="VIW27_08775"
     CDS             396..1073
                     /locus_tag="VIW27_08775"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5739805.1"
                     /translation="MSVNLAMQQLTDNDVYWWRPLDDTEPDTREQILFAAYKEFHLNG
                     FQSASLSHILARTGVTKGALYHHFPNKTALGYAVVDEVIARRIQLSFIEPLEHFDDPI
                     EGFIELVRGAGNTFSMTDIQLGCPLSVLAQEMAPIDEGFRIRLTAIYEQWHSAIAAVL
                     SRAQAEGRIIESVDTEIMAATLMSIMEGSLSAAKVTQSLDTLHACGAGLVQVLQLIRS
                     PAERVNG"
     gene            1066..3417
                     /locus_tag="VIW27_08780"
     CDS             1066..3417
                     /locus_tag="VIW27_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5739806.1"
                     /translation="MAERYARFVLRHKWAAILLALLWIAALGFGARHLTFTTDYRVFF
                     GDDNPQLQAFENLQDTYSRNDNVLFVLAPRDGEVFTREALGAIAWLTEQAWQMPYSTR
                     VESLTNYQHTSADGDDLLVADLAWEAESLSDAELRRIRGIALAEPVLVNRLVSPRAHV
                     AGINVTIELPGVDVNAENPEVVTFARQLHDRFEARYPSIDARLTGIVIMNQAFPEASQ
                     YDMTRLFPLMLVLFMAVLFFWVKGLAGTLATFLVIVFSIAGAMGTAGWLGIALTPPSF
                     SAIMIIPTMAIADSVHVLMNYVLTRQQGGGREEAMVDSLRINLQPVFLTSVTTAIGFL
                     SMNFSDAPPFHDLGNIVAIGVMIAFVLSVSFLPALTMLLPGRQRIAQSASSSLMLRFA
                     EFVIARRRRLLIGMGLGSLLLVSFVPQNELNDDFVKYFSERIEFRRDADFASEHLAGL
                     YLIDYSLESGKDGGVSDPAFQHQAERFANWYRQQPEVAHVNVITDTFKRLNRSMHGDD
                     EAWYRLPQARDLAAQYLLLYEMSLPYGLDLNNQINIDKSATRMTVLLENMTTKTVLAL
                     EQRAQAWLRDNASTGLKTTGASPTIMFSHIGERNIRSMLLGTTIALVLISLILVVALK
                     SPRIGLVSLVPNLIPAAMAFGVWGIAVGQVGLALSVVTGMTLGIVVDDTVHFLSKYLR
                     ARREKGLAAEDAVRYAFGTVGLALVVTSIVLVAGFLVLAFSAFTLNSNMALMTSATIL
                     FALIADFLLLPPLLMAIDGGSHPPAAARQAQLSDPAGKEMTDA"
     gene            3410..4195
                     /locus_tag="VIW27_08785"
     CDS             3410..4195
                     /locus_tag="VIW27_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY5739807.1"
                     /translation="MPRTRLAILAALLAACTAAAAQSAEQKGLQIVRAADAYDRGFGD
                     FTANMVMTLKNKSGDTSRRLIRIRALEVDGDGDKSMSIFDEPADVKGTKMLTYSHGLE
                     PDDQWLYLPALKRVKRISSRNKSGPFMGSTFAFEDLGSQEVEKYTYRYLRDEACGEYQ
                     CHVIEFYPAYEFSGYTRQVVWMDTEAYRVVRVDYYDRKQALLKTLTAADFQQYLGHYW
                     RPGRMEMLNHQTGKSTLLEWTDYRFGTGLDDSDFRPQTLKRSD"
     gene            4203..>4517
                     /locus_tag="VIW27_08790"
     CDS             4203..>4517
                     /locus_tag="VIW27_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739808.1"
                     /translation="MKRILLTLLLSLAATTAVAEFSGNVALEAQAFNTPARFEEQFDN
                     NATMSFQPRWDGEWNDGDDLWSVELFMRADNKDDGRQHADLREALWLHIAGDNEWRLG
                     VNT"
BASE COUNT          831 a         1461 c         1371 g          854 t
ORIGIN      
        1 agtccgtcga gacgaaacgt cagaaccgtc accccggccc ccgagccggg gtccatcgcg
       61 acgcgactcc agaaccgtca ccccggcccc cgagccgggg tccatcgcga cgcgacgcca
      121 caaccgtcac cccggccccc gagccggggt ccatcgcggc tgcgctgccg ggctatgcat
      181 tgatttcgag tttcgaatga acttttcagc tggtcgatag tcaatcgacc ggaatggaga
      241 ccggcggtcc ggcgtcccgg tcgagcctgg catggcattg caccgatctt cgctttgctg
      301 gccgctgttc acgtggacgg ttgtcgacct gcgagccgaa aaagttgaca ttcataccgg
      361 tcggtatgta gactacacct tccataccga ttggtatgtc cgtgaacctg gcgatgcaac
      421 aactgaccga caacgacgtt tactggtggc gcccgctgga cgacaccgaa cccgatactc
      481 gcgagcagat cctgttcgcg gcctacaagg aatttcatct caatggcttc cagtccgcca
      541 gcctgagcca tatcctggcg cgcaccgggg tcaccaaggg cgccctgtac caccattttc
      601 cgaacaagac cgcgctcggt tacgcggtcg tcgacgaggt catcgcacgc cgcatacagc
      661 tcagtttcat cgagccgctc gagcacttcg acgatccgat cgagggcttc atcgaactgg
      721 tcaggggcgc cggtaacact ttctcgatga ccgatatcca gctcggctgc ccgttgtcgg
      781 tgctggccca ggaaatggcg ccgatcgacg agggctttcg tatccggctg acagcaatat
      841 acgaacagtg gcactcggcg attgccgcgg tgctgtcgcg cgcgcaagcc gagggacgga
      901 tcatcgagtc ggtcgatacc gagatcatgg cggcgacgct gatgtcgatc atggaaggca
      961 gcctgagcgc ggccaaggtc acgcaaagcc tcgacacgct gcacgcctgc ggtgccggtc
     1021 tggtccaggt gctgcaattg attcgcagtc cggcggagcg ggtcaatggc tgagcgttac
     1081 gcgcgtttcg tcctgcgcca caagtgggcc gcgatactgc ttgcgctgct gtggatcgcg
     1141 gcgctcggct tcggtgcccg ccacctgact ttcaccaccg actaccgggt gtttttcggc
     1201 gacgataacc cgcagttgca ggccttcgaa aacctgcagg atacctattc gcgcaacgat
     1261 aacgtgctgt tcgtgctggc accgcgggat ggcgaggtgt ttacccgcga agccctcggt
     1321 gcgatcgcct ggctgaccga gcaggcctgg cagatgccct actcgacgcg cgtcgaatcg
     1381 ctgaccaatt accagcatac ctccgccgac ggcgatgatt tgctggtcgc ggacctcgcc
     1441 tgggaagccg aatcgctgag cgacgccgag ctgcggcgga ttcgcggcat cgcgctcgcc
     1501 gagccggtgc tggtcaaccg cctggtatcg ccgcgggcac atgtcgccgg gatcaatgtc
     1561 accatcgagt tacccggtgt cgacgtgaat gccgaaaatc ccgaagtcgt caccttcgcg
     1621 cgccagttgc acgatcgctt cgaggccagg tatccgtcta tcgacgcgcg cctgacgggg
     1681 atcgtaatca tgaaccaggc cttccccgag gccagccagt acgacatgac gaggctgttt
     1741 ccgctgatgc tggtgctgtt catggcggtg ttgttcttct gggtcaaggg tctggcgggt
     1801 acgctggcga ctttcctggt gatcgtgttt tcgatcgccg gcgcgatggg cacggccggc
     1861 tggctcggta tcgcgctgac cccgccgtcg ttttcggcga tcatgatcat accgaccatg
     1921 gcgatcgccg acagcgtgca cgtgctgatg aactacgtgt tgacgcgtca acagggcggc
     1981 ggccgcgagg aagcgatggt cgacagcctg cgtatcaacc tgcagccggt attcctgacc
     2041 agcgtcacca ccgcgatcgg tttcctgagc atgaattttt ccgacgcgcc gccgttccat
     2101 gatctcggca acatcgtcgc catcggcgtg atgatcgcgt tcgtgctcag cgtaagcttt
     2161 ctaccggcgc tgacgatgct gctgccgggc cggcagcgga tcgcgcaatc cgcttccagc
     2221 agcctgatgc tgcgtttcgc cgagttcgtc atcgcgcgcc gccggcggtt gctgatcggc
     2281 atggggctgg gcagcctgct gctggtttcg ttcgtgccgc aaaacgagct caacgacgat
     2341 ttcgtcaagt atttcagtga acgcatcgag tttcgccgcg acgccgattt cgcctccgag
     2401 cacctcgccg ggctttacct gatcgattat tcgctcgaat cgggcaaaga cgggggggtc
     2461 agcgatcccg cctttcagca ccaggcggag cgcttcgcga actggtaccg gcaacagccg
     2521 gaggtggccc acgtcaacgt gatcaccgat accttcaaac gactcaaccg cagcatgcac
     2581 ggcgacgacg aagcctggta ccggctgccg caggcgcgcg atctggccgc gcagtacctg
     2641 ctgctgtacg aaatgtcgct gccctatggc ctcgatctga acaaccagat caacatcgac
     2701 aagtcggcaa cccgcatgac cgtgctgctc gaaaacatga ccaccaaaac ggtgctcgcc
     2761 ctcgagcagc gcgcgcaagc ctggctgcgc gacaatgcca gcaccggcct gaagaccacc
     2821 ggcgccagcc cgacgatcat gttcagtcat atcggcgagc gcaatattcg cagcatgctg
     2881 ctgggcacca cgatcgccct ggtgctgata tcgctgattc tggtcgtggc attgaagtcg
     2941 cccaggatcg gcctcgtcag cctggtgccg aacctgatcc cggcggcgat ggcattcggc
     3001 gtctggggca tcgcggtcgg ccaggtgggg ctggcgctgt cggtggtgac cggcatgacg
     3061 ctcggcatcg tcgtcgacga taccgtgcat tttctcagca agtacctgcg cgcgcgccgc
     3121 gagaagggcc tggctgccga agacgcggta cgttacgcat ttggcaccgt cggcctggcg
     3181 ctggtggtga cctcgatcgt cctcgttgcg ggcttcctgg tgctggcttt ttcagccttc
     3241 acgctcaact cgaacatggc cctgatgacc tcggcgacta tcctgttcgc gctgatcgcc
     3301 gattttctac tattgccgcc actgttgatg gctatcgatg gcggcagcca cccgccggcc
     3361 gcggctcgcc aggcgcaact atccgaccct gccggcaagg agatgaccga tgcctagaac
     3421 aagactcgca atcctcgcgg cgttgctggc ggcctgcacc gcggccgccg cacagtccgc
     3481 cgaacaaaaa ggcctgcaga tcgtgcgcgc ggcggacgcc tatgaccgcg gctttggcga
     3541 cttcaccgcc aacatggtga tgacgctgaa gaacaaatcc ggcgatactt ccaggcgcct
     3601 gatccgcatt cgtgcgctcg aggtcgatgg cgacggcgac aaaagcatgt cgatcttcga
     3661 cgaacccgcc gacgtcaagg gtaccaagat gctgacctat tcccacggtc tcgagcccga
     3721 cgaccagtgg ctttacctgc cggcgttgaa gcgggtcaag cgtatcagct cgcgcaacaa
     3781 gtccggcccg ttcatgggtt cgaccttcgc gttcgaggat ctcggctccc aggaagtcga
     3841 aaaatacacc taccggtacc tgcgcgacga agcctgcggc gaataccagt gccatgtgat
     3901 cgagttctac ccggcctacg agttttccgg atatacccgc caggtcgtgt ggatggatac
     3961 cgaggcatac cgcgtggtcc gggtcgacta ctacgaccgc aagcaggcgt tgctgaagac
     4021 cctgaccgcc gctgattttc agcagtatct cggccattac tggcgcccgg gccgcatgga
     4081 aatgctgaat caccagaccg gcaagagtac gctgctcgaa tggaccgact accgcttcgg
     4141 aaccggtctc gacgacagcg atttccggcc gcagacgctc aagcgaagcg actgaggcgg
     4201 cgatgaaacg catcctgcta accctcctgc tgtcgctggc cgcgaccacc gcggtcgccg
     4261 agttctccgg caacgtcgcg ctcgaagcgc aggccttcaa cacgccggcg cgcttcgagg
     4321 agcaattcga caacaatgcc accatgtctt tccagccgcg ctgggacggc gagtggaacg
     4381 acggcgacga cctgtggagc gtcgagctgt tcatgcgcgc cgacaacaag gacgacggcc
     4441 gccagcacgc cgacctgcgc gaggcgctgt ggctgcacat cgccggcgac aacgagtggc
     4501 gcctcggcgt taacacc
//
LOCUS       DATCZV010000390         2371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_508758, whole genome shotgun sequence.
ACCESSION   DATCZV010000390 DATCZV010000000
VERSION     DATCZV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508758"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            702..1925
                     /locus_tag="VIW27_08795"
     CDS             702..1925
                     /locus_tag="VIW27_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5739809.1"
                     /translation="MLSIQEVKNAKAGDKPRKFYDRDGLYLFVTPAGGKYWRGKDRVN
                     GREKTLSLGTYPKFGLAEARDMWQDARKLDDPSAVKQAEKHALDSSTPTFLAVTRAWH
                     TRQTPAWTRKHASQVWRSLEIDILPTLGARPIDEIQPREIMALIEGIEDRGAGEIATR
                     VLQRVRAVFSRAVALGYREVNPASELRNHLKPRKKGQQTALAADELPAFLHALDAYNG
                     DTATRLGLRLLILTLARTSEVREAKWAEFDREKGVWTVPAERMKNRREHRVPLSQQAL
                     ATLEELHKVTGHSQWLMPGRLEDKPASQNTMIFAVYRMGFHKRTTVHGFRALASTILN
                     EAVTEIDGKKHRMWSVDAVERALAHTEQNKVKGAYDRGDRFEERTRLLQWWADYLDAV
                     AKSSDTAVAAIAAEV"
BASE COUNT          533 a          733 c          699 g          406 t
ORIGIN      
        1 cactccgccg ttccaagcga ctaaaatcgt ggcttttttc gacctggagc gacccaaaac
       61 gccaacttgc cgaccacgtc gcccaaagct atgtggccgg tgcgcacgag gtcatggctg
      121 gacgagcact cacgatttcc cgaccgagcg actttgcctc ctggcctact cgccgtaccc
      181 gcgagccgga tggacccagc agtggaaata gacgcctacc caggacctgt cgggccgcat
      241 cccggccatg gttcgggagc tggagaaagc caccgtcgaa atcgcacggc tcgtcgagat
      301 agaacggctt cgacaggcgc gcgccctggt cggcggcact gatgcgttgg agcgattcga
      361 cgcatggcga gcgccggagg agcgataaaa tcggtttgat ttcaggcagt taatcagccg
      421 aatcgatttg ggggtatctg tgggggcata atcggctttt agcattttaa gccattgatt
      481 tataaggcaa tgtgattcct gccgactcca ccattaccga gttttcaagc ccctgaaaac
      541 tcaagagaaa gcgcccccag tggcgctttt ttcttatcta aatcaagcaa atacgtttag
      601 caaaagcata tctcgggata tctggtaatc tccggccttc acagctttac tgggggtatt
      661 tttgggggta taaaaaaagc cccaacagaa aataccccca gatgctcagc atacaggaag
      721 tgaagaacgc caaggccggc gacaagccgc ggaagttcta cgatcgggac gggctgtacc
      781 tcttcgtcac ccccgccggc ggcaagtact ggcgcggcaa agaccgcgtc aatgggcggg
      841 agaagaccct gagcctcggc acctatccca agttcggcct cgccgaagcc cgcgacatgt
      901 ggcaggacgc ccgcaagctc gacgacccct ccgcggtaaa gcaggcagag aagcacgctc
      961 tcgattcgtc gacgccgacc ttcctcgcag ttaccagggc ttggcacaca cgtcagacac
     1021 ccgcctggac gcgcaagcac gcttcacagg tttggcggtc cctggagatc gacatcctgc
     1081 cgacgttagg cgcgcgccct atcgacgaga tccagccgcg cgagatcatg gcgttaatcg
     1141 agggaatcga ggaccgcggc gccggcgaga tcgcgacccg cgtgttgcag cgtgtacgag
     1201 ccgtgttctc gcgtgccgtc gcgctcggct accgtgaggt caacccggcc agcgagctcc
     1261 gcaatcacct caagccgcgc aagaagggac aacagaccgc gctcgctgcc gacgaactcc
     1321 cggcgttcct gcacgcactc gatgcctata acggcgacac ggctacgcgc ctgggtctgc
     1381 gcctgctgat cctcaccctc gcccgcacaa gcgaggtccg cgaagcaaag tgggccgagt
     1441 tcgaccggga gaaaggagtg tggacggttc cggccgagcg gatgaaaaac cgccgcgaac
     1501 accgcgtgcc cctgtcgcag caggcgctcg ccacgctcga ggagctgcac aaggtcaccg
     1561 gccattccca gtggctgatg ccaggccgcc tcgaggacaa gccagccagt cagaacacca
     1621 tgatcttcgc cgtctaccgg atgggcttcc acaagcgcac caccgtccac ggcttccgag
     1681 ccctggcctc gacgatcctc aacgaggccg tcacggagat cgacggcaag aagcatcgca
     1741 tgtggtccgt cgatgcggtc gaacgcgctc tcgcccacac cgagcagaac aaggtcaagg
     1801 gtgcctacga ccggggtgac cgattcgagg agcggacccg cctgttgcag tggtgggcag
     1861 attacttgga cgccgttgcg aaatcgtcgg acacggcggt agctgcgatc gcggccgaag
     1921 tgtgaagagc ttccccggcg atgtcaggca cttagaaacc acgacgctgg ttgcattggt
     1981 tccctgcagt catcgccagc gatatcacta ccatccatat ccgagaggtc gcgatagaac
     2041 aggagaccga tggtgaaacg actcattact gacaagcaac tgaaggaaaa gctcggcgga
     2101 tgctccgaca tgacaatctg gcggctgcgc cgggatggca agctaccgaa acccaggaag
     2161 ctcgggagtc gaaacgttac gcccgaggac gaggccgata aggcgatcgc tacgctaatt
     2221 gggttgtgat ctgagaccga acgatgggaa cgtcggcgca gccgcgtctt tcgcaacaat
     2281 ggccgtcaga tcatcgccga agatgtcgca gccaatccta cccggtagca acgagcaaat
     2341 ccgatggtct ggactgatgc atccgacgat c
//
LOCUS       DATCZV010000391         5426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_563302, whole genome shotgun sequence.
ACCESSION   DATCZV010000391 DATCZV010000000
VERSION     DATCZV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563302"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..245
                     /locus_tag="VIW27_08800"
     CDS             30..245
                     /locus_tag="VIW27_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY5739810.1"
                     /translation="MYGWHNFGGGFMWLVWILIIVALVWFVMIATRERGDDSQRNPSA
                     LEILRQRYARGEIDRQEFEQKKQDLDG"
     gene            481..936
                     /locus_tag="VIW27_08805"
     CDS             481..936
                     /locus_tag="VIW27_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739811.1"
                     /translation="MLKPRYLATMIFAALVAAPVWAAEDSPPDASATMPMMQQGGNAM
                     TPAAMMQMMQQGSGMMSPGAMMPMMQQGGGMMAPGYGQAGGHHGMHPMVGGMGHMMMY
                     PQMMGGGMGNMMMHPQMMQMHMQHMHTMQQHLKNIESLLQELVDQGKSG"
     gene            1145..1783
                     /locus_tag="VIW27_08810"
     CDS             1145..1783
                     /locus_tag="VIW27_08810"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEY5739812.1"
                     /translation="MGSLGPLLVLGLMVLAIVFNPLPSAPIAIAAGAVYGHTLGTVYI
                     VAGAEIGAIIAFFIARTIGYEFVSRHFGKFDRLHRFGTQNGLMLMVFVARLLPFMSFD
                     LVSYAAGLTPLTAWRFALATLFGLLPMSFLLAHMGSEIVDGEVEDLAATVLLLGLLVT
                     VPLIAGQFRRGRDAEDASRTHRNVDGEQENSASPQSTTGKSATRSILTRKRD"
     gene            1789..2601
                     /locus_tag="VIW27_08815"
     CDS             1789..2601
                     /locus_tag="VIW27_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5739813.1"
                     /translation="MYKVKLFLLFSLALAAFGLQGAEIPTPELPKVNGFAIVDDEGEQ
                     YIAATNQGLYHSHDHGHSWRAHKTGFGLPATMVATTPDGSVYAFVVTLGLLKLDGKTN
                     EWQTINNRFGSQVLQQLSATRWTPSRLVARNHYGKLIASENYGDDWYKLRGPYHPQTD
                     AEKRGHDIYIEKCQSCHGVDGVGETYSRQALTDKNYFMAPALDESAHAWHHTDQQLAE
                     LILEGSSRTQRMAAWKNADVSDADARDLVAYIKSLWTQRELDCQGPKHMECM"
     gene            complement(2718..2978)
                     /locus_tag="VIW27_08820"
     CDS             complement(2718..2978)
                     /locus_tag="VIW27_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739814.1"
                     /translation="MDRLRASQIHADDRTRIIAIESVHCRPGKFAGVYHLYAGIEPVA
                     LIVCTAGGNRLVSLTMADTDLDELQREIRGLRALLAWDQSNG"
     gene            complement(2997..3581)
                     /locus_tag="VIW27_08825"
     CDS             complement(2997..3581)
                     /locus_tag="VIW27_08825"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HEY5739815.1"
                     /translation="MWSAIVFTVLVLIALLSIPLSLTYQFSWRQRLRGEASLQWLFGL
                     VRVRLAPTRKPAANSRRAGGAGGPTPKPARSKLDPLAALRQKAFRRRMLRFARQFWCA
                     FRKRDLHLRIRIGLGDPADTGRIWALFGPLAGLLANVEGASIELQAEFADAVFEVDSG
                     GRVSVIPLQLFGLLLGLLLSPPFWRGMRGMRRTA"
     gene            complement(3597..3953)
                     /locus_tag="VIW27_08830"
     CDS             complement(3597..3953)
                     /locus_tag="VIW27_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEY5739816.1"
                     /translation="MQDIEKLFDKAVTEIERMLNTKTVVGEPITAGDATLIPLVNVGF
                     GFGVGGGKGSEPNKGSGEGGGTGGGGGVKPVAVVIITADGVRVEQLKSSTASVLEKVA
                     ESIGKVAAAKGSKQSD"
     gene            4465..4803
                     /locus_tag="VIW27_08835"
     CDS             4465..4803
                     /locus_tag="VIW27_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY5739817.1"
                     /translation="MNDTLRQGTQPVSDGDQGIAIQSFYPDHKGQLTRLARIEGQIKG
                     ITRMIEERRYCVDIVSQIKAVQAALKQVQMGVLEKHIHHCVAGAANSGDAAQLNEKIE
                     EIVQVVAKLD"
     gene            4818..>5426
                     /locus_tag="VIW27_08840"
     CDS             4818..>5426
                     /locus_tag="VIW27_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HEY5739818.1"
                     /translation="MVASKSRLASEIGAEAAASPTHGALQRETLRVTGMTCGACSARV
                     ERVVGKLDGVTAASVNLATERMDVQFDTARIDLPQIRAAVEKAGYGIEEAEQAREILL
                     PVEGMTCAACAARIEKVVGSLDGVRSIVVNLLADNARIRYLPAVTSLAAIREAIEATG
                     YRTYEVVESSGSDDRQMQHDDALRRQRRDLLVALAFTLPLLAM"
BASE COUNT         1100 a         1691 c         1656 g          979 t
ORIGIN      
        1 aaaacctatg gttacagcga ggtgacacca tgtatggctg gcataatttc ggcggaggat
       61 tcatgtggtt agtctggatt ctgatcatcg tcgccctggt ctggttcgtg atgatcgcga
      121 cccgggagcg cggggacgat agccaacgga atccatcggc gctcgagatt ttacggcaac
      181 gttacgctcg cggcgaaatc gatcgccagg aattcgagca aaagaaacag gacctggacg
      241 gatgaccgtt cgcctatcgc gacccgttac cgtttataca aaccggtccg cagcaatcgc
      301 aatcgccgct cgcgatcaac catgtctcgg caggatagcg ccatgcgcgc aggtttcccc
      361 aggcgcccgc agccgatcgt gcgggcgcgg atttttcgaa ccttcgcaaa caattgctgg
      421 atacggaccg cctgctcgcg acagctttcg gactcactca acccacgaga ggaaatacag
      481 atgttaaagc caagatacct tgcgaccatg atttttgccg ccctggtggc ggcgccggtg
      541 tgggcggccg aggattcgcc tcccgatgcc tccgctacaa tgccgatgat gcaacagggc
      601 ggcaacgcga tgacgcccgc ggccatgatg cagatgatgc agcaaggcag cggcatgatg
      661 tcgcccggcg cgatgatgcc gatgatgcag cagggcggcg gcatgatggc gcccggttac
      721 ggccaggccg gcggccacca tggtatgcac ccgatggtcg gcggcatggg ccacatgatg
      781 atgtatccac agatgatggg gggcggtatg ggcaatatga tgatgcatcc gcagatgatg
      841 cagatgcaca tgcagcatat gcataccatg cagcagcacc tgaaaaacat cgagtcgctg
      901 ttgcaagaac tcgtcgacca gggcaaatcg ggctaggcgc gcgcggcccc ggtaccagtg
      961 gtatcggggc cgacgcgcca ggttcacgcg gcgagggcag cgatccatgg cggaatttcg
     1021 gctcaggtat attttgataa cggcattgct ggtcgcactg ccggcgatct atttcgccct
     1081 gcaaaattcc ggcctgatgg tttatttcac cgcccccgga ccgatcgtcg aacaggtacg
     1141 tgcgatgggc agcctcggtc ctctcctcgt gctcgggcta atggtgctgg ccatcgtatt
     1201 caatccccta cccagcgcac cgatcgcgat tgcggccggc gcggtatacg ggcacacgct
     1261 gggaacggta tacatcgtcg ccggcgccga gatcggcgcc atcattgcgt ttttcattgc
     1321 ccgcacgatc ggctacgagt tcgtcagccg gcacttcggc aaattcgacc ggctgcatcg
     1381 cttcggcacc cagaacggcc tgatgctaat ggtcttcgtg gctcgcctgc tgccattcat
     1441 gtcgttcgac ctggtcagct atgcggccgg cttgacccca ctgaccgcgt ggcgctttgc
     1501 gcttgccacg ctatttgggc tgctgcccat gagcttcctg ctcgcccaca tgggcagcga
     1561 aatcgtcgac ggtgaagtcg aagaccttgc cgcgaccgta ctgctgctgg gtctgctggt
     1621 cacggtaccg cttatcgccg ggcaattccg ccgcggacgc gatgcggaag acgcctcccg
     1681 gacccaccgc aatgtcgacg gtgagcagga gaattcagca agcccgcaat cgacaacggg
     1741 caaatcggcc actcgatcaa tccttactcg aaaacgagac tgacatccat gtacaaggtt
     1801 aaattatttt tgcttttttc gctggcgctg gcggcattcg gcctgcaggg cgcggaaata
     1861 cctaccccgg aactgcccaa ggtcaacggc ttcgcgatcg tcgacgatga gggcgaacaa
     1921 tatatcgcgg cgaccaacca ggggctctat cacagccacg atcacggtca tagctggcgc
     1981 gcgcacaaga ccggtttcgg tctgccggcg accatggtgg cgacgacacc cgatggctcg
     2041 gtctacgctt tcgtcgtgac cctcggactg ctcaagctcg acggcaaaac caatgaatgg
     2101 cagacgatca acaaccgctt cggcagccag gttctgcaac aactgagcgc tacccgctgg
     2161 accccgtcgc ggctggttgc gcgcaatcat tacggcaagc tgatcgcatc cgaaaactac
     2221 ggtgacgact ggtacaagct gcgcggcccc taccatccgc aaacagacgc cgaaaagcgg
     2281 ggacatgaca tctatatcga aaagtgccag tcctgccacg gcgtcgacgg cgtcggcgaa
     2341 acctattcac ggcaagcgct gaccgacaag aattatttca tggctcccgc gctggacgag
     2401 tcggcccatg cctggcatca caccgatcag cagctggccg aactgattct cgaaggctcg
     2461 tccagaaccc agcgcatggc cgcgtggaaa aacgccgacg tctccgacgc ggatgcgcgc
     2521 gacctggtcg cctacatcaa gagcctgtgg actcagcggg aactcgactg ccaggggccg
     2581 aaacatatgg aatgcatgta gcgagcgact ccctcgcggc aagcaggtaa accgaccgtg
     2641 gcaacaggca tcgagccacg ggaaaaggac tcgggtctcg ccttcgagca gccgttcgaa
     2701 cgcgcggcag cgcagcgtta gccgttactc tggtcccacg ccagcagcgc gcgcagtccg
     2761 cggatctcac gttgcaattc gtcgaggtcg gtatccgcca tcgtcaggct gaccagccgg
     2821 ttgccgccag cggtacagac gatcagcgca acgggttcga tacccgcgta caggtgatac
     2881 acgccagcga atttgccagg cctgcaatga accgattcga tggcgattat ccgggtcctg
     2941 tcgtcggcat gtatctggct ggcgcgcaac ctatccatcc ctaatccccg cggcagtcac
     3001 gcggtacggc gcatgccgcg cataccgcgc cagaatggcg gcgatagcag caggccgagc
     3061 aacaggccaa acaattgcag cggtatcacg ctgacccggc cgccgctgtc gacctcgaat
     3121 acggcatcgg cgaattccgc ttgcagctcg atcgacgcgc cctcgacatt cgccagcaaa
     3181 ccggccaagg gaccgaataa tgcccagatt cttccggtat cggccgggtc accgaggccg
     3241 atacggattc gcaggtgcag atcccgtttg cgaaacgcgc accagaactg gcgtgcaaag
     3301 cgcagcatgc gccggcgaaa agctttttgc cgcagcgcgg ccagcggatc gagtttgcta
     3361 cgcgccggtt tcggtgtcgg cccgcccgcc ccgccggccc gcctgctgtt ggcggcaggc
     3421 ttgcgcgtcg gcgccaggcg cacgcgcacc aggccgaaca gccactgcag gctggcctcg
     3481 ccccgcaaac gctgccgcca gctgaactgg tatgtcaggc tgagcgggat cgacagcagg
     3541 gcgatcaaca ccagcacggt gaacacgatc gcagaccaca tcgcgacgcg cgccgatcaa
     3601 tcgctttgtt tgctgccctt ggcggcggcc accttgccga tcgactcggc gaccttctcc
     3661 agtaccgatg cggtgctgga cttgagctgt tcgacgcgca cgccatcggc ggtgatgatg
     3721 accaccgcta ccggcttgac accgccaccg ccaccggttc caccgccttc gccgctgccc
     3781 ttgttgggct ccgagccttt gccaccgccg acgccgaacc cgaagccgac gttgaccagc
     3841 gggatcagcg tggcgtcgcc ggcagtgatc ggttcgccaa ccacggtctt ggtgttgagc
     3901 atgcgctcga tctcggtaac cgccttatcg aatagttttt cgatgtcctg cattgcgacc
     3961 tccattttga tgagagattg cagttcagca ccgaaatcac cggtgggtat tgacgcaagt
     4021 cacgaccccg atctgcgagc agcggaattc gaattgacgg tgaccacggc aacccgtggc
     4081 cacgaaccgg gagcctgttt tcgcaacgag acgaggtcgt cccggcgatc gggtcacact
     4141 cgctgccgca gcctttcgcc ggaatgccga ggctgccgcc accgccgctg gccagctata
     4201 tcggaatctg ctggggtgcg ccattgccag atccccgctg gcgcagggtg accgcgggac
     4261 ttcggtatgc gggccttcgg caacggaggg attcggcatt gggcatgatt gaccgatggc
     4321 acgagtgcgg tcgggctccg cggatacggg tagcgaagac agattctggc gatagttctt
     4381 gaaatttata ccctaggggg gtatagtata cgccattcga tgcgcagcgg atggcgggcg
     4441 cgtcccgaac cgggaaaccg atatatgaac gatacactac gacagggcac gcagccggtg
     4501 agcgacggcg accaaggcat cgcaattcag tctttttatc ccgaccacaa gggccagttg
     4561 acccggctcg cccgtatcga gggccagatc aagggtatta cccgcatgat cgaggagcga
     4621 cgttactgtg tcgatatcgt gtcgcagatc aaggccgtgc aagcggcgct caagcaggtt
     4681 cagatgggcg tgctcgagaa gcacatacat cactgcgttg ccggcgcggc aaactcgggc
     4741 gatgcggcgc agctcaacga gaagatcgag gaaatcgtgc aggtcgtcgc caaactggat
     4801 tagtgaacgg gtgattcatg gttgcaagta aatctcgctt agcatccgaa atcggcgccg
     4861 aggcggcggc ttcgccgacg catggcgccc tgcagagaga gacgctgcgc gtcaccggca
     4921 tgacctgcgg cgcctgctcg gcgcgcgtcg agcgggtcgt cggcaagctc gacggagtca
     4981 ccgctgccag cgtcaacctg gccaccgaac gcatggacgt gcaattcgat acggcgcgca
     5041 tcgacctgcc gcagattcgc gccgccgtcg agaaggccgg ttacggaatc gaggaagccg
     5101 agcaggcgcg cgaaatcctg ttgccggtcg agggcatgac ctgtgccgcc tgcgcggcgc
     5161 gcatcgagaa agtcgtcggc agtctcgacg gcgtacgctc gatcgtggtc aacctgctcg
     5221 ccgataatgc caggattcgc tacctgcccg cggtgacctc actggcagcg attcgtgaag
     5281 ccatcgaagc tacgggctac cggacctatg aggtagtcga gagtagcggc agcgacgatc
     5341 gccagatgca gcacgacgat gcgctgcgcc gtcaacgcag agacctgctc gtggcactgg
     5401 cctttaccct gccgctgctg gcgatg
//
LOCUS       DATCZV010000392         2720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_667975, whole genome shotgun sequence.
ACCESSION   DATCZV010000392 DATCZV010000000
VERSION     DATCZV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667975"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VIW27_08845"
     CDS             <1..965
                     /locus_tag="VIW27_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5739819.1"
                     /translation="PTMGEEWRRGWHPERVPAAPQAASLLLVGGGPAGLEAALTLARR
                     GHRVTLAAAENQLGGRLLREASLPGLQNWIRVRDYRAGQLAQLENVDIYLESRLDTAE
                     IAEFDADHVVLATGSRWRRDGLGHNLRRPLANLDAANVYTPDDLFAGAVLSGEVLIYD
                     DDHYFMAGALAEQLLQSARRVRYVSPAASISSWTAMTNEQDFIQRRLLSMGIETAFGQ
                     LAAALDGQCFVTRCVYSGAITEHDFDQLLLVTSRVPEDRLFSSLEPSRTTRIGDCLVP
                     SSIADAVYAGHRFAREFGEQSSTLSPRRERSRLLPLASREEIPS"
     gene            962..2482
                     /locus_tag="VIW27_08850"
     CDS             962..2482
                     /locus_tag="VIW27_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5739820.1"
                     /translation="MKARTRRRAARKPRVSGSLSQPGWKLLRYRHPPFELLSAEAIED
                     IHQAGLTILRDTGMLVLSADARHRFAAAGCEVDTDSRLVRFDSAMLEETLASAPRSFR
                     LRARNPARDLVIGDGHGIFTSVGGPAYVMDLDRGRRNGSYDEMCDFIRLVQSLEILHQ
                     EGGGGFEAMDLPAASRHLDLFYAECTLLDKNWVPWTLGREQTLDAIEMVAISLGSSHQ
                     ELLAEPALCGIINTNTPLQLDVPMAEGLIAMAEHGQAVAVTPFTLSGAMAPVTLAGAL
                     ALQHAEAMAGIALTQIVRPGAPVLYGGFTSNVDMKTGSPAFGTPEYLQAAQASGQLAR
                     RLGVPLRSSNVNAANCPDAQAAYESCLSIWGAISGQASMIKHAAGWLHGGLTASLEKL
                     VIDAEVLQMVAASLEPFAVNPETLALEAIREVGPGGHFFGSAHTMERYQTAFYQPLLS
                     DWSNYESWLEAGAVSTEQRANRIWKQLLRDYQQPPIDASVDEALRDYVARRKREIG"
     gene            2485..>2720
                     /locus_tag="VIW27_08855"
     CDS             2485..>2720
                     /locus_tag="VIW27_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739821.1"
                     /translation="MNATQDNEDACDFRFRYLLDGPIVDVDAWPRLGGNANGPSLIRA
                     PEWLPNPPGRYLLYFAHHEGQSIRLAYSDDLSGP"
BASE COUNT          467 a          927 c          882 g          444 t
ORIGIN      
        1 acccgacgat gggcgaggaa tggcgtcgcg gctggcaccc ggaacgcgtg cccgcggcac
       61 cgcaggccgc gtcgctactg ctggtcggcg gcggcccggc cggtctcgag gcggcgctga
      121 cgctggcacg ccgcgggcac cgcgtgaccc tcgccgcggc cgagaaccaa ctcggcggcc
      181 gcttgttgcg cgaggccagt ctgccgggcc tgcaaaactg gattcgcgtg cgcgactacc
      241 gcgccggaca actcgcacag ctggaaaacg tcgacatcta cctcgaaagt cgcctcgaca
      301 ccgccgaaat cgccgaattc gatgccgatc acgtagtgct ggcgaccggc agccgctggc
      361 ggcgcgacgg actaggccac aacctgcgca ggccgctggc gaacctcgac gcggcgaacg
      421 tttacacgcc ggacgacctg ttcgccggcg ctgtcctgag cggcgaggtg ctgatttacg
      481 acgacgacca ttacttcatg gccggcgcgc tggccgagca gttactgcaa tccgcgaggc
      541 gtgtgcgtta cgtttcgccg gcggccagca tctccagctg gacggcgatg accaacgagc
      601 aggatttcat tcagcgccga ctgctgtcga tgggcatcga aaccgctttc ggccagctgg
      661 cggccgcgct cgacggtcag tgctttgtga cccgctgcgt ctacagcggc gcgatcaccg
      721 agcacgattt cgaccagttg ctgctggtca ccagccgcgt acccgaggac cggctgttca
      781 gttccctcga gcccagccgc acgacgcgta ttggcgattg cctggtaccg tcgagcattg
      841 ccgatgccgt ttacgccggc catcgtttcg cgcgcgagtt cggcgagcaa tcgtcgaccc
      901 tgtcgccacg ccgcgaacgc agccgcctgc tgccgcttgc aagccgcgag gaaataccgt
      961 catgaaagcc agaactcgcc gccgcgcggc gcgcaagccg cgcgtctccg gtagcctgtc
     1021 gcagcccggc tggaaactcc tgcgctaccg ccacccgccg ttcgaactgc tgagcgccga
     1081 ggccatcgag gatattcacc aggccgggct gacaatcctg cgcgataccg gcatgctggt
     1141 gctgtccgca gacgcgcgcc accgcttcgc cgcagccggc tgcgaggtcg atacggattc
     1201 gcgactggtg cgtttcgaca gcgcgatgct cgaggaaacc ctcgccagcg cgccacgcag
     1261 ttttcgactg cgcgcgcgca acccggcgcg cgacctcgtc atcggcgacg gtcacggtat
     1321 cttcacctcg gtgggcggtc ccgcctacgt catggacctc gaccggggcc gacgcaacgg
     1381 cagctatgac gaaatgtgcg attttatccg tctcgtacag agtctcgaaa tcctgcacca
     1441 ggaaggcggc ggcggcttcg aggccatgga cctgccggcg gcgagccgcc acctcgacct
     1501 gttttatgcc gagtgcacgc tgctcgacaa gaactgggtg ccgtggaccc tgggccgcga
     1561 gcagacgctc gatgcgatcg agatggtcgc gatcagtctc ggcagcagcc accaggaatt
     1621 actcgccgaa ccggcgttgt gcggcatcat caataccaac acaccgctgc agctcgatgt
     1681 accgatggcc gagggtctga tcgcgatggc cgagcacggc caggcggtcg cggtaacgcc
     1741 gttcaccttg tcgggcgcga tggcgccggt gacgctggcc ggcgccctcg cgctgcagca
     1801 cgccgaggcg atggccggca tcgcactgac gcaaatcgtg cgcccgggtg cgccggtact
     1861 ctatggcggc tttacctcca acgtcgacat gaagaccggc tcgccggcat tcggcacgcc
     1921 cgagtacctg caggccgcgc aggcctcggg gcagctcgcg cgccgcctcg gcgtgccgtt
     1981 gcgctcgagc aacgtcaacg ccgccaattg cccggatgca caggcggcct acgaaagctg
     2041 cctgtcgatc tggggcgcga tctccggcca ggcgagcatg atcaagcacg ccgccggctg
     2101 gctgcacggc gggctgaccg cgtcgctgga aaaactggtc atcgatgccg aggtgttgca
     2161 gatggtggcc gccagcctcg aaccgttcgc ggtcaatccc gagacactgg cgctggaagc
     2221 gattcgcgag gtcggaccgg gcggtcattt tttcggtagc gcgcatacca tggaacgcta
     2281 ccagaccgct ttctaccaac cgctgctgtc cgactggagc aattacgaga gctggctcga
     2341 agccggtgcc gtcagcaccg agcagcgcgc caatcgaatc tggaagcaat tgctgcgcga
     2401 ctatcaacag ccgccgatcg atgcgtcggt cgacgaggcg ctgcgggatt atgtcgcgcg
     2461 gcgcaaacgc gaaatcggtt gaatatgaac gctacgcaag acaacgaaga cgcttgcgat
     2521 ttccggtttc gttacctgct ggacgggccg atcgtcgacg tcgacgcctg gccgcgcctc
     2581 ggcggcaatg ccaacggccc gtcgctgatc cgcgccccgg agtggctgcc gaatccgccg
     2641 ggacgctacc tgctgtattt cgcccaccac gaggggcagt cgattcggct cgcttacagc
     2701 gacgatctca gcgggccgtg
//
LOCUS       DATCZV010000393         5793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_720899, whole genome shotgun sequence.
ACCESSION   DATCZV010000393 DATCZV010000000
VERSION     DATCZV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720899"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VIW27_08860"
     CDS             complement(<1..632)
                     /locus_tag="VIW27_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5739822.1"
                     /translation="MNPIKKTFQYGAHKVTLETGEIARQASGAVLINMADTVVMVTVV
                     GAKTADPGRDFFPLTVDYQEKTYAAGRIPGGFFKREGRPSEKETLTCRLIDRPIRPLF
                     PKGFMNEVQVVANVLSSNPEVDADVLAMLGTSAALAVSGIPFNGPIGACRVGYIDGDY
                     VLNPTASQLADSALNLVVAGTSNAVLMVESEANCLSEEVMLGSVVYGHEQ"
     gene            complement(647..916)
                     /gene="rpsO"
                     /locus_tag="VIW27_08865"
     CDS             complement(647..916)
                     /gene="rpsO"
                     /locus_tag="VIW27_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012068748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY5739823.1"
                     /translation="MAMSADQKAAIIGEFQQSKGDTGSPEVQVALLSHQISHLTEHFK
                     EHIHDHHSRRGLLKMVNQRRNLLDYLKSKNQQRYKDLIAKLGLRR"
     gene            complement(1030..1950)
                     /gene="truB"
                     /locus_tag="VIW27_08870"
     CDS             complement(1030..1950)
                     /gene="truB"
                     /locus_tag="VIW27_08870"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5739824.1"
                     /translation="MPRGKSGNYRDIDGFLLLDKPAGLSSNQALQRVRRIFRARKAGH
                     CGSLDPLATGLLPVCLGQATRFSGYLLGASKTYRARCRLGETTTTGDAEGEVVERRPP
                     TLASGQLQRVLSAFVGRIEQVPPMHSALKHQGRRLYQLAREGREVERAARTVEIQRLE
                     LLDCELPMIEIEVQCSKGTYIRSLAEDIGRELGCGAHLAALRRTEVDSMRVEDSLTLE
                     DLQALAADDPQALEATLMPPDAALEGYPCIELQAAAARDICHGKRVDLACEILPGLYR
                     LMAADGDFIGLGEVRDGGELAAKRLMNTAG"
     gene            complement(1952..2320)
                     /gene="rbfA"
                     /locus_tag="VIW27_08875"
     CDS             complement(1952..2320)
                     /gene="rbfA"
                     /locus_tag="VIW27_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY5739825.1"
                     /translation="MPRDYPRSERLASQIQRELALLLHSGIKDPGLGMPSILGVEVSK
                     DLAHARVFFSVLDPQNAVACAEALERARGFLQREIGKQLKTRVTPKLSFVYDDTDIRG
                     RQLSDLIDSAIASDKHKSGQ"
     gene            complement(2333..4903)
                     /gene="infB"
                     /locus_tag="VIW27_08880"
     CDS             complement(2333..4903)
                     /gene="infB"
                     /locus_tag="VIW27_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5739826.1"
                     /translation="MANVTAKQLSEVIGVSIEKLLDQLNTAGVQVSSADDPISDEDKL
                     KLLESLRSSHGRDAGEGPKKITLRRKSKSELRVSGTSGRNTTTKTINVEVRKKRTYVR
                     RADVEAQEVHQQQELEAEAEAERQAKLEQERAEAEAKEIADRAAAEEAARAEAEKKAY
                     EKSLQEVEAAQKAIEKERAEAEAAQAAEAAARAALEQAATASAAATTAEKTDKPGKHG
                     KHEDRGTRYGRKELHVKDSGHHASRKQKFKKGQRQVAEIAKTHQFEMPTAPVVRNVEI
                     PDAISVADLAQRMAVKAGDVIKVLMQMGTMATINEILDRDTASLVVEEMGHNVVTAAS
                     DDDFEKELQVSAEQQGVAKVSRPPVVTIMGHVDHGKTSLLDYIRESRVTSGEAGGITQ
                     HIGAYHVETDKGIVTFLDTPGHAAFSAMRARGAQATDIVVLVVAADDGVMPQTKEAIQ
                     HSRAAGVPIVVAVNKIDKEDADPDRVKNELAAMDVIPEDWGGDTIFVNVSAITGEGVD
                     KLLDSILLQAEILELEAVDKGNAKGIVVEASLDKGRGPVATVLVQSGLLQQGQMLLAG
                     TQYGRVRAMYDENARSIKSAGPSMPAEVLGLSGVPNAGDEVFVVENERKAREFAQIRE
                     AKIRESKLADQQKAKLQGMFDAMAEGEVAEINILVKADVQGSAEAIRESLEKLSTDEV
                     RTKLVATGVGGINESDINLAAASNAVVIGFNVRADSAAKRIAGDHGIDIRYYSVIYDI
                     IDDIKVIMSGMLAPESQENIIGLAEVKEVFRSPKFGDVAGSIVIEGVIRKGRPIRVLR
                     DNVVIYEGELESLRRFKDPVEEVRMGTECGIAVKNYNDVKPGDQIEVFERVEVARTIE
                     "
     gene            complement(4945..>5793)
                     /locus_tag="VIW27_08885"
     CDS             complement(4945..>5793)
                     /locus_tag="VIW27_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEY5739827.1"
                     /translation="EVGQELIEIISAARDPGMRAKIAVKSNDPRMDPVGACVGMRGSR
                     VQSVSNELAGERVDIILWDENPAQFAVNAMSPAEVTSIVIDEETHSMDIAVPEEKLSQ
                     AIGRGGQNIKLASQLTGWTLNVMDEAAAEEKSEGESRQLVENFMTQLDVDEELAIILV
                     QEGLTTIDEVAYIPESELLEIEEFDDEIVQELRGRARDALLTMAIAREETAEAGEPQD
                     DLLNMEYMTSELAHKLSRRGICTMEDLAEQSTDELEEIEGIDAEFAGKLIMKAREPWF
                     AEAEAE"
BASE COUNT          945 a         1886 c         1719 g         1243 t
ORIGIN      
        1 atctgctcgt ggccatagac caccgaaccc agcatcacct cttcggacag gcaattggct
       61 tccgattcga ccatcagcac ggcattgctg gtacccgcga cgaccaggtt gagcgcggaa
      121 tccgccagtt ggctggcggt cgggttgagc acgtaatcac cgtcgatata gccgacccgg
      181 caagcgccga tcggaccgtt aaacggaatg ccggaaaccg ccagagcggc cgaggtgccg
      241 agcatcgcca gtacgtcggc gtcgacttcc ggattgctgg aaagcacatt ggcgaccacc
      301 tggacttcgt tcatgaagcc tttcgggaac aacgggcgaa tcggccggtc gatcaggcgg
      361 caggtcaggg tttctttttc gctgggacgg ccctcgcgct tgaagaaacc gccgggaatg
      421 cggcctgcgg cgtaagtctt ttcctggtaa tcgacggtca gcggaaagaa gtcgcgtccc
      481 gggtcggcgg tcttggcgcc gaccacggtc accatcacta ccgtgtccgc catattgatg
      541 agtactgcgc cgctggcctg gcgcgcgatt tctccggtct ccagcgtgac tttgtgggcc
      601 ccgtactgga aggtcttttt aattggattc actattttct cactgtttaa cgacgcaatc
      661 cgagcttggc gatcaggtcc ttgtaacgct gctgattctt ggatttcaga taatccagca
      721 aattgcgacg ctggttgacc attttcagca gtccgcgacg cgaatgatgg tcatgaatgt
      781 gttccttgaa atgttcggtc aggtggctga tctggtggga cagcagcgca acctgtacct
      841 cgggtgaacc ggtatcaccc ttgctttgct gaaattcacc aataatcgca gccttttggt
      901 cagctgacat tgccatgaaa attcctcgtg gacccgaaaa aggcaggtat tctacctatc
      961 tgcgcaagtg gtaacaagga ctttcaccgg aattctccgc cgaattggcc cgggccaccg
     1021 caggtagagc tatcctgctg tattcattaa tctttttgct gccaattcgc cgccgtcgcg
     1081 gacttccccg agaccgatga agtcaccgtc ggcggccatc agtcgataca gccccgggag
     1141 gatctcgcag gccagatcga cgcgcttgcc gtggcagata tcacgcgccg cggcggcttg
     1201 caactcgatg cagggatagc cctcgagggc cgcgtccggc ggcatcagcg tggcctccag
     1261 cgcctgcgga tcgtcggccg ccagcgcctg taaatcctcc agggtcaggc tgtcctcgac
     1321 tcgcatgctg tcgacctcgg tacggcgcaa cgcggcgagg tgagcgccgc agcccagctc
     1381 gcggccgata tcctcggcaa ggctgcgtat ataggttccc ttggagcact gcacctcgat
     1441 ttcgatcatc ggcaattcgc aatccagcaa ctccagccgc tgaatctcga ccgtgcgcgc
     1501 ggcacgttcg acctccctgc cctcgcgggc cagctggtaa agccgccggc cttggtgctt
     1561 gagcgccgag tgcatcggcg gaacctgttc gatacggcca acgaaagccg acaaaacccg
     1621 ctgcagctgc ccgctggcga gcgtcggcgg acgccgctcg acgacctcgc cctcggcgtc
     1681 gccggtggtg gtggtctcgc cgagccggca acgcgcgcgg taagtcttgc tggcgcccag
     1741 caggtaaccg gaaaagcggg tcgcctggcc caggcagacc ggcaacaggc cggtggccag
     1801 cggatcgagg ctgccgcagt gaccggcctt gcgagcgcgg aaaattcggc gcactcgctg
     1861 cagcgcctgg ttggaactca gcccggccgg cttgtcgagc agcaggaacc catcgatatc
     1921 acggtaattc ccggacttgc cgcgcggcac cctactgccc cgatttgtgc ttgtcgctgg
     1981 cgatcgcgga atcgatcagg tcggataact ggcggccacg aatgtcggta tcgtcgtaaa
     2041 cgaaactgag cttcggcgtt acccgggtct tcaactgctt gccgatctcg cgctgcagga
     2101 aaccgcgcgc acgctccagc gcctcggcac aggcaacggc attttgcggg tcgagcacgc
     2161 tgaagaacac ccgcgcatgg gcgagatcct tgctgacctc gacgccgagg atgctcggca
     2221 tgccgagccc ggggtccttg atgccgctgt gcagcaacaa cgcgagttcg cgctgtatct
     2281 gcgatgcaag ccgttcgcta cgcgggtaat ccctgggcat cgccagcgtc gcttattcga
     2341 tggtgcgcgc gacttcgacg cgctcgaaga cctctatctg gtcgcccggc ttgacatcgt
     2401 tgtagttctt taccgcgatg ccgcactcgg tgcccatacg aacttcctcg accgggtcct
     2461 tgaagcggcg cagcgattcg agttcgccct cgtagatgac gacattgtcg cgcaacacgc
     2521 gaatcggccg gcccttgcgt attacgccct cgatcacgat cgatcccgcg acatcgccga
     2581 acttcggcga acggaacact tccttgacct cggccaggcc gatgatgttt tcctgcgact
     2641 ccggcgccag catgccggac atgatcacct tgatatcgtc gatgatgtcg tagatgacac
     2701 tgtagtaacg aatatcgata ccatgatcgc cggcaatgcg cttggcggcc gagtcggcac
     2761 gcacgttgaa gccgatgacc accgcgttgg acgccgccgc caggttgata tccgattcgt
     2821 tgataccgcc gaccccggtg gccaccagct tggtgcgcac ctcgtcggtg gacagctttt
     2881 ccagcgattc gcgaatcgcc tcggcgctgc cctgtacgtc ggccttgacc aggatattga
     2941 tctcggcgac ctcgccctcg gccatcgcgt cgaacatgcc ctgtagcttg gccttttgct
     3001 ggtcggcgag cttggactcg cggatcttgg cttcacgaat ctgcgcgaat tcgcgcgcct
     3061 tgcgctcgtt ctcgaccacg aagacctcgt cgccggcatt cggcacgccg gaaagcccga
     3121 gcacctcggc cggcatcgac ggccccgccg acttgatgct gcgggcgttt tcatcgtaca
     3181 tcgctcgcac gcggccgtac tgggtgccgg cgagcagcat ctggccctgc tgcagcagcc
     3241 ctgactgtac cagcaccgtg gccaccggac cgcggccctt gtcgagcgaa gcttcgacaa
     3301 cgataccctt ggcattgcct ttgtcgacag cctcgagctc gagaatctcg gcctgcaaca
     3361 ggatcgagtc cagcagcttg tcgacgccct ccccggtgat tgccgagacg ttgacgaaaa
     3421 tggtatcacc gccccaatcc tcgggaatca cgtccattgc cgccagctcg ttcttgacgc
     3481 ggtcgggatc ggcatcttcc ttgtcgatct tgttgaccgc gaccacgatc ggcacgccgg
     3541 cagccctgga gtgctggatc gcctccttgg tctgcggcat gacgccatcg tcggcggcaa
     3601 ccaccagcac cacgatatcg gtggcctggg cgccgcgcgc gcgcatcgcg gaaaacgcgg
     3661 catggcccgg ggtatcgaga aaggtcacga tgcccttgtc ggtttccacg tgataggcac
     3721 cgatgtgctg ggtgatgcca ccggcctcgc cggaggtgac gcgcgattcg cggatgtagt
     3781 ccagcagcga ggtcttgccg tgatcgacgt ggcccatgat ggtcaccacc ggcggccggc
     3841 tgaccttggc gacgccctgc tgctcggcgc ttacctgcag ttccttctcg aaatcatcgt
     3901 cgctggcggc ggtgaccacg ttatggccca tctcctcgac gaccagcgag gcggtgtcgc
     3961 ggtcgagaat ctcattgatg gtggccatgg tgcccatctg catcagcacc ttgatgacat
     4021 cgccggcctt gacggccatg cgctgcgcga gatcggcgac cgagatggcg tcgggaatct
     4081 cgacgttgcg caccaccggc gcggtcggca tttcgaattg gtgggtcttg gcgatttccg
     4141 ccacctgccg ctggcccttc ttgaacttct gcttgcggct ggcgtggtgc ccggaatcct
     4201 tgacgtgcaa ttccttgcgc ccgtagcgcg tgccacggtc ttcgtgtttg ccgtgcttgc
     4261 cgggcttgtc ggttttctct gcggtagtcg cggccgcgct ggccgtggcg gcctgttcga
     4321 gcgcggcgcg cgccgcggct tccgcggcct gcgcagcctc ggcctcggcc cgttcttttt
     4381 cgatcgcctt ttgcgcggcc tcgacttcct gcaggctttt ctcgtaggcc ttcttctcgg
     4441 cttccgcgcg cgcggcttcc tcggccgccg cacgatccgc gatctctttt gcctcggcct
     4501 cggcccgctc ctgctcgagc ttcgcctgcc gttccgcttc ggcttcagcc tccagttcct
     4561 gctgctggtg gacttcctgg gcctcgacat cggcacgacg cacgtaggta cgcttcttgc
     4621 gcacctcgac attgatcgtc ttggtagtgg tattgcggcc ggaggttccc gacaccctga
     4681 gctcgctctt gctcttgcgc cgcagggtga tctttttcgg cccctcgccg gcatccctgc
     4741 catggctact gcgcagcgac tccagcaact tcagtttgtc ctcgtcggag atcgggtcat
     4801 ccgcgctgct gacctggaca cccgcggtgt tcagctgatc gagcagcttt tcgatactga
     4861 cgccgatgac ttccgaaagt tgtttcgctg ttacgtttgc catgtttatc cttccacttg
     4921 cctgttctgc cgcggaccgc gtccctattc ggcctcggct tccgcgaacc agggttcgcg
     4981 ggctttcatg atgagcttgc cggcaaactc ggcatcgatg ccctcgattt cctcgagttc
     5041 atcggtcgac tgttcggcga gatcttccat cgtgcagatc ccacggcgcg acagtttgtg
     5101 cgcgagttcg ctggtcatgt attccatgtt gagcagatca tcctgcggtt cgccggcctc
     5161 ggcggtttcc tcgcgtgcga tcgccatcgt cagcagggca tcgcgcgcac gaccgcgcaa
     5221 ctcctgtacg atttcatcgt cgaactcctc gatttcgagc aattcggact cggggatgta
     5281 agccacttcg tcgatggtgg tcaagccttc ctgcaccagg atgatcgcca gttcctcgtc
     5341 gacatcgagc tgggtcatga aattctccac cagctggcgg gactcaccct cggatttttc
     5401 ctcggccgcc gcctcgtcca tgacgttcag cgtccagccg gtcaactggc tggcgagttt
     5461 gatattctgc ccgccgcggc cgatggcctg cgacagtttt tcctcgggca ccgcgatatc
     5521 catcgaatgg gtttcctcgt cgatgacgat cgaggtgacc tcggccggcg acatcgcgtt
     5581 gaccgcaaac tgcgccgggt tttcgtccca cagaatgata tcaacgcgct cgccggcgag
     5641 ctcgttggat accgactgca cgcgcgaacc acgcatgccg acgcaggcgc cgaccgggtc
     5701 catgcgcggg tcgttactct ttaccgcgat cttggcacgc atgccggggt cgcgggccgc
     5761 ggaaatgatc tcgatcagct cctggccgac ctc
//
LOCUS       DATCZV010000394         3969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_615041, whole genome shotgun sequence.
ACCESSION   DATCZV010000394 DATCZV010000000
VERSION     DATCZV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615041"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..595
                     /locus_tag="VIW27_08890"
     CDS             158..595
                     /locus_tag="VIW27_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY5739828.1"
                     /translation="MAWWGWMIVGALLLGSELLAVDAGFYLVFIGIAAAITGLIELAG
                     ASLEPWVQWILFSAIALVLMVLFRKKLYARLRGSGEGYVTGPAGETITLPQALQPGES
                     SRLAYRGTDWTIVNDSTGPFAQGQRVRIRQVDGLTLKLDKIED"
     gene            599..1546
                     /locus_tag="VIW27_08895"
     CDS             599..1546
                     /locus_tag="VIW27_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEY5739829.1"
                     /translation="MFDFYGNVSFWVAIAVSIVVIIVIFKTAVVVPQQTAVVIESLGK
                     YSRTLRAGFHILIPFIESAAYRHSLKELAIDIPEQVCITRDNVQVGVDGVLYLQVMDP
                     ERASYGITDFVFAISQLAQTTLRSEIGKIDLDRTFEERGAINANVVAELDKASDPWGV
                     KVLRYEIKNINPPHDVLTAMEKQMRAEREKRAVILTSEGVRDAKINEAEGEKQRVIKE
                     SEAAKQQQINEAEGEAEAILAVAGATAEGLRRVAESLITQGGDQAMQLRVAEAYIEQF
                     GKLAKEGNTLVVPSNLSDLASMIALATTVVRKEPPATAT"
     gene            1543..2196
                     /locus_tag="VIW27_08900"
     CDS             1543..2196
                     /locus_tag="VIW27_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011377433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY5739830.1"
                     /translation="MSSSLFDLVIFDCDGVLVDSERLANEVFAGILREVCGLDFSLDE
                     MFDTFVGHSKAQCLAKIEAMIGEPPPDELERRYREDINQALASSVQAIDGIEAVLAQL
                     ALPCCVASSGSHDKMRLTLGKTGLIGYFDGNIFSTSEVERGKPHPDIYLHAAARMGVS
                     DPGRCVVIEDSPLGVTGAVSAGMRVYGFAELMSARKLHDAGAHKVFDRMSDLPSLIA"
     gene            2370..>3969
                     /gene="mnmG"
                     /locus_tag="VIW27_08905"
     CDS             2370..>3969
                     /gene="mnmG"
                     /locus_tag="VIW27_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY5739831.1"
                     /translation="MSFNTRFDVIVVGGGHAGTEAALAAARMGAATLLLTHSIETLGQ
                     MSCNPAIGGIGKGHLVREIDALGGEMALAADRAGIQFRILNRRKGPAVRATRAQADRQ
                     LYRMAIREKLENQPGLAIFQQPVVDLIENGASIDGVITQMGLRFYAPAVVLTVGTFLD
                     GKIHIGDTNYAGGRAGDPPAKALAERLRARPFRVDRLKTGTPPRIDRKTIDFSRLEEQ
                     PGDDPLPVFSTMGSVDMHPEQVSCHITHTNSRTHDIIRDSLHRSPLYSGEIEGVGPRY
                     CPSIEDKVVRFADKASHQVFVEPEGLKLGEVYPNGISTSLPFDVQQRVVNSMAGFERA
                     QITRPGYAIEYDFFDPRDLQPTLETRFIGGLYFAGQINGTTGYEEAAAQGLLAGANAA
                     ARVLGRPALTLQRSEAYLGVLVDDLITRGTGEPYRMFTSRAEYRLMLREDNADLRLTE
                     RGRRLGLVEDARWRAFEDYRERLERLEQALNDQWLRPQTPAAQALQEKLSAPLAREFR
                     AAEVLKRPEISIDDVMPLLPAGEAY"
BASE COUNT          749 a         1228 c         1260 g          732 t
ORIGIN      
        1 gatccggagt cggtaagggt gcgttacctg tatactctga cttgctgacg ggctgaatcc
       61 gcatcgggca agcaggtatc gcgtccccgg aattctttcc gcttgcgtgc tccagatagc
      121 ataactaata tgaatcgcca cgcaacggga gaaccctatg gcctggtggg gatggatgat
      181 tgttggcgcc ttactgctcg gttcggaact gctggcggtg gatgcgggat tttatctggt
      241 cttcatcggc atcgccgccg cgataaccgg cctgatcgag ttggccgggg ccagcctcga
      301 accctgggtg cagtggattc ttttttcggc gatcgcgctg gtactgatgg tgctgttccg
      361 caaaaaactc tacgccagac tgcgcggttc gggcgagggc tacgtcaccg gccccgccgg
      421 tgaaacgatc accctgcctc aggctttgca gccgggcgaa agcagccggc ttgcctaccg
      481 cggcaccgac tggacgatcg tcaacgacag caccgggccc tttgcgcagg gtcagcgcgt
      541 gcgcatccgg caagtcgacg gcctgacatt gaaactggat aaaatagagg attaacaagt
      601 gttcgacttt tatggaaatg tttcgttctg ggttgcgatc gcggtttcga tcgtcgtcat
      661 catcgtcatt ttcaaaaccg cggtggtggt gccgcagcaa accgccgtgg tgatcgaaag
      721 cctcggcaag tacagccgca cgctgcgcgc cggttttcat atactgattc cgttcatcga
      781 atcagccgcc taccggcaca gcctcaagga gctggccatc gatatcccgg aacaggtctg
      841 tattacccgg gacaacgtgc aagtgggcgt cgatggcgtg ctctatctgc aggtcatgga
      901 tcccgagcgc gcctcctacg ggattaccga tttcgtgttc gcgatatctc agctggcgca
      961 gacgacattg cgcagcgaga tcggcaagat cgacctggac cgtaccttcg aagagcgcgg
     1021 tgcgatcaac gccaatgtcg tcgccgaact cgacaaggcc tcggatccct ggggagtcaa
     1081 ggtgctacgc tacgagatca agaatatcaa cccgccccac gacgtgctga cggcgatgga
     1141 aaagcagatg cgggctgaac gcgaaaagcg cgcggtgatc ctgacctcgg aaggcgtgcg
     1201 ggatgccaag atcaacgagg ccgagggtga aaagcagcgt gtcatcaagg agtccgaagc
     1261 cgccaagcaa caacagatca acgaggccga aggcgaggcc gaggcgatcc tcgcggtggc
     1321 cggcgcgacc gccgagggtt tgcggcgagt agccgaatcg ctgatcacgc agggtggcga
     1381 ccaggcgatg cagctacgcg tcgccgaggc ttatatagag cagttcggca agctggccaa
     1441 ggaaggcaac acgctggtcg tcccgtccaa cctgtccgac ctggcgtcga tgatcgcgct
     1501 cgccaccacc gtggtcagaa aagaaccccc cgctaccgcc acttgagcag cagcctgttc
     1561 gatctcgtta tcttcgattg cgacggggtg ctggtcgaca gcgagcgcct cgccaacgaa
     1621 gtctttgccg gtatcctgcg cgaagtctgc ggtctcgatt tcagcctcga cgagatgttc
     1681 gacacctttg tcggccactc gaaggcccag tgtctggcga agatcgaagc gatgatcggt
     1741 gagccgccgc cggacgaact cgaacgacgc tatcgcgaag acatcaatca ggctctggcc
     1801 agttcggtac aggcaatcga cggtattgag gcggtgctcg cgcaactcgc gctgccgtgt
     1861 tgtgtcgctt ccagcggttc ccatgacaag atgcgcctga cgctcggcaa gaccgggctg
     1921 atcggctatt tcgacggcaa tattttcagt accagcgagg tcgagcgggg caaaccgcat
     1981 ccggatatct acctgcacgc ggccgcgcgc atgggcgtat ctgatccggg ccgctgcgtg
     2041 gtgatcgagg acagcccgct cggggtgacg ggcgcggtgt cggccggcat gcgcgtgtac
     2101 ggtttcgccg agctgatgtc ggcccgcaag ctgcatgatg ccggcgccca taaagtcttc
     2161 gatcgcatgt ccgatcttcc gtcgctgatc gcctagcgtt tttgagctgg tcccagacct
     2221 ggccgattaa tttggatctg acccaaatta aaggatgcga tttttcggct ggcgaaaaag
     2281 tgactctggc gctgttttcg gggtggctgg gttttggaat cgccggtaat tgagctagaa
     2341 tgcgcgccct tttcggttaa tatggtgcga tgagcttcaa cacgcgtttc gatgttatcg
     2401 tggtcggcgg cggtcatgcg ggtaccgagg ccgcgctcgc ggccgcgcgc atgggagccg
     2461 cgacgctgtt gctgacccac agtatcgaaa ccctcggcca gatgtcctgc aatcccgcca
     2521 tcggcggcat cggcaagggc cacctggtgc gcgaaatcga cgcgctcggc ggcgagatgg
     2581 cgctggccgc cgatcgagcc ggtatccagt ttcgcattct caatcgccgc aaaggcccgg
     2641 cggtacgcgc gacgcgggcg caggcggacc gccagctgta tcgcatggcg attcgcgaaa
     2701 agctcgaaaa ccagcccggg ctggcgatct tccagcagcc ggtcgtcgac ctgatcgaga
     2761 acggcgcctc gatcgacggc gtgattacgc agatgggcct caggttttac gcgccggcgg
     2821 tggtgctgac cgtcggcacc tttctcgacg gcaagatcca tatcggcgat accaactatg
     2881 ccggcggccg tgccggcgat ccgcccgcca aagcgctcgc cgagcgcctg cgcgcgcggc
     2941 cgtttcgcgt cgaccggctg aaaaccggca cgccgccgcg tatcgaccgc aagaccatcg
     3001 atttctcgcg gctcgaggaa cagcccggcg acgacccgtt gccggtgttc tccaccatgg
     3061 gctcggtcga catgcacccc gagcaggtca gctgccacat cacgcacacc aacagcagaa
     3121 cgcatgacat cattcgcgat agcctgcacc gctcgccgct ctacagcggc gaaatcgagg
     3181 gcgtcggacc gcgctactgc ccgtcgatcg aggacaaggt ggtgcgtttc gcggacaagg
     3241 cctcgcacca ggtattcgtc gagcccgagg gcctgaaact cggcgaggta tatcccaacg
     3301 gaatatcgac cagcctgccg ttcgacgtgc agcagcgcgt cgtcaacagc atggcgggtt
     3361 tcgagcgcgc gcagatcacc cgcccgggct acgccatcga gtacgacttc ttcgatccgc
     3421 gcgacctgca gccgacgctg gaaacgcgtt ttatcggcgg gctgtacttc gccgggcaga
     3481 tcaatggcac caccggctac gaggaagctg ccgcgcaggg cctgctggcc ggtgccaacg
     3541 ccgccgcgcg cgtgcttggc aggccggcgc tgaccctgca gcgctccgag gcttatctcg
     3601 gcgtgctggt cgacgacctg atcacccgcg gcaccggcga gccctaccgc atgttcacct
     3661 cgcgcgccga gtaccggttg atgctgcgcg aggacaatgc cgacctgcgc ctgaccgagc
     3721 gcggccgtcg actcggcctg gtcgaagatg cccgctggcg cgctttcgag gattaccgcg
     3781 agcgactcga gcgactcgag caggcgctca acgatcaatg gctgcggccg cagaccccgg
     3841 cggcgcaggc gctgcaggaa aagctgtcgg cgccgctggc gcgcgaattc cgcgcggcgg
     3901 aagtcctcaa gcggccggag atcagcatcg acgatgtcat gccgttgctg cccgccggcg
     3961 aggcttacc
//
LOCUS       DATCZV010000395         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_350508, whole genome shotgun sequence.
ACCESSION   DATCZV010000395 DATCZV010000000
VERSION     DATCZV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350508"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1484
                     /locus_tag="VIW27_08910"
     CDS             255..1484
                     /locus_tag="VIW27_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEY5739832.1"
                     /translation="MKIKNPTRISIRQLLSAAGLALLLLLACRSAIASADNAFTLAVI
                     PDTQNYIDFRHQKAKGFEFDAADLFIQQMRYVAGRSVNNGGDIAFLAAVGDVWQHQTR
                     TIDKDHLTRGIGIEPNPILGRRSKRADEVLNIELPRATEGYRIISEAGLPFGVAPGNH
                     DYDATWSVIGYPPNRQKKWSELSRTVEDLGIMHIGGLDNFRSVFGADSDFFRDKPWYI
                     GSYNGGSSSAQRFDAGGYEFLHITLEMQPGDAVLQWAESIIAANPGLPTIVTTHDYPS
                     TSGKRLPNPLIDLARIEPENHNSAEQLWRKLIRRHDQIFLVLCGHQHGQSFRVDDNLD
                     GNPVYQILADYQVRGASRGAGWAQAAGRHRRWLAAPDDFRLCHQPGDDYRQDLLASTH
                     RTWIAMLTGTGLRSSRG"
     gene            1574..>2636
                     /locus_tag="VIW27_08915"
     CDS             1574..>2636
                     /locus_tag="VIW27_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5739833.1"
                     /translation="MKRLEAQLRLATGLILAVYVLQHLVNHAFGIASIEAAEAYRKTV
                     GALFQNLPGLVLLYGSILFHALIALRSIYRRSSLRMSLWQWLQLLLGLSILPLLVGHT
                     VGNRGFDLLGDIDPNYYYVVTSFLLSPAFLVKLVILIPVIWAHMAIGLHFWLRIKDGY
                     DRFVPYIYALAILVPALAYVGLFRMLLQASGWLDDQARLDQIYAANIAMSRDDVEFLR
                     GLEAQAWTVMAALLILTLAARQFRIWIQARQGSYSITQADGNKIRAMQGVSLLEALRN
                     ARIPHASVCGGRARCTTCRVRVGSGLDQLDPPNELEARALARIRAGKEIRLACQLHPK
                     SDLAITPLVMANQPLASALH"
BASE COUNT          522 a          799 c          782 g          533 t
ORIGIN      
        1 accagggcga tagcaaaata gagaaagtac tgtaacccga tttgctcacc taccgtgtcg
       61 cgcagcgcgc gctaccggaa tcgtgctaaa gacgattacg acgcccagcg cgacataaca
      121 ccaggacagt ttttcggatt ctctgggtgg cgagtcgagc aagcgaccta acctcctgcc
      181 agggttaaat tactcccgtc caacctgtac cggcagcggg tttcggttta tatttggcgg
      241 cattctagca agccatgaaa ataaagaatc ctacacgtat ctcaattcgc cagctattgt
      301 cggccgcggg cctggcattg ctgttgttac tggcgtgccg gtcggcgatt gcatccgcgg
      361 acaatgcctt cacgctggcg gtaattccgg atacccagaa ttatatcgat ttcaggcatc
      421 agaaagccaa aggcttcgag ttcgacgcgg ccgacctgtt catccagcaa atgcgttacg
      481 tcgccgggcg cagcgttaac aacggtggcg atatcgcctt cctcgccgcg gtcggcgacg
      541 tctggcagca ccagaccagg accatcgaca aggaccattt aacccgcggc atcggtatcg
      601 agccaaaccc catactggga cggcgctcaa agcgcgccga tgaagtgttg aacatcgaac
      661 tgccgcgggc aaccgagggt taccgcatta tcagcgaggc cgggctaccg ttcggcgtgg
      721 cccccgggaa tcatgactac gacgccacct ggagcgtcat tgggtatccg cccaaccgcc
      781 aaaagaagtg gtcagaatta tcgcgcacgg tcgaagacct cggcatcatg cacatcggcg
      841 ggctcgacaa ttttcgcagt gtattcggcg ccgacagcga cttcttccgc gacaaaccct
      901 ggtatatcgg gagttacaac ggcggcagta gcagcgcgca gcgcttcgac gccggcggct
      961 acgaattcct gcacattacg ctcgagatgc aacccggcga cgcggtgctg caatgggcgg
     1021 agtcgattat tgccgcaaat ccgggcttgc cgacgatcgt aacgacccac gactacccga
     1081 gcacatcagg caaacgtcta cccaatccat tgatcgatct ggcacgtatc gagcctgaaa
     1141 atcacaacag cgccgagcag ctgtggcgca aactgatccg ccggcatgac cagatcttcc
     1201 tggtgctttg cgggcaccag cacggccaga gttttcgcgt cgacgacaat ctcgacggta
     1261 acccggtcta ccagatcctg gccgactacc aggtgcgggg ggcaagtcgc ggtgcaggct
     1321 gggcgcaagc ggcgggtcgg catcggcgat ggctggctgc gcctgatgac tttcgacttt
     1381 gccaccagcc cggcgacgat taccgtcagg acctattggc cagtactcat cggacatgga
     1441 tcgctatgct gactggtacc gggctaagga gcagccgcgg atgagcgacg ccgagtttat
     1501 ggccgcggac gatttcttga tccggctcga gggtttcaag caccgattcg cgcgcgcgcg
     1561 tcaggccgag taagtgaaac gattagaagc ccagctgcgg ctcgccaccg gcctgatcct
     1621 ggcggtctac gtgctacagc acctggtcaa ccatgctttt ggcattgcct cgatcgaagc
     1681 cgccgaagcc taccgcaaga cggtcggcgc cctgtttcag aatttgccgg gtctggttct
     1741 gctgtatgga tcgatcctgt ttcacgcgct gatcgcgctg cgttcgattt atcggcgctc
     1801 gagcctgcgc atgtctttgt ggcaatggct gcaactgctg ctcggacttt cgatcctgcc
     1861 gctattggtc gggcatacgg tcggcaaccg cggcttcgat ctgctcggtg acatcgatcc
     1921 taattactat tacgtcgtga cctcgttctt actgagtccc gcgttcctgg tgaagctggt
     1981 tatattgatc ccggtaatat gggcgcacat ggccatcggc ctgcatttct ggctcaggat
     2041 caaggacggc tatgatcgat tcgtgcctta catctacgcg ctggcgatac tggttcccgc
     2101 gctcgcttac gtcggcctgt tccgcatgct gctgcaggcc agcggctggc ttgacgatca
     2161 ggcacggttg gaccagattt atgccgccaa catcgcgatg agccgtgacg acgtcgagtt
     2221 tttgcgcgga ctggaagcgc aagcctggac ggtgatggcg gcgctgttga tcctgaccct
     2281 ggctgcgcgc cagttccgaa tctggataca ggcgcgccag ggcagctaca gcatcaccca
     2341 ggccgatggc aataagatca gggccatgca gggagtgtcc ctgctcgagg cgttgcgcaa
     2401 cgcgcgcatt ccgcacgcct cggtttgcgg cgggcgcgct cgctgcacca cctgccgggt
     2461 gcgggtcggt agcggcctgg accagctcga ccctccgaat gagctggaag cgcgcgcgct
     2521 ggcgcgtatc agggccggca aggagattcg cctggcctgc cagctacatc cgaaatccga
     2581 tctcgcgatc acgccgctgg tgatggcgaa ccagccgctc gccagcgcgc tgcatg
//
LOCUS       DATCZV010000396         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_456539, whole genome shotgun sequence.
ACCESSION   DATCZV010000396 DATCZV010000000
VERSION     DATCZV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456539"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..2431
                     /locus_tag="VIW27_08920"
     CDS             269..2431
                     /locus_tag="VIW27_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007801748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5739834.1"
                     /translation="MVAGFVVGQRVPFNALEIVWDRKQLLNLALMYLVFFVPFFFAAC
                     CVGLALTCRSEAISRLYFCDLAGAGTGAVLIIGVLLVAFPQSALIVLVAFVLIASVLV
                     GNSTAARVPLSVGQFTWLVALAIIVFNGGLTLRPSEFKGLSQALQVVDARALTQVSSP
                     LGLLTVVDSPTVPIRLAPGLSFNTRHVPPEQLAVFTDADGMSAITQYDGDRESVAFLA
                     DVTAALPYALLEQPDVLILGAGGGSDVLLALYHGANHVDAVELNPRMTELVADTYAEF
                     AGHLYDDPRVDLHTAEARGYVAQNDRRYDLIQIGLLDSFAAAGAGVQALNESYLYTVE
                     AVGEYLADLNPGGVVAITRWVKMPPRDSLKLAAIAIEALEQAGEVDPGRRIAVIRSWN
                     TVTLLIRRGAFVPSEIDAMREFASSRSFDTAYFPGITSADANRFNLLDRPWLYDGIQA
                     MLGDSADRFFADYKYFVEPATDDRPYFSHFFKWRSLPEVMAMRASGGAGLIEWAYLIV
                     VTTLVQAAVIGGLLILLPLSLMKRTWVGGTRFGAYFLALGLAFLFVEIAFIQRFTLFL
                     SHPLYAVAVVLASFLVFAGAGSAYSLRLADRAIARNVRPVSIAVAGIVGIAAIYLLIL
                     PWLFGRFIGAADGLRVLLSVVLIAPLAFFMGMPFPLGLRCVAEQAPDFVPWAWGINGF
                     ASVVSAVLATLLAIHFGFTIVIVLAMIIYIAAAALRVA"
BASE COUNT          385 a          815 c          782 g          587 t
ORIGIN      
        1 gcttcgtgcc gctgaccggc aagcactgag ttccgtgcgg cgtctgctgt tagccacgct
       61 gttaatctcc gcggcggcta tcggctacga aatcctgctt atgcgcgtgc tgtcgatcgt
      121 gcagtggcac cacttcgcct acatgatcat cagcctggcg ttgctgggtt atggcgccag
      181 cggacccttc atcgccctgt ttcgccgggt tctcgagccg cgtttcgaac aggccttcgc
      241 aacctgtgcg ctcgccttca gcatcgcgat ggtcgccggt ttcgtggtcg gtcagcgagt
      301 gccgttcaat gcgctcgaga tcgtctggga tcgcaagcag ttactcaacc tggccctgat
      361 gtacctggtt ttcttcgtac cgttcttctt tgccgcctgt tgcgttggtc ttgcgttgac
      421 ctgccgcagc gaggcgatca gccgcctgta tttttgcgat ctcgcgggtg ccggaacggg
      481 agccgtgttg atcatcggcg tgttgctggt cgcgttcccg caatcggcgt tgattgtcct
      541 cgtggcattc gtgctcatcg cctcggtgct cgttggcaac agcaccgctg cgcgagtgcc
      601 gctgagcgtc ggtcaattca cctggctggt cgccctggcg atcatcgttt ttaacggcgg
      661 actgacgttg cgaccctccg agttcaaggg cctcagccag gctttgcagg ttgtcgacgc
      721 ccgtgcgctg acacaagtct ccagtccgct cgggctgctc acggtcgtgg acagtccgac
      781 cgtgccgatt cggcttgcgc cgggcctgag cttcaatacg cgccacgtac cgccggagca
      841 gctcgccgtg ttcaccgacg ccgatggcat gagtgcgata acccaatacg acggcgatcg
      901 cgagtcggtc gccttcctcg cggatgttac ggcggcactg ccgtacgcgt tgctcgagca
      961 accggacgtc ctgattctcg gcgcaggcgg tggcagcgac gtgttattgg ccctctatca
     1021 cggcgcgaat catgtcgatg ccgtcgaact gaatccccgg atgacggaac tcgttgcaga
     1081 cacctacgcc gaattcgcag ggcacctcta cgatgaccct cgcgttgacc tgcacacggc
     1141 agaggcccgc ggctatgtcg cacagaacga tcgacgctac gatctcatcc agatcggcct
     1201 gctcgactcc tttgcggctg ccggcgcggg cgtgcaggcg ctcaacgaaa gctacctgta
     1261 tacggtggag gccgtgggcg agtacctggc cgacctcaat cccggcggag tggtggccat
     1321 cacgcgctgg gtgaagatgc cgccccgtga cagcctgaag ctcgcggcga tcgcgatcga
     1381 ggcactcgag caagcagggg aagtcgatcc cggccgccgg attgcggtca ttcgtagctg
     1441 gaacaccgtg acgctgctca tcaggagggg tgcatttgtg cccagcgaaa tcgacgccat
     1501 gcgcgagttt gcgagttcgc gctcgttcga cacggcctac tttcccggga tcacgagcgc
     1561 cgatgccaat cgcttcaacc tgctggacag gccctggctg tacgatggca tccaggcgat
     1621 gctcggcgat agtgccgacc ggtttttcgc cgactacaaa tactttgtcg aaccggcaac
     1681 cgacgaccgc ccgtacttct ctcatttctt taaatggcgg tccctgcccg aagtgatggc
     1741 gatgcgtgcc tcgggcggtg cgggactcat cgagtgggct tacctcatcg tcgtaacgac
     1801 gctggtgcag gcagccgtca tcgggggcct gttgatcctg ttgccactgt cactcatgaa
     1861 gcgtacctgg gtcggcggta ctcgcttcgg ggcctacttc ctcgcgctcg ggcttgcgtt
     1921 cctgttcgtg gagatcgcgt tcatccagag attcacgctg ttcctgagcc acccgctgta
     1981 tgccgtcgcg gtggtgctcg cgagtttcct cgtgttcgcc ggcgctggca gcgcgtactc
     2041 gctgcgcctg gccgaccgcg ccatcgcccg gaacgtccgg cccgtcagta ttgcggtcgc
     2101 cggcatcgtc ggcatcgcgg cgatctattt gctgatcctg ccgtggctgt tcggccgctt
     2161 catcggcgcc gccgacggcc tgcgagtcct gctctcggtg gtgctgatcg cgccactcgc
     2221 cttcttcatg ggtatgccgt tcccactggg tctcagatgc gtcgcggagc aggcgcctga
     2281 ttttgtgcca tgggcctggg gcatcaatgg tttcgcttcg gtcgtcagcg ctgtactcgc
     2341 aaccctgctg gcgattcact ttggatttac gatcgttatc gtgctcgcga tgatcatcta
     2401 cattgccgcg gcagcgctca gggttgccta gcaccttgcc ggcctcgttt gttactgtac
     2461 ataaatgccg atggcacctg acaagctgga cccgaatgcc gtcgccttat ttctcgatgt
     2521 cgacggcacg ctcctcgaga tcatgaacaa tccggccgac gttagtgcc
//
LOCUS       DATCZV010000397         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_350770, whole genome shotgun sequence.
ACCESSION   DATCZV010000397 DATCZV010000000
VERSION     DATCZV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350770"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VIW27_08925"
     CDS             complement(<1..516)
                     /locus_tag="VIW27_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5739835.1"
                     /translation="MGEKQEVLAHLRTQQDSMTALLKDLVLMETPSTEPAAQAQIRER
                     LKAEFEEIDYRVNLIPGRNSGGHIYASAKYRGRQQPAQLMLGHCDTVWPIGTLQSMPL
                     LMEASTLSGPGVYDMKAGLVEMIYALRTIESLGLRPSVAPLCFINSDEEIGSRESTRY
                     VRALAQRVDRCM"
     gene            complement(525..1133)
                     /locus_tag="VIW27_08930"
     CDS             complement(525..1133)
                     /locus_tag="VIW27_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY5739836.1"
                     /translation="MSDSESTHLIELDISEPIWESFFTVFPLVIVGSREADGRDDLAP
                     KHLAIPMSWQNHFGFVCTPRHNTYQNIQRDKEFAVTYMRPSQTVLASLAASPRCDDGS
                     KPITEAMPTFAAQSVKASFIQDGYLFLECELEQIVDNLGDNSLIIGRIVSARVAEDAL
                     RTSDRDDEDLVYTAPLLAYLYPGRFAEIKNSNKLPFPAGFKR"
     gene            1301..1831
                     /locus_tag="VIW27_08935"
     CDS             1301..1831
                     /locus_tag="VIW27_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEY5739837.1"
                     /translation="MNVLKPRQPVPALEVETLDGPWSLADQSPENFTMVVFYRGLHCP
                     ICTKYIGELDKLASDFADIGVSVLVLSTDTQERAAQARQDWQLSNLNIGYGVSVEQCR
                     DWGLHRSASRGMTSIGIEEPAEFNEPGIFIVRPDNTLYWAQISTMPFARPHFREIIGG
                     LKFALEKDYPARGELS"
     gene            complement(1884..>2558)
                     /locus_tag="VIW27_08940"
     CDS             complement(1884..>2558)
                     /locus_tag="VIW27_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY5739838.1"
                     /translation="ILEFGAAHVAIATGARWRRDGYGRQHQFPIAGTDGDNLLTPDDI
                     MAGTSPRGSVLVYDDDHYYMGSVIAEQLVADGCQVTLVTPDARVASWTDYSLEQHRIQ
                     TRLLELGVDIVTSHELQEITDDSAAVSCVYSGKSRLLAVSSVVMVTSRLPDDSLYQEL
                     AATPDRLRAAGIETFKPIGDCHGPATIAAAVYEGHRFARELGEPVDEIPFRREITELA
                     PGFELP"
BASE COUNT          467 a          757 c          812 g          522 t
ORIGIN      
        1 catgcagcga tcgacgcgct gcgccagcgc gcgcacgtag cgggtggatt cgcggctgcc
       61 gatctcttcg tcggaattga taaagcacag cggcgcgacg ctgggtctga gaccgagcga
      121 ctcgatggtt cgaagcgcgt aaatcatttc cactagcccg gccttcatgt cgtagacccc
      181 gggcccgctc agggtactcg cttccatcag cagcggcatg ctctgcagcg tgccgatcgg
      241 ccataccgtg tcgcaatgcc cgagcattag ttgcgcgggt tgttgccggc cacggtactt
      301 cgccgaggca tagatatgcc cgccggaatt gcgtccgggg atcaggttga cgcggtaatc
      361 gatttcctcg aactcggcct tcaggcgctc gcgtatctgg gcctgggccg cgggctcggt
      421 ggacggcgtt tccattagca ccaggtcctt gagcagcgcc gtcatcgagt cctgctgcgt
      481 gcgcaggtga gccagtactt cctgtttttc gcccatcgtc gggtctacct tttgaagcct
      541 gcgggaaacg gcagcttgtt gctgttcttg atctcggcga aacgtcccgg gtacaggtaa
      601 gccagcaggg gagcggtata gaccaggtct tcgtcgtcgc ggtcagaggt gcgcagcgcg
      661 tcctcggcca cgcgcgcgct gacgatgcgg ccgatgatca gcgaattatc gccgagatta
      721 tcgacgatct gttccagttc gcattcgagg aacaggtaac cgtcctggat gaacgaggcc
      781 ttgaccgatt gcgcggcgaa ggtcggcatg gcctcggtaa tcggtttgct gccgtcgtcg
      841 cagcgcgggc tcgcggccag gcttgcgagc acggtttgcg agggtcgcat gtaggttacg
      901 gcaaactcct tgtcgcgctg aatgttctga taagtgttgt ggcgcggtgt gcagacgaag
      961 ccgaagtgat tctgccagct catcgggatc gcgagatgct tgggcgcgag atcgtcgcgg
     1021 ccatctgcct cgcggctgcc gacgatgacg agcgggaaaa ccgtgaaaaa actttcccag
     1081 atcggctcgc tgatatccag ctcgatcaga tgcgtgcttt cggagtcgga catgccagta
     1141 tcccgctatt gttatcggat ggacattaac atcgacccgt tacgggctcc ttgacgcgta
     1201 tcaactaagc aaggcgggct gatttcgaat ttaaccatgc agatcgagcc ctttttaggt
     1261 atggttcacg cttacaataa atcgacaaat ggagcgtgct gtgaacgtat taaaaccccg
     1321 ccaaccggta ccggccctgg aggtcgaaac tctggacggc ccctggtcgc tggccgatca
     1381 gagtcccgag aatttcacca tggtcgtgtt ctatcgcggg ctgcattgcc cgatctgcac
     1441 caagtatatc ggtgagctgg ataagcttgc ctccgacttt gccgacattg gagtttccgt
     1501 gctcgtgttg agcaccgata cgcaggaacg tgccgcgcag gcgcgacagg actggcagtt
     1561 gagtaatctc aatatcggtt acggcgtcag cgtcgagcag tgccgcgact gggggctgca
     1621 tcgctccgcg agccgcggta tgacatcgat cggcatcgag gaacccgccg agttcaacga
     1681 gcccggcatc ttcatcgtgc gcccggacaa cacgctctac tgggcgcaga tcagcaccat
     1741 gccgttcgcg cgcccgcatt ttcgcgaaat catcggtggc ctcaagttcg ccctcgaaaa
     1801 agactacccc gcgcgcggcg aattgagcta acgcaatcca gatcctgcgt catttccgct
     1861 ggcgcgggga atggcgcgcg gtatcagggc agctcgaagc cgggggcaag ctcggtgatc
     1921 tcgcggcgga acgggatctc gtcgaccggt tcgccgagtt cgcgggcaaa gcggtgaccc
     1981 tcgtaaaccg cggcggcgat ggtcgcgggt ccgtggcaat caccaatcgg cttaaaggtc
     2041 tcgataccgg ccgcgcgcag acgatccggg gtggccgcga gctcctggta caggctgtcg
     2101 tcgggcaagc gcgatgtcac catgaccacc gatgataccg ctagcagccg cgatttaccg
     2161 gagtaaacgc agctcacagc ggcgctgtcg tcggtgattt cctgcagttc gtggctggtg
     2221 acgatgtcga ctccgagctc cagcaggcgc gtctgaattc ggtgctgttc caggctgtag
     2281 tcggtccagg acgcgacccg tgcatccggc gttaccagcg tcacctggca gccgtccgcc
     2341 accagttgct cggcgatgac cgagcccatg tagtagtgat cgtcatcgta aaccagcacg
     2401 ctgccccgcg gactggtgcc ggccatgata tcgtcaggtg tcagcaggtt gtcgccgtcg
     2461 gtgccggcga tcggaaactg gtgttggcgt ccgtagccgt cgcggcgcca gcgcgcgccg
     2521 gtcgcgatgg cgacatgcgc ggcgccgaac tcgagaat
//
LOCUS       DATCZV010000398         2276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_85693, whole genome shotgun sequence.
ACCESSION   DATCZV010000398 DATCZV010000000
VERSION     DATCZV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85693"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VIW27_08945"
     CDS             <1..525
                     /locus_tag="VIW27_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY5739839.1"
                     /translation="MQRLIEACDWRQERITVYGKPYLQPRLSAWHGDLSYRYSGIRLE
                     PRAWTPLLLRLRRCIEAQTGLAFNSLLLNYYRDGMDGMGMHSDDEPELGERPAIASLS
                     LGETRELVLQHRQRRDLETLKLPLPSGSLLLMQGDTQHNWRHGIRKSRRIHGARLNLT
                     FRRVLPGGATTAAV"
     gene            522..947
                     /locus_tag="VIW27_08950"
     CDS             522..947
                     /locus_tag="VIW27_08950"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEY5739840.1"
                     /translation="MTLLSLLIWMLGGLAGSMLFFALVVAPTVFAVLPAEQAGPLLRA
                     LFPRYYLWGLFAALLTTLLALWTDAGGLVISASATVTVLFVFARQNLLPAINRARQRQ
                     LDGDDAAAVRFRRLHAASLIINLAQLLLLATATVWLLRL"
     gene            1004..1354
                     /locus_tag="VIW27_08955"
     CDS             1004..1354
                     /locus_tag="VIW27_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5739841.1"
                     /translation="MTHTELTELSRAFIALCNSHRIDLVLSMFAAGARYTSSASGDYR
                     GRAAIGDMMHVFFAEHPDVYWQADEFRCDDHRVSFDFTMTAGNDTGGIRRQGVEHIDF
                     TADGSIKKITVEVA"
     gene            complement(1364..1945)
                     /locus_tag="VIW27_08960"
     CDS             complement(1364..1945)
                     /locus_tag="VIW27_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEY5739842.1"
                     /translation="MKFIKSVLVLCVLGLLVLVSLAFSSRGDWRSASRASAGIAPDPG
                     TTHEAVLQVYGADAWGWRGWFAIHTWIGAKRKGEKFYTVYDVVGWRGRYGSGVVGIHR
                     DTPDRYWYGSRPEVLKEHRGESAESLIDAVDAAAHLYPWKNTYQVFPGPNSNTFIAWI
                     AQQVPELELDLPFSAIGAGYVDQAAAAGAPGDS"
     gene            complement(2034..>2276)
                     /locus_tag="VIW27_08965"
     CDS             complement(2034..>2276)
                     /locus_tag="VIW27_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY5739843.1"
                     /translation="PRKSAKPYKKALLISSSAAPSWFGRWLFGSQRQLAKTAGIIGAR
                     PVGKLFTGLASKHADQQLPAVSQRRLCRQAERLLAS"
BASE COUNT          386 a          754 c          732 g          404 t
ORIGIN      
        1 atgcagcgcc tgatcgaggc ctgcgactgg cgccaggaac gcatcaccgt ctatggcaag
       61 ccctacctgc agccgcggct cagcgcctgg cacggggacc tgtcttaccg ctattccggc
      121 attcgcctcg agccacgcgc ctggacgccg ctgctactgc ggctgaggcg ctgcatcgag
      181 gcgcaaaccg ggctggcttt caacagcctg ctgctgaatt actaccgcga cggcatggac
      241 ggcatgggaa tgcacagcga cgacgagccc gagctcggcg agcggccggc gatcgcgtcg
      301 ctgagcctcg gcgagacccg cgagctggtc ctgcagcacc gccagcgccg cgacctcgag
      361 acgctgaaac tgccgttacc gtcgggcagc ctgctgctga tgcagggcga tacccagcac
      421 aactggcgtc acgggattcg caaatcgcgg cgcattcacg gcgcgcggct caacctgacc
      481 tttcgcaggg tgctgcccgg cggcgcaacg accgcggccg tatgacgctg ttatcgctac
      541 tcatctggat gctcggcggg ctcgccggca gcatgctgtt ttttgccctg gtcgtggcgc
      601 cgacggtatt tgccgtactg cccgccgagc aggccggacc gttgctgcgt gcgctgtttc
      661 cgcgctatta cctgtggggc ctgttcgctg cgttgctgac cacgctgctg gcgctatgga
      721 cggatgccgg cggactggtg atcagcgcca gcgccacggt cacggtattg ttcgtgtttg
      781 cgcgccagaa cctgctgccg gccatcaacc gcgcgcggca gcgccagctc gacggcgacg
      841 acgccgcggc cgtgcgcttt cggcgactgc acgccgcgag cctgatcatc aacctggcgc
      901 aattgctgct gctggcgact gccacggtct ggctgcttcg gttataattg acaaccgccc
      961 caccgagcct ggggccgttt tcgagcttgc caacgaggtc agcatgacgc ataccgaatt
     1021 gaccgaactc tccagggcct tcatcgcgct gtgcaactcg caccgcatcg acctggtgct
     1081 gtcgatgttc gcggcgggtg cccgttatac gtcgtcggcc agcggcgatt accgcggccg
     1141 cgccgcgatc ggcgacatga tgcacgtctt tttcgccgag catccggacg tttactggca
     1201 ggccgacgaa tttcgctgcg acgatcaccg cgtcagcttc gacttcacga tgaccgccgg
     1261 caacgatacc ggcggcattc gccggcaagg cgtcgaacac atcgatttca ccgccgacgg
     1321 atcgatcaag aagatcaccg tcgaagtcgc ctgaggcgtg cgctcaggag tcgccgggcg
     1381 cgccggcggc cgccgcctgg tcgacatagc cggcaccgat cgcggagaac ggcagatcga
     1441 gttcgagttc cggtacctgc tgcgcgatcc aggcgataaa ggtattgctg ttggggcccg
     1501 ggaaaacctg gtaagtattt ttccacgggt acaggtgggc cgccgcgtcg acggcatcga
     1561 tcagtgactc ggccgattca ccgcggtgct ccttgagcac ctccggccgc gaaccgtacc
     1621 agtaacgatc gggcgtatcg cggtgaatac cgaccacgcc gctgccatag cggccgcgcc
     1681 agccgactac gtcgtaaacc gtgtaaaatt tctcgccctt gcgcttggcg ccgatccagg
     1741 tgtgaatcgc gaaccagccg cgccagcccc aggcgtcggc gccgtagacc tgcagcacgg
     1801 cttcatgggt cgtgccggga tcgggcgcga tccctgccga ggcgcggctg gcgctgcgcc
     1861 agtcgccgcg cgaactgaat gccaggctga ctagtaccaa cagtcccagc acgcaaagca
     1921 cgagcacgga tttaataaac ttcatcgatg gatattttag tcagccggca agaaatgtca
     1981 tcgtcggcgc ggcgcgcgac gatttcgagc ggctgtggaa gctagcgccg gaatcagctc
     2041 gccagcaagc gctcggcctg ccggcacagg cgccgctgac tgacggcggg caattgctga
     2101 tccgcgtgct tcgatgcgag gccggtaaac agcttgccga ccggtctggc accgatgatg
     2161 ccggcggtct tcgccagctg gcgctggctg ccgaacagcc agcggccgaa ccaggagggc
     2221 gccgcgctgg agctgatcag cagggccttc ttgtacggct ttgccgattt acgcgg
//
LOCUS       DATCZV010000399         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_564200, whole genome shotgun sequence.
ACCESSION   DATCZV010000399 DATCZV010000000
VERSION     DATCZV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564200"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VIW27_08970"
     CDS             complement(<1..580)
                     /locus_tag="VIW27_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase TmcA
                     domain-containing protein"
                     /protein_id="HEY5739844.1"
                     /translation="MPANDITRWMTGLLDELEYSRQRQLVALQGTAEWCDTRLAALRE
                     LQPAMLLISNRHTGLEAVAFDNAAACLGGEARLVVLDLFDGFDPDVLCIAGGLVRAGG
                     VLVLMSLPPAEWNTELDRYACWQDGRRSPRAYFVEYFFAELRRQADVGLLVTPRSLPP
                     RPPLPPLQATPRPDGCTAEQARVLEQLQAWLCG"
     gene            631..945
                     /locus_tag="VIW27_08975"
     CDS             631..945
                     /locus_tag="VIW27_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEY5739845.1"
                     /translation="MTKDQQTEAEAAAFRALVEHLQKRTDVQNIDLMNLAGFCRNCLC
                     KWYSAAAKQRDPEFDFSSAQQYVYGMSIEAWKRDFQTEASEEQQRLFAESRPLHAQIT
                     GY"
     gene            complement(1079..>2042)
                     /locus_tag="VIW27_08980"
     CDS             complement(1079..>2042)
                     /locus_tag="VIW27_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5739846.1"
                     /translation="IANMQQASAWIKNVVEDGEEVIITALPLYHIFSLTANCLTFMKV
                     GALNYLITNPRDMPGFVKELQGLRFTAMTGVNTLFNAMMNTPGFERIDFSNLKLALGG
                     GMAVQQAVAERWQKQTGTPLLEAYGLTETSPAVCINPLDLKQFNGSIGLPVPSTEISI
                     QDIDGNMVPLGETGELCVRGPQVMKGYWQRPDETAKVLSADGWLKTGDVARVDEQGYV
                     YIVDRLKDMILMSGFNVFPNEVEAIIASHPGVLEVGVIGQPDEESGEIVKAVIVKKDP
                     ALSEADVVALCRENLTAYKVPKIIEFRDELPKTNVGKILRRELR"
BASE COUNT          413 a          641 c          602 g          386 t
ORIGIN      
        1 gcccgcacaa ccaggcctgc aattgctcga gtacacgggc ctgttccgcg gtgcagccat
       61 cgggtctcgg ggtcgcttgc aggggtggca gcggcggcct tggcggcagc gagcgcggcg
      121 tcaccagcag gccgacatcg gcctgccgcc gcaactcggc gaaaaaatac tcgacgaaat
      181 acgcccgtgg cgaacgcctg ccgtcctgcc aacaggcata gcgatccagc tcggtattcc
      241 actccgccgg cggcagcgac atcagcacca gcacgccacc ggcgcgcacc agtccgccgg
      301 cgatgcaaag cacgtcggga tcgaaaccgt cgaacaggtc gagtaccacc aggcgcgcct
      361 cgccgccgag acaggccgcc gcgttatcga aggcaacggc ttcgagcccg gtgtggcgat
      421 tggagatcag cagcatcgcg ggctgcagtt cgcgcaacgc ggcaagccgc gtgtcacacc
      481 attcagccgt gccctgcagg gccaccagct ggcgttgccg actgtattcg agctcgtcga
      541 gcaagccggt catccagcgg gtgatatcgt ttgccggcat cgaaaatatc tgttaaaaaa
      601 tgcggcccca ttataggtgg atcacgagtc atgaccaaag atcagcaaac cgaggccgaa
      661 gccgcggcat tccgcgccct ggtcgagcac ctgcagaagc gtaccgacgt gcagaatatc
      721 gacctgatga accttgccgg cttttgccgc aactgcctgt gcaagtggta ttcggccgcc
      781 gccaagcaac gcgatcccga gttcgatttc agcagcgccc agcagtacgt ctacggcatg
      841 agtatcgagg cgtggaaacg cgactttcag accgaagcca gcgaagagca gcagcgcctg
      901 ttcgccgaga gcagaccgtt acacgcacag attaccggct actgaaacca gcaggctgca
      961 cgtaatctgg ggaattctga aattcgctct ggcgacagtt ccactgcggc cattccattg
     1021 cgggcatgga caaggcaaaa ctgcgccggg ccgcgccggc tgagtagaat ttcagcggct
     1081 agcgcagttc gcgccgcagt atcttgccga cattggtctt cggcagttcg tcgcgaaatt
     1141 cgatgatctt cggtaccttg taagcggtca ggttttcgcg gcaaagggcg acgacgtccg
     1201 cttcgctaag cgcgggatcc ttcttcacga tcactgcctt gacgatctcg ccgctttctt
     1261 cgtcgggctg gccgatgacg ccgacctcga gcacgcccgg gtggctggcg atgatcgcct
     1321 cgacttcgtt ggggaacacg ttgaagcccg acatcaggat catgtccttt aaacgatcga
     1381 cgatatacac gtatccctgt tcgtcgaccc gggcaacgtc gccggtcttc agccagccat
     1441 cggcggacaa gaccttggct gtctcatcgg gccgctgcca gtaacccttc atgacctgcg
     1501 ggccacgcac gcacagctcg ccggtttcgc ctagcggcac catgttgccg tcgatgtcct
     1561 ggatcgaaat ctcggttgac ggcaccggca agccgatgga accattgaac tgtttcagat
     1621 cgagcggatt gatacacacc gcgggcgaag tttcggtgag cccgtaggct tcgagcaacg
     1681 gcgtgccggt ctgtttttgc cagcgctcgg cgactgcctg ctgcactgcc ataccgccgc
     1741 cgagagccag tttcaggttg gagaaatcga tcctctcgaa tcccggcgta ttcatcatag
     1801 cgttgaacag cgtgttgacc ccggtcatcg cggtaaaccg caggccctgc aattccttca
     1861 cgaatcccgg catatcgcgc gggttggtga tcaggtagtt cagggccccc accttcatga
     1921 aggtcaggca attagcggtc agcgaaaaaa tatggtaaag cggcagcgcg gtgatgatca
     1981 cttcctcgcc gtcctcgacc acgttcttga tccatgccga ggcctgctgc atgttggcga
     2041 tc
//
LOCUS       DATCZV010000400        10871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_192775, whole genome shotgun sequence.
ACCESSION   DATCZV010000400 DATCZV010000000
VERSION     DATCZV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192775"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            793..1425
                     /gene="rplC"
                     /locus_tag="VIW27_08985"
     CDS             793..1425
                     /gene="rplC"
                     /locus_tag="VIW27_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY5739847.1"
                     /translation="MSLGLVGRKVGMTRVFSDDGVSIPVTVIEIEPNRVSQIKTVDTD
                     GYVAVQVITGTRRASRVTKPMAGHYKKAGVEPGRGAWEFRVDSLDGIELGSEFKVDLF
                     EAGQIVDICGVSKGKGFQGGVKRHNFHMQDATHGNSLSHRANGSIGQNQTPGRVFKGK
                     KMSGHMGAANVTTQNLEIVTVDTDRNLLLIRGAVPGAKSGDVIVRPASKR"
     gene            1461..2066
                     /gene="rplD"
                     /locus_tag="VIW27_08990"
     CDS             1461..2066
                     /gene="rplD"
                     /locus_tag="VIW27_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY5739848.1"
                     /translation="MDITIHNSNKKVSVSDAAFAASFNETLVHQLVVSYLAGARAGTR
                     AQKNRSAVRGGGAKPWRQKGTGRARAGTIRSPLWRSGGVTFAATPRDYSKKLNKKMYR
                     VGMRSLVSELLRQDRLIVVDKLGVTEPKTKQMQARLKELGVDNALLLTDGLDHAVYLA
                     ARNIPDIQVMDIAIVDPVSLISPQKVVIDEAALKKLEERLS"
     gene            2063..2362
                     /gene="rplW"
                     /locus_tag="VIW27_08995"
     CDS             2063..2362
                     /gene="rplW"
                     /locus_tag="VIW27_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY5739849.1"
                     /translation="MNPERIFQILLTPHISEKSAVLGDASGQAVFQVARDAKKAEIKA
                     AVEQLFDVKVANVRTVNMKGKSKRMGLRRGRRSDWKKAYVSLEQGHEIDLASIGG"
     gene            2368..3198
                     /gene="rplB"
                     /locus_tag="VIW27_09000"
     CDS             2368..3198
                     /gene="rplB"
                     /locus_tag="VIW27_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY5739850.1"
                     /translation="MALIKAKPTSPGRRFVVSVKTPGLHKGKPHAALLDKKAKTGGRN
                     NAGRITSRHTGGGHKQRYRMIDFKRNKDGIDAVVERLEYDPNRSAHIALLKYADGEWR
                     YIIAPKGIKAGDRLRSGDDAGIRVGNCLPLRNIPVGSVVHCIELKPGKGAQMARSAGT
                     SAQLVAREGQYATLRLRSGEMRKVLSDCRASIGEVGNGEHSLRSLGKAGASRWRGNRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGTPTKGYKTRSNKRTDHLIVRRRSKRK
                     "
     gene            3202..3480
                     /gene="rpsS"
                     /locus_tag="VIW27_09005"
     CDS             3202..3480
                     /gene="rpsS"
                     /locus_tag="VIW27_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY5739851.1"
                     /translation="MPRSLRKGPFIDQHLMQKVLTAVQTNNKRPIKTWSRRSMIVPDM
                     VGLTIAIHNGRQHVPVLINENMVGHKLGEFSQTRTYRGHIVDKKAKKR"
     gene            3488..3820
                     /gene="rplV"
                     /locus_tag="VIW27_09010"
     CDS             3488..3820
                     /gene="rplV"
                     /locus_tag="VIW27_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY5739852.1"
                     /translation="METSARHRHAKISPQKCRLVADQVRGMPVEKALTLLTFSNKKAA
                     MLVRKVLESAIANAEHNDGADIDELKVASIYVDQGPVMKRMRARAKGRGNRILKRTSH
                     ITVTVADS"
     gene            3831..4508
                     /gene="rpsC"
                     /locus_tag="VIW27_09015"
     CDS             3831..4508
                     /gene="rpsC"
                     /locus_tag="VIW27_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY5739853.1"
                     /translation="MGQKVHPTGFRLGIATDWTSKWYADRGQFADFLDEDLKIRDFLK
                     KKLAQAAVSRIQISRPAKAIAVTIHTARPGIIIGKKGEDIEKLRIEVAQLVNIHINNV
                     KINIEEIRKPELDAQLVAEGIASQLERRVMFRRAMRRSVTNAMRIGAEGIKINVAGRL
                     NGAEIARSEWYREGRVPLHTLRADVDYGFAEALTTYGILGVKVWIYKGEVFDLENKDA
                     AASQASK"
     gene            4521..4934
                     /gene="rplP"
                     /locus_tag="VIW27_09020"
     CDS             4521..4934
                     /gene="rplP"
                     /locus_tag="VIW27_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY5739854.1"
                     /translation="MLQPKRTKFRKQFKGRNRGLATTGNRVSFGEYGLKAVERGKMTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDVPVSKKPLEVRMGKGKGNVEYWVCKIQPGRV
                     LYEMEGVNESIAREAFRLAAAKLPFKTTFVSRTVM"
     gene            4934..5137
                     /gene="rpmC"
                     /locus_tag="VIW27_09025"
     CDS             4934..5137
                     /gene="rpmC"
                     /locus_tag="VIW27_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY5739855.1"
                     /translation="MKAADLRAKTEQELRQELLELRREQFNLRMQRGINPDAVRNNKI
                     AEVRKDIARVKTVINENRSAKSS"
     gene            5134..5388
                     /gene="rpsQ"
                     /locus_tag="VIW27_09030"
     CDS             5134..5388
                     /gene="rpsQ"
                     /locus_tag="VIW27_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY5739856.1"
                     /translation="MNEKVLRIQTGSVVSDKMDKSAVVLIERRVKHPVYGKFVKKSTK
                     LHIHDENNECGIGDTVQITECRPISKTKSWRLVKVIEKAN"
     gene            5409..5777
                     /gene="rplN"
                     /locus_tag="VIW27_09035"
     CDS             5409..5777
                     /gene="rplN"
                     /locus_tag="VIW27_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY5739857.1"
                     /translation="MIQMQTVLNAADNSGARKMQCIKVLGGSKRRYAAIGDIIKVSIK
                     DAIPRGRVKKGEVYNAVVVRTAHGVRRPDGSAIKFDGNAAVILNNNLQPIGTRIFGPV
                     TRELRGEKFMKIISLAPEVL"
     gene            5789..6106
                     /gene="rplX"
                     /locus_tag="VIW27_09040"
     CDS             5789..6106
                     /gene="rplX"
                     /locus_tag="VIW27_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY5739858.1"
                     /translation="MQKIRKGDEVIVIAGRSKGQRGNVLKRLEDGRLLVENVNMVKKH
                     VKGNPRSGEAGGITDQEAPIDISNVMLFNPHTQKGDRVGFKTLEDNRKVRYFKSNGEV
                     VDI"
     gene            6116..6655
                     /gene="rplE"
                     /locus_tag="VIW27_09045"
     CDS             6116..6655
                     /gene="rplE"
                     /locus_tag="VIW27_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY5739859.1"
                     /translation="MTRLEQYYRDTVAAQLQEQFSYQSPMQIPRITKITLNMGVGEAA
                     QDKKVIEHAAEDLALIAGQKPVITVAKKAIAGFKIREGMTIGCKVTLRRDKMYEFLDR
                     LIHFAIPRIRDFRGLSPRAFDGQGNYNLGMSEQISFPEIDYDRIDKLRGLNISITTSA
                     CSDEEGRALLAAFQFPFRG"
     gene            6669..6974
                     /gene="rpsN"
                     /locus_tag="VIW27_09050"
     CDS             6669..6974
                     /gene="rpsN"
                     /locus_tag="VIW27_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY5739860.1"
                     /translation="MAKNSMVQRELKRSRLAAKYADKRSELKAIIKSADAGYDEKMAA
                     VEQLQKLPRDSSPVRKQNRCRVTGRPHGVYRKFGLCRNKIREAAMRGDIPGLVKASW"
     gene            6985..7377
                     /gene="rpsH"
                     /locus_tag="VIW27_09055"
     CDS             6985..7377
                     /gene="rpsH"
                     /locus_tag="VIW27_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY5739861.1"
                     /translation="MSLQDPISDMLTRVRNAQKAKKVSVRMPSSKQKVRIAAVLKDEG
                     YISDFKVTDDKLKQLVIELKYYQGKPVIEEMRRISRPGLRIFKSKDELPSVNGGLGIA
                     IISTSKGVMTDSQARAAGHGGEVICSVI"
     gene            7388..7921
                     /gene="rplF"
                     /locus_tag="VIW27_09060"
     CDS             7388..7921
                     /gene="rplF"
                     /locus_tag="VIW27_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY5739862.1"
                     /translation="MSRIAKQPIELPNGVEFKIDGREVSAKGPKGQLSLTLHDAVQVV
                     QEDNVLRLSPKDGRQSSLAVTGTMRSVISNIVQGVANGFEKRMQLVGVGYRAQAQGKQ
                     LNLSVGYSHPVNYAVPDGIEIETPVATEIVVRGCDKHLVGQVCAEIRAYRPPEPYKGK
                     GIKYADETIVRKEAKKK"
     gene            7925..8278
                     /gene="rplR"
                     /locus_tag="VIW27_09065"
     CDS             7925..8278
                     /gene="rplR"
                     /locus_tag="VIW27_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011078817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY5739863.1"
                     /translation="MDKKNSRIRRSRRARAKIAELGVDRLSVHRTPRHTYAQVFSGET
                     GKVVATASTLSAEVRKSVKYPGNVEAAAVVGKLIAERARAAGVETVAFDRSGFKYHGR
                     VKALADSAREHGLKF"
     gene            8302..8808
                     /gene="rpsE"
                     /locus_tag="VIW27_09070"
     CDS             8302..8808
                     /gene="rpsE"
                     /locus_tag="VIW27_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY5739864.1"
                     /translation="MAEPRNSQSGDDLMEKLVAVNRVAKVVKGGRQFGFTALTVVGDG
                     NGKVGFGYGKAREVPLAIQKAMEKARSNMISVSITNGTLQYPLNARHGAAKVYMQPAS
                     EGTGVIAGGAMRAVFEAVGVHNVLAKCNGSRNPINLVRATINGLKQMKAPEQIAAKRG
                     KTVEEITG"
     gene            9092..9265
                     /gene="rpmD"
                     /locus_tag="VIW27_09075"
     CDS             9092..9265
                     /gene="rpmD"
                     /locus_tag="VIW27_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY5739865.1"
                     /translation="MVKLTLVRSKYGRLKSHQACIAGLGLRRMHQTVQVIDTPENRGM
                     INKVQYMLKIEEA"
     gene            9265..9699
                     /gene="rplO"
                     /locus_tag="VIW27_09080"
     CDS             9265..9699
                     /gene="rplO"
                     /locus_tag="VIW27_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY5739866.1"
                     /translation="MYLNSLKPAKGSNKSSKRVGRGIGSGHGKTAGRGHKGQKSRAGG
                     GVPARFEGGQMPLQMRLPKFGFTSRKSRYVAEVRLGEIEIAGLDEVSLVKLRETGLVP
                     AQTRKAKIILSGELKRKVSVDASVAVTRGARAAIEAAGGKVA"
     gene            9707..>10871
                     /gene="secY"
                     /locus_tag="VIW27_09085"
     CDS             9707..>10871
                     /gene="secY"
                     /locus_tag="VIW27_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5739867.1"
                     /translation="MAGTSAQQIASSLSGGSLRELRKRIFFVIIALVIFRIGTFIPVP
                     GIDINVMREIFEQQRTGILGMFNMFSGGALERMSIFALGVMPYISASIIMQLMTVAVP
                     YLEQLKKEGESGRRKITQYTRYGTVVLATFQSIGISIALSGQSGGGGAALVVNPGPSF
                     IFTAAVTLVTGTMFLMWLGEQITERGIGNGISILIFAGIVAGLPSAIGGTFELVRTGE
                     LNSLVILALFVGVLAVTYFVVFVERGQRRITVNYAKRQQGRKMYAAQSSHLPLKLNMA
                     GVIPPIFASSIILFPATLGSWFGQAEGMRWLQDISATLSPGQPLYVMFYASAIIFFCF
                     FYTALVFNSKDTAENLKKSGAFIPGIRPGNQTANYIDGVMSRLTLVGAIYITAV"
BASE COUNT         2434 a         3189 c         3408 g         1840 t
ORIGIN      
        1 tccggcatcc gccggaaacg acgagaattg tcatcccgga cgccgcgcag cggcgatccg
       61 ggatcttgta acttttccaa ggtatcatcc agtcatcccg gacgccgcgc agcggcgatc
      121 cgggatcttg taacttttcc aaggtatcat ccagtcatcc cggacgccca gcaggggcta
      181 gcgataggtc ggaccgccgg gatctggtaa cgattgcgcg atcgctattt tagtcaccct
      241 gaaaccgcgt agcggttatc ggggatctcg taatggcgcc gtggtaccag acccccgctt
      301 gcgcggggat gaccggaaag cagcaagatc cccgcttgcg cggggatgac cggaaagtag
      361 caggatcccc gcttgcgcgg ggatgaccgg aaagtagcaa gatccccgct tgcgcgggga
      421 tgaccggaaa gtagcaggat ccctgcttgc gcgggcacgg cgcggcccgc actctcgttg
      481 gcggaatccc gcaacgagaa ggcaattttt aagccaaaaa cgcttacatt tgcgcggctt
      541 atctgctata gtgcgcgccc tcgcggctcg atacgggact tctgtatcgg gccgcttgtt
      601 cgaaaacagg gcagtaattc atgccgcgag gcacgagcga aacccgccgc gaacggaagc
      661 gcggtcgttt tccctggcga tcaagtcatt gaaaacgaaa gaaaaataca gagggcttgt
      721 atttttcgtc atttgaaaat ccggggacgg attaatcaaa tgcgacagct aacttttttt
      781 gaggtttaac aaatgtctct cggtctggta ggacggaaag tcggtatgac ccgggttttc
      841 tcggacgatg gtgtctcgat cccggttacg gtcatcgaaa tcgagcccaa tcgggtatcg
      901 cagatcaaaa ccgtcgacac cgacggctac gtggctgtgc aggtgatcac cggcacccgg
      961 cgcgccagcc gcgtgaccaa accgatggcg ggtcactaca agaaggcggg cgtcgagccg
     1021 ggccgcggcg cgtgggaatt ccgcgtcgat tcgctcgacg gtatcgagct cggcagcgaa
     1081 ttcaaggtcg acctgtttga agccggccaa atcgtcgaca tttgcggcgt ttccaagggc
     1141 aagggctttc aagggggcgt caagcgccac aatttccata tgcaggacgc tacccacggc
     1201 aactcgctgt cgcaccgcgc caacggctcg atcggccaga accagactcc tgggcgggtc
     1261 ttcaagggca agaagatgtc cggccacatg ggtgcggcca acgtgaccac gcaaaacctc
     1321 gaaatcgtca ctgtcgacac cgaccgcaac ctgctgttga tcagaggcgc ggttccgggc
     1381 gccaagagcg gcgacgtgat cgttcgcccg gcgtccaagc gataagatcg gtaactgcag
     1441 acagatagag atttcagctc atggatatca ccatccacaa ctcgaacaaa aaggtatcgg
     1501 tttccgacgc cgcgtttgcc gccagcttca atgaaacgct ggtgcaccag ctggtcgtat
     1561 cctacctggc cggcgcgcgc gccggcacca gggcgcaaaa gaatcgcagc gcggtgcgcg
     1621 gcggtggcgc caagccgtgg cgccagaagg gcaccggccg cgcacgcgcg ggcaccattc
     1681 gcagcccgct gtggcgttcg ggcggggtga ctttcgccgc cacgccgcgc gattacagca
     1741 agaagctgaa caagaagatg taccgcgtcg gcatgcgcag cctggtttcc gagctgctgc
     1801 gccaggaccg actgatcgtg gtcgacaagc tgggtgtgac cgagccgaaa accaaacaga
     1861 tgcaggcgcg tctcaaggag ctgggtgtcg acaatgcgct gctgctgacc gacggcctcg
     1921 atcacgcggt gtatctcgcg gcacgcaata ttcccgatat ccaggtgatg gatatcgcca
     1981 ttgtcgatcc ggttagcctg atcagcccgc aaaaggtcgt aatcgacgaa gccgcgctga
     2041 agaaactcga ggagcgcctg tcatgaatcc cgaaagaatc ttccagatcc tgctgacccc
     2101 gcacatttcg gagaaatccg cggtgctcgg tgatgcctcc ggccaggcgg tgttccaggt
     2161 cgccagggac gccaagaaag cggagatcaa ggccgccgtc gagcagttgt tcgacgtcaa
     2221 ggtagccaat gtgcgcaccg tcaacatgaa gggcaagagc aagcgcatgg gcttgcgccg
     2281 tggccggcgt tccgactgga aaaaagcata tgtttcactc gagcagggtc atgaaatcga
     2341 cctcgcatcc ataggcggtt aagcatcatg gcattgatca aggcaaaacc cacttctccc
     2401 ggacgccgct tcgtcgtctc ggtcaagacc ccgggtctgc acaagggcaa gccccacgcg
     2461 gcgctgctcg acaagaaggc gaagaccggc gggcgcaaca acgccgggcg tatcacctcg
     2521 cgtcataccg gcggcgggca caagcagcgc taccgaatga tcgacttcaa gcgcaacaag
     2581 gacggcatcg atgccgtggt cgagcgcctc gagtacgatc ccaaccgcag tgcgcatatc
     2641 gcgctgctga aatacgccga tggcgagtgg cgctatatca tcgcccccaa gggtatcaag
     2701 gccggcgacc ggctgcgctc cggcgacgat gccggtatca gggtcggcaa ttgcctgcca
     2761 ctgcgcaata tcccggtcgg ctcggtggtg cactgcatcg aattgaagcc tggcaagggc
     2821 gcgcaaatgg cgcgttcggc cggcacctcg gcacagctgg tggcacgcga aggccagtac
     2881 gccacgctgc gcctgcgctc cggtgaaatg cgcaaggtgc tttccgattg ccgcgcgtcg
     2941 atcggcgagg tcggcaacgg cgagcacagc ctgcgctcgc tgggcaaggc cggcgcatcg
     3001 cgctggcgcg gcaaccgccc gacggtccgc ggtgtggcga tgaacccggt cgatcacccg
     3061 cacggcggcg gcgagggccg tacctcgggt ggccgccacc cggtctcgcc gtggggaacc
     3121 ccgaccaagg gatacaagac acgttcgaac aagcgcaccg accacctgat cgtgcgtcgt
     3181 cggagcaaga ggaaataaca gatgccacgt tcactgagaa aaggcccgtt tatcgatcag
     3241 cacctgatgc aaaaagtgct gactgcggtg cagaccaaca acaagcgccc gatcaagacc
     3301 tggtcgcggc gctcgatgat cgtgcccgat atggtcggtc tgacgattgc gatccacaac
     3361 ggccgccagc acgtgccggt gttgatcaac gagaacatgg tcggccacaa gctcggcgag
     3421 ttttcgcaga cgcgcactta ccgcggccac atcgtcgaca agaaagcgaa gaaaaggtag
     3481 ggattcaatg gaaaccagtg ccagacatcg tcacgccaag atttcgccgc agaagtgccg
     3541 cctggtcgcc gaccaggtgc gtggcatgcc ggtcgagaaa gccctgaccc tgctgacctt
     3601 cagtaacaag aaggccgcca tgctggtgcg caaggtgctc gagtcggcga tcgccaatgc
     3661 cgagcacaac gacggcgccg atatcgacga actcaaggtg gcgtcgatct acgtcgacca
     3721 ggggccggtg atgaagcgca tgcgcgcgcg cgccaagggt cgtggcaatc gaattttaaa
     3781 gcgtaccagt cacattaccg tgactgtcgc ggatagttaa agaggccagc atgggacaaa
     3841 aagtacatcc aacgggtttt cgtctcggta tcgctaccga ctggacctcg aagtggtatg
     3901 ccgaccgtgg tcagttcgcg gattttctcg acgaggacct gaagattcgc gacttcctga
     3961 agaagaagct ggcgcaggcc gcggtcagcc gcatccagat ttcgcgtccg gcgaaggcga
     4021 tcgcggtgac catccatacc gcacgccccg gcatcatcat cggcaagaag ggcgaggata
     4081 tcgaaaagct gcgcatcgaa gtcgcgcagc tggtgaatat ccacatcaac aacgtcaaga
     4141 tcaacatcga ggaaatccgc aagccggaac tcgatgcaca gctggtcgcc gagggcatcg
     4201 cctcgcagct ggagcgtcgc gtgatgtttc gccgcgcgat gcgccgctcg gtaaccaatg
     4261 cgatgcgcat cggcgccgag ggcatcaaga tcaacgtcgc cggccgcctc aacggcgccg
     4321 agatcgcgcg tagcgagtgg taccgcgagg gtcgtgtgcc gctgcacacg ctgcgcgccg
     4381 acgtcgatta cggttttgcc gaggcattga cgacctacgg catcctcggc gtcaaggtgt
     4441 ggatttacaa gggcgaggtg ttcgatctcg agaacaagga cgccgcggcc tcgcaggcat
     4501 cgaagtaggg taagcgagtt atgttacagc ctaaacgaac caagtttcgc aagcagttca
     4561 agggtcgcaa tcgcggcctg gcgacgaccg gcaaccgggt cagcttcggc gaatacggcc
     4621 tcaaggcggt cgagcgcggc aagatgaccg cgcgccagat agaggccgcg cgccgcgcga
     4681 tgacccgcca catcaagcgt ggcggcaaga tctggattcg cgtgtttccg gacgtgccgg
     4741 tttccaagaa gccgctggaa gtgcgcatgg gcaagggcaa gggcaacgtc gagtactggg
     4801 tgtgcaagat tcaacccggt cgtgtgctct atgaaatgga aggcgtcaac gaaagcatcg
     4861 cgcgcgaggc gtttcgcctg gccgccgcca agctgccctt caagacaacc ttcgtttcca
     4921 ggacggtgat gtgatgaaag ctgcagattt acgtgccaag acggagcagg aactgcgcca
     4981 ggaactgctc gagctgcggc gcgagcaatt caacctgcgc atgcagcgcg gcatcaatcc
     5041 ggatgcggtg cgcaacaaca agattgccga ggtgcgcaag gatatcgcgc gcgtcaaaac
     5101 cgtgatcaac gagaatcgga gtgcaaaatc gtcatgaatg aaaaagtcct gaggatccag
     5161 accggttccg tggtcagcga caagatggac aagtcggccg tggtgctgat cgagcgtcgt
     5221 gtcaagcacc cggtttacgg caagttcgtg aaaaagtcga ccaagttgca tattcacgac
     5281 gaaaacaacg agtgcggtat cggtgatacc gtgcagatta ccgagtgccg gccgatctcc
     5341 aagaccaagt cctggcggtt ggtcaaggtc atcgagaaag ccaactagga acgcgcggga
     5401 agacagtcat gatacagatg caaacagtac tcaacgccgc ggacaacagc ggtgcgcgca
     5461 agatgcagtg catcaaggtg ctcggcggct cgaagcgccg ctacgccgcg atcggcgaca
     5521 tcatcaaggt cagcatcaag gatgcgatcc cgcgcggccg ggtcaagaag ggcgaggtct
     5581 acaatgccgt cgtcgtgcgc accgcccacg gcgtgcgccg tcccgacggc tcggcgatca
     5641 agttcgacgg caacgcagca gtcattctca acaataacct gcagccgatc ggtacccgta
     5701 tcttcgggcc ggtcacgcgt gaactgcgcg gcgaaaagtt catgaagatc atttcgctgg
     5761 cgccggaagt actttaggta acgcaaacat gcaaaagatt agaaaaggtg acgaagtcat
     5821 cgtgatcgcg ggtcgcagca agggccagcg cggcaacgtg ctcaagcgtc tcgaggacgg
     5881 ccgcctgctg gtggaaaacg tcaacatggt gaagaagcac gtcaagggca acccgcgcag
     5941 cggcgaagcc ggtggtatca ccgaccagga agcgccgatc gacatttcca acgtgatgct
     6001 gttcaacccg catacccaga agggtgaccg ggtcggcttc aagactctcg aagacaatcg
     6061 caaggttcgc tacttcaagt ccaacggcga agtggtggat atttaggatc gagttatgac
     6121 cagactagag caatattatc gcgatacggt cgccgcgcag ctgcaggagc aattctccta
     6181 ccagtcgccg atgcagatac cgaggatcac caagatcacc ctcaacatgg gtgtcggcga
     6241 agccgcgcag gacaagaaag tcatcgagca tgcggccgag gatctggcgc tgatcgccgg
     6301 ccagaagccg gtcattaccg tcgccaagaa ggccatcgcg ggcttcaaga ttcgcgaggg
     6361 catgaccatc ggctgcaagg tcacgctgcg ccgcgacaag atgtacgagt tcctcgaccg
     6421 cctgatccat ttcgcgattc cgcggattcg cgactttcgg ggcctgagcc cgcgggcctt
     6481 tgacggccag ggcaactaca acctgggcat gagcgagcag atttcgtttc cggaaatcga
     6541 ttacgaccgc atcgacaagc tgcgtggact gaacatcagc attaccacca gcgcatgcag
     6601 cgacgaggaa ggccgcgcac tgctggcggc attccagttt cctttcaggg gatagacgag
     6661 acaagattat ggcaaagaat tccatggtac agcgcgagtt gaagcgtagc cgcctggcgg
     6721 cgaaatacgc cgacaagcgc agcgagttga aggcgatcat caagagcgcc gacgccggct
     6781 acgacgaaaa aatggccgcc gtcgagcaat tgcagaaatt gccgcgcgac tcgagcccgg
     6841 tacgcaagca gaaccgctgc cgtgtcaccg gacgacccca tggcgtttac cgtaaattcg
     6901 gcctgtgccg caacaagatc cgcgaagccg cgatgcgcgg cgatattccg ggtctcgtca
     6961 aggccagctg gtaagaggaa agaaatgagc ttacaagatc cgatctcaga tatgctgacc
     7021 cgcgtgcgca atgcgcaaaa ggcgaagaag gtcagcgtcc gcatgccgtc gtccaagcaa
     7081 aaggtcagaa tcgccgccgt gctcaaggac gaaggttata tcagtgattt caaggttacg
     7141 gacgacaagc tgaagcagct ggtcatcgag ctgaagtatt accagggcaa gccggtcatc
     7201 gaggagatgc gccgtatcag ccgcccgggc ctcagaatct tcaagtcgaa ggacgagttg
     7261 ccgtcggtca acggcgggct cgggatcgcg atcatttcga cttccaaggg agtcatgacc
     7321 gattcccagg cgcgcgccgc gggacacggc ggcgaagtga tctgttcggt tatctaggaa
     7381 taacgaaatg tcgagaattg caaaacagcc catcgagttg ccgaacggcg tcgagttcaa
     7441 gatcgacggc cgcgaggtat cggccaaggg cccgaagggg cagttgtcgt taacgctgca
     7501 cgatgccgtg caggtggtgc aggaagacaa cgtgctgagg ctgtcgccga aagacggtcg
     7561 tcaatcgtcg ctggcggtca ccggcaccat gcgctcggta atcagcaaca tcgtgcaggg
     7621 tgtcgccaat ggtttcgaaa agcgcatgca gctggtcggt gtcggctacc gcgcgcaggc
     7681 ccagggcaag cagctcaacc tgtcggtggg ctactcgcac ccggtaaact acgcggtacc
     7741 ggacggtatc gagatcgaga cgccggtggc gaccgagatc gtggtgcggg gttgcgacaa
     7801 gcacctcgtc ggccaggtgt gcgcggaaat ccgcgcctat cgccccccgg aaccctacaa
     7861 gggcaagggc atcaagtacg cggacgagac tatcgttcgc aaagaagcga agaagaagta
     7921 ggctatggac aagaaaaatt ccagaatccg gcgttcgcgc cgcgcgcgcg ccaagatcgc
     7981 ggagctcggt gtcgatcgcc tgagtgtgca ccgcacgccg cgtcatacgt acgcgcaggt
     8041 attttccggt gagaccggca aggtggttgc cacggcatcg accctgtccg cggaagttcg
     8101 caagtcagtc aagtacccgg gcaacgtcga agcggcagcc gtggtcggca agctgatcgc
     8161 cgaaagagca cgagccgccg gtgtcgagac ggtcgccttc gatcgttcgg gattcaagta
     8221 ccacggcagg gtcaaggcgc tggccgactc cgcgcgtgaa catggtttga aattttaagc
     8281 gcggttaagc gggagaaaga catggcagaa cccagaaact cgcagtccgg cgacgacctg
     8341 atggaaaaac tggtcgcggt gaatcgcgtc gccaaggtcg tcaagggcgg ccgccagttc
     8401 ggttttaccg cgctgaccgt ggtcggcgac ggcaatggca aggtcggctt cggctacggc
     8461 aaggcgcgcg aagtgccgct ggcgattcag aaggcgatgg aaaaggcgcg ctccaacatg
     8521 atcagcgtgt cgatcaccaa cggcaccctg cagtacccgc tgaatgcccg ccacggcgcg
     8581 gccaaggtct acatgcagcc ggcctcggaa ggtaccggcg tcatcgccgg cggcgcgatg
     8641 cgcgcggtgt tcgaggcggt cggtgtacac aacgtgctcg ccaagtgcaa cggttcgcgc
     8701 aacccgatca acctggtgcg cgcgaccatc aacggcctca agcaaatgaa ggcgcccgag
     8761 cagatcgcgg ccaagcgcgg caagaccgtc gaggaaatta caggatagcg tgatggctga
     8821 agacgaagac acagttaaga aagcggccaa caaaaaggcg tcggccaaga aggcggcgac
     8881 caagaaggcg gcgaccaaga aggcggtcgc caaaaaggcc gcgccgaaga aagccgtagc
     8941 gagcaaggcg gccgccgata aagccgcgcc cgccgaacag gcggtcgaag cgaagccggc
     9001 cgcgccgaag ccggctgcgc cgaagccggc cgcgccgaag ccggccgcgc cgaaaaaagc
     9061 tgcggcacca aaaaccgaag cgggagtcaa aatggtcaag ctgacgctgg tacgcagcaa
     9121 gtacgggcgg ctgaagtcgc atcaggcctg cattgccggc ctcgggttac ggcgcatgca
     9181 ccagaccgtg caggtgatcg atacgcccga aaaccgcggc atgatcaaca aggttcaata
     9241 catgctgaag atcgaggaag cgtgatgtac ttgaatagcc tcaagcccgc caagggctcc
     9301 aacaagtctt ccaagcgggt cggccgtggg attggttccg ggcacggcaa aaccgcgggt
     9361 cgcggccata agggacaaaa gtcacgcgcc ggtggcggcg tgccggcgcg cttcgagggc
     9421 ggccagatgc cgctgcagat gcgcctgccg aagttcggtt tcacctcgcg caaatcgcgc
     9481 tacgtcgccg aggtgcgact cggtgaaatc gaaatcgccg ggctcgacga ggtcagcctg
     9541 gtgaaattgc gcgagaccgg gctggttcct gcacagaccc gcaaggcgaa gatcatcctc
     9601 tcgggagaac tcaagcgcaa ggttagcgtc gacgccagcg tcgccgtgac tcgtggcgcg
     9661 cgggcggcga tcgaagccgc cggcggcaag gtagcgtagg cgaatcgtgg ccgggacttc
     9721 agcacaacaa atcgccagca gcctctcggg aggctcgctg cgcgaactgc gcaagcgcat
     9781 attcttcgtc atcattgcgc tggtcatttt ccgcatcggc accttcatcc cggtgcccgg
     9841 aatcgacatc aacgtgatgc gcgaaatctt cgagcagcag cgcaccggta ttctcggcat
     9901 gttcaacatg ttctccggcg gcgcgctcga gcgcatgtcg attttcgcgc tcggcgtcat
     9961 gccgtacatc tcggcgtcga tcatcatgca gttgatgacc gtcgcggtgc cttacctcga
    10021 gcaattgaag aaagaaggcg agtccggccg gcgcaagatc acccagtaca cgcgctacgg
    10081 cactgtcgta ctggcgactt tccagtcgat cggcatttcg atcgcgttgt ccggccagag
    10141 cggcggcggc ggagcggcgc tggtggtcaa tcccggcccg tcgtttattt tcaccgcggc
    10201 cgttaccctg gttaccggca ccatgttcct gatgtggctc ggcgagcaga tcaccgagcg
    10261 cggcatcggc aacggtatct cgatcctgat tttcgccggt atcgtcgccg gcctgccgtc
    10321 ggcgatcggc ggcaccttcg aactggtgcg caccggcgaa ctgaattcgc tggtgatcct
    10381 cgcgctgttc gtgggcgtgc tcgcggtcac ttatttcgtg gttttcgtgg aacgcggcca
    10441 aaggcgcatc accgtcaact atgccaagcg gcagcagggg cgcaagatgt acgccgcgca
    10501 aagcagtcac ctgccgctga agctgaacat ggccggcgtt attccgccga tcttcgcctc
    10561 cagtatcatt ctgttcccgg ccacgctcgg cagctggttc ggacaggccg agggtatgcg
    10621 ctggctgcag gatatttccg cgaccctgtc gcccggacag ccgctgtacg tcatgtttta
    10681 cgcctcggcg atcattttct tctgcttctt ttataccgcg ctggtgttca actccaagga
    10741 taccgcggag aacctgaaga aatcgggcgc ctttattccg ggtattcgcc cgggcaacca
    10801 gacggcgaat tacatcgacg gcgtgatgtc gcggctgacg ctggtcggcg cgatctatat
    10861 caccgcggtc t
//
LOCUS       DATCZV010000401         2639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_510023, whole genome shotgun sequence.
ACCESSION   DATCZV010000401 DATCZV010000000
VERSION     DATCZV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510023"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VIW27_09090"
     CDS             complement(<1..898)
                     /locus_tag="VIW27_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5739868.1"
                     /translation="MKITFLGGAETVTGSKFLLETDAARVLVDCGLFQGYKWLRQRNW
                     QSPSIDVDTLDAVILTHAHLDHSGYIPVLYRHGFRGPVYCHAATRDLCGILLPDSGHI
                     QEDDARYYARHKLGKHENPLPLYDRATAEASLQLFHALDFDDRVSIGDLSFFLQPAGH
                     ILGAASVIVDARGKRIGFSGDVGRGNDVLMRSPRPLPPLDMLLLESTYGNRRHAQQEP
                     FAELAGLVNRTAQKGGVVLIPAFAVGRAQLLQHLLVSLMQSGEIARMPVYLDSPMAIS
                     VSDIFCRHRELHRLTEDECMLMC"
     gene            complement(895..1812)
                     /locus_tag="VIW27_09095"
     CDS             complement(895..1812)
                     /locus_tag="VIW27_09095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY5739869.1"
                     /translation="MTDAAQGPVLFVLQRHAIAGGLARHIAAQAGQFETRQFPDGETY
                     LQVATTVERRDCVVLADLSRPNDKYLPLIFLLETLRDLGAASVGLVAPYLCYMRQDNR
                     FTEGEAVTSRVFAADLCRHIDWLVTVDPHLHRYRSLDEIYTSCNRAVSSAPALSAWLG
                     KKSGLLLVGPDAESEQWVAGIAADSGRPFVVGSKQRRGDRDVLVTLPDLGEFRGHTAV
                     IVDDVIASGGTLLQCIDALRRGGIERISCLAVHGIFEDGADRRLLAAGLEQLITTNTV
                     PHPSNAVDVSGLIAPAVIDCIGRSEANKT"
     gene            complement(1809..>2639)
                     /locus_tag="VIW27_09100"
     CDS             complement(1809..>2639)
                     /locus_tag="VIW27_09100"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624925.1"
                     /note="catalyzes the formation of thymine and
                     2-deoxy-alpha-D-ribose 1-phosphate from thymidine; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY5739870.1"
                     /translation="ADMRRVVELTGACLAWGGAVNLSPADDLLIRIERALDLDGEGQL
                     IASVLSKKIAAGSTHAVIDIPVGASAKVRSRPEAERLASMFVQVSEACGIESRCVLTD
                     GSQPVGQGIGPTEEARDLLAVLQTTPEAPRDLRQRALTLAAQLFDMATAEGYEACLGK
                     ATEILDDGRAWRQFQRIVEAQGGFRQLPEARFSEVLSAPLGGEIRSIDNRRLARVAKL
                     AGAPSSRAAGLRLHVRIGSIIERGMPLFTLNANTEGERDYALEYYVGQENIFEVGDPT
                     "
BASE COUNT          463 a          847 c          854 g          475 t
ORIGIN      
        1 cgcacatcag catacactcg tcttccgtca ggcggtgcag ttcacgatgg cgacagaaga
       61 tgtcgctgac gctgatcgcc atcgggctgt ccaggtaaac cggcatccgc gcgatttcac
      121 cgctttgcat caggctgaca agcaggtgtt gcagtaactg cgcgcggccc accgcgaacg
      181 cgggaatcag caccactcca cccttttgcg cggtacggtt gaccagtccc gcgagctccg
      241 caaacggctc ctgttgcgcg tggcgccgat tgccataggt cgattccagc agcagcatat
      301 ccagaggcgg tagcggtctc ggggaacgca tcaggacatc attgccacgc cccacgtcac
      361 ccgaaaagcc gatgcgtttg ccccgggcat cgacgatgac gctggcggcg ccgagaatat
      421 gacctgcggg ctgcaggaaa aagctgagat ccccgatcga caccctgtcg tcgaaatcca
      481 gcgcatggaa cagctgcagg ctggcctcgg cggtcgcacg gtcgtagagc ggcagcgggt
      541 tttcgtgctt gccgagcttg tgccgagcat agtagcgggc gtcgtcttcc tggatgtgtc
      601 cactgtccgg aagcagaatc ccgcacagat cgcgggtcgc cgcgtggcag tataccgggc
      661 cgcgaaaacc gtggcggtaa agtaccggaa tatagccgga atgatcgaga tgcgcgtgtg
      721 tcaggatcac cgcgtcgagc gtatcgacat cgattgacgg cgattgccag ttgcgctgac
      781 gcagccactt gtagccctgg aacaggccgc agtcgacgag cacccgggcg gcgtcggttt
      841 ccagcaggaa tttggagcct gttacggtct ctgctccgcc gagaaacgtg attttcatgt
      901 cttgttcgct tcgctacggc cgatgcagtc aatcaccgcg ggtgcgatca agccgctgac
      961 atctaccgcg ttcgacgggt gcgggacggt gttggtggtg atcagctgct ctagcccggc
     1021 ggccagcagc cggcggtctg cgccgtcttc gaaaatgccg tgcaccgcga ggcagctgat
     1081 gcgctcgata ccgccgcgcc gcaatgcgtc gatgcattgc agcagcgtcc cgccgcttgc
     1141 aatcacgtcg tcgacgatga ccgcggtgtg tccccggaac tcgccgagat cgggcagcgt
     1201 gacgagcacg tcacggtcgc cgcggcgctg cttgctgccg acgacgaagg gccgcccgct
     1261 gtcggcggcg atgccggcca cccattgctc gctttccgcg tcgggaccga ccagcaacag
     1321 gccggatttt ttcccgagcc aggccgaaag tgccggggcg ctgctgaccg cgcggttgca
     1381 ggatgtatag atttcgtcga gcgagcggta acggtgaaga tgcggatcca ccgttaccag
     1441 ccagtcgatg tggcggcaca gatcggctgc gaaaaccctc gaggtaactg cttcgccctc
     1501 ggtgaaacgg ttatcctggc gcatgtaaca caggtaaggc gcaaccagcc cgaccgaggc
     1561 ggcgcccagg tcgcgcaggg tctccagcag gaagatcaac ggcaggtatt tgtcgtttgg
     1621 gcgggacagg tcggccagca ccacgcagtc gcgccgttcg acggtcgtcg cgacctgcag
     1681 gtaagtctca ccgtccggga actgccgcgt ttcgaactgt cccgcttgcg cggcgatatg
     1741 gcgagccagg ccgcccgcga tggcgtgccg ctgcaggacg aacaacacgg ggccttgcgc
     1801 tgcatccgtc atgtcgggtc cccgacttcg aaaatgtttt cctggccgac gtaatattcg
     1861 agcgcgtaat cgcgttcgcc ttcggtattg gcgtttagcg tgaacagcgg catgccgcgc
     1921 tcgataatgg agccgattcg cacgtgcagg cgcagtccgg ccgcgcggct cgagggcgcc
     1981 ccggcgagct ttgccacgcg cgccaggcgg cgattgtcga tactgcgaat ttcgccgccc
     2041 agcggtgcgc tcaatacctc gctgaaacgg gcttcgggca gttgcctgaa gcccccctgg
     2101 gcctcgacga tgcgttgaaa ttgccgccag gcgcgaccgt cgtcgagaat ctcggtggcc
     2161 ttccctagac aggcttcgta gccttcggcg gtcgccatgt cgaataattg ggcggccagc
     2221 gtcagcgcgc gctggcgcaa atcccgcggc gcttcgggag tggtctgcag caccgccagc
     2281 agatcgcggg cttcctcggt cggtccgata ccctgaccga cgggctgact gccgtcggtc
     2341 agcacgcagc gcgattcgat gccgcaggcc tcgctcacct gtacgaacat cgaggcgagg
     2401 cgctcggcct cgggccggct gcgtaccttg gcgcttgcgc cgactgggat atcgatcacg
     2461 gcatgcgtcg accccgcggc gatcttcttc gataacacgg aagcgatcaa ctggccctcg
     2521 ccgtccaggt cgagggcgcg ttcgatacgg atcagcaaat cgtcggccgg gctcaggttg
     2581 accgcgcccc cccaggccag gcaggccccg gttagctcga ccacccgccg catgtcggc
//
LOCUS       DATCZV010000402         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_721830, whole genome shotgun sequence.
ACCESSION   DATCZV010000402 DATCZV010000000
VERSION     DATCZV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721830"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="xthA"
                     /locus_tag="VIW27_09105"
     CDS             complement(<1..782)
                     /gene="xthA"
                     /locus_tag="VIW27_09105"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY5739871.1"
                     /translation="MKIVSFNVNSVRQRLHQLQRLIDRHAPEIIGLQETKVQDSEFPL
                     QEINDLGYQAIYHGQKTHYGVALLSRQRCAQPCYGFPNDGEEAQKRFIGGHFEFGGRP
                     LLVFNGYFPQGESRDHPVKFPAKQRFYRDLTEFLGGYDMMRSNLVVMGDYNIAPQDGD
                     IGIGEDNARRWLRTGKTSFLPEEREWFARLTGLGLHDGFRLCHADEDNVFSWFDYRSR
                     GFEREPKRGLRIDHLLVSELLRPAVVDAGVDYDIRAMKKPSD"
     gene            complement(763..2112)
                     /locus_tag="VIW27_09110"
     CDS             complement(763..2112)
                     /locus_tag="VIW27_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY5739872.1"
                     /translation="MLLRDRVIHYIWDDSAYGELAPLSEWRRFLQIITLVGRDLMSGM
                     ITLHAMGLVYTTLLSLVPLLAVSISVLKGFGVHGQLEPALAGVLSHLGDRSAEVSARI
                     VGFVDNMKFGVLGALGLGMLIFTVISLIQKIEAAFNHAWRLQGSRGLRQRFSDYLSVI
                     MVGPLLVFAAVGITASLGSSQVVAMLNELPFMNTLTHLLGKLLPFMLVIGAFTFVYLL
                     VPNTRVRLQSALYGAVVAGFLWEISGMLFTSFVGGSTQYTAIYSGFAIMLVFMLWLYL
                     SWIILLVGASIAFYHQNPEYLVWQQREIRLSTRQREQLALQAMVDIARTHDRQSDLQT
                     SLQNLARIQRIPAALLLRVLDALQAAGLVQQSADDPPRYLPGCSLERLRIADIVSIAR
                     QASTRSQRDSVLRSDASVTELLDGLDASLESYLGDLTLAELIQRTENSDTADEDRLV"
BASE COUNT          442 a          646 c          655 g          390 t
ORIGIN      
        1 tgatccgacg gttttttcat cgcgcgaata tcatagtcta caccggcatc gaccaccgcg
       61 ggcctcagga gttccgagac cagcaggtga tcgatgcgca gaccgcgttt gggctcgcgc
      121 tcgaaacccc ggctgcgata atcaaaccag ctgaagacgt tatcctcgtc ggcatggcag
      181 agtcgaaatc catcgtgcag cccgagcccc gtcaggcgcg cgaaccattc gcgttcctcg
      241 ggcaggaaac tggtcttgcc ggttcgcagc cagcgcctgg cattgtcttc gccgataccg
      301 atgtcgccgt cctgcggcgc gatattatag tctcccatca ccaccaggtt cgagcgcatc
      361 atgtcgtagc cgccgaggaa ctcggtcagg tcacggtaaa aacgctgctt ggcaggaaac
      421 ttgaccgggt gatcgcggct ctcgccctgc ggaaaataac cgttgaaaac cagcagcggc
      481 cggccgccga attcgaaatg tccgccgatg aaccgttttt gcgcctcttc gccatcattg
      541 ggaaatccat agcagggttg cgcgcagcgc tggcgtgaca gcaaggcgac gccgtaatgg
      601 gttttctgcc cgtgatagat cgcctggtag cccaggtcgt tgatctcctg cagcgggaac
      661 tcgctgtcct gcaccttggt ttcctgcaaa ccgatgattt caggggcgtg cctgtcgatc
      721 aggcgctgca gctggtgcaa gcgctggcgc acgctgttga cgttaaacga gacgatcttc
      781 atcggcggta tcactgtttt cggttcgctg tatgagttcg gcgagggtca ggtcgccgag
      841 ataggattcg aggctggcat cgaggccatc gagcaattcg gtcacgctgg cgtcactgcg
      901 caatacgctg tcgcgctggc tgcgggtgct cgcctgccgg gcgatactga cgatatcggc
      961 aatgcgcagg cgctcgagcg agcaccccgg caggtagcgc ggcgggtcgt cggccgactg
     1021 ctgcaccagt cccgctgcct gcagcgcgtc gagcacgcgc agcagcagcg ccgccggtat
     1081 ccgctgaatg cgcgcgaggt tttggaggct ggtctgtaaa tcgctctgtc ggtcatgggt
     1141 gcgcgcgatg tcgaccatcg cctgcaacgc caactgctcg cgctggcgag tactcaggcg
     1201 aatctcgcgc tgttgccata ccaggtactc ggggttctgg tggtaaaaag cgatactcgc
     1261 gccgaccagc agtatgatcc agctcaggta aagccagagc atgaacacca gcatgatcgc
     1321 gaagccggag taaatcgctg tgtactgggt cgagccgccg acgaaggacg tgaacaacat
     1381 acccgagatt tcccataaaa atccggcgac gaccgctccg taaagcgccg actgcagccg
     1441 cacgcgggta ttcggaacca gcaggtagac gaaggtaaaa gcaccgatca ccagcatgaa
     1501 aggcagcaac ttgccgagca ggtgcgtcag cgtgttcatg aatggtaatt cgttgagcat
     1561 cgcgaccacc tggctgctgc cgagagacgc ggtgatcccg accgcggcga acaccagcag
     1621 cgggccgacc atgatcacgc tcaggtaatc gctgaagcgc tgccttagac cgcggctgcc
     1681 ctgcagccgc caggcatgat tgaacgcggc ttcgattttc tggatcagcg agatcaccgt
     1741 gaaaatcagc atgccgagac cgagggcacc gagcacaccg aatttcatgt tgtcgacgaa
     1801 gccgacgatg cgcgcactga cctcggcact gcggtcgccg agatgggaca gcacgccggc
     1861 cagcgccggt tcgagctggc cgtgcacacc gaaacccttg agcacggaga tgctgaccgc
     1921 gagcagcggt accagcgaca gcagcgtggt atagaccaac cccatcgcgt gcagcgtgat
     1981 catgccgctc atcaggtcgc ggccgaccag cgtgatgatc tgcaggaagc gacgccactc
     2041 ggaaagcggc gcgagttcgc cgtaagcgct atcgtcccaa atgtagtgaa tgactcgatc
     2101 gcgcagcagc acggcttaac cggtacaggc ctg
//
LOCUS       DATCZV010000403         3137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_510112, whole genome shotgun sequence.
ACCESSION   DATCZV010000403 DATCZV010000000
VERSION     DATCZV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510112"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VIW27_09115"
     CDS             complement(<1..606)
                     /locus_tag="VIW27_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY5739873.1"
                     /translation="METTLTVAEDAPRLSSSPLRFALYFYLQSRYTLAAIFLFEAAQA
                     ACTILLPYAIKEIIDAVTLAQRTDADVFVTAKDALILFALLNLGILLFSRASGAMLVI
                     TGPRLRREIRRSLFSYIQHHSHRYFISHFAGSLANRIAEVSMSCMHALWTVTFDFYPL
                     IIKSAVALVVLFGASGELALTLSLWLGIYVYVSFLLARRCRV"
     gene            complement(914..2455)
                     /locus_tag="VIW27_09120"
     CDS             complement(914..2455)
                     /locus_tag="VIW27_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739874.1"
                     /translation="MRDVVRLLLVFPLFFIGSLAVYADQAGVQRGGYYEADCGSVRHH
                     GYRRQRQHAARLGAGCKQNRAVELTVVLEGTGSGRVSSNRPGIECQTDCNQTYNKNAR
                     IRLSAQPYSGSVFSGWSGSCSGTGSCRVKLQADSAVSAHFMRIETPPLSLVTPYVDEH
                     EMRDINDYFNAQYDSLPWGRIHDGLDIDPRQNLGAYQSVCAGRVRKIYVFDNQVTLII
                     DCDSTYSVDYNFETQAPATGQRQLRYILVTEGQRVAQGQELGYLYSAANPERAHVHFT
                     LYENAVPVCPAPFFTPAARDSILNLLAVVHTDGVICRSGNLLPPPLATPYVDEADMAR
                     ITAGYSAAYSFSPWGYPHDGLDIYPQGDLQPLQAACSGLIDKLELQRDGKHGHWQVEV
                     AVVCEDYVDDPEDGGYFIPLTTRYVLRTMSRDPLAGQAQLDNTMVGLGDPVVQGTVIA
                     YLKQASPQSHLHFELLQFGQSEFEAFGVTAIPRCPRAQFSPAARDSVSSLLQSTWPGA
                     EMCYQ"
     gene            2826..>3137
                     /locus_tag="VIW27_09125"
     CDS             2826..>3137
                     /locus_tag="VIW27_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739875.1"
                     /translation="MKLFAGFSVDTIVMRHKILILNLLLALVLLPVTQAMSPDAAPAE
                     HDHASMVMDCGHVDPGHCIDFASCVSGSHASCDTNLKAAQVLPKSADLPGALDLAEHF
                     LS"
BASE COUNT          630 a          963 c          944 g          600 t
ORIGIN      
        1 gacccggcag cgcctggcga gcaggaagga gacatagacg tagataccaa gccacaggct
       61 cagggtcagt gcgagttcgc cgctggcgcc aaacagcacg accagcgcca ccgctgactt
      121 gatgatcagc ggataaaaat cgaaagtaac cgtccacagc gcgtgcatgc aactcatcga
      181 cacttcggcg atgcggttcg ccagcgagcc ggcgaaatgg gaaatgaagt agcggtgcga
      241 atgatgctgg atgtaactga acagcgagcg ccgaatctcg cggcgcaggc gcggcccggt
      301 gatcaccagc atcgcgccgc tggcgcggct gaacagcagg atgcccagat tgagcaacgc
      361 gaacaggatc agcgcgtcct tggcggtgac gaagacgtcc gcatcggttc gctgcgccag
      421 ggtaaccgcg tcgatgatct ccttgatcgc gtagggcagc aggatggtac aggccgcctg
      481 cgccgcttcg aacaggaaaa tcgccgccag cgtgtagcgc gactgcaggt aaaaatagag
      541 ggcaaagcgc aacggcgagc tcgacaggcg cggtgcatcc tcggcgacgg tcagggtcgt
      601 ttccatgctc gcagtgtaat cgcaagccgg cgcgaaaagt agacgctgcc gcgagcgatc
      661 atcaccaatt ccggcaaccg cggcttggta actttcaacc tggcgttgcc gcctgccggg
      721 cagctcgatt accgactaca gcggagtcga ttcgatcccc gggagcgctg atatgggcac
      781 cgtatcgacg ctttacctgc agcgagtttg cggtggcagg caatcgactc gcggatcggt
      841 ccggcatcga tttacgctac ccttcggcac gggccgcgat ccgcgcatcg tcaggcttac
      901 gcgacggggg cggtcactgg taacacatct ccgcgccggg ccaggtcgat tgcagcaggc
      961 tcgagacgga atcccgtgcc gccggcgaaa actgcgctcg cgggcagcgc gggatagcgg
     1021 tgacgccgaa tgcctcgaat tccgactggc cgaactgcag cagttcgaaa tgcaggtggc
     1081 tttgcgggct ggcctgcttc aggtaggcga tgaccgtgcc ctgcaccacg ggatcgccga
     1141 gcccgaccat cgtattatcg agttgcgcct gtccggccag cggatctctt gacatggttc
     1201 gcagcacata cctggtcgtc agcggaataa aatagccgcc atcctcgggg tcgtcgacgt
     1261 aatcctcgca gaccaccgcg acttcgacct gccagtgtcc atgcttgcca tcccgttgca
     1321 attccagttt atcgatgagc cccgaacagg ccgcctgcag gggttgcagg tcgccctgcg
     1381 ggtaaatatc caggccgtcg tggggatagc cccagggcga aaaactgtag gccgcgctgt
     1441 accccgcggt aatccgggcc atgtccgctt catcgacata gggcgtggcc agcggcggcg
     1501 gcagaaggtt accgctgcga cagatgactc cgtcggtatg caccaccgcg agcaggttca
     1561 gaatcgagtc gcgcgccgcc ggggtaaaaa acggcgccgg gcagactggc accgcgttct
     1621 cgtagagcgt gaaatgcacg tgagcccgct ccgggttcgc cgccgagtac agataaccca
     1681 gctcctgccc ctgcgcgacc cgctgccctt ccgtcaccag gatataccgg agttgtctct
     1741 gcccggtagc gggcgcctgg gtttcgaagt tgtaatcgac ggaataggtt gagtcgcaat
     1801 cgataatcag cgtcacctgg ttatcgaata cgtagatttt cctgactctg cctgcacaga
     1861 cggattgata ggcccccagg ttttgcctgg gatcgatatc gaggccgtcg tgaatcctgc
     1921 cccagggcag gctgtcatat tgcgcgttga aataatcgtt gatgtcgcgc atctcgtgct
     1981 catcgacata gggcgtcacc aggctgagcg gcggcgtttc gatacgcatg aagtgcgcgc
     2041 tcacggcgct atcggcctgc agcttgactc tgcagctgcc ggtgccgctg caagacccgc
     2101 tccagccgct aaacacgcta ccggaatacg gctgcgcact gagccgaatg cgcgcgtttt
     2161 tgttataggt ctggttgcaa tcggtctggc attcgatgcc cggccgattc gagcttaccc
     2221 ggccgctgcc ggtgccttcg agaaccaccg tcaattcgac cgcgcggttt tgtttacagc
     2281 cggcgccgag ccgcgctgcg tgctgccgct ggcggcggta cccatggtgc cgaacgctgc
     2341 cgcaatcggc ctcgtaatat ccgcctcgct gcacgccggc ctggtcggca tagaccgcta
     2401 gcgagccgat aaaaaacagg ggaaaaacca acagcagtcg aacaacatcg cgcatgacaa
     2461 cctcccgtat cattcgagac ccgcgccttc aatatcgagg cacttgcccg caatcaatga
     2521 tatgacttgc cggagaacga tggttgatat cggtcagtaa tctctcgccg tccgcggctt
     2581 cgcctgtaat tcgcatgccg tgccatacgc agccgaccgc gagtcgtttg cttgcaagcg
     2641 gccagccgca cccgttgcat tgactccctg ccatgggaaa ctcttgagtg ataccggccc
     2701 gcccgccatc tcgacatcgg ccggattcgc ggccctgaag atcaacgcgc ggtatgcatg
     2761 aataaacaat atttttaccc ggcggcggcg actatggtct atgatttgac tgaatttcga
     2821 acagtttgaa actattcgcg ggtttctcgg tcgacactat cgtaatgcgg cataaaatcc
     2881 tgatattgaa cctgcttttg gcactggtgt tactgccggt gacacaggcc atgagccctg
     2941 acgcggcacc cgcggaacat gaccatgcgt ccatggtcat ggactgcggc catgtcgacc
     3001 ccgggcactg catcgacttc gcttcctgcg tgtcgggcag ccatgcgagc tgcgatacca
     3061 atctcaaggc ggcgcaggtt ttgccaaagt ctgccgatct gccgggcgcg ctggatctag
     3121 ccgagcactt cctttcc
//
LOCUS       DATCZV010000404         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_669192, whole genome shotgun sequence.
ACCESSION   DATCZV010000404 DATCZV010000000
VERSION     DATCZV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669192"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VIW27_09130"
     CDS             <1..200
                     /locus_tag="VIW27_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739876.1"
                     /translation="RPEVFCLQKAQSATDQQIFIPGNVLIHAPAKNATCQIDRQFRYQ
                     VIAKVFAIVIRRHKGWHRFGD"
     gene            240..1163
                     /locus_tag="VIW27_09135"
     CDS             240..1163
                     /locus_tag="VIW27_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739877.1"
                     /translation="MPASARHDNLKLAIGAILFGCLGLSLGDALIKQSSASFVLWQIF
                     FVRSAILLPFLIYIVRLQDCRTPLRPLRLGWTLLRSLVLVSSWIFYFAALPHIGLANA
                     AAALYTLPIFIALFAALFLGESIRGRGWLAVVLGFAGTLLILQPEAGDFEAYALLPIG
                     AAICYAAAMILTRSHCRGESPTLLSLWLNLAFIAVGALGLGVLSLWSPAAETVALNPF
                     LLGPWTPLELEGWRTMAILAAAILVGSVGAAVAYQQGPASIVAIFDFSYLPFAILWGH
                     LLFDDLPSPTVAGGMLLIVVAGTLAVRQRRG"
     gene            complement(1276..1599)
                     /locus_tag="VIW27_09140"
     CDS             complement(1276..1599)
                     /locus_tag="VIW27_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEY5739878.1"
                     /translation="MSNDIKDNLKSQSTWIRGLYMLLYIIFSRIAELVLGVVIVFQFL
                     LKLFTGETNERLLKLGQGLSTYIYQVFQYLSFNTEYQPYPFGAWPKGEPKPAKLSDQS
                     AATDD"
     gene            1834..>2411
                     /locus_tag="VIW27_09145"
     CDS             1834..>2411
                     /locus_tag="VIW27_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY5739879.1"
                     /translation="MRNLRRHHRHAGPPAAAAILLTMLCALSSVAADTPDPRQLIQAA
                     MEHWRGVTSYSEMTMTVHRPDWERSFSMQAWTEGDKLSLVRVTAPARDAGNGTLVKDR
                     QMWTYSPKINRILKLPSSMMNQSWMGSDFSNKDISKSTDIIDQYDHRLLSAEQQGDHL
                     RYTIESIPHEDAAVVWGREIAVIRDDYVLLRQ"
BASE COUNT          424 a          764 c          747 g          476 t
ORIGIN      
        1 gacgaccgga agtcttttgc ctacaaaagg cgcaaagtgc aaccgaccaa caaatcttca
       61 tccctggaaa tgttttaatc cacgcacccg cgaagaacgc gacttgtcag attgaccggc
      121 agttccgcta tcaggtcatc gccaaggttt ttgcgatcgt gattcggcgg cataaaggct
      181 ggcaccggtt cggggattga tacagactac cagcggtccc gataccacag ccgcgacaca
      241 tgccagcaag cgcccgccac gacaacctga agctcgccat cggcgcgatc ctgttcggct
      301 gcctggggct gtcgctgggc gacgcgctga tcaagcagtc gagcgccagc ttcgtgctct
      361 ggcagatttt tttcgtgcgc tcggcgatcc tgctgccgtt cctgatctac atcgtgcgcc
      421 tgcaggattg ccgcaccccg ctgcggccgc tgcgcctcgg ctggacgctg ttgcgcagcc
      481 tggtgctggt atcgagctgg attttctact tcgccgcgtt gccgcacatc gggctggcga
      541 acgcggccgc cgccctgtac acgctgccga tcttcatcgc gctgttcgcg gcgctgtttc
      601 tcggtgaatc gatacgcggt cgtggctggc tggcggtggt gctgggcttc gccggcacgc
      661 tgctgatcct gcagcccgag gccggcgatt tcgaggccta tgcactgctg ccgatcggtg
      721 cggcgatctg ctacgccgcg gcgatgatcc tgacccgcag ccactgccgc ggcgaatcgc
      781 cgacgctgct gtcgctgtgg ctcaacctcg ccttcatcgc ggtcggcgca ctggggctcg
      841 gagtattgag cttgtggagc cccgcggccg aaaccgtcgc actcaatccg ttcctgctcg
      901 gaccgtggac tccgctggag ctggaaggct ggcgaacgat ggcgatactc gcggccgcga
      961 tcctggtcgg cagcgtcggc gcggcagtcg cctaccagca gggtccggca tcgatcgtcg
     1021 cgatcttcga cttttcctat cttccgttcg cgatcctgtg gggtcacctg ctgttcgacg
     1081 atctgccgtc accaacggtc gccggcggca tgctgctgat cgtcgtcgcc ggcaccctgg
     1141 ccgttcgcca gcgccggggt tgatgtaacg agcggataca gcgggccgct actatcgcgc
     1201 agtggccggc attggctgac agggcctgac caggctgagg aacccgcgtg cagttgcggg
     1261 cccctggcca ggccgtcaat cgtcggtggc ggcgctctgg tcgctgagct tcgccggctt
     1321 cggctcgcct ttcggccagg cgccgaacgg gtacggctgg tactcggtgt tgaagctcag
     1381 gtactgaaaa acctggtaaa tgtaggtgct caatccctgc ccgagcttga gcaggcgctc
     1441 gttggtctcc ccggtgaaca gcttgagcag gaactgaaac acgatgacca cgcccaacac
     1501 cagttcggcg atccgcgaaa agatgatgta cagcagcatg tagaggccgc gaatccaggt
     1561 cgactggctc ttcaggttgt ccttgatgtc attactcatt gtcgtcttgt cctcgactgg
     1621 tgtgctaacg aatatcttcg cgcggaccgc cagatccggc aagcaccacc cggccccggg
     1681 ttgatctggc tcaaggtccg cgcggcgcga atccgaatag tatggaaccg agcctggcga
     1741 tggcacgcga accatcgccg cggtcttgtt acactgacgg ctttcttcga gactgccagc
     1801 gaatcgtttc gcgccaagta taccgacaag atgatgagaa atcttcgcag acaccaccgc
     1861 cacgccggtc cgccggcagc cgccgcaatc ctgctgacca tgctttgcgc actgtcttcc
     1921 gtcgccgccg atacgccgga tccgcgccaa ctgattcagg cggcgatgga gcactggcga
     1981 ggcgtgacct cgtactcgga gatgacgatg accgtgcatc gccccgactg ggagcgcagt
     2041 ttctcgatgc aggcctggac cgagggcgac aagctgtcgc tggtacgcgt caccgcgccg
     2101 gcgcgcgacg ccggtaacgg caccctggtc aaggacaggc agatgtggac ttactcaccg
     2161 aagatcaatc gcattctgaa gctgccgtcg tcgatgatga accagagctg gatgggcagc
     2221 gatttttcca acaaggacat cagcaagtcg accgacatca tcgaccagta cgaccatcgg
     2281 ctgctgtccg cggagcagca aggcgatcac ctgcgataca cgatcgagtc gattccgcac
     2341 gaggatgcgg cggtggtctg gggccgggaa atcgccgtga ttcgcgacga ttacgtgctg
     2401 ctgcgccagg a
//
LOCUS       DATCZV010000405         5397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_722062, whole genome shotgun sequence.
ACCESSION   DATCZV010000405 DATCZV010000000
VERSION     DATCZV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722062"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2432)
                     /locus_tag="VIW27_09150"
     CDS             complement(<1..2432)
                     /locus_tag="VIW27_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5739880.1"
                     /translation="MKNIALLLLLACTLPVAAATDAELERLEALRLAGDYQAALEQGR
                     DVLEVEPDDIRLLHLLGELELETGKLNAAASRFQRVIELSGQDSLMARSKLAEVHLQR
                     GERQEAAEQWRQIREAYQTRAGLSTRDLHSVAEATRHLGKSDPQLFKQAVRIFDDVLR
                     RDPANLDAGIALGELLLEKYNNQEALQVFQEVLSRDDRHPRAILGLARSQHFDYSSEA
                     VASTLEALSFNPNLVPARVLLARLFIELEQFADARHEAELALQVNPRSLEALATLAVI
                     HQLEHDQAGFEKIEARVLALNPGYAGLYTLLAEMSAQNRLYREAADFARRAVELDPES
                     WQGYGLLGMNQLRLGQMQAGRANLERSFSGDPYNVWIKNTLQLADSFSDYFEIDRGSF
                     RVVLHGDENALLEEYIHDLAQQAHRFFSTRYNHRPQLPIRIELYPDHADFSVRSVGLA
                     GVGLLGVSFGPVVAMDSPAARERGTFNWGSTLWHELAHVFHLSMTDNRVPRWFSEGLA
                     VYEERIARSGWGGDVDPGFLQAYLEGRLLPLSQLNNGFVRPSYPEQIIHSYFQASLVF
                     EFIEERWGFDTIRAILSAFRDSKMPHAVLLDALQLDSETLDAAFDAFLEDKYSIALQA
                     LSPSDDAASGRLSPDTALPDKYFDQFKLGTQLLEQGNIELAELFLTRAQQLFPEYAGG
                     DSSYWLLAQLYLKQERPDAAHQQLESMVAINAEHYEAHLQLARLRVGRGDIAGAAATL
                     EAALFIYPYDLEVHRDLARHLASLERWDEAARERRAVLALNPVDRAEAHYQLAHTYDR
                     AGNRSSAREQIL"
     gene            complement(2433..3398)
                     /locus_tag="VIW27_09155"
     CDS             complement(2433..3398)
                     /locus_tag="VIW27_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY5739881.1"
                     /translation="MKTVLAACLLSLASQTALALQHHLLIVSGIGGTEEYRRQFNSQA
                     ERLRQAALDAGLARDNIVMLAAESDGANYRPADKQSLLRAIREIDSRAGIEDRVFVIL
                     IGHGNARGDSALFNLPGPDISADELGAALDLLGERQLVIVNTASSSGPFIRRLAADNR
                     IVMTATASGQEFHAPLFGGFFVSAFAEAGADRDKDERISMLEAFDFARREVQRSYDSD
                     KRLLTEHALLDDNGDGEGSLEPGEYETDGMLANRVYLQQPRSAATGASSDLIAMLQRK
                     QELEQSIRDLKRQRETMERDSYYDRLESLLIDLALLSRKIRAGDS"
     gene            complement(3395..>5397)
                     /locus_tag="VIW27_09160"
     CDS             complement(3395..>5397)
                     /locus_tag="VIW27_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5739882.1"
                     /translation="PGVIGVLLDNSISMRLDHGDDPRSAYIEQQFDTERGPLLRSLRE
                     NFDTRLFSFGASTRAIEDITDLDFSDGDSDLQQALQFVQAALQGEPLAGLVVVSDGAV
                     QSSAQLEATLLAFKAAAIRVFGIGVGETQYQRDIEISRIEIPRQVLKGSRINARLSIR
                     QQGYDGETLDLQVVDDGRILHRQRIRIAAGEQSFEIPLATDDAGARQLEFKLGALPGE
                     SITANNRRQATLSVDPRKTRILYFEGEPRFEFKFVRRAVADDQQIALAGLMRTADAKF
                     YRVGIESEDELRNGFPITRDELFSYDALILGSVELALLSREQQDMIVEFVSRRGGGLL
                     MLGGRHAFAEGGYHESVLRDLSPAVMAPRAQPGFSRDIRLQPTAAALVHPALLLAPSS
                     DKSIARWLTLPPLTIVNPIQQIKPGATLLLASEAGDDEAAYVGMAFQRYGRGKVVAFP
                     VQNSWLWQMHQDIELEDQTHELLWRQLLRWLAQGVPERLDLTLSSHGIHSGGSMVLRS
                     EILQPDYSADNTAQARAVVTTPTGLEQVIDLARDTSLQGVYTAELTVTEPGNYQVRVE
                     LDQQGAMISSAESQFRVTPEGNEYYRSEMNPALLGQIAALTGGEFLPAGKTGGLAQAL
                     GQQRRGARSLTRHELWDMPVIFLLLILFLCAEWGYRRWRNLI"
BASE COUNT          969 a         1729 c         1679 g         1020 t
ORIGIN      
        1 tatagtatct gctcgcgtgc cgaagatcga ttgccggcgc gatcataggt atgcgccagc
       61 tggtaatgcg cctccgcccg gtccaccggg ttcagcgcaa gcacggcgcg gcgttcgcgg
      121 gcggcctcgt cccagcgctc gagactggcc aggtggcgcg ccaggtcgcg atgcacttcg
      181 agatcgtagg gataaatgaa caacgccgct tccagtgttg ccgccgctcc cgcgatatca
      241 ccgcgcccga cgcgtaacct ggccagttgc agatgcgcct cgtagtgctc ggcgttgatc
      301 gcgaccatgc tctcgagctg ctggtgcgcc gcgtcgggcc gctcctgctt caggtaaagc
      361 tgcgccaaca gccagtagct gctgtcgccg ccggcatatt ccgggaatag ctgctgcgcc
      421 cgcgtgagaa acagttcggc cagctcgata ttgccttgct cgagcagttg ggttccgagc
      481 ttgaactggt cgaagtactt gtccggcagc gcggtgtcgg gagaaagccg tccagacgcg
      541 gcgtcgtcac tcgggctcag cgcctgcagc gcgattgagt acttgtcctc gagaaaagcg
      601 tcgaaggccg cgtccagcgt ttcgctgtcg agctgcagcg cgtcgagtaa cacggcatgg
      661 ggcatcttgc tgtcgcgaaa agccgataaa atcgctcgaa tggtgtcgaa accccaacgc
      721 tcctcgatga attcgaatac cagcgaagcc tggaaatagg aatggatgat ttgctccggg
      781 tagctcgggc ggacgaaacc gttgttgagt tgactcagtg gcagcaggcg cccctcgaga
      841 taagcctgca ggaaccccgg atcgacgtcg ccgccccagc ccgaccgcgc aatgcgctct
      901 tcgtaaaccg cgagaccctc ggaaaaccag cgcgggacgc ggttgtcggt catcgacagg
      961 tggaacacat gcgccagttc gtgccacagg gtcgaacccc agttaaaggt gccgcgttcc
     1021 cgcgcggccg gcgagtccat cgcgaccacc gggccgaagc tgaccccgag caggccaacg
     1081 ccggccagcc cgaccgagcg caccgagaaa tcggcatggt cgggatatag ttcgatgcga
     1141 atcggcagct gcggccggtg gttgtagcgg gtggaaaaaa accggtgagc ctgctgcgcc
     1201 aggtcgtgga tgtattcctc cagcagtgcg ttttcatcgc cgtgcagcac cacgcggaag
     1261 ctcccccggt cgatctcaaa atagtcgctg aaactgtccg ctagctgcag cgtattcttg
     1321 atccatacgt tataaggatc gccgctgaac gagcgctcca ggttagcacg gcctgcctgc
     1381 atctgtccga gccgcaactg gttcatcccg agcaggccgt aaccctgcca cgactcggga
     1441 tcgagctcta ccgcgcggcg cgcgaaatct gccgcttcgc ggtagagccg gttttgggcc
     1501 gacatttcgg ccagcagcgt gtagaggccg gcgtaacccg gattcaatgc cagcacccgg
     1561 gcctcgattt tttcgaaccc tgcctggtcg tgttcgagct gatggataac cgccagggta
     1621 gccagggctt cgagcgagcg cgggttcacc tgcagcgcga gttcggcctc gtgccgcgcg
     1681 tcggcgaatt gctccagctc gataaaaagg cgcgccagca gcacgcgcgc cggcaccagg
     1741 ttgggattga aagacagcgc ctcgagggtg ctggcgaccg cctccgagga gtaatcgaaa
     1801 tgctggctgc gcgccaggcc gagaatggca cgcggatggc ggtcgtcacg cgacaggact
     1861 tcctggaata cctgcagcgc ttcctggttg ttgtattttt ccagcagcag ttctccgagc
     1921 gcgatgccgg cgtccaggtt ggcgggatcg cggcgcagca cgtcgtcgaa aatacgaacc
     1981 gcttgtttga acaattgcgg gtcggacttg ccgagatgcc gggtcgcctc agcgaccgag
     2041 tgcaaatccc gggtgctgag gccggcccgg gtctgataag cctcgcggat ttgccgccac
     2101 tgctctgcgg cctcctgccg ttccccgcgc tgcaggtgaa cctcggcgag ctttgaacgc
     2161 gccatcaacg aatcctgccc ggataattcg attacgcgct gaaagcggga tgcggcggca
     2221 ttcagcttgc cggtctcgag ctccagttcg cccagcaagt gcaatagacg gatgtcgtca
     2281 ggctcgactt cgagaacgtc gcgtccctgc tcgagcgcgg cctggtaatc gccggccagg
     2341 cgcaacgctt cgagccgttc gagctcggcg tcggtcgccg ccgcgaccgg tagcgtgcag
     2401 gccagtaaca gtagtaacgc gatgtttttc atttaactgt ctcctgccct gattttccgg
     2461 ctgagcagcg cgagatcgat caacaggctt tcgagccgat cgtaatagct gtcgcgctcc
     2521 atggtctcac gctgccgttt caagtcacgg atcgactgct ccagttcctg tttgcgctgc
     2581 agcatcgcta tcaggtcgct cgaagcgccg gtcgcggccg agcggggttg ctgcaggtaa
     2641 acacggttcg ccagcatccc gtccgtttcg tattcccccg gttcgaggct gccctcgccg
     2701 tcgccgttgt cgtccagcag cgcatgttcg gtcaacaggc gcttgtcgct atcgtagctg
     2761 cgctggacct cgcggcgggc aaagtcgaag gcctccagca tcgatatgcg ttcgtccttg
     2821 tcgcggtccg cccccgcctc ggcgaaggcg gagacgaaga aaccaccgaa cagcggcgcg
     2881 tgaaactcct ggccgcttgc ggtggcggtc atgacgatgc ggttatccgc tgccagccgc
     2941 ctgataaacg gtccactgga agacgcggta ttgacgatca ccagttgccg ttccccgagc
     3001 aggtcgagcg cggcgccgag ttcgtcggcc gaaatatcgg gtcccggcag gttgaacaac
     3061 gcgctgtcgc cgcgtgcatt tccgtgacct atcaggatta cgaaaacgcg gtcctcgatg
     3121 ccggccctgg agtcgatctc gcgaatcgct cgcagcaggg attgcttgtc ggccggacga
     3181 taattcgcgc cgtcgctctc cgcggccagc atgacgatgt tgtccctggc gagcccggcg
     3241 tcgagcgcgg cctgcctcag cctctctgcc tggctgttga actgccggcg gtattcctcg
     3301 gtgccgccga taccgctgac gatcagcagg tgatgctgca gcgccagggc agtttgcgag
     3361 gcgagcgaca gcaggcaggc ggcgaggaca gttttcatat caggttacgc cagcgtcggt
     3421 agccccattc ggcgcacagg aacaagatca gcaacaggaa aatcaccggc atatcccaca
     3481 gttcgtgacg cgtcagcgag cgggcgcccc gtcgctgttg ccccagggct tgcgccagcc
     3541 cgccggtttt gccggctggc aggaactcgc caccggtcag ggccgcaatc tggcccagca
     3601 gcgccgggtt catttcggac cgatagtact cgttgccttc gggggtgacc cggaattgcg
     3661 attcggcgct gctgatcatc gcgccctgct ggtcgagctc gaccctgacc tggtaattcc
     3721 ccggctcggt caccgtcagt tcagccgtgt agacaccctg cagcgatgta tcccgggcca
     3781 ggtcgatcac ctgctcgagc ccggtgggcg tggtcaccac cgcccgcgcc tgcgccgtat
     3841 tatcggcgct atagtcgggc tgcaggattt cgctgcgcaa aaccatgctg ccgccgctgt
     3901 gaataccgtg gctcgaaagc gtcaagtcca gccgctcagg gacgccctgc gccagccagc
     3961 gcaacaattg ccgccacagc agctcgtgcg tctgatcttc gagctcgatg tcctggtgca
     4021 tttgccacaa ccaggaattc tgtaccggaa acgctaccac cttgccgcga ccatagcgct
     4081 gaaaagccat ccccacgtaa gcggcctcgt cgtcgcctgc ctcgctggca agcagcaggg
     4141 tggcgcccgg cttgatctgc tgaatcgggt tgacgatggt tagtgggggc agggtcaacc
     4201 agcgcgcaat cgatttatcg ctacttgggg cgagcagcag ggccgggtgc accagcgccg
     4261 ccgcggtcgg ctgcagcctg atgtcgcggc tgaacccggg ttgagcccgc ggcgccatca
     4321 ccgccggcga taaatcccgc agtaccgact cgtggtagcc gccttcggcg aaggcgtgcc
     4381 gtccgccgag catcaacaga ccaccgccgc gacggctgac gaactcgacg atcatatcct
     4441 gttgctcgcg gctcaacagc gcgagctcga cgcttcccag gatcagcgcg tcgtaggaaa
     4501 acaactcatc ccgggttatc gggaaaccgt tacgtaactc atcctcggac tcgatgccca
     4561 cgcggtaaaa cttggcatca gcggtgcgca tcaggccggc cagcgcaatc tgttggtcat
     4621 cggcaaccgc gcggcgtaca aacttgaact cgaaacgggg ctcaccctcg aagtacagaa
     4681 tcctagtttt acgcgggtcg accgacaggg tcgcctggcg gcggttattg gccgtgattg
     4741 attcgccggg cagggcgccg agtttgaatt ccagttgccg cgccccggcg tcgtcggtgg
     4801 caagcggtat ttcgaacgac tgctcgccgg cagcgatcct gattcgctgg cggtgcagaa
     4861 tgcggccatc gtccacgacc tgcagatcca gcgtctcgcc gtcataaccc tgctggcgaa
     4921 tactcagcct ggcgttgatc cggctgccct ttaacacctg tcgcggtatc tcgatccggc
     4981 tgatctcgat atcgcgctgg tattgcgtct cgccgacccc gatgccaaac acccgtatag
     5041 cggcggcctt aaacgccagt aacgtggcct cgagttgcgc gctcgactgc accgcgccgt
     5101 cgctcacgac gaccaggccg gcgagcggtt ctccctgcag cgccgcctgc acaaactgca
     5161 gggcctgctg cagatcgctg tcgccgtcgg aaaaatcgag gtcggtaata tcttctatcg
     5221 cccgggtgct ggcgccaaag ctgaacaacc gggtgtcgaa gttctcacgc agagagcgca
     5281 gcagcggccc gcgctcggtg tcgaactgct gctcgatata ggcgctgcgt ggatcgtccc
     5341 cgtggtccag gcgcatgctg atcgaattat ccagcagcac gccgatcact ccgggtt
//
LOCUS       DATCZV010000406         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_34558, whole genome shotgun sequence.
ACCESSION   DATCZV010000406 DATCZV010000000
VERSION     DATCZV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34558"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VIW27_09165"
     CDS             complement(<1..465)
                     /locus_tag="VIW27_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739883.1"
                     /translation="MKKNTHHGLTHFATGILMAVALIVLGANGAAADSDPGLREQIAQ
                     KIALTRNYLESSTAKKIAESENPQARSMLEKSRELLEQAVEALDDGKLLAAQDKVNLS
                     LQSFTAAGTANMKKAVDSKQVERELGTVRAEIDAYLQSFTAALAEKGPAMAGL"
     gene            complement(599..1312)
                     /locus_tag="VIW27_09170"
     CDS             complement(599..1312)
                     /locus_tag="VIW27_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEY5739884.1"
                     /translation="MKSAWRDSPLEVGGLTPLTTVDYPGELAAVVFLQGCPWRCGYCH
                     NPQLVERRTDAPLAWPRVIEFLERRRGLLDAVVFSGGEPTLHAGLGEALRQVKSLGFK
                     TALHTAGVYPERLRRILPLLDWVGMDIKSPRACYASITGVRGSAERAWESIELLLESG
                     VECEFRTTLHPDLLDAAELTALADELGERGIGSLVLQKCVTSRCRDSALREVRRALPD
                     AHFLDRLGERFEHFSLRNF"
     gene            complement(1290..1454)
                     /gene="nrdD"
                     /locus_tag="VIW27_09175"
     CDS             complement(1290..1454)
                     /gene="nrdD"
                     /locus_tag="VIW27_09175"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEY5739885.1"
                     /translation="MSCTSPIKLKAEERTRCEIWTRVMGYHRPLGSFNPGKQSEYRER
                     KLFDEKRMAR"
     gene            complement(1432..>2194)
                     /gene="nrdD"
                     /locus_tag="VIW27_09180"
     CDS             complement(1432..>2194)
                     /gene="nrdD"
                     /locus_tag="VIW27_09180"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEY5739886.1"
                     /translation="ALMQQLDRLLDLARNSLEIKRKEIQRHIDAGLFPYTKRYLGTLR
                     NHFSTIGVNGINEMVRNFSADRDDITSEQGKALAIRFLDHVRARMLEFQEETGHMYNL
                     EATPAEGTTYRFAREDRKRYPDILQAGSDEQPYYTNSSQLPVGYTEDPFEALEMQDEF
                     QRKYTGGTVLHLYMNERISSIDACKRLVRRSLETFHLPYLTVTPTFSICPQHGYLDGE
                     HEFCPRCDEELLARKQAARLQTQTGRTDHVMHITH"
BASE COUNT          337 a          683 c          732 g          442 t
ORIGIN      
        1 caggccggcc atcgccgggc ccttttcggc cagcgccgcg gtaaacgatt gcaggtaggc
       61 gtcgatctcg gcgcgcaccg tgcccagctc gcgttccacc tgcttgctgt cgacggcttt
      121 tttcatgttc gcggtgccgg ccgcggtaaa gctctgtaac gacaggttga ccttgtcctg
      181 cgcggcgagc agcttgccgt cgtcgagcgc ttcgaccgcc tgctcgagta attcgcggga
      241 cttttcgagc atgctgcggg cctgcggatt ttcgctctcg gcaatctttt tcgcggtgct
      301 cgattcgagg taattacgcg tcagcgcgat cttctgcgcg atctgctcac gcaggccggg
      361 atcgctgtcg gcagccgcgc cgttcgcccc cagcacgatc aacgccaccg ccatcaggat
      421 gccggtagcg aaatgggtca ggccgtggtg ggtatttttc ttcaagccga taatcgagta
      481 tctcgtcggc gtctaggata ccattttggt tccggttgct gctacggcga gtggcgatta
      541 ccggcgctgc ccgccacccg ggaacccgcg cgacaggctc ggcgccggcg ccgccgggtc
      601 aaaaattgcg caggctgaaa tgctcgaagc gctcgccgag gcgatccagg aaatgcgcgt
      661 ccggcagcgc gcgcctgact tcgcgcaggg ccgaatcgcg gcagcggctg gtgacacatt
      721 tctgcagcac cagcgagccg atgccgcgct cgccgagttc gtcggccagc gcggtcagct
      781 ctgccgcgtc cagcaggtcc gggtgcaggg tggtgcggaa ttcgcactcg acaccgctct
      841 ccagcagcag ctcgatgctt tcccaggcgc gctctgcgga gccgcgtacc ccggtaatcg
      901 aggcgtagca ggcgcgcggc gacttgatgt ccatgcccac ccagtcgagc agtggcagga
      961 tccgtcgcag gcgctcgggg tagacaccgg cggtgtgcag ggcggttttg aagccgaggg
     1021 acttcacctg gcgcagggct tcgccgaggc cggcatgcag ggtcggctcg ccgccgctga
     1081 agaccaccgc gtcgagcaag ccgcggcgcc gctcgaggaa ctcgatcacc cgcggccagg
     1141 cgagcggtgc gtcggtgcgc cgctccacca gttgcgggtt gtggcagtag ccgcagcgcc
     1201 acgggcagcc ctgcaggaac accaccgccg ccagttcgcc cgggtaatcg acggtggtca
     1261 gcggcgtcag gccgccgacc tcgagcgggc tatcgcgcca tgcgcttttc atcgaacagc
     1321 ttgcgctcgc ggtattcgga ctgcttgccc gggttgaacg agccgagcgg tcgatggtaa
     1381 cccatcaccc gggtccagat ttcacagcgg gttctttctt cggctttcag tttaatgggt
     1441 gatgtgcatg acatggtcgg tcctcccggt ttgggtttgc aggcgggctg cctgcttgcg
     1501 cgccagcagt tcttcgtcgc agcgcgggca gaattcgtgc tcgccgtcga gatagccgtg
     1561 ctgcgggcaa atcgagaagg tcggcgtcac ggtcaggtac ggcagatgaa atgtttccag
     1621 cgaacgccgc accagtcgct tgcaggcatc gatactggag atgcgctcgt tcatgtacag
     1681 gtgcagcacc gtgcccccgg tatacttgcg ctgaaattcg tcctgcatct ccagcgcctc
     1741 gaatggatct tcggtgtagc cgaccggcaa ttgcgacgaa ttcgtgtaat agggctgctc
     1801 gtcgctgcct gcctgcagga tatcggggta gcgcttgcgg tcctcgcgcg cgaagcggta
     1861 ggtcgtgcct tccgcgggcg tcgcctcgag gttgtacata tggccggttt cttcctggaa
     1921 ctcgagcatg cgcgcgcgca cgtggtcgag gaagcggatc gccagcgcct tgccctgctc
     1981 gctggtgatg tcgtcgcgat cggcgctgaa attgcgcacc atttcgttga tgccgttgac
     2041 gccgatggtc gagaaatgat tgcgcaatgt gcccaggtag cgcttggtgt acgggaacag
     2101 gccggcgtcg atgtgccgct ggatttcctt gcgcttgatt tccagactgt tgcgcgccag
     2161 gtcgagcagg cggtcgagtt gctgcatcag ggcg
//
LOCUS       DATCZV010000407         3723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_722201, whole genome shotgun sequence.
ACCESSION   DATCZV010000407 DATCZV010000000
VERSION     DATCZV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722201"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..2225)
                     /locus_tag="VIW27_09185"
     CDS             complement(36..2225)
                     /locus_tag="VIW27_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5739887.1"
                     /translation="MLLAAFVKRYDRRRLRAMLGILFAVLVLPTGFIVWQAYDQLKWE
                     SWYQYRSQAEALAMRIDAELGERVSLSEARSFTDYAFLNATANASVLNRSPLSALPVP
                     EDLPGVIGYFQVDPEGAFSTPLLPASGEWLESGLTPEEYAARTRLETELRSILAGNQL
                     LRDGMASGGRLQAPAVAPQPAREERASAPGPGAIDQPAAVTVTVTDAADDVEPQARLA
                     QARLAEEAEMEAPAEAYAQRMFDQLNAPLYTESAAPQSAPAAALAVGAADDNAGNVSK
                     LRDLQLDDALEKKNEELARQADEPPSGKKSAATEQVRARRVEQSVLPAAPAEVADADR
                     ELDRLDNAITTFESEIDPFQFSLLDSGHLVLFRNVWRNGSRYIQGLLIDQPAFLEAVI
                     DSAFRSTTLFDMSDLVIGYHDDVIRLIRGGNYASLQSSAGEFDGALLYRGSLSAPFDR
                     LELVFSINRLPPGPGAKVLGWSALVIAAVFVGGFISLYRLGLGQIRLARQQQDFVSAV
                     SHELKTPLTSIRMYGEMLKEGWADEEKQKQYYEFIHDESERLTRLISNVLQLSRITRN
                     EPQFDARPVSVGKLLDQVRSKISSQVERAGFDLEFAVDDDAVDRRVEVDDDCFAQIVI
                     NLVDNAIKFAKNAPTKKIEISCRVDAGDKIVFRVRDFGPGIDKSQLKKIFRLFYRTES
                     ELTRETAGTGIGLAIVHQLVTAMGGSVDVVNCDPGAEFQVAFPAAGS"
     gene            complement(2229..2930)
                     /locus_tag="VIW27_09190"
     CDS             complement(2229..2930)
                     /locus_tag="VIW27_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5739888.1"
                     /translation="MNRKIRILVVEDEQAIREGLVDVLVFHHYDVDSAATGPEGLEKA
                     LTGKYDLILLDIMLPGMDGYEICDRVRREDREQPIIMLTAKTSDEEIVQGLKLGADDY
                     VPKPFSIQQLVLRIEAVLRRSRVGVASARTLSLAGIEIDTENLSGQRDGEEIPFTKRE
                     MEVLGYLARHSDRPVSREELLVKVWGYAKNLDIETRTVDIHIAKIRRKIETDPKAPRN
                     LVTVRGAGYRLVTAS"
     gene            3100..>3723
                     /locus_tag="VIW27_09195"
     CDS             3100..>3723
                     /locus_tag="VIW27_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5739889.1"
                     /translation="MSSKKSVFALVLCTCLLFAGCSPQPAEQNVAADPEIERLAARAA
                     GVTIIRDDFGVPHIYGKTDADAVFGLLFAQAEDDFPRIERNYIWATGRLAEVEGEEAL
                     YSDLRARLYMTIDEAKAAFDAAPQWLKDLCNAYADGLNWYLHTHPEVQPRLLTRFEPW
                     FPMFFSEGSIGGDIESISLRGIEAFYGGENPADALQAATPRFFEPKGS"
BASE COUNT          648 a         1184 c         1164 g          727 t
ORIGIN      
        1 gggcccggtg tcgctggctt gagctgctga ccgggtcatg atccggcggc gggaaacgcc
       61 acctggaatt cggcgccggg atcgcaattg accacatcca cgctgccgcc catcgcggtc
      121 acgagctggt gcacgatcgc aagcccgatg cccgtgcctg cggtctcgcg ggtcaactct
      181 gattcggtcc ggtaaaacag ccggaatatc ttcttcaact ggctcttgtc gatgcccggt
      241 ccgaagtctc gaacgcggaa cacgatcttg tccccggcat cgacccggca gctgatctcg
      301 atctttttcg tcggcgcatt ttttgcaaac ttgatggcgt tatcgacgag attgatgacg
      361 atctgggcaa aacagtcgtc gtcgacctcg acacgccggt caacggcgtc gtcgtcgacc
      421 gcaaactcga ggtcgaaacc cgcgcgctct acctggctgc tgattttcga gcgcacctgg
      481 tccagcaatt tgccgacgct caccggccgg gcgtcgaact gcggctcgtt gcgcgtgatc
      541 ctcgacagct gcaacacgtt cgagatcaag cgggtcagtc gttccgattc gtcgtggatg
      601 aactcgtaat attgcttttg cttctcttcg tcggcccagc cttccttcag catctccccg
      661 tacatgcgga tcgaggtcag cggcgtcttg agctcgtggc tcacggcgga gacgaaatcc
      721 tgctgctggc gggcgagccg tatctggcca agccccaggc gataaagcga aatgaagccg
      781 ccaacgaata cggcggcgat gaccagcgct gaccagccca gtaccttcgc accgggcccg
      841 ggcggcagcc ggttgatcga gaaaacgagt tccaggcggt cgaagggcgc tgacaggctg
      901 ccgcggtaca gcagggcgcc gtcgaattcg cctgcgctgg attgcagcga ggcatagttg
      961 ccgccacgga tcagcctgat aacgtcatcg tgatagccga tgacgagatc ggacatgtcg
     1021 aacagggtcg tgctgcgaaa cgccgaatcg atgacggctt ccagaaacgc tggctggtcg
     1081 atcaacaggc cctggatgta gcgactaccg ttacgccaga catttcggaa cagcacgagg
     1141 tgaccgctgt ccagcaggct gaactggaac gggtcgatct cgctctcgaa ggtcgtgatc
     1201 gcattgtcga gccggtcgag ttcccggtct gcatcagcca cctcggcagg cgccgcaggc
     1261 agcacggatt gctccacgcg ccgcgcgcgc acctgttcgg tcgctgcgct tttcttgcca
     1321 gaaggcggct cgtcggcctg ccgggccaat tcttcgttct tcttctcgag cgcgtcatcg
     1381 agctgcagat ctcgcagttt gctgacgttc ccggcgttgt catcggccgc acccacagcg
     1441 agcgctgcag ccggcgccga ctgcggtgcc gcgctctccg tgtacagcgg cgcattcagc
     1501 tgatcgaaca tgcgctgcgc ataggcttcg gccggcgctt ccatctccgc ttcttccgcg
     1561 aggcgagcct gtgcaagtct cgcctgcggt tcaacatcgt ctgctgcatc ggtaacggtg
     1621 acggtgacgg cggcgggctg gtctatcgca ccgggcccag gtgcgctggc gcgttcctcc
     1681 ctggccggtt gaggagcgac ggccggcgcc tgcaggcgtc ccccggaggc catgccatcg
     1741 cgcaacagct gatttccggc caggatactg cggagttccg tttcgagccg ggttctcgct
     1801 gcgtactcct cgggcgtaag gccggattcg agccattcgc cgctggccgg cagcagcggc
     1861 gtcgagaacg ccccctcggg atccacctgg aaatagccga tcacgccggg caggtcctcg
     1921 gggaccggca gtgccgagag gggcgagcga ttgaggacgc tggcattcgc ggtggcgttg
     1981 agaaacgcgt agtcggtgaa gctgcgggcc tcggacagcg agacgcgctc gcccagctcg
     2041 gcgtcgatcc gcatggccag cgcttcggcc tggctgcgat actgatacca ggactcccat
     2101 ttgagctggt cataggcctg ccagacgatg aacccggtcg gcagaacgag cacggcaaac
     2161 aggatgccga gcattgcccg caggcggcgc cggtcgtatc gcttgacgaa agcggccagc
     2221 agcatggctc agcttgccgt gaccagccgg tagccggcgc cacgcacggt gaccaggttg
     2281 cggggcgcct tgggatccgt ctcgatcttg cgccggatct tggcaatgtg gatgtcgact
     2341 gttcttgtct cgatgtcgag gtttttcgca taaccccata ctttaacgag taattcttct
     2401 ctggaaaccg gtcgatccga atgccgtgcc aggtaaccga gaacctccat ttcccgcttg
     2461 gtgaaaggga tctcctcgcc gtcgcgctgg ccggaaagat tttccgtgtc gatctcgatg
     2521 ccggccagcg acagcgtccg cgccgacgcc accccgaccc gggagcggcg cagcaccgcc
     2581 tcgatgcgca gcacgagttg ctggatagaa aacggtttcg ggacgtagtc gtccgcaccg
     2641 agttttagcc cttgcacgat ctcctcgtcg gaagtctttg cggtcagcat gatgatcggc
     2701 tgctcgcgat cctcgcggcg cacccggtcg cagatctcgt agccgtccat gccgggcagc
     2761 atgatgtcga gcaggatcag gtcatatttg ccggtgagcg ctttttcgag cccttccggt
     2821 cccgtcgccg cgctgtccac gtcatagtgg tgaaaaacca gcacgtcgac caggccttcg
     2881 cgaatggcct gttcgtcttc cacgaccagt atgcggatct tccggttcat gaacccgagg
     2941 ctatcaggcg gcgtcgcccc tgtgcgccgt cgaatgtaac gtttttgtaa aaacaggcat
     3001 ttatgcgaac tcgtcgcccc tttccggcag ccccgcattc ctgtaaaaaa gcggcacgcc
     3061 ggccatacga taaacggcgc tctgtccacc gggggttcca tgtcatcgaa gaaatctgtc
     3121 tttgcgctcg tcctttgcac ctgcctcctg tttgccggct gttcgccgca gcctgcggag
     3181 caaaacgttg ccgccgatcc cgagatcgag cgcctggcgg cgcgggcggc gggcgtcacc
     3241 atcatccgcg acgacttcgg cgtgccgcat atctacggca agacggacgc ggacgcggtt
     3301 ttcggcctgt tgtttgcgca ggcggaggac gatttcccga ggatcgagcg caattacatc
     3361 tgggcgaccg gccggctcgc ggaggtcgag ggcgaggagg cgctgtacag cgacctgcgg
     3421 gcgcgcctct acatgacgat cgacgaggcc aaagcggcct ttgatgcggc gccgcagtgg
     3481 ctcaaggacc tttgcaacgc atacgccgac gggctcaact ggtacctgca tacccacccg
     3541 gaagtccagc cgaggctgct gacccgcttc gagccgtggt tcccgatgtt cttcagcgag
     3601 ggttcgatcg gcggcgacat cgagagcatc tccctgcgcg gcatcgaggc cttttacggc
     3661 ggcgaaaacc cggcggatgc cctgcaggcg gccaccccga gattcttcga accgaaggga
     3721 tcg
//
LOCUS       DATCZV010000408         7194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_616620, whole genome shotgun sequence.
ACCESSION   DATCZV010000408 DATCZV010000000
VERSION     DATCZV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616620"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIW27_09200"
     CDS             <1..237
                     /locus_tag="VIW27_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018144123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5739890.1"
                     /translation="HPLRLKILCILGDREICVQGIVENVGTTQSNISQHLAILRDKGI
                     LSSRKDANKVYYRVGDERTLQLIRMMQEVFCSTG"
     gene            266..694
                     /locus_tag="VIW27_09205"
     CDS             266..694
                     /locus_tag="VIW27_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5739891.1"
                     /translation="MMSQIPEFAANHPFLVLAFAVLLALTFFNEMKIATQKFASLTPA
                     GAVKLMNDGDVVVVDVREPSETVGGKIAKAIQIPYSSISKRIGELDKHKGKTLLVYCK
                     TGARSGAACRELSKSGFEKVYSLNGGIMGWQEAHLPISKK"
     gene            702..1169
                     /gene="secB"
                     /locus_tag="VIW27_09210"
     CDS             702..1169
                     /gene="secB"
                     /locus_tag="VIW27_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY5739892.1"
                     /translation="MSQEQETKKQFSIQKLYVKDVSFESPGAPGSFKFKNWEPKIELN
                     LSNEQQQIDGDLHEVVLRITATVSHDGNTAFLAEVHQGGLFVVAGFDEEEKRYLLGSQ
                     CMSTLFPYAREAVSDLSIRGGFPPLLLSPVNFDAVYQQHLQRQQESEAAPQEH"
     gene            1169..2161
                     /locus_tag="VIW27_09215"
     CDS             1169..2161
                     /locus_tag="VIW27_09215"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5739893.1"
                     /translation="MTDTVAVLGAGSWGTALAIQLARNDLQVRLWGHKREHIERLRAL
                     CENTDYLPGFRLADNIEPQASLPTAVEDAGYLLVAIPSKGFRDLLEQLKPLIDESPIV
                     FWASKGFEIETGKLLHEVVEDELPRQRYGVVSGPTFAAEVAQNLPAAITCAGNEPLAT
                     EAFAELLRAEHFRAYTTDDVIGVELGGALKNVLAIAVGIADGLGYGANTRAALITRGL
                     AEIMRLGISLGARQETLMGLAGLGDIILTCTDDQSRNRRFGLALGKGKSVRQAEIEVG
                     QTVEGLRAAKAIHDKAQAMGLELPIIEQAYLVLYEGKPPKEAVSDLESRPQGRE"
     gene            complement(2167..2625)
                     /locus_tag="VIW27_09220"
     CDS             complement(2167..2625)
                     /locus_tag="VIW27_09220"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY5739894.1"
                     /translation="MLHIVLFEPEIPPNTGNIMRLAVNTGCRLHLIEPLGFVLDDRQL
                     RRAGLDYREWAQIQTYRDFADFSARSGCTRLFAFTTRASNPYTDAEFGAGDALLFGPE
                     SRGLPDTILQSLSPERRLRIPMVPGSRSLNLSNSVAVAVYEAWRQLDFAD"
     gene            2821..2979
                     /locus_tag="VIW27_09225"
     CDS             2821..2979
                     /locus_tag="VIW27_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739895.1"
                     /translation="MRRIITTYISVVLLVSLGFFGVGTLASAQQSTVVKQASQAVPAA
                     AGELLIGN"
     gene            complement(2966..3277)
                     /locus_tag="VIW27_09230"
     CDS             complement(2966..3277)
                     /locus_tag="VIW27_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5739896.1"
                     /translation="MHKSQQGFAMVEAVLAAVLLGILTVTILGQFGDPLNGSAMHGNV
                     DAKTSSAAAINYTARVLDPVVFVALEAGNDCASGESHSAFPFDYGYPGFFNAGNEANC
                     R"
     gene            3644..5038
                     /locus_tag="VIW27_09235"
     CDS             3644..5038
                     /locus_tag="VIW27_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5739897.1"
                     /translation="MPLGSEKKPTLLLVDDDTIIADSLEYVLRDEYEIARASDRPSSF
                     ELIERMAVSPALALVDLGLPPNTHRPDEGLAIIRKLSQKHPSTRVLVLSGQDNKQHVF
                     KAKEDGAVDFIAKPCDVAEIRMRLKQQLVAGGSNDQGQGDIEGIIGSSREIELLRMQI
                     RQIADSPYPVLIEGESGSGKEVTARNLHRCSSRSPEPFLTLNCAAFNGELLESQLFGH
                     LKGSFTGATDTKKGFFEEAGDGTLLLDEVADLPLDLQAKLLRVLEDGEYYRLGETQPR
                     HASARVLAATNKDIFAAIRAGQFREDLYHRLSVLTVRVPSLRERNGDSQELLFYFMQQ
                     LTEQLSAFKLDDEALKLWNNYTFPGNVRELRNIIIRLSAKYPGQTIKPEVLKREMSAT
                     QRHNGAISGDDWLLKALNNAGFELDRLLKTVEKKAIDLALREHGGNVSRAADMLGINR
                     TTLYGRMERLEDKD"
     gene            5042..6760
                     /locus_tag="VIW27_09240"
     CDS             5042..6760
                     /locus_tag="VIW27_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5739898.1"
                     /translation="MYEDYFGLERPPFKITPDTSLFYEGGKRGDILDALVYAIQRGEG
                     IVKVVGEVGSGKTMLCRMLQLKLPDSVEIVYIANPSVSPEDILFVIAHELSLPVSREA
                     SKHKVMHSLQDYLLKRHMENRQVVLFVEEAQGMPLETLEEIRLLSNLETDQNKLLQII
                     LFGQPELDENLSKQSIRQLRERITHNFNLEPLSREEIYNYLNFRMREVGYRGPELINR
                     AVAKKVEQHSGGLLRRINIIADKILLSAYAEGTHNLSAKHVDAAVNDSAFDSVVTRRG
                     GAIWWFALLLLAALLFALYQTRGEWLSAAAAVVSPEQLQARDIDGATADAEFRQLTAR
                     QPAGGRSSDTAEHHGSGMPLPAEVVEIAFGVGSEVGRTSTQTLVDPAADILNSGEDFE
                     VIVETEQAVTVVAQIPSLRPPSAVDNETPGRSAPAADDGRQHVKPLAIAAVRPASDVD
                     GTPKDSAARVEDEYGRWLDAKLEQSREWLSGVDGSKVSIQVMVREKSAVRELVYYLLN
                     DWPLDLSRTYVYEVNLKNRSIYRILYSEFDTVAVGKRQIEKLPDSVKNNSPYLHSIHR
                     LQKGLL"
     gene            6788..>7194
                     /locus_tag="VIW27_09245"
     CDS             6788..>7194
                     /locus_tag="VIW27_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEY5739899.1"
                     /translation="MKRLLVLLALFSLMSCQALSEKDDRMAVSTTHLDSAVPEPAVEQ
                     SEIPPVVEQVPIISEPEPAEPLQTFTVVATDLPASELLFALARDARLNLDIDPRIEGR
                     VSINAIDQTLPQILKRISRQVSLRYYLDGPNLVV"
BASE COUNT         1557 a         2013 c         2187 g         1437 t
ORIGIN      
        1 catccgctgc ggctgaagat cctctgcatt ctcggcgacc gcgagatctg cgtgcagggg
       61 atcgtcgaaa atgtcggcac cacgcaaagc aatatttctc aacatctcgc gattttgcgc
      121 gataaaggta tactgtcgag ccgcaaggat gcgaacaagg tatattatcg cgtcggcgac
      181 gagcgtaccc tgcaattgat tcgcatgatg caggaagtat tctgttcgac cggctagtac
      241 actaaagcat tcaagttttc gatttatgat gtctcaaatc cccgagtttg cggcgaacca
      301 tccgttcctg gtccttgcct ttgcggtatt gctggcgctg accttcttca acgagatgaa
      361 aatcgccacg cagaaattcg ccagcctgac gcccgccggc gcggtcaaat tgatgaatga
      421 cggggatgtg gtcgtagtcg acgtgcgcga accgtcggaa accgtgggtg gcaagattgc
      481 caaggcgatc cagataccct acagctcgat ttcgaagcgc atcggcgaac tcgacaaaca
      541 caagggcaaa acgctgctcg tctattgcaa gacgggcgcg cgctcgggtg ccgcctgccg
      601 ggaattgagc aagagcggct tcgaaaaggt atacagcctg aatggcggta tcatgggctg
      661 gcaggaagca cacctgccga tcagcaagaa atagagcgaa tatgagccag gaacaggaaa
      721 ccaaaaagca gttttcgatc cagaaacttt acgtcaagga cgtctctttc gagtcgcctg
      781 gcgcgcccgg gtccttcaaa ttcaaaaact gggaaccgaa aatcgaactc aacctgagta
      841 atgagcagca gcaaatcgac ggcgatctcc acgaagtcgt actcagaatt accgcgaccg
      901 tcagccacga tggtaacacg gccttcctgg ccgaggtcca tcagggcggc ctgttcgtgg
      961 tcgctggctt cgatgaagag gaaaagagat acctgctcgg atcgcagtgc atgagtacgc
     1021 tgttcccgta cgcgcgcgag gccgtctccg acctgtcgat tcgcggcggg ttcccgccac
     1081 tgctgttgtc gccggttaat ttcgatgcgg tctaccagca acacctgcag cgacaacagg
     1141 aatccgaggc ggcgccacag gagcactgat gaccgacacc gttgcggtgc tgggcgcggg
     1201 ttcctgggga acggcactgg cgatccagct ggcccgcaac gatctgcagg taaggctctg
     1261 gggacacaag cgcgaacata tcgagcgctt gcgagcgctg tgcgaaaata ccgattacct
     1321 gcccgggttc aggctcgccg acaacatcga gccgcaggcg tccctgccaa ccgcggtcga
     1381 ggatgccggc tacctgctgg tcgccattcc gagcaaggga ttcagagatc tgctggagca
     1441 gctgaaaccg ctgatcgacg aatcgccgat cgtgttctgg gccagcaagg gcttcgagat
     1501 cgagaccggc aagctgctgc acgaggtggt cgaagacgag ctgccgcggc agcgctacgg
     1561 cgtggtctcc gggccgacct ttgccgccga ggtggcgcaa aatctgccgg ccgcgatcac
     1621 ctgtgccggt aacgaaccgc tcgccaccga ggcgtttgcc gaattactgc gtgccgagca
     1681 cttccgcgcc tataccaccg acgacgtgat cggcgtcgaa ctcggcggcg cgctgaagaa
     1741 cgtgctcgcg atcgcggtcg gcatcgccga cggcctcggc tacggtgcca atacgcgtgc
     1801 tgcgctgatc actcggggac tggcggaaat catgcgcctc ggcatcagcc tcggagctcg
     1861 ccaggaaacc ctgatgggtc ttgccgggct cggtgatatc attcttacct gtaccgacga
     1921 ccagagccgt aaccgccgct tcggactcgc gctcggcaag ggcaagagtg tgcggcaggc
     1981 ggagatcgaa gtcggccaga ccgtggaagg cctgcgcgcg gcgaaagcga tccacgacaa
     2041 ggctcaggcg atgggactcg agctgccgat catcgagcag gcatacctgg tgctgtatga
     2101 gggcaagccg ccgaaggagg cggtcagcga tctcgaaagc cggccccagg ggcgcgaata
     2161 gcgccgctag tcggcgaagt ccagctggcg ccaggcctcg taaacggcga ccgcgacgct
     2221 gttcgacagg ttgaggctgc gcgagcccgg caccatcggg attcttaagc ggcgctccgg
     2281 cgacagggat tgcagtatcg tgtcgggcag gccccggctc tctggcccga acagcagagc
     2341 gtcgccggcg ccgaactcgg cgtcggtgta tgggttgctg gcacgggtgg tgaacgcgaa
     2401 tagtcgcgta caaccgctgc gtgccgagaa atcggcgaag tcgcggtaag tctgaatctg
     2461 cgcccattcg cgataatcga ggccggcacg ccgcagctgg cggtcgtcga gaacgaatcc
     2521 cagtggttcg ataaggtgca ggcggcagcc ggtattgacc gccaggcgca tgatattgcc
     2581 ggtattgggt ggaatttcgg gctcaaacag gacgatatgc agcatcgagt aatttttttt
     2641 gccaagttcc ttgcaaaagc ctgtttttga attagtattt tcagcgaaca caaagagtta
     2701 taacgatatt tttaagaaat atctagactg gcttgcggtt tcgagtaacg cgagcctggc
     2761 cgccagcttg acggctcaat ggggtaagcc cgtcagggta aatcgataac aggaacacga
     2821 atgcgccgca ttattactac ttatatctcg gtcgtgctgt tggtttcgct aggattcttc
     2881 ggcgtcggca cgctggcttc ggcccagcag agcacggtcg tgaagcaggc ttctcaggct
     2941 gtgccggcgg ctgccggcga gctgcttatc ggcaattagc ctcgttcccg gcgttgaaaa
     3001 agccgggata gccgtaatcg aacggaaatg ccgagtgaga ttcgccactc gcgcaatcat
     3061 tgcctgcttc cagggcgacg aaaacaaccg ggtccagcac gcgtgcggtg tagttgatcg
     3121 ctgccgccga cgaggttttg gcatcgacat tgccatgcat cgcgctgccg ttcagcgggt
     3181 cgccgaattg cccgagtatg gtcaccgtga ggattcccag cagcacggct gccagtaccg
     3241 cttcgaccat tgcgaaaccc tgttgtgact tgtgcatgct gttgactcct tggcgctcgg
     3301 gatggcgagc ctgaatgtta cttgtgccgc cgcccgccgg cgggtgaaag cgtctcacgg
     3361 acgcctgctc ggtcaactaa tgagtgtagt aaatattccc cgtttttcag gaaattcggc
     3421 gttctgcgac agccggcagg gcgacaatcg gccggtaccg ttgacgaaaa gcttgatcgg
     3481 tttaacagcg tcgcgaatgc ttcggcgata aacttgaaaa ttaacgttca tatttggatt
     3541 ttctccatat atttcaagtg gtttcatttc gaaattaaag tatggtttga gttgttgaaa
     3601 actagacact atactcaatc gattaaactc cggggttttc gggatgccgc tgggcagtga
     3661 aaaaaaaccg acgctactgc tggtcgacga cgataccatt atcgccgata gcctggagta
     3721 cgtgctgcgc gacgaatatg aaatcgcccg tgccagcgat cggccttcga gcttcgagct
     3781 gatcgagcgc atggcggtct cgccggcgct ggcgctggtc gatctgggac tgccgccgaa
     3841 tacccaccgc cccgacgagg ggctggcgat cattcgcaag ctgtcgcaga agcacccttc
     3901 tactcgcgta ctggtgttgt ccggccagga caacaagcag cacgtattca aggccaagga
     3961 agacggtgcc gtcgatttca tagccaagcc ctgcgatgtt gccgagatca ggatgcgcct
     4021 caagcagcag ctggtcgccg gtggcagcaa cgatcaggga cagggtgata tcgagggcat
     4081 catcggttcg agccgcgaaa tcgaattgtt gcgcatgcaa atacggcaaa tcgccgattc
     4141 cccgtacccg gtgctgatcg agggtgagtc gggcagcggc aaggaagtca cggcacgcaa
     4201 tctgcatcgc tgcagctcgc gcagccccga gcctttcctg acattgaact gtgctgcttt
     4261 caatggcgaa ttgctggagt cacagctgtt cgggcacctc aagggttctt ttaccggggc
     4321 caccgatacc aagaagggat ttttcgagga agccggcgac ggcacgctgc tgctcgacga
     4381 ggtggccgat ttgccgctgg atctgcaggc caagctgctg cgcgtcctcg aagacggcga
     4441 gtactaccgt ctcggagaga ctcagccgcg ccatgcctcg gcacgcgtgc tggcggcgac
     4501 caacaaggat attttcgccg ccatcagggc ggggcagttt cgcgaggatc tctaccaccg
     4561 gctgagcgtg ttgacggtgc gcgtgccgtc cctcagggag cgcaacggtg attcccagga
     4621 actactgttt tacttcatgc agcagctgac cgagcagcta tccgccttca agctcgacga
     4681 cgaggcgtta aagctttgga ataattatac tttcccggga aatgtgagag aattacgcaa
     4741 tataataatc agactttcag cgaagtaccc cgggcagacc atcaagcctg aggtgttgaa
     4801 aagagaaatg tcagcaacac aacggcacaa tggcgcgatt agtggtgacg attggctgct
     4861 caaggctctg aataatgccg ggttcgaact cgaccggctg ctgaagacgg tcgagaaaaa
     4921 ggctatcgac ctggcgcttc gcgagcacgg cggcaacgtc agcagggctg ccgatatgct
     4981 cggcatcaat cgcactaccc tttacggacg catggagcgt ctcgaggaca aggattagtt
     5041 tatgtacgag gattatttcg gtctcgaacg accgccgttc aagataactc cggacaccag
     5101 cctgttctat gagggtggca agcgtggcga tatcctcgac gcgctggtgt acgcgattca
     5161 acggggcgaa ggtattgtca aggtagtcgg cgaagtcggc agcggcaaga ccatgctgtg
     5221 tcgtatgttg cagctcaaac tgccggattc ggtcgaaatc gtctatatcg ccaatccgag
     5281 cgtatcgcct gaagatattc tcttcgtcat cgcccacgaa ctctcgttgc cggtatcgcg
     5341 ggaggcgagc aagcacaagg ttatgcacag cctgcaggat tacctgctga agcggcatat
     5401 ggaaaacagg caggtggtgt tgttcgtcga ggaggcgcag ggaatgccgc tggagacgct
     5461 cgaggaaatt cgcctgctgt ccaacctcga aaccgaccag aacaagctgc tgcaaatcat
     5521 tctcttcggt cagccggagc tcgacgaaaa cctgtcgaag caatcgatac gccagttgcg
     5581 ggagcgcatc acgcacaact tcaacctcga gccgctgagt cgtgaagaaa tctacaacta
     5641 tctgaatttt cgcatgcgcg aggttggtta tcgggggccc gagttgatca accgcgcggt
     5701 ggcgaaaaag gtggagcaac attcgggcgg tctgttacgg cgaatcaata tcatcgccga
     5761 caagatcctg ctgtccgcct acgccgaggg cacccataat ctgagcgcca agcatgtcga
     5821 tgcggcggtc aacgacagcg ctttcgacag cgtcgtgacg cggcgtggcg gcgccatctg
     5881 gtggtttgcg ctgttattgc tggcggcgct gttgttcgcg ctctatcaaa cccgtggaga
     5941 atggctgtcg gctgcggcag cggttgtcag cccggagcag ttgcaggcgc gcgacattga
     6001 cggcgcgacc gccgatgccg agttccggca gttaactgcc cggcaaccgg cggggggcag
     6061 gtcgagcgat accgcggagc atcacggcag cggtatgccg ctgcctgccg aggttgtcga
     6121 aatcgccttc ggtgtcggtt ccgaggtcgg gcggacctcg acgcagacgc tcgtggaccc
     6181 cgccgccgac atcttgaact ccggtgagga cttcgaggtc atagtcgaaa ccgagcaagc
     6241 ggtgaccgtc gtcgcgcaaa tcccgtcgct gcggccgccg agtgctgttg acaacgaaac
     6301 acctggacgg tcggctccgg ccgcagacga cgggcggcag catgtcaagc ccttggcgat
     6361 cgccgcagtg cggccggcca gcgatgtcga cggcacaccg aaagattcgg cggcgcgggt
     6421 cgaagacgaa tacggccggt ggctcgatgc caagctcgaa cagagccgtg aatggctatc
     6481 cggggtggat ggcagcaagg tttcgatcca ggtcatggtg cgtgaaaagt ccgccgtgcg
     6541 tgaattggtc tattatttgc ttaatgactg gccgctggat ctgtcacgga cttatgtcta
     6601 cgaggtgaat ctgaaaaatc gcagtattta ccggattctt tacagcgagt tcgatacggt
     6661 ggctgtcggc aaacggcaaa tcgaaaaact accggattcg gtcaagaata attcacccta
     6721 tctgcattcg attcaccgct tgcagaaagg gttgctgtag cagcctgcat tcaactggaa
     6781 tgacacgatg aaaagactct tagtgttact cgccctgttc agcctgatgt cctgccaggc
     6841 attgtctgaa aaggacgatc gcatggcagt ctcgacgaca cacctcgatt ccgcggttcc
     6901 cgagccggcg gtcgagcaaa gcgagattcc gccggtggtc gagcaggtgc cgattatctc
     6961 cgagcccgaa cccgccgaac ccctgcagac gtttaccgtg gtggcgaccg atttgccggc
     7021 cagcgaactg ttgttcgcgc tggcgcgcga tgcgcgcctc aacctcgaca tcgacccgcg
     7081 tatcgaaggc cgtgtcagta tcaatgccat cgaccagacc ctgccgcaga tactcaagcg
     7141 aatctcgcgc caggtttcgc tgcgctatta cctcgacggc ccgaacctgg tggt
//
LOCUS       DATCZV010000409         9089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_351980, whole genome shotgun sequence.
ACCESSION   DATCZV010000409 DATCZV010000000
VERSION     DATCZV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351980"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VIW27_09250"
     CDS             <1..1311
                     /locus_tag="VIW27_09250"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY5739900.1"
                     /translation="RSTGIALALAAVFSLLSLWLLTIVAPALQSSSINLIGASFLYVL
                     CLFFVPALLLGIPTPLLTTLALRLDQRTGHIVGRMHALAALGSIVGTFLTGFVLIQYL
                     GSRAVVSLSSLLLLLLALPFLRGLSGRAVAALLVVTAASIALTRGSDGFASPCDRESS
                     YFCLRVVDASAQAPFGNARGLVLDHLLHGINHAEDPGMLISPYVHLMDELVRQHFAAE
                     QLDELRYFFAGGGSYTQPRAIRHQTPGAAVTVAELDPLVTEIAARDLYVDPAEFEIHH
                     GDARSLLFHRQPGEFDVVVTDAFHDIAIPYHLVTLEYARLVRSRLKPGGLFTTNVVDA
                     FPDPKMVKSLVKTLQQEFAQVDIWLDRIPSQPERLTYVISASDRRIDSDLLRASHGFE
                     RNWLRINQPLLGSGTPLERLPVFSDDFVPVERMISDLLLTREGL"
     gene            1439..1810
                     /locus_tag="VIW27_09255"
     CDS             1439..1810
                     /locus_tag="VIW27_09255"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEY5739901.1"
                     /translation="MIRFINCVRRRQDISLEEFRRHWNSAEIEVLYERLNAIVQPLRF
                     SRNLTLQVGANELIRDERGSGEPFDGTVEFWWQNAGDLLQKYDSNEARRVRESMLDLQ
                     ERFIDMGGCRAFFTEYPDAQP"
     gene            complement(1819..2046)
                     /locus_tag="VIW27_09260"
     CDS             complement(1819..2046)
                     /locus_tag="VIW27_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739902.1"
                     /translation="MTKRHRVDEPAARAGDTLERLRARVRGGGYLLLDSLDDDELVAM
                     QSLLLSGEAEIVNSACKPFLVAKLDRVILPP"
     gene            complement(2046..2723)
                     /locus_tag="VIW27_09265"
     CDS             complement(2046..2723)
                     /locus_tag="VIW27_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5739903.1"
                     /translation="MRIQSLNVGKPRLVEHKGERVWTGIYKDASDAPLMARKLNIDGD
                     QQADPGVHGGIDKAVYAFPCEHYGYYQQQLGQQDYAPGQFGENLTTEGLLESRVHIGD
                     RYRTGGALLEVSQPRSPCYKFAIRMGVAEALAVCINSARTGFYLRVLREGEIRAGDAI
                     ELEYANLEAPTVEAVHRLYYLDRRNLEGLRQAVLCDRLAADFRDEFRARLEKIESASQ
                     SSISEEH"
     gene            complement(2833..4488)
                     /gene="betA"
                     /locus_tag="VIW27_09270"
     CDS             complement(2833..4488)
                     /gene="betA"
                     /locus_tag="VIW27_09270"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY5739904.1"
                     /translation="MDQEYDYIIVGAGSAGCVLANRLSEDHAVKVALLEFGGSDSSIF
                     IQMPTALSIPMNMKKYNWFYESEPEPYLDNRRMHCPRGKVLGGSSSINGMVYVRGHAR
                     DFDEWQSQGARDWDYRHCLPYFKKADDWAFGSDEYRARGGPLAVNNGNNMQNPLYRAF
                     IEAGEQAGYMKTADYNGRQQEGFGPMHMTVNNGVRWSTANAYLKPALQRPNLTVITGA
                     LTQRILLEQRRAVGVEFLRAGSTQILKARREVLLSAGPIASPQLLQLSGIGPGAVLQR
                     AGLEVVHDLPGVGENLQDHLEFYFQFRCRQPITLNAELSPWRKFLIGLRWVLTKKGLG
                     ATNHFESCAFIRSRAGIEWPDIQYHFLPGAMRYDGRAAFDGHGFQVHVGHNKPNSRGH
                     VRARTPRVQDKPEILFNYLQHADDIEAFRACVRLTREIIGQPAMDAYRGPEIQPGEAV
                     QSDAEIDAFVRQAVESAYHPSCACRMAVVDSETRVRGIDGLRVVDSSIFPTIPNGNLN
                     SPTIMVAERAADIIKGKGMLEPSAAPVGVGRDWQTLQRSTAAD"
     gene            4595..4960
                     /locus_tag="VIW27_09275"
     CDS             4595..4960
                     /locus_tag="VIW27_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5739905.1"
                     /translation="MDITSATRALGALSQESRLKAFRLLVQSGADGIAAGKIAETLAI
                     PHNTMSTHLATLVNAGLAVSRRESRSIIYRVDFNGTRELLSFLVEDCCQGRPEACLPA
                     LESVLPGCCAPNQPSGVNS"
     gene            4957..5412
                     /locus_tag="VIW27_09280"
     CDS             4957..5412
                     /locus_tag="VIW27_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY5739906.1"
                     /translation="MKRLHVNVSVTDLEASVGFYSALFDAAPTLLKSDYAKWMLEDPR
                     VNFAITSRGQRKGIDHLGIQVEDDAELDEVYARLKAADAPTIEQGETTCCYANSTKSW
                     IFDPDSIAWETFLTRGESAVYGSDEMLAREQASACCSRPQAKDTAGNCC"
     gene            5471..5977
                     /locus_tag="VIW27_09285"
     CDS             5471..5977
                     /locus_tag="VIW27_09285"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY5739907.1"
                     /translation="MPEQKANVLFLCTGNSARSVIAESLINRLGHGRFSGYSAGSHPA
                     GDVHPLARSVLKKNDYPTDGLRSKSLEEFSRPGSPVMDFVFTVCDRAAAEVCPLWPGQ
                     PMSAHWGTADPAAVEGSEIDRRAAFRSTFDELHSRISIFVNLPFDFLDRLELKQRLDE
                     IGRSRPAD"
     gene            6020..7036
                     /gene="arsB"
                     /locus_tag="VIW27_09290"
     CDS             6020..7036
                     /gene="arsB"
                     /locus_tag="VIW27_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEY5739908.1"
                     /translation="MGTFERYLSVWVALCIAAGVILGVVMPGFFESIAAIEYASVNLV
                     VAVFIWIMIYPMMVNVDFASLKDVGKKPRGLCITLVVNWLIKPFTMAALGILFFEYIF
                     AGLVEPQTAKEYIAGMILLGVAPCTAMVFVWSQLVRGDANYTLVQVSINDVIMVFAFA
                     PLAALLLGVTDIVVPWETLLLSVVLYVLIPLAAGYATRKMLDGSRNHARIEQFTTGIK
                     PFSVMGLLATVVLLFGFQAETIIDNPAVIALIAIPLLIQSYAIFVVAYGWAYLWRVPF
                     TTAAPAALIGTSNFFELAVAVAISLFGLDSGAALATVVGVLVEVPVMLSLVAFANRTR
                     HHFA"
     gene            complement(7097..8440)
                     /locus_tag="VIW27_09295"
     CDS             complement(7097..8440)
                     /locus_tag="VIW27_09295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5739909.1"
                     /translation="MAIPSHVKYLVIGAGIHGLSTAYHLAQKLKATGNGDGRDILVVD
                     KGGIAAGASGIACGVVRNNYFQPAMRELMAHSVGVWETDPEGYSYHPVGYMQISPESM
                     HEDVASIARQQKEIGYESVFIEGEKDSMGYMKNLFHDWQAKGITSVLHEKRGGYANNT
                     KAMYALAGKAEAEGVRILTGCTVTGFSSDSAGGAIRAVETDRGEIGCDQVVIGAGPWA
                     KTVWDMLELPKTISIKGADGTVHENVPMWIYWSLQEGTLGVDPELQKTNDGKFPPVIH
                     VDSNEPLYSDFDGSLITDKMWGIYYKPDFHFGGIQGGAAPYTVDRDPDDVAVDPYGVD
                     SPDFVVGEDFAHMWTSALAHCQKRFEGQFEFYKNEPSGGIGCFTPDSFPVFDVFRENC
                     YLIADSNHGYKMLGVGKLVAEELCGEKSALLEPFRFSRYAEGRLHPVSNSPFPWS"
     gene            complement(8601..>9089)
                     /locus_tag="VIW27_09300"
     CDS             complement(8601..>9089)
                     /locus_tag="VIW27_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5739910.1"
                     /translation="RDIEQRTITLIAPSKTFNLAGLGCAFAIVADPGLRRGMKTNPFI
                     LPHVSITGLVAAEAAYRHGDEWNRQQCAYLAANRDYLVDEINRIPGLGLGPVEATYLA
                     WIDVSALQLEQPPRFFEEAGVGMSPGFEFGDANFMRLNFGCPRSRVEQAVERIRAAVE
                     AL"
BASE COUNT         1628 a         2944 c         2785 g         1732 t
ORIGIN      
        1 cgcagtaccg gcatagcgct ggcgctggca gcggtgttca gcctgctgag cctgtggctg
       61 ctgacgatcg tcgcgccggc actgcagtcg agttcgatca acctgatcgg cgcaagcttt
      121 ctgtacgtgc tgtgcctgtt cttcgtgccg gcattattgc tcggcatacc gacgccgctg
      181 ctgaccacgc tggcgttgcg gctcgaccag cgtaccgggc acatcgtcgg ccgcatgcat
      241 gcgctggcgg cactcggcag catcgtcggc accttcctga ccggattcgt gctgatccag
      301 tacctcggtt cgcgcgccgt cgtcagcctg tcgtcactgc tgctgctatt gctcgcattg
      361 ccttttctgc gcggcctatc gggccgtgcg gtcgcggcat tgctggtggt gaccgcggcc
      421 agtatcgcgc tgacccgcgg cagcgacggc tttgccagtc cctgcgaccg tgaaagcagt
      481 tacttctgcc tgcgcgtagt cgacgcctcg gcacaggcac cgttcggcaa cgcgcgcggg
      541 ctggtactcg atcacctgct gcacgggatc aatcacgccg aggatcccgg catgctgatc
      601 tctccctacg tgcacctgat ggacgaactc gtacggcagc atttcgcggc cgagcaactc
      661 gacgaactgc gctacttctt cgccggcggc ggctcttaca cgcagccgcg cgcgattcgg
      721 caccaaaccc cgggcgccgc ggtgaccgtg gccgagctcg atccgctggt cacggagatc
      781 gccgcacgcg acctgtatgt cgatccggcc gagttcgaga tccaccacgg cgacgcccgc
      841 agcctgttgt ttcaccgcca gccgggtgaa ttcgacgtcg tggtcaccga tgcgtttcac
      901 gacatcgcca tcccctatca cctggtcacg ctggagtacg cccggctggt gcgttcgcgg
      961 ctgaagccgg gcggcttgtt cacgaccaac gtcgtcgacg cctttcccga tccgaaaatg
     1021 gtcaagagcc tggtgaaaac gctgcagcag gaattcgcgc aggtcgacat ctggctggac
     1081 cgcattccgt cgcagcccga gcgcctgacc tatgtgatct cggccagcga ccggcgcatc
     1141 gacagcgacc tgctgcgggc cagccacgga ttcgagcgca actggctgcg aatcaaccag
     1201 ccattgctgg gctccggcac cccactcgag cgcctgccgg tgttcagcga cgacttcgtg
     1261 ccggtggaac gcatgatctc cgacctgctg ctgacccgcg agggcctgta ggcactgtaa
     1321 tcggtggacc gcggcccgcg actccgcggc gcaaaaacca ctgtgggcat tcaaaaaaat
     1381 gctatcttaa aatcgattag cgcatgctgc agtcaattag ccgacaggaa ccccgaccat
     1441 gattcgattt atcaactgcg tacgccgccg gcaggatatt tcgctcgaag aattccgccg
     1501 ccactggaac agcgccgaaa tcgaagtgct ctacgagcgc ctcaacgcta tcgtgcagcc
     1561 gctgcgattc tcccgtaacc tgaccttaca ggtcggcgcc aacgagttga ttcgcgacga
     1621 gcgcggcagc ggcgagccct ttgacgggac cgtcgaattc tggtggcaaa atgccggcga
     1681 cctgctgcaa aaatacgatt ccaatgaagc acggcgagtg cgcgaaagca tgctcgattt
     1741 gcaggagcga ttcatcgaca tgggaggatg ccgcgccttc tttaccgagt accccgacgc
     1801 acagccctga ggccctgatt acggcggcag gatgacgcgg tccagcttcg ctaccaggaa
     1861 cggcttgcag gccgagttga cgatctcggc ttcgccgcta agcaacaggc tttgcatcgc
     1921 caccagttcg tcgtcgtcga ggctatccag taacaggtag ccgccgccgc gcacgcgggc
     1981 gcgcaggcgc tcgagcgtat cgccagcgcg cgccgccggt tcatccactc ggtgtcgttt
     2041 ggtcatcagt gctcctcgct tatcgaagat tgcgacgcag actcgatttt ttccagccgg
     2101 gcacgaaatt cgtccctgaa atccgccgcc aggcgatcgc agagcacggc ctgccgcagg
     2161 ccctcgaggt tgcgtcgatc gaggtagtag agacgatgca cggcttccac cgtgggagct
     2221 tcgaggttcg cgtactcgag ctcgatggcg tcaccggcgc gaatttctcc ctcgcgcagc
     2281 acgcgcaggt aaaacccggt gcgcgcgctg ttgatgcaca ccgcgagcgc ctcggcaaca
     2341 cccatgcgaa tcgcgaactt gtaacacggc gaacgcggct gggagacttc gagcagcgca
     2401 ccgccggtac ggtaacggtc gccgatatgc acccgcgatt ccagcaatcc ctcggtggtc
     2461 aggttttcgc cgaactggcc gggtgcgtaa tcctgctgcc cgagctgctg ctggtaatag
     2521 ccgtagtgtt cgcaaggaaa cgcgtagacc gccttgtcga tgccgccatg gacgccggga
     2581 tcggcctgct ggtcgccgtc gatgttcagc ttgcgcgcca tcagcggcgc atcgctggcg
     2641 tccttgtaaa tgccggtcca cacccgttcg cccttgtgtt cgacgagcct tggcttgccg
     2701 acgttcaacg actgtatgcg catgactact gctcccggcg ccgttggacg gcggcctgta
     2761 cgatgcggta ccccaacctt accccgaatc gcgccgacgg tcgaatacgg gcgagcgcga
     2821 tcggcacggc tgtcagtcgg ccgcggtcga gcgctgcagg gtctgccagt cgcgaccaac
     2881 gccgaccggc gccgcggacg gttcgagcat gcccttgccc ttgatgatat cggcggcgcg
     2941 ctcggcgacc atgatcgtcg gcgaattcag gttgccgttc ggaatcgtcg ggaagatcga
     3001 cgaatcgacc acgcgcaaac cgtcgatgcc gcgcacccgg gtctcggaat cgaccaccgc
     3061 catgcggcag gcgcaggacg ggtggtaggc cgactcgacc gcctggcgca caaaggcgtc
     3121 gatttcggca tcgctctgca ccgcttcgcc gggttgaatc tcggggccgc gataggcatc
     3181 catcgccggc tggccgatga tctcgcgggt cagacgcacg caggcgcgaa acgcctcgat
     3241 atcgtcggcg tgttgcaggt agttgaagag gatttccggc ttgtcctgca cccgcggcgt
     3301 gcgcgcgcgc acatgaccgc ggctgttggg cttgttatgg ccgacgtgca cctggaagcc
     3361 gtgaccgtcg aatgccgcgc ggccgtcgta acgcatcgcg cccggcagga aatggtactg
     3421 gatatcgggc cactcgatgc ctgcccggga acgaatgaag gcacaggact cgaagtgatt
     3481 ggtcgcgccg agaccctttt tggtcagcac ccagcgcaaa ccgatcagga acttgcgcca
     3541 cggactgagt tcggcattca gcgtgatcgg ctggcggcag cgaaactgga agtaaaactc
     3601 gaggtgatcc tgcaggtttt cgccgacccc cggcagatcg tgcacaacct cgaggccggc
     3661 ccgctgcagc accgcgcccg ggccgatgcc ggacaactgc agcagctgcg gcgaggcgat
     3721 cgggccggcg ctgagcaaga cttcgcggcg cgccttcagt atctgcgtgc tgccggcgcg
     3781 caggaactcg acaccgaccg cgcgccgctg ctcgagcagg atgcgctgcg tcaacgcgcc
     3841 ggtgatcacg gtcaggttgg ggcgctgcaa cgccggcttc aggtaggcat tggcagtcga
     3901 ccagcgcacg ccgttgttga ccgtcatgtg catcggcccg aagccttcct gctggcggcc
     3961 gttgtaatcg gcggttttca tgtagccggc ctgctcgccg gcctcgatga aggcgcggta
     4021 gagcgggttt tgcatgttgt tgccattgtt gaccgcgagc ggcccgcccc gagcgcggta
     4081 ctcgtcgctg ccgaaggccc agtcgtcggc ctttttgaaa tacggcaggc aatggcggta
     4141 atcccagtcc ctggcgccct ggctttgcca ctcgtcaaaa tcgcgcgcat ggccgcgcac
     4201 atagaccatg ccgttgatcg acgacgagcc gcccagcacc ttgccgcgcg ggcagtgcat
     4261 gcgacggttg tcgagataag gctcgggttc ggactcgtag aaccagttgt acttcttcat
     4321 gttcatcggt atcgacagcg cggtcggcat ctggatgaag atgctgctgt cgctgccgcc
     4381 gaactcgagc agcgccacct tcaccgcgtg gtcctcgctg agccggttgg cgagcacgca
     4441 gccggccgaa ccggcgccga cgatgatgta atcgtattcc tggtccatgg ttctacccgt
     4501 tgacgtgagc cgcgatctta ttattttcgg ccgatattgt cttgatcgaa tttagcgctt
     4561 gacagaatct ttatatgtcg attattctcg acaaatggat attacgtctg caacccgcgc
     4621 gctcggcgcg ctctcccagg aatcgcgact gaaggcgttt cggctgctgg tgcagagcgg
     4681 cgccgacggc atcgccgccg gcaagatcgc cgaaaccctg gcgattccgc ataacaccat
     4741 gtcgacccac ctggcgacgc tggtcaacgc cggcctcgcg gtgtcgcggc gcgagagccg
     4801 ctcgatcatt taccgcgtcg atttcaacgg tacccgcgaa ttgttgtcgt tcctggtcga
     4861 ggattgctgc cagggtcggc ccgaagcctg cctgccggcg ctggaaagcg tgctgccggg
     4921 atgctgcgcc cccaaccaac cttccggagt caactcatga aacgcctgca cgtcaatgtc
     4981 tcggtcaccg atctcgaggc ctcggtcggc ttttacagcg ccctgttcga cgctgcgccg
     5041 acgctactca aaagcgatta cgcgaagtgg atgctcgaag acccgcgcgt caatttcgcg
     5101 attaccagcc gcggccagcg caagggcatc gaccatctcg gcatccaggt cgaggacgac
     5161 gccgagctcg acgaggttta cgcgcgcctg aaggcggccg acgcgccgac catcgagcag
     5221 ggcgaaacca cctgctgcta cgcgaactcg accaagagct ggatcttcga tcccgacagc
     5281 atcgcctggg aaaccttttt gacccgtggc gaaagcgcgg tctatggcag cgacgaaatg
     5341 cttgcgcgcg agcaggcctc ggcctgttgc agccgcccac aggcgaaaga caccgccggt
     5401 aattgctgct agcagcgcgc cgcgagatcg cgaatccgta gtccgcgcag ccagacgacg
     5461 aagttacgaa atgcccgagc aaaaggccaa cgtgctgttt ctgtgcaccg gcaattcggc
     5521 ccgcagcgtc atcgccgaga gcctgatcaa tcgcctcggt cacggtcgct tcagcggcta
     5581 tagcgccggc agccacccgg ccggcgacgt gcatccgctg gcccgctcgg tgctgaagaa
     5641 gaacgattac cccaccgacg gtctgcgcag caagagcctg gaagaattca gccggcccgg
     5701 ctcgccggtg atggacttcg tgttcacggt ttgcgaccgc gccgcggccg aggtttgccc
     5761 gctgtggccc ggccagccga tgagcgcgca ctggggcaca gccgacccgg ccgcggtcga
     5821 gggttcggaa atcgacagac gcgccgcatt ccgcagcact ttcgacgaac tgcatagccg
     5881 catctcgata ttcgtcaacc tgcccttcga tttcctcgac cggctcgagc tcaagcaaag
     5941 actcgacgaa atcggccgct ccaggccggc agactgagcg cttcgacggc gccgctgaag
     6001 acataaggac ttatctctca tgggaacttt cgaacgttat ctctcggtct gggtagccct
     6061 gtgcatcgcg gccggcgtga tcctcggtgt ggtgatgccg ggattcttcg aatcgatcgc
     6121 ggcgatcgag tatgcgagcg tcaacctggt ggtcgcggtg ttcatctgga tcatgattta
     6181 cccgatgatg gtcaacgtcg acttcgcttc gctaaaagac gtcggcaaga agccccgcgg
     6241 gctctgcatc acgctggtgg tcaactggct gatcaagccg ttcaccatgg cggcgctcgg
     6301 catcctgttt ttcgagtata ttttcgccgg cctggtcgag ccgcaaaccg ccaaggaata
     6361 catcgccggc atgatcctgc tcggtgtcgc gccatgcacc gcgatggtat tcgtctggag
     6421 ccagctggtg cgcggcgacg ccaactatac gctggtgcag gtgtcgatca acgacgtcat
     6481 catggtgttc gcattcgcgc cgctcgcggc cctgctgctg ggggttaccg atatcgtcgt
     6541 gccctgggaa accctgctgc tgtcggtggt tctatacgta ttgataccgc tcgcggcggg
     6601 ttacgccacg cgcaaaatgc tcgacggctc gaggaatcat gcccgcatcg agcagtttac
     6661 caccggcatc aagcctttct cggtcatggg gctgctggcg accgtagtgc tgctgttcgg
     6721 attccaggcc gaaaccatca tcgacaatcc cgcggtgata gccctgattg caataccact
     6781 gctgatccag agttatgcga ttttcgtcgt cgcttacggc tgggcatacc tgtggagggt
     6841 acccttcacc accgcagcgc cggccgcgct gatcggcaca tccaatttct tcgagctcgc
     6901 ggtcgcggtc gcgatcagcc tgttcggcct cgattcgggt gccgcgctcg ccaccgtggt
     6961 cggcgtgctg gtcgaggtgc cggtgatgct gtcgctggtc gccttcgcca accgcactcg
     7021 ccaccacttc gcctgagccg aacaaaaaag cccgcgcgaa cgcgggcttt cgattttcgc
     7081 aataccggct aaggcttcaa gaccagggaa acgggctatt cgaaaccggg tgcagcctgc
     7141 cctcggcata gcgcgaaaag cggaagggtt cgagcagcgc gctcttttcg ccgcacagtt
     7201 cctcggccac cagtttgccg acgccgagca tcttgtagcc gtggttggaa tcggcgatca
     7261 ggtagcaatt ctcgcggaac acgtcgaata ccggaaagct gtcgggcgta aagcagccga
     7321 tgccgcccga cggctcgttc ttgtagaact cgaactgtcc ctcgaagcgc ttctggcagt
     7381 gcgccagcgc cgaggtccac atgtgcgcga aatcctcgcc gacgacgaaa tcgggcgagt
     7441 cgacgccgta gggatcgacc gcgacatcgt ccgggtcgcg gtcgaccgtg taaggcgccg
     7501 cgcccccttg aataccgccg aagtgaaaat cgggcttgta gtagatgccc cacatcttgt
     7561 cggtgatcag gctgccgtcg aaatccgagt acagcggttc gttgctgtcg acgtggatca
     7621 ccggcggaaa cttgccgtcg ttggttttct gcagctccgg atcgacgccg agcgtgccct
     7681 cctgcagcga ccagtagatc cacatgggca cgttttcgtg gaccgtaccg tcggcaccct
     7741 tgatgctgat ggtcttcggc aactcgagca tgtcccagac agtcttcgcc cacggaccgg
     7801 cgccgatgac cacctggtcg cagccgatct cgccgcgatc ggtttcgacc gcgcgaatcg
     7861 caccgccggc cgagtcgctg ctgaagccgg tcaccgtgca gccggtcagg atacgcacgc
     7921 cctcggcctc ggccttgccg gcgagcgcgt acattgcttt cgtattgttg gcgtagccgc
     7981 cgcgcttctc gtgcagcacc gaggtaatgc cctttgcctg ccagtcgtgg aacaggtttt
     8041 tcatgtagcc catcgaatcc ttctcgccct cgatgaacac cgattcgtag ccgatctcct
     8101 tctgctgccg ggcgatactg gcgacatcct cgtgcatgct ctccgggctg atctgcatat
     8161 agccgaccgg gtggtagcta taaccctcgg gatcggtttc ccagacgccg acgctgtgcg
     8221 ccatcagttc gcgcatcgcc ggctgaaagt aattgttgcg caccacgccg caggcgatac
     8281 ccgaggcgcc ggcggcgatg ccgcccttgt cgaccaccag gatatcgcgg ccgtcgccgt
     8341 tgccggtcgc cttcagtttc tgcgccaggt gataagccgt gctgaggccg tgaataccgg
     8401 caccgattac gaggtacttc acgtgagaag gaatcgccat cgatcaatcc gccattaaat
     8461 tgagttgcca gaaccgggcc aacaccccgg aaacaggtcc aagcataacc aaaagcgccg
     8521 cggcgaacga cgctagaacg acctgacgac gaacaattac gacatgccgc aggccgggcc
     8581 acgctcgatc gcaacctccg ctacagggct tcaaccgcgg cacgaatgcg ctccacggcc
     8641 tgctcgacgc ggctgcgcgg gcagccaaaa ttgaggcgca tgaaattcgc gtcgccgaat
     8701 tcgaatcccg gcgacatgcc gacgccggcc tcctcgaaaa accgcggcgg ctgctcgagt
     8761 tgcagcgcgg agacgtcgat ccatgccagg taagtcgctt ccaccggtcc cagaccgagg
     8821 cccggaatgc ggttgatttc atcgaccagg taatcgcggt tggcggcgag gtaggcgcac
     8881 tgctggcgat tccactcgtc gccgtggcgg taggccgcct cggccgcgac cagccccgtg
     8941 atgctgacat ggggcaggat gaacgggttg gttttcatgc cccgccgcag cccggggtca
     9001 gcgacgatcg cgaatgcgca acccagcccg gcgaggttga aggtcttgct cggcgcaatc
     9061 agcgtgatcg tgcgctgctc gatgtcgcg
//
LOCUS       DATCZV010000410         3294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_246141, whole genome shotgun sequence.
ACCESSION   DATCZV010000410 DATCZV010000000
VERSION     DATCZV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246141"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VIW27_09305"
     CDS             <1..1087
                     /locus_tag="VIW27_09305"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY5739911.1"
                     /translation="RRIGVLTQYKAHSLIRHLVRGWSRFDSELDEFVEILPASQRAGG
                     EWYQGTADALYQNLDIMRTHQPEYVLILSGDHIYKMDYAEMIATHVDSKADMTIACLE
                     VPLEEAAGAFGVMTVNEAGRIIEFNEKPADPAPIPGEPGLCLASMGNYLFNTEFLYEQ
                     LIKDADDPKSDHDFGKNIIPSIIDKYQVYAFPFRDMDTGKRAYWRDVGTLDAFWEANM
                     ELVNVTPELNLYDQDWPILTHQAQLPPAKFVFDSDDRRGLAIDSMVSGGCVISGAQVT
                     RSLLFSNVHVHSYAEVTDSVILPEVEIGRNARIIRAIIDRGCTIDEGDVIGVDHDADR
                     KRGFRVTEKGVVLVTPDMLHQATHARR"
     gene            1094..2746
                     /locus_tag="VIW27_09310"
     CDS             1094..2746
                     /locus_tag="VIW27_09310"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEY5739912.1"
                     /translation="MNTRITTATRPYPDQKPGTSGLRKKVRVFQQPNYLENFVQSIFD
                     ATAGSKGPVAGQTLVVGGDGRYYNGQALQTIIRMAAANGFERIVAGRDGLLSTPAASC
                     VIRKYRAFGGIILSASHNPGGPDADFGIKFNAANGGPASEELTGAIYERSRKIKNYHS
                     LDSAADIDLAGIGKQRLDGLVVEVIDPVADYAEMMQRLFDFERISELLSAGDFRLQFD
                     AMNAVTGPYAHEILERRLGAPAGSVLRGTPLTDFGGDHPDPNLAHAPELLRRMRGADA
                     ADFGAASDGDGDRNMILGANFYVTPSDSLALLAANATLVPGYRDGLVGVARSMPTSQA
                     VDRVAGELGIPCYETPTGWKFFGNLLDAGKITLCGEESFGTGSDHVREKDGLWAVLFW
                     LNILAARGEPVAEIVRRHWRRYGRDYYSRHDYEGIDSDAANALVESLRQRVGGLAGQT
                     CGGQRIEFADDFAYSDPIDGSSSQRQGIRILLRDGARVVYRLSGTGTEGATLRVYIER
                     FEADPARQAEDPQAALRPIIEFASQLAEIEKFTGRSSPDVIT"
     gene            complement(3066..>3294)
                     /locus_tag="VIW27_09315"
     CDS             complement(3066..>3294)
                     /locus_tag="VIW27_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY5739913.1"
                     /translation="LMFDTSMSLELLFRGLATMDEAMAKVAGVTSLQLHLSDEARGLP
                     PIPEVPVPAAVWLFGTALIGFIGYSRKARAS"
BASE COUNT          628 a         1096 c         1011 g          559 t
ORIGIN      
        1 tcgccgcatc ggcgtgctga cccagtacaa ggcgcactcg ctgattcgcc acctggtgcg
       61 cggctggagt cgcttcgaca gcgagctcga cgaattcgtc gaaattctgc cggcctcgca
      121 gcgtgccggc ggcgaatggt accagggtac ggccgacgcg ctttaccaga atctcgacat
      181 catgcgcacc caccagcccg agtacgtgtt gatcctgtcg ggcgatcata tctacaagat
      241 ggattatgcc gagatgatcg ccacccacgt cgacagcaag gcagacatga ccatcgcctg
      301 tctcgaggta ccactcgaag aagcggccgg tgccttcggc gtgatgacgg tcaacgaagc
      361 cggccgcatc atcgagttca acgaaaagcc ggccgatcct gccccgattc ccggcgagcc
      421 cggcttgtgc ctggcgtcga tgggcaatta cctgttcaac accgagttcc tctacgaaca
      481 attgatcaag gatgccgacg atccgaagtc ggaccacgat ttcggcaaaa atatcattcc
      541 gtcgatcatc gacaagtacc aggtctacgc gtttccgttt cgcgacatgg ataccggcaa
      601 gcgcgcctac tggcgggatg tcggcaccct cgatgcattc tgggaagcca acatggaact
      661 cgtcaacgtg acccccgaat tgaacctcta cgaccaggac tggccgatac tgacccacca
      721 ggcgcagctg ccgcccgcca agttcgtgtt cgactccgac gatcgacgcg gcctggcgat
      781 cgattcgatg gtctccggcg gctgcgtgat ctccggcgcc caggtcacgc gctcgctgct
      841 gttttccaac gttcacgtgc attcctatgc cgaggtcacc gacagcgtaa tcctgcccga
      901 ggtcgaaatc ggccgcaacg cgcgcatcat ccgggcgatc atcgatcgcg gctgcaccat
      961 cgacgagggc gatgtgatcg gcgtcgacca cgacgccgac cgcaagcgcg gatttcgtgt
     1021 caccgagaaa ggcgtggtac tggtgacccc ggatatgcta caccaggcga cacatgcgcg
     1081 cagataacga gtcttgaata cgcgcattac caccgccacg cgcccgtacc cggatcaaaa
     1141 acccggcacc tcggggctgc gcaagaaggt gcgcgtattc cagcagccca attatctcga
     1201 aaactttgtg cagtctatct tcgacgcgac cgcgggcagc aagggcccgg tagcggggca
     1261 gaccctggtg gtcggcggcg acggccgcta ctacaatggc caggccctgc aaacgattat
     1321 tcgcatggcc gcggccaatg gattcgaacg catcgtcgcc ggccgcgatg gcctgttgtc
     1381 gacgccggcc gcatcctgcg taattcgcaa ataccgcgct tttggcggca tcatcctctc
     1441 cgccagccac aatccgggcg gccccgacgc cgatttcggc atcaagttca acgccgccaa
     1501 cggcgggccg gccagcgaag agttgaccgg cgcgatctac gagcgcagcc gtaagatcaa
     1561 gaattatcac agcctcgatt ccgccgccga catcgatctc gcggggatcg gaaagcagcg
     1621 gctcgacggc ctggtcgtcg aggtcatcga cccggtcgcc gactacgcgg agatgatgca
     1681 acggctgttc gatttcgaac gcattagtga gctgctgagc gcgggcgatt ttcggctgca
     1741 gttcgacgcc atgaacgcgg ttaccgggcc ctacgcccac gaaattctcg agcggcgcct
     1801 cggcgcaccc gccggtagcg tgctgcgcgg cacgccgctg acggatttcg gcggcgacca
     1861 cccggatccc aacctcgctc atgcgcccga gctgctgcgc cgcatgcgcg gcgctgacgc
     1921 cgccgatttt ggcgccgcat ccgacggcga cggcgatcgc aacatgattc tcggcgccaa
     1981 tttctacgtc acacccagcg acagcctggc gctgctggcc gccaacgcga ccctggttcc
     2041 cggctaccgc gacgggctcg tcggtgtggc gcgctcgatg ccgaccagcc aggcggtcga
     2101 ccgcgtcgcc ggcgagctcg gcatcccgtg ctacgaaacc ccgaccggct ggaaattttt
     2161 cggtaacctg ctcgacgccg gcaagatcac gctgtgcggc gaggagtcgt tcggcaccgg
     2221 ttccgatcac gtgcgcgaaa aggacgggct gtgggcggtc ctgttctggc tcaacatact
     2281 cgccgcgcgc ggcgagccgg tcgccgaaat cgtgcgccgg cactggcgaa gatacgggcg
     2341 cgactattac agtcgccacg actacgaggg tatcgacagc gatgccgcca acgcgctggt
     2401 cgaatccctg cggcagcgcg tcggcgggct ggccgggcag acttgcggcg gacagcggat
     2461 cgagttcgcc gacgatttcg cctacagcga ccccatcgac ggcagcagca gccagcggca
     2521 gggtattcgc atcctgctgc gcgacggcgc gcgtgtcgtt taccggctgt caggtaccgg
     2581 caccgaaggc gccacgctga gggtttatat cgagcgtttc gaagccgatc cggcacgcca
     2641 ggcagaggat ccgcaagccg ctctgcggcc gatcatcgag ttcgcctcgc agctcgccga
     2701 aatcgaaaag tttaccggcc gcagcagccc ggacgtgatt acctgacggc ccgggccgcg
     2761 atgggcgccg ccgctggcgg tttgcgcgct gaaatcgcgt cacgttaccc ctgggacttg
     2821 ctggcaaggc cgcttatgcc ggcctgattg gtggcgttgc atagttgcgc gcaggcgctg
     2881 ccgaagccgg tgatcgatgc ctgaccacga tatcgtaaaa cccgtcccgc ggagcggtaa
     2941 tcggcgcggc gtgcgaatcg ccggcggcaa aactcgatcg ccgcctcatg atcttcgacg
     3001 tcaccgtgga caccccgaca aacctcgcac gcaaggctta agcggctatc tcgcttgcgc
     3061 cggcatcagg acgccctggc tttgcgcgag tatccgatga agccgatcaa cgcggtgccg
     3121 aacaaccaga cagccgcagg caccgggacc tcggggatcg ggggcaaccc gcgtgcctcg
     3181 tccgacaggt gcagttgcaa agacgtcacg ccagcgacct tggccatggc ctcgtccatc
     3241 gttgcgagac cccggaacag cagctccagc gacatggagg tgtcgaacat cagg
//
LOCUS       DATCZV010000411         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_565041, whole genome shotgun sequence.
ACCESSION   DATCZV010000411 DATCZV010000000
VERSION     DATCZV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565041"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..268)
                     /locus_tag="VIW27_09320"
     CDS             complement(23..268)
                     /locus_tag="VIW27_09320"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEY5739914.1"
                     /translation="MSTMKAKHFHLIDDMDIAALTFAVIERVEAFVETVKRTLTSWIR
                     RSNERRELKAMSQHMLDDIGLTRHAIDREIAKYFWQK"
     gene            complement(648..1625)
                     /locus_tag="VIW27_09325"
     CDS             complement(648..1625)
                     /locus_tag="VIW27_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY5739915.1"
                     /translation="MTKAIRIHETGGPEVLRWEDIEVGDPGVGEVRLKQTACGLNYID
                     VYVRSGMYPISDFPAVIGMEAVGVVDALGDGVDNLAVGDRVAYPMVLGSYAEQRLIAA
                     EKLVKLPDSIADETAAAMMLKGLTAHYLLFRSYPVKPGDSILVYAAAGGVGLILCQWA
                     NLLGVNVIGCVGSEEKAELARANGCHHTIMYRDEDIAARVREITDGKGVAAAYDSIGK
                     ATFEASLDSLQPFGVLVTYGNASGPVEPFSPLILAGKGSLYVTRPTLATHVSTRALLE
                     EGANRLFDVVGNGQVVVNVNQALPLADCAEAHRQLEARETTGSTILTIG"
     gene            complement(1677..2546)
                     /locus_tag="VIW27_09330"
     CDS             complement(1677..2546)
                     /locus_tag="VIW27_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739916.1"
                     /translation="MIDLSERHRGMAFALLTACGLGAITTQAKLFYADGGNALTLMLA
                     RFIASVVVFGLLLAVGRRGFGIEPRLRLPLLALGLVWPGATICYLLAVETISVSLAVL
                     IFYFYPLLVLVYALLRRQLAASPALLGLFLGAFLGLYLALAGGAITFNTIGGGFAVLA
                     SVGAAFTFVCGARVAPAMSPLLMTFWINAAGLLLILPLMPGSFSLPAAGGWLAAGLAT
                     LLYLVAILSQFQALARLPAARAAFLLNLEPVVSILLAGIVLGEVLTPTQWIGVVLVVS
                     MIVLSLRFRPADA"
     gene            2759..>2960
                     /locus_tag="VIW27_09335"
     CDS             2759..>2960
                     /locus_tag="VIW27_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY5739917.1"
                     /translation="MSELQLPGSLVDVGWLHRHLDDDRLLVFDSSWHMPATGRDGYRE
                     WLEAHIRGARFFDFDGRIRDPDS"
BASE COUNT          626 a          875 c          914 g          545 t
ORIGIN      
        1 ggtgaggccc gcgccgcgcg gattacttct gccagaaata cttggcgatt tcccggtcga
       61 tcgcgtggcg ggtcagaccg atgtcgtcga gcatgtgctg gctcatagcc ttcagttcgc
      121 gacgctcgtt ggaacggcgg atccagctcg tcagcgtgcg tttgacggtt tccacgaatg
      181 cctcgacacg ctcgattacc gcaaaagtca gcgcggcgat atccatgtcg tcgatcaggt
      241 gaaagtgttt tgctttcata gtgctcatgt cgttgctcct atctcagggc cgtcggccga
      301 gaccggcggc gtatgtgtct tcagttgctg caaagtatag gagcggtcaa tgaaagtaac
      361 agattcagtt attttgaaat ttaaccatta cagtttttaa taacagattg gcacccaaat
      421 tgccgctgtg aagtggttca cagtaaaatt gtgcgctgca gcacagtgtt tttccagaca
      481 attccagagt aactttatga ggtattataa gttactgtta ttattcgagt tttattgaaa
      541 atcggagctg aaaaaataac cccgaaaagg tctcgtcaag gcgtatgcat gacgcccgaa
      601 gcaaattttc cgagcgtcat gcaaaactgt actggtagta cagtttctta gccgattgtc
      661 aggatcgtcg agcccgtggt ttcgcgtgct tcgagctggc ggtgagcctc cgcgcagtcc
      721 gccagcggta gcgcctggtt gacgttgacg accacctggc cgttgccgac cacgtcgaac
      781 agacggttgg cgccttcctc gagcagcgcg cgcgtcgaca cgtgggtcgc gagcgtcggg
      841 cgggtgacgt agagcgagcc cttgccggcc aggatcagcg gactgaatgg ctccaccggt
      901 ccgctggcgt tgccgtaggt gacgagtacg ccgaacggtt gcagcgaatc cagtgacgcc
      961 tcgaaggtgg ccttgccgat ggagtcgtaa gccgccgcaa cgcctttacc gtcggtaatc
     1021 tcacgcaccc gggcggcgat gtcctcgtcg cggtacatga tggtgtgatg acagccgttg
     1081 gcgcgcgcga gctcggcctt ttcctcagag ccgacgcagc cgatcacgtt gacgccgagc
     1141 aggttggccc actggcacag tatcagcccg acaccgccgg ccgcggcgta cacgaggatg
     1201 ctgtcgccgg gctttaccgg gtaggatctg aacagcaggt aatgcgccgt gaggcctttc
     1261 agcatcattg ccgccgcggt ttcgtcggcg atgctgtcgg gcagcttgac cagtttttcg
     1321 gcggcgatca ggcgttgctc tgcatacgag ccgagaacca tcgggtaggc gacgcgatca
     1381 ccgaccgcga gattatcgac gccgtcgccg agcgcgtcga ccacaccgac ggcttccatg
     1441 ccgatcacgg cgggaaagtc actgatcggg tacatgccgc tgcgaacgta gacgtcgata
     1501 taattcaggc cgcaagccgt ctgcttcaga cgcacctcgc cgacacccgg gtcgccgacc
     1561 tcgatgtctt cccagcgaag tacttcgggt cccccggttt cgtggatgcg tattgccttg
     1621 gtcatgtcga tgttctccgt taaaaaaacc gacgatgccc taatcggacc gcgggatcaa
     1681 gcatccgccg gccggaagcg caacgacagc acgatcatcg aaaccacgag taccacgccg
     1741 atccattgcg tcggggtcaa aacctcgccg agcactatgc cggcgagcag tatcgatacc
     1801 accggctcga ggttgagcag gaacgccgcg cgggcggccg gcaggcgcgc cagggcctgg
     1861 aactgactga gtatcgcgac caggtaaagc agcgtcgcga gccctgcagc gagccatccg
     1921 ccggcggcgg gcagcgagaa actgcctggc atcaacggca ggatcagtaa cagcccggcg
     1981 gcattgatcc agaaggtcat cagcaacggc gacatggccg gtgccacccg ggcgccgcaa
     2041 acgaaagtga acgccgcacc gacactcgcc agcaccgcga aaccgccccc gatcgtgttg
     2101 aacgtgattg cgccgcccgc aagcgcgagg tacaggccca ggaaagcgcc gaggaacagt
     2161 ccgagcagcg cgggtgacgc ggcgagctgg cggcgcaaca gcgcgtaaac cagcaccagc
     2221 agcgggtaaa agtagaatat cagcacggcc aggctaacgg agatcgtctc gacagccagc
     2281 agatagcata tggtcgcgcc cggccaaacc aggcccaggg cgagcagggg cagccgcaac
     2341 cgcggctcga taccgaaacc gcggcggccg acggccagca gcaggccgaa aaccaccacc
     2401 gaagcgatga agcgggccag catcagggtc agcgcgttac caccatcggc gtaaaacagt
     2461 ttcgcctggg tggtaatcgc tccgaggccg caggcggtca gcagcgcgaa tgccatgccg
     2521 cggtgacgtt cggacagatc gatcatagcg tcagcactat atcctgtata attcgccagg
     2581 cgggcaaaat cttgcgaaag cgatacgaat ttcagccagg gactcgaagc tggcgaaatt
     2641 cagcggtggt aaagtgggtg ccgaacgagc ggtagtctca gggcagccgc gtccggccgc
     2701 gcttgccagg cgcgacgcga ttcgatactt tgccagtccc gagcaacagg aaagcatcat
     2761 gagcgaactg caactccccg ggtcgctggt cgatgtcggc tggctgcacc ggcatctcga
     2821 cgatgatcga ctgctggttt tcgatagcag ctggcatatg cccgccaccg ggcgtgacgg
     2881 ctatcgggaa tggctcgaag cgcacatccg gggcgctcgc tttttcgatt tcgacggccg
     2941 cattcgggac ccggattcgg
//
LOCUS       DATCZV010000412         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_86847, whole genome shotgun sequence.
ACCESSION   DATCZV010000412 DATCZV010000000
VERSION     DATCZV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86847"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VIW27_09340"
     CDS             complement(<1..229)
                     /locus_tag="VIW27_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739918.1"
                     /translation="MGLVLLVLISGAFNVAAAANVWDYIVGFSIREVSLDVYEKGSTD
                     PEGILTQGSSLIPEFGLESKVNYFSDSNWGYK"
     gene            complement(537..1550)
                     /locus_tag="VIW27_09345"
     CDS             complement(537..1550)
                     /locus_tag="VIW27_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HEY5739919.1"
                     /translation="MKRHATLLLCLLAAACASTPGGDGARPDWIDGQSSEYPQALFLS
                     GQGSALTPQDAKDRARSELAKQFEVAVRERSQQAQTFSSQQTGDESVQSLEQRVSRQL
                     LTSTAKSLQGVQIAEQWHDQSSGQYHALAVLSRSRARLQFEQEIAALDDRSRQWLRRA
                     ENEDMLLKRAGCVQQAIAAQQQRSVVQSSLQVVDASGRGQPSVISLAELERSRDTLLS
                     RITLQPSASGAMASELSNLLSAATASAGFKVTDSTRGDYRLLAQALLDPVIEQDGWYW
                     IRGTLQLKLVNDAGNDIGVKRWELKASSTNVERARQRFLSDTDALLKDELRQVLLGFA
                     VKP"
     gene            complement(1547..>2838)
                     /locus_tag="VIW27_09350"
     CDS             complement(1547..>2838)
                     /locus_tag="VIW27_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739920.1"
                     /translation="RRDPKPVAALVLAALMLGQVACVGAIRHETDLELFLSSNRTAEA
                     LQAIEMRHRAPRNETVFLLDKAMLLRMQQQYAASNDAFEQAKEVIEELDAISLREQAA
                     AVTINDSMRSYLPPTFERALIHCFKAMNYLQLRQYDSARVEILQLDEFLKQQDDFRLP
                     FARYLSGLVFELNQEPDNALIAYRKAYESYRDGGRAVPSLLQQDLLRLTDYLDRADEH
                     RQFDEEFALVNWSRQQNFRARTHVVAILFNGLIPRKHSHEILAQSPTDGQMHRISTPF
                     YEQRQTRVYNAMLSSDSAADDSELFADLERHARAALEDAMPGIIARTVARVAIKNRAV
                     DEASDEQPLLGLALNAFAVLSEQADTRGWYTLPQEILVTRLELEPGRHDLELLLDGSL
                     RPGAGQNWQQIELEPGETVVFGLHWPESYSTLGRRYR"
BASE COUNT          543 a          844 c          842 g          609 t
ORIGIN      
        1 atttgtagcc ccagttacta tcggaaaaat agttgacttt gctctcgagc ccaaactccg
       61 gaatcaacga ggaaccctgg gtcaggatac cttcaggatc ggttgatccc ttttcgtaga
      121 cgtcaagcga tacctcccga atgctgaagc caacaatgta gtcccagacg ttagcggctg
      181 cggccacatt gaaggcgcca gaaataagca caagcagaac cagccccaca atgcgctgct
      241 tttctaaccg gctaaataat cggcattccc tgtgatttat cggggggcat gtaacagatt
      301 cgataaccgg gttaacatgc tgcatttttt acccctgatg cgattctgta tttcgaatga
      361 ttcggggaac ttcatccaac tcaataagat tttacaaacc tcatagcttg ctcttttgat
      421 agggatcaag cgatgaacga ctttgtcgag ctagaggttt agaccgattg gcctgcagat
      481 actcggttcg agtgccctgg gtatatcacc gccggtcgag gtgggcaatg cccttatcag
      541 ggcttgaccg caaaacccag caatacttgc cgcagctcgt ccttcaacaa ggcatccgta
      601 tcgctgagaa atcgctgccg cgctcgctcg acattagtcg acgacgcctt gagttcccag
      661 cgcttgacgc cgatatcgtt accggcatcg ttaaccagtt tgagttgcaa tgttccgcgt
      721 atccagtacc acccgtcctg ttcgatgacc gggtcgagca aggcctgcgc gagcagccgg
      781 taatcacccc gagtcgaatc ggtgaccttg aaaccggcac tggcggtcgc agcgctgagc
      841 agattgctca attccgacgc catcgcgccg ctcgcgctcg gctgcagggt aatcctgctc
      901 aacagcgtat cgcggctgcg ctcgagttcg gcgagcgata tcaccgacgg ctgaccgcgc
      961 ccgctggcat cgactacctg cagggaactt tgcacgacgc tgcgctgctg ctgtgccgcg
     1021 atcgcctgct gcacgcatcc cgcgcgcttg agtagcatgt cttcattttc ggcacgccgc
     1081 agccactgtc ggctgcggtc atcgagtgcg gcgatttcct gctcgaactg caagcgcgcg
     1141 cggctgcgtg acaacaccgc cagcgcatga tactgaccgc tagactgatc atgccactgc
     1201 tcggcgattt gcactccctg caggctcttc gcggtggacg tcagcagctg gcgcgatacc
     1261 ctctgctcca gcgattgcac gctctcgtcg ccggtctgct gactgctgaa agtctgcgcc
     1321 tgctggctgc gctcacgtac ggcgacttcg aactgcttgg ccagttcgct gcgcgcgcgg
     1381 tctttcgcgt cctgtggagt cagcgcgctg ccctggccgc taagaaaaag cgcctgcggg
     1441 tactcgctgc tttgaccgtc gatccagtcg ggtcgggcac cgtcgccgcc gggagtactg
     1501 gcgcaggcag cggcgagcag gcagagcagc agcgtggcat ggcgtttcat cggtatcgtc
     1561 tcccaagggt ggaataggat tcgggccagt gtaggccgaa tacaacggtt tcgcccggct
     1621 cgagttcgat ttgctgccag ttctggccgg cgccagggcg caacgagcca tcgagtagca
     1681 gttccaggtc gtgtcgcccc ggttcgagtt cgagccgcgt caccaggatt tcttgcggca
     1741 aggtgtacca gccgcgggta tcggcctgtt cgctcagcac ggcgaacgcg ttcaatgcga
     1801 gaccgagcag gggctgctca tcgctggcct cgtcgacagc ccggttcttg atggcgactc
     1861 gcgccacggt gcgggcgatg atgcccggca tggcgtcctc cagtgcggcg cgggcgtggc
     1921 gttcgagatc cgcgaatagt tccgaatcgt cggcggcaga gtcactgctg agcatggcgt
     1981 tgtagacgcg agtttggcgt tgctcgtaaa acggcgtgga aatgcgatgc atctggccgt
     2041 cggtgggcga ctgcgccaga atttcgtggc tgtgcttacg cggaatcagg ccgttgaaca
     2101 ggatggccac cacgtgcgtg cgcgcgcgaa aattttgctg cctcgaccag ttaaccagtg
     2161 cgaattcttc gtcgaactgg cgatgctcgt cggcgcggtc gagatagtcg gtcagccgca
     2221 ataagtcctg ttgcaacagg ctcggcacgg cacgaccgcc gtcgcgataa gactcgtatg
     2281 ccttacggta tgcgatcaga gcgttatccg gttcctggtt caactcgaag accagccccg
     2341 acagatagcg tgcgaacggc agccgaaaat cgtcctgctg cttgaggaat tcatcgagtt
     2401 gcagaatctc gacgcgggcg ctgtcgtatt ggcgcagctg cagataattc atcgccttga
     2461 aacaatgaat aagcgcgcgc tcgaacgtcg gcggcaggta actgcgcatc gaatcgttga
     2521 tcgttaccgc cgcggcctgt tcccgcagac taatggcatc gagttcctcg atgacctcct
     2581 tggcctgctc gaacgcgtca ttgctggccg cgtattgctg ctgcatacgc agtaacatgg
     2641 ccttgtccag caagaaaaca gtctcgttac gcggcgcgcg gtggcgcatt tcgatcgcct
     2701 gcagcgcctc ggcggtgcgg ttgctgctaa ggaaaagctc gaggtcggtt tcgtggcgga
     2761 tcgcaccgac acaggcgacc tggcccagca ttagagcggc caggaccagc gccgctactg
     2821 gctttgggtc cctgcgct
//
LOCUS       DATCZV010000413         6890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_669783, whole genome shotgun sequence.
ACCESSION   DATCZV010000413 DATCZV010000000
VERSION     DATCZV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669783"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1102)
                     /locus_tag="VIW27_09355"
     CDS             complement(218..1102)
                     /locus_tag="VIW27_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5739921.1"
                     /translation="MQLRILVAIAVLVYTPGLIAADDQQGTIGEAAQALPLFDAHMHW
                     KRPAWEPYPPEKVLSLMDANGVAMALVSSSPDDGTITLWQYAPRRIVPEMRPYNEQLG
                     PSNWTRGEGVGDYIENRVRQYPHEGIGEFHMHAVDPDDEPLMRRIVALAIEKNIPLHV
                     HSGHRPIEYLYSLDPRLTVIWAHAGMSEPPDVIEAMMARYATLHADTSYREWEILRGA
                     DGIDADWRRVLERFADRFMVGSDTWVNGQWASYDRLMATNRQWLAQLTPATAHNIAYR
                     NAERLFGRRIGRQLFGTR"
     gene            complement(1271..1765)
                     /locus_tag="VIW27_09360"
     CDS             complement(1271..1765)
                     /locus_tag="VIW27_09360"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY5739922.1"
                     /translation="MSENREIAECRREADRREVSWRSFVYGNFRPRRRRSRREEDGHR
                     ILFDWYEPRVLYLTLGVLLLSCADALFTLNLLNHGAAEANFFMARALEHGMDSFLAGK
                     ISLTGISLICLVAVVRRKFIGDYSVEHLLQFFFFAYVLVICYEIYIFEFVFELNIFSL
                     NPPE"
     gene            complement(2121..2378)
                     /locus_tag="VIW27_09365"
     CDS             complement(2121..2378)
                     /locus_tag="VIW27_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739923.1"
                     /translation="MNTLTDTPVAGRQLDLSVQPRAAAAAVAEIATGIATGVAPQMRK
                     WDSRRVKDLLVMVYAYGLLGFGIFFPLLLAAWAAFFVPESG"
     gene            2762..4609
                     /locus_tag="VIW27_09370"
     CDS             2762..4609
                     /locus_tag="VIW27_09370"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEY5739924.1"
                     /translation="MNLSVTPLDDEEIRHAARVVRRDAGLDSSAWFETIGLDDSERFG
                     GERAAYVCCYEPASNRTFSGIVHFASDSLHDWRHVEGAQARIMAEESAFACELARGDG
                     RFLEGLQRRGIDDPSRVLVEAWSAGNFGNPEENERRLAWGFCWLMNDAGDNPYARPLD
                     GLHPVFDLARREIVRVEDHGSLPLPPDPGPIRRDAQRIDLREISITQPDGASFEVDGY
                     RVRWHDWELQIGFSQREGLVLHDIGIRDGGRLRPIMRRASMAEMVVPYGDPRGANWRR
                     NAFDTGEIGIGAALDSLQLGCDCLGHIHYFDVFTHDWHGEPRRIGNAVCMHEEDFGMQ
                     WKYSVPSTGQVTVTRARRLVISSIATIGNYVYGFFWYFYLDGSIGVEIKATGIPFPSA
                     QANGGPSRYGRTVGTGIESHVHQHVFNFRFDMAVDGDRNSASEVNFSAPPVGADNPHG
                     NAILTEETRFATELEAQREVDARQARYWRVLNPNVHNRYGQPVAYKLLPGNNAFAFQH
                     PDSAVGRRAGFMYKHLWVTPWAPDERYPAGWFPNQHAGGDGLPRWTRADRPIHDRRIV
                     VWHTLNYHHWPRPEDWPVQPVVFAAFHWMPDGFFDENPTMDLPRREIEE"
     gene            4888..6066
                     /locus_tag="VIW27_09375"
     CDS             4888..6066
                     /locus_tag="VIW27_09375"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY5739925.1"
                     /translation="MPFEAIFTLTVWGYLGIALLLTHVTILSVTIFLHRHQAHNALSL
                     NPAVSHFFRCWLWLTTGTVTREWVAVHRKHHAYCETEQDPHSPRYKGILRVLFGGLWL
                     YRREARNPETLQRFGQGTPDDWLERHLYGRHPWLGILIMLIAEVMLFGLPGLVIFAVQ
                     MVWIPFWAAGVINGLGHYLGYRGFETADASRNILPWGILVGGEELHNNHHAYAASARL
                     SCHWWEIDIGWYYIRLLAILRLARIKRLAPRAISTRGRQLIDHDTVRAVVSQRFYILQ
                     RYGKIVIRPALREARRNTDRVGRRLIQRARNLLTREDIAPDHEARETIARVLQQDPTL
                     ATIYHFKQQLKEVWSRTGDGQPGRVERLQAWCAASEQSGIRALEEFAQFLRGYRLQSA
                     "
     gene            6162..6482
                     /locus_tag="VIW27_09380"
     CDS             6162..6482
                     /locus_tag="VIW27_09380"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY5739926.1"
                     /translation="MKRITRFSLLTLLWALTAPVQAAMVATPHIGMESTIPQTPAVTE
                     LRQQTVQQLIESGVDADRAEHRVAQMTDSQVLQLQGEIDALPAGGMSTTNLLLIIILI
                     ILIV"
BASE COUNT         1313 a         2198 c         2150 g         1229 t
ORIGIN      
        1 gtagggtccg ccgacgacgg gcagatcttt gcggtagaca tgaatttcgc tgatgcgcat
       61 ggcaggcacc ccgagctgat tgccgcgcca gtatagcgga ttgcccggct ggcggctgtc
      121 gctccgaagg cacaagaata atggcagttt tacctattcc tgatccgctc gatcgccacc
      181 atcgacagga tcgacacggc ccgcgcgttc gccgcgccta gcgcgtaccg aacagttgcc
      241 ggccgatcct gcggccgaac aggcgctcgg cgtttcggta ggcgatgttg tgcgcggtgg
      301 cgggcgtcag ttgcgcgagc cattgccggt tggttgccat caggcggtcg tagctcgccc
      361 actggccgtt gacccaggta tccgagccga ccatgaaacg gtccgcgaag cgctccagca
      421 cgcgccgcca gtcggcgtcg atgccgtcgg cgccgcgcag gatctcccat tcgcggtacg
      481 aggtgtcggc atgcagcgtc gcatagcggg ccatcatcgc ctcgatcacg tccggtggtt
      541 ccgacatgcc ggcgtgcgcc cagatgaccg tcaggcgtgg atcgagcgag tacaggtact
      601 cgattggccg gtggccggaa tgcacgtgca gcggtatgtt tttctcgatc gccagcgcga
      661 cgatgcggcg catcagcggc tcgtcgtcgg gatcgactgc gtgcatatga aattcgccga
      721 tgccctcgtg ggggtattgc cgcacgcgat tttcgatgta gtcgccgacc ccttcgccgc
      781 gcgtccagtt ggatggcccg agctgctcgt tgtagggacg catttccggt acgatacgcc
      841 gcggcgcgta ttgccacagc gtaatcgtgc cgtcgtccgg cgacgacgag accaaagcca
      901 tcgcgacgcc gttggcgtcc atcagcgaca gcaccttctc cgggggatac ggctcccagg
      961 cgggacgctt ccagtgcatg tgggcatcaa acagcggcag cgcctgcgca gcctcgccga
     1021 tcgtgccctg ttggtcatcg gcggcaatca accccggcgt gtacaccagg acggcaatgg
     1081 cgacgagtat tcgaagctgc atcctcgaga cctccgcagc agcgattcat ggcccggtat
     1141 tatatccgaa taccggtcga tgcaatggcg ttactttgct gatcccggaa taccgaaggg
     1201 taaacggcag cggctttcgg cgcggctcga gtcgcgaccg acggcgagat aatcgcttgc
     1261 cgcgcggtat ttattcaggc gggttgagtg aaaaaatatt caactcgaat acgaactcga
     1321 agatatagat ctcgtaacag atcaccagca cgtaggcgaa gaagaagaac tgcagcaggt
     1381 gctcgacact atagtcgccg ataaacttgc gccgcaccac cgccaccagg cagatcagcg
     1441 aaatcccggt caggcttatc ttgccggcga ggaaactgtc catgccgtgt tctagcgccc
     1501 tggccatgaa gaagttcgcc tcggcggcac catggttgag caggttcagc gtgaacagcg
     1561 catcggcgca gcttagcagc agcaccccca gcgtgaggta gagtacgcgc ggctcgtacc
     1621 agtcgaacag tatgcgatgc ccgtcttcct cgcgacggct gcgccggcgc cgcggccgaa
     1681 aattaccgta gacgaaggag cgccagctca cctcgcgacg gtccgcctcg cggcggcatt
     1741 ccgcgatttc gcggttttca gacatttgac ccctcgacca acagccctcg acgggcgaag
     1801 tgaatacggc acccgtggct gccgcggcgg tgtcagcaac ccgcggtcca gcaagggaaa
     1861 ggctgctgac gacgcgaata ctgattagtt aagtatagac acaagaagcc aatgctgggc
     1921 gtgcgccaac ggtaaaaaat tcacgcaccg tgtaattcgc aacacgaccg ggtagcgaca
     1981 accgccgtcc gaacccgttg ccaggcgcga cagtcagctt tgctgcggcg aagctggcag
     2041 ctgcgcactg gcggtttgcc tcgccgacgt ccggcgcttg ccgcggcgga ccgcgacagc
     2101 agcgacggcc ggcggttgcg tcagcctgat tctggcacga aaaacgccgc ccaggcggcc
     2161 agcagcagcg ggaaaaatat accgaacccg agcaggccgt aggcgtagac catcaccagc
     2221 aggtccttga cgcgccgcga gtcccacttg cgcatctgcg gagcgacgcc ggtggcgata
     2281 ccggtggcaa tctcggcgac ggccgcggcg gctgcccggg gttgaacgga aagatcgagt
     2341 tggcgacccg caacgggggt atccgtcagg gtattcattg aaggccaggc ctccgaacaa
     2401 agagagccgc aacagcgcgg cccatgaatt gtgcgccttc cacggcaaaa aacaagatgc
     2461 cgccgagaat aattttttta catgaaatac agggatttac acccgcctgg cacgctactg
     2521 agcgcgtttt ccgacaggtt tgggtgctcg accctgcggc cattcgcgag cggcggcacc
     2581 aggtctgtac cggcacggcg gcggccagtt tgtgcaccgc gcaccacgct gatccatgag
     2641 tgccgagttc gacgcgcgat tgcgatcaaa gctgctggtg tcgagtaact cagcgcagcc
     2701 ggcttggtat tttttctcag ccgggtaata tcgaggcagg caaccgcgcg gggagaaaaa
     2761 tatgaatctg tcggtgacac cgctggacga cgaagaaatt cgacacgccg cccgcgtggt
     2821 gcgacgcgac gccggcctcg actcgtcggc ctggttcgag accatcggtc tcgacgactc
     2881 ggagcgcttc ggcggcgaac gcgccgccta tgtatgctgc tacgaaccgg cgagcaatcg
     2941 taccttcagc ggtatcgtgc atttcgcctc cgacagcctg cacgactggc gccacgtcga
     3001 gggtgcgcag gcgcgtatca tggctgagga atcggcattc gcctgcgaac tggcgcgcgg
     3061 cgacggacgt ttcctcgagg gcctgcagcg gcgcggcatc gacgacccgt cacgggtgct
     3121 ggtggaggcc tggtcggcgg gtaattttgg taatcccgag gagaacgagc gacgcctcgc
     3181 ctggggcttc tgctggttga tgaacgacgc tggcgacaac ccctatgcgc ggccgctcga
     3241 cggcctgcac ccggtattcg atctcgcgcg gcgggaaatc gtgcgcgtcg aggatcacgg
     3301 cagcctgccg ctgccgccgg accccgggcc gatccggcgc gacgcgcagc gcatcgatct
     3361 gcgcgagatc tccatcaccc agcccgacgg cgcgagcttc gaggtcgacg gctaccgggt
     3421 tcgctggcat gactgggaac tgcagattgg cttttcgcaa cgcgagggcc tggtgctgca
     3481 cgatatcggc atccgtgacg gcggccgcct gcgcccgatc atgaggcgcg catcgatggc
     3541 cgaaatggtc gtgccctacg gcgacccgcg tggcgccaac tggcggcgca acgccttcga
     3601 caccggcgag atcggcatcg gcgcggcgct cgattcgctc cagctcggct gcgactgcct
     3661 cgggcacatc cattatttcg acgttttcac ccacgactgg cacggcgagc cgcggcgcat
     3721 cggcaacgcg gtgtgcatgc atgaggaaga cttcggcatg caatggaagt actcggtacc
     3781 ctcgacggga caggtcacgg taacccgcgc gcggcgactg gtgatttcat cgatcgctac
     3841 catcggcaac tatgtttacg gttttttctg gtacttctat ctcgatggca gcatcggcgt
     3901 cgagatcaag gcgaccggga ttccgtttcc gtcggcccag gcaaacggcg gtccgagccg
     3961 gtacggccgc acggttggta cgggaatcga gtcgcacgtg caccagcacg tattcaattt
     4021 tcgcttcgac atggccgtcg acggcgatcg caactcggcc agcgaggtca atttctcggc
     4081 gccgccggtg ggcgccgaca atccccatgg caatgcgatc ctgaccgagg agacgcggtt
     4141 cgcaaccgag ctcgaggcgc agcgtgaggt cgatgcgcgg caggcgcgct actggcgagt
     4201 gctgaacccg aacgtgcaca accgttacgg ccagccggtg gcttacaagc tgctgcccgg
     4261 caacaatgcc ttcgcattcc agcatcccga ctcggcggtg ggccggcgcg ccggtttcat
     4321 gtacaagcac ctgtgggtca cgccatgggc gcccgacgag cgttatccgg ccggctggtt
     4381 tccgaaccag catgccggcg gcgacggcct gccgcgctgg acgcgggccg accgcccgat
     4441 ccacgatcga cgtatcgtgg tctggcacac gctcaattat caccactggc cgcgccccga
     4501 ggactggccg gtgcaaccgg tggtattcgc cgccttccac tggatgccgg acggcttttt
     4561 cgacgaaaac ccgaccatgg acctgccgcg acgcgaaatc gaggagtgac atgaaccggt
     4621 tggccagcgc cgtcctgccg gcggtttgga gcctgacgac cgcgattccc cggcgaagct
     4681 ggccattggc gcccgcggct ggcaacatgc ggtgaaaaat ctgcccccgc aagcgatttt
     4741 cgagtaaact ctgcgctgca aacgagccgc cgaagagcgg caacaccaat ccctaaatat
     4801 gtgtgattga gcactgcgcg aacttgataa gcctgctatc caggttgttt cagagatatg
     4861 aagtcggttt agacaagagg tagcgtaatg ccattcgaag ccatattcac cctgacggtc
     4921 tggggttacc tgggtattgc cctgttgctg acccacgtga ccatcctcag cgtcaccata
     4981 ttcctgcacc gccatcaggc gcacaacgcg ctctcgctga atcccgcggt cagccatttt
     5041 ttccgctgct ggctgtggct gaccaccggt accgtaaccc gcgaatgggt tgcggtacac
     5101 cgcaagcacc acgcctattg cgaaaccgag caggatccgc acagccctcg ctacaagggc
     5161 attctgcgcg tgctgttcgg cggactgtgg ctatatcgcc gcgaggcccg caatcccgaa
     5221 accctgcaaa ggttcggcca gggcacgccc gatgactggc tggagcggca cctctacggc
     5281 cgtcatccgt ggctcggtat cctgatcatg ctgatcgccg aagtcatgct gttcggcctg
     5341 ccgggtctgg tcatcttcgc ggtgcaaatg gtgtggattc cgttctgggc ggccggtgtc
     5401 atcaatggcc tcgggcatta cctcggttac cgcggcttcg aaaccgccga tgcgtcgcgc
     5461 aacatcctgc cctggggcat cctcgtcggc ggcgaggaat tgcacaataa ccaccacgct
     5521 tatgccgcgt cggccaggct ctcgtgccac tggtgggaga tcgacatcgg ctggtactac
     5581 attcgcctgc tggctatttt gcgcctcgca cgcatcaagc gactggcgcc gcgcgcgatc
     5641 agcacccgcg gccgccagct gatcgaccac gacacggtac gcgcggtcgt cagccagcgt
     5701 ttctatattt tgcagcgcta cggaaagatc gtgattcggc cggcactgcg cgaggcgcgg
     5761 cgtaacaccg accgcgtcgg gcgccgcctg atccagcgcg ccaggaacct gctgacgcgc
     5821 gaggacattg cgccggatca cgaggccagg gagaccatcg cacgagtcct tcaacaggac
     5881 cctactctgg cgaccattta ccatttcaaa cagcaactca aggaagtctg gtcgcggacc
     5941 ggcgacggtc agcccggacg ggtcgagcga ctacaggcct ggtgcgcggc gtccgagcaa
     6001 agcggtatac gcgcactcga ggaatttgcg caattcctgc gcggctaccg cctgcaatcc
     6061 gcctgagaat tcgcccggcc gcaggtttgc gaatcgccga ttcttgacta tcctcggttg
     6121 atgcgaccgc aatacagtgg cgctcccagc gaggaaatta gatgaaacgc ataacaagat
     6181 tcagcctgct gacgctgctc tgggccctga cggctccggt acaggccgcc atggtggcga
     6241 ctccccatat cggcatggaa agtaccatcc cgcaaacccc agcggtgacc gaactgcgcc
     6301 aacaaaccgt gcaacaactg atcgaaagtg gcgtggacgc agaccgggcc gagcaccgcg
     6361 tcgcgcagat gaccgattcg caggtcctgc aactgcaggg tgaaatcgat gcgctgccgg
     6421 ccggcggcat gagcaccacc aacctgctgc tgatcatcat cctgattatc ctgatagtct
     6481 gaattgatcg ccgggggctc actccgccgc gcgaagggcc gtgcgaaagc gcgctcgagc
     6541 caccggtttt acactggcgg caatcgccct gtagcgcgcg aggtaagccg cgagacctag
     6601 cgacagcttg tccggaagcc gttcgacgtc gtcactggcg gcgaatcagc cgccagctgc
     6661 cgtcgccttg gaccgcgcat gctcgacagc tgaatacgcg cgctgaatcc gcgtcgcgtc
     6721 gcgccggcgg attggttaaa ctgcctgcca ccgtcaagca acgccggaca acaccgcatg
     6781 tgcaggaatc cacactccag tatccctctc catcgctttg acgcggccgc caccaagcgg
     6841 ggctggtcgt gatgcccggg ctgcgcgcgc tcgccgcgga cctgcgtgag
//
LOCUS       DATCZV010000414         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_35096, whole genome shotgun sequence.
ACCESSION   DATCZV010000414 DATCZV010000000
VERSION     DATCZV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35096"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(516..740)
                     /locus_tag="VIW27_09385"
     CDS             complement(516..740)
                     /locus_tag="VIW27_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739927.1"
                     /translation="MNADSKQLADKVVNSFRDLLDSDTRAAVGDANLSALRGMVSEVI
                     TERSKTIFDRLQQDLKQIETELVERVPLEL"
     gene            880..1992
                     /locus_tag="VIW27_09390"
     CDS             880..1992
                     /locus_tag="VIW27_09390"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY5739928.1"
                     /translation="MENVFTLADDLGPLKVIHVHEPGCGLQGVLVIDNVARGPSIGGV
                     RMAEDATTEECARLARAMTLKNAAADLPHGGGKSVLIGDPKMPREQKQLLIRGFACAL
                     REVEQYIFGPDMGTDEECMAWVKDEIERAVGLPREAGGIPLDEIGATGFGLLHAIEVA
                     REFCDIDIGNARIAIQGFGAVGGHAAKFLGAAGATAVAVADSTGTIYDPDGLDIDRLL
                     ALKREGRSVTEYDGGERRDGDAIIDVECDIWIPAARPDVIHEANVERLNTRLVVQGAN
                     IPVTDAAEKILHQKGILNIPDFIANAGGVICAAVEYQGASEAAALAAIEEKLRRNTHQ
                     VLEASRQQQITPRAAALARARARVEQAMRHRRWHIF"
     gene            2006..2941
                     /locus_tag="VIW27_09395"
     CDS             2006..2941
                     /locus_tag="VIW27_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY5739929.1"
                     /translation="MFRIRFHGRGGQGVKTASRILGTAFFEAGYEVQDAPRYGAERRG
                     APIFAYVRADRATINERGIITNPDLVVVADDTLVGMPAAGVTQGLNERCVLLINSLES
                     GETWRRRLNTQATVLVLETAEEVEDRAGLPYIGSRCAAAAAALTGVISRDQLLRALRQ
                     ELDYLPADVVRKNHDIALQVFTGMHSSAGIVTPGSMTSASDYSRPAWIDLPFEKAAIS
                     NLAIHAGATSVEVRTGLWRTMRPVIDYDKCNACAWICSTYCPDGAINVRADGYPDIDY
                     DHCKGCLICVAQCPPHAIESVAENTARARQPGATA"
     gene            2938..>3290
                     /locus_tag="VIW27_09400"
     CDS             2938..>3290
                     /locus_tag="VIW27_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HEY5739930.1"
                     /translation="MSGKLLTGNSAAAWGARLAGVDYIPAFPITPQTEIIEDLAAWID
                     RGEMDARLVTLESEHSMITAAGSAASSGVRAFTATSSQGLLYGMEMIYTVAGWRAPFV
                     LVNVSRGLASPITLES"
BASE COUNT          649 a         1033 c         1036 g          572 t
ORIGIN      
        1 cctcgggact gcgaaatacc atgtcgggaa agggttcatc gagacgatcg aagctttcgg
       61 ctggcgggct ggcggccgac agatcgagtg acaacaacag cagcggcagg gacagtagat
      121 agcgcatgac gggcctcgaa agattggcga cgaatcaggt actcgaaaca agcggacctg
      181 tttcgaatgc caaagttcgt tcgagtttct cgattggcag gataaaaccg gctgcccggg
      241 ataatcgagt cgcccgcatc agccgcggag cgctttggtc atcgggcacc cgcaaatact
      301 tgcgcctgcc gtccatcgat cgaaataccc tctcaagacc tcgctcgaat gtctgcccag
      361 ggatgcagtt tatgccaatt atccaccggg tcggaactca ggcgacaatc ggcaaccgcc
      421 ggggttcggg actcctgccg gccggcaacc ccagttcgac tcgtgcgccg tccagcccga
      481 cttgccgaag attgggatca accgctgcgc tcgcttcaca gctccagcgg gaccctctcc
      541 accaattcgg tctcgatctg cttcagatcc tgctgcagcc ggtcgaagat ggttttcgag
      601 cgctccgtta tcacttcact gaccatgcca cgcagcgcgc tcaggttggc gtcgccgacc
      661 gccgcgcgcg tgtcgctgtc cagcaggtcc cgaaacgagt tgaccacctt gtcggccagt
      721 tgttttgaat cagcgttcat gtttataccc ctgggtcgaa tattcgcttc cgtgaacact
      781 attaggcagg ggtaagcttg caactttgat caggatcaac gtagcaaaat tttcagcata
      841 caaaatgaat ctgtaatcgc cctgcatcga ggaaaacgta tggaaaatgt attcacgcta
      901 gccgatgacc tggggccgct caaggtgatc cacgtacacg agcccggctg cggcctgcaa
      961 ggcgtgctgg tgatcgacaa tgttgccagg gggccatcga tcggcggcgt gcgcatggcc
     1021 gaggacgcga ccaccgagga gtgtgcgcgg ctggcgcgcg cgatgacctt aaaaaatgcc
     1081 gccgccgacc tgccgcacgg cggcggcaag tcggtactga tcggtgatcc gaaaatgccg
     1141 cgcgagcaaa aacaactgct gattcgcggt ttcgcctgcg cactgcgcga ggtcgagcag
     1201 tacatattcg gtcccgacat gggtaccgac gaggaatgca tggcctgggt caaggatgaa
     1261 atcgagcgcg ccgtcggcct gcctcgcgag gcgggcggta taccgctgga cgagatcggc
     1321 gccaccggct tcggcctgct gcatgcgatc gaggtcgccc gggaattttg cgacatcgac
     1381 atcggcaatg cccgtatcgc gatccagggc ttcggcgcgg tcggcggtca cgccgcgaaa
     1441 ttccttggcg ccgccggcgc caccgcggtc gccgtggcgg acagcacggg caccatctac
     1501 gaccccgacg gcctcgatat cgaccgcctg ctggcattga aaagagaagg caggagcgtc
     1561 accgagtacg acggcggcga gcgccgcgac ggcgatgcga ttatcgatgt cgaatgcgac
     1621 atctggattc cggcggcaag acccgacgtc attcacgagg ctaacgtgga gcgcctgaat
     1681 acccgcctgg ttgtgcaggg agccaacatt ccggttaccg acgcggccga gaaaatcctg
     1741 catcagaaag gcattctcaa cattcccgac ttcatcgcca acgccggcgg cgtcatctgt
     1801 gccgcagtgg agtaccaggg cgccagcgag gccgctgccc ttgccgcgat tgaggaaaaa
     1861 ctgcggcgca atacgcacca agtgctcgag gcctcccggc agcagcaaat cacgccgcgc
     1921 gccgcggcgc tggcgagggc acgcgcccgg gttgagcagg cgatgcgcca tcggcgctgg
     1981 catatcttct aggcgggcgc agaccatgtt tcgaattcga tttcatggtc gcggcggtca
     2041 gggagtcaag actgccagtc gaattctcgg caccgccttt ttcgaagccg gctacgaggt
     2101 acaggacgca ccgcgctatg gcgccgaacg ccgcggcgct cccatttttg cctacgtccg
     2161 cgcggaccgg gcaaccatca acgaacgcgg gattatcacc aatcccgacc tggtggtggt
     2221 cgccgacgac accctggtcg gcatgcccgc ggccggggtt acccagggcc tgaacgagcg
     2281 ttgcgtgttg ctgatcaaca gcctggaaag cggcgaaacc tggcgccgaa ggctcaacac
     2341 gcaagccacg gtcctggttc tggagacggc ggaggaagtc gaggatcgcg ccggcctgcc
     2401 ctacatcggc agccgctgcg cggccgccgc ggcggcactg accggcgtta tttcgcgcga
     2461 ccaactgctg cgggccttgc gacaggaact cgattacctg ccggccgacg tggtccgtaa
     2521 aaaccacgat atcgcgctcc aagtgttcac cggcatgcac tccagcgcgg gcatcgtgac
     2581 accggggtca atgacgtcgg ccagcgatta cagcagaccc gcgtggatcg atctgccgtt
     2641 cgagaaggcg gcgatatcca acctggcgat acacgccggc gccaccagtg tcgaggtgcg
     2701 taccggcctg tggcgaacca tgcggccggt gatcgattat gacaaatgca atgcctgtgc
     2761 ctggatttgc agcacctact gccccgacgg cgcgatcaat gtgcgcgctg acggctaccc
     2821 cgacatcgac tacgaccact gcaagggctg cctgatctgc gtcgcgcagt gcccgccgca
     2881 cgcgatcgaa tcggtggcgg aaaacaccgc ccgtgcgcgg caacccggag ccacggcatg
     2941 agcggaaaat tactcaccgg caatagcgcg gccgcgtggg gcgcccggct cgcgggggtc
     3001 gactacattc cggcttttcc gattaccccg cagaccgaga tcatcgagga cctggcggcc
     3061 tggatcgacc gcggcgaaat ggatgcgcgc ctggtgacgc tcgagtccga gcactcgatg
     3121 attaccgcgg ccggcagcgc ggcgtcgagc ggggtgcggg cgtttaccgc cacctcgagc
     3181 cagggcctgc tgtacggcat ggagatgatt tataccgtgg cgggctggcg cgcgccattc
     3241 gtgctggtca acgtttcgcg cgggctggcc tcgccgatca cgctggaatc
//
LOCUS       DATCZV010000415         3151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_565594, whole genome shotgun sequence.
ACCESSION   DATCZV010000415 DATCZV010000000
VERSION     DATCZV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565594"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VIW27_09405"
     CDS             complement(<1..452)
                     /locus_tag="VIW27_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY5739931.1"
                     /translation="MFRTWLAALAMSMMLVPFGAGAQMAFVAGTDYENISSPVDTGQP
                     DKVVVTEVFWYGCPHCFRFEPYIERWSANLPDGVVFELLPSSLNPRWTEHARAYYSFQ
                     IMGVLEQVHRNFFDAIHLDRKKLTNLDSIAGFVADQGLDEKAFREHYFS"
     gene            complement(562..1170)
                     /locus_tag="VIW27_09410"
     CDS             complement(562..1170)
                     /locus_tag="VIW27_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5739932.1"
                     /translation="MKASHLVAMVLVLSGLSSFAATAADIEAGKAKSAICAACHGADG
                     NSSNPAWPSLAGQHASYIYKQLIDFKEGRRSNATMTGMVVTLSDDDMKNLAAFYQSQQ
                     AKGVAFDGELIDKGENIYRGGITDASVAACIGCHSPSAKGNGPAGWPALVGQHPEYIV
                     AQLQAFKQGMRANDPGNMMRNVSARMSEMEMKSVAAYIAGIR"
     gene            1261..1863
                     /gene="yihA"
                     /locus_tag="VIW27_09415"
     CDS             1261..1863
                     /gene="yihA"
                     /locus_tag="VIW27_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY5739933.1"
                     /translation="MHISFSKARFLTSAFELDQLPPDHGAEVAFAGRSNAGKSSAINC
                     LVGQKNLCKTSKTPGRTQLINFFELTPDSRLVDLPGYGFAKVPKTLRAHWDRVLSTFL
                     LERAALRGLVIVVDIRRGLGELDQALIDMIGARLPLHILLTKADKLSRSAARKAMIET
                     ERRLPGPAHSLSSLSVLSREGIDPLEQRCRSWLEAEELGE"
     gene            complement(2578..>3151)
                     /locus_tag="VIW27_09420"
     CDS             complement(2578..>3151)
                     /locus_tag="VIW27_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY5739934.1"
                     /translation="AAEVFGASLDEVSAEQRRAAKAINFGLIYGMSAFGLGRQLGIGR
                     NEAQQYVDTYFERYPGVRRYMDDTKERAREQGYVETVFGRRLYLPDINARNGNLRQYA
                     ERTAINAPMQGSAADIIKRAMIEVHRWLSARDDCQMIMQVHDELVFEVNAATVDDCRE
                     RIAEIMTGAADLSVALEVDAGVGQNWNEAH"
BASE COUNT          609 a          959 c          944 g          639 t
ORIGIN      
        1 gaaaaatagt gctcgcgaaa ggctttctcg tccagcccct ggtcggcaac gaagccggcg
       61 atgctgtcga ggttggtgag ctttttgcgg tcgaggtgaa tggcatcgaa aaaattacgg
      121 tgcacctgct cgagcacgcc cataatctgg aatgagtagt aagcgcgcgc atgctcggtc
      181 cagcgcggat tgagcgatga cggcagcagt tcgaagacca caccgtcggg caggttcgcc
      241 gaccagcgct cgatataggg ttcgaaacgg aaacagtgcg ggcagccgta ccagaaaact
      301 tcagtgacca cgaccttgtc gggttggccg gtgtcgaccg gggacgagat attctcgtaa
      361 tcggtgcccg cgacgaacgc catctgcgcg cccgcgccga acggcaccag catcatcgac
      421 attgccaatg ccgctagcca agttctgaac atgctgtttc tcctgaattg atttgcgcca
      481 atgaagacca tcgccggttc gaaaagttta gtcgattatc gccgcgcggg ggtgaattcc
      541 cgctgaacgc acgggcgggt gctagcgaat gccggcgata taggccgcta ccgacttcat
      601 ttccatctcg gacatacgcg ccgagacgtt gcgcatcatg ttgccgggat cgttggcgcg
      661 cattccctgc ttgaaggcct gcagctgggc gacgatatac tcgggatgct ggccgaccag
      721 cgccggccac cccgccgggc cgttgccctt ggcggatggt gaatggcagc cgatacaggc
      781 cgcgacgctg gcgtcggtga tgccaccgcg gtagatgttt tcgcctttgt cgatcaattc
      841 cccgtcgaac gcgactccct tggcctgctg gctctggtag aaggcggcga gatttttcat
      901 atcgtcatcg ctcagtgtca cgaccatgcc ggtcatcgtc gcgttgctgc gccgcccttc
      961 cttgaaatct atcaattgct tgtagatata tgacgcgtgc tggccggcga gcgaaggcca
     1021 ggccggatta ctgctgttgc catccgcccc gtgacaggcg gcgcagatcg cgcttttggc
     1081 cttgccggcc tcgatatctg cggccgtcgc cgcaaatgac gacaggccgg acaaaaccag
     1141 caccatggct accagatggc tcgctttcat tatttctccc gggggaaaat ggtcggaaaa
     1201 tttgggcgca aatgtatatc ataccccagc cgcgcgttca acatcatccc tagaggctcg
     1261 atgcacattt ctttcagcaa ggcccgcttc ctgaccagcg ccttcgaact cgaccagttg
     1321 ccgcccgatc acggcgccga ggtcgcgttc gccgggcgct cgaacgccgg caagtcgagc
     1381 gcgatcaact gcctggttgg gcagaaaaac ctgtgcaaga ccagcaagac cccggggcgc
     1441 acgcaattga tcaatttttt cgaactcaca ccggacagcc gcctggtgga tctgccgggt
     1501 tacggtttcg cgaaggtgcc gaagaccttg cgcgcgcact gggaccgagt cctgtcgacg
     1561 ttcctgctcg agcgtgccgc cctgcgcggg ctggtgatcg tcgtcgatat tcgtcgcggc
     1621 ctcggcgaac tcgaccaggc attgatcgat atgatcggcg cccgcctgcc actgcacatc
     1681 ctgctgacca aggcggacaa gctgtcgcgc tcggccgcgc gcaaagcgat gatcgaaacc
     1741 gaacgaaggc tgcctggccc cgcgcacagc ctgtcgtcac tttccgtcct cagccgcgag
     1801 ggtatagatc ctctcgagca gcgttgccgg tcgtggctgg aggcggagga gctgggcgaa
     1861 tgacaaaaaa aagccctggc agaaaactgc cagggtaaaa cgtgtgatcc ggatggggtt
     1921 tccgaatctc ttgtccgctc ataggaggga gaacggacat tagcgctggg ctaaagcgct
     1981 aatcaggttg acatccagcc ggtcgaaaag ttcccttcat gactgccacc cctgtccttg
     2041 tgtgaccgcc atccttgacc cgactctctg tccaatgatc cactaactgg ggatgagttc
     2101 agtggaatca agccagcaca acaaacttag tctctatacg actattggcc catgctggat
     2161 ctctaaaata tcgaaaattt tgtcgcgatc cgaacgctag tcgacgggcg gcgccgtttt
     2221 cccggcaaac ggtcgccggc aggctgccaa agcggctttt cgagtcgatt gccggcgccg
     2281 agccgggatc gcggagcgaa gcgcgggggg catcttcgcg gcgatttttt tccgctgatc
     2341 ggttcgagcc ggttcgatca cccggccggg tcagccggcc gagtggcttt tgcttgtgca
     2401 acgaacttcg gcctgatttc gctttctggc gtcgccagcg ccgcaagggc aagggcaagg
     2461 gcaagggcaa gggcaaggcg aaatttctca taatgcggga gtttattccc cataaatgac
     2521 cgcgttgcgc gaaatttcaa cgccgccatc gtggcgcgca gcagccagaa agcgaaatta
     2581 gtgggcttcg ttccagtttt ggcccacccc cgcatccact tccagcgcca ccgataaatc
     2641 cgccgccccg gtcatgatct cggcaatccg ctcgcggcaa tcgtcgaccg tagcggcgtt
     2701 tacctcgaac accagttcgt cgtgaacctg catgatcatc tggcagtcat cgcgtgccga
     2761 cagccagcgg tgtacctcga tcatcgcccg cttgatgata tccgcggcgc tgccctgcat
     2821 cggcgcgttg atcgctgtgc gctcggcata ctggcgaaga ttaccgttgc gcgcgttgat
     2881 gtccggcagg tacaggcggc ggccgaaaac ggtttcgacg taaccctgct cgcgggcgcg
     2941 ctccttggtg tcgtccatgt agcgcctgac gccggggtag cgctcgaaat aggtgtcgac
     3001 gtactgctgc gcctcgttgc ggccgatgcc gagctgtcgt cccaggccga acgccgacat
     3061 accgtagatc aggccgaagt tgatcgcctt ggcggcgcgc cgctgctcgg cgctgacctc
     3121 gtcgaggctg gcgccgaaaa cctcggccgc g
//
LOCUS       DATCZV010000416         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_87379, whole genome shotgun sequence.
ACCESSION   DATCZV010000416 DATCZV010000000
VERSION     DATCZV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87379"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..302
                     /locus_tag="VIW27_09425"
     CDS             63..302
                     /locus_tag="VIW27_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein, HigA family"
                     /protein_id="HEY5739935.1"
                     /translation="MHRNCHHPGAYLQELLIWHQLSAEDLAREIDVPCRAIVDLTSAR
                     RGIDATISEGLAGFFGNSAHFWLDLQWRFDRRNDA"
     gene            667..2262
                     /locus_tag="VIW27_09430"
     CDS             667..2262
                     /locus_tag="VIW27_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5739936.1"
                     /translation="MNRIRITLLGIAALIMAGVSPSQAATPKNTFVMAKDIADIITMD
                     PAEVFELTTGEIIANIYDRIMMFEPEDLQNLVGGVAESWEVSADGKTITFTIRPGLKF
                     HSGNPVRAEDVAWSLQRVVKLNKTPAFIITQFGWNADNVDSLVKALDERRVAVTITED
                     FSPGLVLNALSAGVASVVDKQLVMSHEKDGDLGYDWLKSNSAGSGAFSLKTWKANEIV
                     TLEANPEYRHGAPGMKRVILRHVAEPSSQRLLLEKGDIDMARNLTPDQIKGIEGNAEI
                     KIDGYPKGTIVYMAANAGHPILGKPEVVQALRNLVDYESMEKSFLAGQFVVHQAFWPG
                     GLWAALEDNPYRQDVGKAKALLKQAGHADGFELTIHTLTNSPFPEIAQSVQANLAKGG
                     IKANIVTLEGKTLWPKYRAREHDIILARWSPDYVDPHSNADAFAHNPDNRLEAKLTGV
                     LAWRNAWADQASNELAVSARNELDLGKRKQQYIELQRMLQQKGPYVIMFQQNEQVARR
                     DNLRGFVSGSNFDLVFYRNVIKR"
BASE COUNT          492 a          770 c          759 g          420 t
ORIGIN      
        1 cgcccctcgc tggctggatt ttcgacaggc tgttcggctt cgcccgccat ggaggcaatt
       61 aaatgcatcg gaactgtcat catccggggg cttacctgca ggaactgctg atctggcacc
      121 agctgagcgc cgaggacctg gcccgcgaga tcgacgtccc gtgccgcgcg atcgtcgatt
      181 tgacctcggc tcggcgcggc atcgacgcga caatttccga ggggctcgcc ggcttcttcg
      241 gcaactcggc gcatttttgg ctcgatttgc agtggcgatt cgatcgccgc aacgacgcct
      301 gaagccagcc gcgggctcga ttgcggcagg ggtgccgcaa ccaatccccg gcatgccggc
      361 cgccggcgcg gcgctaaccg ccggcgacga taggtgttgc gagaattccc gccagcgaaa
      421 aacgccgcaa caacggcgtc gccgcaggcc gcgcaagcaa tcgccggcat agccgcggca
      481 cccgtggcgc cacggggcct ggccatcgcc cgtcggatgt agtttcgaga ccgtgcaaac
      541 tccccgctaa acgccgagaa gtcggtttat tgggtgcgaa ctgtggtaac attttcggcg
      601 cttcgcatta ccgcgaggtg tcgcaataac cataaatcaa cgaccctaac cgtcgaggag
      661 gcagttgtga acagaatcag aataaccctg ctgggaatcg ccgcgctgat catggcgggc
      721 gtatcgccgt cgcaggccgc gacgccgaaa aacaccttcg tcatggccaa ggatatcgcc
      781 gacatcatca ccatggaccc ggccgaggtg ttcgaactga ccaccggcga aatcatcgcc
      841 aacatctacg atcgcatcat gatgttcgag cccgaagacc tgcaaaacct ggtcggcggg
      901 gtcgccgaaa gctgggaagt cagtgccgat ggcaagacca tcacctttac cattcgcccc
      961 gggctgaagt tccattccgg caatccggtg cgcgccgagg acgtggcctg gtcgctgcag
     1021 cgcgtggtca agctcaacaa gaccccggcg ttcatcatca cccagttcgg ctggaatgct
     1081 gacaacgtcg acagcctggt caaggcgctc gacgagcgcc gcgtggcggt gaccatcacc
     1141 gaggattttt cgccgggcct ggtgttgaac gcgttgtccg ccggtgtcgc ctcggtggtc
     1201 gacaagcaac tggtgatgag ccatgaaaag gacggcgacc tgggctatga ctggctgaag
     1261 tcgaacagcg ccggctccgg cgccttcagc ctgaaaacct ggaaggcgaa cgagatcgtc
     1321 accctggaag ccaatcccga ataccgtcac ggcgcgccgg gtatgaagcg ggtgatcctg
     1381 cgccacgtcg ccgaaccgtc gtcgcaacgg ctgctgctgg aaaagggtga tatcgatatg
     1441 gcgcgtaacc tgacgccgga ccagatcaag ggtatcgagg gcaatgccga gatcaagatc
     1501 gacggctatc cgaagggaac catcgtctac atggcggcca atgccggcca cccgatactc
     1561 ggcaagcccg aggtggtgca ggcgctgcgc aacctggtcg actatgagag catggagaaa
     1621 tcgtttctcg ccgggcaatt cgtcgtccac caggcattct ggccgggcgg gctgtgggcc
     1681 gcactcgagg ataaccccta tcggcaggat gtcggcaagg cgaaggcgct actgaaacag
     1741 gctggtcatg ccgacggctt cgagctgacc attcatacgc tgaccaattc gccgttcccg
     1801 gagatcgcgc aatcggtgca ggccaatctc gccaagggcg gcatcaaggc gaatatcgtc
     1861 acgctggagg gcaagacgct gtggccgaag taccgcgccc gcgagcatga catcattctc
     1921 gcgcgctggt cgccggacta tgtcgacccg cattccaatg ccgatgcttt cgcccacaat
     1981 cccgacaatc ggctggaagc caagctgacc ggggtgctcg cctggcgtaa cgcctgggcc
     2041 gaccaggcca gtaacgagct cgcggtgagc gcgcgcaacg aactcgatct cggcaagcgc
     2101 aagcagcagt acatcgaact gcagcgcatg ctgcagcaga agggtccgta cgtgatcatg
     2161 ttccagcaaa acgagcaggt cgcgcgacgc gataatctgc gcggcttcgt ctccggttcg
     2221 aacttcgacc tggtgtttta ccgcaacgtg atcaagcgct gagctttacg cacagcggct
     2281 gaatgacatc cgccacgacc agcggcccgg cggcgggcca ctccccgccg tcggccctgg
     2341 ccagggtcgc cggagtgctg gcgagcctcg cgctgacatt catcgggctg accgcgatca
     2401 ccttcgtcat cggccgggtg atgccggtgg atccggtgct g
//
LOCUS       DATCZV010000417         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_299044, whole genome shotgun sequence.
ACCESSION   DATCZV010000417 DATCZV010000000
VERSION     DATCZV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299044"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VIW27_09435"
     CDS             <1..378
                     /locus_tag="VIW27_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739937.1"
                     /translation="YLGLLAQVWRLKSEARQQPLQFPPELLAEGRQIAERERSLYFAR
                     QSELQAQLAVFEQQVVQRKQELAELRSKQTFLAKAVALIHEELEITKPLLDKGQSGCA
                     RSATRPHTFTYCFSLLRGISPCT"
     gene            1007..1390
                     /locus_tag="VIW27_09440"
     CDS             1007..1390
                     /locus_tag="VIW27_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739938.1"
                     /translation="MEDADRYRCRYIYLEQTSDPALQAAAACEALTHIDGILLAAAHD
                     EHSVHIVYSLDELSFELVIDLLSELDFELDNSFLNSLRKTIYCYLEDNARDHMHIDEA
                     ETPPEDLESPEIPHQSKEQYWDDYH"
     gene            1391..2122
                     /locus_tag="VIW27_09445"
     CDS             1391..2122
                     /locus_tag="VIW27_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739939.1"
                     /translation="MYAITLFDIAIAFIPVLVTLVIIFFWSSNLRQASIAIVRMLVQL
                     LLIGYALEFIFNADSQWIILAVLCFMLLAASWIALGALPVRRATLLAYSLAAIAVGGI
                     FNLALITQGVLHADPWYQPSIMIPLAGMIFSNSMNSVSLAGERLYSELEHHQDYARAR
                     NTAFHAAMIPLTNSLLAVGLVSLPGMMTGQILAGTSPLIAARYQIMVMCMIFSSAGIS
                     AAMFLWLLRLQRARQQVNSGSDSCG"
     gene            2113..>2325
                     /locus_tag="VIW27_09450"
     CDS             2113..>2325
                     /locus_tag="VIW27_09450"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5739940.1"
                     /translation="MWIALSLLAGLLLVVLVYLLSFDGRFRVERSREIAAPTDRVFAA
                     VADLRTWPAWSPWLLYEPDAEIVYGDN"
BASE COUNT          435 a          730 c          667 g          493 t
ORIGIN      
        1 tatctcggtc tcttggccca ggtgtggcgc ctgaaatcgg aggcgcgcca gcagcccctc
       61 cagtttcccc cggaacttct ggccgagggg cggcagatcg ccgagcgcga gcgctcgctt
      121 tatttcgccc ggcagagcga actccaggcg caactggcgg tattcgagca gcaggtcgtc
      181 cagcgcaaac aggaactggc ggaactcaga agtaagcaaa ccttcctcgc caaggccgtc
      241 gcgctgatcc acgaggaact cgaaatcacc aagcccctgc tcgacaaggg ccagtccggg
      301 tgcgcccgga gcgcgacccg gccgcatact tttacgtatt gcttctcgct attgcgtgga
      361 atttcgcctt gcacttgact ccctcacgac agcagagacc tccctgagat caacttttcc
      421 ggtaatgccg tgattcgcgc aagacgcttg ggcacacgga tgtgcccatg ccgggcgcgt
      481 cgggaacgcg gcccggccga gtacttgagc atactgttcg cgccattgcg cgaaattccg
      541 ccttgctatc gcggtgcttg ggcacacgga tgtgcccatg ccggacgcgc cgggagcgaa
      601 gcccggccgc gacgcccaaa gccttcacgt cgatagaaat ttctctatct cctgaggttc
      661 caggcaaagc gcttgggtac atggatgtac ccagtccggg tgcgcccgga gcgcgacccg
      721 gccgcatact tttacgtatt gcttctcgct attgcgtgga atttcgcctt gcccttggct
      781 cactcacatc ggcatagact ttccggcgat ttaagatttt ccaattttga gaatagtcga
      841 attccgcctt gctctcgacg cagttgcgca catccagttg agcaggtcga gggcgccata
      901 aagcgcggcc cgagctgcga ctcgcgtcga tatgcagcat cgcgatcgcg gtgcgcggca
      961 gccggaaacg attgaaaatg cgcaggggtg ggcgtaggct ttatccatgg aagacgccga
     1021 tcgttatcga tgtcgctata tctacctgga gcagactagc gacccggcac ttcaggccgc
     1081 agccgcctgc gaggcgctca ctcatatcga cggcatcttg ctggccgcgg cccacgacga
     1141 gcatagcgtt cacatcgtct attcgctcga tgagctgtcg ttcgagctgg ttatcgattt
     1201 gctctccgag ctggatttcg agctggataa ttcatttctg aattcgctgc gcaagaccat
     1261 ctactgttat ctcgaggaca acgcgcggga tcacatgcac atcgacgaag ccgagacgcc
     1321 gcccgaagat ctcgaaagcc cagagatccc acaccagagc aaagagcaat actgggacga
     1381 ttaccattag atgtatgcaa taacgctgtt cgatatcgcg attgcattca tcccggtgct
     1441 ggtgacgctg gtgatcatct tcttctggtc cagcaacctc aggcaggcga gcatcgctat
     1501 cgtccgcatg ctcgtgcaac tactgctgat cggttatgcg ctggagttca tcttcaacgc
     1561 cgacagccag tggatcatcc tcgcggtact ctgtttcatg ctgctcgccg cgagctggat
     1621 cgcgctcggc gcgctgcccg tcaggcgcgc taccctgctg gcttacagcc tggcggcgat
     1681 cgccgtcggc ggtatattca acctggcgct gatcacccag ggcgtgctgc atgccgatcc
     1741 gtggtaccag ccgtcgatca tgattccgct ggccggaatg atcttttcca actcgatgaa
     1801 tagcgtcagc ctggccggcg agcgcctcta cagcgaactc gaacaccacc aggattacgc
     1861 gcgcgcgcgc aacaccgctt ttcatgcggc gatgattccg ctgaccaact cgctgctcgc
     1921 ggtcggcctg gtctcactgc ccggcatgat gaccggacag atactggccg gcacctcgcc
     1981 gctgatcgcg gcgcgctacc agatcatggt gatgtgcatg atcttcagct cggccggcat
     2041 ttcggcggcc atgttcctgt ggttgctgcg cctgcaacgc gcccgacaac aggtaaacag
     2101 cggtagcgac tcatgtggat agcgctgtcg ctgctcgccg gcctgctgtt ggtggtcctc
     2161 gtctatctgc tcagtttcga tggccgcttt cgcgtcgagc gcagccgcga aatcgccgct
     2221 ccgaccgaca gggtttttgc cgccgtcgcc gacctcagga cctggcccgc gtggagccca
     2281 tggttgctgt acgagcccga cgccgagatc gtatacggtg acaat
//
LOCUS       DATCZV010000418         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_246854, whole genome shotgun sequence.
ACCESSION   DATCZV010000418 DATCZV010000000
VERSION     DATCZV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246854"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VIW27_09455"
     CDS             <1..854
                     /locus_tag="VIW27_09455"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY5739941.1"
                     /translation="HFRLDLPPADLSDWDGVVEAMENPQNEISIGFVGKYVDHTDAYK
                     SLNEALDHAGIHTRTRVNIVKIDSDIIESDGTAMLENLDAILVPGGFGSRGIEGKIAA
                     ANYARIHDVPYLGICLGMQVAVIEYARNVLGLSKAHSSEFNPKTPDPVIALVTEWVDR
                     DGSVEVRDENVDLGGTMRLGAQTCKLVPGTRTRRAYGAEQIHERHRHRYEFNNNYLQR
                     LQDAGLVISGLSVDNELVEMVELSDHPWFIGCQFHPEFTSQPREGHPLFNSFISAAVE
                     YSKARRK"
     gene            851..1696
                     /gene="kdsA"
                     /locus_tag="VIW27_09460"
     CDS             851..1696
                     /gene="kdsA"
                     /locus_tag="VIW27_09460"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019593221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY5739942.1"
                     /translation="MNLCGFEVGIEHPLFLIAGPCVIESEQLALDTAGELQQICAELG
                     VNFIYKSSFDKANRSSSKSFRGPGLDRGLEILEKVGSSLALPVLTDVHEDTPLDEVAS
                     VVDVLQTPAFLCRQTNFIQNVARHNKPVNIKKGQFLSPWDMQQVVDKALETGNRQIMV
                     CERGVSFGYRNLVSDMRSLAVMRETGCPVVFDATHSVQLPGGQGSSSGGQREFVPVLA
                     RAAVAAGVAGLFMETHPQPEEALSDGPNAWPLGRMRELLQVLVEIDRVTKASPFPEQE
                     WLGDA"
     gene            1710..>2038
                     /gene="eno"
                     /locus_tag="VIW27_09465"
     CDS             1710..>2038
                     /gene="eno"
                     /locus_tag="VIW27_09465"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960226.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY5739943.1"
                     /translation="MSKITKLVGREILDSRGNPTVEAEVHLEHGVVGRAAAPSGASTG
                     EREAIELRDGDTGRYLGKGVTRAVGHIESEIADVVLGRDCNDQAGLDRAMIELDGTEN
                     KSRLGANA"
BASE COUNT          406 a          622 c          643 g          367 t
ORIGIN      
        1 agcatttcag gctggatttg ccgcccgccg acctgtcgga ctgggatggt gtcgtcgagg
       61 cgatggaaaa cccgcaaaac gaaatttcga tcggcttcgt cggcaagtat gtcgatcaca
      121 ccgacgccta caagtcgttg aacgaagccc tcgatcatgc cggtatccat acccgcaccc
      181 gggtcaatat cgtcaagatc gattccgata tcatcgaaag cgacggcacg gcgatgctcg
      241 aaaatctcga cgcgatcctg gtaccgggcg gtttcggcag ccgcggcatc gagggcaaga
      301 tcgccgccgc caactatgcg cgcatccacg atgtgcctta tctcggtatt tgcctcggta
      361 tgcaggtcgc ggtgatcgag tacgcgcgca acgtgctggg cctgagcaaa gcgcacagtt
      421 cggaattcaa cccgaagacg ccggacccgg tgatcgcgct ggttaccgag tgggtcgacc
      481 gtgacggttc ggtcgaggtt cgtgacgaga acgtcgatct cggcggcacc atgcgcctcg
      541 gcgcgcagac ctgcaagctg gtgcccggca cccgcacgcg gcgcgcctat ggcgccgagc
      601 aaatccacga gcgccatcgc catcgctacg aattcaacaa caactacctg cagcggttgc
      661 aggacgccgg gctggtcatc tccgggttgt cggtcgacaa cgaactggta gagatggtcg
      721 aactgtcgga tcacccgtgg ttcatcggct gccagtttca tcccgaattc acttcgcagc
      781 cgcgcgaagg gcacccgctg ttcaacagtt ttatcagcgc ggcggtcgag tacagcaagg
      841 ccaggcgcaa atgaatctct gcgggttcga ggtcggtatc gagcatccgc tgttcctgat
      901 cgccggcccc tgcgttatcg aatcggaaca actggcgctc gacaccgccg gcgagctgca
      961 gcagatctgc gccgagttgg gcgtcaattt catttacaag tcgtcattcg acaaggccaa
     1021 ccgctcgagc agcaagagtt ttcgcggtcc cggcctcgac cgcgggctcg agatcctgga
     1081 gaaggttggc agcagcctcg cgctgccggt gctgaccgac gtccacgaag acacgccgct
     1141 cgacgaggtc gccagcgtcg tcgacgtgct gcaaaccccg gcctttctgt gccgacagac
     1201 caattttatt cagaatgtcg cccggcataa caagccggtc aacatcaaga aagggcaatt
     1261 cctgtcgcca tgggacatgc agcaggtggt cgacaaggcg ctggaaaccg gtaaccggca
     1321 gatcatggtc tgcgagcgcg gcgtgagttt cggctatcgc aacctggttt ccgacatgcg
     1381 ttcgctggcg gtgatgcgcg aaaccggctg cccggtagtg ttcgacgcca cccactcggt
     1441 acaattgccc ggcggccagg gttcgtcatc cggcgggcaa cgcgagttcg tgccggtgtt
     1501 ggcgcgtgcc gcggtcgcgg cgggtgtcgc cgggttgttc atggaaaccc atccgcaacc
     1561 ggaggaagcc ctcagcgacg ggcccaacgc ctggccgctg gggcggatgc gcgaactgtt
     1621 gcaggtgctc gtcgaaatcg atcgcgtaac caaggcttcg ccgttccccg aacaggaatg
     1681 gctcggcgac gcctgatagc ggagtcacta tgagcaaaat cactaaactg gtcggtcgcg
     1741 aaatactcga ttcacggggc aatccgaccg tcgaagcgga agtccatctc gagcatggcg
     1801 tggttgggcg agccgccgcg ccgtcgggtg cgtccaccgg cgagcgcgag gcaatcgaat
     1861 tgcgcgacgg cgataccggg cgctacctgg gcaagggcgt gacccgcgcg gtcggccata
     1921 tcgagagcga aattgccgat gtcgtgctcg gccgtgactg taacgatcag gccggcctcg
     1981 atcgggcgat gatcgaactc gatggtaccg aaaacaagag ccggctcggc gccaatgc
//
LOCUS       DATCZV010000419         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_617404, whole genome shotgun sequence.
ACCESSION   DATCZV010000419 DATCZV010000000
VERSION     DATCZV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617404"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VIW27_09470"
     CDS             <1..253
                     /locus_tag="VIW27_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010441429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5739944.1"
                     /translation="VITIAGLQLGAIIAFALITETVFQWPGVGLMFVNAVFFVDIPVM
                     SAYLLLVGTVFVVINLIVDLLYFAIDPRLRDGQLNRRAT"
     gene            250..1176
                     /locus_tag="VIW27_09475"
     CDS             250..1176
                     /locus_tag="VIW27_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5739945.1"
                     /translation="MSVERSTWQRFIESDFFFDFKRSPVTIVSFTIVVTLILLALLAD
                     FVAPTNPFDPASLNLMNGFTPPLEPNAFTGETFVLGTDDQGRDLLSAIIYGLRVSLFV
                     GLTAVILAMLIGVSLGLLAGYRGGWIESLIMRVADIQMTFPSILIAVLIFGVARGLLP
                     PDLRDELAISVLIIAIGLSEWVPFARTVRGTTMVEKEKEYVQAARLIGLGKVQIMVRH
                     ILPNVLSPVLVIATITLALAIIAEATLSFLGVGAPPTEPSLGTLIRIGQDFLFSGEWW
                     ILLFPSVTLLALALSVNLLGDWLRDTLNPRLK"
     gene            1173..2132
                     /locus_tag="VIW27_09480"
     CDS             1173..2132
                     /locus_tag="VIW27_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5739946.1"
                     /translation="MSQSILRVRNLRVVFPHRHGELVAIDDISFDVRKGEILGFVGES
                     GAGKSMTGAAIIGLIDPPGYIERGEIWLDDERIDQLDSGAMTRIRGDRISMVFQDPLT
                     SLNPLRTIGDQLVETIQTHLPVGDDEAKRKAMAGLEEVGIDPSRFDAYPHEFSGGMRQ
                     RVVIALALSAEPELIIADEPTTALDVSVQAQVLKLLRRLCQEHGASVILITHDMGVIA
                     ETTDRVAVLYAGRLAEIGPTAEVLNEPQHPYSRGLIASTPSVEQGDIDTRLFQISGAM
                     PRLDELPAGCAFNPRCEHRMERCVDQRPDLLQDRVSCWLYADG"
BASE COUNT          358 a          760 c          692 g          443 t
ORIGIN      
        1 ggtgattacg atcgccggtt tgcagctcgg cgcaatcatc gcgttcgcgc tgattaccga
       61 gaccgtgttt cagtggcccg gtgtcggcct gatgttcgtc aacgcggtct tcttcgtcga
      121 catcccggtg atgtcggcct acctgctgct ggtcggcacc gtattcgtcg tcatcaacct
      181 catcgtcgat ctgctctatt tcgcgatcga cccgcggctg cgtgacggcc agctgaatcg
      241 gcgggcgaca tgagcgtcga acgcagcacc tggcaacgct tcatcgagag cgatttcttc
      301 ttcgatttca agcgctctcc ggtcaccatc gtgtctttca cgatcgtcgt cacgctgatc
      361 ctgctcgctt tgctggccga tttcgtcgcg cccaccaacc ccttcgaccc ggcctcgctg
      421 aacctgatga acggcttcac gccgccgctc gaaccaaacg cttttactgg cgaaaccttc
      481 gtcctcggca ccgacgacca gggccgcgac ctgctgtcgg cgatcattta cggtttgcgc
      541 gtatcgctgt tcgtcggcct caccgcggtg atcctggcga tgctgatcgg ggtcagtctc
      601 ggcctgctcg ccggctaccg cggcggctgg atcgagtcgt tgatcatgcg cgtcgccgac
      661 attcagatga ccttcccgtc gatcctgatc gcggtgctga tcttcggcgt cgcgcgcggc
      721 ctgctgccgc ccgatttgcg cgacgagctc gcgatcagcg tgctgatcat cgccatcggc
      781 ctgtccgaat gggtgccgtt cgcgcgcacc gtgcgcggca cgaccatggt cgagaaggaa
      841 aaggaatacg tccaggccgc gcgcctgatc ggtctcggta aagtgcagat catggtgcgc
      901 cacatactgc cgaacgtgct gtcgccggtg ctggtcatcg cgaccattac gctcgcgctc
      961 gcgatcatcg ccgaggcgac gctgtcgttc ctcggcgtcg gcgcgccgcc gaccgagccg
     1021 agcctcggta cgctgatccg catcggccag gatttcctgt tttccggcga gtggtggatc
     1081 ctgctgttcc cgtcggtaac gctgctcgcg ctggcgctgt cggtcaacct gctcggcgac
     1141 tggctgcgcg acacgctgaa cccgaggctc aaatgagtca gtctatcctg cgcgtgcgca
     1201 acctgcgcgt ggtgtttccg caccgccacg gcgagctggt ggcgatcgac gatatctcgt
     1261 tcgacgtacg caagggcgaa attctcggct tcgtcggcga atcgggcgcc ggcaagtcga
     1321 tgaccggcgc cgcgatcatc ggcctgatcg acccgccggg ttacatcgag cgcggcgaaa
     1381 tctggcttga cgacgagcgc atcgaccagc tcgactccgg cgccatgacc cggattcgcg
     1441 gcgatcgcat cagcatggtg ttccaggatc cgctgaccag tctcaacccg ctgcgcacga
     1501 tcggcgatca gctggtcgaa accatccaga cgcatttgcc ggtcggcgac gacgaagcga
     1561 aacgcaaagc gatggcaggg ctcgaggaag tcggcatcga cccgtcgcgt ttcgacgcct
     1621 acccgcatga attctcgggc ggtatgcgcc aacgcgtcgt catcgcgctg gcgctgtcgg
     1681 ccgagccgga actgatcatc gccgacgagc cgaccaccgc gctcgacgtg tcggtgcagg
     1741 cgcaggtgct caaactgctg cgcaggctgt gccaggagca cggcgcgtcg gtcatcctga
     1801 tcacgcacga catgggcgtc atcgccgaaa ccaccgaccg tgtggcggtg ctctacgccg
     1861 ggcggctggc cgaaatcggc cccaccgccg aggtgctgaa cgagccccag cacccttaca
     1921 gccgcggcct gatcgcgtcg acgccgtcgg tcgagcaggg cgatatcgac acccgcctgt
     1981 tccagatttc gggcgcaatg ccgcgcctcg acgagctgcc ggccggctgc gcgttcaacc
     2041 cgcgctgcga gcaccgcatg gagcgctgcg tcgaccagcg cccggacctg ctgcaggacc
     2101 gtgtctcctg ctggttgtac gccgatggat gagttcatcc gcgtcgagca actgcacaaa
     2161 cgctacgacc tgtcggagcc atggcttgcg cgcatgatct accggcggcc aaagcgtttc
     2221 ctcgacgctg tcgacgacgt ttccttcagt atc
//
LOCUS       DATCZV010000420         8255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_299173, whole genome shotgun sequence.
ACCESSION   DATCZV010000420 DATCZV010000000
VERSION     DATCZV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299173"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..853
                     /locus_tag="VIW27_09485"
     CDS             464..853
                     /locus_tag="VIW27_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739947.1"
                     /translation="MQAKLLDRQWLKRVIEVVDGDKVTTLVYSGRGIGSEKVIVDGAE
                     TSDRSRLLWFVPRFEVASGEDSYVFQVRVWPWLTLRSLKIEKNGVPIYTEGSEPYRVS
                     GFTEVAQLASFAALLIGPAFLVLLALR"
     gene            1087..1536
                     /locus_tag="VIW27_09490"
     CDS             1087..1536
                     /locus_tag="VIW27_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739948.1"
                     /translation="MPNDALLDGLITLGCGVNTVDGLTLVTLPDDYDEFVFERLGEWL
                     YLGTTLMTPEDFGDSNYTAALDRFLLELQHRSLGCHFSYDAAGFLTIGTELPLNQQNP
                     DEVLQTMEQICFVIDACLPLCDRVLDSGELPVDKEIDSAFGVSENLH"
     gene            1722..2396
                     /locus_tag="VIW27_09495"
     CDS             1722..2396
                     /locus_tag="VIW27_09495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5739949.1"
                     /translation="MPGFEGNGKLFAPLIPWLEPHFKLSIIKYPDLNSFEDYVDCAQN
                     QLPAEPGYSLLAESFSGPVAIALLAQRPGQIGPSILSSTFARSPLAALTKMANHVPQQ
                     MFSIGTLSEYCLDVNAVYDEDQSETQPLPLNIAEQLDGVVLRHRIAVMSRIDVSALLP
                     QIEAPILLLHGQRDRIVSELEARLITQNLPGARRVDFDAPHLLLQIQPHQCAEHIIRH
                     IRPDVL"
     gene            2690..3106
                     /locus_tag="VIW27_09500"
     CDS             2690..3106
                     /locus_tag="VIW27_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY5739950.1"
                     /translation="MICPRCNTAMEVRDHDGIRSSICPQCNGIWIGGAALARLFERDR
                     DAPHIEEALEAILGVDYRSSRRQCPACRGRHLKAVLVDDVELDYCVACKGLYFDQGEL
                     EQVLPGTFGEPGSTARSPRGSTSRFWAVITRFIGGD"
     gene            3121..3393
                     /locus_tag="VIW27_09505"
     CDS             3121..3393
                     /locus_tag="VIW27_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739951.1"
                     /translation="MARLSMFLALLAAVSVTLAIGMAVSVSRQGGVSGLWFLSSVAVM
                     LALLSSALSLMLASILIARHDPARPWRAVWVSLAAVAAAAGYLLLL"
     gene            3634..4188
                     /locus_tag="VIW27_09510"
     CDS             3634..4188
                     /locus_tag="VIW27_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013796779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5739952.1"
                     /translation="MAVSPPATFSLSLSHFELYVRDIAAMEDFYTRYLGFEVTDRGHG
                     AAAMVFLSRNPRAHHQLVLNPLEPAAATQSPVDHIAFRVGTLTALKSLHAVLTGSPAE
                     LQTVSHGNSWSLYFRDPEGNRLELFTDTPWHVDQPCRFEIDLGLDEPALREFTENAIR
                     DLPGFGDAGDWRQRRRAMRRGERA"
     gene            4185..4559
                     /locus_tag="VIW27_09515"
     CDS             4185..4559
                     /locus_tag="VIW27_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739953.1"
                     /translation="MSHQELAGSPLRGAIGGMLACWLLAAGTLPGPAWASAGWTDLVR
                     VAELVPTSKHYYEVRLPVGKNPSGCREQQWFYQNYDSPGAEQMFEVLLESLTADIRLR
                     VYVTGVCNLSGYAEFSSISVTR"
     gene            4567..5037
                     /locus_tag="VIW27_09520"
     CDS             4567..5037
                     /locus_tag="VIW27_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5739954.1"
                     /translation="MKTEATALVYRDAAEADLPVLIAMLAEDALGASREDPSSPLNPR
                     YLEAYRHIAADPNNALMVVEENGAAVGMLQLTFIPYLTHTGSWRCLIEGVRIHRDHRG
                     RGLGSEFMRWAIERAREHGCSIVQLTSDKQRPDALRFYEALGFSASHEGFKLRL"
     gene            5132..5962
                     /locus_tag="VIW27_09525"
     CDS             5132..5962
                     /locus_tag="VIW27_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5739955.1"
                     /translation="MSHETITDTDDFTIFEHQGWERACAGYEAHFGRLTRQSATALLD
                     AAQVTGGQRVLDVCCGPGMVTAVAAARGADALGLDFSAATVELATRLVPQATFHHGDA
                     QALPFDDAQFDAVVCGFGIIHLAQPRTALAEIYRVLKPGGRVAISVWQAPGPGNGFGL
                     LYGTITAHADMNVPLPEGPDFFQFSGPGALDRALAEHGFREPSTIGVDQVWEFDRETG
                     LVDAFLEGAVRARGLIMAQSEAVRDAIVAGVADGMQVFRAADGIYRLPMPALVGSGSK
                     "
     gene            6055..6375
                     /locus_tag="VIW27_09530"
     CDS             6055..6375
                     /locus_tag="VIW27_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY5739956.1"
                     /translation="MIHSTTNSIDGREVVQYHGVVTGEAILGANIFKDFFASIRDIVG
                     GRAAAYEQELGKARQIAFDEMTQQAAELGADAVLGIDIDYETVGAQGGMLMVSVSGTA
                     VSLR"
     gene            6517..7443
                     /locus_tag="VIW27_09535"
     CDS             6517..7443
                     /locus_tag="VIW27_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5739957.1"
                     /translation="MKILRLIPALASLCLASAPLQAGNGLELQQVTDEVYAIVGDLGN
                     RSADNLGNNATFGFVVTTAGVVLIDSGGTTRGARAIDAIIDTVTDAPVVAVINTGGQD
                     HRWLGNAYFRNGGARLIASRAAVDDQRNRLQDQFFMLGNLVGEELVRGTEAAYADETF
                     DDALDLTIGGTRFEIRYPGPAHTPGDSFVWLPAQQVMFSGDIVYVERMASVGAQSSSR
                     GWIEAFDAMAAYRPRYLVPGHGRATTLEHARRDTRDYLAQLRAAVGEFMDDGGDLSEI
                     GSVDQSAFTYLNNFEMLAGRNAQRVFEELEWE"
     gene            7531..7875
                     /locus_tag="VIW27_09540"
     CDS             7531..7875
                     /locus_tag="VIW27_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739958.1"
                     /translation="MPSALTRQLVIALLAGLACSAAIAQQAPSESVSFRCGLALDAVI
                     GELDTQTDNPVTQQALERLGEDQGEFVCVRTDDRTIVVYLLARDRTALEGKLVFTVDA
                     RTYEVLKTYYGP"
     gene            7882..>8255
                     /locus_tag="VIW27_09545"
     CDS             7882..>8255
                     /locus_tag="VIW27_09545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEY5739959.1"
                     /translation="MAELVYRAYRDEDEGGVIELWRRCGLIRPWNHPQADIRRKCADS
                     RQLFFVGERDGTPVASCMAGYDGHRGWIYYLAVAPELRRQGIAARLVSIAEAELRRLG
                     CPKIDLMIRDTNQQVIAFYRALG"
BASE COUNT         1483 a         2679 c         2581 g         1512 t
ORIGIN      
        1 cgacaagacg atctacaaac ggctggtgtc gagaccgcga ctgcaaaatg gccgcgagcg
       61 cctgctattg tccgccgatt cgagccaccc ggtttcggtg gttgcactgt tcggcatggg
      121 tggcgaatct gtcaccacga ccgcggattc cgcccgaaca cgccgtcgac aaagaccgct
      181 ccgggccgcg gaaacggtcg cggtgcttgt attttcacca attttggcta tacgaggcta
      241 tgcaaacgag tcgcaattac cgccgcttcg gggcatgccc gtaagagtct ttcggggcgg
      301 tgacttcccg caaggtacac agatacaggg aatgcaactg gaggattcga gaacacgata
      361 ccgggcaggc cttagcggcc gatgaccagc tgcacggtta tctcattgcc cccgccccgg
      421 ccacgcccac gagttttcag caacgcgcac agcggagaaa ttcatgcaag caaaactgtt
      481 agacaggcag tggttgaaaa gggttatcga agtagtcgac ggcgacaagg tgaccacgct
      541 cgtctactcg ggaaggggca tcggctcgga gaaggtcatc gtcgacggtg ccgagaccag
      601 cgatcgctcc cggttgctgt ggttcgtgcc gcgcttcgaa gtcgccagcg gcgaggactc
      661 ttatgtcttc caggtcaggg tctggccctg gctcacgctg cggtcgctga agatcgagaa
      721 aaacggggta ccgatctata ccgagggcag cgagccgtat cgcgtatcgg ggttcaccga
      781 agtcgcgcaa ctggccagtt ttgcggcgtt gctgatcggg ccggcatttc tggtcctgct
      841 ggcgctgcga tagccgccac gattctccgc ctgtctgctg tcgccagcag caccacgatt
      901 tcggttgccg tcggtcgccg ccctaataca atcttgctgc cgaaaccctg ttcccgaggc
      961 acgattcggg agtagactag gcgttgcaga taaacactga cgatgaaacc gaacttgcca
     1021 cttgttcgcc ggccgccgat atgcggtgac gcccccaacc cgatgcggat gaactagccg
     1081 atgccgatgc caaacgacgc cctgctggac ggattgatca ccctcggttg cggggtcaat
     1141 accgtcgatg gtttgactct ggttacactt cccgacgatt acgacgagtt tgtcttcgag
     1201 cgcctcggcg aatggctgta tctcggcacc acactgatga cacccgagga tttcggtgac
     1261 tcgaattaca ccgcggcact cgaccgtttc ctgctcgagc tgcagcatcg cagccttggc
     1321 tgtcactttt cctatgacgc agccggtttt ctgaccatcg gcaccgaact ccccctgaac
     1381 cagcagaatc ccgacgaggt gctgcagacg atggaacaga tctgtttcgt catcgacgcc
     1441 tgcctgccgc tctgtgaccg cgtgctggac agcggtgaat tgcccgtgga caaggaaatc
     1501 gacagcgcct tcggggtcag cgaaaacctc cactgaaccg gctgcagcac cacggagcag
     1561 ccgattccgg tggtgcagcg ttgtgcggcg ttgcggcgga atcacaattg tcgctgaatc
     1621 cctgccaatc gtgactataa taaaaatcat cgcaatccag cccgcgtggc caataaaaat
     1681 ccggagctat cccgttgagc cagctaccaa cactcgtcct gatgcctggt ttcgaaggta
     1741 acggcaagct gttcgcgccg ctcatcccct ggctggagcc gcatttcaag ctgtcgatca
     1801 tcaaatatcc ggacctcaac agcttcgagg attacgtcga ctgtgcacaa aaccagctgc
     1861 cggcagagcc cggctattcg ctgctggccg aatccttttc cggcccggtg gcaatcgcgt
     1921 tgctggccca gcgtcccggc cagatcggcc cctcgatact gagttcaacc ttcgcccgct
     1981 caccgctggc ggcgctcacc aagatggcca accacgttcc gcaacaaatg ttttccatcg
     2041 gcacgctgag cgaatactgc ctggacgtaa acgccgtcta cgacgaggat cagtccgaaa
     2101 cccagccgct gccgctcaat atcgccgaac aactcgacgg cgtcgtgctc agacaccgta
     2161 tcgcggtgat gagccgcatc gacgtgtcgg cgctgttacc gcaaatcgag gcgccgatat
     2221 tgctgctgca cggtcagcgc gaccgcatcg tctccgagct ggaagcgcga ctgatcaccc
     2281 aaaacctgcc tggcgccagg cgcgtcgatt tcgacgcgcc gcacctgttg ctgcagattc
     2341 agccgcacca atgcgctgag catatcattc ggcatattcg tccagacgtc ctgtaggcga
     2401 ccggcgccga tgctcgaacg ctggcgattc gcaggccgct caatctaccc gcggcaagcg
     2461 ctcgcaaagc cgctgatcga gccttgcttt accgctgacg aaagtcgccg ctgccggcgc
     2521 cccgcgcacg acaattagcg ccgcagcgac gaccaatgcc ggttctccga aaccaccgaa
     2581 aaccttataa tctcgctgtc gacgaactct cgccatcgcc tacggtttcg ccactcatgt
     2641 tttcgatcag gatttttgac cgccgcgcac atcggcgcat ccgccggcga tgatttgccc
     2701 gcgctgtaac acggccatgg aggtccgcga ccacgacggc attcgcagca gcatttgccc
     2761 gcagtgcaac ggcatctgga tcggcggcgc cgcgctcgcc cgcctgttcg agcgcgaccg
     2821 cgatgcgccg catatcgagg aggcgctgga ggcgatcctc ggtgtcgact atcgcagcag
     2881 ccgccgccag tgccccgcct gccgcggccg gcatctgaag gcagttctgg tcgacgacgt
     2941 cgaactcgac tattgcgtag cctgcaaggg tttgtacttc gaccagggcg aactggagca
     3001 ggtgttgccg gggacgttcg gcgaacccgg ctcgaccgct cgatcacccc gcggctcgac
     3061 gagtcggttc tgggcggtga tcaccaggtt catcggcggc gactaggcgg gctcaggccg
     3121 atggcccggt tatccatgtt tctcgccctg ttggccgccg tgtcggtaac tctcgccatc
     3181 ggcatggcgg tgtcggtatc gcgccaggga ggggtttccg gtctctggtt cctgagctcg
     3241 gtggcggtga tgctggcatt gctgtcgagc gcgctgtcgc tgatgctggc atcgatcctg
     3301 atcgcgcgtc acgacccggc gcggccgtgg cgagcggtct gggtatcgtt ggcagcggtg
     3361 gcggcagccg ccggctatct gctgctgctc tagggccgca agcgtcgtac ggcgagggtc
     3421 gcgtgaatac ggcagtttga cctgaagtct ttcggcgtca gtgcgtggca atgcagcagt
     3481 gctgatcgac gcgcgccggg cggcgacgcc ggtgcgtgat cgagccgtgg cgacggccgc
     3541 ggctcggcgc aagagcttgc catcgggagc agatttgccg atcatgctgt gtatcgctat
     3601 cagccgaatg ccagccaccg accaggattc cgcatggccg tctcgccacc agccactttt
     3661 tcgctgtcgt tgagccattt cgagctttac gtgcgcgaca tcgcggcgat ggaggatttt
     3721 tacacgcgtt acctcggctt cgaggtcacc gatcgcggcc acggcgccgc ggcgatggtc
     3781 ttcctgagcc gcaatccgcg cgcgcatcac cagctggtac tcaatccgct cgagcccgcg
     3841 gcggccacgc aaagtcctgt cgatcatatt gcgtttcgcg tcgggacgct gacggcgctg
     3901 aaatcgctgc atgccgtgct caccggcagc cccgccgaac tgcagacggt gtcacatggc
     3961 aacagctggt cactctattt ccgcgatccg gaaggcaacc gcctcgagct gtttaccgac
     4021 acgccctggc atgtcgacca gccgtgccgc ttcgagatcg atctcggcct cgacgagccg
     4081 gcgttgcgcg aattcaccga gaacgcgatt cgcgatctgc cgggattcgg cgacgccggt
     4141 gactggcggc aacggcggcg ggcgatgcgc cgcggggagc gcgcatgagc caccaggagc
     4201 tagccggatc tccgcttcgt ggcgccatcg gcggcatgct ggcatgctgg ctgctcgccg
     4261 ccggcacgct gccgggcccg gcatgggcga gcgccggctg gaccgatttg gtgcgcgtcg
     4321 ccgagctagt accgacgtcg aaacattact acgaggtgcg cctgccggtc ggcaaaaacc
     4381 cgagcggctg ccgcgaacaa cagtggtttt accagaacta cgactcgccc ggcgccgagc
     4441 aaatgttcga ggttttgctg gaaagcctga ccgcggacat tcgcctgcgc gtctacgtga
     4501 ccggggtctg caatctcagc ggctatgccg agttttcgtc gatcagcgtg acgcgctagg
     4561 cggcagatga agaccgaggc taccgcgctg gtctatcgtg acgccgccga ggcggacttg
     4621 ccggtactga tcgcgatgct cgccgaggat gcgctcggcg cgagccgcga ggatccgtcg
     4681 tcgccgctga atccccgcta cctggaggct taccgacaca tcgccgccga tcccaataac
     4741 gcgctcatgg tcgtcgagga aaacggggcg gcggtcggca tgctgcaact gacctttatt
     4801 ccctacctga cgcacaccgg ctcctggcgc tgcctgatcg agggggtcag gattcaccgc
     4861 gaccatcgcg gccgcgggct cggcagcgaa ttcatgcgct gggcgatcga gcgtgcgcgc
     4921 gagcacggct gcagcatcgt gcagctgact tccgacaagc agcgtccgga cgcgttgcgc
     4981 ttttacgaag cgctcggctt cagcgccagt cacgagggct tcaagctgcg cttatagcgc
     5041 gcagtagcca ctattccggg ctgtccaccc tcgccgggct gtcatacaat cgcttcgatc
     5101 gcgacgcgcc cattccgccg gaggctttga tatgagccac gagaccatca ccgataccga
     5161 tgactttacc atcttcgagc accaaggctg ggagcgcgcc tgcgccggct acgaggcgca
     5221 tttcggccgc ctgacccggc aatcggcgac cgcattgctc gatgcagccc aggtcaccgg
     5281 cggccagcga gtgctcgacg tctgctgcgg tccgggcatg gtgaccgcgg tggccgcggc
     5341 gcgcggcgcc gatgctctcg gcctggattt ctcggcggcc accgtcgaac ttgccacccg
     5401 gctggttccg caggccactt ttcaccacgg cgacgcgcag gcgctgccgt tcgacgacgc
     5461 gcagttcgat gccgtggtct gcggcttcgg catcattcac ctggcgcaac cgcgaaccgc
     5521 actggccgag atttatcggg tgctgaagcc cggcgggcgg gtggcgatca gcgtctggca
     5581 agctcccggg cccggcaacg gcttcggcct gctctacggc acgataacgg cccatgccga
     5641 tatgaacgtg ccgctgcctg aaggccccga tttcttccag ttcagcggcc ctggcgcgct
     5701 cgaccgggca ctcgccgaac acggttttcg cgaaccgtcg acgatcggcg tcgaccaggt
     5761 ctgggagttc gaccgcgaaa ccggcctggt cgatgccttc ctcgagggcg cggtgcgtgc
     5821 gcgcggcctg atcatggcgc agagcgaggc ggtgcgcgac gcgatcgtcg ccggcgtcgc
     5881 cgatggaatg caggtatttc gcgccgccga cggcatatat cgcctgccga tgccggcgct
     5941 ggtcggttcc ggctccaaat agtccggcgg ttcatcgggg ccgcgagcgc acgaatcggc
     6001 gctataatcg aatccgcgca aaaccgcgcg cgggcaacag cgaggaaaca tcgcatgatt
     6061 cattcgacga ccaacagcat cgacggccgc gaagtcgtgc agtaccacgg cgtggtgacc
     6121 ggcgaagcga tcctcggcgc gaatatcttc aaggattttt tcgccagcat tcgcgacatc
     6181 gtcggtgggc gcgccgctgc ctacgaacag gaactcggca aggcgcggca aatcgccttc
     6241 gatgaaatga cgcagcaagc cgccgagctg ggcgcggacg cggtcctcgg catcgacatc
     6301 gattacgaaa ccgtcggcgc ccagggcggt atgctgatgg tcagcgtcag cggtaccgcg
     6361 gtcagcctgc gctagtccgc gaacttgtca gcgtcccggc tttgaattag cctgcacgct
     6421 cgcgccgccc ggcgagccac ggcgtgctat ttctttcacc gcgcgaaatt aggatcggcg
     6481 acgccgcgcg ctaaccaccg acagcaacga gaaaccatga agatactccg actgattccg
     6541 gcactggcga gcctgtgcct ggcgagtgcg ccgctgcagg caggcaacgg actcgagtta
     6601 cagcaggtta ccgacgaagt ctatgcgatc gtcggcgatc tcggcaatcg cagcgccgac
     6661 aacctcggca acaacgcgac cttcggcttc gtagtcacca ccgccggcgt ggtgctgatc
     6721 gattccggcg gcaccacgcg cggtgcgcgc gccatcgatg caattatcga cacggtcacc
     6781 gatgcgccgg tagtcgcggt catcaatacc ggcggccagg atcaccgctg gctcggcaac
     6841 gcctatttca ggaacggcgg cgcacgcctg atcgccagcc gcgcggcggt cgacgaccag
     6901 cgcaaccgcc tgcaggacca gttctttatg ctcggcaacc tggtcggcga agaactggtg
     6961 cgcggtaccg aggccgccta tgccgacgaa accttcgacg acgcactcga tctgacgatc
     7021 ggcggcacgc gtttcgagat ccgctatccc ggcccggcgc acacgcccgg tgacagtttc
     7081 gtctggctgc cggcacagca ggtcatgttc agcggcgaca tcgtctacgt cgagcgcatg
     7141 gcgtcggtcg gcgcacaatc gagcagccgc ggctggatcg aggcattcga tgcgatggct
     7201 gcctaccggc cgcgctacct ggtaccgggc catggccggg caacgacgct cgagcacgcg
     7261 cgccgcgata cgcgcgacta cctggcccag ctgcgcgccg cggttggcga gttcatggac
     7321 gacggcggcg acctgagcga aatcggcagc gtagatcagt ccgcctttac ttacctgaat
     7381 aatttcgaaa tgctcgccgg gcgcaacgcg cagcgggtat tcgaggaact cgagtgggaa
     7441 tagccggcgc aggccgccga gcggctgcgc aaagcgccgc gatgatgcga taatagctgc
     7501 tttcgcttgc cactcgagat caccaaaagc atgccttcgg cactcacgcg tcaactcgtc
     7561 atcgctttgc ttgccggcct cgcctgcagt gccgcgatcg cgcagcaggc gccgtcggag
     7621 tcggtttcgt ttcgctgcgg cctggcgctc gacgcggtga tcggggagct cgacacgcag
     7681 accgataacc cggtcaccca gcaagctctc gagcgcctgg gcgaagatca gggcgagttc
     7741 gtctgcgtca ggaccgacga caggacgatc gtcgtctacc tgttggcgcg cgaccgcacc
     7801 gcactcgaag gcaagctggt gttcacggtc gatgcgcgta cctacgaagt tttgaaaacc
     7861 tactacgggc cgtgaacggc gatggctgaa ctggtctatc gcgcttaccg cgacgaggac
     7921 gagggcggcg tgatcgagct gtggcggcgc tgcggcctga ttcggccgtg gaaccatccg
     7981 caggcggaca ttcgccgcaa gtgcgccgat tcgcgccaat tgtttttcgt cggcgagcgc
     8041 gacggcacgc cggtggcgag ctgcatggcg ggttacgacg gccaccgcgg ctggatttat
     8101 tacctggccg tggcgcccga actgcggcgc cagggtatcg ccgcccggct ggtgtcgatc
     8161 gccgaagccg aactgcgccg gctcggctgc ccgaagatcg acctgatgat tcgcgacacc
     8221 aaccagcagg tgatcgcgtt ttaccgcgca ctcgg
//
LOCUS       DATCZV010000421         3623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_35735, whole genome shotgun sequence.
ACCESSION   DATCZV010000421 DATCZV010000000
VERSION     DATCZV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35735"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1430)
                     /gene="glp"
                     /locus_tag="VIW27_09550"
     CDS             complement(162..1430)
                     /gene="glp"
                     /locus_tag="VIW27_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5739960.1"
                     /translation="MSDKIVPVPSCADPFGEDLMSLDEALARIENRVVPVDACERLPI
                     RDCLGRVNQEAVHSPLDVPPLPNSAMDGFAVAVSSLRSDAITELDAIGTAYAGKPFDG
                     RVEPGQCVRIMTGALIPDGADTVIMQEQAEVAASGKVRIDADHRAGENIRQAGEDVRK
                     GDVVIDAGARLSPADLGVLASLGIGELQVRRKPVVAFFSTGDELVSVGEPLEPGKIYD
                     SNRYSLYGMLSRMAVDIVDLGVVRDEPEAMRTALENAAAQADLILSTGGVSVGEADYI
                     KPALEEIGSTEFWKIAIKPGRPLTLGQIGSSIFIGLPGNPVAVMVTFSQFVVPAIEVL
                     AGARLKRPALFRARALDRLRKKPGRYEFQRGIATLDDDGEWQVARTGKQGSGILTSMS
                     RANCFIVLPDAVGGIEPGDEVKIQFFDWSL"
     gene            1678..2160
                     /locus_tag="VIW27_09555"
     CDS             1678..2160
                     /locus_tag="VIW27_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5739961.1"
                     /translation="MLKSLNIDPGKCTGCLQCEMACSYEHYGTFNPSRSFIKVFTFHD
                     EGRFAPYTCTQCDEAWCMRACPVDAIGIDASTGAKVVYEDKCVGCKVCTIACPFGTIN
                     YSQASGKVVKCDLCGGDPQCAAACPTSAITYVDADWTGYDRMMASAAKSLPSAEASAQ
                     "
     gene            2177..>3623
                     /locus_tag="VIW27_09560"
     CDS             2177..>3623
                     /locus_tag="VIW27_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5739962.1"
                     /translation="MSWTRKILRVDLAKGTCTSEPLNMEWAKLYLGQRGLASKYLAEE
                     VDPKCDPLGPENKLIMATGPLTGTMASTGGRYSVITKGPLTGAIACSNSGGFFGAELK
                     FAGWDMVIFEGQSDKPVYLLIQDDKAELRSAEHLWGKSAWDTDHAIHATHQDPQMRIA
                     CIGRPAENGCLYAAVMNDRHRAAGRSGVGTVMASKKLKAVAVRGTQGVGNVDDPSAFV
                     AATNDGKSVLAENAVTGQGLPAYGTQVLMNVINGVGGLPARNMRDVGFEGAEHISAEA
                     MAEPRKSDGKPNLTQNNACFGCTIACGRISSIDPTHFSIKDKPEYKISSGGLEYEAAW
                     ALGADTGVDDLDAITYINFLCNEDGLDPISFGATVAAAMEMFEDGVITTEHTGGIELK
                     FGSAEAFVAITEQFIAGGAFSEDLGLGSKRLTEKYGRPELSMTVKGQEFPAYDSRGIQ
                     GMGLTYATSNRGACHLRSYTVASEVLGIPVKT"
BASE COUNT          665 a         1156 c         1177 g          625 t
ORIGIN      
        1 cggtgtgctg ccgtcgaaca ggcgctcgaa gggttcgccg cgcgcgcgca ggcgggcgac
       61 cagctcggcg accgtggcgc cctcgacggg caattccacg gtctcggact tgaggccgag
      121 ttgctcggcc aggctggcga aatacagtac tcgcgccacg actacagcga ccagtcgaag
      181 aactggatct tgacttcgtc gccgggctcg atgccgccga ccgcatccgg cagcacgatg
      241 aagcagttgg cgcggctcat cgacgtcagg atgccggagc cctgtttgcc ggtcctggcg
      301 acctgccatt cgccgtcatc gtccagcgtc gcgataccgc gctgaaactc gtagcgcccg
      361 ggcttcttgc gcaggcggtc gagcgcgcgc gcgcggaaca gggccgggcg cttgaggcgc
      421 gcgcccgcca gtacttcgat cgccggcacc acgaactggc tgaaggtcac catcaccgcg
      481 accgggttgc cgggcaggcc gatgaaaata ctggagccga tctggccgag ggtcagcggt
      541 cggccgggct tgatcgcgat cttccagaac tcggtgctgc cgatttcctc cagcgcgggc
      601 ttgatgtagt cggcctcgcc gaccgagacg ccgccggtgc tgaggatcag gtcggcctgc
      661 gcggcggcgt tctcgagtgc ggtgcgcatc gcctcgggtt cgtcgcggac cacgccgagg
      721 tcgacgatgt cgacggccat ccgcgacagc atgccgtaaa ggctgtagcg gttactgtcg
      781 tagattttgc cgggttctag cggttcgccg accgacacca gctcgtcacc ggtggagaaa
      841 aacgccacca ccggcttgcg cctgacctgc agctcgccga tgccgagcga ggccagcacg
      901 ccgagatcgg ccgggctcag ccgggcgccg gcgtcgatga cgacgtcgcc cttgcgcaca
      961 tcctcgccgg cctggcggat attttcgccg gcacggtggt cggcatcgat acggaccttg
     1021 ccggacgcgg cgacctcggc ctgttcctgc atgatcacgg tgtcggcgcc gtccgggatc
     1081 agcgcgccgg tcatgatgcg cacgcactgg cctggctcga cccggccgtc aaagggtttg
     1141 cccgcgtaag cggtgccgat cgcgtcgagc tcggtgatgg cgtcgctgcg caggctgctg
     1201 acggcaacgg cgaagccgtc catcgccgag ttgggcagcg gcggtacgtc gagcggcgag
     1261 tgcaccgcct cctggttgac ccggccgaga cagtcgcgaa tcggcaggcg ttcgcaggcg
     1321 tccaccggta ccacgcggtt ctcgatgcgc gcgagtgcct cgtccagact catcaggtct
     1381 tcgccgaaag gatcggcaca gctgggaacg ggtacgatct tgtcgctcat cgattttgaa
     1441 aagtacatcc tcgggcgcct aattgaggca tagacgacgg caaaatggta ggggtgaggc
     1501 gatatttttt ctccagaccg cgatcgcggc actgaaagca gcttaaattt gctccggggg
     1561 ctgtaaatgc tcgggcgaca gtggtacttt tacctcttag aaacgcgagg tattccgtcc
     1621 gggttcgcga gcatcgatcc ggggggtgaa gaataacgac acaatcggag gatactcatg
     1681 ctgaaatccc tcaatatcga tcccggcaag tgcaccggct gtctgcaatg cgagatggcc
     1741 tgctcctatg agcactacgg gacattcaat ccgtccagat cctttatcaa ggtgttcacc
     1801 ttccatgacg aaggcagatt cgcgccctac acctgcaccc agtgcgacga ggcctggtgc
     1861 atgcgagcct gccccgtcga tgccatcggc atcgatgctt ctaccggagc gaaagtggtc
     1921 tacgaggaca agtgtgtcgg ctgcaaggta tgcaccatcg cctgtccgtt cggcaccatc
     1981 aactactcgc aggccagcgg caaggtcgtc aagtgcgatc tgtgcggcgg cgacccgcag
     2041 tgcgcggccg cctgcccgac cagcgcgatc acctacgtgg acgcggactg gaccggttac
     2101 gaccgcatga tggcctcggc agccaagagc ctgccgtcag ccgaagcgtc agcacaataa
     2161 acgggggtaa agaaaaatgt cctggacacg caaaattctg agggtagacc ttgccaaagg
     2221 cacctgtaca tccgagccgc tcaacatgga gtgggccaaa ctctatctcg gtcagcgcgg
     2281 gctggcatcg aagtacctcg ccgaggaagt ggatccgaag tgcgatccgc tcgggcccga
     2341 gaataagctg atcatggcga ccggtccgtt gaccggcacc atggcctcga ccggcgggcg
     2401 ctattcggtg atcaccaagg ggccgctgac cggcgccatc gcctgctcca attcgggcgg
     2461 cttcttcggc gccgagctga agttcgccgg ctgggatatg gtgattttcg agggccagtc
     2521 cgacaagccg gtgtacctgt tgatacagga cgacaaggcc gaactgcggt ccgccgagca
     2581 cctgtggggc aagtcggcct gggataccga tcatgccatc catgccactc accaggatcc
     2641 gcagatgcgc atcgcctgta tcggccgccc ggccgaaaac ggctgcctct acgcggcggt
     2701 gatgaacgac cgtcaccgcg ctgccgggcg ttccggcgtc ggcacggtaa tggcgtcgaa
     2761 gaaactcaag gcggttgccg tgcgcggtac ccagggtgtc ggcaacgtcg acgatccgtc
     2821 ggccttcgtc gcggcgacca acgacggcaa gtcggtgctc gccgagaatg cggtcaccgg
     2881 ccagggcctg ccggcgtacg gcacccaggt gctgatgaac gtgatcaatg gcgtcggcgg
     2941 cctgcccgcg cgcaacatgc gtgacgtcgg cttcgagggc gccgagcaca tttccgccga
     3001 ggcgatggcc gagccgcgca agagcgacgg caagcccaac ctgacgcaga acaacgcctg
     3061 ctttggctgc accatcgcct gcggccgcat ttcgtcgatc gatccgactc atttctcgat
     3121 caaggacaaa ccggaataca agatatcctc cggcgggctc gagtacgagg cggcctgggc
     3181 gctgggcgcc gataccgggg tcgacgatct cgacgcgatc acctacatca atttcctctg
     3241 caacgaggac ggcctggatc cgatttcctt cggcgccacc gtggcggcgg cgatggaaat
     3301 gttcgaggat ggcgtcatca ccaccgagca taccggcggc atcgaactca agttcggcag
     3361 cgccgaggcc ttcgtcgcga tcaccgagca atttatcgcc ggcggcgcct tttccgagga
     3421 cctgggactc ggctccaagc ggctgaccga gaaatacggc cgcccggaac tgtcgatgac
     3481 ggtcaagggt caggaattcc cggcctacga ttcgcgcggc atccagggca tgggcctgac
     3541 ctacgcgacg tcgaaccgcg gtgcgtgcca cctgcgcagc tacaccgtgg cctcggaggt
     3601 gctgggcatc ccggtcaaga ccg
//
LOCUS       DATCZV010000422         4376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_194414, whole genome shotgun sequence.
ACCESSION   DATCZV010000422 DATCZV010000000
VERSION     DATCZV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194414"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..558
                     /locus_tag="VIW27_09565"
                     /pseudo
     CDS             1..558
                     /locus_tag="VIW27_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226172.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(555..944)
                     /locus_tag="VIW27_09570"
     CDS             complement(555..944)
                     /locus_tag="VIW27_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739963.1"
                     /translation="MFNPITIPYDAVMLYNVVKLKPGVSVEDAEMAIGEMCNVVKNTY
                     GDDNGGFLGGQVFEYTGFVSDEGSFDPERKSEDHLAIITYWNSFEQHEKSHADQAFRE
                     KFAGLAELSSTTYELGYNMLWQGAPGD"
     gene            1245..2006
                     /locus_tag="VIW27_09575"
     CDS             1245..2006
                     /locus_tag="VIW27_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HEY5739964.1"
                     /translation="MENIESIWQLGLLALAAGALIGALGYRLFAPSLTRADKIRSELE
                     AARQELIDYKASVNSHFDKTSELVNDLTQNYVKVYQHLAEGAQTLGGSRQLNNLLEQQ
                     QGKVLLTVDDELTVEAAQPAARGTAARRPSHETAPAADSSPADDSAEPASEVAAEVAT
                     EETAQTPKDRPAEAAAAMAKEEVADSEQATQEVQQTAGTGSAADADDAAKTSEPVIDV
                     SKIGQGDAKSDKTEAELGTLIPEPGNSEGIKPTRH"
     gene            complement(2064..2425)
                     /gene="ssrA"
                     /locus_tag="VIW27_09580"
     tmRNA           complement(2064..2425)
                     /gene="ssrA"
                     /locus_tag="VIW27_09580"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2579..3103
                     /locus_tag="VIW27_09585"
     CDS             2579..3103
                     /locus_tag="VIW27_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5739965.1"
                     /translation="MFKSIVVAFDGSRHATRALQIGAELAARDGAALGIVYVIDESHF
                     HIAEEMRRMGEVEQVIDPAQKLQINFENAPEAMISNLAQVSGETLRVMQQYAEFLVGQ
                     AARDAVGAGAKQVETSIEEGDPADEIIAWAKDRDADLIVCGNRGLGRWKSLLLGSTSS
                     KITQLSDCSCLTVK"
     gene            complement(3123..4097)
                     /locus_tag="VIW27_09590"
     CDS             complement(3123..4097)
                     /locus_tag="VIW27_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5739966.1"
                     /translation="MLLELVDERRRRGHVRDCHGDLHLGNMALIDGKTRFFDCIEFNP
                     ALRWIDTLSEVAFVAMDLHARGYAGYCWRFLNRYFERSGDFEGIALLRYYFVYRALVR
                     AKVEALRVAQNKPAAGEQRGAFAAAVDYIDLAASWADGHRAGLIMMHGLSGSGKSTVA
                     AQLVERLGAIQLRSDVERKRQFGLGALEASQSATGGGIYTASATAATYQRLRELAQQI
                     TAADFTAIVDATLLTVSQRRPFLELEGAQPIRRVIVDCDAPHAELRRRIAAREGDASE
                     ASLEVLERQLETREPLGAAERELAAVVSVDSGGIDDKTFDLIRARLFD"
     gene            complement(4010..>4376)
                     /locus_tag="VIW27_09595"
     CDS             complement(4010..>4376)
                     /locus_tag="VIW27_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY5739967.1"
                     /translation="ILRGEDSADLTLQAHDHLSISTIPEWNSEWTVTLEGEVRFPGEY
                     RVRRGETLRQVLERAGGLTDEAFPEGSVFLRESLREREQDQIDLTAKIACCWNWLTNA
                     AAAVTFAIAMATCISATWR"
BASE COUNT          850 a         1313 c         1394 g          819 t
ORIGIN      
        1 ccgcctggct actagctcga atggggcggc gaatacgaag actcgatcaa tgcccaggcg
       61 ggtctcgccg gcgcgctgcc ggtgggtttc ctgctgatga tcatcaccag tattttcctg
      121 ttcggcaagc tcaggcagcc cggcatcatc tggctgaccg tgccgctggc gatcatcggc
      181 attaccgccg ggttgctggc caccggcggc gccttcgatt tcatgtcgat actcggcgcg
      241 ctgtcgctgg tcgggctgct gatcaagaac gccatcgtgc tgatcgagga gatcgatcaa
      301 cagatcgaga caggcaagcc gcgctacgag gcgatactgg attcagcggt tagccgcatg
      361 cggccggtga tgctggccgc ggcgactact atcctcggcc tgattccgct actgagcgac
      421 gttttctttg tcaacatgtc ggtgaccatc atggccggtc tcggctttgc atcggtgctg
      481 acgctgatcg tcgtgccgac gctatacgcg atattcttcc gcatcggcaa caacgaaacc
      541 gggcgcggcg cggcctagtc gccgggcgcg ccctgccaga gcatgttgta gccgagttcg
      601 taggtagtcg acgataactc ggcgagcccg gcgaactttt ccctgaaggc ctggtcggcg
      661 tgcgattttt cgtgctgctc gaacgagttc cagtaagtga tgatggcgag gtgatcctcg
      721 gacttgcgtt cgggatcgaa ggagccctcg tcggaaacga aacccgtgta ctcgaacacc
      781 tgaccgccga gaaatccgcc gttgtcgtcg ccgtaggtgt tcttgacgac gttgcacatt
      841 tcgccgatcg ccatttcggc gtcttcgacg ctgacccccg gtttcagttt caccacgttg
      901 tacagcatca cggcgtcgta agggatggtt atcgggttga acatctatca atgcctcgct
      961 atttgcgttt cggttgttga cgagctgcgt taccagatag gtacgataga ggaaatatca
     1021 atgccccata ggcgggaggg caggaggata actgcgtcgg cccggtcagt ccgccggtct
     1081 cggtcgcagt tgggtgaagt gccgtattct taacaggcaa ctgatttgta ggcatttatt
     1141 tgtatcgacg acgggctcgg caaggtgccg gtatgattgc accggcggat attttataat
     1201 cggcgatgga ttggagtccg tttgcagaaa ccagggggaa gattgtggaa aacatcgaat
     1261 cgatctggca gctgggattg ctggcgttgg ccgccggcgc gctgatcggt gcgctcggct
     1321 accggctgtt cgcgccttcg ctgacccggg ccgacaagat caggtccgag ctggaggcgg
     1381 cgagacagga gttgatcgat tacaaggcca gcgtcaacag ccatttcgac aagacctcgg
     1441 agctggtcaa cgacctgacg caaaactacg tcaaggtata ccagcatctc gcggagggcg
     1501 cgcagaccct cggcggcagc cggcaactga acaatcttct cgagcaacag cagggcaagg
     1561 tgttgctgac cgtcgacgac gagctcacgg tcgaagcggc gcagccggcg gctcgcggca
     1621 cggctgcgcg tcgaccgtcg cacgaaaccg cgccagccgc cgattccagc ccggctgacg
     1681 actccgccga acccgcttct gaagtggctg ccgaagtcgc taccgaggag accgcgcaga
     1741 ccccgaagga caggccagcg gaggccgccg cggcgatggc taaggaagag gtggccgata
     1801 gtgagcaagc cacgcaggag gtgcaacaga ccgccggcac tggcagcgcg gccgacgccg
     1861 acgacgcagc gaaaaccagc gagccggtca tcgacgtcag caagatcggg cagggcgatg
     1921 caaaaagtga taagaccgag gccgaactcg gcacgttgat ccccgagccc ggaaacagcg
     1981 agggcatcaa gcccacccgt cactagggcg cggatacccg gcatcgtgaa gttcgcggcg
     2041 cttgccgccg cgaacgcgtc atttggtgga gacggcggga atcgaacccg cgtccgtaag
     2101 ccctctgccg tcggctctac atgcttattc cgggccttga tttagctgcc tgctatccgc
     2161 cggactggaa aaacaaacag cgattccggt acttgtttag tgcttcaccc ccggacgagg
     2221 atccatcacg attccatgaa agcgacgccc gggtctggac gcatggacac ggctccagtc
     2281 ggacggcacc ctaccgggta ttaagcggcg agtgcgtagt tgtcgtcgtt ggcaactata
     2341 agtttgcagg agatttaacg agtttcccta cattctcggc atgcacctcg ggtttcgcga
     2401 cccacgtcga agccagatcg tccccgaata tggaagtata acccagatgg ggccgcgagc
     2461 gcgattttca atcgctagcg ccgtgaccag atgatcgacg cggcgcattg acccgcgtca
     2521 attatccgct gccacggccg tgatgcaatg aatacagcca aagcacggag aagacgccat
     2581 gttcaaatcc atcgttgttg catttgacgg ttcgcgtcac gccactcgcg cgttacagat
     2641 cggggcggaa cttgccgcgc gggacggcgc cgcgctgggt atcgtctacg tgatcgacga
     2701 gtctcatttt catattgccg aggaaatgcg ccgcatgggc gaggtcgagc aggttatcga
     2761 cccggcgcaa aagctgcaga tcaatttcga aaacgccccc gaggcgatga tcagcaacct
     2821 ggcccaggtc agcggggaaa cgctgcgcgt gatgcagcag tacgctgaat tcctcgtcgg
     2881 gcaggccgcc agggacgccg tcggggccgg cgcgaagcag gtcgaaacct cgatcgaaga
     2941 gggtgatccg gccgacgaga tcatcgcctg ggcaaaggat cgcgacgccg acctgatcgt
     3001 ttgcggcaac cgcggcctcg ggcgctggaa gagcctgctg ctcggcagta cgtcgagcaa
     3061 gattacccag ctcagcgatt gcagctgcct gaccgtgaag tagtaacgcg gtttcgagcc
     3121 cgctaatcga acaaccgcgc gcggataagg tcgaacgttt tatcgtcgat gccgccgctg
     3181 tcgacgctca ctacggcggc cagttcgcgt tccgcggcgc cgagcggctc gcgggtctcg
     3241 agctgccgtt cgagtacttc caggctggct tccgaagcat ctccctcgcg agccgcgatg
     3301 cgccgtcgca gttccgcatg cggcgcgtcg cagtcgacga tcacgcgccg tatcggctgc
     3361 gcgccttcga gttcgagaaa cggccgccgc tgggataccg tcagcagggt cgcgtcgacg
     3421 atcgcggtaa agtcggctgc agtgatttgc tgcgccagct cgcgaagtcg ctgataggtg
     3481 gccgcggtgg ccgacgcagt gtagataccg ccgccggtcg ccgattggct ggcctcgagg
     3541 gcgccgagcc cgaattgccg cttgcgctcg acgtcggagc gcagctgaat cgcgccgagc
     3601 cgctcgacca gctgtgctgc gaccgtcgat tttccggagc cggacaggcc gtgcatcatg
     3661 atcagcccgg cgcggtggcc gtcggcccag ctggcggcga gatcgatata gtcgacggcg
     3721 gccgcgaacg cgccgcgctg ctcgccggcg gcgggcttgt tctgcgccac gcgcagggcc
     3781 tcgaccttgg cgcgtaccag cgcccggtag acgaagtaat agcgcagcag tgctatacct
     3841 tcgaaatcgc cgctgcgctc gaaatagcgg ttcaggaagc gccagcaata gccggcatag
     3901 ccgcgcgcgt gcagatccat ggcgacgaag gccacttcgc tgagggtatc gatccagcgc
     3961 agtgcgggat tgaattcgat gcaatcgaaa aaacgcgtct tgccgtcgat cagcgccatg
     4021 ttgccgagat gcaggtcgcc atggcaatcg cgaacgtgac cgcggcggcg gcgttcgtca
     4081 accagttcca gcagcatgcg attttcgcgg tcagatcgat ctggtcctgc tctctttccc
     4141 gcagcgactc gcgaaggaat acggaacctt ccgggaacgc ctcatcggtc aggccgccgg
     4201 cgcgctcgag cacctggcgc aaggtttcgc cgcggcgaac gcggtattcg cccgggaagc
     4261 gaacctctcc ttcaagtgtc acggtccact cggagttcca ttcggggatg gtgctgatgc
     4321 tcagatgatc gtgcgcctga agcgtaagat ctgcagagtc ttcgccgcga agaatg
//
LOCUS       DATCZV010000423         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_670377, whole genome shotgun sequence.
ACCESSION   DATCZV010000423 DATCZV010000000
VERSION     DATCZV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670377"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VIW27_09600"
     CDS             complement(<1..205)
                     /locus_tag="VIW27_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5739968.1"
                     /translation="MTMDKVLFGERLRNAREASGMSQKLMASRLGVKSSTVGKWESGK
                     EDPRANRLQMIASLLNVPLLWLLA"
     gene            422..1255
                     /locus_tag="VIW27_09605"
     CDS             422..1255
                     /locus_tag="VIW27_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY5739969.1"
                     /translation="MPSGNDDQNLAIFCDFENIALGVRDARMQKFDINLILERLLVKG
                     TVVFKKAYCDWERYKEFKAVMHEASFELIEIPHTRQSGKNSADIRMVVDALDLCYTKE
                     HIDTFVIISGDSDFSPLVSKLRENNKVVIGVGVKSSTSDLLMNNCDEFIYYDDLARDQ
                     ESSKKRKRRPSPKKKAASKSDTKQHANLDADDERIDEAISLLMETAEALQAERGDREK
                     LWGSMIKQALKRRRPGFTESYYGARSFSDLLEEAERRGLMHLTLDERSGGYLIQNLDT
                     D"
     gene            1470..2453
                     /locus_tag="VIW27_09610"
     CDS             1470..2453
                     /locus_tag="VIW27_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HEY5739970.1"
                     /translation="MAKSQPAEFELPPRPRKDDGGPRRVGFEIEFSGIDLQHTTEAVK
                     TALNANIVSQTAAEVELEAGDLGVFNIEIDWQFLKRKASADGDDRDWLELLSQAAALL
                     VPIEVVCPPIPVSRLAELEPMTDALRRAGAVGTEESLISAYGVHVNTEIPRLDADTLF
                     AYLRAYALLQWWLIDRRPVDLARRISPYIDLYSQTYLKRLFTRRQPTVEQLIDDYLEH
                     NATRNRGLDMLPMLAEIDEQRVRRMVDDPRVKSRPTFHYRLPDCNIEQRDWSLRQPWQ
                     TWLTIEKLADRPTDLDKLARAFLDAERPLIGVNRGEWVEHIDRWLQDRELG"
     gene            2426..3094
                     /locus_tag="VIW27_09615"
     CDS             2426..3094
                     /locus_tag="VIW27_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322270.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY5739971.1"
                     /translation="MAARPRVGVTGNHRRWAPGWWCAALALRLSGAVPERISVRHSPS
                     GRPLAAVVIGGGSDVGPEHYGGDIDARVKADPERDLLEIDWIKRAIRERIPILGICRG
                     AQLINVVMGGRLHQDIREIRVHTRNRPSLLPTKRVHLHEDSQISAIIRRRQIRVNSLH
                     HQAVDLPGEGLRAVGHDRDEIVQAIESESGWPIIGVQWHPEYLFYLPSQLALFRWLLS
                     QAVK"
     gene            3091..3519
                     /locus_tag="VIW27_09620"
     CDS             3091..3519
                     /locus_tag="VIW27_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY5739972.1"
                     /translation="MISYLALFGSAFLAATILPFYSEVLLYALLREGFDPLLLVIVAT
                     LGNTLGAVVNWVLGLYLLHFQDRRWFYFSREQIDKAQRWYRRYGFWSLLLAWMPVGGD
                     ALTLIAGIMKVRLWLFLLLVGTGKALRYVSVVYITDWIHA"
     gene            complement(3564..4238)
                     /locus_tag="VIW27_09625"
     CDS             complement(3564..4238)
                     /locus_tag="VIW27_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEY5739973.1"
                     /translation="MLRVILWKLGIEWAHLKRALWPRQARKVYYFAFGANLCSEVLAQ
                     RRIRVYEAFDHVLHDAELRFSQPGFYRDHGYASADPAVGGRVYGRMYLILESDALRMD
                     YFEGVSFLDAHEKVYREADGLRFFYYRTTRVVGGLKPTREYLDYIVDAYRAMPGVPGD
                     YLHNLQATAVLGDLQPTECTGLFVRHAERWPRCLEGLLLAYERLCLKAVELVWHRSPL
                     QLLIRD"
BASE COUNT          872 a         1365 c         1271 g          747 t
ORIGIN      
        1 cggcgagcag ccacaacaac ggcacgttga gcagactggc aatcatttgc aagcgattgg
       61 cgcgcggatc ttccttgccg ctttcccact tgccgaccgt agaactcttg acacccaggc
      121 ggctggccat cagcttctgg ctcatgccgg aggcttcgcg cgcattcctc agtcgttcgc
      181 caaaaagtac cttgtccatc gtcatattca attcccaaaa ggcgatgcac cctaacaagt
      241 tggcgggtgt ttttcaactt ggcaccgcct tcgggcgccg cggacaagca ctgtgagacg
      301 gcgcacgggc cgcccggcgc ggcgattacg gccggccgca aacaaattac tttgacccga
      361 aatcgggttg tcggttagat tgcacgcatt acgcaaaccc gaccccgccg cggagactga
      421 catgccatcc ggtaacgacg accagaatct cgcgattttc tgcgatttcg aaaatatcgc
      481 gctcggcgtc cgtgacgcca ggatgcaaaa attcgacatc aacctgatcc tcgagcgcct
      541 gctggtgaaa ggaaccgtgg tcttcaaaaa ggcctattgc gactgggagc gctacaagga
      601 attcaaggcg gtgatgcacg aagcctcgtt cgaattgatc gagattccgc atacccgcca
      661 gtcgggcaag aattcggccg acattcgcat ggtcgtcgac gcactcgacc tgtgctacac
      721 caaggaacac atcgatactt tcgtaatcat cagcggcgac tccgattttt caccgctggt
      781 cagcaagctg cgcgaaaaca acaaggtggt gatcggcgtc ggcgtgaaaa gctcgacttc
      841 cgacctgctg atgaacaatt gcgacgagtt catctactac gacgatctcg cccgcgacca
      901 ggagtcgagc aagaaacgca agcgcaggcc ctcgccgaag aaaaaggccg ccagcaaaag
      961 cgacaccaag cagcatgcaa acctggacgc cgacgacgaa cgcatcgacg aggcgatcag
     1021 cctgctgatg gagaccgcag aagcgctgca ggccgagcgc ggcgaccgcg aaaaactctg
     1081 gggttcgatg atcaagcagg cgctgaaacg gcgccgcccc ggctttaccg agagctatta
     1141 cggggcccgc tcgttcagcg acctgctcga ggaagcggag cgccgcgggt taatgcacct
     1201 gaccctcgac gagcgctctg gcggttacct gattcagaat ctcgacacgg attgaattgc
     1261 cgacgccagc aagccctgac accgtcgcat gaggcatcgc cgacgagcga cgcggcatcg
     1321 ctatccgcga tatgcgccat gccggtaaca ccgtcgcgac ggcaagagcc gtgttgtcgt
     1381 tgccggcggg cgacggccgg cgcgctcacc tgctgccaaa attcgtcgcc tgggcgataa
     1441 ttacgcttcg agaccagaga gcgcagcaga tggcaaaatc gcagccagca gaattcgagc
     1501 tccccccgcg gccgcggaaa gacgatggcg gaccccgccg cgtcggtttc gagatcgagt
     1561 tttcaggcat cgatctgcaa cacacgaccg aggcggtgaa aaccgcgctg aatgcgaaca
     1621 tcgtttcgca aactgccgcc gaggtcgaac tcgaagccgg cgacctcggc gtcttcaata
     1681 tcgaaatcga ctggcaattc ctcaagcgca aggcatcggc agacggcgac gaccgcgact
     1741 ggctggagct tctcagccag gccgccgcac tgctggtgcc gatcgaagtc gtgtgcccgc
     1801 cgatcccggt ttcacggctg gccgaactcg aaccgatgac cgacgcactg cggcgggccg
     1861 gcgcggtcgg caccgaagaa tcgctgatct cggcttacgg cgtgcacgtc aataccgaga
     1921 ttccgcggct cgacgcagat accctgttcg cttacctgcg ggcttacgca ctgctgcaat
     1981 ggtggctgat cgaccgccgg ccggtcgacc tggcgcgccg catcagcccg tacatcgacc
     2041 tttactcgca gacttacctg aagcggctgt tcacgcgccg gcaaccaacc gtcgaacaac
     2101 tgatagacga ctacctcgag cacaatgcga cgcgcaaccg tggactcgac atgttgccga
     2161 tgctggccga gatcgacgag caacgtgtca ggcgaatggt cgacgacccg cgcgtcaagt
     2221 cacgcccgac atttcactat cgcttgcccg actgcaacat cgagcagcgc gactggtcac
     2281 tgcgccagcc atggcagacc tggctgacga tcgagaaact cgccgaccgg ccgaccgatc
     2341 tagacaagct ggcgcgcgcc ttcctcgacg ccgagcgccc actgatcggc gtcaaccgcg
     2401 gcgaatgggt ggaacacatc gaccgatggc tgcaagaccg cgagttgggg taaccggcaa
     2461 tcatcgccgc tgggcaccgg gctggtggtg cgcggcactc gcgctcaggc tgtccggcgc
     2521 ggtgcccgaa cgcatcagcg tgcgccattc gccgagcggc aggccactgg cggcggtggt
     2581 catcggcggc ggcagcgatg tcggtccgga acactacggt ggcgacatcg acgccagggt
     2641 caaggcggat ccggaacgcg acctgctgga aatcgactgg atcaagcgag cgatcaggga
     2701 gcgcattccg atactcggca tctgccgcgg cgcgcaactg atcaatgtcg tcatgggcgg
     2761 caggctgcac caggatattc gcgagattcg cgtgcacacc aggaacaggc ccagcctgct
     2821 gcccaccaag cgggtgcatc tccacgaaga ctcgcaaatt tccgcgatca tcagacgccg
     2881 gcagattcgc gtcaatagcc tgcaccacca ggccgtcgac ctgcccggcg aaggcctgcg
     2941 cgcggttggc cacgaccggg acgaaatcgt gcaggcgatc gagagcgaat ccggatggcc
     3001 gatcatcggc gtgcaatggc accccgagta cctgttctac ctgccctcgc aactggcgct
     3061 gtttcgctgg ctgctgtcgc aggctgttaa atgatttcct atctggcgct gttcggctcg
     3121 gctttcctgg cggctaccat tctgccgttt tactcggagg tgttgcttta cgcgctgctg
     3181 cgcgagggct tcgacccgct gctgctggtg atcgtcgcca cgcttggcaa tacgctcggc
     3241 gcggtcgtca actgggtact cggtctctac ctgctgcatt tccaggaccg ccgctggttt
     3301 tacttttcgc gcgagcagat cgacaaggcg cagcgctggt atcggcgcta cggcttctgg
     3361 tcgctgttgc tggcctggat gccggtcggc ggcgatgcgc tgaccctgat cgccggcatc
     3421 atgaaagtgc gcctgtggct attcctgctg ctggtgggca ccggcaaggc gctacgctat
     3481 gtttcggtgg tctacatcac cgactggatt cacgcctgag cctgccgcgc ccggtcgacg
     3541 cgtcgagcct gcgcatccgc gcttcaatcc ctgatcaata attgcagcgg cgaacgatgc
     3601 cagaccagtt ccacggcctt gaggcagagc cgctcgtagg ctaacagcaa tccctccagg
     3661 cagcgcggcc agcgctcggc gtggcgcacg aaaagaccgg tgcactcggt aggctgcagg
     3721 tcccccagca cggcggtcgc ctgcaggtta tgcagataat caccgggcac gccgggcatc
     3781 gcgcggtaag cgtcgacgat gtagtcgagg tactcgcgcg tcggcttgag gccgccgacg
     3841 acacgcgtcg tgcgataata gaaaaaccgc agaccgtcgg cttcgcgata caccttttcg
     3901 tgcgcatcga gaaacgatac gccctcgaaa taatccatgc gcaacgcatc gctctccagt
     3961 atcaggtaca ttctgccgta aaccctgccg ccgacggccg gatcggccga tgcgtaaccg
     4021 tggtcgcgat aaaagccggg ctgactgaag cgcagctcgg catcgtgcag cacgtggtcg
     4081 aaagcctcgt agacccgaat gcgccgctgc gccagcacct cgctacaaag gttggcgccg
     4141 aacgcgaagt agtaaacctt gcgcgcctgc cgcggccaca gcgcccgttt caggtgcgcc
     4201 cactcgatac ccagtttcca caggatgacg cgcaacactg cttattcggc caggc
//
LOCUS       DATCZV010000424         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_405501, whole genome shotgun sequence.
ACCESSION   DATCZV010000424 DATCZV010000000
VERSION     DATCZV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405501"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VIW27_09630"
     CDS             complement(<1..335)
                     /locus_tag="VIW27_09630"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY5739974.1"
                     /translation="MQFRDEKQTDAKTPAARPRIIRSSVLNKTLLSHWVSLSTQRRQG
                     WWLWSMNPLSILLMPLSMLFLVFVKLRLTAYRLRLLRSRRPPVTTVVVGNITLGGNGK
                     TPIVIALYH"
     gene            complement(324..2069)
                     /gene="msbA"
                     /locus_tag="VIW27_09635"
                     /pseudo
     CDS             complement(324..2069)
                     /gene="msbA"
                     /locus_tag="VIW27_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
     gene            complement(2101..2517)
                     /locus_tag="VIW27_09640"
     CDS             complement(2101..2517)
                     /locus_tag="VIW27_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5739975.1"
                     /translation="MNFYHRQAEEEVSINLTPLIDVVFLLLIFFMVSTTFDTTSQLEI
                     KLPEASENPAPEPSRQLNLLIDADGSFFVNARELTGTDSATLRAALVRALEGQRLPIV
                     IQSDANSPVQSLVTAMDVVGQLGLKQVSIATTRPIE"
     gene            complement(2519..3136)
                     /locus_tag="VIW27_09645"
     CDS             complement(2519..3136)
                     /locus_tag="VIW27_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5739976.1"
                     /translation="MYELVQAGGWLMIPILLCSVVAAAIIIERTWTLRRKKVIPEKLL
                     TGIWNLLNNNSLTDQHIAEIERGSPLGKVLAAGLINRHLTRDLVRESIEETGRHVVHE
                     MERFMNTLGTISTITPLLGLLGTVIGMIRVFTAITVIGVGDPGQLAGGISEALITTAA
                     GLTIAIPSLIFHRHLKRKIDDLVVAMEQEAMKLVEFLHGDRKQLC"
BASE COUNT          660 a          948 c          948 g          639 t
ORIGIN      
        1 aaatggtaga gcgcgatcac gatcggcgtc ttgccgttgc cgccgagggt gatattgccg
       61 accactaccg tggtcaccgg cgggcgtcgc gacctgagca ggcgtagccg gtaggcggta
      121 agcctgagct tgacgaaaac gagaaacagc atcgacagcg gcatcagcag gatcgacaat
      181 ggattcatcg accacagcca ccagccctgg cgacgctggg tcgacaggct gacccagtgc
      241 gacaacaggg tcttgttgag caccgacgaa cgaatgatgc gcggccgcgc ggcgggcgtt
      301 tttgcgtcgg tttgcttttc gtcgcgaaac tgcatcgagt gcaacttggc gtaaatgcct
      361 tcgtgggcga gtaattcccg gtgactgccc tgctcgacta tcttgccctg gtccatgacc
      421 aggatatggt cggcatgctc gatggtcgac agccgatgcg cgatgaccag cgtggtgcgg
      481 ttttccatca ggtgttccat ggcgtgctgg atgtgacgct ccgactcggt atccagcgcc
      541 gaagtcgcct cgtcaaggat caggatcggc gcgtccttga gcagtgcgcg ggcgatggcg
      601 attcgttgcc gctgcccgcc cgataacagc accccgcgct caccgacctc ggtatcgagg
      661 ccgtcgggca tggtttcgat gaattcccag gcatgggcct gcttcgcagc ctcgatgatc
      721 tgctgctcgt cgaccttgtc gacgatgcca taggcgatgt tgttgcgaat cgtatcgttg
      781 aacaggcgca cgtcctgccc gacataggcg atctgccgcc gcagatcgga gattcggtag
      841 tggtcgaggc gttcgccatc gagcaggatt tcgccgttgt cacactcgta gagccggggt
      901 atcaggttga cgagactcga cttgccgctg cccgagcggc cgacgatggc cacggtctgg
      961 ttgggctcga cctcgaaact gatgccgtcg agtacgcgtt gctcgctgcg ccggtaacag
     1021 agtaccacgt cgttgaattg cagccgtccc tcggcccgct gcaattgcag ttggccctca
     1081 tcgcgctcgc tgtcgcgcga agtcagttcg aacacgctct cgccggcggc gatgcctttt
     1141 tggatgttgg cgttgatcga cgacagtacg cgcatcggtg ccagcagcat ggtcatcgcg
     1201 aacaaaaacg agacgaaatc gccggtagta atgattccct gcaccgattc ggaggtcgcg
     1261 aaggcaacga tcgccgcaaa cgcgatcgcc accaccagct ggatcagcgg catgctgacc
     1321 gcctgtgcga ccgccatctt catgtgcagc ctcaggttgg tagcgttgat ctggtgaaac
     1381 ttgtcgcgtt cgaaatcgcc accgccgaag atcttgacga tgcgattggc atcgatgact
     1441 tcctgggcga cctgggtgac atcgcccatc gaacgctgaa tgttgcggct gatcatgcga
     1501 aaacgccccg atatcctgac gacgatcaga aacacgaccg gcccggcgat gaggaatatc
     1561 gccgtcagct gccagttgag ccagaccatg taaccgagca gcacgatgat tcgcaggctg
     1621 tcctggatga agaccgtcag cgacgaggaa acggatctgg cgacctgctc gacatcgtag
     1681 gaaaagcgcg ccactatcga tgccgtcgat gactggtcgt actcgtcgct ggtcagggtc
     1741 agcaatttct cgaacatcat tttgcgcagc gaattgatga ccctgcggcc aatgtagtcc
     1801 atgcaatagc tgcgcatgaa caccgcgaac atacgcgcca cgaaaatcag cacgatagcg
     1861 atcggtatca actggatgat ctgcggatcg cggtcgatga agccctcgtc catcaacggc
     1921 ttcatcaggt aggcgaaggc ggcatccgag gccgcgaaaa taatcatgcc gaggaccgcg
     1981 atcagaaaat acagcttgtg ctcgaaagct atcgacaaca gggcgcgata ggctttgacc
     2041 ccggtgaggt tgctttgaat cggattcatc ggactggttt gcgaatacgc agcatgcgga
     2101 ttactcgatc ggccgcgtgg tggcgatcga gacctgcttc aggcccagtt gcccgaccac
     2161 gtccatcgcc gttaccagcg actgcaccgg cgaattcgca tccgactgga tgacgatcgg
     2221 cagccgttgg ccttccagcg cgcgcaccaa cgcggcacgc agcgtggcgc tgtcggtacc
     2281 ggtcagctcg cgcgcattga cgaagaaact gccgtcggca tcgatcagca gattcaactg
     2341 gcgcgacggc tcgggcgccg ggttttcgct cgcctcgggc agcttgatct cgagctgcga
     2401 agtggtgtcg aaggtcgtcg ataccatgaa aaagatcagt agcaggaaca ccacgtcgat
     2461 cagtggcgtc aggttgatac tgacctcttc ctcggcctgg cggtgataga aattcatctc
     2521 agcaaagttg cttgcgatcg ccgtgcagga actcgaccag cttcatcgct tcctgctcca
     2581 tggccaccac caggtcgtcg atcttacgct tgagatggcg gtgaaaaatc aggctcggga
     2641 tggcgatggt cagccccgcg gcggtggtga tcaaggcctc ggagatcccg ccggccagct
     2701 gcccggggtc gccgacaccg atcacggtaa tcgcggtaaa tacgcgtatc ataccgatca
     2761 ccgtgcccag caggccgagc agcggggtaa tcgtcgaaat ggtgcccaga gtgttcataa
     2821 aacgctccat ctcgtgaacc acgtgccgcc ccgtctcctc gatactctca cgcaccaggt
     2881 cacgcgtcag gtgacgattg atcaaccccg cggcgagcac cttgccgagc ggcgaacccc
     2941 gctcgatttc ggcgatgtgc tgatcggtca gcgaattgtt gttgagcagg ttccagatgc
     3001 cagtcagcaa cttttcagga atgaccttct tcctgcgcaa ggtccatgtg cgctcgatga
     3061 taatcgccgc cgcgaccacc gaacacagca gaatcggaat catcagccag ccaccggcct
     3121 ggaccagttc gtacacgcgg attacctcga tttcaaatgc cgcacatcat acttgcaccg
     3181 caggtttttt accat
//
LOCUS       DATCZV010000425         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_405547, whole genome shotgun sequence.
ACCESSION   DATCZV010000425 DATCZV010000000
VERSION     DATCZV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405547"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="VIW27_09650"
     CDS             complement(<1..1856)
                     /locus_tag="VIW27_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5739977.1"
                     /translation="MSRQWMKALQQASLLDGGNGAWLESLYESWQEDPASVDQQWRPF
                     FEQLPRVNGHVQDEVRHSELRAEFSRLTGRLRGHRIARLTGTRLLQERKQVRVLQLIN
                     AYRFRGHEHATTDPLRRHSPEPVEELELAYHELSEADFGSRFPTGSMVGFDDAATLED
                     ILVALNRTYCESVGAEYMHIPETLEKRWIQQRLESVQSHPAVPDRKRRWLLDRLTAAE
                     GIERYLHSKYVGQKRFSLEGGESLIPMLDTLVQEAGVQGVKEIVIGMSHRGRLNVLVN
                     ILGKSPKDLFSEFEGVQRKDLQAGDVKYHQGFSSNIQTPGGLLHVALAFNPSHLEIVS
                     PVVHGSVRARQQRYADRTGDRVMPIVIHGDSAFAGQGVVMETFNMSGLRGYTTRGTVH
                     VVINNQIGFTTSHRDDARSTLYCSDVAKMVNAPIFHVNGDDPEAVYFVTRLALDYRMT
                     FNKDVVVDLVCYRRHGHNEADEPSATQPMMYREIKALTPTRGQYATSLEKAGVIDEGE
                     AQQRVEAYRGLLDAGSSVTPDVVNGEAKQELHLVDWHPYIGRVWDEQVDTAVSLAVLQ
                     KLSKKLLALPTGFELHPRVAKILDDRRKMAAGALPVDWGCAEALAMGSLLQEG"
     gene            complement(2148..>2363)
                     /locus_tag="VIW27_09655"
     CDS             complement(2148..>2363)
                     /locus_tag="VIW27_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5739978.1"
                     /translation="GYLVPVSIFECICHRVIVFTFSICPFLPAVNGSFCPIYSASDRQ
                     FAGTCRWPAWQAGIATVARLINKLDQA"
BASE COUNT          471 a          737 c          698 g          457 t
ORIGIN      
        1 ccttcctgca acagcgaacc catggccagc gcctcggcac aaccccagtc gaccggcagc
       61 gcgccggccg ccatcttgcg gcgatcatcg agtatcttcg ccacgcgcgg gtgcagctcg
      121 aagccggttg gcagggccag cagttttttc gaaagcttct gcagcactgc cagcgacacg
      181 gcagtatcga cctgctcgtc ccagacgcgg ccgatgtagg gatgccagtc gacaaggtgc
      241 agttcctgct tcgcctcgcc attgacgacg tccggcgtga cgctgctgcc cgcgtccagc
      301 aggccgcggt aagcctcgac gcgctgctgc gcttcacctt cgtcgatcac gcccgctttt
      361 tcgagcgatg tcgcatactg cccgcgggtc ggcgtcagcg ccttgatctc gcggtacatc
      421 atcggctggg tcgcggacgg ttcgtccgcc tcgttgtggc cgtggcgtcg atagcagacg
      481 agatcgacga ccacgtcctt gttaaaggtc attcggtaat ccagcgccag ccgggtgacg
      541 aaatacaccg cctcgggatc atcgccgttg acatgaaaaa tgggagcatt gaccatcttc
      601 gccacgtcgc tgcagtacag ggttgagcgg gcgtcgtcgc ggtgactggt ggtgaaaccg
      661 atctggttgt tgatgacgac atgcaccgtg ccacgcgttg tgtaaccgcg cagtccggac
      721 atgttgaagg tttccatcac cacgccctgc ccggcaaatg ccgaatcgcc gtgaatgact
      781 atgggcatca cccggtcgcc ggtacggtcg gcatagcgct gctggcgtgc gcgcaccgaa
      841 ccatgcacga ccggggaaac gatctcgagg tgcgagggat tgaacgccag cgcgacatgc
      901 agcaaaccgc ctggcgtctg aatattcgac gaaaaaccct ggtgatactt gacgtcgccg
      961 gcctgaaggt ccttgcgttg tacgccctcg aattcggaaa acaggtcctt cggcgacttg
     1021 ccgagaatat tgaccagcac gttcagccgg ccacggtgcg acatgccgat gacgatttcc
     1081 ttcacgccct gcacgcccgc ctcctggacc agcgtgtcca gcatcgggat caggctttca
     1141 ccaccctcca gcgaaaaacg tttctggccg acgtacttcg agtgcaggta acgctcgatg
     1201 ccctcggccg ccgtcagccg gtcgagtagc cagcggcgtt tccggtccgg tacagccggg
     1261 tgcgactgca cggattccag ccgctgctgt atccagcgct tctccagcgt ctcgggaatg
     1321 tgcatgtact cggcgcccac cgactcgcag taggtgcggt tcagggcaac caggatgtcc
     1381 tcgagggtgg ccgcatcgtc gaagccgacc atggaaccgg tgggaaaacg cgagccgaaa
     1441 tcagcctcgg aaagttcatg gtaagcgagt tccagctctt cgactggctc gggtgaatga
     1501 cgacgcagtg gatcggtggt ggcgtgttcg tgaccgcgga agcggtaggc gttgatcagc
     1561 tgcagcacgc gcacctgctt gcgctcctgc agcaggcgcg tgcccgtgag gcgcgcaatg
     1621 cgatggccgc gaagccggcc ggtcagccgg gaaaactccg cacgcagttc cgagtggcgc
     1681 acctcgtcct ggacatggcc gttgacgcgc ggtaattgtt cgaaaaaggg gcgccactgc
     1741 tggtcgaccg aggcgggatc ttcctgccag ctttcgtaga gtgactcaag ccatgcgccg
     1801 ttgccgccgt caagtagcga cgcctgctgc agtgccttca tccattgcct gctcactcgt
     1861 taccgactcc cggccagcgt gattgcagaa tgcagctgaa atcccctgct gccaaaccct
     1921 cctgccttat caaggactat cgttggcccg cggagtttct tgaatacatg aatcgccgtt
     1981 tcacaccgcc gtatggcagg ccgcacgtct accgcgatac taatcacgct ccagcggact
     2041 gtaaaccgta ccgacgatga aaaatatctc caattttcaa gctgtcctgc cgatacaggg
     2101 gctacaagtt gccgggagtc aacgtgaacc tgtgcaacca acgggattta ggcctgatcg
     2161 agcttgttaa tcagcctggc gactgtcgct atccccgcct gccaggcagg ccatcggcac
     2221 gtgccggcaa actgccggtc actggcactg tagatcgggc aaaaactgcc gttaacagct
     2281 ggcagaaacg ggcaaattga aaaagtaaaa acaatcaccc tatgacagat gcactcgaaa
     2341 atgcttactg ggacaaggta gcc
//
LOCUS       DATCZV010000426         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_194667, whole genome shotgun sequence.
ACCESSION   DATCZV010000426 DATCZV010000000
VERSION     DATCZV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194667"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..2324)
                     /locus_tag="VIW27_09660"
     CDS             complement(81..2324)
                     /locus_tag="VIW27_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEY5739979.1"
                     /translation="MPHSTYLPAKMGTAVVEPTGEFEAGSFEEFTLTYTAGYFGIDDT
                     GSLKIVHRFASDMGRPQFDDPVAPNYVTVEASNGALLHVEYDIKRNIRPWDKTLYIKV
                     VRGFLREGDRIIVRFGDRRQGSPGMRLQTFCEYSFEFRVLVDAIATYNYVELPEQPEI
                     AIVPGPPVLYKAILPTLRRRKQAFRLCLKGEDRWGNPSNRCDRRFELHASLPLRGLPA
                     EVVFKDGEFACIVDDLQADETGDLIIELRDDDGHLVARSNPCRVVDDAPLLPYWGDLH
                     GQSEETIGTNSARDFYEFARDKAFLDICVHQGNDFQITTKFWQHLNELSAEFTEDQRF
                     IVFPGWEWSGNTGLGGDRNVLHMREGRPIHRSSHALVDDLSDIDTDANSANDLFAALK
                     DEDCTVFAHIGGRYADIKVAHDQRIEKGVEVHSAWGTFEWLLQDAFEQGYRVGILSNS
                     DGHKGRPGASHPGATKFGAYGGLTCVLAPSFSRAGIMEGLRRRHYYGTTGSRVLLDTR
                     VEFAADAELFDDDPRLGEVGSRTVGSAMMGDILRCGEREVKFVIDVHSTAPIERIDIR
                     NGLETLEVYRPYDEEQLGRRIRVIWEGSEYRGRGRETNWDGHAELVDNRFETLQPINR
                     YNIDRRFEQTSPERVEWSAITTGGFGGFEAMLQDSTAGTLNIDTALIKQRIAVSDIGR
                     DELMFENGGIERRIRVFRLPDSNPHKSLRIERSIPLRDDGDNALYVRVTHEDGHYSWS
                     SPIYLFR"
     gene            2519..3388
                     /locus_tag="VIW27_09665"
     CDS             2519..3388
                     /locus_tag="VIW27_09665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY5739980.1"
                     /translation="MDYLAVLNASPIGMLLVDPRFDIVFANAKAESMFGYGDGELNGL
                     SVNCLVPEHMRAAHSGLMSDFFSQHGSRGLSKGRYLNVLHRSGELIKVEIGLMPVKVE
                     GSEYILVSALEIANQILNVAAHKDALTGLPSRGLFIELARNLRSLAIREAVALSLLFI
                     DLDEFKQVNDSFGHQAGDRLLGSIANILEKNRRRNDIVGRYGGDEFVMCLYDVSDCAA
                     AEKIARSIIDDIETVAATELDHLQVSASIGQLFLVSPGDLSLEQSLEAADELMYRAKS
                     AGKGTVLSREIGS"
BASE COUNT          650 a         1083 c         1117 g          679 t
ORIGIN      
        1 atcgaggcgt gccgcacgga gaccgtaaac ctcggcgccg aggcacggcg tcagcgggcg
       61 tatgtcgagg gcgtcgccca tcaacgaaac aggtagatcg ggctcgacca gctgtaatgg
      121 ccgtcctcgt gggtcacgcg cacgtagagc gcattgtcgc cgtcgtcgcg tagcgggatg
      181 ctgcgttcga tgcgcagtga tttgtgcgga ttgctatcgg gcaggcggaa gacgcgaatg
      241 cggcgctcga tgccgccatt ttcgaacatc agctcgtcgc ggccgatgtc gctgaccgcg
      301 atccgctgct ttatcagggc ggtgtcgata ttcagcgtgc cggccgtcga atcctgcagc
      361 atggcttcga aaccaccgaa accgccggtg gtgatcgcgc tccattcgac acgttcggga
      421 ctggtctgct cgaagcgtcg gtcgatgttg tagcgattga tcggctgcag cgtctcgaag
      481 cggttgtcga cgagttcggc gtgcccgtcc cagttggttt cgcggccgcg gccgcgatac
      541 tcggaacctt cccagatgac gcgaatccgg cgccccagct gctcctcatc gtaaggccgg
      601 tagacttcca gggtctcgag accgttacga atatcgatac gctcgatcgg cgcggtcgaa
      661 tgcacgtcga tgacgaattt cacctcgcgc tcgccgcagc gcagaatatc gcccatcatc
      721 gccgagccca ccgttcgcga gccgacctcg ccgagccgcg ggtcgtcgtc gaacagctcg
      781 gcatcggcgg cgaattcgac gcgggtgtcg agcagcacgc ggctgccggt ggtgccgtaa
      841 tagtggcgcc ggcgcaagcc ttccatgatg ccggcgcggc tgaacgacgg cgccagcacg
      901 caggtcagcc cgccgtaggc gccgaacttg gtggctcccg ggtgcgaggc gcccggtcgg
      961 cccttgtggc cgtcggaatt cgacaggatg ccgacccggt aaccctgctc gaaagcatcc
     1021 tgcaacagcc attcgaaagt gccccaggcc gagtgtacct cgacgccttt ctcgatgcgc
     1081 tgatcatgcg ccaccttgat gtccgcgtaa cggccgccga tatgcgcgaa caccgtgcaa
     1141 tcctcgtcct tcagggccgc gaacaggtcg ttggccgagt tggcatcggt gtcgatatcc
     1201 gacaggtcgt cgaccagcgc atgcgacgag cgatgaatgg gcctgccttc gcgcatgtgc
     1261 agcacgttgc gatcgccgcc cagcccggta ttgccggacc attcccagcc cgggaagacg
     1321 atgaatcgct ggtcctcggt aaactccgcc gacagttcgt tgaggtgctg ccagaacttg
     1381 gtggtgatct ggaaatcgtt gccctggtgc acacagatat cgagaaacgc cttgtcgcgg
     1441 gcgaactcgt agaaatcacg cgccgagttg gtgccgatgg tttcctccga ctggccgtgc
     1501 agatcgcccc agtagggcag caggggcgcg tcgtcgacga cacggcaagg gttcgaacgg
     1561 gccaccaggt ggccgtcgtc gtcgcgtagt tcgatgatca ggtcgccggt ctcgtctgcc
     1621 tgcaggtcgt cgacgatgca ggcgaattca ccgtctttga acacgacctc ggcgggcagg
     1681 cctcgcagcg gcaggctggc gtgcagctca aatcggcggt cacagcgatt cgatggatta
     1741 ccccagcggt cctcgccctt caggcacagg cgaaacgcct gcttgcgccg ccgcagcgtc
     1801 ggcaggatgg ccttgtacag taccgggggg cccggcacga tcgcgatctc tggttgctcg
     1861 ggcaattcga cgtagttgta ggtcgcgatc gcgtcgacca gtaccctgaa ttcaaagctg
     1921 tactcgcaaa aggtctgcag gcgcataccc ggcgaaccct ggcggcggtc gccgaaacgc
     1981 acgatgattc gatcgccctc gcgcagaaag ccgcgcacca ccttgatata gagcgtcttg
     2041 tcccaagggc gaatgttgcg cttgatgtcg tactcgacat gcagcagcgc gccgttgctg
     2101 gcttcgacgg tgacgtaatt cggcgccacg gggtcgtcga attgcggccg gcccatgtcg
     2161 gaagcgaagc gatgaacgat tttcagcgag ccggtgtcgt cgatgccgaa atagccggcg
     2221 gtgtaggtca gggtgaattc ctcgaaggat ccggcttcga attcgcccgt cggctcgacc
     2281 acggcggtcc ccatttttgc gggcagatag gtggagtggg gcactgtctg atcttcctcg
     2341 gcggccggcg agccatcggc ccgattctag caatgccggc ggctaagcca caagcgaggg
     2401 acaccgcgcg ccggcctcgt gaagggctgg ccgtgttgca tcaccttgtg gataaatgcc
     2461 aaaaaaacct tgtggcggct atacttgcgc tactgagttc gattagcgga taaatcttgt
     2521 ggattatctc gcggtactga atgcttcccc gatcggcatg ctgctggtcg atccgcgatt
     2581 cgatatcgta tttgccaacg ccaaggccga gtccatgttc ggttacggcg acggcgagtt
     2641 gaacggcctg tcggtcaatt gcctggtgcc ggaacacatg cgcgcggcgc acagcggatt
     2701 gatgtccgat tttttcagcc agcacggctc gcggggattg tcgaaaggac gctacctcaa
     2761 tgtgttgcac cgtagcggcg agctgatcaa ggtcgaaatc ggcctgatgc cggtgaaggt
     2821 cgagggcagt gagtatatcc tggtctcggc gctggaaatc gccaaccaga tactcaatgt
     2881 cgccgcgcac aaggatgccc tcaccggcct gccgagtcgc ggcctgttta tcgagctggc
     2941 gcgcaacctg cgcagtctgg cgattcgcga agcggtcgca ctgagcctgc tgttcatcga
     3001 tctcgacgag ttcaaacagg tcaacgacag cttcggtcac caggccggcg accgcttgct
     3061 cggctcgatc gcgaacattc tcgaaaagaa tcgccgcagg aacgatatcg tcgggcgtta
     3121 cggcggcgac gaattcgtca tgtgcctcta cgatgtcagc gactgcgctg ccgcggaaaa
     3181 gattgcgcgc tccatcatcg acgatatcga gaccgtggcg gccaccgaac tcgatcacct
     3241 gcaagtcagc gcgagtatcg gccagctttt cctcgtcagc cccggcgacc tgtcgctgga
     3301 acagtcgctc gaagcggccg acgaactcat gtaccgcgcc aagagcgcgg gcaagggcac
     3361 ggtgctgagc cgggaaatcg gcagctgaat ccagtacaat cggcggcgat agatttaccg
     3421 agtccgctcg ttgtccgcat ctcctttcga atccgcgctc gacaccatcg gtaacacgcc
     3481 gctggtcgcg ctcgaccgca tctgcgcggg ctacgacgga cgcattctc
//
LOCUS       DATCZV010000427         6931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_617930, whole genome shotgun sequence.
ACCESSION   DATCZV010000427 DATCZV010000000
VERSION     DATCZV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617930"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VIW27_09670"
     CDS             complement(<1..320)
                     /locus_tag="VIW27_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEY5739981.1"
                     /translation="MSRLLISACLLGEPVRYDGRAKPLQHAGLAALLAEGRVIPFCPE
                     VAGGLPVPRPAAEIVGGGGDAVIDGDARVKTRAADDVTGFFIAGARRALALCRRHDVR
                     VAILT"
     gene            complement(320..1081)
                     /locus_tag="VIW27_09675"
     CDS             complement(320..1081)
                     /locus_tag="VIW27_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HEY5739982.1"
                     /translation="MSDVAKQRLLIALFAVIAIAMLVVPRIPQDPAYHEFADQRAWLG
                     IPNAWNVLSSVVFAWVGLAGLYRLLRQKSLRIRSGFCSAYVIFFAALVLVALGSAYYH
                     WNPTNASLALDRLSMALAFMAFSAILLGERVSPKFARRALLPLLLAAIAGVAYWHYSE
                     LAGRGDLRAYLLLQLMPIILLPFVLLAFDSDYDRNADLWWLLAWYLAAKLCELFDRQI
                     FDVLGMVSGHSLKHIAAGIGGFMLLRHLRLRKPAA"
     gene            complement(1415..1834)
                     /locus_tag="VIW27_09680"
     CDS             complement(1415..1834)
                     /locus_tag="VIW27_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY5739983.1"
                     /translation="MDKSFADIEINHDVAEISKCDLCTNSKCCTYVTQQIDTPRSKYD
                     FEILLWQVSHQGVGAYKDEDGWFVIFESRCQHLLADGRCNIYEIRPAICRTHSNDYCE
                     YDAPAEDGFELYFPDYDSLLRFCKKKFKRWNTGNSPP"
     gene            complement(1827..2738)
                     /gene="hemF"
                     /locus_tag="VIW27_09685"
     CDS             complement(1827..2738)
                     /gene="hemF"
                     /locus_tag="VIW27_09685"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY5739984.1"
                     /translation="MDIEQVRSYLVQLQERISGELQRIDGKAVFVADEWQRAAGGGGR
                     SMVLRDGAVFEQGGVNFSEVFGDNLPASATAQRPELAGRGFRAMGLSLVMHPDNPYMP
                     TSHANVRFFVAEKPGEDAIWWFGGGFDLTPYYGCREDCIHWHRTARQACDPFGAERYA
                     EYKRWCDEYFYLRHRKEPRGIGGLFFDDLNEPDFEQAFAFMRSVGDHYLPAYVPIVER
                     HMNDAFGEREKKFQWYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPVVNWRYN
                     YHPPAGSPEAELTDYYLQARDWLNENG"
     gene            complement(2738..3295)
                     /locus_tag="VIW27_09690"
     CDS             complement(2738..3295)
                     /locus_tag="VIW27_09690"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY5739985.1"
                     /translation="MSPWALNRFVRAVSNGAVFAYPTDTVWGLGCHPLIADAVLRLLS
                     IKQRPVDKGLILLASQLEQCLPYLALSSDELRPLTRPAERPTTWLVPAGEFCPPWIRG
                     QHATVAIRITDHPLVARVCAGIDAPLVSTSANRSGRPTARSALLVRRQFGDQLDFIVG
                     GYRTGGNRPSQIKSLAGGQILRSAS"
     gene            complement(3307..5727)
                     /locus_tag="VIW27_09695"
     CDS             complement(3307..5727)
                     /locus_tag="VIW27_09695"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018876614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEY5739986.1"
                     /translation="MASHLVIVESPAKARTIEKYLGRDFKVLASYGHVRDLVPKEGAV
                     ETDNDFKMNYTPVERNQKHVASIKKELKKADSIYLATDPDREGEAISWHLYELLSEKN
                     LMKNKRAYRVAFYEITRQAVSEAIAHPREITMDLVNAQQARRALDYLVGFNLSPLLWR
                     KIRRGLSAGRVQSPALRMIVERELEIEAFNPEEYWSIESDNSIDGQAFGARLFRLAGE
                     KVKQFTITNREQARDAHRRLNEAAQGKLRVDKVEKKQRKRNPAAPFTTSTLQQEAARK
                     LGFGAQRTMRTAQRLYEGIDIGAGQVGLITYMRTDSVTLSNDAIGEIREFIADKFGAN
                     NLPEAPREYKTKSKNAQEAHEAIRPTSARNEPFIIKQHLGDDEFRLYSLIWKRTMASQ
                     MIHATLDTVSVDLACGSDNSFRASGSTIRDPGFMRVYIEDKDDLPKDDNQENLLPSMQ
                     EGDLVDLNEIRTEQHFTEPPPRYSEASLVKALEEYGIGRPSTYASIITTLLDREYVEL
                     ENKRFHPTDVGRVVARFLTQHFKQYVDYDFTAQLEDSLDAISRGEKDWLPLMKEFWSN
                     FKEQVDDKMENVSRDEAVQARQLGTDEKTGKPISVRMGRYGPFVQIGTREDEEKPRFA
                     GLRPGQKMDTITLEEALDLFKLPRELGVSPEGEQISTNIGRFGPYVKYDGKFVSLKVK
                     EGDDPYTITLERALELVAEKKEFDANREIKLFDGTDVKILRGRYGPYITDGIKNARIP
                     KEVEDPASLDLATCQELIEKAPLPRSKRKKVAAKKKAASSKTAKKKSKKKAATKKAAA
                     AKAVAKTG"
     gene            complement(5771..6253)
                     /locus_tag="VIW27_09700"
     CDS             complement(5771..6253)
                     /locus_tag="VIW27_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY5739987.1"
                     /translation="MKEGVIDVLMYIFSSYADPDDKLPEDREGIDADLRAAGFERLEI
                     DKAFAWLDGLTESDGDDGTEQSASATRVLASEERARLDYNAQGFLLFLEQSGVLTPRL
                     REMVINRVMALESQSEVDIEELKWVVMMVLFNSSDEQDENALMHYEDIVFSEQPAVFH
                     "
     gene            complement(6265..>6931)
                     /locus_tag="VIW27_09705"
     CDS             complement(6265..>6931)
                     /locus_tag="VIW27_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY5739988.1"
                     /translation="ITSGLAAGIDAAAHRGALAAGGCSVAVAGCGPERVYPERHRALA
                     DELAGQGAVVSEFPVGTPPRAPNFPRRNRIISGLSLATLVVEAARRSGSLITAGLAAD
                     QGREVFAVPGSIHNPQTRGCHDLIRDGATLVDSVDEVLRELEALSGHEFSRSPPAPVN
                     QSQVLDRAHRRLLEAIGYDPVHCDVIAARSGLTIDQLSSMLLLLELNELIQSAPGGCF
                     VRT"
BASE COUNT         1261 a         2099 c         2164 g         1407 t
ORIGIN      
        1 gtcagtatgg ctacccgcac gtcgtgtcgg cggcacagcg ccagcgcccg ccgcgcgccg
       61 gcgataaaaa acccggtgac atcgtcggca gcgcgagtct ttacgcgcgc gtcgccgtcg
      121 atcaccgcgt caccgccgcc gccgacgatt tccgccgccg ggcgcggtac cggcaggccg
      181 ccggccacct ccggacaaaa cgggatcacg cggccctccg cgagcagtgc tgcgagtccc
      241 gcatgctgta gcggtttcgc ccggccgtcg tagcggaccg gctcgccgag caggcaggcg
      301 ctgatcagca gcctgctcat tacgctgcgg gtttccgcaa acgcaggtga cgcagtagca
      361 tgaaaccgcc gataccggcg gcgatatgct tcaggctgtg cccgctgacc atgcccagca
      421 catcgaagat ttgccggtcg aacagttcgc aaagtttcgc cgccaggtac caggcgagca
      481 gccaccagag gtcggcattg cggtcgtaat ccgagtcgaa cgccaatagc acgaacggca
      541 gcaggattat cggcatcaac tgcagcaaca ggtaggcgcg cagatcaccg cggccggcga
      601 gttcgctgta atgccagtag gcgacgccgg cgattgccgc cagcagcagc ggtagcagcg
      661 cacggcgcgc gaacttcggc gacacccgct caccgagcag aatcgcgcta aaggccatga
      721 aggcgagcgc catcgacagc cgatccaggg ccagcgacgc attggtcggg ttccagtggt
      781 aataagccga gcccagcgcg acgagcacca gggccgcgaa aaaaatcacg taggccgaac
      841 agaatccgct gcggattcgc agggattttt gccgcagcag gcggtagagc cctgcgagcc
      901 cgacccaggc gaataccacg ctggacagca cgttccaggc gtttggaatt ccgagccagg
      961 cgcgctgatc ggcgaattcg tgatatgccg gatcctgcgg aattctcggc accaccaaca
     1021 tcgcgatggc gatgaccgcg aacagggcaa tcagcaggcg ctgtttggca acatcagaca
     1081 tgatcgcaaa accataaacc cgcaacctag cgattggaag catcccgata acaagtttta
     1141 caacgccggg aaacccgggg tcagaacaaa atttccacaa actcttcgaa taatggcgcc
     1201 agcgactggc gaaatccctg cctgcgcgag gatttcgtca tgctttcctc gaagtgacgc
     1261 caatcgggga aatttcgctc tgagcccagg taatcgacag atgtcgcgta atcgatgcct
     1321 gcaatgcaag cccgtttaag cgggcttgcc gaagccgaaa gctacccgaa tcgccaactc
     1381 cgggaaaacg aatcgcgcca cccagaaccg gcaatcacgg tggcgaattc ccggtattcc
     1441 agcgcttgaa ttttttcttg caaaatctca gcagcgaatc gtaatccgga aagtagagct
     1501 cgaaaccgtc ctcggccggc gcgtcgtact cgcagtagtc gttgctgtgg gtgcggcaga
     1561 tggccgggcg aatctcgtag atattgcagc gcccgtcggc cagcaggtgc tggcagcgtg
     1621 actcgaagat cacgaaccag ccatcctcgt ccttgtaggc gccgacaccc tggtgcgata
     1681 cctgccacaa caggatctcg aaatcgtatt tggaccgcgg cgtatcgatt tgctgggtta
     1741 cgtaggtaca gcacttcgag ttcgtgcaca ggtcgcactt gctgatttcg gcgacatcgt
     1801 ggttaatctc gatatcggca aagctcttat ccattttcgt tcaaccagtc gcgtgcttgc
     1861 aggtaataat cggtcaattc cgcctccggg ctgcctgccg gagggtggta attgtaacgc
     1921 cagttgacca ccggtggcag tgacatcagg atcgattcgg tgcggccacc ggactgcagg
     1981 ccgaacagcg ttccgcgatc gtagaccagg ttgaattcga catagcggcc gcggcgatac
     2041 cattggaact ttttttcgcg ctcgccgaag gcgtcgttca tgtggcgttc gacgattggc
     2101 acgtaggccg gcaggtaatg atcgccgacg ctgcgcatga aggcgaaggc ctgctcgaaa
     2161 tccggctcgt tcaggtcgtc gaaaaacagg ccgccgatgc cgcgcggttc cttgcggtgc
     2221 cgcaggtaaa agtattcgtc acaccagcgc ttgtactcgg cgtagcgctc ggcgccgaac
     2281 gggtcgcagg cctgcctggc agtccggtgc cagtgaatgc aatcttcgcg gcaaccgtaa
     2341 taaggcgtca ggtcgaagcc gccaccgaac caccagatgg cgtcttcgcc gggtttttcg
     2401 gcgacgaaaa aacgcacgtt ggcgtgcgag gtcggcatat agggattgtc gggatgcatg
     2461 accagtgaca ggcccatggc gcgaaagcca cggcccgcga gttcgggccg ctgcgcggtc
     2521 gccgaggccg gcaggttgtc gccgaagacc tcggaaaagt tgacgccgcc ctgttcgaac
     2581 acggcgccgt cgcgcagcac catgctgcga ccgccgccgc cggccgcacg ctgccattcg
     2641 tcggcgacga atactgcctt gccgtcgatg cgctgcagtt cgccgctaat gcgctcctgc
     2701 agttgcacca ggtaactgcg gacctgttcg atatccatca gctcgcgctc ctcaggattt
     2761 gtccgccggc aagcgacttt atctggctag ggcggttgcc gccggtgcgg taaccgccga
     2821 cgatgaaatc gagctggtca ccgaattgcc gcctcaccag cagcgcgctg cgcgcggtcg
     2881 gccggccgct gcgattggcg ctggtcgata ccagcggcgc atcgatgccg gcgcaaaccc
     2941 tggccaccag cggatgatcg gtgatgcgga tcgcgaccgt cgcgtgctgg ccgcgaatcc
     3001 atggcgggca gaattcgccg gcagggacca gccaggtggt cggccgctcg gcgggccgtg
     3061 tcagcggccg tagttcgtcg ctgctcagcg ccaggtaggg caggcattgc tcgagctgcg
     3121 aggcgagcag gatcaagcct ttatccaccg ggcgctgctt gatgctaagc agcctgagga
     3181 ccgcgtcggc gatcagtgga tggcaaccga ggccccagac cgtgtcggtc ggataggcga
     3241 ataccgcgcc gttggagacc gcgcgcacga atcggttcag cgcccaggga ctcatgacgg
     3301 cgggtgtcag ccggttttgg cgacggcttt ggcagcagcc gcttttttcg tcgcggcctt
     3361 cttcttgctt ttcttcttcg cggtcttgct gcttgcggcc tttttcttgg cagccacttt
     3421 cttgcgcttc gatctcggta acggcgcctt ttcgatcagt tcctggcagg ttgcgagatc
     3481 caggctggca ggatcctcga cttccttggg gatgcgcgca ttcttgatgc cgtcggtgat
     3541 atagggcccg tagcggccgc gcaggatttt gacgtcggtg ccgtcgaata gcttgatttc
     3601 gcggttggcg tcgaattcct ttttctcggc aaccagctcc agcgcgcgtt cgagggtaat
     3661 cgtgtacgga tcgtcgcctt ccttgacttt cagtgatacg aacttgccgt cgtacttgac
     3721 gtagggtccg aagcggccga tgttggtcga gatctgctcg ccctcgggcg atacgccgag
     3781 ttcgcgcggc agcttgaaca ggtcgagcgc ctcctcgagc gtgattgtat ccattttctg
     3841 accgggccgc aggcctgcga agcgcggctt ttcctcgtcc tcgcgggtac cgatctgcac
     3901 gaacggtccg tagcggccca tgcgcaccga gatcggtttg ccggtctttt cgtcggtgcc
     3961 gagctggcgc gcctgtaccg cctcgtcgcg gctgacgttt tccatcttgt cgtcgacctg
     4021 ctccttgaag ttgctccaga attccttcat caacggcagc cagtcctttt cgccgcgcga
     4081 aatcgcgtct agcgagtctt ccagctgagc ggtgaagtcg tagtcgacgt actgcttgaa
     4141 atgctgggtc aggaaacgcg ccaccacgcg gccgacgtcg gtggggtgaa aacgcttgtt
     4201 ttctagttcg acgtattcgc gatcgagcag ggtggtgata atcgacgcgt aggtcgacgg
     4261 ccggccgatg ccgtattctt ccagcgcctt gaccagcgag gcctcgctgt aacgcggcgg
     4321 cggctcggtg aaatgctgtt ctgtgcggat ttcgttgaga tcgacgagat cgccttcctg
     4381 catcgacggc agcaggtttt cctggttgtc gtctttcggc agatcgtcct tgtcctcgat
     4441 atagacgcgc atgaagcccg ggtcgcgaat cgtcgaaccg ctggcgcgaa aactgttgtc
     4501 gctgccgcag gcgaggtcga ccgacaccgt gtccagcgta gcgtggatca tctggctggc
     4561 catggtgcgt ttccagatca ggctatagag ccggaactcg tcgtcgccga gatgttgttt
     4621 gatgatgaat ggttcgttgc gcgcgctggt cgggcgaatc gcctcgtgcg cctcctgcgc
     4681 gttcttcgac ttggtcttgt attcgcgcgg tgcctcgggc aggttgttgg cgccgaactt
     4741 gtcggcgatg aactcgcgaa tctcgccgat cgcgtcgttc gacagggtaa ccgagtcggt
     4801 gcgcatgtag gtgatcaggc cgacctggcc ggcaccgata tcgataccct cgtagaggcg
     4861 ctgcgcggtg cgcatcgtgc gctgcgcgcc gaagccgagc ttgcgtgcgg cttcctgctg
     4921 tagcgtcgag gtcgtgaacg gcgccgccgg gttgcgcttg cgctgtttct tttcgacttt
     4981 gtcgaccctg agcttgccct gcgcagcttc gttcaggcgg cggtgggcgt cacgggcctg
     5041 ctctctgttg gtgatcgtga actgtttgac tttttcgccc gccaggcgaa acagcctggc
     5101 gccgaaagcc tggccgtcga tgctgttatc agactcgatg ctccagtatt cctcgggatt
     5161 gaaggcctcg atttcgagct cacgctcgac gatcatgcgc agcgccggac tctgcacccg
     5221 gccggccgac aggccgcggc ggatcttgcg ccacaacagc ggcgacaggt tgaagccgac
     5281 caggtagtcg agtgcccgcc gcgcctgttg tgcgttgacc aggtccatgg tgatttcgcg
     5341 gggatgtgcg atcgcctcgg agaccgcctg gcgggtgatt tcgtagaagg cgacgcgata
     5401 ggcccgcttg tttttcatca ggttcttttc gctaagcagc tcgtacaggt gccaggaaat
     5461 cgcctcgccc tcgcgatcgg ggtcggtggc caggtagatc gaatccgctt tcttgagttc
     5521 tttcttgatg ctggcgacgt gtttctggtt acgttcgacc ggcgtataat tcattttgaa
     5581 atcattatcg gtctcgaccg cgccctcctt gggcaccagg tcgcgcacgt ggccgtagga
     5641 agccagaacc ttgaagtccc tgcccaggta tttctcgatg gttcttgctt tggccgggga
     5701 ttcgacgatg acgaggtgac tggccattta acaatgctga ttcacgagtt cgatcgataa
     5761 cgcaccggta ctaatgaaag accgccggtt gctctgaaaa aacgatatct tcgtagtgca
     5821 tcaatgcgtt ttcatcctgt tcgtcggagc tgttgaacaa aaccatcatc acgacccatt
     5881 tcaattcctc gatatcgacc tcggactgcg attcgagggc catgacgcgg ttgattacca
     5941 tttcgcgcaa ccgcggcgtg agcacgccgg attgctcgag aaacagcaga aatccctgtg
     6001 cattatagtc cagccgcgcg cgttcttcgc tggccagcac gcgggtcgcg ctcgcggact
     6061 gctcggtgcc atcgtcgcca tcggactcgg tcagaccatc gagccaggca aaggccttgt
     6121 cgatttccag gcgctcgaat ccggcggcac gcagatcggc gtcgatgcct tcacggtcct
     6181 cgggtaactt gtcgtcgggg tccgcgtagc tcgaaaaaat atacatcaga acgtcgataa
     6241 cgccttcttt catagatact ccgattaagt cctgacgaag cagccgccgg gtgccgattg
     6301 aattaattcg tttaactcga gtaatagtag catggatgaa agctgatcga tggtcaagcc
     6361 gctgcgggcg gcgatgacat cgcagtgaac cgggtcgtag ccgatcgcct cgagcagccg
     6421 ccgatgcgcc cgatcgagga cttgcgactg gttcacgggg gcaggcggcg atcgcgaaaa
     6481 ttcgtgaccg gacagcgctt cgagttcgcg caggacttca tcgaccgagt ccaccagggt
     6541 cgcaccgtcg cgaatcaaat cgtggcagcc gcgggtttgc gggttgtgaa tcgaacccgg
     6601 aaccgcgaat acctcgcgcc cctggtcggc ggcgagcccg gcggtaatca gcgagccgct
     6661 gcggcgcgcg gcctcgacca ccagggtggc cagcgacaat ccgctgatga tgcggttgcg
     6721 gcgcgggaag ttcggcgccc gcggtggcgt gccgaccgga aattcgctga cgacggcccc
     6781 ctgtcccgcc agctcgtcgg cgagggcgcg atgccgctcg gggtagacgc gctcgggacc
     6841 gcacccggcg accgcgacgc tgcacccgcc ggcggccagt gcgccccggt gcgcggccgc
     6901 gtcgataccg gcagcgagcc cgctggtgat c
//
LOCUS       DATCZV010000428         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_511794, whole genome shotgun sequence.
ACCESSION   DATCZV010000428 DATCZV010000000
VERSION     DATCZV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511794"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="hemE"
                     /locus_tag="VIW27_09710"
     CDS             complement(<1..1025)
                     /gene="hemE"
                     /locus_tag="VIW27_09710"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY5739989.1"
                     /translation="MPELKNDRLIRALLRQPTDTTPVWIMRQAGRYLPEYRATREQAG
                     SFLDLCKNTELACEVTMQPLRRFDLDAAILFSDILTIPEAMGLGLYFATGEGPKFERP
                     LRRAADVARLGVPDPADSLRYVIDAVSLIRRELDGRVPLIGFSGSPWTLSTYMVEGGS
                     SKEYQYIKGMMYGDPQTLHRLLDTLAKSVITYLNAQIEAGAQAVMVFDTWGGVLSPQT
                     YREFSLAYMQQIVDGLKREQDGQPVPVTLFTKGGGQWLEEIAATGCNAVGLDWTVDID
                     NARERIGDRVALQGNLDPCVLYADPQTIRAQASDIITRFGNHPGHVFNLGHGIHQAVD
                     PDRLGVLV"
     gene            complement(1206..1658)
                     /locus_tag="VIW27_09715"
     CDS             complement(1206..1658)
                     /locus_tag="VIW27_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY5739990.1"
                     /translation="MPDSLKSQLQADMKSSMKSGDKARLGVIRLMLSAIKQVEVDERI
                     ELDDTRIIAVLDKMVKQRRESISQFASAGRDDLTAIEQAELEIIGEFMPEALSEAEIE
                     ALIEQSLAATGASSIRDMGKVMGMLKPQLQGRADMGQVSQLIKSRLSA"
     gene            complement(1669..1884)
                     /gene="rpsU"
                     /locus_tag="VIW27_09720"
     CDS             complement(1669..1884)
                     /gene="rpsU"
                     /locus_tag="VIW27_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY5739991.1"
                     /translation="MPSVKVKDNEPFDFALRRFKRSCEKAGILTETRRRQHYEKPTAV
                     RKRKSAAAVKRNFKRISKDFGTRRRLY"
     gene            2003..>2690
                     /gene="tsaD"
                     /locus_tag="VIW27_09725"
     CDS             2003..>2690
                     /gene="tsaD"
                     /locus_tag="VIW27_09725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5739992.1"
                     /translation="MKVLGIESSCDETGIAVYDSDSGEMRQRLYSQIEQHREYGGVVP
                     EIASRDHVLRAAPMVAEIADEIGSISAIDAIAYTRGPGLIGALMVGANLARGLAWALD
                     RPLLGVHHMEGHLLAPMLADDKPELPLVALLVSGGHTLLVDVRAIGDYRILGETLDDA
                     VGEAFDKTAKLLGLPYPGGPELARLADSVASSRFDFPRPMTRRPGLEFSFSGLKTAAL
                     NLVREHQPLDD"
BASE COUNT          451 a          824 c          886 g          529 t
ORIGIN      
        1 accagtacgc cgagacgatc cgggtcgacc gcctggtgaa tgccgtggcc gagattgaag
       61 acgtgcccgg ggtgattgcc gaagcgggta ataatgtcgc tagcctgcgc gcgaatggtc
      121 tgcgggtcgg cgtaaagcac gcagggatcg aggttgccct gcagtgccac ccgatcaccg
      181 atacgctcgc gcgcgttatc gatatccacc gtccagtcga ggccgaccgc gttacagccg
      241 gtcgcggcga tctcctcgag ccattgcccg ccgcccttgg tgaacagggt caccggcacc
      301 ggttgtccgt cctgttcgcg cttgaggccg tcgacgattt gctgcatgta ggccagcgaa
      361 aattcgcggt aagtctgcgg tgacagcaca ccgccccagg tatcgaaaac catcaccgcc
      421 tgcgcgccgg cctcgatctg tgcgttcagg taggtgatca ccgatttcgc cagcgtgtcc
      481 agcaaccgat gcagcgtttg cggatcgccg tacatcattc ccttgatgta ctggtattcc
      541 ttggaactgc cgccctcgac catataggtg gaaagtgtcc acgggctgcc gctgaaaccg
      601 atcagcggta cgcggccgtc gagttcgcgg cgaatcaggc tgaccgcatc gatgacgtag
      661 cgcaggctgt cggcggggtc cggcacgccg agccgcgcga catccgcggc gcggcgcagc
      721 ggacgctcga atttcggtcc ctcgccggtg gcgaagtaga gcccgagccc catcgcctcg
      781 ggtatcgtca ggatatcgga aaacaggatc gcggcatcga ggtcgaagcg cctgagcggc
      841 tgcatggtga cttcgcaggc gagctcggta ttcttgcaca ggtcgaggaa gctgccggcc
      901 tgctcgcggg tagcgcggta ctcgggcagg tagcggccgg cctggcgcat gatccacacc
      961 ggcgtggtgt cggtgggctg tcgcaacagc gcgcgaatca agcgatcgtt tttcagctcg
     1021 ggcagtgaca tcgcgcttcg aactggtcga ggatggccgc gttagccggt cgttgccgat
     1081 cggcgcctcg ggactggccg cgtgcgacgc catgggcgag gtgcgggttc ctgtcggggt
     1141 cggtgttctg tgtttccagc aatcgcaaca gggtcggcat cgggctcggc tgctcggcag
     1201 tgaagctacg cggacagccg cgatttgatc agctggctaa cctggcccat gtccgcgcgc
     1261 ccctgcagtt gcggcttgag catgcccatc accttgccca tgtccctgat cgaggaagcg
     1321 ccggtggcgg cgagggattg ctcgatcagc gcctcgattt cggcctcgga aagggcctcg
     1381 ggcatgaatt caccgatgat ttcgagctcg gcctgctcga tcgcggtgag gtcgtcacgg
     1441 ccggcgcttg cgaactgcga tatcgattcg cggcgttgct tgaccatctt gtcgagcacc
     1501 gcgatgatgc gggtgtcgtc gagttcgatg cgttcgtcga cctccacctg cttgatcgcc
     1561 gacagcatca gccgaatcac gcccagccgc gccttgtcac cgcttttcat cgatgacttc
     1621 atgtcggcct gcagttgtga cttgaggctg tccggcatct tgggtgaatc agtagaggcg
     1681 gcggcgggtg ccgaaatcct tggagatgcg cttgaagttg cgcttgacgg cggccgcgga
     1741 tttgcgcttg cgcacggcgg tcggcttctc gtaatgctgt cggcggcgcg tctcggtgag
     1801 aatgccggct ttctcgcagg aacgcttgaa gcggcgcagt gcaaaatcga atggctcgtt
     1861 gtcctttact ttgactgatg gcatattgat ctggtccagt taaaaaatgt ggtgccgccg
     1921 agcggcccgg tttcaagagc cggccaatta taaggaggca gcaccaaata tcaatcgcta
     1981 tcggcacaaa gccctgtttt atatgaaagt attgggaatc gaaagctcct gtgacgaaac
     2041 cgggatcgcg gtctacgaca gcgacagcgg cgagatgcgc cagcggctgt actcgcagat
     2101 cgaacagcat cgcgaatacg gcggcgtggt gccggaaatc gcgtcgcgcg accacgtgct
     2161 gcgcgccgcg ccgatggtcg ccgaaatcgc cgacgagatc ggttcgatca gcgccatcga
     2221 cgccatcgcc tacactcgcg gtcccggcct gatcggtgcg ttgatggtcg gcgccaacct
     2281 cgcgcgcggc ctggcctggg cgctcgaccg gccgctgctt ggcgtgcacc acatggaagg
     2341 gcatttgctg gcgccgatgc tggccgacga caagcccgag ctgccgctgg tcgcgctgct
     2401 ggtctcgggc ggtcacacgt tgctggtcga cgtgcgcgcg atcggtgact accgcattct
     2461 cggcgaaacc ctcgatgacg cggtcggtga agccttcgac aagaccgcca agctgctcgg
     2521 cttgccgtat cccggcggcc ccgagcttgc gcggcttgcc gactcggtgg cctcgtcgcg
     2581 cttcgatttc ccgcggccga tgacgcgccg cccgggtctc gaattcagtt tcagcggtct
     2641 gaagaccgcg gcgctgaacc tggtgcgcga acatcagccg ctcgacgatg
//
LOCUS       DATCZV010000429         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_353406, whole genome shotgun sequence.
ACCESSION   DATCZV010000429 DATCZV010000000
VERSION     DATCZV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353406"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VIW27_09730"
     CDS             <1..415
                     /locus_tag="VIW27_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY5739993.1"
                     /translation="QVYLVRGSGNAIDLIILPVSGRGYQSTLRAWLVLDGDARTVRAL
                     KFYQHGETPGVGARIEEPEWEAQWRDLSAYDDDGVLRIGVRSHGAGYSDDAQYQVDGI
                     SGATRTAQGVDGMLRFWLGDFGFEPFLQRIREDRL"
     gene            415..1074
                     /locus_tag="VIW27_09735"
     CDS             415..1074
                     /locus_tag="VIW27_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEY5739994.1"
                     /translation="MQESKWRILIRPLIDDNPLTLQILGICSALAVTTSLDTALLMSF
                     VVVCVLALGSGSVSLIRNHLPHSVRIIVQITIIASLVIVVDQVLQAYAFELSKRLSVF
                     VGLIITNCIVLGRAEGYAMHNGFVASVLDGIGNGLGYSLILILVGAIREFFGKGSLLN
                     VQILVPTSQGGAFEPLHLMLLPPSAFFIIGGIIWYIRRKRPQQVEKPEFTLRQLRQES
                     E"
     gene            1071..1676
                     /gene="nqrE"
                     /locus_tag="VIW27_09740"
     CDS             1071..1676
                     /gene="nqrE"
                     /locus_tag="VIW27_09740"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEY5739995.1"
                     /translation="MNALLELLLRSVFLDNLALSFFLGMCTFLAVSKRVDTAFGVGLA
                     VMFVLTVTVPVNNLIYIWLLAPGALDWAGLGDLDLSFLRLIAFIGVIAAMVQVLEMTL
                     EFHVPKLHRAMGIFLPLITVNCAILGGSLFMVERSYSFTESVVFGFGSGLGWALAIVM
                     FAAIRERLKYADIPDGLQGLGMNFIIAGIMSLGFLAFAGMG"
     gene            1676..>2322
                     /locus_tag="VIW27_09745"
     CDS             1676..>2322
                     /locus_tag="VIW27_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5739996.1"
                     /translation="MLEAVLGVVFFSGIVLLLALFVLGARRLLVPRGECDIEINGRKT
                     VRAQIGERLLGVCQQAGIHLPSACAGAGTCGLCKARVVSGGGEAGPQELAHLSQREAA
                     RGGRLACQVPILGPMSIEVDEAYFDIRSWQCTVEQARNVSTLIREIVLRLPEGEPMEF
                     RAGGFVELTSPPYQLEFKDLAIEPEFHDVWDKMNLWRLQAGSEREVTRAYSMANHP"
BASE COUNT          381 a          680 c          742 g          519 t
ORIGIN      
        1 ccaggtttac ctggtgcgcg gcagcggcaa cgcgatcgac ctgatcatac tgccggtctc
       61 ggggcgcggt taccagtcga cgctgcgcgc ctggctggtg ctcgatggtg atgcgagaac
      121 ggtgcgcgcg ctcaagtttt accagcatgg tgaaaccccg ggcgtcggtg ctcgcatcga
      181 ggagcccgag tgggaggccc agtggcgcga tctatcggcc tatgacgatg acggcgtatt
      241 gcgtatcggc gtcaggtcgc atggcgccgg ctacagcgac gacgcgcaat accaggtcga
      301 cggcatttcc ggagccaccc gcaccgccca gggcgtcgac ggcatgctgc gtttctggct
      361 cggcgatttc ggctttgagc catttttaca acgtatacgc gaggaccgac tctgatgcag
      421 gagagtaaat ggcgcattct gatccggccg ctgatcgacg ataatccgct gacgctgcag
      481 attcttggga tatgctcggc actggcggta acgacgtcgc tggatacggc gctgttgatg
      541 agcttcgtcg tggtttgcgt gctcgcgctc ggcagcggct cggttagctt gatccgtaac
      601 cacctgccgc attcggtgcg catcatcgtg caaatcacga tcatcgcgtc gctggtcatc
      661 gttgtcgacc aggtgctgca ggcctacgct ttcgagctca gcaaacggct gtcggtgttc
      721 gtcggtctga ttatcaccaa ctgtatcgta ctcggccgcg ccgagggtta cgcgatgcac
      781 aacggcttcg tcgccagcgt gctcgacggt atcggcaacg ggctcggcta ctcgctgatc
      841 ctgatcctcg tcggagcgat ccgcgagttt ttcggtaagg gcagcttgct caatgtgcag
      901 atcctggtgc cgaccagcca gggtggcgcc ttcgagccgc tgcacctgat gctgttgccg
      961 ccgagcgcat ttttcattat cggcggcatc atctggtaca tacgtcgcaa gcgcccgcag
     1021 caggttgaaa aacccgaatt taccctacgc caactgcggc aggaatccga atgaacgcgc
     1081 tgctcgaact gctgctgcgc tcggtatttc tcgacaatct cgcgctgtcg ttttttctcg
     1141 gcatgtgcac ttttctggcg gtctcgaaac gcgtcgatac cgctttcggc gtcgggctcg
     1201 cggtgatgtt cgtgctaacc gtaacggtgc cggtcaataa cctgatttat atctggctgc
     1261 tggctcccgg cgcgctcgac tgggccggtc tcggcgatct ggatctgtcg tttctgcgcc
     1321 tgatcgcgtt tatcggcgtg atcgcggcga tggtgcaggt gctcgagatg acgctcgagt
     1381 ttcacgtgcc gaaactgcat cgcgcgatgg gtattttcct gccgctgatt acggtgaact
     1441 gcgccattct aggcggtagc ctgttcatgg tcgagcgcag ttatagtttc acggaatcag
     1501 tcgttttcgg tttcggctcg ggcctcggct gggcactggc catcgtcatg tttgccgcga
     1561 tccgggaacg actcaagtat gccgacattc ccgacggcct gcaggggctg gggatgaact
     1621 tcattatcgc cggtatcatg tcgctcggct tcctggcgtt tgcggggatg ggctgatgct
     1681 ggaggccgtt ctcggagtag ttttcttttc cggcatcgtt ctactgctag cgctgttcgt
     1741 gctcggtgca cgacgcctgc tggtgccgcg cggcgagtgc gatatcgaaa tcaacgggcg
     1801 caaaaccgtg cgcgcgcaaa ttggcgaacg cctgcttggc gtttgccagc aagccggcat
     1861 tcatctgccc agtgcctgcg ccggcgccgg tacctgcgga ttatgcaagg cgcgggtggt
     1921 cagcggcggc ggtgaggccg ggccgcagga actcgcccac ctgagccagc gcgaggccgc
     1981 tcgcggcggg cgtcttgcct gccaggtacc gatcctcggc ccgatgtcga tcgaggtcga
     2041 cgaagcctac ttcgatatcc gcagctggca atgcacggtc gagcaggcac gtaacgtcag
     2101 caccttgatc cgcgagatcg tgctgcggct acccgagggc gagccaatgg agtttcgtgc
     2161 cggtggcttt gtcgagctca ccagcccgcc gtaccagcta gagttcaagg atttggcgat
     2221 cgagcccgag tttcatgacg tctgggacaa gatgaacctg tggcggctgc aagcgggtag
     2281 cgagcgcgag gtgacgcgcg cctactcgat ggcgaatcat cc
//
LOCUS       DATCZV010000430         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_512331, whole genome shotgun sequence.
ACCESSION   DATCZV010000430 DATCZV010000000
VERSION     DATCZV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512331"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VIW27_09750"
     CDS             <1..463
                     /locus_tag="VIW27_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5739997.1"
                     /translation="GLISLGLAGGLLTVDSAHPSLAFFGRDWQTPGGIDFMLMIAIGF
                     IAAIGFYCLSRAYCYSEASAIAPFEFTYILWAVAFGYLFWQEVPGPTTIVGVAILVSS
                     SLYIWYRERQVAREETALVPVQAAHLPQQPPDPEIEPLQFAELHAGQHETT"
     gene            complement(575..1375)
                     /locus_tag="VIW27_09755"
     CDS             complement(575..1375)
                     /locus_tag="VIW27_09755"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY5739998.1"
                     /translation="MKRRATILYFPHGGGPLPLLDEPRHAAMNVFLREFSHSLDRPDA
                     IVVVSAHWEETQVSITAAAAPPMIFDFYGFPPEAYEYSYPAPGHPELASRVHRLFGEA
                     GIESRLDTERGFDHGLYVPLMLMYPAADIPCIQISLHAGLDAAQHIRIGRALAALGDE
                     NLLILGSGFSFHNLRELMSNSGDGVDSRNQAFEDWLAQTLTDEGLAESERERRLADWL
                     EAPDARFCHPREEHLLPLHLCYGIAGGPAVCAFREPVLGFLASAYRWD"
     gene            complement(1372..1908)
                     /locus_tag="VIW27_09760"
     CDS             complement(1372..1908)
                     /locus_tag="VIW27_09760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005495801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5739999.1"
                     /translation="MRKRVIAFAASSSRRSINKQLITWACGLLDDAEVEILDLNDYEL
                     PLFSVDREDQLGQPAAARAFLDKIAGGDGIIISFAEHNGAYSAAYKNLFDWVSRIQPK
                     VYRDKPMVLLSTSLGGRGGKSVMELALAQIPRFGGDIRASVSVPSFGDNFDSEAGRVS
                     NPQIAEQIEAAVKRLFAA"
BASE COUNT          365 a          645 c          655 g          355 t
ORIGIN      
        1 cggcctgatc agcctgggcc tcgccggcgg actgctcacc gtcgactcgg cgcacccgtc
       61 gctggccttc ttcggccgcg actggcaaac gcccggcggc atcgacttca tgctgatgat
      121 cgcgatcggc ttcatcgccg caatcggttt ctattgcctg tcacgggctt actgctattc
      181 cgaggcgagc gcgatcgcac cgttcgaatt cacctatatc ctgtgggcgg tggctttcgg
      241 atacctgttc tggcaggaag tgcccgggcc gacgacgatc gtcggcgtcg cgatcctggt
      301 ctccagcagc ctctacatct ggtaccgcga gcgccaggtc gcgcgcgagg aaaccgcgct
      361 ggtaccggtg caggccgcac acctgccgca gcagccgccc gacccggaaa tcgaaccctt
      421 gcagtttgcc gagctgcacg cagggcagca cgagaccacc tgatagcgcg cgggaaggcg
      481 tggtttacgc cgggtaagca aacgttccga acgccgtcag cggatcgatt cccggggacg
      541 cagcgccgcg gccgggccgg atgtcgacgg cccgctagtc ccagcggtag gcgctggcga
      601 ggaaccccag caccggctcc cgaaaggcgc acacggccgg tccaccggcg ataccgtaac
      661 agaggtgcag cggcagcagg tgctcttcgc gcgggtgaca gaagcgtgca tccggggctt
      721 cgagccagtc ggcgagccgc cgctcgcgct cgctttcagc gaggccctcg tcggtcaggg
      781 tttgcgcgag ccagtcctcg aaggcctggt tgcgcgagtc aacgccgtcg ccgctgttcg
      841 acatcagctc tcgcaggttg tgaaacgaga aaccggagcc gagaatcagc aggttttcgt
      901 cgccgagcgc ggccagtgcc cggccgatac gaatgtgctg cgcggcgtcg agaccggcgt
      961 gcagcgagat ctggatacag ggaatatcgg ccgccggata catcagcatc agcggcacgt
     1021 acaggccatg atcgaaaccc cgctcggtat cgagacgcga ctcgatgccg gcttcgccga
     1081 acagccgatg cacgcgcgag gcgagctcgg gatggccggg tgccgggtag ctgtattcgt
     1141 aggcctcggg cggaaaaccg taaaaatcga aaatcatcgg cggcgcagct gctgcggtta
     1201 tcgagacctg ggtttcttcc cagtgcgcac tgacgacgac gatcgcatcg ggcctgtcga
     1261 ggctatgcga aaattcgcgc aaaaagacat tcatcgccgc gtgacgcggt tcgtcgagca
     1321 acggcagcgg accgccgccg tgcggaaaat acaggatcgt cgcgcggcgc ttcacgcggc
     1381 gaacagacgc tttaccgccg cctcgatctg ctccgcgatc tgcggattgc tgacgcgccc
     1441 ggcctcgcta tcgaaattat cgccgaacga cggcaccgag acgctggccc tgatatcgcc
     1501 gccgaaacgc ggaatctgcg cgagcgcgag ttccatgacc gacttgccgc cgcggccgcc
     1561 gagggaggtc gacagcagca ccatcggctt gtcgcggtaa accttcggct gaatgcgcga
     1621 cacccagtcg aagagattct tgtaggcggc gctgtaggcg ccgttgtgct cggcaaagga
     1681 gatgatgatg ccgtcgccgc cggcgatctt gtcgaggaac gcccgcgccg ccgccggctg
     1741 gcccagctgg tcctcgcggt cgacggaaaa cagcggcaat tcgtaatcgt tgagatcgag
     1801 tatttccacc tcggcatcgt cgagcaggcc gcaggcccag gtgatcagct gcttgttgat
     1861 cgagcggcgg ctgctactcg cggcaaaggc gatgactcgt ttccgcatcg attaactctc
     1921 catcaggatc aggttttcgc ggttgatcag ttcgggatcg tcctggtagc cgagaatttc
     1981 cggtatctgc cggctcgact tgccgatgat ctgtttgcac
//
LOCUS       DATCZV010000431         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_141293, whole genome shotgun sequence.
ACCESSION   DATCZV010000431 DATCZV010000000
VERSION     DATCZV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141293"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VIW27_09765"
     CDS             <1..581
                     /locus_tag="VIW27_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5740000.1"
                     /translation="LRFDTTDREATASAIKIIQQQYGDIDILINNVGYRDRRSIQEFA
                     VGDIDRMLANNLVAPFELARLLAPGMMAKGRGRIINVSSVVATFASDGDAAYVAAKGG
                     LESLTRAMAVEYGKGGVTVNAISPGFFLTDPNREMAKNEALNRWLEGRTILGRWAEPE
                     ELAGIAVFLAADESGYITGQTIQVDGGLSARL"
     gene            735..>2352
                     /locus_tag="VIW27_09770"
     CDS             735..>2352
                     /locus_tag="VIW27_09770"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5740001.1"
                     /translation="MTTPSGQRLAAVLMADIAGYTRLVESDTAGTVAAWQACRDRVIE
                     PTVAQFGGQIVKLTGDGFLAEFSSVQRAVDAALEMQRLLAEHPLDFRMGVNFGDIVDD
                     GRDIHGEGVNIAARIEAMAPDGGICITGTVHDAVRNRIATTFQDLGEHPVKHVSNPVR
                     VWQWPAQNLDSVIRGAQERVELPDDTPSIAVLPFAHPSEDSEQADFIDGMTEDLITDL
                     SKISGLFVVARQSSFAYRDRGLSHQAIARELGVRYLLLGKLRRSGSRVRINAQLVDSH
                     NGNEVWAERYDGSLEDVFELQDEVCAQVVEALSVKLSRQESANLRRVHTANLDAYELF
                     VQARSTPYPPIPERIFSARDMFARVIELDPAFAGGYAGVAAMLSFINTWGSDRDSAAI
                     REAIELAEKACSVDDSFGWSHVALGVALLANREHDASIEAILRGIEREPNDAEAHAML
                     SLTLCLAGRYDEAGRSIDQSIRLNPQFVYGPYLNIRGIGQLLYGNCQAAVASFQENEN
                     RQGPVGPPALGLHAAALQELGRQEELELRIE"
BASE COUNT          432 a          765 c          742 g          413 t
ORIGIN      
        1 cgctgcgctt cgacaccacc gaccgcgagg cgaccgcgag cgccataaag atcatccagc
       61 agcaatacgg tgatatcgac atcctgatca acaacgtcgg ttaccgggac cgccgatcga
      121 tacaggaatt cgctgtcggc gatatcgacc gaatgctggc gaataacctg gtcgcaccgt
      181 tcgaactggc gcgattgctc gccccgggga tgatggcaaa gggccgcggg cgaatcatca
      241 acgtttcatc ggtggtggcg acctttgcca gcgacggcga tgcggcctac gtggccgcca
      301 agggcggact ggaatcgctg acccgggcga tggccgtcga atacggcaag ggcggcgtta
      361 ccgtcaacgc gatatccccg ggcttcttcc tcaccgatcc caatcgcgag atggccaaaa
      421 acgaagccct caaccgatgg ctcgaggggc gcacgatcct cggccgctgg gccgagcccg
      481 aggaactcgc cggtatcgcc gtgttcctag ccgccgacga atccggctat atcaccggcc
      541 agaccatcca ggtcgacggc ggcctctccg cccggctgta atccctggaa tgaacctgaa
      601 gtcgggcctt acctttcacg agcagatcga tcgcggtgtg aaatgcgacg cccgaccgtg
      661 tatttttgat ctcctatact gccccgggat ttcgctttag aatccgggca tatgcgcgta
      721 acgactcgca atcgatgaca actccttccg gacaacggct ggccgctgta ttgatggcgg
      781 atatcgccgg ctacacacgc ttggtcgaat ccgacaccgc cggcacggtc gcggcctggc
      841 aggcctgccg cgatcgcgtc atcgagccga cggtggcgca gttcggcgga cagatcgtga
      901 agctgaccgg cgatggtttc ctggccgagt tttcctcggt gcagcgcgcg gtcgacgcgg
      961 cgctcgaaat gcagcgcttg ctcgccgagc atccgctcga ttttcgcatg ggcgtcaatt
     1021 tcggcgacat cgtcgacgac ggccgcgata tccatggcga gggcgtcaac atcgcggcgc
     1081 gcatcgaggc gatggcaccg gacggcggca tctgcattac cgggaccgtg cacgacgcgg
     1141 tgcgtaatcg catcgcgacc accttccagg acctgggcga gcacccggtc aaacacgtat
     1201 ccaacccggt gcgcgtctgg caatggccgg cgcagaacct cgacagcgtc atccgcggcg
     1261 cgcaggagcg cgtcgaactg cccgacgata cgccgtcgat cgcggtattg ccgtttgccc
     1321 atccgagtga agacagcgag caggccgact ttatcgacgg catgaccgag gacctgatca
     1381 ccgacctgtc gaaaatatcc gggctgttcg tggtcgcgcg gcagtcttct ttcgcctacc
     1441 gcgaccgcgg tctcagccac caggccatcg cgcgcgaact cggcgtgcgc tacctgttgc
     1501 tgggcaagct gcgccgctcg ggctcgcgcg tgcgtatcaa tgcgcagctg gttgactcgc
     1561 acaacggtaa tgaagtctgg gcggagcgtt atgacggctc gctcgaggat gtattcgagc
     1621 tgcaggacga ggtttgcgcg caggtggtcg aggcattgtc ggtaaagctc agccggcagg
     1681 aatcggccaa cctgcgccgc gtgcataccg cgaacctgga tgcctacgaa ctgttcgtgc
     1741 aggcccgctc cacgccctac ccgccgatcc cggaacgcat cttctccgcg cgcgacatgt
     1801 tcgcccgggt gatcgaactc gatcccgcct tcgccggcgg ctatgccggc gtcgcggcga
     1861 tgctgagttt tatcaatacc tggggcagcg accgcgatag cgcagcgatc cgcgaggcca
     1921 tcgagcttgc cgagaaggcc tgcagcgtcg acgacagctt cggctggagt cacgtcgcgc
     1981 tcggcgtcgc gttgctcgcc aaccgcgagc acgacgccag tatcgaggcg attctgcgcg
     2041 gcatcgagcg cgagcccaac gacgccgagg cgcatgccat gctcagcctg accctgtgcc
     2101 tggctggccg ttacgacgag gccgggcgat ccatcgacca gtcgatccgg ctgaatccgc
     2161 agtttgtcta tggaccgtat ctcaatatcc gtggcatcgg ccagctgctg tacggcaatt
     2221 gccaggccgc ggtcgcgagt ttccaggaaa acgagaaccg ccaggggccg gtcggcccgc
     2281 cggccctcgg tttgcacgcg gccgcgcttc aggaactggg acgccaggag gaactggaac
     2341 tgcgtatcga gc
//
LOCUS       DATCZV010000432         6437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_671269, whole genome shotgun sequence.
ACCESSION   DATCZV010000432 DATCZV010000000
VERSION     DATCZV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671269"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VIW27_09775"
     CDS             complement(<1..689)
                     /locus_tag="VIW27_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5740002.1"
                     /translation="MPDLQVIEFDRNGPADTGLQPWDPLPAEILLSGNPLQTGHTYHE
                     IDDGVFTAGVWDCTEHQLLASPYEVDEFMIVLEGSIIIEHDDGETRRFREGECFVIPR
                     GTPCSWKQDEYVRKFWAIHAPSTQLAGDPALHAILADPHVPLPPVAGLEASDFETGIP
                     EMGLFSLYRDPTGSFEAGIWECSPMRRVPGRLARSELMHILEGNGAITNADGVVFEFG
                     PGDTFLVPLGMG"
     gene            816..1682
                     /locus_tag="VIW27_09780"
     CDS             816..1682
                     /locus_tag="VIW27_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5740003.1"
                     /translation="MKIVSYNIQFGRGLDGVIDLVRVCAAIRGADIACLQEVDQWWQR
                     SGDCDQAAEIGGLLPTYYYAFGSSFDVDASSRAAHSAVVNRRRRHGNLILSRWPILSM
                     RCLHLPKWHYEDRFNMQMSCVEAVVDAGGHALRIYNCHAGYLDAGERQMQFDYLARAI
                     AAAPGERGAWSGRADIDDDDWSNGRQAPPMPAGALVCGDFNATPDSAELRQLLTATGL
                     VDCWSLADTENRESSTLRKGTSRDIDIAGKVDHILVTQELAGSVEAAAIDQQADGSDH
                     KPVTAVLALPSE"
     gene            complement(1674..2951)
                     /locus_tag="VIW27_09785"
     CDS             complement(1674..2951)
                     /locus_tag="VIW27_09785"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEY5740004.1"
                     /translation="MQFFQELMPSLRLARWAPAVLRKHLSGGESEPIESLCAMMMASD
                     GEYSSLLLAERILNAFERLDDESRLNFFTLLMTDYDVDIDAIKATADDYARYDDAKSL
                     LRLTAAAEPRRQELLRRINLTPGGTRRLVKMREHLMNAMREQPELKKIDTDFLHLFNA
                     WFNRGFLLMQPIDWTTPAHILEKIIAYEAVHEIESWSELRRRLQPSDRYCYGFFHPSM
                     EDEPLVFVEVALTDEIPRGIGEILQPDPDGEVPDNPSCAIFYSISNCHAGLGGVSFGN
                     FLIKQVATSLKLRFPQLKTFCTISPAPGFRRWLENEARDDAELESLVEDFDTDADDAQ
                     RERMARAAARYYLTAKKKTGEPVDPVARFHLKNGAMLERINVLGNPSDKGMESAYGLM
                     VNYVYDLSRVEENHEKYVQNNEVICSSQVRKLL"
     gene            complement(3108..4019)
                     /locus_tag="VIW27_09790"
     CDS             complement(3108..4019)
                     /locus_tag="VIW27_09790"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5740005.1"
                     /translation="MEKTTHLGSAGGSPSLLRRLGRKKDRLLGLLLPGWADSKIENLL
                     FVHNAKSARPVRIPRGFRRLDIMSGQGSIRAYETGNGPTVVFVHGWGGGANQFFPLMH
                     GLAQCGFRSIAFDQLGHGSSEPKPGTLQQTIATTNQVLDKVRRSRDGLCAIVGHSTGC
                     IAIAAARNALVRDVPLFLISPVFNYKLFFLKKLVKLQLHPVVLKKYANGFARAYRGEF
                     RKLELARNLERYGDCTVIAHEESDSESTIADSIAFCDRHPLTRLLVTRHCDHLRIINS
                     ETVWQQLKSHLNYDDTSINFTAEVIYD"
     gene            complement(4147..5145)
                     /locus_tag="VIW27_09795"
     CDS             complement(4147..5145)
                     /locus_tag="VIW27_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY5740006.1"
                     /translation="MSNPVLVNVLRGSHVESRHRGSIAVCDPRGRLVASWGDVENPVY
                     PRSAIKLLQALPLIESGAADHFDLGGAQLALACSSHSAESIHTETVSAWLRNIGLGEQ
                     DLECGPHPPMNETAAAALIRGGIEPGRVHNNCSGKHTGMLTTCRFLDEPTHGYIEPEH
                     PAQQRWFDTLGEMSGADLRRLPADRDGCGIPVVAMPLRAIATAFARVAAPDDLAPARG
                     AAIDRLGVAIADNPPMIAGSGRLCSELIALTGQRLLIKSGAQGIYTASLAEQGLGIAL
                     KIDDGSGEAAEVALLDLLNRLGALHSDELEALAARRRMPITNTVGRITGYRQGVLP"
     gene            complement(5262..6110)
                     /locus_tag="VIW27_09800"
     CDS             complement(5262..6110)
                     /locus_tag="VIW27_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5740007.1"
                     /translation="MDGSFRIVDDACREAFDRHGAVCLRGVFADWIETLRAGVERNLA
                     EPGPYFVDNVAAGGGGRFWDDYCNWQRIPEFRRMITQSGAARLAAEVMGSGSAQFFHE
                     HVLVKEPHTVKPTPWHQDAPYYFVDGNHTVSFWLPLDSVSAAATLRLVAGSHRWPKPV
                     LPVKWLDNEDFYAAERERYMSLPDLDATDSDTTILEWEMQPGDAVLFHFRTVHGARGN
                     LQACRRRAFSMRWVGDDAHYVERPGRTSPPFPGHGMSDRQKLREDWFPVLWPRTAAGE
                     EDHRYR"
     gene            complement(6115..>6437)
                     /locus_tag="VIW27_09805"
     CDS             complement(6115..>6437)
                     /locus_tag="VIW27_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5740008.1"
                     /translation="LASWLFEDGQLAQIESAPAGAWGAVIYLGLVMTALAYALWYRLL
                     GLYNVNQVMPFLLLLPVTSVIGGIVFLGESLTPKIALGGALAICGVALITIQRFGPLL
                     RKPA"
BASE COUNT         1175 a         1943 c         2088 g         1231 t
ORIGIN      
        1 cccatgccca gcggtaccag gaaggtgtcg ccggggccga actcgaatac cacgccgtcg
       61 gcgttggtga tcgcgccgtt accttcgaga atatgcatca gttcggagcg cgcgagcctg
      121 cccggcacgc gtcgcatcgg gctgcattcc cagattccgg cctcgaagct gccggtcgga
      181 tcgcggtata gcgagaacag gcccatttcc ggaataccgg tttcaaagtc ggaggcttcg
      241 agacccgcca ccggcggcag cggcacatgt ggatcggcga gtattgcgtg cagcgccgga
      301 tcgccggcga gttgggtact cggggcgtgg atggcccaga acttgcgcac gtactcgtcc
      361 tgcttccaac tgcacggcgt gcccctagga atcacgaaac attcgccctc gcgaaagcgt
      421 cgcgtttcgc cgtcatcgtg ttcgatgatg atcgagccct cgagcacgat catgaattca
      481 tcgacctcat agggcgacgc aagcagttga tgctcggtgc aatcccagac gccggcggta
      541 aacacgccgt cgtcgatctc gtggtaggta tggccggttt gcagcgggtt gcccgatagc
      601 aggatttccg ctggcaacgg atcccagggt tgcaggccgg tatcggccgg gccgttgcga
      661 tcgaattcga ttacctgcag gtcgggcatc gtggtgtcct cggggttgtc tattgccgaa
      721 tatatcatga cagggcaacg aaaggactgc cgccgtcacc gttgttcgct agaatcgtgc
      781 gcgggggccg ctgcggtcgc gacgaagaaa tccgcatgaa aattgtcagc tacaacattc
      841 agttcggccg cggtctcgac ggcgtaatcg acctggtcag ggtctgcgca gcgattcgcg
      901 gtgccgatat cgcctgcctg caggaagtcg atcagtggtg gcagcgcagt ggtgattgcg
      961 accaggcggc cgagattggc ggtctgctgc cgacttacta ttacgctttc ggatcgagct
     1021 tcgacgtcga cgccagttcg cgcgccgcgc acagcgcggt ggtcaaccgt cgccggcggc
     1081 acggtaatct gatcctgtcg cgctggccga ttctgtcgat gcgctgcctg cacctgccta
     1141 aatggcacta cgaagaccgc ttcaacatgc aaatgtcctg cgtcgaggca gtggtcgacg
     1201 cgggcggaca tgcgctgcgt atctacaact gccacgcggg ctatctcgac gccggcgaac
     1261 ggcagatgca gttcgattat ctggcgcggg cgatcgccgc cgcaccgggc gagcgcggcg
     1321 cctggtcggg ccgcgccgat atcgacgacg acgactggag caacggccga caggcaccgc
     1381 cgatgccggc cggcgcgctg gtttgcggcg atttcaatgc gacgccggac agcgccgagc
     1441 taaggcaatt gctgacagct accggcctgg tggattgctg gtcgctggcc gataccgaaa
     1501 accgggaaag ctcgacgctc agaaagggca ccagccgcga catcgatatc gccggcaagg
     1561 tcgatcatat cctggtgacg caggagctcg caggctcggt cgaagcggcg gccatcgatc
     1621 agcaggccga cggctccgac cataaaccgg ttacggcggt gctggctttg ccgtcagagt
     1681 agctttctga cctgcgacga gcagatgacc tcgttgttct gcacgtattt ttcgtggttt
     1741 tcctcgacgc gcgacaagtc gtagacgtag ttcaccatca gcccgtaggc gctctccatg
     1801 cccttgtccg acggattgcc gagcacgttg atgcgctcca gcatggcgcc gtttttgaga
     1861 tgaaagcgcg ccaccgggtc gacgggttcg ccggttttct ttttcgccgt caagtaatag
     1921 cgcgcggcag cccgtgccat gcgctcgcgc tgggcatcat ccgcgtcggt gtcgaagtct
     1981 tcgaccagcg actcgagttc ggcgtcgtca cgcgcctcgt tttccagcca gcgacgaaaa
     2041 ccgggcgccg gtgaaatggt gcaaaaggtt ttcagctgcg gaaagcgcag tttcaggctg
     2101 gtcgcgacct gcttgatcag gaagttgccg aaggatacgc caccgagacc ggcatggcag
     2161 ttggatatcg agtaaaagat cgcgcagctc ggattgtcgg gtacttcgcc gtcggggtcg
     2221 ggctgcagaa tctcgccgat gccgcgcgga atttcgtcgg tcagcgcgac ttcgacgaat
     2281 accagcggct cgtcttccat cgacgggtga aaaaaaccgt agcagtagcg gtcgctgggc
     2341 tgcagccggc ggcgcaactc ggaccagctc tcgatttcgt gcaccgcctc gtaggcgatg
     2401 atcttttcga ggatatgcgc cggcgtggtc cagtcgattg gctgcatgag caggaagcca
     2461 cggttgaacc aggcgttgaa caggtgcagg aagtcggtgt cgatcttctt cagttccggc
     2521 tgttcgcgca tcgcgttcat caggtgttca cgcattttca ccaggcgtcg cgtgccgccc
     2581 ggggtcaggt tgatgcgccg cagcagttcc tggcgtcggg gctcggccgc cgcggtcagc
     2641 cgcagcaagc tcttggcgtc gtcgtagcgc gcatagtcgt cggcggtcgc cttgatggcg
     2701 tcgatgtcga cgtcgtaatc ggtcatcagc agggtgaaga aattcagccg tgactcgtcg
     2761 tcgagtcgtt cgaaggcatt gagaatgcgt tccgcgagca gcaggctcga gtattcgcca
     2821 tcggaagcca tcatcattgc gcacaaggat tcgatcggct cgctttctcc gccgctgagg
     2881 tgcttgcgca acacggccgg cgcccagcgg gcgagacgca gagaaggcat gagttcctgg
     2941 aagaattgca tcgtaaatcc ggtcgctgaa tcgaatagag tctagtctac cgacgattaa
     3001 ttaaatttga cgatattggc aataattcgc gcccgacagg caggatttcg ggacgcatct
     3061 aacagattca aggaataccg cgatcgcttg caccaaaagt gtgcatttta gtcgtagatg
     3121 acttccgcgg tgaaattaat cgacgtgtcg tcgtagttaa gatgcgactt gagctgctgc
     3181 cagacggtct cggaattgat gatgcgcaga tgatcgcaat gtcgggtcac cagcagccgg
     3241 gtcaacgggt ggcggtcgca aaacgcgatc gaatcggcga tcgtgctttc actgtccgat
     3301 tcctcgtgcg cgatcaccgt gcagtcgccg tagcgctcga ggttgcgcgc cagttccagc
     3361 ttgcgaaatt ccccgcggta ggcgcgggcg aagccgttgg cgtatttctt cagtaccacg
     3421 gggtgcagtt gcagcttgac cagttttttg aggaagaaca gcttgtagtt gaacaccggt
     3481 gatatcagga acagcggcac gtcgcgtacc agtgcattgc gcgcggcggc aatggcgata
     3541 caacccgtcg aatggccgac gatcgcacac aggccgtcgc ggctgcgccg gaccttgtcg
     3601 agcacctggt tggtggtggc gatggtctgt tgcagcgtgc cgggtttggg ttcgctgctg
     3661 ccgtgcccca gctggtcgaa ggcgatcgat cggaagccgc actgtgcgag cccgtgcatc
     3721 agcggaaaga actggttggc gccgccaccc cagccgtgca cgaaaaccac cgtcgggccg
     3781 ttgccggttt cgtaggccct gatgctgccc tgtccgctca tgatgtccag gcggcgaaaa
     3841 ccgcgcggta tgcgtaccgg tctggcggat tttgcattgt gcacgaacaa caggttttcg
     3901 atcttgctgt cggcccagcc cggcagcagt aaaccgagca ggcggtcttt ttttctgccg
     3961 agccgccgga gcaggctcgg cgacccgcca gcggatccca ggtgagtagt tttctccatt
     4021 tggcttacct ctttattatt ctatcgagtc tagccgaagc cgcgacgacg gctgccgccg
     4081 gcacggtaaa tatttcactg caaaaacgac aactatgtgg gcttgacggc ccgtcgtcgg
     4141 ggtgactcag ggcagcacgc cctggcggta gccggttatt cggccgacgg tattggtgat
     4201 cggcatgcgc cgccgcgcgg ccagcgcctc gagttcgtcg ctgtgcagcg cgccgagccg
     4261 gttgagcagg tcgagcagcg cgacctccgc ggcttcaccg ctgccgtcgt cgattttcaa
     4321 cgcgatgccg agaccctgtt cagcgagcga tgcagtgtaa atgccctggg caccggactt
     4381 gatcagcagg cgctggccgg tcagtgcgat cagctcgctg caaaggcggc cactaccggc
     4441 gatcatcggc ggattgtcgg cgatcgccac gccgagcctg tcgattgccg cgccgcgcgc
     4501 gggcgcaaga tcgtcgggtg ccgcaacccg tgcaaaggcg gtggcgatcg cccgtagcgg
     4561 catcgccact accggtatgc cgcagccgtc acggtccgcc ggcagccgcc gcaggtcggc
     4621 tccgctcatt tcgccgagtg tgtcgaacca gcgctgctgt gccggatgtt cgggctcgat
     4681 atagccgtga gtcggttcgt cgaggaagcg gcaagtggtc agcataccgg tatgcttgcc
     4741 cgagcaatta ttgtgtaccc gcccgggctc gatgccgccg cgaatcagcg cggcggcggc
     4801 ggtttcgttc atcggcggat gcggcccgca ctcgaggtcc tgttcaccga gtccgatgtt
     4861 ccgcagccag gcgctgaccg tttcggtatg tattgattca gcgctgtgcg aagaacaggc
     4921 gagcgccagt tgtgcaccgc cgaggtcgaa atgatcggcg gcaccggatt cgatcagcgg
     4981 cagggcctgc agcagcttta tcgcggagcg tgggtatacc gggttttcga cgtcgcccca
     5041 ggatgcgacc agccggccgc gtgggtcgca gaccgcgatc gaaccgcgat gccggctttc
     5101 gacgtgcgag ccgcgcagca cattgacgag gacggggttg ctcatagcgg attcctgctc
     5161 ggcccggtaa agtatcgccg ctatcttaag tcagcgatca ggcccggcaa acatttttcg
     5221 cacgtcgccg cggcgagtga caccgccggc cgaagcggcc tttagcggta gcgatgatcc
     5281 tcctcgccag ccgcggtgcg cggccacagt accgggaacc agtcctcgcg taatttctgc
     5341 ctgtcactca tgccgtggcc cggaaacggc ggcgaggtgc ggccagggcg ttcgacgtag
     5401 tgcgcgtcgt cgccgaccca gcgcatcgaa aacgcccgcc ggcggcacgc ctgcaaattg
     5461 cctcgagcgc cgtgcacggt gcgaaaatgg aacagcaccg cgtcaccggg ctgcatttcc
     5521 cactcgagga tggtggtgtc cgaatccgtg gcgtcgaggt ccggcagcga catgtagcgt
     5581 tcgcgctcgg cggcataaaa atcctcgttg tcgagccact tgaccggcag caccggcttc
     5641 ggccagcggt gcgaaccggc caccaggcgc agggttgcgg cagcggagac cgaatccagc
     5701 ggcagccaga agctgaccgt gtgattgcca tcgacgaagt aatagggcgc gtcctggtgc
     5761 cagggcgtcg gtttgacggt atgcggttcc ttgaccagca cgtgctcgtg aaagaattgc
     5821 gcgctgccgg agcccatcac ttcggcggcc aggcgcgcgg cgccggattg ggtgatcatg
     5881 cgccgaaatt cgggaatccg ctgccagttg cagtagtcgt cccagaaacg gccgccgccg
     5941 cccgcggcga cattgtcgac gaagtagggc ccgggctcgg cgagattgcg ctcgacgccg
     6001 gcgcgcagcg tctcgatcca gtcggcgaac accccgcgca ggcagaccgc gccgtgacgg
     6061 tcgaaggctt cacggcaggc gtcgtcgacg attcgaaaac tgccgtccat cgcctcaggc
     6121 gggcttgcgc agcagcggtc cgaatcgctg aatcgtgatt aacgccacgc cacaaatcgc
     6181 gagcgcgccg ccgagcgcga ttttcggcgt cagcgattcg ccgagaaaga cgatgccgcc
     6241 gatcaccgag gtcaccggca gcaacagcag gaagggcatc acctggttga cgttgtaaag
     6301 cccgagcagg cggtaccaaa gcgcataggc gagagcggtc attaccagcc cgagatagat
     6361 taccgcgccc caggcgcccg ccggagcgct ctcgatctgc gccagctgac cgtcttcgaa
     6421 cagccacgat gccaggt
//
LOCUS       DATCZV010000433         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_566886, whole genome shotgun sequence.
ACCESSION   DATCZV010000433 DATCZV010000000
VERSION     DATCZV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566886"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1411)
                     /locus_tag="VIW27_09810"
     CDS             complement(2..1411)
                     /locus_tag="VIW27_09810"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY5740009.1"
                     /translation="MAPDGLRSQRSLLQRRKSLTTTLQALIDGLIVLLTVYYALYNVQ
                     GFVSTLDAVFMITLLAIMGVTYDQMGVYRQFGGLIGAARKLFFAWSASFAIACLIFIM
                     AQYFDQLSRPALSAVFMIAFAGQLLNRWLLITLQLLNAQAHVDQHNVLLVGGGPLVKH
                     LYDSINGNPWIPEKAIGRIRTRDQTGESELPVPVLGERDDILDVVRAKKVRSVYIAVA
                     LENSHLVEQLYRDLANENIDVHWVPNIFSLDLINHSVKEMAGLPLLTLSESPLIGNHL
                     LFKAIEDKLLASIALLLLSPLMLAVAVLIKLDSPGPVIFRQSRTGWNGREFHIWKFRS
                     MRLHREADGELKQATRDDDRITRIGRFIRKTSIDELPQLFNVLSGTMSLVGPRPHAIE
                     HNTDYDQRIRAYMTRHRIKPGITGLAQIHGYRGETDTLEKMKKRVEYDMQYINNWSFW
                     LDIEILIKTIPALLRDEAY"
     gene            complement(1630..>2067)
                     /locus_tag="VIW27_09815"
     CDS             complement(1630..>2067)
                     /locus_tag="VIW27_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5740010.1"
                     /translation="IIHTAGIDPDDSWKQSVAIGVTHPDVESPLPLLVTPMHASLITS
                     DLDYEGARVAVFLSDPNLQQPISIDNLVSVYGLTPSEAQVAISLANGHSIDEIANVSH
                     HSAHTIRSQLKSVFRKTGVSRQSELIKLLLTGPFAQRRRSRKN"
BASE COUNT          378 a          605 c          681 g          403 t
ORIGIN      
        1 gttaatacgc ttcgtcgcgc aatagcgccg gaatcgtctt gatcaggatc tcgatatcga
       61 gccagaacga ccagttattg atgtactgca tgtcgtattc gacccgtttt ttcatcttct
      121 ccagggtgtc ggtttcaccc cggtagccgt gaatttgcgc cagcccggtg atgcccggct
      181 tgatgcgatg gcgcgtcatg taggcgcgaa tccgctggtc gtagtcggta ttgtgctcga
      241 tcgcgtgcgg ccgcggcccg accagggaca tggtgccgga gagtacgttg aacagctgcg
      301 gcagctcgtc gatgctggtt ttgcgtatga agcggccgat gcgcgtgatg cggtcgtcgt
      361 cgcgggtcgc ctgtttgagc tcgccgtcgg cttcgcgatg cagtctcatg ctgcggaact
      421 tccagatatg aaattcgcgg ccgttccagc cggtgcggct ctggcgaaag atcaccgggc
      481 cgggcgagtc gagcttgatc agcaccgcga ccgccagcat cagcgggctc agcagcaata
      541 gcgcgatcga cgccagcagc ttgtcctcga tggccttgaa cagcaggtgg ttgccgatca
      601 gcggcgattc ggagagcgtc agcagcggca gcccggccat ttccttgacg ctgtggttga
      661 tcaggtcgag cgagaaaata ttgggcaccc agtgaacatc gatgttctcg ttggccaagt
      721 cccgatagag ctgttccacc aggtgcgaat tttcgagcgc gacggcgatg tagacgctgc
      781 gcaccttctt ggcgcgcacc acgtcgagaa tgtcgtcgcg ctcgccgagc accggcaccg
      841 gtagttcgct ttcaccggtt tggtcgcggg tgcggatgcg gccgatggct ttttcgggaa
      901 tccacgggtt gccgttgatg ctgtcgtaga ggtgcttgac cagtggcccg ccgccgacca
      961 gcaaaacgtt gtgctggtcg acgtgcgcct gtgcgttgag cagttgcagc gtgatcagca
     1021 gccagcggtt cagcagttgc ccggcaaacg caatcatgaa taccgccgac agcgccgggc
     1081 gcgacagctg gtcgaaatac tgcgccatga tgaaaatcag gcaggcaatc gcgaaactcg
     1141 ccgaccaggc gaaaaacagc ttgcgcgcgg cgccgatcag gccgccgaac tggcggtaaa
     1201 cgcccatctg gtcgtaggtt acacccatga tcgccagcag cgtgatcatg aacaccgcat
     1261 cgagggtgct gacgaagccc tgcacgttgt acagcgcgta gtagaccgtc agcagcacga
     1321 tcaggccatc gatcagtgcc tgcagcgtcg tggtcagcga tttgcgccgc tgcagcagcg
     1381 agcgctggga gcgaagtccg tcaggcgcca tgatccggca ttccaaaacc tgcgctcgtc
     1441 ccggcgaaag cggggggctg cgaatggaaa aggcttgcag gcgggttcgc gcaagccgtc
     1501 cgcggccggt gcggaatcac cacgccgaag ccggctgccc ggcgggcgaa tacccggcac
     1561 gcaaccgggt cctcgaggcg gccggtttca ttgtcgggat tcatggtgtc gatccggtcg
     1621 gcgccgcggt caattcttcc tcgaccgccg ccgctgcgcg aaaggaccag tcagcaacag
     1681 cttgatcagc tcgctctggc gcgagacgcc ggtcttgcga aagaccgatt tcagttgcga
     1741 gcgaatcgta tgcgcgctgt gatgcgagac gttggcgatt tcgtcgatac tgtggccgtt
     1801 ggccaggctg atcgcgacct gcgcctcgga aggcgtcagg ccgtaaacgc tgaccaggtt
     1861 gtcgatcgag atcggctgct gcaggttggg atcgctcagg aacaccgcga cccgggcgcc
     1921 ctcgtaatcg aggtcggaag tgatcaggct ggcatgcatc ggcgtcacca gcagcggcag
     1981 cggcgactcg acgtccggat gggtgacgcc gatcgcgacg ctttgcttcc acgaatcgtc
     2041 ggggtcgatg ccggcggtgt gaatgat
//
LOCUS       DATCZV010000434         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_671338, whole genome shotgun sequence.
ACCESSION   DATCZV010000434 DATCZV010000000
VERSION     DATCZV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671338"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="argB"
                     /locus_tag="VIW27_09820"
     CDS             complement(<1..596)
                     /gene="argB"
                     /locus_tag="VIW27_09820"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5740011.1"
                     /translation="MTIKKSSIAEILIEALPYIQVLDRKTVVIKFGGNAMVDEELKSC
                     FAQDIVLLKQVGVNPVIVHGGGPQIGKLLEQIGKQSRFIEGMRVTDNETMDVVEMVLG
                     GQVNKQIVSLINRHGGRAVGLTGKDGGMIRARKMRLAGDDLPANDLGQVGEVESIDPA
                     VVVMLDDDDFIPVIAPIGVGADGSAYNINADLVAGELAR"
     gene            complement(612..1817)
                     /gene="coaBC"
                     /locus_tag="VIW27_09825"
     CDS             complement(612..1817)
                     /gene="coaBC"
                     /locus_tag="VIW27_09825"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY5740012.1"
                     /translation="MSLLTDKNILLGITGGIAAYKSAELVRLLIKAGAEVRVVMTPAA
                     REFVQPLTYQALSGHRVYVDLFDAEADSAMDHIELARWCDLLLVAPASADFLGKLNAG
                     YADNLLLTLCLASKRAIAVAPAMNQQMFANPATADNLRQLAARGVFVWGPDEGDQACG
                     DVGPGRMLEPGQLCALIEARFAPGRLDGVELLLTAGPTREAIDPVRYISNRSSGKMGY
                     ALAAAALEQGARVTLVSGPVALPAPAGVERIDVESAEEMYDAVMKRASQADIFIACAA
                     VADYRVDRAAAQKIKKSNASMTLELVPNPDILAAVAALDERPFCVGFAAETSDLEKYA
                     LDKLERKKLDMIAANKVGEAGSGFDADSNQLEVFWHDGSESLARAGKAEIAKRLIELV
                     AERYTAARR"
     gene            complement(1828..>2106)
                     /locus_tag="VIW27_09830"
     CDS             complement(1828..>2106)
                     /locus_tag="VIW27_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740013.1"
                     /translation="CRRRSIAHSSRFRCCRPDAGKCPAVTGMPLVGAGPVSFKSRTSR
                     QWRLTISTGVSQHRVGPGSGGKWRAVYIFAAGSAPVSAMLMQYAGYPR"
BASE COUNT          368 a          722 c          638 g          378 t
ORIGIN      
        1 cgcgccagct cgccagcaac caggtcggca ttgatattgt aggccgagcc gtccgcaccg
       61 actccgatcg gcgcgatcac cggaatgaaa tcgtcgtcgt cgagcatcac caccaccgcc
      121 gggtcgatcg attcgacctc gcccacctgg ccgagatcat tggccggcag atcatcgccg
      181 gcgaggcgca tcttgcgcgc gcgaatcatg ccgccatcct tgccggtcag acccaccgcg
      241 cggccgccat gacggttgat cagtgacacg atctgcttgt tgacctgacc gccgagtacc
      301 atttcgacga catccatggt ttcgttatcg gtgacgcgca tgccctcgat gaagcggctt
      361 tgcttgccga tctgctcgag cagcttgccg atctgcggcc cgccgccgtg cacgattacc
      421 gggttgacgc cgacctgctt gagcagcacg atgtcctgcg caaagcagct cttcagctcc
      481 tcgtcgacca tcgcgttgcc gccgaacttg atgaccacgg tcttgcggtc cagtacctgg
      541 atataaggca gggcctcgat cagaatctcg gcgatgctgc tttttttgat cgtcatcggt
      601 gctccagcgg gttatcgacg cgccgcagta tagcgctcgg cgaccagctc gataagccgt
      661 ttggcgattt ccgccttgcc ggcccgcgcc aacgactcgc tgccgtcgtg ccagaaaacc
      721 tccagctgat tgctgtcggc gtcgaaaccg ctgccggctt cgccaacctt gttggcggcg
      781 atcatgtcga gtttcttgcg ctcgagcttg tcgagcgcgt atttctcgag gtcgctggtc
      841 tcggccgcga aaccgacgca aaacgggcgc tcgtcgagcg ccgcgaccgc cgccaggata
      901 tccgggttcg gcaccagttc gagcgtcatc gatgcgttcg atttcttgat cttctgcgcc
      961 gcggcccgat cgacgcggta atccgcgacc gcggcacagg cgatgaagat atcggcctgc
     1021 gaagccctct tcatgaccgc atcgtacatt tcctcggccg actcgacgtc gattcgttcg
     1081 acgccggcgg gtgccggcag cgctaccggc ccgctgacca gtgtcacccg cgcaccctgc
     1141 tcgagtgctg cggcggcaag cgcatagccc atcttgccgg aactgcgatt gctgatatag
     1201 cgtaccgggt cgatcgcctc gcgggtcggg ccggcggtca gcaacagctc gacgccgtcg
     1261 agccggccgg gtgcaaaccg cgcctcgatc aatgcgcaca gctggcccgg ctcgagcatg
     1321 cggccggggc cgacgtcgcc gcaggcctgg tcaccctcgt ccgggcccca gacgaacacg
     1381 ccgcgcgccg ccaactggcg caggttgtcg gcggtcgccg ggttggcgaa catctgctga
     1441 ttcatcgccg gcgccaccgc gatcgctcgc ttgctggcca ggcacagggt cagcaacagg
     1501 ttatcggcat agccggcatt gagcttgccg aggaaatcgg ccgacgccgg cgccaccagc
     1561 agcaggtcgc accagcgcgc cagctcgata tgatccatcg ccgaatcggc ctcggcatcg
     1621 aacaggtcga cataaacgcg gtgcccggag agcgcctggt aggtcagcgg ttgcacgaac
     1681 tcgcgcgccg ccggcgtcat caccacgcgc acctcggcgc cggccttgat caacaggcgt
     1741 accagctcgg cggacttgta agcggcaatg ccgccggtaa tcccgagcag gatattcttg
     1801 tcggtgagca gactcatgcc ggctattcta gcgcggatag cccgcatatt gcatgagcat
     1861 tgcggatacc ggcgcggaac cggcggcaaa gatataaacg gcgcgccatt taccgcccga
     1921 acccggtcca acgcgatgct gcgatacgcc tgtggaaatc gtcaaccgcc attgacgact
     1981 cgttcggctc ttgaacgaca cgggcccggc gcccaccagc ggcattccgg tcaccgccgg
     2041 gcatttcccg gcatcaggcc gacagcaccg gaaccgactc gaatgcgcga tcgagcggcg
     2101 ccgaca
//
LOCUS       DATCZV010000435         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_724300, whole genome shotgun sequence.
ACCESSION   DATCZV010000435 DATCZV010000000
VERSION     DATCZV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724300"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VIW27_09835"
     CDS             <1..1635
                     /locus_tag="VIW27_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011047381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740014.1"
                     /translation="RDLRGGNVAYLAQSAAATFNPAISINEQVTEAAVLHGRLNQQQA
                     DQRAIELYHALELPDADRIGRRYPHQVSGGQLQRLMAAMALCGKPDLLVLDEPTTALD
                     VTTQIEVLKAFKKVIQEENSAAIYVTHDLAVVAQVADHIVVLYGGEVMEQGPADQIIN
                     RPQHAYTRRLMEAVRPKPAAGQGVAISGEHDRVVANLEVVNLTAGYGGIVDGEPAIPI
                     LKDINLRVMNGHVVGVIGESGCGKSTLARVMAGMLPQARGDIILDGEKLEGSLKDRKL
                     EQLRKVQFVFQMADTALNPKQLIGNIIGRPLEFYHGLSGKKKHARVSEILELVELPPA
                     FASRYPMELSGGQKQRINLARSLAAHPEVMLCDEVTSALDSIVGANVIELLTKLRDET
                     GVSFVFISHDLSTVSSFADEIVVLYAGRVVEQGPTDEVLEPPFHPYTRLLISSVPEMR
                     IGWLEDTMKKREMAVGIARGVEITAVGCPFYNRCPLAIDGTCEVKAPPVRELNDGHQI
                     ACHLTIEQLEEAEAEAQRILLGYEKLGEEDAQVHVP"
     gene            1655..2038
                     /locus_tag="VIW27_09840"
     CDS             1655..2038
                     /locus_tag="VIW27_09840"
                     /inference="COORDINATES: protein motif:HMM:NF023370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HEY5740015.1"
                     /translation="MSDYPNLANMGILHPEEISKFAVYTVGNTDILHITYDRKKGSLL
                     AESRRYKFPQIKRTVMVDGGTDKAQSLFESTQAFRDALHELEQLKKVRESGSDQRAAI
                     REEISHLEEEIAWRLEYIRKLTDKL"
     gene            complement(2035..>2387)
                     /locus_tag="VIW27_09845"
     CDS             complement(2035..>2387)
                     /locus_tag="VIW27_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740016.1"
                     /translation="HLEPTQMIAASGALVLVSGLGAVAGPLVFAAIMDFFGNRALFVA
                     IAAVHALTAVFAVYRMAKSAPVPMDRQGRSHATAVHPSGSAIESVQQYWSEETGWNPG
                     EDEGQGGGDSSRGG"
BASE COUNT          507 a          784 c          727 g          369 t
ORIGIN      
        1 cgcgacctgc gcggggggaa cgtcgcgtat ctcgcgcaga gcgcggcggc caccttcaac
       61 ccggcgatca gcatcaacga gcaggtcacc gaggccgcgg tactgcacgg acgcctgaac
      121 cagcaacagg ccgaccagcg cgcgatcgaa ctgtatcatg cgctggaact gcccgatgcc
      181 gatcgcatcg ggcggcgcta cccgcaccag gtgtccggcg gccagctgca gcgcctgatg
      241 gcggcgatgg cgctgtgtgg caaacccgac ctgctggtgc tcgacgaacc gaccacggcg
      301 ctcgacgtaa ccacccagat cgaggtgctg aaggcgttca agaaagtcat ccaggaagaa
      361 aactcggctg cgatctacgt cacccatgac cttgccgtag tcgcccaggt cgccgaccat
      421 atcgtcgtgc tctacggcgg cgaggtcatg gagcagggcc cggccgacca gatcatcaac
      481 cgcccgcagc acgcctacac caggcgcctg atggaagccg tgcggccgaa acccgccgcc
      541 ggccaggggg tcgccatttc cggcgagcac gaccgggtgg tcgccaacct cgaggtcgtc
      601 aacctgactg caggctacgg cggtatcgtc gatggcgaac cggcgatacc gatcctcaag
      661 gatatcaacc tcagggtcat gaacggccac gtggtcggcg tgatcggcga atccggctgc
      721 ggcaaatcga cgctcgcccg agtgatggcc ggcatgctgc cgcaggcgcg cggcgacatc
      781 atcctcgacg gcgagaaact cgagggcagt ctcaaggacc gcaagctcga gcaactgcgg
      841 aaggtgcagt tcgtattcca gatggcggac acggcgctga acccgaaaca attgatcggc
      901 aatatcatcg gccggccgct cgagttctac catggcctca gcggcaagaa aaaacacgcc
      961 cgggtatccg agatcctgga actcgtcgaa ttgcccccgg ctttcgccag ccgctacccg
     1021 atggaactgt ccggcggaca gaagcagcgc atcaacctcg cgcgctcgct ggccgcacat
     1081 cccgaggtca tgctgtgcga cgaggttacc tcggcgctcg acagcatcgt cggcgccaac
     1141 gtcatcgagc tgttgaccaa gctgcgcgac gagaccggcg tttcgttcgt gttcatcagc
     1201 cacgacctgt cgacggtctc ctcgttcgcc gacgaaatcg tcgtgctcta tgccgggcgg
     1261 gtggtcgaac agggtcccac cgacgaagtg ctcgaaccac cgtttcaccc ctatacccgc
     1321 ctgttgatca gctcggtgcc ggagatgcgc atcggctggc tcgaggacac gatgaaaaaa
     1381 cgggaaatgg cggtcggtat cgcgcgcggc gtggaaatca ctgctgtcgg ctgcccgttt
     1441 tacaaccgct gcccgctggc catcgacggc acctgcgaag tcaaggcacc accggtgcgc
     1501 gaactcaacg acggccacca gatcgcctgt cacctgacca tcgagcaact cgaagaagcc
     1561 gaggccgagg cgcagagaat tctcctcggt tacgagaaac tgggcgaaga ggatgcccag
     1621 gtacacgttc cctgacagca cgggtaacag accaatgagc gactatccga atctggcaaa
     1681 catggggatc ctgcatcccg aggaaatcag caaatttgcc gtctacaccg tcggcaatac
     1741 cgatatcctg catatcacct acgatcgcaa gaagggttcg ctgcttgccg aaagccggcg
     1801 ctacaagttc ccgcagatca agagaacggt gatggtcgac ggcggcaccg acaaggcgca
     1861 atcgctgttc gagagtacgc aggcgtttcg cgacgcgctg cacgagctcg agcaactgaa
     1921 aaaagtccgc gaaagcggca gcgatcagcg ggccgcgatc agggaggaga tcagccatct
     1981 cgaagaggaa atcgcctggc ggctcgaata catccggaag ctgaccgaca aactctagcc
     2041 gccgcgactc gagtcgccac caccctgccc ttcgtcttca ccgggattcc agccggtttc
     2101 ctcgctccag tactgctgca ccgattcgat cgccgagccc gacgggtgta ccgcggtggc
     2161 gtgactgcgc ccctgccggt ccatcggcac cggcgcgctt ttcgccatgc gataaaccgc
     2221 gaacacggcg gtcagcgcat gtaccgcggc gatcgcgacg aacagtgcgc ggttgccgaa
     2281 aaaatccatg atcgcggcga acaccagcgg gccggcaacc gcaccgagac cgctgaccag
     2341 caccagcgca ccgctggcgg cgatcatctg cgtcggctcg aggtggt
//
LOCUS       DATCZV010000436         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_248129, whole genome shotgun sequence.
ACCESSION   DATCZV010000436 DATCZV010000000
VERSION     DATCZV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248129"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..508
                     /gene="gcvH"
                     /locus_tag="VIW27_09850"
     CDS             119..508
                     /gene="gcvH"
                     /locus_tag="VIW27_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY5740017.1"
                     /translation="MSEVPSELKFLSSHEWVLVEDNIATIGISDHAQELLGDLVYVEL
                     PEVGSVIAAGDSVGVVESVKAASDTYAPVSGEVVEINEELEGAPERINNDPYGDGWMY
                     KVSLEDPEEVEDLLDAEAYADSIEEED"
     gene            621..983
                     /locus_tag="VIW27_09855"
     CDS             621..983
                     /locus_tag="VIW27_09855"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018951167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY5740018.1"
                     /translation="MAYSGNSGFTRIFKATRYSWQGLRAAYRHEEAFRQETWLCALLV
                     PLGLYLGDGGVEKALLVGSALLLPLVEILNSALEAVVDRIGDEHHELSGRAKDMGSAA
                     VGLAILLTASTWLLVLLS"
     gene            1104..1943
                     /gene="lgt"
                     /locus_tag="VIW27_09860"
     CDS             1104..1943
                     /gene="lgt"
                     /locus_tag="VIW27_09860"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY5740019.1"
                     /translation="MIDYPSIDPVALALGPLKIHWYGVMYLLAFASAWWLGKRRARLD
                     WTPVKPEQVDDLIFYGALGAVLGGRLGSVLFYNFGSFIADPLYLFRIWEGGMSFHGGF
                     LGVVAAMWLFQRKIGSGFFRLTDFIAPLVPLGLAAGRLGNFINAELWGAPTSVPWAVK
                     LPCERFPPQAFVDYAGPLCYAPRHPTQLYEMLFEGLLLFAVLWLLSARPRPLMTASGA
                     FLLLYGLARCGVELIRLPDAHIGYLLGTDWLTRGIVLSLPMIIVGLIFLVLARRHSNA
                     AIP"
     gene            1927..2721
                     /locus_tag="VIW27_09865"
     CDS             1927..2721
                     /locus_tag="VIW27_09865"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY5740020.1"
                     /translation="MRQYLDLMRHVLEQGSEKTDRTGTGTRSVFGYQMRFDLAEGFPL
                     VTTKKLHLHSIIHELLWFLQGDTNIDYLRRNQVRIWDEWADENGDLGPVYGAQWRSWA
                     AADGHTIDQISQLVEQIRDNPDSRRLIVSAWNVGELDKMALPPCHAFFQFYVADGRLS
                     CQLYQRSADVFLGVPFNIASYSLLLMMVAQVTGLEAGDFVHTLGDAHLYSNHLQQALT
                     QLEREPFALPTMRLNPEVDDLFAFRYEDFELVNYRYHEHIKAAVAV"
     gene            2754..3137
                     /gene="apaG"
                     /locus_tag="VIW27_09870"
     CDS             2754..3137
                     /gene="apaG"
                     /locus_tag="VIW27_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY5740021.1"
                     /translation="MNDNPLNQIQVDVETRYVEEQSNPEKNYYVFAYTITIRNKGRQP
                     ARLLTRHWVITDSNQKVQEVRGEGVVGEQPHLKPGEQFVYTSGTMLETAVGTMKGSYQ
                     MQADDGSQFDAEIDEFVLSTPRVLH"
     gene            3150..>3522
                     /locus_tag="VIW27_09875"
     CDS             3150..>3522
                     /locus_tag="VIW27_09875"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY5740022.1"
                     /translation="MAIYAIGDVQGCYDELCRLLDKIGFDPAADTLWFCGDLVNRGPD
                     SLRTLQFVKSLGDSALTVLGNHDLHLLALHHGVKKARGTRDLQKILNSPERDELLAWL
                     QARPLLHYDKRHHALLVHAGIH"
BASE COUNT          642 a         1069 c         1109 g          702 t
ORIGIN      
        1 caggcgtcga cggcatcgtc gaggtcgagt tgcgcaacaa gctgctgcag gcagaaattg
       61 ttaaacttcc cttcgtccgc gagggaaaat ccatgttaac caatggggga aacgtttcat
      121 gagcgaagtg ccatcagaac tgaaattcct gtcttcgcac gaatgggtgc tggtcgaaga
      181 caatatcgcg accatcggaa tttccgatca tgcccaggaa ttactgggtg atctcgtcta
      241 cgtcgagttg cccgaggtcg gcagcgtgat cgccgccggt gattcggtcg gcgtcgtcga
      301 gtcggtcaag gcggcttccg acacctatgc cccggtgagc ggcgaagtcg tcgagatcaa
      361 cgaagaactg gaaggcgcgc ccgagcgaat caacaacgac ccctacgggg acggctggat
      421 gtacaaggtt tcgctggaag atcccgagga agtcgaagac ttgctggacg cggaagccta
      481 cgccgattcc atcgaggaag aagactaggc agccgcgctc gcggaaccgc gcggcgccgc
      541 ctgtcctcgc ggcggccagc ggcgttgcgc gcgagccgcg tttcgccggc ggcaagcgca
      601 ctgcaaggcg gcatttcggc atggcttaca gcggcaacag cggctttacc cgtatcttca
      661 aggcgacgcg ctactcgtgg caggggctgc gggccgcgta tcgccacgag gaagcctttc
      721 gccaggaaac ctggctgtgc gcgctgctgg tgccgctggg cctgtatctc ggcgatggcg
      781 gtgtcgaaaa ggccctgctg gtgggcagtg cactgctgct gccgctggtc gaaatcctca
      841 actcggcact ggaagccgtg gtcgaccgga tcggcgacga gcaccacgag ctctcgggac
      901 gcgccaagga catgggctcg gccgcggtgg ggctggcgat tttgctgacc gccagtacct
      961 ggttgctggt gctgctgtcg taggcgatgg gccgcctgat cgactgccga ggtggtcggt
     1021 tgcgaactgc cctgacgccg ctttccgtgc gataatgtcg cttgctcggg caggctcatg
     1081 gagccgattc caaatatccc gcaatgattg attatccgtc gatagatccg gtcgcgctgg
     1141 cgctcggccc gctgaagatc cactggtatg gggtgatgta cctgctggcg tttgccagcg
     1201 cctggtggct gggcaagcgc cgcgcgcggc tcgactggac cccggtaaag cccgagcagg
     1261 tcgacgacct gatattttac ggcgcgctcg gcgcggtgct cggcggacgg ctcggctcgg
     1321 tactgtttta caacttcggc agttttatcg ccgatccgct gtatctgttt cgcatctggg
     1381 aaggcggcat gtcgtttcac ggcggatttc tcggcgtggt tgcggcgatg tggctgtttc
     1441 agcgcaagat cggcagtggc ttcttcaggc tcacggattt tatcgcgccg ctggtgccgc
     1501 tggggctcgc cgccgggcgt ctcggtaatt ttatcaacgc cgagctgtgg ggcgcgccga
     1561 ccagcgtgcc gtgggcggta aaactgccct gtgagcgctt tccgccgcag gccttcgtcg
     1621 attacgccgg gccgctgtgc tatgccccgc ggcatccgac gcagctttac gaaatgctgt
     1681 tcgagggatt gctgctgttc gccgtgctgt ggctgttgtc ggcgcgaccg cggccgctga
     1741 tgaccgcgtc gggtgccttc ctgctgctct acgggctggc ccgttgcggg gtggaactga
     1801 ttcgcctgcc cgacgcccat atcggctacc tgctgggcac cgactggctg acccggggca
     1861 ttgtattatc gctgccaatg attatagtgg gcctaatttt tctggtcctc gcgagacgac
     1921 attcgaatgc ggcaatacct tgacctgatg cgccacgtgc tcgagcaggg cagcgaaaaa
     1981 accgaccgta ccggcaccgg cacgcgctcg gtattcggct accagatgcg ctttgacctg
     2041 gccgagggtt ttccgctggt gaccaccaag aaactgcacc tgcactcgat catccacgag
     2101 ctgttgtggt ttttgcaggg ggataccaac atcgactacc tgcgccgcaa ccaggtgcgc
     2161 atctgggacg agtgggccga cgaaaacggc gacctcggcc cggtctacgg tgcccagtgg
     2221 cgctcgtggg cggcggccga cggccacacc atcgaccaga tttcgcagct ggtcgagcag
     2281 attcgcgaca acccggattc gcgccgcctg atcgtcagcg cctggaacgt cggcgagctc
     2341 gacaagatgg cgttgccgcc gtgccatgcc ttcttccagt tctacgtcgc cgacgggcgg
     2401 ttgtcgtgcc agctctacca gcgcagcgcg gacgtatttc tcggcgtacc cttcaatatc
     2461 gcctcttatt cgctgttgct gatgatggtc gcccaggtta ccggtctgga ggccggcgat
     2521 ttcgtacata ccctcggtga tgcccacctc tacagcaatc atttgcagca ggcgctgacc
     2581 cagctggaac gcgaaccgtt tgcgctgccg accatgcgcc tcaatcccga ggtcgacgat
     2641 ctgttcgcgt ttcgctacga ggatttcgaa ctcgtcaact accgttacca cgaacacatc
     2701 aaggctgccg tcgctgtata atgcggccac tccaggagtc acgagccgag ccgatgaacg
     2761 ataacccgtt aaaccaaatc caggtcgatg tcgagacccg ctacgtcgag gagcaatcca
     2821 atcctgagaa aaactattac gtgttcgctt atacgatcac catccgcaac aaggggcgcc
     2881 agcccgcacg gctattgacc cgccactggg taataacaga ttcgaatcaa aaggtgcagg
     2941 aggtgcgcgg cgagggcgtg gtcggcgagc aaccgcacct gaaacccggt gagcagttcg
     3001 tctacacctc gggcacgatg ctggaaaccg cggtcggcac gatgaagggc agttaccaga
     3061 tgcaggccga tgacggttcg cagttcgacg ccgagatcga cgaattcgtg ctgtccacgc
     3121 cgcgggtact gcattaacag ccgtcgccgg tggcaatcta cgcgataggc gacgtccagg
     3181 gctgttacga cgagctctgc aggctgctcg acaagatcgg tttcgatccg gcggccgaca
     3241 ccctgtggtt ttgcggcgac ctggtcaacc gcggcccgga ttcgctgcgc acgctgcaat
     3301 tcgtcaaatc cctcggcgat agcgcgctga ccgtgctcgg caaccacgat ctgcacctgc
     3361 tggcgctgca ccatggcgtg aaaaaggccc gtggcacgcg cgacctgcag aaaattctca
     3421 acagccccga gcgtgatgaa ttgctcgcct ggttgcaggc caggccgctg ctgcattacg
     3481 acaagcgcca ccacgcgctg ctggtgcatg cgggaatcca tc
//
LOCUS       DATCZV010000437         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_459703, whole genome shotgun sequence.
ACCESSION   DATCZV010000437 DATCZV010000000
VERSION     DATCZV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459703"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VIW27_09880"
     CDS             <1..1275
                     /locus_tag="VIW27_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740023.1"
                     /translation="MPLMFRAKKILVVGDDRQTVLQKESVIDDYLFRDHELDEHLRST
                     QARGFKGGGSHIFALVKAIKQASVLLDEHYRCPADIIEFSNRYVYDGELNVMQWRLPE
                     HEAAVVVDYSENNVDASKKPTSGKFKGIETGMIDRFMDFVVSNLKAIEKTSGRRIHVD
                     TDVALCYFLLKNEPYVKQVKEHYLRKLNRGDNLLDGAGAALQGKERDYVFYFWDITRY
                     NLGAFKQGDDAEKRKGELNVLMSRPKKKAFHFLHRNFEQLEHGRSNISSYLWQAYHRQ
                     QQAGQRTEGNALDESLLGSLLNFTLSTSGNRGLKDVRRNVAESLLEFRENIVVGDAGR
                     QVDLIAYANGDAREVVGVVDLSAFGCEAGVGRSVVDYYFQLKRVSPGIDPVFVMPHEL
                     IEENGQTFRALMQKLELLDVGRGKRSLKLKKA"
     gene            complement(1435..>2680)
                     /locus_tag="VIW27_09885"
     CDS             complement(1435..>2680)
                     /locus_tag="VIW27_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY5740024.1"
                     /translation="YAGHQFGSWNPRLGDGRAILLGEVIGADGERYDLQLKGSGITPF
                     SRMGDGRAPLGPIVREYLVSEAMAALGVPTCRTLAAVTSGEAVYRESRLDGGVLVRVA
                     KSHIRIGTLQYFASIEDLDGLRLLVEHVIARHYPEAAAADNPVLAMYEQVLQRQAQLI
                     GHWQTLGFIHGVMNTDNMLLSGETIDYGPCAFIDGYDSSAVFSSIDHGGRYAFRNQPG
                     IAHWNLAVLAQTLLPLFADDDERAAEIAQTALDTFPERYAEAYLERLRNKFGLTRVEA
                     DDVALMQEFLDLLERGRSDFTLAFRRLADLAADAPGVESLFDFPDHFDDWLARWRERC
                     RRETGDAAARGRQMAAANPAFIPRNHLVEDAITSAYDGDFTPFHRLLERLQDPYSYAG
                     DDADLASPPRPEQIVQQTFCGT"
BASE COUNT          528 a          805 c          857 g          490 t
ORIGIN      
        1 atgccactga tgtttcgcgc caagaagatc ctcgtcgtcg gtgatgaccg gcagaccgtg
       61 ctgcagaagg aatccgtgat cgacgattac ctgttccgcg accacgagct cgacgaacac
      121 ctgcggtcga cgcaggcgcg cggcttcaag ggcggcggct cgcacatttt cgcgctggtc
      181 aaggcgatca agcaggcatc ggttttgctc gacgagcatt accgctgtcc cgccgatatc
      241 atcgagtttt ccaaccgcta cgtctacgac ggcgagttga acgtcatgca atggcgcctg
      301 cccgaacacg aggccgcggt ggtcgtcgat tacagcgaaa acaatgtcga cgcatcgaag
      361 aagccgacca gcggcaagtt caagggcatc gagaccggta tgatcgatcg cttcatggat
      421 ttcgtggtca gtaacctgaa agccatcgaa aagaccagtg gcaggagaat ccatgtcgat
      481 accgatgtcg cgctgtgtta tttcctgctc aagaacgaac cctacgtcaa gcaggtcaag
      541 gaacactatc tgcgcaaact caatcgcggc gacaatctgc tcgacggtgc gggtgccgcg
      601 ctacagggca aggagcgcga ttatgtgttc tacttctggg atatcacccg ctacaacctc
      661 ggtgccttca aacagggcga cgatgccgaa aagcgcaagg gcgagctgaa cgtattgatg
      721 agtcgcccga agaaaaaggc gtttcacttt ctgcaccgca acttcgagca actcgagcac
      781 ggcaggagca acatttcgag ttacctgtgg caggcctacc atcgccagca gcaggcaggg
      841 cagcgcaccg agggtaatgc gctcgacgag tcgctgctcg gcagcctgct caattttacg
      901 ctgtctacct ccggcaaccg cggcctcaag gacgtgcgcc gcaatgttgc cgaatcactg
      961 ctcgagtttc gcgagaatat cgtcgtcggc gatgccggcc ggcaggtcga cctgatcgcc
     1021 tacgccaacg gtgatgcgcg tgaagtcgtt ggcgtggtcg atctgtcggc cttcggctgc
     1081 gaggccggtg tcggccggtc ggtggtcgat tactacttcc agttgaaacg ggtatcgccc
     1141 ggcatcgacc cggtgttcgt gatgccgcac gaactgatcg aggaaaatgg ccagaccttt
     1201 cgcgcgctga tgcagaaact cgaactgctc gacgtcggcc gcggcaagcg gtcgctgaag
     1261 ttgaaaaagg cctgacccca gcccgggcgt ttgcctgcag caagtgatga atcgatcagg
     1321 cgaagatatt cggtaaaact gcgagcgtcc agcagggccg gcgtcgatcc ggcgcttgcc
     1381 ggctggcggc agggctacgc ggtgttcgcc gggttggctc gttgactcca ggcttcaggt
     1441 accgcagaag gtttgctgca cgatctgctc ggggcgcggc ggactggcca ggtcggcgtc
     1501 gtcgccggca taggaatagg ggtcttgcag tctttccagc aaccggtgaa aaggcgtgaa
     1561 gtcaccgtca taggcactcg ttatggcgtc ctcgacgagg tggttgcggg gaataaaagc
     1621 cgggttggcg gccgccattt gccgcccgcg cgccgccgcg tcgccggttt cacgcctgca
     1681 gcgctcgcgc cagcgcgcca gccagtcgtc gaaatggtcg ggaaaatcga acagcgattc
     1741 gacgcccggg gcatcagccg cgaggtcggc gagacggcga aacgcgagcg tgaaatcact
     1801 gcgcccgcgc tccagcaggt cgaggaattc ctgcatcagc gcgacatcat cggcttccac
     1861 ccgggtcagc ccgaatttgt tccgcagtcg ttcgagataa gcttccgcgt agcgctcggg
     1921 aaaggtatcg agggcggttt gcgcgatttc ggccgcgcgc tcgtcgtcgt cggcgaacag
     1981 cggcagcagc gtctgcgcga gaacggccag gttccagtgc gcgatgccgg gctgattacg
     2041 gaatgcatag cggccgccat gatcgatcga gctgaaaacc gccgacgaat cgtagccatc
     2101 gatgaacgcg caggggccgt aatcgatggt ctcgcccgac agcagcatgt tgtcggtatt
     2161 catcacgccg tgaatgaatc ccagggtttg ccagtggccg atcagttgcg cctggcgttg
     2221 cagcacctgc tcgtacattg ccagcaccgg attatcggct gcggcggcct cggggtaatg
     2281 ccgcgcgatg acgtgttcga ccagcaggcg caggccgtcc aggtcttcga tcgaggcgaa
     2341 gtattgcagg gttccgatgc gaatatggct cttcgcgacg cgcaccagca cgccgccgtc
     2401 gagacgcgat tcgcggtaga ccgcctcgcc gctggtgacc gccgccagcg tacggcaggt
     2461 gggcacgccg agcgccgcca tcgcctcgct gaccaggtat tcgcgaacga tcggtccgag
     2521 cggtgcgcga ccgtcgccca tgcgcgaaaa cggtgtaatg ccggagccct tgagctgcag
     2581 gtcgtagcgc tcgccgtcgg cgccgatcac ctcgccgagc aggatcgcgc ggccgtcgcc
     2641 gagacgcgga ttccagctgc cgaactgatg gcccgcatag
//
LOCUS       DATCZV010000438         4111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_567029, whole genome shotgun sequence.
ACCESSION   DATCZV010000438 DATCZV010000000
VERSION     DATCZV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567029"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VIW27_09890"
     CDS             <1..859
                     /locus_tag="VIW27_09890"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5740025.1"
                     /translation="FGHYIPPELVDEMSASPEEYSLEGENREMTVLFSDVRGFTSISE
                     GMDPKQLTRLMNALLTPMTRVIHKNRGTIDKYMGDAIMAFWGAPLANSEHARHALYAA
                     MEMIEELKVMQADFQNRDWPAVNIGIGLNTGDMNVGNMGSEFRMAYTVLGDAVNLGSR
                     LEGLTKEYGVSIIVSETTRAAIPEFVFRELDLVRVKGKKEPVAIFEPVGHRNDLDKSV
                     TSELAEYRKALMNFRAQSWDKAELDFFNLNRSNPERALYQVYLERIAVYRRNSPGEGW
                     DGVFTHTTK"
     gene            complement(875..2116)
                     /locus_tag="VIW27_09895"
     CDS             complement(875..2116)
                     /locus_tag="VIW27_09895"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017103128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEY5740026.1"
                     /translation="MQFSDFGEHFNGYSGITHLMDDLSAGLAQPGMVMLGGGNPAVIP
                     EVNAVLGKVLQQLQQSGDLLGALSNYDAPQGRAGFLKVLAEFFSQRYGWPISSRNIAL
                     THGSQSSFFILFNSFAGTARGTRKHVLIPLVPEYIGYCDVGLQADTIVSQQAKIQLLE
                     DGFFKYQVDFEQLQVDDSVGLICVSRPTNPSGNVLTDDECGQLDRLARDRQVPLLIDN
                     AYGTPFPNIIFSDVNPLWNDNCIVCMSLSKLGLPGARTGIVIASEELIRYFSNMTAIT
                     SLAPAGLGAEIVSRLILDDALQPLCDDIIRPFYQQRARLAVSLLREAIDDPRLRIHRP
                     EGSMFLWLWFEDLGITTTELYQRLKRNGLLVVPGKYFFPGQQDPSAHGECCIRMSYAQ
                     SESDLQKGIGILARELRACWA"
     gene            2265..2675
                     /locus_tag="VIW27_09900"
     CDS             2265..2675
                     /locus_tag="VIW27_09900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5740027.1"
                     /translation="MQYTHELEFKVRDYECDMQGVVNNGVYQNYLEHARHEFLQTRGM
                     NFAQITASGINLVVIRAELDYKKSLVSNDRFVVCSTLQRASRLRFQFSQDIYRLPDRQ
                     LMLSALITGAATDARGRPCLPAALQALFEDSDSP"
     gene            2827..3612
                     /locus_tag="VIW27_09905"
     CDS             2827..3612
                     /locus_tag="VIW27_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY5740028.1"
                     /translation="MGLMTGKKALIVGIASNRSIAYGIAQAMHREGAELAFSYPNDKL
                     KPRVEKFAAEFGSDIVLPCDVTIDADITGLFDGLAGHWSALDVLVHSVAFAPREALSG
                     DYLENLDREGFRVAHDISSYSLAALAKAARPMMQNTNGAIVTMSYLGSERALPNYNIM
                     GVAKASLEANVRYLSAALGPEGIRVNSISAGPIKTLAAAGIGNFKKILDEVASNAPLR
                     RNVTIEDVGNAAAFLCSDLAAGITGENIYVDSGYNIVSMGVLE"
     gene            complement(3626..>4111)
                     /locus_tag="VIW27_09910"
     CDS             complement(3626..>4111)
                     /locus_tag="VIW27_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740029.1"
                     /translation="VFLRVNEHRDAQQQSLEEVRETVIQDLRRVRTNELANAAGSAGL
                     DELEAGKSLQLLAEEWNAQVEDLGFVGRNQAEIDAAIRNRAFGMGKPDQGPLFNGLAL
                     GNGEYVIVELSAVQSNEDASDEQSLNGLTAAVGEADFRAAVDVLSSRAEVTRTPIDEL
                     Q"
BASE COUNT          841 a         1275 c         1220 g          775 t
ORIGIN      
        1 gttcgggcat tacattccgc ccgaactggt cgacgaaatg agcgcgtcgc ccgaagagta
       61 ctcgctcgag ggcgagaacc gcgaaatgac ggttctgttt tcggacgtgc gcggcttcac
      121 gtcgatttcc gagggcatgg accccaagca gcttacgcgg ttgatgaacg cgctgctgac
      181 gccgatgacg cgcgtgatcc acaagaaccg cgggaccatc gacaagtaca tgggcgatgc
      241 gatcatggcc ttctggggag cgcctctggc caactccgag catgcccggc acgcactcta
      301 cgcggccatg gaaatgatcg aggaactcaa ggtcatgcag gccgatttcc agaatcgcga
      361 ctggcccgcg gtcaatatcg gcatcggcct caacaccggc gacatgaacg tcggcaacat
      421 gggatctgaa tttcgcatgg cctataccgt gctcggtgac gcggtcaacc tcggttcgag
      481 gctcgagggc ctgaccaagg agtacggcgt tagtatcatc gtcagcgaaa ccactcgcgc
      541 ggcaatcccg gagttcgtgt ttcgcgaact cgacctggtg cgggtcaagg gtaaaaaaga
      601 gccggtggca atcttcgaac cggtcggcca ccgcaacgac ctcgacaaat cggtgacctc
      661 ggaactcgcc gagtaccgca aggcgctgat gaattttcgc gcccagtcct gggacaaggc
      721 cgagctggac tttttcaacc tcaaccgcag caacccggag cgcgcgcttt accaggtcta
      781 cctggaacgc atcgccgtct accgcaggaa ttcgcccggc gaaggctggg acggcgtgtt
      841 cacccacacc accaagtagc ctcccgacgg gcccctaggc ccagcaggcg cgcagttcgc
      901 gcgccaggat accgataccc ttctgcagat cggactcgct ttgcgcatag ctcatgcgta
      961 tgcagcactc cccgtgcgct gacgggtctt gctggccagg aaaaaaatac ttgcccggaa
     1021 ccaccagcaa gccgttgcgc ttgagccgct ggtacaactc ggtggtcgtg atcccgagat
     1081 cttcgaacca cagccacagg aacatcgagc cctcgggccg gtgaatgcgc aggcgcgggt
     1141 cgtcgatcgc ctccctcagc aggctgacgg ccagccgcgc gcgttgctgg taaaagggcc
     1201 gaatgatgtc gtcacagagc ggctgcaaag cgtcatcgag gatcagccgg cttacgatct
     1261 cggcgccgag tcccgcgggc gccaggctgg ttatcgcggt catgttcgaa aaataacgaa
     1321 tcagctcctc gctggcgatc acgataccgg tgcgggcgcc cggcagcccg agcttcgaca
     1381 ggctcatgca aacgatacaa ttgtcgttcc acaacgggtt gacgtcggaa aaaatgatat
     1441 tcggaaacgg cgtgccatag gcgttatcga tcaacagcgg cacctgccga tcacgcgcca
     1501 ggcggtccag ttggccgcac tcatcatcgg tcagcacgtt gccgctggga ttggtgggtc
     1561 gcgacacgca gatcagaccc acggagtcgt ccacctgcag ttgttcgaag tcgacctggt
     1621 acttgaaaaa accgtcctcg aggagctgta tcttcgcctg ctggctgacg atcgtgtcgg
     1681 cttgcaggcc gacgtcgcag tagccgatat actcgggcac cagcggtatc aacacgtgtt
     1741 tacgggtacc ccgcgcggtg cccgcgaagc tattgaagag gataaaaaac gaactctggc
     1801 tgccatgggt cagggcgata tttcgactgc tgatcggcca gccgtagcgc tggctaaaaa
     1861 actccgccag cacttttaga aaaccggccc tgccctgcgg cgcatcgtag ttggacaacg
     1921 caccgagcag gtcgccggac tgctgcaatt gctgcaatac ctttccgagc actgcattca
     1981 cctcgggtat gaccgcggga ttaccgccgc ccagcatgac catgccgggc tgcgccagtc
     2041 ccgcgctcag atcgtccatc aggtgggtga tacccgagta accgttgaaa tgctcgccga
     2101 aatctgaaaa ttgcatggct gctgccgtct ggcggtaacg ataacgaaga acgatagtat
     2161 atcgaaaaaa cgtcgcgctt ttgtttgaat ccgcgagcgt cgagcgcagt tgactggctt
     2221 gccccgatcc ggcaacaatg gcgcctgatc aacgcggggc ggcgatgcaa tatacccacg
     2281 aactcgaatt caaggtacgc gactacgaat gcgatatgca gggagtcgtc aacaacgggg
     2341 tttatcagaa ttacctcgag catgcacgcc acgagtttct gcagacacga ggcatgaatt
     2401 tcgcgcagat caccgcctcg ggcatcaacc tggtggtgat tcgcgccgag ctggactata
     2461 agaaatccct ggtcagcaat gaccgtttcg tggtctgcag tacgctgcaa cgggcctcca
     2521 ggctgcgttt ccagttcagc caggatatct atcgactacc cgaccggcag ctgatgctga
     2581 gcgcgctgat caccggtgcc gccaccgacg cgcgcggccg cccgtgcctg ccagccgcac
     2641 tgcaggcact tttcgaggac agcgattcac cgtgagcggg ccggcgacag gtgtgccaag
     2701 ctgtgccgtc gcagtgcctg cggaccgatt cggcagccgc ggtggcggtg gcgtcgagcg
     2761 ctcaagtttg tgattgcacc acgggcggga cgctgacaga atagttttta ttttgcgggg
     2821 aacacaatgg gtttaatgac cggaaagaag gcgttgatcg tcggtatcgc cagcaaccgt
     2881 tcgatcgcct acggaatcgc gcaggccatg caccgcgagg gcgccgagct tgcgttcagt
     2941 tatcctaacg acaagctcaa gccgcgggtc gaaaaattcg ccgccgagtt tggttccgac
     3001 atcgtgcttc cctgcgacgt caccatcgac gcagacatca ccggcctgtt cgacggcctc
     3061 gccgggcact ggtcggcact cgacgtgctg gttcactcgg tcgcgttcgc gccgcgcgag
     3121 gcactttccg gcgactatct cgaaaacctc gatcgcgagg gattccgtgt tgcccacgac
     3181 atcagctcct acagcctggc ggcgctggcc aaggccgcca ggccgatgat gcagaatacc
     3241 aacggcgcga tcgtgaccat gagttacctg ggttccgagc gtgcgctgcc gaattacaac
     3301 atcatggggg tggccaaggc gagcctggag gccaacgtcc gttacctgtc ggcagccctc
     3361 ggacccgagg gaatacgcgt gaattcaatt tccgcgggcc cgatcaagac cctggccgcg
     3421 gccggcatcg ggaatttcaa gaaaattctc gacgaggtag ccagtaatgc gccgctgcgt
     3481 cgcaatgtca ctatcgagga tgtcggcaac gcggcggcct tcctgtgctc tgacctggcc
     3541 gccggtatca ccggggaaaa tatctacgtc gattccggct acaatatcgt cagcatgggc
     3601 gtgctggagt agccgcagcc ggcgtttact gcaactcgtc tatcggcgtt cgcgtcacct
     3661 cggcgcggct cgacaacacg tcgaccgccg cccgaaagtc ggcctcgccg acggccgccg
     3721 tcaacccgtt cagagactgc tcgtcgctag cgtcctcgtt cgactgcacc gcggacagtt
     3781 cgacgatcac atactcgccg ttgccgagtg ccaggccatt gaacagtgga ccctgatcgg
     3841 gtttgcccat gccgaaagcg cggttacgaa tcgcggcgtc aatctcggcc tggttgcggc
     3901 cgacgaaccc cagatcttcg acctgcgcgt tccattcctc cgccagcagc tgcagggatt
     3961 tgccggcctc gagttcgtcg agaccagcgc tgccggcagc gttcgccagt tcgttggttc
     4021 gaacccgtct caggtcctgg atcaccgttt cacgaacctc ctcgagcgac tgctgctgcg
     4081 cgtcacgatg ctcgttgaca cgcaggaata c
//
LOCUS       DATCZV010000439         6344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_724480, whole genome shotgun sequence.
ACCESSION   DATCZV010000439 DATCZV010000000
VERSION     DATCZV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724480"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VIW27_09915"
     CDS             <1..527
                     /locus_tag="VIW27_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5740030.1"
                     /translation="IFLLAIGWCLGLAEGAERLGLSYEIGAFIAGVSIARSPIALFIA
                     ENLKPLRDFFLIIFFVALGAGFNLGLLPQIWLAALGLGLIALLLKPALYRLLMMLSKE
                     SAQRSTEVAIRLGQMSEFSLLLGVMASNLALVRAEVSYLIQISTLLSFVFSSYYIIAR
                     YPTPIALDDKLRRD"
     gene            1000..1548
                     /locus_tag="VIW27_09920"
     CDS             1000..1548
                     /locus_tag="VIW27_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY5740031.1"
                     /translation="MKTAAITIALTLFAVAGSAAAGGKSHRSPGHGVGEQLFDYARVV
                     DVQPRYRRIEVSEPVRECWEEPVYQTRNSAGGMLAGGLIGGIVGHQLGKGRGQRVATA
                     VGTLIGASIGHEAVNSHRGHGEERVAGYRQHCNTHYRTSYEEVVDSYDVTYKYRGRIY
                     HTEMPYDPGKRIKMRIQITPVI"
     gene            1577..2263
                     /locus_tag="VIW27_09925"
     CDS             1577..2263
                     /locus_tag="VIW27_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5740032.1"
                     /translation="MRAIVIEDDIDIQRQIVERLKSEGFAVDSAANGDEGLYLVQEYP
                     ADVAIIDLGLPKMSGLEVIRRIREQGNRLPILILTARGRWQDKVEGLDAGADDYLVKP
                     FHHEEMMARIRVLIRRASGWSDSRIVCTPVVLDTASQRTYVDQRELDLTAYEYKVLEY
                     LMLHAGEVISKTRLTEHLYDDESDNDSNVIEVFIRRLRQKLDPEESLKPIETLRGRGY
                     RFNLARADNR"
     gene            2270..3616
                     /locus_tag="VIW27_09930"
     CDS             2270..3616
                     /locus_tag="VIW27_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5740033.1"
                     /translation="MKSIKTRLTLVSMVVLSAFMVLTAVALERAVVKRALQAEEDGLQ
                     LLVYSLLAAVDADDRGHSITVTAERLFEPGLVTRNSGLYALIYDAGKREIWRSRSATV
                     EFGALDEIGIGEWLFETLEYQSTPYFRLGFGIQWPDINAELQRYDVVVWRNASDYVEQ
                     LSRFRQTLWAWLIVTTLLLLLVIYLVTRWSLLPLRRIGLEVKAIEDQRQTGFERSYPD
                     EIAPLTENLNILLKREQFQRERYRNAMDDLAHSLKTPLAVLTGLGDRQDLDRAQLDTL
                     REQTERMNQIVSYQLQKAASVSNIRIARPVDLVALIDRLRSALEKVYREKNIRVERDL
                     PERMLLRMDEGDCLEVIGNLLDNAFKYGRRRITITGSVDADKPAGLAIEDDGDGLDES
                     EIEQILNRGTRLDEATEGQGIGLAVVADIVESYNIRLHFSRAELGGLRVELEFRSI"
     gene            complement(3992..4195)
                     /locus_tag="VIW27_09935"
     CDS             complement(3992..4195)
                     /locus_tag="VIW27_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740034.1"
                     /translation="MQCPRCRSQMFIAEETTNSRSHVTFYRCSLCVGEHVSSAPVERE
                     PTQESTDYSLLSMSTQRVRPQFA"
     gene            complement(4385..5668)
                     /gene="purD"
                     /locus_tag="VIW27_09940"
     CDS             complement(4385..5668)
                     /gene="purD"
                     /locus_tag="VIW27_09940"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5740035.1"
                     /translation="MKILIVGGGGREHALAWKCAQSQLVERVYVAPGNAGTGLEDGIE
                     NVAIAAEDIDALLEFAAGNDIGLTIVGPEAPLVAGIVDRFEARRLKIFGPSANAAQLE
                     GSKTFTKDFLQRQHIPSAAYRSFTDIDAAREYLRDRSAPLVVKADGLAAGKGVIVAQT
                     IAEAEAAVDDMLAGNRFGDAGHRVVIEEFLDGEEASYICIVDGEEVLPMASSQDHKAR
                     DDGDRGPNTGGMGAYSPAPIVDAEIEQQVMRDIIMPTVRGMLAEGNRYRGFLYAGLMI
                     GKDRVARVLEYNCRFGDPETQPILMRLQSDLVELCLAACDGRLAAQSARWDPRDSLGV
                     VMAAAGYPGDYAKGETITNIPAESATGKVFHAGTRLDAQGRILSAGGRVLCAVGLGDD
                     IADAQREAYGLVAEIDWPSAFYRRDIGFKALRKRD"
     gene            complement(5711..>6344)
                     /gene="purH"
                     /locus_tag="VIW27_09945"
     CDS             complement(5711..>6344)
                     /gene="purH"
                     /locus_tag="VIW27_09945"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5740036.1"
                     /translation="GIIAFNRPLDEATAKAIVEQQFVEVIVAPGVDEAASAAVAAKEN
                     VRLLRCEPWGESPPRFDYKHVNGGLLVQNADLDLHRELRVVSQMQPDDAQLRDLVFAW
                     QVAKFVKSNAIIYARDTMTIGVGAGQMSRINSARIAAIKAEHAGLEVFGSVMASDAFF
                     PFRDGIDAAAEVGISAIIQPGGSMRDDEVIAAADEHGMAMVFTGMRHFRH"
BASE COUNT         1210 a         1896 c         1975 g         1263 t
ORIGIN      
        1 atattttcct gctcgccatc ggctggtgcc tgggcctcgc cgagggcgcc gagaggctag
       61 ggctgtccta tgagatcggc gccttcatcg ccggggtcag catcgcgcgc agcccgatcg
      121 cgctgttcat cgccgagaac ctgaagccgc tgcgagattt cttcctgatc attttcttcg
      181 tcgctctcgg cgccggcttc aatctcggcc tgctgccgca gatctggctg gcggcgctcg
      241 gcctcggatt gatcgcgctg ctgctcaaac ccgcgctgta ccgcttgctg atgatgctga
      301 gcaaggaatc cgcgcaacgc tccaccgagg tcgccattcg cctcgggcag atgagtgaat
      361 tctcgctgct gctcggcgtg atggcgtcca acctggcgct ggttcgagcc gaggtctcct
      421 acctgatcca gatatcgacg ttgttgtcgt tcgtgttttc ttcttactac atcatcgccc
      481 gctatccgac gccgatcgcg ctcgacgaca agttgcgtcg ggattaggtc tgcctggccg
      541 tctgccggca acataacatt cgaaggtctg caccccgtgc ggccttcggg cgaacagctg
      601 agtgtcgcct ggccctgaaa tagattgcgg ctcgggggcc gtaatgacga ttgaccgggt
      661 caagccggtc tccgcaatga cgttaggccg ggtcatgccg gcccacgcaa tgacgcttga
      721 ccaggtcatg ccgtcccggg cgatgacgtt tgactgggtc atgccggccc cgcgccggca
      781 tccatgctgg acttaccgcc gttgctggtg cgagacgatt gcagatcttg ttcgtaaacc
      841 aattggatta cggctcgggg gccgcaatga cgggcatttc tgtggcggcc gtgcacagtt
      901 aacaaattca cataaatctc ggcaaaacaa tgcgttaagc ttcgattcag taatcgctgc
      961 ctatattggg gcgcattcct tgatacgcag gagagcgcaa tgaaaaccgc agcaatcacc
     1021 atcgcattaa ccctgttcgc cgttgccggc agcgccgcgg ccggcggcaa gtctcaccgt
     1081 tcgccagggc atggtgtcgg cgagcagcta ttcgattatg cgcgagtcgt cgacgtacag
     1141 ccgcgctatc gccggatcga ggtgtcggag ccggtacgcg aatgctggga agagcccgtc
     1201 taccagactc gaaactcggc cggcggcatg ctggccggcg ggctgatcgg gggtatcgtc
     1261 ggccaccagc tgggcaaggg aagagggcag cgcgtggcaa ccgccgtggg tacgctgatc
     1321 ggtgccagta tcggccacga agccgtcaat agccatcgag gccacggcga ggaacgggtc
     1381 gccggctacc ggcaacactg caacacgcat taccggacca gctatgaaga agtggtggat
     1441 tcgtacgacg taacctataa gtaccgggga agaatctatc atactgaaat gccctatgat
     1501 cccggcaaac ggataaaaat gcgcattcag attacgcctg tgatctaatc gtttaactgt
     1561 tgacgcgaga tttgccatgc gagcaatagt catagaagac gatatcgaca ttcagcgaca
     1621 aatcgtcgag cgcctgaaat ccgaggggtt cgcggtcgac agcgcggcta acggcgacga
     1681 gggtctttac ctcgtacagg agtaccctgc cgacgtcgcc atcatcgatc tcgggctgcc
     1741 gaagatgtct ggactcgagg tgatcaggcg tatccgtgaa cagggaaacc ggctgccgat
     1801 cctgatcctg accgcccgcg ggcgctggca ggacaaggtc gaggggctgg acgccggcgc
     1861 cgatgattac ctggtcaagc ctttccatca cgaagaaatg atggcccgca ttagagtgtt
     1921 gatccggcgc gcctccggct ggtcggactc gcgcattgta tgcacgccgg tggtactgga
     1981 taccgcctcg cagcggactt atgtcgacca gcgcgaactc gacctgaccg cctacgagta
     2041 caaggtactt gaatacctga tgctgcacgc cggcgaggtg atttccaaaa cccggctgac
     2101 cgagcatctc tacgacgacg agtcggacaa cgacagcaac gtcatcgaag tattcattcg
     2161 gcggctgcgg caaaagctcg accccgagga gtcgctgaaa ccgatcgaga cgctgcgcgg
     2221 gcgcggctac cgcttcaacc tggcgcgtgc cgataaccgc tgaggcgccg tgaaatcgat
     2281 caaaaccagg ctgacgctgg tatcgatggt ggtgctgtcg gcgttcatgg tgctgactgc
     2341 cgtggccctg gaacgggcgg tggtcaagcg cgccctgcag gccgaggaag acggcctgca
     2401 actgctggtc tacagcctgc tggcggcagt cgatgccgat gatcgcgggc acagcatcac
     2461 ggtgaccgcc gagcgcctgt tcgagcccgg gctggtgacg cgcaactccg gcctctacgc
     2521 gttgatctac gatgctggca agcgcgaaat ctggcgctcg cgctcggcca ccgtcgagtt
     2581 cggcgcgctc gatgaaatcg gcatcgggga gtggctgttc gagactctcg aataccagtc
     2641 gacaccttat ttcaggctcg gcttcggcat ccagtggccg gatatcaacg ccgaattgca
     2701 acgctacgat gtcgtggtct ggcgcaacgc ctccgattac gtcgaacagt tgagccgctt
     2761 tcggcaaacc ttgtgggcct ggctgatcgt aaccacgttg ctgttgttgc tggtcattta
     2821 cctggtcacc cgctggagcc tgctgccgct gcggcggatc ggtctcgagg tcaaggcgat
     2881 cgaggaccag cggcaaaccg ggttcgagcg aagttacccc gacgaaatcg cgccgctgac
     2941 cgaaaatctg aacatcctgc tgaagcgcga acagtttcag cgcgaacgtt atcgcaacgc
     3001 gatggatgac ctggcgcaca gcctgaagac gccgctcgcg gtgctgaccg ggctcggcga
     3061 tcgccaggat ctcgatcgcg cgcagctcga taccctgcgc gagcagaccg agcgtatgaa
     3121 ccagatcgtc tcctatcagc tgcagaaagc cgccagcgtg tcgaatattc gcatcgccag
     3181 gcccgtcgat ctcgtcgcac tgatcgacag gctgcggtcg gcgctggaaa aggtttaccg
     3241 cgagaaaaac attcgtgtcg aacgcgatct gcctgaacga atgctgttgc gcatggacga
     3301 gggtgattgc ctggaagtga tcggcaacct gctggataac gcgttcaagt acggccgccg
     3361 gcgaatcacg atcactggca gtgtcgatgc cgacaaaccg gccgggctgg cgatcgagga
     3421 tgacggcgat ggcctcgacg agtccgaaat cgaacagatc ctgaatcgcg gcacccgtct
     3481 cgacgaggcg accgagggcc agggtatcgg cctcgcggtg gtggccgaca ttgtcgagtc
     3541 gtataacatt cgcctgcatt tttcgcgagc ggaactcggc ggactcaggg tcgagctgga
     3601 atttcggtcg atctagcagt ctgctggcac tcgtctcgac gggctcggta acgccgcaaa
     3661 ccagatacct gcaggcagcg gaaaacgttc gttcagcgcg tgtgaaccgt gcttcgaagg
     3721 gactcgatgt ttgaataccc ctggcctgag gcgatccgat agctgccgtg gcctgaattg
     3781 actggtctgg taatcgtgct accggtatcg acagactatt gttgtcgagg cgctgccttc
     3841 aggctgcgag cagcaggcgc aagccgtgga tcgggatgat tgtagcgggc aggagtagcg
     3901 gtcgttgcag tagttcgacg ctctcgcgag gccgattgtg ggatcttgca aaactcctgc
     3961 agacaagggc cctggcgcgg gccgtcgacg atcaggcgaa ctgcgggcga actctttgcg
     4021 tgctcatcga cagcagggaa tagtcggtcg attcctgtgt cggttcacgc tccactggcg
     4081 ccgacgaaac atgttcgcca acgcagagac tgcagcggta aaaggttacg tgactgcgcg
     4141 aattggtggt ttcttcggcg atgaacatct gcgaacggca cctgggacat tgcatgtcgg
     4201 aaatccctcg agtggtttgc gcaagtataa ccagataaat tcaaatcaca atatgttgtt
     4261 ataattatta tttaaacata caatatgtag tggttgtgtc gatttcacag ggacgtgatt
     4321 tttttctcag cgcagtcaat cacttacgct acaaagctca tttccggttt aagttcgggc
     4381 tgattcagtc gcgcttgcgc agtgccttga agccgatatc gcggcgataa aatgcgctcg
     4441 gccagtcgat ttccgcaacc agtccgtagg cttcgcgctg cgcgtcggcg atgtcgtcgc
     4501 cgaggccgac cgcacacagc acgcggccgc cggcggacag gattcgtccc tgcgcgtcga
     4561 gccgggtgcc ggcgtgaaac accttgccgg tcgccgattc ggcgggaata ttggtgatcg
     4621 tttcaccctt tgcataatcc cccgggtaac cggccgccgc cattaccacg ccgaggctgt
     4681 cgcgcggatc ccagcgcgcg gactgcgcag cgagccggcc gtcgcaggcc gcgagacata
     4741 attcgaccag gtcggattgc agacgcatca ggatcggttg ggtctccggg tcgccgaacc
     4801 ggcaattata ttccagcacc cgtgcgacgc gatccttgcc gatcatcagt ccggcgtaga
     4861 gaaaaccgcg gtagcggttg ccctcggcaa gcatgccgcg caccgtgggc atgatgatat
     4921 cgcgcatcac ctgctgctcg atttcggcgt cgactatcgg cgccggggag taggcgccca
     4981 tgccgccggt attgggaccg cgatcgccgt cgtcgcgtgc cttgtggtcc tgcgagctgg
     5041 ccatcggcag gacttcctca ccgtcgacga tacagatgta actggcttcc tcgccgtcga
     5101 gaaattcctc gatgaccacg cggtgaccag cgtcgccgaa gcgattgccg gccagcatgt
     5161 cgtcgaccgc ggcttcggcc tcggcaatgg tttgcgcgac gataacgccc ttgccggcgg
     5221 ccaggccgtc ggccttgacg acgagcggcg ccgagcggtc gcggaggtat tcgcgggccg
     5281 cgtcgatatc ggtgaagctg cggtaagcgg cgctcggaat gtgctggcgc tggagaaaat
     5341 ccttggtgaa ggtctttgaa ccctcgagtt gcgcggcatt cgcggaaggt ccgaaaattt
     5401 tcagtcggcg ggcttcgaag cgatcgacga tgccggccac cagcggtgcc tcgggtccga
     5461 cgatggtcag gccgatgtcg ttgccggcgg caaactcgag cagcgcgtcg atatcctcgg
     5521 ccgcgatagc gacattttcg atgccgtcct cgagcccggt tccggcatta ccgggcgcga
     5581 cgtagactcg ttcgacgagt tgcgactgcg cgcatttcca ggccagcgca tgttcgcgac
     5641 cgccgccgcc cacgattaat attttcatcg atccgttcac tggtactggc cggctactcc
     5701 gattttccgg ctagtggcga aaatgccgca tgccggtgaa caccatcgcc atgccgtgct
     5761 cgtcggccgc ggcgatcacc tcgtcgtcgc gcatcgagcc gcccggttga atgatggcgc
     5821 tgatgccgac ctcggcagcc gcgtcgatac cgtcgcgaaa cggaaagaat gcgtccgagg
     5881 ccatcaccga accgaagacc tcgaggccgg catgttccgc cttgatcgcg gcgatgcgcg
     5941 cgctgttgat gcgcgacatc tgcccggcgc cgacgccgat agtcatcgtg tcgcgggcat
     6001 agatgatcgc gttcgatttg acgaacttgg cgacctgcca ggcgaacacc aggtcgcgca
     6061 gctgcgcgtc gtcgggctgc atctggctga cgacccgcag ctcccggtgc agatccaggt
     6121 ccgcgttctg taccaacagg ccgccgttga catgcttgta atcaaagcgc ggcgggcttt
     6181 cgccccacgg ttcgcagcgc agcaggcgca cgttctcttt cgccgcgacc gccgccgatg
     6241 ccgcctcgtc gacgccgggg gcgacgatca cctcgacgaa ctgctgttcg acgatcgctt
     6301 tcgcggtcgc ttcgtcgagc ggtcggttga aggcgatgat gccg
//
LOCUS       DATCZV010000440         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_567251, whole genome shotgun sequence.
ACCESSION   DATCZV010000440 DATCZV010000000
VERSION     DATCZV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567251"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VIW27_09950"
     CDS             <1..490
                     /locus_tag="VIW27_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740037.1"
                     /translation="RISSPPWHAPPRAACQAAWSVKLPVCPRPPAPAIESAGFEIRAT
                     GCNPYGPATGAARGFRERREISKQSLTGTAMEQSITGVVVLAVIVLICSIIAHSLQKQ
                     FWIAVGLASLASTALLQVIVIFRAGYLDAYFPLAIAITLVISALCAALVGLIFIVFRT
                     LR"
     gene            complement(587..1849)
                     /locus_tag="VIW27_09955"
     CDS             complement(587..1849)
                     /locus_tag="VIW27_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5740038.1"
                     /translation="MIILAMYVGIFVAAYLAVSFLSRGRERTGFNSLKTVTFGDESAV
                     TPNRAAAFISVITIFLIWGAFTDSKLVPFHVSGPFIGETSFTYTAQNAAGDKDDGLVS
                     IVVHKIGEKPEQPEVEEGEGFVRNDRALVGAWRSKLINPKRNDDGSKEDGYRIIAVDG
                     KPIAPGQSVKVADGEVSLTDKQSFNFTPLKGLQLEPIWMPAPEAVVQRFIDIARDGYQ
                     NFGLWEHLGWSLFRVVLGFLLGALVGIPLGYAMGLSGWFRGWFDPIVEFMRPVPPLAL
                     IPLVIIWFGIWETGKIVLLFLASLWIMTIAARAGVSGINISKVHAAYSLGASKRQLML
                     HVIVPNSLPEIFTGARVAMGVCWGTVVAAELVAAQKGAGMMIIAASKFQLTDIVLMGI
                     IMIGIIGYGIDILMRKTERWLVPWKGRH"
     gene            complement(1846..>2498)
                     /locus_tag="VIW27_09960"
     CDS             complement(1846..>2498)
                     /locus_tag="VIW27_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5740039.1"
                     /translation="IVAGFRAPTKGTVSLNNEVVTGPGRDRGMVFQQGALFEWMSVRE
                     NIAFGPKMKHTPQAQIDEVVDHLLATVGLQDFGEKAVYELSGGMQQRVALARCLANEP
                     EVILMDEPLGALDALTREKMQGLVLKLWKETGKTIILITHSVEEALLLGERLLVMAPR
                     PGRIHKEYRLPFAEHGVDQDLREVKKSDGFADRREEILSMIWEMEEEIMGRAEEGA"
BASE COUNT          464 a          825 c          733 g          476 t
ORIGIN      
        1 gcgtatctcg agcccgccct ggcacgcgcc gccgcgagcg gcctgccagg ctgcgtggtc
       61 tgtgaaatta ccggtttgcc cgcgccctcc ggcaccggcc attgaatccg caggctttga
      121 aattcgcgcg accgggtgca atccttacgg ccctgccacc ggcgcggcca ggggattccg
      181 ggaacgccgg gaaatcagca agcagtcgct aacgggcacg gctatggagc aatcgattac
      241 cggcgtagtg gttctggctg tcatcgtgct gatctgctcg atcatcgcac acagcctgca
      301 aaaacagttc tggatcgcgg tcgggctggc gtcgctggcc tcgaccgccc tgctgcaggt
      361 catcgtcatt ttcagggccg ggtatctgga tgcttatttt ccgctcgcca ttgccatcac
      421 cctcgtcatc agcgccctgt gcgcggcgct ggtcggattg attttcatcg tctttcgaac
      481 actgcgttag ccgcacgggt ctgctgcccg gcaggggcat gatcaaacag cccgacaggc
      541 cgctcgaagc cgtcgcgccg gagtcaggcc gatactgccg cgccgcctag tggcgaccct
      601 tccacggcac cagccagcgc tcggtcttgc gcatcaggat gtcgatacca tagccgatga
      661 tgccgatcat gatgattccc atcaacacga tgtcggtgag ctggaacttg gacgcggcaa
      721 tgatcatcat gccggcgccc ttctgtgccg caaccagttc ggcggcaacc accgtacccc
      781 agcagacacc catggcgacg cgcgcgccgg tgaaaatttc cggcagtgaa ttgggcacga
      841 tgacgtgcag catcagctgc cgcttggacg cgcccagcga gtaggcggcg tgcaccttgg
      901 aaatattgat cccggaaaca ccggcccgcg cggcgatggt catgatccac agcgaggcaa
      961 ggaacagcag cacgatcttg ccggtttccc agatgccgaa ccagatgatg accagcggaa
     1021 tcagcgccag cggtggcacc ggccgcatga actcgacgat cggatcgaac cagccgcgga
     1081 accagcccga caggcccatc gcgtagccca gcggtatgcc gaccagcgcg ccgagtaaaa
     1141 aacccaacac gacgcggaac agtgaccagc cgaggtgctc ccagagcccg aagttctggt
     1201 agccatcgcg agcgatatcg atgaagcgct gcaccacggc ctccggcgcc ggcatccaga
     1261 tgggttcgag ctgcaatccc ttcaacggcg tgaaattgaa cgactgcttg tcggtcagcg
     1321 acacttcgcc gtccgccacc tttacgctct gccccggtgc gatcggcttg ccgtcgacgg
     1381 cgatgatgcg gtatccgtcc tccttgctgc cgtcgtcgtt gcgcttagga ttgatcagct
     1441 tcgaacgcca ggcgccgacc agcgccctgt cgttgcgcac gaaaccctcg ccttcctcga
     1501 cctcgggctg ctccggtttc tcgccgatct tgtgcaccac gatactcacc aggccgtcat
     1561 ccttgtcgcc cgctgcattc tgcgcggtat aggtgaacga ggtttcgccg atgaacgggc
     1621 cggaaacgtg gaacggcacc agcttcgaat cggtgaacgc gccccagatc aggaagatcg
     1681 tgatcaccga gatgaaagcc gcagcccggt tgggtgtcac cgcgctctcg tcgccgaagg
     1741 tcacggtctt gaggctgttg aacccggtcc gctcacggcc ccggctcaga aacgaaaccg
     1801 ccagataggc ggcgacgaat atacccacgt acatggcgag tatgatcatg cgccttcctc
     1861 cgctcgaccc atgatctctt cttccatttc ccagatcatg gagagtatct cctcgcgtcg
     1921 atcggcgaat ccatcgctct tcttgacttc gcgcaggtcc tggtcgacac cgtgctcggc
     1981 gaatggcagg cggtattcct tgtgaatccg tcccggtcgc ggcgccatca ccagcaggcg
     2041 ctcgccgagc agcagggctt cctcgaccga gtgggtaatc aggatgatgg ttttaccggt
     2101 ttccttccac agtttgagca ccaggccctg cattttttcg cgggtcagcg cgtcgagcgc
     2161 gccgagcggc tcatccatca ggatgacctc gggttcgttg gccaggcaac gggccagcgc
     2221 aacgcgctgc tgcatgccgc cggaaagctc gtaaaccgcc ttttcgccga aatcctgcaa
     2281 gcccacggtg gccagcagat ggtcgacgac ttcgtcgatc tgcgcctgcg gcgtgtgctt
     2341 catcttcggt ccgaaggcga tgttctcgcg tacgctcatc cactcgaaca gcgcaccctg
     2401 ctgaaaaacc atgccacggt cgcgaccggg gccagtaacc acctcgttgt tgagcgacac
     2461 ggtgcccttg gtcggcgccc ggaatccggc gacgatat
//
LOCUS       DATCZV010000441         5303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_778490, whole genome shotgun sequence.
ACCESSION   DATCZV010000441 DATCZV010000000
VERSION     DATCZV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778490"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VIW27_09965"
     CDS             <1..264
                     /locus_tag="VIW27_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740040.1"
                     /translation="DESQLMPASVCIWRAHKLFHQDRQIESGALLLLDERDAGHKDWL
                     RPRPNQEQANASSTSLQNETNRWYDEVRDFMKKNKIIDSSIIT"
     gene            complement(247..2427)
                     /locus_tag="VIW27_09970"
     CDS             complement(247..2427)
                     /locus_tag="VIW27_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740041.1"
                     /translation="MKKIVLLVLLGAIVLVFFQFDLGQYLTLEYVKEQQQDIDRFYAD
                     NRMLTLAGFFVVYVAVTGASLPGAAVLTLAAGAIFGLFTGVVLVSFASTIGASLAFLL
                     SRYLLRDLVQSRFGASLQSINKGIDKEGAFYLFAMRLVPAFPFFVINLVMGLTRLRLW
                     TFFWVSQVGMLAGTLVYVNAGTQLAQIDSPEEIFSPGILLSFLLLAALPFIGRRVVSV
                     IAARKALAGYEPPEEFDTNLIVIGAGSAGLVTAYIAAAVKAKVTLIEKHKMGGDCLNF
                     GCVPSKALIRSAKFMSQVARSREFGIARADAEFDFAEVMERVQRVVKTIEPHDSVERY
                     TGLGVDVVLGEARITSPWSVEVNGETIRAPNIVVASGGRPFVPPIEGIDTVDYYTSDN
                     LWDIRRKPQKMTVLGGGPIGSELAQAFARLGVEVTQVEMLPRLLSREDPEISELVERR
                     FTEEGIRVLTGHTAKKFVKRGGKDWLIAEHDGDKVEIEFDTLLVAVGRSPNSRGFGLE
                     ELGVRLNANGTIEVNDYLQSSMPTIYACGDVAGPYQFTHVGAHQAWYTSVNSLFGIFR
                     RFKADYSVIPWATFTEPEIARVGLNEADAREQGIEYEVTRYGIDDLDRAIADSEAHGF
                     VKVLTVPGKDRILGVTIVGEHAGDLIAEYVLAMRHGLGLNKILGTIHIYPTLAEANKY
                     AAGEWKRAHAPARLLEWLAKFHRWRRKGRAGRAAISAAGSGDDR"
     gene            complement(2481..3581)
                     /gene="mnmH"
                     /locus_tag="VIW27_09975"
     CDS             complement(2481..3581)
                     /gene="mnmH"
                     /locus_tag="VIW27_09975"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY5740042.1"
                     /translation="MREDTSDYRQLFLRDIPLLDVRAPVEFSHGAFPNAVNLPLLDDA
                     QREQIGKRYKDAGQDEAIRLGLELATPQIREQRLRDWCGFSDAHPDGYIYCFRGGLRS
                     RTTQQWLRERGVEMPLVRGGYKAMRRFLIDELENSAARVALVCVGGLTGVGKTRALLK
                     TRHHIDFEGLANHRGSAFGRDPLDRQPAVIDWENRVSIAFLKHRERFPQRPLLVEDEG
                     RCIGRVNLPDYLYAAMLRAPRALLEAGIERRVQMIREDYIERNWPEYRQVHPDDAEAE
                     FSRFVLDNLARIQKRLGGDRYRRVCKGFEAALAKLFASGDASGFDAGIRTLLEEYYDP
                     MYRYQIETKQPEIVFRGDETEFLQWAEVYCKG"
     gene            complement(3588..>5303)
                     /gene="rlmKL"
                     /locus_tag="VIW27_09980"
     CDS             complement(3588..>5303)
                     /gene="rlmKL"
                     /locus_tag="VIW27_09980"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEY5740043.1"
                     /translation="SIDLSGESLHRRGYRQRHSGAPLKEHLAAALLVQAGWSADSPGE
                     LRLIDPMCGSGTFVIEAAMMAANIAPGLERDYFGFEKWRRHDAAIWQQCLAEARAAIR
                     EPSRLDIHASDRDLQALAIARQNAARAGVERYISFSHQQIADLELPATDIETLVCCNP
                     PWGRRLDGDDLGQLYADLGDAVRRLAPARLAIFSANPELLHRLRLNRISRKPVRNGAL
                     ECLFAVFSTAAEVAVDEPPQPRRTIDDVAAEPLRNRLRKNARHLGRWAARNRVSCYRI
                     YDADLPEFAFALDRYGSDLATDKACYLLQEYQAPKTIDADKARQRILLAADLVREEFG
                     IGEQQLFCKTRSRQRGREQYRKQDNRGEFLQVREGEAALLVNLSDYLDSGLFLDHRIT
                     RQRVYREAGGKRVLNLFCYTGTVGVQAGLGGARSVLNVDLSSTYLDWARENHLLNGLD
                     DETRFGFLRADILELLRDPTRFSLDDKYDLIFFDPPSFSNSSSMRQTLDIQRDHDVLL
                     RQAMRLLAPGGLLLFSTNRRGFRLDAAFERDYRPRDISRETLPEDFKRNPGIHRCWEI
                     RQPDR"
BASE COUNT         1057 a         1693 c         1605 g          948 t
ORIGIN      
        1 gacgagagcc agctgatgcc ggcctcggtc tgtatctggc gcgcgcacaa gttatttcac
       61 caggaccggc agatcgaatc cggagcgctg ctgctgctcg acgaacggga tgccggccac
      121 aaggactggc tcaggccgcg cccgaaccag gagcaagcca acgccagctc gacctcgctg
      181 caaaacgaga ccaaccgctg gtacgacgag gtcagggatt tcatgaaaaa gaacaagatc
      241 atcgattcat cgatcatcac ctgagccggc agcactgatc gcagcgcgac cggcgcgccc
      301 cttgcgtcgc caacggtgga acttcgccaa ccattcgagc aacctcgcgg gcgcatgcgc
      361 gcgtttccac tcgccagccg cgtacttgtt ggcctcggcc agcgtcgggt agatgtgaat
      421 cgtgccgaga atcttgttga ggccgagccc gtgccgcatc gccagcacat actccgcgat
      481 caggtcgccg gcatgctcgc cgacgatagt gaccccgaga atccgatcct tgccgggtac
      541 cgtcagcacc ttgacgaaac cgtgtgcctc gctgtcggca atcgcacgat ccaggtcgtc
      601 gatgccgtag cgggtgactt cgtactcgat gccctgctcc cgcgcatcgg cttcgttgag
      661 cccgacgcgg gcaatctcgg gttcggtgaa ggtcgcccac ggaatcaccg agtaatcggc
      721 cttgaagcgc cgaaagatac cgaacagcga attcaccgac gtgtaccagg cctggtgcgc
      781 gccgacatgg gtgaactggt agggcccggc gacatcgccg caggcataaa tggtcggcat
      841 gctggactgc aggtaatcgt tgacctcgat cgtgccgttg gcgttcaggc gcacgccgag
      901 ttcctcgagc ccgaaaccgc gtgaattggg cgatctgccg accgccacca gcagcgtatc
      961 gaactcgatt tcgaccttat cgccatcgtg ctcggcgatc agccagtcct tgccgccgcg
     1021 cttgacgaat ttcttcgccg tgtggccggt gagcacgcgt atgccctcct cggtaaagcg
     1081 ccgctcgacg agctcggata tttccgggtc ctcgcgcgac agcaggcgcg gcagcatttc
     1141 cacctgcgtg acctcgaccc cgagccgcgc gaaagcctgg gccagttccg agccgatcgg
     1201 tccgccgccg agtacggtca ttttctgcgg cttgcggcga atgtcccaca ggttgtccga
     1261 agtataatag tcgaccgtgt cgatgccctc gatgggtggc acgaacggcc gcccgccgga
     1321 ggcgacgacg atattgggcg cacggatcgt ctcgccgttg acctcgacgc tccacggtga
     1381 ggttatgcgg gcctcgccga gcaccacgtc gacaccgaga ccggtgtagc gctcgaccga
     1441 atcgtgcggc tcgatcgtct tcacgacccg ctgcacgcgc tccatgacct cggcgaaatc
     1501 gaactcggcg tcggcgcgag cgatgccgaa ctcgcggctg cgggcgacct gcgacatgaa
     1561 ttttgccgag cgtatcagcg ccttcgatgg cacacagcca aaattcaggc aatcgccgcc
     1621 catcttgtgt ttttcgatca gcgtcacctt cgccttgacc gcggcggcga tgtaggccgt
     1681 taccagcccg gcggagcccg cgccgataac gatcaggttg gtatcgaatt cttcaggcgg
     1741 ttcgtagccg gccagcgcct tgcgggcggc gatcaccgac accacgcggc gaccgatgaa
     1801 cggcaacgcc gccagcagca gaaaagaaag caggattccg gggctgaaaa tctcttccgg
     1861 cgaatcgatt tgcgccagct gggttccggc gttgacgtag accagggtac cggcaagcat
     1921 gccgacctgg ctgacccaga agaaagtcca caggcgcagc cgggtcaggc ccatcaccag
     1981 gttgatgacg aaaaacggga acgccggcac caggcgcatc gcgaacaggt aaaaagcgcc
     2041 ttccttgtcg atccccttgt tgatcgactg cagcgaggcg ccgaagcggc tttgcaccag
     2101 gtcgcgcagc aggtagcgcg acagcaaaaa ggccagcgag gcgccgatgg tactggcgaa
     2161 ggacaccagc accacgccgg taaacaaacc gaagatggcg cccgcggcca gcgtcagcac
     2221 cgcggccccc ggcaacgacg cgccggtgac ggcgacgtaa acgacaaaaa acccggccag
     2281 ggtcaacatg cggttgtcgg cgtagaagcg atctatatcc tgttgctgct ccttgacata
     2341 ctccagcgtc aggtactgac cgagatcgaa ctggaagaac accagcacga tcgcgcccag
     2401 caagaccagt aaaactattt tcttcatgcg ttgttatccg ttgttatccg ttcgcgtcgg
     2461 cacgactctc gaaaaccgat tcagcccttg cagtagacct cggcccattg tagaaattcc
     2521 gtttcatctc cgcgaaatac gatttcgggt tgcttggttt cgatctggta acgatacatt
     2581 ggatcgtaat attcttcgag cagtgttcga ataccggcat cgaatcccga cgcgtcaccg
     2641 ctggcgaaaa gctttgcgag ggccgcttcg aatcccttgc aaacccttcg gtagcgatcg
     2701 ccgccgagcc gcttttgaat gcgcgcaagg ttgtcaagca cgaagcggct gaattcggcc
     2761 tcggcgtcgt cggggtgcac ctgccggtac tccggccagt tgcgctcgat gtaatcctcg
     2821 cgaatcattt gcacgcgccg ttcgatgccg gcctcgagca gtgcgcgcgg cgcccgcagc
     2881 atggccgcgt acagataatc cggcaggttg acgcggccga tacaacggcc ctcgtcctcg
     2941 accagcaacg gccgctgcgg aaagcgctcg cggtgcttta aaaaagcgat cgacacccgg
     3001 ttctcccagt cgatgaccgc cggctgtcga tccagcgggt cgcggccgaa agccgagccg
     3061 cgatggttgg cgagcccttc gaaatcgatg tgatgacgcg tcttcagcaa tgcccgggtc
     3121 ttgccgacac cggtcaggcc gccgacgcag accagcgcta cccgggctgc cgaattctcg
     3181 agttcgtcga tcagaaaacg acgcatcgcc ttgtaaccgc cgcgcaccag cggcatctcg
     3241 acaccgcgct cgcgaagcca ttgctgagtg gttcgcgagc gcaagccacc acgaaaacag
     3301 taaatatagc cgtccgggtg cgcgtcgctg aagccgcacc agtcgcgtaa tcgctgctcg
     3361 cgaatctgcg gtgttgccaa ttccagtccg agccggatcg cctcgtcctg gccggcgtct
     3421 ttatagcgct tgccgatttg ctcgcgctgc gcatcgtcga gcagcggcag attgaccgcg
     3481 tttggaaacg cgccgtggga gaactcgacc ggcgcgcgca cgtcgagcaa cgggatgtcc
     3541 cgcaggaaca attgccggta atcgctggtg tcttcgcgca tcgcggacta gcgatcgggc
     3601 tgccgaattt cccagcagcg atgaatgccg gggttgcgct tgaaatcctc gggaagagtc
     3661 tcgcgggaga tatcccgcgg ccggtaatcc cgctcgaacg cggcatcgag cctgaacccg
     3721 cgacgattgg tcgaaaacag cagcagtccg ccgggtgcca gcagccgcat tgcctggcgc
     3781 agcagaacat cgtggtcgcg ctgaatgtcg agggtttgcc gcatgctact cgaattggaa
     3841 aacgacggtg gatcgaaaaa tatcaaatca tatttgtcat ccaatgaaaa cctcgtcgga
     3901 tcgcgcagca actcgagaat atccgcgcgc agaaaaccga aacgggtttc gtcatcgagc
     3961 ccgttgagca gatgattctc gcgcgcccag tcgagatagg tcgacgacag gtcgacgttt
     4021 agtaccgaac gcgcaccgcc aaggccggcc tgcacgccga ccgtgccggt ataacagaac
     4081 aggtttaaca cccttttacc gccggcctcg cgataaacgc gttgacgcgt gatgcggtga
     4141 tcgagaaaca gtcccgagtc gaggtaatcg ctgaggttga ccagcagcgc cgcctcgccc
     4201 tcgcgcacct gcaaaaactc gccgcgatta tcctgtttgc ggtactgctc gcggccgcgc
     4261 tggcggctgc gcgtcttgca gaacaattgc tgctcgccga taccgaactc ctcgcgcacc
     4321 aggtcggcgg caaggagaat gcgttgccgg gccttgtccg catcgatggt tttcggtgcc
     4381 tggtattcct gcagcaggta gcaggcttta tcggtagcga gatcgctgcc gtagcgatcg
     4441 agcgcgaacg cgaactccgg cagatcggca tcgtagattc gataacagct gacccggttg
     4501 cgcgccgccc agcgaccaag atggcgcgcg ttcttgcgca ggcgattgcg caggggctcg
     4561 gcggccacat catcgatcgt ccgacgcggt tgcggcggct cgtcgaccgc cacctcggca
     4621 gcggttgaaa agaccgcgaa cagacactcg agtgcgccgt tcctgaccgg cttgcggctt
     4681 atgcggttga gccgcaaccg atgcagcaac tccggattgg cggagaaaat cgccaggcgc
     4741 gccggcgcca gccggcgtac ggcatcaccg agatccgcgt ataactggcc gagatcgtcg
     4801 ccatccaggc gccgtcccca cggcgggttg cagcacacca gggtttcgat atcggtcgcc
     4861 ggcagctcga gatcagcgat ttgctggtgc gagaaactga tataccgctc gactccggcg
     4921 cgcgcggcgt tttgccgcgc gatcgccagc gcctgcagat cgcggtcgct ggcatgaatg
     4981 tcgagcctgg acggttcgcg aatggccgca cgtgcctcgg cgagacattg ctgccagatt
     5041 gccgcatcgt gacgccgcca tttttcgaaa ccgaaataat cgcgctcgag accgggcgcg
     5101 atgttggccg ccatcattgc cgcctcgatt acgaaggttc cggagccgca catcgggtcg
     5161 atcaggcgca attccccggg actgtccgcc gaccagccgg cctgcaccag cagcgccgcg
     5221 gccaggtgct ccttgagtgg cgcgcccgag tgacgctggc gataaccccg ccgatgcagg
     5281 ctctcaccag acaaatcgat act
//
LOCUS       DATCZV010000442         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_567535, whole genome shotgun sequence.
ACCESSION   DATCZV010000442 DATCZV010000000
VERSION     DATCZV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567535"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VIW27_09985"
     CDS             <1..814
                     /locus_tag="VIW27_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740044.1"
                     /translation="YQRLGELAPFAVSAAMSLLLVAIFWLLRLSDKPGIVAPSERPRR
                     GPARSIGRFVAQPRLRLAWTLVFMRSSWWVFFFIYVPVYAVQHGLGEIAGAMVVSAGN
                     ALLFVAPLAGSLARRIGVRGVLRVGYFVCGGFTLAAGLFFDLPAAVITLLLLASVGTV
                     CLDAVGNVPFLAAVRSREREEMTTVFRTYLDAAELLPPAIFALVLSFAELKAVFLIQG
                     GAMLAVLALLQYLPASLGKTRILLRPEGPLPGSTAPAAAPDTAAGTLSKPAP"
     gene            complement(826..>2103)
                     /locus_tag="VIW27_09990"
     CDS             complement(826..>2103)
                     /locus_tag="VIW27_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY5740045.1"
                     /translation="KGVIADQFYAERLYNQGALAALLRQNGFIGIENKGSLQTESERN
                     QDLGMMAKRNIVLAQASKPATRAASLPRSGPLQVAVLLGDPRKQDRVKPGATFDEDDQ
                     HTIAELKKALGELDGYRFSYFDDHDRFIDNLPKWKSKFDLALNFCDEGFDNRASHELH
                     VPALLEMANIDYTGGNPQCLAYCYDKSLIRGLASEMDIPVANAYIVKPEDTTFIDITI
                     PFPVIIKPNFGDSSFGITSSSVCNNVTELENAIVASRAQFGYQCPVLVEQFLTGKDIS
                     VGVIGNPSTHFEFLPIIEEDYSLLPEGYPRICGYEAKWDPESPYWQIRSVPANLNDST
                     EQFLQDSCLKLFERVECRDYARFDWRLDDNGTPRLLEVNPNPGWCWDGHLAKMAKHAN
                     ISYAAMLGMIVDAALLRRQQAVGQQLQARVAIA"
BASE COUNT          306 a          653 c          702 g          442 t
ORIGIN      
        1 ctaccagcgt ctcggcgagc tggcgccgtt cgcggtgagc gcggcgatgt cgctgttgct
       61 ggtcgcgatc ttttggctgc tgcgcctgag cgacaaaccc ggcatcgtcg cgcccagcga
      121 acgcccgcgg cgcggcccgg cgcgcagcat cgggcgcttc gtcgcccagc cccgcctgcg
      181 cctggcctgg accctggtct tcatgcgctc gtcgtggtgg gtattctttt tcatctacgt
      241 gccggtctac gcggtgcagc acgggctcgg cgagattgcc ggcgcgatgg tggtctccgc
      301 cggtaacgcg ctgttgttcg tcgcgccgct cgccggcagc cttgcgcgcc gcatcggcgt
      361 gcgcggcgtg ttgcgcgtcg gctatttcgt ttgtggcggc tttacgctgg ccgcgggcct
      421 gtttttcgat ctgccggcgg cggtaatcac gctgttgttg ctggcgtcgg tcggcacggt
      481 ctgcctggac gcggtcggca acgtgccgtt cctggccgcg gtgcgctccc gcgagcgcga
      541 ggaaatgacc accgtgttcc gcacctacct ggacgcggcc gagttgctgc cgccggcgat
      601 attcgcgctg gtgctgtcgt ttgccgagct gaaggccgtg ttcctgatcc agggtggcgc
      661 gatgctggcg gtgctcgcgc tgttgcaata cctgcccgcg agcctcggca agacccgcat
      721 cctgctgcgc ccggaaggcc cgttgccggg ctcgaccgcg ccggccgcgg cgccggacac
      781 cgcggccggg acgctgtcga aaccggctcc ctgacgcgct gtgcttcagg cgatggcgac
      841 gcgagcttgc agctgctgac cgacggcttg ctggcggcgc agcagggccg cgtctacgat
      901 catgccgagc atcgcggcat aggagatgtt ggcgtgcttc gccattttcg cgaggtggcc
      961 gtcccagcac catcccggat tgggattgac ttccagcagt cgcggcgtgc cgttgtcgtc
     1021 cagacgccag tcgaagcgtg cgtaatcgcg gcactcgacg cgctcgaaca gcttcaggca
     1081 gctgtcctgc aggaactgct cggtggaatc attgagattc gccggtaccg agcgaatctg
     1141 ccagtagggt gactcgggat cccacttggc ctcgtaaccg cagatgcgtg gataaccctc
     1201 gggcagcagc gagtaatctt cctcgatgat cggcaggaat tcgaagtggg tcgacggatt
     1261 gccgattacg ccaacgctga tgtccttgcc ggtgaggaat tgctcgacca gcaccgggca
     1321 ctgatagccg aactgcgccc ggctggcgac gatcgcgttt tccagctcgg tgacgttgtt
     1381 gcagacgctc gagctggtga taccgaagct cgaatcgccg aagttgggct tgatgatcac
     1441 cgggaacgga atcgtgatat cgataaaggt ggtgtcctcc ggcttgacga tgtaagcatt
     1501 ggctaccgga atgtccattt cgctggccag gccgcgaatc agcgacttgt cgtagcaata
     1561 ggccaggcac tgcggattgc cgccggtgta atcgatattc gccatttcca gcagcgccgg
     1621 cacgtgcagc tcgtggctgg cccggttgtc gaagccttcg tcgcagaaat tcagtgccag
     1681 gtcgaacttg ctcttccatt tcggcaggtt gtcgatgaag cggtcgtggt cgtcgaagta
     1741 gctgaaacgg tagccatcga gctcaccgag cgctttcttc agttccgcga tagtgtgctg
     1801 gtcgtcttcg tcaaaggtag cacccggctt gacgcgatcc tgcttgcgcg ggtcgccgag
     1861 cagcaccgcg acctgcagcg gcccggagcg cggcaggctg gccgcgcgag tcgcgggttt
     1921 gctggcctgc gccagcacga tattgcgctt cgccatcatg ccgagatcct ggttgcgctc
     1981 ggactcggtt tgcagtgaac ctttgttctc gatgccgatg aatccgtttt gccgcaacag
     2041 cgcggcgagc gctccctggt tgtataggcg ctcggcgtag aactgatcgg cgatcacgcc
     2101 ttt
//
LOCUS       DATCZV010000443         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_89116, whole genome shotgun sequence.
ACCESSION   DATCZV010000443 DATCZV010000000
VERSION     DATCZV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89116"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VIW27_09995"
     CDS             <1..474
                     /locus_tag="VIW27_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5740046.1"
                     /translation="DQLQPGLRQAIQADMTSAWFMYLVLIVLVAFSVLNTQLMSVLER
                     TREFGTMLALGLKPSRLARLVMQETALMTGLGFGLGIAGGFLLTLYLSQVGFSYPGMA
                     EMAERFYLPDRMYPALSPLSLTLGPAVVSLGSLLASIYPATRLYRLTPVAAMRAV"
     gene            476..1717
                     /locus_tag="VIW27_10000"
     CDS             476..1717
                     /locus_tag="VIW27_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5740047.1"
                     /translation="MRLGPRQLSIVLTLSWRNLWRNRRRTGIMLGAISVGVWAMIFMT
                     ALMRGMVDDMLVQGIRSLPGHIQIQHPDYLDDPSVVNSIDEPRGALLAALQGGGAQRW
                     AARIRVPAVIASERESRGVELIGVQPAFEQEISGIPGKTGEGRFLESSDDGGLVIGAR
                     LAQRLETRLGKRVVIMSQDPDNNISERGYRVVGIYRAEIAALEESRVYAGLEEVQQLL
                     GIDGRVSQIIVYGEDYRDVDSRYRSLAEALPGGLDIRPWYRIDTYLAAMFEMMDGFVL
                     VWVIIIFLALSFGLVNTLVMAIFERVREIGLTQALGMRPSQIVLQILLESLLLLLIGL
                     AVGNGLAIATIKPLESGLDVSIVAEGMAMMGAGSVLYPSLTLHDLLLANGVVILLGIL
                     TSILPAWRAAHLDPIRALNMH"
     gene            1727..2410
                     /locus_tag="VIW27_10005"
     CDS             1727..2410
                     /locus_tag="VIW27_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740048.1"
                     /translation="MTAISCRQLCKTFRQGDETITGLDHVDIDIDSGEFVCLSGPSGS
                     GKTTLLNAIGGLDTPDSGEIRIGDLQVEKLDRGALADMRLHHIGFVFQSYNLIPVLSA
                     RENVEFVMQVQGVPAAERHNKAAAILREVGLEGLEDRRPAQLSGGQQQRVAVARAIVS
                     RPDLVLADEPTANLDSVASTQLMDLFAELNSNHGVTFVIATHDKRVMRYARRLIRMRD
                     GRIISDEAN"
BASE COUNT          414 a          834 c          801 g          462 t
ORIGIN      
        1 gaccagctgc aaccgggcct gcggcaggcg atacaggccg atatgaccag cgcctggttc
       61 atgtacctgg tgctgatcgt gctggtcgct ttcagcgtgc tcaacacgca gctgatgtcg
      121 gtgctagagc gcacccgcga gttcggcacc atgctggcac tggggctgaa accctcgcgg
      181 ctggcacgac tggtgatgca ggagaccgcg ctgatgaccg gtctcggttt cggtctcggc
      241 atcgccggcg gctttctgct gaccctctac ctcagccagg ttggcttttc ctatcccggc
      301 atggcggaaa tggcggagcg cttttacctg ccggatcgca tgtacccggc gctttcgccg
      361 ctgtcgctga ccctcggacc ggcggtcgta tcgctcggca gcctgctggc gtcgatctac
      421 ccggccaccc gtctgtaccg gctgacgccg gtcgccgcga tgcgggcggt ctgagatgcg
      481 gctcggccca cgccagttat cgatcgtatt gacactgtca tggcgcaacc tgtggcgcaa
      541 ccggcgccgc accggcatca tgctcggcgc gatttcggtc ggcgtctggg cgatgatctt
      601 catgaccgcg ctgatgcgcg gcatggtcga cgacatgctc gtccagggta ttcgcagcct
      661 gccgggccac atccagatcc agcaccccga ttacctcgac gaccccagcg tggtcaacag
      721 catcgacgaa ccgcgcggcg cgttgctcgc ggcgctgcag ggcggcggcg cgcagcgctg
      781 ggcggcgcgc attcgcgtac cggcggtaat cgccagcgag cgcgagagcc gcggggtcga
      841 gttgatcggc gtgcaacccg cgttcgaaca ggagatcagc ggcattcccg gtaagaccgg
      901 cgaaggccgg tttctcgaat cgtccgacga cggcggcctg gtgatcggcg cccggctcgc
      961 gcagcgcctc gaaacccggc tcggcaagcg ggtcgtcatc atgagccagg accccgacaa
     1021 caatatcagc gaacgcggtt atcgcgtcgt cggtatctat cgcgccgaaa tcgctgccct
     1081 cgaggagagc cgcgtctacg cggggctcga ggaggtgcaa caattgctgg gtatcgacgg
     1141 ccgcgtatcg cagatcatcg tctacggcga ggattaccgc gacgtcgatt cgcgctatcg
     1201 aagtctcgcc gaagccctgc ccggcggcct ggatattcgc ccctggtacc gtatcgatac
     1261 ctacctcgcg gcgatgttcg agatgatgga cggtttcgtg ctggtgtggg tgatcatcat
     1321 tttcctggcg ctgtccttcg gcctcgtcaa cacgctggtg atggcgatct tcgagcgcgt
     1381 gcgcgagatc gggctgaccc aggcgctcgg catgcgcccg tcgcagatcg tgctgcaaat
     1441 tttgctggag tccctgctgc tgctgctgat cggcctcgcc gtcggcaacg gcctggcgat
     1501 cgccacgatc aagccgctgg aaagcggcct cgacgtatcc atcgtcgccg agggcatggc
     1561 gatgatgggc gccggcagcg tgctctaccc gagcctgaca ctgcatgacc tgttgctcgc
     1621 caacggcgtg gtgatcctgc tcggcatcct gaccagcatc ctgcccgcgt ggcgcgccgc
     1681 gcacctcgac ccgattcggg cccttaacat gcattgacct acagccatga ccgcgattag
     1741 ctgccgccaa ctttgtaaga cctttcgcca gggcgatgaa accatcaccg ggctcgacca
     1801 tgtcgacatc gacatcgaca gcggcgaatt cgtctgcctg tcgggaccct ccggttccgg
     1861 caagaccacg ctgctgaacg ccatcggcgg cctcgatacg cccgacagcg gtgaaatccg
     1921 catcggcgac ctgcaggtcg agaagctcga ccgcggcgcg ctggccgaca tgcgcctgca
     1981 tcatatcggt ttcgtgttcc agtcctacaa cctgatcccg gtgctcagcg cgcgcgaaaa
     2041 tgtcgagttc gtgatgcagg tgcagggcgt gccggccgcc gagcggcata acaaagcggc
     2101 ggcgattctg cgcgaggtcg gcctcgaggg cctggaagac cggcgcccgg cgcagctgtc
     2161 cggcggccag cagcaacgcg tcgcggtggc gcgcgcgatc gtctcgcggc ccgacctggt
     2221 actcgccgac gagcccaccg ccaacctcga cagcgtcgcc tcgacacagc tgatggacct
     2281 gttcgccgaa ctcaacagca accacggggt taccttcgtg atcgccaccc acgacaagcg
     2341 cgtgatgcgt tacgcgcggc gcctgatccg catgcgcgac ggcaggatca tcagcgatga
     2401 agcgaactag cccgcgaagc gatcggccta tcgttccggt tctgctgctt agcgtttgcg
     2461 gtctgttgtt ttgcggtcag gcgatggcta tcgaaacacg cggccacctc a
//
LOCUS       DATCZV010000444         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_567610, whole genome shotgun sequence.
ACCESSION   DATCZV010000444 DATCZV010000000
VERSION     DATCZV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567610"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1245)
                     /locus_tag="VIW27_10010"
     CDS             complement(316..1245)
                     /locus_tag="VIW27_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5740049.1"
                     /translation="MSKQASVHFQRMKDLPSTHLLGTGTPMCAGCGGLEALHTLYDIL
                     GDKTVFVNAAGCMTLLSVYPFTPFRGGWLYTSMASAPAGAQGVRDALDILIAKKKLPA
                     EEDVEVVVISGDGSAYGMGMSATSSAMDRNLDFIYLCYDNEGYGNTGQQYSAATPHAA
                     LTSTSKGSHGYPGYKKDLFSIWAAHDPTYVASVIAAEPLDLARKVEKLQTLSGPRLLL
                     ALSPCPTGWDYDPSLSVEIGRLAVQTGVWPLKEYIDGEVRHTKVPRQRKPVEAYLELQ
                     GRFKHLFEPERDTEAIAEIQARVDAYWDQAARE"
     gene            complement(1256..>2137)
                     /locus_tag="VIW27_10015"
     CDS             complement(1256..>2137)
                     /locus_tag="VIW27_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HEY5740050.1"
                     /translation="NDIMAARDSGFLQIHCATCQEVADFILIAYRLAEHAEVRVPAIV
                     NLDGFYLSFTREPVELPEAQKARAFVGDYDPENLQFRGNAPLSQAIAVLGGGPYSYFR
                     YQAHLAARQALPIYDRIADEFASVFGRRYEMLDCYRSDDAEYLLVMIGSFSTKARDAV
                     DSLRAAGRKIGLVRPRLFRPFPGSRVRQLLRGRRAVAVIDQNLSMGMGGVLHSEFATA
                     LYGASDAPILASYIGGLGGRDISQQEFFAIADELQLAYESGEAPAPRLLYTDNELNEM
                     RKLQAIASAEHAEIRQS"
BASE COUNT          383 a          670 c          675 g          409 t
ORIGIN      
        1 ccgtatcgct gcgcatataa ccagtttttg cggcttgccg gagacgctcg ccggcggctg
       61 gtcgacaagg tgaaagtagc ggcggagtct gaaatccact atccagctcc tgcagtagtc
      121 gccaatctcg tctgcggtcg catactagac gccatgtaac cgcatccagg ccgcaccctg
      181 tgcgccgaga cgttcatcga ggacaatgaa tgccgcgatc ccggcgatcc tggaatgcgt
      241 aaacggcacg ctcacgatct ccagcgtcca gaggttttca ctgtgccgat gaaagttaag
      301 gctatccggt cgcggtcatt cgcgggcggc ctggtcccag taagcgtcga cgcgcgcctg
      361 tatttcggct atcgcctcgg tatcgcgctc cggttcgaac aggtgtttga agcggccctg
      421 cagttccagg taggcctcga ccggtttgcg ttgcctgggc accttcgtat gcctgacttc
      481 gccgtcgata tattctttca acggccagac gccggtttgc accgcgaggc gaccgatttc
      541 gacgctcagc gaggggtcgt aatcccagcc ggtagggcag ggcgacaatg ctagcagcag
      601 ccgcggaccg ctcagcgtct gcagtttctc caccttcctg gcgaggtcca gcggctcggc
      661 ggcaatcacg ctagcgacat aggtcgggtc atgcgccgcc cagatcgaga acaggtcctt
      721 cttgtaaccc gggtagccgt gcgaaccctt gctggtcgag gtcagcgcgg cgtgcggagt
      781 ggccgcggaa tactgttgac cggtgttgcc gtagccctcg ttgtcgtagc acaggtaaat
      841 gaaatcgagg ttgcgatcca tcgcgctcga ggtggccgac atccccatgc cgtaggccga
      901 gccgtcgccg ctgattacca ccacctcgac atcttcttcc gccggcaatt tctttttcgc
      961 gatcaggata tcgagcgcgt cgcgcacccc ttgcgcgcct gccggcgccg aggccatcga
     1021 agtatacagc cagccgccgc gaaacggcgt gaacggataa accgacagca gcgtcatgca
     1081 gccggccgcg ttgacgaaca cggttttgtc gccgaggata tcgtacaggg tgtgcaacgc
     1141 ctcgagaccg ccgcagccgg cgcacatcgg cgtgccggtg ccgagcaggt gggtcgacgg
     1201 caggtccttc atacgctgaa aatgtacgct tgcctgtttg ctcatggtaa cggcctcatg
     1261 actggcgaat ctccgcgtgc tcggcgctgg cgatcgcctg cagcttgcgc atctcgttca
     1321 gttcgttgtc ggtgtagagc agtcgcggcg ccggcgcttc gccactctcg taggccagct
     1381 gtaactcgtc ggcaatggca aagaattcct gctggctgat atcgcgcccg ccaaggccgc
     1441 cgatgtagct cgccaggatc ggggcgtcgc tggcgccgta gagcgcggtt gcgaattcgc
     1501 tgtgcagcac gccgcccatg cccatcgaca ggttctgatc gatgaccgcc accgcgcgcc
     1561 ggcctcgcag cagttgccgc acccggctcc ccgggaacgg gcgaaacagg cgcggcctga
     1621 cgaggccgat cttgcggccc gcggcacgca ggctgtcgac ggcgtcgcgg gccttggtgg
     1681 aaaacgaacc gatcatcacc agcaggtact cggcgtcgtc gctgcggtag caatcgagca
     1741 tctcgtagcg gcggccgaag acgctggcga attcgtcggc aatccggtcg taaatcggca
     1801 gggcttgccg cgccgcgaga tgcgcctggt agcggaaata ggaatagggc ccgccaccga
     1861 gcaccgcgat agcctggctc agcggtgcat tacccctgaa ttgcaggttt tccgggtcgt
     1921 agtcgccgac gaaagcccgc gccttttgtg cctcgggcag ttcgaccggt tcacgcgtga
     1981 aggatagata aaagccatcg aggttgacga tcgccggcac gcgcacctcg gcgtgctcgg
     2041 cgagccggta ggcgatgaga ataaagtccg cgacttcctg gcaggtggcg caatgaatct
     2101 gcagaaatcc gctgtcgcgc gcggccatga tatcgtt
//
LOCUS       DATCZV010000445         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_724987, whole genome shotgun sequence.
ACCESSION   DATCZV010000445 DATCZV010000000
VERSION     DATCZV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724987"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VIW27_10020"
     CDS             <1..702
                     /locus_tag="VIW27_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740051.1"
                     /translation="TEIVFGGVQDDKDGSLYPQQNLELEFETEGATVRELAANLDGYL
                     QLTGGGGRMKNSLALDLFGNIYAELLSTINPLVEREPYTTISCFAAYAEITDGVAEIE
                     PGAVLQTDKLNIFARGSIALDTEKIQLRFDTSPRKGLGISLGDFVNPFVGVSGTLASP
                     GLGVDPKNAMFQGGFAYATGGLSIVAKSLFDRWFGAEDPCAYLENEARESRREKHLPK
                     QQGAGERPASTVENR"
     gene            complement(712..1722)
                     /locus_tag="VIW27_10025"
     CDS             complement(712..1722)
                     /locus_tag="VIW27_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5740052.1"
                     /translation="MSTPYLDCSNEVTPVRQPGSDRLCWLAPEIGADDYLIEIPAAAR
                     DEIAALARYVEDNPLPVLYRRLEDFALPACRAMMAGLKRILDDGVGFAVLDRLPMDDF
                     DIDTLVQVYWLLGQSIGRPVAQKWDGQMIYHVTDTGAAYEYGTRGSYTSVELVFHTDN
                     AFARMVPDYVGLLCHNPAKQGGVSRFCSLYSVHQRMLELYPEQLVRLYQPMLFDRQKE
                     HREGDAPVCYAPYFSWRNNRLFARANSSLVRKGYQVAGESMDDALAAALDAIDEVCAA
                     EDLWYEAPLERGQIQYLNNHEVGHYRSAFEDYDDPERKRHLYRLWHREAGSTAYDGYS
                     QS"
     gene            complement(1772..2830)
                     /locus_tag="VIW27_10030"
     CDS             complement(1772..2830)
                     /locus_tag="VIW27_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY5740053.1"
                     /translation="MTLLLVYLLLAIGVSFICSVLEAVLLSISPGYVESLSMERPRAG
                     GAVSRVKQRIDESLAAILILNTFAHTMGAFGAGSQAVLVFGVRWETLVAVLLTLAILY
                     FSEIIPKTLGATFWKPLAIPAAYTILWLVKLVYPLVWISTRLTKLFGRHRESEITRDE
                     ILALAALGHREGALVSQENQYLYNLLRLREVTTEEVLTPRTVVHMLDQALSVSEALDN
                     PLTRQFSRIPVYGENLENITGKVLRHDLLMAERSGHGKAALSEFAKPIFRVSEKLPVQ
                     QLLDLFIKQKAHLFLVEDEFGQNSGIVTLEDAIETLLGREIVDESDTVVDMQELARGK
                     YRERLRAKRQDSGPDSAS"
     gene            complement(2944..3363)
                     /locus_tag="VIW27_10035"
     CDS             complement(2944..3363)
                     /locus_tag="VIW27_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020022698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY5740054.1"
                     /translation="MATELEFKCHVGVGPEVSGEALVTDDNFSARYDLDRIKGIFSRP
                     QHKLAGLSYVDKIMVFNTAKGGVASAWMLYEMKSRGIAPRALLFNSVNPILAQGAALA
                     GMALCDRFADGDVTRLIRTGDRVTVNPAAGRVTVIRD"
     gene            complement(3363..>3834)
                     /locus_tag="VIW27_10040"
     CDS             complement(3363..>3834)
                     /locus_tag="VIW27_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEY5740055.1"
                     /translation="PTLEAVCDPAALEAEVIGEADFEAFFALYGGKGEKVDVVVFSAP
                     QLSLLEMRQLAELLDGKKIHAATALLAVTSPVVAADARRLGLVERIESSGGLVLEGMC
                     FYQSYAREMGEANGWQRLLTNSVKLANIIGGYGYQPTLASMENCVASAIAGEIV"
BASE COUNT          718 a         1196 c         1211 g          709 t
ORIGIN      
        1 acggaaatcg tcttcggcgg ggtgcaggac gacaaggatg gatccttgta tccgcaacag
       61 aatctcgaac tcgaattcga aaccgagggc gcgacggtac gcgaactggc cgcgaacctc
      121 gacggttacc tgcagctcac cggcgggggc gggcgcatga aaaacagcct tgcgctcgac
      181 ttgttcggca atatttacgc ggaactgctc agcacgatca acccgctcgt cgagcgtgag
      241 ccgtacacga cgatttcctg cttcgccgcc tacgccgaaa ttaccgacgg cgtcgcggag
      301 atcgagcccg gcgcggtctt gcagaccgac aaattgaata tttttgcgcg cggcagcatc
      361 gctctcgaca cggaaaagat ccagctgcgt ttcgatacct cgccgcgcaa gggtctcggt
      421 atcagcctgg gcgacttcgt caatccgttc gtcggtgtaa gcggtacgct ggccagcccg
      481 ggcctcggcg tcgatccgaa aaacgcgatg ttccagggag ggtttgccta tgccaccggc
      541 gggctgtcga ttgttgccaa gagtctcttc gatcgctggt tcggcgccga ggacccctgc
      601 gcctacctcg agaatgaagc acgtgaatcc cggcgcgaaa aacaccttcc gaaacaacag
      661 ggcgctggtg aacggccggc ctcgacggtc gaaaatcgtt gaacggggcc tttagctctg
      721 ggagtagccg tcgtaagcgg tcgaaccggc ttcgcgatgc cacaggcggt aaaggtggcg
      781 cttgcgctcg gggtcgtcgt aatcctcgaa ggcgctgcgg taatggccga cctcgtgatt
      841 gttcaggtac tgaatctggc cgcgctcgag cggcgcttcg taccacaggt cttcggccgc
      901 gcaaacctcg tcgatggcgt cgagcgctgc agcgagggcg tcgtccatcg attcgccggc
      961 gacctggtaa cccttgcgca ccagcgacga attggcgcgc gcgaacaggc gattgttgcg
     1021 ccagctgaaa tagggcgcat agcagaccgg cgcgtctccc tcacgatgct ctttctggcg
     1081 gtcgaacagc atcggctggt agaggcgcac gagttgttcc gggtagagtt cgagcatccg
     1141 ctgatgcacc gagtagaggc tgcaaaagcg gctgacaccg ccttgtttcg ccgggttgtg
     1201 gcacagcagg ccgacatagt cgggcaccat gcgcgcgaac gcattgtcgg tatggaatac
     1261 cagctcgacc gaggtatagg agccgcgcgt gccgtattcg taggcggcgc cggtgtcggt
     1321 gacatggtag atcatttgcc cgtcccactt ctgcgccacc ggccggccga tgctttgccc
     1381 cagcagccag tagacctgca ccagcgtatc gatgtcgaag tcgtccatcg gcaggcggtc
     1441 gagcaccgcg aagccgacac cgtcgtcgag gattcgtttc aggccggcca tcattgcgcg
     1501 gcaggccggc agcgcaaaat cttccaggcg gcggtacagc accggcagcg ggttgtcctc
     1561 gacatatcgc gccagcgcgg cgatctcgtc gcgcgctgcc gccggtattt cgatcaggta
     1621 atcgtcggcg ccgatctccg gcgccagcca gcacaggcga tcgctgccgg gctggcgtac
     1681 cggtgtaacc tcgttgctgc aatccagata aggtgttgac atagactctg gccgaagttg
     1741 tttgcgcgaa ttataaatcc ccgcgacctg ttcacgaagc cgaatcgggg ccgctgtcct
     1801 ggcgcttggc gcgcaggcgt tcgcggtact tgcccctggc cagttcctgc atatcgacga
     1861 cggtgtcgct ctcgtcgacg atttcgcgtc ccagcagggt ttcgatcgca tcctcgaggg
     1921 tgacgatgcc cgaattctgg ccgaactcgt cctcgaccag gaacaggtgc gccttttgct
     1981 tgatgaacag gtcgagcaat tgctgcaccg gcaatttctc ggagacgcgg aagatcggct
     2041 tggcgaattc gctgagcgca gccttgccgt gtcccgagcg ctcggccatc agcaggtcgt
     2101 gccgcaggac cttgccggtg atgttctcga ggttttctcc atataccggg atacgcgaaa
     2161 actgccgtgt cagcggattg tcgagcgcct cgctgaccga cagcgcctgg tccagcatgt
     2221 gcaccacggt acgcggcgtc agcacttcct cggtggttac ctcgcgcagt ctcagcaggt
     2281 tgtacaggta ctggttctcc tgcgatacca gcgcaccttc gcggtgaccg agagcggcca
     2341 gcgcgaggat ttcgtcgcgc gtgatttcgc tttcccggtg gcgcccgaac agtttcgtca
     2401 ggcgggtcga gatccacacc agcgggtaaa ccagctttac cagccagagg atcgtgtaag
     2461 ccgccggaat cgccagtggc ttccagaaag tcgcgcccag cgtcttcgga atgatctccg
     2521 aaaaatacag gatcgccagc gtcaacagta ccgccaccag ggtttcccag cggacaccga
     2581 agaccagtac tgcctggctg cccgcgccga aggcccccat ggtatgcgcg aaggtattca
     2641 gaatcaggat cgcggcgagg gattcgtcga tgcgctgctt gacgcgcgac accgcgccgc
     2701 cggcgcgcgg cctttccatt gacaggctct cgacgtagcc gggggagatc gacagcagca
     2761 cggcctcgag taccgagcag atgaaggaaa cgccgatcgc cagcagcagg tagacgagta
     2821 gcagggtcat tgcgaaccag ccgcctgcgc agcggcgagt cgacggccgc ctgccgcgag
     2881 tcgaggatct cgcaggctgg tcgccgcatt acggtcgacg gcaggcggac gatggatcat
     2941 cgctcaatcc cgaatgacgg tgacccggcc ggcggcgggg ttgacggtga cccggtcgcc
     3001 ggtcctgatc aaccgcgtga cgtcgccgtc ggcaaagcgg tcgcacagcg ccattccggc
     3061 gagcgcggcg ccctgcgcga ggatcggatt gacggaattg aacagcagtg cacggggcgc
     3121 gatgccgcgc gacttcattt cgtagagcat ccacgccgat gcgacgccgc ccttggcggt
     3181 attgaaaacc ataatcttgt cgacgtaact taagcccgcg agcttgtgct gcgggcgcga
     3241 aaaaattccc ttgatgcggt cgaggtcgta gcgcgccgaa aaattgtcgt cggtgaccag
     3301 cgcctcgcca ctgacctcgg gaccgacgcc gacgtggcat ttaaactcga gctcagtggc
     3361 catcagacga tttccccggc gatcgccgag gcgacgcagt tttccatgct tgccagcgtc
     3421 ggctggtagc cgtaaccgcc gatgatgttg gcgagcttga ccgagttggt cagcaatcgt
     3481 tgccagccat tggcctcgcc catttctcgc gcgtagctct ggtagaaaca catgccctcg
     3541 aggaccagcc caccagagga ttcgatgcgt tcgaccagac ccaggcggcg cgcgtccgcc
     3601 gccaccaccg ggctggttac cgccagcagc gcggtagccg cgtggatttt cttgccgtcg
     3661 agcaactccg cgagttggcg catctcgagc agcgacaatt gcggcgccga aaacaccacc
     3721 acgtcgacct tctcgccctt gccgccgtag agcgcgaaga aggcctcgaa gtcggcttcg
     3781 ccgatgacct cggcctcgag ggccgcggga tcgcagacgg cttccagcgt gggc
//
LOCUS       DATCZV010000446         3721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_301069, whole genome shotgun sequence.
ACCESSION   DATCZV010000446 DATCZV010000000
VERSION     DATCZV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301069"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VIW27_10045"
     CDS             complement(<1..786)
                     /locus_tag="VIW27_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEY5740056.1"
                     /translation="MRLSKSQYVRGLQCHKALWLYKHKRELMTPTSPQQQFIFAGGHR
                     IGKLAQGLFPGGIEIEHDPQNFDGMIEQTRQLIERGATVIYEATFKSHDVLIMADILV
                     RNGAAWDLYEVKASTGVKPQHKPDAAIQWYVIRDHLPLGRAHIVHVDSNYVFDAELDI
                     SQLLAIEDITDGVLELQAELGKNLASMDDMLRHEEPDIAIGLQCENPYECDFKAYCWQ
                     DVPSPSVFDLYRMDKRRKFELYHAGKTGYAEVRGERLTRVQRLQ"
     gene            complement(818..1984)
                     /locus_tag="VIW27_10050"
     CDS             complement(818..1984)
                     /locus_tag="VIW27_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY5740057.1"
                     /translation="MTNPDPANQTATSRFGFGTQIRKSPFFEATVRWGATGFSVYNHM
                     YIPRDFGDPVGNFWNLVNSATLADVAVERQVEITGPDAARFTQLLTPRNLSQCAVSQC
                     RYVLITNAAGGILNDPVLLRLGENHFWLSLSDSDILLWAQGVAVHSGMDVEIREPDVS
                     PLQLQGPNSGDIMQALFGERIDRLRYYWLDEFELDGIPLVVSRTGWSSELGYEIYLRD
                     GSRGDELWETIMAAGKPHGLAPGHTSSIRRIEAGMLSYHADMDIDTNPFELGLERLVD
                     LQMEANFIGKAALTRIAREGVSRRQVGLEIDGAPLAGPNTECWPIKLAGERVGRVTSA
                     VYSPRLEANIALAMIRIENTTVDTRVEIETPAGSRAAKIVPKPFYDPAKNLPKR"
     gene            2350..3258
                     /locus_tag="VIW27_10055"
     CDS             2350..3258
                     /locus_tag="VIW27_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5740058.1"
                     /translation="MSFETAGIVLPVWLPLLWGVLVGLVFSTVGAAGGILASFGLISV
                     MGIQDPNLVKPMAQALTLATPLIAVPGYYRQGRMVFSLALILGAGGILGALVGSTFSA
                     AYLTDIDVFKSIFGVLTLGIALQVLSRLFTHAHKPATRSARAAAAFEGLVHDGGSTRT
                     IGVSHRHYSLQRIRFVFAGVQFEYPPWAPFLAGAGIAVVSSALGVGGGFLLVPFMVLL
                     LGLPMFLVAGTAALAIGVSSITSIGNYMRLGVELDMPLLLLLLAGTVAGAWLGPRLSR
                     HLHERWLEGILGLVLLLIGVRYLGSF"
     gene            complement(3343..>3721)
                     /locus_tag="VIW27_10060"
     CDS             complement(3343..>3721)
                     /locus_tag="VIW27_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5740059.1"
                     /translation="DNGYGRIVNLASTAGIRGGSGRTAYGAAKGGVIALTRVMAVELA
                     AYGITANALAPGAIETELVARMHSAETRRVYRAGIPLDRYGTPDETAFCAVFLASEQA
                     GYITGHILGIDGGFLAAGIMHKS"
BASE COUNT          583 a         1112 c         1237 g          789 t
ORIGIN      
        1 ctgcaatcgc tgcactctcg tcagccgctc gccacgaacc tccgcgtaac cggtcttgcc
       61 cgcgtggtag agctcgaact tgcggcgctt gtccatgcgg tacaggtcga agacggaagg
      121 cgagggtacg tcttgccagc agtatgcctt gaagtcgcat tcataggggt tttcgcattg
      181 caggccgatc gcgatgtcgg gctcctcgtg gcgcagcatg tcgtccatcg aagccaggtt
      241 tttcccgagt tccgcctgca actcgagcac cccgtcggta atgtcctcga tggccagcaa
      301 ctggctgata tcgagctcgg cgtcgaacac gtagttgctg tcgacgtgca cgatgtgggc
      361 cctgccgagc ggcaggtggt cacggatcac gtaccactgg atggcggcgt cgggcttgtg
      421 ttgcggtttg acgccggtcg aggccttgac ctcgtagaga tcccacgcgg cgccgttgcg
      481 taccaggatg tctgccatga tcaaaacgtc gtggctcttg aaggtggcct cgtaaatgac
      541 cgtcgcgccc cgctcgatca gttgccgggt ttgctcgatc atgccgtcga agttttgtgg
      601 gtcgtgttcg atctcgatgc cgcccgggaa caggccttgc gcgagtttgc cgatgcggtg
      661 gccgcccgcg aaaatgaatt gctgctgcgg gctggtgggt gtcatcagct cgcgcttgtg
      721 tttatacagc cacagcgcct tgtggcattg caggccgcgg acgtattgtg atttcgacag
      781 ccgcatgtct gggtttccgg gttcgagccg ctactcgcta acgcttcggc aggtttttcg
      841 ccgggtcgta gaagggtttg ggcacgatct tcgcggcccg gctgccggct ggcgtctcga
      901 tctcgacgcg agtgtcgaca gtggtgtttt cgatgcggat catcgccagc gcgatgttgg
      961 cctcgaggcg cggggagtaa acggcggagg tcacccgccc gacgcgttca ccggccagct
     1021 tgatcggcca gcactcggtg ttcggtcccg ctaacggcgc accgtcgatt tcgaggccca
     1081 cctgccggcg gctgacgccc tcgcgcgcga tgcgggtcag ggccgccttg ccgatgaagt
     1141 tggcctccat ttgcaggtcg accaggcgct ccagcccaag ctcgaaggga ttggtgtcga
     1201 tatccatatc agcgtggtag gacagcatcc cggcctcgat gcggcgaatc gaagaggtat
     1261 gcccgggggc gagcccgtgc ggcttgccgg cggccatgat cgtctcccac agttcgtcgc
     1321 cgcggctgcc gtcgcgcagg tagatttcat aacccagttc gctggaccag ccggtgcgag
     1381 agacgactag cggaatgccg tcgagctcga actcgtccag ccagtagtag cgcagccggt
     1441 cgatccgctc gccgaacagg gcttgcatga tatccccgga attcggcccc tgcagttgca
     1501 acggtgaaac atcgggttcg cgaatctcga cgtccatgcc gctgtgtacg gcgacgccct
     1561 gcgcccacag caggatatcg ctgtcggaca gcgacagcca gaaatggttt tcacccagcc
     1621 gcagcagcac cgggtcgttc agtatgccgc cggcggcgtt ggtgatcagc acgtatctgc
     1681 actggctgac ggcgcattgc gacaggttgc gcggcgtcag caactgggtg aagcgcgcgg
     1741 cgtcggggcc ggtaatctcg acctgccgct caaccgcgac gtcggcgagc gtggcgctgt
     1801 tgacgaggtt ccagaaatta cccaccgggt cgccgaaatc tctcgggatg tacatgtggt
     1861 tgtagaccga aaagccggtc gccccccagc gtaccgtggc ctcgaagaac ggagattttc
     1921 gaatctgcgt accgaagccg aagcggctgg tggcggtttg gttggccggg tcggggttag
     1981 tcattgtcac tcggataaat gattgctggc gcctggctgc gaaatgccgg gaatacgtcg
     2041 actattgcgc cggcagtata cttgcattgc cccggaattt cgctgctggc tcgaaaatcc
     2101 gaaaaccgtt tgctggcctg cgcgagcggc cctgttgctc gccgcgctcg ccgtgtattc
     2161 gccgcaccgg acggcactct gccagtcatg cccgcgcaag cggcatctcg agatgcttgc
     2221 gtggtagcag ggtattcccg ctggggatgc gggcggctgg tacctaaacg acgaaagggc
     2281 cggcaatgac tcgaggagag tgggtatact gtcccggggt ttgtcgatat cagcaggctt
     2341 ctggtgtcaa tgtcttttga aactgcaggg atagtcctgc cggtctggct gccgttgcta
     2401 tggggcgtgc tggtggggct ggtgttttcc acggttggcg ccgccggcgg tatcctggct
     2461 tccttcggcc tgatttcggt aatggggata caggatccga atctggtcaa gccgatggcg
     2521 caggccctga ccctggcaac gccgctgatc gcggtgccgg gatactaccg ccagggccgg
     2581 atggtgttca gcctggcttt gatcctcggc gccggcggca tcctcggcgc tcttgtcggc
     2641 agtacctttt cggctgccta tctcaccgat atcgacgtgt tcaagtcgat cttcggcgtg
     2701 ctcacgctgg ggatcgcgtt gcaggttttg tcgcggttgt tcacgcatgc gcataagcct
     2761 gccacgcgct ccgcgcgcgc cgccgccgcc ttcgagggac tcgtacacga tggcgggtcg
     2821 acccgcacca tcggcgtcag ccatcggcac tactcgttac agcgcattcg cttcgtattc
     2881 gccggcgtgc aattcgaata cccgccctgg gcgccgtttc tggccggcgc ggggattgcc
     2941 gtggtctctt cggcgctggg ggtcggtggc ggtttcctgc tggtgccgtt catggtgctg
     3001 ctgctgggct tgccgatgtt cctcgtcgcc ggcaccgcgg ccctggcgat tggggtgagt
     3061 tcgataactt cgatcggtaa ctacatgcgg ctgggtgtcg aactcgacat gccgctgttg
     3121 ttgttgctgc tggcgggcac ggttgcggga gcctggctcg ggccgcgcct cagcaggcac
     3181 ctgcacgagc gatggctgga gggaatcctg ggtttggtgc tgttgctgat cggggtgcgc
     3241 tacctgggca gtttttagtg ttcgaataat cgggcgcaag ggaaatgcgg gatgagcgcc
     3301 acccgcgcgc cttcctgcgc atctcgaata gccgcggcca actcaggatt tatgcatgat
     3361 cccggcggcg aggaaaccgc catcgatacc tagaatatga cccgtgatat agcctgcctg
     3421 ctcgctggcc agaaataccg cgcaaaacgc ggtttcatcc ggtgtgccgt agcgatccag
     3481 cggaatgccg gcgcggtaga cgcggcgtgt ctccgcggaa tgcatgcgcg ccaccagctc
     3541 ggtttcgatg gcgccgggag ccagcgcatt ggcggtgatc ccgtaagccg ccagttcgac
     3601 ggccatcacc cgggtcaggg cgatgacccc gcccttggcg gcgccatagg cggtgcgccc
     3661 gctgcctccg cggatgccgg cggtggaggc gagattgacg atgcgtccat agccgttgtc
     3721 g
//
LOCUS       DATCZV010000447         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_89454, whole genome shotgun sequence.
ACCESSION   DATCZV010000447 DATCZV010000000
VERSION     DATCZV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89454"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1164
                     /gene="asd"
                     /locus_tag="VIW27_10065"
     CDS             49..1164
                     /gene="asd"
                     /locus_tag="VIW27_10065"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5740060.1"
                     /translation="MKTVGFVGWRGMVGSVLMQRMLEERDFDRIDEPVFFSTSQQGQP
                     GPDIGREVPPLRDAQDIAQLRAMDAIVSCQGGDYTKQVYADLRGSGWQGYWIDAASAL
                     RMESDSIIVLDPVNHDVIERAIDRGCRDYIGGNCTVSLMMMAMGGLFEHDLVEWISVM
                     TYQAASGGGARHMRELIAGMGSLYRSVAEQLDDPAAAILDIDRQVTEMLRSADFPRRE
                     FGVPLAGSLIPWIDAQLDNGQSREEWKGGVEANKILGREQNMIPIDGLCVRVGAMRCH
                     SQALTVKLRRDLPLDEIHAMLAAANDWVRVLPNDKAVTIEALSPAAVTGTLDIPVGRL
                     RKLGMGAEYLAAFTVGDQLLWGAAEPLRRILNILQQH"
     gene            complement(1385..1597)
                     /locus_tag="VIW27_10070"
     CDS             complement(1385..1597)
                     /locus_tag="VIW27_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740061.1"
                     /translation="MTAVREFTLKKALSQADVNIDYRKDFSKRSETFIILMTCAQLAH
                     SITRATARDDGAHGAGCNRLIAQLNS"
     gene            1659..>3315
                     /locus_tag="VIW27_10075"
     CDS             1659..>3315
                     /locus_tag="VIW27_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY5740062.1"
                     /translation="MRNLGLIVAIWLAAIVPTQALALGLGEIEVKSFLNQPLEAEIEV
                     ISARPGEIDDLLVSLASREAFARAGLSRPRHLLDMRFAVNKSEDGSEAVILVTTKDGV
                     KEPFLNFLIEADWAKGRVLREFTILLDPPFYAEQPPPVEPPSQPAATSPIADDTSVGD
                     IAQQMGVTETTMPEPEASDSGQQFSEPIALAEDSAPQQAVVEQPVEQPPAQPVEESSS
                     FQFVLDTPQQNLADEVLVSKGDTLWSIASRYRDGSRSMSQIMLAFQRANPAAFNDGNI
                     NNMREGAILRIPDSAELDALDAQQAYAEVLVQNGLWDEYVARVTGVSPVAAVDQREAV
                     EVTGSVSVSDEPSGELSLLMPADGSGDSAGTGDGSELGELRNRLAMAEEELEASRIEN
                     RELESRIAELQARLSKMEELQKMVEIEDDSLAQLQADSAAEQSSAAASQQPEGTFSGD
                     DDVSEQALIEELLAEEAAAQAEEQATTMAANDGMVSDEVAMAEPMTDPNSASAGDSMA
                     EDQAAPEAGGTVGETQANQQSTPPAPVIITETAVREPSIFDGIL"
BASE COUNT          653 a          989 c         1074 g          599 t
ORIGIN      
        1 gattacattt cgacttttgt taacaagttt tttccagaat caaagaaaat gaagactgta
       61 ggtttcgtcg gctggcgcgg catggtcggc tcggtattga tgcagcgcat gctcgaagag
      121 cgcgatttcg atcgtatcga cgagccggta ttcttttcga cctcgcagca gggacagccg
      181 ggtcccgaca tcggcaggga agtgccgccg ttgcgggatg cgcaggatat cgcgcagctg
      241 cgagcgatgg atgcgatcgt cagctgccag ggcggcgatt acacgaaaca ggtttacgcc
      301 gatctgcgcg gcagtggctg gcaggggtac tggatcgatg cggcctcggc actgcgcatg
      361 gagagcgaca gtatcatcgt gctcgatccg gtcaaccacg acgtcatcga gcgcgcgatc
      421 gaccgcggtt gccgcgatta catcggcggc aattgcaccg tcagcctgat gatgatggcg
      481 atgggcggac tgttcgagca cgacctggtc gagtggatca gcgtgatgac ctaccaggcc
      541 gcctccggcg gcggtgcgcg tcacatgcgc gaactgatcg ccggcatggg cagcctgtac
      601 cgcagcgtag ccgagcagct cgacgacccg gcggcggcga ttctcgatat cgaccggcag
      661 gtcaccgaga tgctgcgctc ggcggacttt ccgcgccgcg agttcggcgt accgctggcc
      721 ggcagcctga ttccgtggat cgacgcgcag ctcgacaacg gccagagccg cgaagagtgg
      781 aagggcggtg tcgaggccaa caagatactt ggccgcgagc aaaacatgat tccgatcgac
      841 gggctgtgcg tgcgcgtcgg cgcgatgcgc tgtcactcgc aggcgctgac ggtcaagctc
      901 aggcgtgacc tgccgctcga cgagattcac gcaatgctcg ccgccgccaa cgactgggtg
      961 cgagtgctgc ccaacgacaa ggcggtaacc atcgaagccc tgtcgccggc ggcggtcacc
     1021 ggcacgctcg acattccggt cggccgcctg cgcaagctcg gcatgggtgc cgaatacctg
     1081 gcggcgttca cggtcggcga tcagctgctg tggggcgcgg ccgaaccgct gcggcgtatt
     1141 ctgaacattc tgcagcagca ctgagcggcg cgcagcgctg tccctatcac cgcgtcgagc
     1201 aggctgcgag ccttggcgaa ttttgggaaa tagttcatat tcttcgcggc gcgccgcgcc
     1261 gccatggccg actcgttgcc gggaagtccg gcgcgcggcg ttcgccgcct gccagacggt
     1321 tgcccggagc cgcttttgcc ggcttttgca gaaccgctcg acattcccgg ctttccgatt
     1381 ggaatcaact gtttagctgt gctataagcc tgttgcaacc agctccatgg gcgccatcat
     1441 cgcgcgccgt ggcgcgggta atcgaatgcg ccagctgcgc acatgtcatt aaaataatga
     1501 atgtttcaga tcttttgcta aaatcttttc tgtaatctat atttacatct gcttgtgaaa
     1561 gtgctttctt taaagtaaat tctctgactg cggtcatagg tgcatggaat ttatagcact
     1621 tttaagaaga ataagtcggg gagctgggag actaaaacgt gcgtaatctg ggactcatag
     1681 tagcaatttg gctggcggca atcgtgccga cccaggcgct cgccctgggc ctgggcgaga
     1741 tcgaggtcaa gtcgttcctg aaccagccgc tggaagcaga aattgaagtc atttccgcgc
     1801 gtcccggcga gattgacgac ctgctggtca gccttgcttc gcgcgaggcg tttgcccgcg
     1861 ctggcctgtc ccgcccccgg cacctgttgg acatgcgctt cgcggtaaac aagagcgaag
     1921 acggcagcga ggccgtcata ctcgtcacca ccaaggatgg cgtcaaggaa ccgttcctga
     1981 atttcctgat cgaggccgat tgggccaagg gcagggtatt gcgcgagttc acgatcctgc
     2041 tcgacccgcc attttatgcc gaacagccgc cgccggtaga accccccagt cagccggccg
     2101 ccacgtcgcc gattgccgat gacacgagcg tcggcgatat cgcccagcaa atgggcgtca
     2161 ccgagaccac gatgcccgaa cccgaggcat cagacagcgg acagcagttc agcgaaccga
     2221 tcgcgcttgc cgaagatagt gcgccgcaac aggccgtggt cgagcagcca gtcgagcagc
     2281 ccccggccca acccgtcgag gagtcgagca gtttccagtt cgttctcgat actcctcagc
     2341 aaaacctcgc cgacgaggtt ttggtctcga agggagatac tctgtggagt attgccagcc
     2401 gctatcggga tggtagccgc agcatgtcgc agatcatgct cgcatttcag cgcgccaatc
     2461 ccgccgcctt caacgacggc aacatcaaca acatgcggga gggcgcgatc ctgcgcattc
     2521 ccgattccgc cgagctggac gcgcttgacg cgcagcaggc ttacgccgag gtgctggttc
     2581 aaaacggcct gtgggacgaa tacgtcgctc gcgtgaccgg cgtgtcgccg gtagccgccg
     2641 tcgaccagcg cgaggccgtc gaggtcacgg gctcggtgtc cgtcagcgat gagcccagcg
     2701 gcgaactgag cctgctgatg cccgcggacg gcagcggcga cagcgccggc accggtgacg
     2761 gcagtgaact cggcgagttg cgcaaccggc tggcgatggc cgaggaagaa ctcgaggctt
     2821 cgcgaatcga gaaccgcgaa ctcgaatccc gtattgccga gttgcaggct cgattgtcga
     2881 agatggaaga gctgcaaaag atggtcgaga tcgaagacga cagtctcgcg cagttgcagg
     2941 ccgattcggc ggcggagcaa tcttcggcgg cagcctcgca acagcccgag gggaccttct
     3001 ccggggacga cgatgtcagc gagcaggcgc tgatcgagga attgcttgcc gaggaagccg
     3061 cggcgcaggc cgaggagcag gcgacgacga tggcggccaa cgacggcatg gtcagtgacg
     3121 aggttgcgat ggcggagccg atgacggatc cgaacagcgc gagcgcgggc gactcgatgg
     3181 ccgaagatca ggcggcgccc gaggcgggcg ggaccgtcgg tgaaacccag gcgaatcagc
     3241 agtcgacgcc gccggcgccg gtcatcatca ccgaaacggc agtccgcgaa ccttcgattt
     3301 tcgacggcat tctgc
//
LOCUS       DATCZV010000448         2711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_619667, whole genome shotgun sequence.
ACCESSION   DATCZV010000448 DATCZV010000000
VERSION     DATCZV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619667"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /gene="murD"
                     /locus_tag="VIW27_10080"
     CDS             <1..982
                     /gene="murD"
                     /locus_tag="VIW27_10080"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY5740063.1"
                     /translation="GSNGKSTVTRLVGDMVARDGHRVAVGGNLGTPALDLLDSGAQTY
                     VLELSSYQLETTSSLRPRVAALLNLCEDHLDRYDSYADYIDAKRRVFRHAEICVCNLD
                     DPATRHAGDDVTFSLDASSSADFRLVERHGLQLAHGDDAWIGIDELRVGGRHNWANCL
                     AAMAIAHGLGVSRQAAIEAMRDFEGIPHRSQWVAEIDGVEWINDSKATNVGAAQASID
                     GRDRPVILIAGGQSKGADLSVMTDSLRARVKLALLLGEDAERLQQAWQGATRIERVQD
                     MAEAVRRAREFASPGDCVLLAPACASFDMYEKFEARGDDFMRRVRELDNG"
     gene            975..2174
                     /gene="ftsW"
                     /locus_tag="VIW27_10085"
     CDS             975..2174
                     /gene="ftsW"
                     /locus_tag="VIW27_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY5740064.1"
                     /translation="MASQAIRTAGQARQQQDEFAAGFDRTILLLYVALVGIGLVMVTS
                     ASISIADQQTGDPFFYTKRQFLRVLLSLALLWFACRIPLEFWKNNGMALMLGSIVLLA
                     CVLIPGVGHTVNGSTRWLNFGLFTFQVSEIAKLFLIIYLSGYLIRRSDEIKTNTMGFI
                     KPMLILAVASGLLVMEPDFGAAAVLLMTGLGLIFLGGVRFGQFVLFVSGTLVIMVLLA
                     ISSPYRLARITSFMDPWADPFNSGFQLTQSLIAIGSGGWFGAGLGGSIQKLFYLPEAH
                     TDFMFAIFAEEFGFAGQLVLIGLFALFALRCFAIARLVMLREQAFAAYLAYGVGLLIT
                     LQAIINIGVNMGALPTKGLTLPFISYGGNSILSMSFAVGLILRVCYEYRLVDGATPAP
                     RRRGKGS"
     gene            2171..>2711
                     /locus_tag="VIW27_10090"
     CDS             2171..>2711
                     /locus_tag="VIW27_10090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5740065.1"
                     /translation="MSEALQRPILVMAGGTGGHVFPALAVAERLRERGENIVWLGTHS
                     GIEARVVPAADFAIEWLSVQGLRGKGLASLMLAPLRLLRACWQALGILRRTRPKAVLG
                     MGGFVSGPGGLMAWLLHIPLFVHEQNSVIGLTNRILSRLAVRSYFAFPQAAAKITGGE
                     CIGNPVRADFAGFDDPARRL"
BASE COUNT          425 a          832 c          913 g          541 t
ORIGIN      
        1 cggctcgaat ggcaagagca cggtaacccg gctggtcggg gatatggtcg cccgcgacgg
       61 ccacagggtg gcggtcggcg gcaatctcgg caccccggca ctggacctgc tcgacagcgg
      121 cgcacagacc tacgtgctcg aactgtccag ttatcagctc gagaccacca gttcgctgcg
      181 gccgcgtgtc gccgcgctgc tgaatctgtg cgaagaccat ctcgatcgtt acgacagtta
      241 cgcggattac atcgacgcca agcggcgcgt cttccggcat gcggaaatct gtgtctgcaa
      301 tctcgacgac ccggccacgc gccatgccgg cgacgatgtg acctttagtc tcgacgcgtc
      361 atcgagcgct gatttcaggc tcgtcgaacg gcacggactg cagctcgcgc atggcgacga
      421 tgcatggatt ggcatcgacg aactgcgggt cggcggccgc cataactggg ccaactgcct
      481 ggcggcgatg gcgatcgcgc acggcctcgg tgtctccagg caggcggcga tcgaggcgat
      541 gcgcgatttc gaaggtattc cgcatcgcag ccagtgggtg gcggaaatcg atggcgtcga
      601 atggatcaac gactccaagg cgaccaacgt cggcgccgcg caggcttcca tcgacggtcg
      661 cgatcgcccg gtgatcctga tcgccggcgg gcagtcgaag ggtgccgacc tgtcggtaat
      721 gaccgacagc ctgcgcgcca gggtcaagct ggcattattg ctgggcgagg acgccgagcg
      781 cctgcaacag gcctggcagg gggcgacgcg catcgagcgg gtgcaggaca tggccgaggc
      841 ggtgcggcgc gcacgggaat tcgccagccc gggcgactgc gtgttgctgg cgccggcctg
      901 tgcgagcttc gatatgtacg aaaagttcga ggcgcgcggc gatgacttca tgcggcgggt
      961 cagggagctc gacaatggct agccaggcaa tccgcacagc cggtcaagcc cgccagcagc
     1021 aggacgagtt cgcggccggc ttcgatcgta ccatcctgtt gctgtacgtg gcgctggtgg
     1081 gcatcgggct ggtgatggtg acctcggcgt cgatcagcat cgccgaccag cagaccgggg
     1141 atccgttttt ctacaccaag cgccagttcc tgcgcgtgtt gctgagcctc gcgctgctgt
     1201 ggttcgcgtg ccggattccg ctcgagttct ggaaaaataa cggcatggcg ctgatgctgg
     1261 gttcgatcgt gttgctcgcc tgcgtgctga ttccaggtgt cggtcatacc gtcaacggca
     1321 gtacccgctg gctcaacttc ggcctgttta cttttcaggt ctcggaaatt gccaagttgt
     1381 tcctgatcat ttacctgagc ggttacctga ttcgccgcag cgatgaaatc aaaaccaata
     1441 ccatgggctt catcaagccg atgctgatcc tcgcggtggc cagtggcctg ctggtcatgg
     1501 agccggattt cggcgccgcg gcggtgttgc tgatgaccgg cctcgggctg atttttctcg
     1561 gcggcgtgcg tttcgggcaa ttcgtactgt tcgtcagcgg gacgctggtg atcatggtgc
     1621 tgctggcgat ctcgtcgccg taccggctgg cgcgcatcac ctcgttcatg gatccctggg
     1681 cggatccgtt caacagcggt tttcagctga ctcaatcgct gatcgcgatc ggcagcggcg
     1741 gttggttcgg cgccggactc ggcggcagca tccagaagct gttttacctg ccggaggcgc
     1801 acacggactt catgttcgcg atcttcgccg aggaattcgg cttcgccggt cagctcgtac
     1861 tgatcggcct gttcgcgctg ttcgcgctgc gctgcttcgc catcgcgcgc ctggtgatgc
     1921 tgcgcgagca agccttcgcg gcatacctgg cctatggtgt cgggctgctg atcacgctgc
     1981 aggcgataat caatatcggc gtcaacatgg gggcgctacc gaccaagggg ctgaccctgc
     2041 cattcatcag ttacggtggc aactcgatcc tcagcatgtc gttcgcggtc gggctgatac
     2101 tgcgggtctg ctacgagtac cggctcgtcg acggtgccac gccggcgccc aggcgccggg
     2161 ggaaggggtc atgagcgagg cgctgcaaag acccatcctg gtgatggcgg gcggtaccgg
     2221 cggtcacgtg ttcccggcgc tggcggttgc cgagcgcctg cgcgagcgtg gcgaaaacat
     2281 cgtctggctc ggcactcact ccggtatcga ggcacgcgtg gtgccggccg ccgatttcgc
     2341 catcgagtgg ctcagcgtgc agggattgcg cggcaagggt ctggcctcgt tgatgctggc
     2401 gccgctgcgc ctgctgcgtg cctgctggca ggcgctcggc attctgcgcc gtacccggcc
     2461 gaaggcggtg ctcggcatgg gcggcttcgt ttccggtccc ggtggcctga tggcctggtt
     2521 gctccatatt ccgctgttcg tgcacgaaca aaactcggtc atcggcctga ccaaccgcat
     2581 tctgagccgg ctcgcggtgc gcagctactt cgcgttcccg caggccgcgg ccaaaatcac
     2641 gggcggtgaa tgcatcggca atccggtgcg cgccgatttt gccggcttcg atgatcccgc
     2701 gcggcgtctc g
//
LOCUS       DATCZV010000449         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_672326, whole genome shotgun sequence.
ACCESSION   DATCZV010000449 DATCZV010000000
VERSION     DATCZV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672326"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VIW27_10095"
     CDS             <1..1149
                     /locus_tag="VIW27_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY5740066.1"
                     /translation="VYAGRNGIIGALTEDLIDVSQESEETIAALRHTPGGAFGSCRYK
                     LKSLEENQAQYERLIEVFKAHDIGYFFYNGGNDSADTSYKVSQIAAKMGYPLVCIGVP
                     KTVDNDLAVTDCCPGFGSVAKYVATSIREAGFDVASMARTSTRVFVLEVMGRHAGWIT
                     AACGLAAEAEGDGPHILLFPEIPFDQERFLAKTDDSIRRYGYCTVGVSEGLRWPDGKF
                     LSESGLRDAFGHAQLGGVAPLIAQLVRDKLKQKYHWAVADYLQRSARHIASKTDTLQS
                     YAVGKAAVELAVKGRSGVMPVIVRKSARPYRWTIGVADLKDIANHEKMMPRDYITEDG
                     FHITEKCRQYLAPLVQGEDYPPYRNGLPAYVKLKNTPVPKKLAAAFKI"
     gene            1311..>3101
                     /locus_tag="VIW27_10100"
     CDS             1311..>3101
                     /locus_tag="VIW27_10100"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY5740067.1"
                     /translation="MSYDLLFAFICSLAGILMGAVWIKSILAHPAGNDRMREIAAAVQ
                     QGAVAYLTRQYKTIAIVGVALFVLIGVGLGSWSTAVGFLIGAVLSGAAGAIGMNVSVR
                     ANVRTAEAARNGLDAALALAFRGGAVTGMLVVGLGLLGVTVFYWVLLGSAAPGATIKD
                     IAHPLIGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFETYAVTIIATMLLGALLLSKPDVAPLAASYPLVLGGVSIIAS
                     IIGCYFVKAASGKKIMAALYRGLIVSGVLAAIGFVAVTYWMMGDISQQYPELTPLKLL
                     GAALVGLVLTGLMVVITEYYTGTDFAPVRHIASASTTGHATNIIAGLGVSMKATAWPV
                     IAVCASIYAAYTLAGLYGIAIAATAMLSMTGIIVALDAYGPITDNAGGIAEMADLPKE
                     VRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALESAGKAVRFDLSDPAV
                     LIGLFIGGLVPFLFGSMAMEAVGRAAGSVVVEVRRQFKEIPGIMEGTGKPDYSRAVDM
                     LTRSAIKEMIVPSLLPVLVPIVVALVMNWLM"
BASE COUNT          528 a          992 c          998 g          583 t
ORIGIN      
        1 gtgtacgccg ggcgcaacgg catcatcggc gccctcaccg aggacctgat cgacgtcagc
       61 caggagtcgg aagagacgat cgccgccctg cgccacaccc ccggcggggc cttcggctcg
      121 tgccgctaca aactgaaaag cctcgaggaa aaccaggccc agtacgagcg gctgatcgaa
      181 gtcttcaagg cgcacgacat cggctacttc ttctacaacg ggggcaacga ctcggcggac
      241 acgtcataca aggtctcaca gatcgccgcc aaaatgggct accccctggt ttgcatcggc
      301 gttcccaaga ccgtcgacaa cgatcttgcc gtgacggact gctgcccggg cttcggttcc
      361 gtggccaagt acgtcgcgac cagcatccgg gaggcgggat tcgacgtcgc gtcgatggcg
      421 cgcacatcaa cgcgcgtgtt cgtgctggag gtgatgggcc ggcatgccgg gtggatcacc
      481 gctgcctgcg gtctcgccgc ggaagcggag ggcgatgggc cccatatcct gctttttccc
      541 gagatcccgt tcgaccagga gcgcttcctc gcaaaaacgg atgacagcat tcggcggtat
      601 ggctactgca cggtcggcgt gtcggagggc ctgcgctggc ctgacgggaa atttctttcg
      661 gaatcgggcc tgcgcgatgc ttttggccac gcgcagctgg gcggcgtcgc ccccctcatc
      721 gcgcagctcg tccgcgacaa gctcaagcaa aaataccact gggccgtcgc agactacctg
      781 cagcgctccg ctcgccacat tgcctcgaaa accgacaccc tgcagtcgta cgcggtggga
      841 aaagctgccg tcgagctggc cgtgaagggg cgcagcgggg tgatgccggt catcgtcaga
      901 aagtcggccc ggccgtaccg ctggacgatc ggcgtggcgg acctgaagga tatcgccaac
      961 cacgaaaaaa tgatgccgcg cgactacatc accgaggacg ggtttcacat cacggagaag
     1021 tgtcggcagt atctcgcgcc gctggtgcag ggcgaggatt atccgccgta ccggaacgga
     1081 ctgccggcat acgtcaagct caagaacacg ccggttccga agaaactcgc ggccgcattc
     1141 aaaatctgaa cggctggcga acggtgaaca gatttgcggt ggcagtcctg gctgcttttt
     1201 ttgattaccg tcgaccggtt aacgattgcc gtttactgac aggcttgcac ccgcgttgat
     1261 ctgcattcat ccgcggctaa atacataacg ttccaatcaa ggggagtccc atgtcatacg
     1321 acctgttgtt cgcgttcatc tgctcgctcg cgggcatctt aatgggcgcg gtctggatca
     1381 agtcgatcct ggcccatcct gccggaaatg atcgcatgcg cgagatcgct gcggctgttc
     1441 agcagggcgc ggtcgcgtat ttgaccaggc agtacaagac catcgccatc gtcggcgtcg
     1501 cgctctttgt cctgatcggc gtcggcctcg gctcgtggtc cacggctgtc ggcttcctga
     1561 tcggcgcggt gctctcgggc gccgcgggcg cgattggaat gaacgtgtcg gtgcgcgcga
     1621 atgtgcggac ggccgaggcg gcgcgcaacg gactcgacgc ggcgctcgct ctcgcgttcc
     1681 ggggcggggc ggtcacgggc atgctcgtgg tcgggctagg gcttttgggc gtaacggtct
     1741 tttactgggt gctgctcggc agcgcagcgc ccggcgccac gatcaaggat atcgcgcacc
     1801 ccctcatcgg ccttgcgttc ggcggctccc tcatttcgat cttcgcccgg ctgggcggcg
     1861 gaattttcac caagggcgcg gacgtcggcg ccgacctggt cggcaaggtc gaggcgggca
     1921 ttcccgagga cgacccgcgt aacccggccg taatcgccga caacgtgggc gacaacgtcg
     1981 gcgattgcgc cggcatggcg gccgacctgt tcgagaccta cgcggtgacc atcatcgcga
     2041 ccatgctgct cggcgcgctc cttctgtcga aaccggacgt cgcgccgctg gccgcgtcct
     2101 acccgctggt cttgggcggc gtctcgatca tcgcgtcgat tatcggctgc tacttcgtca
     2161 aggcagcgag cggcaagaaa atcatggcgg ccctttaccg cggactcatc gtctcggggg
     2221 tgctcgcggc gatcggcttc gtcgcggtca cctactggat gatgggcgac atctcgcagc
     2281 aatatcccga gctgacgccc ctcaagctcc ttggggccgc gctggtcgga ctcgtgctca
     2341 ctggtctcat ggtggtcatt accgagtact acacgggcac cgatttcgct ccggtgcgcc
     2401 acatcgcgag cgcctcgacg accggtcacg cgaccaacat catcgcgggc ctcggggttt
     2461 cgatgaaagc gacggcgtgg ccggtgatcg ccgtgtgcgc gagcatttac gcggcctata
     2521 ccctggcggg cctctacggg atcgcgatcg cggcgacggc catgctttcc atgaccggca
     2581 tcatcgtggc gctcgacgcc tacggcccga ttaccgacaa tgcaggcggc atcgcggaaa
     2641 tggccgacct gcccaaggaa gtgcgggcga ttaccgaccc tctcgacgcg gtaggcaaca
     2701 cgaccaaggc cgtgaccaag ggttacgcga tcggctcggc agggctggca gcgctcgtgc
     2761 tctttgccga ctacacgcat gcgctcgagt cggccggcaa ggccgtgcgt ttcgaccttt
     2821 ccgatcccgc cgtcctgatc gggctcttca tcggcgggct tgtgcccttc cttttcggct
     2881 ccatggcgat ggaagccgtt ggccgcgccg ccggctcggt cgtggtcgag gtgcgccgcc
     2941 agttcaagga aatccccggc atcatggagg gaacgggcaa acccgattac tcccgcgccg
     3001 tagatatgct gacccgttcc gcgatcaagg aaatgatcgt gccgtcgctg ctgcccgtgc
     3061 tggtgccgat cgtcgtggcg ctggtcatga actggctgat g
//
LOCUS       DATCZV010000450         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_568065, whole genome shotgun sequence.
ACCESSION   DATCZV010000450 DATCZV010000000
VERSION     DATCZV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568065"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VIW27_10105"
     CDS             complement(<1..1412)
                     /locus_tag="VIW27_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740068.1"
                     /translation="MNLDKLQVNACTRSGWQAVDLGFLMARAWFRPLLAAGLLPMLPL
                     AAVLLLLFRETPFWALFIVWWLKPFWERLPLFIASRRLFDEEAGVWQSMKSFPLKDVL
                     PWLLWRRFSMQRAFDNPVTVLEELRGAQRRARLRVLHGKYSDVALGNQFVCFCFEVLL
                     AFGILVMLAFFTPDAFELAITDRYGDLTLVGEWMYTLAGIAAIGLVVPFHTMAGFALY
                     LNRRIELEAWDIEISFRNLAARKRAAAGGAGSLLASLLLALTLTAIVPAQVEAAQEHD
                     RESAAALIDEVLTGEDFGYQRTVRKWRFKNWNQAEAGEDEEIPQWLIDFFDWWETNLD
                     WSTDFSGAAAWLKLMLVLLFVGLLIFLIRRYRGPLSRLVSTRRRQAAPEILFGLDVTP
                     ASLPDDVPGQVMKLWNAGSHREALSLLYRASLSRLIDRYEIAFRASHTETECAALVTE
                     RGIDSLSEYFWQLTDVWRRLA"
     gene            complement(1399..2355)
                     /locus_tag="VIW27_10110"
     CDS             complement(1399..2355)
                     /locus_tag="VIW27_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY5740069.1"
                     /translation="MKQHSFEAQHEETWARIEASLQQPSKGNNSRVLAEHYMLLCQHL
                     AIAKERLYDAALVERLNNLVLAVYRELYRYRSETRLNLFAFLRRDFPLAIYRQRYFVL
                     AALLVFLLPGLLAGAWTYFDDEALYSMVDAHQVRQVERMYDPEARVLGREREADTDIY
                     MFGFYIKNNISVAFRCFAGGILVGIGTLLVLFFNGMFIGGIAGHLTRLDYVDTFYPFV
                     VGHGSFELTAIVFSGAAGLRLGYSIINPGPFSRLDSLRLAAREVVPMIYGTVLMLVIA
                     AFLEAFWSSSTLLSSTTKYAVGAGLWALVLLYSFSGGRYEPR"
BASE COUNT          526 a          823 c          764 g          386 t
ORIGIN      
        1 gccagtcgcc gccagacgtc ggtcaattgc cagaaatact cgctcaggct gtcgatgccg
       61 cgttccgtga ccagcgccgc gcattccgtt tcggtatggc tggcgcgaaa cgcaatttcg
      121 tagcgatcga tcaggcgcga cagcgaggcg cggtagagca ggctcagcgc ctcacggtga
      181 ctgcccgcat tccacaactt catcacctgg ccgggtacgt catcgggcag gctggcgggc
      241 gtcacatcca gtccgaacag aatttccggc gcggcctgcc ggcgacgcgt gctcaccagc
      301 cgcgacaacg ggccgcggta acgccgaatc aggaaaatca gcaggccgac gaacagcagc
      361 acgagcatga gcttgagcca ggccgcggcg ccgctgaaat cggtcgacca gtcgaggttc
      421 gtttcccacc aatcgaagaa atcgatcagc cactgcggaa tttcttcgtc ttcgccggcc
      481 tcggcctggt tccagttttt gaagcgccat ttgcgcaccg tgcgctggta gccgaaatcc
      541 tcgccggtta gcacctcgtc gatcagcgcg gccgccgact cgcggtcatg ctcctgcgcg
      601 gcctcgacct gggccggcac gatggcggtt agtgtcagcg cgagcagcag cgatgccagc
      661 aggctgccgg ccccgcccgc cgcggcccgt ttgcgcgccg cgaggttgcg aaaactgatc
      721 tcgatatccc aggcttcgag ttcgatgcgg cggttcaggt agagcgcgaa gccggccatg
      781 gtatgaaacg gcaccaccag cccgatagcg gcgatgcccg ccagcgtgta catccattcg
      841 ccgaccagcg tcaggtcgcc gtagcgatcg gtgatcgcca actcgaaagc gtccggcgtg
      901 aagaatgcca gcatcaccag gatgccgaat gccaacagca cctcgaaaca gaaacagacg
      961 aactggttgc cgagcgcgac atcgctgtac ttgccgtgca gcacccgcag gcgcgcgcgc
     1021 cgctgcgcgc cgcgcaactc ctcgagtacg gtcaccgggt tgtcgaacgc gcgctgcatc
     1081 gagaagcggc gccacagcag ccacggcagc acgtccttta acggaaaact cttcatgctt
     1141 tgccagacgc cggcttcttc gtcaaacagg cggcggctgg cgatgaacag cggcaggcgc
     1201 tcccagaacg gctttagcca ccagacgatg aacagcgccc agaacggcgt ttcgcgaaac
     1261 agcagcaaca gcaccgccgc cagcggcagc atcggcagca ggccggcggc gagcaacggg
     1321 cgaaaccagg cgcgcgccat caggaaaccg agatcgaccg cctgccagcc gctgcgcgtg
     1381 caggcgttta cctgcagctt atcgaggttc atagcgaccg cccgaaaacg aatacagcaa
     1441 caccagcgcc cacaggccgg cgccgacggc atatttggtc gtgctcgaca gcagggtact
     1501 cgacgaccag aaggcttcga gaaacgcggc gatcaccagc atcagcacgg tgccataaat
     1561 catcggcacc acctcgcgcg cggcgagccg cagcgaatcc aggcgactga acggccccgg
     1621 attgatgatc gagtagccga gcctgagacc ggccgcgccg ctgaacacga tcgcggtcaa
     1681 ctcgaacgac ccgtgaccga ccacgaacgg gtaaaaggta tcgacatagt cgagccgcgt
     1741 caggtgaccg gcgatgccgc cgatgaacat gccgttgaag aacagcacca gcagcgtgcc
     1801 gatgccgacc agtatgccgc cggcgaagca gcgaaacgca acgctgatat tgttcttgat
     1861 gtaaaaaccg aacatgtaga tgtcggtatc ggcctcgcgc tcgcgcccca gcacccgcgc
     1921 ctcgggatcg tacatgcgct cgacctggcg tacctggtgc gcgtcgacca tgctgtagag
     1981 cgcttcgtcg tcgaagtagg tccacgcgcc cgccagcaga cccggcaaca ggaacaccag
     2041 cagcgcggcg aggacgaagt aacgttgacg gtagatcgcc agcggaaaat cacgccgcaa
     2101 aaacgcgaac aggttgaggc gggtttcaga gcgataacgg tagagttccc ggtaaacggc
     2161 cagcaccagg ttgttgagtc gctcgaccag cgccgcgtcg tacagccgct ccttggcgat
     2221 tgccaggtgc tggcacaaca gcatgtagtg ctcggcgagc acgcgactgt tattaccttt
     2281 cgacggctgc tgcagcgagg cttcgattct cgcccaggtc tcctcgtgct gcgcctcgaa
     2341 actgtgctgt ttcatcggac ccccaggaac caggcgccaa tccgatgcag ctgcgtgacg
     2401 ccatcctcgc cgcgcgcatg ggtgatgccc tcgaggatgc cggccagctc ctgctgccgc
     2461 ggcaccgaaa gctgctgact gcgctgcagg aaatcgata
//
LOCUS       DATCZV010000451         3242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_142724, whole genome shotgun sequence.
ACCESSION   DATCZV010000451 DATCZV010000000
VERSION     DATCZV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142724"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VIW27_10115"
     CDS             complement(<1..888)
                     /locus_tag="VIW27_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740070.1"
                     /translation="MSSPEFDIVIAGAGMVGASAAHALAGNGFRVALVEAIPPASDVA
                     SDAAYDLRVSAISPRSREILERLGVWQQLDPERVCYYEQMYIWHQHGSASVAFDAVDL
                     ARDNLGAIVENRLLQQGLYRACRQQSRIEWFMPDSVEQLLVNGTQGVELRLGSGRCLG
                     ADLLLAADGRNSPTRGLAGIEARSGKYRQSAIVANVDTELPHRHTAWQRFLSTGPLAF
                     LPLANGQSSIVWSCDDAFAAELVEADDDAFCRALEAAFEYRLGAVTGCSARLSFPLGW
                     HACERWLSGRVLLIGDAAHG"
     gene            complement(885..2105)
                     /locus_tag="VIW27_10120"
     CDS             complement(885..2105)
                     /locus_tag="VIW27_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY5740071.1"
                     /translation="MKPKTRDIAIIGGGLVGLCAALVMQYPGRRVSIIEAANLSKPQP
                     GGLNSRSIALAASSMQIFRALGISSQVEASAAPISNIHVSSRGHWGVTRLRAEDYELD
                     ALGYVIENNALSRILLEAVEASEHIELEDKASFESIAQASRVEIGYRRRDRLKRLDAD
                     LALIADGARSQARDALGIGHRIIDYDQAVVICNVEVSRPRPDSAFERFTTQGPLAMLP
                     LGGKKYACVWTLAQDRAAEICALDEPGFCAQLQDFFGHRLGLVERAGDRFSLPIQRTC
                     ADALQQLRCLLIGNAANALHPVAGQSFNLALRDIAVLYELLAEQDLAQFDDAAIEAIA
                     HQYETRRSREQRKVIRYGDGLVTLFSNELPLFDQLRAAGLGVLDILPALKAQAAYAGM
                     GMTFGGNRLLRGHR"
     gene            complement(2115..>3242)
                     /locus_tag="VIW27_10125"
     CDS             complement(2115..>3242)
                     /locus_tag="VIW27_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY5740072.1"
                     /translation="MVLIPGRKHGEYILFCREKNPEQETWNGRRAGQAGAVEIYGADD
                     SFPIDDIDDILPGLLEGTDSIYYTMGGSAEFDKRLIGWVNHIKAQSRAGLHVPSEFVS
                     LDYILHDMRLFKSREELRLMRKAAAINVRAHKRAMKLCAPGKYEYQIEAEFDHEYRSH
                     NASHAYSSIVGAGANGCILHYTENSDELRDGDLLLIDAGCEVEGYASDVTRTFPINGK
                     YSKTQRELYDLVLAAHNAAVKKVKPGNHWNDPHNAAVRVITKGLVELKLLKGSVPKLI
                     KEQAYRKFYMHRTGHWLGLDVHDVGDYKVGDQWRLLEPGMVMTIEPGIYIPAKTRGVH
                     RRWWDIGIRIEDDVLVTRQGYEILTDALPRKASEIEALMAS"
BASE COUNT          617 a         1036 c          960 g          629 t
ORIGIN      
        1 accgtgcgcg gcatcgccga tcagcaacac ccgcccgctg agccagcgct cgcaagcgtg
       61 ccagccaagc ggaaagctca ggcgcgcgct acagccggtg acggcaccga gtctgtattc
      121 gaaggccgct tccagcgccc gacagaatgc gtcgtcatcg gcctcgacga gttccgcggc
      181 gaacgcatcg tcgcaggacc atacgattga actctggccg ttggccagcg gcaggaaagc
      241 cagcgggccg gtgcttaaaa atcgctgcca ggcagtgtgc cggtgcggca gctcagtatc
      301 gacgttggca acaatcgcgg attgtcggta cttcccgcta cgagcctcga ttccggccag
      361 gccgcgcgtt ggtgagttac gtccgtcggc ggcgagcaac agatcggcgc ccaggcagcg
      421 accggagccg agccgcagct cgaccccctg cgtgccgttg accagcaatt gctccacact
      481 atcgggcatg aaccattcga tacgtgactg ttgccggcag gctcgataca ggccttgttg
      541 cagcagccgg ttttccacga ttgcacccag attatcgcgc gccagatcga ccgcatcgaa
      601 agcgacgctg gccgacccgt gctggtgcca gatatacatt tgttcgtagt aacagacgcg
      661 ctccgggtcc agctgctgcc agactccaag ccgctcgaga atctcgcgcg agcgcgggct
      721 gattgcggac acccgcaggt cataagccgc gtcgctggcg acgtcggacg ccggtggtat
      781 cgcctcgacc agcgccaccc tgaaaccgtt gcccgcgagc gcatgggcgg cgctggcgcc
      841 gaccatgccg gcgccggcaa tgacgatgtc gaactctggc gaactcaccg atggccccgc
      901 aatagccggt taccgccgaa ggtcatgccc attccggcat acgcggcctg ggcctttagc
      961 gccggcagta tatcgagcac gcccagtccg gcagcgcgta gctggtcgaa caatggcagt
     1021 tcattggaga acagcgtgac cagcccgtca ccgtagcgaa tcaccttccg ttgttcgcgc
     1081 gagcgccggg tttcgtattg atgcgcgata gcctcgatcg ccgcatcgtc gaactgcgcc
     1141 agatcctgtt ccgccagcag ttcatagagc acggcaatat cgcgcaaggc caggttgaaa
     1201 ctttgcccgg caaccggatg cagagcattt gccgcattac cgatcaacag gcacctcaac
     1261 tgttgcagtg catcggcgca ggtgcgctgg atcggtaacg agaaccggtc cccggcgcgc
     1321 tcgaccaggc ccagccggtg cccgaaaaaa tcctgcaatt gcgcgcagaa tcccggctcg
     1381 tcgagcgcgc aaatctcggc cgcacgatcc tgtgcgagag tccatacgca ggcatacttc
     1441 ttgcccccca gcggcagcat agccaaaggc ccctgggtgg tgaatcgttc gaaggcggaa
     1501 tccggcctcg gccggctgac ttccacatta cagataacca cggcctggtc atagtcgatg
     1561 atccgatgcc cgataccgag tgcgtcacgg gcctgcgagc gagccccgtc ggcgatcagc
     1621 gccaggtcgg cgtcaagccg ttttaaccga tcgcgcctgc ggtacccgat ttcgaccctg
     1681 ctcgcctgcg caatcgactc gaaactggcc ttgtcctcga gttcgatatg ctcggaagcc
     1741 tcgaccgcct ccagcaaaat ccggctaagg gcattgtttt caatcacgta accgagtgca
     1801 tcgagctcgt aatcttccgc cctgagtcga gttactcccc agtgtccgcg cgaagataca
     1861 tgtatgttgc tgattggcgc ggcgcttgct tcgacctgcg acgatatgcc aagcgccctg
     1921 aagatctgca tgctgctagc ggcgagcgca atcgaacgtg agttcaaacc gccgggctga
     1981 ggtttgctca gattcgccgc ctcgatgatg ctgacgcgac gccccgggta ttgcattacc
     2041 agggccgcgc acagcccgac caggccgccg ccgataatcg cgatatcgcg cgtctttggt
     2101 ttcatcgata aacgctaact cgccatcagg gcttcgattt cgctggcctt gcgcggcagg
     2161 gcatcggtca ggatttcgta gccctggcgc gtcacgagca cgtcgtcctc gatccgaatg
     2221 ccgatatccc accacctgcg atgcacacca cgggttttcg ccggaatgta aataccgggc
     2281 tcgatcgtca ttaccatgcc gggttcgagc aggcgccact ggtcgccaac cttgtaatcg
     2341 ccgacgtcgt gcacgtcgag tccgagccag tggccggtgc gatgcatgta gaacttgcga
     2401 taggcctgct ccttaatcaa cttcggcacg ctgcccttga gtaatttcag ctcgaccagg
     2461 cccttggtaa tgacgcgcac cgcggcattg tgcggatcgt tccagtgatt gccgggcttg
     2521 acctttttga ccgcggcgtt atgcgcggcg agcacgagat cgtagagctc ccgctgcgtc
     2581 ttcgagtact tgccgttgat cggaaaggtg cgggtaacat cggaggcgta gccctcgact
     2641 tcacaaccgg catcgatcaa cagcaggtcg ccatcgcgca gctcgtcgct gttttcggtg
     2701 taatgcagga tacagccgtt ggcgccggct ccgacgatcg acgagtaggc gtgactggcg
     2761 ttatggctgc ggtactcgtg atcgaactcg gcttcgatct ggtattcata cttgcccggc
     2821 gcacagagtt tcatcgcgcg cttgtgcgcg cgcacgttga tcgccgccgc cttgcgcatc
     2881 aggcgcagct cttcgcggct cttgaacaac cgcatgtcgt gcaggatgta atccagcgac
     2941 acgaactcgg acggcacgtg cagtccggcg cgcgactgtg ccttgatgtg gttgacccag
     3001 ccgatcaggc gcttgtcgaa ctccgcagag ccgcccatgg tgtaataaat actgtcggtg
     3061 ccctcgagca aaccgggcag gatgtcgtcg atatcgtcaa tcgggaaaga gtcgtcggcg
     3121 ccataaattt cgaccgcgcc tgcctggccg gcacgacgac cgttccaggt ttcctgttcc
     3181 gggttttttt cgcggcagaa aaggatgtac tcgccatgct tgcggcccgg aataagcacc
     3241 at
//
LOCUS       DATCZV010000452         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_672622, whole genome shotgun sequence.
ACCESSION   DATCZV010000452 DATCZV010000000
VERSION     DATCZV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672622"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VIW27_10130"
     CDS             complement(<1..2053)
                     /locus_tag="VIW27_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740073.1"
                     /translation="MSRFPDLGGSNLQLRLVGKNYLEFAALLGLGEVAALRPEPFELR
                     SSLDYTAQGRQLFTARLALADLNGEFDGELTDDPAYAGSHLDYRLEGRNDGLLQRILG
                     RPTGVEGSYNLQGKLRRSPLGYDIQQAALTFGANALEINGTLGAEPLRADSRLTAHFR
                     GPDLDKIAAIAGYDGFVPAGIVEIDASAQVRDDAIQVDGLVARLGRNRLQASGLVRLQ
                     DAAVGSRVEVALSGEDIVDVLPPDLLAFVDPQQAFELSGALAVGSGSLEVDGLQARLG
                     EVVLEGSGRVSTTQPMTAASLRFNARGPDLAAIVPENLLPYRLPAAEFAVTGGVALAP
                     GGLLLDGIEARIGADRVGVSGTIPLETPTEGLDLAVTASGPNLRNTVPAELNQFDFAE
                     QPFDIAGEIGLAKGILSLRQVEVSAPRGRLSGQVNVAIDNPRRFGEFDLKASGSDLAE
                     FSASMLEYRPANVAFDLDARGSWNSERIKIERGLILLGDANIEVRGEVDLPPGVTATR
                     LVIAARGSNLADLGQYGELALPEDEFDIEARLSGNADQLQIPRLDISIGDSDLRGSLY
                     LTFAEKPKIQIELESEKLELVKLLPPNDGEGENEPPQPLPSDGRLIPRLAVPVDQLQR
                     IDLEARVRMGEMQLRNSILRDIEIDTRLQNGELTVSRLQARAREGRLVAQFRAAAVGE
                     RI"
BASE COUNT          393 a          772 c          664 g          427 t
ORIGIN      
        1 ctatccgctc gcccaccgcg gctgcccgaa attgggcaac gagccgcccc tcgcgtgccc
       61 gggcctgcaa gcggctgacc gtcaattcgc cgttctgcag acgggtatcg atttcgatat
      121 cccgcaggat actgttgcgc agctgcatct cgcccatcct gaccctggcc tccaggtcga
      181 tccgttgcag ctgatcgacg ggaaccgcca gtcgcggtat cagccggcca tcgctcggca
      241 gcggttgcgg cggctcgttt tcaccctcgc cgtcattcgg cggcaacagt tttaccagct
      301 ccagtttttc cgattccagc tcgatctgga ttttcggctt ttcggcaaac gtcaggtaaa
      361 gcgaaccgcg caagtcactg tcgccgatgc tgatatccag ccgcggaatt tgtagctgat
      421 cggcgttacc gctcaacctg gcttcgatat cgaattcgtc ttccggcagt gccagttccc
      481 cgtactggcc gaggtcggcc aggttactgc cgcgcgccgc gatcaccagc cgggtcgcgg
      541 tcacgccggg cggcagatcg acttcgccgc gcacctcgat gttggcgtcg ccgagcagaa
      601 ttaaccctcg ttcgatcttt atcctttcac tattccagct gccgcgcgca tcgagatcga
      661 aagccacgtt cgccggccga tattcgagca tcgacgccga aaactcggcc agatcgctgc
      721 cgctcgcctt gaggtcgaat tctccgaagc ggcgcggatt gtcgatggcc acgttcactt
      781 gtccgctcag cctgccccgc ggagccgata cttcgacctg ccgcaaagac agtatccctt
      841 tcgcgagacc aatctcgccg gctatatcga acggctgttc ggcgaaatcg aactggttca
      901 attccgccgg cacggtgtta cgcagattcg ggccgctcgc ggttaccgcg aggtccaggc
      961 cttcggtcgg ggtttctaac ggtatggttc cggacacgcc cacgcgatcg gcgccaatcc
     1021 gcgcctcgat cccatcgagt agcagcccgc cgggggccag tgccacgccg ccggtcacgg
     1081 caaactctgc cgccggcagg cgatacggca gcaggttttc gggaacgatt gccgccaggt
     1141 ccgggccgcg ggcattaaag cgcagcgacg cggcagtcat cggctgcgtc gtcgataccc
     1201 tgcccgagcc ttccagaacc acctcgccga gcctggcctg caggccgtcg acctcgagac
     1261 tgccgctgcc caccgccagg gctccgctca gttcaaaggc ttgttgcggg tcgacgaacg
     1321 cgagcagatc gggcggcagc acatcgacga tatcttcgcc gctcaacgcg acctcgacgc
     1381 ggctgccgac cgcggcgtcc tgcaacctga ccagcccgga tgcctgcagc cggttgcggc
     1441 cgagccgcgc cacgagtcca tcgacctgaa tcgcatcgtc acgaacctgc gccgacgcgt
     1501 cgatttcgac tattcccgcg ggcacgaatc cgtcgtatcc ggcgatggcg gcaattttat
     1561 ccaggtccgg accgcgaaaa tgcgctgtca ggcgggaatc ggcgcgcagc ggttctgctc
     1621 ccagcgtgcc attgatttcg agcgcgttgg caccgaaagt gagcgcggct tgttgaatgt
     1681 catatcccaa aggactgcgc cgcagcttgc cctgcaggtt ataactgccc tcgacacccg
     1741 tgggccggcc gagtattcgc tgcaacaagc catcgttgcg gccctcgagc cggtagtcga
     1801 gatgcgaacc cgcatacgcc ggatcgtcgg tcaactcgcc atcgaactcg ccgttgagat
     1861 cggcaagtgc cagccgggcg gtaaatagtt gcctgccctg cgcggtgtag tccaggctgg
     1921 aacgcaattc gaacggctcg ggtctcaacg ccgccacttc gccgaggccg agcagcgcgg
     1981 cgaattccag gtaattcttg ccgaccagtc gcaattgcag attgctaccg ccgagatccg
     2041 gaaatcggct catcgtcccc tcggcggtca aatgattgtc tccgctgtcg aagcgtgtct
     2101 tgccgagcgt taattgctgg cccgacagcg acgccctcga ttcgaattca aacggctgcc
     2161 cgggcaagcc cggcaatccc gccagccgtg ccgccgcccc gaggtccggg cccgccgcgg
     2221 aaagctcgat cgatgccccg tcgatggaat ccaggg
//
LOCUS       DATCZV010000453         4187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_725704, whole genome shotgun sequence.
ACCESSION   DATCZV010000453 DATCZV010000000
VERSION     DATCZV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725704"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VIW27_10135"
     CDS             complement(<1..709)
                     /locus_tag="VIW27_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5740074.1"
                     /translation="MTLEKLLDGLMVDVEPFVICRSAAGPKLKLPALQFASLHYVVAG
                     RGTLLIDARAPIALDTGSVVILPAGLAHELVGDGDEDAALAAARRCQPAALGMDEISS
                     DGDGGIVLACSAINATYQRTRGLFDYLDEPIVSPMRAGDPLAGTLDSMLAEMADPGPG
                     SASLVALLMKQCLIHVLRTYCDSGHCRVPWLGALEDARLALALECMLDEPGRRYTLEL
                     LAATAGMSRSAFAQHFRD"
     gene            848..1696
                     /locus_tag="VIW27_10140"
     CDS             848..1696
                     /locus_tag="VIW27_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY5740075.1"
                     /translation="MKQSQSEQQHLDNEQPQVPARRRALKAGLAGAGIAAAGLGGIAG
                     SARAAETNPYADPEKPALPPSNMQLDLQRTALVVTDPQIDFLSPDGVTWGVVGESVTE
                     HGTVDNIGRLFAAAKNAGMVVAVSPHYYYPADKGWKFEGALEKLMHAIGMFDRKSPYS
                     GEGFENSGADFMPEYKPFIHDGKTIIASPHKVYGPEQNDLVLQLRKQRVDQVILAGMS
                     ANLCVQAHLHELLETGFEVAVVKDATAAAKLPEGDGYLAALTNFRYMANAVWTTDEAV
                     ARIAAS"
     gene            1758..2039
                     /gene="grxC"
                     /locus_tag="VIW27_10145"
     CDS             1758..2039
                     /gene="grxC"
                     /locus_tag="VIW27_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY5740076.1"
                     /translation="MQHVEIYTKSWCPYSVRAKALLTARNLEFTDIDVTADAEREAEM
                     IARAGRSSVPQVFIDGEHVGGYDDLAGLDARAGLDRIVAAGVEAAVSGG"
     gene            complement(2086..2631)
                     /locus_tag="VIW27_10150"
     CDS             complement(2086..2631)
                     /locus_tag="VIW27_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY5740077.1"
                     /translation="MMQRGSIAAVSLFANCLLIGALACTAGQATASSEDSAAEAGKKA
                     RLIDLWKPGDSGQRMNIRGRVTSLDGTPLAGVKVEIRQPDGDGLWTEQYRTTLTTDAK
                     GRYQFGSVVPVSNYCGEPHVEVVVYQRGWEYFDDKLVFEEVLTDDSFYFGDGTPVFLE
                     ESSVNGETIKFGRFDIMLSPE"
     gene            2793..3485
                     /locus_tag="VIW27_10155"
     CDS             2793..3485
                     /locus_tag="VIW27_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740078.1"
                     /translation="MIGSQHFSRRIVLLAASASAAGLGAHAQQPEMTFFITSAGPGDG
                     ANLGGLEGADAHCGKLAAAAGSKLTNWRAYLSVAASIDRSSGKARVIPGVNARDRIGN
                     GPWHNASGQLIARSVDELHSAANRISKSTGLDEHGKPVNDRSMRPNYHDILTGSDPQG
                     MYSTAGADTSCSGWTSNGQGSAIVGHHDRAGLSLDWHMLSWNSAHGTVGCGRDDLPKT
                     GGNGLFYCFNAD"
     gene            complement(3699..>4187)
                     /locus_tag="VIW27_10160"
     CDS             complement(3699..>4187)
                     /locus_tag="VIW27_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY5740079.1"
                     /translation="TTAAGKAVGIPDIEDLENNIHAGVKYLHWLRETYFSDESIEPLD
                     RVLFSFAAYNAGPGNMRKARRRAKRLGFDPDRWFGNVEIGMFRAVSGEPASYVRNIYK
                     YYVTYQGLEQTRQSREKALENHAQESAASGQPWIAAVLVLALLGLLGVAWQRFSKPSR
                     SA"
BASE COUNT          757 a         1355 c         1365 g          710 t
ORIGIN      
        1 cgtcgcgaaa gtgttgtgcg aaggccgagc ggctcatgcc ggcggttgcc gcgagcagtt
       61 ccagcgtgta gcggcgtccg ggttcgtcga gcatgcattc gagcgcgagc gccagccgcg
      121 catcctccag cgcgccgagc cagggcaccc ggcagtggcc gctgtcgcag taagtgcgca
      181 gtacgtggat caggcactgt ttcatcagca gcgcgaccag tgacgcgctg ccggggccgg
      241 gatcggccat ctcggcgagc atcgagtcga gggtgcctgc cagcggatca ccggcgcgca
      301 tcgggctgac gatcggttcg tcgaggtaat cgaacagacc gcgggtgcgc tgataagtgg
      361 cgttgatcgc gctgcaggcg aggacgatgc cgccatcgcc gtcgctcgag atttcgtcca
      421 tgccgagcgc ggccggctgg cagcggcgcg cggcagcgag cgccgcatcc tcgtcgccgt
      481 caccgacgag ctcgtgggcg agacccgccg gcaggatgac gacgctgccg gtatccagcg
      541 cgatcggcgc acgagcgtcg ataagcagcg tgccgcggcc agcgacgacg tagtgcaggc
      601 tcgcgaactg caatgccggc agcttcagtt tcggtcctgc cgcgctgcgg cagatcacga
      661 acggctctac gtcaaccatc aggccgtcga gtaatttctc gagcgtcatc gtcgccaagc
      721 gcttctcccg tgccgcagtt cgccgtcgtg cattgcgcgc tggacgatat cgaacacatc
      781 gcgtcccatt gcgcacaccg ccgtctttac catgggctca ccttgtacgc aacatcagga
      841 gataaccatg aaacaatcgc aatccgaaca acaacacctc gataacgagc agccgcaggt
      901 cccggcccgg cggcgcgcgc tgaaagccgg cctcgccggt gccggcatcg ccgcggcggg
      961 gctcggcggc atcgccggca gcgcgcgcgc agcggagacc aatccctatg ccgacccgga
     1021 aaagccggcg ctgccgccgt cgaacatgca actcgacctg cagcgcaccg cgctggtggt
     1081 caccgatccg cagatcgatt tcctcagccc cgacggcgtg acctggggcg tggtcggcga
     1141 aagcgtcacc gagcacggca cggtcgacaa catcggccgc ctgttcgcgg ccgccaagaa
     1201 tgccggcatg gtggtcgcgg tatcgccgca ttactactac ccggcggaca agggctggaa
     1261 gttcgaaggc gcgctcgaaa agctgatgca cgcgatcggc atgttcgacc gcaagagccc
     1321 ttattccggc gaaggcttcg aaaattcggg tgccgatttc atgcccgagt acaaaccctt
     1381 cattcacgac ggcaagacga ttatcgcctc accgcacaag gtctacgggc ccgagcagaa
     1441 cgatctcgtg ctgcagctgc gcaagcagcg cgtcgaccag gtcatcctcg ccggcatgtc
     1501 ggccaatctg tgtgtgcagg cgcacctgca cgaactgctc gagaccggct tcgaggtggc
     1561 ggtcgtcaag gatgccaccg cggcggccaa actgccggaa ggcgacggct acctcgccgc
     1621 gctgaccaac ttccgctaca tggcaaacgc ggtctggacc accgacgagg ccgtggcgcg
     1681 aatcgccgca agttgacacc tgtcggcatt ccccggcgag attcgccggg gaatgccgct
     1741 atacccggag aaacccaatg caacacgtag aaatctacac caagagctgg tgcccctact
     1801 cggtcagggc gaaagccctg ttgacagcgc ggaatctcga gttcaccgac atcgacgtga
     1861 ccgccgatgc cgagcgcgaa gccgagatga tcgcgcgcgc cgggcgcagc agcgtgccgc
     1921 aggtgttcat cgatggcgag cacgtcggcg gctacgatga cctggcaggc ctcgacgcgc
     1981 gcgccgggct ggatcgtatc gtcgcggccg gcgtcgaagc ggctgtcagc ggcgggtgag
     2041 agcgcgttgt tcgaagatcg ccgccgggcc tttgcaccag gcgggttact cgggcgacag
     2101 catgatgtcg aagcgcccga acttgatggt ttcgccgttg acgctggatt cctcgagaaa
     2161 caccggcgtg ccgtcaccga agtagaaact gtcgtcggtc aacacttctt cgaacaccag
     2221 cttgtcgtcg aagtattccc agccacgctg gtagacgacg acctcgacgt gtggttcacc
     2281 gcagtagttg ctgaccggca ccaccgagcc gaactggtag cgccccttgg cgtcggtggt
     2341 cagcgtggtg cggtattgct cggtccacag accatcgccg tcgggctggc gaatctcgac
     2401 cttgacgccg gccagcgggg tgccgtcgag actggtaaca cggccgcgaa tattcatgcg
     2461 ttggccgctg tcgccgggtt tccacaggtc gatcagcctg gcttttttgc cggcctcggc
     2521 ggccgagtcc tcggaactgg ccgtcgcctg gccggcggtg cacgccagcg cgccgatcag
     2581 caggcagttg gcgaataggg atacagcggc tatcgatcct cgttgcatca ttatcggctc
     2641 ctcgcagatt gacggaaaat cgattataac tgccaaagcc gtaaatgatg aaatccccga
     2701 gtccggccct gtccggtgcc cgggcccgga accaggcggt ttcggggcga tcgatgcatt
     2761 tcggctgaca tcaactactc gaggacaagt cgatgattgg ttcgcagcat ttttcccgtc
     2821 gtatcgtttt actggccgca tccgcatccg cagccggtct cggtgcccat gcgcagcagc
     2881 cggagatgac cttcttcatc accagcgccg gtcccggtga cggcgccaat ctcggcggtc
     2941 tcgaaggggc cgacgcccat tgcggcaagc tcgccgccgc ggccggctcg aagctgacca
     3001 actggagggc ttatctgagc gttgccgcga gcatcgaccg atcgagcggt aaggccaggg
     3061 tgattccggg cgtcaatgcg cgtgaccgca tcggcaacgg tccctggcac aatgccagcg
     3121 ggcagctgat cgcccgcagt gtcgacgagc tgcattccgc cgccaaccgg atcagcaaga
     3181 gcaccggcct cgacgagcac ggcaagcccg tcaacgatcg cagcatgaga cccaactatc
     3241 acgatatcct caccggctcg gatccgcagg gcatgtattc gaccgccggc gccgatacca
     3301 gctgctccgg ctggaccagc aacggccagg gctcggccat cgtcggtcat cacgaccgtg
     3361 cgggcctgag cctcgactgg cacatgctgt cgtggaattc ggcgcatggc accgtcggct
     3421 gcggtcggga cgatctgccg aagaccggcg gtaacggcct gttttattgc ttcaacgcgg
     3481 attaaaaccg tctgcagctc ctcgccgcag cgcccaggcg cgcggcgagg agcttatttt
     3541 ggtgcccgag gcaggcctcg aaactgcagt cgaagctgcg cactcgtgga ctgaggctgg
     3601 cggcaagacc cgtgtgcggc ggtcgattcg cgcagcaccg gccggccgtt atgccgggcg
     3661 cggatcagcc gcgcggtgtc ggcggggcgt cgcggctttc aggccgatcg cgaaggtttc
     3721 gagaatcgct gccaggccac accgagcagg ccgagcaggg cgagcaccag cacagccgcg
     3781 atccacggct ggccgctggc cgccgattcc tgggcgtgat tttcgagcgc tttttcgcgc
     3841 gactggcggg tttgctcgag gccctggtag gtgacgtaat acttgtagat gttgcgcacg
     3901 taggacgccg gctcgccgct gactgcgcga aacatgccga tctcgacgtt gccgaaccag
     3961 cgatcgggat cgaagccgag ccgtttggcg cgccgccgcg ccttgcgcat gttgccgggg
     4021 ccggcattgt aggcggcgaa cgaaaacagc actcggtcga gcggctcgat ggactcgtcg
     4081 gagaaataag tctcgcgcag ccagtgcagg tatttgaccc cggcgtgaat attgttctcg
     4141 aggtcctcga tatcgggaat gccgaccgct ttgccggccg cggtggt
//
LOCUS       DATCZV010000454         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_38330, whole genome shotgun sequence.
ACCESSION   DATCZV010000454 DATCZV010000000
VERSION     DATCZV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38330"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VIW27_10165"
     CDS             complement(<1..1556)
                     /locus_tag="VIW27_10165"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY5740080.1"
                     /translation="MNDIQPLWTPSAERIATANLTRFIEQVNRNHGLALADYASLYRW
                     SIDDKEKFWSEIWDFCGIVGDKGERILVDGEEIERAQWFVDAHLNVAENLLRRRDDSI
                     ACTFRAEDQVEYALTWEQLHQQVASVAAWLKQQGLQPGDRVAAYLPNMPEAVVAMLAA
                     TSLGAVWTSTSPDFGAESVIDRFGQTEPRFLFVADGYFYNGKRHELASRIREIRAQLP
                     SVEQLVMIELAGFGNQVDAVDWRDIVAEPGGEIEFVPRAFNDPLYVLYSSGTTGKPKC
                     ITHRVGGTLIQHLKEQMLHCDIHPGERVFFFTTLGWMMWNWLVSALASEATLMLYDGS
                     PFYPDGEVLWRYADDISLSFFGTSAKYLDALKNAGIKPMDKFELSALRALGSTGSVLA
                     PESFDYVYDNIKRDLCLASMSGGTDIVSCFVLSCPLLPVYRGEIQCRGLGMAVDVFDD
                     DGNPVRNQKGELVCTQTFPCQPAFFWGDDGGEKYHNAYFARFDNVWHHGDYVMLTERD
                     GIVIYGRSDAT"
     gene            complement(1626..1760)
                     /locus_tag="VIW27_10170"
     CDS             complement(1626..1760)
                     /locus_tag="VIW27_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740081.1"
                     /translation="MMAQVVIRAGEPVEVVERVANDRQDIGSGARRRTGYQFTLLEFE
                     "
     gene            complement(1952..2299)
                     /locus_tag="VIW27_10175"
     CDS             complement(1952..2299)
                     /locus_tag="VIW27_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740082.1"
                     /translation="MNISHNVKPAIWGMVAGAVATMVVGFSWGGWITRGTAGELKTAS
                     ANAAVVEVLTPRCVAHAEQQTDKLAVLRQESSWKYRDFVVSAGWVDDVVDNYQYEVAI
                     ACANKLVGDDATG"
BASE COUNT          490 a          780 c          800 g          493 t
ORIGIN      
        1 gtcgcgtcgg agcggccgta gatgacgatg ccgtcgcgct cggtgagcat cacgtagtcg
       61 ccgtgatgcc agacgttgtc gaaacgcgcg aagtaggcgt tgtggtattt ctcgccgccg
      121 tcgtcgcccc agaagaacgc cggctggcac ggaaaggttt gcgtgcagac cagctcgccc
      181 ttttgattac gcaccgggtt gccgtcgtcg tcgaacacgt cgaccgccat accgagcccg
      241 cggcactgga tttcgccgcg gtaaaccggc agcagcgggc aggagagcac gaagcacgag
      301 acgatgtcgg taccgcccga catcgacgcc aggcacaggt cccgcttgat gttgtcgtag
      361 acgtagtcga aactctccgg tgccagcacc gagcccgtcg aacccagcgc gcgcagcgcc
      421 gacagctcga acttgtccat cggtttgatg ccggcgtttt tcagcgcatc gaggtacttg
      481 gccgaggtgc cgaaaaagct taaggagata tcgtcggcgt agcgccacag cacttcgccg
      541 tcggggtaaa aaggcgaacc gtcgtagagc atcagcgtgg cctcgctcgc cagcgcgctg
      601 accagccagt tccacatcat ccagccgagc gtggtgaaaa agaacacgcg ctcgccgggg
      661 tgaatatcgc aatgcagcat ctgttccttg aggtgctgta tcagggtgcc gccgaccctg
      721 tgagtgatgc acttcggctt gccggtggtg ccggacgagt agagcacgta gagcggatcg
      781 ttgaacgcgc gcggcacgaa ttcgatctcg ccgccgggct cggcgacgat gtcccgccag
      841 tcgaccgcgt ccacctggtt accgaagccg gcgagctcga tcatcaccag ctgctcgacg
      901 ctcggcagct gtgcgcgaat ctcgcgaatc ctggaggcca gctcgtggcg cttgccgttg
      961 tagaagtaac cgtcggcgac aaacaggaag cgcggctcgg tctggccgaa gcggtcgatc
     1021 acgctttcgg cgccgaaatc gggcgaggtc gaggtccata ccgcgccgag gctggtggcg
     1081 gcgagcatcg ccaccacggc ctcgggcatg ttgggcaggt aggcggcaac gcggtcgccc
     1141 ggctgcagtc cctgttgctt cagccaggcg gcgacgctgg cgacctgctg atgcaattgt
     1201 tcccaggtga gcgcgtactc gacctggtcc tcggcgcgaa acgtgcaggc gatggaatcg
     1261 tcgcggcggc gcagcaggtt ctcggcgacg ttgaggtgcg cgtccacaaa ccattgcgcg
     1321 cgctcgatct cctcgccgtc gacaagaatg cgctcgccct tgtcgccgac gatgccgcag
     1381 aaatcccaga tctcggacca gaatttttcc ttgtcgtcga tcgaccagcg gtaaagcgag
     1441 gcatagtcgg ccagtgcgag gccgtgattg cggtttacct gctcgatgaa gcgcgtcagg
     1501 ttggcggtgg cgatgcgctc cgcgctcggt gtccacaggg gctggatatc gttcatgctg
     1561 tcatccgcga atcgctgggt tcggtaatat taccaaaccc aagattaggg ggacacttta
     1621 gccgatcact cgaattcgag cagggtaaac tgataacctg tccgcctgcg ggcgcccgag
     1681 ccgatatctt gccggtcgtt ggcgacccgc tcgacaacct cgaccggttc gccggcccgg
     1741 atcacaacct gcgccatcat caggcacggc aggatctacg ggagcaccgc cagccgggcg
     1801 agcacggtaa ccggtggttg acgaaaccac tcgcaaagca gcgaatcacg ttgacggaat
     1861 ggcaggggtg ctctgcgaac ccgggcggag aaatccctga tttccggtcg gcgtacaggc
     1921 cggttcggaa atcggggtgt cgcggaattc gctagccggt tgcgtcgtcc ccgaccagct
     1981 tgttggcgca ggcgatggcg acttcgtact ggtagttgtc gaccacgtca tcgacccagc
     2041 cggccgagac gacgaagtca cggtacttcc agctgctctc ttgcctgagc accgccagct
     2101 tgtcggtttg ctgttccgcg tgggccacgc acctgggcgt aagcacttcg accactgccg
     2161 cgtttgcgct cgcggtcttt agctctcctg cagtgccccg cgttatccag cccccccagg
     2221 aaaagccgac taccatcgtg gctactgcgc cagcgaccat gccccagatt gcgggtttta
     2281 cattgtgtga aatattcatt ggcgatcctc tcgtcatttg ttggttgcga taaccgcgga
     2341 gaggtggcct gcttcgaaca gcggccatgc gcaccggcgg ttaatgccgt gcaataccgc
     2401 caatcgggac cttattcgga agaaactgtc gaacagaaag ataggggcaa gtaagctacc
     2461 ttatacctat tttcgcgaga tagcgaatct aaaacgccgt aaacagaatc ctgctcgttg
     2521 ccgtcgccat gaacggcgat gattaattcg ttaagcgttt cgc
//
LOCUS       DATCZV010000455         6099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_779643, whole genome shotgun sequence.
ACCESSION   DATCZV010000455 DATCZV010000000
VERSION     DATCZV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779643"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VIW27_10180"
     CDS             complement(<1..641)
                     /locus_tag="VIW27_10180"
                     /inference="COORDINATES: protein motif:HMM:NF037360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740083.1"
                     /translation="MTTRDSLLVPDRNTCMVSITDARDRILRRIARSLVRDEPALAVL
                     SPYAPGMHGDMLDTPSLVIEDHGSIRLFEDSGNRAYSYRALLLAGDDDLVAIGAARER
                     EFEAYCRDYLGLGNPRVLVAAPAAGDASLASRCREDAAFTAEAAAHAEAHGGLNILPY
                     MGSWPVWELAGTIARRCRAPLRVIAPPPQLTRRVNDKIWFTRLLRSVFGPTSIP"
     gene            complement(953..2257)
                     /locus_tag="VIW27_10185"
     CDS             complement(953..2257)
                     /locus_tag="VIW27_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5740084.1"
                     /translation="MEIANLVIVLSAVLFAALLAGVWVAATLFLVAFVALSLNGNDNI
                     GLLMATTTWGASATWTLAALPLFIWMGEILFRTRLSEDLFHGLAPWLKRVPGRLLHVN
                     ILGCTIFAAVSGSSAATAATIGRMTFPELQRRGYPQRMTIGTLAGSGTLGFLIPPSII
                     LIVYGVSAEVSIARLFIAGVLPGAMLVVLFMGYTATWSILNSSQLPADDSPDLPLKQK
                     LYLTRRLFPVLGLILFVLGSIYAGWATATEAAALGVFGALFLSWVTGNLSRETFMASL
                     LGATRTSCMITFIIAGAAFLSLAMGFTGLPRLLAEEIAALGLSPVMLIVALTVFFMLL
                     GCFLDGISIVVLTTSIVLPMIERSGIDLLWFGIYVVLVVEMSQITPPVGFNLFVIQGL
                     TKVDIIRIARYALPFFLLLMTAIVLLTAFPGIATWLPARMTA"
     gene            complement(2263..2760)
                     /locus_tag="VIW27_10190"
     CDS             complement(2263..2760)
                     /locus_tag="VIW27_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY5740085.1"
                     /translation="MRSFLDRLYFGAGAVAGACIVLITVMILAQIIGRWFGVIIPSTE
                     DFSGYLLAAATFLALAYTFRNGGHIRVTLLIHRLSSPLQRRLTYAVLLLFAVIMGWGA
                     WHVCYLVYESWSFEELSQGYIAIPLWIPQVPMALGAVLFFVALADDLICSLRGATPTF
                     LELEE"
     gene            complement(2781..3752)
                     /locus_tag="VIW27_10195"
     CDS             complement(2781..3752)
                     /locus_tag="VIW27_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5740086.1"
                     /translation="MTGKHLFTAAALGAVLCATTAFAETKWDMPTPYGDGVHHTQNVR
                     QFADDLRAATGGELNIVVHSGASLIKHPEIHRAVRTGQVPIGEMFMGLLGNDNPVFKA
                     DNIPFMASDFASARQLWDATRPHIEQALQKDGLKLLYAVPWPPQGFYTKQPISSAADL
                     EGLKMRAYSPTTSRLAVLLKATPTTVQTPEIPQAFSTGIIDAMVTSPSTGVSSQSWDY
                     VSNYTDTQAWIPKNMVIVNNRAFKRLDKDLQQAVMQAAAAAEARGWEMAQAETAAKTA
                     TLAENGIAVAEPSAQLKADLQAIGKTMSEEWAAEAGEVGQQILKAYR"
     gene            complement(4024..5502)
                     /locus_tag="VIW27_10200"
     CDS             complement(4024..5502)
                     /locus_tag="VIW27_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008384989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HEY5740087.1"
                     /translation="MTIKLETEIVNQEVYDRAVERFRQAAIKLPTISQLADPLSSRAA
                     IESQIQSADPDSPDARNLFRVHWHNAADRKNLVDVPGHIVLPPELTGVDAKIVVAIGN
                     RFPMIRAHKVLAAYGCLVPRLISGQFDPTRHRAVWPSTGNYCRGGVAISRILGCRSVA
                     VLPEGMSRERFEWLENWVTDQSDIVRTYGTESNVKEIYDACNLMATDTENVILNQFNE
                     FGNYISHRAVTGPALERIFDAVNSNGKMQARAYVAASGSSGTLAAGDYLKAQLGTDIC
                     VVEALECPTLLNNGYGDHNIQGIGDKHVPFIHNVLNTDYVVAVTDKASNALNLVFNTA
                     VGRDFLLKRTGISGELIDGLADLGLSSIANVLGAIKMSRYLGLGADDVVLTVATDGAE
                     MYRTEIARTTEQDFGGSFDELAAAESYGRYLLATTTDHMEELSLRGRERIFNLGYYTW
                     VEQQGVSLQDFDARRDPEFWNRLMEVVPAWDRMIDEFNSAAS"
BASE COUNT         1231 a         1845 c         1928 g         1095 t
ORIGIN      
        1 ggtattgacg tcggcccgaa cacggatcgc agcagtcgcg taaaccagat cttgtcgttg
       61 acacggcgag tcagctgcgg aggcggagcg atcacgcgaa gcggtgcgcg acagcgtcgc
      121 gcgatcgtgc cggcaagctc ccagaccggc caggagccca tgtaaggcag aatgttgagc
      181 ccgccgtgag cctcggcgtg ggctgccgcc tcggcggtga atgccgcgtc ttcgcggcaa
      241 cgggaggcca gcgaagcgtc gccggcggcc ggtgcggcaa ccagcacgcg cggattgccc
      301 agcccgagat agtcgcggca gtaagcctcg aattcgcgct cgcgcgccgc gccgatcgcg
      361 accaggtcgt cgtcgccggc caacaacagc gcgcggtagg agtaggcccg attgccgctg
      421 tcctcgaaca gtcgaatact gccatgatcc tcgataacca ggctcggcgt atcgagcata
      481 tcgccgtgca tgccgggcgc ataggggctc agcaccgcca gcgccggttc gtcccgcacc
      541 agcgagcggg cgattcgccg cagtatccgg tctctcgcgt cggtgatcga aaccatgcag
      601 gtatttcgat cgggcaccag cagagagtca cgggtagtca cggcgcaggc attgccagaa
      661 cctgattgcc gtagcaaacc agcatcgatg ccccgtcacc gataaaagcg agacgagcaa
      721 atctcaaagg cggcctgcta gccgctgccg gggaagcaac tgatgcggcg gtttcagcag
      781 aggtcatgat cgacatcgaa attcgagtca gccgatcata ctcaaggact attgggctgt
      841 ctaattgcgc cgggaccgcg agatcaggcc ccgcatctga ggaacatcaa aacggcaatg
      901 ccctatccag ggccaggatc cgctgttcag gccgggtatg acgtcgttcg ggtcaggctg
      961 tcatgcgggc cggcagccag gtcgcgatgc cgggaaaggc agtcagtagc acgatggcgg
     1021 tcatcaacag caggaaaaag ggcagcgcgt agcgcgcgat gcggatgatg tcgaccttgg
     1081 tcaggccctg aatcacgaac aggttgaagc cgaccggcgg cgtgatctgc gacatctcga
     1141 ccaccagcac cacgtaaatg ccgaaccaca gcaggtcgat gccgctgcgc tcgatcatcg
     1201 gcagcacgat agacgtggtc agcacgacga tagagatgcc gtcgaggaaa cagccgagca
     1261 gcatgaagaa cacggtcagc gcgacgatca gcatcaccgg cgacaggccc agcgcggcga
     1321 tttcctcggc cagcaggcga ggcaagccgg tgaaacccat cgccaggctc aggaaagccg
     1381 cgccggcgat gatgaaggta atcatgcacg aggtgcgggt cgcaccgagc aggctggcca
     1441 tgaaggtctc gcggctcagg ttgccggtca cccaggacag gaacagcgcg ccgaacacgc
     1501 cgagcgcggc ggcctcggtg gcggtcgccc agccggcgta aatcgaaccc agcacgaaca
     1561 ggatcaggcc gagcaccggg aacagccgtc gcgtcaggta gagcttttgc ttaagcggca
     1621 ggtcggggct gtcgtccgcc ggcagctggc tcgagttcag gatcgaccag gtcgccgtat
     1681 agcccatgaa tagtaccacc agcatggcgc cgggcagcac cccggcgatg aacaggcgcg
     1741 cgatcgatac ctcggcggag acgccgtaga cgatgaggat gatcgacggc gggatcagga
     1801 agccgagcgt gccggagccc gccagcgtgc cgatcgtcat gcgctgcggg tagccgcgcc
     1861 gctgcagctc ggggaaggtc atgcggccga tggtcgccgc ggtcgctgcc gacgagcccg
     1921 acacggcggc gaagatcgtg cagccgagga tgttgacgtg cagcagccgg ccgggcacgc
     1981 gcttcagcca cggcgccaga ccgtggaaca ggtcctcgga gagccgcgtg cgaaacagga
     2041 tctcgcccat ccagatgaac agcggcagcg cggccagcgt ccaggtcgca gaagctcccc
     2101 aggtggtggt cgccatcagc agcccgatgt tgtcgttacc attcaggctg agcgcgacaa
     2161 aggccaccag gaacagggtc gcggcgaccc agacacccgc cagcagcgcg gcgaacagga
     2221 ccgcggacag cacgatgacg agattggcga tttccaccgc cgctattcct cgagctcgag
     2281 aaaggtcggc gtcgcgcctc tcagcgagca gatcaggtcg tcggccaggg cgacgaaaaa
     2341 cagcaccgcg ccgagcgcca tcggcacctg tggtatccac agcggtatcg cgatatagcc
     2401 ctgcgacagt tcctcgaaac tccaggattc atagaccagg taacagacgt gccacgcgcc
     2461 ccaacccatg atcaccgcaa acagcagcag caccgcgtag gtcagccggc gctgcagcgg
     2521 tgacgacagg cggtggatca gcagcgtgac gcgaatatgg ccgccgttgc gaaaggtata
     2581 ggcgagcgcc aggaaggtgg ccgcggccag caggtaaccg gaaaaatcct cggtcgacgg
     2641 aatgatgacg ccgaaccagc ggccgatgat ctgcgccagg atcatcaccg taatcagcac
     2701 gatacaggcg ccggcgacgg cgccggcacc gaagtagaga cggtcgagaa agctgcgcac
     2761 ggttggcgcc cgcgccggac ctagcggtag gctttaagga tctgctgccc cacctcgccg
     2821 gcttccgcgg cccattcctc gctcatcgtc ttgccgatcg cctgcaggtc cgcctttagc
     2881 tgggccgagg gttcggccac cgcgatgccg ttttcggcca gcgtcgcggt cttcgccgcg
     2941 gtctccgcct gcgccatttc ccagccgcgc gcctcggcgg cggcagcggc ctgcatcacg
     3001 gcttgctgca gatccttgtc caggcgtttg aaggcgcggt tgttgacgat caccatgttc
     3061 ttcggaatcc aggcctgggt atcggtgtag ttggagacat agtcccacga ctggctgctg
     3121 acgccggtgc tcggcgaggt taccatcgcg tcgatgatgc cggtcgaaaa agcctgcgga
     3181 atttcggggg tctgcacggt ggtcggggtc gccttcagca gcaccgcaag ccgcgaggta
     3241 gtcgggctgt aggcacgcat tttcaggccc tcgaggtcgg ctgccgagct gatcggttgc
     3301 ttggtataga agccctgcgg gggccagggc accgcgtaga gcagcttgag gccgtccttt
     3361 tgcagtgctt gctcgatgtg cgggcgggtc gcgtcccaga gctgcctggc gctggcgaag
     3421 tcggaggcca tgaacggaat gttatccgcc ttgaataccg ggttgtcatt acccagcagg
     3481 cccatgaaca tttcgccgat cggcacctgg ccggtgcgca ccgcgcggtg aatctcgggg
     3541 tgcttgatca gcgaggcgcc ggaatgcacc acgatattga gttcgccgcc ggtcgccgcg
     3601 cgcaaatcgt cggcgaactg gcgcacgttc tgggtgtggt ggacgccgtc gccgtagggg
     3661 gtaggcatgt cccacttggt ttccgcgaag gccgtggtcg cgcacagcac ggcgccgagc
     3721 gcggctgcgg tgaatagatg tttgccagtc atgtcgattc tcccgtttag ttattatcac
     3781 gcggcgcgtc gaccgtatct ggcgataacg gtccgttggc cgacatccgg gacagtatag
     3841 tcttttcgcg caggcggacc aatcgcaaag cccgtggccc ggcaaataac gggtcgcgga
     3901 agagccatct tcgagtgtct gggggcacga tagacgggcg cccgatacgc gcagttattc
     3961 ctcgcgcaga cttcgaagcc gctgaaatga attgcaggac ccggccaggc ggcgcattgc
     4021 ctgctagctc gctgcgctat tgaactcgtc gatcattcgg tcccaggccg gcaccacctc
     4081 catcagccgg ttccagaatt ccgggtcgcg ccgcgcatcg aaatcttgca gcgatacgcc
     4141 ttgttgctcg acccaggtgt agtagccgag attgaatatc cgctccctgc cgcgcaggct
     4201 caattcctcc atgtgatcgg tggtcgtcgc cagcaggtag cgaccgtaac tttctgccgc
     4261 cgccagctcg tcgaagctgc cgccgaagtc ttgctcggtg gtcctggcga tctcggtgcg
     4321 atacatttcc gcaccatcgg tggccaccgt cagtaccaca tcgtcggcgc ccaaacccag
     4381 gtagcgcgac attttgatgg ccccgaggac gttggcgatc gatgacagcc cgagatcggc
     4441 cagcccgtcg atcagctcac cagaaatccc ggtgcgcttt aacaagaagt cccggccgac
     4501 ggctgtgttg aacaccaggt tcaacgcgtt cgaagccttg tcggtaacgg caaccacgta
     4561 atccgtattc aacacgttat gaatgaatgg cacatgcttg tcgccgatcc cctggatgtt
     4621 atggtcgccg tagccgttgt tcagcagcgt cgggcattcg agcgcttcga cgacgcaaat
     4681 gtcggtgcca agctgcgctt tcagatagtc cccggcagcc agcgtgccgc tggaaccgga
     4741 agccgccacg taggcccgcg cttgcatctt tccattgctg ttgacggcat caaagatgcg
     4801 ctccagggcc ggtccggtga ccgcgcgatg agagatgtaa ttgccgaact cgttgaattg
     4861 gttgaggatt acgttttcgg tatcggtcgc catcaggttg caggcgtcgt agatttcctt
     4921 gacgttgctt tcggtgccat aggtgcggac gatgtcgctc tggtcggtta cccagttttc
     4981 cagccactcg aacctttccc ggctcatgcc ttcgggcaga acggccacgc tgcggcagcc
     5041 gaggatccgg gagatcgcga cgccgccgcg acagtaattc ccggtcgaag gccagacggc
     5101 gcgatgacgg gtcggatcga actgtccgct gatcaagcgt ggcaccagac agccgtaagc
     5161 ggccagtacc ttgtgcgcgc ggatcattgg aaaccgattg ccgatagcca cgactatctt
     5221 tgcatcgaca ccggtcagct ccgggggcag aacgatatgc ccgggcacat ccaccaggtt
     5281 cttgcgatcg gctgcgttgt gccagtgcac gcgaaacagg ttgcgcgcat ccggagagtc
     5341 gggatcggcg gactgtattt gcgactcgat ggcggctcgc gaagagagcg gatcagccag
     5401 ctgagagatc gtcggcagct tgatagcggc ctggcgaaat cgctcgacag cacggtcgta
     5461 gacttcctga ttcacgattt cggtttcgag tttgatcgtc attaatatat atcgcagcgg
     5521 gtttcaatcc gcggcgcctc cgttggctgg attccggttg cgaactgcaa aagcattggt
     5581 gtattcgaat catcgaaaaa cattggctgg cgatggcttg gtttgccctt ggttcgatct
     5641 caacccgtaa ctccgtgctt cggggcctgg tttatctgtc gaattccggg ataaaccgat
     5701 tggacgacgg ccagccagcg aagcaatagt gtagcgataa gctgccgatg tcaaaaaacg
     5761 aaatcgtcag gtcccggtat cgagcctgag ctttgacgat aatcgaaact gataatgtaa
     5821 atcgtattgc tcgtccgcgt gctggtctct gataacggta aaatacccgc ttcgacgcgc
     5881 cattcgaggc gatggcgcgt gtgatcacga tcaccgtttc ggcaaacaaa aagtgaaaaa
     5941 aatcactttt ttcgacggct tctggaaaca gccccacaac ggcggatttc gccaggaatt
     6001 caaaaaaaat cacttttcta acaggcagtt agctcgaagc gcggattgca acaccgctac
     6061 tccagtttcg attcacgacc tttcatccgc gccaaacct
//
LOCUS       DATCZV010000456         6403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_355537, whole genome shotgun sequence.
ACCESSION   DATCZV010000456 DATCZV010000000
VERSION     DATCZV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355537"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VIW27_10205"
     CDS             <1..517
                     /locus_tag="VIW27_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5740088.1"
                     /translation="DLLDIGTGTGRILEILAGRVERGVGVDLSSPMLAMARSNIERGG
                     ASNIHVRHGDMYQLPLEDASVDLAVLHMVLHYSDDPGEAIREAGRVLRPGGRLIVIDF
                     AAHAEEKLREEFQHHRLGFTDSEIQQYFRLVGLDADGEIEQLVGDPLTVKFWQAKQAR
                     KLHLLNPATRS"
     gene            532..1404
                     /locus_tag="VIW27_10210"
     CDS             532..1404
                     /locus_tag="VIW27_10210"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5740089.1"
                     /translation="MTDTAPVRFSYEFFPPRSEPMQNRLWRAMGQLERFGPSFFSMTY
                     GAQGSAREISIDTALAMHRESPVPTAAHLTCCAAPRAQVDAVARGFYDAGIRRIVALR
                     GDQPQGVPPAADEYGSVIELIASLRNFDEIDISVAAYPEPHPQARSALADLQHLQRKF
                     DAGADRAITQYFFDADCFLRFRDRAARLGIDKPIVPGILPVHDIERIKRFSERCGASV
                     PAVYDALFAAVDGRPDAQYRLALELAVELCERLMREGVDCFHFYTLNQTDLCLDISLA
                     LGASLKPPKLIPAA"
     gene            complement(1414..2400)
                     /locus_tag="VIW27_10215"
     CDS             complement(1414..2400)
                     /locus_tag="VIW27_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5740090.1"
                     /translation="MTSRQSFTLDDLKALTAGDTLRLNQVITDNLASEVALINQLSQH
                     IILSGGKRLRPMLVMLTAKACDYRGEDDALLAAVVEFIHTATLLHDDVVDDSDMRRGK
                     PTASTIWGNEAAVLVGDYLYSRAFQMMVRVESMPILALLADTTNTIAQGEVLQLLNIR
                     DPDTSEARYQEVIYAKTACLFEAAARIGALLAGADGGRETALRAYGKHLGTAFQLVDD
                     ALDYSADSGELGKNIGDDLAEGKPTLPLIRALETGDAAQRRLIREAIKAGDAESFDEI
                     SRVIADTGALQYTVEQARAHAELARRSLDAFGASEFRQALMFLADYAVERKY"
     gene            complement(2469..3656)
                     /gene="sat"
                     /locus_tag="VIW27_10220"
     CDS             complement(2469..3656)
                     /gene="sat"
                     /locus_tag="VIW27_10220"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY5740091.1"
                     /translation="MINPVGSNHLTPLYVYDDDQRHALVEEAERLPSVVISSAAAANA
                     VMLGGGYFTPLAGYMNLADALACAENMHCVDGRFFPVPILNLVPSAKAIEGAGRIALR
                     DPNVDGNPVIAIQQVEAIEQVNDAQMELMTEKVYRTLDREHPGVAEFNAQGRVAVSGP
                     IQVLNFSYFATDFPDTFRTAVQIREEIEQRGWQRVVAFQTRNPMHLAHEELCHMAMQR
                     LNCDGLVIHMLLGKLKPGDIPADVRDNAIRTMVQLYFPENSAMVTGYGFDMLYAGPRE
                     AVLHAYFRQNMGASHFIIGRDHAGVGDYYGAFDAQEIFDNEVPQGALDIEIFRADHTA
                     WSKKLDRVVMMNEAPDHTRDDFVLLSGTRVREMLSEGIAPPKEFSRPEVANILIDYYQ
                     SQQ"
     gene            complement(3805..4662)
                     /locus_tag="VIW27_10225"
     CDS             complement(3805..4662)
                     /locus_tag="VIW27_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEY5740092.1"
                     /translation="MFHIRSTHRNSSMRFLLLLLCLQPALLCAAPRVVASIPPLQEIA
                     AAVMGDIGIPGVIIDGHASAHHFALKPSHMERLQRADLVIWVDRNFESGFNRIPEALP
                     ASTTGLELLPVLGTGGDDGHFWYAPALLERSVDLIAGELMRLDPANQQRYNTNARLLA
                     AEISAWRQRIETRFRNRAPVLLTAHDFLRSFAADFEWFEIESIYDRHDAHGGLKDLRR
                     LETKLRERPPACLLTLESELPALAESLADKYRLRIVRVDNSPAGDSAEAALIQRLARL
                     QAAIERCAG"
     gene            4665..5180
                     /locus_tag="VIW27_10230"
     CDS             4665..5180
                     /locus_tag="VIW27_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5740093.1"
                     /translation="MLCYNIMPDKTRHPDIAFREHDHRRCQRQNLSAAARLCADRNLR
                     LTSRRRQVFEILLASHRPMGAYDVLAELNRKWPQERIAPPIVYRALEFLLGEGLIHRI
                     ESKNAFISCVQPGDCRGARFLICRDCDRVAELAGGDGGWLSGADDIGFAVDHSVVEIT
                     GVCAECQQDAG"
     gene            5170..5922
                     /gene="znuC"
                     /locus_tag="VIW27_10235"
     CDS             5170..5922
                     /gene="znuC"
                     /locus_tag="VIW27_10235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HEY5740094.1"
                     /translation="MPAEHLLAAREISYRYAQRLVLDQVSFELERGQITTIIGPNGAG
                     KSTLINIVTGLIAGFDGIVERRAGLRIGYLPQKVHVNTLMPLTVERLLGLTQNPSITD
                     IDRALGQTEVAYLKQRQVRSLSEGELKRVLLARTTLGRPDLIVLDEPTSGVDVTGEIR
                     MYELIGELRDELDCAVLLVSHDLHLVMSQTDQVLCLNQHLCCSGLPESVSRHPEYLAL
                     FGSHAAESLAVYTHDHDHVHDVSGEALDHSHG"
     gene            5915..>6403
                     /locus_tag="VIW27_10240"
     CDS             5915..>6403
                     /locus_tag="VIW27_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY5740095.1"
                     /translation="MAEWLDDYLWRSIAAGMIMVTIAAPMGCLMVWQRLAFLSDTLGH
                     AAVLGVGLGLMLQLLPIVGVLAVALIIVFSLSRVGSFNSALSETTLAIISHTGLAGGI
                     ILVGLLPTRSVNLESILFGDLLATSRADLLQLLATTLVLVVLLVRHWRDFVALSVSRE
                     IAQ"
BASE COUNT         1102 a         2012 c         2082 g         1207 t
ORIGIN      
        1 cgatctgctc gatatcggca ccggcaccgg ccgcatcctg gagattctcg ccggccgcgt
       61 cgagcgcggc gtcggcgtcg atttgagcag cccgatgctg gccatggcgc gcagcaatat
      121 cgaacgcggc ggtgccagta acattcacgt tcggcacggc gacatgtacc agttgccgct
      181 ggaggacgcc agcgtcgatc tcgcggtgct gcacatggtg ttgcactaca gcgacgaccc
      241 gggcgaggcg attcgcgagg cgggcagggt gttacgcccc ggcggccgct tgatcgtgat
      301 cgatttcgct gcccatgccg aggagaaact gcgcgaggaa tttcagcacc accgcctcgg
      361 atttaccgac agtgaaattc agcagtattt cagacttgtc ggcctcgatg ccgatggcga
      421 gatcgaacaa ctggtcggcg acccgttgac ggtcaagttc tggcaagcga agcaggcgcg
      481 aaaactgcac ctgctgaacc ccgcgacgag atcctgaacg aggtaaacca gatgaccgac
      541 acagcgcccg tacgtttttc ctacgagttt ttcccgccgc gcagcgagcc gatgcagaat
      601 cgcctgtggc gcgcgatggg ccagctggag cgtttcgggc cgtccttctt ttcgatgacc
      661 tacggcgccc agggcagcgc gcgcgaaatc agcatcgaca ccgcgctggc catgcatcgc
      721 gaatcgccgg tacctaccgc ggcccatctg acctgttgcg ccgcgccgcg cgcgcaggtc
      781 gatgcggtgg cacgcggatt ctacgatgcc ggtattcggc gtatcgtcgc gctacgaggc
      841 gaccaaccgc agggagtacc gcccgctgcc gacgaatacg gctcggtgat cgagctgatc
      901 gcctcgctgc gcaatttcga cgaaatcgat atcagcgtcg ccgcttatcc cgaaccccat
      961 ccgcaggcgc gcagcgcgct cgccgacctg cagcacctgc agcgcaagtt cgatgccggc
     1021 gccgaccgcg cgattaccca gtatttcttc gacgccgatt gctttctgcg ctttcgcgat
     1081 cgcgccgcgc gcctcggcat cgacaagccg atcgtgccgg gcattctgcc ggtgcacgac
     1141 atcgagagaa tcaagcggtt ttccgagcgt tgcggggcca gtgtgccggc cgtttacgat
     1201 gcactgttcg ctgctgtcga cgggcggccc gacgcgcagt atcggctggc gctggagctg
     1261 gcggtggaac tctgcgagcg gctgatgcgc gagggtgtcg actgcttcca cttctacacc
     1321 ttgaaccaga ccgatctgtg cctcgatatc agtctcgcgc tgggcgcgtc gctgaagccg
     1381 ccaaagctga ttccggccgc ctgagcgctg gtctcagtat ttcctttcga ccgcgtaatc
     1441 ggcgagaaac atcagcgcct gccggaactc gctggcgccg aaggcgtcga ggctgcggcg
     1501 cgccagttcg gcatgcgccc gcgcctgctc gacggtgtat tgcagcgcgc cggtatcggc
     1561 gatcacgcgg ctgatctcgt cgaagctttc ggcatcgccg gccttgatcg cctcgcggat
     1621 cagccggcgc tgtgccgcgt cgccggtctc cagcgcacgg atcagcggca gcgtcggctt
     1681 gccctcggcg agatcgtcgc caatgttctt gcccagttcc ccgctgtcgg cggaatagtc
     1741 gagcgcatcg tcgaccagtt gaaaggcggt accgaggtgt ttgccgtaag cccgtaacgc
     1801 cgtctcccgc ccgccatcgg cgccggccag cagcgcaccg atgcgagccg cggcttcgaa
     1861 caggcaggcc gtcttagcgt agatgacttc ctggtagcgg gcctcgctgg tatcgggatc
     1921 gcgaatattg agcaactgca gcacctcgcc ctgggcgatg gtgttggtgg tatcggcgag
     1981 cagcgcgagg atcggcatcg attcgacgcg caccatcatc tgaaacgcgc gcgagtagag
     2041 gtaatcgccg accagtaccg ccgcctcgtt gccccagatc gtgctcgcgg taggcttgcc
     2101 gcggcgcatg tcggagtcgt cgaccacgtc gtcgtgcagc aatgtggcgg tatgaatgaa
     2161 ttcgaccacc gcggcgagca gcgcgtcgtc ttcgccgcga taatcgcagg ctttcgccgt
     2221 caacatcacc agcatcgggc gcaggcgctt gccgccgctg agaataatgt gctggctgag
     2281 ctggttgatc aacgccacct cggaggcgag gttgtcggtg atcacctggt tcaggcgcag
     2341 cgtatccccg gcggtcagtg ccttgaggtc atccagcgtg aatgattggc gcgatgtcaa
     2401 tgcggcttcg gacatagcgg ggagtatcgc aaagagcgcg attgcgatct agttcggatt
     2461 ggcggtattt actgctgtga ctggtaatag tcgatcagta tgttggctac ctcgggacgc
     2521 gagaactcct tcggcggcgc gatgccctcg ctgagcattt cacgtaccct ggtacccgac
     2581 agcagtacga aatcgtcgcg cgtgtggtcg ggcgcctcgt tcatcatcac cacgcggtcg
     2641 agcttcttcg accaggcggt atgatcggcg cggaaaatct cgatatcgag ggccccctgc
     2701 ggaacctcgt tgtcgaagat ttcctgggca tcgaaagcgc cgtaatagtc gccgacgccg
     2761 gcatgatcgc ggccgatgat gaaatggctg gcgcccatgt tttgccggaa ataggcgtgc
     2821 agcacggctt cgcgcggccc ggcatagagc atgtcgaagc cgtagccggt aaccatcgcg
     2881 ctgttttcgg gaaaataaag ttgaaccatg gtgcgaatcg cgttgtcgcg cacgtcggcc
     2941 ggaatatcac cgggcttgag cttgccgagc agcatgtgaa tgaccaggcc gtcgcaattc
     3001 agccgctgca tcgccatgtg acagagttcc tcgtgggcga gatgcatcgg attgcgggtt
     3061 tgaaaggcaa ccacacgctg ccagccgcgc tgttcgattt cctcgcgaat ctgcaccgcg
     3121 gtgcgaaacg tgtccggaaa atcggtcgcg aagtagctga aattgagcac ctggatcggg
     3181 ccgctgaccg cgacccggcc ctgggcgttg aattcggcga cgccgggatg ctcgcggtcg
     3241 agcgtgcggt aaaccttttc ggtcatcaac tccatttgcg cgtcgttgac ctgctcgatg
     3301 gcctcgacct gctggatcgc gattaccggg ttgccgtcga cgttggggtc gcgcagtgcg
     3361 atgcgcccgg cgccctcgat ggccttcgcg gaaggcacca ggttgagaat cggtaccggg
     3421 aagaaacggc catcgacgca atgcatgttc tcggcgcagg ccagcgcgtc ggccaggttc
     3481 atataaccgg cgagcggcgt gaagtaaccg ccgccgagca tcaccgcgtt ggccgcggcc
     3541 gcggaactga tgactaccga gggcagccgc tcggcttcct cgacgagggc atggcgttgg
     3601 tcgtcgtcgt agacgtacag cggggtcagg tgattcgagc cgacgggatt tatcattgtc
     3661 attcgagccg tgttggggtt gatctcaatg tatcgattgc gcccgttgtt ggcaatcgat
     3721 tctggtatta ccgacataga aagcatttat tcagcgcggc accggcgacc ggtctcggcc
     3781 ggctggccga gcgcaggagt cgcgctagcc ggcgcagcgc tcgatcgcgg cctgcagtcg
     3841 cgcgaggcgc tggatcaggg ccgcttccgc gctgtcgccg gccggcgaat tatcgacccg
     3901 cacgatgcgc agccggtact tgtcggcgag actctcggcg agcgccggca gctcggattc
     3961 gagggtcagc agacaggcgg gaggccgctc gcgtagtttc gtctccagcc gccgcagatc
     4021 cttcagccca ccgtgagcgt cgtgccggtc gtagatcgac tcgatctcga accactcgaa
     4081 gtcggcggcg aaggagcgca ggaaatcgtg cgcggtcaac aacaccggcg cgcggtttct
     4141 gaagcgcgtc tcgatgcgct gccgccaggc gctaatttcc gctgccagca ggcgcgcgtt
     4201 ggtgttgtag cgctgctggt ttgccgggtc gagccgcatc agttcgccgg cgatcaggtc
     4261 gacgctgcgc tcgagcagcg cgggcgcgta ccagaaatga ccgtcatcgc cgccggtccc
     4321 gagtaccggc agcagttcca ggccagtcgt cgacgccggc agcgcctcgg gaatacggtt
     4381 gaatccggac tcgaagttgc ggtcgaccca gatgaccagg tcggcccgct gcaggcgctc
     4441 catgtgcgac ggcttgagcg cgaaatgatg cgccgacgcg tgaccgtcga taattacccc
     4501 gggtatgccg atatcgccca tgaccgcggc ggcgatttcc tgcagcggcg ggatagaggc
     4561 gaccacgcgc ggcgcggcgc acagcagtgc gggctgcagg caaagtagta acagcaggaa
     4621 gcgcatcgac gagttccggt gagtgctgcg aatgtggaac aactatgtta tgttataaca
     4681 ttatgcccga caagacccgc catcccgata tcgcgtttcg cgagcacgat caccgtcgct
     4741 gccagcggca aaatctgagc gccgcggcca ggctgtgcgc agaccgcaac ctgcgcctga
     4801 cctcacgccg ccggcaggtg ttcgaaattc tgctggcgag ccaccggccg atgggcgctt
     4861 acgacgtgct tgccgagttg aaccgcaagt ggccgcagga acgcatcgca ccgccgatcg
     4921 tctaccgtgc gctcgaattt ttgctcggcg agggcctgat tcaccgaatc gagagcaaga
     4981 acgcgttcat ttcctgcgtg caacccggcg attgccgcgg cgcgcgcttc ctgatttgcc
     5041 gcgattgcga ccgggtcgca gagctcgcgg gcggcgacgg cggctggctc agcggtgccg
     5101 acgatatcgg cttcgccgtc gatcactcgg tggtcgagat caccggggtt tgtgccgagt
     5161 gccagcaaga tgccggctga acatctgctc gcggcccgcg aaatcagcta tcggtacgcg
     5221 cagcgcctgg tgctcgacca ggtcagcttc gaactcgagc gcggacagat caccaccatc
     5281 atcggcccca acggcgccgg taaatcgacc ctgatcaata tcgtcaccgg attgatcgcg
     5341 gggttcgacg gcatcgtcga gcgccgggcc gggctgcgta tcggctacct gccgcaaaag
     5401 gtccatgtca acacgctgat gccgctgacc gtcgagcggc tgctggggct gacgcaaaac
     5461 ccgtccatca ccgacatcga ccgggcgctc gggcaaaccg aggtggccta tctgaaacag
     5521 cgccaggtgc gctcgctgtc cgagggcgag ctcaagcgcg tgctgctggc gcgcaccacg
     5581 ctcggccgtc ccgacctgat cgtgctcgac gagccgacct cgggggtcga cgtgaccggc
     5641 gaaatccgca tgtacgaact gatcggcgag ctgcgtgacg agctcgattg cgcggtgttg
     5701 ctggtctcgc acgacctgca cctggtgatg tcgcaaaccg accaggtgct gtgcctgaac
     5761 cagcacctgt gctgctcggg gctgcccgaa tcggtgagcc ggcaccccga gtacctggcg
     5821 ctgttcggca gccatgccgc ggagtcgctc gccgtttata cccacgatca cgatcacgtg
     5881 cacgacgtct ccggcgaagc cctggatcac tcgcatggct gaatggctgg acgattacct
     5941 gtggcgcagc attgctgccg gaatgatcat ggtgacgatc gccgcgccga tggggtgcct
     6001 gatggtctgg cagcggctcg ccttcctcag cgataccctc ggccatgcgg ccgtgctcgg
     6061 tgtcggtctc ggcctgatgc tgcagctgct gccgatagtc ggggtgctgg cggtggcgct
     6121 gatcatcgtt ttttccctga gccgtgtcgg cagtttcaac agcgcgctgt cggaaaccac
     6181 gctggcgatc atttcgcata ccgggctcgc cggcggcatc attctggtgg gcctgctgcc
     6241 gacgcgcagc gtcaacctcg agagtatcct gttcggcgat ctgctcgcca cctcgcgcgc
     6301 cgacctgctg cagctgctgg cgaccacact ggtgctggtg gtgctgctgg ttcgccactg
     6361 gcgcgacttc gttgcgctgt cggtgagccg cgagatcgcg cag
//
LOCUS       DATCZV010000457         4625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_90347, whole genome shotgun sequence.
ACCESSION   DATCZV010000457 DATCZV010000000
VERSION     DATCZV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90347"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VIW27_10245"
     CDS             complement(<1..444)
                     /locus_tag="VIW27_10245"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY5740096.1"
                     /translation="MLDTRYRVEIPGGIHLEAQVVGPIPRCFAFVIDLFIRGLLMFAL
                     SLASLPFGIFGLGGGFLLILLFMLEWLYPVLFEVFWRGQTPGKRILGIAVINDDLTPV
                     TLGTSLVRNLLRTVDFLPMYYLAGLVTMLANRRFQRLGDLAAGTLV"
     gene            complement(642..1397)
                     /locus_tag="VIW27_10250"
     CDS             complement(642..1397)
                     /locus_tag="VIW27_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740097.1"
                     /translation="MSDIYATPEADLSQNIELDRAGGNIDDAVAGNFQVNMLETLGEA
                     WRGLKGFKLKCHLALILYFLVFLGATIVYGIAVTMIVSSGADQSVIGVMSMVFQLVIT
                     VVSLPMTYALLIMAMRHAHDKSVSAGEVFRHFGAVLSLFVAYVLEVILIMIGLILLVL
                     PGIYLMFAYMYAMPLIIEKKMGPWHAMEVSRRALTKVWFRFFGLMWLIMLINMLGMLT
                     LGIAWIWTIPWSILAMALVYEKIFGVEAHTLAD"
     gene            complement(1503..3602)
                     /locus_tag="VIW27_10255"
     CDS             complement(1503..3602)
                     /locus_tag="VIW27_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5740098.1"
                     /translation="MKHWLERLLSPRTIAIVGASERDGSIAANTHRQLRERGFGGRIY
                     SVNPRYPSLHGDVCHATLAALPEVPDLVLYAISGLPLEQSFTEAVALGVGGVLIYAAN
                     HVDGDTDPPLTERLRQQAAAAGIPVCGGNSMGFYNYDRDIMVSFDRPPPQRPRGHIGL
                     ISHSGSGMTYLANNDTRFCFNYVIASGQEIHASVGHYIDYLLQQESTRVIAILLEAVR
                     DVPAFTAALERAGERSIPVVITRLGRTAKSAQLARSHSGAIVGDQAAFAALCRRHGAI
                     LCRDADEMMVTAMLFAAGLRVTEGKLASMLDSGGMREQMIDLADEIGVEFADVSPVTV
                     AVMRAHLEAGLEAINPMDGMGALGRNSLQTFLACGRALLDDVQTGLLSFEFEFRDGFS
                     HYPELFDVTRQLAAHNDKPVVLINSCAFATLDETAARLTGDGIPVINGIDVALRGLRN
                     LMYYRPAKALAVSPPSMPVFERERVDRWRQRLCTESGFDEVDALGLMADFGLPAVEFV
                     LADSLDELQQAVENFGWPLVLKTAAAGIAHKTEQGGVRVGIGNAAELEAAYRDISARL
                     GPRVVAMPMVAAGVEAALGMKNDPQYGPLVIVGCGGVLIELLAERALRLAPVAAGEAA
                     AMIDETRLACLLAGVRGQAPADRCALEALLVRFSALVLEFADCIGEIDLNPVIVNRDG
                     CRIVDALVVPEAMQQGE"
     gene            3679..4485
                     /locus_tag="VIW27_10260"
     CDS             3679..4485
                     /locus_tag="VIW27_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5740099.1"
                     /translation="MSDAVRLHNHGRILEITIDRPPANAINPGVSKAVHAALSRLQED
                     ASLQVGILTGGGERFFSAGWDLKEIAAYDNQQAAVASAFEGPGGFGGICEFWGLKKPV
                     IAAVNGMAAGGGFEISLACDLIVAAEHAEFFLPDLQRGFLPDVGAVQLLPRKLPYNLA
                     MELLYTGRRLGAEEAHRHGLVSRVCPRDRLLDAARDLAAEIAKSAPLALQALKEVVPA
                     IRELPLPAAFAATKPGNANLPIYQRMLESEDFMEGPRAFADKREPLWKGR"
BASE COUNT          942 a         1499 c         1413 g          771 t
ORIGIN      
        1 aaccagcgtg ccggcggcca ggtcgccgag ccgctgaaaa cgtcgattgg ctagcatcgt
       61 caccagcccg gcgaggtagt acatcggcag gaaatccacc gtgcgcaaca ggttgcgcac
      121 cagcgatgta ccgagagtaa ccggcgtcag gtcgtcattg attacggcga tgccgagaat
      181 gcgcttgccc ggtgtctggc cgcgccagaa aacctcgaac agcaccggat aaagccattc
      241 gagcatgaac agcaggatca gcagaaagcc gccgccgaga ccgaagatgc cgaacggcag
      301 cgacgccagc gacagcgcga acatcagcag accgcgaatg aacaagtcga tgacaaacgc
      361 gaagcagcgc ggaatcggcc cgaccacctg cgcttcgaga tgaattccgc ccggaatctc
      421 gacccggtag cgtgtatcca gcaatcaact attccctggc cggaaaatca cgccgccata
      481 ccgagcgccc cgacccggct gcgcggcaca ggacagccac ccgcgagctt cacagcgaac
      541 cggcaacgat ggcttatgca cttcttgctc agtgattgaa aacacgatat gcctgttaat
      601 ttccagttac gggcatcagc ccggccggcg caaccggcga gctagtccgc cagggtatgc
      661 gcctcgacac cgaaaatctt ttcgtagacc agcgccattg ccagtatcga ccacggaatg
      721 gtccagatcc aggcgatgcc cagggtcaac atgccgagca tattgatcag catgatcaac
      781 cacatcaggc cgaaaaaacg aaaccagact ttggtcagcg cacggcgcga gacttccatc
      841 gcgtgccacg ggcccatttt cttttcgatg atcagcggca tagcgtacat gtaggcaaac
      901 atcaggtaaa tgcccggcag caccaacaat atcagtccga tcatgatcag gatcacttcc
      961 agtacatagg ccacgaacag gctgaggacg gcaccgaaat gccggaacac ctcgccggca
     1021 gagaccgact tgtcgtgcgc atgccgcatc gccatgatca acagcgcgta ggtcatcggc
     1081 agcgagacca cggtgattac cagttgaaaa accatgctca tcacgccgat gacactctgg
     1141 tcggcaccgc tggagacgat catcgtcacc gcgatgccgt agacaatggt ggcaccaaga
     1201 aataccagga aataaaggat cagcgcaagg tgacacttca gcttgaagcc tttcaatcct
     1261 cgccatgcct cgcccagggt ttccagcatg ttgacctgga aattgccggc gacggcatca
     1321 tcgatattgc ctccggcgcg atccagttct atgttctgcg acagatcggc ctcgggtgtt
     1381 gcatagatat cagacatgtc tgactccacg atggtttacc tgcaagccaa tcgattatag
     1441 caggatgtat cgcgaacgcg gcgcgcgcgg cagccgcgta tggtgttagc ccggcacttt
     1501 cgctattcgc cttgttgcat ggcctcaggc actaccagcg catcgacgat gcggcagccg
     1561 tcgcggttga cgattaccgg gttgagatcg atctcgccga tgcaatccgc gaactcgagc
     1621 accagcgccg agaaacgcac cagcagagcc tccagcgcgc agcgatcggc cggcgcctgg
     1681 ccgcgcacgc cggccagcag gcacgcgaga cgggtttcgt cgatcatcgc cgccgcctcg
     1741 ccggcagcga ccggcgccag ccgcagcgcc cgctcggcga gcagctcgat cagcactccg
     1801 ccgcagccga cgatcaccag cggcccgtac tgcggatcgt ttttcatccc cagcgccgcc
     1861 tcgacgccgg cggcgaccat cggcatcgcg actacccgcg gaccgaggcg cgcggagata
     1921 tcgcggtaag ccgcctcgag ttcggcggca ttgccgatgc cgaccctgac accgccctgt
     1981 tcggtcttgt gcgcaatgcc cgcggcagcg gttttcaaca ccagtggcca gccgaaattc
     2041 tcgaccgcct gctgcagttc gtcgagtgag tcggcgagca cgaattcgac cgcgggcaat
     2101 ccgaagtccg ccatcaggcc gagcgcgtcg acctcgtcga aaccgctttc ggtgcacagg
     2161 cgctggcgcc agcggtcgac gcgttcacgc tcgaaaaccg gcatcgacgg cggcgatacg
     2221 gccagcgctt ttgcgggccg gtaatacatc aggttgcgca gcccgcgcag cgcgacgtcg
     2281 atgccgttga tcaccggaat gccgtcgccg gtcagccgcg ccgcggtctc gtccagcgtc
     2341 gcaaaagcac aactgttgat cagcaccacc ggcttgtcgt tgtgcgcggc gagctgtcgg
     2401 gtaacgtcga acaactccgg gtaatgcgaa aagccgtcgc ggaactcgaa ttcgaagctc
     2461 agcaaaccgg tctgcacatc gtcgagcagc gcgcggccac aggcgagaaa ggtctgcagc
     2521 gaattacgcc ccagtgcgcc catgccgtcc atcgggttga ttgcctcgag gcccgcttcc
     2581 aggtgagcac gcatcaccgc gacagtaacc ggcgagacgt cggcgaactc gacgccgatt
     2641 tcgtcggcga ggtcgatcat ctgctcgcgc ataccgcccg aatcgagcat gctggcgagc
     2701 ttgccctcgg tgacgcgcaa gccggccgcg aacagcattg cggtaaccat catctcgtcg
     2761 gcatcgcggc acaggatcgc gccgtgacgc ctgcacagcg cggcaaacgc cgcttggtcg
     2821 ccgacgatcg cccccgagtg gctgcgcgcc agctgcgccg attttgcagt gcgcccgaga
     2881 cgcgtgatca ccaccgggat cgagcgttcg ccggcgcgct ccagcgcggc ggtaaatgcg
     2941 ggcacatcgc gtaccgcttc cagcaggatc gcgatcaccc gtgtcgattc ctgttgcagc
     3001 aggtaatcga tgtaatggcc gacgctggcg tggatttcct ggccgctggc aatgacgtaa
     3061 ttgaagcaga agcgagtgtc gttattggcg aggtaggtca tgcccgaacc ggaatgcgaa
     3121 atcagcccga tgtggccgcg cgggcgctgc ggcggcggac ggtcgaagct gaccatgata
     3181 tcccggtcgt agttgtagaa gcccatcgag ttgccgccgc agaccggaat gccggcggcg
     3241 gcggcctgtt gccgcagacg ctcggtcaac ggcggatcag tatcgccgtc gacatggttg
     3301 gccgcgtaga tcagcacacc gccgacgccg agcgcgactg cctcggtaaa gctttgctcg
     3361 agcggcaggc cgctgatcgc gtagagcacc aggtcgggta cttcgggcaa ggcggcgagc
     3421 gtggcgtggc agacatcacc atgcagggac ggataacgcg gattgaccga gtaaatgcgg
     3481 ccgccgaaac cgcgctcgcg cagctgccgg tgggtgttag cggcgatact gccgtcacgc
     3541 tccgaggcgc cgacgatggc gatcgttcgc ggtgacaaca agcgctccag ccagtgcttc
     3601 atcgaatcgg gccaaacagt gattgcggcg cgattttgac atatattgcc ggcctgcaca
     3661 caccatttag aggtcagcat gagcgacgca gtcaggctac acaaccacgg ccggattctc
     3721 gagatcacca tcgaccggcc gcccgccaac gcgatcaatc cgggcgtctc aaaggcggtc
     3781 cacgcggcct tgtcgcggct gcaggaagac gcttcgctgc aggtcggtat tctcaccggc
     3841 ggcggcgagc gcttcttcag cgccggttgg gatctgaagg aaatcgctgc ctatgacaat
     3901 cagcaggcag cggtggccag cgccttcgaa ggacccggcg gcttcggcgg catttgcgaa
     3961 ttctggggac tgaagaaacc ggtgatcgcc gcggtcaacg gcatggcggc cggcggtggt
     4021 ttcgagattt cgctggcctg cgacctgatc gtcgccgccg agcacgccga gtttttcctg
     4081 cccgatctgc agcgcggctt tctgcccgac gtcggcgcgg tgcaactcct gccacgcaag
     4141 ctgccctaca acctggccat ggaactgcta tataccgggc gccggctcgg tgccgaggaa
     4201 gctcaccggc acggcctcgt cagccgggtc tgtccgcgcg accgcctgtt ggacgcagcg
     4261 cgagatcttg ccgccgaaat cgccaagtcg gcaccactgg cgctgcaggc cctgaaggaa
     4321 gtggtgccgg cgattcggga actgccgctg ccggcggcat tcgcggccac caaacccggc
     4381 aatgcgaatt tgccgatcta ccagcgcatg ctcgaatcgg aagatttcat ggaaggcccg
     4441 cgcgcgttcg ccgacaagcg cgaaccgctc tggaagggcc gttagcttcc gtcaagccgt
     4501 cgtcaggtcg cggcgcggcc gccattgtcg cctgccgtgc tagccgcctg aaccctcggc
     4561 cacggcgcct ggtgctacct tatcgcgcag ttcacgcagc cgccactgga tcgagatgca
     4621 gtaca
//
LOCUS       DATCZV010000458         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_620575, whole genome shotgun sequence.
ACCESSION   DATCZV010000458 DATCZV010000000
VERSION     DATCZV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620575"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VIW27_10265"
     CDS             <1..256
                     /locus_tag="VIW27_10265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY5740100.1"
                     /translation="DYIYEPGSKEVLDQVLDRYIESLVYQGVVENVACEMAARMIAMK
                     NATDNAGAMIDELQLIYNRERQASITQEISEIVGGAAALK"
     gene            284..1663
                     /gene="atpD"
                     /locus_tag="VIW27_10270"
     CDS             284..1663
                     /gene="atpD"
                     /locus_tag="VIW27_10270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5740101.1"
                     /translation="MSSGKIVEVIGAVVDVEFPRDSVPKIYDALLISEAEGLTLEVQQ
                     QLGDGIVRTIAMGAAEGLRRGYEVTNTGEPITVPVGQKTLGRIMDVLGNPIDEKGPIG
                     EESRMPIHRTPPTYDEQAATTEILETGIKVIDLVMPIAKGGKIGLFGGAGVGKTVTLM
                     ELIRNIAVEHSGFSVFAGVGERTREGNDFYHEMTDGGVIDKVALVYGQMNEPPGNRLR
                     VALSGLTMAEYFRDEGRDVLMFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLALEMG
                     VLQERITSTKTGSITSFQAVYVPADDLTDPSPATTFAHLDATLVLSRQVAELGIYPAV
                     DPLDSSSRILDPNVVGNEHYDVARGVQGTLQRYKELKDIIAILGMDELSEEDKLVVSR
                     ARKIQRFLSQPFFVAEVFTGSPGKYVSVKDTISSFKQILDGEMDQYPEQAFYMVGGIE
                     EVAEKAKNL"
     gene            1673..2086
                     /locus_tag="VIW27_10275"
     CDS             1673..2086
                     /locus_tag="VIW27_10275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY5740102.1"
                     /translation="MSTIQVEIVSAEESIFSGEAQMVYAPAVMGEVGIAPRHTPLISP
                     LKPGEVRLDMGDGKEEFFFVSGGILEVQPHMVTVLSDTAIRARDLDEAAALDAQKRAE
                     AALVDQRSDLDVAKAKAELAAAAAQIAAIKKLRRK"
BASE COUNT          451 a          671 c          720 g          384 t
ORIGIN      
        1 ggattacatc tacgagcccg gttcgaaaga agtgctcgat caggtgctcg accgctacat
       61 cgaatcgctg gtttaccagg gcgtggttga aaacgtggcc tgcgagatgg cggcgcggat
      121 gatcgcgatg aagaacgcca ccgacaatgc cggcgcgatg atcgacgaat tgcaattgat
      181 atacaaccgc gagcgccagg cctcgatcac gcaggagatc agcgaaatcg tcggcggtgc
      241 ggcggcactt aaataattta ggattcatta cgaggtttga cagatgagct caggtaaaat
      301 cgtggaagta atcggcgcgg tcgtcgatgt ggagtttccg agggactcgg ttcccaagat
      361 ctacgacgcg ctgctgatct ccgaggccga gggcctgacg ctggaagtcc agcagcagct
      421 cggcgacggc atcgtgcgca ccatcgcgat gggtgccgcc gagggcttgc gccgcggcta
      481 cgaggtgacc aacaccggcg agccgatcac cgtgccggtg gggcagaaga ccctgggccg
      541 catcatggat gtgctcggca atccgatcga cgagaaaggc ccgatcggcg aggaatcgcg
      601 catgccgatc caccgcacgc cgccgaccta cgacgagcag gcggcgacca ccgaaattct
      661 cgagaccggc atcaaggtca tcgacctggt catgccgatc gccaagggcg gcaagatcgg
      721 cctgttcggc ggcgccggcg tgggcaagac ggttaccctg atggagctga tccgcaacat
      781 cgcggtcgag cactcgggct tctcggtatt tgccggcgtg ggcgagcgga ctcgtgaagg
      841 caacgacttc taccatgaaa tgaccgatgg cggcgttatc gacaaggtgg cgctggttta
      901 cggccagatg aacgagccgc cgggcaaccg cctgcgcgtg gcgctgtcgg gcctgacgat
      961 ggccgagtac ttccgcgacg agggccgcga cgtactgatg ttcgtcgata acatttaccg
     1021 ttacacgctg gccggcacgg aagtttccgc actgctcggt cgcatgccgt cggcggtagg
     1081 ttaccagccg accctggcac tcgagatggg tgtgctgcag gagcgcatca cgtcgaccaa
     1141 gaccggatcg atcacgtcct tccaggcggt atacgtgccg gcggacgacc tgactgaccc
     1201 gtcgccggcc accaccttcg cgcacctcga cgcgacgctg gtactgtcgc gccaggtggc
     1261 ggaactgggt atctacccgg cggtggaccc gctcgactcg agctcgcgga tcctcgatcc
     1321 gaacgtggtc ggcaacgagc actacgacgt cgcccgcggc gtacagggta cgctgcagcg
     1381 ctacaaggag ctgaaagaca tcatcgccat tctcggcatg gacgaactct ccgaggaaga
     1441 caagctggtg gtcagccgcg cgcgcaagat ccagcgcttc ctgtcgcagc cgttcttcgt
     1501 cgccgaggta tttaccggtt cgccgggcaa gtacgtatcc gtcaaggaca ccatctcgag
     1561 cttcaagcag atcctcgacg gcgagatgga ccagtacccg gagcaggcgt tttacatggt
     1621 cggcggcatc gaagaagtcg ccgaaaaggc caagaacctg taggctagcg atatgagcac
     1681 gatccaggtc gaaatcgtca gcgccgagga gtcgatcttc tcgggcgaag cgcagatggt
     1741 ctatgcgccc gcggtgatgg gcgaggtcgg catcgcgccg cgccacacgc cgctgatctc
     1801 gccgctgaag ccgggcgagg tgcgcctcga catgggcgac ggcaaggaag agttcttctt
     1861 cgtctccggc ggcatcctcg aggtgcagcc gcacatggtg acggtattgt ccgacaccgc
     1921 gattcgtgcc cgcgacctcg acgaggcggc cgcgctggat gcgcagaagc gcgccgaagc
     1981 cgcactcgtc gatcagcgct cggacctcga cgtggccaaa gccaaggccg aactggcggc
     2041 ggctgccgcg cagattgcgg cgatcaagaa actcagaagg aagtgaccga tctgatccag
     2101 gtgaaagaaa atcgggcgtt gtcccgattt tttttgcctt ctcaataagc gcgaaagccg
     2161 ccctccagcc ggcggctggt accctgacct caaacggtct gccagccggg tttccgctcg
     2221 attttt
//
LOCUS       DATCZV010000459         7006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_673224, whole genome shotgun sequence.
ACCESSION   DATCZV010000459 DATCZV010000000
VERSION     DATCZV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673224"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VIW27_10280"
     CDS             complement(<1..297)
                     /locus_tag="VIW27_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5740103.1"
                     /translation="MNKTVNPKAWWFVVPVLALVAFNAFIPLMTVVNYAFQETFGNNV
                     FFWEGVKWYEQVLHSERFHASLLRQLAFTAIILVIEVPLGVAIALTMPRKGPWVS"
     gene            complement(294..1370)
                     /locus_tag="VIW27_10285"
     CDS             complement(294..1370)
                     /locus_tag="VIW27_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008887030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740104.1"
                     /translation="MARIEFKQVRHAYPAPPGQPRVYALKEVNQVWEDGGAYALLGPS
                     GCGKTTLLNAISGLLTPSEGSILFDGEDITQLPTAQRRIAQVFQFPVVYDTMTVYDNL
                     AFPLRNRGTAEDVVRQRVEEIAAMLDLSDRLRQRAAGLTADGKQKISLGRGLVREDVN
                     AILFDEPLTVIDPHLMGKLRSKLKYLHQQMGVTMIYVTHDQVEALTFADKVVVMYDGT
                     VVQVGTPVELFNRPNHTFVGYFIGSPGMNVLPCSLDGGKPVFHGHPLATNHPADGQLD
                     GKLEVGVRPEFVHFSDDGIPVRIEKVEDLGRYQVVTAKHETDTIKMVVGEDERVPTEN
                     PQIAFDPEFTRIYRDDWVVGGPAS"
     gene            complement(1384..2448)
                     /locus_tag="VIW27_10290"
     CDS             complement(1384..2448)
                     /locus_tag="VIW27_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740105.1"
                     /translation="MAVELRGVSLRVGADTHVYETSLALEPGQFNILLGTTLAGKTTL
                     MRLMAGLEKPTTGEVWAGGKNVTGVAVQKRSVAMVYQAFINYPSFSVYDNIASPLKVA
                     GLPRADIDRQVRRFAELLQLTPMLERLPSELSGGQQQRTALARALVKGADLVLLDEPL
                     ANLDYKLREELRDELPKLFADTGATVVYATSEPLEALMLGGFTATLKEGRVVQFGATS
                     DIYRHPETLESAEVFSDPPVNTASIAKRGERAFLSDAVSWELPGRVRELPDGDYKIAL
                     RPHHLLPDGDGTGVEVDGEVQIAEISGSESIVRVNIAGNNWVSESHGIHSYEYGARAR
                     FRFDPSRCFYFDADEKLIAS"
     gene            complement(2449..3252)
                     /locus_tag="VIW27_10295"
     CDS             complement(2449..3252)
                     /locus_tag="VIW27_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY5740106.1"
                     /translation="MNVEISSRQHLIMDYIREHGAVQVDQLSAHLQVTPQTIRRDLNQ
                     LYELKLVQRVHGGAIVKDNVENLGYGARKILMAEEKTDIARRAAELIPDNSSLFINIG
                     TTTERVAEHLHERSGLLVITNNINVAGMLWPVRGIEVMIAGGLIRRSDGGIVGASAEE
                     FIAKFKLDYAVIGCSAIDEDGDFFDYDLREVRVTQAIIRHSRTVIMVTDSTKFQRRAP
                     IRIGNLSEVGTLVTDDGLPRESVQYCRNNRIDLLIVGQDDQRRPAGGEL"
     gene            complement(3353..5080)
                     /locus_tag="VIW27_10300"
     CDS             complement(3353..5080)
                     /locus_tag="VIW27_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740107.1"
                     /translation="MFKQIAAAATLAALSTQPVFAGMAEAERWVDDEFQPSSLSRADQ
                     LKEMQWFIDAAKPFAGMEINVLSETIPTHEYESKTLTKAFEEITGIKVNHQLLGEGEV
                     VQAVQTQMQTKRNLYDAYINDSDLIGTHSRLQLAINLSDWMAGEGKAVTNPMLDVDDF
                     IGKSFTTGPDGKLYQLPDQQFANLYWFRKDWFDRADLQQRFKAKYGYDLGVPVNWSAY
                     EDIAEFFSEDVKEIDGVRVYGHMDYGKRAPDLGWRMTDAWLSMAGAGSKGLPNGVPVD
                     EWGIRMESGSCNPVGASVTRGGAANGPASVYAIRKWDEWLRKYAPPGAASYDFYQSLP
                     ALSQGNVAQQIFWYTAFTASMVAPKSDGNNTVDDNGKPLWRMAPSPHGPYWEEGQKLG
                     YQDAGSWTLFKSTPVDRRKAAWLYAQFVTSKTVDVKKSHVGLTFIRDSTIRHQSFTDR
                     APNLGGLVEFYRSPDRVLWSPTGINVPDYPKLAQIWWQQIGDVNSGAFTPQQAMDRLA
                     SEMDLVMSRMQAADEKANTYGGCGPRLNEEKEASYWLNQPGSPKAKVNEKPKGQTVNY
                     EELVKRWKQ"
     gene            5289..5759
                     /locus_tag="VIW27_10305"
     CDS             5289..5759
                     /locus_tag="VIW27_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5740108.1"
                     /translation="MAETRQLLIVAHNPSPNTQKLVAATLRGARHPDIEGVEARHVPP
                     LEAVVDDVLGADAIILGTTENFGYMSGALKDFFDRIYYPCLEHTEAMPFATWIRAGLD
                     GTGTKVAIGKITSGLKWKAVQQPLILHGEYRTEFEDQCEELGMLMAASLESGII"
     gene            6051..6746
                     /locus_tag="VIW27_10310"
     CDS             6051..6746
                     /locus_tag="VIW27_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020053100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5740109.1"
                     /translation="MNKFQGKKAIVLGGTSGIGLAATEMLRDGGAEVIAGGRRECEID
                     GVETARIDVLDRDGLQALFEKHQGFDYLVNAATGGPRAIGPFLEMDLDGFQGSFAKLW
                     GYVNSVRLGVPLMSKQGAVVLFSGYPARKYRPGTLAIGSVGGSVEAFIQLVANEIAPV
                     RINGVCPGLIDTPMSPLEGEARRNYYADGTRNHLIPRAGTPAECAGAAIFLLENEFVT
                     GTIVDVDGGAICT"
     gene            6743..>7006
                     /locus_tag="VIW27_10315"
     CDS             6743..>7006
                     /locus_tag="VIW27_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEY5740110.1"
                     /translation="MSCEKICRSLAGDTPGRSTELNYFRIGPAQADRKVYLQAALHCD
                     EQPGILILHHLLQLLREADEHDQLAAQFVLFPMVNPLGMSDIQF"
BASE COUNT         1230 a         2151 c         2227 g         1398 t
ORIGIN      
        1 cgagacccag gggcccttgc ggggcatggt cagcgcgatg gcaaccccga gcggcacctc
       61 gatcaccagg atgatggccg tgaaggcgag ctggcgcagc agcgaggcgt ggaagcgctc
      121 ggaatggagc acctgctcgt accacttcac cccctcccag aagaagacgt tgttgccgaa
      181 ggtctcctgg aacgcgtagt tgaccaccgt catcagcgga atgaaagcgt tgaaggcgac
      241 cagcgccagc accggcacca cgaaccacca ggctttcggg ttgacggtct tgttcacgat
      301 gccggccccc cgacgaccca gtcgtcccgg taaatacggg tgaattccgg gtcgaaggcg
      361 atttgcgggt tctccgtcgg cacccgctcg tcctcgccga ccaccatctt gatggtatcc
      421 gtctcgtgct ttgcggtcac cacctggtaa cgcccgagat cctcgacctt ttcgatacgc
      481 accggaattc cgtcatcgct aaagtgcacg aattcgggtc gcacgccgac ctcgagcttg
      541 ccgtcgagct ggccgtccgc ggggtggttg gtcgccagcg gatggccgtg aaacacgggc
      601 ttgccgccgt cgaggctgca cggcagcacg ttcatgccgg gcgagccgat gaagtagccg
      661 acgaaggtat ggttgggccg attgaacagt tccaccggcg taccgacctg caccacggtg
      721 ccgtcgtaca tcaccaccac cttgtcggcg aaggtcagcg cctcgacctg gtcgtgggtg
      781 acgtaaatca tcgtcacccc catctgctgg tgcaaatact ttagcttgga gcgcaatttc
      841 cccataaggt gcgggtcgat gaccgtcagc ggttcgtcga acaggatcgc gttgacgtct
      901 tcgcgcacca ggccgcggcc gagcgaaatc ttttgcttgc cgtcggcggt aaggccggcg
      961 gcgcgctgcc gcaggcggtc ggacagatcg agcatcgcgg cgatttcctc gacccgttgc
     1021 ctgaccacgt cctcggcggt gccgcggttg cgcagcggga aggcgagatt gtcgtagacc
     1081 gtcatcgtgt cgtagaccac cgggaactgg aacacctgcg cgatacggcg ctgcgcggtc
     1141 ggtagctggg tgatgtcttc gccgtcgaac aggatcgagc cctcggacgg cgtcagcagg
     1201 cccgagatcg cgttcagcag cgtggtcttg ccgcagcccg acggccccag cagcgcatag
     1261 gcgccgccgt cttcccacac ctggttgact tccttgagcg cgtagacccg cggctgcccg
     1321 ggtggcgccg ggtaagcgtg cctgacttgc ttgaattcga tcctggccat ttgcgctacc
     1381 cgttcacgaa gcgatgagtt tttcgtcggc gtcgaaatag aagcagcgcg acggatcgaa
     1441 acgaaagcgc gcccgcgcgc cgtactcgta gctgtggatg ccgtgcgact cgctgaccca
     1501 gttgttgccg gcgatgttga cgcgcacgat gctctcggag cccgagatct cggcgatctg
     1561 tacctcgccg tcgacctcga caccggtgcc gtcgccgtcg ggcagcaggt gatgcggacg
     1621 cagcgcgatc ttgtaatcgc cgtcgggcag ttcgcgcacc cggccgggca attcccagct
     1681 caccgcgtcg ctgagaaagg cgcgctcgcc gcgcttggcg atggacgcgg tattgaccgg
     1741 cggatcggag aaaacctcgg cgctttcgag tgtctcggga tggcggtaaa tatccgaggt
     1801 ggcgccgaac tgtaccacgc gcccttcctt gagggtcgcg gtaaagccgc ccagcatcaa
     1861 tgcctcgagc ggctcgctgg tggcgtagac cacggtcgcg ccggtgtcgg cgaacagctt
     1921 cggcagctcg tcgcgcaatt cctcacgcag cttgtagtcg aggttggcca gcggctcgtc
     1981 gagcagcacc aggtcggcgc ccttgaccag cgcgcgcgcc agcgccgtgc gctgctgctg
     2041 gccgccggac agctcgcttg gcagccgctc gagcatcggc gtcagttgca gcagttccgc
     2101 gaagcgcctg acctggcgat cgatatccgc cctcggcagg ccggcgacct tcagcggcga
     2161 ggcgatattg tcgtagacgc tgaagctcgg gtaattgatg aaagcctggt aaaccatcgc
     2221 cacgctgcgt ttttgtaccg ccacgccggt gacattctta ccgcccgccc agacttcgcc
     2281 ggtggtcggt ttttcgaggc ccgccatcag ccgcatcagt gtggtcttgc cggccagcgt
     2341 ggtgccgagc aggatgttga actggcccgg ctcgagtgcg aggctggtct cgtaaacgtg
     2401 ggtgtcggca cccacccgca gcgatacacc gcgcagttcg acggccatct acagttctcc
     2461 tccggccggc cggcgctgat cgtcttggcc gacaatcagc aggtcgatgc ggttattgcg
     2521 gcaatactgc acgctttctc gcggcaggcc gtcgtcggtg accagcgtgc cgacctccga
     2581 caggttgccg atgcgaatcg gcgcgcggcg ttggaacttg gtcgagtcgg tgaccatgat
     2641 caccgtgcgg gagtgacgaa tgatcgcctg ggtgacacgc acctcgcgca ggtcgtaatc
     2701 gaagaaatcg ccgtcctcgt cgatcgccga gcagccgatc actgcgtagt cgagcttgaa
     2761 cttggcgatg aactcctcgg cgctggcgcc gacgataccg ccgtcggagc ggcgaatcag
     2821 gccgccggcg atcatcacct cgatgccgcg gaccggccac agcatgccgg cgacgttgat
     2881 gttattggtg atcaccagca ggccgctgcg ctcgtgaaga tgctcggcga cgcgctcggt
     2941 ggtggtaccg atattgatga acagcgaaga gttatcgggt atcagctccg ccgcgcggcg
     3001 cgcgatgtcg gtcttctcct cggccatcag gatcttgcgc gcgccgtaac cgaggttttc
     3061 gacgttatcc ttgacgatcg ccccgccgtg tacgcgctgc accagtttca gctcgtagag
     3121 ctggttcagg tcgcggcgaa tggtctgcgg cgttacctgc aggtgtgcgg acaactggtc
     3181 gacctggact gcgccgtgtt cgcgtatgta gtccatgatc aggtgctgcc ggctgctgat
     3241 ttcgacattc attgctgaaa gggattcgct aacgcccgga gtggggcgag tgccgggatt
     3301 tcagcaatga aaccccggca accgccgcaa tgaccgggtc agggtcgagc gcttactgct
     3361 tccagcgctt gaccagttct tcgtagttga cggtctgccc tttcggcttt tcgttgacct
     3421 tggccttcgg tgaaccgggc tggttcagcc agtaggaggc ttccttttcc tcgttgaggc
     3481 gcggtccgca gccgccatag gtattggcct tttcgtcggc cgcctgcatt cgcgacatga
     3541 ccaggtccat ttcgctggcg agacggtcca tggcctgctg cggtgtgaac gcgccggagt
     3601 tgacgtcacc gatctgctgc caccagatct gtgccagctt cgggtagtcg ggcacgttga
     3661 tgccggtcgg cgaccagagg acgcgatccg gcgagcggta gaattccacc aggccgccga
     3721 ggttcggcgc gcgatcggtg aacgactggt ggcggatggt gctgtcgcga atgaaggtca
     3781 ggccaacgtg ggatttcttg acgtcgacgg tcttcgaggt gacgaactgc gcatacagcc
     3841 aggcggcctt gcggcgatcg accggggtcg acttgaacag cgtccacgaa ccggcgtcct
     3901 gatagccgag cttctggcct tcttcccagt agggtccgtg cggcgacggt gccattctcc
     3961 acagcggttt gccgttatcg tcgacggtat tgttgccgtc gctcttcggc gccaccatcg
     4021 acgcggtgaa agcggtatac cagaaaatct gctgggcgac gttaccctgc gacagtgccg
     4081 gcagcgactg gtagaagtcg tagctggccg cgcccggagg tgcatacttg cgcagccact
     4141 cgtcccactt gcggatcgcg tagaccgagg ccgggccgtt ggctgcgccg ccgcgggtta
     4201 ccgaggcgcc gaccgggttg cagctgccgg actccatgcg gataccccat tcgtcgaccg
     4261 gtacgccgtt cggcaggccc ttgctgccag cgccagccat cgacagccag gcatcggtca
     4321 tccgccagcc taggtccggg gcgcgcttgc cgtagtccat gtggccgtag acgcgcacgc
     4381 cgtcgatttc cttgacatcc tcggaaaaga attcggcgat gtcttcgtag gccgaccagt
     4441 tgaccggcac gccgagatcg tagccgtact tggccttgaa gcgctgctgc aggtcggcgc
     4501 ggtcgaacca gtccttgcgg aaccagtaga gattggcgaa ctgctggtca ggcagctggt
     4561 agagcttgcc gtcgggaccg gtggtaaacg acttgccgat gaaatcgtcg acgtcgagca
     4621 tcgggttggt gactgccttg ccctcgccgg ccatccagtc ggacaggttg atcgccagct
     4681 gcaatcgcga atgggtgccg atcaggtcgg agtcattgat gtaggcgtcg tagagattac
     4741 gcttggtctg catctgcgtt tgtaccgcct gcacgacctc gccctcgccg agcagctggt
     4801 ggttgacctt gatgccggta atttcctcga acgccttggt cagggttttc gattcgtact
     4861 cgtgggtcgg aatggtttcc gacagcacgt tgatttccat gccggcgaag ggcttggcag
     4921 cgtctatgaa ccactgcatt tccttcagct ggtccgcgcg ggacagggaa gagggctgga
     4981 attcgtcgtc tacccatctt tcggcctcgg ccatgccggc gaaaacgggt tgtgtcgaca
     5041 gcgcggcaag ggtggctgcc gcagcaatct gcttaaacat gagatgtcct ccgttaattt
     5101 ttatgtcggg gtgccagtga gcacgataaa tctacgcggc catattcgca atgtcaatgc
     5161 atcgttttcg tttttttcgt attacatgtt cgaaatcttc gtttattttc gttttcaaat
     5221 gcaactgaag gcgtttttga tgccttcggc ccctgttcag cattgccccg agtctttatg
     5281 atcgcgctat ggcggaaacc agacagttgc tgatcgtcgc ccacaatccc tcgccgaata
     5341 cgcaaaagct ggtcgcggcg acgctgcgcg gcgcgcgtca cccggatatc gagggcgtcg
     5401 aggctcggca cgtgccgccg ctcgaggcgg tggtcgatga cgtattgggc gccgacgcga
     5461 tcattctcgg caccaccgag aacttcggtt acatgagcgg cgcactgaag gactttttcg
     5521 accgcatcta ttatccctgc ctggagcaca ccgaggcgat gcccttcgcc acctggattc
     5581 gcgccggcct cgacggtacc ggcacgaagg tcgcgatcgg caagatcacc agcgggctca
     5641 aatggaaggc cgtgcagcaa ccactgatcc tgcacggtga gtaccgcacc gagttcgaag
     5701 accagtgcga ggaactcggc atgctgatgg ccgccagcct cgagtccggc atcatctaat
     5761 tcgggtcaga tccaaattaa gacctcccat taattcgggt cagatccaaa ttaagacccg
     5821 ccccttaatt cgggtcagat ccaaattaag acccgcccct taattcgggt cagatccaaa
     5881 ttaagaccca ccctttaatt cgggtcagat ccgaattaat ggacgaggtg taatttggat
     5941 ctgacccaaa ttaatgggat gcagcgggtt gcgggaatcg gctatgattt gcggactatg
     6001 cgggacccgg gcagccgggg attttcgaca atcacacagg tacccagcgc atgaataaat
     6061 ttcagggcaa gaaggcgatc gtgctcggcg gcacctcggg gatcgggctg gcggcgaccg
     6121 agatgttgcg cgatggtggc gccgaggtca tcgccggcgg ccgccgcgaa tgcgagatcg
     6181 acggcgtcga gaccgcgcgg atcgacgtgc tcgatcgcga cggcctgcag gcgctgttcg
     6241 aaaaacacca gggcttcgat tacctggtca atgcggctac cggcgggccg cgcgccatcg
     6301 ggcccttcct ggaaatggac ctcgacggtt tccagggctc gttcgccaag ctgtggggtt
     6361 acgtcaacag cgtgcgccta ggcgtgccgc tgatgtcgaa gcagggcgcg gtggtgctgt
     6421 tcagcggcta cccggcgcgc aagtaccgcc ccggtacgct cgccatcggc agcgtcggcg
     6481 gttcggtgga agccttcatt cagctggtcg ccaacgagat cgcgccggtg cgcatcaacg
     6541 gtgtctgccc ggggctgatc gatacaccga tgtcgccgct cgagggcgag gcgcggcgca
     6601 actactatgc cgacggcacg cgcaaccacc tgattccgcg tgccggcacg ccggccgagt
     6661 gcgctggcgc ggccatattc ctgctcgaga acgagttcgt gaccggcacc attgtcgatg
     6721 tcgacggcgg cgccatctgt acatgagctg cgaaaaaatc tgccgcagcc tggccggtga
     6781 tacgccgggc cgcagcaccg agctgaatta cttccgtatc ggccccgcgc aggccgacag
     6841 gaaggtctac ctgcaggcgg cgctgcactg tgacgaacag cccggcatcc tgatcctgca
     6901 ccatttattg caactgttgc gcgaggccga cgagcacgac cagctggccg cgcagttcgt
     6961 gctgtttccg atggtcaatc cgctcggcat gagcgacatc cagttc
//
LOCUS       DATCZV010000460        13488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_779958, whole genome shotgun sequence.
ACCESSION   DATCZV010000460 DATCZV010000000
VERSION     DATCZV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779958"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VIW27_10320"
     CDS             <1..585
                     /locus_tag="VIW27_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740111.1"
                     /translation="IGSVKAWFRIHMILGVAGPLMIIFHSGFKLGSFNSSVALFCMLL
                     VAGSGLIGRYLYQRIHHGLYGSKVRFEELYHDDELTSLLLSEAGGIDFELVDELEDIK
                     EKLTPRHTGINRSIGFYRKMRSRIDRLSRRVRRSNLGVAEKKAARRRLHDLRQICVLG
                     INEILFSFWHLLHLPLFVVMVLSGLIHVAVVHLY"
     gene            585..2189
                     /locus_tag="VIW27_10325"
     CDS             585..2189
                     /locus_tag="VIW27_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5740112.1"
                     /translation="MRGLRIALLLLGAMLCGSAAAAEIEQFFMPGKLISGHQEYEAEC
                     TRCHVRLRDTTQKKLCLDCHDLVAEDLRRKRGFHGRDRNASNLDCKACHSDHKGRDAR
                     VVWLDRDNFHHGDTDFRLAGRHQLVECTACHQPGDKFREAPQDCYSCHSEDDAHRGEL
                     GKQCGDCHAPSGWARSEFDHDRADFKLRDAHRRVSCDACHLDTAYDRTPSRCVDCHAV
                     KDVHDNRFGTQCQDCHTEKKWADTIFDHGRDTDYRLEDAHRKVSCNACHGRDYLASKK
                     ANTVRSCYSCHRADDAHNESNGKKCQDCHSPRGWTYAEFDHDANTDFALNGAHTDLVC
                     AACHAVGAESREIDSDCYSCHKLDDSHAQEQGTDCARCHNEVGWQMNVRFDHELTEFP
                     LLGQHAATGCETCHLSSVFREAGEQCADCHAGDDPHRQALGRDCARCHNPNAWLIWRF
                     DHDQTGFELRDVHAEAHCHTCHREPLRASSEQDWRCVDCHWRDDVHEGNFGGACDNCH
                     DRQRFDNIDPQALRSLGFGATREPRR"
     gene            2186..2971
                     /locus_tag="VIW27_10330"
     CDS             2186..2971
                     /locus_tag="VIW27_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5740113.1"
                     /translation="MSTIPVGARRKLPPRRRPARFALLLAALAGSLLAADSPAQEARI
                     EFDHDATIFPLEFKHALASCESCHQQGVFAGTPRQCRDCHANTGRIKASAPPSGHIRF
                     LGDCDSCHRPDSWFTVLRVDHAAVAGSCANCHDGFVASGKNLGHVQSTNSCDDCHMTL
                     TWTGAVFNHVDIAGNCIACHDGFTASGKHTAHILSAASCGDCHTTFGWQPVTRVDHGA
                     VIGSCFSCHNGVVAGGKHALHEPSSDECQLCHSPLGWTPAISP"
     gene            3013..5100
                     /locus_tag="VIW27_10335"
     CDS             3013..5100
                     /locus_tag="VIW27_10335"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY5740114.1"
                     /translation="MQSRYRGYREPPPAACPRNLAAAALRLGLVLTCLFAAGGACAAN
                     YEFLAFENFYGDAAEITVIHPFAIVLRSSKQPFGELSRGTRGALRNQFVYQTRVKVGG
                     QTFYRLAAGNFADAAAARSALQKLRPWFKDAWVYRREEPEGQTLRARLQTTTASATGS
                     RELTNNPPRADDDLALQAKQAFIDQDYARVLRITEQILLRGNSDDVRMALELAGVVHE
                     RRGNYAEAVRLYESLLETDPPPAVANRIGSRLQGILTMREQPREPLPTGQQQAAREWQ
                     ARGVFQQYYRNDTLDWSDRDSDTINELLATDIDLHLRGSTDSNTWSLRLDGGLVRDLA
                     ERQTDPRVARAGIGYANDDFSITAGRQDNSATGVFGRFDGFTLTDFSRSGYQLTYVAG
                     YQVQSSFDDLETDNPLLGAKLDLHPFDWLDTSLYLVEREISELTDRRAVGGEFQLRNR
                     RGYLYGIVDYDLFYERLNNIQLISSYRYGDRLTLNLNLGRASSPPLATINALQGQSIE
                     SIDELRELFDDEQIYQLAEDRTSNRDSLYFGTIYRIDDNRQLYVDISVLDSEPTPASG
                     GVTALPGFEDILLAADYSVNGLFGAGNHTVIGLRLADTTSSKTESLRLRSHFRGAGGF
                     AYTPGIRVERRHDTAEGADRYLLNPVLKLSYRPRDRLSFEADLAIEYSDFDLPDHDPQ
                     TLKSLYLGYAYVF"
     gene            5245..8697
                     /locus_tag="VIW27_10340"
     CDS             5245..8697
                     /locus_tag="VIW27_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740115.1"
                     /translation="MRTVKTLIETAAGYLAAAIEPAGTRQRREPSVGCGGTGLRIGLP
                     NPAAAALPMLLILTLSITACGGGGDGGASAVAVPDPTPDPDPEISLVARTGPIVAVRV
                     GETAMLSDNNSYTTSQQLLRFQWSFSHRPEASSAVLQGETSRNPSFVADVKGAYLVQL
                     TVSAGGQVSTRALQQVIASDPSERLTGPFTHQGLSSSCVICHSDTVDLLPGGGMIGGK
                     SATHIAASSNCEGCHTPLGFTAVPYVDHREVFGVCSDCHDGTIAVGKSEFHPATEAEC
                     DSCHNTDHFLARLPDGSFDHTGITRACSGCHNGTVARGKTPAPPHPETASECGYCHTV
                     DSFTGAYPDHSGPDVVGKRCDSCHGNGAASSAIGEPPGHPVMNPDCAACHTVSGFSLG
                     GVFHHRIEPLQQPCESCHNDDANSNFGGLALGKGSAVPAHPATTSSCDSCHSTDSFSP
                     AFGIDHTDPAILAARCDSCHGVSASGKSADHLPTLEDCGACHTPGSFATGSFSHDPGY
                     LAPPAQCADCHDNVISVGKLPSHFPTPLGAAQDCADCHDTVAFAGTPFDHAGIDPNNC
                     AQCHNGDYGTTANSLYGKPPTHLPTSQDCSVCHSFSAPFSPALNFAHLGITDNCESCH
                     NGNPAYVAVGALGKKPDHIPAEEQCSNCHVDKTAGGFASSTFLATLHPGISNGCEGCH
                     VSRFFATRPDLTKGASHLPTAQDCDLCHTVAGFGPPTRFAHVDITGDCASCHSGNPDF
                     ELLGARGKTPSPPHPATTADCADCHNTVNFADAFVDHSAPAILAVRCDSCHNGIDATG
                     KNAAPGHVTTSEDCSVCHLAGGAFTPAVFDHSGIVDNCASCHNGVDATGTTAKTNPPH
                     IPITQDCSVCHTPTLFANARFEHQGIVDNCAGCHDGFTATGKGTFHVPTSDDCGSCHV
                     TTGFLPARFDHVGIVDNCAACHAAGFATPKSIDHVATTQDCGVCHNTGAFVPATFDHT
                     GIVDNCASCHDGNTATGMADAVPPHLPTSLDCHFCHVTATFVGGGWVHDASTANNCDS
                     CHAAGGGATPKSLGHLNTTEQCDQCHTTTSWAPSSFSHDPGGNYPGDHRRNPGCNACH
                     GTTISSTIPWPFTRYAPFCAACHANSFESEGAHNGGRNGTVEQNKDCSGGGRGCHRVS
                     DSEF"
     gene            9101..10075
                     /locus_tag="VIW27_10345"
     CDS             9101..10075
                     /locus_tag="VIW27_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5740116.1"
                     /translation="MKPEKILPASRAALLCAALLPLSSFAADVKVTPLGSQEGEFCQL
                     DRAMIFEDPDGTRILYEAGRTVAGADDPRLGKIDAVLVSHMHGDHVGDRHILTPGDGA
                     CEDSAFPEGALPATNTVAIAHAKGAKIVTGSEMPKFFAAKLESVGGNPQDSQLVRFGA
                     SRNVGGVTITTVPATHSNGIAGAMIGGDLGSLLNASGLTAYAGPPTGYVLTFSNGLVA
                     YLSGDTGITAEQKTVVRDHYEAKLVVMNIGNTFTTGPQEAAWVVNELIKPASVIPSHA
                     NQPSTRDGKVIAGTRVEQFSELAKARVHIPLSGRTMSFNAKGKCTGGC"
     gene            complement(10173..10553)
                     /gene="rplQ"
                     /locus_tag="VIW27_10350"
     CDS             complement(10173..10553)
                     /gene="rplQ"
                     /locus_tag="VIW27_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013797818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY5740117.1"
                     /translation="MRHRNSGRQLSRNSSHRKAMFRNMTASLFDNEVIRTTLPKAKEL
                     RRVAEPLITLAKEDSVANRRVAFSRIRDKAAVGKLFSDLGPRYKARPGGYLRILKCGY
                     RAGDKAPMAYVELVDRPDVELDDE"
     gene            complement(10567..11562)
                     /gene="rpoA"
                     /locus_tag="VIW27_10355"
     CDS             complement(10567..11562)
                     /gene="rpoA"
                     /locus_tag="VIW27_10355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008959151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY5740118.1"
                     /translation="MSKMYSDLLKPKHVKVEEQHTYHARVSIEPLERGFGHTLGNALR
                     RILLSSVRGCAIVDCRIEGVLHEYTTIDGVQEDVIDILLNLKGVGIIMHSKDEATLTI
                     SKKGPGLVTAGDIQLDHDIEIVDPDYLIATLTKAIDFEMSLHLMKGRGYQPANTRQIS
                     EEDTALGHLQLDASFSPVRKIAYSVEAARVEQRTDLDKLIIDIETNGTIDPEEAIRIA
                     GSILKDQLSVFTNLEPQDEDSETEPESHIDPILLRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTEVELLKTPNLGKKSLTEIKDVLASYSLSLGMRLENWPPASIDNDKLAS"
     gene            complement(11577..12203)
                     /gene="rpsD"
                     /locus_tag="VIW27_10360"
     CDS             complement(11577..12203)
                     /gene="rpsD"
                     /locus_tag="VIW27_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5740119.1"
                     /translation="MAKYIGPKCKLMRREGADLSLKSSRRAVDTKCKIDNPPGMHGAG
                     TRKPRQSDYGLQLREKQKLRRIYGVLERQFRNYYKEASRLKGSTGENLLQLLETRLDN
                     VVFRMGFGSTRAEARQLVNHKAILVNGKIVNIPSYKVRPDDVVSVREKSRKQARIAEA
                     MALAEQIGLPGWVDVNSSRFEGVFKALPGRSDLPPDINEQLVVELYSK"
     gene            complement(12216..12602)
                     /gene="rpsK"
                     /locus_tag="VIW27_10365"
     CDS             complement(12216..12602)
                     /gene="rpsK"
                     /locus_tag="VIW27_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY5740120.1"
                     /translation="MVDKSRSKKKARRVVSDGVAHIHATFNNTIVTISDRQGNALTWA
                     TAGGSGFRGSRKSTPFAAQVAAERAGQAAQEMGMKNLEVMVKGPGPGRDSAVRALNAI
                     GFKISRIDDVTPIPHNGCRPPKKRRV"
     gene            complement(12626..12982)
                     /gene="rpsM"
                     /locus_tag="VIW27_10370"
     CDS             complement(12626..12982)
                     /gene="rpsM"
                     /locus_tag="VIW27_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY5740121.1"
                     /translation="MARIAGVNIPDSKHTVIALQSIFGIGPTRSAEICSATGIDPQTK
                     VRELSEEQLEQIRLRVDTYTTEGDLRREISMNIKRLMDLGTNRGIRHRRGLPVRGQRT
                     KTNARTRKGPRRPLKR"
     gene            complement(13069..13182)
                     /gene="rpmJ"
                     /locus_tag="VIW27_10375"
     CDS             complement(13069..13182)
                     /gene="rpmJ"
                     /locus_tag="VIW27_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY5740122.1"
                     /translation="MKVRASVKKMCKNCKVIRRNGSVRVICTDPRHKQRQG"
     gene            complement(13204..>13488)
                     /gene="secY"
                     /locus_tag="VIW27_10380"
     CDS             complement(13204..>13488)
                     /gene="secY"
                     /locus_tag="VIW27_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232158.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5740123.1"
                     /translation="GIRPGNQTANYIDGVMSRLTLVGAIYITAVCLLPEFLILFWNVP
                     FYFGGTSLLIIVVVVMDFMSQVQAHLMSHQYDGLMKKSNFKGYGGAGVVR"
BASE COUNT         2491 a         4548 c         4045 g         2404 t
ORIGIN      
        1 atcggctcgg tcaaggcctg gtttcgaatc cacatgatcc tcggcgtggc cggtccgctg
       61 atgattattt ttcactccgg cttcaaactc gggtcgttca acagcagcgt cgcgctgttc
      121 tgcatgctgc tggtggccgg cagcggcctg atcggccgtt acctgtacca gcgaatccat
      181 cacggtctgt acggtagcaa ggtccgcttc gaggagcttt accacgacga cgagctcacc
      241 agcctgctgt tgagcgaggc cggcggcatc gatttcgaac tcgtcgacga actcgaggac
      301 atcaaggaaa agctgactcc ccggcacacc ggcatcaacc gctcgatcgg tttctaccgc
      361 aaaatgcgct cgcgcatcga ccgcctgtcc cgaagggtac gccgctcgaa tctcggcgtg
      421 gccgagaaaa aagcagcccg gcggcgcttg cacgacctgc ggcagatttg cgtgctgggg
      481 atcaacgaaa tcctgttctc tttctggcac ctgctgcatc tgccgctgtt cgtggtgatg
      541 gtgttgtcgg gcctgataca tgtcgccgta gttcatttgt actgatgcgc gggctgcgga
      601 ttgcgctgct gctgctcggc gcaatgcttt gcggctcggc ggccgccgcc gaaatcgagc
      661 agttcttcat gcccggcaaa ctgatttccg gccaccagga gtacgaagcg gaatgcaccc
      721 gctgccacgt gcggctgcgc gatacgacgc agaagaagct gtgcctggac tgccacgacc
      781 tggtcgccga ggacctgcgc cgcaagcgcg gcttccacgg ccgggacaga aatgcatcaa
      841 acctcgactg caaggcttgt cacagcgatc acaagggccg ggacgcgcgc gtggtatggc
      901 tcgaccgcga taattttcat cacggcgaca ccgatttccg cctcgccggg cgccaccagc
      961 tggtcgaatg cacggcctgt caccagccgg gcgacaagtt ccgcgaggcc ccgcaggact
     1021 gctattcctg tcattccgag gacgacgctc accgcggcga actcggcaag caatgcggcg
     1081 attgccacgc gccgagcggc tgggcgcgct ccgagttcga ccacgaccgc gccgacttca
     1141 agttgcgcga cgcccaccgg cgcgtcagtt gcgacgcctg tcatctcgat accgcctacg
     1201 acaggacccc gagccgctgt gtcgactgcc atgcggtcaa ggacgtgcac gacaaccgct
     1261 tcggcaccca gtgccaggac tgccacacgg agaaaaaatg ggccgatacc attttcgatc
     1321 acggccgcga taccgattac cggctcgagg acgcccatcg caaggtcagc tgcaatgcct
     1381 gtcatgggcg cgattacctg gcctcgaaaa aggccaacac ggtccgcagc tgctacagct
     1441 gtcatcgcgc cgacgacgcg cacaacgagt cgaacggcaa aaaatgccag gattgccatt
     1501 cgccgcgggg ctggacctac gccgaattcg atcacgacgc gaacaccgac ttcgcgctca
     1561 acggcgccca caccgacctg gtctgcgcgg cctgccacgc ggtcggcgct gaatccaggg
     1621 aaatcgacag cgattgctac agctgccaca agctcgacga cagtcacgcg caggaacagg
     1681 gcaccgattg cgcgcgttgc cataacgagg tcggctggca aatgaacgtg cgcttcgacc
     1741 acgaactgac cgaatttccg ctgctcggcc agcacgcggc cacgggctgc gaaacctgcc
     1801 acctgagctc ggtgtttcgc gaggccggcg agcaatgcgc cgactgccat gccggcgacg
     1861 acccgcaccg ccaggcactg gggcgggatt gtgcccgctg ccacaacccc aacgcctggc
     1921 tgatctggcg attcgaccac gaccaaaccg gcttcgagct gcgcgacgtc cacgccgaag
     1981 cgcattgcca tacctgtcac cgggagccgt tgcgcgccag cagcgagcag gactggcgct
     2041 gcgtcgattg tcactggcgc gatgacgtgc acgagggcaa tttcggcggc gcctgcgaca
     2101 attgccacga tcggcagcgt ttcgacaaca tcgatccgca agcactgcgc agcctgggct
     2161 tcggtgccac ccgggagccg cggcgatgag cacgattcct gtcggcgctc gccgcaaact
     2221 gccaccacgc cgtcgcccgg cccgcttcgc gctgctgctg gcggcgcttg ccggcagtct
     2281 gctcgcggcc gacagcccgg cgcaggaagc gcgcatcgag ttcgatcacg atgccacgat
     2341 ctttccactc gagttcaagc acgccctcgc gagctgcgaa tcctgtcacc agcagggggt
     2401 tttcgccggc accccgcggc aatgccgcga ctgccatgcc aacaccggcc gcatcaaggc
     2461 aagcgcgccg ccaagcggcc acattcgctt tctcggcgac tgcgattcct gccaccggcc
     2521 cgattcctgg ttcaccgtgc tcagggtcga tcacgcggcg gtggccggca gctgtgcaaa
     2581 ctgtcacgac ggcttcgtcg ccagcggcaa aaatctcggc cacgtgcagt ccaccaacag
     2641 ctgcgacgat tgccacatga cgctcacctg gacgggcgcg gtcttcaacc atgtcgacat
     2701 cgccggcaat tgcatcgcct gtcacgacgg cttcaccgcc agcggcaagc acacggctca
     2761 catactgtcg gcagccagct gcggggattg ccacaccacc ttcggctggc agccggtgac
     2821 gcgggtcgat cacggcgcgg tgatcggcag ctgcttcagt tgtcacaacg gcgtcgtcgc
     2881 cggcggcaag cacgcgctgc acgaaccgag cagcgacgaa tgccagctgt gccacagccc
     2941 gctcggctgg actccggcga tcagccccta ggcggcccga cgatgactgc ccgccggcaa
     3001 tttttcggat tcatgcaatc gcggtatcga gggtatcgcg agccaccgcc tgctgcctgc
     3061 ccgcgtaacc tggcggcagc cgcgctgcgg ctgggcctgg tattgacctg cctgttcgcg
     3121 gccggcgggg cctgcgccgc caattacgag tttctggcgt tcgaaaactt ctatggcgac
     3181 gccgccgaga tcacggtcat ccatccgttc gcgatcgtcc tcaggtcgag caagcagccc
     3241 ttcggcgagc tgtcgcgggg cactcgcggc gcgctgcgca atcaattcgt ctaccagacc
     3301 cgtgtcaagg tcgggggaca gacgttttac cgcctggcgg cgggaaattt cgccgacgcg
     3361 gcggcggcca ggtcggccct gcaaaagctc aggccctggt tcaaggatgc ctgggtttac
     3421 cgccgcgagg agccggaggg gcagacgctg cgcgcgcgct tgcagaccac gaccgccagc
     3481 gccactggca gccgcgagct gacgaataac ccgccgcgcg ccgacgacga tctggcactg
     3541 caggcaaaac aggcgttcat cgaccaggat tacgcgcgcg tgctgcgcat taccgagcaa
     3601 atcctgttgc gcggcaacag cgacgacgtg cgcatggcgc tcgaactcgc cggggtcgtc
     3661 cacgaacgtc ggggaaacta tgccgaggcg gtcagattgt acgaatccct gctcgagacc
     3721 gacccgccgc cggcggtcgc caaccggatc ggcagccgcc tgcagggtat tctcacgatg
     3781 cgcgagcaac cccgggagcc gctgccgacg ggccagcaac aagccgcccg cgaatggcag
     3841 gcacgcggtg tctttcagca gtattaccgc aacgataccc tcgactggtc ggaccgcgac
     3901 agcgatacga tcaacgaact gctggccacc gatatcgacc tgcacctgcg cggcagcacg
     3961 gacagcaata cctggtcgct gcggctcgat ggcgggctgg tcagggatct cgccgagcga
     4021 cagaccgacc cgcgagtggc gcgcgccgga atcggctacg ccaacgacga tttcagtatt
     4081 accgccgggc gccaggacaa cagcgcgacc ggcgtcttcg gccgcttcga cggctttacg
     4141 ctgaccgatt tttcacgctc cggttaccag ctcacctatg tcgcgggtta ccaggtgcag
     4201 tcgtctttcg acgacctcga aaccgacaat cccctgctcg gcgcaaagct cgacctgcat
     4261 ccctttgact ggctggacac cagcctgtac ctggtggagc gcgaaatatc ggagctgacc
     4321 gaccgccgcg ccgtcggcgg cgagttccag ctgcgcaatc ggcgcggtta cctttacggc
     4381 attgtcgatt acgacctgtt ctacgagcgc ctcaacaaca tccagctgat ttccagttat
     4441 cgctacggcg atcgcctgac cctgaacctg aacctgggac gggcgagttc accgccgctg
     4501 gcgaccatca acgcgctgca gggccagtcg atcgagtcga tagacgaact gcgcgagctg
     4561 ttcgacgacg agcagattta ccagctggcc gaggatcgca ccagcaaccg cgattcgctg
     4621 tacttcggca ccatttaccg catcgacgac aaccgccagc tgtatgtcga tatatcggtg
     4681 ctcgacagcg agccgacccc ggcctcggga ggggtgaccg ccctgcccgg cttcgaggac
     4741 atactgctgg cggccgacta ctcggtcaac ggattgttcg gcgccggcaa tcacaccgtc
     4801 atcggcctgc gcctcgcgga taccaccagc agcaagaccg aatcgctgcg cctgcgcagc
     4861 cattttcgag gagccggcgg cttcgcttat accccgggga ttcgcgtcga acgccgccac
     4921 gacaccgccg agggtgccga ccgctacctg ttgaatccgg tgctgaagct cagctaccgg
     4981 ccgcgcgacc gcctcagttt cgaagccgac ctggcgatcg agtattcgga tttcgatttg
     5041 cccgatcacg acccgcagac gctcaagtcg ctctacctcg ggtatgccta tgtgttctag
     5101 atcggcgaat gctttgccgc ttgtggcggc cggcgcgctg ccggcacgga tggcggcatt
     5161 gttatcggcc ggcggcggga ttgtcgccgg ccagggatat gaatcagtca ggaccatgat
     5221 caggttcggg aagggaaatg taccatgcga acagttaaaa cattgatcga aaccgcggcg
     5281 ggttacctgg ccgccgcaat cgaacccgca ggcacgcgcc agcgccgtga gccgtccgtc
     5341 ggctgcggcg ggaccggttt gcgaatcggc ttgccgaatc ctgcggcggc ggcgctgccg
     5401 atgctgttga tactgaccct gtcgatcacc gcctgcggcg gtggcggcga tggcggggcc
     5461 agcgcggtgg cagtacccga cccgaccccg gaccccgatc cggagatcag cctggtcgcc
     5521 aggaccgggc ccatcgtcgc ggtcagggtc ggtgaaacgg cgatgctgtc cgacaacaat
     5581 tcctatacca cgtcccagca actgctgcgc tttcagtggt cgttttccca ccggcccgag
     5641 gccagcagcg cggtgttaca gggcgagacc tcgcgcaacc cgagcttcgt cgccgacgtc
     5701 aagggcgcct acctggttca gctgacggtc agcgccggcg gccaggtcag cacgcgcgcg
     5761 ctgcagcagg taatcgccag cgatccgtcc gagcggctca ccggtccttt cactcaccag
     5821 ggattgtcat cgagctgcgt gatttgccat agcgacaccg tcgacctgct gccggggggc
     5881 ggtatgattg gcggcaagtc ggcgacccat atcgccgcca gcagcaactg cgaaggctgc
     5941 catacgccgc tcggattcac ggccgtgccg tacgtcgatc accgcgaagt tttcggtgtc
     6001 tgcagcgact gccacgacgg caccatcgcc gtcggcaaat ccgagtttca tccagcgacc
     6061 gaggccgagt gcgacagctg ccacaacacc gatcacttcc tcgcccggct gcccgacggc
     6121 agcttcgatc acaccggcat cacccgcgcc tgcagcggtt gtcacaacgg caccgtggcc
     6181 aggggcaaga ccccggcgcc accgcacccc gaaaccgctt ccgaatgtgg ttactgtcac
     6241 acggtcgaca gctttaccgg cgcctatccc gaccatagcg gtccggacgt ggtcggcaag
     6301 cgctgcgact cctgccacgg caacggcgcc gccagcagcg cgatcggcga accgccgggg
     6361 cacccggtga tgaatcccga ctgcgcggcc tgtcacacgg tcagcggctt cagcctcggc
     6421 ggcgtctttc atcatcgcat cgagccgctg cagcagccct gcgagagctg ccacaacgac
     6481 gatgccaata gcaatttcgg cggtctcgcg ctcggcaagg gcagcgcggt gcccgcccac
     6541 ccggcgacca cctcttcctg cgacagctgc cacagcaccg acagtttcag cccggcgttc
     6601 ggtatcgacc ataccgaccc ggcgatactg gcggcacgct gcgactcctg ccacggcgtc
     6661 tcggcgagcg gaaaatccgc cgatcatctg ccgacactcg aggattgcgg cgcttgccat
     6721 acccccggca gctttgccac cggcagtttc agccacgacc cgggctacct ggcgccgccc
     6781 gcacaatgcg ccgattgcca cgataacgtg atcagcgtcg gcaagttgcc gagccacttc
     6841 ccgacgccac tcggcgccgc ccaggattgc gccgactgcc acgacaccgt cgcatttgcc
     6901 ggcacgccct tcgaccatgc gggaatcgat ccgaataact gcgcgcaatg ccacaacggc
     6961 gactacggca ccacggccaa ctcgctgtac ggcaaaccgc ccacgcattt gccgaccagc
     7021 caggactgca gtgtctgtca cagtttttcg gcaccgttct cgccggcgct caacttcgcg
     7081 catctcggca tcaccgacaa ttgcgaatcc tgccacaacg gcaatcccgc ttacgtggcg
     7141 gtcggcgcgc tcggcaagaa gcccgatcat atcccggcgg aagagcagtg cagtaattgc
     7201 catgtcgaca aaaccgccgg cgggtttgcg tcatccacgt ttctcgccac gctgcacccc
     7261 ggtatcagca acggctgcga gggttgccac gtcagccgct tcttcgccac gcggcccgac
     7321 ctgaccaagg gcgccagcca cctgccgacc gcccaggatt gcgacctgtg ccacacggtc
     7381 gccggtttcg ggccgccgac ccgcttcgcc catgtcgata tcaccggcga ctgcgcctcc
     7441 tgccatagcg gtaatcccga tttcgaattg ctgggggccc gcggcaagac gccgtcgccg
     7501 ccccacccgg cgaccaccgc cgactgcgcg gattgccata acacggtcaa tttcgccgat
     7561 gccttcgtcg accattccgc tccagccatc ctcgccgtgc gctgcgattc ctgtcataac
     7621 ggcatcgacg cgaccggcaa gaacgcggct cccggccacg tgacgaccag cgaggactgc
     7681 agcgtctgcc acctggcggg cggtgcgttc acgccggcgg tgttcgacca cagcggcatc
     7741 gtcgataact gcgcgtcctg tcacaacggt gtcgatgcca ccggcaccac cgccaagacc
     7801 aatccgccgc acatcccgat cacccaggat tgctcggtct gccatacgcc gacactgttc
     7861 gccaacgccc gcttcgagca ccagggtatc gtcgacaact gcgccggctg ccacgacggc
     7921 ttcaccgcga ccggcaaggg cactttccac gtgcccacca gcgacgattg cggcagttgc
     7981 cacgtgacca ccggattcct gccggccaga ttcgaccatg tgggcatcgt cgataactgc
     8041 gctgcgtgcc acgctgccgg cttcgcgacg ccgaagagca tcgatcacgt cgcgactacc
     8101 caggattgcg gtgtctgtca caataccggc gccttcgtgc cggcgacctt cgaccatacc
     8161 ggcatcgtcg acaactgcgc ctcgtgtcac gacggcaata ccgcgaccgg catggccgac
     8221 gcggttccgc cgcatctgcc gaccagcctc gattgccatt tctgccacgt caccgccacc
     8281 ttcgtcggcg gcggctgggt gcacgatgcc agcaccgcca acaactgcga tagctgccac
     8341 gccgcgggcg gtggcgcgac tcccaagtcg ctcggacacc tgaacacaac cgagcaatgc
     8401 gaccagtgcc ataccaccac cagctgggcg ccgagcagct tcagtcacga ccccggcggc
     8461 aattatcccg gcgatcaccg caggaacccg ggttgcaacg cctgccacgg caccaccatc
     8521 agcagcacga ttccgtggcc cttcactcgc tacgcgccct tctgtgccgc ctgccacgcc
     8581 aacagcttcg aatccgaggg tgcgcacaac ggcggcagaa acggcaccgt cgagcaaaac
     8641 aaggattgca gcggcggcgg ccgcggctgc caccgggtca gtgacagtga attctagtcg
     8701 gcgggccgcg cactccgccg ctgactccga tccgggcgac gctggtcccg cggcggcggc
     8761 agggattatt cgagcggctg ccggcgccgt tcccaccgtg ctccgtgggg ctgggcgggc
     8821 tgcgcatcgt gccgagcgag tgactgccgc cagccggccc tgcactccga tagcggccgg
     8881 cccgggaacg cggcgccatg cagcggcgaa ctccggtgct cgatccgccg cattcggcct
     8941 gccgcacaga aacgcagact cgctctcgaa gttgggttaa catattccct cgaaacggac
     9001 agcggtcgcc gtcgtccgtc gccggcggcg cgatgcaatc cgccgcggat cctgcaacaa
     9061 accctaacga tagcaacaga caaaaccaag gagttgcctg atgaaaccag aaaaaattct
     9121 ccccgcttcc cgggcggcgc tactgtgcgc ggcgctgttg ccgctgtcct cgttcgctgc
     9181 cgatgtcaag gtgacaccgc tgggcagcca ggagggtgag ttctgccagc tcgaccgggc
     9241 gatgatattc gaggacccgg acggcactcg aattctctat gaggccgggc gcacggtcgc
     9301 cggagccgac gatccgcggc tcggcaagat cgacgccgtc ctcgtcagcc acatgcacgg
     9361 cgatcacgtc ggcgaccgac atatcctgac gccgggcgac ggcgcctgcg aggacagcgc
     9421 atttccggaa ggcgcgttac cggccaccaa taccgtggcg atcgcccacg ccaagggtgc
     9481 caagatcgtc accggcagcg agatgccaaa atttttcgcc gccaaactgg agtcagtcgg
     9541 gggcaatccg caggattcgc aactggtgcg tttcggcgct tcgcgcaacg tcggcggggt
     9601 gacgatcacc acggtaccgg cgacgcactc caacggcatc gccggcgcga tgatcggcgg
     9661 cgatctcggc agcctgctga atgcctccgg gctgaccgcc tacgccggcc cgccgaccgg
     9721 ctacgtgctg accttttcca acggcctggt ggcctacctg tcgggcgata ccggcattac
     9781 cgccgagcag aaaaccgtgg tgcgcgatca ttacgaggcg aagctggtgg tgatgaatat
     9841 cggcaatacc tttaccaccg gaccgcagga agcggcctgg gtggtcaacg agctgatcaa
     9901 gcccgcatcg gtcattccgt cgcacgccaa ccagccttcc accagggacg gcaaggtcat
     9961 cgccggaacg cgtgtcgagc agttttccga gctcgccaag gccagggtcc acattccgct
    10021 gagcggacgc acgatgtcct tcaatgccaa gggcaaatgc accggcggct gctgacccga
    10081 accgccaggc cggcccgcgg gtcggcgagc cagcggcaat aaaacaagcc ggcttgacgc
    10141 cggctttttt ttattcactt gccgctaatg cgctattcgt cgtcgagctc gacatcggga
    10201 cgatcgacca gctcgacgta ggccatcggc gccttgtcac cggcccggta tccgcacttg
    10261 aggatgcgca ggtagccgcc gggacgcgcc ttgtagcgcg gcccgaggtc gctgaacagc
    10321 ttgccgaccg cggccttgtc gcgaatccgg ctgaaagcga cgcgccggtt ggcgacgcta
    10381 tcttccttcg ccagcgtgat caacggctcg gccacgcggc gcagttcctt ggccttgggc
    10441 agggtggtgc gaatcacttc gttatcgaac agcgaagccg tcatgttgcg aaacatcgcc
    10501 ttgcgatggg aactgttgcg gctgagctgc cgcccgctat tacgatggcg catcgtctaa
    10561 tcctcgttaa ctcgctagct tgtcgttatc gatgctggcc ggcggccagt tttccagccg
    10621 catgcccagc gacaggctgt aggaggccag cacgtccttg atctcggtca gcgacttctt
    10681 gcccaggttc ggggtcttca gcaattcgac ctcggtacgc tggatcaggt cgccgatata
    10741 gtaaatgttt tccgccttca ggcagttggc cgaacgcacg gtcagctcga gatcgtcgac
    10801 cgggcgcagc aggatcggat cgatgtgcga ttccggctcg gtctcggaat cctcgtcctg
    10861 cggctcgagg ttggtgaaca ccgacagctg atccttgagg atgctgccgg cgatgcggat
    10921 cgcttcctcg ggatcgatgg tgccgttggt ctcgatatcg atgatcagct tatcgaggtc
    10981 ggtgcgctgc tcgacgcgcg cggcttccac actgtaggcg atcttgcgca ccggcgaaaa
    11041 cgatgcatcg agttgcaggt gcccgagcgc ggtgtcttcc tcggaaatct gccgcgtgtt
    11101 ggccggctgg taaccgcggc ctttcatcaa gtgcagcgac atttcgaaat cgattgcttt
    11161 ggtcagcgtg gcgatcaggt aatccggatc gacgatttcg atatcgtggt cgagctgaat
    11221 gtctcccgcg gtgaccaggc cggggccctt cttggagatg gtcagcgtcg cctcatcctt
    11281 gctgtgcatg atgatgccga cacccttcag gttcagcagg atatcgatga cgtcttcctg
    11341 gacgccgtcg atggtggtgt attcgtgcaa tacaccctcg atgcggcaat cgacgatcgc
    11401 gcagccgcgc accgacgaca gcaggatgcg ccgcagcgca ttgccgaggg tatgaccgaa
    11461 accgcgctcc agcggctcga tcgaaacccg cgcatgatag gtatgctgtt cctcgacctt
    11521 gacgtgcttc ggcttcagta aatcagagta cattttagac ataaaatatc cgctagttat
    11581 ttcgagtaaa gttcgacaac caactgctcg ttgatatccg gcggcagatc gctgcgaccc
    11641 ggcaaggcct tgaacacgcc ttcgaacctg gaggagttga cgtccaccca gcccggcagc
    11701 ccgatctgct cggcgagcgc catggcctcg gcgatacgtg cctgcttgcg cgatttttcg
    11761 cgcacgctga cgacgtcgtc gggacgcacc ttgtaggacg gaatattgac gatcttgccg
    11821 ttgaccagga tcgccttgtg gttcaccagt tgcctggcct cggcgcgcgt ggagccgaag
    11881 cccatacgaa atacgacgtt atcgagccgg gtttccagca gttgcagcag attttcgccg
    11941 gtcgagccct tgaggcgcga cgcttccttg taataattgc gaaactggcg ctcgagcacg
    12001 ccgtaaatgc ggcgcaattt ctgcttttcc cgcaattgca ggccgtaatc cgactgccgc
    12061 ggcttgcgcg tgccggcgcc gtgcatgccg gggggattat cgatcttgca cttggtatcg
    12121 accgcgcggc gcgaggactt gagactcagg tccgcgccct cgcggcgcat cagtttacat
    12181 ttgggtccga tatattttgc catcttggtt cctgtctata cgcgacgttt cttcggcggg
    12241 cgacagccgt tgtgcggaat cggcgtcacg tcgtcgatac gcgagatctt gaaaccgatc
    12301 gcgttcagtg cgcgcaccgc ggaatcacgc ccggggccgg gccccttgac catcacctcg
    12361 aggtttttca tgcccatttc ctgggccgcc tggccggctc gctcggccgc cacctgggcg
    12421 gcgaacggcg tcgacttgcg cgaaccgcga aaacccgaac cgccggcggt ggcccaggtc
    12481 aacgcattgc cctggcgatc gctgatggtg acgatggtat tgttgaaagt cgcgtgaatg
    12541 tgcgcaacac cgtcggagac gacgcggcgc gctttcttct tggagcgtga tttatcgacc
    12601 atttatagtt cctgttaaat cgggcttatc gcttcagcgg gcgacgcggt cccttgcggg
    12661 tgcgcgcatt ggtcttggtg cgctgcccgc gcaccggcaa gccgcggcga tgacgaatgc
    12721 cgcgattggt tccgagatcc atcaagcgtt tgatattcat cgaaatctcg cgccgcaggt
    12781 cgccctcggt agtgtaggtg tcgacgcgca ggcgaatctg ttcgagctgc tcctcggaga
    12841 gctcgcgcac cttggtttgc ggatcgatac cggtcgcact gcaaatttcg gccgagcgcg
    12901 tcgggccgat gccgaaaatc gactgcaatg caatcacggt gtgcttgcta tccggtatgt
    12961 ttacaccagc tatacgagcc ataaaaaatc ctactcctgg gccgactggc caaaatgggc
    13021 gcaaattcta cttgccgacc gcccgaaatt caataattca atcaaggcct agccctggcg
    13081 ctgcttgtgc cgcggatcgg tacagatgac gcgcaccgag ccattacggc ggatcacctt
    13141 gcagttcttg cacattttct taaccgaagc tcttaccttc atcgttctac cctgttcccg
    13201 ggactagcgc accacaccgg cgccgccgta tcccttaaaa ttcgatttct tcatcaaacc
    13261 gtcgtactgg tgactcatca ggtgtgcctg cacctgcgac atgaaatcca tcaccaccac
    13321 gacaataatc aacaatgagg tgccgccgaa gtaaaagggc acgttccaga acaggatcag
    13381 gaactccggc agcaggcaga ccgcggtgat atagatcgcg ccgaccagcg tcagccgcga
    13441 catcacgccg tcgatgtaat tcgccgtctg gttacccggg cgaatacc
//
LOCUS       DATCZV010000461         6399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_250108, whole genome shotgun sequence.
ACCESSION   DATCZV010000461 DATCZV010000000
VERSION     DATCZV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250108"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="glk"
                     /locus_tag="VIW27_10385"
     CDS             <1..905
                     /gene="glk"
                     /locus_tag="VIW27_10385"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY5740124.1"
                     /translation="ASLAVAGHATIVDAVRAYLARSGGVKPVRAALAIALPVHGDRLR
                     MTNHHWDFSIAETREALGLESLKVINDYAALALALSRLSDSDCTQLGSGTGLPGYPRA
                     VLGPGTGLGVSGVIDGGDRWLPLESEGGHVSYGPLTAREQRVIEILHETLDHVSAEAL
                     LSGRGLASIHAALSRLAGATERLAPEQISRLALERESPIAVEALSMFCEILGTVAGNL
                     ALTLGARGGVYLGGGIAPQIRTFLQRSNFRERFEDHGRFAAYLREIPTYLIDTEYAAL
                     AGAALALGVAYDDLGVCSRATGAA"
     gene            complement(936..2537)
                     /locus_tag="VIW27_10390"
     CDS             complement(936..2537)
                     /locus_tag="VIW27_10390"
                     /EC_number="4.1.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HEY5740125.1"
                     /translation="MSRTCGEALMRLLEAYGVTTVFGIPGEHSLELYRGIQAGGLRAI
                     TPRNEQGASLMADGYARASGKPGVCILAGGSGVTNAVTGIGQANADSIPMLVISSACQ
                     GPSPRRPWERRSENLDLCEITRPITALSETVLDPEELPGLIAQAIDIFRTQRPRPVHI
                     AIPPELLEERVSEDWQATPGRGRPDPAAFEISAAADLLAGAASTLIIAGGGAQDAAAE
                     ITALAERLNAGVVMTGAGKGVVSDRSPLSLGGGMASPIVQQYLAKLEVLLAIGTELTE
                     FQYFASGLHFDGQLVRIDIDPAHIREPLPASIEVLGDARSACQQLLATLDGREIVTST
                     QDAVAGLIAVRSRQSLDYSELENRHITLLNALRSLLADDAMLFGDKTQLVYTGTSAMK
                     TFLPRSWFFPASFGTIGCALPGAIGGKVAFADRQTVALVGDGGFMFTVNELATAVEEE
                     LCIPIIVWDNHAFASIRDGMLRRGFDAIGVSPHAPDFTQLASAFGCPGTRARSLTDFQ
                     QAVRGALAHRGPSLIIVEETDDWLQ"
     gene            complement(2619..3209)
                     /locus_tag="VIW27_10395"
     CDS             complement(2619..3209)
                     /locus_tag="VIW27_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY5740126.1"
                     /translation="MIAFTLSVLLLLITPGPGVLSTAGVGAAFGFKAGLRYVAGLCLG
                     TNLVALIVVSGLAAIVLATTWARNTLLVLSALYLLSIALRIALAGKRIAFIEAARIPG
                     IRDALILQIINPKAYAVNMTWFSGFVLVPGSLVAETLIKFLVINLIWIPIHLLWLYAG
                     SSIHRLDLSPRSHSLINAGMALALVVVVALAILSLL"
     gene            3358..5226
                     /locus_tag="VIW27_10400"
     CDS             3358..5226
                     /locus_tag="VIW27_10400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEY5740127.1"
                     /translation="MTLCRLLPLLVVLLAPIPVPGAAADDQITHTLLEFVAASCRGWE
                     ASGAPAPTWGDGAFSNSDILFRGRRVGTRFRSQTGDDMRVELEVIAPGGRPTRFIGLL
                     FNAFGDPLLLAALDRECELQVMRRIDYAANGQALKIETLDADLQPLGEPDWLNPPLPP
                     AAPPPGDTHAAPPLRVGMIDSGVDYQLDEIFGRLARAADGEIVGYDFWEMDAQPYDAH
                     PVDSGFFLQRHGTRTASVLLREAPMIELVPYRYPRPDMSRMRGLIEHAAEHDVGIVGM
                     PLGSNHAEDWGEFARAARDNPQILFVASAGNNGRDIDEYPVYPASLELDNLVVVTSAD
                     DFLRPAVRTNWGRRSVDFMLPAENLPAIDYGGQPARVSGSSYAVSRLTALAARIKAAQ
                     PGLTPGELIDEMRRQWAIDSPATRRWVAGGYIADPLAGEPIARRPLPAPLLAGIDGDD
                     RLRMPLEVLVLDERWSLPRVAGMMRSAYDILAQCGIGSGDLSVYAVDAPDYLRDLSTG
                     SARTLLEAAGGDVLTVVLARDTRMLAAFTGEAFGIANTAMRPWLTNSVWLMLDVDDPG
                     IALAHEIYHVLANNGDHVEGTANLMQARTRPDSTELTPQQCQLARTNGVSNALLQR"
     gene            complement(5313..6399)
                     /locus_tag="VIW27_10405"
                     /pseudo
     CDS             complement(5313..6399)
                     /locus_tag="VIW27_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332393.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
BASE COUNT         1061 a         2119 c         2121 g         1098 t
ORIGIN      
        1 ccgccagcct ggcggtggcc ggccacgcga cgatcgtcga cgcggtgcgc gcctacctgg
       61 ctcggtcagg cggtgtaaag ccggtgcgcg cggcgctcgc tatcgcgctg ccggtgcacg
      121 gtgaccgcct gcggatgacc aaccaccact gggatttcag tatcgccgag acccgcgagg
      181 cgctgggact cgaatccctc aaggtaatca acgattatgc cgcgctggca ctggcgctgt
      241 cgcggttgag cgacagcgac tgcacgcagc tcgggtccgg caccggcctg cccggttatc
      301 cgcgggcggt actcggtccg ggtaccgggc tcggggtgtc gggcgtgatc gatggcggcg
      361 accgctggtt gccgctggag agcgagggtg gtcacgtatc ctacggcccg ctgaccgcgc
      421 gcgagcagcg ggtgatcgaa atactgcacg aaactctcga tcacgtttcc gccgaagcct
      481 tgctgtcggg ccgcggcctg gcgtcgatcc atgcggcatt gtcccgtctc gccggcgcga
      541 ccgagcggct tgccccggaa caaatctccc ggctggcact cgagcgggag tcgccgatcg
      601 cggtcgaggc attgtcgatg ttttgcgaga tcctcggcac cgtcgccggc aacctcgcgc
      661 tgaccctcgg cgcgcgcggc ggtgtttacc tcggtggcgg tatcgcgccg caaatacgca
      721 cttttctgca gcgctcgaat tttcgcgaac gcttcgaaga ccacggccgc ttcgcggctt
      781 acctgcgcga gataccgact tacctgatcg acaccgagta cgcggcgctg gccggtgccg
      841 cgctggcgct gggcgtggca tacgatgatc tcggtgtctg tagccgcgcg accggcgcgg
      901 cttagccgcc ggtcaggcgg ccgcgatcga cgtgcttact gcagccagtc gtcagtttcc
      961 tcgacgatga tcaggctcgg gccgcgatgt gcgagggcgc cgcgcaccgc ctgctgaaaa
     1021 tcggtcagcg accgcgcgcg cgtacccgga cagccgaagg cgctggcgag ttgcgtaaaa
     1081 tcgggcgcat gcgggctgac accgatcgcg tcgaaaccgc gcctcagcat gccgtcgcgg
     1141 atcgacgcga aagcgtggtt atcccagacg atgatcggaa tacagagctc ttcctcgact
     1201 gccgtcgcca gctcgttgac ggtgaacatg aagccgccgt caccgaccag cgcgacggtc
     1261 tggcgatccg caaacgcgac cttgccgccg atcgctcccg gtaaggcgca gccgatggtg
     1321 ccgaagctgg ccggaaaaaa ccagcttcga ggcaggaagg ttttcatcgc cgaggttccg
     1381 gtgtatacca gttgcgtctt gtcgccgaat agcatcgcgt cgtcggccaa caggctgcgg
     1441 agcgcattga gcagcgtgat gtgccggttt tcgagctcgc tgtaatcgag cgactgccgc
     1501 gagcgcaccg cgatcaggcc agcgacggca tcctgcgtgc tggtcactat ctcgcgacca
     1561 tcgagagtgg ccagcagctg ttggcaggcg ctgcgagcgt cgccgagcac ttcgatgctg
     1621 gccggcagcg gttcgcgaat gtgcgccggg tcgatgtcga tgcgcaccag ctggccgtca
     1681 aaatgcaggc cgctggcgaa atactggaac tcggtgagtt cggtgccgat cgccagcagt
     1741 acctcgagtt tggcgagata ctgttgcacg atcggactgg ccatgccgcc gccgagactc
     1801 agcgggctgc ggtccgaaac cacgcccttg ccggctccgg tcatgaccac gccggcattg
     1861 agccgctcgg ccagtgcggt gatctcggcg gcggcgtcct gcgctccgcc gccggcaatg
     1921 atcagcgtac tggcggcgcc tgccagcagg tcggccgcgg cgctgatctc gaacgctgcg
     1981 gggtccggcc ggccgcgtcc gggtgtcgcc tgccagtcct cgctgacacg ctcctcgagc
     2041 aattccggcg gtatcgcgat atgcaccggg cgagggcgct gggtgcgaaa aatgtcgatc
     2101 gcctgggcga tcagcccggg taattcctcg ggatcgagca ccgtttcgct gagtgccgtg
     2161 atcgggcggg tgatctcgca cagatccagg ttttcgctgc gccgctccca gggccggcgc
     2221 ggcgatggcc cctggcaggc gctggaaatc accagcatcg ggatcgagtc cgcgttggcc
     2281 tgtccgatgc cggtgacggc gttggtgaca ccggagccac ccgccaggat gcagacgccg
     2341 ggtttaccgc tggcgcgcgc gtaaccatcc gccatcaggc ttgcgccctg ctcgttgcgc
     2401 ggggtgatcg cacgcaggcc gcctgcctgt atgccgcggt agagttcgag tgaatgttcg
     2461 ccaggtatgc cgaacacggt agtcacccca taggcttcaa gcagccgcat caatgcctcg
     2521 ccgcaggttc tactcatgag ttcgcaacac cttgcccgtc aggttcattg aatgcgaaga
     2581 ttagaccata gtatcgagcc gatctaccgt gcccccggtc acagcaacga aagaattgcc
     2641 agcgccacga cgactaccag cgccagtgcc ataccggcat tgatcaggct gtgggaacgt
     2701 gggctcaggt cgaggcggtg gatgctgctg ccggcataca gccataacag gtgaatcgga
     2761 atccagatca ggttgatgac caggaacttg atcagcgtct cggcgaccag cgagcccggc
     2821 accagcacga agccggagaa ccaggtcatg ttcacggcat aggctttcgg gttgatgatt
     2881 tgcaggatca gcgcgtcacg aatacccgga attcgcgccg cctcgataaa cgcgatgcgc
     2941 ttgccggcca gtgcgatgcg caacgcgatc gacagcaggt agagcgccga caataccagc
     3001 agcgtattcc ttgcccaggt ggttgccagc acgatcgcgg cgagtcccga taccacgatc
     3061 agggccacca ggttggtgcc caggcacaga cccgcgacat agcgcaggcc ggctttgaaa
     3121 ccgaacgccg cgccgacccc ggcggtcgac agcacgccag gacccggggt aatcagcagc
     3181 agtaaaacgg acagcgtgaa ggcgatcatg ggcgggcagt gtaacccagg cggcggcaaa
     3241 atcctcgccg tgcaggacgc gtttcgaaag cgcccgcggc ggcggccgag aatcgcctgc
     3301 ccgcgggcgt cgataaacga tataatctgc gcttcttcgg tcgctcgatg tcgccctatg
     3361 accctttgcc gcctgctgcc gttgctggtc gtgttgctgg cgccgatacc ggtgccgggt
     3421 gccgccgcgg acgaccaaat cacgcatacc ctgctcgagt ttgtcgccgc cagttgccgc
     3481 ggctgggaag cgagcggcgc gccggcgcca acctggggtg acggcgcgtt cagtaattcc
     3541 gatatcctgt tccgcgggcg gcgcgtcggc acacgctttc ggtcgcaaac cggcgacgac
     3601 atgcgcgtcg agctcgaggt tatcgcgccg ggcggtcgcc cgacccgctt tatcgggctg
     3661 ctgttcaacg ccttcggcga cccgttgctg ctcgccgccc tcgatcgcga gtgcgagttg
     3721 caagtcatgc gccgcattga ttacgcggcc aacggccagg cgttgaaaat cgaaacgctg
     3781 gatgccgatc tgcagccgct cggcgagccg gactggttga atccgccatt gccacccgcc
     3841 gcaccgccgc ctggcgatac gcacgccgcg ccgccgctgc gcgtcggcat gatcgattcg
     3901 ggggtcgatt accagctgga tgagattttc gggcggctgg cgcgggccgc cgacggcgaa
     3961 atcgtcggtt acgatttctg ggagatggat gcgcaaccct acgacgcgca cccggtcgac
     4021 tcgggtttct tcctgcagcg tcatggcacc cgtaccgcat cggtgctgct gcgcgaggcg
     4081 ccgatgatcg aactggtccc ctaccgctac ccgcggcccg acatgtcgcg catgcgaggc
     4141 ttgatcgagc atgccgcgga acatgacgtc ggcatcgtcg gcatgccgct gggcagcaac
     4201 catgccgagg actggggcga gttcgcgcgc gccgcgcgcg acaatccgca gatcctgttc
     4261 gtcgcctcgg ccggcaataa tggtcgcgat atcgatgagt acccggtata cccggcgtcg
     4321 ctggagctcg ataacctggt cgtggtgacc tcggctgacg atttcctgcg ccccgcggtg
     4381 cgcaccaact gggggcgccg ctcggtcgat ttcatgctgc cggcagagaa cctgccggca
     4441 atcgattacg gcggtcaacc ggcccgcgtt tccggatcga gttatgcggt ctcacggctg
     4501 actgcgctgg cggcgcgcat caaggcggcg caacccgggt tgactcccgg cgaactgatc
     4561 gacgaaatgc ggcggcaatg ggcgatcgac agcccggcca ctcgccgttg ggtcgccggc
     4621 ggttacatcg ccgatccgct ggccggcgag ccgattgcca ggcggccgct gccggcgccg
     4681 ctgctggccg gtatcgatgg cgatgacagg ctgcgtatgc cgctcgaggt actggtgctc
     4741 gacgagcgct ggtcgctgcc ccgagtcgcc ggcatgatgc gatcggctta cgatatcctc
     4801 gcgcagtgtg gtatcggcag cggcgacctg tcggtgtatg ccgtcgacgc ccccgactac
     4861 ctgcgggatt tatccaccgg cagcgccagg accctgctcg aagccgccgg cggcgacgtg
     4921 ctgaccgtgg tgctggcacg cgacacgcgc atgctcgccg cgttcaccgg cgaagccttc
     4981 ggcatcgcca ataccgcgat gcgcccgtgg ctcaccaaca gtgtctggtt gatgctcgac
     5041 gtcgacgatc ccggcattgc gctggcccac gagatttacc acgtactcgc caacaacggc
     5101 gatcacgtcg agggcactgc caacctgatg caggcgcgca cccgccccga cagtaccgag
     5161 ttgacgccgc agcagtgcca gctggcgcga accaacggcg tcagcaacgc cttgctgcaa
     5221 agataaccgt ccgccgccgc gcgttgctcg cgagccggtt cggcagcgcc gggggtgtgc
     5281 gccggcaggc ggcgcaactt cgttcgccgt tgtcagctaa gagtctcgag cattttcgcg
     5341 cgcactcgcc aggcctggct gtcggccggc ggctcgatgt ctccgagcgt taccacctgg
     5401 cccgcgggga cgtcccttac cagtttgata tgactggcca gcccgatcgg caaggcgcgt
     5461 tgttcgacgc tggtggtggc cggcaggatg cgcccccaga ccgtgtagcc gccctcgccg
     5521 tcgagggttt cgccggcttg caggtcgcgc ttggcgaccg cggcgacgtc ggcattgaac
     5581 tgcctggcgg taccggtcgc gcggccgtcg agcgccgccg acaggatcga aacattcagt
     5641 tccagcccga tcaggtgaaa cggcttgtac atcgccgagt agcggccgct ggcatcggta
     5701 ttcatgccgt attgccgaaa acaggccgcg gtgtaatcgt tgggcgcctc gaacacgcag
     5761 taaacgcccc agcgcaggtc gcgcggcacc ggccgtccgt cgcgctcgag actggagacg
     5821 acctcgacct ggccgtcgaa ttcgagctgg ccgccggcac tacgcggccg cagcacgtga
     5881 gcgaggtcgt cgaccccgca gggcgggaaa gcgagcccac cagacggtgc ctgcaggtcg
     5941 gcggcgttgg cgatcgcggt catctcgatc gatgacttgg taccgtcgag aaacgagttg
     6001 aacatctggc tgcgcatgcc tgcagcctcg gcttcctctg cgctcagccc gtaatacgat
     6061 cacacggtgt ccggcgtcga cgcgtgatac tcgggcaggt acttggtgcc cttgccggcg
     6121 gcgacgacgc gaaagccgca gcagcgcgcc cagtcgatca tttccatcac cagcgccggc
     6181 tggtcgccgt aggccatcga gtaaacgaca ccggcactgc gcgcctgcgc ggcgagataa
     6241 ggcccggcga gcacgtcggc ctcgacgttg accatcacca cgtgcttgcc gtgctcgacg
     6301 cacagcaatg catgccgtat gcccaccgcc gggtcgccgg tcgcctcgac cacgacctcg
     6361 acgtcgtcgg cggcgatcag cgccgcggcg tcgtcgcta
//
LOCUS       DATCZV010000462         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_143411, whole genome shotgun sequence.
ACCESSION   DATCZV010000462 DATCZV010000000
VERSION     DATCZV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143411"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VIW27_10410"
     CDS             complement(<1..1253)
                     /locus_tag="VIW27_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009704824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY5740128.1"
                     /translation="MTTYFIQAFIYLCAAVVAVPLARRFGLGSVLGYLIAGVIIGPVI
                     GLVGEETATLQHFAEFGVVMMLFLVGLELEPKMLWSMRQRLLGLGGLQVCLTTAAIMA
                     AGLVFGLDWRLALSIGLILALSSTAIVLQTLNEKGLTRTEGGRASFSVLLLQDIAVIP
                     MLALIPLLELPELAEAARHAAVAAADAHANGHLDLVAGLPNWLYALVVLAAVAAVVLG
                     GHFLSRPLFRFVAQSGMREVFTATALMLVIGIAALMSLVGLSPALGTFLAGVVLANSE
                     FRHELETNIEPFKGLLLGLFFITVGAGVDFGVLADAWLLVGGLAIAVILLKAAIVYAL
                     AAIFGIGSSDRWLAALGLAQAGEFGFVLLSFSRQNNVVPSDLAELLALVVAISMFLTP
                     ALFIFFDKVLLPRFPANAAQRYPDP"
     gene            complement(1250..1864)
                     /locus_tag="VIW27_10415"
     CDS             complement(1250..1864)
                     /locus_tag="VIW27_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5740129.1"
                     /translation="MPASILILFAHPSQHRSEVNRPLLDASRRQQAVTVVDLYAEYPD
                     YCIDIDREQRRLREHDVIVFMFPLFWYSTPAILKEWQDLVLEYGFAYGHDGKQLHGKQ
                     LLCAVTAGGIEDAYRSRGYNHYTIRELLRPIEQTATLCGMQYLPPYALFGARTALEDG
                     RVDAHIDNWVQILDCLRDGRVDGERAAGAERLDDCVTALDGEQS"
     gene            2395..2766
                     /locus_tag="VIW27_10420"
     CDS             2395..2766
                     /locus_tag="VIW27_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740130.1"
                     /translation="MRKIEGNVRSYTPINVRNVQFEIHGSNLVIDLCMDQPWVLKNGD
                     HIAVVGEDDGRSGTFHGYAYCNHTRKICGQTDRGLADALRYLFLGLLFSWAIFPIFVH
                     LPYGWRLLAFGRKVDQAAAML"
     gene            complement(2780..>3408)
                     /gene="amrS"
                     /locus_tag="VIW27_10425"
     CDS             complement(2780..>3408)
                     /gene="amrS"
                     /locus_tag="VIW27_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY5740131.1"
                     /translation="RTVAVTAGYIDPEPRAEFFAAMDAANVDLKAFTERFYPKICGGH
                     LAPVLETLEFIRQHTDTWLELTTLLIPGENDSDDELHRLSAWVAEKLGADVPIHFTAF
                     HPDWKMRDIARTPAATLTRARRIALQNGLRYAYTGNVHDSEGSSTWCHACGALLIERD
                     WYVLGRWGLDSHGCCAKCGERLPGHFLERPDSFGARRIPVRLDGSLGV"
BASE COUNT          682 a         1023 c         1129 g          574 t
ORIGIN      
        1 atcgggtcgg gataacgttg cgctgcgtta gccgggaagc gcggcagcaa taccttgtcg
       61 aagaaaatga acagcgccgg cgtcaggaac atggaaatgg cgacaaccag cgccagcagc
      121 tcggccaggt cgctcggcac cacgttgttc tggcggctga agctcagcag cacgaagccg
      181 aattcaccgg cctgtgcgag tccgagggcc gccagccagc ggtcgctgga accgatgccg
      241 aagattgccg ccagcgcgta aacgatggct gccttgagca ggatgacggc gatcgccagg
      301 ccaccgacca gcagccacgc gtcggccagc acgccgaaat cgacgccggc gccgacggta
      361 atgaagaaca ggccgagcaa caggcccttg aacggttcga tattggtttc cagttcgtgg
      421 cggaactcgc tgttggcgag taccacgccg gcgagaaaag tgccgagcgc cggcgacagg
      481 ccgaccaggc tcatcagcgc ggcgatgccg atcaccagca tcagcgcggt ggcggtgaat
      541 acctcgcgca tccccgactg ggcgacgaag cggaacagcg ggcggctgag aaagtgcccg
      601 ccgagcacca cggcggcgac cgcggcgagc acgacgagcg cgtatagcca gttgggcagt
      661 cccgcgacca ggtcgaggtg gccgttggcg tgggcgtcgg cggcggcgac cgcggcgtgc
      721 cgggcggcct ctgccagttc cggcagttcc agcagcggga tcaacgccag catcgggatt
      781 accgcgatgt cctgcaacag taaaaccgag aacgaggcgc gcccgccctc ggtgcgggtc
      841 aggccttttt cgttgagggt ttgcagcacg atcgcggtcg acgacaacgc caggatcagg
      901 ccgatggata acgccagccg ccagtcgagg ccgaagacca ggccggcggc catgattgcg
      961 gcggtggtca gacagacctg caatccgccg agaccgagca gccgctggcg catcgaccag
     1021 agcattttcg gttccagttc cagtccgacc aggaacagca tcatcaccac gccgaactcg
     1081 gcgaaatgct gcagggtcgc ggtttcctcg ccgaccaggc caataaccgg gccgatgatg
     1141 acgccggcga tcaggtagcc gagcaccgag ccgagaccga agcggcgcgc cagcggtacc
     1201 gcgaccaccg ccgcgcacag gtagatgaag gcctggatga aataggtcgt catgactgtt
     1261 caccgtcgag cgcggtgacg caatcgtcga gccgctcggc gccggcggcc cgctcaccgt
     1321 cgacgcggcc gtcacgcagg cagtcgagga tttgtaccca gttgtcgatg tgggcatcga
     1381 cgcggccgtc ctcgagcgcg gtgcgcgcgc cgaacagcgc gtagggcggc aggtactgca
     1441 tgccgcacag ggtcgcggtc tgctctatcg ggcgcagcag ttcacgaatc gtgtaatggt
     1501 tgtagccgcg gctgcggtag gcgtcctcga tgccgccggc ggtgaccgcg cagagcaatt
     1561 gcttgccgtg cagttgcttg ccgtcgtgcc cgtaggcgaa accgtactcg agcaccaggt
     1621 cctgccattc cttgagaatc gccggggtcg aataccagaa cagcggaaac atgaagacga
     1681 tgacgtcgtg ctcgcgcagc ctgcgctgtt cacggtcgat atcgatgcag taatccgggt
     1741 actcggcata caggtcgacg acggtaacgg cctgctgccg gcgcgaggcg tcgagcagcg
     1801 gccggttgac ctcggagcgg tgctgagatg ggtgcgcgaa caggatcagg atgctggcgg
     1861 gcatggatcg ggaccggatc gaaaatgaca tggccctgta taaagactag ccggcaatta
     1921 atcaagccag gcgattcgcc agtgtcggtg aaccgccagg ccggcaatcc gctgctgtct
     1981 gcgtgcctca aaacggggca cgcgatcact ccacgaggct gcggccggcg atcgctggtg
     2041 gcagttttcg gcgcctggcc ggcagtcatc gagcggccag gatcgagcgc tatagcggca
     2101 gcccgtcggg tggagaaaat tgtattttcg agggtctgaa aagcggttga aaatggcgtc
     2161 cgggaagtga tattgtgcac tgcagcacaa ttttgcagaa tgttgctcgg aaagtgcgta
     2221 aatagttcat tttcaggcag aaaatggcaa gtttttcgtc tataaacggt cgacaagagg
     2281 gctcgaaatg cggtacaaag cgcaccgtgt ccggcgcctt ccgggggctc gaaaaaggct
     2341 tgaattttcg gcccgcgacc atagcttgca agtgttcgaa acagggtgac agcaatgcgc
     2401 aaaatcgagg gcaatgttcg gagttacacg ccgatcaacg tccgcaacgt ccagtttgaa
     2461 atccatggca gtaacctggt catagacctg tgcatggatc aaccctgggt gttgaaaaac
     2521 ggcgaccata tcgccgtggt tggcgaggac gatggccgtt ccggcacgtt tcatggctat
     2581 gcctactgca atcacacgcg caagatctgc gggcagaccg atcgcggcct ggccgacgcg
     2641 ctgcgctacc tgttcctcgg gttactgttt tcgtgggcga ttttcccgat tttcgtccat
     2701 ctgccttacg gctggcgcct gctggctttc ggccgcaagg tcgaccaggc cgcggcgatg
     2761 ctctaaccgc ggccgccgct cagactccca gggaaccgtc gaggcgcacc ggtatgcggc
     2821 gcgcgccgaa gctgtcgggc cgctcgagaa aatgtccggg cagccgctcg ccgcatttcg
     2881 cacagcagcc gtggctgtcc agtccccagc ggccgagcac gtaccagtca cgctcgatca
     2941 gcagcgcgcc gcaggcgtgg caccaggtac tggatccctc gctatcgtgc acgttgccgg
     3001 tgtaggcgta acgcaacccg ttttgcagcg cgatgcgacg cgcgcgcgtc agcgtcgcgg
     3061 ccggggtgcg cgcgatgtcg cgcatcttcc agtcgggatg aaacgcggtg aaatgaatcg
     3121 gtacgtcggc gccgagtttt tccgctaccc aggcgctgag gcgatgcaat tcgtcgtcgg
     3181 agtcgttctc gcccgggatc agcagcgtgg tcaactcgag ccaggtatcg gtgtgctgcc
     3241 gaataaactc gagggtttcg agcaccggcg ccaggtggcc gccgcaaatc ttaggataaa
     3301 aacgctcggt aaacgctttc agatcgacat tggcggcatc catcgcggca aagaactcgg
     3361 cgcgtggctc gggatcgata tagcccgcgg taaccgcgac cgtgcgaa
//
LOCUS       DATCZV010000463         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_355935, whole genome shotgun sequence.
ACCESSION   DATCZV010000463 DATCZV010000000
VERSION     DATCZV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355935"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VIW27_10430"
     CDS             complement(<1..665)
                     /locus_tag="VIW27_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY5740132.1"
                     /translation="MRFRTLNPDGAAGALLESVNDKAGNDKPVNSTFGSAAYGGEHWS
                     PRQASHAQENGHVWRTCGIDSEWRRLEAVLLHCPGEELHVSLENPEAVQMLESVDLGR
                     AREEHQAMVEAYRAQQVQVHYVEPEQPCQPNLMFCADLFVMTPQGAILARPASTVRAG
                     EERQVARRLAELGIPLLKTLTGNAVFEGADLMWIDANTAIVGRGLRTNDAAIAQLANL
                     FAEL"
     gene            726..1112
                     /locus_tag="VIW27_10435"
     CDS             726..1112
                     /locus_tag="VIW27_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEY5740133.1"
                     /translation="MIVRTLDEIIDTPREVRGEVWASRRFLLADDGLGFTLTETTVDA
                     GADQVLWYKHHIEANYVIHGEGEVENLVTGEVFPLLPGTIYVLDRHERHRLRAFTRLR
                     LVCVFTPPLTGAETHDADGAYAPPAG"
     gene            complement(1155..1595)
                     /locus_tag="VIW27_10440"
     CDS             complement(1155..1595)
                     /locus_tag="VIW27_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HEY5740134.1"
                     /translation="MTWNVYLSGEIHSDWRQQIIDGAGKLGLAVNFSSPVTDHDASDA
                     AGDMLGPESGGFWRDHKSSKVNAIRTKTLLEKCDLAVVRFGDKYRQWNAAFDAGYCAA
                     IGKPYITLHAADIVHPLKEVDATAMAWAETPAQVVEILQYVIDA"
     gene            1737..>2382
                     /locus_tag="VIW27_10445"
     CDS             1737..>2382
                     /locus_tag="VIW27_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740135.1"
                     /translation="MPLFRRLLLALSLAAGSLLPVLSAAQEMARSGPDLSIEMLENRL
                     REVEASNELEGADKEALLDLYRRSISLIEQRRGYQSATGEFAEARKSAPADTTRLREQ
                     LNAIESSEVAKLQDSLLRMELPELEQHLLSEKADLAGLAASLAEVESRRDTQLNRGQQ
                     ARERVNEARKRQLEIADELQAPVSETQPQRITEARRWVLQLEHRTLGAEIEMLEQ"
BASE COUNT          425 a          736 c          767 g          454 t
ORIGIN      
        1 agttcggcga acaggtttgc gagttgcgcg atcgccgcat cgttggtgcg cagtccgcgg
       61 ccgacgatcg cggtattggc atcgatccac atcagatccg cgccttcgaa cacggcatta
      121 ccggtcaggg ttttcagcag cggaataccg agctcggcga gccggcgggc gacctggcgc
      181 tcctcgccgg cgcgcactgt cgacgccggc cgcgcgagga tcgcgccctg cggcgtcatc
      241 acgaacagat cggcgcaaaa catcaggttg ggctggcagg gttgctcggg ttcgacgtaa
      301 tggacctgca cctgttgcgc ccgataagcc tcgaccattg cctggtgctc ttcgcgcgcg
      361 cggccgaggt cgacgctttc gagcatttgc acggcctcgg gattttccag cgagacgtgc
      421 aactcttcgc cggggcagtg cagcagcacc gcttcgagcc gccgccattc cgaatcgata
      481 ccgcaggttc gccagacgtg gccgttttcc tgcgcgtgcg aagcctggcg cggcgaccag
      541 tgttcgccac cataggcggc gctgccaaag gtgctgttga cgggtttgtc gttaccggcc
      601 ttgtcgttca cggattccag cagggcgccc gcggcgccat cggggttgag agtccggaac
      661 cgcaactcgg ttccgccgcc aggctttgat gctatcatgt tcgatcgtag caaaggtagg
      721 ccaccatgat cgttcgaacg ctcgacgaaa ttatcgatac gccgcgtgag gtgcgcggtg
      781 aggtctgggc cagccggcgt ttcctgctcg ccgatgatgg cctcggcttc acgctcaccg
      841 agaccacggt cgatgccggc gccgaccagg tgctgtggta caagcatcac atcgaggcga
      901 actatgtgat ccatggcgag ggcgaggtcg aaaacctggt cacgggagag gtttttccgc
      961 tgctgccggg cacgatttac gtgctcgacc gccacgagcg tcaccggctg cgcgcgttca
     1021 cgcgcctgcg cctggtgtgc gtgttcacgc cgccactgac cggtgccgaa acccatgacg
     1081 cggacggtgc ctacgcgccg cctgccggct gagtgccggc gatccggccc ggtgttttct
     1141 gcggcgccgc gcgctcaggc gtcgatgacg tattgcagga tttcgaccac ctgcgccgga
     1201 gtttcggccc aggccattgc cgtcgcgtcg acctctttca gcggatgcac gatatcggcc
     1261 gcgtgcagcg tgatataggg cttgccgatc gctgcgcaat agccggcgtc gaaagccgcg
     1321 ttccactgcc ggtacttgtc gccaaagcgc accaccgcca gatcgcattt ttcgagcagg
     1381 gtcttggtgc gtatcgcgtt gaccttcgac gacttgtggt cgcgccagaa gccgccggac
     1441 tcgggtccga gcatgtcgcc ggcggcgtcg ctcgcgtcgt gatcggtcac cggcgagctg
     1501 aagttcaccg ccagcccgag tttgccggca ccgtcgatta tctgctggcg ccagtcgctg
     1561 tgaatttctc ccgatagata tacattccag gtcatggaat tctccatttt gaatggcagg
     1621 cgccgaaggt cgcctgccgc aatcgaaaat attcgcgagt caccattatt taacaattac
     1681 gccggcaaag acagtgataa gatggcgtca ttccgaatcg acctgcaggg ctgaaattgc
     1741 ctttatttcg cagactattg ctcgccttgt cgctcgccgc cggcagcctg ttgccggtgt
     1801 tgtcggctgc ccaggaaatg gcgcggagcg gccccgacct cagtatcgaa atgttggaaa
     1861 accggttgcg ggaggtcgaa gcctcgaacg aactcgaagg tgccgacaag gaggccttgc
     1921 tcgatcttta ccgcagatcg atcagtctca tcgaacagcg gcgcggttac cagtcggcaa
     1981 ccggtgaatt tgccgaggcg cgcaagtcgg cgcccgccga caccactcgc ctgcgcgagc
     2041 aactgaatgc catcgagtcc agcgaggtgg caaagttgca ggactcgctg ttgcgtatgg
     2101 aattaccgga gctcgagcag cacctgctca gtgaaaaggc ggatctggcg gggctcgcgg
     2161 ccagcctggc ggaagtcgag agccgccgcg atacgcagct caatcggggc cagcaggctc
     2221 gtgagagggt caacgaggca cgcaagcggc agctggagat tgccgacgag ctgcaggcgc
     2281 cggtatcgga aacccagccc cagcgcatta ccgaggcgcg gcgctgggtg ctgcaactgg
     2341 aacatcgtac gctgggcgcc gagatcgaaa tgctcgagca gg
//
LOCUS       DATCZV010000464         3983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_143651, whole genome shotgun sequence.
ACCESSION   DATCZV010000464 DATCZV010000000
VERSION     DATCZV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143651"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VIW27_10450"
     CDS             <1..725
                     /locus_tag="VIW27_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5740136.1"
                     /translation="SSHCIGMCGGIVSALSLGIGDDLQRQPGRLLAYHLAYNGGRIAS
                     YLLVGLLAGSLGGGLLELGVNPAAGKLLAAAFMIALGLYLANWWRGLTLLERLGYRLW
                     RHIRPLGEKLMPVRSLPQAALLGMLWGWLPCGLVYAAVAWSLTAGNARDGALLMLGFG
                     IGTLPALLAAGNAMHYLQHWVRSPRVRASAGAMIIALGIYTGYSAFAANTHHGHSTAW
                     LRPALPASPFGSIRAPAILRRV"
     gene            complement(641..1396)
                     /locus_tag="VIW27_10455"
     CDS             complement(641..1396)
                     /locus_tag="VIW27_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5740137.1"
                     /translation="MQYKAQICHMVHKCLICDPRGGCLGLQLLNSPGISDHLKQVKRI
                     SRKGEHLFREGEIADAFFVVRSGSVKSYLLTEDGEEQVLGFYLPGDIFGLDNTDSQYR
                     LSSAVTLETTSICRFPHAYLSEKARGANLLRITAEQMQRDHSLVLMLARKDADGRIAT
                     FIDDLASRYWSRGYSGTSFVLTMSRQDIGCYLGLAVETVSRTLTRFQESGILRVNRRE
                     VEILDHQTLRRIAGARIEPKGEAGKAGRSQAVE"
     gene            1539..2756
                     /locus_tag="VIW27_10460"
     CDS             1539..2756
                     /locus_tag="VIW27_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5740138.1"
                     /translation="MKPSRASGRLFVIVTLAALLVGAITILIIYVFPAQDRSGAVLLT
                     SGLLLLLLAPLAGLTVSALSLHPPNQEPQDLPYQSQIFRILAEAAREGIVLADTAGRI
                     EFVNPAMEQLFGYAQGELLGKSVERLMPEEYRSVHGGFMQRYLQTGEGRIVGTGRQVT
                     ALDSKGRRFPIYLSIGDIDTGSRRLFAGVMLDMSEQQELQREILEIPINEQRRIGQEL
                     HDGLGQQLTGLAMLATSLLNKASKPDYELAAQLAKGLQEAVSQVRALSRGLVPVEVEA
                     GDLIHALEGLVDNLRRQTGLDISLQVGERLGIRDSSCAVHVYRIAQEALNNAIKHAGA
                     SLIQVFIGREGDRGLLSITDDGSGFDLQQESATGLGMRIMKYRCELIDARLEVETPPA
                     EGCRIKCYFALDR"
     gene            2773..3414
                     /locus_tag="VIW27_10465"
     CDS             2773..3414
                     /locus_tag="VIW27_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5740139.1"
                     /translation="MARILIVDDHPLVRTGLAQLIGDCPDLEVCGEAGDMAEGLRRVD
                     ELQPDLAIIDLSLAGGSGLDLIEHIKARDKGTLMLVASMHDEALYAERVLAAGARGYV
                     NKQEAQDRIIFAIRRVLGGKVYLSERMTERLLSGIDESSSEKRDIDTLSNRELQVFEL
                     IGQGVPTSRIAEQLNLSTKTIETHQAHIKRKLGLDSAHQLNQRAIRWVMAGDD"
     gene            3502..3948
                     /locus_tag="VIW27_10470"
     CDS             3502..3948
                     /locus_tag="VIW27_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY5740140.1"
                     /translation="MNMQVWNPFQEFENLLDRYSKAGGNRLGKQLDSEMSFADWAPSV
                     DIEEAEDKYVIKADLPGVEKKDIDVKLENGVLSIRGEKNVEKETGKDTRHHRRERFHG
                     TFARSFTLPDAVKAEAVDASYRDGVLTLYIPKKEEAKPRSIDIKVN"
BASE COUNT          788 a         1235 c         1219 g          741 t
ORIGIN      
        1 gctcgagtca ctgtatcggc atgtgcggcg gcatcgtgtc cgcgctgagc ctcggtatcg
       61 gcgacgacct gcagcgacaa cccggacgcc tgctggcata tcacctcgct tacaacggtg
      121 gacgtatcgc ttcctacctg ctggtcggcc tgctggcggg ctcgctcggc ggcggcttgc
      181 tggaactcgg tgtgaatccg gcagccggca agttgctggc agcagccttc atgatcgcgc
      241 tcggtctcta cctcgccaac tggtggcgcg gcctgacgct gctggaacgg ctcggctatc
      301 gcctgtggcg gcatattcgc ccactcggtg aaaaactcat gccggtgcgc agcctgccgc
      361 aggcggcgct gctcggcatg ctctggggct ggctgccctg cgggctggtt tacgccgcgg
      421 tcgcctggtc gcttaccgcg ggcaatgcgc gcgacggcgc gctgctgatg ctcggctttg
      481 gtatcggtac gctgccggcg ttactggccg ccggcaatgc catgcactac ctgcagcact
      541 gggtgcgttc gccgcgagtc agggcctcgg cgggagcgat gatcatcgca ctgggcatct
      601 acaccggtta ctcggcgttc gccgccaaca cccaccacgg tcactcgacg gcttggctgc
      661 ggcctgcctt gccggcctct cccttcggct cgatacgcgc accggcgatc ctgcgcaggg
      721 tttgatgatc gagtatttcg acctcgcggc gattgacccg caggatgccg gactcctgga
      781 agcgcgtcag cgtgcggctc acggtctcga ccgccaatcc caggtagcag ccgatatcct
      841 ggcgggacat cgtcagcacg aatgaggtcc cggaatatcc gcgcgaccag tagcgcgacg
      901 ccagatcgtc gataaacgtc gcgatgcgcc cgtcggcgtc cttgcgcgcc agcatcagca
      961 ccaggctgtg atcgcgctgc atctgctcgg cggtgatgcg cagcagattg gcgccgcggg
     1021 ccttctcgga caggtaggcg tgcggaaacc gacagatcga ggtcgtttcc agcgtgaccg
     1081 ccgaagacag tcgatactgg gagtcggtat tgtcgagtcc gaagatatcg cccggcaggt
     1141 agaatccgag cacttgttcc tcgccgtcct cggtcagcag gtaactcttc accgagccac
     1201 tgcgcaccac gaagaaggca tcggcgatct caccctcgcg aaacaggtgt tcgcccttgc
     1261 ggctgatgcg ctttacctgc ttcaagtggt cgctgatacc aggactgttc agcaactgca
     1321 aacccaggca tcccccccgc ggatcgcaga tgaggcactt gtgtaccatg tgacagatct
     1381 gagccttgta ttgcattgtc atagctccag tatagtaaat tcgtgcggtt attgtgcgtc
     1441 actaaagctt cggatggtat cggggttttc ctgatgggca tatcgaggct ttcgagataa
     1501 catgtgacta ctcgctagct gggaaatatc gagccgcgat gaaaccatcc cgggcctctg
     1561 gccgattatt cgtcatcgtg acgctggcgg cgctgctggt cggcgcaatc acgattttga
     1621 ttatttacgt tttccctgcc caggaccgct ccggcgcggt tttgctgact tcaggattgc
     1681 tactgctgct gctcgcgccg ctcgccggcc tcacggtcag cgcgctgtcc ctgcacccgc
     1741 cgaaccagga gccgcaagat cttccctacc aatcgcaaat cttcaggata ctcgccgaag
     1801 cggcgcgcga gggcatcgtg ctcgcggata ccgccggcag gatcgagttc gtcaatccgg
     1861 ccatggaaca attgttcggc tatgcgcagg gcgaactgct gggtaagtcg gtcgagcgac
     1921 tgatgcccga ggagtaccgc tcggtccacg gcggtttcat gcagcgctac ctgcaaaccg
     1981 gcgagggtcg aatcgtcggt accggccggc aggtaaccgc gctcgacagc aagggcaggc
     2041 gttttccgat ttatctgtcg atcggcgata tcgataccgg cagccgccgg ctgttcgccg
     2101 gcgtgatgct cgacatgagc gagcagcagg aattgcagcg cgagattctg gagatcccga
     2161 tcaacgagca gcgccgcatc gggcaggagc tgcacgacgg tctgggccag cagctgaccg
     2221 gacttgcaat gctggccacc agcctgctca acaaggccag caagcccgac tacgaactcg
     2281 cggcgcaact cgccaagggc ctgcaggagg cggtttcgca ggtgcgcgca ctgtcgcgcg
     2341 gtctggtgcc ggtcgaagtc gaggccggtg acctgatcca cgccctcgag ggcctggtcg
     2401 acaacctgcg ccggcaaacc ggtctcgaca tctcgctgca ggtaggcgaa cgcctgggca
     2461 tccgcgatag cagctgcgcg gtgcacgtct atcgaatcgc tcaggaggcg ctcaacaatg
     2521 ccatcaagca tgccggcgcc agccttatcc aggtgttcat cggccgcgaa ggggaccgcg
     2581 gcctgctgtc gataaccgac gacggctcgg gcttcgacct gcagcaggag agcgctaccg
     2641 gtctcggcat gcgcatcatg aagtaccgtt gcgaattgat cgatgccagg ctggaagtcg
     2701 aaacgccccc tgctgaaggt tgccggatta aatgttattt tgcgctcgac cgttaaccgc
     2761 agaggcaggc caatggcacg aatcctgatc gtcgacgacc acccgctggt acgcaccggt
     2821 ctcgcgcaac tgatcggcga ctgccccgac ctcgaggtct gcggcgaagc cggcgatatg
     2881 gccgagggcc tgcgccgggt cgacgagctg caaccggacc tggcgatcat cgatctgtcg
     2941 ctggccggcg gcagcggcct ggacctgatc gagcacatca aggcccgcga taagggcaca
     3001 ttaatgctgg ttgcctcgat gcatgacgaa gccctgtacg cggagcgcgt gctggcagcc
     3061 ggcgcgcgcg gctatgtcaa caagcaggaa gcccaggatc gaatcatttt cgcgatacgc
     3121 cgggtgctcg gaggcaaggt ttacctgagc gagcgcatga ccgaacgatt actctccggc
     3181 atcgacgagt caagcagcga aaagcgcgac atcgacaccc tctccaaccg cgagctgcag
     3241 gtgttcgaac tgatcgggca aggtgtaccc accagccgga tcgccgagca gctgaacctc
     3301 agcaccaaaa cgatcgagac ccaccaggcg catatcaaga gaaagcttgg tctcgattcc
     3361 gctcaccagt tgaaccagcg tgctattcgc tgggtaatgg caggagacga ctgaaatcgc
     3421 catcctatct tgatccggat caagaattcc ccggttgccg accgtaaaat ttaactggtg
     3481 acagatacga gggagacagc catgaacatg caagtatgga atccgtttca agagttcgaa
     3541 aatctgctgg accgttacag caaagccggt ggcaaccggc tcggcaagca gctcgattcc
     3601 gagatgagct ttgccgactg ggcgccttcc gtcgacatcg aggaagccga agacaagtac
     3661 gtgatcaagg ccgacctgcc cggcgtcgag aagaaggata tcgacgtcaa gctcgaaaac
     3721 ggcgtactca gcatccgcgg tgaaaagaac gtcgagaagg aaacgggcaa ggatacccgc
     3781 caccatcgcc gcgaacgctt tcacggcacc ttcgcgcgca gcttcacact gcccgacgcg
     3841 gtcaaggccg aagccgttga cgcctcttac cgtgacggcg tgctgaccct gtacattccg
     3901 aaaaaggaag aagccaagcc gcggtcgatc gatatcaaag tcaactaaat acccgtcaag
     3961 gatgacattc gccgagcggc atc
//
LOCUS       DATCZV010000465         3992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_90798, whole genome shotgun sequence.
ACCESSION   DATCZV010000465 DATCZV010000000
VERSION     DATCZV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90798"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="aat"
                     /locus_tag="VIW27_10475"
     CDS             complement(<1..500)
                     /gene="aat"
                     /locus_tag="VIW27_10475"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY5740141.1"
                     /translation="MIPWLEPTAIPRFPDTASALAEPRGLLAAGGRLDVEWLLVAYRQ
                     GIFPWFSENEPILWWSPAPRTVLFPAGFHHSRSLQKLARREKYRISRNRDFGSVIRAC
                     AEQRAGQQGTWINAQMIAAYTEMHRAGFAQSIECWDDERLVGGLYGIALGRVFFGESM
                     FSREANT"
     gene            complement(546..1721)
                     /locus_tag="VIW27_10480"
     CDS             complement(546..1721)
                     /locus_tag="VIW27_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5740142.1"
                     /translation="MPGKWSNAETRVGVSHSLSEIPAAQWDALGEAGYPFTRHAFLYG
                     LECHGCLEPFGWYPAYFHLHAGVQLLAAIPCYIKTNSYGELVFDHAWVHAYERHGLDY
                     YPKLVTAIPYTPATGPRFLVNRELVDDAADHEHLRDLLCRAIQGFCSEQKLSSWHILF
                     ADKPLLDALEPRDIMPRSDVQFHWSNRDYPDFAAFLAELSSRKRKNIRKERASAAAHD
                     LDIGMHRGESLSAADWQRIHALYAGIYRRKYGTATLTADFFEHLGDAMPAQVLAALAR
                     ERGRIVAASLFFSSDSHLYGRVWGCDGYFDHLHFECCYYQGIDYCIEQGLACFDPGAQ
                     GEHKLARGFLPTLTWSGHWIAHPQFREAIANFLQQERNHIRAYQAGLLEHSPYRHAK"
     gene            complement(1702..2661)
                     /gene="trxB"
                     /locus_tag="VIW27_10485"
     CDS             complement(1702..2661)
                     /gene="trxB"
                     /locus_tag="VIW27_10485"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY5740143.1"
                     /translation="MSAIKHRNVIILGSGPAGYSAAVYAARANLSPMVITGVEAGGQL
                     MTTPDVDNWPGDVEGLTGPVLMQRMQQHAERFETEIVFDQVHTVHLDEEPKRLIGDSG
                     EYSCDALIVATGASAMYLGVPSEEKFHGKGISACATCDGFFFRGQKVAVIGGGNTAVE
                     EALYLSNIASEVILVHRRDKLRAEKIMQDQLFEKERSGNVRIAWNSVFDEALGDDSGV
                     TGLRIRNVQDDTTRELELSGIFIAIGHQPNTGIFEGQLEMKNGYIVVHSGLDGNATAT
                     SVPGVFAAGDVMDHVYRQAVTSAGSGCMAALDAEKYLDARKME"
     gene            3013..>3992
                     /locus_tag="VIW27_10490"
     CDS             3013..>3992
                     /locus_tag="VIW27_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY5740144.1"
                     /translation="MQAKLKSRADDEQKGRMFAGHVGQRLREAGMLLSFALAGYFLLA
                     MFSYDALDPSWSHSGSNSEIANFGGAAGAWVADLLFYLFGYLAYLLPIMLIYNGILLV
                     RSRDLSAEAQYHMLVIRWSGFVLTLISGSALSSLHFVVVPGSMPLDAGGILGQITGNY
                     FSQGLGFIGATVMHLALFLAGLTLFTGLSWLALVDNTGRCTLLAIDGLLDRYYLLRDR
                     FEGKRHRSERAQVFQVQQKKQETRKPPKIEPVVEKIEPSPRVEKEKQIPLFSPGGNGK
                     GSIPPLALLDKPRPRVAAYSKSALEAMSRQLELKLLDFGIEIQVESVHPGP"
BASE COUNT          721 a         1196 c         1288 g          787 t
ORIGIN      
        1 gtattggctt cgcggctgaa catcgattcg ccgaagaata cgcggccgag cgcgatgccg
       61 tagaggccgc cgaccagccg ctcgtcatcc cagcactcga tcgattgcgc gaacccggcg
      121 cggtgcatct cggtgtaggc agcgatcatc tgtgcattga tccaggtccc ctgctggccg
      181 gcacgttgct cggcgcaggc ccggatcacg ctgccgaaat cgcggttgcg ggagattcgg
      241 tatttttcgc ggcgcgccag tttctgcagg ctgcggctgt ggtgaaagcc ggcggggaat
      301 agcaccgtgc gcggcgccgg tgaccaccac aggatcggtt cattttcgct gaaccagggg
      361 aaaataccct ggcggtaggc gaccagcagc cattcgacat ccagccggcc gcccgcggcg
      421 agcagcccgc ggggctcggc cagcgccgag gcggtatccg gaaagcgcgg aatcgcggtg
      481 ggttctagcc agggaatcac tatttaaaac aataatttat tttattaatt taaagtaatc
      541 tttcatcatt tcgcgtgacg gtatggcgaa tgctcaagca agcctgcctg gtaggctcgt
      601 atatggtttc tttcctgttg cagaaagttc gcgatcgcct cgcggaactg cgggtgcgcg
      661 atccagtggc ccgaccaggt cagggtcggc aggaatcccc gcgcgagctt gtgctcgccc
      721 tgcgcgccgg ggtcgaagca tgccaggccc tgctcgatac agtagtcgat cccctgataa
      781 taacagcatt cgaaatgcag gtgatcgaag taaccgtcgc aaccccagac gcgcccgtag
      841 aggtgactgt cactgctgaa gaacagcgac gcggcgacga tccggccgcg ctcgcgggcc
      901 agcgcggcca gtacctgtgc gggcatggca tcgcccagat gctcgaagaa atcggcggtc
      961 agggtcgcgg tgccgtactt gcgacgatag ataccggcgt aaagcgcgtg gatgcgctgc
     1021 cagtcggcag cgctcagtga ctcgccgcga tgcatgccga tatcgagatc gtgcgcggcg
     1081 gcgctggcgc gctccttgcg gatatttttg cgcttgcgtg aactgagctc ggcgagaaac
     1141 gccgcgaaat ccgggtaatc gcggttcgac cagtgaaact gcacgtcgct gcgcggcatg
     1201 atgtcgcggg gttcgagcgc gtcgagcagt ggtttgtcgg cgaacaggat atgccacgag
     1261 ctcagtttct gttcgctgca gaatccctgg atggcacggc acagtaaatc gcgcaagtgt
     1321 tcgtggtccg ccgcgtcgtc gaccagttcg cggttgacca ggaaacgcgg tccggtggcc
     1381 ggcgtgtacg gaatcgcggt taccagcttg gggtaataat cgagcccgtg ccgctcgtag
     1441 gcgtgcaccc aggcgtgatc gaagaccagt tcgccataag agttggtttt gatgtaacac
     1501 gggattgcag ccagcagttg cactccggcg tgcaggtgga agtaggccgg gtaccagccg
     1561 aacggctcga ggcagccgtg gcattcgagc ccgtagagga aggcgtggcg ggtgaacggg
     1621 tagcctgctt cgccgagcgc atcccattgc gcggcgggta tttcgctcaa cgagtggctg
     1681 acgccgacgc gggtttccgc gttactccat tttcctggca tcgagatact tttccgcgtc
     1741 cagcgccgcc atgcaaccgc tgcccgccga ggtgaccgcc tggcggtaga cgtgatccat
     1801 gacatcgccg gcggcgaaca ccccgggtac gctggtcgcg gtggcattgc cgtcgagacc
     1861 gctgtgcacg acgatgtagc cgttcttcat ttccagttga ccctcgaaga tgccggtatt
     1921 cggctggtgg ccgatggcga tgaaaatgcc ggacagttcg agttcccggg tggtgtcgtc
     1981 ctgcacgttg cgaatgcgca gcccggtgac gccggagtcg tcgccgagcg cctcgtcgaa
     2041 aacgctgttc caggcgatcc ggacgttgcc gctgcgctcc ttttcgaata actggtcctg
     2101 catgatcttc tcggcgcgca gcttgtcgcg gcgatgcacc aggatcacct cggaggcgat
     2161 gttggacagg tagagggctt cctcgaccgc ggtattgccg ccgccgataa cggccacctt
     2221 ctggcccctg aaaaagaaac cgtcgcaggt cgcgcaggcc gaaatgccct tgccgtggaa
     2281 cttttcctcg gacggcacgc cgagatacat ggcgctggca ccggtggcga cgatcagcgc
     2341 gtcgcagctg tattcgccgc tgtcgccgat cagccgcttc ggttcctcgt cgaggtgcac
     2401 ggtgtgcacc tggtcgaaga cgatctcggt ctcgaagcgc tccgcgtgct gttgcatgcg
     2461 ctgcatcagc accggcccgg tgagcccctc gacgtcgccg ggccaattgt cgacatcggg
     2521 ggtggtcatc agctgcccgc cggcctcgac gccggtgatg accatcgggc tgaggttggc
     2581 gcgcgcggcg taaaccgcgg ccgaatagcc ggccgggccg gatccgagaa tgatgacatt
     2641 gcggtgtttg atagcggaca ttgacaagtc tccgggatcc gggtgcgaaa aacggcgccc
     2701 atcctacagg ctgccgctgc ccgtgactat atggttattg ggatagcctt ttcaatggga
     2761 tgagcccgtc ttgccgagca tatttccgcc gccatcgccg atagactcgc cgtgacggtg
     2821 gctactcccg ccggtcggct cgacacgaat tcgcgctgcg agcgtgccgg ccaccagcca
     2881 gtgcgcgccg ccacggcggc ttctcggcag tcgggcgacg gaatccgata agaaaagcgg
     2941 ctttattctg gttcccgttc gagtggatca tgtaaaatgc caggatttac atgaggtttt
     3001 cgactcaaat tggtgcaagc aaaactcaag tcacgagcgg acgacgaaca aaaaggacgc
     3061 atgtttgccg gtcacgtcgg ccagcgcctg cgcgaggccg gtatgttatt gtcgttcgcg
     3121 ctggccggct acttcctgtt ggcgatgttc agctacgacg cgctcgaccc gtcctggtcg
     3181 catagcggca gcaacagcga gatcgccaac ttcggcggcg cggccggcgc ctgggtcgcc
     3241 gacctgttgt tctacctgtt cggctacctg gcctacctgt tgccgataat gctgatctat
     3301 aacggcatcc tgctggttcg cagccgtgat ctgagtgccg aggcccagta tcacatgctg
     3361 gtgattcgct ggagcgggtt cgtactgacg ctgatttcgg gcagcgcgct gtcgagcctg
     3421 catttcgtgg tggtgcccgg cagcatgcca ctcgacgcgg gcggtattct cggccagatc
     3481 accggcaatt atttttccca ggggctcggc ttcatcggcg ccacggtcat gcacctggcg
     3541 ttgtttctcg ccggcctgac gctgttcacc gggctgtcgt ggctggcgct ggtcgacaac
     3601 accggccgct gcacgctgct ggccatcgac ggcctgctgg atcgttacta cctgttacgc
     3661 gatcgcttcg agggcaagcg tcatcgcagt gagcgcgcac aggtatttca ggtgcagcaa
     3721 aagaagcagg aaacccgcaa gccgcccaag atcgaaccgg tagtggagaa aatagagccc
     3781 agcccgcgag tcgaaaagga gaagcagatt ccgttgttca gccccggcgg taacggcaag
     3841 ggatcgatac cgccgctggc gctgctcgac aagccgcggc cgcgcgttgc cgcctacagc
     3901 aagtcggccc tcgaagccat gtccaggcaa ctggagctga agctgctcga tttcggtatc
     3961 gagatccagg tcgaatcggt gcacccgggg cc
//
LOCUS       DATCZV010000466         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_90946, whole genome shotgun sequence.
ACCESSION   DATCZV010000466 DATCZV010000000
VERSION     DATCZV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90946"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VIW27_10495"
     CDS             <1..660
                     /locus_tag="VIW27_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HEY5740145.1"
                     /translation="RPGAAEIRKLPPSMLDARLLADYVGSGEAFDLWELCKNSGIPLR
                     KIAHLVAARSLGDVDKLPGYAVTRDVYQVIEDIYCCGATRREWQRVRTRHKPEYLEGR
                     MSRERLRELRAADPQAYRRHYARIYFDAWAAFWDDAFLERMIDQLDSRLIGSVPEYLL
                     NEKICFRAVSRWGLALRYMPERFRTLAICARAVEQDPLAIRFAPARLREELRRISGPA
                     R"
     gene            complement(837..1379)
                     /locus_tag="VIW27_10500"
     CDS             complement(837..1379)
                     /locus_tag="VIW27_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740146.1"
                     /translation="MKPALIAMLLAAAGPALPASDSPYEGQQLRQIKSLSDAEIDGLL
                     RGEGMGFARAAELNHYPGPAHVLELAAELQLSEQQLAQTRAIHERMNATARRLGAQLV
                     DGEKALDELFAQATITPQSLTRQLSIIGDIRARLRGVHLQAHLEQRALLTQHQVHRYD
                     ALRGYLDGGSGHAHTHGGHN"
     gene            1663..2472
                     /locus_tag="VIW27_10505"
     CDS             1663..2472
                     /locus_tag="VIW27_10505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5740147.1"
                     /translation="MADYASAWEKWFPTIEQGASTLTACMLDAAGLEAGQRVLDVATG
                     IGEPALSAAGRVGPAGRVTGIDLSSAMLEFARRRARDAGLDNAEFRVMDANALDLAAA
                     SYDAALCRWGLMFVDDLAQTLCAIHAALKPGARLAIGVWGSADEVPALSLAARVLHRE
                     LGLPPPPRGAGTAFALADGASLVAAMQAAGFEQVTRRAVNVHYDYASPRQYLAHRLEV
                     SNLLRATLDGIAEPRIAAAKAVLEQALEARRREDGSIGFDNRAYCVAATRS"
     gene            2581..2781
                     /locus_tag="VIW27_10510"
     CDS             2581..2781
                     /locus_tag="VIW27_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY5740148.1"
                     /translation="MQKVLIIVGLAILLIGLAWPWLGSLPFGRLPGDIVIRRDDFTFY
                     LPITTMIVVSLGLSLLIWILRR"
     gene            complement(2857..3135)
                     /locus_tag="VIW27_10515"
     CDS             complement(2857..3135)
                     /locus_tag="VIW27_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEY5740149.1"
                     /translation="MFKDYFQNLTGPSKNFHGVTASPAYQWRGNHASPLSKLIGRLRA
                     TLAGIDRRRRMEYENRRAIAHLESLSDEQLRDIGIARELIERSVRLGR"
BASE COUNT          532 a         1043 c         1110 g          645 t
ORIGIN      
        1 cgccccgggg ccgcggagat ccgcaagctg ccgccctcga tgctggacgc acgcctgctg
       61 gccgattacg tcggcagcgg cgaagctttc gatctgtggg agctatgcaa aaactcgggc
      121 atcccgctgc gcaagatcgc gcacctggtt gcggcgcgca gcctcggcga cgtcgacaag
      181 ctgccgggat acgcggtcac ccgcgacgta taccaggtaa tcgaggacat ctactgttgc
      241 ggcgccacgc ggcgcgaatg gcagcgggtg cggacccgcc acaagcccga atacctcgaa
      301 ggtcgtatgt cgcgcgagcg cctgagggaa ctgcgagccg ccgacccgca ggcataccgg
      361 cgccattatg cgcggatcta tttcgatgcc tgggcggctt tctgggacga tgcctttctg
      421 gagcgcatga tcgatcagct cgacagccgc ctgatcggca gcgtgcccga atacctgctc
      481 aacgaaaaaa tctgttttcg agcggtctcc aggtgggggc tggcgctgcg ctacatgccc
      541 gagcgctttc gcacgctggc aatttgcgcg cgcgccgtcg agcaggatcc gctggcgatc
      601 cgtttcgcgc cggccaggct gcgcgaggaa ttgcgccgca tttccggccc ggctcgctga
      661 ttcacggtga tcgagccgag cgggccgcag tcggctatag cggacagaaa attgccgaca
      721 atgccgcggc cccgccaccc atgatcagtc tggtgcgcca gccggcaggc gcaccgctgg
      781 caacaagcaa ccggtaattc acccatcgcc gcgggcattt gccggctgcg gtggtgtcag
      841 ttgtgtcccc catgggtatg cgcatgcccg ctaccgccgt cgaggtagcc gcgcagtgca
      901 tcgtagcggt gcacctggtg ctgggtcagc aacgcgcgtt gctcgaggtg ggcctgcagg
      961 tggacaccgc gcaagcgggc gcgaatatcg ccgatgatcg atagctgtcg tgtcaggctc
     1021 tgcggtgtga ttgtcgcctg ggcgaacagc tcgtcgagcg ctttttcgcc gtcgacaagc
     1081 tgcgccccca ggcgcctggc cgtggcgttc atgcgctcgt ggattgcgcg ggtctgcgcg
     1141 agctgctgct cgctcagctg cagttcggcg gccagttcga gcacgtgagc ggggccgggg
     1201 tagtgattga gttcggcggc gcgcgcgaaa cccatgcctt cgccgcgcaa caggccgtcg
     1261 atctcggcgt cgctgaggga tttgatctgg cgtagctgct ggccctcgta gggcgagtcc
     1321 gacgctggca gagcggggcc cgcggcggcc agcagcatcg cgatcaaagc gggtttcata
     1381 ctcgtttctc cggtggtttg cgagcgcggc atgccgttcc aggcaccgcc gcgagctgca
     1441 gattgcgcaa cttaatcgag atctgtcggc cttgcaactc gtgcggcggg ccacgcaggc
     1501 tgggcctgcc attgccgacg ggcatattcc gtggccgccg ttggtgatcg ccgcattcgg
     1561 ccgcaaacgg ggcgaggatg ttatcatcgg cgcgactggc tgccgacgag ttcgcgggcc
     1621 gctgtgcccg cgcgcaggtt gcgacagcag aggggtgatc gaatggcgga ttacgcatcg
     1681 gcctgggaga agtggtttcc gacaatcgag cagggtgcga gcactctgac cgcatgcatg
     1741 ctcgacgctg ccggtctcga agcgggtcag cgcgtgctcg acgtcgcgac cggtatcggc
     1801 gagccggcgc tatcggcggc cggtcgcgtc ggtcccgccg gacgggtgac cggtatcgac
     1861 ctgtcctcgg cgatgctcga gttcgccagg cggcgcgccc gcgatgccgg gctcgacaac
     1921 gccgagtttc gagtcatgga cgccaatgcc ctcgatctcg ctgctgcctc gtacgatgcc
     1981 gcgctgtgcc gctggggcct gatgttcgtc gacgacctcg cgcagactct gtgcgcgatt
     2041 cacgccgcgt tgaaaccggg agcgcgcctc gcgatcggtg tctgggggag cgcggacgag
     2101 gtaccggcgt tgagcctggc ggcgcgcgtg ctgcaccgcg agctgggctt gccgccgccg
     2161 ccgcggggcg cgggcaccgc ttttgcgctg gccgatgggg cgtcgctggt cgctgccatg
     2221 caggcggccg gatttgagca ggtgacccgg cgcgccgtca acgtgcacta cgactatgcc
     2281 tcgccgcgcc aatacctcgc gcaccgcctc gaggtttcca acctgctgcg cgcaacgctg
     2341 gacggcatcg ccgaaccgcg gatagcggct gccaaggcgg tactcgaaca ggcgctcgag
     2401 gcccggcggc gcgaggacgg cagtatcggt ttcgacaacc gcgcctactg cgtcgcggcg
     2461 actcgaagct gagttccgcg gctagccctg tcaccggacg gagacggttc gcccggcggg
     2521 attttccggg cgccggccga atcgcgctat gataatcgat cgtgcctcgc gggtgccgcc
     2581 gtgcaaaaag tactaatcat cgtcgggctt gcgatcctgt tgatcgggct cgcctggccg
     2641 tggctcggca gcctgccgtt cggccgtctg cccggggata tcgtcatccg ccgcgacgat
     2701 ttcaccttct atctgccgat tacgacgatg atcgtcgtca gcctgggtct gtcgttgctg
     2761 atctggatac tgcgccgcta gtccgcggtc gaaaaattta agccgatcga ataccgcgta
     2821 tagttgcgcc caccctactg cagctgccgg gagtgcctag cgtcccagcc gcacgctgcg
     2881 ctcgatcagt tcgcgtgcga tgccgatatc gcgcagttgc tcgtcgctca gcgattcgag
     2941 gtgcgcaatc gccctgcggt tttcgtactc catgcggcgt ctgcggtcga ttccggcgag
     3001 cgtcgcgcgc agccggccaa taagtttgct gagcggcgac gcatggttgc cgcgccactg
     3061 gtaagccggg ctcgcggtta ccccatgaaa gttttttgag gggccggtca gattttgaaa
     3121 gtaatcttta aacattgtag aattcctcaa tttataaatt ccgcagtgat tgcctaaatt
     3181 tgttttgata gtggcaatta ttcaactata tatgtcagtt ttcaaacgag tattcttttg
     3241 aataaattta gtaatactaa aattaaaaca cgccgcctgc cgccgttgaa tgcgttgcgc
     3301 gcgttcgagg ccgccgggcg gctcgaaagc
//
LOCUS       DATCZV010000467         5398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_569529, whole genome shotgun sequence.
ACCESSION   DATCZV010000467 DATCZV010000000
VERSION     DATCZV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569529"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VIW27_10520"
     CDS             complement(<1..584)
                     /locus_tag="VIW27_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5740150.1"
                     /translation="MSDTTRLRAVAAQGAPAQDRKQLQRFDWLQRALEIFVAEGIDAV
                     RITRLADDLGVTRGSFYWHFANRDDLIDALVRFWQDKNTAAIEQLVVQARSLADGIFR
                     YFETCLDAERFDARLDLAIREWARRSTRIRELLDREDSARVEALRQFFTRFEYPMPEA
                     LIRARVLYYSQIGFYALETREPLTTRLSYTEAYF"
     gene            652..1008
                     /locus_tag="VIW27_10525"
     CDS             652..1008
                     /locus_tag="VIW27_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009813760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutQ"
                     /protein_id="HEY5740151.1"
                     /translation="MTAVKAAPRLVRFGDLEFVPRFEYGEMAEVAGICGTADGTELGA
                     GWGRLRNARIPWTIRYDEVLTVFEGLLRVHTGGEVHELHPRDCLWLPKGTELVYEADS
                     ALVHFAIHPADWQQPA"
     gene            1005..2330
                     /locus_tag="VIW27_10530"
     CDS             1005..2330
                     /locus_tag="VIW27_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5740152.1"
                     /translation="MSARRIGHLPLNDNGNGWSRMLAPRAPQPALAGKRRADWVVVGA
                     GYAGLAAARRLAENRPSAEIVLIEAGEAGENASGRNSGFAIDLPHVISSTQNELEGSH
                     RYMALARAAIAYHETQIERHGIDCDWTQPGKYQTASSDAGVRDMLEPFARELDALGES
                     YTWIDKPDLDRRLGTSHFKAAVYTPGCRLLNPAALVRGLADSLPQNVTLYENTPATGV
                     DTDGPVTLTTRDGEVVADGMILGVNAFAQHFGYYPRQLLPFAANASLSRRLTAAERDA
                     LGCEDNWGVTPANAFASITMRFTNDHRILIRNNIYYNPKMRETSAYQAQIARRHKRLF
                     DERFPMLPEVGMQYTWTGYLCLSQNGAPGFGRLADNVYCAVCQNAMGVTKGTAGGMLA
                     ADMACGVDNELIGFMQSLGEPSKLPFEPLLGIGVRARLAWELWRGRHEA"
     gene            2491..3408
                     /locus_tag="VIW27_10535"
     CDS             2491..3408
                     /locus_tag="VIW27_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5740153.1"
                     /translation="MADTLAVQIEAPRPSYLVGVVLVLLAGAFWSSMGVGIRLIETAN
                     VWQILLYRSMALALFLFAIIAWRSGYRPLQRIRKAGLAGVIGGAGLVMAFAGGIYAIQ
                     TTSVANAMFLFASAPLLAAFLGWVLLRESVRRATWVAMLVGMAGVAVMVANGISAGRA
                     AGNLSALLSACGFAVFTIALRWGRLEDMMPAVFLAGVFASLTAALACAIGGHAYTIPA
                     NDVAIALALGVFQVGVGLTIFTIGSKVVPAAELALLSMTEVLLGPLWVWLFLGETADP
                     ETLLGGAILMLAIAGNALSGLRRKPVPVL"
     gene            3408..4898
                     /locus_tag="VIW27_10540"
     CDS             3408..4898
                     /locus_tag="VIW27_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014290276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEY5740154.1"
                     /translation="MAANHLFDGLLGGRESAPGTLLRVPGGESWSYADTVELSGRLAN
                     LLVELGVTPGDRVAVQVQKSPQAIALYLATVRAGAVFLPLNTAYTRNEVGYFLDDAEP
                     ALLVCDGARAAEFDGIDAQLMTLDGDGGGSLASAATTHAAAFDNVERGPGELAAILYT
                     SGTTGLSKGAMLSHDNLLSNSVTLVDYWRFTEDDVLLHALPIFHTHGLFVATNVTLIC
                     GGSMIFLARFDVDTVIDELPRATAMMGVPTFYTRLLADDRFERELVAHMRLFVSGSAP
                     MLAETHAQFEQRTGQRILERYGMTETNMNTSNPYDGERRAGSVGFALPGVEVKVTDPE
                     SGESLADGETGMIEVRGPNVFLGYWRKPEKTAEELRADGFFITGDMGVIAEDGYLHIV
                     GREKDLIISGGYNIYPKQIETEIDALDGVVESAVFGIADADFGEAVAAAVVLEPGAAL
                     DRDGLLQQLAPQLARFKLPRKIVFVDELPRNAMGKVQKNLLRDRYG"
     gene            4891..>5398
                     /locus_tag="VIW27_10545"
     CDS             4891..>5398
                     /locus_tag="VIW27_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY5740155.1"
                     /translation="MDELIARSARDVVAMLRAGEIGIADALDALQSRIAAVDGEINAL
                     PTLCFERAREQAGKRDYRNTLLGGIPVAIKDLEDVAGVRTTYGSRVYEHHVPAQSDLV
                     VEHIEARGGIVYAKSNTPEFGSGANTFNDVFGATRNPHDPTLSAGGSSGGAAAALASG
                     TAWLAQGSD"
BASE COUNT          893 a         1724 c         1804 g          977 t
ORIGIN      
        1 tcgaagtaag cctcggtata tgacagccgg gtggtcaacg gttcgcgggt ttccagcgcg
       61 taaaagccga tttgcgagta gtagaggacc cgggcgcgga tcagcgcctc tggcatcggg
      121 tattcgaaac gcgtaaaaaa ctgccgcagc gcctcgacgc gcgcgctgtc ctcgcggtcg
      181 agcagctcgc gaatccgggt cgagcgccgc gcccattcgc gaatcgccag atcgaggcgc
      241 gcatcgaagc gctcggcatc caggcaggtc tcgaagtagc gaaagatgcc gtcggcgagg
      301 cttcgcgcct ggaccaccag ttgctcgatg gcagcggtgt ttttgtcctg ccagaagcgg
      361 accagtgcat cgatcaggtc gtcgcggttg gcgaagtgcc agtagaagct gccgcgggtg
      421 acgccgaggt cgtcggcgag acgcgtgatg cgcaccgcgt cgataccctc ggcgacgaaa
      481 atttccagcg cgcgctgcaa ccagtcgaag cgctgcagct gtttgcgatc ctgggcggga
      541 gcgccctgtg cggcgaccgc cctgagccgg gtggtatcgg acatggttga tccatacagt
      601 tatgtatgtt tgaatataca gatctgtatt ttttatgcaa ggttgatttc gatgactgct
      661 gtcaaagccg cgccgcgcct ggtgcgtttc ggcgacctcg agttcgtgcc gcgcttcgag
      721 tacggtgaaa tggccgaggt cgccggtata tgcggtaccg cggacggtac cgagctcggc
      781 gccggctggg gccggctgcg caatgcccgc attccgtgga cgatccgtta cgatgaagtg
      841 ttgacggttt tcgaaggtct gctcagggtg cacactggcg gcgaggttca cgaactgcat
      901 ccccgcgatt gtctgtggct gccgaagggc accgagctgg tttacgaggc cgactcggcg
      961 ctggtgcatt tcgcgattca cccggccgac tggcagcagc cggcatgagc gcgcggcgca
     1021 tcgggcacct gccgctcaac gacaacggca acggctggag ccgcatgttg gcgccacgcg
     1081 caccgcagcc ggcgcttgcc ggcaagcgcc gggccgactg ggttgtggtt ggtgcgggtt
     1141 acgccgggct ggccgcggcg cggcggctgg cggaaaaccg gccgtccgcg gagatcgtgc
     1201 tgatcgaggc cggtgaggcc ggcgaaaatg cctccgggcg caactcgggt ttcgccatcg
     1261 acctgccgca cgtgatcagc agtacgcaga atgaattgga gggctctcac cgctacatgg
     1321 cgttggcacg cgcggcgatc gcctaccacg aaacccagat cgaacgccat ggtatcgatt
     1381 gcgactggac gcagcccggc aagtaccaga ccgcgagttc cgatgccggc gtacgcgaca
     1441 tgctcgagcc gttcgcgcgc gaactcgacg cgctcggcga aagctatacc tggatcgaca
     1501 agccggatct cgaccgccgg ctcggtacct cgcatttcaa ggccgccgtg tacacgcccg
     1561 gttgtcgact gctgaacccg gcggcgctgg tgcgcgggct cgccgacagc ctgccgcaaa
     1621 acgtcaccct atacgagaac acgccggcca ccggtgtcga taccgacggt ccggtaacgc
     1681 tgaccacgcg cgacggcgaa gtcgtcgccg acggcatgat cctcggcgtc aacgccttcg
     1741 cgcagcattt cggctactac ccgcggcaac tgctgccgtt cgctgccaac gcgtcactca
     1801 gccgccggct gacggcggcc gaacgcgacg cgctcggctg tgaggacaac tggggtgtga
     1861 ccccggcgaa tgcgttcgcg tcgatcacca tgcggtttac caacgaccac cgcatcctga
     1921 ttcgcaacaa tatctactac aacccgaaaa tgcgcgagac cagcgcctac caggcgcaga
     1981 tcgcgcgccg ccacaagcgc ctgttcgatg agcgtttccc gatgctgccc gaggtcggca
     2041 tgcaatacac ctggaccggg tacctgtgcc tgtcgcaaaa cggtgcaccc ggtttcggca
     2101 ggctcgccga taacgtgtat tgcgcggtct gccagaacgc aatgggcgtc accaagggca
     2161 ccgccggcgg catgctcgcc gccgatatgg cctgcggcgt ggataacgag ctgatcggtt
     2221 tcatgcaaag tctcggtgaa ccgagcaagc tgccgttcga acccttgctc ggtatcggcg
     2281 tgcgcgcgcg tctcgcctgg gagttgtggc ggggtcgcca cgaggcctga tcgattgctc
     2341 gctgcgggcg gtcgcgccgg tcgcgccgcc gactgcggtg tccgccgccg cagccgggcg
     2401 ggtgccggtc cggcggcggg taatcagggc tttgcattcg cgctgccttc gattaatctc
     2461 gccatcaatc cctggcggcg aaaatcgttg atggcagata ccctcgcagt gcaaatcgaa
     2521 gccccgcggc cgagctacct ggtcggtgtc gtgctggtgt tgctcgccgg cgccttctgg
     2581 tcctcgatgg gcgtcggcat acgcctgatc gagaccgcca acgtctggca gattctgctc
     2641 taccggtcga tggcgctggc cctgttcctg tttgcgatca tcgcctggcg ttccggctac
     2701 cgtccgttgc agcgaattcg caaggctggc ctggccggcg tgatcggcgg cgccgggctg
     2761 gtaatggcat ttgccggtgg catctacgcg atccagacga ccagcgtcgc caatgcgatg
     2821 ttcctgttcg cgtccgcgcc gttgttggcc gcgtttctcg gctgggtgtt gctgcgcgag
     2881 tcggtgcgcc gcgcgacctg ggtcgcgatg ctggtgggaa tggccggggt cgcggtgatg
     2941 gtcgccaacg gcatatcggc cgggcgcgcg gcgggtaacc tcagcgcgct gctgtcggcc
     3001 tgcggattcg cggtgttcac catcgcgctg cgctggggca ggctggaaga catgatgccg
     3061 gcggtattcc tggccggggt attcgcgtcg ctgaccgcgg cactggcctg cgcaattggc
     3121 ggtcacgctt atacgatacc ggccaacgat gtcgccatcg cgctggcact gggcgttttt
     3181 caggtcggtg tcggcctgac catattcacc atcggctcga aggtggtgcc ggcggccgaa
     3241 ctcgcgttgc tgtcgatgac cgaggtgctg ctggggccgc tgtgggtgtg gctgtttctc
     3301 ggcgagaccg ccgatcccga aaccctgctc ggcggtgcga tcctgatgct ggcgatcgcc
     3361 ggtaacgcac tgtcgggcct gcgccgcaag ccggtgccgg tactgtaatg gcggctaatc
     3421 acctgttcga cggcttgctc ggcgggcgcg aaagcgctcc cgggactctg ctgcgggtac
     3481 ccggcggtga gtcctggagc tacgccgaca cggtcgagct gagtggacgg ctcgccaacc
     3541 tgctcgtcga gctcggggtg acgccgggcg accgggtagc ggtgcaggtg cagaaatcgc
     3601 cgcaggcgat cgcgctctat ctcgccacgg tgcgtgccgg cgcggtgttc ctgccgctca
     3661 acacggcgta cacgcgtaac gaggtcggtt actttctcga cgatgccgaa ccggcgctgt
     3721 tggtttgcga tggtgcgcgc gcggccgaat tcgacggcat cgacgcgcaa ctgatgacgc
     3781 tcgacggcga tggcggcggc agcctcgcga gcgccgccac aacgcacgcg gcggcgttcg
     3841 acaacgtcga gcgcggcccc ggcgaactgg ccgcgatcct ctacacctcg ggcaccacgg
     3901 gcctgtccaa gggtgcgatg ctgagccacg ataacctgct gtccaattcc gtgacactgg
     3961 tcgattactg gcgcttcacc gaggatgacg tgttgctgca tgcgctgccg atctttcata
     4021 cccacggcct gttcgtggcc accaatgtga cgctgatctg cggcggctcg atgatcttcc
     4081 tggcacgctt cgacgtcgac accgtcatcg acgagctacc gcgggcgacg gcgatgatgg
     4141 gcgtgccgac cttttacacg cgcctgctcg ccgacgatcg cttcgagcgc gagttggtcg
     4201 cccacatgcg cctgttcgtc tccggcagtg cgccgatgct ggccgagacc cacgcgcagt
     4261 tcgaacagcg caccgggcag cgcattctcg agcgctacgg catgaccgag accaacatga
     4321 atacctccaa tccctacgac ggcgagcgcc gcgccggcag cgtcggcttc gcgttgcccg
     4381 gtgtcgaggt caaggtcacc gatcccgaaa gcggagaaag tcttgccgat ggcgagaccg
     4441 gcatgatcga ggtgcgcggg cccaatgtat ttctgggtta ctggcgcaag cccgaaaaaa
     4501 ccgccgagga attgcgcgcc gacggtttct ttatcaccgg cgacatgggc gtgatcgctg
     4561 aagacggtta cctgcacatc gtcggccgtg aaaaagacct gatcatttcg ggtggctaca
     4621 acatctatcc gaaacagatc gagaccgaga tcgacgcgct cgacggcgtg gtcgaatcgg
     4681 cggtatttgg cattgccgac gccgacttcg gcgaggcggt cgccgccgcc gtggtcctcg
     4741 agcccggcgc cgcgctcgac cgcgacggcc tgctgcagca actggcgccg caactcgcgc
     4801 gcttcaagct gccgcgcaag atcgtgttcg tcgacgagct gccgcgcaac gcgatgggca
     4861 aggtacaaaa gaacctgctg cgcgatcgtt atggatgaat tgatcgcacg cagcgcccgc
     4921 gatgtcgtgg ccatgctgcg cgcgggcgaa atcggcatcg ccgatgcgct cgacgcgttg
     4981 cagtcgcgga ttgccgcggt ggacggggaa atcaacgcgc tgccgaccct gtgtttcgag
     5041 cgcgcccgcg aacaggccgg caaacgggat tatcgcaata cgctgctcgg cgggattccg
     5101 gtcgcgatca aggatctcga agacgtcgcc ggggtgcgca caacctacgg ttcgcgggtc
     5161 tacgagcacc acgtgccagc gcaatccgat cttgtcgtcg agcatatcga ggcacgcggc
     5221 ggcatcgtct atgccaagtc gaatactccc gagttcggct ccggcgccaa caccttcaac
     5281 gatgtcttcg gcgcgacgcg caacccccat gacccgacgc tgagcgccgg gggatcctcg
     5341 ggcggtgccg ccgccgcgct cgccagcggc accgcctggc tggcacaggg ctcggatc
//
LOCUS       DATCZV010000468        15836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_674053, whole genome shotgun sequence.
ACCESSION   DATCZV010000468 DATCZV010000000
VERSION     DATCZV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15836
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674053"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VIW27_10550"
     CDS             <1..505
                     /locus_tag="VIW27_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5740156.1"
                     /translation="NWKAYGDNSCEGYHVGMVHGALGSSMERESVAIAAHENGQFVGF
                     DVTYGKGHDQSRAGRGFWIYKFPGLLLHFSEYAFNAETVLPLSAGAIELRRWFWSNAA
                     ACREHGIEPASIRPNSEQVMDEDIAICEAVQRNLAAGVYPGGRLCARQEPGTIYFQRL
                     VRAALGI"
     gene            complement(494..1645)
                     /locus_tag="VIW27_10555"
     CDS             complement(494..1645)
                     /locus_tag="VIW27_10555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740157.1"
                     /translation="MNSPYLAVAAAMLVTFVLGSVHAFSVFIAPLEALLRLPRAEISL
                     IYSLALVALTVSVLVGYRIYHRLAPASLVLLTCLAAAAGTALAALVPAWWALCVGYSL
                     VFGLSNGIGYGYCLQLAGRVMPRRRGFAMGAVTATYAVGSVVFAKVFAWRIAAAGVDA
                     ALFAVAAALLAGGAAAALMLRASGASYGLPGAPNVADDSTAGSGVAIFWLAYLGAVFA
                     GLMAIGHAAGIALSRDATPQLATGAAVAAGIGSALGGFVAGWMIGRWGSGRLLVGLPL
                     LSAVALLAMAVHEDAFGTVMLLSLVGFAYGSLIAVYPVAIAERYRTQGPRVYGRVFTA
                     WGCAGLAGPWSAGWIFDWRGGYETALLAAAGIALLSSLCARAIRPASNA"
     gene            complement(1638..2567)
                     /locus_tag="VIW27_10560"
     CDS             complement(1638..2567)
                     /locus_tag="VIW27_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5740158.1"
                     /translation="MPSANPSLFETPAHRDRPALGVATFTGNLLVMALLSAAVRELVQ
                     RDYPLAEVLLFRYLFASLLFWVILGCGAGLAALATRRPLDHAIRSVSGIASLALLYFA
                     ITRIPLADATALAYAAPIFITVLAIFLLGEKIGLRRWLAVIAGFAGVLLIARPVAGGW
                     DIGVAAAAGSAFTGALVAIWLRKLSRSEHPAAIGIYYNNLGLLLCLGWVLASGWLTPR
                     GADLGLFVVFGLGCGVQQWLLTISFRYAEASLLAPYEYLAMVFAAIVGYLFWGEVPVL
                     STWIGAAVIAGSGIFIFARRQRRRQAAEPVIIE"
     gene            complement(2577..3488)
                     /locus_tag="VIW27_10565"
     CDS             complement(2577..3488)
                     /locus_tag="VIW27_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5740159.1"
                     /translation="MSRYQSNNLLGIGLMLLAMLLFEVMDAVAKLLVSADMSALQVIA
                     VYSWFITVMIPSILLLRGELGERVTRKPWHHLLRGMVGFVAPLSFFSALQSLPLADAT
                     VVFFSGAFILTAASALLLGERVGVHRWSAVVIGLGGVVIAVNPGGGGPLPAYLLVLCA
                     ATVYALIFITGRQLSRRDSVISLAFSLHLGMGIVASALLPRFWVPLDALVIVQLLLMA
                     LIALVAHYVFVAAFARAEVSVLAPFEYTALVWATIIGYLVWRDIPPYEVWLGAAIIIG
                     CGLYVIHRESLQRKSPALAPEHCDPLP"
     gene            complement(3553..5430)
                     /locus_tag="VIW27_10570"
     CDS             complement(3553..5430)
                     /locus_tag="VIW27_10570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5740160.1"
                     /translation="MNNPKLTFDVEPALEAELARFRDQQALLDRTELIAHIGHYEWSY
                     QLECLLACSEEYARIHDMSVKQVLRAHGSLAGFMRQVHPDDVESFEQASETLRREHAV
                     DRVYRLRCASGEIRHIRECAVEVIDDQGVAIGAFGILQDISDTVRYERDLEYRDELAR
                     QSEALTDIGHFIYDEENQRYVYVSDGLARIYGTSAEAYMRDVSSSEDDLADILPEDRE
                     RVAEAYRAYIDDGEDCVLEFRIRRDDGSVRWLRELTCAKKKRDGRVIQTLGVIQDITP
                     QKIIEQELLYKDELASQAEAITEIGHFIFDQVSETYAYLSPGFARIHGLSVEEYKSTV
                     NSRNDDIDDLHAEDYERVLEAYDQHRRTGEDLNIEYRIYRSDGAIRWIREQVTSVPGP
                     GGEPRESIGVIQDITEQKNTEKHLREARDSLEQMVKNRTRKLAETVGQLQAEIKEREK
                     ISAELAFLANHDALTGLPSLRLCKDRLDHSLAEARRKRQLSSVMFLDLDGFKEVNDAH
                     GHEFGDRVLKTISERITEEIRETDTVARIGGDEFVIISSGLPDRSIAERIASSLIRHI
                     SRPLRIEADEVTVSASIGIAFYPGDGSTAEELIRSADQAMYRVKHRGKNNFGFAGGHE
                     S"
     gene            5623..7020
                     /locus_tag="VIW27_10575"
     CDS             5623..7020
                     /locus_tag="VIW27_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5740161.1"
                     /translation="MNRPATRVSQREDVIRQLRELLGERTSTNESLLEQHGRGESWHP
                     VQAPDVVCFPQHNDEVAAIVRLCAESATPVIAYGTGTSLEGQVQALQGGVCIDLSRMN
                     RIIEVNTEDMDCRVQAGVTRRELNHYLRDSGLFFAIDPGADTSIGGMTATRASGTNAV
                     RYGTMRENVLGLTVVTANGEIIHTGTRARKSAAGYDLTRLLVGSEGTLGVITEVCLRL
                     HGRAEAISAAVVNFPDVRSAAEATILTIQSGIPMARIEILDSAYVAAINAYSDTDYPE
                     LDTLFLEFHGTRAGVAEQVRMFEEISREFGASGFQWAEREEERNRLWRARHDAYYAGL
                     AARPGYRGYVTDVCVPISRLADCIAETQADIAESGLFAPIIGHVGDGNFHSTIFIDPN
                     DEAMLAKALELDQRMVRRAQAMGGTCTGEHGIGIGKLKHMRNEHGDGAIVVMQAIKAA
                     LDPQNIMNPGKVVDC"
     gene            complement(7455..8045)
                     /locus_tag="VIW27_10580"
     CDS             complement(7455..8045)
                     /locus_tag="VIW27_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEY5740162.1"
                     /translation="MNHFAHLVLAQPTLESTVGNLLGDFARGVDVDRLPHAVRAGLHN
                     HRAVDRFTDAHPRVQAMKRAFPSSRRRFAGIALDIYFDHLLINHWRQFEQRDLDRLIA
                     DFYRRMQKGRGLMPGENMRRVTRRMIEYDWFGSYRDLDAIAESLDRVAGRIRFENRFA
                     GAIEDLQRHHDLICEGFFEFYPALQRHVEQLALESD"
     gene            8147..8923
                     /locus_tag="VIW27_10585"
     CDS             8147..8923
                     /locus_tag="VIW27_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5740163.1"
                     /translation="MPISVVDTEKQSSGADPYREQRADLAAAFRMAARLGMHEGVANH
                     FSLAVSDDGKRFLMNPFGRHFASVRAIELLLLDADDDNTMERADRPDPTAWAIHGAMH
                     RNLPQARCVLHVHSKYATALASVEVDGLPPIDQNTMRFFNRVSLDDGFSGMGLGEEAE
                     RLTTTLGDNRVLLMGNHGVMVVGENVARAFDELYYFERACETYVTALATGKPLRIASD
                     AVAETTARQWEDYVQRVGIADTHLRELRAILDREEPDYRL"
     gene            8994..12377
                     /locus_tag="VIW27_10590"
     CDS             8994..12377
                     /locus_tag="VIW27_10590"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5740164.1"
                     /translation="MSASVSAWLQELGLEQYAELFEANDIDWELLADIDQQTLREIGV
                     QSVGHRIRILKAIGKLQAPAALAPAAAAAPGEAAADPDITAWQRRPGERKPVTMLFAD
                     LVGSTALTEELDAEEAHDLVYLATQRMCRAVESNNGTVCRFMGDGIMAMFGAPLASER
                     HALEACRAALAMQAGIAEYAAQLDGDHADRVKIRVGLHSGEVVVLEVGDDPQNPEYDA
                     SGPTVPLAARMEQSANPGNILISAETRMLAGAAIATRDHPPVAVKGFSEAVEVFELER
                     ILSAAESARVAIEKPIIGRRSELAQFRGLLESCVEDNSGQCLHVRGEAGIGKTRLVGE
                     MTRLAQSRDYLTHKALVLDFGTGRGQGAIPSLLKSLLGTDENAAGPECAAALARAISD
                     GLLDEQNRVFMNDLLDLEQPLELKTLYDAMDVETRNEGKRVALAELINRLAARSPLAI
                     VFEDLHWADAATLDYLASIAAAVVDSAVLLVFTSRVEGDPVDEKWRGAAGGIPIVTWD
                     LRPLRRAECLQIVSAFTNTDVAHIERCIERADGNPLFLEQLLLSSPAGGGRIPDSIKS
                     LVLARIDQLPVADKRALQAAAILGQRFDLDSLLFLVDDPGYDCGSLIERQLIKPEGPY
                     YLFVHALIQEGAYASLLKAQRAALHAKAAARYRERDAELHAVHLDQAGDSGAAAAYLA
                     AGREYLAAYRPERALEFARRGIEIAPAATRFALKCLEGELLRAIGATGESIEVFRAAM
                     ELAGDDAERCRALVGLAEGLAIIEAHDELERVLAAAETLARHLDYWLELAVIHQLRGG
                     MFFFQGEHEQCLSACNFSLECARKTDTPEVVARALSGLADATYCRGRFHSANRYYTEC
                     IDFSRQHGLGRVLAANLSMRGFTFRYRNQPEAALRDYREALELAARTRHLRAESVACY
                     AGLYLAEMGEHEEGEALLRRGLAISTSLGSSLLQSAALIALSRLIMNIGERAEALSLA
                     RQAIEILGASESGMAFRGPSALGALALATEDDDERRAALAEGKALLSSSSMGHNHFNF
                     CEDGIDTCLQIGDWDGVDWFTSHLRDYTRDEPLPFSEFIVARATALAEFGRGERSESL
                     LARLEELSEQAGSIKLFFQRQAVDQALEQLRAATAAKPS"
     gene            complement(12453..13532)
                     /gene="corA"
                     /locus_tag="VIW27_10595"
     CDS             complement(12453..13532)
                     /gene="corA"
                     /locus_tag="VIW27_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY5740165.1"
                     /translation="MAYFSKRYHPPGTSPGTLQQVEKQPGSSLRMLVVDYTASEFTER
                     ELATAEDSREFLERDTLTWIHVQGDAEPETMRQLGAVFGLHSLALEDVINSGQRPKID
                     DYDEQMFITLTHAARDPESGDVSLEQLSVFAGRNFLISFHPGTSDPFAPVRRRLRNNA
                     GRIRGRGIDYLLYSLLDLVVDEGFPVLESLGEELEQLEDELLEKPTRDLLPRLHRLKR
                     TLLIMRRMLWPQREVLSKLLRDESGIIGDDSQVYYRDCYDHTIQIMELIETYRDVITG
                     LLDIYLSSVSFRLNEIMRVLTVIATIFIPLTFIVGVYGMNFQNSDSPWAMPELRWYFG
                     YPLVWLLMIGVLVGMLVYFRRRKWF"
     gene            complement(13701..14648)
                     /locus_tag="VIW27_10600"
     CDS             complement(13701..14648)
                     /locus_tag="VIW27_10600"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEY5740166.1"
                     /translation="MKLPFERARGLACTLFIALFLGGCATSPQTRLLIDEPPPIPPRV
                     ELTEVPFFPQQEYHCGPAALAAIINYHGGAVIPDQIAEQIYVPELKGSLQVEVVAAAR
                     QYDLLPVVLDGRMESLLHELDAGNPVFVLQNLAIDLLPVWHYEIVIGYDLDDREMILR
                     SGTSRRTTRSFATFEKTWQRAGHWALVLVPPERIPVTASADVFLDAAIDMEQIGRTVT
                     ARKAYTAAAARWPDNLLAHSGLGNTAYALGDYAAAEAAYRAAIERAPGEAAIWNNLAY
                     ALAAQGRRTASLDAIRRAIEIEPDNANFVDSLSELSAAR"
     gene            complement(14657..15010)
                     /locus_tag="VIW27_10605"
     CDS             complement(14657..15010)
                     /locus_tag="VIW27_10605"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY5740167.1"
                     /translation="MLLKSIFRCLLIGIAALTVSIPAQAAMVGTAQLQAGSAAIELAA
                     LGEQRDWIREQLVVGGVSESKAAARVASMTDAQVQEVYQRIDAAPAGGADALLVVLIV
                     LIVLEVMGYTDFIKD"
     gene            complement(15074..15412)
                     /locus_tag="VIW27_10610"
     CDS             complement(15074..15412)
                     /locus_tag="VIW27_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEY5740168.1"
                     /translation="METIDWSDFERVELRVGTIIEAEEFPEARKPAYKLKIDFGDEIG
                     VRKSSAQITDLYSLDDLVGRQIVGVVNFSPKQIGPVRSECLVTGFNRDDGAVVLATPD
                     SRVPNGARLA"
     gene            complement(15422..>15836)
                     /locus_tag="VIW27_10615"
     CDS             complement(15422..>15836)
                     /locus_tag="VIW27_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5740169.1"
                     /translation="CKDGLDIVIAIQNEREWRRFCAGLLGDESLADDERLHNNKVRVD
                     NRAFVDELLGRAFADLDQAAAVERLRDADIAFGRLNDIAGLARHPQLRRERIATPTGE
                     ARVAAHAALRSNARERLRRVPGLGEHSDKIRKEFS"
BASE COUNT         2971 a         5199 c         5047 g         2619 t
ORIGIN      
        1 caactggaag gcctacggcg acaattcctg cgagggttac catgtcggca tggtacacgg
       61 cgcgctgggc tcgtcgatgg agcgcgaatc ggtggcaatc gcggcccacg aaaacggcca
      121 gttcgtcggc ttcgacgtga cctacggcaa gggccatgac cagagccgcg ccggccgcgg
      181 tttctggatc tacaagttcc cggggctgct gctgcacttt tccgagtacg ccttcaacgc
      241 cgaaaccgtg ctgccgctgt cagctggtgc catcgagctg cgccgctggt tctggtcgaa
      301 cgcggcggcc tgtcgcgagc acggcatcga gccggcgtcg atccggccga actccgagca
      361 ggtcatggac gaggacatcg cgatctgcga ggcggtgcag cgcaatctcg ccgccggcgt
      421 ctatcccggc ggccggctgt gcgcgcggca ggagcccggg acgatttatt tccagcgact
      481 ggtgcgcgcc gcactaggca tttgacgccg gccgaatagc cctcgcgcac agcgacgaca
      541 gcagcgcgat gccggcggcc gccagcaagg cggtctcgta accgccgcgc cagtcgaaga
      601 tccaccccgc gctccaggga ccggcgagcc cggcgcaacc ccaggcggtg aatacccggc
      661 cgtagacccg cgggccctgc gtccggtagc gctcggcgat cgccaccgga tagaccgcaa
      721 tcagcgagcc ataggcgaag ccgaccagcg acaacagcat gaccgtgccg aacgcatcct
      781 cgtggaccgc catcgccagc agcgcgaccg ccgacaacag cggcaggccg accagcagcc
      841 gcccgctacc ccagcgcccg atcatccagc cggcgacgaa accgcccagc gcactgccga
      901 taccggcggc caccgcggcc ccggttgcca gttgcggcgt cgcgtcgcgg gacagggcta
      961 tgcccgccgc gtggccgatc gccatcagcc cggcgaacac cgcgcccagg taagccagcc
     1021 agaagatcgc gaccccggaa ccggcggtgg aatcgtccgc cacattcggc gccccgggaa
     1081 ggccgtagct cgcccccgaa gcgcgcagca tcagcgccgc ggcggcgccg ccggccagca
     1141 gcgcggccgc caccgcgaac agcgccgcat cgacgccggc ggcggcgatc cgccaggcga
     1201 aaactttggc gaataccacc gagccgaccg cgtaggtcgc ggtcaccgcg cccatcgcga
     1261 agccccgccg tcgcggcatc acccggccgg ccagctgcag gcagtaaccg taaccgatgc
     1321 cgttactgag cccgaacacc aggctgtaac cgacgcacag cgcccaccac gcgggcacca
     1381 gtgccgccag cgcggtaccg gcggcagcgg ccaggcaggt cagcaacacc aggctggctg
     1441 gcgcgagccg atggtagatt cgatacccga ccaataccga gaccgtcagc gcgaccagcg
     1501 ccagcgagta aatcaggctg atctcggcgc gcggcaggcg cagcaaggct tcgagcggcg
     1561 cgatgaatac cgagaacgcg tgcaccgagc cgagcacgaa ggtgaccagc atcgccgcgg
     1621 caacggccag gtagggacta ttcaatgatc accggctccg ccgcctgccg gcgccgctgg
     1681 cggcgcgcga agatgaagat cccgctaccg gcaatcaccg ccgcaccgat ccaggtcgag
     1741 agcaccggca cctcacccca gaacaggtaa ccgacgatcg cggcgaacac catcgccagg
     1801 tactcgtagg gcgccagcag gctggcctcg gcatagcgaa aggagatcgt cagcagccat
     1861 tgctgcaccc cgcagccgag gccgaagacg acgaacaggc cgaggtccgc gccgcgcggc
     1921 gtcaaccagc cactggcaag cacccagccg aggcacagca acaaaccgag gttgttgtag
     1981 taaatgccga tcgctgccgg gtgttcgcta cggctaagct tgcgcagcca gatcgccacc
     2041 agcgcgccgg tgaacgccga accggcggcc gcggcgacgc cgatgtccca gccgcctgcc
     2101 accggccgcg cgatcagcaa cacgccggcg aaaccggcga tcaccgccag ccagcgtcgc
     2161 aggccgattt tttcgccgag caggaagatc gccagcacgg tgatgaagat cggtgccgcg
     2221 taggccagcg cggtggcatc ggcgagcggg atgcgggtga tcgcgaagta caacagggcg
     2281 aggctggcga taccgctgac actccgaatc gcatggtcca gcggccggcg cgtggccagc
     2341 gcggcgaggc ccgcgccaca gcccaggatg acccagaaca gcaggctggc gaacaggtag
     2401 cgaaacagca gcacctcggc gagcgggtaa tcgcgctgca ccagctcgcg caccgcggcg
     2461 ctgagcagtg ccattaccag caggttgccg gtgaaggtcg cgacaccgag cgccggccgg
     2521 tcgcggtgcg cgggggtctc gaacagtgat ggattggcgc tcggcatcgg cattcgtcag
     2581 ggcagcgggt cgcagtgctc gggcgcgagc gccgggctct tgcgctgcag ggattcgcgg
     2641 tgaatcacgt acaggccgca gccgatgatg atcgcggcac cgagccagac ctcgtacggt
     2701 ggaatgtccc gccacaccag gtagccgatg atcgtcgccc acaccagcgc ggtgtactcg
     2761 aacggcgcca gcaccgatac ctcggcgcgc gcgaaggcgg caacaaacac gtagtgcgcg
     2821 accagtgcga tcaatgccat cagcaacaat tgcacgatca ccagcgcatc gagcggcacc
     2881 cagaaccggg gcagcagcgc gctggcgacg atgcccatgc cgaggtgcag cgaaaatgcc
     2941 agcgagatga ccgaatcacg ccgcgacagc tgcctgccgg taatgaagat cagcgcgtag
     3001 accgtggctg cgcacagcac cagcaggtaa gccggcagcg ggccgccccc gcccgggttg
     3061 acggcgatca ccacgccacc caagccgatc accaccgcgc tccaacggtg tacgccgacg
     3121 cgctcgccga gcagcagtgc cgaggccgca gtcaatatga aggcgccgct gaagaagacc
     3181 acggtcgcat cggccagcgg cagtgattgc agtgcgctga agaaactcag cggtgcgacg
     3241 aagccgacca tgccgcgcag caggtgatgc cagggcttgc gcgtcacccg ttcgccgagt
     3301 tcgccacgca gcagcaggat cgacggtatc atcacggtga tgaaccagct gtataccgcg
     3361 atcacctgga gcgccgacat gtcggcgctg accagcagct tggccaccgc gtccatgacc
     3421 tcgaacaaca gcatcgccag caacatcagg ccgataccga gcagattgtt ggattggtaa
     3481 cgtgacaccc ggcgcccgcg ctgcatagca ggagcccgag gttaccttgc cgccgggatt
     3541 agcgcgagaa gtttatgatt cgtgaccgcc ggcgaagccg aaattattct tgccgcggtg
     3601 cttgacccgg tacatcgcct ggtcggccga gcggatcaat tcctcggcgg tgctgccgtc
     3661 gcccggataa aacgcgatgc cgatgctggc actgactgtg acctcgtccg cctcgatgcg
     3721 cagcggccgc gagatatggc ggatcaggct gctggcgata cgttcggcga tcgagcgatc
     3781 cggcagtccg gaggagataa tgacgaactc gtcaccgccg atacgcgcga ccgtgtcggt
     3841 ttcacggatc tcctcggtga tacgctcgga gatcgttttc agcacccggt cgccgaattc
     3901 gtggccgtgc gcgtcgttga cttccttgaa accgtcgaga tcgagaaaca tgaccgacga
     3961 cagttgccgt ttacggcgcg cctcggcgag cgaatgatcg agccgatcct tgcacagccg
     4021 caagctcggc agcccggtga gtgcatcgtg gtttgccagg aaagcgagtt cggcagagat
     4081 cttttcgcgt tccttgatct cggcctgaag ttgcccgacc gtttcggcca gcttgcgcgt
     4141 gcggttcttg accatttgct cgagcgagtc acgagcttcg cgcaagtgct tttcggtgtt
     4201 cttttgctcg gtgatatcct ggatgacgcc gatcgattcg cggggctcac cgccgggacc
     4261 gggcaccgag gtaacctgct cgcgaatcca gcgaatcgcg ccgtccgaac ggtaaatccg
     4321 gtattcgata ttgaggtcct cgcccgttcg cctgtgctgg tcatacgctt cgagcacgcg
     4381 ctcgtaatct tccgcgtgca gatcgtcgat atcgtcgttg cgggagttca ccgtgctctt
     4441 gtactcctcg acgctgagcc cgtgaatacg ggcaaagccg gggctcaggt aggcataggt
     4501 ttcgctgacc tggtcgaaga tgaaatggcc gatctcggtg atcgcctcgg cctggctggc
     4561 caactcgtcc ttgtacagca gttcctgctc gatgattttt tgcggcgtaa tatcctggat
     4621 cacgccgagc gtctggatga cgcgcccgtc acgctttttc ttggcacagg tcagttcgcg
     4681 caaccagcgg accgagccgt cgtcgcgccg aatgcgaaac tcgagcacgc aatcctcgcc
     4741 gtcatcgata taggcgcgat aggcctccgc cacccgttcg cgatcctcgg gcaggatgtc
     4801 ggcgagatca tcctcggagc tggacacgtc gcgcatgtag gcctccgcac tcgtgccgta
     4861 gatacgcgcc aggccatcgc tgacatagac ataccgctgg ttttcttcgt cgtagatgaa
     4921 gtggccgatg tcggtgagtg cttcggactg gcgtgcgagt tcgtcgcgat actcgagatc
     4981 gcgctcgtac ctgaccgtgt cgctgatgtc ctgcaggata ccgaaagcgc cgatcgcgac
     5041 gccctggtcg tcgatgacct cgaccgcgca ttcgcgaata tgccgaatct cgccggatgc
     5101 gcatcgcagg cggtagacgc ggtcgaccgc atgttcccgc cttagtgtct cactggcctg
     5161 ctcgaagctt tcgacatcgt cgggatgaac ttgccgcata aatcctgcga gagaaccgtg
     5221 ggcgcgcagc acttgcttga cggacatgtc atggatacgc gcatactctt cggagcaggc
     5281 cagcagacat tcgagctggt aactccactc gtaatggccg atatgcgcga taagctcggt
     5341 cctgtcgagc agcgcctgct ggtcgcggaa acgggccagc tccgcttcca gcgcgggttc
     5401 gacatcgaat gtcagcttcg ggttattcac tcgtggcgat ggattccgtt cagcgtcgag
     5461 ccatgccagg tggcatgagg atcaatgaac atcggtgtta gtctaagtat atacgcgatc
     5521 cccggcattt ccatgcggct cctttcacgc cgctggacgg tgataaacgc cgtgcaatgt
     5581 agcataatcg acagatcagc atttcgaccg aggccaaccg ccatgaatcg acccgccacc
     5641 cgggtttcgc agcgtgaaga cgtcatcagg caattgcgcg aactgctggg cgaacgcacc
     5701 agcaccaacg agtcgctgct cgagcagcac ggccgcggcg agtcctggca cccggtgcag
     5761 gcacccgacg tcgtctgctt tccgcaacac aacgacgagg tcgcggcgat cgtcaggcta
     5821 tgcgcggaga gcgcgacgcc ggtgatcgcc tacggcaccg ggacttcgct ggaaggccag
     5881 gtgcaggcgc tgcagggcgg cgtatgcatc gacctgagcc gcatgaaccg cataatcgag
     5941 gtcaataccg aggacatgga ttgccgcgtg caggccgggg tcacgcggcg cgagctgaac
     6001 cactacctgc gcgacagcgg gctgttcttc gccatcgatc ccggtgccga tacctcgatc
     6061 ggcggcatga ccgcgacgcg cgcgtcggga accaacgcgg tgcgctacgg caccatgcgt
     6121 gaaaacgtgc tcgggctgac cgtggtgacc gcgaacggcg aaatcatcca taccggcacc
     6181 cgcgcgcgca agtcggccgc cggctacgac ctgacccgcc tgttggtcgg ttccgagggc
     6241 accctcggcg tgatcaccga ggtctgcctg cggctgcacg gccgcgccga ggcgatctcg
     6301 gcggcggtgg tcaatttccc ggatgtgcgc tcggccgccg aggcaaccat tctgaccatt
     6361 caaagcggca ttccaatggc gcgtatcgag atcctcgaca gtgcctacgt cgcggcgatc
     6421 aatgcctaca gcgataccga ctaccccgag ctcgataccc tgttcctcga atttcacggc
     6481 acccgcgccg gcgtcgccga gcaggtgcga atgttcgagg aaatttcacg cgagttcggc
     6541 gcctcgggct ttcaatgggc cgagcgcgag gaagagcgca accgcctgtg gcgcgcgcgc
     6601 cacgatgcct attacgccgg cctcgccgcg cgccctggtt accgcggcta cgtcaccgac
     6661 gtctgtgtgc cgatctcgcg actcgccgac tgcatcgccg agacgcaggc ggatatcgcc
     6721 gaatccgggt tgttcgcgcc gatcatcggc cacgtcggcg acggcaattt ccactccacc
     6781 atcttcatcg atccgaacga cgaagcgatg ctcgccaaag ccctcgagct ggaccagcgc
     6841 atggtgcggc gcgcgcaggc gatgggcggc acctgcaccg gcgagcacgg catcggtatc
     6901 ggcaagctca agcacatgcg taacgagcac ggcgacggcg cgatcgtggt aatgcaggcg
     6961 atcaaggcgg cgctcgaccc gcaaaacatc atgaaccccg gcaaggtcgt cgactgttag
     7021 cttgcctggg ccgcatggac gcactgttat ctggccctga tccgaaggct attgtgcggt
     7081 cattatcagt ctgactgccg aggccttcca acacgcctcc tgatacgatt ccatttcgag
     7141 tatcggaagc atcactgcaa aaggtgccga gtcgagcccg gcaggacgcg gattgcggcg
     7201 gcacgatgca agtcgactct tcatctcggt cgtcgagccg gggcctattc ccggggcttg
     7261 ttgaaaggcg cacgtagagc ccgtggccat gcccgcgtca tcccgcgacg cggcgataaa
     7321 ttgcggctgg cggcgcagtg acagcaggtt gagctcatgc tggtcggctt agttaccccg
     7381 aagggcgggt tccgcgacag gcgccgcaat accctccata tcgccgcaga gctttaccag
     7441 ctggattgca ggcgtcagtc ggactccagg gccagctgtt cgacgtggcg ctggagagcg
     7501 gggtaaaact cgaagaaacc ctcgcaaatc aggtcgtggt ggcgctgtaa atcctcgatc
     7561 gcgccggcaa agcggttttc gaagcgaatg cgtccggcga cgcgatccag cgattcggcg
     7621 atcgcgtcga gatcgcggta agagccgaac cagtcgtatt cgatcatgcg gcgggttacg
     7681 cggcgcatgt tttcgcccgg catcagcccg cggccttttt gcatgcgccg gtaaaagtcc
     7741 gcgatcaacc ggtcgaggtc gcgctgctcg aattgccgcc agtgattgat caacaggtga
     7801 tcgaagtaga tatcgagcgc gataccggcg aagcgccgcc gcgagctggg aaatgcccgc
     7861 ttcatcgcct gcacccgcgg atgcgcatcg gtaaagcgat cgacggcacg atgattgtgc
     7921 agcccggcgc gtaccgcgtg cggcaggcgg tcgacatcga ccccgcgcgc gaagtcgccg
     7981 agcaggttgc cgaccgtcga ttccagcgtc ggctgcgcca gcaccaggtg ggcgaaatga
     8041 ttcatgggcg atactatagg cgatcgcggc gatcgggaaa aatgcattgc cggtgcgcag
     8101 cgactacggt ttaattcgct ggcggtcgaa agctaaggag gagaacatgc cgatttctgt
     8161 cgtcgatacc gagaaacagt ccagcggcgc cgacccttac cgcgagcagc gcgccgacct
     8221 cgccgccgcg ttccgcatgg cggcacgcct cggcatgcac gagggcgtcg ccaatcattt
     8281 cagcctggcg gtgtccgacg acggcaagcg cttcctgatg aatcctttcg gccgccattt
     8341 cgccagcgtc cgtgccatcg aactgctgct gctcgacgcc gacgacgaca acacgatgga
     8401 gcgcgccgac cgcccggacc cgaccgcctg ggcaatccac ggtgcgatgc accgcaacct
     8461 gccgcaggcg cgctgcgtgc tgcacgtgca ttccaagtat gccaccgcgc tggcgtcggt
     8521 cgaggtcgac gggctgccgc cgatcgacca gaacacgatg cgttttttca accgcgtatc
     8581 gctcgacgat ggtttcagcg gcatggggct cggcgaagag gccgaacggc tgacgacgac
     8641 cctcggcgac aaccgcgtgc tgttgatggg caaccacggc gtcatggtgg tcggcgaaaa
     8701 cgtcgcccgc gcgttcgacg agctgtatta cttcgagcgc gcctgcgaga cctacgtcac
     8761 cgcgctggcc accggcaaac cgctgcgcat cgccagcgac gcggtcgccg agactaccgc
     8821 gcgccagtgg gaggattacg tgcagcgggt cggcatcgcc gacacccacc tgcgcgaact
     8881 gcgcgccatc ctcgaccgcg aggaacccga ctaccggctg tgatgcgatc cgcgcggtcc
     8941 gcggaccacg gcaatactgc tatactgaac gccgtttaaa accggtattc actatgtccg
     9001 ccagcgtctc ggcatggctt caggagctcg gcctggagca gtacgccgaa ctcttcgaag
     9061 ccaacgacat cgactgggaa ctgctcgcgg acatcgacca gcagacgctt cgggaaatcg
     9121 gcgtgcaatc ggtcggacac cgcattcgca tcctcaaggc aatcggcaaa ctgcaggccc
     9181 cggccgccct ggccccagcg gccgcagccg cgcccggcga ggctgccgcg gacccggata
     9241 tcaccgcctg gcagcgccgg cccggcgaac gcaagccggt gacgatgctg ttcgccgacc
     9301 tggtcggttc gaccgcactc accgaggaac tcgatgccga ggaggcgcac gacctggtct
     9361 acctggccac gcagcgcatg tgccgagcgg tggaaagcaa caacggcacc gtgtgccgct
     9421 tcatgggcga cggtatcatg gcgatgttcg gtgcgccgct ggccagcgag cgccacgcgc
     9481 tcgaggcatg ccgcgcggcg ctcgcgatgc aggccggcat cgccgagtat gctgcgcaac
     9541 tcgatggcga tcacgcagac cgcgtaaaga ttcgtgtcgg tctgcactcg ggcgaggttg
     9601 tcgtgctcga ggtcggcgac gatccgcaaa accccgagta cgatgcttcc gggccgaccg
     9661 tgccgctggc ggcgcgcatg gagcagtcgg cgaaccccgg caacatcctg atcagcgccg
     9721 aaacccgtat gctggccggc gccgcgatcg ctacccggga tcatccaccg gtggcggtca
     9781 agggattttc cgaagcggtc gaagtcttcg agctcgagcg aatcctgtcg gccgccgaat
     9841 cggcacgggt ggcgatcgaa aagccgatca tcggcaggcg ctcggagctg gcccagtttc
     9901 gcggcctact ggagagctgc gtcgaggaca acagcggcca gtgcctgcac gtgcgcggcg
     9961 aggccggcat cggcaagacc cgcctggtcg gcgaaatgac ccgcctggca caatcgcggg
    10021 attacctgac ccacaaggcg ctggttctcg atttcggtac cggccgcggg cagggcgcga
    10081 ttccatcgct gctcaagagc ctgctgggca ccgacgaaaa tgccgccggt ccggaatgcg
    10141 ccgcggcgct ggcgcgggcg atcagcgatg gcttgctgga cgagcaaaac cgcgtgttca
    10201 tgaacgacct gctcgacctc gagcagccgc tggaactgaa gacgctgtac gacgcgatgg
    10261 acgtcgaaac ccgcaacgag ggcaagcgcg tggcgctggc ggaattgatc aaccggctcg
    10321 cggcacgctc gccgctggcc atcgtcttcg aggacctgca ctgggcggat gccgctaccc
    10381 tcgattacct ggcgagtatc gccgccgcgg tcgtcgacag cgccgtgctg ctggtattca
    10441 cgtcgcgcgt ggagggcgat ccggtcgacg aaaagtggcg cggcgccgcc ggcggcattc
    10501 cgatcgtgac ctgggacctg cggccgttgc ggcgcgcgga atgcctgcaa atcgtgtcgg
    10561 cgttcaccaa taccgacgtc gcgcatatcg agcgctgtat cgagcgcgcc gacggcaatc
    10621 cactgttcct cgagcaatta ctgctcagca gcccggcagg cggcgggcgc atacccgatt
    10681 cgatcaagag cctggtgctg gcgcgcatcg accagttgcc cgtcgccgac aagcgggcgc
    10741 tgcaggcggc ggcaatcctc ggccagcgct tcgatctcga cagcctgctc ttcctggtcg
    10801 atgatcccgg ctacgactgc ggcagcctga tcgagcgaca gctgatcaag cccgaggggc
    10861 cgtactacct gttcgttcat gcgctgatcc aggaaggggc ctacgcgtcg ctactcaagg
    10921 cgcagcgcgc tgcactgcat gcgaaagccg cggcccggta tcgtgaacgc gacgccgaac
    10981 ttcacgccgt gcacctcgat caggccggcg acagcggcgc ggcagccgcc tacctggccg
    11041 cggggcgcga atacctcgcc gcctaccggc ccgaacgggc gctggaattc gcgcgccgcg
    11101 gcatcgagat cgcaccggca gcaacgcgct tcgcgttgaa atgcctggag ggcgaattgc
    11161 tgcgagccat cggcgccacc ggcgagtcga tcgaggtttt tcgcgccgcg atggaactcg
    11221 ccggcgacga cgctgaacgc tgccgggcgc tggtcgggct cgccgaggga ctggcaatca
    11281 tcgaggcgca cgatgaattg gagcgcgtgc tcgccgccgc cgagaccctc gcccgacacc
    11341 tcgattactg gctggaactg gcggtcatcc accagcttcg cggcggcatg tttttcttcc
    11401 agggcgagca cgagcaatgt ctctcggcct gtaatttttc cctcgagtgc gcacgcaaaa
    11461 ccgatacacc ggaggtcgtt gctcgcgccc tcagcggcct ggccgacgcc acctattgcc
    11521 gtggacgctt tcactccgca aaccgctact acaccgagtg catcgacttc tcccgccagc
    11581 acggtctcgg ccgggtgctg gccgccaacc tgtcgatgcg cggttttacc ttccgctacc
    11641 gcaaccaacc cgaagcggct ttgcgagact accgcgaagc tctcgagctg gcggccagga
    11701 cgcgccacct gcgcgccgaa tcggtggcct gttatgccgg cctgtatctc gccgagatgg
    11761 gcgagcacga ggagggtgag gcactgctgc ggcgcggcct cgcgatcagc acgagtctcg
    11821 gttccagcct gctgcagtcg gcggcgctga tcgcgctgtc gcgcctgatc atgaatatcg
    11881 gcgaacgcgc ggaagcgctg tcactggcgc ggcaggctat cgagatcctg ggcgcctccg
    11941 agtccggcat ggcctttcgc ggccccagtg cgcttggcgc gctggccctg gcgaccgaag
    12001 acgacgacga acgccgcgcc gcgctggcgg aaggcaaggc actgctgagt tcgagctcca
    12061 tggggcataa ccatttcaat ttctgcgagg atggcatcga tacctgcctg caaatcggcg
    12121 actgggacgg ggtggactgg ttcaccagcc atctacggga ttacacgcgc gacgagcccc
    12181 tgccattcag cgagttcatc gtcgcgcggg cgaccgcgct ggccgagttc ggccgcggcg
    12241 aacgcagcga gagcctgctg gcgaggctcg aggaattaag cgagcaggcc ggctcgatca
    12301 agctgttttt ccagcggcag gctgtcgacc aggctctcga acaactgcgc gccgccacgg
    12361 ccgccaaacc atcctagggg cggacgaagc cgacatcgaa cacaccgaag ccccggcttg
    12421 ccttacgcct gacctcgata ccgcgccggc gatcaaaacc atttgcggcg cctgaaataa
    12481 accagcatgc cgaccagcac gccgatcatc agcagccaca ccagcgggta gccaaagtac
    12541 cagcgcagct cgggcatcgc ccacgggctg tcgctgtttt gaaaattcat gccgtagacg
    12601 ccgacgatga aggtcagcgg aatgaaaatc gtcgcgatca cggtcagcac acgcatgatt
    12661 tcattcagcc ggaaactgac gctggacagg tagatatcca gcagcccggt aatcacgtcg
    12721 cggtaggtct cgatcagctc catgatctgg atggtgtgat cgtagcaatc gcgataatag
    12781 acctggctgt cgtcgccgat gattccgctc tcgtcccgca gcagcttgct gagaacctcg
    12841 cgctgcggcc acagcatgcg gcgcatgatc aacagggtgc gcttgagccg atgcagtcga
    12901 ggcagcaggt cgcgggtcgg cttttcgagc agttcatctt ccagctgctc gagctcttcg
    12961 ccgagcgact cgagcaccgg gaacccctcg tcgacgacca ggtcgagcaa cgagtagagc
    13021 aggtagtcga taccacggcc gcggattcgc ccggcgttat tgcgcagacg gcgacggacc
    13081 ggcgcaaacg gatcgctggt gcccggatga aaactgatca aaaaattgcg gccggcaaaa
    13141 acgctcagtt gctcgaggct gacgtcaccg ctctccggat ccctcgcggc atgcgtcagg
    13201 gtgatgaaca tctgctcgtc atagtcatcg atcttggggc gttgaccgga attgatcacg
    13261 tcttcgagtg ccagcgagtg caggccgaag accgcgccca gctggcgcat ggtctccggc
    13321 tcggcgtctc cctgcacgtg aatccaggtc agcgtatcgc gctccaggaa ttccctgcta
    13381 tcctcggcgg tggccagttc gcgctcggta aattcgcttg ccgtgtagtc gacgacgagc
    13441 attcgcagcg aactgcccgg ttgcttttcg acttgctgca aggtgccggg agaagttccg
    13501 ggcgggtgat aacgcttcga gaaataggcc atgtcgcatg ctctcgccaa ccgcggcctg
    13561 cgctcgagat gccgcctagg ccggggtttc atcgatgcct caaggtatca tgaacacccg
    13621 gccgattcca tgccgcagcc gggttcggat gaacccgagc atcacggcga ggtcgatcgc
    13681 cgctagcagc cgcgacaatt ttacctggcg gcggaaagtt cgctcagact atcgacgaag
    13741 ttggcattat cgggctcgat ctcgatcgcg cggcgaatcg cgtcgagcga tgctgtccgc
    13801 cgtccctggg cggcaagcgc gtaggcgaga ttgttccaga tcgcagcctc gccgggcgct
    13861 cgttcgatcg ccgcgcgata ggcggcctcg gccgcggcgt agtcgccgag cgcgtaagcc
    13921 gtgttaccga gcccgctgtg cgcgagcaga ttatcgggcc agcgcgcggc ggcggcggta
    13981 taagccttgc gcgcggtgac agtgcggccg atctgttcca tgtcgatcgc agcatcgaga
    14041 aaaacgtcgg cgctggcggt cacgggaatc cgttccggcg gcaccaggac cagcgcccag
    14101 tggccggccc gctgccaggt cttctcgaag gtcgcgaacg agcgcgtggt gcgccggctg
    14161 gtgcccgagc gcaaaatcat ctcgcggtcg tcgaggtcgt aaccgataac gatttcatag
    14221 tgccagaccg gcagcagatc gatggcgagg ttttgtagca cgaataccgg gttgccggca
    14281 tcgagttcgt gcagcagcga ttccatgcga ccatcgagta ccactggcaa caggtcgtac
    14341 tgacgcgcgg cggcgaccac ctcgacctgc aggctgccct tgagctcggg cacgtaaatt
    14401 tgctcggcga tctggtcggg aatcaccgcg ccaccgtgat aattgatgat cgcggccagc
    14461 gctgccggcc cgcagtggta ttcctgctgg ggaaaaaacg gcacctcggt gagctcgact
    14521 cgtggcggaa tcggcggcgg ctcgtcgatc agcagccgcg tctgtggcga ggttgcgcag
    14581 ccgcccagaa acagtgcaat aaaaagcgtg cacgcaaggc cgcgtgcacg ctcgaagggt
    14641 aatttcaagg cgccaatcaa tccttgatga agtcggtata ccccataact tcaagcacga
    14701 tcagtacgat cagcaccacc agcagggcat cagcgccacc ggccggcgcg gcgtcgatgc
    14761 gctggtaaac ttcctgcacc tgggcgtcgg tcatcgaggc cacgcgcgcg gcagccttgc
    14821 tctcgctgac gccgccgacg accagttgtt cgcgaatcca gtcgcgctgt tcgccgaggg
    14881 cagccagttc gatcgccgca ctgccggcct gcagctgcgc ggtgccgacc atggccgcct
    14941 gcgccggaat actcaccgtg agcgcggcga tgccgatcag caggcagcga aaaatcgatt
    15001 taagcaacat gtttttctcc tcatattcaa tcgcgtaaat tgttatcgcc cgcttattat
    15061 agtcgatatt cgatcaggcg agcctggcgc cgttgggcac cctggagtcg ggagtcgcca
    15121 gcaccaccgc gccgtcgtca cggttaaacc ccgtgaccag gcattcggaa cgaaccgggc
    15181 cgatctgctt gggtgaaaag ttgaccacgc cgacgatttg ccggccaacc aggtcgtcga
    15241 ggctatacag gtcggtgatc tgcgcgctcg acttgcgcac cccgatctcg tcgccaaaat
    15301 cgatcttcag cttgtaggcc ggcttgcggg cctcgggaaa ttcctcggcc tcgatgatcg
    15361 tgccgacacg caattcgacc cgctcgaaat cgctccagtc tatggtttcc atatgctctc
    15421 ctcaggaaaa ctctttgcgt attttgtcgc tatgctcgcc gagccccggc acccgtcgta
    15481 accgctctcg cgcgttcgaa cgcagcgccg catgcgcggc gactcgcgcc tcgccagtcg
    15541 gcgtcgcgat gcgttcgcgg cgcagctgcg gatgccgcgc gagaccggcg atgtcgttca
    15601 gccggccaaa ggcgatgtcg gcatcgcgca ggcgctcgac cgctgccgcc tgatcgagat
    15661 ccgcgaacgc gcggccgagc agttcgtcga caaaggcgcg attatcaacg cgcaccttgt
    15721 tgttatgcaa tcgctcgtcg tcggcgagcg actcgtcacc gagcagcccc gcgcagaagc
    15781 ggcgccattc gcgctcgttc tggatcgcga tgacgatgtc gaggccatcc ttacag
//
LOCUS       DATCZV010000469         7614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_569728, whole genome shotgun sequence.
ACCESSION   DATCZV010000469 DATCZV010000000
VERSION     DATCZV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569728"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..939)
                     /locus_tag="VIW27_10620"
     CDS             complement(16..939)
                     /locus_tag="VIW27_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740170.1"
                     /translation="MATASSNSNNEPANGDRRTVDSSEYVYYDGYWIRYYAPLEDTLA
                     NRKRLIDSLTRRAFHHTEPGINTPGKSLDLAREAYLTETDAQRKRVNAAMLAGALFNR
                     ATDLFTSIVDLAELGVKVSENNELMRECSECFQEALTLGQQVRHHSGCEGIDEVWGEP
                     FKAFTMPVADFYESRFIKIAQGMRDIDHIADCMIEVFCGSTMFAAIRQPIVDYAVAAR
                     QIAETMKKDPAIFRVWPNFVALGEELDAFKPEIPADAGQMTRMRVAYGRDLVQEGRRL
                     IGYIAGARVPMPKSTAAYIDKCQRYGKASLP"
     gene            1133..4291
                     /gene="recC"
                     /locus_tag="VIW27_10625"
     CDS             1133..4291
                     /gene="recC"
                     /locus_tag="VIW27_10625"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEY5740171.1"
                     /translation="MPLSILSSNRVETLQASLAQRLVEEPLADPFAREIVVVPTFAMA
                     RWLNLRLAQSQGIAANLHYPQPAQWIWELATDLLDDLPRRDPFSRDNMAWRLFALLPM
                     LQSRPGFEVLAGYLADDDSGIKRWQLAQRIAESFDHCQSCRPQLVRAWGNGEQRQWQA
                     QLWRALVDANGESHRLAIIARLVEELQREGVHAALPPRISLFALSTLAPLYLEALRAL
                     AAQTELCLYLHSPSDQYWADLESEKRRARRRLSHPGQHDLFAAGNELLAAWGRQGQEF
                     QDLLLERAGDLAFDNESFEPPAQNSLLGQVQRSIFSLEAKPRLQQADDSIGLHVCHSP
                     MRECEVLQDRLLDMLQQDESLEPENILVMIPDIAGYAPYVEAVFRRGRLPFNLADVSL
                     ADEHPLVMTFLQLLNLPASRFTLSEILSLLENPALQLRFELDSASLLEIRRLVEQGHT
                     RWGIDAHHKTDFELPATAGNTWQQLNARFFAGFALAGDELWHGIAPLTAGDDASSAAI
                     GRFWHFLDRLRSWRERLSRARPAAQWQALLMRLLDDFFVEPEARESKLQQIRDAIAEL
                     GSAGDSEISSGLLAYLMQRLLQNSEQSGQLYSGGITFCGMRPMRSIPFRIICLLGMNR
                     NDFPRRDPPNDIELSAEQTRPGDPQRRVEDRYLMLETLLCARQRLYLSYTGRSLKDNS
                     ELQPSVLVQELMDFIDAHFDDAEGDARPSAALTQVHSMQAFSTANFDGAEPSYSDYWC
                     RVAQRLEQDDAAPPRPWPLQSLPAGEQGPLVELEQLRRFAADPVRYFFNRRLGIYIRD
                     DDVPDDDEPFVLAGLEAWRLKQQLATDCIAGREPGIARLRAEGLLPHGQAAEAQVERL
                     RGDLAGLLEAVADYADTEARVLTVEVDIDGGNRLTGAIGNFYPGRGLMHFHAGRFKGS
                     RLLGLWIDQLALCAARLPGCEMPAMLVTQDRNWQIPPFDADAARAQLADYCALYRQGL
                     DRPLPVFPEASYEWAGADDRGTALARVRRLWSNRRGGGDRGNPYLALVMQGGYREPFE
                     DAGFDDCAARLYARLLAVAEKR"
     gene            4288..7584
                     /locus_tag="VIW27_10630"
     CDS             4288..7584
                     /locus_tag="VIW27_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5740172.1"
                     /translation="MRQPVLLDARLSLEIDLSGRRLIEASAGTGKTYTIANLYLRHVL
                     EGRAPGEILVVSFTRASTEELQQRIHARLYQAQQLLDTGGHTEDEFLTLLMQRHRCLD
                     PQQQEQRAAALQRALRSMDETMISTIHGFCHAALQDHALLSKRQFESAVTQDDARYWE
                     RALKDWWRQATYSLDYREWALFNRALPGLAMLLDWQRQLREHPRDRVIPEVETPLAQL
                     LRENRDADADQQDDELLNRFRARALFEAGEYARPRVASAKAANAELAYRDQLDLLLAA
                     LAEAGGERLAQRLRERFPVAMIDEFQDTDGVQFEIFQHLYRGQPQTSLTLIGDPKQAI
                     YRFRGGDIFTYIEARDDPSLEIYALNTNWRSTPALITAINYLFTRRADPFIYDHAITF
                     VPVEAAPRGNASPLSLAGDSQPPLTIWQLPRGENGKNLASGEMQKRLHTAVTAEICRL
                     LDTRHAAVIDKRPLHSGDIAVLVRTNDEGEAIRASLGAAGIGSVSIGREGVFASDEAR
                     GLLELLQAVAQPDDEVVLRRARACNLFALDYRALEAEIHDDARWQAWVDALRGLHQAW
                     TDRGFIAMFQQMLEVLDLGNAVAQGSNPERRLTNLSQLGELLQRQSRISPGIEALLSW
                     MQQRMASDADEDSELRLESDARLVKIVTIHKSKGLEYPVVFLPFLWRCTPFTPARDLL
                     RVHDAANRALFDLGSADFDRHLLIAEKERLAEDLRLLYVALTRARSKTYLAWGDAGDG
                     RSRGRPKQTALGYLLHSRQAAADLDTAKPDAFDDASAMLAELAGIAAGCAQIDLRGLP
                     DSTASLVGAEPRHAPLPKAREFTRQLGTAWRINSFSSLTRDVHQAPLAGESGPHGDPI
                     LDFPAGSHVGLLLHEILEELDFERGIDAQGAALIADRAPAYGLDAEAHRQTLARWLQQ
                     ILATDLGPPDLRLGNLGNRRRLNELKFDFALDRCDIDALNRFLQQRCQTPLQPLETPA
                     FRGLMTGIIDLVFEHRGKFYLADYKSNFLGASLDDYRPQALGRAMLERRYDLQALIYS
                     LALHRYLRQRLPDYDYTRHFGGFFYLFLRAMRPASGAARGVYFDCPPRAQIAALDGLF
                     AFTPIPGVPA"
BASE COUNT         1349 a         2479 c         2381 g         1405 t
ORIGIN      
        1 ggcaaatcgt tgaaatcagg gcagcgacgc tttgccgtag cgctggcact tgtcgatata
       61 ggcagcggtg ctcttcggca tcggcacgcg cgcgccggcg atatagccga tcaggcggcg
      121 gccttcctgc accaggtcgc ggccgtaggc gacccgcatg cgcgtcatct gcccggcgtc
      181 ggcgggtatc tcgggcttga aggcgtcgag ttcctcgccg agagcgacga aattcggcca
      241 cacgcgaaag attgccgggt cttttttcat ggtttcggcg atttgccgcg cggcgacggc
      301 ataatcgacg atcggctgac gaatcgcggc gaacatggtg ctgccgcaaa acacctcgat
      361 catgcaatcg gcgatatggt cgatatcccg catgccctgg gcgatcttga tgaaacgcga
      421 ttcgtagaaa tccgccaccg gcatggtaaa cgccttgaac ggttcgcccc agacttcgtc
      481 gataccctcg cagccgctgt gatgccggac ctgttgaccg agcgtcagcg cttcctggaa
      541 acactcgcta cactcgcgca tcaactcatt gttttcgctg accttgacgc cgagttcggc
      601 gagatcgacg atcgacgtaa acaggtcggt ggcgcggttg aacagcgcgc cggccagcat
      661 cgcagcgttg acgcgcttgc gttgcgcatc ggtttccgtg agatacgcct cgcgcgccag
      721 gtcgaggctc ttgccgggtg tattgatgcc cggttcggta tggtgaaagg cgcgcctcgt
      781 caggctgtcg atcaatcgtt tacggttggc cagcgtgtct tccagcggcg cgtaatagcg
      841 aatccagtag ccgtcgtaat agacgtattc gctggaatcg acggtgcgtc gatcgccgtt
      901 tgccggctcg ttattgctat tgctgctagc ggttgccatc gggtccgcgg gatttgctga
      961 catcgtaaac tgacaatgat attcgaatcc gcctacaatg ctatgaattc tgtcggggta
     1021 atcaattaaa atatttgcat gctaggcgca ccgcccgacc gcgagggctc attcagtgat
     1081 cccgttcgaa ggtacaatca tcacgcttcg acaggccgtg aataacccct gaatgccgct
     1141 atccatcctc agttccaatc gcgtcgagac cctgcaggcc agccttgcac agcggctcgt
     1201 cgaggaacca ctcgcggatc cgttcgcgcg cgaaatcgtc gtcgtgccga ctttcgcgat
     1261 ggcccgctgg ttaaacctgc ggctggccca gtcacagggt atcgccgcca acctgcatta
     1321 tccgcagccg gcgcaatgga tctgggagct ggccaccgat ctgctcgacg acctgccccg
     1381 acgggatcct ttctcccgcg acaacatggc ctggcggctg ttcgcactgc tgccgatgct
     1441 gcagtcgcga cctggcttcg aagtccttgc cggctacctt gccgacgacg acagcggcat
     1501 caagcgctgg caactcgcgc agcgtattgc cgagagtttt gatcactgcc agtcctgccg
     1561 gccgcaactg gttcgcgcct ggggcaacgg tgagcagcgg caatggcagg cacaattatg
     1621 gcgcgcgcta gtcgacgcca atggcgaaag ccaccgtctc gcgatcatcg cgcggctggt
     1681 cgaggagttg caacgcgaag gcgtccacgc ggcgttgccg ccacgcatca gcctgttcgc
     1741 gctatcgacg ctggcgccgc tgtacctcga ggcgctgcgc gcactggcgg cgcaaaccga
     1801 gctgtgccta tatctgcaca gcccttccga ccagtactgg gccgacctgg aaagcgaaaa
     1861 acgccgggca cggcggcgcc tttcccatcc cggccaacac gacctgttcg cggccggcaa
     1921 cgagctgctc gctgcctggg gccggcaggg gcaggagttt caggacctgt tgcttgaacg
     1981 tgccggtgac ctcgccttcg ataacgaatc cttcgagcca cccgcacaga acagcctgct
     2041 cggccaggtg cagcgttcga tattcagcct cgaggcaaaa ccgcggctgc agcaagccga
     2101 cgactcgatc ggcctgcatg tctgtcatag tccgatgcgc gaatgcgaag tgctgcaaga
     2161 ccgcctgctc gacatgctgc agcaagacga aagcctcgag ccggaaaaca tcctggtgat
     2221 gattcccgat atcgccggct acgcacccta cgtcgaggcg gtgtttcgac gcggccgcct
     2281 gccgttcaat cttgccgacg tttcgctggc cgacgagcac ccgctggtaa tgactttcct
     2341 gcagttactg aacctgccgg cgagccgttt cacgctgagt gaaatcctct cgctgctcga
     2401 aaacccggca ctgcagctgc gcttcgaact cgattccgcg agcctgctgg agattcgacg
     2461 actggtcgaa cagggtcata cgcgctgggg gatcgatgcc caccacaaga cggatttcga
     2521 gctgccggcg accgccggca atacctggca gcaattgaac gcgcgtttct tcgccggctt
     2581 cgcgctggcc ggtgacgaac tgtggcacgg catcgcgccg ttaacggccg gcgatgacgc
     2641 cagcagcgcg gcgatcggcc gcttctggca ttttctcgac cgcctgcgca gctggcgcga
     2701 acggctgtca agggcccgcc cggcggccca atggcaggcg ttgctgatgc gcctgctcga
     2761 tgattttttc gtcgagccgg aggcgcggga gtcgaagctg cagcaaatcc gcgatgcaat
     2821 cgccgaactc ggcagcgccg gcgacagcga gatatcgagc gggctgctgg cctacctgat
     2881 gcagcgtctg ctgcagaaca gcgagcaaag cggccagctg tattccggtg gcatcacttt
     2941 ttgcggcatg cggccgatgc gcagcattcc gtttcgcatc atctgcctgc tgggcatgaa
     3001 ccgtaacgat tttccacggc gcgatccgcc gaacgatatc gagttgtcgg ccgaacagac
     3061 gcgccccggc gatccgcaac gccgagtcga ggaccgctac ctgatgctgg agaccctgct
     3121 gtgcgcacgc cagcggcttt atctcagtta taccgggcgc agtctcaagg acaacagcga
     3181 actgcagcca tcggtgctgg tgcaggagtt gatggatttt atcgatgccc acttcgacga
     3241 cgccgaaggc gatgcgagac ccagcgcggc gctcacgcaa gtgcattcga tgcaggcttt
     3301 ttcgacagcc aatttcgatg gcgcggagcc ctcctacagc gattactggt gccgtgtggc
     3361 gcaacggctc gagcaggatg acgcggcgcc accgcgtccc tggccgctgc agtccttgcc
     3421 cgccggcgag cagggcccgc tggtcgagct cgagcagttg cggcgctttg ctgccgatcc
     3481 ggtacgctat tttttcaacc ggcgcctcgg catctatatt cgtgacgacg atgtccccga
     3541 tgacgacgag ccctttgtcc tcgcgggact cgaagcctgg cgactcaaac agcaactggc
     3601 caccgactgc atcgccggtc gcgaacccgg catcgcgcgg ctgcgcgccg agggcctgct
     3661 gccccatggc caggcagccg aagcccaggt cgagcggctg cgcggcgatc tggccggctt
     3721 gctggaggcg gttgccgact atgccgatac cgaggctcga gtgctgacgg tcgaggtcga
     3781 tatcgatggc ggcaatcggc tgacgggtgc gatcggcaat ttttacccgg gcaggggatt
     3841 gatgcatttt cacgccggca ggttcaaggg cagtcggctg ctcggattgt ggatcgatca
     3901 gctggcgttg tgcgccgcac gactgcccgg ctgcgagatg ccggccatgc tggtcacgca
     3961 ggacaggaac tggcaaatcc cgccgttcga cgccgacgcg gcacgggcgc agctggccga
     4021 ttactgtgcg ctttaccgcc agggtctcga ccggccgctg ccggtttttc ccgaggcctc
     4081 ctacgagtgg gctggcgcag acgatcgcgg taccgcgctg gccagggttc ggcggctgtg
     4141 gagtaatcgc cgcggtggcg gcgaccgcgg caatccttac ctggcgctgg tcatgcaggg
     4201 cgggtatcgg gaaccgttcg aggacgccgg gttcgacgat tgtgcagctc ggctgtacgc
     4261 gcgattgctc gccgtggcgg agaaacgatg aggcaaccgg tgctactcga tgcccggctc
     4321 agcctggaga tcgacctgtc cggcaggcgg ttgatcgagg ccagtgcggg taccgggaaa
     4381 acctatacca tcgccaacct ttacctgcgc cacgtgctcg agggccgcgc gccgggcgaa
     4441 atcctggtgg tcagttttac ccgtgcgtcc accgaggagt tgcagcagag aatccatgcc
     4501 cggctctacc aggcgcagca gttactcgac accggtggcc acaccgagga cgagttcctc
     4561 accctgctga tgcagcgtca ccgctgcctc gacccacaac agcaggagca gcgtgccgcc
     4621 gcgctgcaac gcgcactgcg cagcatggat gagacgatga tttcgaccat ccacggcttt
     4681 tgccacgccg cgttgcagga ccacgcgctg ctcagcaagc ggcagttcga gtcggcggtc
     4741 acgcaagacg acgctcgata ttgggagcgc gcgctcaagg actggtggcg gcaggcgacc
     4801 tattcgctgg attatcgcga atgggcgctg ttcaaccgcg cactgccggg actggcgatg
     4861 ctgctggact ggcagcgcca gctacgcgag catccgcgcg atcgcgtgat tcccgaggtc
     4921 gaaactccgc tcgcacaatt gttgcgtgag aatcgtgacg cggacgccga ccagcaggac
     4981 gacgagctgc tgaaccggtt tcgcgcccgc gccctgttcg aggcgggcga gtacgctcgt
     5041 ccgcgggtcg cgtcggcgaa ggccgccaat gccgagctcg cctaccgcga ccagctggat
     5101 ctgctgttag cggcactcgc cgaagccggt ggcgaacggc tcgcgcagcg cctgcgcgag
     5161 cgctttccgg tggcgatgat cgacgaattc caggataccg acggcgtgca gttcgaaatc
     5221 tttcagcacc tgtatcgcgg ccagccacag accagcctga cgctgatcgg cgatcccaag
     5281 caggcgattt accggtttcg cggtggcgat atctttacct acatcgaggc ccgcgacgac
     5341 ccttcgctcg agatctacgc gctgaatacc aactggcgtt cgacgccggc tctgatcacg
     5401 gcgatcaact acctgttcac gcgccgcgcc gatcccttta tctacgacca cgcaatcacc
     5461 ttcgtgccgg tcgaggccgc accacgcggc aacgcatcac cactgtcact ggccggtgat
     5521 tcacaaccac cgctgaccat ttggcaatta ccgcgcggcg aaaacggtaa gaaccttgcc
     5581 agtggcgaaa tgcaaaaacg cctgcatacc gcggtgaccg cggagatttg ccgcctgctc
     5641 gacacgcgcc atgcggcggt gatcgacaag cgcccactgc acagcggcga catcgcggtg
     5701 ctggtgcgca ccaacgacga gggcgaggcg attcgcgcca gcctgggcgc ggcaggtatc
     5761 ggctcggtca gcatcggccg cgagggagtg tttgccagcg acgaggcacg tggactgctc
     5821 gagctgctgc aagccgtcgc acagcccgac gacgaggttg tgttgcgccg cgcgcgcgcc
     5881 tgcaacctgt tcgccctgga ttaccgggca ctggaagccg aaatccatga cgatgcccgg
     5941 tggcaagcct gggtcgacgc gcttcgcggc ctgcatcagg cgtggaccga caggggcttt
     6001 atcgcgatgt ttcagcaaat gctggaagtc ctggatctcg gcaacgccgt cgcgcagggc
     6061 tcgaacccgg agcgccgtct caccaacctg tcgcagctcg gcgagctgtt gcagcggcaa
     6121 tcgcgcatct cgcccggaat cgaggccttg ctgagctgga tgcagcagcg gatggccagc
     6181 gatgccgacg aggattccga actgcggctg gagagcgatg cccggctggt caaaatagtc
     6241 actattcaca agagcaaggg tctcgagtac ccggtagtct tcctgccatt cctgtggcgc
     6301 tgcacaccct tcacgccggc cagggatttg ctgcgcgttc acgacgccgc caatcgcgcg
     6361 ctgttcgacc tcggctcggc ggacttcgac cgtcacctgc tgatcgccga gaaggagcgc
     6421 ctggccgagg atttacggct gctctacgtg gcactgaccc gcgcacgatc gaagacctat
     6481 ctcgcctggg gagatgccgg cgacggtcgc agccgcgggc ggccgaaaca gaccgcgctg
     6541 ggctacctgc tgcactcccg ccaggccgcc gcagatctcg acaccgcaaa gcccgatgcc
     6601 tttgacgatg cctcggcgat gcttgccgag ctcgccggga tcgccgccgg ttgcgcgcaa
     6661 atcgatcttc gcggattacc cgacagcacc gcttcgctgg tcggcgcgga gccccggcat
     6721 gctccgctgc cgaaggcccg cgagttcacc cgccagctgg ggaccgcctg gcggatcaac
     6781 agtttttcga gcctgacgcg cgacgtgcac caggcgccgc tggccggtga gtccggtccg
     6841 cacggcgacc cgattctcga cttcccggcc ggcagccatg tcggcctgtt gctgcacgaa
     6901 atcctcgagg aactcgactt cgaacgcggc atcgacgccc agggcgcggc actgatcgcc
     6961 gaccgggcac cggcctatgg cctcgacgcc gaggctcacc ggcaaacgct cgctcgctgg
     7021 ctgcagcaga ttctggccac cgatctcggt ccgccggacc tgcggctcgg caatctcggc
     7081 aaccgccggc ggctcaacga gctgaaattc gatttcgcac tcgaccgctg cgatatcgac
     7141 gcattgaacc ggttcttgca acagcgttgc caaacgccgc tgcaaccact cgaaacgccc
     7201 gcgtttcgcg gcctgatgac cggtatcatc gacctcgtgt tcgaacaccg cggcaagttt
     7261 taccttgccg attacaagag taattttctc ggcgcgagtc tcgacgatta tcggccgcag
     7321 gcgctcggcc gagccatgct cgagcggcgc tatgacctgc aggctctcat ctattcgctg
     7381 gcgctgcacc gctacctgcg ccagcggctg ccggattacg attacacgcg ccatttcggc
     7441 ggctttttct acctgttcct gcgcgcgatg cgcccggcga gcggtgctgc ccgcggtgtt
     7501 tacttcgatt gtccgccgcg cgcgcagatc gctgcgctgg acggcctgtt cgcgtttacg
     7561 ccgatccccg gggtacctgc gtgaagcccg tgctcgagca actggcacgg cacg
//
LOCUS       DATCZV010000470         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_515139, whole genome shotgun sequence.
ACCESSION   DATCZV010000470 DATCZV010000000
VERSION     DATCZV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515139"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VIW27_10635"
     CDS             complement(<1..437)
                     /locus_tag="VIW27_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016400101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740173.1"
                     /translation="MTTLTLAWQSLCNRRLTALLSVIAIALSVALFLGVERLRSGAKE
                     SFTNTISGTDLIVGARAGNVQLLLYSVFRIGNATSNISMQSVEAIAARKDVAWVVPIS
                     LGDSHRGYRVMGTTNDYFEHYRYRNKQHLQFETGRRFEDIFDVV"
     gene            complement(434..1171)
                     /locus_tag="VIW27_10640"
     CDS             complement(434..1171)
                     /locus_tag="VIW27_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740174.1"
                     /translation="MSAVSSAEERNPAGLLALNDVRFHWPGDDGFSLAIDAFTLDYGE
                     KLFMRGPSGSGKSTLLSLICGIVRPDSGSIVIDGTDIAQLSPGRADRLRADKLGIIFQ
                     QFNLLPYGSMLQNVLLPLQFSSTRRERATTGGTAVSEARRLLERLGLQPALHDRGVTR
                     LSIGQQQRVAVARAMIGNPRLIIADEPTSALDADNRDEFLKLLFEEAERASASLILVS
                     HDPALAASFDRQKDIDEIASTLLPGAT"
     gene            complement(1168..1722)
                     /locus_tag="VIW27_10645"
     CDS             complement(1168..1722)
                     /locus_tag="VIW27_10645"
                     /inference="COORDINATES: protein motif:HMM:NF022439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HEY5740175.1"
                     /translation="MVIATPAATAQSAHVHGRGQVDIAIENDRVYLALASPGADIVGF
                     EHAPRNAKQKAAVAAALGKFGNPAELLRFTPAADCRVIRASAELEIDDEHEKHSADED
                     GHGQHEHEAYRQDDNHGGKENHGALVAEYEFACSQIDALAEIEFTYFARFGNAQALEI
                     VLIDGRGQRREDIGRADPVLRLAQ"
     gene            2048..>2198
                     /locus_tag="VIW27_10650"
     CDS             2048..>2198
                     /locus_tag="VIW27_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY5740176.1"
                     /translation="MSLSVVILAAGQGTRMKSSRPKVLHALAGKPLLQHVVDAGRALQ
                     PAQTLV"
BASE COUNT          378 a          649 c          730 g          441 t
ORIGIN      
        1 acgacgtcga aaatgtcctc gaagcgacgg ccggtttcga attgcaggtg ttgcttgttg
       61 cggtacctgt agtgctcgaa gtagtcgttg gtggtcccca ttacgcgata accgcgatgg
      121 ctgtcgccga gcgaaatcgg caccacccag gccacgtcct tgcgcgcggc gatggcttcc
      181 acgctttgca tcgagatatt gctggtcgcg ttgccgattc gaaacaccga gtagagcagc
      241 aattgcacgt tgccggcgcg cgcgccgacg atcaggtcgg tgccggaaat ggtattggtg
      301 aagctttcct tggcgccgct gcgcagacgt tcgacgccga ggaacagggc aacgctgagg
      361 gcgattgcga tgaccgatag cagcgcggtc aggcgtcggt tgcagagtga ttgccaggcc
      421 agggtcagcg tcgtcatgtc gcccccggca gcagggtgct ggcgatttcg tcgatgtctt
      481 tttgccgatc gaagctggca gccagcgccg ggtcgtggct gaccaggatc aggctggcgc
      541 tggcccgttc ggcctcttcg aacagcagtt tgagaaattc gtcgcggttg tcggcgtcga
      601 gcgcggaagt gggttcgtcg gcgatgatca ggcgcggatt gccgatcatc gcgcgggcga
      661 cggcgacgcg ttgctgctgg ccgatgctga ggcgggtgac tccgcgatcg tgcagcgcgg
      721 gctgcagccc cagccgttcg agcagccgtc ttgcttcgct cacggccgtg cccccggtgg
      781 tcgcccgctc gcgccgcgtg ctcgagaact gcagcggcag caacacgttt tgcagcatgc
      841 tgccgtaggg caacaggttg aactgctgga aaataatgcc cagcttgtcg gcacgcaaac
      901 ggtcggcccg gcccggcgag agctgtgcga tatcggtgcc gtcgatgacg atactgccgc
      961 tatccggccg cacgatgccg cagatcaggc tcagtagcgt ggacttgccg ctgccggatg
     1021 gaccccgcat gaacagtttt tcgccgtagt cgagggtgaa agcgtcgatc gccagcgaaa
     1081 atccgtcgtc cccgggccag tggaaacgaa catcgttcaa cgccagcagg ccggcgggat
     1141 tgcgttcctc ggcggacgac acggcgctca ttgcgcgagg cgtaacacgg ggtcggcgcg
     1201 accgatatcc tcgcggcgct gtccgcggcc gtcgatcagc acgatctcga gcgcctgtgc
     1261 gttgccgaaa cgggcgaaat aggtgaactc gatttcggcc agcgcatcga tttgcgaaca
     1321 ggcgaattca tactcggcga ccaaagcgcc atggttttcc ttgccgccgt gattgtcgtc
     1381 ttgccgatac gcttcatgtt catgctgccc gtgcccgtct tcgtctgcac tatgcttttc
     1441 gtgctcgtcg tcgatctcga gttcggccga tgcgcggatt acccggcagt cggcagcagg
     1501 cgtgaagcgc aacagctcag cggggttgcc gaacttgccg agcgcggcgg cgaccgcggc
     1561 cttttgtttc gcattgcggg gcgcgtgctc gaaaccgacg atgtccgcgc ccggcgatgc
     1621 caatgccagg tagacccgat cgttctcgat ggcgatgtcg acctgtcccc tgccgtgcac
     1681 gtgcgccgat tgcgccgtcg ccgcgggcgt ggcgatcacc attgcgataa cgaggccggg
     1741 gagagataaa taatacacgg tgaatttctc cttgcatgcg gtttcgaggt gcgggggtcc
     1801 tgtgcggggc agaccgcggc gccggtatct cgagcgagca acagaggcga attatagaca
     1861 tcgagacgag gaatgcgaat agccgtgact cgatctgcgc ctggcggccc gcgagggctt
     1921 gatcgtgctg ccgaaggccc ggtttcgtga cgcggatcga attgtctgaa ttctacagcg
     1981 ctgcgcgaat gactcgcttc ccgacctgga ctacaatcgt agccggtgta gatcctgaat
     2041 tcggaatatg tctctatcgg tcgtcattct cgctgccggg cagggaacgc gcatgaaatc
     2101 ttcgcgtccc aaggtgttgc atgcgctggc gggcaaaccg ctgttgcagc atgtcgtcga
     2161 tgctggccgt gcgctgcagc ccgcgcaaac cctggtag
//
LOCUS       DATCZV010000471         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_303054, whole genome shotgun sequence.
ACCESSION   DATCZV010000471 DATCZV010000000
VERSION     DATCZV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303054"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            618..914
                     /locus_tag="VIW27_10655"
     CDS             618..914
                     /locus_tag="VIW27_10655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5740177.1"
                     /translation="MAMHVIERRSSPRIHVDGEISYRTDDSSEFRPGEIENLSIGGAL
                     IWVEQQLPADSRLQIRTVPDHEDETVFQFSAIVLCRLREQKGPLWGYSCRFELD"
     gene            complement(937..>2174)
                     /locus_tag="VIW27_10660"
     CDS             complement(937..>2174)
                     /locus_tag="VIW27_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5740178.1"
                     /translation="IAEGVAVFSHTITRPDELPEVLARAWAVFDSARPGPVHIEIPLD
                     VIVENTRRLDAEVRRTEYPPGPDQNAIARAAELLRGARDPFVVLGGGTQDAAAAARAL
                     VELLGAPTILTINAKGVLPAGHALSLGSRLPHPPVLDALARADVVLAIGTELGETDTL
                     LFDAKLELAGKVVRIDIEQQQLTRNIMPHLAICSDAGSAMRALCQALHGHVPADARAR
                     VQALRQAADAHLDDAYRGHGRLLQLIAETLPGVVIVGDSTQPVYGANFLYETDAPRRY
                     FNSSTGYGTLGYALSAALGAKLARPDSAVVCVIGDGGLQFSIGELATAVELGLPLPIL
                     LWNNRGYGEIRRYMTEREIPAIGVDIYTPDFIAIAGGYGCRALRAENAGALRTALAEA
                     AQAAAPTLIEIVEDSFEIP"
BASE COUNT          427 a          723 c          667 g          357 t
ORIGIN      
        1 atccttcgag tagctgaccg gcttctcgca gccggcggcg agcgccccga acagtaaggc
       61 gagcataacg cgatgaatcg aggtttgcat gacattgacc cccatcgttg cggagcgccg
      121 ccggaataaa aagcgatcga acacgcaacc ctggcagcgc tcgatttcat tgaccccttc
      181 attaaaccgc caatcgcagc gcgcgtgttg acttccatca attcaccttc attcgccgta
      241 cgaaacacaa aacaccggtt cgaccggccg atcgcaacag cccttgcggg ccagtaccgc
      301 cgccagcagg caaaagcgcg gcaaaccggc tgcgaaagcc cgtcgtttac cgccggtcga
      361 gacagcggac catggttgcc ggacgacccg ggaggactgg ttcacatttg aaatattttt
      421 ccctcggcga agcatggaac cgttcacgtt ttcctcgcac tcgcctgatt ttgcctggcc
      481 aagcggctac acagttaggg atacggcaat cgtgacctcc accgaagcga ggagaaaggc
      541 cggaacgcga tcccggtcca tcttccctgc ggatgatcga ttggtctttt aattttgaag
      601 ttgcaggaaa attcaacatg gccatgcacg taatcgagcg acgcagttca ccgcgcatcc
      661 acgtcgatgg agagatcagc taccgcaccg acgactcgag cgaatttcgg cccggggaaa
      721 tcgaaaatct gagcatcggc ggcgcgctca tctgggtcga gcagcagctg cccgccgaca
      781 gccggctgca gatacgcacg gtgccggacc acgaagacga aacggtgttc cagttctcgg
      841 cgatcgtgtt gtgcaggctg cgcgagcaaa aaggcccgct gtggggctac agttgccgct
      901 tcgagctgga ctgacggacc gcgagcgaca gccacatcac ggaatctcga acgaatcctc
      961 gacaatctct atcagggtcg gcgccgcggc ctgggcggct tcggccaacg ccgtgcgcaa
     1021 cgcaccggca ttttccgcgc gcaacgcacg gcaaccgtag ccgccggcaa tggcgatgaa
     1081 atccggcgtg taaatatcga caccgatggc cggtatctcg cgttccgtca tgtaacgcct
     1141 gatttccccg taaccccgat tgttccacag caggatcggc agcggcaggc cgagttcgac
     1201 cgccgtcgcc agttcgccga tcgaaaactg caacccgccg tcgccgatta cacagaccac
     1261 cgctgagtcc gggcgagcga gtttcgcgcc gagcgcggcc gacaaggcat agccgagcgt
     1321 gccgtagccg gtcgaggaat tgaagtaacg gcgcggcgcg tcggtttcgt aaaggaaatt
     1381 ggccccgtaa accggctgcg tcgaatcacc gacgatgacc acccccggca gcgtttcggc
     1441 gatcagttgc aacaggcggc cgtgaccgcg gtaggcgtcg tcgaggtgcg cgtcggccgc
     1501 ctgccgcaac gcctgcaccc gcgcgcgcgc atcggccggc acatgcccgt gcagcgcctg
     1561 gcaaagcgcg cgcatcgccg agccggcatc ggaacagatg gcgaggtgcg gcatgatatt
     1621 gcgcgtcagc tgctgctgct cgatatcgat gcgcacgacc ttgcccgcca gttccagttt
     1681 ggcgtcgaac aacagcgtgt cggtttcgcc gagttcggtg ccgatcgcca gcaccacatc
     1741 agccctggcg agcgcgtcga gcaccggcgg gtgcggcagc cggctgccga gactgagcgc
     1801 atgccccgcc ggcagcacgc ccttggcgtt aatggtcagg atcgtcggtg cgcccagcag
     1861 ctcgaccagc gcgcgcgccg cggcggccgc gtcctgggtg ccgccgccga gcaccacgaa
     1921 cgggtcccgc gcaccgcgca gcaactcggc ggcccgcgca atcgcattct ggtccggccc
     1981 tggcggatac tcggtgcggc gtacctcggc gtcgagccgg cgcgtgttct cgacgatgac
     2041 gtcgagcgga atctcgatat gcaccggccc gggccgcgcg ctgtcgaaca ccgcccaggc
     2101 gcgcgccagc acttccggca actcgtcggg acgggtaatc gtatggctga agaccgccac
     2161 cccctcggca atct
//
LOCUS       DATCZV010000472         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_727525, whole genome shotgun sequence.
ACCESSION   DATCZV010000472 DATCZV010000000
VERSION     DATCZV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727525"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="gph"
                     /locus_tag="VIW27_10665"
     CDS             <1..648
                     /gene="gph"
                     /locus_tag="VIW27_10665"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997930.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY5740179.1"
                     /translation="LFDLDGTLIDTAPDMGGALNNLLIEENLAPIPLQQIRPYVSQGG
                     LVLTRLGFAGKVPDDEIETLRRRFLAHYRAIVADQSRLFDGYEAILDAIEARAMPWGI
                     VTNKPEWLTFPLLEQLGLAQRAGVVIGGDSLAERKPHPLPLQVAAERLGIPCERCLYV
                     GDDERDVVAARAANMKSLVAAYGYIEDAAAIPGWNADGVIDHPAELLSHALMVQR"
     gene            645..1379
                     /locus_tag="VIW27_10670"
     CDS             645..1379
                     /locus_tag="VIW27_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020286851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5740180.1"
                     /translation="MTQPPANFDPAPDLLRGRNILVTGATGGLGRAISLDLARHGATV
                     VLLARNLRKVEDLYDEIEAAGCPVPAIYPLNLEGASEQDYAELAQNIEAQLGGLDGLI
                     HAAAMLGAPTVFAHSDAETWYQVHQVNLHAPYLLTRACLPLLSRSPSASLVFVIDDKP
                     GAYWDAYQVSKRGLAAMAELLANEYAGSRLRVNCVNPGKTRTSLQLRAFPAADDNDNL
                     PAPEQRTRVLLYLMSALSTENGELFA"
     gene            complement(1398..2072)
                     /locus_tag="VIW27_10675"
     CDS             complement(1398..2072)
                     /locus_tag="VIW27_10675"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEY5740181.1"
                     /translation="MLRFLAWRLLFEVCNCRRLLGRGRGQVQYLAFGANLSPEIMRER
                     RIEPLAARYFTLRDHGLRFDHPAAWSGCGYASAEPAAGESVHGILYTLSERDAARMDF
                     YEMVPVVDRYRRSWVEQDGERLYFYQTNRSTPDLRPTAEYLGYIVDGLAAHPQVEAAY
                     RETLSRMPTGTPGGYVPTYLGERQPLEPSWLDRLDCGYRKFTLFLFMKILYRLSPTAL
                     FIRQRS"
     gene            complement(2212..>2695)
                     /locus_tag="VIW27_10680"
     CDS             complement(2212..>2695)
                     /locus_tag="VIW27_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740182.1"
                     /translation="LVVEIRMRNSLRGREAFVTLDDRSGRVDVRVSPELLAEIEPILQ
                     KDLVWVVDGGIAFDDFNNGIKLRAVRVQLLDDYRAAHARALHITLNGHAAQQVDALIA
                     ALDDYAARDSMPVVFHLQREGYRYQLRTNGGWSLKPDERCLLALQRCLDGSCFRFEYQ
                     "
BASE COUNT          514 a          890 c          848 g          443 t
ORIGIN      
        1 ctgttcgacc tcgacggcac cctgatcgat accgcgcccg acatgggcgg tgcattgaac
       61 aacctgctga tcgaggaaaa cctggcaccg attccgctgc agcagatccg cccctacgtg
      121 tcgcagggcg gcctggtgct gacgcggctc ggcttcgccg gcaaggtacc cgacgacgag
      181 atcgagacgc tacggcgtag atttctcgcc cattaccggg ctatcgtggc cgaccagtcg
      241 cgcctgttcg acggctacga agcgattctc gatgcaatcg aggcacgtgc aatgccctgg
      301 ggcatcgtca ccaacaagcc cgagtggttg acctttccac tgctcgaaca gctcggtctg
      361 gcgcagcgcg ccggggtcgt gatcggcggt gattcgctgg ccgagcgcaa accgcacccg
      421 ctgccgctgc aggtcgcggc cgaacggctg ggaatcccgt gcgagcgctg tctctacgtc
      481 ggtgacgacg aacgcgacgt agtcgcggcg cgcgccgcca atatgaagtc gctggtcgcc
      541 gcctacggct atatcgaaga tgccgcggcg atacccggct ggaatgcaga cggtgtcatc
      601 gaccatcccg cggaactgct gtcccatgca ctgatggtcc agagatgacg caaccacccg
      661 cgaatttcga ccccgcaccc gatctgctac ggggcaggaa tatcctggta accggcgcca
      721 ccggcggtct cggccgcgcg atatcgctgg acctggcacg gcacggcgcc accgtggtgc
      781 tgctggcgcg caacctgcgc aaggtcgaag acctgtacga cgaaatcgaa gccgctggct
      841 gcccggtacc ggcgatctat ccgctgaacc tggagggtgc gagcgagcag gactatgccg
      901 agctggcgca aaatatcgag gcacagctcg gtgggctcga cggcctgatc cacgccgccg
      961 cgatgctcgg cgcgccgacg gtgttcgccc acagcgacgc cgaaacctgg taccaggtac
     1021 accaggtgaa cctgcacgcg ccctacctgt tgacgcgcgc ctgcctgccg ttgctgagcc
     1081 gctcgccgtc ggcatcgctg gtcttcgtga tcgacgacaa acctggcgcc tactgggatg
     1141 cctaccaggt tagcaaacgc ggcctggcgg cgatggccga actgctggcc aacgagtacg
     1201 ccggcagtcg cctgcgtgtc aactgcgtca acccgggaaa gactcgcacc tcgctgcaac
     1261 tgcgcgcgtt tccggccgcc gacgataacg acaacctgcc cgcccccgaa cagcgcaccc
     1321 gcgtcttgct ctacctgatg agcgccctgt cgactgaaaa cggcgagcta ttcgcctaat
     1381 cgcaaggcgc tgcgtaatca ggaccgctgg cgaatgaata gtgcggtcgg cgacaggcga
     1441 tagagtattt tcatgaacag gaacagcgtg aatttgcgat agccgcagtc gagtcggtcc
     1501 agccaggacg gctcgagagg ctggcgctcg ccgaggtagg tcgggacata accaccgggc
     1561 gtcccggttg gcattcgcga cagggtttcg cggtaagccg cttcgacctg cggatgcgct
     1621 gccagcccgt cgacgatata accgagatac tcggcggtcg gcctcaggtc gggtgtcgat
     1681 cgattggtct gataaaagta cagccgctcg ccgtcctgct ctacccagct gcgccggtag
     1741 cgatcgacca ccggcaccat ctcgtagaaa tccatgcgcg cagcgtcgcg ttcgctgagc
     1801 gtatacagga tgccgtgtac cgattcgccg gccgccggtt cggccgacgc gtagccgcag
     1861 ccggaccagg ccgccggatg atcgaaacgc aacccgtgat ccctgagcgt aaaatacctc
     1921 gcggccagcg gctcgatgcg ccgttcgcgc atgatctcgg ggctcaggtt ggcgccaaag
     1981 gccaggtatt gcacctgccc acgaccgcgg ccgagcaggc gccggcagtt gcacacctcg
     2041 aacaacagcc gccaggcgag aaaacgcagc atggtggatc gtccaaatct aacccgcgca
     2101 gattagtcag atccgcgagc ccggtcaatc aagcgccagc agctatgccc tgcggcaacc
     2161 aggcgacacc atcccgggcg gcgcgctgcc cgataccgcg acggcggccg attactggta
     2221 ttcgaagcgg aagcaggagc cgtcaaggca gcgctgcaat gccagcaggc agcgctcgtc
     2281 gggtttcagt gaccagccgc cgttggtgcg caactggtag cgatagcctt cgcgctgcag
     2341 gtgaaagacg accggcatcg agtccctggc agcgtaatcg tcaagcgccg cgatcagcgc
     2401 gtcgacctgc tgcgcggcat gtccgttcag cgtgatatgc agtgcacgcg cgtgcgcggc
     2461 acggtaatcg tcgagcaact gcacccgcac ggcacgcagc ttgatgccat tgttgaaatc
     2521 gtcgaaggcg atgccgccgt cgaccaccca gaccaggtct ttttgcagta tcggttcgat
     2581 ctcggcgagc agctcgggcg acacgcgcac gtcgacgcga ccgctgcggt cgtcgagggt
     2641 cacgaaagcc tcgcgcccgc gtaaactgtt gcgcatgcga atttcgacca ccagg
//
LOCUS       DATCZV010000473         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_198503, whole genome shotgun sequence.
ACCESSION   DATCZV010000473 DATCZV010000000
VERSION     DATCZV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198503"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..991)
                     /gene="murB"
                     /locus_tag="VIW27_10685"
     CDS             complement(98..991)
                     /gene="murB"
                     /locus_tag="VIW27_10685"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY5740183.1"
                     /translation="MNAAGNPELRGKLLFDEPMSRHTSWRLGGPADRYYVPADLADLQ
                     AYLAGVDEESEILWVGLGSNLLVRDGGIRGQVIAPLNALRELRLEDDGSIYCECGVSC
                     AKLAKFCQRQQLAKADFFAGIPGTVGGALAMNAGAFGGETWAAVEQVVTIDRRGRLHH
                     RGRQDFVIGYREVQVPAGEWFAAARFRFARRASGEQSNIRQLLQQRNASQPIGQPSCG
                     SVFKNPPGKHAAQLIEAVGLKGYCLGTACVSDKHANFIISNDDTLASDVEALIEHIRQ
                     VVDERFGVRLETEVRIVGEAR"
     gene            complement(988..2415)
                     /gene="murC"
                     /locus_tag="VIW27_10690"
     CDS             complement(988..2415)
                     /gene="murC"
                     /locus_tag="VIW27_10690"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY5740184.1"
                     /translation="MSSSGQHFMRRIRNIHFTGIGGAGMSGIAEVFHNLGYRITGSDI
                     AENAMVRHLRELGIEIAIGHRSENLADAHVVVISSAIEAENPELEAARDQRIPVVRRA
                     EMLAELMRFRRGIAVAGTHGKTTTTSLVASVLAAGDIDPTYVIGGQLNSSGRHARLGD
                     SDYLVAEADESDASFLFLQPMMAIVTNIDNDHLSTYEGDFERLKQTFVEFLHHLPFYG
                     LAVVCLDDPVIREIMPRLARPVLTYGESEDADVRIEGITAQGASTRFQLWLPEQSEAL
                     DIELKLTGRHNVLNATAAVAIGWELGVPLDAIAGALAEFSGIGRRCQITDDLSVAGGR
                     IMHIDDYGHHPREIEATLSGIRAAWPNRRLAVLFQPHRYTRTRDLFEDFTRVLSEVDQ
                     LILLDVYPAGESYIKDADGRALARSIRGRGKVDPVFVEELERLDEVVGNLLQDGDIFV
                     TLGAGSIGAWAAEFLQRHQSKGAGK"
     gene            complement(2412..>2938)
                     /locus_tag="VIW27_10695"
     CDS             complement(2412..>2938)
                     /locus_tag="VIW27_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5740185.1"
                     /translation="LVIGGSLGAAALNRIVPAALACLDREQRPLVRHQCGERHLQDCE
                     QHYRDARVEAEVVRFIDDMREAYSWADLVICRAGALTIAELSAVGAASILVPFPYAVD
                     DHQYHNALYLEQQQAAQIMREAELTAENLALKIQFFQQNRRALMAMAERARACFQADA
                     AERLADDILAGASV"
BASE COUNT          521 a          981 c          923 g          513 t
ORIGIN      
        1 agcgacactt cgcgttcggc ggaatcgccg cccatcaaca ccgccacccg gccgcagctg
       61 gccgcggcat cggctgcagg cttgcgcaag ctgatcgtca tcgcgcttct cccacgatcc
      121 gtacctcggt ttccaggcgc acgccgaaac gctcatcgac gacttgccga atgtgctcga
      181 tcaaggcctc gacgtcgctg gccagcgtgt cgtcattact gatgatgaag ttggcgtgct
      241 tgtccgaaac gcaggcagtg cccagacagt agcccttgag tcccactgcc tcgatcagct
      301 gtgcggcatg cttgcccggc gggttcttga ataccgagcc gcaggacggc tggccgatgg
      361 gctgggaagc attacgctgt tgcaacaatt ggcgaatgtt cgactgctca ccggaggcgc
      421 ggcgcgcgaa acgaaatcgt gcggcggcaa accactcacc ggccggcacc tgcacctcgc
      481 gataaccgat aacgaaatcc tgccgtccgc gatggtgcag gcggccgcgg cgatcgatcg
      541 tgactacctg ctcgaccgcg gcccaggtct cgccgccgaa ggcaccggcg ttcatcgcca
      601 gcgcgccgcc cacggtgccg ggaatgccgg caaagaaatc ggccttcgcc agctgctggc
      661 gctggcagaa cttggccagt ttcgcgcagc tcacgccgca ctcgcagtag atgctgccat
      721 cgtcctcgag gcgcagctcc ctgagcgcgt tcaacggcgc gatcacctgg ccgcgaatgc
      781 cgccgtcgcg caccagcagg ttgctgccga ggccgaccca caggatctcg ctctcctcgt
      841 cgacgccggc gaggtaggct tgcaggtcag cgagatcggc gggcacgtag tagcggtctg
      901 ccgggccgcc gaggcgccag ctggtatgcc tcgacatcgg ctcgtcgaac agcagcttgc
      961 cgcgcaattc ggggttaccg gccgcgttca tttgcctgcc cccttcgact ggtgtcgctg
     1021 taggaattcc gcggcccacg caccgatgct gccggcgccc agggtaacga agatgtcgcc
     1081 gtcctgcagc aggttcccga ccacctcgtc gagccgctcg agttcctcga cgaacaccgg
     1141 gtcgaccttg ccgcgaccgc ggatcgagcg cgccagcgcg cgtccgtcgg cgtccttgat
     1201 gtaactttcg ccagccggat agacatccag cagaatcaac tggtcgacct cggacaggac
     1261 ccgcgtgaaa tcctcgaaca gatcgcgggt gcgggtatag cgatgcggtt gaaacagcac
     1321 cgcgaggcgg cggttcggcc aggcggcgcg aatcccggat agcgtcgcct cgatctcgcg
     1381 tggatgatgc ccgtagtcgt cgatatgcat gatcctgccg ccggccaccg acaggtcatc
     1441 ggtaatctgg caacgacgcc cgataccgga aaattcggcc agtgcgccgg cgatggcgtc
     1501 gagcggtact cccagctccc agccgatggc gaccgccgcg gtggcgttga ggacattatg
     1561 ccgcccggtc agcttcaatt cgatgtccag tgcctcggac tgttccggca gccacagctg
     1621 aaaccgcgta ctggcgccct gcgcggtaat cccttcgatg cgcacgtcgg catcttcgct
     1681 ctcgccgtaa gtcaataccg ggcgcgccag ccgcggcatg atttcgcgaa tcaccggatc
     1741 gtccaggcag accaccgcga gaccgtaaaa aggcaggtga tgcaggaact cgacgaaagt
     1801 ttgcttgagg cgctcgaaat cgccctcgta ggtcgacagg tggtcgttgt cgatgttggt
     1861 cacgatcgcc atcatcggct gcaggaacag gaacgatgca tcgctctcgt cggcctccgc
     1921 gaccaggtag tcgctgtcac cgagacgggc gtggcggccc gagctgttga gctggccgcc
     1981 gatcacgtaa gtcggatcga tatcgcccgc cgccagcacg ctggccacca ggctggtggt
     2041 ggtggtcttg ccgtgggtgc cggcgaccgc gataccgcgt cgaaaacgca tcagctcggc
     2101 cagcatctcc gcgcgccgca ccaccggaat gcgctggtcg cgagcggcct cgagttccgg
     2161 gttctcggct tcgatcgcgc tcgagatcac caccacatgc gcgtcggcga ggttttcgct
     2221 gcgatgcccg atagcgatct cgatgccgag ctcgcgcagg tggcgaacca tcgcgttttc
     2281 ggcgatgtcc gaaccggtaa tgcggtagcc gaggttgtga aacacttcgg cgataccgct
     2341 catcccggcg ccgccgatgc cggtgaaatg aatattgcga atgcgccgca tgaaatgttg
     2401 accgctgcta ctcatacact cgctcccgcc agaatatcgt cggccaggcg ttcggccgca
     2461 tcggcctgga agcaggcccg cgcgcgctcg gccatcgcca tcagggcgcg tcgattctgc
     2521 tggaaaaact gaatcttcag cgccagattc tcggccgtga gttctgcctc acgcatgatt
     2581 tgcgcggcct gctgctgttc caggtaaagc gcgttgtggt actggtgatc gtcgaccgca
     2641 taaggaaacg gcaccaggat cgaggccgca ccgaccgcac tcagttcggc gatggtcagc
     2701 gcgccggcac ggcagatgac caggtccgcc cagctatagg cttcgcgcat atcgtcgatg
     2761 aaacgcacca cctcggcctc gacgcgggca tcgcgataat gctgctcgca atcctgcaga
     2821 tgccgctcgc cgcactggtg gcgcaccagc ggacgctgct cgcggtcgag gcaggcgagc
     2881 gccgccggca cgattcgatt gagagccgcg gcgcccaggc tgccgccgat gaccagca
//
LOCUS       DATCZV010000474         7372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_781501, whole genome shotgun sequence.
ACCESSION   DATCZV010000474 DATCZV010000000
VERSION     DATCZV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781501"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VIW27_10700"
     CDS             complement(<1..579)
                     /locus_tag="VIW27_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740186.1"
                     /translation="MRADPPARIVDLAELRAGDDFPCWAAIHAPGHEKPFVLSPKAMR
                     WSDDVFLSRPAECRAFWLMQKKKLRCTLSELFAPLLRHHYGEGYLAVLGAHPWQCLWY
                     LDYQRRQQASGLYLLQSPLDRNIYRGLDWESWFALQQILADHLEATAARGFRRDQFAS
                     RQAQMRRFIERAEIAAPHAMQTAEARSVERRFG"
     gene            complement(842..1489)
                     /locus_tag="VIW27_10705"
     CDS             complement(842..1489)
                     /locus_tag="VIW27_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740187.1"
                     /translation="MASIPLQFTDTGLQQQLQSARALKLRLENQSQVLHSGIEALDEC
                     FADIGPGDLVEWGIPPGLNGRLIPLQLLKQQVPLCLWIYHHHGLGIFASSWISHGVDL
                     ERLFFICSDNPVRELRPVFLEDTFKRIIIDSPRRFSGGDMAFVSRQAREQGQIVFLIR
                     HYFLNPGNGNPHASLRLNTWQSGKRCFTLQPVKGYAGSKVTVPYNAVAEEHGRYN"
     gene            complement(1505..2110)
                     /gene="lexA"
                     /locus_tag="VIW27_10710"
     CDS             complement(1505..2110)
                     /gene="lexA"
                     /locus_tag="VIW27_10710"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY5740188.1"
                     /translation="MQSLTDIQQAVYDYTRQAIEQGRSFPSLRQIANHFGFQHTTARF
                     HLKALEKKGFIRQRSQRVSDYLLAAEPEAEYNGDSFDLVARIPAGAPLPVFDETRESF
                     SVTRDFFGGGDILAIRVTGDSMAGDAIADGDIAIIRRQREANKGDILALRIEDEITLK
                     RMRLENDRARLLPSNPEHAERVVPANQLEILGKLVGIIRKL"
     gene            2179..3834
                     /locus_tag="VIW27_10715"
     CDS             2179..3834
                     /locus_tag="VIW27_10715"
                     /inference="COORDINATES: protein motif:HMM:NF044765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEY5740189.1"
                     /translation="MLPELPRTYYLDNVQTLFAHVGRVYADILDPDMLAFLDDFARLE
                     ADARKLCIRLLNRRHERFRRGKLDYPEIADLDAALDALAAADFVELDGEIDAAELLSL
                     FTRAELLAAHGDDADLKKLRRPQLEAVLLDAADADYFQRLRTDERLIQLLRGDQYLRC
                     QMLFFGNLNQSMTDFVLRDLGLYQYESYTIDAEHRPYRDAVEIEQHWLTYLAESLFEL
                     IDRQDPAALLELCAALPDDIEARAPAWRRGERLRYEIARQLERLGEFDSALDLYRQCQ
                     LPPSRERIARIFDRRGEHRQAFDICAQIATDPFDEAEMQFAASFASRLARRHGFDTPP
                     LVDAINQQHQPEIIDLELEPRDSVELAVAAHLDARAAGERCFYLENHLFTGVLGLLIW
                     DAVFAPLPGAFFNPFQYRPSDFYAHDFCDNRREQLQATWDSLRDNDDILRLVKSRWQQ
                     KHGLMNPLVNWQYLDLEVIELALERIDHAHWRAIFERILADLRNNRAGFPDLLHFPAD
                     GGYCLVEVKGPGDSLQKNQLRWMQYFARHGIAHRLARVTWRAS"
     gene            3819..6146
                     /locus_tag="VIW27_10720"
     CDS             3819..6146
                     /locus_tag="VIW27_10720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5740190.1"
                     /translation="MARELKLGIRELVEFCCRGGDLGGDGSVGVRALEGVRTHQRIQQ
                     RYRHRASAEVALKYNWRIDDVEVELGGRIDLLFDDETPPRIEEIKTVAAHTDEPRGDA
                     VHWAQLRCYGACYARLHELDEVRLCLNHVTLFERREWRRDEIAPRAELEAFVDQTLRR
                     YLRWHALVESRRQTVREQARALQFPFAGFRPRQREFAAGVYRLAGNGGQLMVEAPTGS
                     GKTVSTLFPALKALGEGRCEQLVYLSAKVSGQRQAVAALDRMPSGAACLVIQARARSC
                     PCVTDPGEIDDDGRCRRCIGFFDRLPAAREALFAAGRLDVGDLQRCADEHALCPFELS
                     LQMLPWAEIVVCDFNYVFDPLVQLGYFRSDTRRKLLLVDELHNLVDRARGMYSARLRR
                     AQIRAARAAANGSRIDAALKAMQQALDRELRAQAETRCVTAAPPAALLRACQRFGERI
                     GDEIFANKRIAGETLDFAREVFRFQLIAQLFGDHHRVLGYSSLPGRELKLLCLNAFEY
                     LRDCYPMFTAICGFSATLSPPAYFRRALGLGEECRSLSLEPPFDANRLGVLVGEHIDT
                     RFGRRESQIGAICESIAAVYRARPGNYLVFFSAYHFMQQVHEHFSREFPQIDTRLQQR
                     EFDDAAQAAFIAGFFDGDKRLGFAIMGGRFAEGIDYRGEALIGAILVGVGLPQASLEQ
                     ELIREDFEALRLDGFDYAFRFPGLIRVQQSAGRVIRDERDRGVVVLLERRFRQAAYAR
                     YLPRHWRLQYCNDAESLEQSLTAFWHEAEASDAAN"
     gene            6133..6648
                     /locus_tag="VIW27_10725"
     CDS             6133..6648
                     /locus_tag="VIW27_10725"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY5740191.1"
                     /translation="MPQIDIRQVDITRLDVDAIVNAANNRLEDGAGVCGAIHSAAGPG
                     LLAECRELGGCETGEAKITGGYELPARHVIHAVGPVWRGGDHGEARLLADCYRNSIRL
                     AERHGLKSIAFPAISCGIFGYPLEQAAGIAIETTLQELHHCEHLDEVIFACFSDEIEA
                     ALKRAAAQHAL"
     gene            complement(6684..6986)
                     /locus_tag="VIW27_10730"
     CDS             complement(6684..6986)
                     /locus_tag="VIW27_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEY5740192.1"
                     /translation="MYVIVDICVVPIGVGVSVSEYVVECQRIFEEANLNHEMHAYGTN
                     VEGEWDKVMAAVKRCHEKMHEMGAPRCSSSMRIGTRNDRDQSMRDKMDSVYQKMSI"
     gene            complement(7140..>7372)
                     /locus_tag="VIW27_10735"
     CDS             complement(7140..>7372)
                     /locus_tag="VIW27_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740193.1"
                     /translation="YDAFVHTGCRPAVRAARAAHVPVVVDDAAGPRCQSEWWRLSAAL
                     SAGTMSLAQVAITDPDGGWQWVSRVAVLLTGG"
BASE COUNT         1339 a         2430 c         2302 g         1301 t
ORIGIN      
        1 gccaaaacgg cgctccaccg aacgtgcctc agcggtttgc atcgcgtgtg gcgcggcgat
       61 ctcggcgcgc tcgatgaaac gccgcatctg cgcctgccgc gatgcgaact ggtcacggcg
      121 aaagccgcgc gctgcggtcg cctcgagatg atcggcgagg atctgctgca gagcgaacca
      181 cgactcccag tcgagcccgc ggtagatatt acggtcgagt gggctttgta gcaggtagag
      241 cccgctggcc tgctggcggc gctggtaatc gagataccac aggcactgcc agggatgcgc
      301 gccgagcacc gcgagatagc cctcgccgta gtgatgccgc aataatggcg cgaacagctc
      361 gctcagcgtg cagcgcagtt tttttttctg catgagccag aacgcccggc actcggcagg
      421 gcgcgacaga aagacatcgt cactccagcg cattgccttc ggcgacagta cgaagggttt
      481 ttcatgcccg ggagcgtgaa tcgcggccca gcacggaaaa tcgtcgccgg cgcgcagctc
      541 ggcaagatcg acgatgcgcg ccggcggatc agcccgcatg ttgtacgagc attgcggatg
      601 cccgcgcagg atagttgtcg cagggcgccg gatctggact gagccggcct ggacaggcgc
      661 catagccaga gctatggccc gaaggaaggc caagccatgc ggcaactgga cccgctggcg
      721 ccggaataga atgagtgccg gttacaactt gcgaatgaag gcctgccatc atttgatacg
      781 taaggttttg tcgattcaaa aacatatcag aggcgctcat tcggctcgtg caacatgcgg
      841 gctagttata tcgtccatgt tcctcggcga cggcgttgta gggtacggtg accttgctgc
      901 cggcataacc cttgaccggt tgcagcgtga aacagcgctt gccgctctgc caggtattga
      961 gccgcaggct ggcatgtgga ttgccgttgc cgggattcag aaaataatgc cgaatcagga
     1021 aaacgatctg gccctgttcg cgcgcctggc ggctgacgaa ggccatgtca ccaccgctga
     1081 agcggcgtgg cgagtcgatg atgatgcgct tgaaggtatc ctcgagaaat accgggcgga
     1141 gttccctgac cgggttatcg gagcagatga agaacagccg ctcgagatcg acgccgtggc
     1201 tgatccagct cgaggcaaat atgccgagcc cgtggtgatg gtaaatccac aggcacagcg
     1261 gcacctgctg cttgagcagt tgcagcggta tcaggcggcc gttgaggccc ggtggaatac
     1321 cccactcgac gaggtcgccc ggaccgatat ccgcgaagca ttcgtcgagc gcctcgatac
     1381 cggaatgcag cacctgtgac tggttttcga gccgcaactt gagcgcgcgc gcggactgta
     1441 attgctgttg caggccggtg tcggtgaatt gcagggggat tgacgccatg gtcgcgctcg
     1501 aactctaaag cttgcgtatg atgccgacca gcttaccgag aatctcgagc tggtttgccg
     1561 gcacgacgcg ctcggcgtgt tcgggattcg acggcagcag ccgcgcgcga tcgttttcga
     1621 gacgcatgcg cttgagcgtg atttcatcct cgatgcgcaa ggccaggata tcgcccttgt
     1681 tggcttcccg ctggcggcga atgatcgcaa tatcgccatc ggcgatcgca tcgccggcca
     1741 tgctgtcgcc ggtgacacga atcgcgagaa tatcgccgcc gccgaaaaaa tcgcgggtga
     1801 ccgaaaagct ttcgcgggtc tcgtcgaaca ccggcagcgg cgcgccggcc ggaatgcgcg
     1861 ccaccagatc gaagctgtcg ccgttgtact ccgcctcggg ttcggcggcc agcaggtaat
     1921 cggaaacgcg ctgcgaacgc tggcgaatga aacctttttt ctccagcgcc ttcaggtgga
     1981 aacgcgccgt ggtatgctgg aagccgaagt gattcgcaat ctgccgcagc gacggaaacg
     2041 accggccctg ctcgatggcc tggcgggtgt aatcgtatac ggcttgctga atatcggtga
     2101 gcgattgcat gaattcgaat ataatatata acagcgttgc atgcaacagt gttatatatt
     2161 cgtgtaaact gacaatcgat gctgcccgaa ctgccccgga cctattacct cgacaacgtg
     2221 caaaccctgt tcgcgcacgt cgggcgggtt tacgccgata tcctcgatcc cgacatgctc
     2281 gcctttctcg acgatttcgc aaggctcgaa gcagacgctc gcaagctgtg cattcgcctg
     2341 ttgaatcgcc gccacgagcg ctttcgccgc ggcaagctcg actaccccga aatcgccgac
     2401 ctcgatgccg cgctcgacgc gctggccgcg gccgacttcg tcgagctcga cggcgaaatc
     2461 gatgccgccg agctgttatc gctgttcacc cgcgccgagc tgctggccgc ccatggcgac
     2521 gatgcggacc tcaaaaaact caggcgcccg caactggaag ccgtgttgct cgacgccgcg
     2581 gacgcggatt actttcagcg cctgcgcacc gacgagcgcc tgattcagct gctgcgcggc
     2641 gaccagtacc tgcgctgcca gatgctgttc ttcggcaacc tcaaccagtc gatgaccgat
     2701 ttcgtgctgc gcgacctcgg cctttaccag tacgaatcct acaccatcga cgccgagcac
     2761 cggccctacc gcgatgctgt cgaaatcgag caacactggc tcacttacct ggccgaatcg
     2821 ctgttcgaac tcatcgatcg ccaggacccg gcggcgctgc tggaactttg cgctgcgttg
     2881 ccggacgata tcgaggcgcg cgcgcccgcc tggcgccgcg gcgagcgcct gcgctacgaa
     2941 atcgcgcgcc agctcgagcg gctcggcgaa ttcgatagcg cgctcgatct ctaccgccag
     3001 tgccagctgc cgccgagccg cgagcgcatc gcgcgtatat tcgaccgccg tggcgagcac
     3061 cgccaggcct tcgatatctg cgcgcagatc gcaacggatc cgttcgacga ggccgagatg
     3121 caattcgccg ccagcttcgc gtcgcgcctg gcgcgccgcc acggcttcga cacgccgccg
     3181 ctggtcgacg ccatcaacca gcaacaccag cccgagatca tcgatctcga actcgagccg
     3241 cgcgattcgg tggaactcgc ggtcgccgct cacctcgacg cgcgcgccgc cggcgaacgc
     3301 tgcttttacc tcgagaacca cctgttcacc ggcgtactcg gattactgat ctgggacgcg
     3361 gtattcgcgc cactaccggg cgcgttcttc aatccgttcc aataccggcc gagcgatttc
     3421 tacgcccacg acttttgcga caatcgccgc gagcaactgc aggcgacctg ggattcgcta
     3481 cgcgacaacg acgatattct gcgcctggta aaatcgcgct ggcagcaaaa gcacggcctg
     3541 atgaatccgc tggtcaactg gcaatacctc gatctcgagg tcatcgaact cgcgctcgaa
     3601 cgcatcgacc acgcgcactg gcgtgcgatc ttcgagcgca tcctcgccga tttgcgcaac
     3661 aatcgcgccg gcttccccga cctgctgcat ttcccggccg acggcggcta ttgcctggtc
     3721 gaggtcaagg gccccggcga cagcctgcaa aaaaaccagc tacgctggat gcagtacttc
     3781 gcccgccacg gcattgcgca tcggctggcg cgagtcacat ggcgcgcgag ttgaagctcg
     3841 gcattcgcga gctggtcgag ttttgctgcc gcggcggcga tctcggcggt gacggcagcg
     3901 tcggtgtcag ggccctcgaa ggcgtgcgca cccaccagcg cattcagcag cgctaccgcc
     3961 accgcgccag cgccgaggtg gcactgaaat acaactggcg aatcgacgac gtcgaggtcg
     4021 agctcggcgg ccgcatcgac ctgctgttcg acgacgagac gccgccgcgc atcgaggaaa
     4081 tcaagaccgt cgccgcgcat accgacgagc cgcgtggcga tgccgtgcac tgggcgcagc
     4141 tgcgctgcta cggcgcctgc tacgcgcgcc tgcacgaact cgacgaggtg cgcctgtgcc
     4201 tcaaccatgt aaccctgttc gagcgccgcg aatggcgccg cgacgagatt gcgccgcgcg
     4261 ccgagctcga ggcattcgtc gaccagaccc tgcgccgtta cctgcgctgg cacgcgctgg
     4321 tcgaatcccg tcggcaaacg gttcgcgagc aggcacgcgc gctgcagttc ccgttcgcgg
     4381 gctttcgacc ccggcaacgc gaattcgccg ccggggtgta ccgccttgcc ggcaacggcg
     4441 gacagctgat ggtcgaggcg ccgaccggtt ccggcaagac cgtcagcacc ctgttcccgg
     4501 cgctcaaggc gctcggcgaa gggcgctgcg agcagctcgt ctacctgtcg gccaaggtct
     4561 ccggtcagcg ccaggcggtg gccgcgctcg accgcatgcc gagcggcgcc gcctgcctgg
     4621 tgatccaggc ccgggcgcgc agttgcccgt gcgtcaccga ccccggagaa atcgacgacg
     4681 acggccgttg ccggcgctgc atcggctttt tcgatcgctt gccggcagcg cgcgaggcgc
     4741 tgttcgcggc cggccggctc gacgtcggcg acctgcagcg ctgcgccgac gaacacgcgc
     4801 tgtgcccgtt cgagctgtcg ctgcagatgc tgccgtgggc ggagatcgtg gtctgcgact
     4861 tcaactacgt cttcgacccg ctggtgcagc tcggttattt tcgcagcgac acgcgccgca
     4921 agctgctgct ggtcgacgaa ttgcacaacc tcgtcgaccg cgcccgcggc atgtactcgg
     4981 cgcggctcag acgagcacag attcgggcgg cgcgcgcagc cgccaacggc agccgcatcg
     5041 acgcggcgct gaaggccatg cagcaggctc tcgaccgcga actgcgtgcg caggccgaaa
     5101 cgcgctgcgt caccgccgcg ccgccggcgg ccctgctgcg cgcctgccag cgcttcggcg
     5161 agcgcatcgg cgacgaaatc ttcgccaaca agcgcatcgc cggcgaaacc ctggacttcg
     5221 cccgcgaagt gtttcgcttt cagctcatcg cgcagctgtt cggcgaccac caccgcgtgc
     5281 tcggttacag ctcgctgccg ggccgcgagc tgaagctgct gtgcctgaat gcattcgaat
     5341 acctgcgcga ctgctacccg atgttcaccg cgatttgcgg cttttccgcg accctgtcgc
     5401 cgcccgccta ctttcgccgc gcgctgggtc tcggcgagga atgccgcagc ctgagcctcg
     5461 agccgccgtt cgatgccaac cgcctcggcg tactggtggg cgagcacatc gatacccggt
     5521 tcggccgacg cgaaagtcag atcggtgcga tttgcgagtc gatcgccgcg gtctaccgcg
     5581 cgcgccccgg caattacctg gtgttctttt ccgcctacca tttcatgcag caggtgcacg
     5641 agcatttcag ccgcgagttt ccgcaaatcg acacccgcct gcagcagcgc gaattcgacg
     5701 acgccgcgca ggccgcattc atcgccggtt ttttcgacgg cgacaagcgg ctcggcttcg
     5761 cgatcatggg cgggcgcttc gccgagggta tcgattaccg cggcgaggca ctgatcggtg
     5821 cgatcctggt cggtgtcggt ctgccgcagg cgagcctcga gcaggaattg attcgcgagg
     5881 atttcgaggc cctgcggctc gacggcttcg actatgcatt tcgttttccg ggactgattc
     5941 gcgtgcagca gagcgccggc cgggtgattc gcgacgaacg ggaccgcggc gtggtggtat
     6001 tgctcgaacg ccgttttcgg caggcggctt acgcgcgcta cctgccgcgg cactggcgat
     6061 tgcaatattg caacgatgcc gaatcgctgg aacaatcgtt gacggctttc tggcacgagg
     6121 cggaggcaag cgatgccgca aattgatatt cgacaggtcg acatcacgcg gctggacgtc
     6181 gacgcgatcg tcaatgccgc caacaaccgg ctcgaggacg gcgccggtgt ctgcggcgcg
     6241 atacacagcg ccgccggccc cgggctgctg gccgagtgcc gcgagctcgg cggctgcgag
     6301 accggcgagg cgaaaatcac cggcggttac gagttgcccg cgcggcacgt gatccacgcg
     6361 gtcggaccgg tgtggcgcgg cggcgaccat ggcgaggcac ggctgctggc cgactgttac
     6421 cgcaacagca ttcgcctcgc cgaacggcac ggcctgaaaa gcatcgcctt tccggcgatc
     6481 tcctgcggca tcttcggcta cccgctggaa caggccgccg ggatcgcgat cgaaaccacg
     6541 ctgcaggaat tacaccactg cgaacacctc gacgaggtga tcttcgcgtg tttctcggac
     6601 gagatcgaag ccgcgttgaa acgcgccgcg gcgcagcacg cgctataagc cggggatagt
     6661 ccccggccga cacagagccg cgactagata ctcatcttct ggtacacgct gtccatcttg
     6721 tcgcgcatgc tctgatcgcg gtcgttgcgg gtaccgatac gcatcgagct gctgcagcgc
     6781 ggcgcgccca tctcgtgcat tttctcgtgg caccgcttga ccgcggccat caccttgtcc
     6841 cactcgccct cgacattggt gccgtaggca tgcatctcgt ggttcaggtt ggcttcctcg
     6901 aaaatgcgct gacactcgac gacgtactcg gagaccgaga ccccgacacc gatcggcacc
     6961 acgcaaatat cgactatgac gtacatgatc attctccggc tcgaaaagct ggcgataata
     7021 caccagatat ccgccggcac cgcaagcggc cgacgggacg aggtcgaaaa catcgaagcg
     7081 cgcgttggtc gcggtgcgcc gggatctgcg ccagccggca cccagcgtgg cagtcgtcac
     7141 tagccgccgg tcagcagcac cgcaacccgc gatacccatt gccagccgcc gtcgggatcg
     7201 gtgatggcaa cctgcgcgag cgacatcgtg cctgccgaca gcgcggcact cagtcgccac
     7261 cattcggact gacaacgcgg acctgcggcg tcatccacga ctaccggcac gtgtgccgcc
     7321 cgtgcggcac gcacagcagg ccggcagcca gtatgaacaa acgcatcata cg
//
LOCUS       DATCZV010000475         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_570500, whole genome shotgun sequence.
ACCESSION   DATCZV010000475 DATCZV010000000
VERSION     DATCZV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570500"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="glmM"
                     /locus_tag="VIW27_10740"
     CDS             <1..906
                     /gene="glmM"
                     /locus_tag="VIW27_10740"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629709.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY5740194.1"
                     /translation="KASRMEDAKGRYIEFCKNSIAFKSTLRGVKLVVDCANGATYHIA
                     PAVFRELGAEVIETGTQPNGMNINKEVGALYPQNMRAQVLENGADLGIAFDGDGDRVM
                     MMDARGELVNGDQLLYIIAMSRLASGQLNGGVVGTQMTNLGFEHALAARKIPFQRAAV
                     GDRYVMAKLVEHGWYLGGESSGHIICLDKSTTGDGIIAALQVLEWLIAQQQTLTEACA
                     DMEIYPQTMINVETPRSLDLDSEPRVQDAVRFAETRLGDSGRILLRPSGTEPLIRVMV
                     EGRDSGEVQIIATELAQCVEEAANG"
     gene            1004..1747
                     /gene="tpiA"
                     /locus_tag="VIW27_10745"
     CDS             1004..1747
                     /gene="tpiA"
                     /locus_tag="VIW27_10745"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5740195.1"
                     /translation="MRTKLIAGNWKMNGSLQSVVDLVDGIKAGDAGQAELAVCPPAVF
                     LMKVGGMLDGSNIALGAQNVCDHESGAFTGEVSAAMLVECGCRYAIVGHSERRSLYGE
                     SDELVAARFAMAHRGGLLPILCVGETLDEREQGITEQVVARQLDAVVDSSGIAAFESA
                     VVAYEPVWAIGTGRVATPEQAQAVHAFIRARLAAVDGAIAERLRILYGGSMKPSNAGE
                     LLSQPDIDGGLIGGASLQAEDFLGIAQSV"
     gene            1756..2151
                     /gene="secG"
                     /locus_tag="VIW27_10750"
     CDS             1756..2151
                     /gene="secG"
                     /locus_tag="VIW27_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY5740196.1"
                     /translation="MEQVNSILLVIQVLLSISLIALILLQHGKGADAGAAFGSGASAT
                     VFGARGSGNFMSRATTVVAVLFFIVCLSLAYISSHRAATADGVGGSVTTQELETAPGL
                     QQGGRTEGDLPPAGDLDGGGSQTDLPPAE"
BASE COUNT          406 a          652 c          740 g          411 t
ORIGIN      
        1 aaggcgagcc gcatggaaga cgccaagggg cgctacatcg agttttgcaa gaattcgatc
       61 gctttcaagt cgaccttgcg cggcgtcaag ctggtcgtcg attgcgctaa cggtgcgact
      121 taccatattg cgccggcggt attccgcgag ctcggtgccg aagtcatcga aaccggtacc
      181 cagcccaacg gcatgaacat caacaaggag gtcggcgcgc tctacccgca aaatatgcgt
      241 gcgcaggtgc tcgaaaacgg cgccgatctc ggcatcgcgt tcgatggtga cggcgaccgt
      301 gtaatgatga tggacgcgcg cggcgaactg gtcaacggcg atcaacttct gtatatcatc
      361 gccatgagca ggctcgccag cgggcagctg aacggcggcg tggtcggcac gcagatgacc
      421 aacctcggct tcgagcacgc gctggcggca cgcaagatac cctttcagcg ggccgcggtt
      481 ggcgatcgct acgtgatggc gaagctggtc gagcacggct ggtacctcgg cggcgaatcg
      541 tccggtcaca tcatctgcct cgacaagtcc accactggcg acggtatcat cgccgcgctg
      601 caggtcctcg aatggctgat cgcccagcag cagacactga ccgaggcttg cgcggacatg
      661 gaaatttatc cgcagaccat gatcaacgtc gaaaccccgc gctccctgga tctcgactcc
      721 gaaccgcgcg tgcaggacgc cgtcaggttc gcggaaaccc ggctcgggga cagtggccgg
      781 atactgctgc ggccttccgg caccgagccg ttgatccggg tcatggtcga aggtcgggac
      841 tccggcgaag tgcaaattat cgctaccgag ctggcgcagt gcgtcgagga agccgccaac
      901 ggctgagaac acggccgaaa tcgcgccaaa gccgcaattc cgcattgatt ttcgccgccg
      961 gtgtgagtaa cctttggcgc ctttttagcg agcggtgtta ttcatgcgga ccaaactcat
     1021 tgccggcaac tggaagatga acggaagcct gcagtcggtg gtcgatctgg tcgatggtat
     1081 caaggccggc gacgctggcc aggccgagct cgcggtttgc ccgccggcgg tgttcctgat
     1141 gaaggtcggc ggcatgctcg acggctcgaa tatcgcgctc ggtgcgcaaa acgtctgcga
     1201 tcacgagagc ggcgcgttca ccggcgaggt atcggccgcg atgctggtgg agtgcggttg
     1261 ccgctatgcg atcgttggtc actcggagcg ccgcagtctg tacggtgaaa gcgacgagct
     1321 ggtcgcggca cgcttcgcga tggctcacag gggcggcctg ctgccgattc tgtgcgtcgg
     1381 cgaaaccctc gacgaacgcg agcagggcat taccgagcag gtcgtcgcgc gccagctgga
     1441 tgcggtcgtc gacagcagcg gtatcgctgc gttcgagtcc gcggtggtcg cctacgagcc
     1501 ggtatgggca atcggtaccg gccgcgtggc gacgcctgaa caggcgcagg cggtgcacgc
     1561 gtttattcgt gcacggctgg cggcagtcga cggcgctatc gccgaacggc tgcgaattct
     1621 ttacggcggc agcatgaagc cgtcgaacgc gggcgaatta ttatcgcagc ccgacatcga
     1681 cggcggtctg atcggcggcg cgtccttgca ggcggaggat ttcctcggca ttgcacaatc
     1741 ggtttaggaa ttaatatgga acaggttaac agtattttat tggtcatcca ggtgttgctg
     1801 tcgatctcgc tgatcgcgct gatcctgctg cagcacggca agggcgccga cgccggcgcc
     1861 gcgttcggca gcggtgcctc ggcgaccgtg ttcggcgcac gcggctcagg caatttcatg
     1921 agtcgcgcga cgactgtcgt cgcggtgctg ttttttattg tatgcttgtc gctggcttac
     1981 atctccagcc atcgcgcggc taccgccgac ggtgtcggcg gcagcgtaac cacgcaggaa
     2041 ctcgagaccg cgccggggct gcagcagggc ggccggaccg agggtgacct gccaccggcc
     2101 ggcgatctgg acggcggtgg ctcgcagacc gatttacccc cggccgaata agccgggacc
     2161 atgccgatgt ggtggaattg gtagacacgc tatcttgagg gggtagtgg
//
LOCUS       DATCZV010000476         6974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_463223, whole genome shotgun sequence.
ACCESSION   DATCZV010000476 DATCZV010000000
VERSION     DATCZV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463223"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(467..3898)
                     /locus_tag="VIW27_10755"
     CDS             complement(467..3898)
                     /locus_tag="VIW27_10755"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5740197.1"
                     /translation="MVPPDDAISNHKPSGVDYDFALDDRYTKSDGIVYLTGMQALVRL
                     LIEQIDLDRRQGLRTRGYVSGYRGSPVGAFDSAIWQAGAAITDRGIHFNPGINEDLAM
                     TAVGGTQHVVVTDKADLDGVFGIWYGKGPGVDRSVDAIKHASLAGASPHGGMLMLVGD
                     DQMGKSSSLCHQSEQSLVSGNVPILAPSSVHEYVPLGLHAIAMSRYSGTPTAMKITSN
                     TADSAATVDLSRLVPEFATLPTPPARGSLHYQPGFVPFLEAERILLEERLPAAVAYAR
                     ENRLNSAVIEPREKRLGVVAVGAAYPEVKAAFELMDASDPASLGIGLYKVNFLWPLEP
                     SGILDFASGYDEVLVVEHKRAFVEAQLGRMLLDLSDGERPRLSGKTEPGSSESLLSEN
                     GELNPTQIAAAISARCGVLQVTGLKPLKAQVTLLGTQPKQRIPWYCAGCPHNTSTRVV
                     DGSISASGIGCHSISALMDAENHTWMCQMGGEGQHWVGRAPFSPIKHTFVNLGDGTYN
                     HSGSLAIRSAVSSGVNITFKLLYNDAVALTGGQSVDSDHRPWDIAQQLLGEGVAKIEI
                     VSDRPEDFKHAPWPRGLKVRHRREFDQVQRELREVAGTSVLIYIQTCAAEKRRRRKKA
                     QFPDPPKRMVINERICEGCGDCGLESNCVAIKPLDTRFGTKRKIDQTSCNKDFSCKNG
                     FCPSFVMLEGIDGQDPLKPQNKLLVSLPEEALPTPPVFAGDPTRILVTGIGGTGVITV
                     GAVLSVAARLEGKHALSLDQTGLSQKNGAVASNLTIGTREIRHHPSKIGQGAADLLIA
                     CDLLGGVSDETLTALRTDKARVVANTRVEPLPHFAKDPQARPQGDLLMSRLTAVVPDD
                     NVTTAATSDLAEALVGDGIGANMMLVGFAAQQGLLPVSVEAIQQAIRLNGVAIEMNLA
                     AFTWGRWSVVNPARVHSAASPRLTLAFEQPSTSELLDMHYRELTDYQNRALADVYRQA
                     VTRIDDEDLRRVAANSLFKALAYKDEYEVARLMLAQPNAGAQVTGRVRRFYYLAPPIF
                     GARDADTGRPRKFKMPGWAIEPVFRLLRSLKWLRGGPLDVFAYSEERRNERTLAADCL
                     AMVRAIGEQGASVDREAATRDLSLVLEVKGFGHVKQRNWRHVEQRWQQARQRWSV"
     gene            complement(4396..4471)
                     /locus_tag="VIW27_10760"
     tRNA            complement(4396..4471)
                     /locus_tag="VIW27_10760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4436..4438),aa:Thr,seq:tgt)
     gene            complement(4558..5313)
                     /locus_tag="VIW27_10765"
     CDS             complement(4558..5313)
                     /locus_tag="VIW27_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740198.1"
                     /translation="MKVFFSLLLILNIGFAVFQWLMPYEQMFGETRKIPVAQELQLLT
                     DANTEIVAEPEVVARVEAETDAIAEAVARAEQELAREQARERAKLVTEEQIDKRVCYT
                     IGPITDKARAIELSGRYSGRQVKTEIKSSLEKQYLGVMVFIGGHDSRQAAVDTANRLI
                     KSGISDHIIVDKPNEKNVLSLGVFGLKRNADRLKARVERMNFDVETEARYRERTIFWL
                     YGEQSSEQEMLRLLGEPETRDGISQIPTQCLPT"
     gene            complement(5310..6044)
                     /locus_tag="VIW27_10770"
     CDS             complement(5310..6044)
                     /locus_tag="VIW27_10770"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY5740199.1"
                     /translation="MILLDCGNSSVKAQYRAHGRLQASFATRYRGDWSARLADWLRDL
                     PASHCWLASVLDAGRGQALDAVLGARFGDAITHCRSAAEACGVVSGYDEPGQLGVDRW
                     LALLGAAALVDGDCVVIDAGSAITIDLLRGDGRHLGGAILPGIHTSPQRFRRIFAHID
                     FDDPRIAANDAPGSSTEAAIQLDYPLDSIRRLRELVTDWLPRLDPGAQILLAGGDAPR
                     VQRELARDARIVPDLVFLGMARLAGA"
     gene            complement(6041..6832)
                     /locus_tag="VIW27_10775"
     CDS             complement(6041..6832)
                     /locus_tag="VIW27_10775"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY5740200.1"
                     /translation="MSSAPIDADALAGFAAARGLDWICQQSTASTNADVLQHFERSRR
                     EVVAVSAAQTAGRGRRGRGWHSPPGRNIYCTLGLLKPVPANRQALVGIAAGIALCRSL
                     RAASGAEIHLKWPNDLLLQGAKLGGILTESRSHREQFFFAIGFGLNLALDDADHGAIG
                     QPAASLDRAARRPLERDPLLIALLDAVLAEVRGLDIADEAGLADAFARYDAFHGREVE
                     IVDGAGHRTRGIDRGIAADGQLRLETGAGLESFAAAEISLRAPAA"
BASE COUNT         1345 a         2224 c         2052 g         1353 t
ORIGIN      
        1 gtgccaagat cggaggctaa ttactttgca caaggtatta ctggccacta ccggatgcta
       61 accggttcct ggaattccga aacatctgta atctgaccaa gcgcactggt caccctaaaa
      121 ccaataaacc agtattccgg ttaacccttg atgttcagat tacatccata tccgatttgc
      181 atcgcgtcat tcgacaacga ggcgtcgatg ccgatcccaa cctcggcaga ttgatccgaa
      241 tcccgattga tttgcacggc acgaaacccg attccgggtc gccatttatt cagcgcggtc
      301 atgctattca aatgatcgta taatcgccat tcagggggta ccgccgcatt agcgtcgaaa
      361 ttgtcatcat cttgttacgc cttatacgac ttcgcaagaa gccatgaata ctatgtcctt
      421 tgcgttatcc ggaggtgcta tccccttcgt tctccaggat acccggtcac acagaccagc
      481 gctgcctggc ttgctgccaa cgctgctcga catgtcgcca gtttctctgc ttgacgtgcc
      541 caaagccctt gacctcgagc accaggctga ggtcccgcgt tgccgcttcc ctgtcaacgc
      601 ttgcaccctg ctcaccaatc gccctgacca tcgccaggca atcggccgcc agcgtgcgtt
      661 cattcctccg ctcttcacta taagcaaaga catccagtgg cccgccccgt agccacttca
      721 gggatcgcag caagcgaaac accggttcga tggcccaacc cggcattttg aatttccggg
      781 gcctaccggt atcagcatca cgggcaccga aaatgggtgg cgccaggtaa tagaaccgcc
      841 tgacccgacc agtgacctgc gcacccgcat taggttgcgc caacatcaaa cgcgccactt
      901 cgtattcgtc cttgtacgcc agcgccttga acagcgaatt ggcggctact cgccgcaaat
      961 cttcgtcgtc gatacgagtc accgcctgac gataaacatc ggcaagcgcc cggttttgat
     1021 agtcggtcaa ttcgcggtaa tgcatatcga gcaactcact ggtcgagggt tgctcgaagg
     1081 ccagcgtcaa acgcggtgac gccgcggagt gcaccctggc cggattcacc accgaccagc
     1141 gaccccaggt aaaggccgcc aggttcattt cgatagccac cccgttgagt cgaatggctt
     1201 gttgtattgc ctcgacagag accggcagca aaccctgctg cgccgcgaaa ccaaccaaca
     1261 tcatgttggc accgataccg tcaccgacca gggcttcagc cagatcactg gtggccgcgg
     1321 tggtcacatt atcatcgggc acgaccgctg ttaaacgcga catcagcagg tcaccctgag
     1381 gacgcgcctg cggatcttta gcaaagtgcg gcaagggttc gacccgagta ttggcgacaa
     1441 cacgggcttt gtctgtgcgc aaggcggtca gggtttcatc gctgacgcca cccagcaggt
     1501 cacaggcaat cagcagatcg gctgccccct gcccgatctt gctcggatgg tgacggattt
     1561 cccgagtgcc aatcgtcagg ttgctggcaa cggcgccatt cttttgactc agcccggttt
     1621 gatcgaggct gagagcatgt ttgccttcca gccgggcggc aacagacagc accgcaccca
     1681 ccgtaattac ccctgtgccg ccaatgccgg taaccagaat acgcgtggga tcgccagcaa
     1741 acaccggggg tgttggcaat gcctcttcgg ggagcgagac caataacttg ttctgcggtt
     1801 ttagcgggtc ctggccatcg ataccctcga gcatgacaaa actgggacag aacccgttct
     1861 tgcaggaaaa gtccttgtta cagctggttt gatcgatttt acgcttggta ccgaaacgcg
     1921 tatccagcgg cttgatcgcg acacagttac tctccagacc gcaatcacca cagccctcgc
     1981 atatccgttc gttgataacc atacgcttgg gtggatccgg gaactgcgcc ttcttgcgcc
     2041 gccgccgctt ttccgcggca caggtctgta tgtaaataag caccgaggta ccggcaacct
     2101 cgcgcagctc tcgctgcacc tggtcgaact cgcgccggtg ccgaaccttt agtccccggg
     2161 gccagggcgc gtgtttaaag tcctccggtc ggtcgctgac aatctctatc ttcgcaacac
     2221 cctcccccag caactgttgc gcgatatccc aggggcgatg atcgctatcg acactctgac
     2281 cgccggtgag cgcgaccgca tcgttgtaca gcagtttgaa ggtgatgttg accccgctgc
     2341 tgaccgcact gcggatggcg agactaccgg aatggttata ggtaccgtca cccaggttga
     2401 cgaaagtgtg cttgatcgga ctgaacggcg ctcgcccgac ccaatgctgc ccttcaccac
     2461 ccatttggca catccaggtg tggttttcgg catccatcaa cgcgctgatg gaatggcaac
     2521 cgatgccgct ggccgatatc gaaccgtcga ccacccgggt cgaggtattg tgcgggcagc
     2581 ccgcgcaata ccacggaatg cgctgttttg gctgcgttcc gagcagcgtt acctgggctt
     2641 tgagaggttt gaggcccgta acctgcaata ccccgcaacg cgcgctaatc gccgcggcga
     2701 tttgcgtcgg gttcagctcg ccgttttcag acagcagtga ctcactcgaa cccggctctg
     2761 tttttccgct caatcgaggg cgctcgccgt cactcaagtc aagcagcatg cggcccagct
     2821 gcgcctcgac aaacgcccgc ttgtgctcga ccacgagcac ctcgtcgtaa ccgctggcaa
     2881 aatcgagaat accgctgggc tccagtggcc acagaaagtt gaccttgtac aggcctatac
     2941 ccagtgatgc cgggtcgctc gcatccatca gttcaaacgc ggccttcact tccggatagg
     3001 ctgcgccaac agcgacaact cccagccttt tttccctggg ctcgatgact gcggaattca
     3061 ggcgattttc ccgggcatag gcaaccgcgg caggcaggcg ttcttccaac aggatacgtt
     3121 cggcttccag aaacggcaca aaacccggtt ggtagtgcag gctgccacgc gccggcgggg
     3181 tgggcagtgt ggcaaattcg ggaaccagtc gtgaaaggtc aacggtggcc gcgctgtccg
     3241 ccgtgttgga ggttattttc atggcggtcg gcgtaccgct gtagcgggac atggcgatcg
     3301 catgcaggcc cagcggaaca tattcgtgaa cactcgaagg cgcgaggatg ggtacattgc
     3361 cactgaccag cgactgctcg ctctggtggc acaggctgct gcttttgccc atttgatcgt
     3421 cgccgaccag catcaacatg ccgccatgcg gcgatgcacc ggccaggctc gcatgcttga
     3481 tggcgtcgac actgcggtcg acaccgggtc ccttgccata ccagataccg aaaacaccat
     3541 ccagatcagc cttgtcggta accacgacat gctgggtacc gccgacggcg gtcatcgcga
     3601 gatcttcatt gatgccggga ttgaaatgaa taccgcgatc ggtaatcgct gcacccgcct
     3661 gccagatagc gctatcgaag gcgccaacgg gactgccccg atagccgctg acataccctc
     3721 gggtgcgcaa tccctggcgc cgatcgaggt cgatttgctc gatcaacaac cgcaccaggg
     3781 cctgcatccc cgtcagataa acaataccgt cggatttcgt gtagcgatca tccagcgcaa
     3841 aatcgtaatc gacgcctgaa ggtttgtggt tcgaaatagc gtcatccggc ggcaccatcg
     3901 ccatcctccc cgtctgagat acaagcgccg aatattatca aaactgcgag tgtgtttagc
     3961 tttttattgt tcccaggcgt cgcaggccag gccgcgcttc acggcgcgcc cggcatgccc
     4021 ggagcgccgg ccggcacatc cctgtgcgca agcgcgccga gtgcaaggcg gaattttttg
     4081 cgataccacg agcaggaaaa gatccccgct tacgcgggga tgactcaaaa attgcgatcg
     4141 cggcgcgcag ccgccgaaca gttaaattga atcggaaaat tttgggcgtc gcgagcggcg
     4201 cgccgcaaat tccaacgcgg ctatcggctt gcgcagcagc cgagcggttt caatcgtact
     4261 gaaagttttt tggcgtcgcg agcgccgtga gagcaaggcg gatttttgcg cgataccgcg
     4321 aacagtacaa accagtacgg gagcggttcg cgcaaaaatc caacgccgct atcgcggcgc
     4381 gcagcagcca aaaaatggtg ccggcacgag gaatcgaacc ccggacctac tgattacaag
     4441 tcagttgctc taccaactga gctacgccgg caatttcatt tattcttttt tccgcaaggc
     4501 ttttttattc tagaccatca gccttcaggc ggggccgcaa atattattgt cttcggccta
     4561 ggttggcaag cactgggtcg ggatctggct gatgccgtcg cgggtctcgg gctcgccgag
     4621 caggcgcagc atttcctgtt cgctggactg ctcgccgtaa agccagaaga tcgtgcgctc
     4681 gcggtaacgc gcctcggttt cgacgtcgaa attcatccgt tcgacgcgcg ccttgagccg
     4741 gtcggcgttg cgcttgagcc cgaatacccc gagcgacagc acgtttttct cgttcggctt
     4801 gtcgacgatg atatggtcgc tgataccgct cttgatcagg cggttggcgg tatcgacggc
     4861 cgcctgtcgg ctgtcatggc cgccgatgaa caccatcacc ccgaggtatt gcttctcgag
     4921 actggacttg atttcggtct tcacctgcct gccgctgtag cgcccgctga gctcgatcgc
     4981 gcgcgccttg tcggtgatcg ggccgatggt gtaacagacg cgcttgtcga tctgctcctc
     5041 ggtgaccagt ttcgcgcgct cccgggcctg ttcgcgggcg agttcctgct cggcccgcgc
     5101 caccgcctcg gcgatcgcgt cggtctcggc ttcgaccctg gcgacgactt ccggttcagc
     5161 gacgatctcg gtattggcat cggtcagcaa ttgcagctcc tgggcgaccg gaatcttgcg
     5221 cgtttcgccg aacatctgtt cgtaaggcat cagccactgg aacacggcaa agccgatgtt
     5281 gagaatcagc aatagtgaaa aaaatacttt catgcgccgg ccagccgggc catgccgagg
     5341 aataccaggt cgggaacgat gcgcgcgtcg cgcgcgagtt cgcgctgcac gcggggcgcg
     5401 tcgccgccgg cgagcaggat ttgagccccg gggtcgaggc gcggcagcca gtcggtcacc
     5461 agttctcgca gtcgtcgaat cgaatccagc gggtagtcta gctgaatcgc ggcctcggtt
     5521 gaactgcccg gcgcgtcgtt agctgcgatt cgcggatcgt cgaaatcgat gtgcgcgaag
     5581 attcgccgaa agcgctgcgg cgaggtgtgg atgccgggca gtatcgcgcc gccgaggtga
     5641 cggccgtcgc cgcgcagcag gtcgatcgtg atcgcgctgc cggcgtcgat gaccacgcaa
     5701 tcgccgtcga ccagcgccgc ggcgccgagc agcgccagcc agcgatcgac gccgagttgg
     5761 ccgggctcgt cgtagccgct aaccaccccg caggcctcgg ccgcggaacg gcaatgcgtg
     5821 atcgcgtcgc cgaagcgcgc gccgagcacc gcgtcgagcg cctgcccacg gccggcatcg
     5881 agcaccgagg cgagccagca gtggctcgcc ggcaggtctc gcagccagtc ggcgagacgc
     5941 gcactccagt cgccgcggta gcgggtcgcg aaactggcct gcagccggcc gtgggctcga
     6001 tattgcgcct tgaccgagct gttgccgcaa tcgagcagga tcatgccgcc ggcgcccgca
     6061 gcgagatttc cgccgccgca aacgactcca gcccggcgcc ggtttcgaga cgcagctggc
     6121 catcagcggc gatgccgcgg tcgatgccgc gagtcctgtg tcccgcgcca tcgacgattt
     6181 cgacctcgcg gccgtgaaac gcatcgtagc gcgcgaacgc gtctgccagc ccggcctcgt
     6241 cggcaatatc caggccgcgc acttccgcga gcaccgcgtc gagcagcgcg atcaacagcg
     6301 gatctcgctc gagcgggcgc ctggcggcac gatcgaggct cgccgccggc tggccgatgg
     6361 cgccgtgatc ggcgtcgtcg agcgccaggt tgaggccgaa gccgatggcg aaaaagaatt
     6421 gttcgcggtg cgaccgtgac tcggtcagga tgccgccgag cttggcgccc tgcaggagca
     6481 gatcgttggg ccacttgaga tgtatctcgg caccgctggc cgcgcgcagc gagcggcaca
     6541 gcgcgatccc ggcggcgatg cccaccagcg cctggcggtt ggcgggtacc ggcttgagca
     6601 gccccagcgt gcagtagatg ttgcgccccg gcggcgaatg ccagccgcgg ccgcggcggc
     6661 cgcgccccgc ggtttgcgcg gcactgaccg cgaccacctc gcgccgtgag cgctcgaaat
     6721 gctgcagcac gtcggcgttg gtcgacgccg tcgattgctg gcaaatccag tcgaggccgc
     6781 gggctgcggc gaagccggcc agcgcgtcgg catcgatcgg cgccgatgac atcaaagccc
     6841 cagccacgcc cggcaggcgc cgccgggcgc atcgccgagc gcgagttcga aggtgttttc
     6901 ctcggccgcc tgcagctcta cctcgaggca atgcagttcg tcgcggcgaa acacgtgcac
     6961 tcgccagcgg ccgc
//
LOCUS       DATCZV010000477         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_40477, whole genome shotgun sequence.
ACCESSION   DATCZV010000477 DATCZV010000000
VERSION     DATCZV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40477"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VIW27_10780"
     CDS             <1..264
                     /locus_tag="VIW27_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEY5740201.1"
                     /translation="EAGRLIRRAAEMEHLLAMRVYAYALEEGSLGFASDHAAAVEWFR
                     RAAQAGDTVAMRRLRDAYAEGLLGLPVDPEQAAQWDEKIMQDK"
     gene            277..585
                     /locus_tag="VIW27_10785"
     CDS             277..585
                     /locus_tag="VIW27_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5740202.1"
                     /translation="MRKILLTPGLVLALLVSSQVAASDLANGGKIYRTHCAGCHGPTG
                     KSVMAGVPDFDRPGAVLQSDSSLLTRIQAGKRACPAYRGILREQEIFDVIAYIRSLGL
                     "
     gene            596..1780
                     /locus_tag="VIW27_10790"
     CDS             596..1780
                     /locus_tag="VIW27_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY5740203.1"
                     /translation="MKNLRYLRLLLMSLVLSACSQEEASDAQAPASPATTVEVNVPET
                     STAEPITPPTTLPKIADVFNVGDTVYVRALAIEPATNRLWVGTSLGVNEIDLADFSLV
                     NTFARDHGLANEYVFGIGIDADGYKWFGTNAGGVSRYKDGDWKVFFPMHGLADYWIYS
                     FANAADGGLWIGTWAGANYLDREQEKFDTYVEELINEWVYGIAVDSKGVAWFGTEGGV
                     SRFDGSDWTSWSHDDGIGGENPEALPYSRNTGLGTRSRHDLGVLSGGQPTYNPNYIFS
                     MLADRDDTIWAGTWGGGVARYENQRWVNLMEADGLAGNIVYSMAQDADGVYWFGTNKG
                     LSRYDGEQFTNWNVHHGLPANDVYAIAIAPSGDVWAGTRGGVTRLAMQPSQSLSSSDN
                     AN"
     gene            1770..>2028
                     /locus_tag="VIW27_10795"
     CDS             1770..>2028
                     /locus_tag="VIW27_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740204.1"
                     /translation="MQTKSLLGLIVVIIALVGGAFWLGINQGIDLSDTELRPQPPAPG
                     STANQAPATTAAAATDQTAESAAAAAAPKPGFTHFRVGNRNV"
BASE COUNT          395 a          666 c          636 g          331 t
ORIGIN      
        1 gaggcgggac gactgattcg gcgcgccgcg gaaatggagc atctgctggc gatgcgcgtc
       61 tatgcctatg cgctggaaga gggtagcctc ggcttcgcca gcgatcacgc cgcggccgtg
      121 gaatggtttc gacgggcggc gcaggccggc gacacggttg cgatgcgccg cctcagggac
      181 gcctacgccg agggcctgct cggcctgccg gtggatcccg agcaggccgc gcaatgggac
      241 gagaaaatca tgcaagataa ataagacgga aataacatgc gtaaaatact attaacgccg
      301 gggcttgtcc tggcactgct ggtttcgtcg caggtagcgg cttccgacct cgccaacggc
      361 ggcaagattt accgcactca ctgcgccggg tgtcacgggc ccaccggcaa gagcgtgatg
      421 gccggggttc ccgatttcga ccggccgggc gccgtgttgc agtcggatag cagcctgcta
      481 acccgcattc aggccggcaa gcgagcttgc ccggcttatc gcggcattct ccgcgaacaa
      541 gagatcttcg acgtcatcgc ctatatcagg tcactgggcc tgtaagcagg aaaatatgaa
      601 aaatcttcgc tacctgcggc tgttgctgat gtcgctcgtg ctgtcggcct gttcgcagga
      661 agaagcgagc gacgcgcaag cgcccgcctc acccgcgacc accgtcgagg tgaacgtccc
      721 cgagacgagc accgccgagc caatcacccc gccgacgacg ctgccgaaga tcgccgatgt
      781 tttcaacgtc ggcgacacgg tttacgtgcg cgcgctggcg atcgagcccg cgaccaaccg
      841 gctgtgggtc ggcacctcgc tgggcgtcaa cgagatcgac ctcgccgact ttagcctggt
      901 caataccttc gcgcgcgacc acgggctggc caacgaatac gtattcggca tcggcatcga
      961 tgccgacggc tacaagtggt tcggcaccaa cgccggcggc gtctcgcgct acaaggacgg
     1021 cgactggaaa gtcttctttc cgatgcacgg gctcgccgac tactggattt actcgttcgc
     1081 caacgcggcc gacggcggac tgtggatagg cacctgggcc ggcgccaact acctggatcg
     1141 cgagcaggag aaattcgata cctacgtcga ggaactgatc aacgaatggg tttacggcat
     1201 cgcggtcgac agcaagggcg tggcctggtt cggcaccgag ggcggcgtca gccgcttcga
     1261 cggcagcgac tggacctcgt ggagccacga cgacggcatt ggcggcgaaa atcccgaagc
     1321 gctgccctac agccgcaaca ccgggctcgg cacgcgctcg cggcacgatc ttggcgtgct
     1381 cagcggcggc cagccgacct acaatcctaa ttacatcttc agcatgctgg ccgaccgcga
     1441 cgacaccatc tgggccggca cctggggcgg cggcgtggcg cgttacgaaa accagcgctg
     1501 ggtcaacctg atggaagcgg acggcctcgc cggcaatatc gtctactcga tggcgcagga
     1561 tgccgacggc gtctactggt tcggcaccaa caagggcctg tctcgttacg acggcgagca
     1621 atttaccaac tggaacgtgc accacggcct gcccgcgaac gacgtctacg cgatcgcgat
     1681 cgcgccttcc ggggacgtct gggccggtac ccgcggcggc gtgacccggc tggccatgca
     1741 accctcgcaa tcactctcga gctccgataa tgcaaactaa atcactgcta ggcttaatcg
     1801 tcgtcatcat cgccctggtc ggcggcgcct tctggctggg tatcaaccag ggcatcgatc
     1861 tgagcgacac ggagctccgc ccacagcccc ccgcacccgg ttcgacagcg aaccaggcgc
     1921 cggcaacgac cgccgccgcg gcaaccgacc agacagcgga atcggcggct gccgctgcgg
     1981 caccgaaacc cggctttacc cactttcgag tcggcaaccg caacgtca
//
LOCUS       DATCZV010000478         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_728152, whole genome shotgun sequence.
ACCESSION   DATCZV010000478 DATCZV010000000
VERSION     DATCZV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728152"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIW27_10800"
     CDS             <1..273
                     /locus_tag="VIW27_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740205.1"
                     /translation="ANKQSYLRSWPSGFRVSALSSTRGVKVLLEQRVVSTERLALVPN
                     AAATLHIGGSPVVVHQARAAAVGLFRGMAAPNTSVTPMVAGLAQLL"
     gene            305..2977
                     /locus_tag="VIW27_10805"
     CDS             305..2977
                     /locus_tag="VIW27_10805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEY5740206.1"
                     /translation="MQEFVIGQRWISAAELHLGLGMVIEIEHRTVSIVFPATGETRIY
                     ARADAPLTRVRFREGDWVERQDGEALRVVRVDENAGLIVYRCEDEHGGAADLPEGRLS
                     NFLQFNQPAERLLNAQVDRNKWFNLRCQTREIANRLQQSAVYGLAGSRTSLIAHQLYI
                     AHEVSRRYAPRVLLADEVGLGKTIEAGLILHQQLLLERARRVLIVVPESLVHQWLVEM
                     LRRFNLLFSIFDAQRFAPDDGDQDFVDVAENPFHAEQLVLCTLEFLLAHPQHAAEARA
                     GDWDLLVVDEAHHLEWSPDAASDGYRLVEGLAENTAGLLLLTATPEQLGKESHFARLR
                     LLDPDRFDSLGRFLQQERGYEELARQAEALLEDPERLRELDDLLDRHGTGRVLFRNTR
                     HAVRGFPEREVRPVELEADADEAAYATWLGGFLREYRDRKVLVIAAGADTAISVTERL
                     KQEFGLRATTFHEGMSLVERDRAASWFADTEDGAQVLVCSEIGSEGRNFQFAHHLVLY
                     DLPQNPDLLEQRIGRLDRIGQNAVIRIHVPYAAGSRRQVLFRWYHEALNALQHTCPAG
                     HQLWLRTRDELQRYLDDPQLPMEAFLADCRELREQLDRQLQQGRDRLLEINSCRPAVA
                     EALIDEALERERELDLFGFMERIYDCYGVNSEIRGQDRWVITPGDNMLTQMPGLPEDG
                     MTVTYKRSAALASEDLHYLTWEHPLVRNAMDMVLSSEFGNTALIAAEYPGIKAGTLLV
                     ECHFLMDFSDDYRSHNERYFPNASIRVLLDENGLDHAARVGPETDSWPVQRVDRDTSI
                     KIVKARQELLAQMLENAELVADTQVPQLIESARAGGQELLGHEIERLVALQQVNRGVR
                     DEEITFFRDRLAQFEAALEHARLRLDAVRVIVAT"
BASE COUNT          583 a         1093 c         1094 g          563 t
ORIGIN      
        1 gccaataaac aatcatactt gcgatcctgg ccgagcggat ttcgagtgag tgcattgtcg
       61 tccaccaggg gcgttaaagt attgctggag cagcgagtgg tgagcaccga gcggctcgcc
      121 ctggtcccga atgctgcagc aacactgcac atcggtggtt ccccggtcgt cgttcaccag
      181 gcgcgcgctg ccgcagtcgg attattccgc ggcatggcgg cgccgaatac ttccgttacg
      241 ccgatggtcg cgggactcgc gcaactgcta taatcgcgcc cgttttacgc ggataacgat
      301 cggcatgcag gaattcgtca tcggccagcg ctggatcagc gctgccgagc ttcacctcgg
      361 cctcggcatg gtaatcgaga tcgagcaccg taccgtgagt atcgttttcc cggccaccgg
      421 ggaaacgcgc atttatgcgc gcgccgacgc gccgctgacg cgggtgcgtt ttcgtgaggg
      481 cgactgggtc gaaaggcagg acggcgaggc gctcagggtt gtgcgggtcg acgaaaacgc
      541 tggcctgatc gtctatcgct gcgaggacga gcacggcggc gcggccgacc tgccggaagg
      601 gcggctgagt aatttcctgc agttcaatca gcccgccgag cgcctgctga acgcacaagt
      661 cgatcgcaac aagtggttca acctgcgctg ccagacccgc gagatcgcca atcggctgca
      721 gcagtcggcc gtctacggcc tggccggcag ccgcaccagc ctgatcgctc accagctgta
      781 catcgcgcac gaagtctcgc gccgctatgc gccgcgtgtg ctgctggccg acgaggttgg
      841 cctcggcaag accatcgagg cgggcctgat cctgcaccag cagttgctgc tcgagcgcgc
      901 gcgacgggtt ttgatcgtgg taccggaatc gctggtgcac cagtggctgg tggagatgct
      961 gcggcgcttc aacctgctgt tcagtatctt cgacgcgcag cgtttcgcgc cggacgacgg
     1021 cgaccaggac ttcgtcgacg tcgccgaaaa cccgttccac gccgagcagc tggtgctgtg
     1081 taccctcgag ttcctgctcg cccacccgca gcatgccgcc gaggcgcgcg ccggggactg
     1141 ggacctgctg gtggtcgacg aggcgcatca cctggagtgg tcgcccgacg cggccagcga
     1201 cggctaccgg ctggtcgagg gcctggcgga aaacaccgcg ggcctgctgt tgctgaccgc
     1261 gacaccggaa caactcggca aggaaagcca cttcgcgcgc ctgcggctgc tggacccgga
     1321 ccgcttcgac agcctcgggc gctttctgca gcaggagcgc ggctacgagg aactggcgcg
     1381 gcaggccgag gccctgctgg aggatcccga gcggctgcgc gaactcgacg acctgctcga
     1441 tcgccacggc accggccggg tgctgttccg caacacgcgc cacgcggtac gcggctttcc
     1501 cgaacgcgag gtgcggccgg tcgagctcga agccgacgcc gacgaagccg cctacgcgac
     1561 gtggctgggt ggattcctgc gcgaataccg ggaccgcaag gtgctggtga tcgccgccgg
     1621 cgcggatacc gcgatcagtg tcaccgagcg gctcaaacag gaattcgggc tgcgcgcgac
     1681 cacctttcac gagggcatga gcctggtcga acgcgatcgc gcggcgagct ggttcgccga
     1741 taccgaggac ggtgcccagg tgctggtgtg ctcggaaatc ggcagcgagg ggcgcaattt
     1801 ccagttcgcg catcacctgg tgctctacga cctgccgcag aacccggacc tgctggagca
     1861 gcgcatcggc cggctcgacc gtatcggcca gaacgcggtg attcgcattc acgtgccgta
     1921 cgcggccggc agccggcggc aggtactgtt tcgctggtac cacgaagcgc tgaacgcgct
     1981 gcagcacacc tgcccggccg gccatcagct gtggctgagg acgcgtgacg aattgcagcg
     2041 ctacctcgac gatccgcagt taccgatgga ggcattcctc gccgactgcc gcgaactgcg
     2101 cgagcagctc gaccgccagt tgcagcaggg gcgcgaccgc ctgctggaaa tcaactcctg
     2161 ccgaccggcc gtggccgagg cgctgatcga cgaggcgctg gagcgcgaac gcgagctcga
     2221 cctgttcggc ttcatggagc gcatctacga ctgttatggg gtcaacagcg agattcgcgg
     2281 ccaggaccgc tgggttatca ctcccgggga taacatgctc acgcagatgc cagggctgcc
     2341 cgaggacggc atgacggtga cctacaagcg cagcgccgcg ctggccagcg aagacctgca
     2401 ctacctgacc tgggagcacc cgttggtgcg caacgcgatg gacatggtgc tgtccagcga
     2461 gttcggcaat accgcgctga tcgcggccga gtatccgggt atcaaggccg gcactttgct
     2521 cgtcgaatgc cattttttga tggatttcag cgatgactat cgcagccaca acgagcgcta
     2581 ttttccgaac gcgagcattc gcgtgttgct cgacgagaac ggcctcgacc acgccgcgcg
     2641 cgtcgggccc gaaaccgact cgtggccggt acagcgagtc gatcgcgata cctcgatcaa
     2701 gatcgtcaag gcgcgccagg agctgctcgc acaaatgctc gaaaatgccg agctggttgc
     2761 cgacactcaa gtaccgcaac tgatcgaatc cgcgcgtgcc ggcggccagg agctgctggg
     2821 ccacgaaatc gagcgactgg tcgcgctgca acaggtcaat cggggcgtgc gcgacgagga
     2881 aatcacgttt ttccgcgacc ggctagcgca gttcgaggcg gcgctcgagc acgcccggtt
     2941 gcggttggac gccgtccgcg tcatcgtcgc tacctagtcc gcggaagcgc tgcggacgcc
     3001 gcaaagcgca ccgtgacgcg ccgtactcgc aaagtgacgc tcaacgtagt ttgcactacg
     3061 cctgcgcgcc cctttgctgc gtgcgacgcg ccacggcgtc tttgcgacgc cctcgctttt
     3121 caatccagtg gacctctcga cagctttgct gcggacgccg caaagcgcac tgtggcggcc
     3181 cgtactccct gcgggccgct caacgtagct tacgctacgc ctgcgcgacc ctgcggtccg
     3241 tgcggacccc cacagcgtct ttgcgacgcc ctcgcttgtc aagccagtgg acctctcggc
     3301 agctttgctg cggacgccgc aaagcgcacc gtg
//
LOCUS       DATCZV010000479         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_145415, whole genome shotgun sequence.
ACCESSION   DATCZV010000479 DATCZV010000000
VERSION     DATCZV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145415"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VIW27_10810"
     CDS             complement(<1..277)
                     /locus_tag="VIW27_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5740207.1"
                     /translation="MYDWNDLRYFLELSRQGKLVRAAARLHVDHTTVSRRITALEKQL
                     DVRLFDKSPSGYQLTDAGLRLLPLAEQIESSSNRLYQDIAGKDALLGG"
     gene            597..2123
                     /locus_tag="VIW27_10815"
     CDS             597..2123
                     /locus_tag="VIW27_10815"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5740208.1"
                     /translation="MSQPAAAVSSSITQLTHFVGGAHVSGTSGRHGDVFNPTLGIKSG
                     EVPLASRAEVENAIAVAEQAFSGWAATSVLQRSRIMARYVQLLYQHQEELAELVSKEH
                     GKVFEDAMGSVLRGIEVCEFACGAPHAQKGEFSDNVARGIDIYSMRKPLGVVAGITPF
                     NFPAMIPLWMAGMALITGNTVVMKPSEKDPSCPLRLAELFIEAGGPAGVFNVVNGDKE
                     AVDTLLEDPRVKAVSFVGSTPIAKYVYATATANGKRCQAMGGAKNHMLILPDADLEDV
                     ANALMGAAYGSAGERCMAISVGVCIGDEVADRLIEILKPRVEALRIGPSLERGLEMGP
                     LVTREHREKVMNYIQMAADEGSRLVVDGRDFVLEGHADGYFLGGSLIDDVKPGMQSYQ
                     EEIFGPVLQIMRVGSFEEGAQLATDHPYGNGTSIFTRNGAAARTFADKVEVGMVGINV
                     PIPVPLAFHSFGGWKSSAFGDHNQYGMEGLRFYTKVKTVTSRWTDDGLDAEFMMPVLK
                     "
     gene            2161..2397
                     /locus_tag="VIW27_10820"
     CDS             2161..2397
                     /locus_tag="VIW27_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740209.1"
                     /translation="MIATLVFFGVAFSLLHLVILWLGLPDLAALLLLTIPVIAYLAYC
                     TQPTRPAMLRSAIRIYTSFAAVVLSAGGLVYLIG"
BASE COUNT          457 a          786 c          760 g          508 t
ORIGIN      
        1 tgccgccgag cagcgcgtcc ttgccagcga tatcctggta caggcgattg gaactggatt
       61 cgatttgttc ggccagcggt aacagccgaa ggccggcgtc ggtcagctgg taaccgctcg
      121 gtgacttgtc gaacaggcgc acgtcgagct gcttttcgag cgcggtgatc cgccggctga
      181 cggtggtgtg atcgacatgc aggcgcgccg ccgcgcgcac cagcttgccc tgccgcgaca
      241 gttcgagaaa gtaacgcaaa tcgttccagt catacatccc gactatccgt catttctgct
      301 gcgcagaaat gcctcctcaa aacgtcatcc ccgcgcaagc ggggatcttg taaagctccg
      361 cgctaattga agaatccccc gcctcagtcc cagtcccagt tccagtccga agagaatcta
      421 tttatcggtg ccacatatta tgaagattcc cgccgggccg caggcggttt gcgcgggaat
      481 ggcaacctga tgtgtatttt tgcacaatgg ttgtgatatt tgcagccttt ctttgcacaa
      541 atatcttgtc tatactgacc gccctgttaa ccgtacccgc ctgttccgga gccgtcatga
      601 gccagccagc cgcagccgtt tcttcatcga ttacccagct gacccacttc gtgggcggcg
      661 cacacgtcag cggcaccagc ggccgccacg gcgatgtctt caacccgacc ctcggcatca
      721 agtccggcga agtgccgctg gcgtcgcgcg ccgaggtcga aaacgcgatc gcggtcgccg
      781 agcaggcatt ttccggctgg gccgcgacct ccgtattgca gcgttcgcgg atcatggcgc
      841 gctacgtgca gctgctctac cagcaccagg aagaactcgc cgaactggtc tccaaagagc
      901 acggcaaggt gttcgaggac gcgatgggat cggtgctacg cggcatcgag gtctgcgagt
      961 tcgcctgcgg cgcgccgcac gcgcaaaagg gcgagttttc cgacaatgtc gcgcgcggca
     1021 tcgatatcta ctcgatgcgc aagccgctcg gcgtggtcgc cggcatcacg ccgttcaact
     1081 ttccggcgat gattccgctg tggatggccg gcatggcgct gatcaccggc aacacggtag
     1141 tcatgaaacc ttccgaaaaa gacccttcat gcccgctgcg actcgccgaa ctgttcatcg
     1201 aggcgggcgg tcctgccggg gttttcaatg tcgtcaacgg cgacaaggaa gccgtcgaca
     1261 cgctgctcga ggacccgcgc gtcaaggcgg tcagcttcgt cggctcgacg ccgatcgcga
     1321 agtacgtcta cgccacggcg accgccaacg gcaagcgttg ccaggcgatg ggcggcgcca
     1381 agaaccacat gctgatcctg cccgacgccg atctcgagga cgtcgccaac gcgctgatgg
     1441 gcgcggctta cggttcggcc ggcgaacgct gcatggcgat ctcggtcggt gtctgcatcg
     1501 gtgacgaggt tgccgaccgc ctgatcgaaa ttctcaagcc gcgggtcgaa gcgctgcgca
     1561 tcggtccgag cctcgagcgg ggcctcgaaa tggggccgct ggtgaccagg gagcaccgcg
     1621 agaaagtcat gaactacatt cagatggccg cggacgaggg ttcgcgcctg gtcgtcgatg
     1681 gccgcgattt cgtgctcgag ggtcacgcag acgggtactt tctcggcggc tcgctgatcg
     1741 acgacgtcaa acccggcatg caatcctacc aggaagaaat cttcggtccg gtgttacaaa
     1801 tcatgcgcgt cggctcgttc gaagaaggcg cccagcttgc caccgatcac ccttacggta
     1861 acggcacctc gatttttacc cgcaacggcg ccgcggcgcg taccttcgcc gacaaggtcg
     1921 aagtcggcat ggtcggcatc aacgtgccga ttccggtgcc gctcgctttt cacagtttcg
     1981 gcggctggaa atcgtccgcg ttcggcgacc acaaccagta cggcatggaa gggctgcgtt
     2041 tttataccaa ggtgaagacc gtgacctcgc gctggaccga cgacggtctc gatgccgagt
     2101 tcatgatgcc ggtgctgaaa taaaccagtc ggccgccggc cgttgaaacc gatcgcttca
     2161 ttgatcgcca ccctggtttt tttcggggtg gcgttttcgc tgctgcacct cgttatcctg
     2221 tggttggggc tgccggacct ggcggcgctg ctgctgttga cgatcccggt gatcgcttac
     2281 ctcgcttact gcacccagcc gacgcgcccc gcgatgctac gctcggcgat ccgcatctac
     2341 accagtttcg cggcggtcgt gctgagcgcc ggtggactgg tttacctgat cggctgagcg
     2401 cgcgcgaaac tcgcggcgat tggcgccggc atacaacgtg aaatcgcgct gtcgcggcga
     2461 aagttttggt tgaaaatagt tctgtccccc ctcttttctt tgcgatgacc t
//
LOCUS       DATCZV010000480         4492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_410268, whole genome shotgun sequence.
ACCESSION   DATCZV010000480 DATCZV010000000
VERSION     DATCZV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410268"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VIW27_10825"
     CDS             <1..298
                     /locus_tag="VIW27_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5740210.1"
                     /translation="CRRQGVPIMAYSPLDQGGSLLQSPVLNEVASRHSASAAQIALAW
                     LLQRPDTVIIPKSARPQRILENLQALEIELGADDRQALEQAFPPPDGPVRLGMR"
     gene            complement(334..1623)
                     /locus_tag="VIW27_10830"
     CDS             complement(334..1623)
                     /locus_tag="VIW27_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5740211.1"
                     /translation="MTSCSGLLSKITRAFLLQAGMITLAALLGVYLAKIVIEETLVKN
                     AILEKAEYFWKHYLGDRDFGLPDTKNLTGYFDPQLLPPVIRDALPAALGFYEFSDLSN
                     RLVLYLDRRDGETLRLVYYRGQVDALVLYYGLLPLLVVLTRLHLALWFAYRSSRRTIS
                     PITRLARHIDSIDLGKEGGSLRLDDPGLQGDDEIQVLADAIADLGERVDAFIARERHF
                     TRNASHEFRSPLTVINTAADMLLLDAGLSAASREVLYKIKRAVADMESLTEVFLMLAR
                     EDAGQLTRRPVELDKVVREQIERAEFICGSARPRIHLRTRARAEVDSSETVLAVLIGN
                     LIRNAVLYADDGEIDIEVDSDRLTISDSGPVIPEHSLDRLFEPFHRAGCENASGHGIG
                     LTIVKRLCDRFGWQIEVASGARGGASFRLDFAPRAAP"
     gene            complement(1616..1861)
                     /locus_tag="VIW27_10835"
     CDS             complement(1616..1861)
                     /locus_tag="VIW27_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740212.1"
                     /translation="MKTNPHRSRTWLASCVIAATLIAAGCGSSDNDHNDAPTSQLRVI
                     PASHDAPPVNLKLNGSVALSGLDYAASTGFVDVANDD"
     gene            2018..2878
                     /locus_tag="VIW27_10840"
     CDS             2018..2878
                     /locus_tag="VIW27_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY5740213.1"
                     /translation="MNTQNRRCRLREIFNGERCIHPGSVFDPMSARAAQDLGFEAGMF
                     AGSIASYTVLGAPDIIVLTLSEFAAQAQRICRAAELPLIVDADHGYGNALNVMRTVEE
                     LETAGISCLTIEDTLLPKRFGAEGTEFLSIEEGVGKMKAALAGRQDPDLVLLGRTSAV
                     GPNGVDDGIARASAYLDAGVDGIMFVGLKTRAELEAIAAALDAPLMIGGAGAELQNAD
                     YLAGCGVRVALQGHQPFMAAVQAMYATLQALREGVAPAALEGVASAELMKQLTRDEQY
                     ADWMDEFLGG"
     gene            complement(2900..4477)
                     /gene="gshA"
                     /locus_tag="VIW27_10845"
     CDS             complement(2900..4477)
                     /gene="gshA"
                     /locus_tag="VIW27_10845"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY5740214.1"
                     /translation="MKAEFEKRVAHLLGSDIRQVLQNRKVGLEKESLRVAVDGGIAQT
                     PHPRALGSALTHPNITTDYSEALIEIVTPPFNHFCETLEFLDDTHRFVYSKLDNEILW
                     ATSMPCVVEGDASIPIAHYGSSNAAQMKSAYRRGLGHRYGRMMQAIAGVHFNYSYPPE
                     FWRLYGEEFGDRRELQAFASEAYMGMIRNLQRMGWLVPYLFGASPAICASFLGASPTS
                     LDKWQQYSYYGPYATSLRMGDIGYQNSKEGEAGINVNYNNLEAYIDSLTYAISTPYPE
                     YQKIAMVDNGEWQQLNANILQIENEYYSSVRPKQILDGEEKPTLALARRGIAYLELRS
                     LDVNAYDPLGISEDQMRFLESFLLYSLMTPSEPYDDDEILSIRENINAVAVRGRDPAL
                     RLRRNGDEVRLRDWANEIFEQMAFSCELLDKGAPNSCYVDGLGEQHAKVADADLTPSA
                     RTLADMAEHKEEFYHFALRKSREHQQRFAERPLEAEKLDSFEQQARASLVAQQRIEET
                     DDIPFATFLDNYFAQAL"
BASE COUNT          804 a         1351 c         1489 g          848 t
ORIGIN      
        1 gtgccggcgg cagggagtgc cgatcatggc ttactcgccg ctcgaccagg gcggctcgct
       61 gctgcagtcg ccggtgctca acgaggtggc gtcgcggcat tcggcaagcg cggcgcaaat
      121 cgcgctcgcc tggctgctac agcgccccga taccgtgatc attcccaagt cggcacgccc
      181 acagcgcata ctcgaaaacc tgcaggcgct ggaaatcgaa ctcggcgcgg acgaccggca
      241 ggcgctcgag caggcctttc cgccgcccga cggcccggtg cgcctcggca tgcgctgacc
      301 gcgcgtttgc cgcgcggccg cgacggggtt cgctcagggc gccgcgcgcg gcgcgaagtc
      361 aagcctgaag ctggcaccgc cccgcgcacc gctcgcgacc tcgatttgcc agccgaagcg
      421 atcgcacagg cgcttgacga tggtcaggcc gatgccgtgg ccagacgcat tttcgcagcc
      481 ggcacgatga aagggttcga acaggcggtc gaggctgtgc tcggggatta ccggcccact
      541 gtcggaaatc gtcagccggt cgctgtcgac ttcgatgtcg atctcgccgt catcggcgta
      601 gagcaccgcg ttgcgaatca ggttgccgat cagcaccgcc agcaccgtct ccgatgagtc
      661 gacctcggca cgggcccggg ttcgaaggtg gatgcgcggc cgcgcgctgc cgcaaatgaa
      721 ctcggcgcgc tcgatctgct cgcggaccac cttgtcgagt tcgaccggtc gccgcgtcag
      781 ctggccggca tcctcgcgcg ccagcatcag gaagacctcg gtcaggcttt ccatgtcggc
      841 gaccgcgcgc ttgatcttgt acagtacttc ccgcgaagcc gcggacagcc cagcatcgag
      901 cagcagcatg tcggcggcgg tgttgatcac cgtcaacggg ctcctgaact cgtggctggc
      961 attgcgcgtg aaatggcgtt cccgggcgat gaacgcatcg acacgctcgc cgaggtcggc
     1021 gatcgcgtcg gccagcacct ggatttcgtc gtcaccttgc agtcccgggt cgtcgagccg
     1081 tagcgagccg ccctccttgc cgagatcgat gctgtcgata tgcctggcga gccgcgtgat
     1141 cggcgagatg gtgcgccggc tggaacggta ggcgaaccac agggccaggt gcagccgggt
     1201 cagcaccacg agcagcggta acaggccgta gtagagcacc agcgcatcga cctggccgcg
     1261 ataataaacc aggcgcaggg tttcgccgtc gcggcggtcg aggtacagca ccagccggtt
     1321 gctgaggtct gaaaattcgt aaaagccgag ggccgccggc agagcgtcgc ggatcaccgg
     1381 cggcagcagc tgcgggtcga agtaaccggt caggttcttg gtatccggca ggccgaaatc
     1441 gcggtcgccg aggtagtgct tccagaaata ctcggctttc tcgagaatcg cgttcttgac
     1501 cagggtttcc tcgatcacga tcttcgccag gtaaacgccg aggagggcgg ccagcgtaat
     1561 catcccggcc tgcagcagga aggcacgggt aatcttgctc agcaggccgc tgcaactagt
     1621 catcgttggc gacgtcgacg aaaccggtgc ttgcagcata gtcgaggccg ctcaacgcga
     1681 cgctgccgtt caacttcaaa ttgaccggcg gtgcgtcgtg ggaagcggga atcactcgca
     1741 gctgagaggt cggggcgtcg ttatggtcgt tgtcggaact gccgcaaccg gcggcaatca
     1801 gggtggcagc gattacgcag ctggcgagcc aggttcgcga gcgatggggg ttggtcttca
     1861 tcggggtctc caggttgtag aaaacgccgc aactctgcga gcggccgtgt gaagaaaccg
     1921 tgaagacgga atgaaggtgt tgtgaaagat cggcctgcag cagtgcgccg cgtgatgtta
     1981 agatcggtac cagaacaaac cggcaagcga ggaatccatg aatacccaga accggcggtg
     2041 caggctgcgc gagattttca acggtgagcg ctgtatccac ccgggctcgg tgttcgatcc
     2101 gatgtctgcg cgtgccgccc aggacctcgg cttcgaggcc ggcatgttcg ccggctcgat
     2161 cgcctcctac acggtgctcg gcgcccccga tatcatcgtg ctgacgctaa gcgaatttgc
     2221 cgcccaggcg caacgcattt gccgtgccgc ggaattgccg ttgatcgtcg atgccgacca
     2281 cggctatggc aacgcgctga acgtcatgcg caccgtcgag gaactggaaa ccgcgggcat
     2341 ctcctgcctg acgatcgagg atacgctgtt gccgaaacgc ttcggcgccg agggcaccga
     2401 gtttttatcg atcgaggaag gcgtgggcaa gatgaaagcc gcgctcgccg gtcgccagga
     2461 tccggacctg gtgctgctcg gacgcaccag cgcggtcggg ccgaacggcg tcgacgacgg
     2521 catcgcgcgc gccagcgcct acctcgatgc cggtgtcgac ggcatcatgt tcgtcggcct
     2581 gaaaacgcgc gccgagctgg aagcgattgc cgccgcgctc gatgcgccat tgatgatcgg
     2641 cggtgcgggc gcggaactgc agaatgccga ttacctggct ggctgcggcg tgcgcgtcgc
     2701 gctgcagggc caccagccgt tcatggcggc ggtgcaggcg atgtacgcta cgctgcaggc
     2761 gctgcgcgag ggcgtcgcac cggcggcact cgagggagtg gcgtcggcgg agttgatgaa
     2821 gcagctgacc cgcgacgaac agtacgctga ctggatggac gaattcctcg gcggctgagc
     2881 agcggggcct gtcgataatt tacagggctt gcgcgaagta gttgtcgaga aaggtcgcga
     2941 atggaatgtc gtcggtttcc tcgatgcgct gctgcgcgac cagtgatgcg cgcgcctgct
     3001 gttcgaaact gtcgagtttt tcagcctcca gcggccgttc cgcgaaccgt tgctggtgct
     3061 ctcgcgactt gcgcagcgcg aagtggtaaa actcctcctt gtgctcggcc atgtcggcga
     3121 gcgtgcgtgc cgacggcgtc agatcggcat ccgcgacctt ggcgtgttgt tcgccgagcc
     3181 cgtcgacgta acaggaattc ggtgcgccct tgtccagcaa ttcgcaggaa aacgccatct
     3241 gttcgaaaat ttcattggcc cagtcgcgta acctgacttc gtcgccgtta cgcctgagac
     3301 gcagggcggg gtcgcgaccg cgcaccgcga cggcattgat attttcgcgg atcgagagga
     3361 tttcgtcgtc gtcgtagggc tcgctcggcg tcatcaggct gtataacagg aaggattcga
     3421 gaaagcgcat ctggtcctcg ctgatgccca gcggatcgta ggcgttgacg tcgagcgagc
     3481 gcagttcgag gtaagcgatg ccgcgccgcg ccagcgccag ggtcggcttt tcctcgccgt
     3541 ccagaatctg cttcggtcgt acgctcgagt aatactcgtt ttcgatctgc aggatattgg
     3601 cgttcagctg ctgccattcg ccgttatcga ccatggcgat cttctggtac tcggggtagg
     3661 gcgtggagat cgcataagtc aggctgtcga tgtaagcctc gaggttgttg taattgacgt
     3721 tgataccggc ttcgccttcc ttgctgttct ggtagccgat gtcgcccatg cgcagcgagg
     3781 tcgcgtaggg tccgtagtag gaatattgct gccacttgtc gagcgatgtc ggcgaagcgc
     3841 cgagaaacga ggcgcaaatc gccggcgaag cgccgaacag gtagggcacc agccagccca
     3901 tgcgctgcag gttgcgtatc attcccatgt aggcttctga agcgaaggcc tgcagctcgc
     3961 gccgatcgcc gaattcttcg ccgtacaggc gccagaattc cggcggatag gaatagttga
     4021 aatgcacgcc cgcgatggcc tgcatcatac gcccgtatcg atgcccgaga ccacggcggt
     4081 aggcggattt catctgtgcc gcgttggagc tgccgtagtg cgcgatcggg atactggcat
     4141 cgccctcgac cacgcagggc atgctggtgg cccacagaat ctcgttgtcg agcttgctgt
     4201 agacgaagcg atgggtatcg tcgagaaact cgagtgtttc gcaaaaatgg ttgaacggcg
     4261 gcgtgacgat ctcgatcagc gcctcggaat aatccgtggt gatgttggga tgggtcaacg
     4321 ccgaaccgag cgctcgcggg tgcggggtct gcgcgatacc gccgtcgacg gcgacccgca
     4381 ggctttcctt ttcgaggccc accttgcggt tttgcaatac ttgcctgatg tcgctgccga
     4441 gcaggtgcgc gacgcgtttt tcaaattctg ctttcaactt cgaatccagc ct
//
LOCUS       DATCZV010000481         2606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_571031, whole genome shotgun sequence.
ACCESSION   DATCZV010000481 DATCZV010000000
VERSION     DATCZV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571031"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VIW27_10850"
     CDS             complement(<1..226)
                     /locus_tag="VIW27_10850"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642635.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5740215.1"
                     /translation="MNKLIIKGGVPLSGQVRISGSKNAVLPILAGTLLADRPVIVRNV
                     PHLHDVTTTMELLGRMGVLLTIGEKMSIEID"
     gene            complement(350..937)
                     /locus_tag="VIW27_10855"
     CDS             complement(350..937)
                     /locus_tag="VIW27_10855"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740216.1"
                     /translation="MNKICSLILLVLALPAQAATVPENLIRETSDRVIADIRVNRETY
                     RQEPGKIYQLVDTVVLPHFDFSAMTDLALGNYRDQVSAAQRPTIVNEFRQLLVRTYSS
                     ALLEYTDQELTYLPMEGSIEDGQVVVRTEIAQAGGFPIPIDYSLRRGDDGWKVFDISV
                     DQVSLVTNYRSSFARAIKKDGVDGLIKTLQERNQQ"
     gene            complement(934..2304)
                     /locus_tag="VIW27_10860"
     CDS             complement(934..2304)
                     /locus_tag="VIW27_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5740217.1"
                     /translation="MKTYVSRVFWAWLFIAFSSGPQAQERLGIEQAIELALAHDPRID
                     EKQAFVRKAQGLLQEAEGSEGFRYSVDSFLALSTGVDGGFYEGGQNSCSNNCEPRDDL
                     YDLDDGLSLWAGLTFSIVKPLATFGRLENYQEAAQRNIAIKQQDVNLQRDEVALQVVK
                     AYYGYLTARDSRLLLEDTRNRLEAALDLVNDWLEDGSGKVSQSDKYALESGLGLIDNF
                     MAEATGLEQIAMAGLRLLTGLEDETIELADRRIKPVDLPTQSLEEWIELALVNRAEFK
                     QVEAGLAARRALVEATRAGKKPIVFAGIAGSLAYSPDRDRLDNPHIYDPFNHYAMSPL
                     IGMRWQWEQGALPGRVAQAQADLDALVERASFARIGIPFQVQEQYFRMQAKHKSIDSM
                     RSSTRAARRWMIASYSDFEAGLEEADDVINALQVYVLAYAEYLRAVNDFNYLVSKLKS
                     VSGVFQ"
BASE COUNT          476 a          813 c          788 g          529 t
ORIGIN      
        1 gatcgatctc gatactcatt ttctcgccga tcgtcagcag cactcccatg cggccgagca
       61 attccatggt ggtggtgacg tcgtgcaggt gcggcacgtt gcgcacgatg accggccggt
      121 cggccagcag cgtgccggcg agtatcggca gcaccgcatt cttggagccg gagattcgta
      181 cctgcccgga cagcggtacg ccgcccttga taatcaattt attcatgcgc tggcaatcgg
      241 ttaggggcgc tcattctaac agaagcgagg cccgagtata gccgtttgcg cggcccccgg
      301 attcgccgga gacagggccg cgcgacgggt ccggcacggc atcccgcggc tattgctgat
      361 tgcgttcctg cagggtcttg atcaagccgt cgacgccgtc cttcttgatc gcccgcgcga
      421 agctcgagcg gtaattggtt accaggctga cctggtcgac actgatgtcg aataccttcc
      481 agccatcgtc gccgcgacgc aggctgtaat cgatcggaat cgggaaaccg ccggcctggg
      541 cgatctcggt cctgaccacg acctggccat cctcgatact gccttccatc ggcaggtagg
      601 taagttcctg gtcggtgtac tcgagcagcg ccgagctata ggtgcgcacc agcaactggc
      661 ggaactcgtt gacgatggtc ggtcgctgcg cggcgctgac ctggtcccgg taattgccga
      721 gcgcgaggtc ggtcatcgcc gagaaatcga aatgcggcaa caccacggta tccaccagct
      781 ggtagatctt gccaggctcc tgccggtagg tttcgcggtt gaccctgata tcggcgatca
      841 ccctgtcgga ggtctcgcga atcaggtttt ccggaaccgt cgcggcctgc gccggcaacg
      901 ccaggaccaa caggatcagg ctgcaaattt tgttcactga aatactccac tgacgctttt
      961 tagcttcgac accagataat tgaaatcgtt gaccgcgcgc aggtactcgg cataggcgag
     1021 cacgtagacc tgcagagcgt tgatcacgtc gtcggcctcc tcgagcccgg cttcgaaatc
     1081 ggaataggag gcgatcatcc agcggcgcgc ggcgcgcgtg ctgctgcgca tcgagtcgat
     1141 cgacttgtgc ttggcctgca ttctgaaata ctgctcctgc acctggaacg gaatgccgat
     1201 gcgcgcgaac gacgcccgtt ctaccagcgc gtcgagatcg gcctgggcct gcgccacccg
     1261 gcccggcagc gctccctgct cccactgcca gcgcatgccg atcagcggcg acatcgcgta
     1321 gtgattgaac gggtcgtaga tatgcgggtt gtcgagacgg tcgcggtccg gcgagtaagc
     1381 cagcgaaccc gcgatgccgg caaagacgat cggcttcttg cccgctcgcg tggcctcgac
     1441 cagcgcgcgg cgcgcggcga ggccggcttc gacctgcttg aattcggcgc gattgaccag
     1501 cgccagctcg atccattcct cgaggctttg cgtcggcagg tcgacgggct tgatacgccg
     1561 gtcggcgagt tcgatggttt cgtcctcgag cccggtcagc agtctgagac cggccatcgc
     1621 gatttgctcc agaccggtgg cctcggccat gaaattgtcg atcaggccca gtcccgactc
     1681 gagcgcatac ttgtccgact ggctgacctt gccggagccg tcctcgagcc agtcgttgac
     1741 caggtcgagc gccgcctcca ggcgattgcg agtatcttcg agcagcaatc ggctgtcgcg
     1801 agcggtcagg tacccgtaat aggctttcac cacctgcaag gcgacctcgt cgcgctgcag
     1861 gttgacgtcc tgctgcttga tggcgatgtt tctctgcgcg gcttcctgat aattttcgag
     1921 acgcccgaag gtcgccagcg gcttgacgat actgaaggtc aggccggccc acagcgacag
     1981 cccgtcgtcg agatcgtaga gatcgtcgcg cggctcgcag ttgttcgagc aactgttttg
     2041 gccgccctcg taaaaaccgc cgtcgacgcc ggtcgacagg gccaggaaac tgtccaccga
     2101 gtaacgaaac ccctcggatc cctcggcttc ctgcagcagg ccctgggcct tgcgcacgaa
     2161 ggcctgcttt tcgtcgattc gcggatcgtg ggccagcgcg agttcgatgg cctgctcgat
     2221 accgagccgc tcctgggcct gcgggccgct gctgaacgcg ataaacagcc aggcccagaa
     2281 tacccttgat acatacgtct tcatggcgat gaatgatccg tagctaagct gaacacgggc
     2341 tgactccgga cgaccggcac agcgcattcg gcagagtcta tcggttaacc ggctggttgt
     2401 gaacggcgcc cgccgggaat cgcggcattt attattttac tggtaaattt gtcacataat
     2461 cgttagtgtt cgcaagctcc gtaaattgaa tccccatttt ccatgtcgcc aacaccgctt
     2521 accgacaggc acagcttcga ctacgacgaa ttaatcgatt gcggcaaggg caagctgttc
     2581 gggcccggca atgccctgtt gccgct
//
LOCUS       DATCZV010000482         5910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_199214, whole genome shotgun sequence.
ACCESSION   DATCZV010000482 DATCZV010000000
VERSION     DATCZV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199214"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VIW27_10865"
     CDS             <1..277
                     /locus_tag="VIW27_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HEY5740218.1"
                     /translation="MGVGFLAVLVLLGALREIIGFGTLFANAELMFGEGARGLTIAIN
                     PDYPGFLLAILPPGAFFGLAILIVIKNWFDLRPATRPAGIPAAAESA"
     gene            274..909
                     /gene="nth"
                     /locus_tag="VIW27_10870"
     CDS             274..909
                     /gene="nth"
                     /locus_tag="VIW27_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY5740219.1"
                     /translation="MNPQKRQQIFERLRAHIESPRSELEYRSHFELLIAVLLSAQATD
                     VSVNKATARLYPMANTPEAMLALGEEGIREYIKTIGLYNAKAKNIIATSKILIEQYGS
                     EVPADRAVLESLPGVGRKTANVILNVAFGQPTIAVDTHIFRVSNRTRFAPGRNVLEVE
                     KKLLRFVPDDFKLDAHHYLILHGRYTCVARKPRCGSCVIADLCEYKAKNLD"
     gene            1167..1583
                     /locus_tag="VIW27_10875"
     CDS             1167..1583
                     /locus_tag="VIW27_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY5740220.1"
                     /translation="MFQSRDEIRRVYAKVWQKMQQGQLLEPMEALIAEVIESHPEYHP
                     LLDDQANIEQQEFIPEQGQTNPFLHMGMHIALREQAGSDRPPGIRPVYDRICAARGRH
                     QAEHAMMECLGQSLWQAQRDNRPPDETAYLECLKKL"
     gene            1927..3531
                     /locus_tag="VIW27_10880"
     CDS             1927..3531
                     /locus_tag="VIW27_10880"
                     /EC_number="4.1.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HEY5740221.1"
                     /translation="MSRKQDLNVGQATIELLEQYGVDTLFGIPGVHTLEFCRGLNSSG
                     IRHVQARHEQGAGFMADGYARVSGKPGVALLISGPGVTNALTAIGQAYADSIPLLVLS
                     SDAASHTLGKGWGCLHEVPGLTDATAPLTAFSATAMNPEEVPELIARAFAVFAGERPR
                     PVHIAIPIDVLEMPAGAGWQAVEPPERPMPTAKKIARACDLLRAALQPMILIGGGAAL
                     AGEEITALAERLGAAVISSTAGKGIIDDSHPLSLPGGRVRGEVQDYLNQADVVLVVGS
                     ELSEVDSFAAPLEINGKIIRIDLDARKMHDLYPVEVAIIGGARASCAAILRELEDIEP
                     RSASADEVAEIRRRIGDNLEGSESRHRRVLEVLRASLPAATRVMADVCQLSYSGAFAF
                     DVPRPRHWNYPAGYCTLGSALPCAIGARLALPDAPVVAIAGDGGFMFTVQELVTAAEL
                     ELPLPIILWNNEGLKQIQDDMKARGIELVGVTGINPDFVALAEACRCRALKVDSAEGL
                     SAAIDAAWKADRPTLIEVNEWDPWLK"
     gene            3586..5307
                     /locus_tag="VIW27_10885"
     CDS             3586..5307
                     /locus_tag="VIW27_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5740222.1"
                     /translation="MIARPRKTLQRLAGVSLIGLAIGLIAAFATTAFVEGIRFLNRLL
                     LVSPASRLGLEPVELFVVTTLSLTLGGLAVGLILRYGVDSGKPAGPVDTIYAVQLHER
                     LPSPRSGLFSTLAAATSLGCGASVGQYAPLVYLGTLVGQVTNRLPLGFAKVRSIAISC
                     GVAAAISTAFNTPIAALIFTHEVILRHYSPRMFTAVAVASASGYLVDNVIFERTPLIQ
                     FSMFGDFRGVELPLFALLGVACGLLAVLFMKMLDRVRQLAAQTALPAPLKPMLAGFLL
                     SLVAWQVPEVLGAGEEVFRTAVHGYLFSSGDLLVILVAKILVTAMCIGFGFAGGVFFP
                     SLLIGALFGALFAHLVPAQLLDGYSGLSTYAVCGTAALMGPVIGAPMTALVLVFEFTR
                     SYEITVAAMLAIVFANLVSHHWHARSQWDFELGARGIDLSLGRERAYLRHQKVVRLAI
                     DALPVLASAHTVGGLREQLRTATTKTAVIVDDDGNYLGQVSRTDLGKLQGSETVASLD
                     FENGKLFDETTSIWDAMQAMRNYIGEAIPVVDSRTGRYLGAVPESAVINAYLDAAQEL
                     RREEHEV"
     gene            5311..>5910
                     /gene="nadC"
                     /locus_tag="VIW27_10890"
     CDS             5311..>5910
                     /gene="nadC"
                     /locus_tag="VIW27_10890"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY5740223.1"
                     /translation="MEFHQLIVNQAANALDEDVGSGDISAELIDASARLDTELLVRED
                     AVLCGCPWFDEVFRQCDDTIDIEWLARDADAITAGSVIARISGAARPLLTAERSALNF
                     LQTLSATATVTRRYADILRGTGCRILDTRKTIPQLRHAQKYAVVCGGGTNHRIGLFDA
                     YLVKENHLAACGGIAPAAARARELHPDRLLEIEVETLEQL"
BASE COUNT         1084 a         1876 c         1843 g         1107 t
ORIGIN      
        1 gatgggtgtc ggctttctcg cggtcctggt gctgttaggc gcgctgcgcg aaatcatcgg
       61 tttcggcacg ctgttcgcca acgccgaact gatgttcggc gagggcgcgc gcggcctgac
      121 gattgccatc aaccccgatt atcccggctt cctgctggcg attctgccgc ccggcgcctt
      181 tttcgggctg gcgatcctga tcgtcatcaa gaactggttc gatctgcgac ccgcgacccg
      241 ccccgccgga ataccggccg cagccgagag cgcatgaatc cacaaaagcg acagcaaata
      301 ttcgaacgcc tgcgtgcgca catcgagagt ccgcgatccg agctagaata ccgctcgcac
      361 ttcgaactgt tgatcgcggt gttgctgtcg gcacaggcca ccgacgtcag cgtcaacaag
      421 gcgaccgcgc gactctaccc gatggccaac acacccgagg cgatgctcgc gctcggcgaa
      481 gaaggcatca gggaatacat caagaccatc ggtctttaca acgccaaggc gaaaaacatc
      541 atcgccacca gcaagatact gatcgaacaa tatggttccg aggttcccgc ggaccgcgcc
      601 gtgctcgaat ccctgcccgg cgtcggccgc aagaccgcca acgtgatctt gaacgtcgcc
      661 ttcgggcagc cgacgattgc cgtcgatacg catattttcc gggtgtcgaa tcgcacccgt
      721 ttcgctcccg gtagaaacgt gctcgaggtc gagaaaaaac tgctgcgttt cgtgccggac
      781 gacttcaagc tcgacgccca ccattacctg atcctgcacg gacgctatac ctgcgtcgcc
      841 cgtaagccgc gctgcggcag ttgcgtgatc gccgaccttt gcgaatacaa agccaagaac
      901 ctggattagc ctgaatgttg caccagtcgg tctcgagcct aactcaaaaa aacaaattta
      961 atgatgaaaa tttttcgaat tagaatgttt catctccagg gcataatcgg cccaggacct
     1021 attccggcgc taactggcct atttacgtct gcgcttgggc ttccttcgag ccataggtgt
     1081 acctatggct ctcagcccag cccggcgccc agaggcaact gtcccgtgtt agcgtacgga
     1141 atactggtac aacattcagg ctagccatgt tccagtcccg agatgaaatt cgccgggttt
     1201 acgcgaaggt atggcagaag atgcaacagg gacaactgct cgagccgatg gaagcgctga
     1261 tcgccgaggt catcgagtcg cacccggaat atcacccgct gctcgatgac caggcaaata
     1321 tcgaacagca ggaattcatc cccgagcagg gccaaacgaa tccgtttctg cacatgggca
     1381 tgcatatcgc gctgcgcgag caggccggca gcgaccggcc gccgggtatc aggccggtat
     1441 acgatcgaat ctgcgcggcg cgcggacggc accaggcgga gcacgcgatg atggaatgcc
     1501 tggggcaatc gctgtggcag gcgcagcgcg acaaccgccc gcccgacgag acggcttacc
     1561 tcgaatgcct gaaaaagttg tgagctgccg atttacctgt ccctgaatcg ccgctaagct
     1621 agcctgcata ttacctcagc cgaatgagtg gctttcgatt ggttatcgaa tcagggatac
     1681 ctatagcggt ttgtaactgg cggttccggt tacgaactgt agccggcatt cattctgtta
     1741 ccgcgccagc cgctccggtt gccgcatggc ctggtcgtcc ttcatgccat ggctatggct
     1801 atctctgcag accggcgccc tgcgacagcc atcccgcgca ggtaaccgga gggctcatgc
     1861 attacgcagg ctgaaaagcg gattggcgcg cggcaaccgc catcaccagc acctcgagaa
     1921 acagctatga gcagaaaaca ggatttgaac gtcggccagg ccaccatcga gttactcgag
     1981 caatacggcg tggatacgct gttcggcatt cccggcgtac atacgctgga attctgccgc
     2041 ggcctcaata gcagcggcat tcgtcatgta caggcgcgcc atgagcaggg cgccggtttc
     2101 atggccgacg gctacgcgcg cgtcagcggc aaacccggtg tcgcattgct catttccggg
     2161 cccggtgtca ccaatgcgct gaccgccatc ggccaggcct atgccgacag cattccgctg
     2221 ctggtgctgt ccagcgacgc cgccagccac accctcggca agggctgggg ctgcctgcac
     2281 gaggttcccg gtctcaccga tgccaccgcg ccactgaccg ccttctcggc caccgcgatg
     2341 aacccggagg aggtgccgga gttgatcgcg cgggccttcg cggtgtttgc cggcgagcgc
     2401 ccgcggccgg tgcacatcgc gattccgatc gacgtactgg aaatgccggc aggcgccggc
     2461 tggcaggccg tcgagccgcc cgagcggccg atgccgacgg caaaaaagat cgctcgcgcc
     2521 tgcgatttgc tgcgcgctgc gttacagccg atgatcctga tcggtggcgg cgcggcgctc
     2581 gccggcgagg aaatcaccgc gctcgccgaa agactcggcg cggcagtgat ttccagtacc
     2641 gcgggcaagg gcatcatcga cgattcgcac ccgttgtcgc tgccgggcgg ccgagtgcgc
     2701 ggcgaagtgc aggattacct gaaccaggcc gacgtggtgc tggtggtggg cagcgaattg
     2761 tccgaggtcg acagtttcgc ggcaccgctg gaaatcaacg gcaagatcat tcgtatcgat
     2821 ctcgatgccc gcaagatgca cgacctttac ccggtcgagg tggcgatcat cggtggcgcc
     2881 agggccagct gcgcggcgat cctgcgcgaa ctagaggata tcgagccgcg gtcggccagc
     2941 gccgacgagg ttgccgagat ccgccgacgc attggcgata acctcgaggg cagcgagtct
     3001 cgccaccggc gggtcctcga ggtattgcgc gcttccttgc cggccgcaac ccgggtgatg
     3061 gccgacgtct gccagctgtc ctacagtggc gccttcgcgt tcgacgtgcc gcggccgcgg
     3121 cactggaatt acccggccgg gtactgcacg ctgggctcgg cattaccctg cgcgatcggc
     3181 gcgcggctgg cgttgccgga cgcgccggtg gtcgcgatcg ccggcgacgg cgggttcatg
     3241 ttcacggtgc aggaactggt gaccgcggcc gaactcgaat tgccgctacc gatcatcctg
     3301 tggaacaacg aagggctgaa gcaaatccag gacgacatga aggcccgcgg tatcgagctg
     3361 gtcggcgtaa ccggtatcaa tcccgacttc gtcgcactgg cggaagcctg ccgctgccgc
     3421 gcgctgaagg tcgacagcgc cgagggtctc agcgcggcga tcgacgcggc ctggaaggcc
     3481 gatcgaccga cgctaatcga ggtcaacgag tgggatccgt ggctaaaatg aaccgacgcc
     3541 tgtcggcacc agccgtcgag gcggcttcga gggtgtgatt cgcggatgat agccagaccc
     3601 cgcaaaaccc tgcagcgcct cgcaggcgtt tcgctgatcg gactggcgat cgggctgatc
     3661 gcggcctttg ccaccaccgc gttcgtcgag ggtatacgct tcctgaatcg gctgttgctg
     3721 gtatcaccgg ccagccgcct gggcctcgag ccagtcgagt tattcgttgt caccacgttg
     3781 tcgctgacgc tgggcgggct ggcggtgggt ctgatactgc gctatggcgt cgattccggc
     3841 aaaccggccg gcccggtaga taccatttac gcggtccagt tgcacgaacg cctgccgtcg
     3901 ccgcgctcgg ggttgttttc cacgctggcg gcggcgacat cgctgggttg tggtgcttcc
     3961 gtcggacaat acgcgccgct ggtttatctc ggcacgctgg tcggccaggt gaccaaccgc
     4021 ctgccgctcg gtttcgccaa ggtgcgctcg atcgcgattt cctgcggggt cgccgcggcc
     4081 atttcgaccg cgttcaatac gccgatcgcg gcgctgatct tcacccacga agtgatactg
     4141 cggcattact cgccgcgcat gtttaccgcg gtcgcggtcg ccagcgccag cggctacctc
     4201 gtcgacaacg tgatcttcga gcgcacgccg ctgatccagt tttcgatgtt cggcgatttc
     4261 cgcggcgtcg agctgccgct gttcgctttg ctgggcgtgg cctgcgggct gctcgcggta
     4321 ctgttcatga aaatgctcga ccgagttcgg caactggcgg cgcagacggc gctgccagcg
     4381 ccgctcaagc cgatgctggc cggcttcctg ctgtcgctgg tcgcctggca ggttcccgag
     4441 gtactcggcg ccggcgagga agtgtttcgc accgcggtcc acggctacct gttcagcagc
     4501 ggcgacctgc tggtgatcct ggtggccaag atactggtga ccgcgatgtg tattggcttc
     4561 ggcttcgccg gaggcgtatt cttcccgtcg ttgctgatcg gcgcactgtt cggcgcgctg
     4621 ttcgcccacc tcgtcccggc gcagctgctt gacggctact cgggcctgtc gacctacgcc
     4681 gtctgcggca ccgccgccct gatgggcccg gtgatcggcg caccgatgac cgcgctggtg
     4741 ctggtgttcg agttcacccg cagctacgag attaccgtcg ccgcgatgct ggcgatcgtc
     4801 ttcgccaatc tggtctctca tcactggcac gcgcgatcac agtgggattt cgaactcggc
     4861 gcgcggggta tcgacctgtc gctgggccgc gaacgcgctt atcttcggca ccagaaggtc
     4921 gttcggctgg ccatcgatgc cctgccggtg ctggcgtcgg cgcacaccgt cggcggacta
     4981 cgcgagcaac tgcgcacggc cactaccaaa accgcggtga tcgtcgacga tgacggtaac
     5041 tacctgggcc aggtatcgcg aaccgacctc ggcaaattgc agggtagtga gacagtggca
     5101 tcgctcgact tcgaaaacgg caagctgttc gacgaaacga ccagcatctg ggatgcgatg
     5161 caggcgatgc gcaattatat cggtgaagcc ataccggtgg tcgattcgcg gaccggacgc
     5221 tacctcggcg cggtccccga atcggcggtc atcaatgcat atctggacgc agcacaggag
     5281 ctgcgccgcg aagaacacga ggtctgaact atggaatttc atcaattgat cgtcaaccag
     5341 gcggccaacg cgctcgacga ggacgtcggc agcggcgata tcagcgccga gctgatcgat
     5401 gcctcggcac gcctggatac cgaattactg gtgcgcgagg atgccgtcct ctgtggttgc
     5461 ccgtggttcg acgaagtgtt tcgccagtgc gatgacacca tcgacatcga atggctggcg
     5521 cgagacgctg acgcgatcac cgccggcagc gtaatcgcgc gcatttcggg tgccgcccgc
     5581 ccgttactga cggccgagcg cagcgcgctg aatttcctgc aaacgctatc ggcgaccgcg
     5641 accgtgacgc ggcgctacgc cgacattctg cgcggtaccg gatgccgcat actcgatact
     5701 cgaaaaacca ttccacagct gcgccatgcg caaaaatacg cggtcgtctg cggcggcggg
     5761 accaatcatc gaatcggtct gttcgatgcc tacctggtca aggaaaacca cctcgcggcc
     5821 tgcggcggca tcgcgccggc cgcggcgcgg gcgcgcgagc tgcatcccga caggctgctc
     5881 gaaatcgagg tcgagaccct cgagcaattg
//
LOCUS       DATCZV010000483         2606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_40951, whole genome shotgun sequence.
ACCESSION   DATCZV010000483 DATCZV010000000
VERSION     DATCZV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40951"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..879
                     /locus_tag="VIW27_10895"
     CDS             154..879
                     /locus_tag="VIW27_10895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY5740224.1"
                     /translation="MKDLLVLQHIEIEDPGYLMDLMRADDMRITQIELDAGESIPADL
                     DAFDAMLCMGGPMDTWMELEYPWLVDEKRYIRDWVIERQKPFLGFCLGCQLLGEVLGG
                     RVERSTPPEIGLLDIEMSAAAAHDPLFADYPSVIKALQWHSYEVRGLDSIPGVTLLGS
                     SASTPYQIFSYREHAWAMQFHVEVRTDTVQQWGEVPVYRDALESSLGSGALQQLDDEA
                     RAHMPEMNRLAGLLYENFKLRLS"
     gene            complement(1002..1532)
                     /locus_tag="VIW27_10900"
     CDS             complement(1002..1532)
                     /locus_tag="VIW27_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740225.1"
                     /translation="MSRAEQYQDFMRWQCRLRMRAMRERDGRPSEGMSAGIYSINGGE
                     QQASISFLIVRRDSSDRSREFRHIVRKTTEPREWMKNGLRILAERHYQQAGDFEHELT
                     ALFSLDSQAAYALLKAGQCRLEFAEGSIAHGFDFDVRSLNQQDELFQATYWHNHLFNP
                     TLPGKVRVLGFAPRLS"
     gene            1943..>2606
                     /locus_tag="VIW27_10905"
     CDS             1943..>2606
                     /locus_tag="VIW27_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740226.1"
                     /translation="MTEFSILVIDKHPEYLALANQQITAALLGNKKPKLMQQISATGL
                     EIALLSGDYDVAFIDYETVAGLCNGYVARWLKNICNKPNCSQIVVVSATGDELLAVKM
                     ILAGAADYLPKRLLSSRLIEVATKRALSNSSTPSTSSEATSENVIHLQDPQRAQDFAD
                     LSSTEDATVIDLSQPIKPAINIDGYEITRELNKSKDTSLYLARSLERQELVVLKLVSL
                     SGS"
BASE COUNT          530 a          795 c          768 g          513 t
ORIGIN      
        1 gccgtttcac tggtacaagg agcatgtgtt gcgcggcgcc ctggagcatg attttccacc
       61 gcactatgtc gatgcgatac gcaccacgcc gtcgatcgac gaccacgacc tggaacgcgc
      121 ccggcgcgag ttgtcgattt acggagagcg gtgatgaaag acctgctggt gctgcagcac
      181 atcgaaatcg aggatccggg ctacctgatg gatttgatgc gtgccgatga catgcgcatc
      241 acgcagatcg aactcgatgc cggcgaatcg attccggccg acctcgacgc cttcgacgcg
      301 atgctgtgca tgggtggccc gatggatacc tggatggaac tggaatatcc ctggctggtc
      361 gacgaaaagc gctatattcg cgactgggtg atcgagcggc aaaagccgtt cctcggtttt
      421 tgcctcggct gccaactgct cggcgaagtg ctcggcgggc gcgtcgagcg ctccacgccg
      481 ccggaaatcg gcttactcga catcgagatg agcgcggcag cggcacacga cccgctgttt
      541 gccgactacc cgtccgtgat caaggcgttg caatggcatt cttacgaggt gcgcgggctc
      601 gattccatcc ccggtgtcac actcctcggc tcgtcggcga gcacgcctta ccagatcttc
      661 agctaccgtg aacatgcctg ggcaatgcag ttccacgtcg aggtgcgcac cgacacggtg
      721 cagcagtggg gcgaggtgcc tgtataccgc gacgcgctcg agtcgagcct cggcagcggt
      781 gcattgcaac agctggatga cgaggcgcgc gcgcatatgc cggagatgaa ccggctcgcc
      841 ggcctgctct acgagaattt caaactgcgc ttatcctgaa cattgctccg ctcggtctcg
      901 agcctctacc ggcaccaacg gattggcctg ctgttggatt tcaagctgac aagttttttg
      961 acgatcgtgc tacggcagcg gcccgaatag cggtgctata ttcaggatag ccgcggcgca
     1021 aagccgagca cgcgcacctt gccgggcagg gtcgggttga acaggtgatt gtgccagtag
     1081 gtcgcctgga acagctcgtc ctgctggttc agcgagcgca cgtcgaaatc gaaaccatga
     1141 gcgatactgc cctcggcaaa ttcgagccgg cactggccgg ccttgagcag cgcgtaggcg
     1201 gcctgcgagt cgagactgaa cagtgccgtc aactcgtgct cgaaatcgcc ggcctgctgg
     1261 tagtgacgtt cggcgagaat gcgcagcccg tttttcatcc actcgcgggg ttccgtcgtc
     1321 ttgcgcacga tgtgcctgaa ttcgcggctg cggtcggacg agtcccggcg cacgatcaga
     1381 aagctgatgc tcgcctgttg ctcgccgcca ttgatcgagt aaatgccggc gctcatgccc
     1441 tcggaggggc gcccgtcgcg ttcacgcatc gcgcgcatcc gcaatcggca ctgccagcgc
     1501 atgaaatcct ggtattgctc ggccctgctc atttcgtaag tccgggttgt attgacggtc
     1561 gccgacagta taacggattc cccgggcgac taccggcgcg gacccccgac actgcgccat
     1621 tcaacagcgg tacgccagtc gcggcctgta acggataagc agcgcgtgcc gcgcgatccg
     1681 ctgccggatt accgcggtac cgacccggct gccggcgctt gtcctcgtga ttcggcagtc
     1741 gacgtgtaag cggatcggcg caaatttgtt acccgacctc gtgctgttcg cgacaaaatc
     1801 agaactgatt cgcaactccg ctcatgcggg cttgcatttt tgagggcgta aactaaaacc
     1861 aaaacaccct tagcgttcaa gttgaccgcc tccgcgaccc gcttcaaagg tttacccggc
     1921 agtcgaaaaa agttaggaat ccatgactga gttcagcatc ctggtgatcg acaagcaccc
     1981 cgagtacctg gcgttggcga accaacagat cacggctgcg ctgctcggca acaaaaagcc
     2041 gaaactgatg cagcaaatct ccgcaaccgg gctcgagatc gcgctgttgt cgggcgatta
     2101 cgacgtcgcc ttcatcgatt acgaaaccgt cgccggcctg tgcaacggct acgtcgcgcg
     2161 ctggctgaaa aacatatgta ataaaccgaa ttgctcgcaa atcgtcgtcg tctcggcaac
     2221 cggcgacgaa ttgctggcgg tgaaaatgat tctcgccggc gccgcggatt acctacccaa
     2281 gcggctgctg agttcccgtc ttatcgaggt agctaccaag cgggcgttgt caaactcgag
     2341 cacgccctcg acctcgagtg aggctacctc agaaaatgtg attcacctgc aggaccccca
     2401 gcgagcgcag gatttcgctg acctgtcgtc gacggaagat gcgaccgtca tcgacctgtc
     2461 gcagccgatc aagccggcga tcaacatcga cggctacgaa atcacccgcg agctgaataa
     2521 atccaaagat acctcgctgt acctggcgcg aagcctcgaa cgccaggagc tggtagtgct
     2581 caaactggta tcgctgtccg gttcca
//
LOCUS       DATCZV010000484         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_92604, whole genome shotgun sequence.
ACCESSION   DATCZV010000484 DATCZV010000000
VERSION     DATCZV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92604"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VIW27_10910"
     CDS             complement(<1..1462)
                     /locus_tag="VIW27_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY5740227.1"
                     /translation="MVDFLNGTTLLFTSFMGVTIFIGGLLGGLVFGAIPGLNVLTLAA
                     VLLPFTAWMSAEHAIMLYGVIYVSGVYGGAITAILFNIPGSVENAPTAFDGYPMTRQG
                     KASRAIGYAVTCSAIGGTFSAILMMIATEPIADFAISTFGPVEITALIFFGLTVVAGV
                     GSRSLAKGWLSLGLGLLIGSVGGDPVGAVPRFTFGSLYLTGGLGFIPLILGLFAVSEV
                     FLQGQRIAQGKYEAPKISVDYPSFLEFWAMRYGVIRSTVIGWFSGILPGVGATLAAFL
                     SYNEAVRWSKTPEKFGTGIPEGVVAPETANNAATGAAMIPLLALGLPGGALTAMMIGV
                     FNVHDMEVGPLIMMEAHDLVWILFASMFWASLAILMLGIVESKCIVHMLRIPFTMLAP
                     GILLFSTIGAYSLRGNVFDIYTMFIAGIAGFFLRRSDYSVPAIVMGVILGQIGENSMA
                     QALTMLDYNFLGFFSEPIAGVLVSIGLLTVATSIFRR"
     gene            complement(1475..2029)
                     /locus_tag="VIW27_10915"
     CDS             complement(1475..2029)
                     /locus_tag="VIW27_10915"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY5740228.1"
                     /translation="MTSSDKEPTRLRDRLVWEALAWLGFTLVVYLFTRDFDAPLPSYQ
                     LGAAFWPQVVLVITAIAAITLLVSRFVRGVETDDADDGPAHLEDIPAPSGGIPIITLA
                     MFILPLIWVFALHQIGFLLSTPFFLIGFTWLIGIRRLRTLLGFSLGFYAVLVVVFYKL
                     IFTPLPMGAGWFHSISGEIIALIQ"
BASE COUNT          492 a          609 c          664 g          324 t
ORIGIN      
        1 cccggcggaa gatgctcgtg gccaccgtca gcagtccgat ggaaacaagg acgccggcga
       61 tcggttcgga aaagaagcca aggaaattgt agtccagcat tgtcagcgcc tgggccatcg
      121 aattctcgcc gatctggccg agaatgaccc ccatgacgat ggccggcacg gaataatcgc
      181 tgcggcgcag gaagaagccc gcgataccgg cgatgaacat cgtgtagatg tcaaagacgt
      241 tgccgcgcag cgaataggcg ccgatcgtcg agaacaggag gatgccgggc gccagcatcg
      301 tgaagggtat ccggagcata tggacgatgc atttggattc cacgatgccg agcatcagga
      361 tcgcaaggct cgcccagaac atcgacgcga acaggatcca gaccagatcg tgcgcctcca
      421 tcatgatcag cgggcccact tccatatcgt gcacgttgaa gacgccgatc atcatggcgg
      481 tcagggcgcc gccgggcagg ccaagcgcca gcagcggaat catggcggcg ccggtcgccg
      541 cgttgtttgc ggtctccggc gcgaccacgc cttcgggaat cccggtgccg aatttttccg
      601 gtgtcttgga ccagcggacc gcttcgttat aggaaaggaa ggcggccaat gtcgcgccga
      661 cgcccggcag gatgccgctg aaccagccga tcaccgttga acggatgacg ccgtagcgca
      721 ttgcccagaa ttccaagaag gagggatagt cgacggaaat tttcggcgct tcatatttgc
      781 cttgggcgat cctttggcct tgcaggaaca cttcgctgac cgcgaacagc cccaggatca
      841 agggaatgaa gccgagcccg ccggtaaggt acagcgaacc gaaggtaaat cgcggcaccg
      901 cgccgacggg gtcgccgccg accgaaccga tcagcaagcc caatccgagc gagagccagc
      961 ccttggcgag cgagcgcgac ccgacgccgg caacaaccgt gaggccgaaa aagatcaggg
     1021 cggtgatttc gaccggtccg aatgtcgaaa tcgcaaaatc ggcgatcggt tcggtcgcga
     1081 tcatcatcag aatcgccgag aatgtgccgc cgatcgccga acaggtgacc gcgtagccga
     1141 tcgcccggct ggcctttccc tggcgcgtca tcggatagcc gtcgaacgcg gtcggcgcgt
     1201 tttcgaccga gcccggaata ttgaacagga tcgcggtgat cgcgccgcca tagacgccgg
     1261 agacgtagat cacgccatag agcatgatgg catgttcggc cgacatccag gcggtaaagg
     1321 gcagcagcac tgccgcgagc gtcaggacat tgagcccggg gatcgcgccg aagaccagtc
     1381 cgccgagcag tcccccgatg aagatggtga cgcccataaa gctggtgaac agaagcgtcg
     1441 tgccgttgag gaaatcgacc atgatcgtcg cgccctattg aatcaaggcg atgatttcac
     1501 cgctgatcga gtgaaaccag ccggcgccca ttggcagggg cgtgaaaatg agtttgtaaa
     1561 agaccaccac gaggacggca tagaacccca gactgaagcc gagaagcgtg cgcagccgac
     1621 ggatgcctat cagccaggtg aagccgatca ggaagaacgg cgtcgacagc aggaaaccga
     1681 tttgatgcaa ggcgaaaacc cagatcagcg gcaagataaa catggcgagg gtgatgatcg
     1741 gaatgccgcc cgatggcgcc ggaatatcct ccaaatgggc ggggccgtcg tcggcgtcgt
     1801 cggtctcgac accccgcacg aaacgggaga caagaagcgt gattgcggca attgcggtga
     1861 tgaccagcac gacctgcggc cagaacgcgg caccgagttg atagctgggc aggggtgcat
     1921 cgaagtcgcg cgtgaagagg taaaccacca gcgtaaaacc gagccaggcg agcgcttccc
     1981 aaacgaggcg atcgcgcaac cgcgtcggct ccttgtcgct ggacgtcatg acattccccc
     2041 caatctaacg gtcttgtttt gaaacgtcgg cgtctcgccg gctgtaggg
//
LOCUS       DATCZV010000485         8389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_571235, whole genome shotgun sequence.
ACCESSION   DATCZV010000485 DATCZV010000000
VERSION     DATCZV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571235"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VIW27_10920"
     CDS             complement(<1..1250)
                     /locus_tag="VIW27_10920"
                     /inference="COORDINATES: protein motif:HMM:NF042699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6602 domain-containing protein"
                     /protein_id="HEY5740229.1"
                     /translation="MNNYQDWAQLVAKKLEHESMLLGAIGKDELDDAAATEAFVRAVL
                     QPFLPENYGIGSGRVVDAYGNYSDYLDIIVFNQNYPRIGMRGTHSAYLYESVLAAFAI
                     RAKFVRKSFADALDACASLAELETAVDKRALRRLAAKNGLKAGSNHTYVHQDPLRTAR
                     FELIGRPLSFVFGFSGFKFSHRQLQENIELWIENREKAHISTPMKCLPAVIATQGCFA
                     WRNAAPLALKGREMLGIGNDDAPVRLIVLQLLYLLNRRLQVAFDGYGLKPSLNRYLSH
                     FSVPKFEAGVGNVARVVKLAADKASSGPAKAASAKSVAGPKSPAQTVKSRSAEKPKPA
                     TESTPAVESKPVAESKPAAESKPVAESTPAAKSAPPAAAKPTGAAKPAAETAQATVPA
                     KSAASPESKPAPPAQKDSPFESSGV"
     gene            1434..3074
                     /locus_tag="VIW27_10925"
     CDS             1434..3074
                     /locus_tag="VIW27_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5740230.1"
                     /translation="MHLINPSASDFDGVCDAFRWSIPPRLNIAQQVCERHQGQGERVA
                     VYYENASGERCSYRFADLKQLSDRFANALRAVGIERGDRVAIVLPQTIETVVAHLAIH
                     KLGAVSLPLAILFGPEALEFRLRDSGARAAIVHRDRYDQVRALAPELPDLQRVIGCRC
                     GAGEDEFWQLLERASDHFEMQQTGPDDPACLIYTSGTTGPPKGALIAHRAVIGNFSGF
                     EMSQNFFPQAGDVFWTPADWAWTGGLWDALLPALNYGVPIVAYEGGGFDAERVCKLMA
                     DYGVSNAFIPPTALKMLRAVPGLDSRYALRLRAIMSAGEQVGEELIHWGRDALGLTIN
                     EMWGQTEFNYIVGNCSAIMAPKPGSMGKPYPGHRVDVIDADGEVLAAGEEGELAAWCD
                     DPVMFLGYWNNPEATREKITANWFRTGDVGYRDEDGFLWFVGRKDDVISSAGYRIGPG
                     EIEDSLLRHPAVLQAAVIGKPDELRGQIVKAFVVLTAGHEASPELAAEIQRSVRERLS
                     AHEYPREIEFIDELPMTTTGKVRRIELRARETAVTGSG"
     gene            complement(3156..3458)
                     /locus_tag="VIW27_10930"
     CDS             complement(3156..3458)
                     /locus_tag="VIW27_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740231.1"
                     /translation="MFKFFKHRDKAAKTEQKLAMQREMERRRAKGSQSDSMLRANIAS
                     KLKTYNAEEDTTISNFTLADDEIERKLAGKPARPANQDVWNQANPRETGRWKKENR"
     gene            3729..4652
                     /locus_tag="VIW27_10935"
     CDS             3729..4652
                     /locus_tag="VIW27_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5740232.1"
                     /translation="MTAVIETSDRPALGIALMLTGIAGFAVMDALIKWLTADYSIIQV
                     AALRSWFGLPLLCLVALRGGGIHLLKTRRPMVHGGRFLLVLGLSLTFFWALSQMKLAD
                     AIAITFAAPIFITALSVPLLREAVGCHRWAAVGVGFVGVLVMLRPGVGVFQWAALVVL
                     VSVVFYALLMISTRAFKSTESTASLMLYPQLGMSLTGIFAVPFFWITPSLGDLGLFAI
                     AGMFGSVGVMCITQAFRLGPAAVITPFEYSALLWATLLGYLLWGDLPDLVTLAGAAIV
                     VASGLYIIYRETIKTGRAPEQVTSMGPDDTV"
     gene            complement(4856..6007)
                     /locus_tag="VIW27_10940"
     CDS             complement(4856..6007)
                     /locus_tag="VIW27_10940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY5740233.1"
                     /translation="MTVICDIGDLQKLARRRVPRMFYQYADSGSWSESTYRANQADFK
                     SIKLRQRVAVDISERSVKSEIVGQPAAMPVALAPVGLTGMQYPDGEIRAARAAQKFGV
                     PFTLSTMSICSIEDVAAHTEAPFWFQLYVMKDRDFIGRLIDRARDAGCSALMLTLDLQ
                     ILGQRHKDLRNGLAAPPKMSLNTALQLALRPRWCLGMLSTRRHGFGNIVGHVKGVSDL
                     SSLGAWTSEQFDPKLSWADIDWIRERWSGKLILKGILDVEDARLAARTGADALIVSNH
                     GGRQLDGARSSISALPEIVDAVGGDIEVHFDGGIRCGQDVLKAICLGASATYLGRAYI
                     YGLGARGEAGVTQALEIIHRELDTTMALCGERLLQNLGRHNLLEPPAGG"
     gene            6124..6786
                     /locus_tag="VIW27_10945"
     CDS             6124..6786
                     /locus_tag="VIW27_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740234.1"
                     /translation="MLSIRALSKSYLYADLRQVVLDALDFDMEPGSSVALLGESGSGK
                     STLLHLIAGLDRPDGGEISFAGERVDDASESALAAMRRSRMSLVFQQFHLIPTLDVLD
                     NLRFQAALCQRLDAGYEAELVERLGLGGQLRKYPQQLSRGQQQRVAIARALLHRPPLV
                     LADEPTGNLDEASSLEVMALFSELVKQAGSGLLMVTHSRAMADFMDRRVELHNGRLAE
                     IE"
     gene            6783..>8389
                     /locus_tag="VIW27_10950"
     CDS             6783..>8389
                     /locus_tag="VIW27_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5740235.1"
                     /translation="MKAGFGWVLKALLSHYWRHPWQTLFLAIGLVAGVGLWSAVQIIN
                     QHAEDSYREARNLLGAQAHYWIQNRRGDGIETAQYVGLRRAGFRQLFPLVELQVSTAA
                     GVSISIIATDLLALPAGALGSGDSDFAAAWLDFTRPPYQAWYPRALAAELGLEAGDQL
                     QLRDGRRLPPARLQAREQQGRRVLMDIGAAMALDGDSRIDYLAVGSLPPNEYRRLAAM
                     LPAGLELVENQQHIDLRELTASLHTHLTAMSLLSFAVGLFIVFNAVRFSLWYRRATLL
                     DLKLMGCAPRQLLAAIGLETLLWSLLGAGLGFGIGVVVAHALLPGLGASLQSLYDATV
                     GTELGVSPATLLKAWSITLLGLVWALGWPLYRLLRQSSLEAGSSLAGFEDEARVRRRL
                     ALAALLLVPLAAVAYQGATTAAQGFAVLGMMLFAAAWLLPMLLAAGLRVVAALLPRRQ
                     LLARWMVSDGWSQLPAFRSAMMALLLALTANLGVGTLVDSFRDAFTDWLEIRQSADIY
                     LRAPRLDLEQLQPSPANAGWLADSHRR"
BASE COUNT         1347 a         2607 c         2737 g         1698 t
ORIGIN      
        1 accccggacg attcgaacgg cgaatctttt tgcgccggcg gggccggctt cgattcggga
       61 ctggccgcgg atttggccgg cacagtcgcc tgcgcggttt ctgcggcggg cttcgcggcg
      121 cctgtcggtt tggcagcggc gggtggcgcg gattttgcgg ccggtgtcga ttcggcgacc
      181 ggtttcgatt ctgcggctgg tttcgattcg gcgaccggtt tcgattcgac agccggtgtc
      241 gattcggtag ccggtttcgg cttctcagcc gacctggatt taaccgtctg cgcaggcgat
      301 ttcggcccgg ccacggattt tgcgctcgcc gcctttgccg ggccgctgct agccttgtcg
      361 gcggcaagct tcaccaccct ggcgacattg ccgacgccgg cttcgaattt cggtaccgaa
      421 aagtggctca ggtaacgatt gaggctcggt ttcagaccgt aaccgtcgaa agcgacctgc
      481 aagcggcgat tgagcaggta aagcagctgc agcacgatca gccggaccgg tgcatcgtcg
      541 ttgccgatgc cgagcatttc ccggcccttc agcgccagcg gcgcggcgtt gcgccaggca
      601 aagcatccct gggtggcgat cactgccggc aggcatttca tcggtgtcga gatgtgggct
      661 ttttcgcggt tttcgatcca cagctcgatg ttttcctgca attgccggtg actgaatttg
      721 aagccgctaa agccgaacac gaatgacagc ggccggccga tcagctcgaa gcgcgcggta
      781 cgcaatggat cctgatgcac gtaggtgtgg ttggatccgg ctttcaagcc gttctttgcc
      841 gccagccgtc gaagagcccg cttgtcgacg gccgtttcca gttcggccag cgatgcacag
      901 gcgtcgagag catcggcaaa cgacttgcgc acgaacttgg cgcgaatcgc aaacgccgcc
      961 agcaccgact cgtacaggta ggccgagtgc gtgccgcgca tgccgatgcg cggataattc
     1021 tggttgaaga cgatgatgtc gaggtaatcc gaataattgc cataggcatc gacgacccgg
     1081 ccgctgccga taccgtaatt ctcgggtaga aacggttgca gcaccgcgcg cacgaaggcc
     1141 tcggtggcgg ctgcgtcatc gagctcgtcc ttgccgattg cgccgagcag catggattcg
     1201 tgttccagct ttttggcgac cagttgtgcc cagtcttggt agttattcat gtggcgaatt
     1261 acccggacgc gctgcctcac gtctaaatct gtagtaaggt taaaaaattc tacactaata
     1321 tcagtgcttt attaagtaat gtaaaaccta aggtatagac cctgaggcga atttcacaac
     1381 ttcaaatatt tcatgttgcg gtatgatgcg agaatttgca gatgggttgt gacatgcatc
     1441 taataaaccc gtccgccagc gatttcgatg gcgtctgcga tgcctttcgc tggtcgattc
     1501 cgccacggct caatatcgcg cagcaggtct gcgaacgcca ccaggggcag ggcgagcgtg
     1561 tcgccgtcta ctacgaaaac gcgtccggcg agcgctgcag ttaccgtttc gccgacctga
     1621 aacagttatc cgaccgtttc gccaacgcgt tgcgcgcggt cggtatcgag cgcggcgacc
     1681 gggtggctat cgtgctgccg caaaccatcg agaccgttgt cgctcacctc gcgatccaca
     1741 agctcggcgc ggtgtcgctg ccgctggcga tcctgttcgg tcccgaagcg ctggaatttc
     1801 gcctgcgcga cagcggcgcc cgggcggcca tcgtgcaccg cgaccgctac gaccaggtac
     1861 gcgcgctggc gccggaattg cccgacctgc aacgggtgat cggctgccgt tgcggtgccg
     1921 gtgaggacga gttctggcaa ttgctggaac gcgcctccga ccacttcgaa atgcagcaaa
     1981 ccggccccga cgacccggcc tgcctgatct atacctcggg taccaccggt ccgccgaaag
     2041 gcgcgttgat cgcacaccgc gcggtgatcg gcaatttcag cggcttcgaa atgtcgcaga
     2101 atttctttcc gcaggcgggc gacgtgttct ggacgcccgc cgactgggcc tggaccggcg
     2161 gcctgtggga tgcgctgctg ccggcgctca actacggcgt gccgatcgtc gcctatgaag
     2221 gtggtggctt cgatgccgag cgggtttgca agctgatggc cgactatggg gtcagcaacg
     2281 cattcattcc gccgactgcg ctaaagatgc tgcgcgcggt accgggtctc gactcgcgct
     2341 atgcgctcag gttgcgcgcc atcatgagtg ccggcgagca ggtcggcgag gagttgattc
     2401 actgggggcg cgatgcgctc ggcctgacga tcaacgagat gtgggggcag accgagttca
     2461 attacatcgt cggcaattgc tcggcgatca tggcgccgaa gccgggctcg atgggcaagc
     2521 cgtatcccgg tcaccgcgtc gacgtcatcg acgccgacgg cgaggtgctg gccgccggcg
     2581 aggaaggcga gctcgccgcc tggtgcgacg acccggtgat gtttctcggt tactggaata
     2641 accccgaggc gacgcgcgaa aaaatcaccg ccaactggtt tcgcaccggc gacgtcggtt
     2701 accgcgacga ggacggcttc ctgtggttcg tcggccgcaa ggacgacgtg atttccagcg
     2761 ccggttaccg tatcgggccc ggcgagatcg aggattcgtt gctgcgccac ccggccgtgc
     2821 tgcaggccgc ggtaatcggc aagcccgacg agctgcgcgg ccagatcgtc aaggctttcg
     2881 tggtgttgac tgccggtcac gaggccagcc cggaactcgc cgccgaaatc cagcgctcgg
     2941 tgcgcgagcg gctgtcggcg cacgagtacc cgcgcgaaat cgagttcatc gacgaactac
     3001 cgatgaccac caccggcaag gttcgccgca tcgaactgcg tgcgcgcgaa actgctgtca
     3061 cggggtcggg ctagataccc ctgcagagca ataatttttg ctcgaatcga taactcgcgg
     3121 tatggcggtt gtttgccgtc aaccacgctg ctccgttatc ggttttcttt tttccatctg
     3181 cccgtttcgc gcggattggc ctggttccag acatcctggt tcgccggcct ggccggcttg
     3241 ccggcaagct ttctctcgat ttcgtcgtcg gccagcgtga aattggagat ggtagtgtcc
     3301 tcctcggcat tgtaggtttt gagtttactg gcaatatttg cccgcagcat cgaatcgctt
     3361 tgcgacccct tcgcccggcg gcgctccatt tcgcgctgca tcgccagttt ttgctctgtt
     3421 ttcgcggcct tgtcgcggtg cttgaagaat ttgaacatat cgcgttgaac ctcgtctctg
     3481 aatccagtcc ggctatcctt tgagttgccg aaattcgcga ttgcgaagtc aatcgaagtt
     3541 acgcccaaaa ttgcagtcgg cgaaggaaat atttcacctt tggcgagtga attaagtgta
     3601 aaagcacggg ccgtggatcg agggccgatt tcggctccgg cgcgccgccg aaagcctaaa
     3661 gttgtttgcc tgcaccggat ttcgtgtatc gtgtcggcag ctccctgttg cggataaatc
     3721 cagccactat gacagcagtc atcgagactt ccgatcggcc agctctcggc attgcgctga
     3781 tgctgaccgg catcgccggc ttcgcggtga tggacgcgct catcaagtgg ctgaccgccg
     3841 attactcgat catccaggtg gccgcgctgc gcagctggtt cggcctgccg ctattgtgcc
     3901 tggtcgcctt gcgcggcggt ggcatccact tgctgaaaac ccgacgtccg atggtgcacg
     3961 gcggccgctt cctgctggta ctcggcctgt cgctgacctt tttctgggcg ctgtcgcaaa
     4021 tgaagctcgc cgacgcgatc gcgatcacct tcgcggcgcc gatcttcatc accgcgctgt
     4081 cggtgccact gctccgcgaa gcggtcggct gccatcgctg ggcagcggtc ggcgtgggct
     4141 tcgtcggcgt gctggtgatg ctgcgcccgg gggtcggcgt gttccagtgg gccgcgctgg
     4201 tggtgctcgt cagcgtcgtc ttctacgcgc tgctgatgat ttccacgcgc gccttcaagt
     4261 cgaccgaaag caccgcctcg ctgatgctct atccgcagct cggcatgtcg ctgaccggca
     4321 ttttcgccgt gccctttttc tggatcacgc cgagtctcgg cgatctgggt ctgttcgcga
     4381 tcgccgggat gttcggcagc gtcggcgtga tgtgcataac ccaggccttc cggctcggcc
     4441 cggccgcggt gatcacgccg ttcgaatatt ccgcactgct gtgggcgaca ttgctcggct
     4501 acctgttgtg gggtgatttg ccggacctgg tgacactggc cggtgcggcg atcgtggtcg
     4561 ccagcggcct ttacattatt taccgcgaga cgatcaaaac cggccgcgcg ccagagcagg
     4621 tcaccagcat gggtcccgac gataccgtct gacgaatagc gtcggcgcga tagtgcagga
     4681 cagggatgcc gagtgttgtg ccccggcctg ttgcggccgg cgttgatcgg gaacgctatc
     4741 gtccgctccg ctttgcctaa attggatggt atgaataggg tgccggtatt gcgactggag
     4801 tcgaaaccgg tgggtgcggc gttggccgtt ttactggtcg ccgtcggccg gcgtctcagc
     4861 cgccggcggg cggctcgagc aggttatggc ggccgagatt ctgcagcagc cgttcgccgc
     4921 atagcgccat ggtggtgtcg agctcgcggt gaataatttc gagcgcctgg gtgacgccgg
     4981 cttcgccgcg cgcgccgaga ccataaatgt aggcgcggcc gaggtaagtc gcgctggcgc
     5041 ctagacagat cgccttcagc acgtcctgcc cgcatcgaat gccgccgtcg aaatgcacct
     5101 cgatgtcgcc gccgaccgcg tcgacgattt ccggcagcgc gctgatcgac gagcgcgcgc
     5161 cgtcgagctg gcggccgccg tgattggaga cgatgagcgc gtcggcgccg gtcctcgccg
     5221 ccaggcgggc atcctcgaca tcgagtatgc ccttgaggat caacttgccg ctccagcgct
     5281 cgcgaatcca gtcgatgtcg gcccacgaca gcttcggatc gaactgttcg ctggtccagg
     5341 cgcccagcga cgacaggtcg ctgaccccct tgacgtggcc gacgatgttg ccgaagccgt
     5401 ggcgccgcgt ggacagcatg cccaggcacc agcgcgggcg cagggcgagt tgcagagccg
     5461 tgttcagtga cattttcggc ggcgccgcga gtccgttgcg caggtctttg tgccgctggc
     5521 cgaggatttg caggtcgagc gtcagcatca gcgcgctgca gccggcgtcc ctggcgcggt
     5581 cgatcagccg accgatgaaa tcgcggtcct tcatcacgta gagctggaac cagaaaggtg
     5641 cctcggtgtg cgcggcgaca tcctcgatcg aacagatgct catcgtcgac agcgtgaacg
     5701 gcacgccgaa tttttgcgcc gcgcgtgcgg ccctgatctc gccgtccggg tattgcatgc
     5761 cggtcaggcc gaccggcgcc agcgctaccg gcatggcggc cggctggccg actatctcgg
     5821 acttcaccga gcgctcgcta atgtcgacgg caacccgctg gcgcagcttg atggacttga
     5881 aatcggcctg gttggcgcgg taggtcgact cactccagga acccgaatcg gcgtactggt
     5941 agaacattcg cggcacccgg cggcgcgcca gtttttgcag gtcaccgata tcgcaaatca
     6001 cggtcatgcg taatccctcg aaagccgtgc cagtgtactg caaagcgcgt gcttttccat
     6061 ggccggagca gaaacgcgcg ataaattaca ggctgtcgga aaattcggtt agcattggac
     6121 gcgatgctct cgatacgcgc cctcagcaag tcctacctgt atgccgacct gcggcaagtg
     6181 gtgctcgacg cgctcgattt cgacatggag ccgggttcgt cggtcgccct gctgggcgaa
     6241 agcggtagcg gcaagtcgac gctgttgcac ctgatcgccg gactcgatcg ccccgacggc
     6301 ggtgagatca gtttcgccgg agagcgtgtc gacgacgcct ccgagtcggc gctggcggcg
     6361 atgcggcgct cgcgcatgag cctggtgttt cagcaatttc acctgatccc gacgctcgac
     6421 gtactcgata acctgcgttt ccaggctgcg ctatgccagc gactcgatgc cggctacgag
     6481 gccgagctgg tcgagcgcct cgggctcggc ggtcaactcc gcaagtaccc gcagcaattg
     6541 tcgcgcggcc agcagcagcg cgtggcgatt gcccgtgcgc tgctgcacag gccgccgctg
     6601 gtgctcgccg acgaacccac cggcaacctc gacgaggcgt cgagcctcga ggtcatggcg
     6661 ctgttcagcg agctggtgaa acaggccggc agcggtctgt tgatggtcac ccatagccgc
     6721 gcgatggcgg atttcatgga ccggcgagtc gaactgcaca acggccggct cgcggaaatc
     6781 gaatgaaggc cgggtttggc tgggtgctga aggcgctgct gagtcattac tggcggcatc
     6841 cttggcaaac cctgttcctg gccatcggcc tggtcgccgg tgtcggcctg tggtcggcgg
     6901 tgcagatcat caaccagcac gccgaggaca gttaccgcga ggcgcgcaac ctgctcggcg
     6961 cgcaggccca ctactggatt cagaaccgcc gcggcgacgg tatcgagacc gcgcagtacg
     7021 ttggcttgag gcgcgccggc tttcgccagc tgtttccgct ggtcgagctc caggtcagca
     7081 ccgcggcggg cgtgtcgatc agtatcatcg ccaccgattt gctggcgctg cccgctggcg
     7141 cgctcggcag cggcgacagc gatttcgccg ccgcctggct ggatttcacg cggccgccct
     7201 accaggcctg gtatccgcgg gcgctggccg ccgaactcgg gctcgaggcc ggcgaccagc
     7261 tgcagttgcg cgacggcagg cgcctgccgc cggcgcggct gcaggcgcgc gagcaacagg
     7321 gcaggcgggt gttgatggat atcggcgcgg cgatggcgct cgacggcgac tcgcgcatcg
     7381 attaccttgc ggtcggcagc ctgccgccga acgagtatcg gcgactggcg gcgatgctgc
     7441 ccgccggcct cgagctggtc gagaaccagc agcatatcga tctgcgcgag ctgaccgcat
     7501 cgctgcacac ccacctgacc gcaatgagtc tgctatcgtt cgcggttggc ctgttcatcg
     7561 tcttcaacgc ggtgcgtttc tcgctgtggt atcgccgcgc gacgctgctc gacctcaagc
     7621 tgatgggctg cgcgccgcgg caactgctgg cggcgatcgg gctcgaaacc ctgttgtgga
     7681 gcctgctcgg cgccgggctc ggtttcggga tcggcgtggt cgtcgcgcat gcgttgctgc
     7741 caggcctcgg cgccagcctg caaagcctct acgatgcgac cgtcggcacc gagctcggtg
     7801 tcagtccggc gaccctgctg aaagcctgga gtattacgct gctggggctt gtctgggcgc
     7861 tcggctggcc gctttatcgg ctgctgcggc aatccagcct cgaagccggc agcagcctgg
     7921 ccggcttcga ggacgaggcg cgtgtgcggc ggcggctggc gctggccgcg ttgctgctgg
     7981 tgccgttggc ggctgtcgcc taccagggcg cgaccaccgc ggcgcagggc ttcgcggtcc
     8041 tcggcatgat gctgttcgcg gccgcctggc tgttgccgat gctactcgcc gccggcctgc
     8101 gggtggtggc cgcactgttg ccgcgccggc aactgctggc gcgctggatg gtcagcgacg
     8161 gctggtcgca attgccggcc tttcgcagcg cgatgatggc gttgttgctg gcgctgaccg
     8221 cgaacctcgg cgtcggcacc ctggtcgaca gttttcgcga tgcttttacc gactggctcg
     8281 agatcaggca aagcgccgat atctacctgc gcgcgccgcg cctcgattta gaacagttgc
     8341 agccgtcgcc ggccaatgcc ggctggcttg ccgatagcca tcgccgaat
//
LOCUS       DATCZV010000486         4489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_304394, whole genome shotgun sequence.
ACCESSION   DATCZV010000486 DATCZV010000000
VERSION     DATCZV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304394"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VIW27_10955"
     CDS             <1..723
                     /locus_tag="VIW27_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEY5740236.1"
                     /translation="DWVAKMPAATALDQWRQCLIGEACDADYVFDLHCDDDSLLHIFS
                     VPQLADNMQQLADWTGAAATLMAEDSGGNSFDEVWPAVWLRLAADCPDKPLPLAVVSC
                     TLEYRGQIDTFDQLNRSDALKLYGYFQEQGLIGGAPLDGKGRAPAASDLRATEYLTSP
                     GSGLLVYRVELGERVEKGQPVADLLRLDGDGAFVDRLPLPAGTSGVVISRQALKYVWP
                     GHNICKIVGEEILERGDYLLSD"
     gene            996..2078
                     /locus_tag="VIW27_10960"
     CDS             996..2078
                     /locus_tag="VIW27_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5740237.1"
                     /translation="MSAEKSPGDEPVAAVPNQHAKSGARATLKSVASALGVSTTTVSN
                     AFSRPDQLSSALREKILRESRVLGYYGPDPIGRALRSRELREVGVVFHHDLSYALSDQ
                     QSVEFLRGVASELDKHQLMLQVIPKLGRRMSLQAAFRTTADMMIVHTDISPEFVPELQ
                     SANKPFVLVDVSLPGLPCVGIRDRDGALLAMAHALSKKPQKVILLGFQMDPAEREFLS
                     RNPNPPFRHSVVADRAAGYLAAARAAGFSPHDLIWVEIDDRFPESTASHLARIRQFDL
                     RRGERVAIVGMSDRIVLDAMHSVRNWSDIEIASMVGFDDIPAAAEAGLTTIRQDVYAK
                     GRLAARVLLDGEPAVRLPVELVVRDT"
     gene            2090..4330
                     /gene="glgB"
                     /locus_tag="VIW27_10965"
     CDS             2090..4330
                     /gene="glgB"
                     /locus_tag="VIW27_10965"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY5740238.1"
                     /translation="MTKTKTEKQADPGAAKDSPTLERGEMDAICAGRHASVFAVLGLH
                     ANSSGKGLVLRAFVPGAGKLEVLARNGRKLGELSRVHDGGLFEKIFSNRRKRFDYQLR
                     IDGETCVEDPCRFPGLLQSNDIYLFAEGSSEHAYRWMGAHARRVDGVDGVHFVVWAPS
                     ARRVSVVGDFNGWDGRRHVMRVDHGSGIWEIFIPAVGEYAHYKFEIVAANGRLLPLKS
                     DPYARSMQHAPETASRVVLDDSYDWQDDDWMATRAKLDHRQQPISIYEVHAGSWRRKH
                     EYDNRYLSYLELAEELIPYALEMGYTHLELMPISEYPFDGSWGYQPIGLFAPTIRFGT
                     PREFRRFVERCHLAGLGVLLDWVPGHFPTDEHGLGRFDGSCLYEHADPRRGFHPDWNT
                     LIYNYGRNEVRSFLLSNANYWLEEFHIDGLRVDAVASMLYLDYSRQHGEWLPNKHGGR
                     ENLEVISLLRDVNRRVHARHPGALMIAEESTAWPGVSEPVYNGGLGFGFKWNLGWMND
                     SLKHMARDPIHRRYHSNELTFGIHYAWSEKFILPLSHDEVVHGKGSLLGRMPGDEWRQ
                     FANLRAYLGFMWAQPGKKLLFMGGEFAQRREWNHDRSLDWHLLKHPLHAGVQRLVRDL
                     NRLYAGVPALHRLDASPEGFEWLQADSSEQSVFAWIRRGGDGEREIVAIANLTPQTHH
                     GYRVGAPRAGRYHERLNTDAADYGGSGQGNLGGVLAEDRGYDGQPASMVVTLPPLATV
                     FFEFAE"
     gene            4346..>4489
                     /locus_tag="VIW27_10970"
     CDS             4346..>4489
                     /locus_tag="VIW27_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740239.1"
                     /translation="MQVETGNHYPLGAVFDGEGTNFALFSEHAERVELCLYDADGRQQ
                     IARI"
BASE COUNT          803 a         1425 c         1463 g          798 t
ORIGIN      
        1 gactgggtcg cgaagatgcc cgccgccacg gcgctcgacc agtggcggca atgcctgatc
       61 ggcgaagcct gcgacgccga ctacgtgttc gacctgcact gcgacgacga ttcgctgttg
      121 catatctttt cggtaccgca gctcgccgac aatatgcagc aactcgccga ctggaccggc
      181 gccgcggcga cgctgatggc ggaggacagc ggcggcaact cgttcgacga agtctggccc
      241 gccgtctggc tgcggctggc cgccgactgc ccggacaagc cgctgccgct ggcagtcgta
      301 tcctgcacgc tcgagtatcg cggccagatc gataccttcg accaactgaa ccggtcggat
      361 gcgctaaagc tgtacggcta cttccaggaa cagggactga tcggcggcgc gccgctggac
      421 ggcaagggca gggcgccggc cgccagcgac ctgcgcgcca ccgagtacct gacttcgccg
      481 ggcagcgggc tgctcgtcta ccgggtcgag ctcggcgagc gcgtcgaaaa agggcagccg
      541 gtagccgacc tgctgcggct cgacggcgac ggcgccttcg tcgatcggct gccgctgccg
      601 gcgggtactt cgggcgtcgt catctcgcgc caggcattaa aatacgtctg gcccggccac
      661 aacatctgca agatcgtcgg cgaggaaatt ctcgagcgtg gcgactacct gttgagcgat
      721 tgaggccgtc gccttcggct ccactcgaag gtttcgcgat tacgaccctg gaaaacccgt
      781 tgatccggga tggtcgcgag cgcgcggcgg cagtccggcg atgatgtctt aatcgttcaa
      841 gttaggcttg cagaaatatc ttgaacgatt aagatcattg gtggtgtaat agccgcgtcg
      901 gtatcggcgg gtacccggat tcatcgacag cggggcgatc agtctgccca cggatagact
      961 gtcagttcag taaccaggca accaggaaaa ccagtttgag cgcggaaaaa tctccgggtg
     1021 acgagcctgt cgccgcggtc ccgaaccagc atgccaaaag cggtgcgcgc gctaccctga
     1081 agtcggtggc cagtgcgctc ggggtcagca cgaccacggt atccaatgcg ttcagccgcc
     1141 ccgatcaact gtcgagtgcg ctgcgcgaga aaatcctgcg cgaatcgcgc gtgctcggct
     1201 attacggccc cgatccgatc ggcagggcgc tgcgcagccg ggaactgcgc gaggttggcg
     1261 tcgtgtttca ccacgacctc agctatgcgc tgagcgatca acaaagtgtc gagtttctgc
     1321 gcggcgtcgc cagcgaactc gacaagcatc agctgatgct gcaggtgatt ccgaagctgg
     1381 gccgccgcat gtcgctgcag gccgcgtttc ggaccaccgc cgacatgatg atcgtgcaca
     1441 ccgatatcag ccccgaattt gtccccgaac tgcagtccgc caacaagccc ttcgtgctcg
     1501 tcgacgtcag cctgccgggc ttgccctgcg tcggcattcg tgatcgcgac ggcgcgctgc
     1561 tggcgatggc ccacgcgctg tcgaaaaagc cgcaaaaagt catcctgctg gggttccaga
     1621 tggaccccgc ggaaagggaa ttcctgagcc ggaatccgaa tccgccgttt cgccacagcg
     1681 tggtcgccga tcgcgccgcg ggttacctgg ccgcggcgcg cgccgccggc ttttcgccgc
     1741 acgatttgat ctgggtcgaa atcgacgatc gctttccgga atcgaccgcc agccacctgg
     1801 cgcgtattcg gcaattcgac ctgcggcgcg gcgagcgggt ggcgatcgtc gggatgagcg
     1861 atcgtatcgt gctcgacgcg atgcactcgg tgcgcaactg gagcgatatc gagattgcct
     1921 cgatggtcgg tttcgacgat attccggcgg ccgccgaggc cggcctgacg acgattcgcc
     1981 aggacgtgta cgccaaagga cggctcgccg cgcgggtgtt gctcgacggc gagcccgcgg
     2041 tacgcctgcc ggtggaactc gtggtgcgcg acacctgagc ggtacgcaga tgacgaaaac
     2101 gaaaaccgaa aagcaggctg acccgggtgc ggcaaaggac tcgccgacgc tcgagcgcgg
     2161 cgaaatggat gccatctgcg cgggacgtca cgcgagtgta ttcgccgtcc tcggcctgca
     2221 tgccaactcg tcggggaagg gtctcgtact gcgcgccttc gtcccgggcg ccggtaagct
     2281 cgaagtgctg gcccgtaatg gccgaaaact tggcgagctt tcccgcgtgc acgacggcgg
     2341 cctgttcgaa aagattttca gcaaccggcg caagcgtttc gattaccagc tgcgcatcga
     2401 cggcgagacc tgcgtcgagg acccctgccg ttttcctggc ctgctgcaaa gcaacgatat
     2461 ctacctgttt gccgagggca gcagcgagca tgcctatcgc tggatgggcg cgcatgcgcg
     2521 ccgcgtggat ggcgtcgacg gcgtgcattt cgtcgtctgg gcgccgtccg cgaggcgggt
     2581 atcggtagtc ggcgatttca atggctggga cgggcgtcgc cacgtgatgc gcgtcgacca
     2641 cggctcgggc atctgggaaa tattcatacc cgcggtgggc gagtatgcgc attacaagtt
     2701 cgaaatcgtc gccgccaacg gccgcctgtt gccgctgaaa tccgatccct acgcgcgttc
     2761 gatgcagcat gcgcccgaga ccgcgtcgcg cgtggtgctc gatgactcct acgactggca
     2821 ggacgacgac tggatggcga cgcgggcgaa gctcgaccat cgccagcagc cgatcagcat
     2881 ctacgaggtg catgccggct cgtggcggcg caagcatgag tacgacaatc gctacctgag
     2941 ctacctcgaa cttgccgaag aattgattcc ctacgcgctg gagatgggtt acacgcatct
     3001 cgaactgatg ccgatcagcg aatacccgtt cgacggctcc tggggttacc agcctatcgg
     3061 gctgttcgcg ccgaccattc gcttcggcac gccgcgggaa ttccgccgtt tcgtcgagcg
     3121 ctgccacctc gccggactcg gcgtgctgct cgactgggtg cccgggcatt ttccgaccga
     3181 tgagcacggc ctcgggcgct tcgacggcag ctgtctctac gagcacgccg acccgcggcg
     3241 cggctttcat cccgactgga acacgctgat ctacaattac ggccgcaacg aggtgaggag
     3301 tttcctgctc agcaatgcca attactggct ggaggaattc cacatcgacg gactgcgcgt
     3361 cgatgcggtg gcctcgatgc tctacctcga ttacagccgc cagcacggcg aatggctgcc
     3421 gaacaagcat ggcggccgcg aaaacctgga ggtcatctcg ttgctgcgcg acgtcaatcg
     3481 ccgcgtgcat gcgcggcatc ccggcgcgct gatgatcgcc gaggaatcga ccgcctggcc
     3541 gggggtctcc gagccggtct acaacggcgg cctgggcttc ggcttcaaat ggaacctcgg
     3601 ctggatgaac gacagcctca agcacatggc ccgtgacccg atccaccggc gctaccatag
     3661 caacgaattg acctttggca tccattacgc atggagcgaa aaattcattc tgccgctgtc
     3721 ccacgacgag gtcgtgcatg gcaagggatc gctgctcggc cgcatgcccg gcgacgagtg
     3781 gcggcagttc gccaacctgc gtgcctacct cgggttcatg tgggcgcagc ccggcaagaa
     3841 gctgttgttc atgggcggcg aattcgcgca gcgccgcgaa tggaaccacg accggtcgct
     3901 ggactggcac ctgctcaagc acccgctgca tgccggcgtg cagcgcctgg tgcgcgacct
     3961 caaccgcttg tacgccgggg tcccggcctt gcaccggctg gacgcgtcgc ccgagggctt
     4021 cgagtggctg caggcggaca gcagcgagca atcggtattc gcgtggattc gcaggggcgg
     4081 cgacggcgag cgcgaaatcg tcgcgatcgc caacctgacg ccgcagactc accacggtta
     4141 tcgcgtcggc gcgccgcgcg cgggtcgtta ccacgagcgc ctgaataccg atgccgccga
     4201 ctacggcggc agcgggcagg gcaatctcgg cggagtactt gccgaggatc gcggctacga
     4261 cggccagccg gcgtcgatgg tcgtcacact gccgccgctg gcgacggtgt tcttcgagtt
     4321 tgccgaatag caggttgccg gggctatgca ggtagagact ggtaaccatt acccgctggg
     4381 cgcggtgttc gatggcgagg gcaccaattt cgcattgttc tccgagcatg ccgagcgcgt
     4441 cgagctgtgt ctctacgatg ccgacggccg gcagcagatt gcgcgcatc
//
LOCUS       DATCZV010000487         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_463816, whole genome shotgun sequence.
ACCESSION   DATCZV010000487 DATCZV010000000
VERSION     DATCZV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463816"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIW27_10975"
     CDS             complement(<1..252)
                     /locus_tag="VIW27_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740240.1"
                     /translation="MSFAPLIAALLTVMVVAALIDLEAGLLAGLIIALLASRRVYESR
                     FRRLEKQLAELQEQLSAPRTPPADTAATAAAANAAVTGGQ"
     gene            625..897
                     /locus_tag="VIW27_10980"
     CDS             625..897
                     /locus_tag="VIW27_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740241.1"
                     /translation="MPRSFSEWIDFLYGPIFRVIGFFYRIYSANRARFDRLRENARVA
                     GLLRALAVIVLIGWILVWYLAPDERRHRLTEEIKQTLGMELPTAGN"
     gene            911..1090
                     /locus_tag="VIW27_10985"
     CDS             911..1090
                     /locus_tag="VIW27_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740242.1"
                     /translation="MTGVLLLAAIIGLFWLKDRLRNPLLARVAYSEPVARLAVVGAAL
                     SIIGLLLMASELFSA"
     gene            complement(1087..>2119)
                     /locus_tag="VIW27_10990"
     CDS             complement(1087..>2119)
                     /locus_tag="VIW27_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014108100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5740243.1"
                     /translation="QRNIQEFQFFWEGEKFQIGVSIGLVAIDETTASVTDLLKQADAA
                     CYVAKDMGRNQIQVYNPDDTILARRHGEMQWVARINQALEKKRFTLYAQAIEPLGSSS
                     GLHYELLLRMIGDHGEIIAPVNFLPAAERYDLMYKLDVWVVEEALTLLASNPEFMDEV
                     NFVSINLSGQSLTHFRFLNSVVGRIQSLDIDPQKICFEITETAAISNLRAATEFISRL
                     KELGCHFALDDFGSGLSSFGYLKNLPVDYLKIDGVFVRDVAIDPIDHAMVKSINDIGH
                     VMGMKTIAEFVENDAIRSMLKELSVDYAQGYEVGMPLPFADILGIQRHPESAVRTGDD
                     QPPKPVYVN"
BASE COUNT          397 a          605 c          664 g          453 t
ORIGIN      
        1 ttgtccgccg gtgacagcgg cattcgcggc ggcggccgta gccgcggtat ccgccggcgg
       61 cgttcgcggc gcggatagct gctcctgcaa ctcggcgagc tgtttctcca ggcgccgaaa
      121 acgcgactcg tagacgcgtc gactggcaag tagcgcaatg atcaggccgg ccagcaagcc
      181 agcctcgaga tcgatcaacg cggcgaccac catgaccgtc agcagtgcgg cgatgagggg
      241 tgcgaatgac atcagggatt aatatagcag tcgagcgcgg cggcgcggcg aaatagacgg
      301 ctggcggtcg aagggcgacg agttcggcat cggattagtc gaggattgga tcatcatcgt
      361 cagcattgcg atggcgaaag cataggccga ttcgggcgtt aagcaatgcg aaatatcacg
      421 ggtaccggct ttggttaccc aaggggtctc aattcgatac tttccatggt cgaggcccgt
      481 ggcgacttcg ccgtcccggc cggttggcat cggctgcctg ccggaatcca cgggtcgcgc
      541 cctggcgctg tcaaaagcac cgggcggcgg ttagaatgcg ggcggttcga tcgcgctgcg
      601 attttcagga ttccggtaac gagtgtgccc agatcttttt cagagtggat agattttctt
      661 tacggcccga tattcagggt tatcggcttc ttttaccgca tctacagcgc caaccgggcg
      721 cgcttcgatc gcctgcgcga aaacgcccgc gtcgccggcc tgttgagggc gctggcggtg
      781 atcgtgctga tcggctggat tctagtctgg tacctggcgc ccgacgaacg ccgtcatcgg
      841 ctgaccgagg agatcaagca aactctcggc atggaactgc cgactgcggg caactagcgt
      901 gggatttttc gtgaccgggg tcctgttgct ggcggcgatc atcggcctgt tctggctcaa
      961 ggaccgttta cgcaatccgc tgctggcgcg cgtcgcctat tcggagccgg tcgcacgcct
     1021 ggccgtggtc ggcgcggcgt tgtcgattat cggcctgctg ctgatggcct cggaactgtt
     1081 ctccgcctag tttacgtaga ccggtttcgg tggctggtcg tcgccggtgc gaaccgcgga
     1141 ctccgggtgg cgctggattc cgagaatatc cgcaaacggc agcggcatgc cgacctcgta
     1201 tccctgcgca tagtcgaccg agagctcctt caacatgctg cgaatggcgt cgttctcgac
     1261 gaactcggcg atcgtcttca tgcccatcac gtggccgatg tcgttgatcg atttgaccat
     1321 cgcgtgatcg atagggtcga tggccacatc cctgacgaat acgccgtcga ttttcaggta
     1381 atcgaccggc aggttcttca ggtagccgaa cgacgacagg ccgctgccga aatcgtcgag
     1441 cgcaaaatga caaccgagtt ctttcaggcg gctgatgaat tcggtggccg cgcgcaggtt
     1501 cgaaatcgct gcggtctcgg taatctcgaa gcagattttc tgcgggtcga tatcgagtga
     1561 ctggatcctg ccgaccaccg aattgagaaa tcggaagtga gtcagtgact ggcccgacag
     1621 gttgatcgaa acgaaattga cctcatccat gaactccggg ttcgaagcca gcagcgtcag
     1681 ggcttcctcg accacccaga catcgagctt gtacatcagg tcatagcgtt cggcagcggg
     1741 cagaaagttg accggggcga tgatttcacc gtggtcgccg atcatgcgca gcagcaattc
     1801 gtaatgcagg ccggagctcg agccgagcgg ttcgattgcc tgggcgtaaa gggtaaagcg
     1861 ttttttctcc agtgcctggt tgatgcgcgc cacccattgc atttcaccgt ggcgcctggc
     1921 gagaatcgta tcgtcggggt tatagacctg gatctggttg cgccccatgt ccttggcgac
     1981 ataacaggcc gcatcggcct gtttcagcag atcggtcacg ctcgccgtgg tctcgtcgat
     2041 cgccaccagg ccgatactga ccccgatctg aaacttttcg ccttcccaga agaactggaa
     2101 ttcctgaatg ttgcgctgc
//
LOCUS       DATCZV010000488         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_675496, whole genome shotgun sequence.
ACCESSION   DATCZV010000488 DATCZV010000000
VERSION     DATCZV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675496"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1893)
                     /locus_tag="VIW27_10995"
     CDS             complement(181..1893)
                     /locus_tag="VIW27_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5740244.1"
                     /translation="MSKPETKTDAVDKRQPRQRGKIKGRVVDLNALLEVQNLLGDAPR
                     VRDQLIEHLHKIQDRYGHLSAAHLVALAHEMRLSTTEVYEVASFYHHFDVVREGKTAP
                     PPLTVRVCDSVTCEMAGCEDLIAALEGSLGDGVRVQRVPCVGRCDQAPVAVVRQFTVD
                     NATADSVKAAVDGECFEQPLPEDCIDYDAYRAGGGYGLYERLLGGDIDAEEIMTTLEG
                     TQLRGLGGAGFPVGRKWRILRDQPAPRLCAINIDEGEPGTFKDRYYMLSDPHRFLEGM
                     LIAARVIDIESCYIYIRDEYPSVIEVLKRAVADLQAKSGYELPLIEIRRGAGAYICGE
                     ESAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNCESLFWIRDVVEKGPEWFDSQG
                     RNGRRGLRSFSVSGRVNQPGVHLAPAGITIKELIDEYCGGMQDGHRFYGYFPGGASGG
                     ILPASLGDIPLDFDTLQEYGCFIGSAAVVVLSEQDSARQAALNSLKFFAHESCGQCTP
                     CRVGCEKAVSIMESGDWDIEQLQDLSEVMIDASICGLGQAAPNPILSVIKYFPHELGI
                     EEDQ"
BASE COUNT          391 a          652 c          619 g          363 t
ORIGIN      
        1 gtatcagggt tggctgccat tatgagcacc ttaaaaaaca gtgatttttt cgtgagtgca
       61 aggaagcgcc gcgcgcagaa ccgcagtgta taggcgaata catgaggatt cgagtgggcg
      121 gcaccccgtc acggcgctga cgccgcaatc gcggaaaaag cgcattttta aaggagctca
      181 ttattgatcc tcctcgatac cgagttcatg ggggaagtac ttgatcaccg acaggatcgg
      241 gttaggcgcc gcctggccga gaccgcagat cgaggcgtcg atcatcacct cggaaaggtc
      301 ctgtaattgc tcgatgtccc agtcgccgct ctccatgatg ctgaccgctt tctcgcagcc
      361 gacgcggcac ggcgtgcact ggccgcagga ttcgtgggcg aagaacttta gcgagttcag
      421 tgccgcctgc ctggcgctgt cctgctccga cagcacgacc accgcggccg agccgatgaa
      481 gcaaccgtat tcctgcaggg tatcgaaatc tagcggaatg tctccaaggc tcgccggcag
      541 aatgccgccc gacgcaccgc cgggaaaata accgtaaaag cgatgcccgt cctgcatgcc
      601 gccgcaatac tcgtcgatca gttccttgat cgtgatgccg gccggcgcca ggtgcacacc
      661 gggctggttg acgcggccgg agaccgagaa actgcgcagg cccctgcggc cgttgcggcc
      721 ctgcgaatcg aaccactcgg gtcccttctc gaccacgtcg cgaatccaga acagcgattc
      781 gcaattatgc tcgagcgtcg gccggccgaa cagacccacc tgggcgacgt acggcggacg
      841 caaccgcggc atgccgcgct tgccctcgat cgactcgatc atcgccgatt cctcgccaca
      901 gatataggcg ccggcaccgc ggcgaatctc gatcagcggc aactcgtagc ccgatttcgc
      961 ctgcaaatcg gcaaccgcgc gtttcagcac ctcgatcacc gacgggtatt cgtcgcgaat
     1021 gtagatatag caggattcga tgtcgatgac ccgcgcggcg atcagcatgc cttcgagaaa
     1081 gcgatgcgga tcgctgagca tgtaatagcg atccttgaag gtccccggct cgccctcgtc
     1141 gatgttgatc gcacacagcc gcggcgccgg ctggtcgcgc agaatgcgcc atttgcggcc
     1201 aaccggaaaa ccggcgccgc cgagaccgcg caactgggtg ccttcgagcg tcgtcatgat
     1261 ttcctcggcg tcgatgtcac cgcctagcag gcgctcgtag agaccgtagc caccgccggc
     1321 acggtaggcg tcgtaatcga tgcaatcctc gggcagcggc tgctcgaagc actcgccgtc
     1381 gacggccgcc ttgacgctgt ccgcggtcgc gttatcgacc gtgaactgcc tgaccacggc
     1441 gaccggcgcc tggtcgcaac gcccgacaca gggcactcgc tgcacgcgca cgccgtcacc
     1501 gagcgaaccc tcgagggcgg cgataaggtc ctcgcaaccc gccatctcgc aggtgaccga
     1561 gtcgcagacg cgcaccgtca gcggcggcgg cgcggtcttg ccctcgcgca ccacgtcgaa
     1621 gtggtggtag aaggacgcga cctcgtagac ctcggtggtc gacaggcgca tttcatgcgc
     1681 cagcgccacc aggtgcgcgg ccgacagatg accgtagcgg tcctggattt tatgcaggtg
     1741 ttcgatcagc tgatcgcgga cgcgcggcgc atcgccgagc aggttctgca cctcgagcaa
     1801 tgcgttgaga tcgacgacgc ggcccttgat cttgccgcgc tggcgcggct gcctcttgtc
     1861 gaccgcgtca gttttcgttt cgggctttga catggcgtgc tctaccgcgt tgtcgatgga
     1921 caaaactccc cgccctgtcg ggcggggtaa agtttagccc aaggaggaga tgacaaaaac
     1981 atcggaactc gccattgcgc cgcgtcagtg cgccatcacg gtgat
//
LOCUS       DATCZV010000489         4953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_571353, whole genome shotgun sequence.
ACCESSION   DATCZV010000489 DATCZV010000000
VERSION     DATCZV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571353"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="mutL"
                     /locus_tag="VIW27_11000"
     CDS             complement(<1..857)
                     /gene="mutL"
                     /locus_tag="VIW27_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY5740245.1"
                     /translation="MASIRTLPSHLVNQIAAGEVVERPASVVKELIENSLDAGAGSIT
                     VEIDAGGTRLIRVSDDGQGIERDELASALSRHATSKIGSLEDLENIASLGFRGEALPS
                     IASVSRLSITSRQRDAERAWKLQGRDAGEPVPDPLQQGTRVEVLELFYNVPARKKFLR
                     TEQTEYKHIESLFKNLALSNPAVAFRLLHNQKTVYQLPSVQSDDDLRRRLAALCGSNF
                     ADSLVAIDVAADHLRLQGWVALPTFNRSQPDMQYFFVNRRMVRDKLVNHAVRQAYRDV
                     LFHGRHPAYV"
     gene            complement(866..1813)
                     /gene="tal"
                     /locus_tag="VIW27_11005"
     CDS             complement(866..1813)
                     /gene="tal"
                     /locus_tag="VIW27_11005"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY5740246.1"
                     /translation="MNKLEQLKSMTQVVADTGDLDAIARYQPQDATTNPSLLLKAVEL
                     PRYRPLLGAARRWGQQQGGDPHARAAAAGDYLAVSIGLEIQNIVPGRVSTEVDARLSF
                     DREATVARARRLIELYESRGASRDRVLIKIAATWEGIQAAHELESENINCNLTLLFGF
                     AQAVACAESGVYLVSPFVGRILDWHLAVSGNTAIPPQHDPGVQSVRRIYNYYKQHGFE
                     TVVMGASFRNTGQIEQLAGCDRLTISPQLMQQLEADEAHLPRLLGGDSGGAGEPRISI
                     DEKRFRWMMNEDAMTTEKLAEGIRLFTRDQEKLEALLVA"
     gene            1898..2671
                     /locus_tag="VIW27_11010"
     CDS             1898..2671
                     /locus_tag="VIW27_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5740247.1"
                     /translation="MTTEFTSIAALLAGIYLAVAAFLDFYQRRLIYVPVPHDAQFAAD
                     ELRIDNQGTRLHGWVLNPGQRSAVIYFGGNSEMISERREYFEDVFRDYSVYLFNYRGY
                     GNSEGRPTEVGLFSDALAIYDRIERQHQSIIAYGRSLGSGVAVHLAAERRLDKLILLT
                     PYDSIAAVGQQSYPVFPVYLLIRDRFDSAARAGEIRVPVMIAAAGRDRVIRRPRTLAL
                     KQAFRDAPLTYLSIAGAAHDDIVEFSEYREAVSRFLRRG"
     gene            complement(2836..3942)
                     /locus_tag="VIW27_11015"
     CDS             complement(2836..3942)
                     /locus_tag="VIW27_11015"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5740248.1"
                     /translation="MLSGAKPALARSRNALTGIRISESDAEHTRVVFDLSGSVEHRLF
                     TLADPHRVVIDLEDTHESDAIAIGSTRTTLMRGIRSASKENGRLRVVLDLQGKVRPRS
                     FLLKPDGKSGHRVVIDLHATNLSPTPIATSQQELHKRKSEYVIAIDPGHGGRDPGAIG
                     KKGTREKDVVLSVAQKMKDLVNRTPGYRAVLTREGDRFVSLRNRVKKAREAEADLFVS
                     LHADSFRSPAAKGASVYALSLSGASSEAARWIAQKENASDLIGGISLDDKDDLIASVL
                     LDLSQTATIQDSLDLGSDVLKHLGKVSRLNNSHVQQAGFAVLKAPDMPSILIETAFLS
                     NPSEERKLRNPKHQHKLARAVFSGIQHHLDNRQA"
     gene            complement(4136..4588)
                     /gene="tsaE"
                     /locus_tag="VIW27_11020"
     CDS             complement(4136..4588)
                     /gene="tsaE"
                     /locus_tag="VIW27_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013367945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY5740249.1"
                     /translation="MLIADEQRMRELGARLIAACDRGGVITLSGELGTGKTTLVRGAL
                     QAEGVVGGVRSPTYTLVEYYPLERFAVAHFDLYRLGDAEELEYLGFRDYLNPRTLCLI
                     EWPERAAGFLQVIDLEVGIEYDPAGRRVQLTAHSQLGRQWISRLRLPD"
BASE COUNT          880 a         1555 c         1561 g          957 t
ORIGIN      
        1 acgtaggccg ggtgccggcc gtgaaacagc acgtcgcggt aggcctggcg caccgcgtgg
       61 ttgaccagct tgtcgcggac catgcggcga ttgacgaaga aatactgcat gtccggctga
      121 ctgcgattga aggtcggcag cgccacccag ccctgcagcc gaaggtgatc ggcggcgacg
      181 tcgattgcca ccaggctgtc ggcgaagttc gagccgcaca gcgccgccag ccgccgccgc
      241 aggtcgtcgt ccgactgcac gctcggcagc tggtagacgg tcttctggtt gtgcagcagc
      301 ctgaaggcca cggccggatt cgacagcgcc aggttcttga acaggctctc gatatgcttg
      361 tactcggtct gctcggtgcg caggaatttc ttgcgtgccg gcacgttgta gaacaattcc
      421 agcacctcga cccgcgtgcc ctgttgcagc ggatccggga ccggttcgcc ggcatcgcgt
      481 ccctgcagct tccaggcacg ctcggcatcg cgctggcgcg aggttatgct gagccgggaa
      541 accgaggcaa tactcggcag ggcctcgccg cgaaacccca gcgaagcgat attttcgaga
      601 tcttcgaggc tgccgatctt actggtcgcg tggcgcgaca gcgcgctcgc gagttcgtcg
      661 cgctcaatcc cctggccgtc gtcgctgacc cggatcaggc gggtaccgcc ggcgtcgatc
      721 tcgaccgtga tcgagccagc gcctgcatcg aggctgtttt cgatgagttc cttgaccacc
      781 gatgccggcc gctcgaccac ctcgccagcg gcaatctggt tgaccaggtg ggatggcagg
      841 gttcgaatgc tggccaacgg aatactcagg cgaccagcag cgcttccagt ttctcctggt
      901 cgcgggtaaa caggcgaatg ccctcggcca gtttctcggt tgtcatcgcg tcctcgttca
      961 tcatccagcg aaagcgcttt tcgtcgatgc tgatgcgcgg ttcgccggca ccgccggagt
     1021 cgccaccgag gagtcgcggc aggtgcgcct cgtcggcctc gagctgctgc atcaattgcg
     1081 gactgatggt cagccggtca cagccggcga gctgttctat ctggccggta ttgcgaaagc
     1141 tcgcacccat caccacggtc tcgaagccgt gctgcttgta gtagttgtag atgcgcctca
     1201 ccgattgcac gcctggatcg tgctgcggcg gaatcgcggt attgccactc accgccagat
     1261 gccagtcgag gatgcggccg acgaacggcg agaccaggta gacgccgctt tcggcacagg
     1321 ccacggcctg cgcgaaaccg aacagcagcg tcaggttgca attgatattc tcactctcga
     1381 gttcgtgagc cgcctggata ccctcccagg tcgcggcgat cttgatcagc acgcgatcgc
     1441 ggctggcacc gcgactctcg tagagttcga tcagccgacg cgcccgcgcc acggtcgcct
     1501 cgcggtcgaa cgacaggcgc gcgtcgacct cggtggatac cctgccgggt acgatattct
     1561 ggatttcgag accgatgctg accgccaggt aatcgccggc ggcggccgcg cgcgcatgcg
     1621 ggtcaccgcc ctgctgctgc ccccagcgcc gtgccgcccc cagcaacggc cggtagcgcg
     1681 gcagctcgac cgccttcagc agcaacgacg gattggtggt cgcatcctgc ggctgatacc
     1741 tggcgatagc atccagatcg ccggtgtcgg cgaccacctg ggtcatggat ttgagctgct
     1801 ctagtttgtt cacgggtcga tgcttgctac tatatgcgcg cacattgttc gacgcgaaga
     1861 ataccagaaa atcccgcccg ggatgcttga atcatcgatg accacggaat tcacctcgat
     1921 agccgcgctg ctggccggga tctacctcgc tgtagcggct tttctcgatt tttaccagcg
     1981 ccggctgatc tatgttccgg taccccacga cgcgcagttc gccgccgacg aactgcgcat
     2041 cgataaccag ggtacgcggc tgcacggctg ggttttgaat cccggccagc gcagcgcggt
     2101 aatctatttc gggggcaatt cggaaatgat cagcgagcgc cgcgagtatt tcgaggacgt
     2161 gtttcgcgat tacagtgtct atctgttcaa ttatcgcggt tacggcaaca gtgaaggccg
     2221 gccgaccgag gtcggcctgt tttccgacgc gctggcgatc tacgatcgca tcgagcggca
     2281 gcatcagtcg atcatcgctt acgggcgcag cctcggcagc ggggtcgcag tgcacctcgc
     2341 ggccgagcgc cggctcgaca agctgatcct gttgaccccg tacgacagca tcgccgcggt
     2401 cggacaacag tcctacccgg tattcccggt gtacctgctg atccgcgacc gattcgactc
     2461 cgctgcgcgc gccggcgaga ttcgcgtgcc ggtgatgatc gcggccgccg gccgggatcg
     2521 cgtgatacgg cggccgcgca cgctggcact caagcaggca tttcgcgacg caccgctgac
     2581 ctacctatcg atcgccggcg ccgcgcacga cgatatcgtc gagttttccg agtaccgcga
     2641 agcggtcagc agattcctgc ggcgcggcta gcgcgcccat cacacgggca ttgcgcgctg
     2701 gcggcggaca gtcgtcgacg gcatggccgg ggcgtcgcca ggccgcatag atttttcaag
     2761 cgattgctgt gagcagccgc ggtacagcga tagccgctcg acgaggtcga ttcgactgcc
     2821 tgcagcggcg ccgcttcagg cctgccggtt atccaggtgg tgctgaatcc ccgagaacac
     2881 ggccctggcg agcttgtgct ggtgcttggg attgcgcagc ttgcgttcct cgctggggtt
     2941 ggacaaaaac gcggtttcga tcaggatcga cggcatatcg ggtgctttca acaccgcgaa
     3001 ccccgcctgc tgcacgtggc tgttatttag tcgcgacacc ttgccgagat gcttgagcac
     3061 gtccgatccc agatccaggc tgtcctggat ggtcgcggtc tgcgacaggt ccagcaacac
     3121 cgaagcgatc aggtcgtcct tgtcgtcgag gctgatgccg ccgatcaggt cggaggcatt
     3181 ttccttttgc gcgatccagc gcgcggcctc ggaactggcg ccggagagcg acagcgcgta
     3241 gaccgacgcc cccttggcgg ccgggctgcg aaaggaatcg gcgtgcagcg agacaaacag
     3301 gtcggcctcg gcctcgcgcg ccttcttgac gcgattgcgc aacgacacga agcgatcacc
     3361 ctcgcgggtc aggactgcac ggtatccggg cgtgcggttg accaggtcct tcatcttttg
     3421 cgccaccgac agcaccacgt ctttttcgcg ggtgcccttc ttgccgatgg cgcccgggtc
     3481 gcgtccaccg tgcccgggat cgatggcgat gacatattcg gacttgcgct tgtggagttc
     3541 ctgctggctg gtcgcgatcg gcgtcgggct caggttggtg gcatgcagat cgatcaccac
     3601 gcggtggccg gacttgccgt ccggcttgag caggaagctg cgcggacgca ccttgccttg
     3661 caggtcgagg accacgcgca ggcggccatt ttccttgctc gccgaacgaa tgccgcgcat
     3721 cagtgtggta cgcgtggagc cgatagcgat cgcatcgctt tcgtgggtat cttcgagatc
     3781 gatgaccacg cggtgcggat cggcgagcgt gaacaggcgg tgctcgacgc tgccgctgag
     3841 atcgaaaacg acccgcgtgt gctcggcgtc ggactccgag attcgaattc cggtcagcgc
     3901 gttcctggag cgcgccaggg ctggtttcgc gccgcttaac aacgccagcg acagccccag
     3961 tcgggcggcc cctgcgagga atcggcgtct ggctttgacg aatgggacca tgattactgt
     4021 tgttttgccc ttatccgcag ctatttatag taaaacaaag gacaaaaatc aacggttttt
     4081 ctatatttat taactagata ctgatgattt ctaaaaaata attcaagaaa tggggctagt
     4141 cgggcaagcg cagccgtgaa atccattgac ggccgagctg gctgtgcgcc gtaagctgca
     4201 cgcggcgtcc cgccgggtcg tactcgatcc cgacctcgag atcgatgacc tgcaggaaac
     4261 cggccgcgcg ctccggccac tcgatcaggc acagggttcg cgggttgaga tagtcgcgaa
     4321 agccgagata ctcgagttct tcggcgtcgc cgaggcggta gagatcaaaa tgcgccaccg
     4381 cgaagcgctc cagaggataa tactcgacca gcgtataggt cggactgcgc accccgccga
     4441 cgaccccctc ggcctgcagc gcgccgcgca ccagcgtggt cttgccggtg ccgagttcgc
     4501 cgctgagcgt gatcacgccg ccgcgatcac aggccgcgat cagccgcgcg ccgagttcac
     4561 gcatgcgttg ttcgtcagcg atcagcaagg ctagtccgca tattgcatgc agcaaacgaa
     4621 acccaggggt aatccggcca acgaggtcga aagctcggcc gccagcccac agccacttct
     4681 ttgggccaag gcggatcgct cgagattggc agccggattt ttcctggttt ggttcgaata
     4741 taaaccgcga cccgccctat tcgatatcat tacaacaagc ctccgcagtt ggtagtgttt
     4801 tcaagcgttg gtgacggctt cgcgcaatgc gcgggctaat ctcgaagttc gcgcatgacc
     4861 cgcggtatca ggtcgatgat gtcaccggcg acgaggccgg ccggatcggt gtcggcaacg
     4921 aagcgatcgg cacatagcgc gtgcaggaag acc
//
LOCUS       DATCZV010000490         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_252721, whole genome shotgun sequence.
ACCESSION   DATCZV010000490 DATCZV010000000
VERSION     DATCZV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252721"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1179)
                     /gene="serB"
                     /locus_tag="VIW27_11025"
     CDS             complement(253..1179)
                     /gene="serB"
                     /locus_tag="VIW27_11025"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY5740250.1"
                     /translation="MSDAPRAPGWLARADQPREFRLTLCGGRDAENSLAELRGALDGL
                     AMGVGWRPGELAWCLDVDCAPAEAWSVLQRLRERLADADVYLQPRDELGKRLLICDMD
                     MTIVAAETLDEVAASLGLGEKIASITRRAMHGELDFRAALRERIAMLAGQPEQVFHDI
                     VDKLELNPGAESLLAGARAAGVHTILVSGGFEQVARPVAERLGFDEVYCNRLEIDRGR
                     LTGGVPEPIVDAGLKRDILERQSAALGIPLAQCCAIGDGANDLPMLAAAGLGIAYRGK
                     PVLRDATPCRIDHSGLDAALHFMGLDLPGQGS"
     gene            complement(1176..2768)
                     /gene="serA"
                     /locus_tag="VIW27_11030"
     CDS             complement(1176..2768)
                     /gene="serA"
                     /locus_tag="VIW27_11030"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY5740251.1"
                     /translation="MDKPRVLISDSMSSQAVAVFEERGVDVVQSSKLSPEELNDMIGD
                     FDGLALRSATKVTAELLEHADRLKVVGRAGIGVDNVDVPACTKRGIVVMNTPLGNAIT
                     TAEHAMAMMLALARHIPQANRSTHDGKWEKSRFMGVELTGKLLGLIGAGNIGSIVAAK
                     AIGYGLHVQAYDPFLTEERADRLGVKKVDLDTLLSTSDIVSLHVPKTPETENIISASA
                     INKMKKGAMLINCARGGLVDELALKAALDSGHLRGAALDVYAVEPARDNPLFEIDNVI
                     CTPHLGASTIEAQEKVAIQIAEQISEFLLTGAVNNAINAPSISAEEAKALRPYLQLAR
                     NLGSFVGQLTRDPIRTLTITYGGEANDLNVSPLTVQVVQSVLEQHSSYVNSVNAREVA
                     RDRNIQVIEAHNEGRDEYPCRITVVVETESQSRSICGTLIQNQPRVIDVKGIPLESKL
                     GEHMLYITNYDKPGVIGDIGTSCSQRGINIANMHLGRDARDGNAIVLLEIDEPLDSEN
                     LEVLRGLPNINDVRYLHFPAMP"
     gene            complement(2761..>2938)
                     /locus_tag="VIW27_11035"
     CDS             complement(2761..>2938)
                     /locus_tag="VIW27_11035"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320562.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     and glutamate from O-phospho-L-serine and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine aminotransferase"
                     /protein_id="HEY5740252.1"
                     /translation="KRLDDEGAAYDIGNYRAAPPGFRLWGGATVETADLEALTPWLDW
                     VFADYQNSLEKNHG"
BASE COUNT          454 a          971 c          971 g          542 t
ORIGIN      
        1 gccgggcttg acccggcatc tccccatccc cgtcatgccg ggcttgaccc ggcatctccc
       61 catccccgtc atgccgggct tgacccggca tctcttcgtc ctcgtcatgc cgggcccgac
      121 ccggcatcca ttaccgatgc cgtccgtgcg ctgaagcccc ggcaagttat ttacgtttgc
      181 gaatgaacca ggttgccatg gaccccggct cgggggccgg ggtgacgaat tgccgcgtta
      241 aatgcgctgc ggctatgagc cctggccggg caaatcgagg cccatgaagt ggagcgcggc
      301 gtcgaggccg ctgtgatcga tgcggcacgg agtcgcgtcg cgcaacaccg gcttgccgcg
      361 gtaggcgatg ccgagcccgg cagcagcgag catcggcagg tcgttggcgc cgtcgccaat
      421 cgcgcagcat tgcgcgagcg gtatgccgag cgccgcggac tgccgctcga ggatatcgcg
      481 cttcagccca gcgtcgacga tcggttccgg cacgccgccg gtgagcctgc cgcggtcgat
      541 ctcgaggcgg ttgcaataca cctcgtcgaa gccgaggcgc tcggcgaccg gccgcgcgac
      601 ctgctcgaag ccaccgctga ccaggatagt atgcacaccg gcggcccgag cacccgcgag
      661 cagcgactcc gcgccggggt tcagttcaag tttgtcgaca atgtcatgga atacctgttc
      721 cggctgcccg gcgagcatcg caatgcgctc gcgcagcgcg gccctgaaat cgagttcgcc
      781 gtgcatcgcg cgccgggtga tcgaggcgat tttttcgccg aggccgagcg aggccgcgac
      841 ctcgtcgagg gtttcggcgg cgacgatggt catgtccatg tcgcagatca acagccgctt
      901 gccgagttcg tcgcgcggct gcaggtagac gtcggcgtcg gccaggcgct cacgcaggcg
      961 ctgcagcaca gaccaggcct cggccggcgc gcaatcgaca tcgagacacc aggcgagttc
     1021 gcccgggcgc caaccgacac ccatcgccag gccgtcgagc gcgccgcgta attcggcgag
     1081 cgagttctcg gcatcgcgcc ctccgcacag tgtgagccgg aactcgcgcg gctggtcggc
     1141 gcgcgcgagc cagccgggtg cccgcggcgc gtcgctcatg gcatcgccgg aaagtgcagg
     1201 tagcgcacgt cgttgatgtt cggcagaccg cgcagcacct cgagattttc gctgtcgagc
     1261 ggctcgtcga tttccagcag tacgatggcg ttaccgtcac gcgcgtcgcg gccgaggtgc
     1321 atgttggcga tgttgatgcc gcgctgcgag caactggtgc cgatgtcgcc gataacgccc
     1381 ggcttgtcgt agttggtgat atagagcatg tgctctccca gcttggactc cagcggaata
     1441 cccttgacgt cgatcacccg cggctggttc tggatcagcg tgccgcagat gctgcgcgac
     1501 tggctctcgg tctcgacgac gaccgtgatg cggcatgggt actcgtcgcg gccctcgttg
     1561 tgcgcctcga ttacctggat gttgcgatca cgcgcgacct cgcgcgcgtt gaccgagttg
     1621 acgtagctcg aatgctgctc gagcaccgac tgcaccacct gcacggtcag cggcgagacg
     1681 ttgagatcgt tggcctcgcc accgtaggtg atcgtcagcg tcctgatcgg gtcgcgcgtc
     1741 agctgaccga cgaacgaccc caggttgcgc gccagttgca ggtaggggcg cagcgccttg
     1801 gcttcctcgg cgctgatact gggcgcgttg atcgcattat tgaccgcgcc agtgagcagg
     1861 aattcgctga tttgctcggc gatctggatg gcaacctttt cctgcgcctc gatcgtcgac
     1921 gcgccgaggt gcggcgtgca gatcacattg tcgatctcga acagcggatt gtcgcgcgcc
     1981 ggctccaccg cgtagacatc gagtgcggcg ccgcgcaggt ggccgctgtc gagcgccgcc
     2041 ttcagcgcca gctcgtcgac cagcccgccg cgcgcgcagt tgatcagcat cgcgcccttc
     2101 ttcatcttgt tgatcgcgct ggcgctgatg atgttctcgg tctccggcgt cttcggcacg
     2161 tgcagcgaaa cgatgtcgga agtcgatagc agcgtatcga gatcgacttt tttcacgccg
     2221 aggcggtcgg cgcgctcctc ggtcaggaac ggatcgtagg cctgcacgtg caagccgtaa
     2281 ccgatcgcct tggcggcgac gatcgagccg atgttgccgg cgccgatcag gccgagcagc
     2341 ttgccggtca gctcgacgcc catgaaacgc gacttttccc acttgccgtc gtgggtcgag
     2401 cggttggcct gcggaatatg ccgggccagc gccagcatca tcgccatcgc atgctcggcg
     2461 gtggtgatcg cgttgccgag cggcgtgttc atgaccacga tgccgcgctt cgtgcaggcc
     2521 ggcacgtcga cgttgtcgac gccgatgccg gcgcggccga ccaccttgag ccggtcggcg
     2581 tgctcgagca attcggcggt aaccttggtc gccgagcgca gcgcgagacc gtcgaagtcg
     2641 ccgatcatgt cgttcaactc ctcgggcgag agcttgctcg actgcaccac gtcgacgccg
     2701 cgttcctcga acaccgcgac cgcctggctc gacatcgagt cggaaatcag cactctgggt
     2761 ttatccatga tttttctcca ggctgttttg gtagtcggca aagacccagt cgagccacgg
     2821 cgtcagtgcc tcgagatcag cggtttcgac cgtcgctccg ccccacaggc gaaagccggg
     2881 cggcgcggcg cggtagttgc cgatgtcgta ggcggcgccc tcgtcgtcga ggcgcttg
//
LOCUS       DATCZV010000491         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_93004, whole genome shotgun sequence.
ACCESSION   DATCZV010000491 DATCZV010000000
VERSION     DATCZV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93004"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VIW27_11040"
     CDS             <1..185
                     /locus_tag="VIW27_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY5740253.1"
                     /translation="GKNVLNFFRQDHGYKEGSYVKQWHGREDNEHLSELLSQLDSGSA
                     DFREQVYRGLEQRYPG"
     gene            complement(210..1460)
                     /locus_tag="VIW27_11045"
     CDS             complement(210..1460)
                     /locus_tag="VIW27_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY5740254.1"
                     /translation="MDHATIYIVLAGALGLFMAWGIGANDVANAMGTSIGSRVLTIRK
                     AVLVAAVFEFAGAFLAGGQVTSTIRKGIIDSAAVAQSPDLLIFGMLAALTAAGIWLVI
                     ASRNGWPVSTTHTIVGAIVGFAIAGLGVEAVKWGKVGNIVASWVVSPVIAGALAFFLF
                     RSVQRLILNTDDPIANAKRYVPFYIFVTGFMIALVTLFKGLKHIGLEFSGWQNCLIAA
                     AVGLLVMVCGIFFISRIKIDPADDKDFHFANVEKIFAVLMVVTACSIAFAHGSNDVAN
                     AVGPMAAIISVASEGVVSQKSPMPIWVLLVGGVGIVIGLATYGKRVIATIGRKITELT
                     PSRGFACELAASTTIVIASGTGLPISTTHTLVGAVLGVGMARGIGALNLRVVGNIFMS
                     WIVTLPAGALLSIFFFYFFKGIFG"
     gene            complement(1494..2171)
                     /locus_tag="VIW27_11050"
     CDS             complement(1494..2171)
                     /locus_tag="VIW27_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HEY5740255.1"
                     /translation="MPKNYFSRIFSQSPFKAMQEHMAIVEECVHQLGPFFKAAIKGDQ
                     DKVKQLYKQISKLENRADTLKRNLRLHMPKGLFMPVARRDLLELLLAQDQAANQAKDI
                     SGLVTGRKMEFPEKLARLLPDFVARCEDACRQANKVINELDELVETGFAGKEVKLVES
                     IINELDSIEKDTDKMQIKIRAALLKIEKDLPPVDVMFYYRVIEGIGKVADISQRIGSR
                     VELLLAK"
     gene            complement(2325..3029)
                     /gene="phoU"
                     /locus_tag="VIW27_11055"
     CDS             complement(2325..3029)
                     /gene="phoU"
                     /locus_tag="VIW27_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY5740256.1"
                     /translation="MTEPQITGHISKRFDQELEDIHNKVLSMGGLVENQVANGIRSLV
                     QSDSELARKVIADDHKVNRMEVEIDEECVHILARRQPAAGDLRLIVAVIKTITDLERI
                     GDQAEKLGRIQLELEQQEISSSAYIKLEHLGELVAKILHSTLDAFARMNVADAMATME
                     LDNRVNDEYDGLMREMITHMMEDPRTIRSSLRISWCARALERIGDHAKNICEYVVFLV
                     QGKDVRHITPKDQPGL"
     gene            complement(3034..3858)
                     /gene="pstB"
                     /locus_tag="VIW27_11060"
     CDS             complement(3034..3858)
                     /gene="pstB"
                     /locus_tag="VIW27_11060"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY5740257.1"
                     /translation="MKAEKASASQQDFTGTFGEIQVDNPKISTRNVNVFYGDKQAIFD
                     ASLDIGRNEVIALIGPSGCGKSTYLRALNRMNDTIIGCRVEGQLLLDGEDIYAKSMDP
                     VLLRARVGMVFQKPNPFPKSIYDNIAYGPRIHGLARNNAEIDEIVESSLKRAGLFEEV
                     KDRLQSPGTGLSGGQQQRLCIARAIAVDPEVILMDEPASALDPIATAVIEELIDELRA
                     SYTIVIVTHSMQQAARVSQRTAYFHLGKLIEVGETSQIFTNPRHELTENYITGKIG"
     gene            complement(3941..>4584)
                     /locus_tag="VIW27_11065"
     CDS             complement(3941..>4584)
                     /locus_tag="VIW27_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PstA family ABC transporter permease"
                     /protein_id="HEY5740258.1"
                     /translation="TLLVTLIVSLPVGVSAAIYLEEFAPQNRLTDFIEININNLAAVP
                     SIVFGLLGLAVFINFFGMPRSAPIVGGLVLSLMTLPTIIISSRAALKSVPPSIKEAAL
                     GIGASHMQVVFHHTLPLAMPGILTGSIIGMAQALGESAPLLMIGMVAFIVDVPGGIFD
                     SATVLPVQIYLWSDSPERAFTALTAATILVLLAFLTAMNAIAIYLRRRFERRW"
BASE COUNT          884 a         1380 c         1406 g          914 t
ORIGIN      
        1 tcggcaagaa cgtgctcaac ttttttcgcc aggatcatgg ctacaaggag ggcagctacg
       61 tcaagcagtg gcacgggcgt gaggataacg agcacctcag cgagctgcta tcgcagctcg
      121 atagcggcag cgccgatttt cgcgagcagg tctaccgcgg tctcgagcag cgctatccgg
      181 gctgacggac ttcagcccgc ggctgcggtc tagccgaaga ttcccttgaa gaagtaaaag
      241 aaaaagatcg acagcaacgc gccggccggc agggtcacga tccaggacat gaaaatattg
      301 ccgaccacgc gcaggttgag cgcgccgata ccgcgcgcca tgccgactcc gagtaccgcg
      361 ccgaccagcg tatgcgtggt cgatatcggc aggccggtgc ccgaggcgat gacgatcgtc
      421 gtcgacgcgg cgagttcgca ggcaaagccg cggctcggcg tcagttcggt gatttttcgt
      481 ccgatagtgg cgattacgcg cttgccgtag gtcgccagcc cgatgacgat gccgacgcca
      541 ccgaccagca gcacccagat cggcatcggc gatttctgtg aaaccacgcc ttcgctggcg
      601 acgctgatga tcgcggccat cggcccgacc gcgttggcga cgtcgttgga gccgtgggcg
      661 aaggcgatcg aacaggcggt gaccaccatc agcacggcga aaatcttttc cacgttggcg
      721 aaatggaagt ccttgtcgtc ggccggatcg atcttgattc gcgagatgaa gaagatgccg
      781 cagaccatca ccagcagccc gacggcggcc gcgatcaggc agttctgcca gccggaaaac
      841 tcgaggccga tgtgtttgag gcccttgaac agggtaacca gcgcgatcat gaagccggtg
      901 acgaagatgt agaacggcac gtaacgcttg gcgttggcga tcgggtcgtc ggtgttcaga
      961 atcagccgct gcacgctgcg gaacaggaaa aacgccagcg ccccggcgat taccggcgat
     1021 accacccagc tggcgacgat attgccgacc ttgccccact tcaccgcctc gacgccgagt
     1081 ccggcgatcg cgaagccgac gatcgcgccg acgatggtat gcgtggtcga caccggccag
     1141 ccgttgcgcg aagcgatcac cagccagatg ccggccgcgg tcagcgccgc cagcatgccg
     1201 aagatcagca ggtccgggga ttgcgcgacc gcggcgctgt cgatgatacc cttgcgaatc
     1261 gtcgaggtga cctgtccgcc ggcgagaaac gcgccggcga attcgaacac cgccgcgacc
     1321 agcacggcct tgcgaatcgt cagcacccgc gagccgatcg acgtgcccat cgcattggcg
     1381 acgtcgttgg cgccgatccc ccaggccatg aacaggccga gcgcaccggc gagcacgatg
     1441 tagatggtgg cgtggtccat gccttgaggc tgcgcgaaac ccggcggccg cgcttacttc
     1501 gccagcagca gctcgacgcg gctgccgatg cgctgggaga tatcggcgac cttgccgata
     1561 ccctcgatga cccggtaata aaacatcaca tcgaccggcg gcaggtcctt ttcgatcttc
     1621 agcaacgcgg cgcgaatctt gatctgcatc ttgtcggtat ccttctcgat cgagtccagc
     1681 tcgttgatga tcgactcgac gagcttgact tccttgccgg cgaagccggt ctcgaccagc
     1741 tcgtcgagtt cgttgatgac cttgttggcc tggcggcagg cgtcctcgca gcgggccacg
     1801 aagtccggca acagccgtgc gagcttttcg gggaactcca tcttgcgccc ggtgaccagt
     1861 ccgctgatat ccttggcctg gttggccgcc tggtcctgcg ccagcagcaa ctccagcagg
     1921 tcgcgccgcg cgaccggcat gaacaggccc ttgggcatat gcaggcgcag gtttctcttc
     1981 agcgtatcgg cccggttctc gagcttgctg atctgcttgt agagttgttt gaccttgtcc
     2041 tggtcgccct tgatcgcggc cttgaagaac gggcccagct ggtgcacgca ctcttcgacg
     2101 atcgccatgt gctcctgcat cgccttgaac ggagactggc tgaagatcct ggagaaataa
     2161 ttcttcggca tcgctatatc cgcagacggg ataccgcgaa ttgtgctgat ctaagactca
     2221 atagcaatcg tttaattcaa tagctagcct taaattatgc cggctgggct cgccactgaa
     2281 cctcgccggc gcgcgctttc ggtaagcggc gggaaatcgc ggaattacag gcccggctga
     2341 tccttcggcg tgatatgcct gacatccttg ccctgcacca gaaataccac gtactcgcag
     2401 atgttcttgg catgatcgcc gattcgttcg agtgcgcgcg cgcaccagga gatgcgcagc
     2461 gagctgcgga tggtgcgcgg atcttccatc atgtgggtga tcatttcgcg catcaggccg
     2521 tcgtactcgt cgttgacccg gttatccagt tccatggtcg ccatggcgtc ggccacgttc
     2581 atgcgtgcaa acgcatccag cgtgctgtgc aggatcttgg cgaccagttc gccgaggtgc
     2641 tcgagcttga tatatgccga cgaggaaatt tcctgctgtt ccagctcgag ctgaatgcgc
     2701 cccagtttct cggcctggtc gccgatgcgc tcaaggtcgg ttatcgtctt gatgaccgct
     2761 acgatcagcc gcaggtcgcc ggccgccggt tgccgccgcg ccagaatgtg cacgcattcc
     2821 tcgtcgatct cgacttccat gcggtttacc ttgtggtcat cggcgattac tttgcgggcc
     2881 agttccgaat ccgactgcac caggctcctg atgccgttgg cgacctggtt ttcgaccagc
     2941 ccgcccatcg acagcacctt gttgtgaatg tcttcgagtt cctggtcgaa gcgctttgaa
     3001 atatgtccgg taatctgcgg ctcggtcatg gcgttatccg atttttccgg tgatgtaatt
     3061 ttccgtcaac tcgtgccgcg gattggtaaa gatctggctg gtttcgccga cttcgatcag
     3121 cttgccgagg tgaaaatagg cggtgcgctg cgaaacccgc gccgcctgct gcatcgagtg
     3181 agtgacgatg acaatcgtgt agctggcgcg taactcgtcg ataagttcct cgatcaccgc
     3241 ggtggcgatc gggtcgagcg ccgaagccgg ttcgtccatc aggatgacct ccgggtcgac
     3301 cgcgatcgca cgcgcgatac acaggcgctg ttgctggccg cccgacagcc cggtgccagg
     3361 cgactgcagg cgatccttga cttcttcgaa caggcccgcg cgcttgaggc tggattcgac
     3421 gatttcgtca atctcggcat tgttcctggc gagaccgtga atgcgcggtc cgtaggcgat
     3481 gttgtcgtag atcgacttcg gaaacggatt gggtttctgg aaaaccatgc cgacgcgcgc
     3541 gcgcagcaac accggatcca tactcttggc gtaaatgtct tcaccgtcga ggagcagttg
     3601 cccctcgacc cggcaaccga tgatggtgtc gttcatgcga ttcagggccc gcaaataggt
     3661 cgatttgccg caacccgacg ggccgatcag cgcgatgacc tcgttgcgac cgatgtccag
     3721 cgaggcatcg aaaatcgcct gtttatcgcc atagaagacg ttgacgttgc gcgtcgagat
     3781 ctttggattg tcgacctgta tttcaccaaa ggtgccggtg aaatcctgct ggcttgcgga
     3841 ggctttttct gctttcaaat cgagtggcgc gacgggcgcg ctgtctgcta cgttttcagt
     3901 aaccgaaaaa ctcatattat cagactcttg gctgcgtgtg ttaccagcgg cgctcgaacc
     3961 tgcggcgcag gtaaatggcg atggcattca tcgcggtcag gaatgccagc agcaccagga
     4021 tggtggctgc ggtgagcgcg gtaaacgcgc gctcggggct gtccgaccac aggtaaatct
     4081 gtaccggcaa cacggtcgcg gagtcgaaga tgccgcccgg cacgtcgacg ataaaggcaa
     4141 ccatgccgat catcagcagc ggcgcggatt cgcccagcgc ctgcgccatg ccgatgatcg
     4201 agccggtgag gatgccgggc attgccagcg gcagcgtatg gtggaagacg acctgcatgt
     4261 gcgaggcgcc gatgccgagc gcggcttcct tgatcgacgg cggtaccgat ttgagtgccg
     4321 cccggctgga gatgatgatc gtcggcagcg tcatcagcga taacaccagg ccgccgacga
     4381 tcggcgccga acgcggcatg ccgaagaaat tgatgaacac ggcgagaccc agcaggccga
     4441 agacgatcga cggcaccgcg gccaggttat tgatattgat ttcgatgaaa tcggtcaacc
     4501 tgttttgcgg cgcgaattcc tcgaggtaga tcgccgccga gacgcccacc ggcagcgaca
     4561 cgatcaacgt taccagcagc gtcc
//
LOCUS       DATCZV010000492         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_729083, whole genome shotgun sequence.
ACCESSION   DATCZV010000492 DATCZV010000000
VERSION     DATCZV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729083"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VIW27_11070"
     CDS             <1..192
                     /locus_tag="VIW27_11070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY5740259.1"
                     /translation="GIGWLFGKTAVFMLLFLWFRATFPRYRYDQIMRLGWKVLLPVTI
                     VWLAVEGLAVYFKIGPWFA"
     gene            197..688
                     /gene="nuoI"
                     /locus_tag="VIW27_11075"
     CDS             197..688
                     /gene="nuoI"
                     /locus_tag="VIW27_11075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY5740260.1"
                     /translation="MFAKIQYYLKSFLFLELLQGMSVTGRYFFRKKFTIQYPEQKTPI
                     SPRFRGLHALRRYANGEERCIACKLCEAVCPALAITIESEQRADGTRRTTRYDIDLFK
                     CIYCGFCEEACPVDSIVETNIFEYHFENRGENIMTKQKLLEIGDRYEAQLAADRSADA
                     RYR"
     gene            694..1293
                     /locus_tag="VIW27_11080"
     CDS             694..1293
                     /locus_tag="VIW27_11080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5740261.1"
                     /translation="MVETVIFYVFAVATVISATSVVTVRNPVHAALFLVLTFFTSAAI
                     WLMLEAEFLAITLVLVYVGAVMVLFIFVVMMLDINIARLREGFVRYLPLGLLVAAIML
                     GMMLSVVTSDTFVLAAGSEPPPKPADFSNTKALGSVLYTEYVFAFEVAALILLVAIIA
                     AISLTMRKRSQTKYQDASSQIKVRKADRLRIVKMPASEE"
     gene            1299..1604
                     /gene="nuoK"
                     /locus_tag="VIW27_11085"
     CDS             1299..1604
                     /gene="nuoK"
                     /locus_tag="VIW27_11085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5740262.1"
                     /translation="MVTLSHYLILAAVLFSISIAGIVINRKNIIILLMSIELMLLSVN
                     FNFIAFSHYLDDLAGQVFVFFILTVAAAEAAIGLAILVVLFRNKRSINVEDIKELKW"
     gene            1607..>2560
                     /locus_tag="VIW27_11090"
     CDS             1607..>2560
                     /locus_tag="VIW27_11090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5740263.1"
                     /translation="MENVYLAIPLSSLAGALIAGLFGKQIGRAGAHWVTIIGVAISFI
                     LSLVVLKDVAIDGAPIYNQAVYTWMYSDGILFQVGFLIDGLTALMMAVVTGVSLAVHV
                     YTIGYMHDDDGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWFK
                     RETAIYANLKAFLVNRVGDFGFLLGIAVILMYTGSLDYYEVFDRSGAMLERTLEVIPG
                     AEWSVITLACILLFIGAMGKSAQMPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVA
                     RMSPLFELSTTALGFVIIIGAVTAFFMGLLGIVQNDIKRVVA"
BASE COUNT          450 a          765 c          755 g          590 t
ORIGIN      
        1 ggtatcggct ggctgttcgg caagaccgcg gtattcatgc tgctgttcct gtggtttcgc
       61 gcgactttcc cgcgttaccg ctacgaccag atcatgcggc tcggctggaa ggtattgctg
      121 ccggtgacta tcgtctggct cgcggtcgag ggactcgcgg tttacttcaa aatcggcccc
      181 tggttcgcgt aaagtcatgt ttgccaagat acagtactac ctgaagagtt ttctgttcct
      241 cgaactgctg cagggcatga gcgtcaccgg gcgttatttc ttccgcaaga agttcaccat
      301 ccagtacccc gagcagaaaa ctccgatcag cccgcggttt cgtggtttgc acgcgctccg
      361 ccgctatgcc aacggcgagg agcgctgcat tgcctgcaag ctctgcgagg cggtctgccc
      421 ggcgctggcg atcaccatcg agtccgagca gcgagccgat ggcacgcgcc gcaccacgcg
      481 ctacgatatc gacctgttca agtgcatcta ctgcggcttt tgcgaggaag cctgcccggt
      541 cgattcgatc gtcgaaacca atattttcga gtatcacttc gaaaaccgcg gcgaaaacat
      601 catgaccaag caaaaactgc tggagatcgg cgatcgttac gaggcgcaac tggccgccga
      661 tcgatcggcg gatgcaaggt accggtaaac gatatggtcg aaaccgtcat attttacgtc
      721 tttgccgtcg ccacggtgat cagcgcgacc tcggtggtaa cggtcaggaa cccggtgcac
      781 gccgcgttgt tcctggtgct gacgtttttc accagtgccg cgatctggct gatgctggag
      841 gccgaattcc tggcgatcac cctggtgctg gtctatgtcg gcgcggtaat ggtgctgttc
      901 atcttcgtgg tgatgatgct ggatatcaac atcgcgcgcc tgcgcgaggg cttcgtgcgt
      961 tacctgccgc tcggcctgct ggtagcggcg atcatgctcg gcatgatgct gagcgtggtc
     1021 accagcgata ccttcgtgct tgcagcaggc agcgagccgc cgcccaagcc cgccgatttc
     1081 agcaatacca aggcgctcgg cagcgtgctc tacaccgagt acgtgttcgc cttcgaggtc
     1141 gccgcgttga tcctgctggt ggcgatcatc gccgccattt cgctgaccat gcgcaagcgt
     1201 tcgcagacca agtaccagga tgcgtcgtcg cagatcaagg tgcgcaaggc ggaccgcttg
     1261 cggatcgtca aaatgccggc cagcgaggag tgacgaacat ggtgacgctc tcccattacc
     1321 tgattctcgc cgcggtgctg ttcagcatct cgatcgccgg catcgtcatc aaccgcaaga
     1381 acatcatcat cctgctgatg tcgatcgaat tgatgctgtt gtctgtaaat ttcaatttca
     1441 tcgccttttc gcattacctc gacgatctcg ccgggcaggt gttcgtgttc tttatcctca
     1501 cggtagccgc cgccgaggcc gcgatcgggc tcgccatcct ggtggtgctg ttccgtaaca
     1561 agcgctcgat caatgtcgaa gatatcaagg agctgaaatg gtgagcatgg aaaacgtcta
     1621 tctcgccatt ccgctgtcgt cgctggccgg cgcactgatc gccggcctgt tcggcaagca
     1681 gatcggccgc gccggcgcgc actgggtgac gatcatcggc gtggcgattt cgttcatatt
     1741 gtcgctggtg gtgctcaagg acgtcgccat cgacggcgca ccgatctaca accaggcggt
     1801 ctacacctgg atgtacagcg acggcatcct gttccaggtc ggcttcctga tcgacggcct
     1861 gaccgcactg atgatggcgg tggttaccgg cgtatcgctc gcggtgcacg tctacaccat
     1921 cggctacatg cacgacgacg acggctacca gcggttcttc agctacatct cgctgttcac
     1981 cttctcgatg ctgatgctgg tcatgtcgaa caacttcctg cagctgttct tcggctggga
     2041 agcggttggc ctggtgtcct atttgctgat cggcttctgg ttcaaacgcg agaccgcgat
     2101 ctacgccaat ctgaaggcct tcctcgtcaa ccgcgtcggc gatttcggct ttctgctcgg
     2161 tatcgcggtg atcctgatgt acaccggctc gctcgactac tacgaggtgt tcgaccgttc
     2221 cggcgcgatg ctcgagcgca cgctggaagt gattcccggt gccgagtggt cggtgatcac
     2281 gctggcctgc atcctgttgt tcatcggcgc gatgggcaag tcggcgcaga tgccgctgca
     2341 cgtctggctg cccgattcga tggaaggccc gacgccgatt tctgcactca tccacgcggc
     2401 gacgatggtc acggcaggca tctttatggt ggcgcgcatg tcaccgctgt tcgagctgtc
     2461 gacgacggcg ctcggcttcg tgatcatcat cggcgccgtc acggcgtttt tcatgggcct
     2521 gctgggcatc gtgcagaacg acatcaagcg cgttgtcgcc
//
LOCUS       DATCZV010000493         3979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_146531, whole genome shotgun sequence.
ACCESSION   DATCZV010000493 DATCZV010000000
VERSION     DATCZV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146531"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VIW27_11095"
     CDS             complement(<1..623)
                     /locus_tag="VIW27_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5740264.1"
                     /translation="MLSLDDIIVVSLEQAVAAPFCSNRLAHAGARVIKIEREEGDFAR
                     GYDSAVHGESAYFIWLNQGKESLVMDIKNAADAELLHRILGRADVFVQNLAPGAAARA
                     GFGSEELRQLYPRLICCDISGYGDSGEYSEMKAYDLLVQCESGMASITGTPEAPGRIG
                     VSACDINCGQQAYAAILEALIARERSGSGAIIKVSLFDAMAEWLAVPL"
     gene            complement(623..1780)
                     /locus_tag="VIW27_11100"
     CDS             complement(623..1780)
                     /locus_tag="VIW27_11100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5740265.1"
                     /translation="MSEDRSYPEVRDAVARLCVDYPDEYWRDKDRDKAYPEEFVQALT
                     EAGYLSVLIPEEYGGAGMDLAAACAVLEEIQRSGANGAACHAQMYIMGSLLRHGSEAQ
                     KQKYLPDIASGKLRLQAFGVTEPTSGTDTTRIKTRARRDGDDYVVSGQKVFISRVEHS
                     DLLLLLVRTTPRDEVDKPTRGMSVLLVDLREAVGNGLTVRPLETMLNHATTELFFDQL
                     RVPAENLIGEEGKGFGYILDGMNAERILIAAECIGDARWFVDRASSYAREREVFGAAI
                     GKNQGIQFPIARAHVQTEAAALMVDKACGMFDAGLACGSEANMAKLLAADASWAAADM
                     CLQTHGGYGFTVEYDIERKFRETRLYQVAPISTNLILSNIATHVLGLPKSF"
     gene            complement(1857..3074)
                     /locus_tag="VIW27_11105"
     CDS             complement(1857..3074)
                     /locus_tag="VIW27_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY5740266.1"
                     /translation="MSQVQRPEFFELHNGSKAKLPFSDAEYETRLGALRELMASSGID
                     AMLLTSMHNIAYYSGFLYCSFGRPYACVVTAERCVTVSANIDLGQPWRRGFGDNLIYT
                     DWQRNNYWRAVRSIIPQARRVGIEGDHMTLAARELRSEFLGDAEACDMNREIMGLRMI
                     KSVEEIELIKGGARTADIGGEAIRDAIRVGAREIDVAMAGRDAMELEIARAYPDSEIR
                     DSWVWFQSGINTDGAHNPVTTRALQAGDILSLNTFPMISGYYTALERTLFIGEPDTDS
                     LRIWQANVGAHELGLSLIKPGATCSGICAEINRYFADLDLLQYRTFGYGHSFGVLSHY
                     YGREAGLELREDIDTVLEPGMVVSMEPMLTIPEGLPGAGGYREHDILVIGEQGAENIT
                     GFPSGPEHNIISA"
     gene            3264..3737
                     /locus_tag="VIW27_11110"
     CDS             3264..3737
                     /locus_tag="VIW27_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010141238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5740267.1"
                     /translation="MKSTTYQIRPATAADRAQWDPLWQGYLGFYETTLAPEVTDLLWQ
                     RILDPAHEIECRLAAAADASLAGLVHYFPHAHTWYANPVCYLNDLFVLPGIRGGGIGK
                     LLIEAVADEAKQRGWSEVYWHTQQHNTVARGLYDKLTGGTDGFVNYTIEVTARET"
BASE COUNT          843 a         1218 c         1143 g          775 t
ORIGIN      
        1 agcggtaccg ccagccactc ggccatggcg tcgaacaggg agaccttgat aatggcgcca
       61 ctgccgcttc gttcgcgcgc gatcagggct tcgagaatcg cggcataggc ctgctgcccg
      121 cagttgatat cgcaggccga aacgccgatg cgcccgggcg cttccggcgt gccggtgatc
      181 gatgccatgc cggattcgca ctgcaccagt aaatcatagg ccttcatctc gctgtattcg
      241 ccgctgtcgc cgtaacccga tatatcgcaa cagatcagtc gcggataaag ttggcgcagt
      301 tcctcgctgc cgaacccggc gcgtgcggcc gcacccgggg ccaggttctg gacaaacacg
      361 tcggcccggc cgagtatccg gtgtaatagt tccgcgtcgg ccgcattttt gatatccatc
      421 accagcgatt ccttgccctg gttgagccag ataaagtagg ccgactcacc gtgcaccgcg
      481 ctgtcgtaac cgcgtgcgaa atcgccctct tcgcgctcga tcttgattac tcgcgcgccg
      541 gcgtgtgcca gccgattgga acagaaagga gcggccaccg cctgctccag cgaaaccacg
      601 atgatgtcgt caagactgag cattaaaaag acttgggcag gcccagcacg tgggtcgcaa
      661 tattcgacag gatcaggtta gtggatatcg gcgccacctg gtacaggcgg gtttcacgaa
      721 acttgcgctc gatatcgtat tcgacggtaa agccgtaacc gccgtgggtt tgcaggcaca
      781 tatccgccgc ggcccaggag gcatcggcgg ccagcagctt ggccatattc gcttccgagc
      841 cacaggccag gcccgcgtcg aacataccgc aggccttgtc gaccatcagc gcggcggcct
      901 cggtttgcac atgggcgcgc gcaatcggaa actggatacc ctggttcttg ccgatcgcgg
      961 cgccaaacac ctctcgctcg cgcgcgtagc tgctggcgcg atcgacgaac cagcgcgcat
     1021 cgccgataca ctcggcggcg atcaggatac gttccgcatt cataccatcg agaatgtagc
     1081 cgaagccctt accttcctcg ccgatcaggt tttccgccgg cacccgcagt tgatcgaaaa
     1141 ataactcggt ggtggcgtga ttgagcatcg tttccagcgg cctgacggtc aggccattgc
     1201 cgacagcctc gcgcaaatcc accagcaaca ccgacatgcc gcgggtcggt ttgtcgacct
     1261 cgtcacgcgg cgtggtgcgc accagcagca acagcaggtc ggagtgctcg acacgcgaga
     1321 tgaacacctt ttgcccgctg acgacataat cgtcgccatc gcgccgcgcg cgcgtcttga
     1381 tgcgcgtggt atcggtgccg ctagtgggtt cggtcacacc gaaggcctgc aaccgtaatt
     1441 tgcccgacgc gatatcgggc agatacttct gtttttgcgc ctcgctgccg tgacgcagca
     1501 ggctgcccat gatgtacatc tgcgcgtggc aggcggcacc gttggcgccg ctgcgctgga
     1561 tttcctcgag cacggcgcag gcggcggcca ggtccatgcc ggcgccgccg tactcctccg
     1621 ggatcagcac cgaaagataa ccggcttcgg tcagcgcctg gacgaactct tccgggtaag
     1681 ccttgtcgcg atccttgtca cgccagtact catcgggata atcgacgcac aatcgtgcaa
     1741 cggcatctcg gacttctgga taggatcgat cttcggacat aggtcagcca ggcaaaaaaa
     1801 cgagtttacc cgatttacag cgaaaaacgg taggtcacgc tcaaccagac aatggcctat
     1861 gccgaaataa tattatgctc gggcccagag gggaaaccgg taatgttttc agccccctgt
     1921 tcaccaatga ccaggatatc gtgctcgcgg taaccgcctg cgcccggtaa gccctcgggg
     1981 atcgtcagca taggctccat cgacaccacc atgcctggct cgagcacggt atcgatgtct
     2041 tcgcgcaact cgagccccgc ctcgcgtcca tagtaatgcg acagcacgcc gaaggaatgg
     2101 ccatagccga aggtccggta ttgcaacaga tcgagatcgg caaagtagcg gttgatctcg
     2161 gcgcaaatgc cggaacaggt cgcgcccggc ttgatcagag acaagcccag ttcgtgcgcg
     2221 ccgacattag cctgccatat tctcagggaa tcggtatccg gttcaccgat aaacagcgta
     2281 cgttcgagcg cggtgtaata gcccgatatc atcgggaagg tattcagact caggatatcg
     2341 cccgcctgca acgcacgcgt cgtaaccggg ttgtgcgcac cgtcggtatt gatccccgat
     2401 tgaaaccaga cccaggaatc gcgtatctcg ctatcaggat aggcgcgcgc tatttcgagc
     2461 tccatcgcgt cgcgaccggc catcgcgaca tcgatttcgc gagcgcccac ccgtattgca
     2521 tcgcgaatgg cctcgccacc gatatcggcg gtacgcgccc cgcccttgat cagctcgatt
     2581 tcctcgaccg actttatcat gcgcagaccc atgatttccc gattcatatc gcaggcctcg
     2641 gcgtccccga gaaactcgga cctcagttct cgcgcggcga gggtcatatg atcaccttcg
     2701 atgccgacgc gacgcgcctg cgggatgatc gagcgcaccg cgcgccagta attgttgcgc
     2761 tgccagtcgg tataaatcag gttgtcgccg aaaccgcgcc gccagggctg gccgagatcg
     2821 atattcgccg agacggtaac gcagcgctcg gcggtcacga cacaggcgta aggccggcca
     2881 aaggagcagt agagaaagcc ggagtaatag gcgatgttgt gcatcgaggt cagcagcatc
     2941 gcgtcgatgc cgctactcgc cattaactcg cgcagcgcgc ccagccgggt ttcatactcg
     3001 gcgtcggaaa aaggtaactt cgctttcgaa ccgttatgca gttcgaagaa ttcgggacgt
     3061 tggacttgag acatgattat cactccgaag tattccgggt gacggtttca cccggcaaac
     3121 aatccgggcg tacaggatca agatatctgc ttaacataca gcgacgccat cgctgccagc
     3181 aaggggccga aattttatcg atgacgccac cggcaggcaa gttataatcg agcgttaccg
     3241 ggtcgcctgg ggaacgccat catatgaaat cgacaaccta tcaaatcaga cccgcgaccg
     3301 cggccgaccg cgcgcaatgg gatcccctgt ggcagggcta cctcggattc tacgagacga
     3361 ccctggcgcc ggaggtcacc gatctgctgt ggcaacgcat cctcgatccg gcacacgaaa
     3421 tcgagtgccg cttggcggca gcggcagacg ccagcctggc aggcctggtc cattactttc
     3481 cgcatgcaca tacctggtat gccaatccgg tttgttacct gaacgatctg ttcgtgttac
     3541 cgggtatccg cggcggaggt atcggcaagc tgctgatcga agccgtggct gacgaggcga
     3601 aacaaagagg ctggagcgag gtttactggc atacccagca gcacaacaca gtcgcacggg
     3661 gtttgtacga caagctgacc ggcggcaccg acggcttcgt caactacacc atcgaagtaa
     3721 ccgctcggga aacctgagct tcgatgaaag cagggcgtta atgaaccagg cgggaaaact
     3781 gttttttagc cctggtcatg cgacggctaa ataattaatc gattatcgct attgaccgcg
     3841 cgtcgtcgaa tgtcgtttat gtgctctggc aagtcgttta tcggttgact gtcgcgaccg
     3901 cattctggtt aaacttggcg ctacgaagtg ataatcgaaa tccgtcagca ctggcgacgg
     3961 tcgagcgccc cgaacgctc
//
LOCUS       DATCZV010000494         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_358438, whole genome shotgun sequence.
ACCESSION   DATCZV010000494 DATCZV010000000
VERSION     DATCZV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358438"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VIW27_11115"
     CDS             complement(<1..520)
                     /locus_tag="VIW27_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5740268.1"
                     /translation="MKFEGIYTPVVTPYRDDYSIDYPRLAEIIEFLIDRGVHGIISAG
                     TTGEYYAQSMEERFELMKFIGKQVDGRAAFIVGTGALRTEESIDYARGAVDVGADALL
                     VATPPYSIPTERENALHALAIDRVANLPILLYNYPGRMGANMGEEYLDRVGRSPNFCA
                     IKESSGDPNRVHL"
     gene            complement(534..1226)
                     /locus_tag="VIW27_11120"
     CDS             complement(534..1226)
                     /locus_tag="VIW27_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5740269.1"
                     /translation="MDTNLDKQAIYEDLKKQILTLRLEPGTALDETRLCERYQISRTP
                     LREILRGLAAEGCVDIVNNRGAYVSSMSHKMMRDFFVTAPMIYSAIGRLAAQNARPAQ
                     IAEIREIQQKFRAAIDSGVGEDMAYWNQCFHALMGEMADNPYLKPSYERLLIDHARIS
                     QTFYRPRDPRLAERMYEALEHHQRMIDAIEAGDSQAVVDLIVEHWELSRDLLEYFVKP
                     DPLAFEMAAANS"
     gene            complement(1388..2071)
                     /locus_tag="VIW27_11125"
     CDS             complement(1388..2071)
                     /locus_tag="VIW27_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEY5740270.1"
                     /translation="MIYVIVIVAVLTLIYGPQLWVQWVLNRYNRQPEANFPGTGGELA
                     RHLLDRYDLGEIRVEISDLGDHYDPLQRAVRLTRDKYEGKTLTAITVAAHECGHAFQH
                     AAAEPMFIWRTRLARISVYAQRLGSFMLFAAPFSVLVTKLPSAAVFNIAGAFLIMGFA
                     VVMHLLTLPVEVDASFKKALPLLGSGYLDSKQMPAARSILRAAAMTYVAASLASLLNF
                     WRWLAVLRR"
     gene            complement(2120..>2318)
                     /locus_tag="VIW27_11130"
     CDS             complement(2120..>2318)
                     /locus_tag="VIW27_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY5740271.1"
                     /translation="QELHRWAHTIKSSAASIGAMRLAHLAAQLEESFRKRVQIDLGRQ
                     LQFMQQEYRRVNASLENITDG"
BASE COUNT          448 a          694 c          722 g          454 t
ORIGIN      
        1 gcaggtggac gcggttcgga tcgcccgagc tttccttgat cgcgcagaaa ttcggcgagc
       61 ggccgacgcg gtcgaggtat tcctcgccca tattggcgcc catgcggccc ggatagttgt
      121 acaacaggat cggcaggttg gcgacacggt cgatggccag cgcatgcagt gcgttctcgc
      181 gctcggtcgg aatcgagtac ggcggcgtcg cgaccagcag cgcatcggcg ccgacgtcga
      241 ccgcgccgcg cgcgtagtcg atcgattcct cggtacgcag cgcgccggta ccgacgatga
      301 aggccgcgcg gccgtcgacc tgcttgccga tgaacttcat cagttcgaag cgttcttcca
      361 tgctctgtgc gtaatattcg ccggtggtac ccgccgaaat gatgccgtgc acacccctgt
      421 cgataagaaa ctcgatgatt tcggcaagcc gcgggtaatc gatgctgtaa tcgtcccggt
      481 aaggcgtgac caccggggta tagatacctt caaatttcat gcctgtttcc gcttcaagag
      541 ttagccgccg ccatttcaaa ggccagcggg tcgggtttga cgaaatattc gagcagatcg
      601 cgggacagct cccagtgttc gacgatcagg tcgaccacgg cctggctgtc accggcctcg
      661 atcgcatcga tcatgcgttg gtgatgttcg agcgcctcgt acatgcgctc ggcgagccgc
      721 ggatcgcgcg gccggtaaaa ggtctggctg atgcgcgcgt ggtcgatcaa caggcgctcg
      781 taactcggct tcaggtaagg gttgtccgcc atctctccca tcagcgcgtg aaagcactgg
      841 ttccagtaag ccatgtcctc gccgacgccg ctgtcgatcg cggcgcggaa tttttgctgg
      901 atttcgcgaa tttcggcaat ctgcgcgggc cgtgcattct gcgccgcgag gcgcccgatg
      961 gccgagtaaa tcatcggcgc ggtgacgaag aagtcgcgca tcatcttgtg gctcatcgac
     1021 gagacgtagg caccgcggtt attgacgata tcgacgcaac cctcggccgc caggccgcgc
     1081 agaatttcgc gcagcggcgt acgtgaaatc tggtagcgtt cacacagccg cgtctcgtct
     1141 agcgcggtac ccggctcgag cctcagggtc aagatctgct ttttcagatc ttcgtatatg
     1201 gcctgcttgt ccaggttcgt gtccagtcga aatttgtata cttattgtat acaaatttaa
     1261 tatctcgatg caatccagtc tcgatatttt tcacgcgccg atcgattgtc gcgcggcgcg
     1321 aggcctcggc aacaacggcg gagatcacgg taaagcgggc ctgccccgca agcccggcga
     1381 tacgatacta gcggcgcagc acggcgagcc agcgccagaa attgagtagt gaggccagcg
     1441 aggcggctac gtaggtcatc gccgcggccc gcaggatgga gcgtgccgcg ggcatctgct
     1501 tgctgtcgag gtacccgctg ccgagcagcg gcaaggcttt tttgaagctg gcgtcgacct
     1561 cgaccggcag cgtcaacagg tgcatgacca cggcaaaacc catgatcagg aaggcgccgg
     1621 cgatgttgaa caccgcggcc gaaggcagct tggtgaccag cacgctgaac ggcgccgcga
     1681 acagcatgaa cgaaccgaga cgctgcgcgt acaccgaaat ccgcgccaac cgcgtgcgcc
     1741 agatgaacat cggctcggcg gccgcgtgct gaaaggcatg gccgcactcg tgcgcggcca
     1801 ccgtgatcgc ggtcagcgtt ttaccctcgt acttatcgcg cgtcaggcgc acggcgcgct
     1861 gtaacggatc ataatgatcg cccaggtcgc tgatctcgac acggatttcg ccgagatcgt
     1921 agcgatcgag caggtgacgg gcgagttcgc cgccggtgcc ggggaaattg gcctcgggct
     1981 gccggttata ccggttcagc acccattgca cccacaactg gggaccgtaa ataagggtca
     2041 gcacggcgac gatgacgatg acgtagatca tgaccggcta gactaatccc tgcacggcca
     2101 ggttttcaag cgcggcgcgt cagccgtcgg taatattttc gagactggcg ttgacccgcc
     2161 gatattcctg ttgcatgaat tgcagctggc ggccgagatc gatctgcacc cgcttgcgaa
     2221 aagattcctc gagttgcgcg gccagatgcg ccaggcgcat cgcgccgatg ctggcggccg
     2281 acgacttgat ggtatgcgcc cagcgatgca attcctga
//
LOCUS       DATCZV010000495         5566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_358492, whole genome shotgun sequence.
ACCESSION   DATCZV010000495 DATCZV010000000
VERSION     DATCZV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358492"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="VIW27_11135"
     CDS             <1..2154
                     /locus_tag="VIW27_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY5740272.1"
                     /translation="GRIEVEIDSGAAQYLLDSGETLNLAYREVLLDLAPGPDGIGVDA
                     RLKLENGDDLYLAGQLPGTYLLDLDNGFRDLQATVRGRLSGLANVDALIPQIDKLAGR
                     LEFDLEIGGNLDEPRISGIARLSDVRLDLPDVDLSLTGLNLVAEADGGRGIDYRGTAE
                     TLGGEVEAAGDIRLDPAGGWPGKVTLQGKNFIPRGFLALWLPEEIDVDGRFDGEANLQ
                     FGAAIGLRGKLEIVSARGSILYPLLEGELDRLDYHDASARVIADEGGISGQSRVSTDE
                     GISFAGRFALPGARLLELDPENQLLEGSARLEVQQLQQLQLLSFDIERPEGSYELDVE
                     ASGTLARPQLAAHARLSDGEVGIPRLGIRVTGISMTGSTGDNNIFDFAIDARSGEGNL
                     RIEGSSVLDAGRGWPTRISIKGENFEAARIPEATVVMAPDLILQLEHRSIDISGDLYI
                     PFARIEPRDLTTAEQVSNDTVIVGDEQTEEPKWRITTRINLILGERVTLYGFGFEGRL
                     SGRLLVEEELGKPTVGTGEISIPEGRYRAYGQRLDIENGRVLFTGGPVANPGLDIRAT
                     RTSGSVVSGLQIGGRLQQPRIELFSIPAMGQTDILSYLLFGRPLETASGEDSALMAQA
                     ALAIGLAGGDRIARSLRDRFGLDDFRVEADDTGDQASLIIGRYLSSDVYVSYGVGLIE
                     SINSLNLRYRISDRWQLEAETGTYHGADILYTIER"
     gene            complement(2324..3091)
                     /locus_tag="VIW27_11140"
     CDS             complement(2324..3091)
                     /locus_tag="VIW27_11140"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5740273.1"
                     /translation="MGYDGVMVGYAWPSTPSRWAYIRDTDTSNGYARHFRKFLEFLAE
                     KTDAEEIHIIAYSNGTRLAARTFEQLAPMNDAKSRAEIHARHRLGNLVLVGSDLDREV
                     FASYLADGALDVARHTSIYVSDKDKALGFARWLTRQERLGQVIDEDKFSPAAKRVLAD
                     NREHISMIDVSDAEGAATGNGHGYFRNSPWASGDILMMLMHDLTPEQRGLRLKSSMPV
                     YDFPPDYMNRLWDALAGVNPEFRRAYMTRKSQQSVGQ"
     gene            complement(3098..3670)
                     /locus_tag="VIW27_11145"
     CDS             complement(3098..3670)
                     /locus_tag="VIW27_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740274.1"
                     /translation="MNTRNTLLLAAVVSMLAACSSVVWNEIDLMPPPDVYGDGMLDPL
                     PEQDPMDLIPYRGLLYATDRRPAAADDTEKYYANDRGQLVRLGVARISLHDADVNWSE
                     VREISLLKNRSNRYPLRVTGVREYGILEDTIPGFARREDYGEDLLDGDEEFAAAVNAK
                     LALSKRKHVYIYVHGYKVVFENPMLVALEL"
     gene            complement(3934..4677)
                     /locus_tag="VIW27_11150"
     CDS             complement(3934..4677)
                     /locus_tag="VIW27_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740275.1"
                     /translation="MNRILSLASLALLLTISIAGAVTDEAAKTAPAPQYLRVSVDRVV
                     LDTRGLAQASTSLAESIDELSLAIARLSSGNANLSDEDRATIASAVQSVDAAAAALRG
                     LAEEIPRTARDINDRLPRMISDAGQPIAALSSSLQAASDSVLLISESLPQATENAKAL
                     VDATLDAAVLRLSIYTFVLFAALALAVIAVIWFIYAQYLGPLARKLDEISGAPEQFAA
                     IAGHMQATSNNLLQLERLAAQRRLPKDDG"
     gene            complement(4735..>5566)
                     /locus_tag="VIW27_11155"
     CDS             complement(4735..>5566)
                     /locus_tag="VIW27_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5740276.1"
                     /translation="PLLFPRIQGADACGRIVAVGGGVDSRRIGERVLIRALQPRPTEV
                     NPVACWTFGSECDGAFAQYTVTGSGEAFGIESDLSDAELASFPCAYSTAENMLQRAGV
                     VKGERVFVTGASGGVGSAAVQLARRRGAHIVASCSAAKFEQVGALGAEQLVDRDRDPL
                     EALGKDSIDVVVDLVAGGGWPALPNILRAGGRYVSAGAIAGPIVEFDVRTLYLKDLTF
                     YGCTYQDTQVFDNLVGYIERGEIRPLLSKVYPLAEIAAAQQDFLDKKFTGKLVLEIPA
                     "
BASE COUNT         1065 a         1745 c         1730 g         1026 t
ORIGIN      
        1 ggcaggatcg aggtcgagat agattccggc gccgcgcagt atctgctcga ttccggggaa
       61 accctgaacc tggcctatcg ggaagttctg ctggacctgg cgcccggccc cgacggcatc
      121 ggcgtcgacg cccggcttaa gctcgaaaac ggtgacgatc tttaccttgc aggacagctt
      181 ccgggcactt acctgctcga cctcgacaac ggctttcgcg atttgcaggc gacagtccgc
      241 ggccggctct ccgggctcgc caacgtcgac gcgctgattc cgcaaatcga taagcttgcc
      301 ggccggctcg aattcgacct cgaaatcggc ggcaatctcg atgagccgcg gatttcgggc
      361 atagcccgcc tcagcgacgt gcgcctggat ctaccggacg tagacctgtc gctcaccgga
      421 ttaaacctcg tcgcagaggc cgacggcggc cggggcatcg actatcgcgg cacggcagaa
      481 accctgggcg gcgaggtcga agccgcgggt gatatccggc tggacccggc tggcggctgg
      541 ccgggcaaag tcactctgca gggcaaaaac ttcatcccgc gcggcttcct ggccttgtgg
      601 ttgcccgagg aaatcgatgt cgacgggcgc ttcgatggcg aggcgaatct acagttcggc
      661 gccgcgatcg gcctgcgggg aaaactggaa atcgtctcgg cccggggcag cattctctac
      721 cccctgctcg agggcgaact ggacaggctg gattaccacg atgccagcgc ccgggtgatt
      781 gccgacgaag gcgggataag cggccagagc cgggtctcca ccgacgaagg catttcgttt
      841 gccgggcgct tcgcattacc cggtgccagg ttgctcgaac tggaccctga aaatcagctc
      901 ctcgagggca gcgcgcgcct cgaggtccag cagttgcagc agttgcagtt gctgtctttc
      961 gatatcgagc ggccggaagg cagctacgaa ctcgacgtcg aggcttccgg cacgctggcc
     1021 aggcctcagt tggctgcgca tgccaggctg tcggacggag aagtcggcat cccgaggctc
     1081 ggtatacgcg ttaccgggat atcgatgacc gggagcacgg gagacaacaa tattttcgat
     1141 ttcgcgatcg atgcccgttc gggcgaaggt aacctgcgca tcgaaggcag tagcgtgctg
     1201 gatgccggca gaggctggcc gacccggatc agcatcaaag gcgagaattt cgaagccgcc
     1261 cgtatcccgg aagcgacggt ggtcatggcc ccggacctga tcctgcaact ggaacaccgt
     1321 tcgatcgaca tcagcggcga cctgtacata cccttcgcca ggatcgagcc ccgtgatctg
     1381 acaaccgcgg agcaggtttc caatgacacc gtgattgtcg gcgacgagca gaccgaggag
     1441 cctaaatggc ggattaccac gcgtatcaac ctgatcctgg gcgaacgggt aaccctgtac
     1501 ggcttcggtt tcgagggtcg gctcagcggc cgactgctgg tcgaggaaga actcggaaaa
     1561 ccgacagtcg gcaccggcga aatctcgatt ccggaagggc gctatcgcgc ctatgggcaa
     1621 cgcctcgata tcgaaaacgg gcgcgttctg tttaccggcg gcccggtggc caatcccggc
     1681 ctcgacatcc gcgccacgcg taccagcggc agcgtggtca gcggcctgca gatcggcggc
     1741 aggctgcagc aaccgcggat cgaactgttt tcgattccgg ccatgggaca gaccgatatc
     1801 ctgtcttact tgctgttcgg ccggccactg gagaccgcat ccggagaaga cagcgcgctg
     1861 atggcacagg cggcgctggc gatcggcctc gccggaggag atcgcatcgc gcgctccctg
     1921 cgcgatcgct tcggcctcga tgatttccgc gtcgaggccg acgataccgg cgaccaggcg
     1981 tcgctgataa tcggccgcta cctgtcttcg gacgtttacg tcagctatgg ggtggggctg
     2041 atcgaatcga tcaactcgct caacctgcgt taccgtatat ccgatcgctg gcagctggaa
     2101 gccgaaaccg gcacttacca cggcgccgat atactctaca cgatcgaacg ctgattgccg
     2161 ttggtcgctt ctatcggccg gcgagaactt gctagcgcac gatgtttact actcgagctg
     2221 caccgggatc gcgtatctgc cttatcgaat ggcgacctgt gcccccacct tcaggtcggt
     2281 tgcagcccgg agcaatctgg ctcgtcgagc ctccccatgg cgatcactgg ccgacgctct
     2341 gttgactttt gcgggtcata taggcgcggc gaaattcggg gttaaccccg gcaagcgcat
     2401 cccacagcct gttcatgtag tcgggcggga aatcatacac gggcatggat gatttcagtc
     2461 tgagaccgcg ctgttcgggg gtaaggtcgt gcatcagcat catcaggata tcgccgctgg
     2521 cccaggggct attgcgaaaa tacccgtgcc cgttaccggt agccgcaccc tcggcatccg
     2581 acacgtcgat catgctgata tgctccctgt tgtcggccag tacgcgtttc gctgccggcg
     2641 agaatttgtc ttcatcgata acctggccca ggcgctcctg acgcgtcagc cagcgcgcga
     2701 aaccaagcgc cttgtccttg tccgacacat agatactcgt atggcgcgct acatcgaggg
     2761 cgccgtccgc cagataagac gcaaatacct cgcgatcgag atcgctgccg accagcacca
     2821 ggttgccgag cctgtgccgc gcatggatct ccgcgcgcga cttggcgtcg ttcatcgggg
     2881 cgagctgctc gaatgtccgt gccgccagcc gcgtaccgtt gctataggcg atgatgtgaa
     2941 tttcctcggc atcggttttc tcggcaagga actcgagaaa cttgcgaaaa tggcgggcat
     3001 atccattcga ggtatcggta tccctgatgt aagcccatct gctcggtgtc gagggccagg
     3061 cataaccgac catgacaccg tcgtagccca ggaaatgtca aagctccaag gccaccagca
     3121 ttgggttttc gaacaccacc ttgtagccgt gcacatagat atatacgtgt ttgcgcttcg
     3181 acagagcgag ctttgcattg accgctgcgg caaactcttc atcgccgtcg agcaggtctt
     3241 cgccatagtc ttcgcgcctc gcaaagccgg gaatcgtatc ctcgaggatg ccgtactcgc
     3301 ggaccccggt gaccctcagc ggatacctgt tactgcgatt cttcagcaga gatatttccc
     3361 tgacctcgga ccagttcacg tcggcatcat gcagggagat gcgcgcgacg cccaggcgta
     3421 ccagctgacc gcgatcgttg gcgtaatatt tttccgtgtc atccgcagcg gccggccgcc
     3481 gatcggtcgc gtaaagcaaa ccccggtacg ggatcagatc catcggatcc tgctcgggca
     3541 gcgggtccag catgccatct ccatagacgt ccggaggcgg catcaggtcg atttcattcc
     3601 agacaacact ggaacaggcc gccagcatcg aaactaccgc agcgagcaac agggtattac
     3661 gcgtattcat cgaggtgtaa ccaggatgat tcgaagatga tagagattag cattaatcgc
     3721 gccatgcttt caggcaaagg gatttggctg tttactcgac ccggggaagc gttgatctca
     3781 ctggcggaca cgccgaacta ccaggtcacg tcccgctgcg ttcgccatct gtatacggat
     3841 cgatggccgc tggagacgga aagcggcctg caccgggtat cgacccgtcg ggtatgatcg
     3901 ggcggcgagc gccgccacgg cagtggcgaa tcttcagccg tcgtctttcg gtagccgccg
     3961 ctgcgctgcc aggcgctcca gttgcagcag gttgttcgag gttgcctgca tatggccggc
     4021 gatcgccgcg aactgctccg gcgcgccgct gatttcgtcg agcttgcgcg ccagcggtcc
     4081 gaggtactgg gcgtatatga accagatcac cgcgatgacg gcgagcgcca gcgcggcaaa
     4141 cagcacgaag gtgtagatcg acagccgcag caccgcggca tccagcgtcg catcgaccag
     4201 cgcctttgca ttttctgtcg cctgcggcag ggattcgctg atcagcagca cgctgtcgct
     4261 ggccgcctgc aggctgctcg acagcgccgc gatcggctgt ccggcgtcgc tgatcatgcg
     4321 cggcaggcga tcgtttatgt cgcgcgcggt acgcggaatc tcttcggcca gtccgcgcaa
     4381 tgcggcggcc gccgcgtcga cgctttgcac cgcactggcg attgtcgccc tgtcttcgtc
     4441 cgacaggttg gcgttgccgg aggacaggcg cgcgatcgcc agcgacagct cgtcgatcga
     4501 ctccgccagc gacgtcgagg cttgagccaa accccgggtg tccagcacca cgcgatcgac
     4561 gctgacgcgc aggtactgcg gcgccggagc cgtcttcgcg gcctcgtcgg tgacggcgcc
     4621 ggcgatggaa atagtcagca gcaatgcgag agatgcgagc gaaagaatcc tgttcatggc
     4681 gaatacccgg gaaaagctaa cgtgcagttt aaccgaccgc ttcgacgacg gcgatcaggc
     4741 cggaatctcg agcaccagct tgccggtgaa tttcttgtcg aggaaatcct gttgcgcggc
     4801 ggcgatttcc gccagcggat acacctttga cagcagcggc cgaatttcgc cgcgttcgat
     4861 gtaacccacc aggttgtcga aaacctgcgt atcctggtaa gtgcaaccgt agaaagtcag
     4921 gtccttcagg tacagggtcc tgacgtcgaa ttcgacgatc ggcccggcaa tcgcgccggc
     4981 ggagacgtag cggccgcccg cgcgcaggat attcggcaac gccggccagc cgccgccggc
     5041 aaccaggtcg acgaccacgt cgatgctgtc cttgccgaga gcctcgagcg gatcacggtc
     5101 gcgatcgacg agctgttcgg cgccgagggc gcccacctgc tcgaacttcg ccgcactgca
     5161 gctggcgacg atatgcgcgc cgcggcggcg cgccagttgc accgcggccg agccgacgcc
     5221 gcccgaagcg ccggtgacga aaacccgctc gcctttcacg acgccggcgc gctgcagcat
     5281 gttttcggcg gtcgagtagg cgcagggaaa ggaagccagc tcggcgtcgc tcagatcgga
     5341 ttcgattccg aatgcctcgc cgctgccggt cacagtgtac tgcgcgaacg caccgtcgca
     5401 ttcggaaccg aaagtccagc aggcgacggg gttaacctcc gtcggccggg gttgcagcgc
     5461 gcgaatcaaa acgcgctcgc cgatccggcg cgaatcgaca ccgccaccga ccgcgacgat
     5521 acggccgcag gcgtcggctc cctgaatacg cggaaatagc agcggc
//
LOCUS       DATCZV010000496         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_782709, whole genome shotgun sequence.
ACCESSION   DATCZV010000496 DATCZV010000000
VERSION     DATCZV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782709"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VIW27_11160"
     CDS             <1..778
                     /locus_tag="VIW27_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740277.1"
                     /translation="LIIVGLIMVAVFAPWITGSDGLEPNLANRLQPPGAAHWFGTDDL
                     GRDIYDRIIWGSRITLYIVGLVTVIVVPIGLVIGIIAGYVGGLVDTVLMRLTDIFLAF
                     PRLILAMAFVAVLGPGLENAVLAIALTTWSPFARIARAEALTIRHSEFILAAQVQGVG
                     TAGILARHVMPLCLSSVIVRLTLDMAGIILTAAGLGFLGLGAQPPTPEWGAMISLGRQ
                     YLIDQWWVPTVPGIAILVVSLGFNLLGDGLRDVFDPKSGS"
     gene            782..1624
                     /locus_tag="VIW27_11165"
     CDS             782..1624
                     /locus_tag="VIW27_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740278.1"
                     /translation="MSAKPLLVVEDLKVHYQTPTGLVRAVDGVSFELGREKLGIVGES
                     GSGKSQTGRAILRLTAPGGRISARRLEFDGTDLLRADEKRLRQIRGGSISMIMQDPKF
                     SLNPVMSVGAQIEEAFRVHHDAGRRQARERTLAMLRAVRIREPERVYRAYPHQVSGGM
                     GQRIMIAMMMIPEPDIIIADEPTSALDVTVQLQVLAVLDDMLRERGMGLIFISHDLNL
                     VASFCDRILVMYAGRVVEEIAAAELHEARHPYTRGLLDCLPSLDGDQHELPVLVRDPG
                     WRVP"
     gene            1624..>2202
                     /locus_tag="VIW27_11170"
     CDS             1624..>2202
                     /locus_tag="VIW27_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5740279.1"
                     /translation="MSGPIIDIRALDVFFGHGRWTHHAVRGVNLGVERGQSFGLVGES
                     GSGKTTVLRAVAGLLGGVARLDGEMAVAGEAQSGQRRSREFRRRVQMVFQDPYGSLHP
                     RHTVDTILAEPARVHRLDRLEERVVHALDEVGLDASFRFRYPHQLSGGQRQRVSIARA
                     LITGPEVVLLDEPTSALDVSVQAEVLNLLMRLR"
BASE COUNT          320 a          729 c          756 g          397 t
ORIGIN      
        1 gctgatcatc gtcgggctaa taatggtcgc ggttttcgcg ccgtggatca caggctcgga
       61 cgggctcgag ccgaatctcg ccaaccgcct gcagccgccc ggcgccgcgc actggttcgg
      121 caccgacgat ctcggccgcg acatctacga ccgaatcatc tggggctcgc gcatcacgct
      181 ctatatcgtc ggcctggtga cggtgatcgt ggtgccgatc gggctggtga tcggcattat
      241 cgccggctat gtcggcggac tcgtcgacac cgtgctgatg cgcctgaccg atatcttcct
      301 cgcctttccg cggctgatcc tggcgatggc tttcgtcgcc gtgctcgggc caggcctcga
      361 aaacgcggtg ctggcgatcg cgttgaccac ctggtcgccg ttcgcgcgta tcgcgcgcgc
      421 cgaggcattg acgatacggc atagcgaatt catcctcgcc gcgcaggtgc agggcgtcgg
      481 cacggccggc atcctcgcgc ggcacgtgat gccgctgtgc ctgtcgtcgg tgatcgtgcg
      541 cctgacactg gatatggccg gcatcatcct gaccgccgcc gggctcggct ttctcgggct
      601 cggcgcgcag ccgccgacgc cggagtgggg tgcgatgatc tcgcttggcc gccagtacct
      661 gatcgaccag tggtgggtgc cgaccgtgcc cggcatcgcg attctcgtgg tcagcctcgg
      721 attcaacctg ctcggcgacg gcctgcgcga cgtcttcgac ccgaagagcg gtagctgacg
      781 cgtgtccgcg aagccgttgc tcgtcgtcga ggacctgaag gtgcattacc agacgccgac
      841 cgggctggtg cgcgccgtcg acggcgtcag tttcgaactc ggccgcgaaa agctcggcat
      901 cgtcggcgaa tcgggctcgg gcaagtcgca gaccgggcgc gcgatcctgc gcctgaccgc
      961 gcccggcggg cgaatcagcg cgcggcggct cgaattcgac ggcaccgacc tgttgcgcgc
     1021 cgacgaaaag cgcctgcgcc agattcgcgg cggctcgatc tcgatgatca tgcaggaccc
     1081 gaagttttcg ctgaacccgg tgatgagcgt cggcgcgcaa atcgaggaag catttcgcgt
     1141 gcaccacgat gccggccgcc ggcaggcccg cgagcgaacc ctggcgatgc tgcgcgcggt
     1201 gcgcattcgc gagccggagc gggtttaccg cgcctaccca caccaggtgt cggggggcat
     1261 gggacaacgc atcatgatcg cgatgatgat gatcccggaa ccggatatca tcatcgccga
     1321 cgagccgacc tcggcgctcg acgtgaccgt gcagttgcag gtgctggcgg tgctcgacga
     1381 tatgctgcgc gagcgcggca tgggcctgat cttcatcagc cacgacctca acctggtggc
     1441 ttcgttctgc gatcgaatcc tggtcatgta cgccggccgg gttgtcgagg agatcgccgc
     1501 ggcggagctt cacgaggcac gccaccccta tactcgtggc ttgctcgact gcctgccgag
     1561 cctcgacggc gaccagcatg agttgccggt gctggtgcgc gatcccggct ggcgagtgcc
     1621 gtgatgagtg ggccgatcat cgatattcgc gcgctcgacg tgttcttcgg tcacggtcgc
     1681 tggacgcacc acgcggtgcg cggcgttaac ctcggcgtcg agcgcggcca gtccttcggc
     1741 ctggtcggcg aatccggctc cggcaagacc acggtactgc gtgcggtcgc cggcctgctc
     1801 ggcggggtcg cgcggctcga cggcgaaatg gcggtggccg gcgaggcgca gtccgggcaa
     1861 cggcgttcgc gtgaatttcg acgccgcgtg caaatggtgt tccaggatcc ctacggttcg
     1921 ctgcacccgc ggcataccgt ggacacgatt ctcgccgagc cggcgcgggt gcaccggctc
     1981 gaccggctgg aggagcgcgt cgtgcatgcc ctcgacgagg tcgggctcga cgcgtcgttt
     2041 cgcttccgct acccgcacca gctttccggc ggacagcggc aacgggtgtc gatcgcgcgc
     2101 gcgctgatca ccggtcccga ggtcgtgctg ctcgacgagc cgacctcggc gctcgatgtc
     2161 tcggtacagg ccgaggtact caacctgctg atgcgtctca gg
//
LOCUS       DATCZV010000497         3134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_305331, whole genome shotgun sequence.
ACCESSION   DATCZV010000497 DATCZV010000000
VERSION     DATCZV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305331"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VIW27_11175"
     CDS             <1..723
                     /locus_tag="VIW27_11175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5740280.1"
                     /translation="NILTNQIEYSVGDATHQGYLAYDDESSAPRPGIVVVHEWWGVND
                     YIRRRAHMLAELGYTALAIDMFAGRTASNPDEAGGLMTGVLNDMETGTRALQAGYQLL
                     LDQPGVDHQRTAAIGYCFGGAMVLHMARIGMPLSAVASFHGALGSFHTAEPGSIKAKI
                     LVCHGGADAMVSMDDVEAFKQEMNAAQADYEVLVLDGAMHGFTSPEADENGARYGIPV
                     GYQQQADEKSWEALQALLKRVF"
     gene            723..1370
                     /gene="pdxH"
                     /locus_tag="VIW27_11180"
     CDS             723..1370
                     /gene="pdxH"
                     /locus_tag="VIW27_11180"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY5740281.1"
                     /translation="MSLEMDLTDVRRAYESQGISRKDMDSDPLVEFGHWLQRARDLEL
                     LDATAMTLATADRDGRPSARIVLLKHYDADGFCWYSDDRSRKGRELAENPRAALLFHW
                     RDLSRQVRVHGEVRKLSAEAAEKYFLMRPEGSRFSAAASRQSSEIDSRQQLEDEVLRL
                     RAAYPDGDVPRPEDWIGYLLVPDYFEFWQGQGDRLHDRIVYEPLDGGWRMKRISP"
     gene            1370..1822
                     /locus_tag="VIW27_11185"
     CDS             1370..1822
                     /locus_tag="VIW27_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY5740282.1"
                     /translation="MLAAALIAFGHFLAFFSLTAAVVLQLALVSESMSVETARRIQRA
                     DRFAGISAVLLLVFGFLRVLQFEKGSAYYFSNSFFVVKLALFFIAAGISLYPTISYLR
                     WNRDLAQGAAPAIGTASVKRIKSAIHWQLVLILGILLCASLMAKGFGS"
     gene            1855..2601
                     /locus_tag="VIW27_11190"
     CDS             1855..2601
                     /locus_tag="VIW27_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY5740283.1"
                     /translation="MSFCKRLRNDELLLGTMLTLPSPEIAEMVSRCGFDWLFMDGEHG
                     PLSILDWQRQIQAVGGRCASILRVPSASERDLKKALDIGADGIIAPMVNDAEQARRVV
                     GYCKYPPRGRRGVGLARAQGYGYAFAEYMASANDETAVIVQAEHIDAIENIDEIVQVD
                     GIDAVFIGPYDLSASMGKTGEVGADDVVAAIERVTVACQGRGIALGYFGLDAASVRLY
                     MERGYRLICAGTDAGFVTAGASETLAALRS"
     gene            2766..>3134
                     /locus_tag="VIW27_11195"
     CDS             2766..>3134
                     /locus_tag="VIW27_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY5740284.1"
                     /translation="MSTNNKTMSLAEVEDLTRRALAGCGAHGAQLEIATQSVVDAECD
                     GIRTVGLGYLPLYCGHLQVGKINRDAAPTHAQIAPSVLRVQADSGFAHAAYAAAEADF
                     YALAKSQGIAAMSIVDSYSAG"
BASE COUNT          541 a          984 c         1058 g          551 t
ORIGIN      
        1 aatattctaa ccaaccagat cgagtacagc gtcggcgacg ccacgcatca gggctacctg
       61 gcctacgacg acgagagcag cgcgccgcgt ccgggaatcg tggtggtaca cgagtggtgg
      121 ggtgtcaacg attacattcg gcggcgcgcg cacatgctcg ccgaactcgg ttacactgcg
      181 ctcgccatcg acatgttcgc cggccgtacg gcgagtaacc ccgacgaagc cggcgggctg
      241 atgaccggcg tgctcaacga catggagacc ggcacgcgcg cgctgcaggc gggttaccag
      301 ctgttgctcg accagcccgg agtggatcac cagcgtaccg ccgcgatcgg ctattgcttc
      361 ggcggtgcga tggtgttgca catggcgcgc atcggtatgc cgctgtccgc ggtggccagc
      421 ttccacggcg cgctgggctc ttttcatacc gccgaacccg gcagcatcaa ggcgaagata
      481 ctcgtttgcc acggcggcgc cgacgcgatg gtttcgatgg acgatgtcga ggcattcaag
      541 caggaaatga atgccgcaca ggccgattac gaggtgctgg tactggacgg cgccatgcac
      601 ggcttcacca gtcccgaggc cgacgaaaac ggcgcccggt acggcatccc cgtcggctac
      661 cagcagcagg ccgacgagaa atcctgggag gcgctgcagg cattgctgaa gcgggtattc
      721 tgatgagcct ggagatggac ctcaccgatg tgcgtcgcgc ctacgaatcg cagggcattt
      781 cgcgcaagga catggattcc gatccactgg tggagttcgg ccactggctg cagcgcgccc
      841 gcgatctcga gttgctcgac gccaccgcga tgacgctggc gactgccgat cgcgatggcc
      901 gcccgtcggc gcgtatcgtg ctgctcaagc attacgatgc cgacggcttt tgctggtaca
      961 gcgacgaccg cagccgcaag ggccgcgaac tggccgagaa cccgcgcgcg gcgctgctgt
     1021 tccattggcg cgacctgagc cgccaggtgc gcgtgcacgg cgaggtgcgc aagctgtccg
     1081 ccgaggccgc cgagaaatac ttcctgatgc gccccgaggg cagccgcttc agtgccgcgg
     1141 catcgcggca gtcgagcgaa atcgacagcc gtcagcaact cgaggacgag gtgctgcggc
     1201 tgcgcgcggc ctacccggat ggcgacgtgc cgcggcccga ggactggatc ggttacctgc
     1261 tggtacccga ctacttcgag ttttggcagg ggcagggcga tcgcctgcac gatcgcatcg
     1321 tctacgaacc gctggacggc ggctggcgaa tgaaacgcat ctcgccctga tgctggcggc
     1381 ggcgctcatc gccttcgggc atttcctcgc attcttctcg ctcaccgcgg cggtggtgct
     1441 gcaactcgcg ctggtcagcg aatccatgag tgtcgaaacc gcgcgcagaa tccagcgcgc
     1501 cgaccgcttc gccgggattt ccgcggtgct gctgctggtg ttcggatttc tgcgcgtgtt
     1561 gcagttcgaa aagggcagcg cgtattattt ttccaacagc ttctttgtcg tcaagctggc
     1621 gctgtttttc atcgccgcgg gtatctcgct gtacccgacg atcagctacc tgcgctggaa
     1681 ccgcgatctc gcgcagggcg ctgcgccggc gatcggcacc gcgtcggtga agcgcatcaa
     1741 gtcggcgatt cactggcaac tggtgctgat tctgggcata ttgctgtgcg cgtcgctgat
     1801 ggcgaagggt ttcgggtcgt aaagcggccg caacggttca agggggtatt cgagatgagt
     1861 ttttgcaaac gcctgcgcaa cgacgagtta ttgctcggta ccatgctgac gctgccctcc
     1921 ccggagatcg cggagatggt ttcccgctgc ggcttcgatt ggctgttcat ggacggcgag
     1981 cacggcccgc tgtcgatcct cgactggcag cgccagatac aggcggtggg cggtcgctgc
     2041 gcgagcattc tgcgcgtacc gtcggccagc gagcgcgatc tcaagaaagc gctcgatatc
     2101 ggcgccgacg gcatcatcgc gccgatggtc aacgacgccg agcaggcgcg gcgcgtggtc
     2161 ggttattgca agtacccgcc gcgcggccgg cgcggtgtcg ggctggcgcg cgcccagggc
     2221 tatggttacg cgttcgccga gtacatggcc agcgccaacg acgagaccgc ggtgatcgtg
     2281 caggccgagc acatcgacgc gatcgaaaac atcgacgaaa tcgtgcaggt tgatggcatc
     2341 gatgcggtgt tcatcgggcc ctacgacctg tcggcgagca tgggcaagac cggcgaggtc
     2401 ggcgccgacg acgtggtcgc ggcgatcgag cgcgtgaccg tcgcctgtca gggtcgaggc
     2461 atcgcgctcg gttacttcgg cctcgacgcc gccagcgtgc ggctctacat ggagcgcggc
     2521 taccggctga tttgcgccgg caccgacgcc ggcttcgtca ccgccggcgc cagcgaaacc
     2581 ctcgcggcgc tgagatcgta acttttgccc gcggtaattt gcgcgccagc gcggcgcggc
     2641 ccggcgtcaa accgaatacc gtgccgttag cggaagatcc ccgcttgcgc gggggccact
     2701 ggcaacagca gacattcccg caacaaccgc tcgcggcgtg gcggtgaaat ttgaggagag
     2761 acgagatgag tacaaacaac aagacgatgt cgctggcgga ggttgaagac ctgacccggc
     2821 gtgcgctcgc cggttgcggc gcgcatggcg cacaactcga gatcgcgacg cagtcggtgg
     2881 tggatgccga gtgcgacggc attcgcaccg tcggcctcgg ctacctgccg ctgtactgcg
     2941 gccatttaca ggtcggaaag atcaaccgcg acgccgcgcc gacgcacgcg cagattgcgc
     3001 cgtcggtgct gcgcgtacag gccgacagcg ggttcgcgca cgcggcctat gctgcggccg
     3061 aagcggattt ttacgcgctc gcgaaaagcc agggcatcgc cgcgatgtcg atcgtcgatt
     3121 cctattcggc cggg
//
LOCUS       DATCZV010000498        16175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_782845, whole genome shotgun sequence.
ACCESSION   DATCZV010000498 DATCZV010000000
VERSION     DATCZV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782845"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VIW27_11200"
     CDS             complement(<1..219)
                     /locus_tag="VIW27_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5740285.1"
                     /translation="MSRQFSALERRLLNEFQQHLPLSSEPWAEIARQLGVYETTVLET
                     LQRLQTEGVISRVGAVFQPNRVGARTRAA"
     gene            complement(216..1382)
                     /locus_tag="VIW27_11205"
     CDS             complement(216..1382)
                     /locus_tag="VIW27_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEY5740286.1"
                     /translation="MKRMIAVLLSLLLFDTAAAARGSGDLGLLVERSSGSVQIVETSG
                     NSSIASVNGLGDLSHASVVYSRDQRYAYVFGRDGGLTRVDMLSAEISCRKLQAGNSIG
                     GAISQDGRLVAVSNYEPGGVRVFDAATLEPVADIAATWDQGRQRSKVVGLVDAPGNRF
                     VFSLWEAGEIWILDMTDPADPAITKFANVGRNPYDALITPDGRYYIAGLFGEDGLALL
                     DLWNPQLGVRRILPDYGRGTRKLPVYKMPHLEGWAVAGNLAFVPAVGSHEVLVIDMQS
                     WQPAGRIPVLGQPVFVMARPDGRQVWVNFALPDNHSVQVIDTQSLAVVRQLRPGKGVL
                     HMEFEPRGEQVWLSVRDENRVEVYDTDSFERVAVIPATRPSGIFFTARAHRIGL"
     gene            complement(1379..1699)
                     /locus_tag="VIW27_11210"
     CDS             complement(1379..1699)
                     /locus_tag="VIW27_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5740287.1"
                     /translation="MMRSTGFVLSLLLALPALSGDRISGARAGELTNLLIQDCGSCHG
                     LQMKGGLGPALLPRQLTGKSADYLSAVILEGRAGTAMPPWKALLSPAEARWMAERLLK
                     GLTP"
     gene            complement(1671..2477)
                     /gene="cobA"
                     /locus_tag="VIW27_11215"
     CDS             complement(1671..2477)
                     /gene="cobA"
                     /locus_tag="VIW27_11215"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY5740288.1"
                     /translation="MTTAGFVSLVGAGPGDPDLLTVKALRLLQSADVVVYDRLVSAEI
                     LQLIPHGVGRISVGKSPGMHSVPQSQIDAMLVGLAQSGRRVVRLKGGDPYIFGRGGEE
                     ALALVEHGIDFEVVPGITAAAGSSSYAGIPLTHRGLSHGVRFVTGQLQDGVNHKLDWR
                     CLADADSTLVIYMGLATLPQICAQLQRAGMPADTPAAAIHGATREHQRKVVNTLAGLA
                     EAVADAGLESPMTTIIGKVVGLSKQLDWFAAKREQDTEFDDDDAFDRIRA"
     gene            2725..4818
                     /locus_tag="VIW27_11220"
     CDS             2725..4818
                     /locus_tag="VIW27_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEY5740289.1"
                     /translation="MNTRLADRLSTRAAGVPRKLLIAGVLLLAGCVTAQPDISTPEQP
                     RAEETQPMIALEAWRDDVDFFIKYRLGDGLLYAGGNWHDRVELVERPAAENYAVPTLV
                     PIQYYREQPWDSLPRDAVEIPVFAVEQWRLLRDRLLRTVVPADGGGLVVDFGGFEYFL
                     YYDKGGEFQATRFNDKPANYRVRERLRFDQFMSRGRPILEHFLREQGITQTEFAFNTG
                     DTGWYSLPFLYINVERKLLVFVRNVPLTPGAKVRLPGLKGGQAFGHVLHSHLSNLLLR
                     PVSSLSRLFFTVTDTAVSTLRFDWTTGLAGKPVPPLADAEPMDLRQWEADLERIASRP
                     ATRGEVDLLVDGEAFFARFIDSVSRARESVRLQTYIFDNDDYATGIGELLKRRSNEGI
                     DVKVLLDGFGTITGTMAESESVPQHYRPPASVRRFLERDSRVEVRQKANPWFTGDHVK
                     STVVDQRVAFIGGMNIGREYRYDWHDLMIELRGPVVDEITHEFDLAWSHAGPLGDIGY
                     LVGQAFRKPRRNEQAAGVPLRLLLTGPGNYEIHQAQLQAARRARSYIYLQNAYFTDDS
                     LLRELVMARRRGVDVRVIVPLETDHGPITRSNVLAVNLMLEHGIRVYIYPGFSHVKAS
                     IFDGWACVGSANFDRLSLRVNRELNIAGSDPEFSAQLLERLFERDFRVAPELTEPIPE
                     RWIDHLVEIVGDYLY"
     gene            4834..5406
                     /locus_tag="VIW27_11225"
     CDS             4834..5406
                     /locus_tag="VIW27_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5740290.1"
                     /translation="MSKLDRDKWDRRYADSSYRRGNPVTLLQEWLPRLAAGKALDVAC
                     GAGRNAIAMAHAGFRVDAIDISPVGLELAQKEAASLGLDINWIEHDLEQPYDFASDYD
                     LIVVLWYVNLPLIARLGDCLKPGGYLLCEEHLVAGGELAGPENPRFRVAPGALETAAR
                     GLDVLLYEETAATNDDGEPMASARLVARKP"
     gene            5655..6383
                     /gene="mtgA"
                     /locus_tag="VIW27_11230"
     CDS             5655..6383
                     /gene="mtgA"
                     /locus_tag="VIW27_11230"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY5740291.1"
                     /translation="MAFRLLRKLGSLFSLAALERYWRWGWRLLLVALLIDIGYLYGIW
                     PDWELYRQGPLQQSSFIQAYRTEQARRDDWPRLRWNPTPLAQIPHVMQRAVVAAEDSR
                     FWQHGGIDWQALREAMQHNYEQRRLVYGASTISQQTVKNLFLTASRDPLRKWHEAWLT
                     LGMEQNLSKRRILEHYLNIAEFGRGVYGVDAAARFYYGIPASRLQGWQAIELAASLPS
                     PVANNPQTRTRAFLRRVNRIRRYF"
     gene            complement(6402..7424)
                     /locus_tag="VIW27_11235"
     CDS             complement(6402..7424)
                     /locus_tag="VIW27_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5740292.1"
                     /translation="MPTSAHYSALSSWVLMIAKAIDSYGQNSRQLFAEAGLDHYRLRD
                     PLARFPYPSVVRLWQLATTACGDPAFGLRVASFWHPTTLYALGYAWLASTNLDEAFAC
                     AARYTRMVNTAASGVIQVEKTSASYRVLLDASHIKLQQEPAEEAADAAVAMLVRMCRA
                     AYGEHFDPLRVSLRHADHDCAQLYREEFRCPVEFAAAGNAIWLEPDTVFEPLATANPE
                     LVRINDAIVTDYLARLDRADIVMRVRSRLIEHLPSGGADEARIADELNLSLRSLQRKL
                     KEQGISFSQLLEETRRELGRDYVRDSQHSINEIAYLLGFTEPGNFSRAFRRWYGKSPS
                     EYRGSA"
     gene            7541..8509
                     /gene="lpxC"
                     /locus_tag="VIW27_11240"
     CDS             7541..8509
                     /gene="lpxC"
                     /locus_tag="VIW27_11240"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY5740293.1"
                     /translation="MVYSQPDHRISQCTLAEHIDYVGVGLHSGRSLSMKLLPAPPNSG
                     ICFVRRDLAPERSLIRASWRNVVDTHLCTVLGNEHGATLSTVEHLLAALRGSGVDNAQ
                     IEISAGEVPILDGSCAPLMKLIDAAGIVSQRLPRQAIWIESPIEVRQGERMAMLAPGD
                     WPRISVDIEFEHAAIGTQCYSVELHDQIFASEIAPARTFGFTSELDELRSRGLALGGS
                     IRNAVVLDDDGVVNAEGLRFADEFVRHKVLDCLGDLALAEAPIFGHFYAYRPGHRLNV
                     ALVREMFANRGAWRRIGYDEALRRIELCEGQRLAAAKSRPPQLPPR"
     gene            8592..10868
                     /locus_tag="VIW27_11245"
     CDS             8592..10868
                     /locus_tag="VIW27_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5740294.1"
                     /translation="MTKSAKRLAIPGLIALLVLAAATAFVAHAAAPAADYAGSARCAE
                     CHAAQHAAWTTSDHGLAMLPADAGKVLGDFDDVRVEFHGIATRLFRDGERLRVTTAGA
                     DGEPATFDIAYSFGHYPLQQYLVDIGDGRLQALNIAWDSRARHAGGQRWFHLRDDEKL
                     DAQSPFFWTRHLQNANSRCIECHSTGFAKNYAADSDAYASAWTEPGVGCEACHGPASR
                     HLELATTNQLSAAESGFRRKPAQRLAWAFRDGQPIASPSGTRDAAWIDTCGGCHSRRA
                     SLTAPDPPAGYHQHYRLALLESELYFADGQIDDEVFVMGSFLQSKMHGQGVTCADCHD
                     PHSGKPLAAGNALCAQCHQPAVYDIAAHHRHAIGSAGAQCVACHMPERLYMQVDWRRD
                     HGFALPDPWLSQRLGVPNACSACHRERDDAWAAAAMEQWGLEADADRWPFANRGLGRQ
                     DMRVFREYAEDSTVLELAPIRQATLVASLAGFPTRGALEVAAGNLGAEDPLLRRAAVD
                     ALQPIPAGLRWQLLSPLIGDPVKAVRLAVANRLAELRPQLDGADAERLDDLLDEYRES
                     LAYQADMPGGQLAIGSLELSLGYPILAERAYQRALAIEADFVPALLNLADLYRSLDSE
                     GEARALLEHALQVAPDDANVRQAMALHLVRTDKHAAALEQLAVAVEQGNAGPGHFYTY
                     AVALDSAGRTGDAIAALEAASRRFPNTVEIYYLQVSYMDKSGRSQGIRRYLSLLDEVA
                     GNNPQIRAWMKKYGGGAP"
     gene            10935..11540
                     /locus_tag="VIW27_11250"
     CDS             10935..11540
                     /locus_tag="VIW27_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740295.1"
                     /translation="MNSCRNFKYAALVLAALLLGACATTRPVHEWRNDGFSGTLDSVL
                     IIAAIKRSTQRRVYEDLFAETLSTRGVTATPGYTLMTSELEISRDTVEAAIRGRDIDA
                     VLVTRLLGIDEFETYHPPPSRVYYRSYYSYYDHALTEVGPGYYRRYKVLTIETALYDS
                     ASGELVWSMQSEAVEPSTPRPVIEDQINLTIERLAAQGLVP"
     gene            11785..13383
                     /locus_tag="VIW27_11255"
     CDS             11785..13383
                     /locus_tag="VIW27_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5740296.1"
                     /translation="MKPTGNYLSVPRPGVWLLCITALLAFGQAQAQAQKPNVLVIWGD
                     DIGTWNISHNNRGMMGYMTPNIDRIAKEGVAFTDYYGQQSCTAGRAAFVGGTVPVRTG
                     MTKVGLPGAVEGWQESDITVAAVMKSLGYATGQFGKNHFGDRDEHLPTNHGFDEFFGN
                     LYHLNAEEEPENRDYPGDYVLPNGKTFLEQWGPRGVLKSMANGDGTQTIVNTGPLTKK
                     RMETVDEETLAAAKEFILKNHREGKPWFTWWNGTRMHFRTHVKEEHVGISGPGGDEYS
                     DGMVEHDMHVGELLDLLDELGIADNTVVYYSTDNGPHYNTWPDAGTTPFRGEKNSNWE
                     GAYRVPAFVRWPGKFPAGTTLNGIVAHEDWMPTFAAAAGKADLKQDLLDGYKAIGRTY
                     RTHLDGYNMIDYFSGKIDKSPRKEFWYFNDDAQVVAIRLGDWKTVFYENRGRQFGVWM
                     EPFTELRIPLLFNLRRDPFEKAQHNSNTYYDWFLDRPYIIVPMQAAAAKFGQTFIEYP
                     PSQKPGSFNISAIVEKLKQQLGSH"
     gene            13600..15231
                     /locus_tag="VIW27_11260"
     CDS             13600..15231
                     /locus_tag="VIW27_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017033877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5740297.1"
                     /translation="MPNSKPRLILQITVDALRADLPQRYSHLFGEGGFRYLMEDGVNF
                     TNAHYRHANTETIVGHVSLATGTVPAAHGMIGNVWFDRKQRRLVYNIEDADYRLLTSG
                     ADVDSTTEIDPTQKAAKVEGRSPAAILAPTFSDELALATAGKALIFGVSVKDRGAVSL
                     AGHAGKAFWFSKSGGEFVTSNYYYSKYPDWVNAWNARKPALAYAGGAWQLSHEPAAYR
                     YGDADDRDYETDFPGFGRTFPHAYGAADDKYFTTRLTLSPAGDELTLDFVKTLLDNEP
                     LGADEIPDYLAVSFSSTDYVGHLFGASSLESEDNIARLDRTLAQLIDYIDEKIGIENT
                     LIVLSADHGQPEAPGHLHELGIHDAHYFDTRGLDKKPAIAALKRRFGVGEELIEAFFQ
                     PYVYLNRELIADKGLDQTQVEAAVAAELVRFDGVAAAVSSSALRGANLPDTELNRAIL
                     SNFHAARSGDIYLVFEPNVFINDFDGLSVASTHGSPWRYDTHVPVIFAGGGLQPRMVS
                     RAVTPYDIAPTLSGLLGIRQPAATVGNPLPEVLGY"
     gene            complement(15281..15577)
                     /locus_tag="VIW27_11265"
     CDS             complement(15281..15577)
                     /locus_tag="VIW27_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY5740298.1"
                     /translation="MDPLTPCVYILTNRPRGVLYIGVTTNLPQRVWQHRNKLVDGFSS
                     RYNTDRLVWHQTHVDMYSALTRERQLKKWHRSWKIELIESSNPGWEDLYAEIAG"
     gene            complement(15845..>16175)
                     /locus_tag="VIW27_11270"
     CDS             complement(15845..>16175)
                     /locus_tag="VIW27_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HEY5740299.1"
                     /translation="YHGSIVGITIAFMGLTYHLLPQLGFRRVEGRAARWQPAIYGGGQ
                     LLHIAGLAWSGGYAVARKSTDAERGIEQIAGMGLMGFGGLVSIIGGLLFLVLVYRAMR
                     PGRHRDT"
BASE COUNT         2888 a         5270 c         5160 g         2857 t
ORIGIN      
        1 cgccgcccgc gtgcgggcgc cgacccggtt gggctggaac acggcgccga cgcggctgat
       61 cacgccctcg gtttgcaatc gctgcagggt ttcgagcacg gtggtttcgt agacgcccag
      121 ctgtcgcgcg atttcggccc acggctcgga gctcagcggc agatgctgct ggaactcgtt
      181 cagcaggcgc ctttctagtg ctgagaactg gcggctcaca ggccgatccg gtgtgcgcgg
      241 gcggtgaaga aaataccgct cggccgggtt gccggaatca ccgccacgcg ctcgaaggag
      301 tcggtgtcgt atacctcgac gcggttctcg tcgcgcaccg acagccagac ctgctcgccg
      361 cgcggttcga attccatgtg cagcacgccc ttgccgggtc gcagttgcct gaccaccgcc
      421 agcgactgcg tatcgatgac ctgaacgctg tgattgtcgg gcagcgcgaa gttgacccag
      481 acctggcgcc cgtcgggacg cgccatgacg aacaccggct ggccgagtac cggtatgcgg
      541 ccggccggtt gccagctttg catgtcgatc accagtacct cgtgactgcc gaccgcgggt
      601 acgaaggcga ggttgccggc caccgcccag ccctcgaggt gcggcatctt gtagaccggc
      661 agtttgcgcg tgccgcgacc gtagtcgggc aggatacggc gcacgccgag ctgcgggttc
      721 cacaggtcca gcaatgccag cccgtcctcg ccgaatagcc cggcgatata gtaacggccg
      781 tcgggggtga tcaatgcgtc atagggattg cggccgacgt tggcgaattt ggtaatcgcg
      841 gggtcggccg gatcggtcat gtccagtatc cagatttcgc cggcctccca cagcgaaaac
      901 acgaagcggt tgccgggcgc gtcgaccagg ccgacgactt tcgaccgttg ccggccctgg
      961 tcccaggttg cggcgatatc ggcgaccggc tccagcgttg cggcgtcgaa aacgcgcacg
     1021 ccgccgggct cgtaattgga taccgccacc agccgcccgt cctgcgagat cgcgccgccg
     1081 atgctgttgc ccgcctgcag cttgcggcaa ctgatttcgg cgcttagcat atcgacccgg
     1141 gtgaggccgc cgtcgcggcc gaagacgtag gcatagcgct ggtcgcgcga atagaccacc
     1201 gaggcgtgcg acaggtcgcc gaggccgttg acgctggcga tactgctgtt gccgctggtt
     1261 tcgacgattt gcacactgcc gctggagcgt tcgaccagca ggccgaggtc gccgctgccg
     1321 cgcgcggccg ccgcggtgtc gaacagcagc agcgacagca ggaccgcgat catccgtttc
     1381 atggtgtcag gcccttcagc agccgctcgg ccatccagcg cgcttcggcg ggcgagagga
     1441 gggctttcca cggcggcatc gcggtgccgg cgcggccttc gaggatcacc gcgctgaggt
     1501 agtcggcgga cttgccggtg agctggcgcg gcagcagcgc gggcccgaga ccgcctttca
     1561 tttgcaggcc gtggcaggaa ccgcagtcct ggatcagcag gttggtgagc tcgccggcgc
     1621 gcgcgccgct gatgcggtcg ccggacagcg ccggcagcgc cagcagcagg ctaagcacga
     1681 atccggtcga acgcatcatc gtcgtcgaac tccgtatcct gctcgcgctt cgccgcgaac
     1741 cagtcgagct gcttgctgag gcccacgacc ttgccgatta tcgtggtcat cggcgactcg
     1801 agcccggcgt cggcgactgc ctcggccagt ccggccagcg tattgaccac cttgcgctga
     1861 tgctcgcggg tagcgccatg aatcgccgct gccggcgtgt cggccggcat tcccgcgcgc
     1921 tgcagctgcg cgcagatttg cggcagggtc gcgagtccca tgtagatcac cagggtgctg
     1981 tcggcatcgg ccaggcagcg ccagtcgagt ttgtggttga caccgtcctg caattgcccg
     2041 gtgacgaaac gcacgccgtg gctgaggccg cggtgggtca gcggaatgcc ggcgtaactg
     2101 ctgctgccgg cggccgcggt gatgcccggc accacttcga agtcgatgcc gtgctcgacc
     2161 agtgccaggg cttcctcgcc gccgcggccg aagatatagg gatcgccgcc tttcagtctc
     2221 accacgcggc ggcccgactg cgccaggccg acgagcatcg catcgatctg tgattgcggc
     2281 acactgtgca tgccgggcga tttgccgacc gagatacgcc ccacgccgtg cggaatcagt
     2341 tgcaggatct cggcgctgac caggcggtcg tagacgacca cgtcggcgct ctgcagcagg
     2401 cgcagggcct tgaccgtcag cagatcggga tcgccgggcc cggcacccac cagtgatacg
     2461 aagcctgcgg tcgtcatttg tcggtcctgt tggcggcgtg gaccgggtgc cggattgcag
     2521 cgagttgcat gatctaaaac tcctgtttcc tggccattat tgatacccgt cgcgctgctg
     2581 tatttgataa atgtcaaccc tccccggcag cccagggaag gccgctgctg gcggtcgata
     2641 gacgctatcc agggaggcgg ccggatcggc gcttatcggc cgtgatcact tcccaactcg
     2701 atgccgcttt gccatgatgg accgatgaac accagattgg ccgatcgact gtcaacgcgg
     2761 gctgccgggg tgccgcgcaa gctgctcatc gctggcgtcc tgctgctggc cggctgcgtc
     2821 accgcgcaac ccgacatttc gactcccgag caaccccggg ccgaggagac gcagccgatg
     2881 atcgcgctcg aagcctggcg cgacgatgtc gattttttca tcaagtatcg cctcggcgac
     2941 ggcctgctat acgccggcgg caactggcac gaccgcgtag agctggtgga gcgaccggcg
     3001 gcggaaaact acgcggtgcc gacgctggtg ccgatccagt actaccgtga acaaccctgg
     3061 gattcgctgc cgcgggacgc cgtcgagatc ccggtgttcg cggtcgagca gtggcggctg
     3121 ctgcgcgatc gcctgttgcg cacggtggta cccgccgacg gtggcgggct ggtggtggat
     3181 ttcggcggct tcgagtattt cctgtattac gacaagggcg gtgagttcca ggcgacccgc
     3241 ttcaacgaca agccggccaa ttaccgcgtg cgcgagcgcc tgcgcttcga tcagttcatg
     3301 tcccgtgggc gtccgatcct cgagcatttc ctgcgcgagc agggcatcac gcagaccgag
     3361 ttcgcattca acaccggcga taccggctgg tattccctgc cgttcctgta tatcaacgtc
     3421 gagcgcaagc tgctggtatt cgtcagaaac gtgccgctga cgccgggtgc gaaggttcgg
     3481 cttccggggc tcaagggcgg ccaggctttc gggcacgtgc tgcacagtca tctcagcaac
     3541 ctgctgctgc gtcccgtgtc gtcgctcagc cggctgttct tcacggtcac cgataccgcg
     3601 gtgtcgacgc tgcgcttcga ctggacgacc ggcctggccg gcaagccggt tccgccgctg
     3661 gccgacgccg aacccatgga cctgcggcaa tgggaagccg atctcgagcg gatcgccagc
     3721 cgcccggcga cccgcggcga ggtcgatctg ctggtcgacg gcgaagcctt tttcgcgcgc
     3781 ttcatcgaca gcgtcagcag ggcccgcgaa tcggtgcgcc tgcagaccta catattcgac
     3841 aacgacgatt acgccaccgg catcggcgaa ttgctgaaac gccgatccaa cgagggcatc
     3901 gacgtcaagg tgctgctcga cggcttcggc accatcaccg gcaccatggc ggaatcggag
     3961 tcggtaccgc agcactaccg gccgccggcc tcggtgcggc ggtttctgga gcgcgattcg
     4021 cgagtcgagg tgcgccagaa ggccaatccc tggtttaccg gcgaccacgt caagtcgacc
     4081 gtcgtcgatc agcgggtcgc cttcatcggc ggcatgaata tcggccgaga gtatcgttac
     4141 gactggcatg acctgatgat cgagttgcgc gggccggtgg tcgacgagat cacgcacgag
     4201 ttcgatctcg cctggagcca cgcgggaccg ctcggcgata tcggttacct ggtcggacag
     4261 gcgttcagaa agccgcggcg caacgagcag gcggcaggcg tcccgctgcg cctgttgctg
     4321 accgggcccg gaaactacga aatccaccag gcccagttgc aggccgcgcg ccgcgcgcgc
     4381 agctacatct acctgcagaa tgcctacttc accgacgaca gtctgctgcg cgaactggtg
     4441 atggcccgcc gccgcggcgt cgacgtgcgc gtcatcgtgc cgctggagac cgatcacggc
     4501 ccgatcacgc gcagcaacgt gctggccgtc aacctgatgc tcgaacacgg cattcgagtg
     4561 tatatttatc ccggtttctc gcacgtcaag gcatcgatat tcgatggctg ggcctgtgtc
     4621 ggttcggcga acttcgaccg cctgagcctg cgggtgaacc gcgaactgaa tatcgccggt
     4681 tccgacccgg agttttcggc gcaactgctc gagcgcctgt tcgagcggga tttccgggtg
     4741 gcgcctgaat tgaccgaacc gatacccgag cgctggatcg atcacctggt cgagatcgtc
     4801 ggcgactacc tttactgaaa ccgcgaggtg acgatgagca aactggaccg tgacaagtgg
     4861 gatcggcgtt atgccgatag cagctatcgc aggggcaatc cggtaacgct gctgcaggag
     4921 tggctgccgc gcctggccgc cggcaaggct ctcgacgtcg cctgcggtgc ggggcgcaac
     4981 gcgatcgcca tggcgcacgc cggctttcgc gtcgatgcga tcgatatctc tcccgtcggc
     5041 ctcgagctgg ctcaaaaaga ggccgcaagc ctgggtctcg acatcaactg gatcgagcac
     5101 gacctggagc aaccgtacga cttcgctagc gactacgacc tgatcgtcgt gctctggtac
     5161 gtcaacctgc cgttgatagc gaggctcggc gattgcctga aacccggcgg ttacctgctc
     5221 tgcgaggagc acctggtggc tggcggcgag ttggcaggtc ccgagaaccc gcggtttcgg
     5281 gtcgcgccgg gtgcgctcga gacggccgca cgtgggctcg acgtgctgct ctacgaggaa
     5341 accgcagcga ccaacgacga cggcgaaccg atggccagcg cgcggctggt ggcccgtaag
     5401 ccctgattcg aattcactgt tgcgggcacc gcctgccgcc gctactcgtc accgccgcgc
     5461 taagcctcat gcccgcgtaa gcggcggtcc gccgcatcgg cagcttgcgg tggcgttgcc
     5521 gtagcggaga attcctgcgg gcctccaacg gctggtgccg aaatgactcc agctagcgaa
     5581 accggccccg gagagcggca agatcatcag ggacggcaac catccggtcg tcttatgtac
     5641 tagtatctgg gcctatggca tttcgtctgc tgcgaaaact cggatcgctt ttcagcctcg
     5701 ccgcgctcga gcggtactgg cgctggggct ggcgattgtt actggtcgcg ctgctgatcg
     5761 atatcggcta cctgtacggc atctggcccg actgggaact ctaccgccag gggcccctgc
     5821 aacaatcgag tttcatacag gcctaccgga ccgagcaggc gcggcgcgat gactggccgc
     5881 ggctgcgctg gaacccgacg ccgctggcgc aaatccccca cgtcatgcag cgcgcggtgg
     5941 tggccgccga ggattcgcgc ttctggcaac acggcggaat cgactggcag gccttgcgcg
     6001 aagccatgca gcataactac gagcaaagac gcctggtcta cggcgccagc acgatttcgc
     6061 agcaaaccgt caagaacctg tttctgaccg cgtcgcgcga cccgctgcgc aagtggcacg
     6121 aagcctggct gacgctcggc atggagcaaa acctgtcgaa gcggcgcatt ctcgaacact
     6181 acctcaatat cgccgagttc ggccgcggcg tttacggagt cgatgccgcg gcgcgctttt
     6241 attacggcat tccggcgtcg cgcctgcagg gctggcaggc aatcgagctg gccgcctccc
     6301 tgccgtcgcc ggtcgccaac aatccgcaga cgcgcacgcg cgcattcctg cggcgtgtca
     6361 acaggattcg gcgctacttt tgaactcgcc gccgggggtg gctaggcgct gccgcggtac
     6421 tcgctcggcg acttgccata ccagcgccga aacgcgcgcg aaaaattgcc cggctcggtg
     6481 aatcccagca ggtaggcgat ctcgttgatc gagtgctgcg aatcgcgcac gtaatcgcgc
     6541 cccagctcgc gccgcgtttc ctccagcagc tgcgaaaacg atattccctg ttccttgagc
     6601 ttgcgctgca ggctacgcag gctgaggttg agttcatcgg cgatgcgggc ttcgtcggcg
     6661 ccgccgctcg gcaagtgttc gatcagccgc gatcggaccc gcatgacgat atcggcgcgg
     6721 tcgagccgcg ccaggtaatc ggtgacaatg gcgtcgttga tacgcaccag ctcgggattg
     6781 gcggtcgcca gcggttcgaa aaccgtgtcc ggctcgagcc agatcgcgtt gccggcggcg
     6841 gcaaactcga ccgggcagcg aaattcctca cggtaaagct gcgcacagtc gtgatcagcg
     6901 tgccgcaggc tcaccctgag cggatcgaaa tgctctccgt aagccgcgcg acacatgcgt
     6961 accagcatcg ccaccgcggc atcggccgcc tcctccgccg gttcctgctg cagcttgatg
     7021 tgactggcgt cgagcaacac gcggtaggag gcggaggttt tctcgacctg gatgaccccg
     7081 ctggcggcgg tgttgaccat gcgtgtgtaa cgcgccgcgc aggcgaacgc ttcgtcgagg
     7141 ttggtgctcg ccagccaggc atagccgagc gcgtatagcg tggtcggatg ccagaagctc
     7201 gcgaccctga gaccgaacgc cggatcgcca caggcagtgg tcgccagctg ccacaggcgg
     7261 accaccgacg ggtacgggaa gcgcgccagc gggtcgcgca gtcggtagtg atcgaggccg
     7321 gcctcggcga atagctggcg gctgttctga ccgtaggaat cgatcgcctt ggcgatcatc
     7381 aaaacccacg acgagagggc cgaataatgt gcgctggttg gcattcctgc tgcccctgat
     7441 ttatcgggat aggcgattgg cgcccgcgga ttagcggcat tttagcaacc accatcacag
     7501 ttgcatacca agagatttca ataacttgag gtatgcaatc atggtttact ctcaacccga
     7561 tcatcgcatc tcgcagtgca cgctcgccga acacatcgac tatgtcggcg tcggcctgca
     7621 cagcggccgt tcgctgagca tgaagctgtt gccggcgccg cccaacagcg gtatctgctt
     7681 cgtacgccgg gaccttgcgc ccgagcgttc gctgattcgc gccagctggc gcaacgtcgt
     7741 cgatacacat ctgtgcaccg tgctcggcaa cgaacatggt gcaaccctga gtaccgtcga
     7801 gcacctgctg gcggcgttgc gcggcagcgg cgtcgacaat gcgcaaatcg aaatcagtgc
     7861 cggtgaggtg ccgatactcg acggcagctg cgcaccgctg atgaaactga tcgatgccgc
     7921 cggcatcgtt agccagcgtc tgccccgcca ggcgatctgg atcgaatcgc cgatcgaggt
     7981 tcgccagggc gagcgcatgg cgatgctggc gccgggtgac tggccgcgca tctcggtcga
     8041 tatcgagttc gagcatgccg cgatcggcac gcagtgttac tcggtggaat tgcacgacca
     8101 gattttcgcc agcgaaatcg cgccggcacg gaccttcggc ttcacctcgg aactcgacga
     8161 gctgcgcagc cgcgggctgg cgctgggcgg ctcgatacgc aacgccgtgg tgctcgacga
     8221 cgatggcgtc gtcaatgccg aggggttgcg cttcgccgac gagttcgtgc gccacaaggt
     8281 gctcgattgc ctcggcgacc tggcgctcgc ggaggcgcca atcttcggcc atttctatgc
     8341 ataccggccc gggcaccggc tcaacgtcgc actggtgcgc gaaatgttcg ccaaccgcgg
     8401 cgcctggcgg cggatcggct acgacgaagc cctgcggcgc atcgaacttt gcgagggaca
     8461 gcggctggcg gcggcgaaat cgcgcccgcc gcagcttccg ccgcgctgag cggcgagcgg
     8521 gaatttttca atcgcgcgaa acctgctagg ctggagcttt cgggccccgg cccgccagga
     8581 agattcacgc cttgacgaaa tccgccaagc ggctcgcgat cccgggcctg atagcgctgc
     8641 tggtgctcgc cgcagcaacc gcgttcgtgg cgcacgccgc cgcgccggcg gccgactacg
     8701 ccggctcggc gcgctgcgcc gaatgccacg ccgcgcagca cgcggcctgg accacttccg
     8761 accatggtct ggcgatgctg ccggccgacg ccggcaaggt gctcggcgat ttcgacgacg
     8821 ttcgcgtcga gtttcacggt atcgccacgc gcctgttccg tgacggcgag cgcttgcggg
     8881 tgaccaccgc cggggccgac ggcgagcctg cgaccttcga tatcgcctac agcttcggtc
     8941 attacccgct gcagcagtat ctggtcgata tcggtgacgg ccgcctgcag gcactcaaca
     9001 tcgcctggga cagccgcgcg cggcacgcgg gcggacagcg ctggtttcat ctgcgcgacg
     9061 acgagaagct cgatgcgcaa agcccgtttt tctggacccg gcacctgcaa aacgccaata
     9121 gccgctgcat cgagtgtcac agcacgggct tcgccaagaa ctacgctgcc gacagtgacg
     9181 cctacgcgag cgcctggacc gaacccggcg tcggctgcga ggcctgccac gggcccgcgt
     9241 cgcggcatct cgaactggcc acgacaaacc agctcagtgc cgccgaatcc gggtttcgac
     9301 gcaagcccgc acagcgcctt gcgtgggcgt ttcgcgacgg acaaccgatc gcctcgccga
     9361 gcggcacgcg cgacgctgcg tggatcgata cctgcggcgg ctgccactcg cggcgcgcgt
     9421 cgcttacagc tcccgatccg cccgccggct accaccagca ttaccgcttg gcgctgctcg
     9481 aatcggagct gtacttcgcc gacggtcaga tcgacgacga agtgttcgtg atgggttcgt
     9541 ttctgcagag caagatgcat ggccagggcg tgacctgtgc cgattgccac gacccgcact
     9601 ccggcaagcc gctcgccgcc ggtaatgcgc tgtgcgcgca atgccatcag ccggcggtct
     9661 acgatattgc cgcgcatcac cggcacgcga tcggctcggc cggcgcgcaa tgcgttgcct
     9721 gccacatgcc cgaacgtctc tacatgcagg tcgactggcg ccgtgatcac ggcttcgcgc
     9781 tgcccgatcc gtggctgtcg cagcgcctcg gcgtgcccaa tgcctgcagc gcgtgccacc
     9841 gtgagcgcga tgacgcctgg gccgccgcgg cgatggaaca atggggtctg gaagccgatg
     9901 ccgatcgctg gccgttcgcc aaccgcggtc tcggtcgcca ggacatgcgc gtgtttcgtg
     9961 aatacgccga ggattcgacg gtgctcgagc tcgcgccgat tcgccaggcg acgctggtcg
    10021 ccagcctggc cggttttccg acccgcggcg cactcgaggt cgccgccggt aatctcggcg
    10081 ccgaagaccc gttgctgcgc cgtgccgcgg tcgatgcgtt gcagccgatt ccggctggcc
    10141 tgcgttggca gttactgagc ccgttgatcg gcgacccggt gaaagcggtg cgccttgcgg
    10201 tcgccaaccg gctcgccgag ctgcgcccgc aactcgatgg tgccgacgcc gagcggctcg
    10261 acgatttgct cgacgagtac cgcgaatcgc tcgcctatca ggccgatatg ccgggcggac
    10321 agcttgctat cggcagtctc gagttgagcc tcggctaccc gatcctcgcc gagcgtgcct
    10381 atcagcgtgc gttggcgatc gaggcggact tcgtgccggc gctactcaac ctcgccgacc
    10441 tttaccgctc gctcgatagc gagggcgaag ccagggcgtt gctcgagcac gcgctgcagg
    10501 tcgcgcccga tgacgccaac gtgcgccagg caatggccct gcacctggtg cgcaccgaca
    10561 aacacgcggc cgcgctcgaa cagctggcgg tcgccgtcga acaaggcaat gcgggtccgg
    10621 ggcacttcta tacctatgcg gtggcgctcg actcggccgg caggaccgga gacgcgatcg
    10681 ccgcgctcga ggcggcaagc cgccgctttc cgaacactgt cgaaatctac tatctgcagg
    10741 tctcgtacat ggacaagagc ggccgaagcc aaggcattcg gcgctacctt tcgctgctcg
    10801 acgaggtcgc cggtaacaac ccgcagatac gcgcctggat gaaaaaatac ggcggcggcg
    10861 cgccgtgacc ggcagcattg ataattgcca gcgaatgcca taatctgaag tgcctgcgaa
    10921 aagggaccac gcgaatgaat tcctgtcgaa atttcaaata tgctgcgctg gtgctggccg
    10981 cgctgctgct cggcgcctgc gcgactactc gaccggtgca cgagtggcgc aacgacggtt
    11041 tctccggcac gctcgattca gtgctgataa tcgcggccat caagcgctcg acccagcggc
    11101 gcgtctacga agacctgttc gccgagaccc tgtcgacccg cggcgtgacc gcgaccccgg
    11161 gttataccct gatgacctcc gagctggaaa tctcgcgcga caccgtcgaa gcggcaattc
    11221 gcggccgcga catcgacgcg gtactggtta cgcggctgct cggcatcgac gagttcgaga
    11281 cttaccatcc gccgccctcg cgcgtctact accgcagtta ctacagctac tacgatcacg
    11341 cgctcaccga ggtcggtccc ggctactatc gacgctacaa ggtgctgacc atcgaaaccg
    11401 cgctctacga cagcgcctcg ggcgaactcg tctggtcgat gcagtccgag gccgtcgagc
    11461 cgtctacacc gcgtccggtg atcgaggacc agatcaatct gaccatcgaa cggctggccg
    11521 cccagggact ggttccgtga acgggtgatc cgcagcctgt ttgcgagcgt atcctagacc
    11581 gaggtgatgc tgcgggagag ggttccagtc cagcggatac ggtgtacaat ggcaccgcgt
    11641 gttgaggact accacccggc agcgatcaat aaattcagtt actcattcat cgatcggcgg
    11701 cgagcccgcg acgcgttccg tgaaggcggc agccgttatc caccgggaaa cccggtccag
    11761 gaaactacag ggagatataa tcaaatgaaa cccacaggta actacctgtc ggtgccgcgg
    11821 cccggagtct ggctgctgtg catcaccgcg ctgctggcat tcggccaggc gcaggcgcag
    11881 gcgcaaaaac ccaacgtgct ggtcatctgg ggcgacgaca tcggcacctg gaacatcagc
    11941 cacaacaacc gcggcatgat gggctatatg acgcccaaca tcgaccgcat cgccaaagag
    12001 ggcgtcgcgt tcaccgacta ctacggccag caatcctgca ccgccggacg cgccgcgttc
    12061 gtcggcggca cggtgccggt acgcaccggc atgaccaagg tcggcctgcc gggcgccgtc
    12121 gagggctggc aggaatccga tatcaccgtc gccgcggtga tgaaatcgct cggctacgcg
    12181 accggccagt tcggcaagaa tcactttggc gaccgcgacg agcatttgcc gaccaatcac
    12241 ggtttcgacg agttttttgg caatctctac catctgaacg ccgaagagga gcccgaaaat
    12301 cgggactatc cgggcgacta tgtattgccc aatggcaaga cttttctcga gcagtggggc
    12361 ccgcgcggcg tcctcaagtc gatggccaac ggcgatggca cgcagaccat cgtcaacacc
    12421 gggccgctga ccaagaaacg catggaaacc gtcgacgagg aaaccctcgc cgccgccaag
    12481 gaattcatcc tcaagaacca tcgcgagggc aagccgtggt tcacctggtg gaatggcacg
    12541 cgcatgcact tccgcaccca cgtcaaggag gagcatgtcg gtatttccgg gccgggcggc
    12601 gacgaataca gcgacggcat ggttgagcac gacatgcacg tcggcgaact gctcgacctg
    12661 ctcgacgaac tcggcatcgc cgacaacact gttgtctatt attccaccga caacggcccg
    12721 cactacaata cctggccgga tgccggcacc acgccattcc gcggcgaaaa gaactccaac
    12781 tgggaaggcg cctaccgcgt gccggccttc gtgcgctggc ccggcaagtt cccggccggc
    12841 accacgctga acggtatcgt cgcgcacgag gactggatgc cgaccttcgc cgccgccgcc
    12901 ggcaaggcag acctcaagca agacctgctg gatggctaca aggcgatagg tcgcacctac
    12961 aggacgcatc tcgacggcta taacatgatc gactatttct cgggtaagat cgacaagtcg
    13021 ccgcgcaagg agttctggta cttcaacgat gacgcccagg tcgtggcgat tcgcctcggc
    13081 gactggaaaa ccgtgttcta cgagaaccgc ggccgccagt tcggtgtctg gatggagccc
    13141 ttcaccgagc tgcgcatccc actgctgttc aacctgcgtc gcgatccttt cgagaaggcg
    13201 cagcacaact cgaacactta ctacgactgg ttcctcgacc ggccctacat catcgtgccg
    13261 atgcaggccg cggcggcgaa gttcggccag acctttatcg agtacccgcc aagtcagaaa
    13321 ccggggtctt tcaacatcag cgcgatcgtc gagaagctca agcaacagct cggctcgcat
    13381 taatcacggc aggttccggt ggccggtcgc aagcgtccgg ccaccggtgc atttttcccg
    13441 ccggcctgaa cttgtttgcg gcttgcgccg ccgtcacaat tgacggggac agttttacgg
    13501 gagttcgcaa tgcacatcta ctcgaggata tattcctcct ctctggccgc catccttgcg
    13561 ctcgttgttt tttcactgcc ggttttgtct gcgagcgacg tgccgaattc gaaaccgcgc
    13621 ctgatcctgc aaatcaccgt tgacgcgctg cgcgcggact tgccgcagcg ctactcccac
    13681 ctgttcggcg aaggcggttt ccgctacctg atggaagacg gcgtcaattt caccaacgcc
    13741 cattaccggc acgccaatac cgaaaccatt gtcggccatg tgtcgctcgc caccggcacg
    13801 gtgccggccg cgcacggcat gatcggcaac gtctggttcg accgcaagca gaggcggctg
    13861 gtctacaaca tcgaggacgc cgattaccgc ctgctcacct cgggtgccga cgtcgacagc
    13921 actaccgaga tcgacccgac gcaaaaggcg gccaaggtgg aaggccgctc gccggcggca
    13981 attctggcgc cgaccttcag cgatgagctg gcgctggcca ctgccggcaa ggcgctaatt
    14041 tttggcgtct cggtcaagga ccgcggcgcg gtatcgctgg ccggccatgc cggcaaggcc
    14101 ttctggtttt cgaagtcggg cggcgagttc gtgaccagca attactatta ctcgaagtat
    14161 cccgactggg tgaacgcgtg gaacgcgcgc aagccggcac tggcctatgc cggcggagcc
    14221 tggcagctaa gccacgagcc ggctgcctat cgctacggcg atgccgacga ccgcgattac
    14281 gagaccgact tcccgggatt cggcagaacc tttccgcatg cctacggcgc ggccgatgac
    14341 aagtatttca ccacgcgact cacgctgagc ccggccggcg acgagctgac gctcgatttc
    14401 gtcaagaccc tgctcgacaa cgagccgctc ggcgcggacg agattcccga ctacctggcg
    14461 gtgagttttt cgtcgaccga ttacgtcggc catctgttcg gcgcctccag cctggagagc
    14521 gaggacaaca tcgcgcgcct cgaccgcacg ctggcacagc tgatcgatta tatcgacgag
    14581 aaaatcggca tcgagaatac gctgatcgtg ctgtcggccg accacgggca gcccgaggca
    14641 cccgggcatt tgcacgaact cggcattcac gacgcgcatt atttcgatac cagggggctc
    14701 gacaaaaagc cggcgatcgc cgcgctgaaa cgccgcttcg gcgtcggcga ggaactgatc
    14761 gaggcgtttt ttcagcccta cgtatatctc aaccgagaac tgatcgccga caaggggctc
    14821 gaccagacgc aggtcgaagc cgccgttgcc gccgagctgg tgcgtttcga cggcgtcgct
    14881 gcggcggtct ccagttccgc gctgcgcggc gcgaacctgc cggataccga gttgaaccgc
    14941 gcgatcctga gtaatttcca cgccgcgcgt tccggcgaca tctacctggt tttcgagccg
    15001 aacgtgttca tcaacgattt cgacggcctg tcggtggcct cgacccacgg ctcgccgtgg
    15061 cgctacgaca cccacgtgcc ggtgatcttc gccggtggcg ggctgcagcc gcgcatggtg
    15121 agccgcgccg tcacccccta cgatatcgcg ccgacgctat ccggcctgct cggcatcagg
    15181 cagccagcgg caacggtcgg caacccgctg cccgaagtgc tcggttactg aatctccacc
    15241 gggccgcgcg cggcttgcgc gggaatgact gtggcatatg tcatcccgcg atctcggcgt
    15301 agaggtcttc ccaacccgga tttgaggatt cgatcaactc gattttccag cttcggtgcc
    15361 atttcttgag ttgccgttcc cgggtaagcg cggagtacat gtcgacatgc gtttggtgcc
    15421 ataccagccg atccgtgttg tatcgacttg aaaagccgtc gaccagtttg tttctgtgct
    15481 gccacacgcg ttgcgggaga ttcgtggtca caccgatgta aagaacaccg cggggtcgat
    15541 tggtgaggat gtatacacag ggggttaagg ggtccatgtc ttcgtccttg aagagaatgt
    15601 cgccgggaat tataccacca ggaatttgga gattcccgct tgcgcgggaa tgacgccaag
    15661 ggacgggaat gactccaggg tgcgggaatg actccaaggg acgggaatga ctccagggtg
    15721 cgggaatgac tcctgttttg tgtcatcccc gcgcaagcgg ggatcttgca aaggcggtgt
    15781 agtgggagat tcccgcttgc gcgggaatga ctccaaggga cggaaatgac tgcagggtgc
    15841 gggatcaggt gtcgcggtgg cggccggggc gcattgcgcg gtagaccagc accaggaaca
    15901 gcaggccgcc gatgatcgac accaggccgc cgaaacccat caggcccatg ccggcgatct
    15961 gctcgatgcc gcgctcggcg tcggtcgact tgcgcgccac cgcataaccg ccagaccagg
    16021 cgagaccggc gatgtgcagc agctgtccgc cgccatagat agcaggctgc cagcgcgcgg
    16081 cacgaccctc gaccctgcgg aagccgagct gcggcagcaa atggtaagtc agccccatga
    16141 aggcgatcgt gatgccgacg atcgagccgt ggtag
//
LOCUS       DATCZV010000499         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_624022, whole genome shotgun sequence.
ACCESSION   DATCZV010000499 DATCZV010000000
VERSION     DATCZV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624022"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VIW27_11275"
     CDS             <1..242
                     /locus_tag="VIW27_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY5740300.1"
                     /translation="RGREGGLAGAAGEVGLDDGSQLSPKGRQWIPDGRRLVVRLPGGG
                     GYGAPAARDRALVEQDLTREYISAARAADDYDYRK"
     gene            complement(257..1480)
                     /locus_tag="VIW27_11280"
     CDS             complement(257..1480)
                     /locus_tag="VIW27_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5740301.1"
                     /translation="MTAALATGIGGSSAASELLRFQSRRAAAIPIGAAEYRERVERLR
                     AGMRESGVDAVFLCASTSLYYFTGLRCRPSERLHGAILRQSGEPLYVCPAFEEQKTRA
                     EIVLEGDFLLWEEHDNPTRAIVDSLSKCLPGGARLALDEQTPFFIASRLQESATGLQI
                     INAENLIAACRRIKSPAELALLAQAKAITLRVQQAAARILHEGIDTREVQAFLDAAHI
                     AAGMDGRSTFNIVLFGEATAYPHGVPYPQQLREGDMVLIDTGATLHGYNSDITRSYVF
                     GEADARQREIWELENAAQAAAFAAARIGAPCSAADDAARAVIEAAGFGPGYSLPGLPH
                     RTGHGVGLDVHEQPYIVRGNDLPLQAGMCFSIEPMICSYGEFGVRLEDHVYMTDDGAR
                     WFTEPSAAIDDPFGG"
     gene            1659..3239
                     /locus_tag="VIW27_11285"
     CDS             1659..3239
                     /locus_tag="VIW27_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740302.1"
                     /translation="MKFRLIAVAVMFLFAAGAQAKTLIFCSEGSPEGFTPSLYTAGTT
                     FDASSRQIYNRLAEFELGTTKIIPGLAESWEVSDDGLTYTFNLRRGVKFQTTKNFTPS
                     RDFNADDVIFSFMRQFDKSHPYHLVSGGAYEYFNGMSMPDLINKIEKIDDYTVRFTLN
                     RPEAPMIANLAMDFASIHSAEYADKMAAAGTLDAFDQNPVGTGPFQLVNYQKDAFIRY
                     QAHPQYFRGKAAIGDLVFAITPDASVRYQKLKVGECHVMPYPNPADIEAMRKDPKVNL
                     MSQEGLNVGYLAYNTKMSPFDNAQVRKALNMAINKEAIIDAVFQGAGKVAKNPIPPTI
                     WSYNEATVDDPYDPAKAKAALEAEGVKGLKMKIWAMPVQRPYNPNARRMAELIQADFA
                     EVGVDAEIVSYEWGEYLKRSKAEDRDGAVLLGWTGDNGDPDNFLAVLLGCDGVGGSNR
                     AQWCYQPFEDVIQKAKVVSDVSERTRLYEQAQIEFKSQAPWATIAHSVVFKPVRKEVT
                     GFKIDPFGGHSFYGVDLK"
     gene            3314..>3627
                     /locus_tag="VIW27_11290"
     CDS             3314..>3627
                     /locus_tag="VIW27_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950286.1"
                     /note="transports peptides consisting of two or three
                     amino acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HEY5740303.1"
                     /translation="MLKFLFTKLAMIVPTFIGVTIASFAFIRLLPGDPILLMAGERGV
                     TPERRAELERIMGFDRPLWEQYFSFLGDLLQGDLGVSIVTKAPVMEEFLGRFPATVEL
                     SV"
BASE COUNT          711 a         1099 c         1144 g          673 t
ORIGIN      
        1 cgcgcgggcg cgaaggcggg ctggcaggag ccgccggcga ggtcggtctc gacgacggca
       61 gccagctgtc gccaaagggc cggcaatgga ttcctgacgg ccgtcggctg gtggtcaggc
      121 tgcccggcgg cggcggttac ggcgctcccg cggcgcgcga cagggccctg gtcgaacagg
      181 atctgacgcg cgaatatatc agcgcggcgc gggcggcgga cgattacgat taccggaaat
      241 agtcctcgcg gagccgctag ccgccgaacg ggtcgtcgat ggctgcgctt ggctcggtaa
      301 accagcgcgc gccgtcatcg gtcatgtaaa cgtgatcctc gaggcgtacg ccgaattcac
      361 cgtaactgca aatcatcggc tcgatggaaa agcacatgcc ggcttgcagt ggcagatcgt
      421 tgcccctgac gatgtaaggc tgctcgtgca cgtcgagtcc gacgccatgg ccggtgcgat
      481 gcggtaagcc gggcaaactg taaccgggcc cgaaacccgc cgcttcgatc accgcgcggg
      541 cggcgtcgtc ggcggccgag cagggcgcgc cgatgcgcgc ggccgcgaag gccgcggcct
      601 gggcggcatt ctccagctcc cagatttcgc gttgccgcgc gtcggcctcg ccgaatacgt
      661 agctgcgggt aatgtccgag ttgtagccgt gcagcgtcgc gccggtgtcg atcagcacca
      721 tgtcaccctc gcgcagctgt tgcgggtagg gcacgccgtg cggataggcg gtcgcctcac
      781 cgaacagcac gatgttgaag gtcgagcggc cgtccatccc cgcggcgata tgcgcggcat
      841 cgagaaacgc ttgcacctcg cgcgtatcga ttccctcgtg caggatcctt gccgccgcct
      901 gttgtactcg cagcgtgatc gccttggcct gcgcgagcag cgccagttcc gccggcgatt
      961 tgatgcggcg gcaggcggca atcaggttct ccgcattgat gatctgcaac ccggttgccg
     1021 attcctgcag gcggctggcg atgaaaaacg gggtctgctc gtcgagcgcg agcctggcgc
     1081 cgccgggcag gcacttcgac aggctgtcga cgatcgctcg cgtcgggttg tcgtgctctt
     1141 cccagagcag gaaatcgccc tcgagaacga tttccgcacg ggttttctgt tcctcgaacg
     1201 cggggcagac gtaaagcggc tcgccggatt gccgcaggat ggcgccgtgc aggcgctcgc
     1261 tggggcgaca cctgaggccg gtgaaatagt agaggctcgt cgaagcgcac aggaaaacgg
     1321 catcgacgcc cgattcgcgc attccggcgc gcaggcgctc gacacgctcg cggtattcgg
     1381 ccgcgccgat cggaatcgcc gcggcgcgcc gcgattggaa acgaagtagt tcgcttgccg
     1441 cgctggaccc gccgataccg gttgcgagcg ccgccgtcat cgctgtgcag cgcaaaagat
     1501 ttgcgaattg tcttgcatca ctttgtcgtt ataatggccg acggccaacc gcgttaatca
     1561 agatcggtaa taatacacga gggaagccga agaataatcg atcgacgata gacaaaataa
     1621 tcatcccgca accgggagcc aaccgaagag gtagcatcat gaaattcaga ctaatagcgg
     1681 tcgccgtaat gtttttgttc gcggcgggcg cgcaggcgaa aacactcata ttctgttccg
     1741 agggctcacc cgagggattt actccgtcgt tgtacaccgc gggcacgact ttcgacgcgt
     1801 cgtcgcggca gatctacaac cggctggccg aattcgaact cggcacgacc aagattattc
     1861 cgggtctcgc cgagagctgg gaggtatcag acgacggcct gacctatact ttcaacctgc
     1921 gccgtggcgt taagtttcag accaccaaga actttacccc gagccgcgac ttcaatgccg
     1981 atgacgtgat cttttcgttc atgcgccagt tcgacaagtc tcatccctat cacctggtct
     2041 cgggcggcgc ctacgagtat ttcaacggca tgtcgatgcc ggacctgatc aacaagatcg
     2101 aaaagatcga cgattacacc gtgcgcttta cgctgaaccg gcccgaggcg ccgatgatcg
     2161 ccaacctggc gatggatttc gcgtcgatcc actcggccga gtacgccgac aagatggccg
     2221 ccgccggtac cctggacgct ttcgatcaaa acccggtcgg caccggtccg ttccagctgg
     2281 tcaattatca gaaagatgcc ttcatccgct accaggcgca tccgcagtat ttccgcggca
     2341 aggcggcaat cggtgatctg gtgttcgcaa tcacgccgga cgcatcggtt cgctaccaga
     2401 agctgaaagt cggtgaatgc catgtcatgc cctacccgaa cccggccgat atcgaggcga
     2461 tgcgcaagga tccgaaggtc aacctgatga gccaggaagg cctgaatgtc ggttacctcg
     2521 cctacaatac gaaaatgtcg ccgttcgaca acgcgcaagt gcgcaaggcg ctgaacatgg
     2581 cgatcaacaa ggaagcgatc atcgacgcgg tattccaggg tgcgggtaaa gtagccaaga
     2641 acccgattcc gccgaccatc tggtcgtaca acgaagccac ggtcgacgat ccctatgatc
     2701 ctgcaaaagc gaaagccgcg ctcgaggccg aaggcgtcaa gggcctgaaa atgaaaatct
     2761 gggcgatgcc ggtacagcgt ccctacaacc cgaacgcgcg ccgcatggcc gaactgatcc
     2821 aggccgattt cgccgaggtc ggtgtcgacg cggaaatcgt ttcctacgag tggggtgagt
     2881 acctgaagcg ctccaaggcg gaagaccgtg acggcgcggt actgctgggc tggaccggcg
     2941 acaacggcga cccggacaac ttcctcgcgg tgctgctcgg ttgcgacggt gtcggcggat
     3001 ccaaccgcgc gcaatggtgc taccagccgt tcgaggacgt gatccagaaa gccaaggtag
     3061 tatccgacgt ctccgagcgt acccgtctct acgagcaggc gcagatcgag ttcaagagcc
     3121 aggctccgtg ggcgacgatc gcgcattcgg tggtattcaa gccggtacgc aaggaagtta
     3181 ccgggttcaa gatcgatcct ttcggcggtc actcctttta cggggtcgac ctgaaatagc
     3241 cgcttacgga aaggccccac ccgcgtgggg cctttcacgt ttcagagctg aggcggggtt
     3301 tcggcggtag ccgatgttaa aatttctatt caccaaactg gcgatgatcg tgccgacttt
     3361 catcggcgtg accatcgcgt cgttcgcgtt cattcggctg ctgcccggtg acccgattct
     3421 gttgatggcc ggtgaacgcg gcgtgacccc cgagcggcgc gccgagctcg agcgcatcat
     3481 gggcttcgac cgaccgctgt gggagcaata tttcagcttc ctcggcgacc tgttgcaggg
     3541 cgatctcggt gtttccatcg tcaccaaggc gccggtgatg gaggagttcc tggggcgttt
     3601 tccggccacc gtggagttgt cggtgat
//
LOCUS       DATCZV010000500         2380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_358757, whole genome shotgun sequence.
ACCESSION   DATCZV010000500 DATCZV010000000
VERSION     DATCZV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358757"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VIW27_11295"
     CDS             <1..453
                     /locus_tag="VIW27_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5740304.1"
                     /translation="VRRGPADVQDPKINSHSKLNCILACIQAAKAGADEALMLDPHGF
                     VATCNSTHFFIVRGGEVWTSSGDYCLGGITRGNVIALCERNGIPVRQKNFSLTDVYGA
                     AEAFVTGTFAGLVPVREVDGRRIGDGKRGAMVERLQRLYREMLEDECR"
     gene            450..1181
                     /locus_tag="VIW27_11300"
     CDS             450..1181
                     /locus_tag="VIW27_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5740305.1"
                     /translation="MSTLRIAMWSGPRNISTALMRAWESRTDCAVWDEPLYGYYLART
                     AIDHPGAAEIIAAHGSDPEQIIARCIGDPPGGKSIFYQKHMTLHLLHELDRGWLEALA
                     NCFLIREPEPVIASYTAVRERATLDDIGFAQQAALFEQVCEQSGDVPPVIDSREFLLD
                     PESMLRALCARLDVDFEPSMLSWPPGPRDSDGVWGKYWYASVWDSTGFAPYRQKRLTL
                     DERDREIAERARPYYELLYRHRLRP"
     gene            1295..>2380
                     /locus_tag="VIW27_11305"
     CDS             1295..>2380
                     /locus_tag="VIW27_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5740306.1"
                     /translation="MAEPQLLTPAPGSRIVKDEVEVILFGQPPEVLKGLLREGITGFD
                     TLVLTDIREKDSALLNNLEFPLYFFLFFARGLKENRKLNLVGHADAISHAMRLLRFTL
                     LGPTRAELDRWHTDSELKEEWLAVSEALALKNDSGKIVSVEQFFNPIPFADNLAVAGG
                     FVIEHLGVDRYLVTNAGGDVHVDLNDGVAVRPPYPVPIDYVPGGLSKMGLEVLGGASG
                     FSTEEPCTGLALCYNGDYLLIDSIPFLDQQLFARGISKNQVSAVFLTHLHDDHCSMFP
                     LMEMPHCVEVITTREIFNMAMEKLSCGLGWSVDVVREHFKLLEVKPGGTLNYYGLSIE
                     VHNTVHSIPTIGATFATVHKGQFRDVCI"
BASE COUNT          466 a          754 c          698 g          462 t
ORIGIN      
        1 gtgcgccgcg gccctgcgga cgtgcaggat cccaaaataa acagtcactc caagctaaac
       61 tgcattctcg cctgcattca ggcggccaag gcgggcgccg acgaggcgct gatgctcgat
      121 ccgcacggct tcgtcgctac ctgcaactcg acgcattttt tcattgtccg cggcggcgag
      181 gtttggacct cgagcggcga ctactgcctc ggcggcatca cccgcggtaa cgtcatcgcg
      241 ctgtgcgaac gcaacggcat cccggtgcga cagaaaaatt tcagcctgac cgacgtctat
      301 ggtgccgcgg aagctttcgt caccggcacc ttcgccgggc tggtaccggt gcgcgaagtc
      361 gacggccgcc gaatcggcga tggcaagcgc ggcgcgatgg tcgagcgctt gcagaggctt
      421 taccgcgaaa tgctcgagga tgagtgccgg tgagcacgct gcgtatagcg atgtggtcgg
      481 ggccgcgcaa catttcgacc gcgttgatgc gcgcctggga aagccgcacc gattgcgcgg
      541 tctgggacga accgctgtat ggctactacc tcgcgcgcac cgctatcgat catcccggcg
      601 cggccgagat tatcgccgcg cacggcagcg atcccgagca gatcatcgcc cgctgcatcg
      661 gcgatccccc cggcggcaaa agcatctttt accagaaaca catgaccctg cacctgctgc
      721 acgagctcga tcgcggctgg ctcgaagcac tcgcgaattg ctttctgatc cgcgaacccg
      781 agccggtgat cgcttcctac accgcggtgc gcgagcgcgc caccctcgac gacatcggct
      841 tcgcgcaaca ggccgcgtta ttcgaacagg tgtgcgagca gagtggcgac gtgccgccgg
      901 tgatcgacag ccgcgagttc ctgctcgatc ccgagtcgat gctgcgcgcg ctgtgcgcgc
      961 gtctcgatgt ggatttcgag ccgagcatgc tgagctggcc gccgggtccg cgcgacagcg
     1021 acggcgtgtg gggcaagtac tggtatgcat cggtctggga ctcgaccggc ttcgcgccct
     1081 accggcaaaa gcggctgaca ctcgatgaac gggatcgcga aatagccgaa cgcgcgcggc
     1141 cttattacga actgttatac cggcatcggt tgcggccctg aacgccaata ccgcgaatgc
     1201 cggtcacttt gctgcaaaaa atctaacaaa ctttcacctt tcacccccgc aacgggcggt
     1261 atacacttcg ccatcagcgt taataattcg gacaatggct gaaccacaac tgcttactcc
     1321 cgcacccggc agcagaatcg tcaaggatga agtcgaagtt attcttttcg gtcagccacc
     1381 cgaggtactg aaaggcctgc tgcgagaggg cattaccgga ttcgacacgc tggtgctgac
     1441 cgatattcgc gaaaaggaca gcgcgctgct caacaacctc gaattcccgc tctatttttt
     1501 tctgtttttc gccaggggtc tgaaggaaaa ccgcaagctc aacctggtcg gccatgccga
     1561 cgccatcagt cacgcgatgc gtctgttgcg tttcaccctg ctcggaccga cccgcgcgga
     1621 actcgatcgc tggcataccg acagcgagct gaaagaggaa tggctggccg tctccgaggc
     1681 actggcgctg aagaacgaca gcggcaagat cgtcagtgtc gagcaattct tcaacccgat
     1741 cccgttcgcc gacaacctcg cggttgccgg tggctttgtc atcgagcacc ttggtgtcga
     1801 ccgttacctg gtcaccaatg cgggcggtga cgtacatgtc gatctcaacg acggcgtcgc
     1861 ggtcaggccg ccctacccgg taccgatcga ctatgttccc ggtggcctga gcaagatggg
     1921 cctcgaggta ctcggcggcg cttccggctt ttccaccgag gaaccctgca ccggactcgc
     1981 actgtgttac aacggcgact acctgttgat cgattcgatt ccgtttctcg accagcaact
     2041 gttcgcgcgc ggcatctcga agaaccaggt gtcggcggtg ttcctgaccc atctgcacga
     2101 cgaccattgc tcgatgttcc cgctgatgga gatgccgcat tgcgtcgagg tcatcaccac
     2161 ccgcgaaatc ttcaacatgg cgatggaaaa gctgagctgc ggtctcggct ggtcggtgga
     2221 cgtagttcgc gagcatttca agctgctcga ggtcaagccc ggcggcacgc tcaattacta
     2281 cgggctcagc atcgaggtac acaacaccgt gcacagcatt cccaccatcg gcgcgacctt
     2341 tgccacggta cacaagggtc agttccgtga cgtttgcatc
//
LOCUS       DATCZV010000501         5993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_624070, whole genome shotgun sequence.
ACCESSION   DATCZV010000501 DATCZV010000000
VERSION     DATCZV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624070"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VIW27_11310"
     CDS             <1..601
                     /locus_tag="VIW27_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY5740307.1"
                     /translation="HMPEMDGLTAATEIQQRYSPPQRPRVVPMTANVLPGDREACGEA
                     GMQDFLAKPIDIDHLYRILCNLDPGPSREIPADVTDHAAADIDFERLDMIRETPSDGD
                     QNLLAWVIDTFLEDLDERLPRMRDLAERADWKALAEEAHRLYSSASNLGLVRISNICM
                     QLELEAANAEVDKVGLVQELVAAWQSVLPELERQKALDQ"
     gene            1222..2673
                     /locus_tag="VIW27_11315"
     CDS             1222..2673
                     /locus_tag="VIW27_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5740308.1"
                     /translation="MIAFTVLTIYAAGLTLFVLDQKEALIDEVEQLRYVYEREGSVSQ
                     SDLATLRTLQNLRAEILVQKDDFDKEGLREQLALVRSLYEGLPMRYPGGETTLLSLQS
                     ALDEAYINPTLASVSALRFSLQDAEEMLGRRIDQIRQQQSSRFDGFRERSNWVAVAAL
                     LIGIMGLMAIGITSGRFLKTISRDLKSLELQTAAVVKRDSPPADLHDKDLPRRGDEVG
                     KLSEALERLSLELEERERRSEIEHEKYIHQEKTAAIGTLAAGIVHEIGNPVASIAELV
                     RSVRDEGDAPHALDREEIHQRCDAVLQHLERILAVVRDVSEFSTNPSGERELLDLNQL
                     IRTTYRLMRYDKRLLKVGCALELDKNLPPLEGNEEQLIQVIMSMLVNAADAVEGLDSR
                     VPVITISTYAEQDRIHLVIRDNGCGMDDEAQRHVFDAFYTTKAVDRGTGLGLTLCQSI
                     VDDHGGTIEIESQADAGTTIHIYLPHGPLESES"
     gene            2846..3523
                     /locus_tag="VIW27_11320"
     CDS             2846..3523
                     /locus_tag="VIW27_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5740309.1"
                     /translation="MLSPEHRDEFERLFPFVAQAGADFKRDFYAACRYLELPADVSIC
                     DEGQQCAQLALLLDGVGRVYKLSPAGREVTLYRIEAGEACVLTASCIINGASFPAMAV
                     TETPVRAIVVSPQKVRGWICAEAQWQQFIFGLLSHRLASIISVVEEVAFKRIDVRLAE
                     KFARCLARGESSLQVTHAELAADLGSSREVVSRILRDFAQRGLIETRRGSILLADRQA
                     IEQLSRQ"
     gene            3630..3854
                     /locus_tag="VIW27_11325"
     CDS             3630..3854
                     /locus_tag="VIW27_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY5740310.1"
                     /translation="MQKNVGSIDRTIRAVVGIALLAAYFLGAVQGTLGIVALVVGIVM
                     LGTAALGWCPPYSLLGINTCGVKQDPKAPA"
     gene            complement(4083..5201)
                     /locus_tag="VIW27_11330"
     CDS             complement(4083..5201)
                     /locus_tag="VIW27_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY5740311.1"
                     /translation="MSRSTPQQIRARDLGIPFTGEPGAVNAITDVPGVRVGYRTLVED
                     LAADADGPVAVRTGVTAILPRAPENLLEPTWAGVASFNGNGELTGAHWIAESGTFTGP
                     IMLTNTHSVGMVHHACLQWLTRRPEYDAARDPWLLPVVAETCDAVLNDINGFHVSAAH
                     VFAALDSAAGGAIAEGNVGGGTGMRCYEYKGGTGTASRVLALGDRRYTLGALVQANFG
                     RREELSIRGVPVGQLAGPAANADGDSGSLIVVIATDAPLLPLQLQRIARRCTLGMGRT
                     GTSGNNGSGDLFIAFSTSRSTPSGAGDPVESMSLLPAEALDPLFRATVESTEEAIVNA
                     LVAAETISGHRGRKLESIDREWLASLCAESPTGRPNST"
     gene            5550..>5993
                     /locus_tag="VIW27_11335"
     CDS             5550..>5993
                     /locus_tag="VIW27_11335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY5740312.1"
                     /translation="MSKLENVNIKSEQLLITPQHLKLMLPVPADTADRVQRAREAVKA
                     ILRRDDPRLLVIVGPCSIHDPKAALEYAARLAKLVRELDDSLLLVMRAYFEKPRTTVG
                     WKGLINDPHLDDTFEIAEGLEIARRLLLDIAEMDLPLSTEALDPIT"
BASE COUNT         1119 a         1814 c         1922 g         1138 t
ORIGIN      
        1 gcacatgccg gagatggacg gattgacggc ggcaacagaa attcagcagc ggtactcacc
       61 gccgcagcgg ccgcgggtgg tgccgatgac cgccaatgtg ttgcccggcg atcgcgaggc
      121 ctgcggcgaa gccggcatgc aggactttct cgccaagccg atcgatatcg atcacctgta
      181 tcgtatcctc tgcaacctcg atcccggccc gagccgcgaa atcccggcgg acgtcaccga
      241 ccatgcagcc gcggatatcg acttcgaacg gctcgacatg atccgcgaaa cgccgagcga
      301 cggcgatcag aacctgctgg cctgggtgat cgataccttc ctcgaggacc tcgacgagcg
      361 cttgccgcga atgcgagatt tggccgagcg cgccgactgg aaagccctgg ccgaggaagc
      421 ccaccgtctg tattcgagtg cgagcaatct gggactggtc agaatctcga atatctgcat
      481 gcaactggaa ctcgaggctg ccaatgcgga ggtggacaag gtcgggcttg tgcaggagct
      541 ggtggcagcc tggcagtcgg tcctgccaga gctcgagcgg cagaaagccc tggaccagta
      601 aagctgtcga attccctctc gcctctggcg cgccggcgcc gtggcatgga atcagtttcg
      661 cggtttcggc atgtcgaaat cgcagctttt cacgccagcg cgcggaccag atagacgacg
      721 accgcgattg ccgcggcgca cagcatacag gcgaaggcga gtttggaggc gaggatagct
      781 ttttcgttca tcgtttcagg gatccgtggc cagggttgtg agcggcgctg atgcaattgc
      841 gcggatttcg ctgcggcggg ccggcaccgg cgaaataaac gacatcgaat cgacacgcgt
      901 ttctcgaatt cgagaggtgg tttctctcaa aattgagaga aaaccaccct ttccgggttc
      961 cgacggtctg aaagtgtccg ggaattgagg gtttgcagtt tcgaaaatcg aattatttct
     1021 acagttttcc gcgtttttct ccgatcaggc cggtaattta gtcgataaat gcggcaaatc
     1081 gcgtgccagt tcggccgaac gcgaatagtt ggcgcaaccc ttgcatgtaa aaccgctaag
     1141 gcaggattcg gcgaggggct gaattatcgt ggcggaaagg caaacatcat gatgagcgga
     1201 atgtcgcttc aggcgaaggg catgatcgcg tttacggtgt tgacgatcta tgccgcgggt
     1261 ctgacgttat tcgtgctcga tcagaaggag gcattgatcg acgaggtcga acaattgcgc
     1321 tatgtctacg agcgcgaagg ttccgtatcg cagtccgacc tcgctaccct gcgtacgctg
     1381 cagaatctgc gcgccgaaat cctggtacag aaagacgatt tcgataagga gggtctgcgc
     1441 gagcaactgg cgctggtccg ttccctctac gaaggcttgc cgatgcgcta tccgggcggc
     1501 gagacgaccc tgttatcgct gcagagcgcg ctcgacgaag cctatatcaa cccgaccctg
     1561 gccagtgtct cggcgctgcg cttcagcctg caggacgccg aggagatgct cggccgccga
     1621 atcgaccaga tccgtcagca gcagagcagt cgtttcgatg gtttccgcga gcgcagtaac
     1681 tgggtggcgg tcgccgcgct gttgatcggt atcatgggtt tgatggccat cggtatcacc
     1741 agcggccgct tcctcaaaac gattagccgc gatttgaaaa gcctggaact gcagacggca
     1801 gccgtagtca agcgcgacag cccgccggct gatttgcacg acaaggatct gccccggcgc
     1861 ggcgacgaag tcggcaaact ttccgaggcc ctggagcggt tgtcgctgga actcgaggag
     1921 cgtgaacggc gcagcgagat cgaacacgaa aagtatatcc accaggaaaa aaccgccgcg
     1981 attggaaccc tggccgccgg cattgtgcac gagatcggca atcccgttgc ctcgatcgcc
     2041 gaactggtgc gcagcgtgcg cgacgagggc gatgcgccgc atgccctcga tcgggaagaa
     2101 atacatcaaa ggtgcgatgc cgtcctgcag cacctggagc gaatcctcgc ggtggttcgc
     2161 gacgtgtcgg agttttccac caatccgtcc ggggagcgcg aactgctcga tctcaaccag
     2221 ttgatccgca ccacctaccg gctgatgcga tacgataagc ggctgctgaa ggtcggctgc
     2281 gccctcgagc tcgacaagaa tctgccgcca ctggagggca acgaggaaca gctgatccag
     2341 gtcattatga gcatgctggt caatgcggcc gacgcggtgg aagggctcga cagccgggtg
     2401 ccggtgataa ccataagcac gtatgccgag caggatcgca tacacctggt cattcgcgac
     2461 aacggctgcg gtatggacga cgaggcgcaa agacacgtgt tcgatgcgtt ttacaccacc
     2521 aaggcggtcg accgcggaac cgggctgggt cttaccctct gccagtcgat cgtcgacgat
     2581 catggcggca cgatcgagat cgagtcgcag gccgacgcgg gtaccacgat ccatatatac
     2641 ctgccgcacg gcccgctgga gtcggaatcc tgaaccgata ggcgccggtt cgctcgcctc
     2701 gcgcctgttt gctgaatccc ttgaaaaccc gctgccgcag caaatgtttc ggccggcggt
     2761 aacaatttct gattgacggt ttgaatcgcc cgtggcgagc aggcactatt gtcaatgtcg
     2821 cccgcggaga gtccaccagt ttgctatgtt gtcgccggag catcgagacg aattcgaacg
     2881 gctgttcccc ttcgtggcgc aggccggtgc cgatttcaag cgggatttct acgcggcctg
     2941 ccgttacctc gagctgcccg ccgatgtttc catctgcgac gagggccagc agtgcgcgca
     3001 gctggcgctg ctgctcgacg gcgtcggccg ggtgtacaag ctgtcgccgg ccgggcgcga
     3061 ggtgacgctg taccgtatcg aggccggcga ggcctgcgtg ctgaccgctt cgtgcatcat
     3121 caatggcgcc agtttcccgg cgatggcggt gaccgagacg ccggtgcgcg cgatcgtcgt
     3181 ctcgccgcaa aaggtgcgtg gctggatctg cgccgaggca cagtggcagc agttcatctt
     3241 cggcctgttg tcgcaccgcc tggccagtat catctcggtg gtcgaggaag tcgccttcaa
     3301 gcgaatcgat gtacgcctgg cggaaaagtt cgcccgctgc ctggcgcgcg gcgagagcag
     3361 cctgcaggta acccacgccg aactggccgc cgacctcggc agttcgcgcg aggtggtcag
     3421 ccgaatactc agggacttcg cgcagcgtgg cctgatcgaa acccgccgcg gcagcatcct
     3481 gctcgccgac cggcaggcga tcgagcagct gtcgcgccag taatgttcgc ccagcgggtt
     3541 gcaagcggcg tcgcgcttgc gtgactttgt cactgacgcg gcgcttgcgc aggcctatac
     3601 tgcgcacatc ttaaacagga gcaagtatca tgcaaaagaa tgttggtagt atcgatcgga
     3661 cgattcgcgc cgtagtcggc attgcgctgc tggcggcgta tttcctcggc gccgtgcagg
     3721 gcacgctggg catcgtggcc ctggtggtcg gcatcgtcat gctcggcacc gctgccctcg
     3781 gctggtgccc gccttactcg ctgctgggta tcaatacctg cggcgtcaag caggacccga
     3841 aagccccggc ctgagccgga cgctgtttta gcgggcgccg gcctgttgcc gcaggccggc
     3901 gatcggtgcc cgttagcggc tcgcgcatgc agcgctgagg cgtctttcgt tatacgggca
     3961 tgatcgcagc cggaatcggc tattccgccc tgctgcgcac cgcgagcatc ggcctgcggt
     4021 ttgcgggggc cgccaaatcc aactactttg ccgctatcct cggtgtccgc ccgggaaagg
     4081 gttcaggtcg aattcggccg gcccgtcggg gattcggcac acagcgaggc gagccattca
     4141 cggtcgatcg actcgagctt ccgcccgcga tgcccgctga tggtctcggc cgcgaccagc
     4201 gcgttgacga tcgcctcctc ggtactttcc acggtggccc ggaacagcgg gtcgagcgct
     4261 tccgccggca gcagtgacat cgactcgacg ggatcgccgg cgccgctcgg cgtgctccgc
     4321 gacgtgctga aagcgatgaa caaatccccc gatccgttgt tacccgaagt gccggtgcgc
     4381 cccatgccga gcgtgcagcg gcgcgcgata cgttgcagct gtagcggcag caatggtgca
     4441 tcggtcgcga tcaccacgat cagcgagccg gaatcgccgt cggcgttcgc tgccggaccg
     4501 gcgagctggc ccaccgggac accgcgaatg ctgagttcct cgcggcggcc gaaattggcc
     4561 tgtaccagcg cgccgagggt atagcgccgg tcgccgagcg ccagcacccg cgacgcggtc
     4621 ccggtgccgc ccttgtactc gtagcagcgc atgccggtgc cgccgccgac gttgccctcg
     4681 gcgattgcgc cgcccgcggc actgtccagc gccgcgaaca cgtgcgccgc gctgacgtgg
     4741 aagccgttga tgtcgttcaa caccgcgtcg caggtttcgg ccaccaccgg caacagccac
     4801 gggtcacgcg ccgcgtcgta ctccggtcgt cgcgtcagcc attgcaggca ggcatgatgg
     4861 accatgccga cgctgtgcgt attggtcagc atgatcgggc cggtgaaggt gcccgactcg
     4921 gcgatccagt gtgcgccggt cagttcgccg ttgccgttga aggatgcgac gccggcccag
     4981 gttggttcga gcaggttttc gggcgcacgc ggcagaatcg cggtcacccc ggtgcgcacc
     5041 gcgaccggcc cgtcggcatc ggccgcgagg tcttcgacca gggttctgta gccgacgcgt
     5101 acgccgggca catcggtgat cgcgttcacc gcgccgggct cgccggtaaa cggaatcccg
     5161 agatcgcggg cacggatttg ttgcggcgtg gatcgggaca tggtgttggt ctcccaggtt
     5221 gtaagcggta tttgggtctg tttttcgcga ttatgcgccc acagcttgcc atccgcgacg
     5281 aaatcgataa gatcggggtt cagaaatttt gcaggcagaa agagtaatcg catgcagccg
     5341 gtattcgaaa tgaatattca atatcagggc gggttcgtcg cagccccctg gtcggttgcc
     5401 tattacttta ttggttggta acactacctt ctcttcgcga aggtagtctt agctgccttc
     5461 ggtaccgaaa aaccccgtaa ggcagccagc cagcggggtt tttttattcc cgcctgtcgc
     5521 ccgactgtat atgaactaaa aggtgagata tgagtaagtt agaaaacgtc aacatcaagt
     5581 cggaacagtt gttgatcacg ccgcagcatt tgaagctgat gctgccggtg ccggccgata
     5641 ccgccgacag ggtgcagcgt gcgcgcgagg cggtcaaggc catcctgcgg cgcgacgatc
     5701 cgcgcctgct ggtgatcgtc gggccctgtt ccatccacga tccgaaggcg gcgctggaat
     5761 acgccgcgcg tctcgcgaaa ctcgtgcgcg agctcgacga cagcctgctg ctggtgatgc
     5821 gcgcctattt cgaaaagccg cgcaccacgg tcggctggaa gggcctgatc aacgatccgc
     5881 acctcgacga taccttcgag atcgccgagg gactggagat tgcgcgccgg ctgttgctcg
     5941 atatcgcgga gatggacctg ccgctgtcga ccgaggcgct ggacccgatc acg
//
LOCUS       DATCZV010000502         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_253543, whole genome shotgun sequence.
ACCESSION   DATCZV010000502 DATCZV010000000
VERSION     DATCZV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253543"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VIW27_11340"
     CDS             <1..645
                     /locus_tag="VIW27_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5740313.1"
                     /translation="GGPVAFAPMRPETTLGSVVSDAGLRQLHCAETEKYAHVTFFFNG
                     GREPPYLGETRELIESPRVETYDLKPEMSAPGVAHAVIEAMRSEQYEFIVVNFANGDM
                     VGHTAVREAIIRAVEVLDYEVGRVLEAASECGYSVVLTADHGNCDEMIDPATGKPHTQ
                     HSNHPVPCLVLDPDVRELAENGNLSAIAPTVLQLMGIEQPESMTGHSVILDRWG"
     gene            761..1372
                     /locus_tag="VIW27_11345"
     CDS             761..1372
                     /locus_tag="VIW27_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5740314.1"
                     /translation="MSAEDLENIASQTVIRQFPKNTVIVSQGDETDSFYVILQGKVDV
                     FLQNDKGKEIIINTLGECEAFGELAPLGKIPRQASIITTEDSTFGIISRQVFMDTLLT
                     KPTVSMQIIDLLVHRIQDLTEEVSSLALEDVYNRVVRVLYKHAEEVGEKLVTDKLTQQ
                     DIASRVGATREMVHRILKELKTGGYISIEGKHITIEKKLPPGW"
     gene            1426..2172
                     /locus_tag="VIW27_11350"
     CDS             1426..2172
                     /locus_tag="VIW27_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5740315.1"
                     /translation="MAGHSKWANIQHRKKAQDARRGKLFTRLIREIMVAARAGGSDLD
                     ANPSLRTAVDKAKAQSVPKDAIERAIKRGAGELEGSDYEEIRYEGYGPAGVAFIVDCL
                     SDNRNRTVAEVRHAFSKGGGNLGQSGSVAYLFDNVGVLLYSPEHDEDAVMEAALEAGA
                     EDVIVADDGSIELLTAPEQYIAVKQAMIEAGFEPEESDLTLRAQTTTELELEDAQKVL
                     RLVDLLEGSDDVQEVYYNADIPEAAYQDEA"
     gene            2173..>2464
                     /gene="ruvC"
                     /locus_tag="VIW27_11355"
     CDS             2173..>2464
                     /gene="ruvC"
                     /locus_tag="VIW27_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014948862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5740316.1"
                     /translation="MIILGLDPGSRITGFGLIDNQTDRIEYVHSGCIRVSGDSLAARL
                     GHIYAEIDAVIRQYRPRQMGIERVFMARNPDSALKLGQARGAAICAAHQAGLE"
BASE COUNT          536 a          743 c          752 g          433 t
ORIGIN      
        1 ggcgggccgg tcgcgttcgc gccgatgcgc cccgagacca cgctcggcag cgtcgtcagc
       61 gatgccggat tgcgccagtt gcactgcgcc gagaccgaga aatacgccca tgtaaccttt
      121 ttcttcaacg gcgggcgcga accgccttac ctcggcgaga cgcgcgaact gatcgaatcg
      181 ccgcgcgtcg agacctacga cctcaaaccg gagatgagcg cccccggggt cgcgcacgcg
      241 gtcatcgagg cgatgcgctc ggagcaatac gaattcatcg tcgtcaattt cgccaacggt
      301 gacatggtcg gccataccgc ggtacgcgag gcgatcattc gcgcggtgga agtgctcgat
      361 tacgaggtag ggcgtgtact cgaggccgcc agcgagtgcg gttactcggt ggtattgacc
      421 gccgaccacg gcaattgcga cgagatgatc gatccggcga ccggcaagcc gcatacccag
      481 cattccaacc acccggtacc ctgcctggtg ctcgaccccg acgtgcgcga gcttgcggaa
      541 aacggcaatt tgtccgcaat cgcaccgacc gtactacaat tgatgggcat cgagcagccc
      601 gaatcgatga ccgggcactc ggttatcctc gaccgctggg gttaattacc ttgattgcag
      661 gcgagttttt ggtgattatt gactattggc tgtgcgcgat taaataacaa atatggcgac
      721 cttaaccgaa acattagaag aaattacact gtttcgcgga atgagcgcgg aagacctcga
      781 aaatatcgcc tcgcaaaccg ttattcgcca gtttccgaag aataccgtca tcgtcagcca
      841 gggagatgaa accgattcgt tttacgtgat cctgcagggc aaggtcgacg tctttctgca
      901 aaacgacaag ggcaaggaaa tcatcatcaa cacgctcggc gagtgcgagg ctttcggcga
      961 gcttgcgccg ctcggcaaga ttccgcgcca ggccagcatc atcaccaccg aggattcgac
     1021 cttcggcatt atctcccgcc aggtgttcat ggatacgctg ctgaccaagc cgaccgtcag
     1081 catgcagatc atcgacctgc tggtgcaccg catacaggac ctgaccgagg aagtcagcag
     1141 cctggcgctg gaagacgtct acaaccgcgt ggtacgcgta ctctacaagc acgccgagga
     1201 agtcggcgag aaactggtta ccgacaaact gacccagcag gatatcgcca gtcgcgtcgg
     1261 cgcgacccgc gaaatggtgc accgcatcct caaggagttg aaaaccggtg gctacatctc
     1321 catcgaaggc aagcacatca ccatcgagaa aaaactgccc cccggctggt gagcggcggt
     1381 gccggaaatg ctaacatagc gcttttttca gcgagggcct gaaacttggc tggtcatagc
     1441 aagtgggcga acatccagca ccgcaagaaa gcgcaggacg cgcgccgcgg caagttgttc
     1501 acgcgcctga ttcgtgaaat catggtcgcc gccagggccg gcggctccga tctcgatgcc
     1561 aacccgtcgc tgcgcaccgc ggtcgacaag gcgaaggcgc agtcggtgcc caaggacgcc
     1621 atcgagcgtg cgatcaaacg cggcgccggc gaactcgagg gttccgatta cgaagaaatc
     1681 cgttacgagg gttacgggcc cgctggtgtg gctttcatcg tcgactgcct ttccgacaat
     1741 cgcaatcgta ccgtcgccga ggtgcgccac gcgttcagca agggcggcgg caatctcggg
     1801 caatcgggtt cggtggccta cctgttcgac aatgtcggcg tgctgctgta ctcgcccgaa
     1861 cacgatgagg acgcggtcat ggaagccgcg ctcgaggcag gcgccgaaga cgtcatcgtc
     1921 gccgacgacg gcagcatcga gctgttgacg gcgcccgagc agtacatcgc ggtcaaacag
     1981 gcgatgatcg aagcgggttt cgagcccgag gaatccgatc tgacgctgcg cgcgcagacc
     2041 accaccgagc tggagctgga ggatgcgcaa aaggtattgc ggctggtcga cctgctggaa
     2101 ggttccgatg atgtgcagga agtctattac aacgccgata ttcccgaggc cgcctaccag
     2161 gacgaggcct gaatgatcat cctgggcctg gaccccggat cgcgcattac cggcttcggc
     2221 ctgatcgaca accagaccga ccgcatcgaa tatgtccaca gcggctgtat ccgtgtcagc
     2281 ggcgacagcc tggcagcgcg attgggacat atttacgctg aaatcgatgc cgtgatacgg
     2341 caatatcgcc cgcggcagat gggcatcgag cgcgtcttca tggcgcgcaa cccggattcg
     2401 gcgctgaagc tcggccaggc gcgcggcgcg gcgatctgcg ccgcgcacca ggcgggcctg
     2461 gagg
//
LOCUS       DATCZV010000503         4010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_464661, whole genome shotgun sequence.
ACCESSION   DATCZV010000503 DATCZV010000000
VERSION     DATCZV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464661"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VIW27_11360"
     CDS             complement(<1..439)
                     /locus_tag="VIW27_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HEY5740317.1"
                     /translation="MNLEPLLADLDDHGLGYWAARLRAGAGDWLVGHGDYPRWSAALE
                     SLPELKSVEARFDLPAVTVSADCADPRRLRQALRGLMPWRKGPFRIAGVDIDSEWRSD
                     LKWQRVEPALEPLTGRRVLDVGCGNAYYGWRMLAQSPRLVVGIE"
     gene            complement(436..1167)
                     /gene="cmoA"
                     /locus_tag="VIW27_11365"
     CDS             complement(436..1167)
                     /gene="cmoA"
                     /locus_tag="VIW27_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEY5740318.1"
                     /translation="MSDEAGRDQIFSRQLADVKAFEFNDEVASVFHDMISRSVPGYSL
                     LLRMIGLYAGIFVTPGSRVYDLGCSLGAASLIVAEQTRGLDCRIVAVDNSPAMIARCR
                     LHGSHEPPIDWRCEDIRQTSLQPASMVILNLTLQFLDPEERPALLQSIYRALDSGGML
                     VLSEKVVFDDPLENTRMTELYQGYKKTMGYSDLEISQKRNALENVLVPDSEQRHVERL
                     RNAGFAEIYCCFRAFNFVSWVAIKP"
     gene            complement(1164..2390)
                     /gene="tilS"
                     /locus_tag="VIW27_11370"
     CDS             complement(1164..2390)
                     /gene="tilS"
                     /locus_tag="VIW27_11370"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY5740319.1"
                     /translation="MNPTQASLDSIPDDTRRVFVAYSGGLDSSVLLHLLLDEPRRYEI
                     LPWHVNHGLLEVAGQMEDFCAREAHRLGLELRIDRLQLSALDANVEAEARRQRYRLFR
                     QQAGAGDCVVTAHHADDQAETFLLNALRGSGVAGLRGIAHRRPLGEGLLIRPLLAFSR
                     EQLEAYAAEHEIAWFNDPSNQSLRYDRNFLRREIVPRLKGRWPGLVQALSTSSEIQSE
                     TQLLLDEVAALDYRAVATESFGGISTLDLPGLLAFSEPRRKNLLRHWATAAGFTAMPH
                     ARLEELLQQLHARDDAQPEICMPGYAVRVYDQRLFLIADGEQPAIGGEFDFGQQQIIE
                     IEPLSLRLERRHIFQRLGVDDRQQSLSLKFRRCGERNRDRHRLKRLFQQHRVPPWQRD
                     RVAQVYLDGSLEGLLT"
     gene            complement(2426..3385)
                     /locus_tag="VIW27_11375"
     CDS             complement(2426..3385)
                     /locus_tag="VIW27_11375"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY5740320.1"
                     /translation="MDPTFLEFEQPIAELQAKILELRYLTDDSDINISQEISTLERKA
                     ESLTTSIFSKLTAWQISQLSRHPNRPYTLDYIDRMFSDFEFLHGDRCFADDAPIVGGI
                     GRLDDRPVMVIGQQKGRDTREKIKRNFGMPRPEGYRKALRLMKLAEKFKMPLITLVDT
                     PGAYPGIGAEERGQSEAIARNLFALAELKTPIVCTVIGEGGSGGALAICVGDRINMLQ
                     YATYSVISPEGCASILWKSADKASQAAEAMGITAARLHSLKLIDNVIKEPLGGAHRDY
                     DQIAETLRRNLVESLDALDAIPIDKLLDQRYKKLMSFGQYRET"
     gene            complement(3457..>4010)
                     /gene="pyrF"
                     /locus_tag="VIW27_11380"
     CDS             complement(3457..>4010)
                     /gene="pyrF"
                     /locus_tag="VIW27_11380"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY5740321.1"
                     /translation="EELVGLGFDVFLDLKYHDIPNTVAGAAAAAAKLGVWMLNVHALG
                     GKAMMRAAREGIEAAARRPLLIAVTVLTSLAPADLQDLGISTPLPRLVERLACDAIDC
                     GLDGVVCSAQEATALRAKLGDGPLLVTPGIRPEWAASDDQRRIATPRRAIDDGASYLV
                     IGRPITRHENPSEALRLINESLG"
BASE COUNT          725 a         1288 c         1272 g          725 t
ORIGIN      
        1 gttcgatgcc aaccaccagc cgcggcgact gggcgagcat gcgccagccg tagtaggcgt
       61 tgccgcagcc gacatcgagc actcggcggc cagtgagcgg ttcgagcgcc ggttcgaccc
      121 gctgccactt gagatcggat cgccactcgc tgtcgatatc gacgccggcg attcgaaacg
      181 gccccttgcg ccatggcatc aggccccgca gagcctgccg cagtcgccgc gggtcggcgc
      241 agtcggcact cacggtcacc gcgggcaggt cgaagcgcgc ctcgacggac ttaagttcgg
      301 gcaacgattc cagcgccgct gaccagcgcg gatagtcgcc gtgaccgacc agccagtcgc
      361 cggcgcccgc gcgcagtcga gcggcccagt acccgaggcc gtggtcgtcg aggtcggcca
      421 gcagcggttc caggttcacg gcttgatcgc cacccaggaa acgaagttga aagccctgaa
      481 acagcaatag atttccgcga agccggcatt gcgcaggcgt tcgacatgcc gctgctcgga
      541 gtcgggcacc agcacgtttt ccagcgcgtt gcgcttctgg ctgatctcga ggtcgctgta
      601 gcccatggtt ttcttgtatc cctggtagag ttcggtcatg cgcgtattct cgagcgggtc
      661 gtcgaatacc accttttccg acaacaccag cataccgccg ctgtcgagcg cccggtagat
      721 gctttgcagc agcgcgggtc tttcctcagg gtcgaggaac tgcagcgtca ggttgaggat
      781 taccatcgat gccggctgca gcgaagtttg ccgaatatcc tcgcagcgcc agtcgatcgg
      841 cggctcatga ctcccgtgaa gccggcacct ggcgatcatc gccggcgaat tgtcgaccgc
      901 gacgatgcgg caatcgaggc cgcgagtctg ctcggcgaca atcaggctcg cggcgcccag
      961 cgaacagccg aggtcgtaga cccggctgcc cggggtcacg aagatacccg cgtagagacc
     1021 gatcatgcgc aacaacagcg aatagccggg caccgagcgc gaaatcatgt cgtgaaaaac
     1081 gctggccacc tcgtcgttga actcgaacgc cttgacgtcg gccagttggc gggaaaatat
     1141 ttgatcgcgg cccgcttcgt cgctcatgtc agcaaaccct cgaggctgcc gtcaaggtag
     1201 acctgggcaa cccggtcacg ctgccacggc ggcacgcgat gctgttggaa caggcgcttg
     1261 agccggtggc ggtcgcggtt gcgttccccg catcgccgga acttcaggct cagcgactgc
     1321 tggcggtcgt cgacaccgag ccgttggaag atgtgacgtc gttcgaggcg cagtgaaagc
     1381 ggctcgattt caatgatttg ctgctggccg aagtcgaatt cgccgccgat tgccggctgt
     1441 tcgccatcgg cgatcaggaa caggcgctga tcgtaaaccc gtaccgcgta acccggcatg
     1501 cagatttccg gctgggcatc gtcgcgcgca tgcaattgct gcagcaattc ctcgagacgc
     1561 gcatgcggca tcgccgtgaa gccggctgcc gtcgcccagt gccgcagcag gttcttgcgt
     1621 cgcggctcgg aaaacgccaa caaaccgggc aagtccagtg tcgagatgcc gccgaagctt
     1681 tccgtggcca ccgcgcggta atcgagggcc gctacctcgt cgagcagcag ctgggtctcg
     1741 gactggattt cactgctggt cgacagcgcc tgtaccaacc cgggccagcg gcccttgagc
     1801 cgcggcacga tttcgcggcg caggaaattg cggtcgtaac gcagtgactg attgctggga
     1861 tcgttgaacc aggcgatttc atgctccgcc gcataagctt cgagctgctc gcggctgaac
     1921 gccagcaacg gccgaatcag caggccttca cccagtgggc gcctgtgagc gatgccgcgc
     1981 aatcccgcaa ccccagagcc gcgcagcgcg ttcaacagga aggtttcggc ctggtcgtcg
     2041 gcatggtgcg cggtaacaac gcagtcgccg gcgccggcct gttgtcgaaa cagccggtag
     2101 cgctggcggc gcgcctccgc ttcgacgttg gcgtcgagcg ccgacagctg caggcggtcg
     2161 attcgcaact ccagccccag gcggtgcgct tcgcgagcgc aaaaatcctc catttgcccg
     2221 gcgacctcga gcagcccatg attgacgtgc cagggcagaa tttcatagcg gcgcggttcg
     2281 tcgagcagca ggtgcagcag cacgctggaa tcgagaccgc cgctataggc gacgaatacc
     2341 cgccgcgtgt catcgggaat cgagtcgagg ctggcttgcg tcggattcat gcaatctgcg
     2401 gtcgcgccgc gcggttcgcc cgggatcagg tttcgcggta ctggccgaac gacatcagtt
     2461 tcttgtagcg ctggtcgagt aatttgtcga tcgggatcgc gtccagggcg tcgaggctct
     2521 ccaccaggtt gcgcctgagg gtctcggcta tctggtcgta atcgcggtgc gcgccgccga
     2581 gcggctcctt gatgacgtta tcgatgagct tcagcgagtg caggcgcgcg gcggtaatgc
     2641 ccatcgcctc ggctgcctgc gaggccttgt cggcgctctt ccacaggatc gacgcgcagc
     2701 cttccgggga gatcaccgaa taggtcgcgt actgcagcat gttgatgcga tcgccgacgc
     2761 aaatcgccag cgcaccgccg gaaccaccct cgccgatgac cgtgcagaca atcggcgttt
     2821 tcaactccgc caatgcgaac aggttgcgcg cgatcgcttc gctctgcccc cgctcctcgg
     2881 cgccgatgcc gggataggcg ccgggcgtgt cgaccagcgt gatcagcggc atcttgaact
     2941 tttcggccag cttcatcagc cgcagcgcct tgcgataacc ctcggggcgc ggcatgccga
     3001 aattgcgctt gatcttttcg cgcgtatcgc ggcccttctg ctggccgatc accatcaccg
     3061 ggcggtcgtc gagccgaccg atgccgccga cgatcggcgc atcgtcggcg aagcagcggt
     3121 cgccgtgcag gaattcgaaa tcgctgaaca tgcgatcgat gtaatccagc gtataaggcc
     3181 ggttcggatg gcgcgacaat tgcgatattt gccaggccgt caatttcgaa aaaatcgacg
     3241 tggtcagcga ttccgccttg cgttccaggg tactgatttc ctggctgata ttgatatcgg
     3301 agtcgtcggt gagatagcgc agctccaaga tcttggcttg cagctcggcg atcggctgtt
     3361 cgaattctaa aaaagtggga tccatgtgag aaatatcggc ctcgaaaccg gcgtaagttt
     3421 aaccattagc cccgggactg tcatccgcgc ggcgcgtcac ccgagcgact cgttgatcag
     3481 cctcagggcc tcggagggat tctcgtggcg ggtgatcggg cggccgatga ccagataact
     3541 ggcgccgtcg tcgatagccc tgcgaggcgt cgcgatacgc cgctggtcgt cgctcgcggc
     3601 ccactcgggc ctgatacccg gggtgaccag caggggtcca tcgccgagct tcgcacgcag
     3661 cgccgtggct tcctgagccg agcaaaccac gccgtcgagc ccgcagtcga tcgcgtcgca
     3721 cgccagccgc tctaccagcc gcggcaaggg cgtcgaaata cccagatcct gcagatcggc
     3781 cggcgccagg ctggtcagca ccgtgaccgc gatcagcagc gggcgcctcg ctgcagcctc
     3841 aataccttcg cgcgcggcgc gcatcatcgc cttgccaccg agcgcgtgga cattgagcat
     3901 ccagacgccg agcttggccg ccgcggcagc agcaccggca acggtattcg gtatatcgtg
     3961 gtacttgaga tcgaggaaca catcgaatcc gagcccgacc agctcctcga
//
LOCUS       DATCZV010000504         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_305619, whole genome shotgun sequence.
ACCESSION   DATCZV010000504 DATCZV010000000
VERSION     DATCZV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305619"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1307
                     /gene="metK"
                     /locus_tag="VIW27_11385"
     CDS             150..1307
                     /gene="metK"
                     /locus_tag="VIW27_11385"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY5740322.1"
                     /translation="MSDFLFTSESVSEGHPDKVSDQISDAVLDAILAKDPRARVAAET
                     LVKDNLVVLAGEITTTADVKYQDVVRQTIRRIGYTDPSIGFDAETCTILTVYGQQSPD
                     IAQGVNEDQGLDHEQGAGDQGLMFGYACDETPSLMPLPIYLAHRLVERQSETRRDGRL
                     PWLRPDAKSQVTVRYVDGRPDAIDTVLISTQHAPGIKHEQLAEGVIEEIIKPVLPKNL
                     VKGDLRYLVNPTGAFEIGGPKGDCGLTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLARKCLVQVSYAIGVARPTSVMVTTEGTGKLSDDKLSKLVLE
                     HFDLRPKGIVTMLDLLRPIYEKTAAYGHFGREDPEFTWEATDRALALKEAA"
     regulatory      1325..1395
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1464..>2121
                     /locus_tag="VIW27_11390"
     CDS             1464..>2121
                     /locus_tag="VIW27_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5740323.1"
                     /translation="MNAPNPKFTDYKVADLALADWGRKEMTIAEHEMPALMAMREEFA
                     KSKPLQGARIAGSLHMTIQTAVLIETLSALGAEVRWASCNIFSTQDHAAAAIAAGGTP
                     VFAYKGESLDEYWEFTHRIFEFGSGKGPNMILDDGGDATLLMHLGVKAEKDPSVLAKP
                     TSEEETALFAAIKKRLAAQPNWYSKIAKEIKGVTEETTTGVHRLYQMAKDGQLMFPAI
                     N"
BASE COUNT          429 a          679 c          665 g          348 t
ORIGIN      
        1 agcggcagca ccgcgattgc gcgcatcagc acgttgagca tgcgtttggc gaggcggagc
       61 gatgcggcaa tctttggtat ccttgcgccc ttctcgcaac tgcgccgggc cttcagcgtg
      121 gcgcatcttt caaacgaggc gcacacgcaa tgagcgattt cctattcacg tcggagtcgg
      181 tgtcggaagg gcatcccgac aaggtttccg accagatttc ggacgcagta ctcgacgcaa
      241 ttctagccaa agacccgcgc gcccgcgtcg cggcggaaac cctggtcaag gacaacctgg
      301 tcgtgctcgc cggcgagatc acgacgaccg cggacgtgaa atatcaggac gtcgtgcgcc
      361 aaaccatccg gcgcatcggt tacaccgacc catcgatcgg cttcgatgcc gaaacctgca
      421 ccatccttac cgtctacggc cagcaatcgc cggacatcgc gcagggcgtg aacgaggacc
      481 agggcctcga tcatgagcag ggcgccggcg accagggact gatgttcggt tatgcctgcg
      541 acgagacgcc gtcgctcatg ccgctgccga tctatcttgc gcaccgactc gtcgagcgcc
      601 aatcggagac acgacgcgac ggccgtctgc cctggctgcg cccggacgcg aagtcgcagg
      661 tcacggtgcg ctacgtcgac ggcaggccgg acgcgatcga caccgtgctc atttcgacgc
      721 agcacgcacc gggtatcaag cacgagcaac tggcggaggg tgtcatcgag gagatcatca
      781 agccggtcct gccgaagaac ctggtcaagg gtgacctgcg ctatctggtg aatcccaccg
      841 gcgcgttcga gatcggcgga ccgaagggcg actgcggcct caccggccgc aagatcatcg
      901 tcgacaccta tggtggctcg gcgccgcacg gcggcggcgc attctccggc aaggatccgt
      961 cgaaggtgga ccgctcggcc gcctacgcgg ggcgctacgt ggcaaaaaac atcgtcgccg
     1021 cgggcctggc gaggaaatgt ctggtgcagg tgtcctacgc gatcggcgtt gcgcggccga
     1081 cctcggtgat ggtgacgacc gaaggcaccg gcaagctgtc ggatgacaag ctttcgaaac
     1141 tggttctcga gcacttcgac ctgcggccca aggggatcgt gacgatgctc gatttgctgc
     1201 gaccgattta cgaaaaaaca gcggcttacg ggcatttcgg gcgcgaggac cccgagttca
     1261 cctgggaagc cacggatcgc gcactcgccc tgaaagaggc cgcctgaccg ggtataattt
     1321 cgatcctgct gaggagcgct gcatcccctg tgcaccgggg gtcaggctca gcgaaatcaa
     1381 cggcgctcgc atcgaatccg gtgcgggcgc tttgtttttg gggctccccc cggacagtca
     1441 ttattcgata ggagcttctc gccatgaacg cacccaaccc gaagttcacc gactacaagg
     1501 tcgccgacct cgcgctcgct gactggggcc gcaaggaaat gaccatcgcc gagcacgaga
     1561 tgccggcgct gatggcgatg cgtgaggaat tcgccaagtc caagcccctc cagggcgcgc
     1621 gcatcgccgg ctcgttgcac atgacgatcc agaccgccgt attgatcgag acgctgagcg
     1681 cgctcggtgc cgaagtgcgc tgggcctcgt gcaatatctt ctcgacccag gaccacgcag
     1741 cagcggcgat cgccgctggc ggcacccccg tgttcgccta caagggcgaa tcgctcgacg
     1801 agtactggga gtttacccac cgcatcttcg agttcggcag cggcaagggc cccaacatga
     1861 tcctcgatga cggcggcgac gccacgctgc tcatgcacct tggcgtcaag gcggaaaagg
     1921 atccttcggt actcgccaag ccgacgagcg aggaagaaac cgcgctgttc gcggcgatca
     1981 agaagcgcct cgccgcacag ccgaactggt actcgaaaat cgcgaaggaa atcaagggcg
     2041 tcaccgagga gacgaccacc ggcgtacacc gcctgtacca gatggcgaaa gatggccagc
     2101 tgatgttccc cgcgatcaac g
//
LOCUS       DATCZV010000505         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_253619, whole genome shotgun sequence.
ACCESSION   DATCZV010000505 DATCZV010000000
VERSION     DATCZV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253619"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VIW27_11395"
     CDS             complement(<1..356)
                     /locus_tag="VIW27_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740324.1"
                     /translation="MATLAACTLYQPLPWSRAGAAVLPEERADLLYHRYDGGGAEIDG
                     PSLLVRKNVGDAVSVGFNHYVDNVTSASIDVLVSASEYTERREENSLNLDYLRQKTTM
                     SLGYTRSLESDFDATTL"
     gene            complement(413..640)
                     /locus_tag="VIW27_11400"
     CDS             complement(413..640)
                     /locus_tag="VIW27_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEY5740325.1"
                     /translation="MKRITAAAVVMLAGLQLAGCAVDPWVKPYERARLADPIMSFNRD
                     PVSASYLHHVYDSREGARGAGIATGGGCGCN"
     gene            complement(645..1142)
                     /locus_tag="VIW27_11405"
     CDS             complement(645..1142)
                     /locus_tag="VIW27_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5740326.1"
                     /translation="MKCLLSRILPGLLLACVLGSAGAAPVSGPAPDFTLKSLSGNNMK
                     LSEMAGNVVLINFWASWCGPCREEMPLLNALHNKYQPLGFTVLGINVEEQVDSARGFL
                     RDFPVDFPVLLDHKNQVSQLYEVVAMPTTVVVDRDGQLRYLHQGYKSGDEEKYRQMVK
                     KLVRE"
     gene            complement(1153..1770)
                     /locus_tag="VIW27_11410"
     CDS             complement(1153..1770)
                     /locus_tag="VIW27_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY5740327.1"
                     /translation="MVAVLAMPVGEAAAQASDGEETVLVEPQIERSEFDESMIESDDF
                     EIALYTGYLAVEDFNTDFVTGIKLGYHVTEDFFVQASYGFGSVGETSFERLSGGAPLL
                     SDAERDIEYYLVALGFNLFPGEAFVTDSTTFNTVLYISGGVGSTTFAGDDRFTIAYAV
                     GHRTLFADGFSLDIEMRDLIFEQDIFGSEETTNNLEFSVSLNLFF"
     gene            complement(1773..>2083)
                     /locus_tag="VIW27_11415"
     CDS             complement(1773..>2083)
                     /locus_tag="VIW27_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740328.1"
                     /translation="GRSSSSTLVKANLVMQPLPEFKYSPYMTLGMGRIKVDPNATLIS
                     PVEDTNTLAQFGLGIQRYISRSFLARFEFNEYIIFAADSTRNDNEEVNEWKFGFAVFF
                     "
BASE COUNT          423 a          629 c          626 g          405 t
ORIGIN      
        1 agggtggtgg cgtcgaaatc gctttcgaga ctgcgggtgt agccgagact catggtcgtc
       61 ttctgccgca ggtagtcgag gttgagcgaa ttttcctcgc ggcgctcggt gtactcgctg
      121 gcgctgacca gtacgtcgat cgacgcgctg gtgacattgt cgacgtagtg gttgaagccc
      181 accgataccg cgtcaccgac attcttgcgc accagcagtg aaggtccgtc gatctccgcg
      241 ccgccgccat cgtagcgatg gtatagcaaa tcggcgcgtt cctcgggcag gacggcggcg
      301 ccggctctcg accatggcaa cggctggtag agcgtgcagg cggccagcgt ggccagcgcg
      361 atcgatcgca ggtgcgggat atcgagcagc ggcacacgca cggcaacgaa aatcagttgc
      421 aaccgcaacc gccgccggtc gcgatgccgg caccgcgagc accctcgcgc gaatcataga
      481 cgtggtgcag gtaactcgcc gataccgggt cacggttgaa gctcatgatc ggatcggcca
      541 gccgggcgcg ctcgtagggc ttgacccagg gatctaccgc gcagcccgca agctgcagcc
      601 cggcgagcat gacgacggct gcagcggtga ttcgcttcat gcgcctattc ccgcaccagt
      661 ttcttgacca tctggcgata tttctcctcg tcgccggact tgtatccctg gtgcaggtag
      721 cgcagctggc cgtcgcggtc gacgaccacc gtggtcggca tggcgaccac ctcgtacagc
      781 tggctgacct ggttcttatg gtcgagcaac accgggaagt cgaccgggaa gtcccgcaga
      841 aagccgcgcg cgctgtcgac ctgttcctcg acattgatac cgagcacggt aaagccgagc
      901 ggctggtact tgttgtgcag ggcgttgagc aacggcattt cctcgcggca gggaccgcac
      961 caggacgccc agaaattgat cagcaccacg ttgccggcca tttcgctcag cttcatgttg
     1021 ttgccgctca gcgacttgag cgtaaaatcc ggagccgggc cggaaaccgg ggccgcgccg
     1081 gcgctgccca gcacgcaggc cagcaacaac ccgggcagaa ttcgcgacag taaacatttc
     1141 attccatttc tcctagaaaa acaggttcag tgatacgctg aattcgagat tattggtggt
     1201 ctcctcgcta ccgaatatat cctgctcgaa aatcaggtcc cgcatctcga tgtccaggct
     1261 gaagccatcc gcgaacagtg tgcgatggcc gactgcatag gcgatggtga aacgatcgtc
     1321 gccggcaaag gtcgtgctgc cgaccccgcc cgaaatatac agcacggtat tgaatgtagt
     1381 cgaatcggtg acaaatgcct cgccgggaaa caggttgaag ccgagcgcga ccaggtagta
     1441 ttcgatatcg cgctccgcgt ccgacaacag cggtgcgccg ccgctgaggc gctcgaagct
     1501 ggtttcgccg acactgccga aaccgtagga ggcctgcacg aagaaatcct cggtgacatg
     1561 gtagccgagc tttatcccgg tcacgaaatc ggtattgaaa tcctccacgg cgagatagcc
     1621 ggtgtagagc gcgatctcga aatcgtcgga ttcgatcatc gattcgtcga actcgctgcg
     1681 ctcgatctgc ggctcgacca ggaccgtctc ttcaccatcg ctggcctgcg ccgcggcctc
     1741 gccgaccggc atcgccagca cggcgaccat aactagaaaa ataccgcgaa accgaatttc
     1801 cactcgttta cttcctcgtt gtcgttgcgg gtgctatcag cggcgaaaat gatgtactcg
     1861 ttgaattcga agcgcgccag gaaactgcgg ctgatatagc gttgtatgcc gagcccgaac
     1921 tgggcaaggg tattggtatc ctcgaccggt gagatcagcg tcgcgttcgg atcgaccttg
     1981 atcctgccca tccccagcgt catgtacggg gaatacttga attccggcag gggttgcata
     2041 accaggttcg ctttcaccag cgtactgctg gaactgcggc cca
//
LOCUS       DATCZV010000506         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_42194, whole genome shotgun sequence.
ACCESSION   DATCZV010000506 DATCZV010000000
VERSION     DATCZV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42194"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VIW27_11420"
     CDS             complement(<1..798)
                     /locus_tag="VIW27_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740329.1"
                     /translation="MEQSADIAAIVEAALADGLPSADRLRREARDLASRTCLGRSAFL
                     RAQGCASELEYKRAAMRDGRIMYHAHVGMNDIGATAAALVEIHRQLQARGQHLDRAGF
                     ALDRRMGLPPELQAAAAAETGPMLDRQADWAALAEAAPIQPHLGDFMIGQPASVANTL
                     HALAIGCTTIGNLSQYFTFEAPGWSDAGKTAAQTCEAMMLLAEFREQGVMLHSYLEDG
                     YGALFRQCGSVAAWAMLERYIVEELIGARLSHCIGGLTRDPVKRAGWV"
     gene            complement(799..1176)
                     /locus_tag="VIW27_11425"
     CDS             complement(799..1176)
                     /locus_tag="VIW27_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5740330.1"
                     /translation="MKQHEKLDYVEFPAARPERTREFFRQAFGWSFEEFGPDYCAFSD
                     QGLDGGFYRSELYSTTRSGAALLVFYSEDLEATLARIEAAGGRIEKPIFAFPGGRRFH
                     FCEPSGNEFAVWSDQPAPRPRGD"
     gene            complement(1173..>2132)
                     /locus_tag="VIW27_11430"
     CDS             complement(1173..>2132)
                     /locus_tag="VIW27_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5740331.1"
                     /translation="HDLQGGPPVTALLVQNTNPVVVAPDTRRVLQGFARDDLFVCVHE
                     QFMTETAELADIVLPATMFLEHDDFYRGGGHTYLQTTRALVTPYAECRSNHWVLGQLA
                     SRLGFTHPGFELGERELIDHCLRQSGFGGEQALYDNHWVDCARGFEDDNFLDGFATPD
                     GRFHFQPDWSRVGEDCTAMPVLPDHMPVANDVDDAHPFRLVAAPARQFLNTTFTETRS
                     SRRAERRPTLLIRGDDAASLGIADGELVRIGNRLANIRLHAKLFDGMQRGVVIVESIW
                     PNGSFVDGLGVNALVSSEPGKPNGGATYHDTAVWIETASAEES"
BASE COUNT          351 a          699 c          726 g          356 t
ORIGIN      
        1 gacccagccg gcgcgcttga ccggatcgcg ggtcaggccg ccgatgcagt gtgacagtcg
       61 cgcgccgatc agctcttcga cgatgtaacg ctcgagcatc gcccaggcgg cgacgctgcc
      121 gcattgccgg aacagcgcgc cgtagccatc ctcgagatag gagtgcagca tcaccccctg
      181 ttctctgaac tccgccagca gcatcatcgc ctcgcaggtc tgcgccgcgg tcttgccggc
      241 gtcactccag cccggggcct cgaaggtgaa atactgcgac aggttgccga tcgtcgtaca
      301 gccgatggcg agcgcgtgca gcgtgttggc caccgaggcc ggctggccga tcatgaaatc
      361 gccgaggtgc ggctgaatcg gtgccgcctc ggcgagcgct gcccagtcgg cctgccgatc
      421 cagcatcggt ccggtttccg cggcggcggc ggcctgcaac tcgggcggca ggcccatgcg
      481 gcgatcgagc gcgaagccgg cgcggtccag gtgctggccg cgcgcctgca gctggcgatg
      541 gatttcgacc agcgccgccg cggtggcgcc gatatcgttc atgccgacgt gagcgtggta
      601 catgatgcgc ccgtcgcgca tcgcggcgcg cttgtactcg agttccgacg cgcagccctg
      661 cgcgcgcagg aatgccgagc gaccaaggca ggttcggctg gcgagatcgc gcgcctcgcg
      721 gcgcaggcga tcggcgctgg gcaagccgtc cgccagcgcc gcttcgacga tcgcggcgat
      781 atccgccgat tgctccattc agtcgcctcg aggccgcggg gccggctgat ccgaccacac
      841 cgcgaactcg ttgccgctgg gttcgcagaa atgaaagcgg cggccgccgg ggaatgcgaa
      901 tatcggtttc tcgatgcgcc cgccggcggc ctcgatgcgc gccagcgtgg cttccaggtc
      961 ttcgctgtag aacaccagta gcgcggcgcc gctgcgggtt gtcgagtata gctccgagcg
     1021 ataaaagcca ccgtccagcc cctggtccga aaacgcgcaa tagtcggggc cgaattcctc
     1081 gaagctccag ccgaaagcct ggcgaaagaa ttcgcgcgtg cgttccggcc tggcggcggg
     1141 aaattcgacg taatccagtt tttcgtgctg tttcatgact cctctgcgct ggcggtttcg
     1201 atccacaccg cggtgtcgtg gtaggtcgcg ccgccattcg gcttgccggg ctcggacgat
     1261 accagcgcgt tgacgccgag cccgtcgacg aatgagccgt tcggccagat gctctcgacg
     1321 atgaccacgc cgcgttgcat gccatcgaac aatttcgcgt gcagccgaat gttggcgagg
     1381 cggttgccga tacgcaccag ttcgccgtcg gcgatgccga ggctggcggc gtcgtcgccg
     1441 cgaatcaaca gcgtcggtcg gcgctcggca cgccgcgagc tgcgcgtctc ggtgaaggtg
     1501 gtattcagaa actggcgcgc cggcgccgcg accagccgaa acggatgcgc gtcgtcgacg
     1561 tcgttggcga ccggcatgtg atcgggcagc accggcatcg cggtgcaatc ctcgccgaca
     1621 cggctccagt cgggctggaa gtgaaagcgg ccatcgggag ttgcgaaacc gtcgagaaaa
     1681 ttgtcgtcct cgaagccgcg cgcgcaatcg acccagtggt tgtcgtagag ggcctgctcg
     1741 ccgccgaaac cgctttgcct gaggcaatgg tcgatgagtt cgcgttcgcc aagttcgaag
     1801 ccggggtggg taaagccgag ccgactcgcc agttgcccga gcacccagtg gttgctgcgg
     1861 cactcggcgt agggcgtcac cagcgcgcgc gtggtctgca ggtaagtatg gccgccgccc
     1921 cgatagaaat cgtcgtgctc gagaaacatc gtcgccggca gcacgatgtc ggcgagctcg
     1981 gcggtctcgg tcatgaattg ctcgtgcacg caaacgaaga ggtcgtcgcg cgcgaacccc
     2041 tgcagtaccc ggcgggtatc gggggcgacc accaccggat tggtgttctg caccagcagc
     2101 gcggtcaccg gcggaccacc ctgcagatca tg
//
LOCUS       DATCZV010000507         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_783192, whole genome shotgun sequence.
ACCESSION   DATCZV010000507 DATCZV010000000
VERSION     DATCZV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783192"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VIW27_11435"
     CDS             <1..1035
                     /locus_tag="VIW27_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5740332.1"
                     /translation="VGLAEPVDEDLLSKWETLVYDSCTMCGRCSMICPVGNDITGLIR
                     KTREGMAAAGHAPEGLIGATRRAITIGSPMGVKLPALQAQIRHAEEELGLKVPVDVEG
                     ADYMLLLSSMEIMNFPEFIGAVTKIFDKAGVSWTISSQAFEATNSGIQIGVSDLAAEL
                     VQRVVDGAEKLKVKTVISPECGHAYFALRWDGPNLVGKAFGFKVRHILEVLDELRREG
                     RLKTTGFEEQRVTYHDPCQIARRGGVIDEPRNLLKMVCKDFVEMEDSGKMNWCCGAGG
                     GVSSNERADELRLKVFQRKKSQLDDIRAEAMVSACSNCRIHLEDGLEEYNMDIPVLSL
                     TETLAEHLQD"
     gene            1263..2993
                     /locus_tag="VIW27_11440"
     CDS             1263..2993
                     /locus_tag="VIW27_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY5740333.1"
                     /translation="MSTSADRIVVDPITRIEGHLRIEAQMEGDRIASAYSSGTMVRGI
                     EIILRGRDPRDAWAFAQRICGVCTLVHGLASIRSVEDALDYEIPKNAELIRNLMNGAQ
                     YIHDHVMHFYHLHALDWVDVVSALDADPGETSALAQSLSSYPKSSPGYFADTKKKLKD
                     FVEAGQLGIFAKAYWGHPAYKLPPAANLMAVAHYLEALTWQRDVARLHTIFGGKNPHP
                     NFVVGGVPCAIDLNSDSAINTKRLTQVQDIITQMKTFVDQVYVPDTLAIASFYKDWGS
                     RGEGLGNFLTYGDFPSNGMGDPSGYLIPPGAILDRDLGTIHEVDMHAPEQIQEYVAHS
                     WYDYEAGKQQGLHPYDGETNLNYTGPKPPYQHLDIEQSYSWLKSPRWKGHAMEVGPLA
                     RVLMLYASGHQQTRELVGMTLNQLEIPVEALFSTLGRTAARTLETKILADAMQGWYDE
                     LIANIKAGDTRTFNERLWEPSSWPKQAQGVGYMEAPRGGLAHWIVIEDEKIKNYQAVV
                     PSTWNAGPRDASNQPGAYEAALQDNHTLHDPQQPVEILRTIHSFDPCIACAVHLTDPD
                     GKELIKVKVR"
     gene            2990..3157
                     /locus_tag="VIW27_11445"
     CDS             2990..3157
                     /locus_tag="VIW27_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740334.1"
                     /translation="MKKFLSLALLWSSSSLVLAHPGHGTMSLVDLWPLLTVGLLIAVV
                     GALALGRGKPD"
BASE COUNT          685 a         1092 c         1011 g          505 t
ORIGIN      
        1 gtcggcctcg ccgaaccggt cgacgaggat ttgctgagta aatgggaaac gctggtctat
       61 gacagctgca ccatgtgtgg ccgctgctcg atgatttgcc cggtcggcaa cgatatcacc
      121 ggcctgatcc gcaagacccg cgagggcatg gccgccgccg gccacgcccc cgaggggctg
      181 atcggcgcca cccggcgcgc gatcaccatc ggcagcccga tgggcgtcaa gctgccggcg
      241 ctgcaggcgc agatccgcca tgccgaggaa gaactcggcc tgaaggtgcc ggtcgacgtc
      301 gagggcgccg attacatgct gctgctgtcg tcgatggaaa tcatgaactt tcccgagttc
      361 atcggcgcgg taaccaagat attcgacaag gccggagtca gctggacgat ttcgtcgcag
      421 gccttcgagg ccaccaacag cggcatccag atcggcgtct ccgacctcgc cgccgaactg
      481 gtgcaacgcg tcgtcgacgg cgccgaaaag ctcaaggtca agaccgtcat cagccccgag
      541 tgcgggcacg cctacttcgc cctgcgctgg gacggcccca acctggtcgg caaggccttc
      601 ggcttcaagg tgcgccatat cctcgaagtg ctcgacgaac tgcgccgcga gggtcgcctg
      661 aaaaccaccg gcttcgagga gcagcgggtg acctaccacg atccgtgcca gatcgcccgc
      721 cgcggcggcg tcatcgacga gccacgcaac ctgctcaaga tggtctgcaa ggatttcgtc
      781 gagatggaag acagcggcaa gatgaactgg tgctgcggcg ccggcggcgg ggtcagctcc
      841 aacgagcgcg ccgacgaact gaggctcaaa gtgttccagc gcaagaagag ccagctcgac
      901 gatatccgtg ccgaggcgat ggtcagcgcc tgctcgaact gccgcatcca cctcgaggac
      961 ggactcgagg aatacaacat ggatattccg gtgctgagcc tgaccgaaac cctggcagag
     1021 catctgcaag actgacttgc cgtgagccga gacaaccatg tcctcgatcg acgacgacca
     1081 gtccagtcaa gccctgccag cgcccggcaa ccacgcgtgg caggcgacgc ggcctggccg
     1141 cagggccggg accggcatga cgatcctcga cgcgccgccg gcgccaaccg ccgagtcggc
     1201 aggagcgcgg caccgccggg ctctcgaaaa ccctgttaaa gcatgagccg gagacagcag
     1261 caatgagcac atccgcagat agaatcgtag tagacccgat tacccgcatc gaggggcatt
     1321 tgcgcatcga ggcgcaaatg gaaggcgacc gcatcgccag cgcctattcg tcgggcacca
     1381 tggtgcgcgg catcgagatc atactgcgcg ggcgcgaccc gcgcgacgcg tgggccttcg
     1441 cgcagcgtat ctgcggcgta tgcacactgg tccacggcct ggcgtcgatt cgctcggtcg
     1501 aagatgcgct cgactacgag atcccgaaga acgccgaact gatccgcaac ctgatgaacg
     1561 gcgcgcagta catccatgat cacgtgatgc acttttacca cctgcacgcg ctcgactggg
     1621 tcgacgtggt gtcggcgctc gacgccgacc cgggcgaaac ctcggcattg gcgcagtcgc
     1681 tgtccagcta ccccaagtcc tcccccggct acttcgccga caccaagaaa aagctcaagg
     1741 acttcgtcga ggccggccaa ctcggcatct tcgccaaggc atactgggga cacccggcgt
     1801 acaagctgcc accggcagcc aacctgatgg cggtagcgca ttatctcgag gcactgacct
     1861 ggcaacgcga cgtcgcacgc ctgcacacca tcttcggcgg caagaatccg catcccaatt
     1921 tcgtcgtcgg cggcgtgccc tgcgcgatcg acctgaactc ggactcggcg atcaacacca
     1981 agcgcctgac gcaggtacag gacatcatca cgcagatgaa aaccttcgtc gaccaggttt
     2041 atgtgccgga tacgctggcg atcgccagct tctacaagga ctggggcagc cgtggcgagg
     2101 gtctcggcaa cttcctgacc tacggcgact ttccatccaa cggcatgggc gacccgtcgg
     2161 gttacctgat cccgcccggc gcgatcctcg accgcgacct cggcaccatc cacgaggtcg
     2221 atatgcatgc gccggagcag atccaggagt acgtcgcgca ttcctggtac gactacgaag
     2281 ccggcaagca gcaggggctg cacccctacg acggcgaaac caacctcaat tacaccgggc
     2341 cgaaaccgcc ttaccagcac ctcgatatcg agcaatccta ctcgtggctg aaatcgccgc
     2401 gctggaaggg tcacgcgatg gaggtcgggc cgctggcacg cgtactgatg ctgtacgcca
     2461 gcgggcacca acagacccgc gaactcgtcg gcatgacgct caaccagctc gagattccgg
     2521 tcgaagccct gttctcgacg ctcggccgca ccgcggcgcg cacgctggag acaaagatcc
     2581 tggccgacgc gatgcagggc tggtacgacg agctgatcgc caacatcaag gccggcgata
     2641 cgcgcacttt caacgaaagg ctgtgggaac cgtcgagctg gccgaaacag gcgcagggcg
     2701 tcggctacat ggaagcgccg cgcggcggcc tcgcgcactg gatcgtcatc gaggacgaaa
     2761 agatcaagaa ttaccaggcg gtggtgccca gcacctggaa tgccggaccg cgcgacgcat
     2821 cgaaccagcc cggcgcctac gaggccgcgc tacaggataa ccataccctg cacgaccccc
     2881 agcaaccggt cgaaatcctg cgcacgatac acagcttcga tccctgcatc gcctgtgccg
     2941 ttcacctgac cgacccggac ggcaaggaac tgataaaggt aaaagtacga tgaagaaatt
     3001 cctgtcgctc gcgctgctgt ggtcgtcgag ctccctggtt ctcgcccatc ccgggcatgg
     3061 tacgatgtcg ctagtcgacc tgtggccgct gctgacagtc gggctgctga ttgctgtggt
     3121 cggcgcgctg gccctcggcc gcggcaagcc ggactgattc ctcgacgccg gccgccacca
     3181 taaaaaaagg cgccggtcta gctaaagcgc gaccggcgca acatgggggc aatccggtta
     3241 gccgacaggc gacggggtcc ggatcaccga gggcagctga atgggctatc agc
//
LOCUS       DATCZV010000508         4876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_729890, whole genome shotgun sequence.
ACCESSION   DATCZV010000508 DATCZV010000000
VERSION     DATCZV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729890"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1973)
                     /locus_tag="VIW27_11450"
     CDS             complement(75..1973)
                     /locus_tag="VIW27_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY5740335.1"
                     /translation="MLMLVAAVATPTVQASYEQQRRLFHDAAYALQRNQLSRFNLLLK
                     KLGDYPAKPYLEYDAFKLQVGRTSPQQAEMFLQRHADFPFAYHARGKWLNELARRGDW
                     ENYLAFFDGRENTRLKCLAFRARIKFGQLDNLDEEIARVWLRGYSQPSQCDPAFEYYL
                     GSQENPEQVIWLRIEKAFKARRPGLARYLGRKLDSASQDLVEIWYRAHVRPEQMLKKL
                     GDREDNERNRAIIAHAVDRLARRDSLKALENWNLVRDHFAFSPEQKRSIQLRIALSAA
                     YQHRPEARTLLSNLDSASMNDQAFLWLARIQLRGSDWPGLLDTINRMPQHLHRENEWQ
                     YWLSRSLEAEGKLAESLTLLEELSGRSSYYGFLAADRLNRDYRIEQEDAASVDVDEQA
                     FLEANPHMLRARELYFLDRLVDAKREWFQALRYLDKDQIKQAATLASRWQWHDSAIRT
                     VARAQHRRDYSLRFPMPYKQQVFASAQANELDPSLIYGVMRRESLFDPLARSKVGALG
                     LMQLMPSTARRVARSLGMKRPGQADILHVENNIHMGAHYLKTVMKRFDNNVALATAAY
                     NAGPGNVRRWLPEKDAMPADLWVETVPFGETRKYVQAVLAYSTVFDKSLGKNTLMSSR
                     MGDIKPRY"
     gene            complement(2092..3477)
                     /locus_tag="VIW27_11455"
     CDS             complement(2092..3477)
                     /locus_tag="VIW27_11455"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY5740336.1"
                     /translation="MGGKKIKATDSLGSVEIPSGALYRAQTQRALDNFRISGLRLPAA
                     MIRALALLKQAGARVNARLGELDAERAAAIDAAAAKILDGEHADQFGIDVFQTGSGTS
                     SNMNVNEVIATLASAEGLEIDANDHVNMGQSSNDVFPSAIHVAASLAVHEQLLVAIQN
                     LETAIAGRSEALDDTVKTGRTHLMDAMPLSLGQEISAWGAQLKNDRYRIQAALTRLQR
                     LAIGGTAVGTGVNTGSDFGDRVCALLRAETGIDFKPMDNRFEGIATQNSTLELSGQLR
                     VLATSLTKISNDLRWMNSGPLAGLGEICLEALQPGSSIMPGKVNPVIPEAVLMVAAQV
                     TGNDATIAVAAQSGNFQLNVMLPVIAYNLLQSIEILANSCNHLAPVIDRFSVNQAVLA
                     ENLARNPILVTALNPLIGYRRAAEIANRAMRENRPILDVAIEMTDIEESRLRHLLDPA
                     RLAHPHRDPGE"
     gene            complement(3910..>4876)
                     /gene="bioA"
                     /locus_tag="VIW27_11460"
     CDS             complement(3910..>4876)
                     /gene="bioA"
                     /locus_tag="VIW27_11460"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY5740337.1"
                     /translation="SVFLCDSGSVAVEVAMKMAIQYWNARGLPSKQRFAALRNGYHGD
                     TLGAMSVCDPVTGMHSLFNTMLTSQHFVARPASRFGESLRDSDAQALETVLRERGDEI
                     AALILEPIVQGAGGMYFYSAEFLACARELCDRYGVLLIADEIATGFGRSGKLFACEHA
                     GVAPDILCLGKALTGGYLSLAATLATTEISDTIDGGSPGVFMHGPTFMGNPLACAVAL
                     ESIELLLESDWQARVARIEKNLARWLAPCASLPQVAELRCLGAIGVVEMREPVNMAEI
                     TRQFVDAGVWVRPFGRLVYLMPAFVISDDELRQLCESVVTVVERQ"
BASE COUNT          928 a         1493 c         1496 g          959 t
ORIGIN      
        1 agatcagttt gagtttgaag cagtttccgg aatagatatc gccgtatatc ttcatcgggc
       61 cggcctcggg tcggtcagta tctcggcttg atatcaccca tgcggctgga catcaaggta
      121 tttttaccca gcgacttgtc gaataccgtc gagtaggcga gcaccgcctg cacgtatttg
      181 cgggtctcgc cgaacggcac ggtttcgacc cacagatccg ctggcatggc gtctttttcc
      241 ggcagccagc gcctgacgtt gcccggcccg gcattgtagg ccgcggtggc cagcgcgaca
      301 ttgttgtcga aacgcttcat taccgttttc aggtaatgcg cgcccatgtg aatattgttc
      361 tcgacatgca gaatgtccgc ctgcccggga cgcttcatgc ccagcgaacg cgccacccgc
      421 cgcgcggtgc tgggcattag ctgcatcagg cccagcgcgc cgaccttgga gcgcgccagc
      481 gggtcgaaca gactctcgcg ccgcattacc ccgtagatca gcgacggatc cagttcgttt
      541 gcctgcgccg atgcgaacac ctgctgcttg tagggcatcg gaaaacgcag gctgtagtcg
      601 cggcggtgct gcgcccgggc gacggtccga attgcgctgt cgtgccactg ccagcgcgag
      661 gccagcgtgg cggcctgctt gatctggtcc ttgtcgaggt agcgcagcgc ctggaaccac
      721 tcgcgcttgg catccaccag gcggtcgaga aaatagagtt cgcgcgcgcg cagcatgtga
      781 ggattggctt cgaggaaagc ctgctcatcg acatcgacgc tggcggcgtc ttcctgctcg
      841 atacgatagt cgcgattgag gcgatcggcg gcgagaaatc cgtagtagct gctcctgccg
      901 gacagttcct cgagcaacgt cagcgattcc gccagcttgc cctcggcttc gagcgagcgc
      961 gacagccagt attgccactc gttttcgcgg tgtaaatgct ggggcatacg attgatggta
     1021 tcgagcaaac ccggccagtc gctgccgcgc aattgtatgc gcgccagcca caggaatgcc
     1081 tgatcgttca tcgatgccga atcgaggttc gacaacagcg tgcgcgcctc cggccgatgc
     1141 tggtaagccg ccgacagcgc gattcgcaac tggatgctgc gcttttgctc ggggctgaaa
     1201 gcgaagtggt cgcgaaccag gttccagttt tccagcgcct tgaggctgtc gcgacgcgcc
     1261 agccggtcga cggcgtgcgc aatgatcgcg cggttgcgct cgttgtcttc gcggtcaccg
     1321 agtttcttga gcatttgctc ggggcgcaca tgcgcccggt accagatctc gaccagatcc
     1381 tgggatgccg aatccagctt gcggccgagg taacgggcaa gcccgggacg acgcgccttg
     1441 aaggcttttt cgattcgcag ccagatcacc tgttcgggat tttcctggct gccgaggtaa
     1501 tactcgaagg ccgggtcgca ctgcgagggt tgcgaataac cccttaacca gacgcgggcg
     1561 atttcctcgt cgagattgtc gagctgaccg aacttgatgc gcgcgcgaaa agcgaggcat
     1621 ttgaggcggg tattttcgcg gccgtcgaaa aacgccaggt aattttccca gtcgccgcgg
     1681 cgcgccagct cgttgagcca tttgccgcgc gcgtggtaag cgaacggaaa atcggcgtgg
     1741 cgttgcagga acatctccgc ctgctgcggg ctggttcggc cgacctgaag cttgaaagcg
     1801 tcgtattcga gatagggttt agccggataa tcgccgagct ttttaagcaa caggttgaag
     1861 cggctcagct ggttacgctg caacgcgtag gcggcatcgt ggaacaaccg gcgctgttgc
     1921 tcgtagctcg cctgtaccgt cggcgtcgcg acggcggcaa ccagcattag catcgtcgcc
     1981 agtacgcagt gattggtgga taaactgata gtcgcggaaa gcttcaattg ttgccttttt
     2041 tatcaggaag ttaggtcaaa tactaaaagt aaattatcag tagtgcaatg atcattcgcc
     2101 cgggtctcga tgcggatgcg cgagccttgc cggatcgagc aaatggcgca gccgcgattc
     2161 ctcgatatcg gtcatctcga tcgctacgtc gagtatagga cgattttccc gcattgcgcg
     2221 gttggcgatc tccgccgccc gccggtagcc gatgagcgga ttcagcgcgg tgaccaggat
     2281 cggattgcgc gccaggtttt ccgccagcac cgcctggttg acgctgaagc ggtcgatgac
     2341 cggggcgagg tggttgcagg aattggcgag aatttcgatg ctctgcagca ggttgtaggc
     2401 gatcaccggc agcatcacgt tcagctgaaa attgccggac tgcgcggcca cggcgatggt
     2461 cgcgtcgttg ccggtgacct gcgcggccac catcagcacc gcttcgggaa ttaccgggtt
     2521 gaccttgccc ggcatgatcg aactgcccgg ctgcagggct tcgaggcata tttcgccgag
     2581 gccggccaac ggccccgaat tcatccagcg caaatcgtta ctgatcttgg tcagcgaggt
     2641 ggcgaggacc cgtaactggc cgctcaactc gagcgtcgaa ttttgcgtgg cgattccctc
     2701 gaagcggtta tccatcggct tgaagtcgat tccggtctca gcccgcagga gcgcgcagac
     2761 gcggtcgccg aaatcgctgc cggtattgac tccggtgccg accgcggtgc cgccgatagc
     2821 cagccgttgc agcctggtca gggccgcctg aatgcgatag cgatcgttct tcagctgtgc
     2881 gccccaggcg gagatttcct ggcccaggga aagcggcatc gcgtccatca aatgggtgcg
     2941 cccggtcttg acggtatcgt cgagcgcctc gctgcgcccg gcgattgcgg tttcgaggtt
     3001 ctggatcgcc accagcagtt gctcgtgcac ggcgaggctg gcggcgacat ggattgccga
     3061 tgggaacacg tcgttggaac tctgccccat attgacgtga tcgttggcgt cgatctcgag
     3121 cccttcggca gacgccagcg tcgcgatgac ctcgttgaca ttcatgttgc tgctggtacc
     3181 cgagccggtc tgaaacacgt cgatgccaaa ctggtcggca tgctcgccat ccagtatctt
     3241 cgccgccgcc gcatcgattg ccgccgcgcg ttcggcatcg agctcaccga gcctcgcgtt
     3301 gactcgcgcg cccgcctgct tgagcagcgc cagcgcacga atcatagccg ccggcagacg
     3361 cagcccgctg atccggaaat tatcgagagc acgctgagtt tgcgcccggt acagggcccc
     3421 ggacgggatc tccacgctac ccagactgtc ggtcgccttg attttttttc cgcccatgct
     3481 gattgtcctt cataccccgg gacggactga ttgtaacacc acgaatagcg tcgcaatgcc
     3541 gcatcgacgg ttttttttaa ggctgggcac tcgctaccac aaaagccggt gaactggact
     3601 ataatcgggc cggtatggag agcaaaacgc gaatctttta accggtaccg aggtggttac
     3661 gatgacagac gaagatacag tagtactgac ccctgaggaa aaagaaaaga taatgaaaaa
     3721 accctccgag gataaaaagc aggatgaaga agaggacgag gactagtccc gggccgattt
     3781 gagcgtgccc gcatcgccgg gcgccaccta ccccatactc gaattcgcgc gcgcgctccg
     3841 ctgggccgcg cgtaattatt tacgggtttg accgcggtcg caagccgcac agccgcccgc
     3901 ctcaccactt tactggcgct cgactaccgt caccaccgac tcgcagagct gccgcagttc
     3961 gtcatcgctg atcacgaaag ccggcatcag gtagaccagg cggccgaatg gccgcaccca
     4021 gaccccggcg tcgacgaact gccgggtaat ttcggccatg tttacgggtt cacgcatttc
     4081 gaccacgcca atcgcaccca ggcagcgcag ctcggcgacc tgcggcagcg acgcgcacgg
     4141 cgccagccat cgcgccagat tcttctcgat ccgcgccacg cgtgcctgcc agtcggactc
     4201 cagcagtaat tcgatgctct ccagcgccac cgcgcaggcc agcggattgc ccatgaaagt
     4261 cggtccatgc atgaacacgc caggactgcc gccatcgatg gtgtcgctga tctcggtggt
     4321 ggccagagtc gcggcgaggc tcaggtagcc gccagtcagc gccttgccga ggcacaggat
     4381 gtcgggtgcg accccggcgt gctcgcacgc gaacagcttg ccgctgcggc cgaagccggt
     4441 ggcaatctcg tcggcgatca gcaatacgcc gtagcggtcg cacagttccc gcgcgcatgc
     4501 caggaactcg gccgagtaaa aatacatgcc gccggcaccc tgcactatcg gctcgaggat
     4561 cagcgcggcg atttcatcgc cgcgttcgcg cagcacggtc tccagcgcct gcgcatcgct
     4621 gtcgcgcaag gattcgccga agcgggatgc ggggcgggcg acaaaatgct gtgaggtcag
     4681 catcgtgttg aacaggctgt gcatgccggt gaccggatcg caaaccgaca tcgcccccag
     4741 cgtatcgcca tgatagccgt tgcgcagcgc cgcgaaacgc tgcttggagg gcagcccgcg
     4801 ggcattccag tactggatcg ccatcttcat cgcgacctcg acggcaaccg aacccgaatc
     4861 gcacagaaac accgat
//
LOCUS       DATCZV010000509         8620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_411978, whole genome shotgun sequence.
ACCESSION   DATCZV010000509 DATCZV010000000
VERSION     DATCZV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411978"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VIW27_11465"
     CDS             <1..373
                     /locus_tag="VIW27_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY5740338.1"
                     /translation="MTPACWPLLAGEQQLALRKFTRPTKVRTTTTKTRHSLHPENEEM
                     FAALRAHRKQLAEQQGVPPYVILHDKTLVAICELKPRSIDELASVPGIGARKLDLYGA
                     DLLELVSRYRETARAIATDTA"
     gene            656..1300
                     /locus_tag="VIW27_11470"
                     /pseudo
     CDS             656..1300
                     /locus_tag="VIW27_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
     gene            complement(1406..1864)
                     /locus_tag="VIW27_11475"
     CDS             complement(1406..1864)
                     /locus_tag="VIW27_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5740339.1"
                     /translation="MAQKSKKTHIVAFALPLFLEHGFKGTSIDMVVRASGVSKPTVYN
                     HFPDKAALMEAVLQRWIDEHKPIIAPLQGKAALQGFIREHWLTAEAVRLYAVVIGEGR
                     RFPLARKMFWEQYDRRWRVAFDYICERSPNLDRAGIDSLLDHHLLAGLRE"
     gene            1955..3214
                     /gene="trpB"
                     /locus_tag="VIW27_11480"
     CDS             1955..3214
                     /gene="trpB"
                     /locus_tag="VIW27_11480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008044820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5740340.1"
                     /translation="MIRSDNGYFGEFGGRFVPEILLPALTELEEMFFGLRDDPDFWQE
                     YATLCQEFSGRPTPLTPLRRLSKQLGGAQIWLKREDLNHSGAHKINNVLGQGLLVQRM
                     GKQRVIAETGAGQHGLATAIMSARLGLDCTIYMGAEDIERQYSNVFWMRQLGSKVEAV
                     NTGARTLKEALDEALRDWAASVESTHYVLGTACGPAPFPEMVSYFQGIISEEIREQSL
                     RQIGRLPDEVVACVGGGSNAMGAFGAFLDSPEVALVGIEAGGISLEDGQHSVRLSQNT
                     GETGIAQGYKTLFLQNDDGQLGDTHSIAAGLDYVGVSPILAYLAQQGRIVADSATDDE
                     VTEAFSLLMKVEGIIPALESSHGLAGGFRRARQMRPDQHIVINLSGRGDKDIFNIARA
                     FPQPEWTEFLQREATRATELLAQKGGH"
     gene            3217..3987
                     /gene="trpA"
                     /locus_tag="VIW27_11485"
     CDS             3217..3987
                     /gene="trpA"
                     /locus_tag="VIW27_11485"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5740341.1"
                     /translation="MFPAQYDDLQIMSHAVVGFPSIEENEKAIAGLAKAGVRLIELQV
                     PFSDPVADGHTLVNACYEALKHGGSVRATLDLAKQVSARHPEVTFILMSYLNPLYRYG
                     LEELFEAASAAGIKGMIVPDWPVEEIEPWLPTLDRLGLAPILMVTPNTAAERVARIAR
                     ASRGMIYVVSRLGVTGHETQWGEKFQNYIAEIRKHTDLPLAVGFGVRTAKDLVALAGK
                     AEVAAFCSQYIEWQRDDGSDSAADKMKSIVASLGQPGA"
     gene            4021..4347
                     /locus_tag="VIW27_11490"
     CDS             4021..4347
                     /locus_tag="VIW27_11490"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HEY5740342.1"
                     /translation="MFGLLELLLLTLLALFAVYWLQSGLYKGRARELADAHCRSLDLQ
                     LLDQGMVITGIWPVRGRDGRLQLRRRYQFEFASRGDRRYRGEIVLLGTAAQSIQLETY
                     KTPSEE"
     gene            4457..5143
                     /locus_tag="VIW27_11495"
     CDS             4457..5143
                     /locus_tag="VIW27_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5740343.1"
                     /translation="MKQLIVMLTVAGLALASGCTTFDPYTGEEKTSNTAKGAGIGAGV
                     GAVLAYVANRDESSKERKKRMLQAAGIGAIAGGGIGYYMDTQEARLRKTLRDTGVSVE
                     RDGDNINLIMPGNITFVTNGHDLNADFYEVLDSVVLVVQEFEKTLIVAAGHTDSTGSG
                     SYNQALSERRANSVASYLRSRGVVDARIETVGFGEDHPLADNGTAEGRSLNRRVELSL
                     LPISEQALGG"
     gene            complement(5145..6380)
                     /locus_tag="VIW27_11500"
     CDS             complement(5145..6380)
                     /locus_tag="VIW27_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740344.1"
                     /translation="MSPLHRNIYLYPWFRAASSGHAWIPVFFLYLSQRLSLEQVIELS
                     AVYYLSVFVLEVPSGYFSDRVGRRSTLLIAAASLIAAYICFIVGGGFAWFAAGQFLLA
                     AGIAMQSGTDTAFHYDSLKALGREGEYAAREARAEQSGMTMLALATLAGGLLGLLDLR
                     LAYLFSLVCALAMAVLVWRFSEPGHARESRLLPQSFVAVVIGCLARLREPVLGWIFLV
                     VILLYALAHIVFEFYQPYIVLLRLPLFAEQDHAPLLSGLVISISMFGGALGARASIDW
                     QARLGLLGVLAVAMLIQLGIVAAMAMAISPLVLALVCLRNFPMALVHAPIRAAIAPRI
                     GREQRATYLSLQGLAERLCFALLLLWLAARLEPGAAIAAATLHGILESTLWFGVLAAA
                     ALSIAAPRIRRLLLRDGTG"
     gene            6636..6983
                     /gene="hspQ"
                     /locus_tag="VIW27_11505"
     CDS             6636..6983
                     /gene="hspQ"
                     /locus_tag="VIW27_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEY5740345.1"
                     /translation="MEEFFSSNVTSTQFAIGQVVRHRIYPFRGVIVDVDPQFNNTEEW
                     WLAIPEEVRPRKDQPFYHLLAENETTAYEAYVSEQNLLLDETGEPVNHPQVAEVFGEF
                     LGERYALPNSYRN"
     gene            complement(7176..8150)
                     /locus_tag="VIW27_11510"
     CDS             complement(7176..8150)
                     /locus_tag="VIW27_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY5740346.1"
                     /translation="MKTVLVVQGVASVDQVPRLEELEAQAEIRLATSVDELCAALPGA
                     DALLGWNFRADNLRQAWDAADRLRWIHWGGAGVDAAMFAELVASDVVLTNARGIFDRP
                     MAEWVLGVILCFAKRIPETLASQARSSWDYRMSETVLGKRALVVGVGSIGRAIGRLLR
                     AAGMEVEAVGRRARAGGEDFSCIHAIDELHSRLPLADYVILITPLTEQTRKLFGAAEF
                     DAMAAHARFINVGRGALVVEEDLLSALNQGHIAGAALDVFVEEPLPSDSPFWQAPNCI
                     VSPHMSGDYIEYEADMAGQFIANWKRFVAGEPLANPVDKQLGFVPAAD"
BASE COUNT         1715 a         2776 c         2661 g         1468 t
ORIGIN      
        1 gatgacaccg gcctgctggc cgctgctcgc cggcgagcag caactggcgc tgagaaaatt
       61 caccaggccg acaaaagtcc gcaccacgac gaccaaaacc cgccacagcc tgcaccccga
      121 aaacgaggaa atgttcgcgg ccttgcgcgc ccatcgcaag cagctcgccg aacagcaggg
      181 cgtgccgccg tacgtgatcc tgcacgacaa gaccctggtc gcgatctgcg agctaaagcc
      241 gcggtcgatc gacgagctgg cttcggtccc tggcatcggc gcgcgcaagc tggatctgta
      301 cggtgccgac ctgctggaac tggtgtctcg ctaccgcgaa accgccaggg ccatcgccac
      361 cgataccgcc tagtggattt cggactgctg gaactgacgc cgctgcaggt aacggcgagc
      421 ggcgcgattt tcgtctggtc gggattcctg cgcgccgggt tagcccgcat ttgtcaccaa
      481 gattcgtcgc gagggcgtcg aaaagacgct gtggctggct gcgcggacca gaggtcggcg
      541 ccggcgtagt attaactacg tcaagccgtc cgccgggagc acagccagcc acagtgcgct
      601 ttgcggcgtc cgcagcagaa gattggtggc aaatgcgggc tagggtttcg gcggcgcggt
      661 gctgaccctg ccgctgctgc tggctgtcga cgaccggcca ctgttctggc taccgatcct
      721 ggcctgccac ctgctgctgt tcacggcgat cacgctgcgc tcgcgtctcg gcaacgtcga
      781 ctggcacgga ctcaaagcgt cactgccatg gatcatgccg gcgaagctga tcggcgtgtt
      841 cggtctgctg agcatgccca accgctggct cctgacgatt atttacggca tcacgatatt
      901 ttacgcgctg ctgtggatct cgggcgcgcg catcgagagc cgcaaaccct ggctcgacaa
      961 gctgatgctg ctcggcggcg gctatttctc cggcacctcg ctttccggcg caccgttgat
     1021 cgtcgcggtc tacgtcaagc gtgtcgcccg ccgcagcctc agggacacca tgttcgtgct
     1081 ctggttcctg ctggtatcga tcaagttggc gacgctggcc tggttcggca tcgacctgca
     1141 ggcaggcagc atgctgttgc tggtgccggt taccgggctg gggcacgcga tcggcatgag
     1201 agtgcacgac aacctgctgg aaaacgatcg gctgttcaag cgcgtgctcg gtgtcggcct
     1261 gctggcgatc gccgggctcg gtgtcgcgcg cagctggtag gccggcgacg acattcgccc
     1321 ttcgctcggc agcgcggccg acggggaacg acgacccggc cgctgaattc ctctaccttc
     1381 ggcctgcggc gcctcgatga ccgcgctatt cgcgcaatcc agcgagcagg tggtggtcga
     1441 gcaacgagtc gatgccggcc cggtcgaggt tcggcgagcg ctcgcaaata tagtcgaagg
     1501 ctacccgcca acgccggtca tattgctccc agaacatttt ccgcgccagc ggaaagcgcc
     1561 taccctcacc gataaccacc gcgtacaggc gcacggcctc ggcagtcagc cagtgctcgc
     1621 gtatgaagcc ttgcagagca gctttgccct gcagtggcgc gataatcggc ttgtgctcgt
     1681 cgatccagcg ctgcaaaacg gcttccatca gcgccgcctt gtcgggaaaa tgattgtaga
     1741 cggtgggttt ggagacaccg ctggccctga ccaccatatc gatcgacgtg cccttgaaac
     1801 cgtgttcgag aaacaacggc agcgcgaatg cgacgatatg ggttttcttg gacttctgcg
     1861 ccacgcgggg ttgattatcc gtttgaattg tgcaaactat actaaaccgt ttagttaatg
     1921 tcattaaccg cgtactaacc cgccaggaga taccatgatt cgcagcgata acggctattt
     1981 cggcgagttc ggcggacgtt tcgtccccga aatcctgcta cccgctttga ccgagctcga
     2041 agaaatgttc ttcggcctgc gcgacgatcc cgatttctgg caggaatacg caaccctgtg
     2101 ccaggagttc agtggccggc cgacgccgtt gacgccgttg cggcggctgt cgaaacagct
     2161 cggcggtgcg caaatctggc tgaagcgtga agacctgaac cattccggcg cgcacaagat
     2221 taacaacgtc ctcggccagg gattgctggt gcagcgcatg ggcaagcagc gcgtgatcgc
     2281 cgaaaccggc gccggacagc acggcctcgc gaccgcgatc atgtccgcca ggctggggct
     2341 cgactgcacc atctacatgg gtgccgagga tatcgagcgc cagtattcga acgtcttctg
     2401 gatgcgtcag ctcggctcga aggtggaagc ggtcaatacc ggcgccagga ccctcaaaga
     2461 agcgctcgac gaggcgctgc gcgactgggc tgcgtcggtg gagagcacgc actatgtgct
     2521 cggcacggcc tgcgggccgg cgccgtttcc ggagatggtg tcttatttcc agggcatcat
     2581 cagcgaggag attcgcgagc agtcactgcg ccagatcggc cgcctgcccg acgaggtcgt
     2641 cgcctgcgtc ggtggcggtt ccaacgcaat gggcgccttt ggcgcttttc tcgacagtcc
     2701 cgaggtggcg ctggtcggca tcgaggccgg cggcatcagc ctcgaggatg gccagcattc
     2761 ggtgcgcctg tcgcaaaaca ccggcgaaac cggcatcgcg cagggctaca aaacgctatt
     2821 cctgcagaac gacgacggcc agctcggcga tacgcacagc atcgccgccg ggctcgatta
     2881 cgtcggcgtg tcgccgatcc tcgcctacct cgcgcaacag ggtcgcatcg tggctgattc
     2941 ggccaccgac gacgaggtca ccgaggcctt cagcctgttg atgaaggtcg agggcatcat
     3001 tccggcgctg gaatcgtcgc acgggctcgc cggtggtttc aggcgcgcgc ggcaaatgcg
     3061 tcccgaccag cacatcgtaa tcaacctcag cggccgcggc gacaaggata tcttcaatat
     3121 tgcgcgcgcc tttccgcagc cggagtggac agagttcctg cagcgcgagg cgacccgcgc
     3181 taccgagttg ctggcgcaaa agggagggca ctagacatgt ttccggcaca gtatgacgac
     3241 ctgcaaatca tgtcccacgc ggtggtcggc tttccgagca tcgaggaaaa cgaaaaagcg
     3301 atcgccgggc tggccaaagc cggcgtacga ctgatcgagc tgcaggtgcc cttcagcgac
     3361 ccggtcgccg acggccacac gctggtcaac gcctgctacg aggcactcaa gcacggcggt
     3421 tcggtgcgcg caaccctcga cctggcgaag caggtaagcg cccgtcaccc cgaggtgact
     3481 tttatcctga tgagctacct gaatccgctg taccgctacg gcctagaaga attgttcgag
     3541 gcagcgtccg ctgccggtat caagggcatg atcgtacccg actggccggt cgaggaaatc
     3601 gaaccctggc tgccgacact cgaccggctg ggcctggcgc cgatcctgat ggttaccccg
     3661 aacaccgcgg ccgagcgggt ggcacgaatc gcgcgcgcct cgcgcggcat gatctacgtg
     3721 gtttcgcgac tcggcgtgac cggccacgaa acccagtggg gagaaaaatt ccagaactac
     3781 attgccgaaa tacgcaagca taccgacctg ccgctggcgg tcggcttcgg cgtgcgtacc
     3841 gcgaaagatc tcgtcgcgct cgccggcaag gccgaggttg ccgcgttttg ctcgcagtac
     3901 atcgaatggc agcgcgacga cggcagcgac agcgccgccg ataaaatgaa atcgatcgtc
     3961 gcctcgctcg ggcagccggg cgcctgaccc ggcggctcgc actgcagcgg ccgcggcgca
     4021 atgttcggcc tgcttgaatt gctgctgttg acgctgctcg cgctgttcgc ggtctactgg
     4081 ttgcagagcg gtctttataa ggggcgggcc cgtgaactgg ctgacgccca ctgccgcagc
     4141 ctggatctgc aactgctcga tcagggcatg gtgattaccg gcatctggcc ggtacgcggc
     4201 cgtgatggcc gcctgcagct gcggcgccgc taccagttcg agttcgcgtc gcgcggcgac
     4261 cgccgctacc gcggcgagat cgtgctgctc ggcacggcgg cgcaatcgat ccaactcgaa
     4321 acctataaaa cgccgtcgga agagtaattt cagccgcgcg acgaacggcc ggcaaggttc
     4381 aggtcgcatt caccagccct ggtctatact cctctccagc ctttccgtat tcagactgga
     4441 gtttttcgga gcaaccatga agcaactgat cgtcatgctg accgtggccg gcctcgccct
     4501 ggcgtccggc tgtaccacct tcgaccctta taccggggaa gaaaagacct ccaataccgc
     4561 caagggtgcc ggcatcggcg ccggcgtcgg cgcggtgctc gcctatgtcg ccaatcgcga
     4621 cgaaagcagc aaggaacgca agaaacgcat gctgcaggcg gccggcatcg gcgccatcgc
     4681 cggcggtggc atcggttact atatggatac ccaggaagcc aggctcagga agacgctgcg
     4741 cgacaccggt gtcagcgtcg aacgcgacgg cgataacatc aacctgatca tgcccggcaa
     4801 cattaccttc gtcaccaacg gccacgactt gaatgccgat ttctacgagg tactggattc
     4861 ggtggtgctg gtggtgcagg aattcgagaa aaccctgatc gtggccgccg ggcacaccga
     4921 cagcaccggc tccggcagtt acaatcaggc gctgtcggag cggcgcgcga attcggtggc
     4981 cagctacctg aggagtcgcg gcgtcgtcga cgcgcgcatc gagaccgtcg gtttcggcga
     5041 agaccatccg ctagccgaca atggcaccgc ggagggccgc agcctcaatc gccgggtcga
     5101 actgtcgctg ctgccgattt ccgagcaggc cctcggcggt tagcttagcc ggtgccgtcg
     5161 cgcagcagca gccggcgaat ccgtggcgcg gcgatggaca gcgcggcggc cgccagcacg
     5221 ccgaaccaaa gcgtcgactc gagaatgccg tgcagggtcg ccgcggcgat cgccgcgccg
     5281 ggttccagcc gggccgccag ccacagcagc agcaacgcga aacacaggcg ctcggccagc
     5341 ccctgcaacg acaggtaggt ggcgcgctgc tcgcgaccga tacgcggcgc gatcgcggcc
     5401 ctgatcgggg catgtaccag cgccatcgga aaatttctca ggcacaccag tgccagcacc
     5461 agcgggctga tcgccatcgc catcgccgcg acgatgccga gctgaatcag catcgccacg
     5521 gcgagcacgc caagcagccc gagccgggcc tgccagtcga tgctggcgcg cgcccccagc
     5581 gcgccgccga acatcgaaat cgagatcacc agccccgaca gcagcggcgc gtggtcctgc
     5641 tcggcaaaca gcggcagacg tagcaacacg atatacggct ggtaaaactc gaacacgatg
     5701 tgcgccagcg cgtagagcag tatgacgacc agaaatatcc agccgagcac cggctcgcgc
     5761 agccgagcca ggcagccgat caccaccgcg acgaaactct gcggcaacag ccgagattcg
     5821 cgcgcgtggc ccggttcgct gaagcgccag acgagcaccg ccatggccag cgcacagacc
     5881 agcgagaaca ggtacgcgag acgcaggtcg agcaggccca gcaaaccgcc ggcgagcgtc
     5941 gccagcgcca gcatggtcat gcccgactgc tcggcacggg cctcgcgcgc ggcatactca
     6001 ccctcgcgac cgagtgcctt cagcgaatcg tagtgaaacg cggtatcggt gcccgattgc
     6061 atggcgatac cggcagcgag caggaattga ccggccgcaa accaggcgaa gccgccgccg
     6121 acgatgaagc agatataggc ggcgatcagc gaggccgccg cgatcaacag cgtgctgcgc
     6181 cggccgacac gatcagagaa gtatcccgaa ggtacttcga gcacgaaaac cgacaggtag
     6241 tagactgccg acagttcgat cacctgctcg agcgacaacc gctgactcag gtagagaaaa
     6301 aacaccggga tccacgcatg gccgctggat gccgcgcgaa accagggata gagataaata
     6361 ttgcggtgta atgggctcaa gcgtcgatca ccgattttgc agccgggctc aggcatcgac
     6421 gccgattcaa gggttcgtgt cgccgtcgcc gcgaacacgg ccgacacgcg aaaactcgct
     6481 cattcggctg ctgcatggat gacttgcgcc ccgcgggtgg ctattattga ccggcgcgaa
     6541 gccgcacgca atatcggcgc aattgctgtg aatcggttcg cgtagacggt agaaagacgt
     6601 tgatttaatg ccattgagac ctatatatcg gtactatgga agaattcttt agcagcaacg
     6661 taaccagcac gcaattcgcg atcggacagg tcgtgcgtca tcgcatttac ccgtttcgcg
     6721 gtgtcatcgt cgacgtggat ccccagttca acaacaccga ggagtggtgg ctcgcgattc
     6781 ccgaggaagt gcgcccgcgc aaggaccagc ctttttacca cttgctggcg gaaaacgaaa
     6841 ccaccgccta cgaggcctat gtttccgagc aaaacctgct gctcgacgag accggcgagc
     6901 cggtcaacca tccgcaggta gccgaggtat tcggtgaatt tctcggcgag cgctacgcgc
     6961 tgcccaacag ctaccgcaac tagcccgtat atcaccccgc tttcccgctg cggacgccgc
     7021 aaaaggtacg ctttcgcacc ataaaccggt ttgctatgcg tgcttgccct gatacttgcc
     7081 tgatcctcgg ctgcgaatgc cgcaacaatc catgcgcgcg agggtcgacg aaactggacg
     7141 cttgccgccg ccggcgaaaa taatgtcaat tgcggtcaat ccgcggccgg cacgaatccg
     7201 agttgcttgt cgaccggatt ggcgagcggt tcgccggcca cgaaacgctt ccagttggcg
     7261 atgaactggc cggccatatc ggcctcgtac tcgatgtaat cccccgacat gtgcggtgac
     7321 acgatacagt tcggcgcttg ccagaaaggg ctgtcggacg gcagcggctc ttcgacgaac
     7381 acgtcgaggg cggcgccggc gatgtggccc tggttcaatg ccgacagcag gtcttcctcg
     7441 actaccaacg cgccacggcc gacattgatg aaacgcgcat gcgcggccat cgcgtcgaat
     7501 tcggcggcgc cgaacagctt gcgggtctgc tcggtcagcg gcgtgatcag gatgacataa
     7561 tccgccagcg gcagccgcga gtgcagctcg tcgatcgcgt gaatgcacga gaagtcctcg
     7621 ccgccggcgc gcgcgcggcg gccgactgcc tcgacttcca tgcccgcggc ccgcagcagg
     7681 cggccgatgg cgcgaccgat cgaaccgaca ccgaccacca gcgcgcgctt gccgagcacg
     7741 gtttcgctca tgcggtaatc ccagctcgaa cgagcctggg acgcaagagt ttcgggaatg
     7801 cgcttggcga agcaaagaat cacacccagc acccactcgg ccatcggccg gtcgaagatg
     7861 ccgcgggcat tggtcaacac gacatcgctg gcaacgagtt cggcaaacat cgccgcatcg
     7921 acgccggcgc cgccccagtg tatccagcgc agccggtccg cggcgtccca ggcctggcgc
     7981 aggttgtcgg cgcggaagtt ccagcccagc agtgcgtcgg ccccgggtaa agccgcgcac
     8041 aactcgtcta ccgaggtcgc gaggcggatc tccgcctgcg cttcgagttc ctcgagacgc
     8101 ggcacctgat cgacgcttgc gacaccctgc accaccaaca ccgttttcat ctgcttcacc
     8161 tccagcgaat tcgaaacatc attcggcgac ccgacacaag gttctgcccg gttcgaacaa
     8221 caagcataat cactgcggcg tggccagtcc atgcaggcct cggcaatcga caatcgagca
     8281 tatggacaaa tatcgcgaac aggcgcaaac tgcgcgctgc cggaggctta ccgccggcag
     8341 cggcgggtca tcaccgctcg cgcgaaaact aactacgcgc tcaacaggga tagccgtact
     8401 gccattccgc ggcatcctgt cagaacggac gttagacact tttgttctca gctacttagg
     8461 tgtttatgtc tatgaaaaag ccgaccaccg gcccggccag gcctggcaat cgcaatcagc
     8521 gcctgatcga agtcctcgga cccatcgaaa atctcgaaag ttatgtcaag gccgactggt
     8581 ggcggcatat cttcaacgcc aattacctgc gcaccgacgg
//
LOCUS       DATCZV010000510         9541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_517536, whole genome shotgun sequence.
ACCESSION   DATCZV010000510 DATCZV010000000
VERSION     DATCZV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517536"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VIW27_11515"
     CDS             complement(<1..296)
                     /locus_tag="VIW27_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY5740347.1"
                     /translation="MTIEKSQIEGILGGIVDVNMEADLVASKAVKSIEVDGNNCRIAI
                     RLGYPAAGYFDELRQQIVDAVSALDGIGEIGVEIESQVKSHAVQQNLKPLQGIK"
     gene            444..2474
                     /gene="metG"
                     /locus_tag="VIW27_11520"
     CDS             444..2474
                     /gene="metG"
                     /locus_tag="VIW27_11520"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY5740348.1"
                     /translation="MSSETRRILVTSALPYANGPIHIGHMLEYVQTDIWVRFQKLRGH
                     DCIYVCADDAHGTPIMLRAQAEGVSEQELIDRMHVEHLRDFTDFGVDFDNYYSTHSDE
                     NRYFAEYIYTQLRAAGHIRTRVIKQAYDPKAEMFLPDRYIKGECPNCGSGDQYGDNCE
                     VCGATYDPTELKNPLSVVSGEAPIEKDSEQYFFKLEDFEQMLKDWFAAGHVQTEIANK
                     MNEWLDEGLQDWDISRNAPYWGFEIPDAPGKYFYVWLDAPIGYMASFKNWCEANDRDF
                     DQYWNADSEAELYHFIGKDIARFHTMFWPAMLHGAGFRMPSAVFCHGFLTVDGQKMSK
                     SRGTFIMARTYLDHLNPEYLRYYFAAKLGPGIDDIDLSLDDFTQRVNSDLVGKLVNIA
                     SRCAGFINKRFDGKLAARLSSDALSRQIATAGEVIADSYEKREFGRAMRDIMALADKV
                     NQYIDEQQPWVAIKDEARHGEVQDVCSIGLNAFRQLVILLKPVLPRLAAEAEAFLQVD
                     DLRWADLDRDLLDHGIARFKPLMTRIEPVFIERMIEASKEGAAAEQKIQAGVGDASIE
                     PIAPEIAYDDFAKLDFRVARIVAARRVEKSDKLLQLTLDIGSETRNVFAGIAGAYEPE
                     DIEGRLTIMVANLAPRKMRFGVSEGMVLAAGDDRGLYLLSPDSGAEPGMRVS"
     gene            2479..3738
                     /locus_tag="VIW27_11525"
     CDS             2479..3738
                     /locus_tag="VIW27_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY5740349.1"
                     /translation="MSRIIAFAWLLAGFLAPPAAALDSRVIFEMHEQRVLQIRVLNRE
                     TGKKSSIGSGFVVGDGRQVATNYHVIGKVILEPEEFYISYLDKDGREGELKLLGLDVA
                     RDLALLGAEEPFGEPVMMSPLPARGAPIFAMGNPLDLGLTIAVGTNGGILNQTDDSRI
                     LFSGSLNPGMSGGPTFNDQGEVIGINVATARNDISFIVPSRFLIDLVEQATAAGFDSS
                     EDLRDEVARQVLEYENHYIEQLLAADWPDLDLRQFRLPGIISPTVRCWDNSARLSTTA
                     RYRRLQIRCSNQNHIYLDEQNKFVGNLVYEYYWLETDELNPTQFYNLYEYLNKDQIDS
                     KLDRDVVSNFNCATWFVNIAGQEFKTNLCRREYVEYPGLSDVLFTAAMTGFDDRGLFI
                     TLTATGVDYAASLPLIQKFLEQVEWRP"
     gene            3726..4682
                     /locus_tag="VIW27_11530"
     CDS             3726..4682
                     /locus_tag="VIW27_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5740350.1"
                     /translation="MASLILEIFTRGLHRYQPLEKQVTTIGRALDNDVILSDPTVAPY
                     HARIVRHHDDALEIINLAEVNPTRVGRRRVDSLVIPKLPVMLDIGRVQLQVLNRDQPV
                     AATRPLAGSDRRGHLFAHAYWAVLLLLACLALGGLEFYQSAYNSFKWSDLLKYVMRET
                     MLTLGVLILSLAVLERLLVHRWEIRQLVTAVALALLVYHATGWLADMLDYLFSARWPG
                     TLLYFGWYLLAVPGAIALYLIHISHFRHGRSLLLAILIASPIAVPGLLQSPRLHALLD
                     DFSSSASYHNQLSALNLHLAERVSIETFIAEAGELEAGEFAN"
     gene            4776..5357
                     /gene="rsxA"
                     /locus_tag="VIW27_11535"
     CDS             4776..5357
                     /gene="rsxA"
                     /locus_tag="VIW27_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEY5740351.1"
                     /translation="MSEYLLLLIGTVFVNNIVLAKFLGLCPFMGVSRKVETAIGMSAA
                     TTFVLTLSAVLSYMINSWLLAPLGLEYLRTIAFIIAIAAVVNFTEMVVHKTSPLMYNV
                     LGIFLPLITTNCAVLGVALINVQQHNSFIESVIYGFGAALGFSLVLVLFAALRERINV
                     ADVPAPFRGSAIGLVTAGLMSLAFMGLGGLVKA"
     gene            5361..5924
                     /gene="rsxB"
                     /locus_tag="VIW27_11540"
     CDS             5361..5924
                     /gene="rsxB"
                     /locus_tag="VIW27_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEY5740352.1"
                     /translation="MLAAILTIGLMAGSFGLILGYASVRFRVEGNPIVEQIDALLPQT
                     QCGQCTYAGCRPYAEAIANGEAPINRCPPGGQATIVALADLLDTEVLELNEELGEHLE
                     VPLVAVIDEQTCIGCTLCIQACPVDAILGAAKHMHTVIADECTGCKLCLPPCPVDCID
                     MVPEKRTIDDWKWSLPEDLPQQVKHAG"
     gene            5914..7470
                     /gene="rsxC"
                     /locus_tag="VIW27_11545"
     CDS             5914..7470
                     /gene="rsxC"
                     /locus_tag="VIW27_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEY5740353.1"
                     /translation="MPVRLHRFHGGLRLEGHKQESLTRGIEPATLPAQLILPLRQHIG
                     DYNEPRVKVGARVLKGEAIASSSSSISAALHAPSSGIVSAIEHRPIAHPSGQQDLCMV
                     IDVDGEDRALPMAGADLQQLSAAEMLQRIRDAGIAGLGGAAFPSEPKLARGHGNGIDT
                     LIINGVECEPYITCDDVLMRERAAEILHGCSYLQRLLEPRCTLIAIEDNKPEATAAMT
                     RALDAAPLAATEIVVIPTIYPAGGEKQLIQVLTGKEVPHGQLAFDIGLLCHNVGTCVA
                     IARALEIGEPQISRIVTVSGNNVERPGNWEVRFGTPVGHLLEQAGGYRHGEDAWLVMG
                     GPMMGFALSGDRVPIVKASNCILAMREQTIPHAVGIHDECIRCGKCTEVCPARLLPQQ
                     LYWHARARNYDRTREFHLFDCIECGCCSAVCPSGIPLVQYYRAAKSEIRAAQKAQFKS
                     DRARVRFEFREKRLLLKKQQDEERKRLKREALQQRRARSADQAAVPDPVQAALERVKE
                     RQKATQDEPS"
     gene            7467..8489
                     /gene="rsxD"
                     /locus_tag="VIW27_11550"
     CDS             7467..8489
                     /gene="rsxD"
                     /locus_tag="VIW27_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HEY5740354.1"
                     /translation="MSRVLVSSPYVAPGNSLQRLMIHVLVALLPGTLAYSWHFGPGVL
                     VNIVLAIVFALAFEAGILALRKRPVMPQLTDFSAVVAAWLFALCLPMHSPWWLVLVGM
                     GFAMVAGKHLYGGLGFNPFNPAMVGYVVLLISFPREMTTWYQPGALGGETLGLADSLA
                     YSFGSADIGQWDALSSATPLDLVRTGLGQDLPLSEIRNSPLFGNWGGSGWEWIALCWG
                     LGGIYLLATRTIRWQIPVSLLAAVLAMSLVFNLLDPEHYASPLFHLLSGAVIIGAFFI
                     ATDPVSAATTDTGRLCYGAMIGILIYVIRSWGGYPDGVAFAVLLANMCVPLIDYYTQP
                     RVYGER"
     gene            8630..9265
                     /gene="rsxG"
                     /locus_tag="VIW27_11555"
     CDS             8630..9265
                     /gene="rsxG"
                     /locus_tag="VIW27_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEY5740355.1"
                     /translation="MRATYRRQILLSGLFLLLFAVVGTALVALTEFANRDAIAANERR
                     VLLRNLYALLPASEHDNDLAEDTIRLPPAPLLGTEGASTAYRARLSGEPVAVIFNSIA
                     PDGYSGRIHLLVGVYVDGSVAGVRVVKHAETPGLGDAIEVRKSPWIRGFDGKSLQKPQ
                     LEDWAVTRDGGEFDQLTGATITPRAVVNAVRDTLLYYRRNADMLFQMKATS"
     gene            9262..>9541
                     /locus_tag="VIW27_11560"
     CDS             9262..>9541
                     /locus_tag="VIW27_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HEY5740356.1"
                     /translation="MTTHSEITRSGLWSNNVALVQLLGLCPLLAVTSTVVNGLGLGIA
                     TLITLVLSNTSVSLIRDLVRKEVRLPVFVLIIASVVTIIELGMKALFYD"
BASE COUNT         1747 a         3071 c         2928 g         1795 t
ORIGIN      
        1 ttcttgatgc cctgcagcgg cttcaggttt tgctgcacgg catgcgactt gacctggctt
       61 tcgatctcga ctccgatttc gccgataccg tcgagcgcgg ataccgcgtc gacgatctgc
      121 tggcgcaact cgtcgaaata accggccgcc gggtaaccca ggcgaatcgc gatcctgcaa
      181 ttgttaccgt cgacttcgat cgacttgacg gccttgcttg cgaccaggtc ggcttccatg
      241 ttgacgtcga ctatgccgcc aagtattcct tctatctgtg atttttcgat ggtcatcttc
      301 gttcagtgaa tgtggggtgc ctgcgagagg gcgcatttta cccgagccgg cggccaaata
      361 tcacctgctt tttatgggtt tgttctatac agccccgaag cgacccccta aaatacgctg
      421 ctttcagtat ccacaccgag ttgatgagtt ccgagacccg ccgaatcctg gtcaccagcg
      481 cgctgcccta tgccaatggc ccgattcaca tcggccacat gctcgagtac gtgcagaccg
      541 acatctgggt gcgctttcag aaattgcgcg gccacgactg catttacgtt tgcgccgacg
      601 atgcgcatgg cacgccaatc atgctgcgcg cacaggccga gggggtgagc gagcaggaat
      661 tgatcgaccg catgcacgtt gagcacctgc gcgatttcac cgatttcggg gtcgatttcg
      721 acaattacta cagcacgcat tccgacgaaa atcgctactt cgccgagtac atctacacgc
      781 agttgcgcgc agccggacat atccgtaccc gcgtaatcaa gcaggcctac gatccaaagg
      841 cggaaatgtt cctgcccgac cgctacatca agggcgaatg cccgaactgc ggctcgggtg
      901 accagtatgg cgataattgc gaggtctgcg gcgcgaccta cgatccgacc gaactgaaaa
      961 acccgctctc ggtggtcagc ggcgaggcgc cgatcgaaaa ggattccgag cagtatttct
     1021 tcaagctcga ggatttcgag caaatgctca aggactggtt cgcggccggt cacgtgcaga
     1081 ccgagatcgc caacaagatg aacgaatggc tcgacgaggg cctgcaggac tgggacattt
     1141 cgcgcaatgc gccctactgg ggattcgaga ttcccgacgc gccaggcaag tacttctacg
     1201 tctggctgga tgcgccgatc ggttacatgg cgagtttcaa gaactggtgc gaggccaacg
     1261 accgcgattt cgaccaatac tggaacgcgg acagcgaggc ggaactctac catttcatcg
     1321 gcaaggatat cgcgcgcttt cacaccatgt tctggccggc gatgctgcac ggcgccggct
     1381 tccgcatgcc gagcgcggtt ttctgccacg gtttcctgac cgtcgacggg caaaaaatgt
     1441 cgaagtcgcg cggtaccttc atcatggctc gcacctacct cgatcacctg aatcccgagt
     1501 acctgcgcta ttactttgcc gccaagctcg gtcccggcat cgacgacatc gacctgagcc
     1561 tcgacgattt cacccagcgc gtcaattccg acctggtcgg caagctggtc aacatcgcca
     1621 gccgctgtgc cggcttcatc aacaagcgtt tcgacggcaa gctcgcggca cgcctgtcca
     1681 gcgacgcgct ctcccggcag attgcgacgg ccggcgaggt catcgccgac agctatgaga
     1741 aacgcgagtt cggccgcgcg atgcgcgaca tcatggcgct cgccgacaag gtcaaccagt
     1801 acatcgacga gcaacagccc tgggtcgcga tcaaggacga ggcacgccac ggcgaggtac
     1861 aggatgtctg cagcatcggg ctcaacgctt ttcgccagct ggtgatcctg ctgaaaccgg
     1921 tactgccgcg gctggcggcc gaggccgagg cttttctgca ggtcgacgac ctgcgctggg
     1981 cggatctcga ccgcgacctg ctcgatcacg gcatcgctcg tttcaagccg ctgatgacgc
     2041 gtatcgagcc ggtgttcatc gagcgcatga tcgaggccag caaggaaggt gcagccgccg
     2101 agcagaaaat tcaagcgggc gtcggcgatg catcgatcga accgatcgcg cccgaaatcg
     2161 cctacgacga tttcgccaaa ctcgattttc gcgtggcccg catcgtcgcc gccagacgag
     2221 tcgagaaatc cgacaagctg ctgcagctga cgctcgatat cggcagcgag acgcgcaacg
     2281 tgttcgccgg catcgccggg gcctacgagc ccgaggatat cgagggccgg ctgacgataa
     2341 tggtcgccaa cctggcgccg cgcaagatgc gtttcggcgt ttccgagggc atggtgctgg
     2401 ccgccggtga cgaccggggg ctttacctgc tgagcccgga ttcgggtgcc gagccaggaa
     2461 tgagagtcag ttagattgtt gtctagaata atcgcctttg cctggttgct ggcgggattc
     2521 ctcgccccgc cggcggcggc actggattcc cgcgtcatct tcgaaatgca cgaacaacgc
     2581 gtgctgcaga tacgcgtgct gaatcgcgag accggcaaaa aatccagcat cggctccggc
     2641 ttcgtggtcg gcgacggccg ccaggtggcg accaattacc atgtcatcgg caaagtcatc
     2701 ctcgagcccg aggaattcta catcagctat ctcgacaagg acggcaggga aggtgagctg
     2761 aaactgctcg gcctcgacgt cgcccgcgac ctggcgttgc tcggtgccga ggagccgttc
     2821 ggcgaaccgg tgatgatgag cccgctgccg gcgcgcggcg cgccgatctt cgcgatgggc
     2881 aacccgctgg acctcgggct gacgatcgcc gtcggcacca acggcggcat tctcaatcag
     2941 accgacgaca gccgaatcct gttttccggc agcctcaatc caggcatgag cggcggtccg
     3001 accttcaacg accagggcga ggtcatcggt atcaacgtcg ccaccgcgcg caacgatatc
     3061 agtttcatcg taccatcgcg cttcctgatc gatctcgtcg agcaggccac cgctgcgggt
     3121 ttcgacagtt ccgaggatct cagagacgag gtcgcgcgcc aggtcctgga gtacgagaac
     3181 cactatatcg agcagctgct ggcggccgat tggccagatc tcgacctgcg ccagttcagg
     3241 ctgccgggca ttatttcgcc gaccgtgcgc tgctgggata acagcgcgcg gctcagcacc
     3301 acggcgcgtt atcgacgcct gcagattcgc tgcagcaatc agaaccatat ttacctcgac
     3361 gagcagaaca agttcgttgg caacctggtt tacgagtact actggctgga aaccgacgaa
     3421 ctgaacccca cccagttcta taacctctac gagtacctga acaaggatca gatcgactcc
     3481 aagctagacc gcgacgtggt cagtaatttc aactgcgcga cctggttcgt caacatcgcc
     3541 ggccaggaat tcaagaccaa cctgtgccgg cgcgaatacg tcgagtaccc gggcctcagc
     3601 gacgtgctgt tcacggcggc gatgaccggc ttcgacgacc gtggcttatt catcactctg
     3661 accgcgaccg gggtcgacta tgccgccagc ctgccgctga tacaaaaatt cctggagcag
     3721 gtcgaatggc gtccctgata ctggaaatct ttacccgcgg cctgcaccgt taccagccgc
     3781 tggaaaagca ggtcaccacc atcgggcgag cgctcgacaa cgacgtgatc ctgtcggacc
     3841 cgactgtggc cccgtaccat gcgcgaatcg tccgccacca cgacgacgcg ctcgaaatca
     3901 tcaatctggc cgaggtcaac ccgactcgcg tcggccggcg tcgcgtggat tcgctggtga
     3961 tccccaagct gccggtgatg ctggatatcg gacgcgtgca gcttcaggtc ctgaaccgcg
     4021 accagccagt tgcggcgacc cgtccgctgg ccggcagcga ccgccgcggt cacctgttcg
     4081 cccatgccta ctgggcggta ctgctgttgc tggcctgcct ggcgctcggc ggtctcgagt
     4141 tttatcagag cgcctataac agcttcaagt ggagcgacct gctgaagtac gtgatgcgcg
     4201 agacgatgct aaccctcggt gtgttgatcc tgtcgttggc ggtgctcgaa cgactgctgg
     4261 tgcatcgctg ggaaatcagg caactggtga ccgcggtagc gctggccttg ctggtttacc
     4321 acgccaccgg gtggctcgcc gacatgctgg attacctgtt ttcggcacgc tggcccggga
     4381 cgctgctgta cttcggctgg tacctgctag cggtgcccgg agcgattgcg ctctacctga
     4441 tacacatctc ccattttcgg cacgggcgca gcctgctgct cgcgatcctg atcgccagcc
     4501 cgattgcggt gcccgggctg ctgcaaagcc cgcgcctgca tgcgctgctc gacgatttca
     4561 gctcgtcggc tagctaccac aatcaactca gcgctctgaa tctgcacctc gccgaacgag
     4621 tttcgatcga aaccttcatc gccgaggccg gggaactcga ggccggtgag ttcgccaact
     4681 gaacgcgacc ggccgtcgcc gtcatcacag acaagccgat taaccctttg ccaatgcggt
     4741 atttttcgct caggtgagac cgtataatcc ggcccatgtc tgaatacctg ctgctattga
     4801 tcggcaccgt tttcgtcaac aacatcgtgt tggcgaagtt cctcggcctg tgtccgttca
     4861 tgggcgtatc gcgcaaggtc gaaaccgcga tcggcatgag cgcggccacc actttcgtgc
     4921 tgacgctgtc ggcggtactc agctacatga tcaacagctg gctgctggcg ccgctggggc
     4981 tcgagtacct gcgcaccatc gccttcatca tcgccatcgc cgcggtggtc aatttcaccg
     5041 agatggtggt gcacaagacc agcccgctga tgtacaacgt gctcggcatc ttcctgccgc
     5101 tgatcaccac caattgcgcg gtgctcggag tggcgctgat caacgttcag caacacaaca
     5161 gcttcatcga atcggtaatc tacggcttcg gcgcggcgct cggcttctcg ctggtgctgg
     5221 tgctgttcgc ggcactgcgg gaacgcatca acgtcgccga cgtgccggca ccgtttcgcg
     5281 gctcggcaat cggcctggta accgccggcc tgatgtcgct ggctttcatg ggcctcggcg
     5341 ggctggtcaa ggcctaggcg atgttggcgg cgatccttac catcggcctg atggccggct
     5401 cgttcggcct gatactcggc tacgcctcgg tgcgttttcg cgtcgagggc aacccgatcg
     5461 tcgagcagat cgacgcactg ttgccgcaga cgcaatgcgg ccagtgcacc tatgccggct
     5521 gccgccccta cgccgaggcg atcgccaacg gcgaggcacc gatcaatcgc tgtccgcccg
     5581 gcggccaggc gaccatcgtc gcgctcgccg acctgctgga taccgaggtc ctggaactca
     5641 acgaggaact gggcgagcac ctggaagttc cactggtcgc ggtgatcgac gagcaaacct
     5701 gcatcggttg cacgctgtgc atccaggcct gcccggtgga tgcgatcctc ggcgccgcca
     5761 aacacatgca cacggtgatc gccgacgagt gcaccggctg caagctgtgc ctgccaccct
     5821 gcccggtcga ttgcatcgat atggtgcccg aaaagcgcac catcgacgac tggaaatggt
     5881 cgctgcccga ggacctcccg cagcaggtga agcatgccgg ttaggctgca ccgttttcac
     5941 ggcggcctgc ggctcgaggg ccacaagcag gaatcgctga cccgcggtat cgaaccggca
     6001 acgctgcccg cgcagttgat tctgccgctg cggcagcata tcggcgatta caacgagccg
     6061 cgggtcaagg tcggcgcgcg cgttctcaag ggcgaagcca tcgcctcgag ctcgtcgtcg
     6121 atcagcgccg cgctgcacgc gccgagttct ggcatcgtca gcgccatcga gcaccggccg
     6181 atcgcgcacc cgtcggggca gcaggacctg tgcatggtga tcgacgtcga cggcgaggac
     6241 cgggcgctgc cgatggcggg ggccgacctg caacagctgt ccgccgcaga gatgctgcaa
     6301 cggattcgcg acgccggcat cgccggcctc ggcggcgcgg cgtttccgag tgaaccgaaa
     6361 ctggcgcgcg gccacggcaa cggcatcgat acgctgatca tcaacggcgt cgagtgcgaa
     6421 ccctatatca cctgcgacga cgtgctgatg cgcgagcgtg ccgccgagat cctgcacggc
     6481 tgcagctacc tgcagcgcct gctcgagccc cgctgcacgc tgatcgcgat cgaggacaac
     6541 aagcccgagg cgaccgcggc gatgacgcgc gcgctcgacg cggcgccgct ggcggcaacc
     6601 gagatcgtcg tcattccgac catttacccg gccggcggcg aaaagcagct gatacaggta
     6661 ctaaccggca aggaagtacc gcacggccag ctcgccttcg acatcggtct gctgtgccac
     6721 aatgtcggca cctgcgtggc gatcgcgcgt gcactcgaaa tcggcgaacc gcagatctcg
     6781 cggatcgtca ccgtcagtgg caacaatgtc gagcgccccg gcaactggga agtgcgcttc
     6841 ggcacaccgg tgggccacct gctcgagcag gccggcggct accgccacgg cgaagacgcc
     6901 tggctggtga tgggcggtcc gatgatgggt ttcgcgctgt ccggcgaccg cgtgccgatc
     6961 gtcaaggcca gtaactgcat cctcgcgatg cgcgagcaaa ccatacctca cgcggtcggc
     7021 attcacgacg agtgcattcg ctgcggcaag tgcaccgagg tttgtccggc gcgcctgctg
     7081 ccgcagcaac tctactggca tgcacgcgcc cgcaactacg accgtacccg cgaatttcac
     7141 ctgttcgact gcatcgagtg cggctgttgc tcggcggtct gcccgagcgg cattccgctg
     7201 gtgcaatatt atcgcgccgc caagagcgag atccgcgcgg cgcaaaaggc acagttcaaa
     7261 tccgaccgcg cgcgggtgcg cttcgagttt cgcgaaaagc gcctgttgct gaaaaagcag
     7321 caagacgagg agcgcaagcg gttgaagcgc gaggcgctgc agcaaagacg cgcccgcagt
     7381 gccgaccagg cggcggtgcc cgatccggta caagcggcgc tggaacgcgt caaggagcgc
     7441 cagaaggcca cccaggacga gccgtcgtga gccgcgtact ggtttcctcg ccgtacgttg
     7501 cgccgggcaa ttcgctgcag cggttgatga ttcacgtgct cgtcgcactg ctgcccggaa
     7561 ccctcgccta cagctggcat ttcggccccg gggtgctcgt caacatcgtg ctggcgatcg
     7621 ttttcgcgct cgccttcgag gccggcatac tggcgctgcg caagcggccg gtgatgccgc
     7681 agctgaccga tttcagcgcg gtggtagccg cctggctgtt tgcgctctgc ctgccgatgc
     7741 actcgccgtg gtggctggtg ctggtcggca tgggtttcgc gatggtggcc ggcaaacatc
     7801 tctacggcgg tctcggtttc aacccgttca acccggcgat ggtcggctat gtcgtgttgc
     7861 tgatctcgtt cccgcgggaa atgacgacct ggtatcagcc cggcgcgctc ggcggcgaga
     7921 cgctgggcct cgccgattca ctcgcctaca gcttcggcag cgccgatatc ggccagtggg
     7981 acgcactcag ctcggcgacg ccgctcgatc tggtgagaac cgggctcggg caggacctgc
     8041 cgctatcgga gatacgcaac tcgccgctgt tcggcaactg ggggggcagc ggctgggaat
     8101 ggatcgcact ttgctggggg ctcggcggaa tctacctgtt ggcgacacgc acgattcgct
     8161 ggcagatacc cgtttcgctg ctcgcggcag tgctcgcgat gtcgctggtg tttaatctgc
     8221 tcgatcccga gcactacgcc tcgccgctgt tccacctgct ctcgggcgcg gtgatcatcg
     8281 gcgcgttttt catcgccacc gatccggtca gcgccgcgac cacggacacc ggccgcctct
     8341 gctacggtgc gatgatcggc atcctgatct acgtgattcg cagctggggt ggctaccccg
     8401 acggcgtcgc cttcgcggtg ttgctcgcca atatgtgcgt gccgctgatc gactactaca
     8461 cgcagccccg ggtgtacggc gagcgatgat cgccagcctg catacgatac cgctgctcgc
     8521 cgccgacgcg gctcgagaat accgtggccg accgccgcgg ccaggagttg acgccggcct
     8581 cgcccggacc gcgtcgcgcc ggcgctcggc agcaggagat tgctgaaaca tgcgggccac
     8641 ttaccggcga caaatcctgc tgtcgggcct cttcctgctg ctgttcgcgg tggtcggcac
     8701 ggccctggtg gcgctgaccg agttcgccaa ccgcgacgcg atcgccgcca acgagcggcg
     8761 cgtgctgctg cgcaatctct atgccctgct gcccgccagc gaacacgaca acgatctcgc
     8821 ggaggatacc attcgcctgc cgccggcacc gctgctgggc actgaaggcg cctccacggc
     8881 ttaccgcgcg cgtctcagcg gcgagccggt ggcggtgatc ttcaacagca tcgcgccgga
     8941 cggttacagc ggccgcatcc acctgctggt cggggtttat gtcgacggca gcgtggccgg
     9001 ggtgcgcgtc gtcaagcatg ccgagacccc gggtctcggc gacgcgatcg aagtccgcaa
     9061 gtcgccgtgg atacgcggct tcgacggcaa gtcgctgcaa aaaccacaac tcgaagactg
     9121 ggcggtgaca cgcgacggcg gtgaattcga ccagctgacc ggagccacca tcacgccgcg
     9181 agcggtggtc aacgcggtca gggacacgct gctgtattat cggcgcaacg ctgatatgct
     9241 ttttcagatg aaggccacat catgaccacg cattccgaaa tcacgcgcag cgggctgtgg
     9301 agcaataacg tcgcgctggt gcaactgctt ggcctgtgcc cgctactggc ggtcaccagt
     9361 accgtcgtca acggtctcgg tctcggcatc gccacgctga tcacgctggt gctgtcgaat
     9421 accagcgttt cgctgatccg cgatctggtg cgcaaggaag tgcgtctgcc ggtgttcgta
     9481 ttgattatcg cgtcggtggt cacgatcatc gagctgggca tgaaagccct gttttacgat
     9541 c
//
LOCUS       DATCZV010000511         4653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_465211, whole genome shotgun sequence.
ACCESSION   DATCZV010000511 DATCZV010000000
VERSION     DATCZV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465211"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /gene="hslU"
                     /locus_tag="VIW27_11565"
     CDS             <1..947
                     /gene="hslU"
                     /locus_tag="VIW27_11565"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY5740357.1"
                     /translation="EERVLDALLPRPSEFSEDAATGGDNTRQKFRKMLREGKLDDKEI
                     EVDLQMPSVGVEIMAPPGMEEMTNQLQSMFQNLGGSKNRTRKLRVEDALRMLTDEEAA
                     RLINEEELKLAALENAEQNGIVFIDEIDKVARRKETHGADVSREGVQRDLLPLVEGCT
                     VSTKVGMIKTDHILFIASGAFHVAKPSDLIPELQGRLPIRVELSALTVDDFVRILREP
                     NASLTEQYTALMATEGVRLEFTDDALARIAEVAWNVNEKQENIGARRLHTVMERLLEQ
                     ISFEASEMANQSVTIDAEYVNKHLAELALDDDLSQYIL"
     gene            935..1303
                     /locus_tag="VIW27_11570"
     CDS             935..1303
                     /locus_tag="VIW27_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY5740358.1"
                     /translation="MHTLMKPTEINLHKLSRILELTFDDGSRFELPVEYLRVYSPSAE
                     VQGHGPGQDVLQVGKQDVNIDKIEPIGHYAVKLVFDDNHDSGIYSWDYLYELGSTYGD
                     RWQHYLERLEAAGHKRQPEN"
     gene            1596..2348
                     /gene="ubiE"
                     /locus_tag="VIW27_11575"
     CDS             1596..2348
                     /gene="ubiE"
                     /locus_tag="VIW27_11575"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013577436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY5740359.1"
                     /translation="MQKDQTTDFGFEEIPAADKQRRVGQVFTSVAENYDLMNDVMSFG
                     IHRLWKRFTLEMCALRPGHRVLDLAGGTGDLALAEARRVGSDGRVVLADINPEMLSRG
                     RDRCIDAGLLDNVAAVQCNAEALPFADRSFDCITIAFGLRNVTDKGRALASMQRVLKP
                     GGRLCVLEFSKPLCAPLEKLYDLYSFELLPRLGEWIARDRDSYRYLAESIRRHPDQQA
                     LRQTILADGGFDEVEVHNLSGGIVALHRAFKY"
     gene            2354..3007
                     /locus_tag="VIW27_11580"
     CDS             2354..3007
                     /locus_tag="VIW27_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY5740360.1"
                     /translation="MTADSASTPLLDSIRDSLLAAAEIGGNRLLAFDEAALAGCRELQ
                     GHCIAIHIVDLDLRLYCHPGDWGIRLSSAPPAREADALISGRLMALFNLASQQDKPST
                     SMQERVRFDGDVKLAQRLQRILSGLDIDWEEVLAQLTGDVIAVQLHQRVRRFGDWLKY
                     SADSLLQTTGEYLTEESRATPAPAEFAQFQSRVTVLRNDVARAEARLARLLEQRRGP"
     gene            3007..>4653
                     /gene="ubiB"
                     /locus_tag="VIW27_11585"
     CDS             3007..>4653
                     /gene="ubiB"
                     /locus_tag="VIW27_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY5740361.1"
                     /translation="MRKLFRLLAINFTMARYGLDEVVLSMHFFRPLLLLARINPFNWF
                     RGSHQSRAERLRLAIESLGPIFIKFGQMLSTRRDLFGDEIADELEKLLDRVPPFPLQQ
                     AREIIAQQLGMPLESAFESFDERVLASASIAQVYGARLEGGQDVVVKIVRPGIEQRIR
                     RDIEVMYWLAKMADRYWQEARRVKPLQIVREFETTIYNELDLVREAANASQLRRNFEN
                     SNDLYVPAVHWDYCRPKVMVMERISGIPISDLESLHGRQVNLESLSRKGVEIFFTQVF
                     RHNYFHADMHPGNIFVDPERADDPRYIAVDFGIMGSLSTSDQRYLAENFAAFFNRDYR
                     RVAELHVDSGWVDRDTRVDEFEAAIRSVCEPMFQRPLAEISFGHLLLRLFQTARAFNM
                     EVQPQLLLLEKTFLHIEGIGRQLYPQLDLWTTAKPFIERWLSEQLGVNALLKGLKQNL
                     PYIAEHLPDLPQLAYKALERVANDELQVELKPSQLAAIRAEIRHANRRSIRAIIGGSF
                     VISATLVLALDGLAPIMVGSGQFIVPLLSTVLYLPGLYLLI"
BASE COUNT          908 a         1491 c         1400 g          854 t
ORIGIN      
        1 ccgaggagcg ggtactggat gccctgttac cgcgccccag tgagttcagc gaggatgccg
       61 ccaccggcgg tgacaacacg cggcagaaat ttcgcaagat gctgcgcgag ggcaagctcg
      121 acgacaagga aatcgaggtc gacctgcaga tgccgagcgt cggcgtcgag atcatggcgc
      181 cacccggcat ggaagagatg accaaccagc tgcagagcat gttccagaat ctcggtggtt
      241 cgaagaaccg cacgcgcaag ctgcgcgtcg aggatgcgct gcgcatgttg accgacgagg
      301 aagcggccag gctgatcaac gaggaagaac tcaagctcgc ggcgctggaa aacgccgagc
      361 aaaacggcat cgtattcatc gacgagatcg acaaggtcgc caggcgcaag gaaacccacg
      421 gtgccgacgt ctcccgcgag ggtgtgcaac gcgacctgtt gccgctggtc gaaggctgca
      481 ccgtgtcaac caaggtcggc atgatcaaga ccgaccatat cctgttcatc gcctccggcg
      541 cgtttcacgt cgccaagccc agcgatctga ttccggaact gcagggccgc ctgccgattc
      601 gcgtcgaact gtcggcgctg acggtcgacg atttcgtgcg catcctgcgc gagcccaacg
      661 ccagcctcac cgagcagtac acggcgttga tggcgaccga gggcgtgcgt ctcgagttca
      721 ccgacgacgc gctggcgcgc atcgccgaag tcgcctggaa cgtcaacgaa aagcaggaga
      781 atatcggtgc ccgccgcctg cacacggtga tggagcgcct gctggaacaa atcagtttcg
      841 aagcctcgga gatggccaac cagtcggtga ccatcgatgc cgagtacgtc aacaaacatc
      901 tcgccgagct ggcgctcgac gacgaccttt cgcaatacat actctgatga agccgaccga
      961 gatcaatctg cacaaactgt cccgcattct cgaactcact ttcgacgacg gctcacgctt
     1021 cgagctaccg gtcgagtacc tgcgcgtcta ctcgccgtcc gccgaggtgc aggggcacgg
     1081 acccgggcag gatgtcctgc aggtcggcaa gcaggacgtc aacatcgaca agatcgagcc
     1141 gattggccat tatgcggtca agctggtgtt cgacgataac cacgattccg gcatctacag
     1201 ctgggattac ctctacgagc tcggcagcac ctacggcgat cgctggcaac attacctcga
     1261 acgcctcgaa gccgccggcc acaagcgcca gccggaaaac tgaagggccg acctcgccac
     1321 cgcaaacccc acgatcgggg tcacagccga tttcctccgt tagccgcccg ggaatgtgct
     1381 cgccccgagt tgacgatcgc tgcgattgaa aaggctttcc cgtcttgccg taccgctatt
     1441 cgggttgcga tcatgcaccc ggccgtccag cttcgacaat cccgctcgaa cggagctaca
     1501 tcaagagcct cgaaccgttc aagaaccgca cctcggtgcg ctcatcgcta tttttgccct
     1561 gaccaaaaac ccgcagctat gctatcgttt cgatcatgca gaaagaccag acaaccgact
     1621 tcgggttcga ggagattccg gccgccgaca agcaacggcg tgtcggccag gtatttacct
     1681 cggtagcgga aaactacgac ttgatgaacg acgtgatgtc gttcggcatc catcgcctgt
     1741 ggaagcgctt cacgctcgaa atgtgcgcac tgcggcccgg tcatcgcgtg ctcgacctgg
     1801 ccggcggtac cggcgacctg gcattggccg aagcccggcg tgtcggcagc gacggccggg
     1861 ttgtgctcgc cgacatcaac cccgaaatgc tgagccgcgg gcgcgatcgc tgtattgacg
     1921 ccggcctgct cgacaatgtc gccgcagtgc agtgcaacgc cgaggcgctg ccgttcgccg
     1981 accgctcctt cgactgcatc acgatcgcct tcgggctgcg taatgtcacc gacaagggac
     2041 gcgcgctggc ttcgatgcag cgcgtgttga aacccggcgg ccggctctgc gtgctcgagt
     2101 tttcgaagcc gctctgcgcg ccgctggaaa agctgtacga cctttactct ttcgaactgc
     2161 tgccacgtct cggcgaatgg atcgctcgcg accgcgacag ctaccgctac ctcgccgaat
     2221 ccattcgccg ccaccccgac cagcaagcgc tcaggcagac catcctcgcc gatggcggct
     2281 tcgacgaggt cgaggtacac aacctgagcg gcggcatcgt cgcactgcac cgcgccttca
     2341 agtactgatc gccttgaccg ctgactctgc aagcaccccg ctgctcgatt cgatccgcga
     2401 ttcactgctg gccgcggccg agatcggcgg caaccgcctg ctggcgttcg acgaggctgc
     2461 gctcgccggt tgccgcgagc tgcagggtca ctgcatcgcg atccatatcg tcgacctcga
     2521 tttacggctc tactgtcatc ccggggactg gggcattcgg ctgtcgagcg caccgccggc
     2581 acgcgaggcc gatgcactga tcagcggccg gctgatggcg ctgttcaatc tggcctcaca
     2641 gcaagacaag ccgtcgacat cgatgcagga gcgggtgcgc ttcgacggcg acgtcaagct
     2701 ggcgcaacgg ctgcaacgca tcctcagcgg gctcgatatc gactgggaag aggttctcgc
     2761 tcaactcacc ggcgacgtaa tcgcggtgca gttgcaccag cgggtgcgcc gtttcggcga
     2821 ctggctgaaa tacagcgccg actcgttgct gcaaaccacc ggcgagtacc tgaccgagga
     2881 aagccgggca accccggcgc ctgccgagtt cgcgcagttc cagtcgcggg tcaccgtgct
     2941 gcgcaacgac gttgcgcgcg ccgaggcccg tcttgccagg ctgctcgaac agagacgcgg
     3001 tccgtaatgc gcaaactgtt tcgtctgctc gcgatcaatt tcaccatggc gcgctacggg
     3061 ctcgacgagg tcgtgctatc gatgcatttc tttcgcccgc tgctgctgct ggcgcgcatc
     3121 aatccgttca actggtttcg cggcagccac cagagccgcg ccgagcgcct gcggctggcg
     3181 atcgaatcgc tggggccgat cttcatcaag ttcgggcaaa tgctgtcgac gcggcgcgac
     3241 ctgttcggcg acgagatcgc cgatgaactc gagaaactgc tcgaccgggt accgccgttt
     3301 ccgttgcaac aggcgcgcga gatcatcgcg cagcaactcg gcatgccgct ggagtcggct
     3361 ttcgagagct ttgacgagcg ggtgctggca tcggcgtcga tcgcgcaagt ctacggggca
     3421 cgcctcgagg gcggccagga cgtggtggtc aagatcgttc gccccggcat cgagcagcgc
     3481 attcggcgtg atatcgaggt catgtactgg cttgcgaaaa tggccgaccg ttactggcag
     3541 gaagcgcggc gcgtcaagcc gctgcaaatc gtgcgcgagt tcgagactac catctacaac
     3601 gagctcgacc tggtgcgcga ggcggccaat gccagccagc tgcggcgtaa tttcgagaat
     3661 tcgaacgatc tttacgtgcc cgccgtgcac tgggactatt gccgcccgaa agtcatggtc
     3721 atggagcgca tcagcggcat cccgatatcc gacctggaga gcctccacgg gcgccaggta
     3781 aatctcgaat ccctgtcgcg gaagggtgtg gaaatctttt tcacgcaggt ctttcggcat
     3841 aattattttc atgccgacat gcacccgggt aatatcttcg tcgatcccga gcgcgccgac
     3901 gatccgcgct atatcgccgt cgatttcggc atcatgggat cgctgtcgac ctccgatcaa
     3961 cgctacctgg cggaaaattt cgcggccttc ttcaaccgcg actatcggcg cgtcgccgag
     4021 ctgcacgtgg attccggctg ggtcgatcgc gacactcgtg tcgacgagtt cgaagccgcc
     4081 atccgcagcg tctgcgaacc catgttccag cgaccgctgg ccgagatttc gttcggccac
     4141 ctgctgctgc ggctgtttca gaccgcgcgc gcgttcaaca tggaggtgca gccgcagttg
     4201 ctgctgctcg aaaaaacctt tctgcacatc gagggcatcg gccggcagct ctacccgcag
     4261 ctcgacctgt ggacgacggc caagccgttt atcgagcgct ggctgtcaga gcagctcggc
     4321 gtcaacgctt tattgaaggg tctgaaacag aacctgccgt acatcgccga gcacctgccc
     4381 gatttgccgc agctcgccta caaggcgctt gaacgagtcg ccaacgacga gttgcaggtc
     4441 gagttaaagc catcgcaact ggcggcaatc cgcgctgaaa tccgccatgc caaccggcgc
     4501 agcattcgcg cgataatcgg tggcagtttc gtaatcagtg cgacgctggt gctggcgctg
     4561 gacggcctgg cgccgatcat ggtgggcagc gggcaattca tcgttccgct gctgagtacc
     4621 gtgctttacc tgccgggatt atacctgttg atc
//
LOCUS       DATCZV010000512         3217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_94580, whole genome shotgun sequence.
ACCESSION   DATCZV010000512 DATCZV010000000
VERSION     DATCZV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94580"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /gene="thiL"
                     /locus_tag="VIW27_11590"
     CDS             <1..676
                     /gene="thiL"
                     /locus_tag="VIW27_11590"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY5740362.1"
                     /translation="VDDDWLQDFADGLREAADRFGVQLIGGDTCRGELAVCIQAAGLV
                     PADAYVTRAGAQPGDIVAVSGELGNAALGLADLEAEIELPAAAREKCRLALNRPQPRL
                     ELAPFLSRYATAAIDISDGLVGDLRHLLDASGCGAVIERAAIPVDDWILRRQQYEYAL
                     AAGDDYQICCTLGERDRGEIDAWNRDHPQCRLTVIGAITESGYQLQVGDQAVDLDGQR
                     GFRHFD"
     gene            669..1145
                     /locus_tag="VIW27_11595"
     CDS             669..1145
                     /locus_tag="VIW27_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY5740363.1"
                     /translation="MTRIPPRLLFNPAHCLSLGFGAGLSPIAPGTAGTLVAVPLYLAL
                     AQLAWPWYLAIVVIGFAAGIWLCGYTSKALGEHDHGGIVWDEIVGFWITMIAVPPTWQ
                     WILLGFVLFRVFDILKPWPVRIADRRIPGGLGIMFDDLLAGLYALAGVQLALRLLD"
     gene            1168..1791
                     /locus_tag="VIW27_11600"
     CDS             1168..1791
                     /locus_tag="VIW27_11600"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY5740364.1"
                     /translation="MTAILLALTLGVAAADGDVKVIALFAGKALLQVGEQRKIVADGE
                     TFAGVTLKSATGRGAVVVIDGEEIEVGLNRSIGGTYKKRDNASLKIYPDSIGMYYADG
                     KINGRATRFLVDTGATYVTMSGRFARSLDIDYTRGRQSAAQTASAIVPVWQIQLDSVS
                     VGGITVRNVTATVIVGDKPFDVLLGNSFLEHTHMQKAGSVLELKKRF"
     gene            2043..>3217
                     /gene="gltX"
                     /locus_tag="VIW27_11605"
     CDS             2043..>3217
                     /gene="gltX"
                     /locus_tag="VIW27_11605"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008910632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY5740365.1"
                     /translation="MIKTRFAPSPTGVLHVGGARTALFCWLLARKVSGKFVLRIEDTD
                     LERSTPESVQAILDGMAWLGLDCDEGPFYQTRRFDRYREVVEQLISQGDAYYCQCSRE
                     RLDALREKQMAERQKPRYDGCCRGLGLRPSADSPMVVRFCNPQQGVVSFYDHVKGEIT
                     VDNRELDDLIIARGDGSPTYNLSVVVDDMDMEITHVLRGDDHVNNTPRQINILRALGA
                     PIPEYAHVPMILGDDGKRLSKRHGAVGVMEYHEAGYLPQAMLNYLVRLGWSYGDQEIF
                     SREEMVEHFDLDSLNRSASSFNTEKLNWVNQHYLMHEPLADIVELVRQRLERLQIEYH
                     RDFDLDAVVDLYRQRAVTINALVDAIEYFFRDFDDYDSRAAAKVLKPAAREPLEHAR"
BASE COUNT          597 a          973 c         1013 g          634 t
ORIGIN      
        1 agtcgacgac gactggttgc aggatttcgc cgatggtctg cgcgaggccg cggatcgatt
       61 cggcgtgcaa ctcatcggcg gcgatacctg tcgcggtgaa ctggccgtct gtattcaggc
      121 agccgggctg gtgcccgccg atgcttacgt gacccgcgcc ggcgcgcagc cgggcgatat
      181 cgtcgcggtc agcggcgaac tcggcaacgc cgcgctcgga ctggccgacc tcgaggccga
      241 gatcgagctg cccgcggcag cccgtgaaaa atgccgtctg gcgttgaatc gcccgcaacc
      301 gcgcctcgag ctcgcacctt tcctgagtcg ttacgcgacc gcggcaatcg atatttccga
      361 cggcctggtc ggcgacctgc gccacctgct cgatgccagc ggttgcggtg cggtcatcga
      421 gcgcgcggcg attccggtcg atgactggat cctgcgccgc cagcaatacg aatatgcgct
      481 cgccgccggt gacgattacc agatatgttg cacgctcggc gagcgggacc gcggcgaaat
      541 cgacgcgtgg aaccgtgacc acccgcaatg tcggctgacc gtaatcggcg ccattaccga
      601 atccggatac cagttgcagg tcggtgatca ggcggtcgat ctggacgggc agcggggatt
      661 tcggcacttt gactaggatt cctccacgct tgctgttcaa cccggcgcat tgcctgtcgc
      721 tcggtttcgg cgccgggctg tcgccgatcg cgccgggtac cgccggtacg ctggtcgcgg
      781 tgccgttgta cctggcgctc gcccagctgg cctggccctg gtacctggcg atcgtggtaa
      841 tcgggtttgc tgcgggtatc tggttgtgcg gttatactag caaggcactc ggtgagcacg
      901 accacggcgg catcgtctgg gacgagatcg tcggcttctg gatcacgatg atcgcggtac
      961 cgccgacatg gcaatggatt ctgcttggat ttgtgctatt tagagtattc gatattctca
     1021 aaccctggcc ggtcaggatt gcggaccgca ggataccggg agggctggga atcatgttcg
     1081 acgatttgct ggcggggctc tatgcgctgg ccggcgtgca actcgcgttg cggttactgg
     1141 actagattta tggacgcagg gaaatacttg accgcgatac ttctcgccct gaccctcggt
     1201 gtcgccgctg ccgacggcga tgtcaaggtc atcgcgttgt ttgccggcaa ggcactgctg
     1261 caggttggtg agcagcgcaa gatcgtcgcc gacggcgaaa ccttcgcagg cgtgacactg
     1321 aaatccgcca ccggccgcgg tgcggtggtg gtcatcgacg gcgaggagat agaggtcggc
     1381 ctgaaccgct caatcggcgg aacctacaag aagcgcgata acgcatcgct gaagatttac
     1441 ccggactcga tcggcatgta ctacgccgac ggcaagatca acgggcgtgc gacccgtttc
     1501 ctggtcgata ccggcgcgac ctacgtgacc atgagcgggc gcttcgcgcg cagtctcgat
     1561 atcgattata ctcgcggcag gcagagtgcc gcacaaaccg cgtcggcgat cgtaccggtg
     1621 tggcagatcc agctcgacag cgtcagtgtc ggcggcatca cggtgcgcaa tgtcaccgct
     1681 accgtgatcg tcggcgacaa accctttgac gtgctgctgg gaaattcgtt cctggaacac
     1741 actcacatgc agaaagcggg ctcggtgctc gaactcaaga aacgttttta gcccgcctac
     1801 ctcaccatta tcctgcacgg acgccgcaaa gctcaccgtg acgcgctgta ttgccaccgg
     1861 tccgcttgac ctggcgtcgg cgaggccagc gcggacctct gctgcgtgca gcgcgccaca
     1921 acgtctttgc gatgcaccgg cgacgtaaat cggtgcaata tgcgggctag cgaaatattt
     1981 tatcggccgc ggcagcgata ttccgttaca ctagcgccgg ttcgttttca tcatcaaatt
     2041 caatgatcaa gacacgattc gcaccgagtc ccaccggtgt attgcatgtc ggcggcgcca
     2101 gaacggcgct gttttgctgg ttgctggccc gcaaagtgag cggcaagttc gtattacgca
     2161 tcgaagatac cgatctcgag cgctcgacac ccgagtcggt gcaggcgatc ctcgacggca
     2221 tggcctggct ggggctcgac tgcgacgagg gcccgttcta ccagacgcgc cgtttcgacc
     2281 gctatcgcga ggtcgtcgag caactgattt cgcagggcga tgcctactat tgccagtgtt
     2341 cgcgcgagcg cctcgatgca ctgcgtgaaa agcagatggc cgagcggcag aaaccccgct
     2401 acgacggttg ttgccggggc ctcggtctgc gaccgagcgc ggactcgccg atggtggtgc
     2461 gcttctgcaa cccgcagcaa ggggtggtca gtttttacga tcatgtcaag ggcgagatca
     2521 cggtcgataa tcgcgaactc gacgacctga tcattgcgcg cggcgacggc agtccgacct
     2581 ataacctgtc ggtggtggtc gatgacatgg atatggagat tacccacgtg ctgcgcggcg
     2641 acgatcacgt caacaatacc ccgcgccaaa tcaatatcct gcgcgcgcta ggggcgccga
     2701 tccccgagta tgcccacgta ccgatgattc tcggcgacga cggcaagcgt ttgtcgaaac
     2761 gccacggtgc ggtaggcgtc atggagtacc acgaggcggg ctacctgccg caggcaatgt
     2821 tgaactacct ggtgcgtctc ggctggtcgt acggcgacca ggaaatcttc agtcgcgagg
     2881 aaatggtcga gcattttgac ctcgattcgc tgaaccgctc ggcgtcctcg ttcaataccg
     2941 agaagcttaa ctgggtcaac cagcattacc tgatgcacga accgctggcg gacatcgtcg
     3001 agctggtcag gcagcggctc gagcggctgc aaatcgaata ccacagagat ttcgatctcg
     3061 acgcggtcgt cgatctctac cgccagcgcg cggtcaccat caatgcgctg gtcgacgcga
     3121 tcgagtattt tttccgcgac ttcgacgact acgattcgcg tgctgccgcc aaggtgctga
     3181 agccggccgc gcgcgaaccg ctcgagcacg cccgcga
//
LOCUS       DATCZV010000513         3608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_42802, whole genome shotgun sequence.
ACCESSION   DATCZV010000513 DATCZV010000000
VERSION     DATCZV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42802"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VIW27_11610"
     CDS             <1..1315
                     /locus_tag="VIW27_11610"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY5740366.1"
                     /translation="IHPYLARREGLEPVTYPDARLKPILQRTLGIAIFQEQAMRIAIA
                     VGNFSAGEANELRKKIGAWGIKDFNRDLHPLLTRLEQGMRENGVKPEFAEQILGQMKG
                     FAEYGFPESHAVSFSLIAYASSYLKCHFPAAFYISVLNSQPMGFYSPHALLQAARREN
                     IRLLPISLNHSDWDHRLEKPAGEADYAIRLGFRLVRGLAAAIADEIVRRREVSGGWRD
                     FAAFIGDCSLARDAITALAAAGVFGEFGDARPDALWQAEAVPVYPLLDNVDDRVDWPA
                     ESRLDAIRKDFRAFRTSLGAHPAAVLKQEQWPYPVAVEKICSAASLAESAADSDVFAF
                     GMVLVKQSPGSAKGMVFVTLEDETGFVNLALTPQVYARYYRLVDQQPFLCVVGKLQRV
                     NQSHSILVKRVFEQGDGARLLRLPGRRDPAPGSAVAELLKPRAFR"
     gene            complement(1343..1984)
                     /locus_tag="VIW27_11615"
     CDS             complement(1343..1984)
                     /locus_tag="VIW27_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5740367.1"
                     /translation="MIQLLIQQQYLLFVFIACALIFSLTFHEYGHGKMASLIGDDTAE
                     RAGRLTLNPLPHIDPVGLLMVFLIGIGYAKPVPANPRKFNTRWGTLLVAAAGPGMNLL
                     LAVVLINLYGLGLKSGIGLFESWGANFFFTYLALINMLLMLFNLLPVGPLDGSYILPY
                     VLPPDLARRYQHYNQRFGAFALLALIGLHFLGVPIFEVIWDVGGYLLDLIRVF"
     gene            2495..>3608
                     /locus_tag="VIW27_11620"
     CDS             2495..>3608
                     /locus_tag="VIW27_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740368.1"
                     /translation="MSQDLINRYQNSHRLQFQSQETEASYRESQQSRVRKSSGYAFGV
                     LLVLSLIFTYLEYRAFGHDVPIPMYGYSLAALLALCNLLLNLLSTGTYFQAMRLVANG
                     VLAMGTVIGAVYLQKYSAYHVIEFVLLAVWLGSLRAVRMVFTLAVNLLLASLFVAVMF
                     VTDISGFWMTLVGVLLIAAVLLAAFIAYLSERAQRQLFLLSEFNDSMINRQELWAFTL
                     IDLDMALDGIRDFKEVIGLLKGYLEPVIDFDSYILTSLEGQGPKPVPDKIEGTLFETE
                     DSTLWSEDLLGKLTQTRQATTSAEHEIVKGPLGISRKRFTSFRLDVPVFKDSKLMGVI
                     SLRRASQPFDELDQVASVSIAAQAMLIFKRSIGSPAA"
BASE COUNT          754 a         1055 c         1076 g          723 t
ORIGIN      
        1 gattcatccc tatctcgcgc ggcgcgaggg actcgagccg gtgacttatc ccgacgcgcg
       61 gctgaagccg attttgcagc gcacgctggg catcgcgatc ttccaggagc aggcgatgag
      121 gatcgcgatc gcggtgggca atttcagcgc cggcgaggcc aacgaattgc gcaagaaaat
      181 cggtgcctgg ggaatcaagg atttcaaccg cgacctgcac ccgctgctga cccggctcga
      241 gcagggcatg cgcgagaacg gcgtcaagcc cgagttcgcc gagcagattc tcgggcagat
      301 gaagggcttt gccgagtacg gctttcccga gtcgcacgcg gtatcctttt cgctgattgc
      361 ctatgcatcg agctacctga aatgccattt tccggccgcc ttctatatct cggtgctcaa
      421 ttcccagccg atgggttttt actcgccgca tgcgctgctg caggcggccc ggcgagaaaa
      481 cattcggtta ttgccgatct cgctgaacca ctcggactgg gatcaccggc tcgaaaagcc
      541 ggccggtgaa gcggattacg cgattcgtct aggctttcga ctggttcgcg ggctcgccgc
      601 agccatcgcc gacgaaatcg ttcgccgccg cgaggtgagt ggcggttggc gggattttgc
      661 ggctttcatc ggcgactgca gcctggcgcg cgacgcaatc accgcgctgg cggcggctgg
      721 tgtattcggc gaattcggcg atgctcgccc ggacgcgctt tggcaggcag aggcggtgcc
      781 ggtctacccg ttgctggata atgtcgacga tcgtgtcgac tggccggcgg aaagccgtct
      841 cgacgccatc cgcaaggatt tcagggcttt tcgcaccagc ctcggcgcgc acccggcagc
      901 ggttctcaag caggagcagt ggccctaccc ggtggcggtc gaaaaaatct gttcggccgc
      961 gagccttgct gaatcagccg ccgatagcga cgtctttgca ttcggtatgg tgctggtcaa
     1021 gcagtcaccg ggctcggcga aaggcatggt cttcgtcacc ctcgaggatg aaaccgggtt
     1081 cgtcaatctg gcgctgaccc cgcaggtcta tgcacgctat tatcgcctgg tcgatcaaca
     1141 gccgttcctg tgtgtggtcg gcaagctgca acgcgtcaac cagtcccatt cgatcctggt
     1201 caagcgggta ttcgagcagg gcgacggcgc gcgcctgctg cgcctgcccg gcaggcgcga
     1261 cccggcaccc ggatcggcgg tggccgaact gctcaagccg cgcgcgttcc gctagtagcc
     1321 ggcttgcgac gcaatcgtta actcagaaga cgcggatcaa gtcgagcagg taaccgccga
     1381 cgtcccagat cacctcgaag atcggcacgc ccaaaaaatg cagcccgatc aatgccagca
     1441 gcgcgaatgc cccgaagcgc tggttgtagt gctggtaccg ccgcgcgagg tctggcggca
     1501 gtacgtaggg caggatgtag ctgccgtcga gcgggcccac cggcaacagg ttgaacagca
     1561 tcaacagcat gttgatcaat gccaggtagg taaaaaagaa attcgccccc cagctctcga
     1621 acagcccgat tcccgatttc aggccgaggc cgtagaggtt gatcagcact actgccagca
     1681 gcaggttcat ccccgggccg gccgccgcca ccagcagtgt tccccagcgg gtattgaact
     1741 tgcgcgggtt ggccggtacc ggcttggcat aaccgatacc gatcaggaaa accatcagca
     1801 agccgaccgg atcgatatgc ggaagcgggt tcagggtcag cctgccggcg cgctccgcgg
     1861 tatcgtcgcc gattagtgat gccatcttgc catgaccgta ttcgtgaaag gtcagcgaga
     1921 aaatcagtgc acaggcaatg aacacgaaca gcaggtactg ttgttggatc aggagctgaa
     1981 tcatcggcag ggatcgatcg ccattacgga aaaggctgca ctttaccgct aacctcgcgt
     2041 gatcgcagcc ccctcgacaa gaaaaaaacc aaatgccggc atatcgagca cgaacccttg
     2101 ccgactaccg gcgggatgac gtcgaaaacg gtcgcgccgc gaaacccggc aattggcggg
     2161 caatcgccct tgccgccgcc gcttgcgcgc tgttccgcgc cgcttgccga gagcacgaat
     2221 atgattaagt gaaaaattaa caaattcaca taagcccatg aaaattataa atatctacta
     2281 taatcttcga tgagccgcca ggattcgtac gcattccgta ataaaaccgc cgataccggg
     2341 tttaccggtt ggtcgaagac gcgtctgagc gggaagtgaa aaatagtcta tacataggac
     2401 aaaaccacga tactaaatct cgcggagccg atttcgatgt accgcgaccg atatccagaa
     2461 tgcagcaatt gacgacaggt aaagggtaac cagtgtgtct caagacctga taaacaggta
     2521 ccagaattcc caccgacttc agttccagag tcaagaaacc gaggccagtt accgggagtc
     2581 gcaacaatcg cgcgtgcgca agtcgagcgg ctatgcattc ggcgtattgc tggttttatc
     2641 cctgattttc acctatctcg agtatcgcgc gttcgggcac gatgtgccga tacccatgta
     2701 cggctattca ctggcggcgt tgctggccct gtgcaacctg ctgctcaacc tgctgtcgac
     2761 gggcacttac ttccaggcga tgcgactggt cgccaacggt gtattggcga tgggtaccgt
     2821 gatcggcgcg gtatacctgc aaaagtacag tgcataccat gtcatcgagt tcgtgctgct
     2881 ggcggtatgg ctcggcagtc tcagggctgt caggatggtg ttcacgctgg ctgtcaacct
     2941 gctgctcgcg agcctgttcg tcgccgtcat gttcgttacc gatatctcgg gcttctggat
     3001 gacactggtc ggcgtgctgt tgattgccgc ggttctgctg gcggcattca ttgcttacct
     3061 gagcgaacgt gcgcagcgtc aactgttcct gctttcggag ttcaacgaca gcatgataaa
     3121 ccgccaggag ctgtgggcat tcacgctcat cgatctcgac atggcgctcg acggtattcg
     3181 cgatttcaag gaagtgatcg gcttgctgaa gggatatctg gagccagtga tcgattttga
     3241 ctcgtatatc ctcacctcgc tggaaggtca ggggcccaaa ccggtgccgg acaagatcga
     3301 aggtacgctg ttcgaaaccg aggacagcac gctgtggtcg gaagatttgc tcggcaagct
     3361 gacgcagacg cgccaggcaa ccaccagcgc cgagcacgaa atcgtcaagg gtccgctggg
     3421 catcagcagg aaacgcttta cgtccttccg cctcgacgtg cccgtgttca aggattcaaa
     3481 gctgatgggc gttatcagcc tgcggcgcgc gtcgcagccc ttcgacgaac tggaccaggt
     3541 tgccagcgtc agtattgccg cgcaggcgat gctgatcttc aagcgctcga tcggcagccc
     3601 ggctgccc
//
LOCUS       DATCZV010000514         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_518115, whole genome shotgun sequence.
ACCESSION   DATCZV010000514 DATCZV010000000
VERSION     DATCZV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518115"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VIW27_11625"
     CDS             complement(<1..572)
                     /locus_tag="VIW27_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5740369.1"
                     /translation="MELIRNFSIIAHIDHGKSTLADRFIQICGGLSEREMEAQVLDSM
                     DLERERGITIKAQSVSLNYQALDGKTYTFNIIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQSVANCYTAIDLDLEVIPVLNKIDLPAADSQRVKQEIENVIGLDTAETIE
                     VSAKTGIGVEDLLEQIIEKIPSPKGSEDNPT"
     gene            complement(668..907)
                     /locus_tag="VIW27_11630"
     CDS             complement(668..907)
                     /locus_tag="VIW27_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5740370.1"
                     /translation="MELTLYYREDCHLCDTMRKALVAFSRKQHPLSWREIDIDRDPEL
                     IRRYDSRVPVLCRGESEICHFFFDETALRETLALA"
     gene            complement(920..2335)
                     /locus_tag="VIW27_11635"
     CDS             complement(920..2335)
                     /locus_tag="VIW27_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY5740371.1"
                     /translation="MKSSINFLVRTLLVLTVCSPLVARADLPDFTAIVVEAQNSVVNI
                     STKVRVKPSSSKQYEIPELPEGSPFGELFEKFFDRDEFERRHRDAQSLGSGFIISDDG
                     YILTNHHVIAGADEVIVRMVNRKEFTARVIGSDEASDVAVLKIDSEDLPTLKIGDSDD
                     LKVGEWVLAIGSPFGFDASVTAGIVSAKGRSLPSDNYVPFIQTDVAINPGNSGGPLFN
                     LDGEVVGINSQIYSRSGGFMGLSFAIPIEMAVDVANQIKETGAVSRGWLGVLIQEVTR
                     ELAESFGMENPHGALVAKVLDDSPAAEAGLQVGDVIVEFNGRKVMRSSALPPLVGRAK
                     VGKEAQLTVIRDKRRRDIGVLIAQLPTTITQAAFQPPADEPQEESALGMKVEALGSEA
                     RKRLKIESGVQVVEVDETGPALEAGIQEGDVITMIDNREIESVDDFEKVTGELKSGKS
                     VALLVQRRAGPVFLAIRPEDS"
BASE COUNT          459 a          739 c          702 g          487 t
ORIGIN      
        1 gtcggattgt cttccgagcc cttgggcgac ggaatctttt cgatgatctg ttccagcagg
       61 tcttcgacac cgataccggt cttggcgctg acctcgatcg tttccgcggt atcgaggccg
      121 atgacattct cgatttcctg tttcacccgc tgcgaatcgg cggccggcaa atcgatcttg
      181 ttgagcaccg ggatcacttc caggtcgagg tcgatcgcgg tatagcaatt ggcgacgctc
      241 tgcgcctcca cgccctgcga ggcgtcgacc accagcagcg cgccctcgca ggctgccagc
      301 gagcgcgaga cttcgtagga aaagtcgacg tggcccggcg tatcgatgat attgaaggta
      361 taggtcttgc cgtcgagggc ctggtagttg agcgacacgc tctgcgcctt gatggtgatg
      421 ccgcgctcgc gctcgagatc catcgagtcg agtacctgcg cctccatttc acgttcgctg
      481 aggcccccgc agatctggat gaaacggtcc gccagggtcg atttgccatg atcgatatgg
      541 gcaataatcg aaaaattgcg aattaactcc atgcgcattc tggtttgtgc cctgcccggc
      601 ggtgaattga ctgcgtgtga cgccgacgct gcggaagccg gcactggatg cttggaaccc
      661 gcgcagttta cgctaaagcc agcgtttccc gcaacgccgt ttcgtcgaaa aagaaatggc
      721 agatttcgct ctctccgcgg cacagcaccg gaacgcgcga atcgtagcgg cggatcagct
      781 cgggatcgcg gtcgatatcg atttcccgcc agctaagcgg gtgttgtttg cgcgagaatg
      841 ccaccagcgc cttgcgcatc gtgtcgcaca ggtggcagtc ctcgcggtag tagagcgtca
      901 gttccaagcc gcgcgaaaat caggaatctt ccgggcgaat ggcaagaaat accgggccgg
      961 cacgacgctg caccagcagc gccaccgatt tacccgactt caactcgccg gtcactttct
     1021 cgaaatcgtc gaccgactcg atctcgcgat tgtcgatcat cgtgatcacg tcgccttcct
     1081 gaatcccggc ctccagcgcg gggccggttt cgtcgacctc cacgacctgc acaccggatt
     1141 cgatcttcag gcgcttgcgg gcctcgctgc cgagcgcctc gaccttcata ccgagcgccg
     1201 attcttcctg cggctcgtcg gctggcggct ggaacgcggc ctgcgtgatg gtggtcggca
     1261 actgcgcgat caacacgccg atatcacggc gtcgcttgtc acgaatcacc gtcaactggg
     1321 cttccttacc gaccttggca cgaccgacca gcggcggcag cgcactggag cgcatcactt
     1381 tcctgccgtt gaattcgacg atcacgtcgc cgacctgcag accggcctcg gcggccgggc
     1441 tgtcgtcaag caccttggcg accagcgcgc cgtgcgggtt ttccatgccg aaggactccg
     1501 ccagctcgcg ggtcacttcc tggatcagca cgccgagcca gccgcgcgac accgcgccgg
     1561 tttccttgat ctggttggcg acgtcgaccg ccatttcgat cgggatcgca aacgacagcc
     1621 ccatgaaacc acccgagcgg ctgtagatct gtgaattgat gccgaccacc tcgccatcga
     1681 ggttgaacag cggaccgccc gagttgccgg gattgatcgc gacgtcggtc tggatgaacg
     1741 gcacatagtt gtcgctcggc aggctgcggc ccttggcgct gacaataccg gcagtgaccg
     1801 aggcatcgaa accgaacggc gaaccgattg ccagcaccca ctcgccgacc ttgaggtcgt
     1861 cagaatcgcc gattttcaga gtcggcaggt cctcggagtc gatcttcagc accgcgacgt
     1921 cggaggcctc gtccgaaccg attacccgcg cggtgaattc cttgcggttg accatgcgga
     1981 cgatgacttc gtcggcaccg gcgatgacat ggtgattggt cagaatatag ccgtcgtcgg
     2041 agatgatgaa gcccgaaccc agcgactgcg catcgcggtg ccggcgctcg aattcgtcac
     2101 ggtcgaaaaa tttctcgaac agttcgccga acggcgatcc ctccggcagt tccggaattt
     2161 catattgctt cgacgaactg ggcttgaccc gcaccttggt gctgatgttg acgaccgagt
     2221 tctgggcctc gacgacgatt gcggtgaaat cgggtaaatc cgcgcgcgcc accagcggcg
     2281 agcagacggt cagcaccaac agggtcctga cgagaaaatt gattgaagat ttcatgaata
     2341 aaataccgtt tgcttgaatt taaacacggg gattggtgtc aatcccc
//
LOCUS       DATCZV010000515         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_412582, whole genome shotgun sequence.
ACCESSION   DATCZV010000515 DATCZV010000000
VERSION     DATCZV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412582"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VIW27_11640"
     CDS             <1..602
                     /locus_tag="VIW27_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5740372.1"
                     /translation="RPVRIDNDANCFALSEALGGAARGYRVVFGVIVGTGTGGGIVAD
                     GRLLRGAHAIGGEWGHNPLPWRRAEDGEPSCYCGKAACIETFLSGPGMARGYRSRFGG
                     ELSSRQIVAGAAAGEQRCARMLDAYHDQMARALSHLINILDPDAIVLGGGMSNIDSIY
                     VELPRRLPEYVFSDFVATPILRAEHGDASGVFGAALLWD"
     gene            complement(620..1969)
                     /gene="mgtE"
                     /locus_tag="VIW27_11645"
     CDS             complement(620..1969)
                     /gene="mgtE"
                     /locus_tag="VIW27_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY5740373.1"
                     /translation="MVEEDIEQLRQDISLALDAEDQAELSKLLAGIGVAETARLLDSL
                     PSQQRDSIWPLLAPEELGAVLLETQDDVSDARLKQLEPEEIAAIVEIMPDVDDQADII
                     LALPTDKLVRTLHTLDAHKRERLNAVLAYSEDTAGGLMNIDQVTIRADVSLDVVLRYL
                     RMRGELPTHTDQLFVTDRRDHYLGALYLSDLLTNDPEVRVSDIMRTDVDAMHAHMSDT
                     EVAREFEHYDLVSAPVVDEEHKLIGRITVDDVVDVIRDEGEHSLMSMAGLSEEDDMFS
                     PVMVTSRRRGIWLGINLLTAFLAAWVIGFFEDILDQVVALAILITIVPSMGDIAGSQT
                     LTIAIRGLAVGQLGSSNYRSLLKKEVLVGLLNGLIWALVVATLSVLWFGNYSIGAIIA
                     FALVVNLIAGALAGAVIPMVLRQIGIDPAIAGGVVLTTVTDVVGIIAFLGVATLVLG"
     gene            complement(1976..2245)
                     /locus_tag="VIW27_11650"
     CDS             complement(1976..2245)
                     /locus_tag="VIW27_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY5740374.1"
                     /translation="MISETVIIINKLGLHARAASKLVNCAGQFESEVFLSRKDNRVNA
                     KSIMGVMMLAASKGVELGLEVDGRDEQACRDALVALIGNRFGEAE"
BASE COUNT          411 a          781 c          841 g          452 t
ORIGIN      
        1 agcgaccggt gcgcatcgac aacgatgcca attgcttcgc gctgtccgag gcgctgggcg
       61 gtgcggcgcg cggttaccgg gtcgtattcg gggtcatcgt cggcaccggt accggcggcg
      121 gtatcgtcgc cgatggcaga ctgctgcggg gggcgcatgc gatcggcggg gaatggggcc
      181 acaatccgct gccgtggcgg cgcgccgagg acggcgagcc gtcctgctac tgtggcaagg
      241 ccgcctgcat cgagaccttt ctctcggggc cgggaatggc gcgcggttac aggtcccgct
      301 ttggcggcga actgtcgagt cggcagatcg tcgccggcgc cgcggcgggt gagcagcgct
      361 gcgcgcgaat gctcgatgct taccacgacc agatggcgcg agcgctgtcc catttgatca
      421 acatcctcga tcccgacgct atcgtgctcg gcggcggcat gtcgaatatc gactcgatat
      481 acgtcgaact gccgcggcgc ctgcccgagt acgtgttttc ggacttcgtc gccacaccga
      541 tcctgcgcgc cgagcacggc gatgccagcg gtgtgttcgg cgccgcgctg ttgtgggact
      601 gatcgccgtc gctcgtcgtt cagccgagca ccagcgtggc gacgccgaga aaggcgatga
      661 taccgaccac gtcggtcacc gtggtcaata ccacgccgcc ggcgatcgcc gggtcgatgc
      721 cgatctggcg cagcaccatc gggatcaccg cgccggccag cgcgccggcg atcaggttga
      781 ccaccagcgc gaaagcgatg atcgcaccga tactgtaatt gccgaaccag aggaccgaga
      841 gggtcgccac caccagcgcc cagatcagcc cgttcagcag gccgaccagg acttcctttt
      901 tcagcagcga gcggtagttg gagctgccca gctggcccac cgccagtccg cgaatcgcga
      961 tggtcagggt ttgcgagccg gcgatgtcgc ccatgctggg gacgatggta atcagaatcg
     1021 cgagcgcgac cacctggtcg agtatgtcct cgaagaagcc gatcacccag gcggcgagga
     1081 aggcggtcag caggttgatg ccgagccaga tgccgcgacg ccgcgaggtt accattaccg
     1141 gtgaaaacat gtcgtcttcc tcggaaagac cggccatgct catcagcgaa tgttcgcctt
     1201 cgtcacgaat cacgtcgacc acgtcatcca ccgtgatgcg accgatcaac ttgtgttcct
     1261 cgtcgaccac cggcgcgctg accaggtcgt aatgctcgaa ttcgcgcgcc acctcggtat
     1321 ccgacatgtg cgcgtgcatc gcgtcgacgt cggtgcgcat gatatcgctg acgcggactt
     1381 ccgggtcgtt ggtgagcaga tcactgaggt ataacgcgcc gaggtaatgg tcgcggcggt
     1441 cggtgacgaa cagctggtcg gtatgggtgg gcagttcgcc gcgcatgcgc aggtagcgca
     1501 gcaccacgtc gagggacacg tcggcgcgga tcgtcacctg gtcgatgttc atcaggccgc
     1561 cggccgtatc ctcgctgtag gcaagcaccg cgttgagccg ctcgcgcttg tgcgcgtcca
     1621 gcgtgtgcag cgtgcgcacc agcttgtcgg tgggcagggc gaggatgatg tcggcctggt
     1681 cgtcgacatc gggcatgatc tcgacgatgg cggcgatttc ttccggctcg agttgcttca
     1741 ggcgtgcgtc gctgacgtcg tcctgagttt ccagcagcac cgcaccgagt tcctccgggg
     1801 ccagcagcgg ccagatcgag tcgcgctgct gggacggcag cgaatcgagc aggcgcgcgg
     1861 tttccgcgac gccgatgccg gccagcagct tggacagctc ggcctggtcc tcggcatcca
     1921 gcgccagcga gatatcctgg cgcagttgct cgatgtcttc ctcgaccatg cgcgcctact
     1981 cggcttcgcc gaagcggttg ccgatcagtg cgaccagtgc atcgcggcag gcctgctcgt
     2041 cgcgcccgtc gacctcgaga ccgagttcga ctcccttgct ggcggcgagc atcatgaccc
     2101 ccatgatgct cttggcattg acgcggttgt ccttgcggct gagaaagacc tcgctttcga
     2161 actggcccgc gcagttgacc agcttggaag ccgcgcgtgc gtgcagtccg agcttgttga
     2221 tgatgatgac ggtttcggaa atcatgtcca gtcccgtgtt cccccgctgc cgccggttgc
     2281 tcgaatccgg gcaagcggcc agggatgcaa atgcgcgtag tttaatacag gcggcggtaa
     2341 cggcaagcgg cggtgggcgg caggggtctt cgcgcgccag cgcgaaacca cctcagggca
     2401 ggtcgcggtg gcgggtggaa acctgctgca gctgcttttt gaggcccgag gcgatgcgtt
     2461 ccgcgagata gaccgagcgg tgctg
//
LOCUS       DATCZV010000516        10263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_625165, whole genome shotgun sequence.
ACCESSION   DATCZV010000516 DATCZV010000000
VERSION     DATCZV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625165"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VIW27_11655"
     CDS             <1..358
                     /locus_tag="VIW27_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5740375.1"
                     /translation="VIDNVAGDGFPELLKLMRRGGRYASSGAIAGPLVNLDMRDFYLS
                     DLNLIGCTAWDEPVFPNLVGYIERGEIQPLLAKTYALDEIAVAQQEFLKKNHIGNFVL
                     IPPPPKLGSESFFIQI"
     gene            complement(473..1402)
                     /locus_tag="VIW27_11660"
     CDS             complement(473..1402)
                     /locus_tag="VIW27_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5740376.1"
                     /translation="MIATLNAIAPIFLIIITGYLLSVSRVVGESVWSAIEHLCFYLLF
                     PFLIIRTLSRADLGDLPILDFMTVLIVAILGMSALLILIQALVWRRHPESGPSFTSIF
                     QGATRFHGFVAIAIIGPLYGDDGVTLGALALSIMVPLLNTITVIVLSIYGRSDTRPEF
                     AAVGRKILTNPLILACGAGLAFNWLGIPDILFSTIDIIGDGGLGLALLAVGAGMRFGH
                     AAQHKMLLAIGVLTRLIGMPAIVIAMSWLVGLEGLPRTVAIIAGAVPTASSAYVMARK
                     MGGDAALMSSIVTFQVIAAFFTLPLFIYIADHL"
     gene            complement(1403..1633)
                     /locus_tag="VIW27_11665"
     CDS             complement(1403..1633)
                     /locus_tag="VIW27_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740377.1"
                     /translation="MQTGDRVYYRYTDSSGNQIKYAAVVLACRDDGIVIRIGRYDPGT
                     QDIKTFESSVSADSLQPRSIPCSYEAILAGER"
     gene            complement(1633..2523)
                     /locus_tag="VIW27_11670"
     CDS             complement(1633..2523)
                     /locus_tag="VIW27_11670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740378.1"
                     /translation="MALPIYATPGQRIWHYFYMAFCTFVLFFLVAPLVAVIPLSFTSS
                     PFLNFTPEMLALDPDAFSVRWYKSLVGICTDVDDAASTMCHDKWMTGAGRSLYYAVIS
                     TLLATFLGTLAALGLSSEHMPFKRAIMALMISPLIVPLIITAAGMAFFFADVNLSNTD
                     AGIILAHTILGLPFVVITVTSTLVGFDRGLMRAAANLGGTPTYNFFKIQMPLIAPGMI
                     SGALFAFITSFDEIVIILFIGGPDQHTLPRQMWSGIRQEISPTILSAATILVLFSIIL
                     LTTLEMLRRRSERLRGITPH"
     gene            complement(2528..3787)
                     /locus_tag="VIW27_11675"
     CDS             complement(2528..3787)
                     /locus_tag="VIW27_11675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740379.1"
                     /translation="MSASPGTIEAPLLTADGVPLKESLQKSLRQARLRAVLLVAPPLL
                     FLVFVFVIPIGNMLTRSVDDTLINNVLPRTFEAFEAWDKQSDPPEALFDAMHKDLTEA
                     EKIDVGKASVRMNYAEPGWRSLVKRTARRLKRMEPPYKEAFIEVDERWADKTHWLSLG
                     VMKDRATFGYYLNAVDLDFDFDKNIIRQDENRRVYNLLWWRTILVSILVTVACLILAY
                     PVSYLLATLPMRISNLLMICVLMPFWTSLLVRIVAWMIMLQQNGVVNDTLVWSGLLDD
                     DNRLAMMYNFNGTVIVMTQILLPFMILPIFSVMKTIPPSYMKAAQNLGATPTLAFIRV
                     YMPQTIPGVGAGCILVFIVAIGYYITPELVGGKDGRLIGNMVAYHMQRSLNWGLAAAM
                     GTILLVLILILYWIYDRIVGIDNMKMG"
     gene            complement(3851..4930)
                     /locus_tag="VIW27_11680"
     CDS             complement(3851..4930)
                     /locus_tag="VIW27_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5740380.1"
                     /translation="MKLTLKHTLLASVVAVTTAAQAEVNIVSWGGAYTASQQKAYQET
                     YKDPGSINFINYNGGLGEVRTQVESGNVTWDIVDVLPHEGRVGCDEGLFEELDRSLFK
                     PAPDGTSMDDDIMIEVPNDCLVPQIFWSYMPFYQEGTFKGEQPSTIADFFNVKKFPGK
                     RGIHTWPNALIEMALAADGVAIKDIYKVMSTDEGIDRAFKMLDKIKDHAVFWSAGSKP
                     LELVQSGEVSMALAYNGRVGAAILSEGAKFVPVWDGQVLEEEWLVLMKGAPNREAAID
                     FLVHASTTESQAEQARYINYGPMRASAFEVMKAGEPWFHNGKTIMEHMPNRPEVMART
                     IVANPEWWADYGDSIGERYTAWMGQ"
     gene            complement(4996..6093)
                     /locus_tag="VIW27_11685"
     CDS             complement(4996..6093)
                     /locus_tag="VIW27_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740381.1"
                     /translation="MSNDDHLGRFVDIQKSYDGETLVVKNLNLDVARGEFLTMLGPSG
                     SGKTTCLMMLAGFEPATHGEIYLKENPINRVPPHKRGIGMVFQNYALFPHMTVAENLA
                     FPLEVRHMDKSEREERVKRALDMVQLGRFGDRRPAQLSGGQQQRVAVARSLVFDPDLV
                     LMDEPLGALDKNLREQMQYEIKHIHENLGLTVVYVTHDQSEALTMSNRIAVFDDGIIQ
                     QCAPPEVLYEQPDNAFVANFIGENNRLLGEVTEDLGDTVKVAIDADQGTVVAKKVNVE
                     GVGSRSTLSLRPERVTVNPEAGSLPNVFSGVAEELIYLGDHIRTRFTVCGHDDFIVKI
                     PNSAKHAHLKEGDTVNIGWNAEDCRALDPSD"
     gene            complement(6338..6850)
                     /locus_tag="VIW27_11690"
     CDS             complement(6338..6850)
                     /locus_tag="VIW27_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740382.1"
                     /translation="MRFLLAIVLLPGLAQAANIRQPTAITCISDQSSLQGRHKDYHAR
                     KNSEKAKSWFSEWMMAENFGNMRSKIHFDWKPDTPKGPAIEIDTSDKAHNLIQVRSQT
                     KSSLIVVSSASNPLSTESWTFVFNFEVETMIATRVQSNIAGVKGEVITYDCQFQSLEL
                     GALGALERWA"
     gene            6951..7454
                     /locus_tag="VIW27_11695"
     CDS             6951..7454
                     /locus_tag="VIW27_11695"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEY5740383.1"
                     /translation="MRSSNCGIGLLLLVWALVSPLTVAADARADAAQLPRPSTHLQPG
                     EVVRIVIDALAHNDVPYADAGIETTFHFASPANRVVTGPLDRFTRMVKSPTYGIMVNH
                     IDSEFSEVVFSGNRAYQMVRLTAANGLSTVFAFRLGKQLDGEFKDMWMTEAVWPVAEG
                     EASGQAL"
     gene            7533..9119
                     /locus_tag="VIW27_11700"
     CDS             7533..9119
                     /locus_tag="VIW27_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5740384.1"
                     /translation="MNQQFDKPADGEVENASAMLEILTGPGRGTACWLVGGTLDLLLE
                     EDNRIRVIDTGGGADRSDAVARLHRSAGSYEIQALGDNPLWINGERVDSRQLGPRDLI
                     EFGDLGPLTRYRLQWQGERLRRSLGDMLGDAIDYARASRRPKLTRTIVALRDFSRDFV
                     LETTILFRVSVVIALALLAYFSYQQYRSDLRLQQQASSSARQLETFARSLTRASEEAL
                     RPADLNRLRQELRLSLSATAERLELLELRSAASQRIIAAATRATVFLQGAYGYRDIAS
                     GRMLRYQIDDDGNLLFSLRGQPLLTLEGDGEVARRVFTGTAFLVSADGVLLTNRHVAL
                     PWEDDGVEDAPENPAVEPVILRFLGYLPEIEQAFAVEMIEASDETDLAVLEFSQVADE
                     LPFLGLGKGTPQPGAEVIVMGYPTGLRAMLAQTGDRFLAELQEDPSLDFWSIAERLSA
                     AGYIRPLATRGIVGQRSETTVVYDAETTYGGSGGPVLDVDGDVIAINAAIIPEYGGSN
                     FGVPVAYARRLLSAAGREVE"
     gene            9200..9619
                     /locus_tag="VIW27_11705"
     CDS             9200..9619
                     /locus_tag="VIW27_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740385.1"
                     /translation="MKLPALMLFSLLIAACATGYSPTYYMNRVEVVNLTGATIADVSW
                     RVLGSDKSVACAEVAKFSICGEFFPRRRYPQSGIEVKWTHPDGERKTAILNPRVAAYF
                     YRAFPLEIYLEIDAEGSITAYYRQNTPDDGPRIIIDD"
     gene            complement(9694..>10263)
                     /locus_tag="VIW27_11710"
     CDS             complement(9694..>10263)
                     /locus_tag="VIW27_11710"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEY5740386.1"
                     /translation="DLVPAEAEFDDPFTRMSEDQLFELTLVAQIRDRIDAGEEIDADT
                     LETYTEYVASLQQQEVDIDGLLAMREEVTRQRIAKTFLANPQLDGQRVRIPGYLLPLE
                     FDGDKVSEFLLVPWVGACIHTPPPPPNQIVHVTASDGYQSDGGLYTPVWVDGLMKAER
                     NESDLSLVDGTSQIPSLYTIDASSVTLYE"
BASE COUNT         1949 a         3072 c         3239 g         2003 t
ORIGIN      
        1 ggtgatcgac aatgtcgccg gcgacggctt cccggaactg ctcaaactga tgcgccgcgg
       61 cgggcgttac gcatcgtcgg gcgcgatcgc cgggccgctg gtcaatctcg atatgcgcga
      121 tttctacctg tccgacctca acctgatcgg ctgtaccgcc tgggatgaac cggtatttcc
      181 gaacctggtc ggctatatcg agcgcggcga gattcagccg ctgctggcga agacctacgc
      241 gctcgatgag atcgccgtcg cgcaacagga attcctcaag aaaaaccaca tcggcaactt
      301 cgtcctcatc cccccacccc ctaaattggg gtcagagtca ttttttatac aaatttgaat
      361 gactctgacg gcgggcgttc gtttggtttg gtgcggagag aaatgactct gaccccattt
      421 ttcggacccc atttttcgcg ggcagaaatt gactctgacc ccaatttagg ggttagaggt
      481 ggtcggcgat gtagatgaac aggggcaggg tgaagaaggc ggcgattacc tggaaggtta
      541 cgatgctcga catcagcgcc gcgtcgccgc ccatcttgcg cgccatcacg taggccgacg
      601 acgcggtcgg taccgcgccg gcgatgatcg cgacggtgcg cggtaaaccc tcgaggccca
      661 ccagccatga catcgcgatg acgatcgccg gcatgccgat caggcgcgtc agcacaccga
      721 tcgccagcag catcttgtgc tgcgcggcat ggccgaaacg catgccggcg ccgaccgcga
      781 gcaacgccag gccgaggccg ccgtcgccga tgatatcgat ggtcgagaag agtatgtctg
      841 gaatccccag ccagttgaag gcgaggccgg cgccgcaggc gaggatcagc gggttggtga
      901 ggatcttgcg gcccaccgcg gcgaactcgg gccgagtatc gctgcgcccg tagatcgata
      961 aaacgatcac cgtgatcgta ttcagcaacg gtaccatgat cgacagcgcc agtgcgccga
     1021 gggtaacgcc gtcgtcgcca tacagcgggc cgatgatcgc gatggcgacg aaaccgtgaa
     1081 agcgcgtggc cccctggaag atgctggtga agctcggccc gctctcggga tgacgccgcc
     1141 agaccagcgc ctggatcagg atcaacagcg ccgacatgcc gaggatggcg acgatcagca
     1201 cggtcatgaa atcgaggatc ggcagatcgc cgaggtcggc gcgcgacagc gtacgaataa
     1261 tcaggaacgg gaacagcagg taaaagcaga ggtgctcgat cgccgaccac accgattcgc
     1321 cgacgacgcg gctcacggac aacaggtagc cggtgatgat gatcaggaag atcggcgcga
     1381 tggcgttgag cgtggcgatc aatcagcgtt cgcctgcgag tatcgcctcg tagctgcagg
     1441 gaatcgagcg cggttgcagc gagtcggcag agacactcga ttcgaaggtc ttgatgtcct
     1501 gggtgcccgg atcgtagcgg ccgatgcgga tcacgatccc gtcgtcgcgg cacgcgagca
     1561 ccacggccgc gtacttgatc tgattgccgc tgctgtcggt gtaccgatag tacacccggt
     1621 cgccggtctg catcagtgcg gcgtgatacc ccgcaagcgc tcgctgcgac ggcgcagcat
     1681 ttccagcgtg gtcagcagga tgatcgagaa cagtaccagg atggtcgccg cggacaggat
     1741 cgtcgggctg atttcctggc gaatgcccga ccacatctgg cgcggcaggg tatgctgatc
     1801 gggaccgccg atgaacagga tgatgacgat ctcgtcgaac gaagtgatga acgcgaacag
     1861 tgcgcccgag atcataccgg gggcgatcag cggcatctgg atcttgaaga agttatacgt
     1921 cggggtaccg ccgagattgg ccgccgcacg catcaggcca cggtcgaaac cgaccagcgt
     1981 cgaggtcacg gtgatgacca cgaacggcag gccgagaatg gtatgcgcga ggatgatgcc
     2041 ggcgtcggta ttcgacaggt tgacgtcggc aaagaaaaac gccatgccgg cggcggtgat
     2101 gatcagcggc acgatcagcg gcgaaatcat cagcgccatg atggcgcgct tgaacggcat
     2161 gtgctcgctg gaaaggccca gcgccgctag cgtgccaaga aatgtcgcca gcagcgtcga
     2221 gatcacggcg tagtagaggc tgcgcccggc accggtcatc cacttgtcgt ggcacatggt
     2281 cgacgcggcg tcgtcgacgt cggtacagat gccgaccagg ctcttgtacc agcgtaccga
     2341 gaacgcgtcc ggatcgaggg cgagcatctc gggagtgaaa ttcagaaacg gcgacgacgt
     2401 gaacgacagc gggattaccg cgaccagcgg tgcgaccagg aagaacagca caaaggtgca
     2461 aaacgccatg taaaagtaat gccagatgcg ttgtcccggg gttgcgtata tcggtagcgc
     2521 catgtcttca acccatcttc atgttatcga tgccgacgat cctgtcgtag atccagtaaa
     2581 ggatcaggat caggaccagc agtatggtgc ccattgccgc ggcgaggccc cagttcagcg
     2641 agcgctgcat gtggtaggcc accatgttgc cgatcaatcg gccgtccttg ccgccgacca
     2701 gttccggcgt gatgtagtag ccgatggcga cgatgaatac caggatacag ccggcgccga
     2761 cgccaggaat cgtttgcggc atgtagacgc gaatgaatgc cagggtcggg gtcgcaccga
     2821 gattctgcgc cgccttcatg taactcggcg gaatggtttt catgacgctg aatatcggca
     2881 ggatcatgaa cggcagcagg atctgcgtca tgacgatgac cgtgccgttg aagttgtaca
     2941 tcatcgccag ccggttgtcg tcgtcgagca atcccgacca gaccagcgtg tcgttgacca
     3001 ccccgttttg ctgcagcatg atcatccagg ccacgatgcg taccagcagc gaggtccaga
     3061 acggcattaa tacgcagatc atcaacaggt tcgaaatgcg catcggcagt gtcgccagca
     3121 ggtaggaaac cgggtaggcc agaatcaggc aggcgacggt aaccaggata ctgaccagga
     3181 tggtacgcca ccacaacagg ttgtagacgc ggcgattttc gtcctggcga ataatgtttt
     3241 tgtcgaaatc gaagtcgaga tcgaccgcat tcaggtaata gccgaaggtc gcccggtcct
     3301 tcatgacgcc gagcgacagc cagtgtgtct tgtcggccca gcgctcgtcg acctcgatga
     3361 aagcttcctt gtagggtggc tccatgcgct tgaggcggcg cgcggtccgc ttgaccaggc
     3421 tgcgccagcc gggctcggcg taattcatgc gcacgctggc cttaccgacg tcgattttct
     3481 cggcttcggt tagatccttg tgcatcgcat cgaacagcgc ttcgggcggg tcgctctgct
     3541 tgtcccaggc ctcgaaggcc tcgaaggtgc gcggcagaac gttgttaatc agcgtgtcgt
     3601 cgacgctgcg cgtcagcatg ttgccgatcg gaatgacgaa tacaaaaacc aggaatagca
     3661 gtggcggcgc taccagcagg accgcacgca gtctcgcctg gcgcagcgat ttttgcaggc
     3721 tctctttgag cggaacgccg tcggcggtta gcagcggtgc ttcgatagtt ccgggggatg
     3781 cgctcataat tttttcattc gccggttgaa acggggacgg gcccgcggcc cgtccccatt
     3841 gttgattgcg ttactggccc atccaggcag tgtaacgctc gccgatcgag tcgccgtagt
     3901 cggcccacca ctcgggattg gcgacgatag tacgtgccat gacctcgggg cggttcggca
     3961 tgtgctccat aatggttttg ccattgtgga accagggttc gccggccttc atgacttcga
     4021 acgcggacgc gcgcatcggg ccatagttga tgtacctggc ctgctcagcc tgcgactcgg
     4081 tggtcgaggc gtgaaccagg aagtcgatcg ctgcttcgcg gttcggcgcg cctttcatca
     4141 gcaccagcca ttcttcctcg aggacctggc cgtcccagac cggaacgaac ttggctccct
     4201 cggagaggat cgcggcgcca acgcggccgt tgtaggccag cgccatcgag acttcaccgg
     4261 actgcaccag ctcgagcggc ttggagcctg cggaccagaa cacggcatga tccttgatct
     4321 tgtcgagcat cttgaatgcg cggtcgatac cttcgtcggt gctcatgacc ttgtagatat
     4381 ccttgatcgc aacgccgtcg gcggccagcg ccatctcgat cagcgcgttc ggccaggtgt
     4441 ggatgccgcg cttgccgggg aatttcttga cgttgaagaa atcggcgatc gtgctcggct
     4501 gctcaccctt gaaggtgcct tcctggtaga agggcatgta agaccagaag atctgcggta
     4561 ccagacagtc gttgggcacc tcgatcatga tgtcgtcgtc catcgacgtg ccgtcgggtg
     4621 caggcttgaa taggctgcgg tcgagttctt cgaacaggcc ctcgtcgcag ccgacgcggc
     4681 cttcgtgcgg cagcacgtcg acgatatccc aggtcacgtt gccggactcg acctgcgtgc
     4741 gcacctcgcc gaggccgccg ttgtaattga taaagttgat gctaccggga tccttgtagg
     4801 tttcctgata ggctttttgc tgtgaggccg tgtaggcacc gccccatgaa acaatgttca
     4861 cttcagcctg agccgcggtg gtaaccgcaa ccactgaagc cagcagggtg tgtttcaagg
     4921 ttaatttcat cagagttctc tcctcttcgg ttttactgct actgcttcta tctcgggtta
     4981 aaaaaaactg cgcggttaat cggacgggtc caaggcccgg cagtcttccg cattccagcc
     5041 gatattgacc gtgtcacctt ctttgaggtg agcatgcttg gctgaattgg gaatcttgac
     5101 gatgaaatcg tcgtgtccgc agaccgtgaa acgggtgcgg atatggtcgc cgagataaat
     5161 cagttcctcg gcaacacctg aaaatacgtt cggcagcgag ccggcttccg ggttcacggt
     5221 gacgcgttcc gggcgcagcg acagggtgct gcggctgccg acgccctcga cattgacttt
     5281 cttggcgacc accgtgccct ggtcggcgtc gatggcgacc ttgacggtgt cgccgagatc
     5341 ctcggtgacc tcgccgagca ggcggttgtt ctcgccgatg aaattggcga cgaaggcgtt
     5401 atcgggctgc tcgtagagca cctccggcgg cgcacactgc tgaatgatgc catcatcgaa
     5461 caccgcgatg cggttagaca tggtcagcgc ctcgctctgg tcgtgggtca cgtagactac
     5521 ggtcaggccg aggttttcgt ggatatgctt gatctcgtac tgcatctgct cgcgcaggtt
     5581 cttgtcgagc gcacccagcg gctcgtccat caacaccagg tcggggtcga agaccagcga
     5641 gcgcgccacc gcgacccgct gttgctggcc gccggagagt tgcgccggcc ggcgatcgcc
     5701 gaacctgccc agctgcacca tgtcgagcgc gcgcttgacg cgctcctcgc gctcgctctt
     5761 gtccatgtgg cgcacctcga gcggaaatgc caggttctcg gccactgtca tgtgcgggaa
     5821 cagggcgtag ttctggaata ccatgccgat gccgcgcttg tgcggcggca cccggttgat
     5881 cgggttctcc ttgaggtaga tctcgccgtg cgtcgccggt tcgaagcccg ccagcatcat
     5941 caggcaagtg gtcttgccgg agcccgacgg cccgagcatg gtcaggaact cgccgcgggc
     6001 gacatccaga ttgaggtttt ttactaccag tgtttcgccg tcgtaactct tttgtatatc
     6061 gacgaagcga cctagatggt catcattcga catggcgtgt cagtctcggt cgcaaattgt
     6121 gacgctattg ttatattgtc cgggtggacg agtggtttcg atgcccggat cctttggtaa
     6181 accgcaacct tagccgtcct agccggcaaa atcaaccgtt gcccgataat cggttttttg
     6241 gtcatagatc gcagcgtttt ctcaccaatt tggattattt cgaggaaata ctgctgcgga
     6301 tcgggttaac gccggctgaa tgccgcgggc gcggcattca cgcccatctt tcgagcgcgc
     6361 cgagcgcgcc gagctcgagg ctctggaact ggcagtcgta ggtgatgacc tcgcccttga
     6421 cgccggcgat gttgctctgc acgcgggtgg cgatcatggt ctcgacttcg aagttgaaca
     6481 cgaaggtcca gctctcggtg gacagtggat tcgatgccga ggagaccacg atcaggctgc
     6541 ttttggtctg cgagcggacc tggatcagat tgtgagcctt gtcgctggta tcgatctcga
     6601 tagccgggcc cttgggcgtg tcgggcttcc agtcgaagtg aatcttggat ctcatgttgc
     6661 cgaaattttc agccatcatc cactcggaga accaggactt ggctttttcc gaattcttgc
     6721 gcgcgtggta atccttgtgg cgcccctgca ggctcgactg gtcgctgatg caggtgattg
     6781 cggtcggctg tcgaatattc gctgcctgag cgagccccgg caacagcacg atggcgagta
     6841 gaaatcgcat cggtccagta taccggtttg cttcccgatt gacagtcgcc cccggcgagg
     6901 cctgacaagc ggccaccaaa agcgtattct cgtacccgga gggcaggatt atgcgaagct
     6961 caaactgcgg aatcggctta ctgctgctgg tatgggcact ggtctcgccg ctgacggtgg
     7021 ccgccgacgc ccgagcggac gccgcgcaat tgccgcgacc gtccacccat ctgcagcccg
     7081 gcgaggtggt gcgcatcgtc atcgatgcgc tggcgcacaa cgacgtcccc tatgccgatg
     7141 ccggtatcga gaccaccttt cacttcgctt cgccggccaa ccgggtcgtc accgggccgc
     7201 tcgaccgctt tacgcgcatg gtcaagagcc cgacttacgg aatcatggtc aaccacatcg
     7261 acagtgaatt cagcgaggtc gtgttctccg gcaacagggc ttaccagatg gtacgcctga
     7321 ccgccgccaa cggcttgtcg accgtgttcg cgttccggct cggcaagcaa ctcgatggcg
     7381 agttcaagga tatgtggatg accgaggcgg tgtggccggt ggccgaaggc gaggcctcgg
     7441 ggcaggcctt gtagcgccgt cggccggcgg cggcgatcgg ctgcgaattc gccgcgccgg
     7501 caaggcatcg gaaacagggc gtattcggtt tcatgaacca gcagttcgac aaacccgcag
     7561 acggcgaagt tgaaaatgcc tcggcgatgc tggagatcct caccggaccg gggcgcggta
     7621 cggcgtgctg gctggttggc ggtacgctcg acctgctgct cgaggaggac aacaggattc
     7681 gggtcatcga taccggcggc ggtgcggacc gctccgatgc cgtcgcgcga ctgcaccgct
     7741 cggcgggcag ctacgaaatc caggctctcg gcgacaaccc tctatggatc aacggcgagc
     7801 gcgtcgacag ccggcagctc gggccgcgcg acctgatcga gttcggcgac ctggggccgc
     7861 tgacgcgcta ccggttgcag tggcaggggg agcggctgcg gcgatccctt ggcgacatgc
     7921 tcggcgatgc gatcgattac gcgcgtgcca gccgccgccc gaaactgact cgaaccatcg
     7981 tcgcgctgcg cgatttcagc cgtgatttcg tgctcgagac gaccatcctg ttccgtgtca
     8041 gcgtggtcat cgcgctggcc ttgctggcgt atttctctta ccagcaatac cgctccgatc
     8101 ttcgcctgca gcaacaggcc agcagcagcg cgcgccagct ggagaccttc gcgcgcagcc
     8161 tgacgcgcgc cagcgaggaa gcattgcggc ccgccgacct gaaccgcctg cgccaggagt
     8221 tgcggctgtc gctgtccgcc accgcggagc ggctcgagct gctcgagctg cgctcggccg
     8281 ccagccagcg aatcatcgcc gcggcgaccc gcgccacggt attcctgcag ggcgcctacg
     8341 gctaccgcga cattgccagc gggcgcatgc tgcgctacca gatcgacgac gacggcaacc
     8401 tgttgttttc gctgcggggg cagccgctgt tgacgctgga gggcgacggc gaggttgcgc
     8461 gccgcgtatt caccggtacc gcgttcctgg tcagcgcgga cggcgtgctg ctgaccaatc
     8521 ggcatgtggc actgccgtgg gaagacgacg gtgtcgagga tgcgccggaa aacccagcgg
     8581 tcgaaccggt aatcctgcgt tttctcggct acctgcctga gatcgagcag gcgttcgcgg
     8641 tcgaaatgat cgaggccagc gacgagaccg acctggcggt gctcgaattc agccaggtcg
     8701 ccgacgagtt gccgttcctc ggtctcggca agggaacgcc acaaccggga gccgaagtga
     8761 tcgtcatggg ttacccgacc ggattgcgcg ccatgctggc acagaccggt gaccgctttc
     8821 tcgccgagct gcaggaagac ccatcgctcg atttctggag tatcgcggag cgcctgtcgg
     8881 ctgccggcta cattcgcccg ctggccaccc gcggcatcgt cgggcagcgc tccgagacga
     8941 ccgtggttta cgatgccgag accacctacg gcggcagcgg cggcccggtg ctcgacgtcg
     9001 acggcgacgt gatcgcgatc aacgcggcga tcattcccga gtacggcggc tccaatttcg
     9061 gcgttccggt agcctatgca cgccgcctgc tgagtgccgc cggccgcgag gtggagtgag
     9121 gcgtaagcgg accgctcccg atcccggaaa actgcatcgg catcgaggtt atcgtcaacg
     9181 tgaaaggagc tacgacaaca tgaaattgcc agccctgatg ctattcagcc tgttgatcgc
     9241 ggcttgcgca accggctaca gccccaccta ctatatgaac cgggtcgagg tcgtcaatct
     9301 caccggggcg acgatcgcgg acgtcagctg gcgggtgctc gggtccgaca aatccgtcgc
     9361 ttgcgccgag gtcgccaaat tttcgatttg cggcgaattc tttccccggc ggcgctaccc
     9421 gcaatccggg atcgaggtca agtggaccca tcccgacggc gagcgcaaga ccgcgattct
     9481 gaatccgcgg gtagcggctt acttttaccg ggcattcccg ctcgaaattt acctggaaat
     9541 cgatgccgag ggttcgatta cagcctatta ccggcagaac acgccggacg acggcccgcg
     9601 gattatcatc gatgactgat tcgtcagccg catgacgagc cggattccag gactccgcct
     9661 gagcgtaccg cacaccgccc gggcgcacgc agcttactcg tagagcgtca ccgacgaggc
     9721 atcgatcgta tacaggctcg ggatttgcga agtgccgtcg accaggctca ggtcggattc
     9781 gttgcgctcg gccttcatca gcccgtcgac ccataccggg gtgtataacc cgccgtcgct
     9841 ctggtagcca tcgctggcgg tgacgtgaac gatctggttg ggcggtggcg gcggcgtatg
     9901 gatacaggca ccgacccagg gcaccagcag aaattcactg accttgtcgc cgtcgaattc
     9961 gagcggcagc aggtaaccgg gaatccgcac tcgctgcccg tcgagctgcg gattggcgag
    10021 aaaagtcttg gcgatgcgct gccgggtaac ctcctcgcgc atcgccagca ggccatcgat
    10081 gtcgacctcc tgctgctgca gcgaggcgac atattcggtg taggtttcga gggtatcggc
    10141 gtcgatttcc tcaccggcgt cgatccggtc cctgatctgc gctaccagcg tcaattcgaa
    10201 tagctggtcc tcgctcatcc gcgtgaacgg gtcgtcgaac tcggcctcgg cgggcaccag
    10261 gtc
//
LOCUS       DATCZV010000517         3870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_518349, whole genome shotgun sequence.
ACCESSION   DATCZV010000517 DATCZV010000000
VERSION     DATCZV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518349"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..78)
                     /locus_tag="VIW27_11715"
     tRNA            complement(3..78)
                     /locus_tag="VIW27_11715"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Glu,seq:ttc)
     gene            complement(98..173)
                     /locus_tag="VIW27_11720"
     tRNA            complement(98..173)
                     /locus_tag="VIW27_11720"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138..140),aa:Ala,seq:ggc)
     gene            612..1469
                     /locus_tag="VIW27_11725"
     CDS             612..1469
                     /locus_tag="VIW27_11725"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY5740387.1"
                     /translation="MLFRVLGDKWLRLVVLLLVLAALAHQVSLVVWQLVPVEPQSGLM
                     PAPGIQQASTAMGNESGESYQQQAAAIGRAYLFGRAEVKAVPQPEVEDAPETQLNYKL
                     RGTYYSPADRLSVAIIEVKPNQSRHYIVDEELDDNIVLTRIEEDYVLLNRYGKIERLN
                     LEKRGAGTGQAISATDSRAQAGQTALLRSYKRRYTSNPMALATRFQAIPVQQDGKNIG
                     FKLKALRGEKLLERLDFEPDDVFTAVNGVSLQNPFEALDALKSLTTADSVSVTFMRNG
                     TEQTLDFQI"
     gene            1507..3411
                     /gene="gspD"
                     /locus_tag="VIW27_11730"
     CDS             1507..3411
                     /gene="gspD"
                     /locus_tag="VIW27_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY5740388.1"
                     /translation="MALLLAALMRPAAAEDVTLNFSDADLVSVINSVSQITGKNFIID
                     PRVKGKINVVSSKPLNEDEVYNVFLSILQVHGFATIPGENAIKIIPDATAKQSETPFD
                     AASRNPGDQLITRVLQIENINAAQLVPILRPLVAQQGHLAAYPATNVLIISDRAANIL
                     RIDRIIQQMDKKGDSGIEIIKLQNAFAAEVVRLLTGLNVTTPEQKAQGGGVSITADER
                     TNSILLSGERAERLKYRAIIADLDSPVASSGNTHVVYLRYAEATDVAALLNNVGQEVI
                     KAEGKNVGTVGGAPAGGETVSVQADEASNALVITAPASVYPSLRAVIQQLDIRRAQVH
                     IEAIIAEVSLDTSNELGVQWLASDQDSGAFSVQFSSAGATIPSIANGGGLPDGGVMGL
                     GTIDDDGLSIVALVSALAGDSETNLLATPSLVTIDNQEAEIIVGQNVPFVTGESTTAG
                     ADQQNPFRTIERQDIGVSLKVKPQINEGDAITMAIEQEVSNISGTATSAVDIVTNKRS
                     IKTTVQLEDGALLVLGGLIDETVIVSEQKVPGLGDIPVLGALFRYKKSSKVKQNLLVF
                     IRATIIKDPDKAHLLSYRKYNFMRELQINAMGGELDQMAPALLPYEGEIPELLDQRRL
                     PNSLAVPDDN"
     gene            3536..>3870
                     /locus_tag="VIW27_11735"
     CDS             3536..>3870
                     /locus_tag="VIW27_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY5740389.1"
                     /translation="MAKGFAHLGLLPALLFAHHGANAATLGSLLISEIMANPAVVSDA
                     RGEWFELYNPTGQQFNLRDITIGDDGSDRHRIETDLLISPGHFLTLARNGDPTINGGF
                     AADYVYRNFT"
BASE COUNT          850 a         1183 c         1140 g          697 t
ORIGIN      
        1 agtggcgtcc ccaaggggat tcgaacccct gttgccgccg tgaaagggcg gagtcctagg
       61 cctctagacg atggggacac tggataaaac gtaaatctgg tggagccagg cgggatcgaa
      121 ccgccgacct caacactgcc agtgttgcgc tctcccagct gagctatggc cccaaatgag
      181 agagcgcgta ctttaatcaa gcgcccgggg actgtcaaga atacagcgcg tgaaaataac
      241 taaatatcaa gcatttgtcg cttttcgttc gcgggtggtt taatgggttg ccgatcggct
      301 ggcgcgggct tgccgtgaag tcccttacga atccgttgcg agtccgtgat gcgtatcaat
      361 gatgagcatg ccgattccgg ttaccgtcgc tgccgacgat aattccggta attgttcatt
      421 gaaatcgggc aagattcgcg tttaactgat tttcacccaa aagataaatg attcggctac
      481 aattaaatcg tttaaaatca ataaattcga cagtaaactt gaatttaaat atagtttctg
      541 tgagcagggc gaatataata ccgggccgcg gttgcggatt ttgaccaggg ggcagacgtt
      601 tgactccaga aatgcttttc agggtgctcg gcgacaagtg gttgcggctc gtcgtgctgc
      661 tgctggtgct cgcggcactg gcacaccagg tatcgctggt cgtctggcag ctggtgccgg
      721 tggagccgca gtccggcctg atgccagcgc ccggcattca gcaggcatcg accgcgatgg
      781 gcaacgagag cggggagagt tatcagcagc aggccgccgc gatcggccgc gcctacctgt
      841 tcggccgcgc cgaggtcaag gcggttccgc agcccgaggt cgaggacgct ccggaaaccc
      901 agctcaatta caagttgcgc ggcacgtatt actcgcccgc cgatcgcctg tcggtggcca
      961 tcatcgaggt caagcccaac cagagccggc attacatcgt cgacgaggaa ctggacgaca
     1021 acatcgtgtt gacacgcatc gaggaagact acgtgctgct caaccgttac ggcaagatcg
     1081 agcggctcaa cctcgaaaag cgcggcgcag gcacgggcca ggcgatcagc gccacggaca
     1141 gccgcgcgca ggccgggcag acggccctgc tgcgcagcta caagcggcgc tacaccagca
     1201 atccgatggc attggcgacg cgtttccagg ccatcccggt gcagcaggat ggcaagaaca
     1261 tcggcttcaa gctgaaggcg ctgcgtggcg aaaagctgct ggaaaggctc gacttcgaac
     1321 ccgacgacgt atttaccgcg gtcaatggcg tatcgctgca gaacccgttc gaagcgctcg
     1381 acgcgctcaa gtcgctgacc accgccgata gcgtctcggt gacctttatg cgcaacggca
     1441 ccgaacaaac cctcgatttc cagatttaac aggccgcgga caatgaacaa gcactcgata
     1501 ctgattatgg cgctcctcct cgcggcgctg atgcggccgg cagcggccga ggatgtcacg
     1561 ctaaatttca gtgacgcgga cctggtgtcg gtgatcaaca gcgtgtcgca gatcaccggc
     1621 aagaacttca tcatcgaccc gcgggtcaag ggcaagatca acgtcgtctc ctccaagccg
     1681 ctcaacgagg acgaggttta caacgtcttc ctgtcgatac tgcaggttca cgggttcgcc
     1741 accatccccg gcgaaaacgc gatcaagatc atccccgatg ctaccgccaa gcagagcgag
     1801 acgcccttcg acgcggccag tcgcaacccg ggcgaccagc tgatcacccg cgtgttgcag
     1861 atcgaaaaca tcaatgccgc acagctggtg ccgatcctgc ggccgctggt cgcgcagcag
     1921 gggcacctgg cggcataccc ggccaccaac gtgctgatca tttccgatcg cgccgcgaat
     1981 attctgcgca tcgaccgcat cattcagcag atggacaaga agggcgattc gggtatcgag
     2041 atcatcaagc tccagaacgc cttcgccgcc gaggtggtgc gcctgctgac cggcctcaac
     2101 gtgaccacac cggagcaaaa ggcacagggc ggcggcgtca gcatcaccgc cgacgagcgc
     2161 accaacagca tcctgctcag cggcgagcgc gccgaacgcc tgaagtaccg cgcgattatc
     2221 gccgacctgg attcgccggt ggcctcgagc ggcaacaccc acgtggtcta cctgcgttac
     2281 gccgaggcca ccgatgtcgc ggcactgctc aacaacgtcg gccaggaagt catcaaggcc
     2341 gagggcaaga acgtcggcac cgtcggcggg gcgcccgccg gcggagagac cgtcagcgta
     2401 caggctgacg aagcctccaa tgcactggtg attaccgcgc cggcctcggt atacccgtcg
     2461 ttgcgcgcag tcatccagca gctcgacata cggcgcgcgc aggtgcatat cgaggcaatc
     2521 atcgccgagg tctcactgga tacctccaac gagctcggcg tgcaatggct tgccagcgac
     2581 caggattcgg gcgctttctc ggtgcaattt tcgagtgccg gcgcgaccat tccgagcatc
     2641 gccaacggcg gcggactacc ggacggcggc gtcatgggtc tcggcaccat cgacgacgac
     2701 ggtctcagca tcgtcgcgct ggtcagcgcg ctggccggcg actcggaaac caacctgctg
     2761 gcgacaccgt cactggtgac catcgacaac caggaagcgg aaatcatcgt cggccagaac
     2821 gtacccttcg taaccggaga atccactacg gcaggtgccg atcagcaaaa tccgtttcgg
     2881 accatcgagc gccaggacat cggcgtcagc ctcaaggtca agccgcagat caacgagggc
     2941 gacgcgatca ccatggcgat cgaacaggaa gtctctaaca tttccggcac cgcgaccagc
     3001 gcggtggata tcgttaccaa taagcgctcg atcaaaacta ccgtgcagct cgaggacggc
     3061 gcgttgctgg tgctcggcgg gctgatcgac gagaccgtca tcgtcagtga acaaaaagtg
     3121 ccgggtctcg gcgacatacc ggtgctcggc gcgctgtttc gttacaagaa atcttcgaag
     3181 gtcaagcaga acctgctggt gttcattcgc gccaccatca tcaaggatcc cgacaaggcg
     3241 cacctgctga gctaccgcaa gtacaacttc atgcgcgaac tgcagatcaa cgcgatgggc
     3301 ggcgagctgg accagatggc gccggcactg ttgccctacg agggcgagat acccgagttg
     3361 ctcgaccaac ggcgcttgcc caatagcctt gccgttcccg acgacaacta gtatcggaat
     3421 tatcctacag gactttacgc cgtttgcgct ggttcggcgt gcgcgctgtt gctaagtttc
     3481 gtcctgcaac cagttgcacg gaagccaccc tataaacaaa tgacaggagg tcatcatggc
     3541 aaagggattt gcccatctcg ggcttttgcc ggcattgctt ttcgcgcatc acggcgctaa
     3601 cgcggcgaca ctcggctcgc tgttgatcag cgaaatcatg gctaacccgg cggtggtgag
     3661 cgatgcccgg ggcgaatggt tcgagctcta caatccgacc gggcagcaat tcaacctgcg
     3721 ggatatcacg atcggcgacg acggcagcga ccgtcaccgt atcgaaaccg acctgctgat
     3781 ttcaccgggg catttcctga ccctggcgcg caacggcgac ccgacgatca acggcggctt
     3841 cgctgccgac tacgtgtacc gcaatttcac
//
LOCUS       DATCZV010000518         6726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_306699, whole genome shotgun sequence.
ACCESSION   DATCZV010000518 DATCZV010000000
VERSION     DATCZV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306699"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VIW27_11740"
     CDS             <1..267
                     /locus_tag="VIW27_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5740390.1"
                     /translation="VGVLSKKDCLEIVYHTAYHQDWGGQVQQYMSRNVETIDADSSIL
                     DAAEKFLSSSYRRFPVLRDGRLVGQISRCDLLRAIDEVYLRDRR"
     gene            complement(359..559)
                     /locus_tag="VIW27_11745"
     CDS             complement(359..559)
                     /locus_tag="VIW27_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740391.1"
                     /translation="MDVCAEQLRFQAFGTPIEATYSHYSDCRGYTGAKVAVWATFDTW
                     KVRFSKAGKKVRVATGNHPRIL"
     gene            951..2273
                     /locus_tag="VIW27_11750"
     CDS             951..2273
                     /locus_tag="VIW27_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740392.1"
                     /translation="MANSESLARNSVTVDYAILHRPATLVAIALLLFLLQLILAVSIL
                     DIVAPYNLRGMWITIPDFPDRLNDTGSMLERVFSRLNGWDGHWYHHIASNGYQCSAIP
                     ESNNPYQCNVAFFPLLSMLGAGLGSLGIDLLYALPLVSQLAWFATILAILWFARSITT
                     LHPLHLLVLLLLVAYPGALYGFVAYSESLLTLLVVLITILAHHYLANPRWPLLAGLVL
                     CCFMLGLVKGTGLVAAAIPVLMALLHPRFDGKWFAREQLLVYAASSASLAGLLAFFLY
                     SEIRFGDWALYFRYVGSAWGSDGTLRLNPLHLVTNFSWHDILAVRLSNVIAVTMPLAI
                     GVVAAVSLRFAVQTRYLSLAVLGTVLMLFYVYTVPGNNGQFTNFNLMRHMLPLVALLV
                     LLAMTLPLSKLKQQTAGIIALVLLGLVYLQFHFQMEMFHAFKFGYWVS"
     gene            2646..3053
                     /locus_tag="VIW27_11755"
     CDS             2646..3053
                     /locus_tag="VIW27_11755"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5740393.1"
                     /translation="MGRRYHSVDRRTEPGRRGPWARPLAVALSSLLGLAAAGALHAQV
                     PVDERHGDCLFCHMSDEQSDGSLILPEPEVCWLCHAPGNDHAILIPATTADPSLPLNN
                     GLMTCITCHDPHSTQALQLRLPTTGLCAACHDI"
     gene            complement(3181..4371)
                     /locus_tag="VIW27_11760"
     CDS             complement(3181..4371)
                     /locus_tag="VIW27_11760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5740394.1"
                     /translation="MSKAGNENIDFSGVEQVAERYVAERKSAGMLTLVSRQGEVVHRH
                     CAGHADVEHAIALREDTIFRIYSMTKPLTSVALLQLIERGLCALDDAASRWIPALGEL
                     EVHDEGPLQSDITIRQLLTHTAGFSYGFEPDKYAVDKLYAEIWQQGIQHRTLEDLMGS
                     LLQLPLLAQPGTRWHYSIATDVCGYLVERLTDMPFGDYLRSRIIEPLGMVDTDFEVSA
                     ERAARFATLYGPTEQNSLTPLEPPDRSPFVPSLSGVPVQLHSGGGGLVSTAPDYLRFA
                     QMILGGGELDGQRFLAAETVALMTQNHVDERLLPLTFNGIAPGEYRGYGFGLGYCVTI
                     DTAATAAAGSRGDFGWGGMADTYCWIDPAEQLIGILMQQHLPSLVHPGRKDFRDAVYA
                     AISG"
     gene            complement(4368..5657)
                     /locus_tag="VIW27_11765"
     CDS             complement(4368..5657)
                     /locus_tag="VIW27_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5740395.1"
                     /translation="MNDDQLIERRRNTLGPTYQNFFDRPLHLVRGAGTALWDADGRQY
                     LDCYNNVVSVGHCHPRVVDALCRQAATLNTHTRYLHAGIVELGEMLVERLPGRLDTCV
                     FTCTGSEANDLAAQIARQVTGNYGLVVTEASYHGVSELTRRMSTDSYPAAERPDWLAV
                     IEPPNLYRGPYRRDDPQAGEKYLELARLELDRLEERGYRPAAVMIDLVWDSNGPLTAP
                     PGYVAGLGAEIRKRGGLVIADEVQSGYCRTGEHWWASDNYRIEPDILTCGKPMGAGHP
                     LALMATRHEIAEAYANARHYFNTFGGNPVSAAVGKAVIEVIEDEGLLDNARTSGAYLQ
                     AGLRELASRHSLIGDVHGCGLFWGLDLVSDAATREPLPRDALRHLGSLIAERGVITGT
                     SGRYGQVLKLRPPLPFSTADADIALAAIDSALEVFQP"
     gene            complement(5678..5941)
                     /locus_tag="VIW27_11770"
     CDS             complement(5678..5941)
                     /locus_tag="VIW27_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740396.1"
                     /translation="MPHKHGWGHTLISQVRQLATDAEVGALVMFHHDPERSDSELDEI
                     QLENERYLKSRRAPTHSLCAAEGMRIELSKPLLGGETRIRIDP"
     gene            complement(5993..6565)
                     /locus_tag="VIW27_11775"
     CDS             complement(5993..6565)
                     /locus_tag="VIW27_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5740397.1"
                     /translation="MNITFYGVRGSTAVPGPQTAKYGGNTSCVHIELETGNDLILDAG
                     TGIRLLGQKLARKSTPANILLSHGHWDHIQGYPFFEPIYQPDRDINVYISVESGRKLL
                     STLFEQMDGTHFPVRAEDLPSNNIAKFKGVESELYEREGINVVKRGLNHPGGGVAYRI
                     SENGISVAYVTDNELEPPYPPTTSYDQWVN"
BASE COUNT         1154 a         2091 c         2103 g         1378 t
ORIGIN      
        1 gtgggggtat tgtcgaagaa ggattgcctg gagatcgtct accataccgc ttaccaccag
       61 gattggggtg ggcaggtgca acagtacatg agccgtaacg tcgaaaccat cgatgccgac
      121 agcagtattc tcgacgccgc ggaaaaattc ctctcctcca gctaccgtcg tttcccggtg
      181 ctgcgcgacg ggcggctggt cggacagatc agccgctgcg acctgctgcg cgcgatcgac
      241 gaggtttatc ttcgcgacag gcgctgagct tcaggcccga gcctgaaccg gtattttcga
      301 taccctcgag tagcggatcg atcgcgattg ccgtcgctat ttcgagctgc ctcggttatc
      361 acaaaatccg cggatgattt ccggtggcga ctcgaacttt tttgcctgct ttcgagaagc
      421 gcactttcca ggtgtcgaag gtcgcccaaa ccgccacctt tgctccggta tatcccctgc
      481 aatcggaata gtggctgtag gttgcttcga tcggtgtacc gaatgcctgg aaccttaatt
      541 gctccgcaca gacatccacc agctgctcgt gctgcttcca tgaagctgtt catcgacgga
      601 gagcaggggc tgccatcgaa tatgcaggcc ggccacgtgg atcaatcggc gtggaccgtc
      661 gagcgacgag ggtgtgactg cagggggcgg cggcccacag ggtgcccgct tttgaaatgc
      721 cgccccggct gcccgcatta tcggcaagca gtgtcgggat gatgccgatc gagaagatgg
      781 catttctcaa tgacatcaac aaaaccacca gagctccgag caatcgcttc gctagcgccg
      841 gcaaaaaggc gcccgtttcg ttcgccaact gctagacttt gactaatcca ggccgcatcc
      901 tgcgatcacg ccgcatgcgg tcacaccacg gacaaatcga caaagttatt atggccaaca
      961 gcgaatctct cgcgcgcaac agcgtcaccg tcgactatgc gatcctgcac cggccggcga
     1021 ctctggtcgc aatcgccttg ctgctgttcc tgctgcagtt gattctggcc gtatccatcc
     1081 tcgatatcgt cgctccttac aatctgcgcg gcatgtggat tacgattccc gactttccag
     1141 atcggttgaa cgacaccggt agcatgctcg agcgggtatt cagccgctta aacggctggg
     1201 atggccactg gtaccatcat attgcaagca acgggtacca gtgcagcgca attccagaaa
     1261 gcaacaatcc ttaccaatgc aacgtcgctt tcttcccgct gttatcgatg ctgggtgcag
     1321 gcctcggctc gctgggtatc gacctgttgt atgcgctgcc cctggtgtcg caactggcct
     1381 ggttcgcgac catcctggcg attctgtggt ttgcgcgcag catcacgacg ctgcaccccc
     1441 tgcacctgct ggtgttgctg ctgctggtcg cttatcccgg ggctctttac ggcttcgtcg
     1501 cctacagcga atcgctgctg accctgctcg tcgtcctgat caccatcctg gctcaccact
     1561 acctggccaa tccgcgctgg ccgctgctgg ccggcctggt cctgtgctgc ttcatgctgg
     1621 gtctggtcaa ggggacgggg cttgtggccg ccgcgatacc cgtgctgatg gcgctgttgc
     1681 atccccgctt cgacggcaag tggttcgccc gcgaacagct gctggtctat gctgcgagtt
     1741 cggccagtct ggcgggtctc ctcgctttct tcctctacag cgaaattcgc ttcggtgatt
     1801 gggcgctgta ttttcgctac gtgggttcgg cctggggctc cgatggaacc cttcggctca
     1861 acccgctgca cctggttacc aatttcagct ggcacgacat tctggcggtg cgattgtcga
     1921 acgtgatcgc cgtaaccatg cccctggcca ttggcgtcgt ggccgcggtg tcgcttcgtt
     1981 ttgccgtgca aacccgctat ctcagcctgg cggtcctcgg caccgtgctg atgctgttct
     2041 atgtctacac cgtaccgggc aacaatggtc agttcaccaa cttcaacctc atgcgccaca
     2101 tgctgccgct ggtggcactc ctggtcctgc tggcaatgac cttgccgctg tcgaagctca
     2161 agcagcagac cgcgggcatc atcgcactgg tattgctcgg gcttgtctac ctgcagtttc
     2221 attttcaaat ggaaatgttt cacgcattca aattcggata ctgggttagc tgacattcga
     2281 accgggcggc aagccaggcc gtcaaacccg gtggctcgag gatagcgtgg gcggtgccat
     2341 cccggtatcg tcgccatcgt tcggcaatcg aagccgctgc cttccggtaa tgtgcggcgg
     2401 catccttgtc ggccggatcg gccgcggcga atagcagtgc gcgctcgacg catatcgtcg
     2461 aatttgcccc aatcgtcggc agcgggcttc gaaaatgctt gctaccggtt accggtttga
     2521 tgcaaatcaa aacaatttct accgatcgtt acacgctatt gactgtgatc gatgaatgca
     2581 gataagccgg tctcggcgac tcccgcctgg aggcacggcg tggccgagag gtaacggaac
     2641 agcgagtggg taggcgttat cacagcgttg acagacgaac cgagccgggt cgccgcgggc
     2701 cctgggcacg gccgctggca gtcgcgctgt cgtcgttact gggcctggca gcggccgggg
     2761 ccctgcacgc ccaggtaccg gtcgacgagc gacacggtga ctgcctcttt tgccacatga
     2821 gcgacgaaca aagcgacggc tcgctgatac tccccgagcc ggaagtgtgc tggctctgcc
     2881 atgcgcccgg taacgatcac gcgatattga tcccggcgac gacggcagat ccgtcgctgc
     2941 ccctgaacaa tggtttgatg acctgtatca cctgccacga tccgcattcg acgcaagcct
     3001 tgcagttgcg cctgccgacc accgggctat gcgccgcctg ccatgacatc tgaagtttgc
     3061 cgtggcgccg agcatcgacc gcccgtgcgg cgccgccaca gcacgcggta ccctgacacc
     3121 cggtcgcggc tgcaaatgca tggcggcgga gccgcgtagc tgtcaacgtc aacgtcgatg
     3181 tcagccgctt atcgccgcgt agaccgcgtc gcgaaaatcc ttgcgcccgg ggtgcaccag
     3241 cgacggcagg tgctgctgca tcaggatgcc gatcagttgc tcggccgggt cgatccagca
     3301 gtaggtatcg gccatgccgc cccagccgaa atcgccgcgc gatcccgctg ccgcggtggc
     3361 ggcggtatcg atggtgacgc agtaaccgag gccgaatccg taaccgcgat attcgcccgg
     3421 ggcgatgccg ttgaacgtca gcggcagcag ccgttcgtcg acgtggtttt gcgtcatcag
     3481 cgcaacggtc tcggcggcga ggaagcgctg cccgtcgagt tcgccgccgc ccagtatcat
     3541 ttgcgcgaag cgcaggtagt cgggcgcggt cgataccagt ccgccaccac ccgagtgcag
     3601 ctgcaccggc acgccggaca gtgacggcac gaacggcgag cggtcggggg gttccagcgg
     3661 cgtcagtgag ttttgctcgg tgggcccgta gagcgtcgca aagcgcgcgg cacgctcggc
     3721 tgaaacctcg aaatccgtat ccaccatacc cagcggctcg atgatgcgcg agcgcaggta
     3781 gtcgccgaac ggcatgtcgg tgagccgctc gaccaggtat ccgcagacat ccgtggcgat
     3841 gctgtaatgc cagcgcgtgc cgggctgcgc cagcagcggc agttgcagca ggctgcccat
     3901 caggtcttcg agggttcggt gttgaatgcc ctgctgccag atctcagcat aaagtttgtc
     3961 gacagcatat ttgtccggtt cgaagccgta gctgaagccg gcggtatggg tcagcaactg
     4021 gcgaatcgtg atatcgcttt gcagcgggcc ttcgtcgtgc acttcgagct cgccgagcgc
     4081 cggaatccag cggctggcgg cgtcgtcgag cgcgcacagc ccgcgctcga tgagttgcag
     4141 cagcgcgacg ctggtcagcg gcttggtcat cgagtagatg cgaaagatcg tgtcctcgcg
     4201 caacgctatc gcgtgttcga catcggcatg gccggcacag tggcggtgca ccacctcgcc
     4261 ttggcggctg accagcgtca gcatgccggc cgatttgcgc tcggcgacgt agcgctcggc
     4321 gacctgttcg acgccgctga aatcgatgtt ctcgttgccg gccttgctca tggctgaaaa
     4381 acctcgaggg cggagtcgat cgcggccagc gcgatatcgg cgtcggccgt ggagaacggc
     4441 agtggcgggc gcagctttaa cacctgtccg tagcgtcccg aggtgccggt gatcacgccg
     4501 cgctcggcga tcaggctgcc gaggtggcgc agcgcgtcgc gaggcagcgg ttcgcgggtc
     4561 gcggcatcgg acaccaggtc gaggccccag aacagcccgc agccgtgcac gtcgccgatc
     4621 agcgagtggc gcgacgcaag ctcgcgcagg cctgcttgca ggtaagcgcc gctagtgcgc
     4681 gcgttgtcga gcaggccttc gtcctcgatg acctcgatca ccgccttgcc gactgctgcc
     4741 gacaccgggt taccgccgaa ggtattgaag taatgacgcg cgttagcgta agcctcggcg
     4801 atctcgtgcc gcgtcgccat caatgccagc gggtgaccgg cgcccatcgg tttgccgcag
     4861 gtgaggatgt ccggctcgat gcgataattg tcgctggccc accagtgctc gccggtgcgg
     4921 cagtaaccgg attgcacctc gtcggcgatc accagcccgc cgcgtttgcg aatttcggcg
     4981 ccgaggccgg cgacgtagcc gggcggcgct gtcagcggcc cgttggaatc ccacaccagg
     5041 tcgatcatca ccgccgccgg gcggtaaccg cgctcctcca gacgatcgag ttcaaggcgc
     5101 gcgagttcga ggtatttttc accggcctgc gggtcgtcgc gccggtacgg cccgcgatag
     5161 agatttggcg gttcgattac cgccagccag tcggggcgct cggcggccgg gtaactgtcg
     5221 gtcgacattc gccgggtcag ttcggagacg ccgtgatagg acgcctcggt gaccaccagt
     5281 ccgtagttac cggtgacctg gcgggcgatc tgtgccgcga ggtcgttggc ctcgctgccg
     5341 gtacaggtga atacgcaggt atcgagccgg ccgggcaggc gctcgaccag catttcgccg
     5401 agttcgacga tgcctgcgtg caggtagcgg gtatgggtat tcagggtcgc ggcctgccgg
     5461 cacagggcgt cgaccacgcg cgggtggcag tggccgaccg agaccacgtt gttgtagcaa
     5521 tccaggtact ggcggccgtc ggcatcccac agtgccgtgc cggcgccgcg caccaggtgc
     5581 agcgggcgat cgaaaaagtt ctgataggtt ggtccgagcg tattgcgccg gcgctcgatc
     5641 agttggtcgt cgttcattga ctttgccatg gcctcgccta ggggtcgatg cgaattcggg
     5701 tttcgccgcc cagcagcggt ttgctcaact cgatacgcat accctcggcg gcgcacaggc
     5761 tatgggtcgg cgcgcgccgc gatttgaggt atctctcgtt ctccagctgg atctcgtcga
     5821 gttcggaatc gctgcgctcg ggatcgtgat ggaacatcac cagcgcgccg acctcggcgt
     5881 cggtcgccag ctgccgcacc tgcgagatca gcgtgtggcc ccagccgtgc ttgtgcggca
     5941 tgtcgtcctc ggtgtactgc gcataatgaa tcagcaggtc tacgccgtgc agttaattga
     6001 cccattggtc gtaactggtg gtgggcgggt agggcggttc gagttcgttg tcggtgacat
     6061 aggcgacgct gatgccgttt tcgctgatcc ggtaggcgac gccgccgccg ggatgattga
     6121 ggccgcgctt gaccacgttg atcccctcgc gctcgtacaa ttcgctctcg acgcccttga
     6181 acttggcaat attgttggac ggcagatcct ctgcccggac cgggaaatgg gtgccgtcca
     6241 tctgttcgaa cagcgtactg agcagcttgc gcccggactc gacgctgatg tagacgttga
     6301 tgtcgcgatc cggctggtag atcggttcga agaacgggta cccttgaatg tgatcccagt
     6361 ggccgtgcga cagcaggatg ttggcgggcg tcgattttcg cgccagcttc tgtcccagca
     6421 gtcgaatacc ggtgccggcg tcgagaatca ggtcgttgcc ggtctccagc tcgatatgca
     6481 cgcaggaggt gttgccgccg tatttcgcgg tctgcggccc cggcactgcg gtcgagcccc
     6541 ttaccccgta aaaagtaatg ttcatgccgc ggtttcgcta gcccgacgtc ccggtggttg
     6601 gcgtttcctc gacctcatcg gccaaatccc tctcgtccgg gttcaccagt accagtcccg
     6661 ccagcatgac gattaccgat atccacaccc agaccgtatg ctgttcggag aaggcaataa
     6721 tacccc
//
LOCUS       DATCZV010000519         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_625267, whole genome shotgun sequence.
ACCESSION   DATCZV010000519 DATCZV010000000
VERSION     DATCZV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625267"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VIW27_11780"
     CDS             complement(<1..355)
                     /locus_tag="VIW27_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740398.1"
                     /translation="MTHSNRNRAASGRIARRAIFAACLFGALQSPLLADAQTDGERGI
                     EAYQQGRLIEAMQLLESAAQGGYAPAQVLLAFIHDAAENDSEAFEWYQRAAEAGDAAG
                     IFGLGSMYAKGEGVER"
     gene            complement(336..1703)
                     /locus_tag="VIW27_11785"
     CDS             complement(336..1703)
                     /locus_tag="VIW27_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5740399.1"
                     /translation="MRALQLLIIDDEPAIRQILSNAAKNAGHSVSVAANGEEALERLG
                     KGDIDVAVCDIRMPDMTGIEVVEKARSQGLDTIFLMMTAYASVSTAIEAMRAGAYDYT
                     IKPLRNDDFLNRIARLADIIDLKSENKVLRELVKNQEDDVCEPKSSAMQAIDRLVLKV
                     APTDSTVLITGPSGSGKGVIAKMIHQNSLRANRAFVPINCGAIPENLLESEFFGHTKG
                     AFTGASKAKRGLFVEADQGTIFLDEIGELPLNLQVKLLHVIEDQHVRAVGSEQVRKIN
                     VRIIAATNQDLGEMVRRGDFREDLYFRLNVFHVPIPALVERKEDLPLLLRHFIRKESA
                     KMGLQEDFEVTSEAMQCLLAYDWPGNVREVQNVVARALILAEDGVIQLTDLPQGVVGL
                     YDEATDVPQNREDTLKQQLRNYELALIRSAIDEAHGDRKLAAKRLGIGVSSLYRKLEN
                     DTQQS"
     gene            complement(1774..>2217)
                     /locus_tag="VIW27_11790"
     CDS             complement(1774..>2217)
                     /locus_tag="VIW27_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5740400.1"
                     /translation="VRLCHYDNRWSGIEIELDLDSRLPAWYASENQFKQVCMHVLENA
                     LDALQDRQDARLRLQTRLDTEHGIVISFEDNGNGVDEDKLQTIFEPFYSTKAVGGGTG
                     LGLAICLAIVESFDGAIRAEQACGGGLRIVIELPLGNSAHPEIAA"
BASE COUNT          356 a          717 c          687 g          457 t
ORIGIN      
        1 cacgctcgac gccttcgccc ttggcataca tgctgccgag cccgaaaatg cccgccgcgt
       61 cgcccgcctc cgcggctcgc tggtaccatt cgaatgcctc gctgtcgttc tccgcggcgt
      121 cgtggatgaa ggccagcaac acctgcgccg gcgcataacc gccctgcgcc gcgctttcga
      181 gcaattgcat ggcctcgatc agccggccct gctggtaggc ttcgatgccg cgctcgccgt
      241 cggtttgcgc gtcggccagc agcggcgatt gcagcgcgcc gaacaggcag gcggcgaaga
      301 tcgcgcgcct cgcgatacgg ccagaggccg cccggttacg attgctgtgt gtcattttcg
      361 agtttcctgt agaggctcga aaccccgatg ccgagccgtt tggcggccag cttgcgatcc
      421 ccgtgagcct cgtcgatggc ggacctgatc agtgccagtt cgtagttgcg cagttgctgc
      481 ttgagcgtgt cttcgcggtt ttgcgggaca tccgtggctt cgtcgtacag gccgacgacg
      541 ccctgcggca gatccgtcag ctggatgacg ccgtcctcgg ccaggatcag cgctctcgcg
      601 accacgttct gcacttcgcg cacgttgccg ggccagtcat aggccagcag gcactgcatc
      661 gcctcggagg tgacctcgaa atcctcttgc aggcccatct tggcgctctc cttgcgaatg
      721 aaatgccgca atagcagcgg caggtcttcc ttgcgttcca cgagtgccgg gatcggcacg
      781 tggaagacgt tgaggcgaaa gtagagatcc tcgcgaaaat ccccgcgcct gaccatttcg
      841 ccaagatcct ggttggtggc ggcgatgatg cgcacattga ttttcctgac ctgctcgctg
      901 cctaccgccc ggacgtgctg atcctcgatg acgtgcagca gcttgacctg caggttgagc
      961 ggcagctcgc cgatttcgtc gaggaaaatg gtgccctggt cggcttcgac gaacaggccg
     1021 cgcttggcct tgctggcacc ggtgaacgcg cccttggtat ggccgaagaa ctcgctttcg
     1081 agcaggttct ccgggatcgc tccgcagttg atcggtacga aggcacggtt ggcccgcagg
     1141 ctgttctggt ggatcatctt cgcgatcacg cccttgccgg agccgctcgg cccggtaatc
     1201 agcacggtgc tgtcggtggg cgcgaccttg agtaccaggc ggtcaatggc ctgcatcgcc
     1261 gacgatttcg gctcgcagac gtcgtcttcc tggttcttga ccagctcgcg cagcaccttg
     1321 ttttccgact tcaggtcgat gatgtcggcg agtcgcgcga tacggttcag aaaatcgtcg
     1381 ttgcgcagcg gcttgatggt gtagtcgtag gcgccggcgc gcatcgcttc gatcgcggta
     1441 ctcaccgagg cataggcggt catcatcagg aagatcgtat ccagcccctg gctgcgggcc
     1501 ttctcgacga cctcgatacc ggtcatgtcg ggcatgcgga tgtcgcacac ggcaacgtcg
     1561 atatcgccct tgccgagccg ctccagcgcc tcctcgccat tggcggcgac cgacaccgaa
     1621 tggccggcat tcttggccgc gttggacagg atctgccgaa tcgcgggctc gtcgtcgatg
     1681 atcagcagct gcagcgctcg catctttact ataccgccgc gcgtaacggc gcacgccgcc
     1741 tggccggttt cggccgcaac cccgaggctg gcgtcacgcg gcgatctccg ggtgtgcgga
     1801 atttcccagc ggcaactcga tgacgattct caagccgccc ccgcaagcct gttcggcacg
     1861 aatcgcgccg tcgaaagact cgacgatcgc caggcagatc gcgagcccga gcccggttcc
     1921 cccgccgacc gccttggtcg agtagaaagg ttcgaaaatc gtttgcagtt tgtcttcatc
     1981 gacgccgttg ccattgtcct cgaacgatat aacgatacca tgctccgtgt cgagtcgggt
     2041 ttgcagccgc aggcgtgcgt cctgccggtc ttgcagcgcg tccagcgcgt tttcgagcac
     2101 gtgcatgcag acctgcttga actggttctc ggaggcatac caggccggca gccgggagtc
     2161 caggtcgagt tcgatttcga tgccggacca gcggttgtcg tagtgacaca gcctgac
//
LOCUS       DATCZV010000520         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_412824, whole genome shotgun sequence.
ACCESSION   DATCZV010000520 DATCZV010000000
VERSION     DATCZV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412824"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VIW27_11795"
     CDS             complement(<1..322)
                     /locus_tag="VIW27_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740401.1"
                     /translation="MKDLRETAINRLTAARTRLIMEFPFLGVLALRLPLQAADPAWCP
                     TVATDARFLYYNTAWIISLKEYEVRFAIAHEAMHCALAHFVRRGARNRQRWNLACDLA
                     VNGIL"
     gene            complement(334..1336)
                     /locus_tag="VIW27_11800"
                     /pseudo
     CDS             complement(334..1336)
                     /locus_tag="VIW27_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1753..1829
                     /locus_tag="VIW27_11805"
     tRNA            1753..1829
                     /locus_tag="VIW27_11805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Val,seq:gac)
     gene            2149..>2499
                     /locus_tag="VIW27_11810"
     CDS             2149..>2499
                     /locus_tag="VIW27_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY5740402.1"
                     /translation="MPQITLPDGSKREFEQPVSVMEIAAAIGPGLAKAMLAGKVDGRL
                     VDAAHVVDRDAEVAIVTGRDDEALELLRHDAAHVMAQAVQELYPGTQVTIGPAIEDGY
                     YYDFARDEPFTPEDL"
BASE COUNT          463 a          763 c          813 g          460 t
ORIGIN      
        1 tcaggatgcc gttgaccgcc aggtcacagg ccaggttcca gcgctggcgg ttgcgcgcac
       61 cgcggcgcac gaaatgcgcc agcgcgcaat gcatcgcttc gtgggcaatc gcgaaacgca
      121 cctcgtattc cttcagcgag atgatccacg cggtgttgta atacaggaag cgggcatcgg
      181 tggcgaccgt cgggcaccag gcggggtcgg cggcctgtag cggcaggcgc aaagcgagca
      241 cgccgagaaa cggaaactcc atgatcagcc gcgtgcgcgc cgcggtcaga cggttgatgg
      301 cggtctcgcg aaggtctttc accgagtacc gggctagtcg actaacagac cagcggtttc
      361 ttcagcccac tgcgtgaaac cctgcagctt gaacagctcg ccggcgagaa tacggtgcag
      421 gtcgctgacc agcatcacgc cgatttcacg ttgcgaaaac tggctcgcga aggcaagaat
      481 gttttccagc gcctgcttgc cgctgctggc gccgggctcg agcgttttcg cgcgccgcat
      541 cagcgccgcc gccaccgcgt actgcaggtc gacctcgtcg ggcatctcga cgcgctggcc
      601 gctgaggatg gcgtcgatat cgggcagcct ttcgagatgt tcgaggtaag ccatgaactc
      661 gatgccggcg gcctcgccga cgcaggccac gagggacgca ctcaacagtt ccggctgccc
      721 ggcgaacttt tgcagtgcgc ggtgcgcgaa ctcccaggta cgcggcgacg gaaacgcgat
      781 agggttttcg ctctgctcga actcgaacag cagttcgggt cgaaagcgca agaaaccgat
      841 gacatgctcg ttgatgccct cgcgcagcgc ccagctcacc cagtcctcga gatcgacctc
      901 gatctcgtaa tgggtgaagc ggttcgccag cggcgtcggc atcacgtgcg agacgccgcg
      961 gtcgccctgg cggttgccgg cggcgaagat cgagaatcag ctggtaggcg gccgccgata
     1021 cgctcggcgg tgcggcgttg atttcgtcga gaaacaggat gccccgctcg ccgtgacgtt
     1081 cggcgtgggg cagcagtcgc ggtacggccc attcgacgcc atcctcgatg cggtagggaa
     1141 tgccgcgcag gtcgctaggt tcgatctgcg acagtcgaag gtcgatcatc ggggtggaaa
     1201 atttttcggc cagctcggcg acgatgcgcg attttccgac tccgggcggc ccccacagca
     1261 taaccggggt atgggtgcct ttctcggcgc tcagcagttc ctgctcgaga atcggaatca
     1321 attgagatag tcgcatatcg ggtccctgtc actgtctcga ttatagtcgc tctgtatgag
     1381 cctgccggca gtccgctcgc ggctgcttcg gtgagtccag ttgggtattt tttcatttat
     1441 aaatcaatgc attctggcta tctacggcgc gatcgactgt gccgaatcga gcggctgcag
     1501 cggttttcgg cggctgccgg gggaggcttt gcgaggcggc tttccgactg ccgtggcatc
     1561 ggcttgccgc accgatcgac gcttatcgcg tcgcttgccc acgcgctcac cggccgcggt
     1621 ttcgaggtgg ccgcagcggc gccggccgat aacagccggc ggtgaatcgg ttagcacgat
     1681 cgagtgccga ggaacggttg attagttgaa tattcaaggt agaatagccg gcctgatttt
     1741 agctggttcc atagtcgcgt agctcagttg gttagagcac caccttgaca tggtgggggt
     1801 cggtggttcg aatccactcg cgactaccaa cttcagttgg tcggcgcgag tggccgaggt
     1861 gcggtggttc gacaaaatcg ccgggaggcc gcgaatgcac atccatgtgc tacgcggcac
     1921 ctgctcatcc atgagcaatg cgattttgga cgcgcgcagc gcggccctgg agtgcggccg
     1981 aaggtcgcac ggaagggtgg agggcaggat gcccgacgca atccactcgc gactaccaac
     2041 ttcaccaggc ccgagcgcgg agccggcgaa gccagcgtga acaaagcgcc gtcaaccggc
     2101 acccaaatca tgtggccttt ggtaggggtc accactggag aaatcgcaat gccccagatt
     2161 acgctacctg acggctcgaa acgcgagttc gagcaacccg tatccgtaat ggaaatcgcc
     2221 gccgctatcg gtcccggcct ggcgaaagcc atgctcgccg gcaaggtcga tggcaggctg
     2281 gtcgacgccg cccacgtcgt cgaccgcgat gccgaggtcg cgatcgtcac cggccgcgat
     2341 gacgaggcgc tggaattgct gcgccacgac gccgcgcacg tgatggcgca ggcggtgcag
     2401 gaactctatc ccggcaccca ggtcaccatc ggcccggcga tcgaggacgg ctattattac
     2461 gacttcgcgc gcgacgagcc cttcacgccc gaagacctg
//
LOCUS       DATCZV010000521         4299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_625336, whole genome shotgun sequence.
ACCESSION   DATCZV010000521 DATCZV010000000
VERSION     DATCZV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625336"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VIW27_11815"
     CDS             complement(<1..454)
                     /locus_tag="VIW27_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5740403.1"
                     /translation="MILSLATMFGGAAPAADNVGFVYVSPIGDAGWTYQHDLGRLQVE
                     KETGVTTSYVENVAEGADAERVIREMAKRGDKVVFATSFGYMNYMLKVSKSFPDTAFV
                     HATGYKLGDNMGLVNARFYEGRYLTGVIAGEMTESNVLGYVAAFPIPEV"
     gene            complement(613..1545)
                     /locus_tag="VIW27_11820"
     CDS             complement(613..1545)
                     /locus_tag="VIW27_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740404.1"
                     /translation="MDASIVTSILFATVVAGTPLIIVALGQLVAEKSGVLNLGAEGMM
                     AMGAIAAFAATLHSGSPWLGVLAGMAAGAAMALLFAVVVLSLMGNQVASGLALSILGV
                     GLAAFVGKPYESEVLPTVAAWRIPLLSDIPLLGEALFSHQGLVYFSWALFAGISWFLY
                     RSRPGLVLRAVGESPTSAHAIGYNAIRIRYSATLFGGAMAGIGGAFLSVFYTPLWAEG
                     MVAGRGWIAIALVVFATWRPLRVVLGAYLFGGVMVVQLFVQGSGLEINIPPQLLSALP
                     YLATIVVLVIISRDPNTVRLNAPVSLGKPFRPEG"
     gene            complement(1545..2612)
                     /locus_tag="VIW27_11825"
     CDS             complement(1545..2612)
                     /locus_tag="VIW27_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740405.1"
                     /translation="MLRLERRPEASRAMTYASPLIAVVLMLIGGLLLFAALGKNPLQG
                     FRVFFLYPLSDLYGISELLLKATPLMLCAIGLAIGFRANIWNIGAEGQLIVGAIAASA
                     VALYFYDSAGGPHVLMLMVLAGAVGGMLWAAIPAFLKTRFNTNEILVSLMLVYVAQLL
                     VSWLVHGPMMDPDGFNFPQSRLFEDSALLPLLFDGTRLNIAFLFALGALLAGYVFMHR
                     SFVGFQMQVTGHARDAARYAGFPARRIIWIGLLSGGAMAGLAGMSEVAGPMGQITDHV
                     SNNYGFAAIIVAFVARLNPVGIFFASLLMALLYLGGEQAQQYLNLPSSISSVFQGMLL
                     MFLLGSDVFVNYRIRWRQAVA"
     gene            complement(2599..4161)
                     /locus_tag="VIW27_11830"
     CDS             complement(2599..4161)
                     /locus_tag="VIW27_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740406.1"
                     /translation="MTTAASAKPDFRLQLEGITKVYPSVTANDAVSLAVGKGEIHAVL
                     GENGAGKSTLMKIIYGVTRPDAGQIRWQGEVVRIDNPADARRLGIGMVFQHFSLFETL
                     SVVENVALALRGDTDLEQLADRIKEVSGHYGLPVDPWRSVHALSVGERQRVEIIRCLL
                     QKPRLLIMDEPTSVLTPQAVRKLFATLRQLSAEGCSILYISHKLDEIQELCHSATVLR
                     DGRVTGNCIPSEETPQSMARMMIGRELPHAEDSSAIEPGEVRLRLKGLSKPSSDPFGT
                     DLQAIDLELRAGEILGIAGVSGNGQRELLYALSGEETLGDAQSIVICGQPAGRDSPAR
                     RRAAGMGFVPEERLGRGAVPAMSLIDNALLTAHRAGMLEHGLIRFDRIADFTRRCIER
                     FGVRCGGPQHAASSLSGGNLQKFIIGREILLAPKLLVVAQPTWGVDVGAAAFIRQQLV
                     DLRNAGTAILLVADELEELFEISDRIAVISRGRLSPAIPLKETSVDEIGVWMSGMWEQ
                     QTPARKLDHAQA"
BASE COUNT          822 a         1421 c         1386 g          670 t
ORIGIN      
        1 gcacctccgg gatcgggaac gcggcgacat agccgagcac gttggactcg gtcatctcgc
       61 cggcgatcac gccggtcagg tagcggccct cgtaaaagcg tgcgttgacc agacccatgt
      121 tgtcgccgag cttgtaaccg gtggcgtgca cgaaggcggt atcgggaaaa cttttcgaca
      181 ccttgagcat gtagttcatg tagccgaagc tggtggcgaa gacgaccttg tcgccgcgct
      241 tggccatctc gcgaatcacg cgctcggcgt cggcgccctc ggcgacgttt tcgacatagc
      301 tggtggtgac gccggtttcc ttctcgacct gcagccggcc gagatcgtgc tggtaggtcc
      361 agccggcgtc gccgatcggg ctcacataga cgaagccgac gttgtcggcc gccggcgccg
      421 cgccgccgaa catggtggcc agtgacagga tcatggccgc ggcaagccgc cggttacctc
      481 tggtagtcgc taacattatt caaccctccc cggagtcgtg tttgaaatag ctcaatgcgg
      541 gcctcgcaac ccgcggatta taaacctgta atggcgaatt tccgcgcgag aaattcgtcg
      601 ccgcgccctg cttcatccct cgggcctgaa cggctttccc agcgagaccg gcgcgttcag
      661 ccgcacggtg ttcggatcgc gcgagatgat caccagcacg acgatggtcg cgaggtacgg
      721 cagcgccgac agcaactgcg gcggaatatt gatttccagt cccgagccct gcacgaacag
      781 ctgcaccacc atgacgccgc cgaacaggta ggcgccgagc accacccgca gcggccgcca
      841 ggtcgcgaac accaccagcg cgatcgcgat ccagccgcgc ccggcgacca tcccctcggc
      901 ccatagcggg gtatagaaaa ccgacagaaa ggcgccgccg atgccggcca tcgcaccgcc
      961 gaacagcgtc gccgagtagc gaatgcggat cgcattatag ccgatcgcgt gcgccgatgt
     1021 cggcgactcg ccgaccgcgc gcaataccag tccgggacgg ctgcggtaga ggaaccagct
     1081 gatgccggcg aacagcgccc aggaaaaata caccagcccc tggtggctga acaacgcctc
     1141 gccgagcagc ggaatatccg acagcagcgg aatgcgccag gcagcgaccg tcggcagcac
     1201 ctcggactcg tagggcttgc cgacgaaagc cgccaggccg acgccgagaa tcgacagcgc
     1261 caggccgctg gcgacctggt tgcccatcag cgacagcacc actaccgcga acagcagcgc
     1321 catcgcggcg ccggcggcca tgccggcgag cacgccgagc cacggactgc cgctgtgcag
     1381 cgtggccgcg aacgcggcga tcgcacccat cgccatcatg ccctcggcgc cgaggttgag
     1441 tacgccggat ttctccgcga ccagctgccc gagggcgacg atgatcagcg gcgtgccggc
     1501 gaccacggtg gcgaacagaa tcgaggtgac gatcgacgcg tccatcaggc gacggcctgc
     1561 cgccagcgaa tgcggtaatt gacgaacacg tcggaaccga gcaggaacat cagcagcatg
     1621 ccctggaaca cgctggagat cgacgacggc aggttcagat actgctgcgc ctgctcgccg
     1681 ccgaggtaga gcagcgccat cagcaggctg gcgaaaaaga tgcctaccgg attgaggcgc
     1741 gctacgaagg cgacgatgat cgccgcgaag ccgtagttgt tcgatacgtg atcggtgatc
     1801 tgccccatcg gcccggcgac ctcggacatg ccggccagcc cggccatcgc gccgcccgac
     1861 agcaggccga tccagatgat acgccgtgcc ggaaacccgg catagcgcgc cgcgtccctg
     1921 gcgtggccgg tgacctgcat ctggaagccg acgaagctgc ggtgcatgaa cacgtaaccg
     1981 gcgagcagtg cgccgagcgc gaacaggaag gcgatattga gccgcgtgcc gtcgaacagc
     2041 agcggcaaca gcgccgaatc ctcgaacagc cgcgactgcg gaaagttgaa gccgtcggga
     2101 tccatcatcg gcccgtgcac cagccacgag accagcagct gggcgacgta gaccagcatc
     2161 agcgagacca ggatctcgtt ggtattgaag cgggttttga gaaaggccgg aatcgccgcc
     2221 cacagcatgc cgccgacggc gccggcgagc accatcagca tcagtacgtg ggggccgccc
     2281 gcggaatcgt aaaagtaaag cgccaccgcg ctggcggcga tcgcgccgac gatcagctgg
     2341 ccctcggcgc cgatgttcca gatgttggcg cgaaagccga tcgccaggcc gatcgcgcac
     2401 agcatcagcg gcgtcgcctt gagcaacagc tcggagatgc cgtagaggtc gctgagcggg
     2461 tagaggaaaa atactcgaaa cccctgcagc gggtttttgc cgagcgccgc gaacagcagc
     2521 agcccgccga tcagcatcag caccacggcg atcagcggcg acgcgtaggt catcgcccgc
     2581 gaggcctcgg gacgccgctc aagcctgagc atggtcgagt ttccgtgccg gcgtttgctg
     2641 ctcccacatg ccgctcatcc agacgccgat ctcgtcgacg ctggtttcct tgagcggtat
     2701 cgccggcgac aggcggcccc tggagatgac tgcgatgcgg tccgaaatct cgaacagttc
     2761 ctcgagttca tcggcaacca gcaggatcgc ggtgccggcg ttgcgcagat cgaccagctg
     2821 ctggcgaatg aacgcggcgg cgccgacgtc gaccccccag gtcggctgcg cgaccaccag
     2881 cagtttcggc gccagcagga tttcgcggcc gatgatgaat ttctgcaggt tgccgccgga
     2941 cagcgaactg gcggcgtgct gcgggccgcc gcacctgaca ccgaagcgct cgatacagcg
     3001 cctggtgaag tccgcgatgc gatcgaaacg gatcagtcca tgctcgagca tgccggcgcg
     3061 gtgcgcggtc agcagcgcgt tgtcgatcag cgacatggcc ggcaccgcgc cgcggccgag
     3121 gcgttcctcg ggcacgaaac ccatgccggc ggcgcgccgc cgggccggcg aatcgcgccc
     3181 cgccggctgg ccgcagatga cgatcgattg cgcatcgccg agcgtttcct cgccggacag
     3241 cgcgtacagc aactcgcgct ggccgttgcc ggagaccccg gcgatgccga ggatttcgcc
     3301 ggccctgagc tcgagatcga tggcctgcag atcggtgccg aagggatcgc tcgacggttt
     3361 cgacaggccc ttcaggcgca gccgaacctc gccgggttcg atcgcggaac tgtcctcggc
     3421 atgcggcaac tccctgccga tcatcattct tgccatcgac tgcggggttt cctcggacgg
     3481 aatgcagttg ccggtgacgc ggccgtcgcg caacacggtc gccgaatggc aaagttcctg
     3541 gatttcgtcg agcttgtgac tgatgtacag gatgctacag ccctcggcgg aaagttggcg
     3601 cagggtggcg aacagcttgc gcaccgcctg cggggtcagc accgaggtcg gctcgtccat
     3661 gatcagcagg cgcggttttt gcagcaggca gcgaatgatt tcgacccttt gccgctcgcc
     3721 gaccgacagc gcgtgcacgc tgcgccacgg gtcgaccggc aggccgtaat ggccggaaac
     3781 ctccttgatg cgatccgcga gctgctcgag atcggtatcg ccgcgcagcg cgagggcgac
     3841 gttttcgacc acgctcaggg tttcgaacag cgaaaaatgc tggaatacca tgccgatgcc
     3901 gagccggcgg gcgtcggcag gattgtcgat gcgcaccact tcgccctgcc acctgatctg
     3961 cccggcatcg gggcgggtga cgccgtagat gattttcatc agcgtcgact tgccggcccc
     4021 gttctcgccg agcaccgcgt ggatctcgcc cttgccgacg gcgagcgaaa ccgcgtcgtt
     4081 ggcggtcacc gatggataaa ccttggtaat accctcgagt tgcaggcgaa aatccggttt
     4141 cgcactcgct gcggtcgtca tgcttttacc ctggcgaaga cttgttatcg ttgcgaagca
     4201 gcaggtttcc tgctgagccc gatcgtatac cccaaaattt tcgccggcaa ccgcaatcgt
     4261 cgccacgggc ggctgctccg attccggcgg gcaaaaccc
//
LOCUS       DATCZV010000522         3729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_254797, whole genome shotgun sequence.
ACCESSION   DATCZV010000522 DATCZV010000000
VERSION     DATCZV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254797"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1358)
                     /gene="miaB"
                     /locus_tag="VIW27_11835"
     CDS             complement(18..1358)
                     /gene="miaB"
                     /locus_tag="VIW27_11835"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY5740407.1"
                     /translation="MPKKLFIKTMGCQMNEYDSEKMADVLRESHGFSLTENPEEADLL
                     LVNTCSIREKAQEKVFSELGRWRSWKEQRPDLLIGVGGCVASQEGTGINRRAPFVDMV
                     FGPQTLHRLPQMVDEARRTGAPVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
                     CTFCVVPYTRGEEISRPLDDVVTEAVNLAAQGVREVNLLGQNVNAYLGPMHDGQLADL
                     ATLIYYIAAIDGIDRIRFTTSHPREFTDSLIQAYAEIPELANFLHLPVQHGSDRILSR
                     MKRGHTALEYKQKIRKLREVRPDISISSDFIVGFPGESDSDFDATMQLIADVGFDQSF
                     SFVYSARPGTPAASFTDQTPLAVKKERLRELQERIRDQAMEISRAMVGTVQSVLVEKP
                     SKKNPRQLTGRTENMRWVNFDADPSYIGQFADVLITEALPNSLRGRLTGKRAAA"
     gene            complement(1380..1814)
                     /gene="ssb"
                     /locus_tag="VIW27_11840"
     CDS             complement(1380..1814)
                     /gene="ssb"
                     /locus_tag="VIW27_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5740408.1"
                     /translation="MARGVNKVILVGNLGNDPDTRYMPSGSAVTNLSVATNESWKDKQ
                     TGEQKDRTEWHKVAMFGRLAEIAAEYLRKGSQVYIEGKLRTRKWQDQNGNDRWTTEII
                     ADEMQMLGGRTGAGAPAMGDSPPPAAPPPEPSPDEFDDDIPF"
     gene            complement(1914..3092)
                     /locus_tag="VIW27_11845"
     CDS             complement(1914..3092)
                     /locus_tag="VIW27_11845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740409.1"
                     /translation="MLKQISQFFAVMLPVERRAVSVVALMAMCRMFGLFALLPVLAIF
                     AADLKGATPGLIGLAVGAYGLTQAALQIPFGALSDRIGRVPVILFGLALFAAGSIIAA
                     RSDSIYVVIAGRLLQGAGAISATFTALLADATREEIRTRSMAVLGISIGSSFLLALVI
                     GPAIAAAAGVRSLFWLAAILAAVAALLLTMMPRGIERPQRRADRSISAAFRPDLLRLD
                     LYIFVLHALLTASFVALPFLLRNELQVAVADQWQIYTAALLVSLLGTVPLIIADERKG
                     KSSTVTTAIVLLMAGQAVLAFAGASLAIVGFALAVFFAGFNFLEAGLPARLSMQAAGD
                     LRGASLGVFSSSQFLGAFAGGLIGGRFLAGGDPSAVFLVCALLAALWLLVHALAAGKD
                     "
     gene            3177..>3729
                     /locus_tag="VIW27_11850"
     CDS             3177..>3729
                     /locus_tag="VIW27_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5740410.1"
                     /translation="MKSIDIRGARTHNLKNLDLSLPRDKLIVFTGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLSMMEKPDVDHIDGLSPAISIEQKSTSHNPRSTVGTVT
                     EIYDYLRLLYARAGIPRCPDHGVPLEAQTVSQMVDQVLAMPEGSRLLLLAPVVIERKG
                     EHVQLMQDLQAQVIVNFGDRADCR"
BASE COUNT          741 a         1172 c         1117 g          699 t
ORIGIN      
        1 aatacgaatc ggctccgtca ggcggcagcc cgcttgcccg taagccggcc acgcagggag
       61 tttggcagcg cctcggtgat caggacgtcc gcgaactgac cgatatagga cggatcagca
      121 tcgaaattga cccagcgcat gttctccgta cgccccgtaa gctgcctcgg gttttttttc
      181 gacggttttt cgaccagcac ggattgcacc gtaccgacca tggctcgact gatttccatc
      241 gcctggtcac ggatgcgctc ctgcaattcg cggagccgtt cctttttcac cgccagcggc
      301 gtctgatcgg tgaaactggc agcaggcgtg cccggtcgag cgctgtagac gaagctgaac
      361 gactggtcga agccgacatc ggcgatgagc tgcatcgtcg cgtcgaaatc gctgtcgctc
      421 tcgcccggaa aaccaacgat aaagtcggat gaaatcgaga tgtccggcct gacctcccgg
      481 agtttgcgta tcttctgctt gtactcgagc gccgtgtgac cgcgtttcat gcgcgacagg
      541 atacggtcgg acccgtgttg caccggcaaa tgcaggaaat tggccagttc cggaatctcg
      601 gcataggcct ggataaggct gtcggtgaac tcgcgcgggt gcgaggtcgt aaaacgaata
      661 cggtcgatgc catctattgc ggcgatgtaa taaataagtg tcgcaaggtc cgcgagctga
      721 ccatcgtgca tcggcccgag gtaagcattg acgttttgac ccagtagatt aacctcgcgc
      781 acgccctgag cggcgagatt gactgcctcc gttaccacgt catcgagcgg gcggcttatt
      841 tcctcgccgc gcgtatacgg cacgacacag aaggtgcagt acttactgca tccttccatg
      901 atggatacga aagcagtcgg tccctccgca cgcggttccg gcaggcgatc gaatttctca
      961 atctccggaa acgaaacgtc gaccaccggt gcgcccgttc gccgcgcttc gtcgaccatt
     1021 tgcggcaatc ggtgcagggt ctgtggcccg aataccatgt cgacgaatgg cgctcgccta
     1081 ttgatgcccg tgccttcctg gctggccacg caaccgccta cgccgatcag caggtccggc
     1141 cgctgctctt tccacgaccg ccagcggccg agttccgaaa aaaccttttc ctgcgctttc
     1201 tcgcggatcg agcaggtgtt gaccagcaac aggtctgcct cctcaggatt ctctgtcagc
     1261 gaaaacccat gcgattcccg gaggacgtcc gccatcttct ccgagtcgta ctcgttcatc
     1321 tggcagccca tcgtcttgat gaaaagtttt ttcggcattg cttgcgaaac ttcgccgctt
     1381 tagaagggta tgtcgtcgtc gaattcgtct ggagaaggct cgggcggggg tgccgccggc
     1441 ggcggtgaat cgcccatcgc gggtgcgccg gcaccggtcc ggccgcccag catctgcatc
     1501 tcgtcggcaa tgatttccgt cgtccagcga tcgttgccgt tctggtcctg ccattttcgg
     1561 gtgcgcagct tgccctcgat atacacctgg gaccctttcc tcaggtattc ggccgcgatc
     1621 tcggccaggc ggccgaacat cgcaaccttg tgccactctg tacggtcctt ttgctcgccg
     1681 gtctgcttgt ccttccagga ttcgttggtc gccacgctca ggttggtgac agccgaaccg
     1741 ctgggcatgt agcgggtatc cgggtcgttc ccgagattac cgacgaggat tactttgttg
     1801 actccacggg ccattgtggt ccccttgtga tgattttagc cccggttggg gggtacgtat
     1861 tgtaggcgct acccggtcaa ttgccagcct gaaatcaggc agataaggga gtttcagtct
     1921 ttgccggcag caagcgcgtg caccaggagc cacagcgcgg ccaggagcgc gcagaccagg
     1981 aaaacggcgg acggatcgcc gcccgcgagg aagcggcccc cgatcaggcc gccggcaaat
     2041 gcgccgagga actgcgagct cgagaaaacg cccagcgacg cgccgcgcag gtcccccgcg
     2101 gcctgcattg agagcctggc cggcaacccg gcttccagga aattgaagcc cgcgaaaaat
     2161 accgccagcg cgaaaccgac aatggcaagg ctcgcgcccg caaacgcgag gacggcctgc
     2221 cctgccatga gcaagacgat tgccgtcgta accgtcgacg acttgccctt gcgttcatcc
     2281 gcgatgatca gcggcaccgt gccgagcaag gacaccagga gcgcagccgt gtaaatctgc
     2341 cactggtcgg cgaccgccac ctgcagctcg ttgcgcaaga ggaagggcaa agccacgaag
     2401 cttgcggtca ggagagcgtg caaaacgaaa atgtacaggt cgagccgcaa caggtccggc
     2461 cggaacgccg cgctgatact ccgatccgca cgccgctgcg gccgctcgat accgcgcggc
     2521 atcatggtca ggagcaaagc ggcaacggcg gcgaggatcg ctgccagcca aaaaagcgaa
     2581 cgtacgccgg cggccgccgc gatcgccggg ccgatgacga gtgccagcag gaacgagctg
     2641 ccgatcgaaa tgcccagcac cgccatcgat cgcgtccgta tttcctcgcg ggtggcatcg
     2701 gcaagcagtg cggtgaaggt agccgagatc gcgccggcgc cctgcagcag ccggccggcg
     2761 ataaccacgt agatgctgtc gctgcgcgcc gctatgatgc tgcccgcggc aaacagtgcg
     2821 agtccgaaaa gtatgaccgg caccctgcct atgcggtcgg acagggcgcc gaagggaatc
     2881 tgcaacgcgg cctgtgtcag gccgtaagcg cctacggcca ggccgatcag ccccggcgta
     2941 gcgcccttga gatctgccgc gaatatcgcg agcacgggca aaagcgcgaa caaaccgaac
     3001 atgcggcaca tcgccatcag cgcaactacc gaaacggcgc gccgctcgac cggcaacatg
     3061 actgcaaaaa actggcttat ttgcttgagc ataattagtt gaaccaaccc cgcctttgcg
     3121 ccgcagttgc gcggcggcgt atattagcag gttgtttgcc gttttctctt cggaccatga
     3181 aatccatcga cattcgcggc gcgcgaacgc acaacctgaa aaatctcgac ctgagcctgc
     3241 cgcgggacaa gctgatcgtg ttcaccggtc tttccggctc cggcaaatca tcgcttgcgt
     3301 tcgacaccat ttatgccgaa ggccagcgac gctacgtcga gtcgctgtcg gcctacgcgc
     3361 ggcaattcct gtcgatgatg gaaaaaccgg acgtcgacca catcgacgga ctgtcgcccg
     3421 ccatctcgat cgagcagaag tcgacctcgc ataatccgcg ctcgacagtc ggcacggtca
     3481 ccgagatcta cgattacctg cgcctgctgt acgcccgggc cggcattccg cgctgccccg
     3541 atcacggcgt gccgctggag gcacagacgg tcagccagat ggtcgaccag gtgctggcga
     3601 tgcctgaagg cagccgcctg ctgttgctgg cgccggtcgt catcgagcgc aagggcgaac
     3661 acgtacagct gatgcaggac ctgcaggcgc aggtaatcgt aaatttcggt gaccgtgccg
     3721 actgtcgat
//
LOCUS       DATCZV010000523         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_573836, whole genome shotgun sequence.
ACCESSION   DATCZV010000523 DATCZV010000000
VERSION     DATCZV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573836"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VIW27_11855"
     CDS             <1..404
                     /locus_tag="VIW27_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5740411.1"
                     /translation="QIMQDPDEVVEVLSHLGVMGLSLSIDDFGTGQASLTYLKELPVE
                     KLKIDQSFVLDLASNPDDQLIVKATIELAHTLDLKVVAEGVETLAVCEVLTEMNCDQV
                     QGYYISRPLEAEQIADWLATSIKPELVRKGR"
     gene            complement(498..1169)
                     /gene="radC"
                     /locus_tag="VIW27_11860"
     CDS             complement(498..1169)
                     /gene="radC"
                     /locus_tag="VIW27_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY5740412.1"
                     /translation="MAISHWPSAERPREKLITRGAKSLSDAELLAIFLRSGVAGSSAV
                     DLARELLQDFGSLRGVMAADASRFCARKGLGPVKFAQLQAALEIARRHFAESLSRGDC
                     LTRPAQTMRYLRARLRDYDHEVFACLMLDNRNRVIALRELFRGTIDGASVYPREVVKQ
                     ALADNAAAVIFAHNHPSGVSEPSQADIRITERLQQALALVDIRVLDHVIIGDGVTCLS
                     ERGLI"
     gene            complement(1318..>2498)
                     /locus_tag="VIW27_11865"
     CDS             complement(1318..>2498)
                     /locus_tag="VIW27_11865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5740413.1"
                     /translation="ESRLLTLEGPIMTATQVHPIDSMAAKDWQQLAAELGTEFGNRAA
                     THDSENRFVADNYESLRAHGLFSLMIPEQLGGGGADYATLCNLVREFGRHCGSTALSF
                     SMHSHPVALNVFKHLRGDDKATATLRRIADQQLVIAGTGANDWLESSGEMTPVDGGYR
                     VTARKHFVSGGPGAQVFVSSATLAGEDGSEVLHFAIPFASAGVNRHDNWNTLGMRGTG
                     SNDISLDDVFVPEAAIVARRPAGEWHPMWNVILPVAMPIIMSAYMGIADAALELARAA
                     AAKRADDLADAFGEAQNRHTAAAIAHADMVRLSDNYGFTPTTQLSSAILTRKTLLADA
                     ARDSVELAVEIVGGAGFFKGHPMERLLRDVRASHFHPLPYRRQYRFCGRVGLGLDPIG
                     "
BASE COUNT          432 a          769 c          863 g          434 t
ORIGIN      
        1 gccagatcat gcaggatccc gacgaagtcg tcgaagtcct gtcgcacctg ggcgtgatgg
       61 gcctgtcgct gtcgatcgac gacttcggta ctggccaggc gtcgctgacc tacctcaagg
      121 aattgccggt cgaaaagctc aagatcgacc agtccttcgt gctggatctg gcgagcaacc
      181 ccgacgacca gttgatcgtc aaggcgacca tcgagctggc gcacacgctg gacctgaagg
      241 tggtcgccga gggtgtcgag acactcgccg tctgcgaggt gctgaccgag atgaattgcg
      301 accaggtgca gggttactat atcagccggc cactcgaggc tgagcagata gccgactggc
      361 tggcgacctc gatcaagccc gagctggtac gcaaaggacg ctgatacggc ccgcgtgccg
      421 gcggctcgga tgccgcgaaa ccggcgtgtt cgccgccagc cgcggcgatt cggtcggccc
      481 gtcactcgcg cggccgttca gatcaggcca cgctccgaca ggcaggtaac gccgtcaccg
      541 atgatgacgt ggtcgagtac gcgaatatcg accagcgcca gcgcctgctg caggcgctcg
      601 gtgatgcgaa tatccgcctg gctgggttcg gatacgcccg acggatggtt atgcgcgaag
      661 atgaccgccg cggcgttatc ggccagcgcc tgcttgacca cctcgcgtgg gtagacgctg
      721 gcgccgtcga tggtgccgcg aaacagttcg cgtagcgcga tcacgcggtt gcggttgtcg
      781 agcatcaggc aggcgaacac ttcgtggtcg taatcgcgca gccgcgcgcg caggtagcgc
      841 atggtttgcg ccggccgtgt caggcaatca ccgcggctga gcgattcggc gaagtggcgg
      901 cgcgcgatct cgagcgcggc ctgcaattgc gcgaacttga ccggccccag gcccttgcgc
      961 gcgcagaagc gcgacgcatc ggcggccatc acgccgcgca gcgaaccgaa gtcctgcagc
     1021 agctcgcgtg ccagatcgac cgcggaactt ccggcgacgc cgctgcgcag gaaaatggcg
     1081 agcagctcgg cgtccgacag cgattttgcg ccgcgggtga tgagtttttc acggggtcgt
     1141 tcggcactgg gccagtggga gatagccatg ggtattcctt tacctgctga gctggccttc
     1201 gaagttaaag caaattcacg ccggtagcaa gccgcacggc cgaggagtag ggggccgaat
     1261 acgaatgggc caccctgccg ctgccggtaa acggtcgccg cggggtgacc cgtgagctca
     1321 gccgatcggg tcgaggccga ggccgacgcg gccgcagaag cggtattggc ggcggtaggg
     1381 cagcgggtga aagtgcgagg cgcgcacgtc gcgcagcaat cgttccatcg ggtggccctt
     1441 gaaaaaacct gcgccgccga cgatttcgac agccagttcg acgctgtcgc gggcggcgtc
     1501 ggcgagcagt gtcttgcgcg tcaggatagc gctcgacagc tgggtggtcg gcgtgaagcc
     1561 gtagttgtcg ctgagtcgca ccatgtcggc gtgggcgatc gccgcggcgg tgtgccggtt
     1621 ttgcgcttcg ccaaacgcgt cggcgaggtc gtcggcacgc tttgccgccg ccgcgcgcgc
     1681 cagttccagc gccgcgtcgg cgatgcccat gtaggccgac atgatgatcg gcatcgcgac
     1741 cggcagaata acattccaca tcgggtgcca ttcgccagcc gggcggcgcg cgacgatcgc
     1801 cgcttccgga acgaagacgt cgtccagcga gatgtcgttg gagccggtgc cgcgcatgcc
     1861 gagcgtattc cagttgtcgt ggcggttgac gccggcgctg gcgaacggaa tcgcgaaatg
     1921 cagcacctcg ctgccgtcct cgccggccag tgtcgcgcta ctgacgaaca cctgtgcgcc
     1981 ggggccgccg ctaacgaaat gcttgcgcgc ggtgactcga tagccgccgt cgacgggcgt
     2041 catttcgccg gacgactcca gccagtcgtt ggcgccggta ccggcgatca ccagctgctg
     2101 gtcggcgatt ctgcgcagcg tggcggtggc cttgtcgtcg ccgcgtaagt gcttgaacac
     2161 gtttagcgcg accgggtgag agtgcatcga aaagcttagc gcggtcgaac cgcagtggcg
     2221 gccgaattcg cgcaccaggt tgcacagcgt ggcgtaatcg gcgccaccgc caccgagctg
     2281 ctcgggaatc atcagtgaga acaagccatg cgcacgcagg ctttcgtaat tatcggcgac
     2341 gaagcgattt tcgctgtcat gcgtggcggc gcggttgccg aattccgtgc ccagctcggc
     2401 ggccagttgc tgccagtcct tagcggccat ggaatcgatt gggtgaactt gtgtggcggt
     2461 catgatcggg ccctccaggg tcagtaaacg ggattctt
//
LOCUS       DATCZV010000524         5433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_307067, whole genome shotgun sequence.
ACCESSION   DATCZV010000524 DATCZV010000000
VERSION     DATCZV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307067"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VIW27_11870"
     CDS             <1..1540
                     /locus_tag="VIW27_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5740414.1"
                     /translation="VELEPTDENRAILNVAFAERKKSLEFENNAPQLRLSDLNLQRVF
                     SAAYKKYQDRPIGSVKLENVGGTEFGNLKLSFQIKEFMDFPSAVEIPRIAANETLEIP
                     IKATFNNRILEVDEDTGVQVEVRLSYLRDGQNDDISLTQPMTIYGKNAIVWKDPHMIG
                     SFVTPKDDTLRNYVRQVVNSFQPDPGPLNDKLVAAMSYFSSLTASGTSYIVDPNTPFT
                     ELRDDQIDYVQFPRETLRLKSGDCDDLSVLISAGLENLGIRTAFVEVPGHLFLMFDTG
                     LEAGLADLISQDRNLLVVRDDTVWIPLEATMINTDFNEAWAEGARKYQAALAAEQLGI
                     IDLRQAWQSYKPVTLRQANYNIDLPQPQRAASLVNQARKVLLAKSIDRLILPYQTMVA
                     NNPKDLAARLQIAILYTRYGLYADAELAFEALSELAPQSSAVKTNQGNLYFLQGNYDR
                     AIENYRRAADLDTEDGGILINLSMAHYKAGDLTKARTSYQNALELNASLQQEYDAYSK
                     LLSQ"
     gene            1559..2095
                     /locus_tag="VIW27_11875"
     CDS             1559..2095
                     /locus_tag="VIW27_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740415.1"
                     /translation="MQPIAVCLAAALLAASPVPAQETTRFVETESHSVFTGLFKAVWA
                     RLRSLSPQSRHNASAKVHYTAGIRGAEATDTLLKPYWKDDLTQDAEFQAELESFGEAQ
                     TKMDQGQLEAAVRSFDDFLQQYGDSALRPNALFGKSISLAGIGQTADSLAAMRQFVDE
                     NPSHPLVDDARQVIDNLE"
     gene            2200..2865
                     /gene="tsaB"
                     /locus_tag="VIW27_11880"
     CDS             2200..2865
                     /gene="tsaB"
                     /locus_tag="VIW27_11880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY5740416.1"
                     /translation="MNILAIDTATEACSAAVLRADGALFDVFEIAPRRHNSLLPAMME
                     QVLDAAGIARTELTHCAFTNGPGAFTGIRIAAAQAQGIGLGLGIPLLPVSTLAVLAQT
                     AIDRHGCRRPLAALDARMNEVYWAGYERGADGLACLRGRERLDRVEAIEIDAATDFGA
                     GHGWIEALCGRVEFEVMPGLLPAAKSMLGLAAAAARAGGGVDAAAIGINYLRNRVAEK
                     SAP"
     gene            2862..3899
                     /locus_tag="VIW27_11885"
     CDS             2862..3899
                     /locus_tag="VIW27_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEY5740417.1"
                     /translation="MSDESLPYPYGAPCLAGRIKSCNADFRVDEELGFEPSGEGEHWL
                     LQVEKDGLTTPQLVSRLAADFGLHPRQIAYSGLKDKNALTTQWLSLHLPGKPLPEARR
                     AADGYRVLRAERHRAKLRRGTHRANRFQVRVRGVDGLPEATLQQLAEIASHGMANYFG
                     AQRFGNRGDNVEQALARLGRTRLKRQQQSLLISALRSHLFNQLLARRIREDRWRRPLD
                     GDVFMLRGSRSIFSAPPDDEIRRRFDALDIAATGSLYGAGDSRLGGEALRLERQVFDA
                     HRQICDCLERQRASLQLRPLRVAVEQFDYRLDSRAGLLEVQATLPAGSYLTSLLGHFV
                     SAQEAPQDDLR"
     gene            complement(3881..4516)
                     /locus_tag="VIW27_11890"
     CDS             complement(3881..4516)
                     /locus_tag="VIW27_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5740418.1"
                     /translation="MSEAKGPSEFVDAAYHLEDEASLVEFYRQWARDYDRQMLDELGY
                     VSPVRIGGLLERFLPARDAAILDVGCGTGLTCVPLAAAGYRHLDGIDLSPEMIRVAAE
                     RGIYRELLVGDLNQPLDRDDASYDAVVSTGTFTHGHVGPGPLDELLRILRPGGILACS
                     VHQDLWDSMGFRARFEALAEQGAIECLALEHDSYYREQPPEGWFCVYRKSS"
     gene            complement(4523..5005)
                     /locus_tag="VIW27_11895"
     CDS             complement(4523..5005)
                     /locus_tag="VIW27_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5740419.1"
                     /translation="MTIQIGDRIPAVDVQYMGTDGVETVNTSELCAGKKVVLFALPGA
                     FTPTCSASHLPGYVVKADALKAKGVDLIACLSVNDAFVMDAWGRQNNADDLVMMIADG
                     SAHFSKAVGLDLDLDARGMGIRSQRYSLLINDGVVEQIHLEGPGEFKVSDAETMLANL
                     "
     gene            5088..>5433
                     /locus_tag="VIW27_11900"
     CDS             5088..>5433
                     /locus_tag="VIW27_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5740420.1"
                     /translation="MTRTNLLQARRHWIFDMDGTLTVSAHDFEHMRSELGLAPSTPIL
                     EALNAMPEAEAAPLWDQLNELEFYYAGKASVMEGAAELLQRLHDDGRRLAILTRNTMP
                     VVRQTLDACRLAH"
BASE COUNT         1048 a         1744 c         1713 g          928 t
ORIGIN      
        1 ggtcgaactc gagcccaccg acgaaaatcg cgcgatcctc aacgtggcct tcgcggagcg
       61 caagaaatcc ctcgaattcg aaaacaatgc gccacagctg cgtctctcgg acctcaacct
      121 gcagcgcgtg ttttcggcgg cctacaagaa ataccaggac aggccgatcg gcagcgtcaa
      181 gctggaaaac gtcggtggca ccgagtttgg caacctgaag ctgtcgtttc agatcaagga
      241 attcatggat tttccgagcg ccgtcgagat tccgagaatc gccgccaacg aaaccctgga
      301 aatcccgatc aaggcgacct tcaacaatcg tatcctggaa gtcgacgagg ataccggcgt
      361 ccaggtcgag gtccgcctga gttacctgcg cgacggccag aatgacgaca tctcgctgac
      421 ccagccgatg acgatttacg gcaagaacgc gatcgtctgg aaagatccgc acatgatcgg
      481 ctcgttcgtc accccgaagg acgatacgct gcgcaactac gtgcgccagg tcgtcaacag
      541 ctttcagccc gacccgggcc cgctcaacga caagctggtc gccgcgatgt cctatttcag
      601 cagcctgacg gcttccggca ccagctatat cgtcgatccc aacacaccgt ttaccgagtt
      661 gcgcgacgac cagatcgatt acgtacagtt tccgcgcgaa acgctgcgcc tgaagagcgg
      721 cgactgcgac gacctgtcgg tgctgatcag cgccgggctc gaaaacctcg gcatcaggac
      781 ggccttcgtc gaggtgccgg gacacctgtt cctgatgttc gataccgggc tcgaagccgg
      841 gctcgcggat ttgatcagcc aggatcgcaa cctgctggtg gttcgcgacg atacggtgtg
      901 gattccactc gaggcgacga tgatcaatac cgatttcaac gaggcctggg cggagggtgc
      961 gcgcaagtac caggcggcgc tggccgccga acagctcggc atcatcgacc tcaggcaggc
     1021 ctggcagtcc tacaagccgg ttaccctgcg ccaggccaac tacaatatcg atctgccgca
     1081 gccgcagcgc gccgcgagcc tggtcaacca ggctcgcaag gtgttgctgg cgaagagtat
     1141 cgaccgcctg atcctgcctt accagacgat ggtcgccaac aatcccaagg atctcgcggc
     1201 acgcctgcag atcgcgatcc tgtacacgcg ctacggtctc tatgcggacg ccgagctcgc
     1261 attcgaggcg ctcagcgaac tggctccgca gagcagcgcg gtcaagacca accagggcaa
     1321 cctgtatttt ctgcagggaa attatgatcg cgcgatcgaa aactaccgcc gcgcggccga
     1381 tctcgacacc gaggacggcg gcatactgat caacctgtcg atggcccact acaaggccgg
     1441 ggatttgacc aaagcgagaa ccagttatca aaatgctcta gaattgaatg ctagcttgca
     1501 gcaggaatac gacgcataca gcaagctttt aagtcaatag ggatgaattg atatgaagat
     1561 gcaaccgatt gccgtctgcc tggccgccgc cttgctggcc gcctcgccag tgccggcgca
     1621 ggaaaccact cgcttcgtcg agaccgagtc gcacagcgtt ttcaccggcc tgttcaaggc
     1681 cgtctgggcg cggcttcggt cgttgtcgcc gcagagtcgg cataatgcca gtgccaaggt
     1741 ccactacacc gcgggtattc gcggcgccga ggctaccgat acgctgttga agccctactg
     1801 gaaagacgat ttgacgcagg atgcggagtt ccaggccgag ctcgagagct ttggcgaggc
     1861 gcagacaaaa atggaccagg ggcagctcga agcggcggtc cgctcgttcg acgattttct
     1921 gcagcagtac ggcgatagcg cattacggcc caacgcactg ttcggcaaga gcatcagtct
     1981 cgccggaatc gggcaaactg ccgattcgct ggccgccatg cgccagttcg tcgacgaaaa
     2041 tccgtcgcat ccgctggtcg acgatgcgcg ccaggtaatc gataacctcg aatagcgcct
     2101 ggcccgccgc cccgcggcgc tccgccgata tccgccaggc gtcgaaccgc gacacaaata
     2161 gcggtttatc ggcgcccgcc cgcccgatat catagcggcg tgaatattct tgccatcgat
     2221 accgccaccg aggcctgttc tgcggccgtg ctgcgtgccg acggcgcgct tttcgacgtc
     2281 ttcgaaatcg cgccgcgccg ccacaactcg ctgttaccgg cgatgatgga acaggtgctc
     2341 gacgccgccg gcatcgccag gaccgaactc acccattgcg ccttcaccaa cggtccggga
     2401 gcgttcaccg ggattcgcat cgccgccgcg caggcgcagg gtatcggcct cggcctcggc
     2461 attccgctgt tgccggtatc gacgctcgcg gtgctcgcgc agacggcaat cgaccggcat
     2521 ggctgtcggc ggccgctggc ggcgctcgat gcgcgcatga acgaagtcta ctgggccggc
     2581 tacgagcgcg gcgcggacgg cctggcatgc ctgcgggggc gcgagagact ggaccgtgtc
     2641 gaggcgatcg aaatcgatgc cgcgaccgat ttcggcgccg gccacggctg gatcgaggca
     2701 ctttgcgggc gggtcgaatt cgaggtgatg ccggggctgt tgccggcggc gaagtccatg
     2761 ctcggcctgg cggcagccgc ggcgcgcgct ggcggcggtg tcgacgcggc cgccatcggc
     2821 atcaattatc tgcgtaaccg ggtcgccgag aaatccgcac cttgagcgac gaatcgctgc
     2881 cttatccgta tggtgctccc tgcctggccg gccgcatcaa gtcgtgcaac gccgatttcc
     2941 gggtcgacga ggaactcggt ttcgaaccct cgggagaagg cgagcactgg ctgctgcagg
     3001 tcgaaaaaga cgggctgacc acgccgcaac tggtgtcccg gctggcggct gacttcgggc
     3061 tgcacccgcg gcagatcgcc tacagcgggc tgaaggacaa aaacgcactg accacgcagt
     3121 ggctgagcct gcatttgccg ggcaagccgc tacccgaagc gcggcgtgcc gccgatggct
     3181 accgggtgct gcgcgccgag cgacatcgcg ccaagctgcg ccgcggcacc caccgggcca
     3241 accgtttcca ggtgcgggta cgcggcgtcg acggcctgcc cgaggcgacc ctgcagcaac
     3301 tggcggagat cgccagccac ggcatggcga attatttcgg tgcccaacgt ttcggcaatc
     3361 gcggcgacaa cgtcgaacag gcgctcgccc ggctcggccg gacgcggctc aagcgccagc
     3421 agcaaagcct gctgatatcg gcgctgcgca gccacctgtt caaccagttg ctggcgcggc
     3481 gaattcgcga ggatcgatgg cggcggccgc tcgatggcga tgtcttcatg ctgcgcggca
     3541 gccgctccat attcagtgcg ccgccggacg atgagatcag gcggcgattc gacgcgctgg
     3601 acattgccgc caccggcagc ctgtacggtg ccggcgacag tcgcctgggc ggcgaagccc
     3661 tgcgcctcga gcgccaggta ttcgacgcgc atcggcaaat ctgcgattgt ctcgaacggc
     3721 agcgggcgag cctgcagttg cggccgctgc gggtcgcggt cgagcaattc gattaccggc
     3781 tggacagccg cgccgggctg ctcgaggtgc aggcgacctt acccgcggga agttacctga
     3841 ccagcctgct cggccatttc gtcagcgcgc aggaggctcc tcaggacgac ttgcggtaga
     3901 cgcaaaacca gccctcgggc ggctgttcgc ggtagtagct gtcgtgctcg agagccaggc
     3961 attcgatcgc accctgctcg gccagcgcct cgaaacgggc gcgaaatccc atcgaatccc
     4021 acaggtcctg gtgcacgctg caggcgagga tgccgccagg cctgagaatt cgcagcagtt
     4081 catcgagcgg tcccggaccg acgtggccgt gggtgaaagt accggtcgac acgacggcgt
     4141 cgtaactggc gtcgtcgcga tccagcggct ggttcaggtc gccgaccagc aattcgcgat
     4201 aaatgccgcg ctcggcggcg acgcgaatca tttccggcga caggtcgata ccgtcgaggt
     4261 gccggtaacc ggccgcggcg agcggcacgc aggtcaggcc ggtgccgcag ccgacatcga
     4321 ggattgccgc atccctggcg ggcaggaagc gctccagcaa gccgccgatg cggaccggcg
     4381 acacgtagcc gagttcgtcg agcatttgcc gatcatagtc gcgcgcccac tggcgataaa
     4441 attcgaccag gctggcttcg tcctcgaggt gataggcggc gtcgacgaac tcgctgggtc
     4501 ccttcgcttc gctcacggcc ggtcagaggt tcgcgagcat ggtttcggcg tcgctgacct
     4561 tgaattcgcc ggggccttcg aggtggattt gttcgaccac gccgtcattg atcagcagcg
     4621 agtagcgctg cgagcggatg cccatgccgc gcgcgtcgag atcgagatcg agaccgaccg
     4681 ccttgctgaa gtgcgcgctg ccgtcggcga tcatcatcac caggtcgtcg gcattgttct
     4741 gccggcccca ggcgtccatg acgaaggcat cgttgaccga caggcaggcg atcaaatcga
     4801 cgcccttggc tttcagcgcg tcggctttca cgacgtagcc gggcaggtgg gaagccgagc
     4861 aggtcggcgt gaaggcaccc ggcagagcga acagcacgac cttcttgccg gcgcaaagct
     4921 cgctggtatt taccgtttcg acaccgtcgg tgcccatgta ctggacgtcg accgcaggaa
     4981 tcctgtcgcc aatctgaatt gtcatcgttt gcctctggtt ggttcaatta cgaaaccgtg
     5041 atagtacaat gaaaatcgac aaacccaaat gccggaccga ttcgccgatg acccgaacca
     5101 acctgctgca ggcccgccgt cactggatct tcgacatgga cggcacactg accgtcagcg
     5161 cgcacgattt cgagcatatg cgcagcgaac tcggtctggc gccgtcgaca ccgatactcg
     5221 aagccctcaa cgcgatgccc gaggccgagg cggcgccgtt atgggaccag ctcaacgaac
     5281 tcgagttcta ctatgccggc aaggcgtcgg taatggaggg cgcggccgag ctgctgcagc
     5341 ggctgcacga cgacggccgc cggctggcga tcctgacccg caacaccatg ccggtggtcc
     5401 ggcagacgct ggatgcctgc cggctggcgc att
//
LOCUS       DATCZV010000525         6343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_95301, whole genome shotgun sequence.
ACCESSION   DATCZV010000525 DATCZV010000000
VERSION     DATCZV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95301"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..613)
                     /gene="mreD"
                     /locus_tag="VIW27_11905"
     CDS             complement(134..613)
                     /gene="mreD"
                     /locus_tag="VIW27_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY5740421.1"
                     /translation="MPPLKYMVIYLSLLVALILMILPLPDGVQIYRPNWVALALIYWS
                     MALPKRVGLWFAFFTGIILDTSQGTLLGQHTLALVVIIFLNMNFYQRIRVLALAQQAA
                     YVFVLLLINQLLVAWVEGILGRPTPVLAYFGAPLVGMLIWPWVFVVLRDIRRKAALS"
     gene            complement(615..1433)
                     /gene="mreC"
                     /locus_tag="VIW27_11910"
     CDS             complement(615..1433)
                     /gene="mreC"
                     /locus_tag="VIW27_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5740422.1"
                     /translation="MNILLQARGASLKAFFLIALSITMMVVDHRFHQLEVVRTNISLA
                     LTPLRYLVSLPATGGSWLGDWLTSHSQLMEENEALRARSRILNARLQKLEILAEENAR
                     LRSLLGSARKISDDVIVAELLSVAQSPYRQLIEINKGSSDGVEIGHAVIDDSGVMGQV
                     VHVNPQSATIILISDPEHAIPVQFVRSGTRSVAFGRGSTTQLELRYLPATADIQIDDE
                     LVTSGLGGRFPADYPVAKVTAINEDRVRGFVTVFAEPRARLDSSREVLVIRPRQ"
     gene            complement(1456..2502)
                     /locus_tag="VIW27_11915"
     CDS             complement(1456..2502)
                     /locus_tag="VIW27_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5740423.1"
                     /translation="MFGAIRGLLSNDLSIDLGTANTLIYSRGQGIVLNEPSVVAIRQD
                     RGPGGPKSIEAVGTDAKKMLGRTPENITAKRPMKDGVIADFTYTEKMLQHFIRKVHEN
                     QFMRPSPRVLICVPCGATQVERRAIRESALGAGARKVYLIEEPMAAAIGAGMPVDEAQ
                     GSMVLDIGGGTSEVAVMSLNGIVYSASVRIGGDRFDDAIINYVRRNYGILIGEATAER
                     IKVEIAAAYPGKDLLEMEVKGRNLSEGVPRSFTLNSNEILEALQDPLYGIISAVKNAL
                     EQTPPELGSDIAERGIVLTGGGALLRDLDRLIMEETGLPVIIAEDPLTCVARGGGRVL
                     EMIDEHGLDFLAVE"
     gene            2716..3003
                     /gene="gatC"
                     /locus_tag="VIW27_11920"
     CDS             2716..3003
                     /gene="gatC"
                     /locus_tag="VIW27_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY5740424.1"
                     /translation="MSLKSDDVRAIAHLARLHIDDDAVDRFAADLSSILDLVEQMNRV
                     DSSGVEPLANPLDATQRLRDDKVTENDQRDKFQRIAPDVADGFYRVPKVIE"
     gene            3003..4454
                     /gene="gatA"
                     /locus_tag="VIW27_11925"
     CDS             3003..4454
                     /gene="gatA"
                     /locus_tag="VIW27_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY5740425.1"
                     /translation="MTTGISKIRQGLAAGEFSSVEITSDLLARIEQHNPALNCYISVT
                     GDIALEQARAADAMLAAGNGGALTGVPLAHKDLWCTDGVRTSCGSRMLDNFIAPYDAT
                     VIERFKQAGAVMLGKTNMDEFAMGSSNENSWYGSVANPWRTDCVPGGSSGGSAAAVAA
                     GLAPGAPGTDTGGSIRQPAAFCGVTGIKPTYGRVSRYGMIAYGSSLDQGGPFARSAED
                     CALLLQAMSGFDPRDSTSSEQPVPDFGAALGRGIDGLKIGLPKEFFADGLDARVAAVI
                     DSAVDELRKLGAEISEVSLPNTGLSIPTYYVIAMAECSSNLSRFDGVRFGYRCESPVD
                     LEDLYKRSRGEGFGAEVKRRIMIGTYALSAGYYDAYYLKAQQLRRLIKEDFQRAFEQV
                     DLILGPTTPGVAFAIGDKTDDPVSMYLQDIYTIALNLAGLPGMSLPVGFVDGLPVGMQ
                     LIGNYFDEEGLLNAAHRYQLVSDWHLRVPEAYR"
     gene            4451..5890
                     /gene="gatB"
                     /locus_tag="VIW27_11930"
     CDS             4451..5890
                     /gene="gatB"
                     /locus_tag="VIW27_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY5740426.1"
                     /translation="MSDWETVIGLEIHAQLATRSKIFSGASTAFGAAPNTQACAVDLG
                     LPGVLPVLNREAVAMAIRFGVAIDAQIDRHSVFARKNYFYPDLPKGYQISQYELPIVG
                     KGHVFISLEDGARKEIGITRAHLEEDAGKSIHDQFAEQTGIDLNRAGTPLLEIVSEPD
                     MRSAKEAVAYARKIHSLVRYLEICDGNMQEGSFRCDANVSLRRRGDSELGTRTELKNI
                     NSFRFLERAINFEIERQADLLEDGGKVVQETRLYDAERDETRSMRSKEEANDYRYFPD
                     PDLLPVAIRDADIARIRGALPELPEQKRQRYIDEFGLSEYDAEVLTATRETAAYFEAA
                     LAASPAQCKQLANWVLGDLAAALNRNGIEIAQSPIAPPMLAQLVARIADDTISGKIAK
                     EVFEAMWTGEGGADEIIAARGLQQITDSGAIETVIDAVIAANPEQVEQFRAGKDKVLG
                     FFVGQVMKQTQGKANPGQVNQILRERLKS"
     gene            5892..6224
                     /locus_tag="VIW27_11935"
     CDS             5892..6224
                     /locus_tag="VIW27_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740427.1"
                     /translation="MLTIKRTCSNRIITRALNADGEPLVALLLPGAEDCEVCRNARQL
                     QARLEAEAEAIIVYNQHADAIALVDGLEVAPAQIFIEIRQDTRGVLGLQAYRKQGHAT
                     EPMELFHE"
     gene            6265..>6343
                     /locus_tag="VIW27_11940"
     CDS             6265..>6343
                     /locus_tag="VIW27_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5740428.1"
                     /translation="MSKVLLITGASRGIGAETARLAAARG"
BASE COUNT         1224 a         1997 c         2000 g         1122 t
ORIGIN      
        1 tcgggatagt aagtgctctc gcgaatgaac tcggtgtcga tcgcgcagca accggcttcg
       61 gcgagcgcgt cgcagaaacc ggcgagcgcc gcgtcggaat cgatgtagtc ataatcggcg
      121 ggtgcgttca aggtcagctc agtgcggcct tgcgccgaat atcgcgcagc acgacgaaca
      181 cccagggcca gatcagcata ccgaccaggg gtgcgccgaa gtaggcgagc accggcgtcg
      241 gccgcccgag tatgccctcg acccaggcca ccagcagctg gttgatcaac agcagcacaa
      301 agacgtaggc ggcttgctgg gcgagcgcca gcactcgaat gcgctggtag aagttcatgt
      361 tgagaaagat aatcaccacc agcgccaacg tatgctgacc gagcagcgtt ccctgcgagg
      421 tgtcgagtat gatgccggta aagaacgcga accacagccc gacgcgcttg ggcagcgcca
      481 tgctccagta gatcagcgcc agcgccaccc agttggggcg atagatctgc acgccgtccg
      541 gtagcggcag aatcatcagg atcagcgcca ccagcaggct caggtagatg accatgtact
      601 tgagtggcgg catgttactg ccgcggccga atgacgagta cctcgcggct ggaatcgagg
      661 cgcgcccggg gctcggcgaa cacggtgacg aagccgcgca cccggtcctc gttgatcgcc
      721 gtgaccttcg ctaccgggta gtcggcagga aaacggccac cgagcccgga agtcaccagt
      781 tcgtcgtcga tctggatgtc ggcggtggcc ggcaggtagc gtaactcgag ctgagtagtc
      841 gagccgcggc caaacgccac cgagcgcgtg ccgctgcgca cgaactgcac cggaatcgcg
      901 tgctcgggat cggaaatcag gatgatggtc gcggactgcg gattgacgtg cacgacctgg
      961 cccatgaccc cggaatcgtc gatcaccgcg tgaccgatct cgacaccgtc actgctgccc
     1021 ttgttgatct cgatcagctg acgatagggg ctttgcgcga ccgacagcaa ttcggcgacg
     1081 atgacgtcgt cggaaatctt gcgcgccgag ccgagcaggc tgcgcaaacg cgcgttttcc
     1141 tcggcgagga tttcgagctt ttgcaggcgc gcattgagga ttcggctcct ggcgcgcagc
     1201 gcctcgttct cctccatcag ctggctgtgc gaagtcagcc agtcacccag ccagctgccg
     1261 ccggtagccg gcagactgac caggtaacgt aacggggtga gcgccagcga aatattggtg
     1321 cgcacgactt cgagctggtg aaaacgatgg tcgacgacca tcatcgtgat gctgagcgca
     1381 atcaggaaga acgccttgag cgatgcgcca cgtgcctgca gtaaaatatt cattcgggcg
     1441 gtggcgacgg gaagactact ctaccgccag gaagtccagc ccatgttcat cgatcatttc
     1501 gagcacccgg ccgccgccac gggcgacgca ggtcagcgga tcctctgcga taatcacggg
     1561 cagcccggtt tcttccatga tcaggcggtc gagatcccgc agtagcgcac cgccgccggt
     1621 gagcacgatg ccgcgctcgg cgatatccga gccgagttcc gggggtgtct gttcgagcgc
     1681 gttcttgacc gctgaaatga ttccgtagag cgggtcctgc agggcttcca gaatttcgtt
     1741 gctgttgagg gtgaagctgc gcggcacgcc ttccgacagg ttgcggccct tgacttccat
     1801 ttccagcaag tccttacccg gataggccgc ggcgatttcg accttgatcc gttcagcagt
     1861 tgcttcgccg atcaggatac cgtagttgcg gcgcacgtag ttgatgatcg catcgtcgaa
     1921 gcggtcgccg ccaatgcgta ccgaggccga gtagacgatg ccgttcagcg acatgaccgc
     1981 gacctccgag gtgccgccgc cgatgtcgag caccatcgag ccctgtgcct cgtcgaccgg
     2041 catgccggca ccgatcgcgg ccgccatcgg ctcctcgatc aggtacacct tgcgcgcgcc
     2101 ggcgccgagc gccgattcgc ggatcgcgcg gcgttcgacc tgcgtggcgc cgcagggcac
     2161 gcagatcagc acgcgcgggc tgggccgcat gaactggttt tcgtgcacct tgcgaataaa
     2221 gtgctgcagc atcttttcgg tgtaggtgaa gtcggcgatc acgccgtcct tcatcggccg
     2281 cttggcggtg atattttcgg gtgttctgcc gagcatcttc ttggcgtcgg taccgaccgc
     2341 ctcgatcgat ttggggccac caggcccgcg gtcctggcga atcgcaacca ccgaaggttc
     2401 gttgagtacg attccctgcc cgcgggaata aataagcgta ttggccgtac ccagatcgat
     2461 cgagaggtcg ttggataaca ggccgcggat agctccaaac atgctctgtg ttttaacccg
     2521 ttgtgtatat tttggcgtgt gtgcgcacga aatttcctgc tgctcgctta gtatagcgca
     2581 gaatcgagcg tactatacta cgagtcgcga ttttaacaag gtgaaatacc ttacgaatca
     2641 gtcttttgct tatacaactg gtgaaattgt ggtaagtttg ctggcttttc catcgaagct
     2701 tggttgtttg ccgaaatgag cctcaaatcc gacgatgttc gcgccattgc gcacctcgcg
     2761 cgactgcata tcgatgacga tgcggtcgat cgcttcgccg ccgacctgtc gagcattctc
     2821 gatctagtcg aacagatgaa ccgggtcgat agcagcggcg tcgagccgct cgccaatccg
     2881 ctcgatgcga cccagcggct gcgcgacgat aaagtcaccg aaaacgacca gcgcgacaag
     2941 ttccagcgca tcgcgccgga cgtcgccgac ggcttctacc gcgtgcccaa ggtgatcgag
     3001 taatgacgac gggaatcagt aagattcgcc agggtctcgc cgccggcgaa ttctccagcg
     3061 tcgaaatcac cagcgatctg ctggcgcgta tcgaacagca caacccggcg ctgaactgct
     3121 acatcagtgt caccggcgat atcgccctcg agcaggcgcg agccgccgac gcgatgctgg
     3181 ccgccggcaa cggcggggcg ctgaccggcg tgccgctggc gcacaaggac ctgtggtgta
     3241 ccgacggcgt gcgcacttcc tgcggttcgc ggatgctgga taatttcatc gccccctacg
     3301 acgccaccgt aatcgagcgc ttcaagcagg ccggcgcggt gatgctcggc aagaccaaca
     3361 tggacgagtt cgcgatgggt tcgtcgaacg aaaactcgtg gtacggcagc gtcgccaacc
     3421 cgtggcgaac cgattgcgtg ccaggcggtt cgtccggcgg ctcggcggcc gcggtcgccg
     3481 ccggcctcgc gcccggcgcc cccggcaccg ataccggcgg ctcgattcgc cagccggccg
     3541 cattctgcgg cgtgaccggc atcaagccga cctacgggcg cgtgtcgcgc tacggcatga
     3601 tcgcctacgg ctcgagcctc gaccagggcg gcccgttcgc gcgcagcgcc gaggattgcg
     3661 cgctgctgct gcaggcgatg tccggtttcg atccgcgcga ctcgacttct tccgagcagc
     3721 cggtgccgga cttcggcgca gcactcgggc gcggtatcga cggtctgaag atcggcttgc
     3781 caaaggaatt tttcgccgac ggcctcgacg ctcgcgtcgc cgcggtcatc gacagcgccg
     3841 tcgacgaatt gcgaaaactc ggtgccgaga tcagcgaggt ctcgctgccc aacaccgggc
     3901 tatcgatacc gacctactac gtgatcgcga tggccgaatg ctcgtccaac ctgtcgcgct
     3961 tcgacggcgt gcgcttcggt taccgctgtg aaagcccggt cgatctcgag gacctctaca
     4021 agcgctcgcg cggcgagggt ttcggtgccg aggtcaagcg ccgcatcatg atcggcacct
     4081 atgccttgtc ggcgggttac tacgatgcct actacctgaa ggcgcagcaa ctgcggcgcc
     4141 tgatcaagga ggacttccag cgcgccttcg aacaggtcga cctgattctc ggcccaacca
     4201 cgcccggggt ggcattcgcg atcggcgaca agaccgacga cccggtcagc atgtacctgc
     4261 aggatatcta caccatcgcg ctgaacctcg ctggcctgcc gggcatgtcg ttgccggtcg
     4321 gcttcgtcga cggcctgccg gtcggcatgc agctgatcgg caattacttc gacgaagagg
     4381 gcctgctgaa cgcggcacac cgctaccagc tggtcagcga ctggcatctg cgggtaccgg
     4441 aggcgtatcg atgagcgact gggaaaccgt catcgggctc gagatccatg cgcagctcgc
     4501 cacccgcagc aagattttct ccggcgcctc gaccgcgttc ggcgccgcgc ccaacaccca
     4561 ggcctgcgcg gtcgatctcg gcctgcccgg cgtgctgccg gtactcaatc gcgaggcggt
     4621 ggcgatggcg attcgcttcg gtgtcgcaat cgacgcgcag atcgaccgcc actcggtgtt
     4681 cgcgcgtaag aattactttt atcccgatct gcccaagggt taccagatct cgcagtacga
     4741 gctgccgatc gtcggcaaag gacacgtgtt catcagcctc gaagacggcg cacgcaaaga
     4801 gatcggcatc acccgcgcgc atctcgagga agacgccggc aagtcgatac acgaccagtt
     4861 cgccgagcag accggtatcg acctgaaccg cgccggcacc ccgctgctgg aaatcgtttc
     4921 cgagcccgac atgcgctcgg cgaaagaggc ggtcgcctac gcgcgcaaga ttcattcgct
     4981 ggtgcgctac ctggagatct gcgacggcaa catgcaggaa ggctcgtttc gctgtgacgc
     5041 caacgtctcg ttgcggcggc gcggcgacag cgaactcggc acgcgcaccg agctcaagaa
     5101 catcaactcg tttcgcttcc tcgagcgcgc catcaatttc gagatcgagc gccaggccga
     5161 cctgctcgaa gatggcggca aggtggtgca ggagacccga ctctacgatg ccgagcgcga
     5221 cgagacacgc tcgatgcgca gcaaggaaga agccaacgat taccgttatt ttcccgatcc
     5281 cgacctgttg ccggtggcga tccgcgatgc cgatatcgcg cgcattcgcg gcgcgctgcc
     5341 ggagttaccg gagcaaaagc ggcagcgata tatcgacgag ttcgggctct ccgagtacga
     5401 cgccgaggta ctgaccgcaa cccgcgaaac cgcggcttac ttcgaggccg cactcgccgc
     5461 gtcaccggcg cagtgcaagc agctcgccaa ctgggtgctc ggcgatctcg ccgccgcgct
     5521 caacaggaac ggtatcgaga tcgcgcaatc gccaatcgcg ccgccgatgc tggcgcaact
     5581 ggtcgcgcgc atcgccgacg acacgatttc cggcaagatc gccaaagaag tcttcgaggc
     5641 gatgtggacc ggcgagggcg gcgccgacga aatcatcgcc gcccgcgggc tgcagcagat
     5701 taccgacagc ggtgccatcg agacggtgat cgacgcggtg atcgccgcca atcccgagca
     5761 ggtggagcaa ttccgtgccg gcaaggacaa ggtgctcggt ttcttcgtcg gccaggtcat
     5821 gaagcagacg cagggcaagg cgaatcccgg acaggtcaac cagattctgc gcgagcggct
     5881 gaaatcctga catgctgacc atcaaacgca cctgcagtaa ccggatcatt acccgtgcgc
     5941 tgaacgccga cggcgaaccg ctggtggcgc tgctgctgcc gggcgccgag gattgcgagg
     6001 tatgccgcaa cgcgcggcaa ttacaggcgc ggctggaggc cgaagcggaa gcgattatcg
     6061 tttacaatca gcatgccgac gcgatcgcgc tggttgacgg gctagaggtc gctccggcac
     6121 agatattcat cgagattcgg caggatacca ggggcgtgct gggcctgcag gcttaccgca
     6181 agcagggcca cgcgaccgag ccgatggaat tgtttcacga gtaaacgccg cggggggctc
     6241 gcggccgaaa ggataaccca ggcgatgtcg aaagtgttat tgatcaccgg cgcgagccgc
     6301 ggcatcggcg ccgaaaccgc gcgcctggcc gccgcgcggg gct
//
LOCUS       DATCZV010000526         4478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_784556, whole genome shotgun sequence.
ACCESSION   DATCZV010000526 DATCZV010000000
VERSION     DATCZV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784556"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4478
                     /locus_tag="VIW27_11945"
     CDS             <1..>4478
                     /locus_tag="VIW27_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY5740429.1"
                     /translation="VVLIVGERGVLKRQPIELIKNFLYYIAQPECDGPKAQAIKTAYR
                     LEQFLPSESARSEVLSNIAGPNQALMKTVGEAMKADIEAVKSTLEVYVNGDLSRVSAL
                     KNLPKELHVISDTLAMIGLGPERQLVEAQIELVKDMLQGEKAADEEQLLAMAAELIQV
                     EQALDRMQKRQPAASSGEAAADTNVSRDYELDNVLTAVVTAGLDNIQKTKNAILEFIK
                     DSSHTENIDYSVAMMEEARGAMELLNQPRAVAVVDGLLTYLKGYDIVEFMESSRLDAL
                     SQVVVSLEYYLEALGEHRGDANTILDLADTQLKELLANVRLRQEGEYTQVLELIEDTV
                     EMPVVATPPAESEAPAAADAKTPAAAEQVPADTDRVPNLAAATITMAPVFDDTQMKIE
                     EIVAKQDWSSAPSAGSQPSVRQKSQAEPPPPEDMLKVTPVKDELEVLKPGSDPEILSI
                     YLEEAQEEAMNIARLQKDWMLHPEDENAYKNIRRAFHTIKGSGRLVGAMKIGEFAWEF
                     EQLLNRVIDKTVAPDVKVIEAVGKAAWALPELVKELKTGEKPKARIAYLRGLARALAE
                     FKAEQLLIEHTQKMAIVQMSDERGGGEKVPAADVLLGGETMQVQPAQLKAGPSAPQSA
                     SQDDGDFASTQLMDAVDAPDAAGAAESAAAGGDEFESTQLMDAVDAPDAAGAAESAAA
                     GGDEFENTQLMDAAGPADAGADLEDEVVFSEAAQSFLDDPSQFTQAMDYARVDEDDMQ
                     DDVDLPDAIESESLQLAEQDPQAAGSGDAVFGSPQSISAAPAAASGQPDAASAEELEL
                     VALDDEPWSASANAEGEAEEIELQSAVEDEVEEIELQSAAEDEVEEIELQSTAEDEAE
                     EIELQPVAEGEAEEIELQAPAEDDEAVPQAESAAQPVDGPDAAEESPPDPFVTTTTGA
                     LSGREAGASLRASQEPREPSGLSLDPELLTIYLQEVEQHLDIINSALEHAEKIQELIP
                     GEKIYRALHTIHGASRTAEIASIGELADLMEQPLKAAIAQRLALDMEIVSLYRQGQQA
                     IQSMIAELVATHRLPEIPAELKTSFAALAEDFEEYTVELPREGIDDSGNFVDTLTMMN
                     EAAADGQEDELLEIFVDEAKELLEMSDNILHEWAEQGNDDKGSQDFVAVMELQRYLHT
                     LKGGARMAELREIGDLAHELESMFIAVIDGRVEKSDHLIELLKHSFDQLHRQVAEAQQ
                     RQPISDSSNIIGMLRQLRLDENEQQQVEVSDREQATVDREDINIVSENLPEEPLTGPE
                     RQTQSVIRVRADLLDNLVSSAGEASIYRARMEQQVAGLGSYLGELGQTISRLKTQLRN
                     LEAETDAQIHFSHRAETPSSDFDPLEMDRYTLIQELSRSLSESVNDLSSLQTMLGEQV
                     KDSETLLLQQSRVNTDLQDGLIKSRMVRFSGLLSRLRRLVRQSAQELGKKAELAITGE
                     DNEVDSKVLDRMVAPLEHILRNALSHGIETPMERVNKGKSESGRIGIDITREGSEVV"
BASE COUNT         1007 a         1308 c         1480 g          683 t
ORIGIN      
        1 gtggtactga tcgtcggtga gcgcggcgtg ctcaagcgcc agcccatcga gctgatcaag
       61 aattttctct attacatcgc ccagcccgag tgcgacggac ccaaggcgca ggcgatcaag
      121 acggcttacc ggctggaaca gttcctgccg tcggaatcgg cgcgcagcga agtgctgagc
      181 aatatcgccg gccccaacca ggcgctgatg aaaaccgtcg gcgaagccat gaaggccgat
      241 atcgaggcgg tgaaatcgac actcgaggtt tacgtcaacg gcgacctgtc gcgagtcagc
      301 gcgttgaaga acttgccgaa ggagttgcac gtcatttcgg acaccctggc gatgatcggc
      361 ctcggccccg agcgccagct ggtcgaggcg cagatcgagc tcgtcaagga catgctgcag
      421 ggcgaaaagg cggccgacga agaacaactg ctggcgatgg ccgcggaatt gatacaggtc
      481 gagcaggccc tcgaccgaat gcagaaacgg cagcctgccg ccagcagtgg tgaagccgcc
      541 gccgatacca acgtttcgcg agactacgag ctcgacaacg tgctcaccgc ggtggtgacc
      601 gcgggtctcg ataatataca gaaaaccaag aacgcgattc tcgagttcat caaggattcc
      661 agccataccg aaaatatcga ttacagcgtg gcgatgatgg aagaagcccg cggcgcgatg
      721 gaactcctca accagccgcg cgcggtggca gtggtcgacg gcttgctgac ttacctgaaa
      781 ggctacgaca tcgtcgagtt catggaatcc agccgcctcg acgcactgtc gcaggtggtg
      841 gtcagcctcg agtattacct cgaagccctc ggcgaacacc ggggtgacgc caataccatt
      901 ctcgacctcg ccgataccca gctcaaggaa ttgctggcca acgtgcgcct caggcaggaa
      961 ggtgaatata cgcaggtgct ggagctgatc gaggacaccg tcgagatgcc ggtggtggcc
     1021 acgcccccgg ccgaatccga agcgccggcc gcagccgacg cgaaaacccc tgcggcagcg
     1081 gaacaggtac ctgccgacac ggatcgagtg cctaacctgg ccgcggcgac gataaccatg
     1141 gcgccggtct tcgacgacac gcaaatgaag atcgaggaga tcgtcgccaa gcaggactgg
     1201 tcgagtgcgc catcggcggg ctcgcagcca tcggtgcggc aaaagtcgca agccgagccg
     1261 ccgcctcccg aagatatgct caaggtgacg ccggtgaagg atgaactgga ggtgctgaaa
     1321 ccgggcagcg accccgagat tctctcgatc tatctggagg aagcgcagga agaggcgatg
     1381 aacatcgcgc gcctgcaaaa ggactggatg ctgcacccgg aagacgaaaa cgcctacaag
     1441 aacattcgcc gcgcctttca taccatcaag ggtagcggcc gcctggtggg ggcgatgaaa
     1501 atcggcgaat ttgcgtggga atttgagcag ttgctgaacc gcgtgatcga caagaccgtg
     1561 gcccccgacg tcaaggttat cgaagccgtc ggcaaggcgg cctgggcgtt gccggagctg
     1621 gtcaaggagt tgaagaccgg cgagaagccg aaggcgagga tcgcctatct gcgcggtctc
     1681 gcgcgcgcgc tggccgagtt caaagccgaa cagctgctga tcgagcacac gcagaagatg
     1741 gcgatcgtgc aaatgtcgga cgagcggggc ggtggcgaaa aggtgccggc ggccgacgta
     1801 ttgctcggtg gcgagacgat gcaggtgcag cccgcacaac tgaaggccgg gccaagcgcc
     1861 ccgcaatcgg cttcgcagga tgacggcgac ttcgcaagca cccagttgat ggatgcggtg
     1921 gacgcgccgg acgccgcggg tgcggctgaa tcggctgccg ccggcggtga cgaattcgag
     1981 agtacccagt tgatggatgc ggtcgacgcg ccggacgccg cgggtgcggc tgaatcggct
     2041 gccgccggcg gtgacgaatt cgagaatacc cagttgatgg atgcggcggg cccggcggac
     2101 gccggcgcgg atctggaaga tgaagtggtc ttcagcgagg cggcacagtc tttcctcgat
     2161 gatccgtcgc agtttaccca ggcgatggat tacgcacggg tcgacgaaga cgatatgcag
     2221 gatgacgtcg acctgccgga cgcaatcgaa tccgagtcgc tgcaactcgc ggagcaggat
     2281 ccgcaggcgg caggttcggg cgatgccgtt ttcggttcgc cgcaaagcat ctcggcggct
     2341 cccgcggccg cctcgggtca gcccgatgcg gcgagcgcag aggaactgga gctggtggcg
     2401 ctcgatgatg agccgtggag cgcttcggcg aacgccgaag gcgaggccga agagatcgag
     2461 ctgcagtcgg cagtcgagga cgaggtcgaa gagatcgagc tgcagtcggc agccgaggac
     2521 gaggtcgaag agatcgagct gcagtcgaca gccgaggacg aggcggaaga gatcgagctg
     2581 cagccggtgg ccgagggcga ggcggaagag atcgaattac aggcgcccgc cgaggatgac
     2641 gaagctgtgc cgcaagcgga atccgcagcg cagcccgtgg atggcccgga tgctgccgag
     2701 gagagcccgc ccgacccttt cgtgacgacg acaacggggg ccctgtctgg aagagaggca
     2761 ggagccagcc tgcgcgcgag ccaagagccg cgcgaaccaa gcggcctgtc gctggacccc
     2821 gagttactga cgatctacct acaagaggtc gaacagcacc tcgacataat aaattccgcc
     2881 cttgagcacg cggaaaagat tcaggaactg atcccgggcg agaaaatcta ccgggccctg
     2941 cataccattc acggtgcctc gcgtaccgcc gaaatcgcca gtatcggcga actcgccgac
     3001 ctgatggaac agccgttgaa agcggcaatc gcacagcggc tggctctcga catggaaatc
     3061 gtgtccctct accggcaggg tcagcaagcg atccagtcga tgatcgccga actggtcgcc
     3121 acccatcgcc tgcccgagat tcccgcggag ctgaaaacca gtttcgcggc actggccgag
     3181 gatttcgagg aatacacggt cgaactgccg cgggaaggta tcgacgacag tggtaatttc
     3241 gtcgatacgc tgacgatgat gaacgaggct gccgccgacg gtcaggaaga cgaactgctg
     3301 gaaatcttcg tcgacgaggc aaaggaactg ctggaaatga gcgacaacat attgcacgag
     3361 tgggccgagc agggtaacga cgacaagggc agccaggatt tcgtcgcggt gatggaatta
     3421 cagcgctacc tgcacaccct caagggcggg gcgcggatgg cggagctgcg cgagatcggc
     3481 gatctcgcgc atgagctgga gtcgatgttc atcgcggtca tcgatggccg agtcgaaaaa
     3541 agcgatcacc tgatcgaatt gctcaagcac agcttcgacc agctgcaccg gcaggttgcc
     3601 gaggcacagc aacgccagcc gatcagcgat tcatcgaata tcatcggcat gctgcggcag
     3661 ctgcgtctcg acgagaacga gcagcaacag gtggaagtca gcgaccgcga gcaggctacc
     3721 gtcgatcgcg aagacatcaa tatcgtcagc gagaacctgc ccgaggagcc gctcaccggg
     3781 ccggagcggc agacccagag cgtgattcga gtgcgcgccg acctgctcga caacctggtc
     3841 agttccgccg gcgaagccag tatttaccgt gcgcgcatgg aacagcaggt cgccggtctc
     3901 ggctcctacc tcggcgaact cgggcaaacc atctcgcgcc tgaagaccca gctgcgcaat
     3961 ctcgaggcgg agacggatgc gcaaatacat ttcagccacc gcgccgagac gcccagttcg
     4021 gacttcgacc cgctggagat ggatcgttac acgctgatcc aggagttgtc gcgttcgctc
     4081 agcgagtcgg tcaacgatct gtccagcctg cagacgatgc tcggcgaaca ggtcaaggat
     4141 tcggaaaccc tgctgttgca gcagtcgcgg gtcaataccg acttgcagga tggcctgatc
     4201 aagagccgca tggtgcgctt ttcgggcctg ttgtcgcggt tgcgccggct ggtcaggcaa
     4261 agcgcccagg aactcggcaa gaaggccgag ctggcgatca ccggcgaaga caacgaagtc
     4321 gacagcaagg tgctcgaccg catggtcgcg ccgctcgagc acatcctgcg caatgccctg
     4381 tcgcatggca tcgaaacgcc gatggaacgg gtcaacaagg gcaagtcgga aagcggccgg
     4441 atcggcatcg atatcacccg cgaaggctcc gaggtggt
//
LOCUS       DATCZV010000527         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_95326, whole genome shotgun sequence.
ACCESSION   DATCZV010000527 DATCZV010000000
VERSION     DATCZV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95326"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIW27_11950"
     CDS             <1..251
                     /locus_tag="VIW27_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012140987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY5740430.1"
                     /translation="VASGLSLQTYDSLMKGTKFGSVVLPGSAVSSTFYRVITHEVDPK
                     IRMPPHNKESLAKGRGEMLSDGQIKDIELWIDQGAKNN"
     gene            361..1620
                     /locus_tag="VIW27_11955"
     CDS             361..1620
                     /locus_tag="VIW27_11955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5740431.1"
                     /translation="MTQSESVPTLLPTKRRPILKSSKLENVCYDIRGPVLRAAHRLEE
                     EGHRVYKLNIGNPAPWGFEAPEEILRDVIHNIPSSQGYSDSHGVYSARKAVMQYYQGI
                     GIAGVDIDDVYIGNGVSELVGIAMQALVNDGDEVLIPAPDFPLWTAAVNLCRGKPVHY
                     RCDEGAGWQPDLDDIRARITPKTRALVVINPNNPTGAVYERAMLEALVEIAAEFDLLL
                     LADEIYDKIVYDDAAYIPLASLAPDSLLTLTFSGLSKSYRVAGFRSGWLLVSGDKLRA
                     KDFIEGLDILVSMRLCANVPAQHAIQTALGGYQSINDLVAPGGRLHEQRDRSWELLDG
                     IDGVSCVKPRGALYLFPKLDPRKFNIRDDEKFVLDLLINEKVLVVQGTGFNWPEPDHF
                     RVVFLPHIEDLEESIGRIEHFLSGYRQ"
     gene            1650..>2135
                     /locus_tag="VIW27_11960"
     CDS             1650..>2135
                     /locus_tag="VIW27_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY5740432.1"
                     /translation="MARCDGLFIAAPIGYSCLAAMAPASFNQPIGKGTVMIRNSLYSC
                     LLLVLVAGCATSMTSDISIDTEHDPKVAFGAYKTYAWLGTAAILNDPEGRWEPPTFDA
                     DAEITYLINRQLRARGMVEAQVDPDVLVFYGAGIDMESFEIKVDPETDMEQMINVPMG
                     GL"
BASE COUNT          420 a          657 c          654 g          404 t
ORIGIN      
        1 cggtcgccag cggattgagc cttcaaacct acgatagcct gatgaaaggc acgaaattcg
       61 gctcggtcgt gttgccggga agcgcggtgt cgagcacctt ttaccgcgtt attacccacg
      121 aagtggaccc gaagattcgc atgccgccgc ataacaagga gtccctggcc aagggccggg
      181 gtgagatgtt gagcgatggg cagatcaagg acatcgaact gtggatcgac cagggcgcga
      241 aaaataacta gctcaccggc cgcgcagccg ttggtaacgc agttggctaa ttgcggcgga
      301 atgctattat aagttgcatt tccggccgtc gatgccgggc aacgtcactg caggaacgcc
      361 atgacccagt ctgaatccgt gcccacgctg ctgccgacga agcggcgccc gatactcaag
      421 tcgagcaagc tcgaaaacgt ttgctacgat attcgcggcc cggtgctgcg tgccgcccac
      481 cggctcgagg aagagggcca cagggtctac aagctcaaca tcggcaaccc ggcgccctgg
      541 ggtttcgagg cgcccgagga aatcctgcgc gacgtgattc acaacattcc gtcgtcgcag
      601 ggctatagcg attcgcatgg cgtctactcg gcgcgcaagg cagtcatgca gtactaccag
      661 ggtatcggca ttgccggcgt cgatatcgac gacgtctaca tcggcaatgg cgtatcggaa
      721 ctggtcggca tcgcgatgca agcactggtc aacgatggcg acgaagtgct gatcccggcg
      781 ccggactttc cgctgtggac cgcggcggtc aacctgtgcc gcggcaagcc ggtgcactac
      841 cgctgcgacg agggtgccgg ctggcagccc gacctcgacg acattcgcgc gcgcatcacg
      901 ccgaaaaccc gcgcgctggt ggtgatcaac ccgaacaatc cgaccggcgc ggtctacgag
      961 cgggcgatgc tcgaggcact ggtcgagatc gcggccgagt tcgacctgtt gctgctggcc
     1021 gacgaaatct acgacaagat cgtctacgac gacgcggcct atattccgct cgcctcgctg
     1081 gcgccggaca gcctgttgac gctgaccttc agcggcctgt cgaagtccta ccgggtcgcc
     1141 ggttttcgca gcggctggct gctggtcagc ggcgacaagt tgcgcgcaaa ggatttcatc
     1201 gagggcctgg atatcctggt gtcgatgcga ctgtgcgcca atgtgcccgc gcagcacgcg
     1261 atccagaccg cgctcggcgg ctaccagagc atcaacgacc tggtcgctcc cggcggccgc
     1321 ttgcatgagc agcgtgatcg cagctgggag ctgctcgatg gcatcgacgg cgtcagctgc
     1381 gtcaagccgc gcggcgcgct atacctgttt ccgaaactcg acccgcgcaa gttcaatatt
     1441 cgcgacgacg agaagttcgt gctcgacctg ctgatcaacg aaaaggtact ggtcgtgcag
     1501 ggcaccggct tcaactggcc cgagcccgat catttccgcg tcgtgttcct gccgcacatc
     1561 gaggatctcg aggaatccat cggccgtatc gagcatttcc tgtccggcta tcgccagtag
     1621 ggccggccac cggcaacccc gctcttcgct tggcgcgctg cgacgggctt ttcattgccg
     1681 cgcctatcgg ctacagttgc ctcgctgcga tggcgccagc cagctttaac caaccgatcg
     1741 gaaagggaac agttatgatt cgaaattcgc tctattcgtg ccttctgctg gttctcgtcg
     1801 ccggctgcgc gacctcgatg accagcgata tcagcatcga caccgagcat gatccgaagg
     1861 ttgccttcgg cgcctataag acctatgcct ggctcggtac tgctgccatt ttgaacgatc
     1921 cagaaggtcg ctgggagccg cccactttcg atgcggacgc cgaaatcacc tacctgatca
     1981 accgccaact gcgcgcacgg ggcatggtcg aggcgcaggt cgatcccgat gtgctggttt
     2041 tttacggtgc gggaatcgac atggaaagct tcgagatcaa ggtcgatccg gagaccgaca
     2101 tggaacagat gatcaacgtg ccgatgggcg gcctg
//
LOCUS       DATCZV010000528         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_784604, whole genome shotgun sequence.
ACCESSION   DATCZV010000528 DATCZV010000000
VERSION     DATCZV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784604"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VIW27_11965"
     CDS             <1..396
                     /locus_tag="VIW27_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEY5740433.1"
                     /translation="LGSAVNSVGIDSWLAALVEGAIAGIPLFLALLGLVSCGILVTMV
                     ASNTASAAILIPLMLPLSSSLGIDVKSMAMLIAISVSLDFMMPVGTPPSAIAYSTGVV
                     NVRQMIQNGFRVNIGTGLILVMLYYFFYL"
     gene            655..1116
                     /locus_tag="VIW27_11970"
     CDS             655..1116
                     /locus_tag="VIW27_11970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5740434.1"
                     /translation="MSDRTELDFRVGLFDGDLFGELNNSRYLTFAELARWDYSMRVGF
                     VGVMRRNGWGLAVGGVSLRYRRRIPIFRKFRLTTELVCHDGRWFYFLQEFHRQGKICA
                     SALMKVGATSKRGLVPATEVLDALGESANPEMPEWVGAWIDAEGQRPWPEA"
     gene            1148..1894
                     /locus_tag="VIW27_11975"
     CDS             1148..1894
                     /locus_tag="VIW27_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5740435.1"
                     /translation="MKLRILSDLHVEFEDFEPPPVDADVVVLAGDIHVRHHGVDWARE
                     RFPGVPVLYVLGNHEYYGGAMPAHLETMKQQAAGSNVRVLENEALDIGGVTFLGCTLW
                     TDFALYGKAPLHGFAARRLVTDYRIIRVSPSFHRLRPKHTAELHRQSARWLAETARRY
                     DGENTVVITHHAPSMRSLPDDFRGNPVSAAYASNLDRLVEECGAQLWIHGHLHRQSDY
                     RLGDTRVVCNPRGYPNQRNPSFVPDLVIEI"
BASE COUNT          340 a          627 c          673 g          386 t
ORIGIN      
        1 ctcggctcgg cggtcaacag cgtcggcatc gacagctggc tggccgcgct ggtggaaggc
       61 gcgattgccg gtatcccgct gttcctggcg ctgctcgggc tggtgtcctg cggcatcctg
      121 gtgacgatgg tggcgagcaa caccgcgtcg gccgcgatcc tgattccgtt gatgctgccg
      181 ctgtcgagca gcctcggcat cgacgtcaag tcgatggcga tgctgatcgc gatcagcgtg
      241 tcgcttgatt tcatgatgcc ggtgggtacg ccgccgagcg cgatcgccta ttcgaccggt
      301 gtggtcaacg tgcggcagat gatacagaac ggattccgcg tcaatatcgg caccgggctg
      361 atcctggtga tgctgtatta tttcttctac ctgtaggcgg cccgcgggag ccggtgttcg
      421 ctgcccgaac gcgcgcggat gacgcgccgg tgggaaagtt gaatcgccgg caaccccgca
      481 agcccggccc atgccgcggg ctgccgcgct ggattgcgcg attgctgata aatcccgtag
      541 ccggggaaag ggttccccgc gacgggtcag acgctatact ctgcgagcat catgtatccg
      601 tatcttagat tgatggctat tctgctgact gcgcgtcttc gcccggtgct cggcgtgagc
      661 gacaggacag aactcgattt tcgtgtcggc ttgttcgatg gcgatctgtt cggtgagttg
      721 aacaattccc gctacctgac tttcgccgaa ctcgcgcgct gggattactc gatgcgcgtc
      781 ggcttcgtcg gggtcatgcg gcgaaacggc tggggactcg cggtgggtgg cgtgagcctg
      841 cgctaccggc ggcgcatacc gattttccgc aagttcaggc tgactaccga gctggtctgc
      901 cacgatggcc gctggtttta tttcctgcag gaatttcacc gccagggcaa gatctgcgcg
      961 tcggcgctga tgaaagtcgg cgcgacctcg aagcggggcc tggtgccggc caccgaggtg
     1021 ctcgatgcgc tcggcgaaag cgcgaatccg gagatgccgg aatgggttgg cgcgtggatc
     1081 gacgccgagg gccagcgtcc ctggccggag gcctgagcag gcggcggccg acggacgagc
     1141 ggaagcgatg aagctgcgca tcctgagcga cttgcacgtc gagttcgagg acttcgagcc
     1201 gccgccggtc gacgccgacg tggtggtgct ggccggagat atccatgttc gccatcacgg
     1261 cgtcgactgg gcccgggaaa ggtttcccgg cgtgcccgtc ctctacgtgc tcggcaacca
     1321 cgagtactac ggcggggcga tgcccgcgca cctcgaaacc atgaaacagc aggcggccgg
     1381 cagcaatgtt cgcgtgctgg aaaacgaggc gctcgacatc ggcggcgtga ccttcctggg
     1441 ttgcacactg tggaccgatt tcgcgcttta cggcaaagca ccgctgcacg gctttgccgc
     1501 cagacggttg gtgaccgact atcgcattat tcgcgtcagc ccttcgttcc atcggttgcg
     1561 gccgaaacat acggccgagc tgcaccggca atcggcgcgc tggctcgccg aaaccgcccg
     1621 ccgctacgac ggcgaaaaca cggtcgtgat tacgcatcac gcgccgagca tgcgctcgct
     1681 gccggacgac tttcgcggca accccgtcag cgccgcctac gcgtcgaacc tcgaccggct
     1741 ggtggaggag tgcggcgcac agctgtggat tcacggccac ctgcaccggc agagcgatta
     1801 tcggctcggc gacacgcgcg tcgtctgcaa tccgcggggt tacccgaacc agcgcaaccc
     1861 gagcttcgtg ccggacctgg tcatcgagat ctgaggcgcc gatcgcgctc ggtgcggcga
     1921 aggaaatccg ttagaatcga gttcgtcccg ggagatacgt catggccgaa tccgtctacg
     1981 cactcaacct gttcgacatc accgaccgcg aggagtatct cgccta
//
LOCUS       DATCZV010000529         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_255053, whole genome shotgun sequence.
ACCESSION   DATCZV010000529 DATCZV010000000
VERSION     DATCZV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255053"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VIW27_11980"
     CDS             complement(<1..658)
                     /locus_tag="VIW27_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012600060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5740436.1"
                     /translation="MTTLSTRLTHFANPAAGRQPYPAELAAITSLAQAREAMDVICGW
                     PGYAETPLYSLDALAERIGVGGIRYKDESQRFQLKSFKALGGAYAVARQLQQAIGAQT
                     GVAVGIDDLLTGRYRELTADLVISCATDGNHGRSVAWGCRMFGCQCIIYIHRDVSAGR
                     QQAIEAFGAEVVRVDGNYDVSVRQAARDAEAHGRIIVSDTSYPGYMEIPRDVALGYTV
                     M"
     gene            894..>2243
                     /locus_tag="VIW27_11985"
     CDS             894..>2243
                     /locus_tag="VIW27_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5740437.1"
                     /translation="MPVVQQRGVESGLDADRFNAAAMLAADWYWEMDSELRFTFQSAE
                     FETITGIPVTEVIGKTREEAFAGRIDDIDKWGRKADSLRRHADYSMLWTLRRPDGETR
                     LLRTLGKPQFDAWGRFEGYRGIGCDISEVLGIERPETGGADRYRQVIEQVTELFAIVD
                     GEGRIRFLNSAVEKILGFTVVELEAREDGSHALADERVPIRQALREALERPGHAVSGV
                     YRYRHRDGSTRVLEMTRCGIAGLDADGKSAVLLHGRDVTERNNAEQALRETEERLRDF
                     AETGADWFWEQDAELRFTYLSGHNSDFDLLDADGIIGKTREEIIKDCDFDSEKWRQLT
                     ATLEAREPFNNFEYSLPRRDGSSVMVRISGKPRFDEQGEFIGYRGTGIDITESHLLSQ
                     QLNYQATHDPLTGLVNRREFEQRLSRVVRSCREDNSEHALCYIDLDQFKVVNDTCGHD
                     "
BASE COUNT          455 a          671 c          726 g          391 t
ORIGIN      
        1 gcatcaccgt gtagccgagc gcgacgtcgc gcggtatctc catgtagccg ggataactgg
       61 tgtccgaaac gatgatgcgc ccgtgggcct cggcgtcgcg cgccgcttgc ctgaccgaaa
      121 cgtcgtaatt accgtcgacg cgcacgactt cggcgccgaa ggcttcgatt gcctgctggc
      181 ggcccgcgga gacgtcgcgg tgaatgtaaa tgatgcactg acagccgaac atgcggcagc
      241 cccaggccac cgagcgaccg tggttgccgt cggtagcgca actgatgacg aggtcggcgg
      301 tcagctcgcg atagcggccg gtgagcaggt cgtcgatgcc taccgcaacc ccggtttgcg
      361 cgccgatcgc ctgttgcagt tgacgcgcga cggcgtaggc gccaccgagg gccttgaagc
      421 ttttcagctg gaagcgctgc gattcgtcct tgtaacgaat accgccaacg ccgatgcgct
      481 cggcgagcgc gtccagcgag tacaacggcg tctcggcata gccgggccaa ccgcaaatga
      541 cgtccatggc ttcgcgcgcc tgcgcgaggc tggtaattgc ggcgagctcc gccggataag
      601 gctgcctgcc ggcggcggga ttggcaaaat gggtcagtcg ggtagacagc gtagtcatgt
      661 atatagggct gctaaaaaac cgcaagtcta ccgatttgcg gtgcgcagcg aaatgctcgc
      721 ggtgtctcgc gagctattaa aatttcgccc cgggctggac atctggcgct gaaaacctag
      781 actaggcgat tgcgaacagt caggaatggt tgtcgattaa taataaaaca gaaattcctg
      841 cccatgacgg gcaacaaatt gcgcgatccg acaccgatac cggtgccgaa ggggtgcctg
      901 tcgtccagca gcgcggagtc gaatccgggc tggacgcgga ccgcttcaac gctgccgcga
      961 tgctggcggc ggactggtat tgggaaatgg atagcgagct gcgctttacc ttccagtcag
     1021 ccgaattcga gacgatcacc ggtataccgg ttaccgaggt gatcggcaaa acccgtgaag
     1081 aggcgtttgc cggtcgcatc gacgacatcg acaagtgggg ccgcaaggcc gactccctgc
     1141 gccggcatgc cgattactcg atgctgtgga cactgcgcag gcccgacggc gagacccgcc
     1201 tgctgcgcac gctgggaaaa ccgcagttcg atgcctgggg ccgcttcgag ggctaccgcg
     1261 gcatcggctg cgacatttcc gaggtgctgg gaatcgagcg tccggagacc ggcggcgctg
     1321 atcgctatcg ccaggtcatc gagcaggtta cggaactgtt cgccatcgtc gacggcgagg
     1381 gcaggattcg cttcctgaat tcggcggtgg aaaaaatcct cggtttcacc gtcgtcgaac
     1441 tggaagcgcg cgaggacggc agccatgcgc tcgccgacga gcgcgtgccc attcggcaag
     1501 cgctgcgcga ggccctcgag cggcccgggc atgcggtcag cggtgtctac cgctatcgcc
     1561 accgagacgg ttcgacgcgg gtactcgaaa tgacgcgctg cgggatcgcc ggtctcgatg
     1621 ccgacggcaa gtccgcggtg ttgctgcacg gtcgcgacgt gaccgagcgc aacaacgccg
     1681 aacaggcact gcgcgagacc gaggagcgac tcagggattt cgccgaaacc ggcgccgact
     1741 ggttctggga acaggacgcc gagctgcgct ttacctacct gtcggggcac aacagcgatt
     1801 tcgacttgct ggatgccgac ggaatcatcg gcaagacgcg cgaagaaatc atcaaggatt
     1861 gtgatttcga cagcgaaaaa tggcgccagc tcacggctac gctcgaggcc cgcgagccgt
     1921 tcaacaattt cgaatacagt ttgccgcgcc gcgacgggtc gagcgtgatg gtgcgtatca
     1981 gcggcaagcc gagattcgac gaacagggcg agtttatcgg ttatcgcggc accggcatcg
     2041 atatcaccga atcccatctg ctgtcgcagc agctcaacta ccaggccacc cacgatccgt
     2101 tgaccggtct cgtcaaccgg cgcgaattcg aacagcgcct gtcgcgggtg gtgcgctcgt
     2161 gccgggaaga caatagcgaa catgcactgt gctacataga cctcgaccag ttcaaggtcg
     2221 tcaacgatac ctgcgggcac gat
//
LOCUS       DATCZV010000530         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_678277, whole genome shotgun sequence.
ACCESSION   DATCZV010000530 DATCZV010000000
VERSION     DATCZV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678277"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..774
                     /locus_tag="VIW27_11990"
     CDS             4..774
                     /locus_tag="VIW27_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5740438.1"
                     /translation="MGYFAQHQLELLDPDSSPVDHLRDVAPKDREQDHRNYLGRFGFS
                     GARIFEPVAPFSGGEKARLVLALMIRRAPNLLLLDEPTNHLDLEMRQALSVALIEYTG
                     ALVVISHDRHLLKSVCDELLIVHDGIVDRFDRSLDDYAAWLREQEEKSGKGVADTNED
                     AAKSANKKQQRRDQADLRQRLKPLTDKIRKVEKALAENRAILSDLETGLADESLYTDH
                     DRQDELTELVKKQATTKAAIEALEWEWLEATEKLEQAR"
     gene            771..1229
                     /locus_tag="VIW27_11995"
     CDS             771..1229
                     /locus_tag="VIW27_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY5740439.1"
                     /translation="MKIWVDADACPVVVREILFRAAARTGVQVTLVANQPLSVPKAKN
                     INTLQVPGGFDVADDEIVKRVQAGDLVITSDIPLAAEVIDKGGYALSPRGELHTRENI
                     GAKLNMRDFMDTMRSSGVEMSGGPAAYSQRDKQDFANNLDRLLTRHASQA"
     gene            1393..1860
                     /locus_tag="VIW27_12000"
     CDS             1393..1860
                     /locus_tag="VIW27_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5740440.1"
                     /translation="MSFTINSRKVSFDGEADTPLLWVIRDHLKLTGTKFGCGSGQCGA
                     CTVHINGKARRACVTRVSAVAGREITTIEGLAAADDRLHPVQQAWIEHDVPQCGYCQA
                     GQIMAVADFLERFPKPTDADIDKNITNLCRCGTYVRIRKAIHRAAEIAAGDKS"
     gene            1857..>2545
                     /locus_tag="VIW27_12005"
     CDS             1857..>2545
                     /locus_tag="VIW27_12005"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5740441.1"
                     /translation="MSARTEGMEFTRRQVLIAGASVAGSLVIGLPAFAWEETGERKIG
                     FFVQINPDGKVIIGSKDPEIGQGLRTAVPMMVAEELDVDWADVSIEQMPLGLVKTAEG
                     FAWKYGGQGVGGSTGLTGNWEYMRQVGADARRQLIHAAASRLGVDAADCTTRPGFVVC
                     ESRGAEIPYSELVVAAAALPLPEEPAPLKAMSDYRIIGTDRNTIDALDIVTGRTKYGI
                     DTQQPDMRYAV"
BASE COUNT          583 a          732 c          801 g          429 t
ORIGIN      
        1 aaaatcggct acttcgcgca gcaccagctc gagctgctgg atcctgatag cagcccggtc
       61 gatcatctgc gcgatgtcgc ccccaaagac cgcgagcagg atcatcgcaa ctacctgggc
      121 cgtttcggct tcagtggcgc acgcatcttc gagccggtcg cgccattttc cggcggcgag
      181 aaagcgcgcc tggtgctggc gctgatgatt cgcagggcgc ccaacctgtt attgctcgac
      241 gagccgacca accacctcga cctcgaaatg cgccaggcat tgagcgtcgc gctgatcgaa
      301 tacacaggcg cgcttgttgt tatttcccat gaccgccacc tgctgaaaag tgtgtgcgat
      361 gaactgctaa tcgtgcatga cggcatcgtc gaccgcttcg atcgcagcct tgacgattac
      421 gcggcctggc tcagggagca ggaagaaaag tcagggaagg gagtggccga cacgaatgag
      481 gatgcagcga agagcgccaa caaaaagcag caacggcgcg accaggcgga tttgcgtcag
      541 cgcctgaaac cactcacgga caaaatccgc aaggtcgaaa aagcgctggc cgaaaatcgc
      601 gcaatactga gtgatctcga gacgggcctt gccgatgaat cgctgtacac ggaccatgat
      661 cgacaggacg agttgactga gctggtaaag aagcaggcga caactaaagc agccatcgag
      721 gcgctcgaat gggagtggct ggaagccacc gagaagctgg agcaggcgcg atgaaaatct
      781 gggtcgatgc cgatgcctgc ccggtggttg tcagggaaat tctgttccgg gcggccgcaa
      841 ggaccggggt gcaagtgacg ctggtggcaa accagccatt gagcgtgccg aaggcaaaaa
      901 acatcaacac actgcaggtg ccgggaggct tcgacgtcgc ggacgatgaa atcgtcaaac
      961 gcgttcaggc cggcgacctg gttataacga gcgacattcc gcttgcggcg gaggtcatcg
     1021 acaaaggtgg ctacgcgctg agccctcgcg gcgaattgca cacgcgtgaa aacatcggcg
     1081 ccaaactcaa tatgcgtgat ttcatggaca cgatgcgctc gagcggcgta gagatgagtg
     1141 gcgggccggc cgcttacagt cagcgcgaca agcaggactt cgccaataac ctggaccgct
     1201 tgctgaccag gcatgccagc caggcatagg gcgcttgccg ctatactgaa atagcagcga
     1261 agcgtaaccc gctatcaatt gccgctgcag cgttattgct agtgcgactc ggcgaccgtc
     1321 gaacttaaga tgcgggtgcc ggtctgataa gctcacaacc attcgacaca ataagaaggc
     1381 ggaccgatca tgatctcgtt cacgatcaat tcccggaaag tctcattcga tggcgaagcc
     1441 gacaccccgt tgctctgggt cattcgcgat cacctcaaac tgaccggcac caagtttggt
     1501 tgcgggagcg gtcagtgcgg cgcctgcacc gttcacatca atggcaaagc gcgacgcgcc
     1561 tgtgtaacga gggtttcagc ggttgccggc cgcgagatta cgacgatcga gggcctcgcc
     1621 gcagcggatg acaggctgca cccggtacag caggcctgga tcgagcacga cgtgccgcaa
     1681 tgcggctact gccaggccgg ccagatcatg gccgtcgccg attttctcga gcggtttcca
     1741 aagccgacag acgcggacat cgataaaaac atcacgaacc tctgtcgctg tggaacctat
     1801 gtgcgcattc gcaaggccat tcaccgtgcg gccgaaatcg cggcaggaga caaatcatga
     1861 gcgccagaac agaaggaatg gagttcacga ggcggcaggt gctcatcgcc ggcgcgagtg
     1921 tagcgggcag cctggtgatc ggattaccgg cgtttgcctg ggaagaaacg ggcgagcgca
     1981 agatcggctt tttcgtgcag ataaaccctg acggcaaagt catcatcggc agcaaggacc
     2041 cggagatcgg ccaggggctg cgtaccgcgg tcccaatgat ggtcgccgag gaactcgacg
     2101 tcgactgggc cgatgtgagc atcgagcaga tgccgctcgg tctcgtcaaa acggcggaag
     2161 gcttcgcctg gaagtacggt ggccagggcg tcggcggcag cactggtctg accggcaact
     2221 gggagtacat gcgccaggtc ggcgcggacg cgcgccggca actgattcat gcggcggcat
     2281 caaggcttgg cgtcgacgct gcggactgta cgacgaggcc gggcttcgtc gtctgcgagt
     2341 cgcgcggcgc cgagattccc tacagcgaac tcgttgtagc cgccgcggcg ctgcccctgc
     2401 cggaagaacc ggcgccactg aaagccatga gtgactatcg catcatcggc accgacagaa
     2461 acacgatcga cgcactcgat atcgttacgg gcaggacgaa gtacggcatc gatacgcagc
     2521 agcccgatat gcgctacgcg gtgat
//
LOCUS       DATCZV010000531         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_307372, whole genome shotgun sequence.
ACCESSION   DATCZV010000531 DATCZV010000000
VERSION     DATCZV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307372"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VIW27_12010"
     CDS             complement(<1..995)
                     /locus_tag="VIW27_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5740442.1"
                     /translation="MQHRYQIIQADWTVTVNADFEVLSDFAVVVEDNRIRELLAADEV
                     EQLDCYARAEKIRLPGCALLPGLVNSHTHASMSLFRGLADDLPLMTWLTEHVWPAEAR
                     WVDAQFTIDGFRLAAAEMIRTGTTCLSDMFYFPDAAAHCSQEIGMRSVIGLIVLDFPS
                     AWAQNADEYLNKALAVHDEIRELPLVGSALAPHAPYTVSDEPLRKIAMYSNELDVPVH
                     MHVHETAGEVAEALQKTGLRPLERLDRLELLNPNLVAVHMTELDEFEIERIAEAGVNV
                     AHCPESNLKLASGICPLARLLEGGVNVCLGTDGAASNNDLDLLGEMRSAALLAKG"
     gene            1222..>2752
                     /locus_tag="VIW27_12015"
     CDS             1222..>2752
                     /locus_tag="VIW27_12015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEY5740443.1"
                     /translation="MADIAKEVFPVNLEDEMRQSYLEYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMSVLGNDYNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFAMRHML
                     VDGQGNFGSVDGDSPAAMRYTEVRMSKIAHELLADLDKETVDFLPNYDESEQEPLVLP
                     TKVPNLLINGSSGIAVGMATNIPPHNLREVVDACILLVDDADADMEQLMQCIPGPDFP
                     TAGFINGARGIREAYRTGRGKIYLRARTHFEEDKSGRQIIIVTELPYQVNKANLLAKI
                     AELVKEKRLEGISGLRDESDKDGMRMVIELRRGEIADVVLNNLYKQTQMQNVFGINMV
                     ALVNGQPRLLNLRQILQAFIVHRREVITRRTVFDLRKARDRAHLLEGLAVALSNIDEI
                     IALIKQSASPAVAKAALLEKPWQPGVVLEMLRRTGAESSRPEGLAVEFGLYEERYRLT
                     DKQAQAILDLRLHKLTGLEQDKILSEYREILEKIEDLLDILNRDERLKQEIRRELEEV
                     RD"
BASE COUNT          546 a          861 c          860 g          485 t
ORIGIN      
        1 aagcccttgg ccagcagggc cgcgctgcgc atctcgccga gcaggtcgag gtcgttgtta
       61 ctggccgcgc cgtcggtgcc gaggcagacg ttgacaccgc cctcgagcag ccgcgcgagc
      121 gggcagatgc cgctggcgag cttgagattg gattccgggc agtgggcgac gttgacgccg
      181 gcctcggcga tgcgttcgat ctcgaattcg tcgagttcgg tcatgtgcac cgcgaccagg
      241 ttcggattca gcagctcgag ccggtcgaga cgctcgagcg gacgcaggcc ggttttctgc
      301 agcgcctcgg cgacctcgcc ggcggtttcg tgcacgtgca tgtgcactgg cacgtcgagt
      361 tcgttgctgt acatcgcgat cttgcgcagc ggctcgtcgg agaccgtata gggggcgtgc
      421 ggcgcgagcg cgctgccgac cagcggcagt tcgcgaattt cgtcgtgcac ggcgagcgcc
      481 ttgttgaggt attcgtcggc gttttgcgcc caggcgctcg gaaaatcgag cacgatcagg
      541 ccgatcacac tgcgcatgcc gatctcctgc gagcaatgcg ccgccgcgtc gggaaagtag
      601 aacatgtcgg agaggcaggt ggtgccggtg cgaatcattt ccgccgccgc caggcgaaat
      661 ccgtcgatcg tgaactgggc atccacccag cgcgcctcgg ccggccagac atgctcggtc
      721 agccaggtca tcagcggcaa gtcgtcggcg aggccgcgaa acaggctcat cgaagcgtgc
      781 gtgtggctgt tgaccagtcc cggcagcagg gcacagccgg gcaggcgaat cttttccgcc
      841 ctggcgtagc aatccagctg ctcgacctcg tcggcggcga gcagttcgcg aatgcgattg
      901 tcctcgacga ccacggcgaa atcggacaga acctcgaagt ccgcgttgac ggtgacggtc
      961 cagtctgcct ggatgatctg gtagcgatgc tgcatagccg aaccttacaa gatttgtgcc
     1021 gccgttgaaa cgagaattag ccgcatcacg acggcgcggg ccagactcgg cggaaaaacg
     1081 tcgaaatccc ggaaatccgc ttaaattcgc gctggagccg cgatcttatc agtagtcctt
     1141 accccacggt ttatgataaa atcccgcaga tcgaaaataa atcctcccta cgtcgttttt
     1201 taccgttgag tcccaagttt tatggctgat atcgccaagg aagttttccc ggtcaatctc
     1261 gaagacgaga tgcgccagtc atacctcgaa tacgcgatga gcgtgatcgt cggccgcgcg
     1321 cttccagacg tccgcgacgg cctcaagccg gttcaccgcc gggtgctgta cgcgatgagc
     1381 gtgctcggta acgattacaa taagccgtac aagaagtcgg cgcgcgtggt cggcgacgtg
     1441 attggtaagt atcaccccca cggcgacacc gccgtttacg acaccatcgt gcgcatggcg
     1501 caacccttcg cgatgcgcca catgctggtc gacggccagg gcaatttcgg ctcggtggac
     1561 ggcgattcgc ccgcggcgat gcgctacacc gaggtgcgca tgtcgaagat cgcgcacgaa
     1621 ctgcttgccg atctcgacaa ggagaccgtc gattttctgc ccaattacga cgaatccgaa
     1681 caggagccgc tggtcctgcc gaccaaggtg ccgaacctgt tgatcaacgg ctcctccggc
     1741 atcgcggtcg gcatggccac caatattccg ccgcacaacc tgcgcgaggt ggtcgacgcc
     1801 tgcattctgc tggtcgacga tgccgacgcc gacatggagc agttgatgca gtgcattccg
     1861 ggccccgatt ttccgacggc cggcttcatc aacggcgcgc gcggcatacg cgaagcctac
     1921 cgcaccggcc ggggcaagat ctacctgcgc gcccgcactc acttcgagga agacaaatcc
     1981 gggcgccaga tcatcatcgt caccgagttg ccctaccagg tgaacaaggc caacctgctc
     2041 gccaagatcg ccgagctggt caaggaaaaa cgcctcgagg ggattagcgg gctgcgtgac
     2101 gaatccgaca aggacggcat gcgcatggtg atcgaactgc gccgcggcga aattgccgac
     2161 gtggtgctca acaacctgta caaacagacc cagatgcaga acgtgttcgg catcaacatg
     2221 gtcgcgctgg tcaacggcca gccgcggctg ctcaatctgc ggcagattct gcaggcattc
     2281 atcgttcacc gccgcgaagt cattacccgc cgcacggtgt tcgacctgcg caaggcgcgc
     2341 gatcgcgccc acctgctcga aggcctcgcg gtggcgctgt ccaacatcga cgagatcatc
     2401 gcgctgatca agcaatccgc aagcccggcg gtggccaagg cggcgctgct ggaaaagccc
     2461 tggcagccgg gcgtggtgct ggaaatgctg cggcgtaccg gcgccgaatc ctcgcgcccc
     2521 gaggggctgg ccgtggagtt cgggctctac gaagagcgct accggctcac tgacaagcag
     2581 gcccaggcga tccttgacct gcggctgcac aagctgaccg gcctcgagca ggacaagatc
     2641 ctcagcgagt accgcgaaat cctcgaaaag atcgaggact tgctcgatat cctgaatcgc
     2701 gatgagcgcc tcaagcagga aatacgccgc gaactcgagg aggtccgcga cg
//
LOCUS       DATCZV010000532         3630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_574244, whole genome shotgun sequence.
ACCESSION   DATCZV010000532 DATCZV010000000
VERSION     DATCZV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574244"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1378
                     /locus_tag="VIW27_12020"
     CDS             68..1378
                     /locus_tag="VIW27_12020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5740444.1"
                     /translation="MSFRLAQDRSDNADYPDSYYQATRNRELDLPMLDGSRRADVCIV
                     GGGYTGLSAALHLARRGYQVVLLEARKPGWGASGRNGGQLCSGQRKDQLELTSMVGAD
                     DARRFWELAEAAKQTARQLIAEHRVDCDLKPGIAHPNHKPGYDRESRDYVDFLRREYD
                     YQQIEFLEREAMAELVGSGSYYGGTLDMGAGHLHPLNYALGLAAAALDAGASLYRHSP
                     VTGYHEGKPTRVQTERGEIEADHLVLACNGYLGKLEPRVAGKIMPINNFIIATEPLTR
                     AELKVINPRDVAIADSRFVVNYYRLSADKRLLFGGGENYRSNFPSDIASFVRKPMLGV
                     YPQLASKRIDYAWGGTLAVTLNRMPHFGRLGKHDTYFAQGYSGQGVAMATLAGKLIAE
                     ALAGEAERFDLFGKIPTRSFPGGDLLRWPGMVLGMTYYALRDRL"
     gene            complement(1406..2539)
                     /locus_tag="VIW27_12025"
     CDS             complement(1406..2539)
                     /locus_tag="VIW27_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013695940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY5740445.1"
                     /translation="MKITAIEVYILGGPGEQRPHWVSHFPVPAANELLVVLHTEDGLS
                     GFGLGSSNASLESTAKMFTDGLQDFIVGADALAPERLYRELIAITWQRVAADKGWSKA
                     QLVLAAAAVDIACWDLVGKRAGQPLYRLFGGYRDRVPCYVTCAYYRDGKDLDELRDEM
                     HMLESQGHTAFKAKIGALSLEEDMERLRVVREVIGDDAELMIDVNCGWDLPMALAAVK
                     KLPQVNPRWLEEPMHWYDDRRLLKLLAQNTDIPLSAGESEHSAHGCRALLEEQAISVL
                     QFDCTRMGGFTMGRKLAALAELNHVDVAPHHDCFLHAHLVAASPAGLIVESFTDPQRD
                     PLQAELFADPPRIEDGVLYLKDAPGLGLELRPDTIEKYGRRIT"
     gene            complement(2834..3103)
                     /locus_tag="VIW27_12030"
     CDS             complement(2834..3103)
                     /locus_tag="VIW27_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HEY5740446.1"
                     /translation="MLCHIYRSNLKLDTYLYLVDKDDFSMIPAELLRVFGPPEFSFSF
                     DLTESRELAREEATEVLENLESQGYHLQLQSDELVEEMLARKAIN"
     gene            complement(3192..>3630)
                     /locus_tag="VIW27_12035"
     CDS             complement(3192..>3630)
                     /locus_tag="VIW27_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015066082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY5740447.1"
                     /translation="FGKADLTMALNGALAGLVAITAEPSTPTPLVATLFGALGGVLVV
                     FSIVTLDKLKIDDPVGAISVHGVVGLLGLLLVPITNDGSTFGGQILGAITIFVWVFVA
                     SFIVWFVLKATMGIRVSEEDEYDGVDLSECGMEAYPEFVKGSS"
BASE COUNT          673 a         1177 c         1137 g          643 t
ORIGIN      
        1 cgatttcatc gagcgcgcct ggcaatgcct cgatctgacc cagcaagcca ttgcctgata
       61 tgcggcgatg agtttcaggc tggctcagga tcgcagcgac aacgccgact acccggattc
      121 gtattaccag gcgacgcgca accgagaact cgatctaccg atgctcgacg gctcgcgacg
      181 cgccgacgtc tgcatcgtcg gcggtggcta caccgggctg tcggcggcac tgcatctcgc
      241 caggcgcggt taccaggtgg tgctgctcga agcgcgcaag cccggctggg gcgcctccgg
      301 tcgtaacggc ggccagctgt gcagcggcca gcgcaaggac cagctcgaac tgacctcgat
      361 ggtcggcgcc gacgacgcgc gccgcttctg ggaactcgcc gaggccgcca agcagaccgc
      421 acgccagctg atcgccgagc accgcgtcga ctgcgacctg aagcccggca tcgcccaccc
      481 caatcacaaa cccggttacg accgcgagtc gcgcgactac gtcgactttc tacgccgcga
      541 gtatgactac cagcaaatcg agttcctcga acgcgaggca atggccgaac tggtcggcag
      601 cggcagttat tacggcggca cgctggacat gggcgcgggc cacctgcacc cgctcaatta
      661 cgcgctcggc ctggcggccg cggcgctcga cgccggcgcc agcctttacc gtcacagccc
      721 ggtgaccggc tatcacgaag gcaaaccgac gcgcgtgcag accgagcgcg gtgagatcga
      781 ggccgatcac ctggtgctcg cctgcaacgg ctatctcggc aagctcgagc cgcgcgtggc
      841 cggcaagatc atgccgatca acaacttcat cattgccacc gagccgctga cccgcgctga
      901 actgaaggtg atcaacccgc gcgacgtggc gatcgctgac tcgcgtttcg tcgtcaacta
      961 ttaccggctg tcggccgaca agcgcctgct gttcggcggc ggtgaaaact accgcagcaa
     1021 ttttccgtcc gacatcgcca gcttcgtgcg caagccgatg ctcggggtgt atccgcaact
     1081 ggcaagcaaa cgcatcgact acgcctgggg cggcacgctc gcggtgacgc tgaaccgcat
     1141 gccgcacttc ggccgcctcg gcaagcacga tacctacttt gcgcagggct attcgggtca
     1201 gggcgtggcg atggcgacgc tggccggcaa gctgatcgcc gaggcgctcg ccggcgaggc
     1261 ggagcgtttc gacctgttcg gcaagattcc gacgcgcagc tttccgggcg gtgacctgct
     1321 gcgctggccg gggatggtac tcggaatgac ctattatgcg ttacgagatc gattgtaagg
     1381 cgtcgagggt accgggtctc gctgactagg tgatgcggcg gccgtatttt tcgatcgtat
     1441 ccggccgcag ttcgagaccc aggccgggcg cgtccttgag ataaagcact ccatcctcga
     1501 tgcgcggcgg gtcggcgaac aactctgcct gcagcggatc gcgttgcggg tcggtaaagg
     1561 attcgacgat cagtccggcg gggctcgccg ccaccagatg tgcgtgcaga aagcagtcgt
     1621 ggtgcggcgc gacgtcgacg tggttcagct ccgccagcgc cgccagcttg cggcccatcg
     1681 tgaagccgcc catgcgcgtg caatcgaatt gcagtacgct gatcgcctgt tcctcgagca
     1741 acgcgcggca accgtgggcg ctgtgctcgc tttcacccgc cgacagcgga atatcggtat
     1801 tctgcgccag caacttcagc agccgccgat cgtcgtacca atgcatcggt tcttcaagcc
     1861 agcgaggatt gacttgcggc agcttcttga ccgcggccag cgccatcggc aggtcccagc
     1921 cgcaattgac gtcgatcatc aactcggcgt catcgccgat gacctcgcgc accacccgca
     1981 gccgctccat gtcttcctcg agagagagcg caccgatctt ggccttgaac gcggtatgcc
     2041 cctgcgactc gagcatatgc atttcatcgc gcaactcgtc gaggtccttg ccgtcgcggt
     2101 aataggcgca ggtgacgtaa cagggcaccc ggtcgcggta accgccgaac agccggtaaa
     2161 gcggctggcc ggcacgcttg ccaaccaggt cccagcaggc gatatcgacc gccgccgcgg
     2221 ccagcaccag ttgcgccttg ctccagccct tgtcggcggc gactcgctgc caggtgatcg
     2281 cgatcagctc gcggtaaagc cgctccgggg ccagcgcgtc ggcgccgacg atgaagtcct
     2341 gcaagccatc ggtgaacatt ttcgcggtcg actcgagcga ggcgttgctg ctgccgaggc
     2401 caaaacccga caagccgtcc tcggtgtgca gtaccaccag gagttcgttg gccgcgggca
     2461 ccggaaagtg gctcacccag tgcggccgct gttcgcccgg gccgccgaga atatagactt
     2521 cgattgcggt aattttcatt ttttacaggc tgctcgagga ttgaatattt gcaccgatta
     2581 tgccgcagcg aacgccggaa tgcgcggtga ttatgccgcc cggacccgtc ccggcgcagg
     2641 cgttggttag acgatcgggg tcattctcgc gtaagcggga atctcttcac catcgggaga
     2701 cctcgctggc gcgggggtga cagctccgga gtcattcccg cgcaagcgcg aatcttgcaa
     2761 ccttcaggag gtccccgctt gcgcggggat gacatcgctg gatttctgtg cgttgcacag
     2821 atatccggag atgtcagttg atggccttgc gggcgagcat ctcctcgacc agctcgtcag
     2881 actgcagctg caggtggtaa ccctggcttt cgagattttc gagcacttcg gttgcctcct
     2941 cgcgggcgag ttcgcgcgat tcggtcagat cgaaactgaa gctgaattcg ggcggaccga
     3001 acacccgcag caactcggcg gggatcatcg aaaaatcgtc cttgtcgacg aggtagaggt
     3061 aggtatcgag ctttagattg ctacggtaga tgtggcacaa catggcttat cgcgagcttt
     3121 cggggactcc gcgcatggta gcacagaaac gaacccgggg ccggccgctg ccggccccgg
     3181 ttcgatcgag cttaactgct tcccttgacg aactcggggt aggcttccat accgcactcc
     3241 gacaagtcga cgccgtcgta ctcgtcttcc tcgctgacgc ggatgcccat ggtcgccttg
     3301 agcacgaacc agacgatgaa gctggcaacg aaaacccaga cgaaaatggt gatcgcaccg
     3361 aggatctgac cgccgaaggt cgagccgtcg ttggtgatcg gcaccagcag caggccgagc
     3421 aggccgacga caccgtgtac cgagatcgca ccgaccggat cgtcgatctt cagcttgtcg
     3481 agggtgacga tgctgaacac caccagcacc ccgcccagcg caccgaacag cgtcgccacc
     3541 agcggcgtcg gggtcgaggg ctcggcggtg atcgccacca ggccggcgag cgcaccgttg
     3601 agcgccatcg tcaggtcagc cttgccgaac
//
LOCUS       DATCZV010000533         6248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_148990, whole genome shotgun sequence.
ACCESSION   DATCZV010000533 DATCZV010000000
VERSION     DATCZV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148990"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VIW27_12040"
     CDS             complement(<1..517)
                     /locus_tag="VIW27_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5740448.1"
                     /translation="MLIKIGGGEQIDLDAVIADLADLAPPYLIVHGANVLRDQLGARL
                     GIEKTVLTSVSGYSSVFSDDDAIDVILMSYAGLRNKRIVELCQRRGIDAVGLTGLDGR
                     LVQGERNRGIRVREGGKTLIRRDLSGKPRQINTALLGLLLDNGYTPVLTIPIADEKGF
                     AINSENDDIVAL"
     gene            complement(517..1152)
                     /locus_tag="VIW27_12045"
     CDS             complement(517..1152)
                     /locus_tag="VIW27_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740449.1"
                     /translation="MCRILYARADAPFEIAELLRPFAELSRDSREYQGHGWGCAWRDA
                     GGWRQYHDIRPVWEDDPQQFGSTDLLLVHARSAFRDEGIRVENNMPFSDGRSMFIFNG
                     ELRGVRVRSEGRIGAEKIYNYIRRFDRGDLCAAVSKAVDIIHRRSAYVRAMNLILADG
                     ERGCLSTSFGEDPEYFQMHEKRQAGRHLLCSEPFPADPGWSPIANHTTLPL"
     gene            complement(1427..2449)
                     /gene="argC"
                     /locus_tag="VIW27_12050"
     CDS             complement(1427..2449)
                     /gene="argC"
                     /locus_tag="VIW27_12050"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY5740450.1"
                     /translation="MIRVSIVGGSGYTGGELLRILLFHPGVEISQVTSERHAGKPVAS
                     LHPNLRKLCAHRFIPSAELKKCDVLFLCLPHGQLMSRLVEFELHAGMLIDLSADFRLQ
                     DGADYETWYGKPHTCAERLDDFVYGIAELHREQLRGAKRISGAGCNATATILALLPLY
                     RAGVVESAVVEVKAGSSQGGNSPGPGSHHPERSGALRSYKPTAHRHVAEIRQALGADN
                     IHFSATSVEMVRGILATCHVFLNRDLEEREVWKLYRGAYAEEPFIRIVKERSGIHRYP
                     EPKILAGSNYCDIGFERDPHSNRLVVISALDNLVKGAAGQAVQAFNIAQGFDENTGLE
                     FPGLHP"
     gene            complement(2446..3282)
                     /gene="lysX"
                     /locus_tag="VIW27_12055"
     CDS             complement(2446..3282)
                     /gene="lysX"
                     /locus_tag="VIW27_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEY5740451.1"
                     /translation="MRIGLLHSLIRKEEKLLLDAFAGAGVEPLMIDDRKLILDFHRAP
                     EIDLLVERSINHSRALHALRLFESAGVRCINSHRVAQICGDKLLTTAALKDAGVPQPE
                     CRVAFTEESALEAIEELGYPVVLKPAVGSWGRLLSKINDRDAAETVLEHKTVLGSYHH
                     SIFYIQQYIEKQNRDIRSFVVGDDCVAAIYRSSDHWITNTARGAHASKCEVTPEVAQI
                     SLAAAATVGGGIVAVDLFESADGLLVNEVNYTMEFRNSIDTTGVDIPRVVADYVIGQA
                     RQ"
     gene            complement(3279..3488)
                     /gene="lysW"
                     /locus_tag="VIW27_12060"
     CDS             complement(3279..3488)
                     /gene="lysW"
                     /locus_tag="VIW27_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HEY5740452.1"
                     /translation="MHKILRLPLKQQGNTMGECPICAAQVHVSTGTIPGELLVCDDCG
                     AELEVIETDPVELAEAPSAEEDWGQ"
     gene            3566..4018
                     /locus_tag="VIW27_12065"
     CDS             3566..4018
                     /locus_tag="VIW27_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY5740453.1"
                     /translation="MRNTFEEMRRRLMLALAAVMIAPGRGRAAAAAEYAAGANAEAFV
                     ARAFELMRAAIDSGDQGYGAVVVRDGRIIGEAASRVVLDGDPTAHAEMEAIRDAARRS
                     GSRDLGGCTLYSSSPPCPMCEAAAYWAGIDRLVHGSPARDGGRPRLCG"
     gene            complement(4015..4290)
                     /locus_tag="VIW27_12070"
     CDS             complement(4015..4290)
                     /locus_tag="VIW27_12070"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY5740454.1"
                     /translation="MTNPTIADNTPVKIALEQGKKYFFCVCGLSSKQPFCDGSHQGGE
                     FTPLAFTCDESKDYFLCRCKHSGNKPYCDGSHKQFGDDQVGRPAADT"
     gene            4371..5114
                     /locus_tag="VIW27_12075"
     CDS             4371..5114
                     /locus_tag="VIW27_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740455.1"
                     /translation="MPDTLVIQSHRRPLSCGWFEPCLESVAGWAESRGFDYRFIDDDI
                     FDLVDSALRERFEARPAIISDLARLKALQQGLEQGYRRVVWCDADLLIFAPRDLQLPN
                     SAFALGREVWIQPAADGGWRSYFRVHNALLMFARGNAFLDFYADTAERLLRRNQGRMP
                     PQFIGPKLLAALHNIAQFPVIESAAMLSPPVMRDLLAGGGDALALFRRKSVEPPAAAN
                     LCASLVHGEGFTAADMQQLIDRLLTTGIA"
     gene            complement(5145..>6248)
                     /locus_tag="VIW27_12080"
     CDS             complement(5145..>6248)
                     /locus_tag="VIW27_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5740456.1"
                     /translation="NVASRDAAAIARMQRAGAIFIGKNNLHEFAYGATGENSFYGTPP
                     NPWDSSRLAGGSSSGSAAALAYGLCAAALGTDTGGSVRAPAALCGLVGFKPTYGRIAT
                     EGVIPYSWSLDHVGSLTRSAADAQFLFAALARDSAAPDPGDLRGLEFGIADSFFFERC
                     DSEILAAVERLMDFLQGAGARLVPVTMPSMEYTRSVSLTVQMPEALCYHNRYLATRGE
                     LYSPDFRAGLALGQCLLAEQYIRALRLIETYRRDLQRVFDSVDLLLTPATPVIAPAIG
                     SVEVSVDGHSEPVGNAITRYTSFFNMTGHPAITFPCGMHGSGLPIAAQLVGRHYADEN
                     LLEITALIEREFGFTLPHPPIDLPTTSTGPKLD"
BASE COUNT         1100 a         1907 c         2068 g         1173 t
ORIGIN      
        1 gcagcgcgac gatatcgtcg ttttcggaat tgatcgcgaa acccttttcg tcggcgatcg
       61 ggatcgtcag caccggagtg tagccattat cgagcagcag gccgagcagc gcggtgttga
      121 tctgccgcgg cttgcccgac aggtcgcgcc gaatcagtgt cttgccgccc tcgcgcacgc
      181 gaattcccct gttgcgctca ccctgcacca ggcggccgtc gagaccggtg aggccgactg
      241 cgtcgatacc gcggcgctgg cacagctcga cgatgcgctt gttacgcagc cccgcgtaac
      301 tcatcaggat gacatcgata gcatcgtcgt cggaaaatac gctgctatag cccgacaccg
      361 aggtcagcac ggttttctcg atgccgaggc gcgcgccaag ctggtcgcgc agcacattgg
      421 cgccatgcac gatcaggtag ggcggagcga ggtcggcgag gtcggcgatg acggcgtcga
      481 gatcgatctg ctcgccgcca ccaattttta tcagcatcat aagggcaggg tcgtgtggtt
      541 tgcgatcggc gaccagccag ggtccgccgg aaaaggttcc gagcataaca gatgacggcc
      601 cgcctggcgc ttttcgtgca tctggaagta ttccgggtcc tcgccgaagc tggtcgacag
      661 gcagcctcgc tcgccgtcgg cgagaatcag gttcatcgcc cgcacgtagg cgctgcgtcg
      721 atgaatgata tcgaccgcct tgctgaccgc cgcgcacagg tcgccgcggt cgaagcggcg
      781 aatgtagttg taaatctttt cggcgccgat tcgaccttcc gagcggacgc gcacaccgcg
      841 caattcgccg ttgaagatga acatcgagcg gccgtcggaa aacggcatgt tgttctccac
      901 ccggatgccc tcgtcgcgaa atgcgctacg cgcatgcacc agcagcagat cggtgctgcc
      961 gaactgttgc gggtcgtctt cccagacggg acgaatatcg tggtactgtc gccagccgcc
     1021 tgcgtcgcgc catgcacagc cccagccgtg gccctggtac tcgcggctgt cgcgggacag
     1081 ttcggcgaac ggacgcagca gctcggcgat ctcgaacggc gcgtcggcgc gagcatagag
     1141 gatgcggcac atcgcggctg cctagcccgc gtcctgcatg caagcagccc gatccggcgc
     1201 aagcaagggg tatggatcac caccggcagg cagatttttc acctgcgtca tcgcaggcgc
     1261 aaaccctttc ggggttgctg tgcagtgctg ttccgcgtgc cgatgcctgg tactcaaatc
     1321 acagggatac cggcgatact gcggccggta cctgatactt ttgagcgagt ctctgcaatt
     1381 cataacggtt gtcgagagtt tgcgacgctc gccagcaagc cgcgggctag ggatgcaggc
     1441 cgggaaactc gagcccggta ttttcgtcga aaccctgcgc gatgttgaac gcctgcactg
     1501 cctggccggc ggcgcccttg acgaggttgt cgagcgccga gatcacgacc agccggttgc
     1561 tgtgcggatc gcgctcgaag ccgatgtcgc agtaattgct gccggcaagt atcttgggtt
     1621 ccgggtagcg gtgaatgccg ctgcgctcct tgacgatgcg gatgaagggc tcctcggcgt
     1681 aggcgccgcg atacagcttc cagacctccc tttcctcgag atcacggttc aggaacacgt
     1741 gacaggtggc gaggatgccg cgcaccattt cgaccgaagt cgccgaaaaa tgaatattgt
     1801 cggcaccgag tgcctggcgg atttcggcga cgtggcgatg cgcggtcggc ttgtaggagc
     1861 gcagcgcgcc gctcctttcg gggtgatgac ttccggggcc gggcgaattg ccgccctggc
     1921 tggagccggc cttgacctcg accacggccg attcgaccac cccggcccga tacagcggca
     1981 gcagcgcgag gatggtcgcg gtcgcgttgc agccggcgcc ggaaatgcgc ttcgcgccgc
     2041 gcagctgctc gcggtgcagc tcggcgatgc catagacgaa atcgtcgagg cgctcggcgc
     2101 aggtatgggg cttgccgtac caggtttcgt agtcggcgcc gtcctgcagg cgaaaatcgg
     2161 ccgacaggtc gatcagcatg ccggcgtgca gctcgaactc gacgaggcgg ctcatcagtt
     2221 ggccgtgcgg caggcacaga aacagcacgt cgcatttttt gagttcggca ctgggaatga
     2281 acctgtgtgc gcacagcttg cgcaggttcg ggtgcaggct ggcgaccggc ttgccggcgt
     2341 ggcgctcgga ggtcacctgg ctaatctcga ccccgggatg aaacagcagg atgcgcagca
     2401 gttcaccccc ggtgtacccc gaaccgccga cgatggaaac gcgaatcatt gccgcgcctg
     2461 gccgatcaca tagtcggcca ccacccgcgg aatatcgacg ccggtggtat cgatactgtt
     2521 gcggaattcc atcgtgtaat tgacctcgtt gaccagcagg ccgtcggcgg attcgaacag
     2581 gtcgacggcg acgataccgc caccgacggt cgccgccgcg gccagcgaga tttgggccac
     2641 ctcgggcgtg acttcgcact tgctggcatg tgcgccacgc gcggtattgg tgatccagtg
     2701 atcggagctg cggtaaatcg ccgcgacaca gtcgtcaccg accacgaaac tgcgaatgtc
     2761 gcggttttgt ttctcgatgt attgctgaat gtaaaagatc gaatggtgat agctgccgag
     2821 cacggtcttg tgctcgagca cggtttcggc tgcgtcgcga tcgttgatct tcgacaacag
     2881 gcgcccccag gagccgaccg ccggtttcag caccaccgga tagccgagtt cctcgatggc
     2941 ctcgagcgcg gattcctcgg taaaggcgac ccggcactcg ggctgaggca cgccggcatc
     3001 cttgagcgcc gcggtggtca gcagcttgtc gccgcaaatc tgcgccacgc ggtgcgaatt
     3061 gatgcagcgc acgccggcgc tttcaaacag gcgcagcgca tgcagcgcgc gggaatgatt
     3121 gatcgaccgt tcgaccagca ggtcgatttc gggcgcgcga tggaaatcca agatcaactt
     3181 gcggtcatcg atcatcagtg gttcgacgcc ggcaccggcg aaggcatcta gcagcagttt
     3241 ttcttccttg cgaatcagcg agtgaagcag gccgatcctc actgacccca gtcctcttcg
     3301 gcggacggcg cctcggccag ttccaccgga tcggtttcga ttacctcgag ctcggcaccg
     3361 caatcgtcgc agaccagcaa ttcacccgga atggttcccg tagatacgtg aacctgagcc
     3421 gcgcagatcg gacattcccc catcgtattc ccctgttgtt tcagtggcag gcgcagtatt
     3481 ttatgcattt gccggcgcgc ggcacaagcg ggatttgatg cgcgggtgct aaactgtcgg
     3541 cgaattgggc gaacagggtg gcgagatgcg aaacacgttc gaagagatgc gccgcaggct
     3601 gatgctggcg ctggcggcgg tgatgattgc gcctggcaga ggtcgtgccg ccgccgctgc
     3661 cgagtacgcg gcgggcgcca acgccgaggc tttcgtcgcg cgcgcgtttg agctgatgcg
     3721 cgccgcgatc gacagcggcg accagggtta cggcgcggtg gtagtgcgcg acggacgtat
     3781 catcggcgag gcggcaagcc gcgtggtgct cgatggcgat cctaccgcgc atgccgaaat
     3841 ggaggcgatt cgagacgccg cgcggcgcag tggcagccgc gatctcggcg gttgcacgct
     3901 ctactcgtcg tcgccgccgt gcccgatgtg cgaggccgcg gcctactggg ctggcatcga
     3961 tcgcctggta cacggatcgc cggcccgcga cggcggtcgg ccgcggttgt gcggctaggt
     4021 gtccgcggcg ggccggccga cctgatcgtc gccgaactgc ttgtggctgc cgtcgcaata
     4081 gggcttgttg ccgctgtgct tgcagcggca caggaaataa tccttgctct cgtcgcaggt
     4141 aaaagccagc ggggtaaatt caccgccctg atgcgagccg tcgcaaaatg gctgcttgct
     4201 ggagagtccg caaacgcaga aaaagtattt ctttccctgc tcgagcgcga tcttgacggg
     4261 ggtattgtcg gcgattgtcg ggttggtcat cgaggctcct cgttggcgat ctgcaaaggc
     4321 ggctaagata acatgttaaa gtcgagtgct cgacccggat tgactctgcg atgcccgata
     4381 ctctcgtcat ccagtcgcac cgtcgccccc tgagctgtgg ctggttcgag ccctgtctcg
     4441 aatcggtcgc cggctgggcc gaatcgcgcg gtttcgacta tcggtttatc gatgacgaca
     4501 tcttcgacct ggtcgattcg gcgttgcgcg agcggttcga ggcacggccg gcgatcatca
     4561 gcgatctggc gcgcctgaag gcgctgcagc aggggctcga gcagggctat cggcgcgtcg
     4621 tctggtgcga tgccgatttg ctgatattcg ccccgcggga tctgcaactg ccgaattccg
     4681 ctttcgcgct cggccgcgag gtctggatac agccggccgc cgatggcggc tggcgcagct
     4741 actttcgagt ccataatgca ttgttgatgt tcgcgcgcgg caatgcattt ctggatttct
     4801 atgccgatac cgccgagcgt ctgctgcgac gcaaccaggg ccgcatgccg ccgcagttca
     4861 tcggtcccaa attgctggcc gcgctgcaca acatcgcaca attcccggtg atcgaaagcg
     4921 ccgcgatgct gagcccgccg gtgatgcgcg acctgctcgc cggtggcggc gatgcgctgg
     4981 cgctgtttcg tcgcaaatcg gtcgagccgc cggcggccgc caacctgtgc gcgtcgctcg
     5041 tgcatggcga aggcttcacc gcggccgaca tgcagcagct gatcgaccgc ctgctcacca
     5101 ccggtattgc ctgaacccga gtcataaccg aattagaacg ccgtttaatc cagcttcggg
     5161 ccggtcgacg tggtgggcag gtcgatgggg ggatggggca gggtgaagcc gaattcacgc
     5221 tcgatcagtg cggtgatttc gagcaggttt tcgtcggcgt aatggcggcc gacgagttgt
     5281 gccgcgatcg gcagcccgct gccgtgcatg ccgcatggaa aggtgatcgc cggatggccg
     5341 gtcatgttga aaaacgaggt gtagcgggta atcgcgttgc cgaccggttc gctatggcca
     5401 tcgacgctga cttcgacgct gccgatggcc ggtgcgatca ccggagttgc cggcgtcagc
     5461 agcaggtcga cgctgtcgaa aactcgctgc agatcgcgcc ggtaggtttc gataaggcgg
     5521 agggcgcgaa tgtactgttc ggccagcagg cactggccga gcgccagtcc ggcgcggaaa
     5581 tccggactgt acagttcacc acgggttgcc aggtagcggt tgtgatagca aagcgcctcc
     5641 ggcatctgca cggtcagcga tacgctgcgc gtgtattcca tcgacggcat ggtcaccggc
     5701 accagtcgcg cgccggcgcc ttgcagaaaa tccatcaaac gctcgacggc ggcgaggatt
     5761 tcgctgtcgc agcgttcgaa gaagaagctg tcggcaatgc cgaactcgag cccgcgcaga
     5821 tcgcccgggt cgggagccgc cgagtcgcgc gccagcgcgg cgaacaggaa ttgcgcatcg
     5881 gcggcgctgc gcgtcaagct gccgacgtga tccagcgacc acgagtaggg aatgacgcct
     5941 tcggtagcga tgcgcccgta ggtcggtttg aagcctacca gcccgcagag agctgccggt
     6001 gcgcgcaccg agccgccggt atcggtcccc agcgcagcgg cgcagaggcc gtatgccagt
     6061 gccgctgccg agccgctgct ggagccgccg gcgaggcgcg acgaatccca cggattcggc
     6121 ggtgtgccgt aaaacgagtt ttcgccagtg gcgccgtagg cgaattcgtg caggttgttc
     6181 ttaccgataa agattgcacc ggcccgctgc atgcgggcga tcgcggccgc gtcgcgggag
     6241 gcgacatt
//
LOCUS       DATCZV010000534         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_95952, whole genome shotgun sequence.
ACCESSION   DATCZV010000534 DATCZV010000000
VERSION     DATCZV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95952"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VIW27_12085"
     CDS             <1..459
                     /locus_tag="VIW27_12085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740457.1"
                     /translation="LINDIIKWFGFEPIRLLYTEFAVYVGIVYTYLPFMILPLYANME
                     KLDWELLEAAADLGAKPMTTFFTVTLPMTVPGIIAGSLLVFIPATGEYVIPDLLGGGN
                     VLMIGRVLYNEFNANIDWPVASAVAIALLLVLVLPMMLYQHIQSQEAEGK"
     gene            456..1301
                     /locus_tag="VIW27_12090"
     CDS             456..1301
                     /locus_tag="VIW27_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5740458.1"
                     /translation="MNNGRRSRFLLTTLCFGLAFLYVPMVLLVVYSFNKSKLVPVWGG
                     FSTRWYGVLFESEEIWDAVGLSLQVATVNACFATLLGTLAGLALVRFGRFRGRTLFTG
                     MITAPLVMPEVITGLSLLLLFITLKEFIGWPDQRGFTTITIAHITFSMAYVAVIIQSR
                     LAGMAQDLEEAAQDLGAKPLRVLTDITLPLLAPGMLAGWLLSFTLSLDDLIIASFVTG
                     PGATTLPILIYSRIRLGLRPDINALATIIILVVAIGVLVAGYIMLRQQRQRELELRMT
                     AEETP"
     gene            complement(1691..2632)
                     /locus_tag="VIW27_12095"
     CDS             complement(1691..2632)
                     /locus_tag="VIW27_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY5740459.1"
                     /translation="MILRGGSMADSFSLRTRSAQGGTPRLTDWGVDCEYGVLRDVLLG
                     PADNYRWLETSSVSRKSIRRNYEFDAGIARRQHAEMIAAYQSADVRVHLHEPDPALPY
                     QIFARDSSVMTPYGAIITSMANWWRRGENFRAIETYERLGIPIYDYVTAGSFEGGDFN
                     VIEPGAVLIGWEGDEGRSQQQSALQLKDWFEAEGWEVKLADIDPFYVHIDLMVVMLAP
                     KLAAVCLESTDPEVVDWLKSKRIEIVSVPFQDTLDLGCNVVALGNDRVLLPEKSHVLK
                     EKVRAHGLEVFDPDVSMITPGGGGVHCMCQALRRDPG"
BASE COUNT          466 a          858 c          820 g          512 t
ORIGIN      
        1 ctgatcaacg acatcatcaa gtggttcggt ttcgaaccga tacgcctgct gtacaccgag
       61 ttcgcggttt acgtcggcat cgtctacacc tacctgccgt tcatgatcct gccgctctac
      121 gccaacatgg aaaagctcga ctgggaactg ctggaggcgg cggcggatct cggcgccaag
      181 ccgatgacga ccttttttac cgtcaccctg ccgatgacgg tgcccggcat catcgccggc
      241 tcgctgctgg tgttcatccc ggcgaccggc gaatacgtga ttcccgacct gctcggcggc
      301 ggtaacgtgc tgatgatcgg ccgcgtgctg tacaacgagt tcaacgccaa catcgactgg
      361 ccggtggcct cggcagtggc catcgcgctg ctgctggtcc tggtgctgcc gatgatgctc
      421 taccagcata tccagtcgca ggaggccgag ggcaagtgaa caacggtcga cgctcgcgct
      481 tcctgctgac cacgctttgc ttcggcctgg cgtttctcta cgtgccgatg gtcttgctgg
      541 tggtctactc gttcaacaaa tccaagctgg tgccggtatg gggcgggttt tcgacccgct
      601 ggtacggggt gctgttcgag agtgaggaaa tctgggacgc ggtgggcctg agcctgcagg
      661 tggcgaccgt caacgcctgc ttcgcgacct tgctcggcac cctggcgggg ctggcactgg
      721 tgcgcttcgg tcgctttcgc ggccgcacgc tgttcaccgg catgatcacc gcgccactgg
      781 tcatgcccga agtcatcacc ggcctgtcgc tgctgctgct gttcattacc ctgaaggagt
      841 tcatcggctg gcccgaccag cgcggcttca cgacgatcac catcgcgcat atcacctttt
      901 cgatggctta cgtcgcggta atcatccagt cacgactggc gggtatggct caggacctcg
      961 aggaggccgc gcaggatctc ggcgccaagc cgctgcgcgt gctgaccgat atcaccctgc
     1021 cgctgctggc gccggggatg ctggccggct ggctgctgtc gttcacgctg tcgctcgacg
     1081 acctgatcat cgccagcttc gtcaccggcc cgggagcgac cacgctgccg atcctgatct
     1141 actcgcgcat tcgcctcgga ttgcgaccgg atatcaacgc gctggcgacg atcatcatcc
     1201 tggtggtcgc gatcggcgtg ctggtggccg gttacatcat gctgcgccag cagcgccagc
     1261 gcgagctcga actgcgcatg accgccgagg aaacccccta accccaagtt ggggtcagtc
     1321 ccgcgcacgc ggcgcgcggc ccggccgaac gcggcccggc cgaacgcggc ccggccgaac
     1381 gcggcccggt gggaatctta tatatcgcga cggctttaca agatccccgc ttacgcgggg
     1441 atgacctgca aatcaaaggg gtcgatgtcg tttgatccgc tgacggataa tcaaacgggt
     1501 gattcctggc cgcggagcgc ttgattaatc ctggcaaggc gggggcatcg caaagtcgcg
     1561 gtggcaggcc gcacggagta aaggttcgcg aggcgtagca ggccctacgt tgagcgggcc
     1621 tttgcaaccc gatgtgccag ggcacatcgg gggcggcccg tcacagtgcg ctttgcgacg
     1681 tccgcagccg ctagccgggg tcgcggcgca gggcctggca catgcagtgg acgccgccac
     1741 cgcccggggt gatcatcgat acgtcgggat cgaagacctc gagtccgtgc gcgcgaacct
     1801 tctccttcaa cacgtggctc ttttccggca gcagcacacg gtcgttgccg agcgcgacca
     1861 cgttgcaacc gagatcgaga gtgtcctgaa agggtaccga gacgatttcg atgcgtttcg
     1921 atttcagcca gtcgacaacc tcgggatcgg tcgactcgag gcataccgcg gcgagcttcg
     1981 gcgccagcat caccaccatc aggtcgatat gcacgtaaaa cgggtcgata tcggcgagct
     2041 tgacttccca gccctcggcc tcgaaccagt ctttgagctg cagcgccgac tgctgctggc
     2101 tgcggccctc atcgccttcc cagccgatca ataccgcgcc cggttcgatg acgttgaagt
     2161 caccaccctc gaaactgccg gctgtcacgt agtcgtaaat cggaatgccg aggcgttcat
     2221 aggtttcgat ggcacgaaaa ttttccccgc gccgccacca gttcgccatg ctggtgatga
     2281 tcgcgccgta gggcgtcatc accgacgaat cgcgcgcgaa gatctggtac ggcaacgccg
     2341 gatcgggctc gtgcaggtgt actcgcacgt ccgccgactg gtaagccgcg atcatctcgg
     2401 catgctggcg gcgcgcgatc ccggcatcga actcgtaatt gcggcgaatg ctcttgcgcg
     2461 ataccgagct ggtctccagc caacggtaat tatcggcggg gccgagcagc acgtcgcgca
     2521 gcacgccgta ttcgcaatcg acgccccaat cggtaagccg cggcgtaccg ccctgcgcac
     2581 tgcgggtgcg taaactgaaa ctgtcggcca tggaccctcc acgcaaaatc atgtgatcac
     2641 taacaatccc gcgagg
//
LOCUS       DATCZV010000535         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_626183, whole genome shotgun sequence.
ACCESSION   DATCZV010000535 DATCZV010000000
VERSION     DATCZV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626183"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..259)
                     /locus_tag="VIW27_12100"
     CDS             complement(83..259)
                     /locus_tag="VIW27_12100"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5740460.1"
                     /translation="MRKRARRVVWLNPLLNQPGYSPESRGMQAAIPHLDLLAPGADLD
                     SIENVLPQLIEALR"
     gene            331..591
                     /locus_tag="VIW27_12105"
     CDS             331..591
                     /locus_tag="VIW27_12105"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY5740461.1"
                     /translation="MRVALMLPLFFASLLEDFGFYTLAPLFIIGVMYVFGERRPKHMA
                     VITAVLMGFLLLLFVKILYVGLPVGNIRPFYDFGSWVVEMLQ"
     gene            601..2145
                     /locus_tag="VIW27_12110"
     CDS             601..2145
                     /locus_tag="VIW27_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY5740462.1"
                     /translation="METFNNFLQGSITLFSDPINILIFLGGVVGGMLFGAIPGVSMLT
                     LAAILLPFTAHLSAEQGIMLFSVIYCTGVYGGAITAILFNIPGAPENAPTAFDGYPMT
                     KNGQAGKAVGAAVMCSALGGVVSTIIMMAATAPIADWAISAFGPPEIFALVFFGLAVA
                     ATVGAKTFWKGWLSILIGLMVSVVGLDPVGGIERYDFGMPYLLAGIHFIPTILGFFAV
                     SEIFVQAEKKVRGSYEAPKLSVNFPTLAELVAHKVAVVRSIVIGFFCGILPGIGATLA
                     AFMGYNEAVRWSKTPEEFGKGKLEGVISSETANNAATGAAMIPLLALGLPGGALTAMM
                     VGVFQMHDMQPGPLVFLTSADLVWVVFAAMFFANCSILLIGFLETKTIINLLRIPFQF
                     LAPLILLLATVGAYAVRGLTIDIVVMFLAGVIGFFLRRSGYSIAGIVLGVILGSIGEQ
                     TFAQAMQMLHFEWSGFLDRPIAAGLLAVGFITLGASIYGEFFKKSAPTSNGPDSPAES
                     HRSGEG"
     gene            2305..>3045
                     /locus_tag="VIW27_12115"
     CDS             2305..>3045
                     /locus_tag="VIW27_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740463.1"
                     /translation="MMPMDYLDFASDLPVNGRIIFSSAEESEEVMRQYGVDQQLRQSS
                     KGSFQADCTMLETEEAILSADRFAQACLAHFEPPPDTVSILAFRCAGGEFLVSGEKVT
                     EDNLVVVPHGHGTDLVFSNLAGSEGVAIPKTKYSELTEALCPSCAQPEGLQIAKGNPR
                     LLQGIRNSVIRIIAAAQPRDENIDDLVARMVLTANADPWAERPEIFYRKQAKIDIAKQ
                     VQTFIDENYQEQIRIEDLCRATERGVRTL"
BASE COUNT          548 a          912 c          896 g          689 t
ORIGIN      
        1 atgacccgca ccaccgtgac cagcgcgtag ggttcgccag cggtattcag ctcgcgtgcc
       61 agttcctgta cttcgtcgtt tgtcatcgca gtgcctcgat gagttgcggc aaaacgtttt
      121 cgatactgtc gagatcagcg cccggtgcga gcaaatcgag atgcggtatg gccgcctgca
      181 taccgcgact ctcggggctg taacccggtt ggttcaacaa cgggttgagc cacacgacgc
      241 gccgggcgcg ttttcgcaag gtggtgagcc cgcatcatca tcgccgcgat actgttcgcg
      301 gtcgtcgtct acttcgtgcg tcatcatcgt gtgcgcgtcg cgctgatgct gccgctgttt
      361 ttcgcttcgc tgctcgagga tttcggtttt tatacgctgg cgccgttgtt catcatcggc
      421 gtcatgtacg tgttcggcga gcgccgcccg aagcacatgg ccgtaatcac tgcagtgctg
      481 atgggattcc tgctgctgct gttcgtcaag atcctctacg tcggcctgcc ggtcggcaat
      541 attcgcccgt tctacgactt cggcagctgg gtcgtggaaa tgctgcaata aggaccggcg
      601 atggaaacct tcaataattt cctgcagggc tcgatcacgc tgttcagcga cccgatcaac
      661 atcctgatct tcctcggcgg cgtcgtcggc ggcatgctgt tcggcgcgat ccccggggtc
      721 agcatgctga cgctggcggc gatcctgctg ccgtttaccg cgcacctgtc ggccgagcag
      781 ggcatcatgc tgttttcggt gatctactgc accggcgtct acggcggtgc gatcaccgcg
      841 atcctgttca atattccggg cgccccggaa aacgcgccga ccgcgttcga cggctatccg
      901 atgaccaaga acggccaggc cggcaaggcg gtcggcgccg cggtcatgtg ctcggcgctc
      961 ggcggcgtgg tatcgacgat catcatgatg gcggccaccg caccgatcgc cgactgggcg
     1021 atctccgcct tcggcccgcc ggaaatcttc gcgctggtat ttttcggtct cgcggtggcg
     1081 gccacggtcg gcgccaagac cttctggaag ggctggctgt cgatcctcat cgggctgatg
     1141 gtgtcggtgg tcggtctcga cccggtcggt ggcatcgagc gttacgattt cggcatgccc
     1201 tacctgctcg ccggcattca cttcattccg acgatactgg gctttttcgc ggtctcggag
     1261 atcttcgtgc aggccgagaa gaaggtgcgc ggctcctacg aagcaccgaa gctctcggtt
     1321 aactttccga cgctcgcgga actggtcgcg cacaaggtcg ccgtagtacg ctcgatcgtg
     1381 atcggctttt tctgcggcat tctgcccggc atcggcgcga cgctggccgc gttcatgggt
     1441 tacaacgagg cggtgcgctg gtcgaagacg ccggaggaat tcggcaaggg caagctcgag
     1501 ggcgtgattt cttccgagac cgcaaacaat gccgccaccg gcgcagcgat gattccgctg
     1561 ctggcgctcg gcctgcccgg cggcgcgctg accgcgatga tggtcggcgt cttccagatg
     1621 cacgacatgc agcccggtcc gctggtgttc ctgaccagcg ccgacctggt ctgggtggtg
     1681 ttcgcggcga tgttcttcgc caattgctcg atcctgctga tcggttttct cgaaaccaag
     1741 accatcatca acctgctgcg cattccgttc cagttcctgg cgccgctgat attgctgctg
     1801 gccacggtcg gcgcctacgc ggtgcgcggc ctgacgatcg acatcgtggt catgtttctc
     1861 gccggcgtga tcgggttctt cctgcgccgc tcgggctatt cgatcgccgg catcgtgctc
     1921 ggcgtgatcc tcggcagcat cggcgaacag accttcgcgc aggcgatgca gatgctgcac
     1981 ttcgagtgga gcggcttcct cgatcggccg atcgccgccg gcctgctcgc cgtcggcttc
     2041 atcaccctcg gcgccagcat ctacggcgaa ttcttcaaga aatccgcgcc aacttccaat
     2101 ggccccgatt cccccgcgga atcacatcgc tccggcgagg gctgatctcg agtctatttc
     2161 cgaaaaggaa acgagggata gttttcgggc ctctgctcgg ccaagaagcg ggtaactatt
     2221 gtcgcttggc gctgaaccat atcccctcgg tccgattttt tcttgctctc catagaattg
     2281 cacaatgttt cctgttgtat ttaaatgatg cccatggatt acctcgactt cgcgtccgac
     2341 ctgcctgtta acgggaggat tattttctcc tcggctgagg agagcgaaga ggtgatgcga
     2401 caatatggcg tcgaccagca actacggcaa tccagcaagg gaagttttca ggctgattgc
     2461 accatgctcg agaccgaaga agcaatacta tctgccgacc gttttgcaca agcttgcctg
     2521 gcgcatttcg agcctcctcc ggacaccgtc tctattctgg ccttcagatg tgccggtgga
     2581 gaattcctgg tgtcgggcga gaaagtaacc gaagacaacc tggttgtggt gcctcatgga
     2641 cacggtacag acctggtgtt ttcaaatctg gctggctccg aaggagttgc tatccccaaa
     2701 accaaatatt ctgaattgac agaagcgctt tgcccgtcat gcgcgcaacc ggaaggactg
     2761 cagatagcga aaggtaatcc ccgcctgctg caaggaatac gcaactcagt tatccggatt
     2821 atcgctgccg cgcaaccacg cgatgaaaat atagatgatc tggtcgcgcg catggttcta
     2881 acggctaacg ctgacccctg ggctgagcgc cctgagattt tttaccgaaa gcaagccaaa
     2941 atcgacattg cgaaacaggt gcagacattt atcgacgaaa actatcagga gcagatccgt
     3001 atcgaagatc tttgtcgagc taccgagcgg ggagtcagga cactg
//
LOCUS       DATCZV010000536         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_360735, whole genome shotgun sequence.
ACCESSION   DATCZV010000536 DATCZV010000000
VERSION     DATCZV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360735"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..263)
                     /gene="ffs"
                     /locus_tag="VIW27_12120"
     ncRNA           complement(168..263)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIW27_12120"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            395..868
                     /gene="smpB"
                     /locus_tag="VIW27_12125"
     CDS             395..868
                     /gene="smpB"
                     /locus_tag="VIW27_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY5740464.1"
                     /translation="MATKKNQSRDNTIAVNRQARHDYFIEDEIETGMALEGWEVKSLR
                     NGSLQLRESYVMVKNGEIWLIGAHISPLASASTHVKPDPTRSRKLLAHRGEIDRLVGL
                     VDRKGYTLVALSAYWVRGRAKLKLGIARGKKAHDKRASVKERDWKRDQARIMKSS"
     gene            complement(896..>2241)
                     /locus_tag="VIW27_12130"
     CDS             complement(896..>2241)
                     /locus_tag="VIW27_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5740465.1"
                     /translation="IFATPPLVRLTILGLKNLSPEVAEAGLMSGCNNWQLMIRVLIPT
                     ARRDILLGVNQVIMSCLAMVVIASLIGAKGLGNDLLIALNQLRIGLALEIGVCIVLIA
                     IVLDRLSLAWADKQIDYFADLSFTQRHRYSLIFAAVFVAGVLLAWIGSFFFREGFNYL
                     YLIPHNKGITTAPVWQAAVDWVWDTFFYTLKGFNEWLIVEVLLPTKNAYLGMPVAATF
                     LLLMGAGYIVGGFRVALAVGGFLLFIALTEWWDRALITAYLVTLSVLISTFLGIVAGT
                     LCAQHPVSTRIMLTICDTFQTFPSFIYLIPVMMLFGVTDTSVLIAIVVYATVPATRYT
                     IQGLLSVPPELQEAGSMSGVNRIQRYLFIELPMAFPHLALGVNQTVVFALSMVIIGAL
                     IGTDDLGQLILKSLSDRAGVGNGLVLGLCVAFIGLAVDQILRTWANQRKRQLGLA"
BASE COUNT          530 a          681 c          685 g          345 t
ORIGIN      
        1 cgcgtggtgc aggcggttgg catcgagcgc gttgaccagc gcctgcagca cgtgctcctg
       61 accgaccatt tgccgaaaat tggctggccg ccatttacgc gctaggacct ggtaactcat
      121 gcgaattcag cgaagatgga attatcaacc ggaaaaatgg cggcgacctg tatcagcaca
      181 cccccgcacc cgatgtccct gctaccgttg ctcccttccg ggcctggcgg gatttgcaga
      241 taatcgtcgc gaagggaccg aaacaggtca ccattgcaag tcgacgagtg tatagcgaag
      301 gccggtcggg tttcaaggcg cggcgagcga ttcacttgtc agcggcggcg attcacccga
      361 gaatatggcg ctcaagagcc tgccgagtcg attgatggcc actaaaaaga accagtccag
      421 ggacaatacc atcgccgtca accgccaggc acggcacgat tatttcatcg aagacgaaat
      481 cgagaccggc atggcgctcg aaggctggga agtcaagagc ctgcgcaacg gcagcctgca
      541 gctcagggaa agttacgtga tggtcaagaa tggcgaaatc tggctgatcg gcgcacatat
      601 cagcccgctg gcgagcgcct ccacgcacgt caagccggat ccgacccgca gccgcaagct
      661 gctcgcgcac cgcggcgaaa tcgatcgcct ggtcggcctg gtcgaccgca agggctacac
      721 gctggtggct ctgtcggctt actgggttcg cggccgtgcc aagctgaaac tcggcatcgc
      781 gcgcggcaaa aaggcacacg acaagcgcgc cagcgtcaag gaacgcgact ggaagcgcga
      841 ccaggcgcga atcatgaaat catcctgacc gctgccggcg cccggcttcc agaccctagg
      901 cgaggccgag ctgccgcttg cgctgattgg cccaggtgcg caggatctgg tcgacggcga
      961 gaccgataaa ggcaacgcac aggcccagca ccaggccgtt gccgacaccg gcacggtccg
     1021 acagtgactt caagatcagc tgacccaggt cgtcggtgcc gatcaacgca ccgatgatca
     1081 ccatcgacag cgcgaacacc acggtctgat tgacgccgag cgcgaggtgc ggaaaagcca
     1141 tcggcaactc gatgaacagg tagcgctgta tgcgattgac gccggacatc gagccggcct
     1201 cctgcagctc gggcggcacg ctcagcagcc cctgtatcgt gtagcgcgtg gccggtacgg
     1261 tggcgtagac gacgatcgcg atcagcaccg aggtgtcggt gaccccgaac agcatcatca
     1321 ccgggatcag gtagatgaac gacgggaagg tctgaaaggt gtcgcagatg gtcagcatga
     1381 tgcgcgtcga caccgggtgc tgcgcgcaca gggtgccggc gacgataccg aggaaagtcg
     1441 agatcagcac cgacaaggtc accagatagg cggtgatcaa cgctcgatcc caccactcgg
     1501 tcagggcgat gaacagcagg aagccgccaa ccgcgagagc gactcgaaaa ccgccgacga
     1561 tgtacccggc gcccatcaac agcaggaagg tggcggccac cggcatgccg agataggcat
     1621 tcttggtcgg caacagcacc tcgacgatca gccactcgtt gaaacccttg agcgtgtaga
     1681 agaaggtatc ccagacccag tcgacggcgg cctgccacac cggcgcggta gtgatgccct
     1741 tgttgtgcgg aatcaggtac agatagttga aaccctcacg aaagaaaaac gaaccgatcc
     1801 acgcgagcag cacgccggcg acgaaaaccg cagcgaaaat cagcgaatac ctgtgccgct
     1861 gggtaaacga cagatcggcg aagtaatcga tttgcttgtc ggcccaggcg agcgacagcc
     1921 ggtcgagcac gatcgcgatc agtacgatac agacaccgat ctcgagggcg agcccgatgc
     1981 gtaactggtt caatgctatc aacagatcat tgccgagccc cttggcgccg atcagcgaag
     2041 cgatgacgac catcgccagg cacgacatga tcacctggtt gacgccaagc agaatatccc
     2101 ggcgcgcggt cggaatcagc acccggatca tcagttgcca gttgttgcaa ccgctcatca
     2161 ggccggcctc cgccacttcg ggcgacagat ttttcagacc gagtatggtc aggcgcacca
     2221 acggcggagt cgcgaaaatt a
//
LOCUS       DATCZV010000537         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_574794, whole genome shotgun sequence.
ACCESSION   DATCZV010000537 DATCZV010000000
VERSION     DATCZV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574794"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VIW27_12135"
     CDS             complement(<1..128)
                     /locus_tag="VIW27_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740466.1"
                     /translation="MSQTDATTTKHTPMMQQYLRLKADYPDTLLFYRMGDFYELFY"
     gene            complement(372..1775)
                     /gene="gorA"
                     /locus_tag="VIW27_12140"
     CDS             complement(372..1775)
                     /gene="gorA"
                     /locus_tag="VIW27_12140"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY5740467.1"
                     /translation="MNSNCYDLIVIGGGSGGLAVAETAAQLGKSVALVETGKIGGTCV
                     NNGCVPKKIMWYAANLAHAADDARDFGIDAWRGKTDWKRLVRERDNYVANITSYWDGY
                     VNDNGIDRIDGRGRFVDASTVAVGEALYSAPHIVIATGSEPIMPPLPGAELGISSDGF
                     FALTEQPSRVAIVGGGYIGVELSGVLRALGSEVSLLAMEDRVLAQFDPLIGDMLAGEM
                     RRQGIDLKTGCQVGALRRDARGIQVSAVDGDSLGHFNSVIWAVGRRANSAGLDLAAAG
                     LQASAGGYIAVDEYQNTAIAGIYAIGDVTGQASLTPVAIAAGRKLAARLFGGQPRARV
                     DYRDIPSVVFAHPPVAALGLAEAKARQRYGDDAVTVYQTEFTPMRHALSADGARTAIK
                     LVCVGEQQRIAGIHMVGDGADEILQGFAVAVKMGATKADFDSTIAIHPTSAEELVTLK
                     QPRDALLEVTEEWREAS"
     gene            complement(1851..2594)
                     /locus_tag="VIW27_12145"
     CDS             complement(1851..2594)
                     /locus_tag="VIW27_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006632162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY5740468.1"
                     /translation="MSNNHEGKAVPQVTFKTRRDHQWVELTSDDIFKGKTVVVFSLPG
                     AFTPTCSSTHVPRYNQLAPAFRKHGVDDIVCISVNDAFVMNEWQREQHADKLTFLPDG
                     NGHFSAGMDMLVDKEDLGFGKRSWRYSMLVKDGIVEKMFVEPDLPGDPFEVSDADTML
                     DYIAPGAAKPRDVTIFTREGCEFCARAKGMLHELEIGYEELVLNRDYTEATLRAVCGR
                     SSVPQVFVNGEHIGGSEELDRHLARDIAA"
     gene            complement(2649..2849)
                     /locus_tag="VIW27_12150"
     CDS             complement(2649..2849)
                     /locus_tag="VIW27_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEY5740469.1"
                     /translation="MSKTLTFAIVHFTVAFSVAYLVSGSWIVGGAVALIEPAVNTLAY
                     HFHEKAWERVRGNRRDPTAMAA"
     gene            complement(3027..>3461)
                     /locus_tag="VIW27_12155"
     CDS             complement(3027..>3461)
                     /locus_tag="VIW27_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5740470.1"
                     /translation="PFWIAYPRDHPLYLQETITRQDLDELDLLLLSEGHCLAQQVMEL
                     CHVSQRANSGEMADLRASSLETLMQLVGAGFGSTLVPALAMRGSWASGSGVVTRELDF
                     DEAHRRVSLFHRKSFPRIAALQALIAVIMDNLPNTVQPLYRV"
BASE COUNT          586 a         1062 c         1151 g          662 t
ORIGIN      
        1 tcgtagaaaa gctcgtagaa atcgcccatg cgatagaaca gcagcgtgtc gggataatcc
       61 gccttcagac gcaggtattg ctgcatcatc ggcgtatgct tggtcgtcgt ggcgtctgtc
      121 tggctcatgg ggttccgctg cgcccgggcg gttacggctg cgggcaggtc gtggccaatt
      181 gtactcgact tggcggcttg cgcccaaggt gtacgccgtg gcctcagttc atggctcagg
      241 cggcggcgcc ggcagtcaac cagcaacgga agccaccgcc ggatggccgg cccggggtta
      301 cggccgaggt ctaatggcag gtgcggcgcg gtgcagccgg gccgcctcgg cgccgcccgg
      361 ccggggtatg tctagctggc ctcgcgccat tcctcggtga cctcgagcag cgcgtctcgc
      421 ggctgtttga gggtcaccag ttcttcggcg ctggtcgggt gaattgcgat ggtgctgtcg
      481 aaatcggcct tggtggcgcc catttttacc gctaccgcga agccctgcag gatttcatcg
      541 gcgccgtcgc cgaccatgtg aatgccggcg atgcgttgtt gctcgccgac gcacaccagc
      601 ttgatcgcgg tgcgcgcgcc atccgcggac aacgcgtggc gcatcggcgt aaactcggtc
      661 tggtaaacgg taaccgcgtc gtcgccgtaa cgctgacgcg ccttggcctc ggccagcccc
      721 agcgccgcca ccggcgggtg tgcgaatacc acgctcggaa tgtcgcggta gtcgacccgc
      781 gcccgcggct ggccgccgaa cagccgcgct gccagtttgc ggccggcggc gatggctacc
      841 ggggtcagcg aggcctggcc ggtgacgtcg ccgatcgcat agatgcccgc gatggcggtg
      901 ttctggtact cgtcgacggc gatgtagccg ccggccgagg cctgcaggcc ggctgccgcg
      961 agatcgagcc cggccgaatt cgcgcgccgg cccacagccc agatcacgct gttgaagtgg
     1021 cccagcgagt cgccgtcgac ggcgctgacc tggatgccgc gagcgtcgcg ccgcagcgcg
     1081 ccgacctggc aaccggtctt gaggtcgatc ccctggcggc gcatctcgcc ggcgagcatg
     1141 tcgccgatca gcgggtcgaa ctgcgccaac acgcggtcct ccatggccag caggctgacc
     1201 tcggaaccga gcgcccgcag cactccggac aattctacgc cgatgtaacc gccgccgacg
     1261 atggcgaccc gcgacggctg ctcggtcagc gcgaagaaac cgtccgagga gatgccgagc
     1321 tcggcacccg gcagcggcgg catgatcggc tcgctgccgg tggcgatgac gatgtgcggc
     1381 gcgctgtaca gcgcttcgcc gaccgcgacc gttgaggcgt cgacaaagcg gccgcgacca
     1441 tcgatgcggt cgatgccgtt gtcgtttacg tacccgtccc agtaagacgt gatgttggcg
     1501 acgtagttgt cgcgttcgcg gaccaggcgt ttccagtcgg tcttgccgcg ccaggcatcg
     1561 atgccgaagt cgcgggcgtc gtcggccgcg tgcgcgaggt tggcggcgta ccacatgatt
     1621 ttcttcggca cgcagccgtt gttgacgcag gtgccgccga tcttgccagt ttcgaccagt
     1681 gccaccgatt tgcccagttg cgcggcagtt tcggccaccg ccaggccgcc gctaccgccg
     1741 ccgatgacga tcaggtcata gcagttgctg ttcatgaata aatctcccgc tagtatcaag
     1801 acgggcgacc cgctaagggc cgcccgtgct gcttgcgatg ggtcaccgaa tcaggcggcg
     1861 atatccctgg cgaggtggcg gtcgagctct tccgagccgc cgatgtgctc gccgttgacg
     1921 aatacctgcg gtacgctgga gcggccgcag acggcgcgca gcgttgcctc ggtgtagtcg
     1981 cggttcagca ccagttcctc gtagccgatc tcgagctcgt gcagcatgcc cttggcgcgc
     2041 gcgcagaatt cgcagccctc gcgggtgaag atggtcacgt cgcggggttt ggcggcaccc
     2101 ggcgcgatgt agtcgagcat ggtatccgcg tctgagacct cgaagggatc gcccggcaga
     2161 tcgggctcga caaacatttt ttcgacaatg ccgtctttca ccagcatcga gtagcgccag
     2221 gagcgcttgc cgaagccgag gtcttccttg tcgaccagca tgtccatgcc ggcgctgaag
     2281 tggccgttgc cgtcgggcag gaaggtcagc ttgtcggcgt gctgctcgcg ctgccactcg
     2341 ttcatgacga aagcgtcgtt gaccgagatg cagacgatgt cgtcgacacc gtgcttgcgg
     2401 aatgcgggtg ccagctggtt gtagcgcggc acgtgtgtcg acgagcaggt cggcgtgaac
     2461 gcacccggca gcgagaagac gaccacggtc ttgcccttga agatgtcgtc gctggtcagc
     2521 tcgacccatt ggtggtcgcg acgggttttg aaagtgacct ggggtactgc tttaccttcg
     2581 tggttgtttg acatgatttt tgctcctgtc tactagttgc taaaaagtta aaaaaatcgg
     2641 ttgctgcttc aggcggccat cgcggtcggg tcgcggcggt tgccgcgcac ccgttcccag
     2701 gccttttcgt gaaagtgata ggcaagcgta ttgactgccg gctcaatcag tgccaccgcg
     2761 ccgccgacaa tccagctgcc cgagaccagg taagcaaccg agaacgcgac cgtgaagtga
     2821 acgattgcga aagtcagggt tttgctcatc ggaatctcgt cttcagcccg aggctgtgtt
     2881 tgaacacggg tgaaataatg ggggcaaacg aagaatagtt aaaatcgttt ataacgattt
     2941 aaacgatagg ttttgacgat taatagtttt tgggcctatt cgcaccgacc ttacagggcc
     3001 ggattgctgt cgaatgatgt cgcgcgctag acccggtaaa gcggctgcac ggtgttgggc
     3061 aggttatcca tgatgaccgc gatcagcgcc tgcagcgccg cgatgcgcgg aaagcttttg
     3121 cggtggaaca gcgatacgcg gcgatgcgcc tcgtcgaagt cgagttcgcg ggtgaccacg
     3181 ccgctgccgc tggcccagct accgcgcatc gccagcgccg gcaccagcgt cgaaccgaag
     3241 ccggcgccga ccagctgcat cagggtttcc aggctcgacg cgcgcaggtc ggccatctcg
     3301 ccgctgtttg cacgctggct gacatggcaa agctccatga cctgctgtgc gaggcaatgg
     3361 ccttcggaca gcaatagcaa atccagttcg tcgaggtcct ggcgcgtgat ggtttcctgc
     3421 aggtaaagcg gatggtcgcg gggataggcg atccagaagg g
//
LOCUS       DATCZV010000538         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_44365, whole genome shotgun sequence.
ACCESSION   DATCZV010000538 DATCZV010000000
VERSION     DATCZV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44365"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VIW27_12160"
     CDS             complement(<1..941)
                     /locus_tag="VIW27_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018144355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEY5740471.1"
                     /translation="MSSRKTYDAGVKEYRETYWMPEYTPADTDILACFKITPQPGVPR
                     EEAAAAVAAESSTGTWTTVWTDLLTDLDYYKGRAYSIEDVPGDDSCFYAFIAYPIDLF
                     EEGSVVNVFTSLVGNVFGFKAVRGLRLEDVRFPLAYVKTCNGPPNGIQVERDRLNKYG
                     RPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSQPFMRWNHRFDFCMEA
                     IHKAEAETGERKGHYLNVTAPTPEEMYKRAEYAKELGAPIIMHDYLTGGFCANTGLAQ
                     WCRDNGMLLHIHRAMHAVLDRNPIHGIHFRVLAKALRL"
     gene            complement(964..2046)
                     /gene="fba"
                     /locus_tag="VIW27_12165"
     CDS             complement(964..2046)
                     /gene="fba"
                     /locus_tag="VIW27_12165"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174130.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY5740472.1"
                     /translation="MARITLRQLLDHAAERGYGVPAFNINNMEQVLAIMNAASAVDAP
                     VILQASRGARGYAGDIMIRKMVEAAEEMYPQIPICLHQDHGNNRATCVSAMQASFTSV
                     MMDGSLEADAATPASYEYNVDITAEVTRIAHDIGVSVEGELGCLGSLETGEGEKEDGH
                     GFEGSLSMDQLLTDPEQAVDFVTRTRVDALAIAMGTSHGAYKFSRKPDGEILAMNVVK
                     EINAKLPEVHLVMHGSSSVPQELQDIFNQFGGEMPQTYGVPVEEIVRGIKHGVRKVNI
                     DTDCRLAMTGEFRRIAEQDKSQFDPRKFLTPALKAMEDLCRDRFEQFETAGNASKITP
                     ISVADMAKSYASGALDPKVASSKTAA"
BASE COUNT          394 a          667 c          668 g          470 t
ORIGIN      
        1 aggcgcaacg ccttggccag tacgcggaag tgaatgccgt gtattggatt gcgatccagc
       61 accgcgtgca tcgcgcggtg gatgtgcagc agcatgccgt tatcgcggca ccattgcgcg
      121 agaccggtgt tggcacagaa accgccggtc aggtagtcgt gcatgatgat cggcgcgccc
      181 agctccttgg cgtattcggc gcgcttgtac atttcttcgg gcgtcggcgc ggtaacgttc
      241 aggtagtgac ccttacgctc gccggtttcc gcctcggcct tgtgaatcgc ctccatgcag
      301 aaatcgaaac ggtgattcca gcgcatgaac ggctgcgaat tgatgttttc gtcgtccttg
      361 gtgaagtcga gcccgccgcg caggcactcg taaaccgcgc gcccgtagtt cttcgccgac
      421 aggccgagct tgggcttgat ggtgcagccc agcagcgggc ggccgtactt gttcaagcgg
      481 tcgcgctcga cctggatgcc gttcggtggc ccgttacagg tcttgacgta ggccagcggg
      541 aagcgcacgt cttcgagacg caagccgcgt accgccttga agccgaacac gttgccgacc
      601 agcgaggtga acacgttgac caccgaacct tcttcgaaca ggtcgatcgg ataggcaatg
      661 aacgcgtaga aacagctgtc atcgccgggt acgtcctcta tcgaataggc gcgccccttg
      721 taatagtcga gatcggtcaa caggtcggtc cagaccgtgg tccaggtacc ggtggacgat
      781 tccgcggcaa ccgcggcggc ggcttcctcg cgcggcacgc cgggctgcgg cgtgatcttg
      841 aaacaggcga ggatatcggt gtccgccggc gtgtattccg gcatccagta ggtttcgcgg
      901 tattctttta cgcccgcgtc gtaggttttt ctggaactca tttttcgttc tccaaataaa
      961 aaatcaggca gccgtcttgc tgctggcaac tttaggatcg agcgcgccgc tggcgtatga
     1021 cttcgccatg tcggcaacgc tgatcggggt aatcttcgag gcattgcccg cggtttcgaa
     1081 ctgttcgaaa cggtcgcggc acaggtcttc catcgccttc aacgccgggg tcaggaactt
     1141 gcgcgggtcg aactggctct tgtcctgctc ggcgatacgg cgaaactcgc cggtcatggc
     1201 gaggcgacaa tcggtatcga tgttgacctt gcgcacgcca tgcttgatgc cgcgcacgat
     1261 ttcctcgacc gggaccccgt aggtctgcgg catttcaccg ccgaactggt tgaagatgtc
     1321 ctgcaactcc tgcggcaccg atgaagagcc gtgcatcacg aggtgcactt cgggcagctt
     1381 tgcattgatt tccttgacca cgttcatcgc caggatttcg ccatccggct tgcggctgaa
     1441 tttgtaggcg ccatgcgagg tacccatcgc aatcgcgagc gcatcgaccc tggtacgggt
     1501 cacgaagtcg accgcctgct ccggatcggt cagcagctga tccatgctca ggctgccctc
     1561 gaaaccgtgg ccatcctctt tctcgccctc gccggtttcg agcgagccga ggcagccgag
     1621 ctcaccctcg accgagacgc cgatgtcgtg cgcgatgcgc gtgacttcgg cggtaatgtc
     1681 gacgttgtat tcgtaactcg ccggggtcgc ggcatcggcc tcgagcgaac cgtccatcat
     1741 caccgaggtg aaactggcct gcatcgccga cacgcaggtg gcgcggttgt tgccgtgatc
     1801 ctggtgcagg caaatcggta tctgcggata catttcctcg gcggcctcga ccatcttgcg
     1861 gatcatgatg tcgccggcgt aaccgcgcgc accgcggctg gcctgcagga ttaccggcgc
     1921 atcgaccgcg ctcgccgcat tcatgatcgc gagcacctgc tccatgttgt tgatattgaa
     1981 cgcgggtacg ccgtagccgc gctccgcggc atgatcgagt aactgtctaa gcgtaattct
     2041 tgccatgtcg aaatctctcc aggttaaatc tgtaatagac ggcgcacttc gtcatgcacc
     2101 gcctgcgcgg taaggccgaa atgctggtat aaatcctcgg cgggcgccga ggcgccgaaa
     2161 ccgctgatgc cgatgatcga actgcgcttg ccgagccat
//
LOCUS       DATCZV010000539         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_466816, whole genome shotgun sequence.
ACCESSION   DATCZV010000539 DATCZV010000000
VERSION     DATCZV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466816"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..299)
                     /locus_tag="VIW27_12170"
     CDS             complement(81..299)
                     /locus_tag="VIW27_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740473.1"
                     /translation="MSTKIVSQIVNCSTRQRLVEVTCMVSGGWFNRRYNVISCPAMYD
                     AGPGCDLRCKAQLGNRSLTNQAVFGLRS"
     gene            complement(406..1518)
                     /locus_tag="VIW27_12175"
     CDS             complement(406..1518)
                     /locus_tag="VIW27_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019294418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5740474.1"
                     /translation="MKIERISVWQLELPLHKPYWLSGGRLRFDSLDSTLLRIDTDAGI
                     FGWGEGCPWGVTYLPAFGKGIRAGIDELAPLLLGRDPRQLDAINRAMDIALPGHPYVK
                     SALDIACHDIAARSAGQSLCEFLGAREPEPVPIASSVSTAAPRAMLDEVERFRDRGYR
                     VHSCKVGADVHVDIARIDLLAANERDGEIIFYDVNRAWLPREAITVMNSVSGLTSWFE
                     QPCETLDEIAQVRRHTNFPIGIDEGLHGFDDLLRIQRDGIAEVVNIKINRVGGLTKAR
                     RLRDFCLATGITMLIMDTGGTVLADTAVAHFAQAIPRPSCLGVWCCQEMVSIDPAPGQ
                     GARNVDGCFTAPGLPGIGVEPEPGLLGEPLAVYSLA"
     gene            1629..>2094
                     /locus_tag="VIW27_12180"
     CDS             1629..>2094
                     /locus_tag="VIW27_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY5740475.1"
                     /translation="MYVPEKFRIAQTDEIFAFLAANAFGQLVSQHDGRPMVSHLPFLV
                     AADRGTLKCHVARQNPQWQQLEVQQVLATFLGPHDYISPSWYRDAGVPTWNYQAVHVY
                     GRCRVFEQADELTALVDALSGVYESRFDDPWQPRYPDSMLKAIVGIELEIDDI"
BASE COUNT          406 a          666 c          654 g          368 t
ORIGIN      
        1 gcagcgacta tcggcagaag gccggctgtg ctcgactccg tcgttggcgc ttgcttgcag
       61 ctggggtccg cggggcgcgg ctaggaacgt aggccgaaga ccgcttggtt ggtcaacgac
      121 cggtttccca gctgcgcctt gcagcgcaga tcacaacccg ggccggcgtc gtacatggcg
      181 ggacacgaga tcacgttgta gcgccggttg aaccagcctc cagagaccat gcaggtaacc
      241 tccaccagcc gctggcgagt cgagcaattg acaatctggg acactatctt ggtactcaca
      301 aaagtctcct ggctttgctg aaacccgcag tttgcctgca atagcagacg aaatattgac
      361 cgcgatcaag cggaccgatc tgttttcggc ggcgggcaac cggaatcagg ccaacgaata
      421 gacggcaagc ggctcgccga gcaggccggg ttccggctcg acgccgatgc cgggcagccc
      481 gggggcggtg aaacagccgt cgacattgcg cgcgccctgc cccggcgccg ggtcgatgct
      541 gaccatttcc tggcagcacc agacacccag acagctgggc cgcggaatcg cctgcgcgaa
      601 gtgcgcgacc gcggtatccg cgagtaccgt gccgccggtg tccatgatca gcatcgtaat
      661 gccggtggcc aggcagaaat cgcgcaagcg ccgcgccttg gtcaggccgc cgacgcggtt
      721 gatcttgata ttgacgacct cggcaatgcc gtcgcgctga atccgcagca ggtcgtcgaa
      781 tccgtgcaga ccttcgtcga taccgatcgg gaagttggta tgacggcgca cctgggcgat
      841 ctcgtcgagc gtctcgcagg gttgctcgaa ccacgaggtc aggccgctaa ccgagttcat
      901 caccgtgatc gcctcgcgtg gcagccaggc ccgattgacg tcatagaaaa taatctcgcc
      961 gtcgcgctcg ttagcggcca gcaggtcgat gcgcgcgata tcgacatgca cgtcggcgcc
     1021 aaccttgcac gagtgcaccc ggtaaccgcg atcgcgaaag cgctcgacct cgtcgagcat
     1081 cgctcgcggc gcggcggtcg acacgctgga cgcgatcggc accggctcgg gctcgcgcgc
     1141 gccgaggaat tcgcacaggg actggccggc cgatcgcgcg gcaatgtcat ggcaggcgat
     1201 atccagtgcc gacttgacgt aaggatgtcc cggcagcgcg atatccatgg cgcgattgat
     1261 ggcgtcgagc tggcgcggat cgcggcccag cagcaacggc gcgagttcgt cgatgccggc
     1321 gcggatgccc ttgccgaagg ccggcaggta agtcacaccc caagggcagc cctcgcccca
     1381 gccaaagatg cccgcgtcgg tatcgatgcg cagcagggtc gagtcgaggc tgtcgaatcg
     1441 aagtcgaccg ccggacagcc agtagggttt gtgcaatggc agctcgagtt gccagacgct
     1501 gatgcgttcg atcttcatgc gctcaatcta ctctcgtgat ctgcgggcgt aaaacaaata
     1561 gaatcgatgt ccgtaatcgc taccgtctat caactcgcgg ctcaccgagg ccgcaaactg
     1621 gagttgccat gtacgtcccc gagaaattca ggatcgccca aacggacgaa attttcgcct
     1681 tcctcgcggc caatgctttc ggccagctgg tttcgcaaca cgacgggcgg ccgatggttt
     1741 cccacctgcc gtttctggtc gccgcagacc gcggcaccct gaagtgccat gtcgctcggc
     1801 aaaatccgca gtggcagcaa ctcgaagtcc agcaggtgct ggcgaccttc ctcgggcctc
     1861 acgactacat ctcgccgagc tggtaccggg acgccggggt gccgacctgg aactaccagg
     1921 cggtgcacgt ctacggccgc tgccgcgtgt tcgaacaagc cgacgaactg acggcactgg
     1981 tcgatgcatt gtccggcgtt tatgaatcgc gcttcgacga tccctggcaa ccgcgttatc
     2041 ccgattcgat gctaaaagcc atcgtcggta tcgagctcga aatcgacgac atcc
//
LOCUS       DATCZV010000540         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_149352, whole genome shotgun sequence.
ACCESSION   DATCZV010000540 DATCZV010000000
VERSION     DATCZV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149352"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VIW27_12185"
     CDS             <1..1681
                     /locus_tag="VIW27_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY5740476.1"
                     /translation="DAHGEVAIYFEAAAVIVTLVLLGQVMELRARNRTGAAIRALLGL
                     APKTALRLNADGSESEVPLEQVMVGDRLRVRPGEKVPVDGTVVEGNSSVDESMLTGEP
                     IPVEKIAGEKVIGATINGTGSLVIEADRVGADTMLSQIVQMVANAQRSRAPIQKLADV
                     VAGYFVPAVVVVAIATFIVWAWIGPEPAMAFAIINAVAVLIIACPCALGLATPMSIMT
                     ATGKGASMGVLFKDAEAIEVMRKVDTLVVDKTGTLTEGKPRLVGIGAAGGMDESALLR
                     IAATLERGSEHPLAAAIVAGAKERDIELGKVENFASVTGKGVTGEIDGKLVALGNAGL
                     LKSLDVDPGAFAQRAEAQRAEGQTVMYIAVDGVMVGTIGVADPIKETTPHAIEALHAE
                     HVRIVMLTGDSETTARAVAEKLGLDEVIAGVLPDQKAETVKRLQSEGHIVGMAGDGIN
                     DAPALAQAEVGIAMGTGTDVAMESAGVTLVKGDLRGLVRARRLSRATMANIRENLFFA
                     FIYNSLGVPIAAGVLYPWLGVLLSPMIAAAAMSFSSVSVIANALRLNRVRL"
     gene            1851..2417
                     /locus_tag="VIW27_12190"
     CDS             1851..2417
                     /locus_tag="VIW27_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740477.1"
                     /translation="MKVIFRLLPILVLILHSPNLIASSDSDELKAISGQVAALRQEVA
                     DLKNSVEMLMKLRPSVTMMMTEISERMHVMHYAGDAEDWAVASHELNVLKDLVSRIEL
                     VDPEKGAMAKGFLLGPFSQIEAAIEHGSQESFDEALDATVANCNSCHVAAGSPTMRVV
                     LDATDSLSMRHSHALGKSEKPGDHMHKH"
     gene            complement(2521..3321)
                     /locus_tag="VIW27_12195"
     CDS             complement(2521..3321)
                     /locus_tag="VIW27_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5740478.1"
                     /translation="MVRAVNDVSLDIGQGEMVLIMGPSGSGKTTLLSMLGALLSPSSG
                     QIIVDGEDISSMKAADLARLRARKIGFVFQSFNLLEALNVEENILLPAPLAPSGIDGA
                     RDRLEQMLGELDLAARRKALPRTLSGGEKQRVAIARALINRPQIILADEPTGNLDSHK
                     GQEVMMILHDVARDQKCAVLMVTHDPRVEEIADRILWLEDGRLRDRKQERHNWVIDPV
                     CGMRVDEWTATISTVYRQHKYVFCSARCLQRFKAQPERYAAMTNPVDS"
BASE COUNT          678 a         1009 c         1082 g          647 t
ORIGIN      
        1 cgatgcccac ggcgaggttg caatctattt cgaggccgcc gcggtcatcg ttacgctggt
       61 gctgctcggc caggtcatgg agttacgcgc acgtaatcga accggcgcag cgatcagggc
      121 gctgctcggc ctggccccca aaacagcgct gcggctcaac gccgacggca gcgaatccga
      181 ggtgccgctg gagcaggtca tggtcggcga ccggctgcgt gtgcgcccgg gcgaaaaggt
      241 cccggtcgat ggcaccgtcg tcgaaggcaa cagctcggtc gacgaatcga tgctgaccgg
      301 cgagccgata ccggtagaga agatcgccgg cgaaaaggtc atcggcgcga ccatcaacgg
      361 caccggcagc ctggttatcg aggcggatcg cgtcggtgcc gacaccatgc tgtcgcaaat
      421 cgtacaaatg gtcgccaacg cgcagcgcag ccgcgcgccc atccagaaac tcgccgacgt
      481 cgtcgccggt tacttcgtgc ccgcggtagt ggtggtcgcg atcgccacct tcatcgtttg
      541 ggcctggatc gggccggagc cggcgatggc cttcgcgatc atcaatgcgg tcgcggtgct
      601 gatcatcgcc tgcccctgcg cgcttggcct cgcgacgccg atgtcgatca tgaccgccac
      661 cggcaagggc gcgtcgatgg gcgtgctgtt caaggatgcc gaggctatcg aggtgatgcg
      721 caaggtcgat acgctggtgg tggacaagac cggcacgctg accgagggca agccgcggct
      781 ggtcggcatc ggagcggcgg gcggcatgga cgaatctgca ctgctgcgga ttgcggccac
      841 gctggagcgc ggcagcgagc acccgctggc ggccgcaatc gttgccggcg cgaaggagcg
      901 cgatatcgag ttgggtaagg tcgaaaattt cgcctcggtc acgggcaagg gcgtaaccgg
      961 agagatcgac ggcaagttgg tggcgctcgg taacgcgggg ctgctgaagt ccctcgatgt
     1021 cgatccgggc gcgttcgcgc agcgtgccga ggcgcaacgc gccgagggcc agacagtaat
     1081 gtatattgcc gtcgacggcg tcatggtagg tactatcggg gtcgccgacc cgatcaagga
     1141 aacgacgccg catgcgatcg aggcgctgca cgcagagcac gtgcgcatcg tcatgctgac
     1201 cggggacagc gaaaccactg ccagggcggt tgctgaaaag ctcggtctcg acgaagtcat
     1261 tgccggcgtg ctgcccgacc agaaggccga gaccgtcaag cggctgcagt ccgagggcca
     1321 catcgtcggc atggccggag atggcatcaa cgatgccccg gcgctggcgc aggccgaggt
     1381 cggaatcgcc atgggcaccg gcaccgacgt ggccatggaa agcgccgggg ttaccctggt
     1441 caagggcgat ttgcgcggcc tggtccgggc gcgccggttg agccgggcga ccatggccaa
     1501 catccgcgaa aacctgtttt tcgcgttcat ctacaatagc ctcggcgtgc ctatcgccgc
     1561 cggggtgctc tatccgtggc ttggtgttct gctcagcccg atgatcgcgg cggccgcgat
     1621 gagcttcagc tcggtctctg ttatcgccaa cgcgttacgc ctcaatcgtg tacgactttg
     1681 aatgcagtga acaggttaga catgcaacgg accaggtcag agcaaagccc atggcggggt
     1741 gagcgcagcc gtgttgtttt tactaagtgt atttacattg aggacctgcg gaatgaatcc
     1801 gacccgacga caacaagcaa gcttcgttaa cttagctaag gagtacctaa atgaaagtta
     1861 tatttcgatt attaccaata ctggtgttga tcctgcatag cccgaatctc attgcaagct
     1921 ccgattcaga tgagcttaag gccatatcag gccaggttgc cgctttgcgg caggaggttg
     1981 ccgacctgaa aaattcagtc gagatgctaa tgaaactgcg cccgtcggtg accatgatga
     2041 tgaccgaaat ttccgaacgc atgcacgtca tgcactatgc cggtgatgcc gaagactggg
     2101 cggtcgcatc tcacgaactg aatgtactca aagatctggt tagcaggatc gaactagtag
     2161 accccgagaa gggagccatg gcaaagggtt ttctgctggg gcccttcagt cagatcgaag
     2221 ccgcaatcga gcatggaagc caggaatctt tcgacgaggc tctcgatgcc acggtcgcca
     2281 attgtaacag ctgtcacgtg gcggccggca gcccgacgat gagggtggtg ctagatgcca
     2341 ccgacagcct gagcatgcgt cattcgcacg cgctcggaaa atcggagaaa cccggcgatc
     2401 acatgcacaa gcactgaatc gcggcggcgc ggcccaaaag cgcgaaatcg cctgcgcatg
     2461 cagggaggtc ctttggtatc gtcgatgtaa tcgggtaaca ggacgagcca cttcccgttt
     2521 tcacgaatcg accgggtttg tcatcgcggc gtaccgctcc ggctgcgctt tgaatcgctg
     2581 caggcagcgc gccgagcaaa agacgtattt gtgctgccgg tatacagtgg atatagtcgc
     2641 agtccactcg tcgacgcgca tgccgcagac cggatcgatg acccagttgt gccgctcctg
     2701 cttgcggtcg cgcagccggc cgtcttcgag ccataggatg cgatcggcga tctcctcgac
     2761 tcgcgggtcg tgagtcacca tcagcacggc gcatttctgg tcgcgggcca catcgtgcag
     2821 gatcatcata acttcctgcc ccttgtgtga atcgaggttg ccggtaggtt cgtccgcgag
     2881 aatgatctgc ggtcggttta tcagcgcgcg cgcaatcgcg acgcgctgct tttccccgcc
     2941 ggaaagcgtt cgcggcagcg ccttgcgccg cgccgcgaga tccagttcgc ccagcatctg
     3001 ctccaggcgg tcccgcgcgc cgtcgatgcc ggatggcgcg agtggcgccg gcagcagtat
     3061 attctcttcg acattaagcg cctccagcag gttgaatgac tggaacacga aaccgatttt
     3121 tctcgccctc aagcgcgcca ggtccgctgc cttcatcgag ctgatatcct cgccgtcgac
     3181 gatgatctgt ccactgctgg gactgagcaa ggcgccgagc atcgacagca gtgtcgtttt
     3241 cccggatccg gagggcccca tgatcaaaac catttcgccc tgcccgatgt ccaggctgac
     3301 atcgttgact gcgcgtacca ttgaagcacc agagccgaag cttttactga gatgactggc
     3361 ctgtagaatg gttgtatcgc tcatcgcaat tcctaactgt gaaagatact cacggg
//
LOCUS       DATCZV010000541         7587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_202928, whole genome shotgun sequence.
ACCESSION   DATCZV010000541 DATCZV010000000
VERSION     DATCZV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202928"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1698
                     /locus_tag="VIW27_12200"
     CDS             379..1698
                     /locus_tag="VIW27_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740479.1"
                     /translation="MRSVLFGENQFLRGRRSILPAAALIASSMLVTACTFDSVVTTDG
                     SQPVTEVKVGDQWDTPFDRLGVVPLLPPSEDVRAGDIFAYPFNPDLQIRSSNRSRRSE
                     ISISPRWDSLDLLQELEDEYRLRPDWPAVADSYPQSAGGPQPGESSEPAAAQARSLFS
                     PDQAPVRLRSFGVPELDTFTLSDAQANALVPSAAINLVFGSAWNDSKAISIRVNSAET
                     YSLGLRKVISAALDTSGPVQVLREPYRNQLSLIADPTADSVWLRILSDVVYVRSIDII
                     IQSRSAFDEDEVAMASEFVDEVEETVTVNAQEIAADEARATGEMQAAAEDGESSGKDP
                     KSENSPIDAEILRTETITEIAPDHALDPAYAAFVRADAINEKLIAVDADRSSASQLRF
                     VSVTDDSVTVRRVWQRGLAIGARGLSLEVDKLTGAVLRSATMGSLIP"
     gene            2173..2676
                     /locus_tag="VIW27_12205"
     CDS             2173..2676
                     /locus_tag="VIW27_12205"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY5740480.1"
                     /translation="MSSGYTLLEILIAVILVSLLMAFAVPAMRAFTQNDRMITNVNTL
                     IGHLNYARSEAVKRSQQVTVCASNDATTCSGTWSDGWIVYVDADADNSFTAGEEIIRV
                     KQSLEGGNSLTATDIGGQITYNNRGFVNDASVGSLQLCDDRSGAYGKTVRITSLGRVR
                     LEVDTSC"
     gene            2670..3146
                     /gene="pilV"
                     /locus_tag="VIW27_12210"
     CDS             2670..3146
                     /gene="pilV"
                     /locus_tag="VIW27_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY5740481.1"
                     /translation="MLTRSRQQRGMTLIEAMIALLVISVGLLGIASLQLTSMKQNVSA
                     LNHSRAVWYTYSMSDRIRANIDEFANYDGIDTSGEYAQDCMSQPCSTAQMRIADAADW
                     SAMVSNLPAGRGIIASNADGLMVSVMWDDEGTGATGTGCGPDANVDLTCYSLEVGQ"
     gene            3143..4141
                     /locus_tag="VIW27_12215"
     CDS             3143..4141
                     /locus_tag="VIW27_12215"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY5740482.1"
                     /translation="MNGVNRQRGISLVELLIAMVIGLFLLAGIYQVYISNKATFAFTN
                     AIAEVQENGRFALDLMSQDLRMASEWGCIQLDPKDTANINDTLSATTLPGYDTEFHDF
                     IGKSGIEGNNNTGLNNSDTLTIRGGRPSQANVEGPFFPPTSKQLVTDAVNSIGIGDIV
                     LVARCGANDLLIETEADIFRVNETFPFNNNTQRVLTFSANKSQQFENDAMVIVLQTVN
                     YFIAAGESGEPALFRREFGNAPRELIEGTEDLQILYGVDTDNDDFPNQYLTADKVLNF
                     NDVVAVRVMLLVRSVDDNVTDTPQTYTYNGVTQTADDRRLRQSFSATVALRNRIGS"
     gene            4141..4686
                     /locus_tag="VIW27_12220"
     CDS             4141..4686
                     /locus_tag="VIW27_12220"
                     /inference="COORDINATES: protein motif:HMM:NF025061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY5740483.1"
                     /translation="MIIHSLRPQQGVALVVSLIILVSLTLLGLSSMRRTTVDLAMAAN
                     EREIGLMFQSAEMGLITAEDYIQASTSNADFDNAPLGLYTVRAQDPTYRSPGYFNASG
                     WTAASQTAATSLEVYEQPRYKIEYLGDRSQNPLAAIGIGGYGAQQTGDVVSIYRSTSR
                     GAGLTGNSYRYLQSYYGKDKP"
     gene            4735..>7587
                     /locus_tag="VIW27_12225"
     CDS             4735..>7587
                     /locus_tag="VIW27_12225"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY5740484.1"
                     /translation="MTIIAASAMLSQATLQSAYAAPGPLATAPLFLSTIVEPNIYFTV
                     DDSGSMDWNPILGGGTDGLPTFEGLPYIDSRQRAYYSPTFSRLYNARRVLPPSNGADP
                     EWDLGWMVRNHNANLNYYNPSVKYLPWPGLKADGSPMFTDADPTNALKDPFNPAGEKI
                     DLTVTHPFSEGPKSGTLYLPTYYTWEDTDGDGIIEQTDGHTAVEIAAGTPEMQNFANW
                     FQYYRSRMNATKSIIGLTINNTSAARMGMHQFNEGQLAAVASMTDATKKRELLQSFYN
                     YDVQAQGTPMRRGLADVGDYFDNTGVDAPILQQSLGGECQRNFNILIGDGFWNGGSPT
                     VGNTDADGGSSDTIFDGNASQSNDGGNYADEVLNTLADVAMHDYERDLRSDLADRVPT
                     TPGLDLATHQHLVTFTISFGPIGSLDPAVDDPLSIGFEWPNPNSGDAEKIDDMWHAAY
                     NGRGKFLSADNPTSLARALNTAIEDIAQRTATAAAVSINSTNLTTEAVVYLAQFNSNR
                     WQGELLAFNIADTDTGELNETPRWAAAEKLNQRNIANRPRTILTHDGVKGVPFQWDSL
                     NLAQRDDLKTSPAGGVDSDDVGAARVAYLRGDRSNEGTGLLFRERASLLADLVNSGPV
                     FVGKPSLNWPDEAPFPTAVGQRYTDFKNGPAASRKGVVYAGSNGGMMHGFAESDGEEL
                     IGYISGNLYSTGSGEGLHYLTDPSYAHRYYNDLTPTVSDIYAGLGASKRWQTVLISGQ
                     RGGGRGIYALNITDPTDFSEANASKLALWELSSADDLDLGFTYSRPQIGLANNGKWVA
                     IFGNGYNDLGSGAAQLFIVDIEGGVDGSWDSGDIIKITTGSGTALDRNGLATPSLADL
                     DGNGTIDRVYAGDLRGQMWAFDLSDSLASSWGIAGDAPLFTTINNEPITSKPAIAKHP
                     TQSDDATNAPNVMVYFGSGQYLVDADKTSNDLNHFYG"
BASE COUNT         1576 a         2400 c         2140 g         1471 t
ORIGIN      
        1 ctgtcccttt cgaagtgtct gtccctttcg aagtgtctgt ccctttcgaa gtgtctgtcc
       61 ctttcgaagc gtctgtccct ttcgaagtgt ctgtcccttt cgaagcgtct gtccctttcg
      121 aagcgtctgt cccttgtccc tttcgaggtg tctgtccctt tcgatgcgct ttcgatgcgt
      181 gcaggtttta ccatgctaga caggcttaac ttagtgccat tcgtgtctgt ccctttcgaa
      241 tccccttgcg aatcctttgg ctgcacggct gcctggcgtc tgataagcgg ctgaagtcgg
      301 tgaattattt ggctggtacg gcaaaatcag gatgtttata ctgcccgcca ggtttttcga
      361 gcgcgggtaa tttctctcat gcgatctgtg ctgtttggtg aaaatcagtt ccttcgaggc
      421 cgccggtcga ttttgccggc tgcggccttg attgcctcgt ccatgctggt caccgcctgc
      481 actttcgatt ccgtggttac aacggacggc tctcagcctg tcaccgaggt aaaagtcggc
      541 gatcaatggg atacgccctt cgatcgcctg ggagtagtcc cgctgctgcc cccgagcgaa
      601 gacgtgcgtg ccggcgatat tttcgcctat cccttcaatc cggacctgca gatacgcagc
      661 agcaatcgca gccgccgcag cgaaatttcc atcagcccac gctgggattc gctcgacctt
      721 ttgcaggaac tcgaggacga atatcggctg cgcccggact ggccggcggt cgcggacagt
      781 tatccgcagt ccgcgggcgg gccgcagccg ggagaatcga gcgagcccgc cgccgcgcaa
      841 gctcgaagcc tgttctcgcc cgaccaggct ccggtgcgtt tacgcagctt cggcgtaccc
      901 gagctcgaca cttttacgct atcggacgca caggccaacg ccctggtccc gtcggcagca
      961 atcaacctgg tgttcgggtc agcatggaac gatagcaaag ccatctccat tcgcgtcaat
     1021 tcagcggaaa cctactcgct cgggctgcgc aaagtcatca gcgcggcact ggatacgtcc
     1081 ggccccgtgc aggtcctcag ggaaccgtat cggaaccagt tgtcgttgat cgccgatcca
     1141 accgccgatt cggtctggct gcggattctg agcgatgtcg tctacgttcg cagtatcgac
     1201 attattatcc agtccagatc cgcctttgac gaagacgaag tcgccatggc tagcgagttt
     1261 gtggacgaag tcgaagaaac cgttaccgtg aacgcacagg agatagctgc cgatgaggcc
     1321 agagcaaccg gggaaatgca ggcggcggca gaagacggcg aatcttccgg gaaggacccg
     1381 aaatccgaaa attcgccaat agatgcagaa atactgcgta ccgaaactat caccgaaatc
     1441 gcccccgatc atgccctgga cccggcgtac gcggcgttcg tgcgcgccga tgcgattaat
     1501 gaaaagctga tcgcggtcga tgccgacagg tcatccgcca gccagcttcg cttcgtcagc
     1561 gtcaccgacg attcggttac cgtacgcaga gtctggcagc gcggactggc tattggcgcc
     1621 cgcgggctgt ccctcgaggt agacaagctg accggcgcgg tgttgcgttc agcaaccatg
     1681 ggctcgctga tcccatagca ctcttgccac tcaccgacct tagcgtgact gacaccaatt
     1741 tccctgacga tttcctccag tctgtaatgg ccactcaaat caacttacac gggaccgaga
     1801 ttcgacagtg cgataaatcg aggcgccact gccctccata ctggccatcc tctgtcctgg
     1861 atgtgacggg tgtctcactt aaggccgctc ccgggcctgt tacctgtacc cattaccagg
     1921 tcctatgcgc acaatgccta tggtaccatc gttgcaatat gagctggtcg catgggggag
     1981 agcgaccgtt agacagcgat ctgatcgcgt tgagagagcc tgaagccgcc agtgccgttg
     2041 cgacactgca actatttaga ttttcagggg ctgggctcga ggagcaacgc ggtctgctcg
     2101 atgagaaatg atcttcataa ggttttagag tattgtgagg acgtttactg atgattgacg
     2161 cagggtgaca caatgagttc tggctacacg ctactcgaaa tactcatcgc ggtgatcctg
     2221 gtgtccctgc tgatggcatt cgccgtgcca gcgatgcggg catttaccca aaacgaccgc
     2281 atgatcacca atgtcaacac cctgatcggt cacttgaact atgcgcgcag cgaagcagtc
     2341 aagcgcagcc agcaggttac cgtctgcgcc agcaacgacg ccaccacctg cagcggcacc
     2401 tggtccgacg gctggatagt ctatgtcgat gccgacgcgg acaacagttt taccgccggc
     2461 gaggaaatca ttcgggtcaa gcagtcgctg gaaggcggta actcgctgac cgcgaccgac
     2521 atcggcggcc agatcaccta taacaatcgg ggcttcgtca acgacgcctc ggtcggcagc
     2581 ctgcaactgt gcgacgatcg cagcggtgcc tatggcaaga ccgtgcgcat caccagcctc
     2641 ggacgcgtca ggctggaggt agatacgtca tgttgacccg gtcccggcag cagcggggta
     2701 tgaccctgat cgaggcgatg attgcgctgc tggtgatttc cgtcggcctg ctgggtatcg
     2761 cgtcactgca attgacctcg atgaaacaga atgtcagtgc gctcaatcac agccgcgcgg
     2821 tatggtacac ctacagtatg tccgaccgga ttcgcgccaa catcgacgag ttcgccaact
     2881 acgacggtat cgataccagt ggagaatatg cacaggattg catgtcgcag ccctgcagca
     2941 ctgcacagat gcgtattgcc gatgccgccg actggtcggc catggtctcc aacctgcccg
     3001 ccggccgtgg catcatcgcc agcaatgccg acggcctgat ggtctcggtc atgtgggatg
     3061 acgaaggcac cggggccacc ggtaccggct gcgggccgga tgcgaacgtc gacctgactt
     3121 gctacagcct cgaggtgggg caatgaacgg cgtcaatcgt caacgcggca tttcgctggt
     3181 cgagctgctg atcgcgatgg tgatcggcct gttcctgctg gccggtatct accaggtgta
     3241 tatcagcaac aaagcgacct ttgcatttac caatgccatt gccgaagtgc aggagaatgg
     3301 ccgcttcgcg ctcgacctca tgtcgcagga cctgcgcatg gcctcggaat ggggctgcat
     3361 ccagctggat ccgaaggata ccgctaatat caatgacacg ctcagtgcca cgaccctgcc
     3421 cggctacgac accgagtttc acgacttcat cggaaaatcg ggcatcgagg gtaacaacaa
     3481 tactggtttg aacaactccg acacgctgac cattcgtggc ggcaggccca gccaggccaa
     3541 cgtcgagggc cccttctttc cgccgacctc gaaacagctc gtcaccgatg ccgtcaatag
     3601 catcggtatc ggcgatatcg tgctggtggc gcgctgcggc gccaacgatc tgctgatcga
     3661 gacggaggcc gatatcttca gggtgaacga gacgtttccg ttcaacaaca acacgcagcg
     3721 cgtgctcacg ttctcggcga acaagagcca gcaattcgaa aacgatgcga tggtcatcgt
     3781 actgcagacc gtcaactact tcatcgccgc cggcgagagc ggcgagcccg ccctgtttcg
     3841 ccgggagttc ggtaatgcgc cgcgggaatt gatcgagggt accgaagacc tgcaaatcct
     3901 ctacggcgtc gataccgaca acgacgattt tccgaatcag tatctgaccg ccgacaaggt
     3961 gctcaacttc aacgacgtgg tcgcggtgcg cgtgatgctg ctggtccgct cggtcgacga
     4021 taacgtcacc gacactccgc agacttacac ctacaacggc gtaacgcaga ccgccgacga
     4081 ccggcggctt cgccagtcgt tttcggcgac cgttgccctc agaaaccgca taggatcctg
     4141 atgatcattc actcgttacg accgcagcaa ggcgtcgcgc tggtagtcag cctgatcatc
     4201 ctggtcagcc tgaccctgct cggcctgtca tcgatgcgcc gcaccacggt cgacctggcg
     4261 atggccgcca acgagcgcga aatcgggctg atgttccagt ccgccgagat gggcctgata
     4321 acggcggaag actatatcca ggcgtcgacc tccaatgccg atttcgacaa tgctccgctc
     4381 ggcttgtata ccgtgcgggc ccaggacccg acctacagga gtcccggtta tttcaacgcc
     4441 agcggctgga cagctgcgtc gcaaaccgcg gcaacttcgc tcgaggtata cgaacagccg
     4501 cgctacaaga tcgagtacct gggggaccgt tcccagaacc cgctcgccgc tatcggcatc
     4561 ggcggctacg gtgcgcaaca aaccggcgac gtggtttcga tttaccgttc gacctcgcgc
     4621 ggcgccggcc tgaccggtaa ctcttaccgt taccttcagt cttactatgg caaggacaag
     4681 ccctaggctg ccttcaggat cgatatcgtg attagcagaa aaatttccag aacaatgacg
     4741 ataatcgcag ccagcgcgat gctgagccag gcgaccctgc aatcggctta cgccgccccg
     4801 gggccgctgg caactgcccc gctgttcctt tcgaccatcg tcgaacccaa catctacttc
     4861 acggtggacg actccggcag catggactgg aacccgattc tcggcggcgg taccgacggc
     4921 ctgccgacct tcgaaggcct gccgtatatc gacagccgcc agcgcgccta ctattcgccg
     4981 accttctcgc gcctgtacaa cgcgcgccgg gtactcccac cctccaacgg cgccgatccc
     5041 gaatgggatc tgggctggat ggtgcgcaac cataatgcca atctgaacta ctacaacccg
     5101 tcggtgaagt acctgccgtg gcccggcctc aaagccgacg gctcgccgat gtttaccgac
     5161 gccgacccga ccaacgcgct caaggatccg ttcaaccctg ccggtgaaaa aatcgacctg
     5221 accgtgacgc accctttcag cgagggcccg aaatccggca ccctgtacct gccgacctac
     5281 tacacctggg aggataccga cggtgatggc atcatcgagc agaccgatgg ccataccgcg
     5341 gtagagatag cggccggcac ccccgagatg cagaacttcg ccaactggtt ccagtattac
     5401 cggtcgcgca tgaacgccac caagtcgatc atcggcctga cgatcaacaa tacgagcgcg
     5461 gcgcgtatgg gcatgcacca gttcaatgaa ggtcagctgg cggcggtggc atcgatgacc
     5521 gacgcgacca agaaacgcga gctgctgcag agtttctata actacgacgt ccaggcgcag
     5581 ggcaccccga tgcgccgcgg cctcgccgac gtcggcgact acttcgacaa tacaggcgtc
     5641 gatgccccga tcctgcagca atcgctgggc ggcgaatgcc agcgaaactt caatatcctg
     5701 atcggcgacg gcttctggaa cggcggctcg cccacggtcg gcaatacgga tgccgacggc
     5761 ggcagcagcg atacgatttt cgatggcaat gcatcgcagt ccaacgacgg cggcaattat
     5821 gccgatgaag tgctgaatac gcttgccgac gtcgccatgc acgattacga gcgggacctg
     5881 cgcagcgatc tcgctgaccg tgtgccgacc acgcccggcc tcgacctggc cacccatcag
     5941 cacctggtga cctttaccat ttccttcggt cccatcggca gcctcgatcc ggcagtcgac
     6001 gacccgctgt cgatcggttt cgagtggccc aacccgaaca gcggcgatgc cgaaaaaatc
     6061 gatgatatgt ggcacgctgc ctataacggc cgcggcaaat tcctcagcgc cgacaacccc
     6121 acctcgctgg ccagggcact gaataccgcc atcgaggata tcgcgcaacg caccgcgacc
     6181 gcggccgcgg tatcgatcaa ctcgaccaac ctgacgaccg aggcggtcgt ctacctggcg
     6241 caattcaatt ccaaccgctg gcagggcgag ctgctggcgt tcaatatcgc cgataccgat
     6301 accggcgagc tgaatgaaac accgcgatgg gctgccgcgg aaaagctcaa ccagcgcaat
     6361 atcgccaaca ggccgcgcac catcctgacc catgatggtg tcaagggggt acccttccaa
     6421 tgggacagcc tgaacctggc acagagggac gacctgaaaa ccagccccgc tggcggcgtc
     6481 gacagcgatg acgtcggcgc ggcgagggtc gcctacctgc gcggcgatcg cagcaacgag
     6541 ggaaccggcc tgttgtttcg cgaacgggca tcactgctcg cggacctggt caactcgggt
     6601 ccggtcttcg tcggcaagcc gtcgctcaac tggcccgacg aggccccctt cccgactgcc
     6661 gtcggccaac gctacacgga cttcaagaac ggtcccgcgg cttcgcgcaa gggtgtcgtc
     6721 tacgccggtt ccaacggcgg catgatgcac ggttttgccg aatccgacgg cgaagaactg
     6781 atcggctata tttccggcaa cctgtattcc accggcagcg gcgagggcct gcattacctg
     6841 accgatccga gctacgcgca ccgctactat aacgacctga cgccgaccgt ttcagacatc
     6901 tatgccggtc tcggcgccag caagcgctgg caaaccgtat taatcagcgg ccaacgcggc
     6961 ggcggtcgcg gcatctatgc gctcaacatc accgatccga cggatttttc cgaggccaac
     7021 gccagcaaac tcgctctctg ggaattatcc agcgccgacg atctcgatct cggctttacc
     7081 tacagccgcc cgcagatcgg gcttgccaat aacggcaagt gggtagcgat tttcggcaac
     7141 ggctacaatg atctcggcag tggcgcagcg cagctgttca tcgtcgatat cgagggtggc
     7201 gtcgacggta gctgggacag cggcgatatt atcaagatca ccaccggttc cggtaccgca
     7261 ctcgaccgca acggactcgc cacgccgtca ctggccgatc tcgacggcaa tggcaccatc
     7321 gatcgcgtct acgccggcga tctgcgtggc cagatgtggg ctttcgacct ctccgattcg
     7381 ctcgccagca gctggggaat tgccggcgac gcaccgctgt tcacgaccat caacaatgag
     7441 ccgattacca gcaaacccgc gattgccaag cacccgacgc agtccgacga tgccaccaat
     7501 gcgcccaatg tcatggtgta cttcggttcc gggcaatacc tggtcgacgc cgacaagacg
     7561 agcaacgacc tgaatcattt ctacggc
//
LOCUS       DATCZV010000542         2885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_626560, whole genome shotgun sequence.
ACCESSION   DATCZV010000542 DATCZV010000000
VERSION     DATCZV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626560"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIW27_12230"
     CDS             <1..284
                     /locus_tag="VIW27_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740485.1"
                     /translation="PIQSMLTIALVFHWQYGGDSPALWPSLVTWLKIVAYMLLAWLIT
                     REAGIRLSRYIDRWLTVSGSIRLVTDTLYMLLQIPVMLIYGEYLSAQLA"
     gene            complement(291..974)
                     /gene="lolD"
                     /locus_tag="VIW27_12235"
     CDS             complement(291..974)
                     /gene="lolD"
                     /locus_tag="VIW27_12235"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEY5740486.1"
                     /translation="MEPAAIEARAVSKRFRQGKLDVEVLKGVDFRVVRGESHAILGAS
                     GAGKSTLMHLLGGLDHVSDGAIRVAGKDLGKLSEAELGRLRNRHLGFIYQFHHLLPEF
                     SALDNIAMPLLIRRMPYRDARATAAEWLQKIGLEQRSTHKPGELSGGERQRVALARAL
                     VSRPDCLLADEPTGNLDRQNAARMLDLMLELNRELQTALVVVTHDLEIAARMQYRWRL
                     RDGILDAED"
     gene            complement(987..2234)
                     /locus_tag="VIW27_12240"
     CDS             complement(987..2234)
                     /locus_tag="VIW27_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY5740487.1"
                     /translation="MNLPLEIFVGLRYTRAKRRNHFISFISLISMLGIALGVMALIVV
                     LSVMNGFEKELRGRILGMVSHVTISSFRGPLDDWQSLRARALEQADVIGAAPYIEAEA
                     MISNLSSVSGALIRGIDPELEVEVSEIHRHMQFGELADLVAGGYGIVLGSGLANTLDV
                     VPGDRVTMVTPQVTASPLGFLPRLRRFEVVGIFEIGVYEYDRSSALIHHQDASRLFRL
                     DGGVNGLRLKLDDLDLAPAVREELKQQIGLQYWVSDWTLRHSNYFKAVRTEKTVMFII
                     LSLIVAVAAFNIVSTLVMVVTDKQADIAILRTLGMSPTSIMWVFMVQGTLIGLIGTLA
                     GLASGVAVASHIDVIIPALERFFQTQFLPRGVYPITDLPSEMKQSDIVRITLLSFGIS
                     VVATLYPALRASKTRPAEALSYE"
     gene            2403..2699
                     /locus_tag="VIW27_12245"
     CDS             2403..2699
                     /locus_tag="VIW27_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740488.1"
                     /translation="MRTGIFMILLGSSLLLQSASAGDQLTIPGHVPASQSQPMPQRGI
                     SMDDVLSQFGEPDERFGPVGEPPITEWVYGSFRVYFEHQIVLHSIDLNTLIMPK"
BASE COUNT          577 a          893 c          877 g          538 t
ORIGIN      
        1 tgccgattca aagcatgcta accatcgcgc tggtatttca ctggcagtac ggcggcgatt
       61 caccggctct gtggccgtca ttggtcacct ggctgaagat cgtcgcctac atgctgctcg
      121 cctggctgat cacccgcgaa gccggtattc ggctgtcgcg gtacatcgat cgctggctga
      181 cggtcagcgg cagcatacgg ctggtcaccg acacgctgta catgctgctg caaatcccgg
      241 tgatgctgat atacggcgaa tatctcagcg cacaactggc ctgaacggct tcagtcctcg
      301 gcgtcgagaa tgccgtcacg caggcgccag cgatactgca tgcgcgcggc aatttcgaga
      361 tcgtgggtga ccaccaccag cgcggtctgc aactcgcgat tcagctccag catcaggtcg
      421 agcatgcgcg ccgcgttctg ccggtcgagg ttgccggtcg gttcgtccgc cagcaggcag
      481 tcgggccgcg acaccagcgc cctcgccagt gccacgcgct ggcgttcgcc gccggataat
      541 tctccgggtt tgtgagtgct gcgctgctcg agtccgattt tttgcagcca ttctgccgcg
      601 gtggcgcggg cgtcgcggta tggcatgcgc ctgatcagca gcggcatggc gatattgtcg
      661 agcgcactga attcgggcag caggtgatgg aactggtaaa tgaacccgag gtgacggttg
      721 cgcaagcgcc cgagttcggc ttcggacagc ttgccgagat ccttgccggc gacgcggatc
      781 gcgccgtcgc tgacgtgatc gagcccgccg agcaggtgca tcaaggtcga tttcccggct
      841 ccggacgcgc cgagaatggc gtggctttca ccgcgcacga cgcgaaagtc gacgcccttg
      901 agcacctcga cgtcgagctt tccctggcga aatctcttgc tgaccgcgcg ggcctcgatg
      961 gcggctggct ccactagctc ggtcgcctat tcataagaca gagcctcggc cgggcgcgtc
     1021 ttcgatgccc tgagcgccgg atacagtgtc gcgaccaccg atatgccgaa cgacaacagc
     1081 gtaatacgca cgatatccga ctgcttcatt tccgagggca ggtcggtgat cgggtaaacg
     1141 ccgcgtggca gaaactgggt ctgaaagaat cgctccagcg ccggaataat gacatcgata
     1201 tgcgaggcga cggcgacccc gctcgccagc cccgccagcg taccgatcag cccgatcagg
     1261 gtgccctgga ccatgaacac ccacatgatc gatgtcggcg acatgccgag agtacgcagg
     1321 atggcgatgt ccgcctgctt gtcggtaacc accatcacca gcgtggagac gatattgaac
     1381 gcggccaccg cgacgatcag cgacaggatg atgaacatca cggttttctc ggtgcgcacc
     1441 gccttgaaat aattgctgtg tctgagggtc cagtcggaga cccagtattg caggccgatt
     1501 tgctgtttca gctcttcgcg gaccgccggc gccaggtcga gatcgtcgag cttgaggcgc
     1561 aggccgttga cgccgccgtc gagccgaaac aggcggctgg catcctggtg atggatcaac
     1621 gcgctgctgc ggtcgtactc gtagacaccg atttcgaaaa tgcccaccac ctcgaagcgc
     1681 cgcaggcgcg gcaaaaagcc caacggtgac gcggtgacct gcggcgtgac catggttacg
     1741 cggtcgccgg ggaccacgtc gagggtattg gccagtccgc tgccgagcac gatgccgtag
     1801 ccgccggcga ccaggtcggc gagttcaccg aactgcatgt gccggtggat ttccgagacc
     1861 tcgacctcga gttcgggatc gatgccgcgg atcagcgcgc cgctgaccga cgacaggttg
     1921 gagatcattg cctcggcctc gatgtagggc gcagcgccga tcacgtcggc ctgctcgagg
     1981 gccctggcgc gcaggctttg ccagtcgtcg agcggcccgc gaaaactcga tatggtgacg
     2041 tgggatacca tgcccagaat gcgcccgcga agttccttct cgaagccgtt catcaccgac
     2101 agcacgacga tcagcgccat cacgccgagc gcgataccga gcatcgagat cagcgatatg
     2161 aaagagatga aatgattgcg tcgcttggcc cgcgtgtaac gcaggccgac gaagatttcc
     2221 agcggtaaat tcatgcggcg gattaaacca cgaatgaccg acgcccacca ccgtaaccgc
     2281 aagcgtctcg cgcatcgcga agatcaacgt cagagcgggg tttcgccgac tcgccgcggc
     2341 gctcttgcct gcactccggg gcgtgctata gttaatctca atgcattagg agacccgata
     2401 acatgcgcac gggaatattt atgatcctgc tcggctcgag cctgttgctg caatcggcga
     2461 gcgctggcga ccagttgacg atccccggcc acgtgccggc ctcgcaatct cagccaatgc
     2521 cgcagcgagg catcagtatg gacgacgtac tgagccagtt cggcgaaccc gacgagcgtt
     2581 tcggaccggt cggcgaaccg cccatcaccg aatgggtcta cggctctttc agggtatatt
     2641 tcgagcatca gatcgtgctg cacagcatcg acttgaatac cctgataatg ccaaaataga
     2701 atacccaaaa ataatcgcgg ccctaaccag gccgcaatgc cgataatgtc ggcaattata
     2761 accggagcca cgttcatgaa cccgaaaacg attctgtgcc tcgccgcgct cataccggtc
     2821 ctcgtggtac cgggtgtgta tgcggccgag gtcaatatca gccccgagat cgaaagcgtt
     2881 accgt
//
LOCUS       DATCZV010000543         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_256096, whole genome shotgun sequence.
ACCESSION   DATCZV010000543 DATCZV010000000
VERSION     DATCZV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256096"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VIW27_12250"
     CDS             <1..688
                     /locus_tag="VIW27_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEY5740489.1"
                     /translation="STRFATVLLISSLLAAWPAAYARAADEVMYVTDTFEVTMRSGTS
                     TANSIIRILKSGEPLTVVEHDLASQYSLVRSADGKQGYVLSRYLATEPSAREQLADMR
                     QSLQERDERIAALQGEIAQLQQTLEREQADNQTLGTSLRTTEEQLADVRDATRDSLEI
                     IEQNQRLQTVVDELSAEKAALSAANAELGDTTRLDWFMRGGAVSLIAFLIGILVTRIR
                     WRKQDSWGSY"
     gene            complement(713..1777)
                     /locus_tag="VIW27_12255"
     CDS             complement(713..1777)
                     /locus_tag="VIW27_12255"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEY5740490.1"
                     /translation="MPLVAHNDLPSFERLRREGVRILDQGLATHQDIRELHIGLLNMM
                     PDAALGATENQFFRLIGESNPIAQFYVHPFTLDALPRAAAAREHIDRYYESFEQIREQ
                     GLDALIITGANVIGADLSEEVFWKPLIEVADWAHENVTSTLCSCLATHAVMDFRYGQK
                     RSKQKRKTWGVYRHRVVETGHPLITDINTEFDVPHSRWNDISRAQFEAAGLRVLVTGE
                     GDCVHLATSPDGFRSVFFQGHPEYDTISLLKEYKRDVNLYIDGEIREYSPMPDNYFSS
                     FAAAVMREYRMRVDSCLAAGRPVPEFPEHLVVDTLKNTWRDTASAVVGRWIGLGYQYT
                     GIDRLQPFMEGVDSDNPLGL"
     gene            complement(1777..2634)
                     /locus_tag="VIW27_12260"
     CDS             complement(1777..2634)
                     /locus_tag="VIW27_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY5740491.1"
                     /translation="MKFSVDEFRAWENKCVTLVGMSGVGKSYLSAKLRGSNWFHYSGD
                     YRIGTRYLDEHIIDMLKSQAIRIPFLMELLRNDWIYIENNIRVNDLGPVLSFVGKLGN
                     PELGGVELGEFIERQEIYRQAEVDAMYDIPRFIDKARSIYGYPHFVNDVGGSLCELDE
                     PGVMDILVDNTLIVYIKVTDDAQEQVLIERAVSDPKPLYYRPDFLQQHLEMYFAETGL
                     EYAAQIDPDEFARWVFPHLFHSRLPRYDEIANLGYTVTSGEVDQVQNDEDFLQLLELA
                     IDRESGEGA"
     gene            2758..>3264
                     /locus_tag="VIW27_12265"
     CDS             2758..>3264
                     /locus_tag="VIW27_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5740492.1"
                     /translation="MELEFDYVIVGAGSAGCVLANRLSDGGRFRVLVLEAGGSDRRFW
                     IQTPIGYGKTFYDPAVNWKYMTESEPGINGRRLYWPRGKVVGGSSSINAMVYIRGQQQ
                     DYEDWRELGNPGWGWRDVLPYFRKSETCATGGNDYRGDGGPLYVSDPHAEYHPLCKHF
                     FTAAGQLGI"
BASE COUNT          653 a          993 c          984 g          634 t
ORIGIN      
        1 ctcgacacgt tttgcgacag tcttgttgat ttcttcgctg ctggccgcct ggcctgccgc
       61 gtacgcgcgc gcggccgacg aagtcatgta tgtcaccgat accttcgagg ttacgatgcg
      121 cagcggcacc agcaccgcca acagcatcat tcgcatactc aagagcggcg aaccgttgac
      181 tgtcgtcgag cacgacctgg cctcgcaata ctcgctggtg cgctcggccg acggtaaaca
      241 gggctacgtg ctgagccgat acctggccac cgagccgtcg gctcgagaac agctggccga
      301 tatgcggcaa agcctgcagg agcgcgacga acgcatcgcc gcgttgcagg gcgaaatcgc
      361 gcagctgcag caaacgctgg agcgcgaaca ggccgacaac cagaccctgg ggacgagcct
      421 aaggactacc gaggaacaac tcgccgacgt acgtgatgcg acgcgcgaca gcctcgagat
      481 catcgagcag aaccagcgcc tgcaaactgt ggtcgatgaa ttgagtgcgg aaaaggcggc
      541 gctgtcggcg gccaacgccg agctcggcga caccacccgc ctggactggt tcatgcgcgg
      601 cggtgcggta tcactgatcg cgttcctgat cggcatcctg gtcacacgta ttcgctggcg
      661 caaacaggac tcgtggggat cctactgagt cttgcgtcgt ctcggggcgc ggctaaagac
      721 cgagcggatt gtcgctgtcg acaccttcca taaacggctg cagacggtcg atcccggtat
      781 actggtaacc cagcccgatc cacctgccga caacggcgct ggcggtatcg cgccaggtat
      841 ttttcagcgt atcgaccacc aggtgctccg gaaactcggg caccggccgg cccgcggcca
      901 ggcaggaatc gacgcgcatt cgatactcgc gcatcaccgc ggcggcgaag ctgctgaagt
      961 aattgtccgg catcggcgag tactcccgta tctcgccgtc gatgtacagg ttgacgtcac
     1021 gtttgtattc cttcaacagc gagatggtgt cgtactccgg atggccctgg aaaaacacgc
     1081 tgcgaaaacc gtcaggactg gtggccaggt gcacgcagtc gccttctccg gtcaccagca
     1141 cccgcaaccc ggctgcctcg aattgcgcgc ggctgatgtc gttccagcgc gaatgcggca
     1201 cgtcgaactc ggtgttgata tcggtaatca gcgggtgtcc ggtctcgact acgcgatgcc
     1261 ggtagacgcc ccacgtcttg cgtttctgct tgctgcgctt ctggccgtaa cgaaaatcca
     1321 tcaccgcatg cgtcgccagg cacgagcaca acgtcgaagt cacgttttcg tgcgcccagt
     1381 cggcgacctc gatgagcggc ttccagaaca cttcctcgga gaggtcggcg ccgatgacgt
     1441 tggcgccggt gatgatcaac gcatcgagcc cctgctcgcg tatctgttcg aaactctcgt
     1501 agtaacgatc gatgtgttcg cgcgccgcgg cggcgcgcgg cagcgcgtcg agcgtaaacg
     1561 gatgaacgta aaactgcgcg atcgggttgc tttcgccaat cagcctgaaa aactggttct
     1621 cggtcgcacc gagcgcggca tcgggcatca tgttgagcag tccgatatgc agctcgcgaa
     1681 tatcctgatg agtggctagc ccctgatcga gtatgcgcac gccctcgcgg cgcagacgct
     1741 cgaaactcgg caaatcgttg tgtgcgacca gtggcatcag gcgccctccc ccgattccct
     1801 gtcgattgcg agctcgagca attgcaggaa atcctcgtcg ttttgtacct ggtcgacctc
     1861 gccggaggtg accgtgtaac cgagattggc gatctcgtcg tagcgcggca gccgcgaatg
     1921 aaacaggtgc ggaaaaaccc agcgcgcaaa ctcatccgga tcgatctgcg cggcatactc
     1981 gagaccggtc tcggcaaaat acatttcgag gtgctgctgc agaaaatcgg gacggtaata
     2041 cagcggtttg ggatcggaaa ctgcgcgttc gatcaacacc tgctcctggg catcgtcggt
     2101 gaccttgatg tagacgatca gcgtattgtc gaccaggata tccatgaccc ccggttcgtc
     2161 gagttcgcac aggctgccac cgacgtcgtt gacgaagtgc gggtagccgt aaatgctgcg
     2221 cgccttgtcg atgaagcgcg gaatatcgta catcgcgtcg acctcggcct ggcgatagat
     2281 ctcctggcgt tcgatgaatt cgccgagttc gacaccgccg agttccgggt tgccgagctt
     2341 gccgacaaag ctcagcaccg ggcccaggtc gttgacgcgt atgttgttct cgatgtagat
     2401 ccagtcgttg cgcagcaatt ccatcagaaa cgggatgcga atcgcctgcg acttgagcat
     2461 atcgatgatg tgctcgtcga ggtaacgcgt gccgatgcgg taatcgcccg agtagtggaa
     2521 ccagttgctg ccgcgcagct tggccgacag gtaggacttg ccaacgcccg acatgccgac
     2581 cagcgtgacg cacttgtttt cccaggcacg gaattcatcg acgctgaatt tcaaatctgc
     2641 ctccatcgaa tcgagccgca gattatgcat caagcggcgg gccggcgaaa caccagaata
     2701 tgctttaccc gccgagtgca aggtctataa tccgatttca ttttctcggg cggcagcgtg
     2761 gaactcgagt tcgactatgt gatcgtgggc gcgggttcgg ccggatgcgt gcttgccaac
     2821 cgcctcagcg atggcggcag gttccgcgtg ctggtacttg aggccggcgg tagcgaccgg
     2881 cgtttctgga tccagacacc gatcggttac ggcaagacct tttacgatcc cgcggtcaac
     2941 tggaaataca tgaccgagtc cgaacccggc atcaacggac gacgcctcta ctggccccgc
     3001 ggcaaggtcg tcggcggttc cagctcgatc aacgcgatgg tctacattcg cggccagcaa
     3061 caggattacg aggactggcg cgaactcggc aaccccggct ggggatggcg cgacgtgctg
     3121 ccctactttc gaaagtcgga aacctgcgcc actggcggca acgactaccg cggcgatggc
     3181 ggcccgttgt acgtcagcga cccgcatgcc gagtatcatc cgctgtgcaa acattttttt
     3241 accgccgccg gccagttggg catt
//
LOCUS       DATCZV010000544         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_203097, whole genome shotgun sequence.
ACCESSION   DATCZV010000544 DATCZV010000000
VERSION     DATCZV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203097"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VIW27_12270"
     CDS             complement(<1..1277)
                     /locus_tag="VIW27_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY5740493.1"
                     /translation="MPRHAVAILAGALLAVYGNQLPDRAWSSYLPLLLLLWRFCPHWR
                     WLLLAAAAHLWSLALLHIHIDHRLQDAYDDRVVLLRGSVDGIPQQRQGRVSFYLDEVA
                     IEGYPGRLPRRVRLDWYHNELLPLPGERWQLVVKLRQPRGALNPAGFDFTAWQFANGI
                     DAGGHVRDSPANRRLAPAGWFDTHRWRLRLAQAIDESCGDCPRAGLLKALAIGYRGAI
                     ESRHRRLLERSSTAHLLAISGLHIGMVALFAYALGRGAWRLGLYRVIISRPYFAALLA
                     VICACGYAAMAGFSLPTVRALVMLLVLSIALLLRNRVNLLQSLAIAVATITLADPRSL
                     ISSSFWLSCGAMLVIAFAQFRVPQAMPGWRRLLVVQIFFTLLFAPVGVMIFGQWAPAS
                     LPANLLAIPVVSFLLLPAILLGSLLAAGGLPGGG"
     gene            1358..1903
                     /locus_tag="VIW27_12275"
     CDS             1358..1903
                     /locus_tag="VIW27_12275"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY5740494.1"
                     /translation="MAKKFLRKFLPHPDVITQNRGIKLLGPRLQDPSLWHINRRSFSL
                     AVALGMFCAFIPVPFQMVIAAAAAIWARVNLLVAVPMVWISNPFTMGPLYYFCYRVGA
                     RMLGVEPQKFHFELSFEWLMTGLARVWEPFLLGCFAVGAISALVAFVAVRILWHLHIL
                     AHIKERTRRLHNRRHPHEDGS"
     gene            1900..>2180
                     /locus_tag="VIW27_12280"
     CDS             1900..>2180
                     /locus_tag="VIW27_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740495.1"
                     /translation="MNLLLPAIIVAGSVLGGVSLSFYLLHKAHQTDTLVWYLEHVLCP
                     LLRVIVLLIVVSQVYPAIDSQSTSLDFWRHLRERQQLQDIVNILFFGGLL"
BASE COUNT          443 a          696 c          674 g          367 t
ORIGIN      
        1 ccgcctccgg gcaggccgcc ggcggccagc aagctaccga gcaatatcgc cggcaacagc
       61 aggaaactga ccaccggtat cgccagtaga ttggccggca ggctcgccgg cgcccactgg
      121 ccgaaaatca tcacgccaac gggtgcgaac agcaaggtaa agaaaatctg cacgaccagc
      181 agccggcgcc agcccggcat ggcctgcggt acgcgaaact gcgcgaaggc gatgaccagc
      241 atcgccccgc agctcaacca gaaggaactc gaaatcagcg aacgcgggtc ggccagcgtg
      301 atcgtcgcaa ccgcgattgc cagcgattgc agcaggttga cgcgatttct cagcaacagc
      361 gcgatcgaca gcaccagcag catgaccagc gcgcgcaccg tcggcaggct gaagccggcc
      421 attgccgcat agccgcaggc acagatcact gccagcagcg ccgcgaaata cggccggctg
      481 atgatcacac ggtaaagacc cagccgccat gcaccccgac ccagcgcata ggcgaataac
      541 gccaccatgc cgatatgcag accggaaatc gccagcaggt gcgcggtcga actgcgctcg
      601 agaaggcgcc gatgccgact ttcgatagca ccgcggtagc cgatcgccaa cgccttgagc
      661 agaccggctc gcgggcaatc gccacagctt tcgtcgatag cctgcgccag gcgcaggcgc
      721 cagcgatgcg tatcgaacca gccggccggc gcgagccgcc ggttggcggg cgaatcgcgc
      781 acgtggccgc cagcatcgat accgttagcg aattgccagg cagtgaaatc gaaaccggcc
      841 gggttcagcg cgccgcgcgg ctggcgcagc ttgaccacca gctgccagcg ttcacccggc
      901 agtggcagca gctcgttgtg gtaccagtcg agacgcacgc ggcgcggcaa gcggccgggg
      961 tagccctcga tagcaacctc gtcgaggtag aagctgacgc gaccctggcg ttgctgcgga
     1021 ataccatcga cgctgccccg cagcaacacc acgcgatcgt cgtaggcgtc ctgcaggcga
     1081 tggtcgatgt ggatatgcag cagggccagg ctccacaggt gcgcggccgc ggccagcaac
     1141 agccagcgcc agtggggaca aaaacgccac aggagcaata gcaaaggcag gtaggacgac
     1201 caggcgcgat cgggcagctg gttaccgtag acggccagca aagcacccgc gagaatggcg
     1261 actgcgtgtc ggggcatgcg atccttgcgc tgttttccct gtcaaatcga tccagcgctt
     1321 tacagcatat tcagttactg ccgataatac aaacagcatg gcaaagaagt tcctcagaaa
     1381 gtttttgccg catccggacg tcattaccca gaaccgtggc atcaagcttc tcgggccgcg
     1441 gttgcaggat ccgtcattgt ggcacatcaa ccgacgctcg ttttcgctgg cggtcgcgct
     1501 tggcatgttc tgcgccttca taccggtccc gttccagatg gtcatcgccg cggcggccgc
     1561 catctgggcg cgcgtcaacc tgctggtggc ggtgccgatg gtctggatat ccaacccgtt
     1621 cacgatgggg ccgctatatt atttctgtta ccgggtcggt gccagaatgc tcggagtcga
     1681 accgcaaaaa tttcatttcg agctgagttt cgagtggctg atgaccggcc tggcgcgggt
     1741 atgggaaccg tttctgctgg gttgcttcgc ggtcggggcg atcagcgcgc tggtggcttt
     1801 cgtggcggtg cgcattctat ggcacctgca catcctggcc cacatcaagg agcgcacccg
     1861 gcgcctgcac aatcggcgcc acccgcacga ggacggctcg tgaatctgct gttgcccgcc
     1921 atcatagtcg cgggcagtgt gctgggcggg gtcagcctga gtttttacct gctgcataaa
     1981 gcccaccaga cggataccct ggtctggtat ctggagcacg tcttatgccc gctgctgcgc
     2041 gtgatcgtgc tgctgatcgt cgtcagccag gtttacccgg caatcgacag ccagtcgacc
     2101 agcctcgatt tctggcgcca tctgcgtgaa cggcagcaat tacaggacat cgtcaacatc
     2161 ctgttcttcg gcggcctgct
//
LOCUS       DATCZV010000545         6449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_96644, whole genome shotgun sequence.
ACCESSION   DATCZV010000545 DATCZV010000000
VERSION     DATCZV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96644"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VIW27_12285"
     CDS             complement(<1..1040)
                     /locus_tag="VIW27_12285"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013960550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5740496.1"
                     /translation="MAKRVAVIGAGPSGLAQLRAFQSANEKGADIPEIVCFEKQADWG
                     GLWRYTWRTGLDEHGEPVHGSMYRYLWSNGPKEGLEFADYFFEDHFGKPIASYPPRAV
                     LFDYIEGRVKKAGVRQWCRFNTPVRHVSYDDANGNFRVTAHDLDKDIEYTEEFDHVIV
                     ASGHFSTPNVPYFEGFETFNGRVLHAHDFRDAMEFRDKDILIIGTSYSAEDIGSQCWK
                     YGCKSVTVSHRTAPMGYKWPDNWKEVPLLQKVEKNTATFKDGSTAEVDAIILCTGYLH
                     HFPFMAEGLKLRTANRLATADLYKGVAWCDNPKLFYLGMQDQWYTFNMFDAQAWWVRD
                     VILGRIELPDNK"
     gene            1259..1891
                     /locus_tag="VIW27_12290"
     CDS             1259..1891
                     /locus_tag="VIW27_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5740497.1"
                     /translation="MADKEAEIADIETAIEQHSVEALEIAIGKQVRASRKRLHLTVAA
                     LAKQAQLSTGMLSKIENGHTSPSLATLTSLARALQVPVTSFFRGYEEQRDVTFIKAGD
                     GLPIERRGSGAGHQYQLLGHTIGKPYNIEPYLITIDDQSEVFPVFQHSGMEFIYMLEG
                     RVIYRHANQTYTLEAGDSLFFDAEASHGPDEIQKLPCRYLSIIVSESTTS"
     gene            1992..2900
                     /locus_tag="VIW27_12295"
     CDS             1992..2900
                     /locus_tag="VIW27_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY5740498.1"
                     /translation="MCGIAGVLFKHQGAHEIGKTLIEMLDGCQHRGPDSTGFALYGDN
                     KELLQLRFIVPTEDAARHEAIDNIATLLERHGASIDSEQSVGCSYQIAVRFDGDLLAF
                     TKDIEEHAKLVSVGHTLDIIKDDGTAHELDELYHVGEFRGTHGIGHVRLATESAVRPE
                     AAHPFWATGFEDIAIVHNGQITNYWKMRRRLERRGYSFHTDNDSELVAVYLADKLSQG
                     ETLDHVLETAIADLDGTFSFLVSTRDEIGYAKDNLAAKPMVLYETDDMIAIASEEVSL
                     NRLFPGQSINSTEPAPGTHQTWSRSI"
     gene            2882..4864
                     /locus_tag="VIW27_12300"
     CDS             2882..4864
                     /locus_tag="VIW27_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY5740499.1"
                     /translation="MVQIDLSRVAITEANDKIRAFAADRQEVEIINPDARHNIGVGLV
                     VDDPITVRVRGSAGYFCGGLTHGVRFEIEHNAGWAVGDNIYQGTVVVGGNAGAIPGVA
                     IRGAEIVVRGNMGSRAGQVMKAGTLACGGNAAFMAGYMMYGGRIIILGDAAAKVGQDM
                     SAGEIFVGGKIESLGNDTMIVDMEDRERDEIMEFLDRFELKHHGAFTKIVNAGKKLRY
                     AKAEPRHRPQPFFVSSKSSNYWNAKVQEDIWIKGEVGRYRIRGYGASKPVPHLNDIAF
                     VKDVSSVAPDPGELKDINLKTTVGGRFGAKPISLSMPVMIAPMSFGALSRKVKIALAR
                     ASRLSGISENTGEGGMLDEQRAEADQLIFQCLSGRLGWNVKDMQRADAIEIYISQGAK
                     PGLGGQLMAKKVTPELAAIRGIPVGIDLRSPSRHPDVLGADDLVIKLQEFREATFHKV
                     PIGIKMGAGRVNDDIKIAYKDGFDFVELDGLQGSTGAASTEVLENVGIPTLAAVQEAV
                     DGLAEIDAKDDMDLVMMGGLKDGVDIVKMLALGADCTSVGTAAIIAGGCIACMQCHVG
                     TCPVGIATQDPEHEARYDLDRQAMNMHRYFESLRWQIAAITKALGYDDVHSVGRGDLV
                     ALTPEAADITGLPFKPEHKDTRRPLTGLELLDRKAG"
     gene            4867..>6449
                     /locus_tag="VIW27_12305"
     CDS             4867..>6449
                     /locus_tag="VIW27_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740500.1"
                     /translation="MKISDADYQFSITANADLEDDTADIHYVPAPCQVACPIGTDAPS
                     YIAYIWEGKNEEALEAITATNPFSAICGRVCDAPCEPACRRADSDGAIAIRNLKRYVL
                     EALGPGYALPPVEVSKNETVAIVGAGPAGLTAAHDLAEAGYEVHLHEASDKLGGMMFW
                     GIPRFRLPESALQQDIDRMMAHCPGIKIHLNSALGRDVSLDELKQRHDAVLLAIGAFV
                     GKPMGIASEAEPFVRDGVGFLYEVNGGARPTLPETVLVIGGGDVAMDACRVAKRLPGV
                     NNVKVVYRRDYEAMPARREELHGAMEEGIEIIYNRQPVAVVEGALRCVRTELGEPDED
                     GRRRPVNVEGSEHDIDCGLVIAAVGQKAECGELDQLGLMDWDRVRTRLDGMRTDDDQV
                     FAAGDGAFGGSTIVEAMYQGHRAAYYIKAFLQGNDNPLPYRTPYRTRRVSICNDPAWE
                     LNPRVEQQFHGLGEVPVEFAEIESTYTQAEAKFEAARCFRCDAETGSADYTVSSRESI
                     FAMSRISPQQVEIEASILQG"
BASE COUNT         1258 a         2009 c         2023 g         1159 t
ORIGIN      
        1 tgcttgttgt ccggcaattc gatgcggccg aggatcacgt cgcgcaccca ccacgcctgg
       61 gcatcgaaca tgttgaaggt ataccactgg tcctgcatgc cgaggtagaa cagcttcgga
      121 ttgtcgcacc aggccacgcc cttgtagagg tcggctgtcg ccagccggtt ggcggtacgc
      181 agcttgaggc cctcggccat gaatggaaag tggtgcaggt agccggtgca cagaatgatc
      241 gcgtcgacct cggcggtgct gccgtccttg aaggtcgcgg tattcttttc caccttctgc
      301 agcagcggca cctctttcca gttgtccggc cacttgtagc ccatcggtgc ggtgcgatgg
      361 gatacggtga ccgacttgca gccgtacttc cagcactgcg agccgatatc ctcggccgag
      421 taagaggtac cgatgatcag gatatccttg tccctgaatt ccatcgcgtc gcggaaatcg
      481 tgcgcgtgca gcacgcggcc gttgaaggtt tcgaaacctt cgaaataggg cacgttcggc
      541 gtcgagaagt gcccgctggc gacgatcaca tggtcgaatt cctcggtgta ctcgatgtct
      601 ttgtcgagat cgtgcgcggt gacccggaag ttgccgttgg cgtcgtcgta ggacacgtgg
      661 cgcaccgggg tattgaaacg gcaccattgg cgcacaccgg ccttcttgac gcggccctcg
      721 atgtagtcga acagtaccgc gcgcggcggg taggacgcga tcggtttacc gaaatgatcc
      781 tcgaaaaagt aatcggcgaa ctcgaggcct tccttggggc cgttcgacca caggtagcgg
      841 tacatgctgc catgcactgg ttcgccgtgc tcgtcgagac cggtacgcca ggtgtagcgc
      901 cacaggccgc cccagtccgc ctgcttttcg aagcagacga tctcgggaat atcggcgcct
      961 ttctcgtttg ccgactggaa tgctctcagt tgcgccagcc cgctcgggcc ggcaccgatc
     1021 actgctacac gctttgccat gctgttctcc aactcgttga ttataatatt atttattgta
     1081 agggataggg gttaaacctg cgctccaagg gtagggaatt agagcatcac ggtcaaccga
     1141 gggtgccatt tttattggta cagaactggt ccggcaaggg cggcccgcgc gcaatcgatt
     1201 tgcatttgcc agcgcactgg gttctgattg tcggcattcg aattcgatga taccagcaat
     1261 ggcggataaa gaggcggaga tcgccgatat cgaaacggcg atagagcagc acagcgtcga
     1321 agcgctggag atcgcgatcg gcaagcaggt acgcgcgtcg cgcaagcgcc tgcatctgac
     1381 cgtggcggcg ttggccaagc aggcgcaact gtctaccggc atgctgtcga agatcgagaa
     1441 cgggcacacc tcgccatcgc tggccacgct gacctcgctg gcgcgcgcgc tgcaggtgcc
     1501 ggtgacgtcg tttttccgcg gttacgagga acagcgcgac gtaaccttca tcaaggccgg
     1561 agacggcctg ccgatcgagc gccgcggatc cggcgccggt caccagtacc agttgctcgg
     1621 ccacaccatc ggcaagccct ataacatcga gccttacctg atcacgatcg acgaccagtc
     1681 cgaagtgttc ccggtgtttc agcacagcgg catggaattc atttacatgc tcgagggacg
     1741 cgtgatctac cgccacgcca accagaccta cacgctcgaa gcgggcgatt cgctgttttt
     1801 cgatgccgag gcgtcgcacg gccccgacga aattcagaag ttgccctgcc gttacctgtc
     1861 gatcatcgtt tcggagtcga ccacgagctg attttgtttc ctgataggaa acaaaatttc
     1921 atttcagttg acccgtatga cggcttcgcg taagcttccg cgatcgaatt caactccacc
     1981 ggagcaccga tatgtgtggt atcgcaggcg tcctgttcaa gcaccagggc gcgcacgaaa
     2041 tcggcaagac cctgatcgaa atgctcgacg gctgccagca ccgcggcccc gacagcactg
     2101 gcttcgcgct ctacggcgac aacaaggaat tgctgcaatt gcgcttcatc gtgccgaccg
     2161 aggacgccgc gcggcacgaa gcgatcgaca atatcgcgac cctgctcgag cgccacggcg
     2221 cgagcatcga cagcgaacag tcggtgggtt gctcctacca gatcgcggtg cgtttcgacg
     2281 gcgacctgct ggccttcacg aaagatatcg aggaacacgc caagctggtc tcggtcgggc
     2341 acacgctcga tatcatcaag gacgacggca ccgcgcacga actcgacgag ctataccacg
     2401 tcggcgagtt caggggcacc cacggtatcg gccacgtgcg tctggccacc gaatcggcgg
     2461 tgcggccgga ggccgcgcac ccgttctggg ccaccggttt cgaggatatc gccatcgtcc
     2521 acaacggtca gatcaccaat tactggaaga tgcgccgccg cctcgagcgc cgcggctata
     2581 gtttccatac cgacaacgat agcgagctgg tggcggtcta cctcgccgac aagctgtcgc
     2641 agggggaaac cctcgatcac gtgctggaga ccgcgatcgc ggatctcgac ggcacctttt
     2701 cgttcctggt ctcgaccagg gacgaaatcg gctacgccaa ggacaacctc gccgccaagc
     2761 cgatggtgct ctacgaaacc gacgacatga tcgccatcgc ctcggaagaa gtcagcctca
     2821 accgtttgtt cccggggcaa tccatcaatt ccaccgaacc cgcgccgggg acgcatcaga
     2881 catggtccag atcgatttaa gccgggtcgc gattaccgag gccaacgaca agattcgcgc
     2941 ctttgccgcg gaccgccagg aggttgaaat catcaacccg gatgcgcgcc acaacatcgg
     3001 cgtcggcctg gtcgtcgatg atccgatcac cgtccgggtc aggggatcgg ccggctattt
     3061 ctgtggcggc ctgacgcacg gggtgcgctt cgagatcgag cataacgcgg gctgggcggt
     3121 cggtgacaat atttaccagg gcaccgtcgt cgtcggcggc aacgccggcg cgatccccgg
     3181 ggtcgcgatt cgcggcgccg agatcgtcgt gcgcggtaat atgggcagcc gcgccggcca
     3241 ggtcatgaag gccggcacac tggcctgcgg cggcaacgcc gcgttcatgg ccggctacat
     3301 gatgtacggc ggacgcatca tcatcctcgg cgacgccgcg gccaaggtgg gccaggacat
     3361 gagtgccggt gaaatcttcg tcggcggcaa gatcgaaagc ctcggcaacg acacgatgat
     3421 cgtcgacatg gaggatcgcg agcgcgacga gatcatggaa ttcctcgatc gcttcgaact
     3481 gaaacaccac ggcgccttca ccaagatcgt caatgccggg aaaaagctgc gttacgcgaa
     3541 agccgaaccg cgccaccgcc cgcagccgtt tttcgtgtcg tcgaaatcgt ccaattactg
     3601 gaacgccaag gtgcaggaag atatctggat caagggtgag gtcgggcgtt accggattcg
     3661 cggctacggc gcgtccaagc cggtgccgca tctcaacgat attgccttcg tcaaggatgt
     3721 ctcgagcgtc gcccccgatc ccggggaact gaaggatatc aacctgaaga ccacggtcgg
     3781 cgggcgtttc ggcgccaaac cgatcagcct gtcgatgccg gtgatgatcg cgccgatgag
     3841 cttcggcgcg ctatcgcgca aggtcaagat cgcgctggcg cgcgcgtcgc gcttgtccgg
     3901 gatatccgag aacaccggcg agggcggcat gctcgacgag cagcgcgccg aggccgacca
     3961 gctgatcttt cagtgcctgt cggggcgtct cggctggaac gtcaaggaca tgcagcgagc
     4021 cgacgcgatc gagatctata tctcgcaggg cgccaagccc gggctcggcg gccagctgat
     4081 ggcgaagaaa gtcacccccg aactggccgc gatccgcggc atcccggtcg gcatcgacct
     4141 gcgttcgccg tcgcgccacc ccgacgtgct cggcgccgac gacctggtca tcaagctgca
     4201 ggaattccgc gaggcgacct ttcacaaggt gccgatcggt atcaagatgg gcgccggccg
     4261 cgtcaacgac gacatcaaga tcgcctacaa ggacggcttt gacttcgtcg agctcgacgg
     4321 cctgcagggt tcgaccggcg ccgccagcac cgaggtgctg gaaaacgtcg gcatcccgac
     4381 gctcgccgcg gtacaggaag cggtcgacgg gctcgccgaa atcgatgcca aggacgatat
     4441 ggacctggtg atgatgggcg ggctcaagga tggcgtcgac atcgtcaaga tgctcgcact
     4501 cggcgccgac tgtacctcgg tgggcaccgc ggcgatcatc gccggcggct gcatcgcctg
     4561 tatgcagtgc cacgtcggca cctgcccggt gggcatcgct acccaggacc cggaacacga
     4621 ggcgcgctac gacctcgatc ggcaggcgat gaacatgcac cgctacttcg aatcgctgcg
     4681 ctggcagatc gctgcaatca ccaaagcgct cggttacgat gacgttcaca gtgtcggccg
     4741 cggcgacctg gtcgcgctga ccccggaagc cgccgatatc accggcctgc cgttcaagcc
     4801 ggaacacaag gatactcgcc ggccgctgac cggcctcgaa ttgctcgatc gcaaggcggg
     4861 ttgacgatga agatttcgga cgccgattac cagttttcga tcaccgcgaa cgccgatctc
     4921 gaagacgata ccgcggatat ccattatgtt ccggcacctt gccaggtggc ctgcccgatc
     4981 ggcaccgacg cgccgtccta tatcgcctat atctgggaag gcaagaacga ggaagcactc
     5041 gaggcgatca ccgcgaccaa tccgttcagc gcgatctgcg ggcgcgtctg cgatgcacct
     5101 tgcgagcccg cctgccggcg cgccgacagc gatggtgcga tcgcgatacg caatctcaag
     5161 cgctacgtgc tggaggcgct cgggccgggt tacgccctgc cgccggtcga ggttagcaag
     5221 aacgagaccg tcgccatcgt tggcgcggga ccggcgggcc tgaccgcggc gcacgatctc
     5281 gccgaggcgg gttacgaagt gcacctgcac gaggccagcg acaagctcgg cggcatgatg
     5341 ttctggggta ttccgcgttt tcggttgccg gagtcggcgc tacagcagga catcgatcgc
     5401 atgatggcgc attgccccgg tatcaagatt cacctgaaca gtgcactcgg ccgcgacgtt
     5461 tcgctcgacg agctcaagca gcggcacgat gccgtgttgc tcgcgatcgg cgccttcgtc
     5521 ggcaagccga tggggattgc cagcgaagcc gaacccttcg tgcgtgacgg cgtcggcttt
     5581 ctctacgagg tcaacggcgg cgcgcgaccg acgctgccgg agaccgtgct ggtgatcggc
     5641 ggcggcgatg ttgcgatgga cgcctgtcgc gtggccaagc ggttgccggg cgtgaacaac
     5701 gtcaaggtcg tttaccgccg cgactacgag gcgatgccgg cgcggcgcga ggaattgcac
     5761 ggcgccatgg aagagggcat cgagattatc tataacaggc agccggtcgc ggtcgtcgaa
     5821 ggcgctctgc gctgcgtgcg caccgaactc ggcgagcccg acgaggacgg ccgccgccgg
     5881 ccggtcaatg tcgagggctc ggagcacgac atcgactgcg gcctggtgat tgccgcggtc
     5941 gggcagaaag ccgagtgcgg cgagctcgac caactcgggc tgatggactg ggaccgcgtg
     6001 cgcacgcgtc tcgacggtat gcgcaccgac gatgaccagg tattcgccgc cggcgacggc
     6061 gcctttggcg gttcgaccat cgtcgaagcc atgtaccagg gccatcgtgc cgcctattac
     6121 atcaaggctt ttttgcaggg caacgacaat ccgctgccct atcgcacgcc ataccgcacc
     6181 cgtcgcgtgt ctatttgcaa cgacccggcc tgggaactta acccgcgcgt cgagcagcaa
     6241 ttccacggcc tcggcgaggt gccggtcgag tttgccgaga tcgagtccac ctacacacag
     6301 gccgaagcca aattcgaggc cgcgcgctgt tttcgctgcg acgccgagac cggctcggcc
     6361 gactacacgg tcagcagccg cgaatcgatc ttcgcgatgt cgcgaatatc tccgcaacag
     6421 gtcgagatcg aggcgagtat tctgcaggg
//
LOCUS       DATCZV010000546         6139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_203769, whole genome shotgun sequence.
ACCESSION   DATCZV010000546 DATCZV010000000
VERSION     DATCZV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203769"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VIW27_12310"
     CDS             <1..261
                     /locus_tag="VIW27_12310"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5740501.1"
                     /translation="IELPDDSVQKTLDTLAGITPNSTTSMQRDLIRGRPSELDAQNGA
                     AVRLGHEVGIDTPVNRFFLYSLRSLELRARGALSFNRRSIHR"
     gene            complement(247..2418)
                     /locus_tag="VIW27_12315"
     CDS             complement(247..2418)
                     /locus_tag="VIW27_12315"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY5740502.1"
                     /translation="MSNRVEISGLSVDRDLYELIDKEIAPGTGVDSETFWQAFADIEA
                     ELGPENRRLLKFRDDLQAQIDAWHQSHGTTFDAAEYRAFLEGIGYLVPEGGEFKVTTA
                     NVDPEIGEISGPQLVVPVDNARYALNAANARWYSLYDALYGTDIILEVEGCKKTAKYN
                     PVRGQQVIRHVREFLDRAVPLAAGSHSYVTQYKVASGKLIGVMGDGSKAELGWRESFV
                     GYRGDPAKPEAILLQRNGLHIELLIGEGYYIGQGDLANVYDVRMESAITTIMDCEDSV
                     AAVDAEDKVRVYRNWNGLMKGDLKTTVRRGDETIERVLNPDLEYLSPQGETITLPGRS
                     MMFVRNVGTHMYTDAVTRDGEEVAETFLDAMVTVLAAKHDLLGNGKFQNSRTGSVYIV
                     KPKMHGPEEVAAAVQLFERVEDALGLESGTLKIGIMDEERRTTVNLKECIRAASDRVV
                     FINTGFLDRTGDEIHTSMEAGVMIPKNEMKNSRWLLAYEDWNVDIGLETGLPGHGQIG
                     KGMWAAPDNMAAMMEHKIAHPRAGATTAWVPSPVAACLHAMHYHQVNVSARQRQLASV
                     ARASLDDILTIPLLGDRGIKEGDLIRELENNAQGILGYVVRWIEQGVGCSKVPDISDV
                     NLMEDRATLRISSQHIANWLHHGITTPEQVRKVFEKMAQIVDRQNTGDPNYRDMAPDF
                     DDSIAFKGALDLVFRGREVANGYTEFVLHERRREFKQSAVD"
     gene            complement(2723..3613)
                     /locus_tag="VIW27_12320"
     CDS             complement(2723..3613)
                     /locus_tag="VIW27_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5740503.1"
                     /translation="MNSNHAKGLLITACGVLVISPDGLLTRLIAADHWTMIFYRALFL
                     AVGMWLVLGVTSRGRVWRQYQTLRGGALLKVLAYSFGTISFVFAITHTSVANTLVILA
                     TTPLFAAILSRLLLNEKIEARVLLAIGLVAAGIAVIAYGSGHGPGDRLGDMVALLGAF
                     FLACGFSFVRRFPDASSMAAISCSGLLTALLILPLAAPLSVSAADLGYLAIMGLAVVP
                     IGSAMMFLGPRYIPAPEVGLLMLLESLLGPLWVWLALGEQPGYHTLLGGAIVLVTLAL
                     NAAWALKNNRSRPAHLARRA"
     gene            3804..5480
                     /locus_tag="VIW27_12325"
     CDS             3804..5480
                     /locus_tag="VIW27_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019096239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5740504.1"
                     /translation="MSSNSQSRSGAELLIDALRSNAVERIFCIPGESYLAALDALYDR
                     GEISLVVCRNEGGAAYMAEADGKLTGRPGVCFVTRGPGACNASGGLHVAMQDSTPMIL
                     LIGQVARKDIEREAFQEIDYRRMFSQLAKWVAQIDDAERIPEYLNRAFSVATSGRQGP
                     VVLALPEDMLVDRSGAADAGAWVAVTASPAAEDVERAARMLAAAERPLIVVGGSGWSD
                     ALRVELQEFAAAWQIPVANSFRCQDYFDNEHSNYAGDLGLGVNPALTRRIAETDCLLV
                     IGARMGEMTTAGFSLIDIPRPRQRMIHVHPGAEELGHIYRPELAINAASQRFVAALAK
                     HAPPPGISDARRQRVEQAHRDYLDWNRSIRVDGELQLSDVVHSIRQQTPADTIVCNGA
                     GNNTGWLHRFFRYREYRTQLAPTSGTMGYGLPAAIAARLRYPGRTVVAFAGDGDFMMN
                     GQELATAMQYDAAIIVLVINNGTYATIRMHQEREYPGRVIGTDMVNPDFVALAAAHGA
                     HAERVTASDEFPAAFARCLDCGRLALIELHLDADILTPTTTVRGLRGAKN"
     gene            5534..5764
                     /locus_tag="VIW27_12330"
     CDS             5534..5764
                     /locus_tag="VIW27_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HEY5740505.1"
                     /translation="MPIAIDQLLIQSLQLLGIGMGAVFVILCSLIVLISIVARLVPEE
                     APPVVKQDAPKIDDSHIAAITAAVHQFRKNRK"
     gene            5766..>6139
                     /locus_tag="VIW27_12335"
     CDS             5766..>6139
                     /locus_tag="VIW27_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005593991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEY5740506.1"
                     /translation="MANPLLITDLVLRDASQSLLATRVRIEDMLPIAAKLDRVGFWSL
                     ETWGGATFDACIRYLGEDPWERLRQLKAAMPNTPMQMLLRGQNALGYRHYADDVIEKF
                     VERCAANGMDVFRIFDAMNDMR"
BASE COUNT         1105 a         1994 c         1929 g         1111 t
ORIGIN      
        1 atcgagctgc ccgatgacag cgtgcagaag accctcgaca cgctggccgg catcacgccc
       61 aactcgacca cctcgatgca gcgtgacctg attcgcgggc gaccatcgga actcgatgcc
      121 cagaacggcg ccgcggtgcg cctggggcac gaggtcggga tcgacacacc ggtcaatcgc
      181 ttctttttgt acagcctgcg ctcgctggag ctgcgcgcgc gcggcgcgct gagtttcaac
      241 cgccgctcaa tccaccgctg attgcttgaa ttcccggcgc cgctcgtgga gcacgaactc
      301 ggtgtagccg ttcgccacct cgcggccgcg gaacaccagg tcgagggcgc ccttgaaggc
      361 gatgctgtcg tcgaagtcgg gggccatgtc gcggtaattc gggtcgccgg tattctggcg
      421 atcgacgatt tgggccatct tttcgaacac cttgcgtacc tgttccggcg tggtgatgcc
      481 gtggtgcagc cagttggcga tatgctgact cgagatgcgc agcgtggcac ggtcctccat
      541 caggttgacg tcggagatat ccggcacctt tgagcagccc acgccctgct cgatccagcg
      601 caccacgtag ccgagaatac cctgcgcatt gttttccagt tcgcgaatca ggtcgccttc
      661 cttgatcccg cgatcgccga gcagcggaat ggtcaggatg tcgtcgaggc tggcgcgcgc
      721 gacgcttgcc agttgacgct ggcgggcact gacgttcacc tgatggtaat gcatcgcgtg
      781 caggcaggcg gcgaccggcg acggcaccca ggcggtcgtc gcgccggcgc gcggatgggc
      841 tattttgtgc tccatcatcg ccgccatgtt gtcgggcgcg gcccacatgc ccttgccgat
      901 ctggccgtgc ccgggcaacc cggtctcgag accgatgtcg acattccagt cctcgtaggc
      961 cagcagccag cgtgaattct tcatctcgtt cttggggatc atcacgccgg cttccatgct
     1021 ggtgtggatt tcatcgccgg tacgatcgag gaaaccggta ttgatgaaga ccacgcgatc
     1081 gctggccgcg cgaatgcatt ccttgaggtt gaccgtggta cggcgctctt cgtccatgat
     1141 gccgatcttc agcgtgccgg actcgagacc gagcgcgtcc tcgacgcgct cgaacagctg
     1201 caccgcggcc gcgacttcct cgggaccatg catttttggc ttgacgatat agacgctgcc
     1261 ggtgcggctg ttctggaact tgccgttgcc gagcaggtcg tgcttggcgg cgagcacggt
     1321 gaccatcgca tcgagaaaag tttccgccac ttcctcgccg tcgcgggtca cggcatcggt
     1381 atacatgtgc gtgccgacgt tgcgtacgaa catcatgctg cgccccggca gggtgatcgt
     1441 ttctccttgt ggcgacagat attcgaggtc cggattgagg acccgctcga tggtttcgtc
     1501 gccgcgcctg acagtggttt tcagatcgcc tttcatcagg ccgttccagt tgcggtagac
     1561 gcgcaccttg tcctcggcgt cgacggcagc caccgagtcc tcgcaatcca tgatcgtggt
     1621 gatcgccgat tccatgcgca cgtcgtagac gttggcgaga tcgccctggc cgatgtaata
     1681 gccttcgccg atcagcagct cgatgtgcag gccgttgcgc tgcagcagta tggcctcggg
     1741 ttttgccgga tcgccacggt aaccgacgaa gctttcgcgc cagccgagct cggccttgct
     1801 gccgtcaccc atcaccccga tcaacttgcc ggaggcgacc ttgtactgcg tcacgtagct
     1861 gtggctgccg gccgccagcg gcaccgcccg gtcgagaaat tcgcgcacgt gacgaatcac
     1921 ctgctgaccg cgcaccgggt tgtatttcgc ggtcttcttg cagccctcga cctcgagaat
     1981 gatatcggtg ccgtacagcg cgtcgtagag gctgtaccag cgggcattgg ccgcgttcag
     2041 cgcgtaacgc gcgttgtcca ccggcaccac cagctgcggc ccggaaatct caccgatctc
     2101 ggggtccacg tttgcggtgg tgaccttgaa ctcgccgcct tcgggaacca ggtagccgat
     2161 tccctcgaga aaggcccggt attcggcagc atcgaaagtt gtgccatggc tttgatgcca
     2221 ggcgtcgatt tgcgcctgca gatcgtcgcg aaatttcagc aggcgccgat tttcgggacc
     2281 cagctcggcc tcgatatcag cgaacgcctg ccagaaggtc tcgctgtcga ccccggttcc
     2341 gggagcgatt tccttgtcga tcaattcata caggtcgcgg tcgacactga gaccgctgat
     2401 ttcaactcgg ttgctcatcg attcattcca gttttgattc atgcacggcc tagccccgcg
     2461 caacagccct gcagtatatc cgcccgcttg gcgaaaacca gccatcaaag cacattattg
     2521 gaaagcttta tatatagtct taacccgcct gttacatgcg tactccgact gccagcgcgg
     2581 gaaaactgtc gccgggcgcc gcgatagaac gagcgccgat tagccagtca gtcgcggcgt
     2641 tctcgattca gttgccaaaa atttccgctt ccgactcgat agtatatcga cagctgcgga
     2701 ttcggctcat gcgataggcg gtttacgccc tgcgcgccag gtgcgccggc cgcgagcgat
     2761 tgtttttcag cgcccaggcg gcgttgagcg ccagcgtcac cagtacgatc gcgccgccga
     2821 gcagcgtgtg gtacccgggc tgctcgccga gcgccagcca gacccagagc gggccgagca
     2881 gcgattccag cagcatcagc aacccgacct cgggcgccgg gatgtaacgc ggcccgagga
     2941 acatcatcgc cgaaccgatc ggcaccaccg cgagacccat gatcgccagg taaccgagat
     3001 cggccgccga caccgacagc ggcgcggcca gcggcaatat caatagcgcg gtcagcaggc
     3061 cgctgcagga tatcgctgcc atgctcgagg catccgggaa gcggcgcacg aagctgaaac
     3121 cgcaggcgag gaaaaatgca ccgagcaggg cgaccatgtc gcccagccgg tcgccgggtc
     3181 cgtgcccgct gccataggcg atgacggcga taccggcggc gaccaggccg atggcgagca
     3241 acacgcgcgc ttcgattttt tcgttcagca gcaggcggct gaggatggcc gcgaacagcg
     3301 gcgtggtggc cagaatcacc agcgtgttgg cgacgctggt atgggtaatc gcgaacacga
     3361 aggaaatggt gccgaacgag taggccagca ccttcagcag cgcgccgcca cgcagggttt
     3421 gatactgccg ccagacgcgg ccgcggctgg tgacgccgag caccagccac ataccgaccg
     3481 ccagaaacag cgcccgataa aagatcatgg tccagtgatc ggccgcgatc agccgtgtca
     3541 gcaggccgtc cggcgagatc accagcaccc cgcaggcggt tatcaacagg ccttttgcat
     3601 gattcgaatt caccgcgagg attttagagg taatggccgc gcttggcccg tgttttcggc
     3661 cgcgccgaat cggcatgcac aagcattgct ttttacgacg atcatggcga agatagccgc
     3721 gaactgtcgc cggcgacggc gccgcgaagc ggtccgcgag ggacagcagc cgggcctttt
     3781 catcaatccg caggcgagcc atcttgagca gtaacagcca gtcgcgcagc ggcgccgagc
     3841 tcctgatcga cgcactccgc agcaacgcgg tggagcgcat cttttgtatt ccgggggaaa
     3901 gttacctggc ggcgctggac gcgctctacg accgcggcga aatctcgctg gtggtgtgcc
     3961 gcaacgaggg cggcgcggcc tacatggccg aggccgacgg caagttgacg ggccggccgg
     4021 gcgtctgttt cgtgacccgc ggcccgggcg cctgcaatgc cagcggcggc ctgcacgtgg
     4081 cgatgcagga ttcgacgccg atgatcctgc tgatcggcca ggtggcgcgc aaggacatcg
     4141 agcgcgaagc ctttcaggaa atcgattacc ggcgcatgtt ttcgcaactc gccaagtggg
     4201 tcgcgcaaat cgacgacgcc gagcgcattc ccgagtacct caaccgcgct ttcagcgtcg
     4261 ccaccagcgg ccgccagggc ccggtagtgc tggcgctgcc cgaggatatg ctggtcgacc
     4321 gcagcggcgc tgccgatgcc ggcgcgtggg ttgcggtgac cgccagcccg gccgccgagg
     4381 acgtcgagcg cgcggcgcga atgctcgccg ccgccgagcg gccgctgatc gtggtcggcg
     4441 gcagcggctg gagcgacgcg ctgcgtgtcg agctgcagga gttcgcggcg gcctggcaga
     4501 tcccggtggc gaactcgttc cgctgccagg attacttcga caacgagcac tcgaattacg
     4561 ccggcgatct cgggctcggc gtcaacccgg cgctgacccg gcgcatcgcc gaaaccgatt
     4621 gcctgctggt catcggtgcg cgcatgggcg agatgaccac cgccggtttc agcctgatcg
     4681 atattccccg cccgcgtcag cgaatgatcc acgtgcaccc cggcgccgag gaactcggcc
     4741 acatctaccg gccggagctg gcgatcaacg ccgccagcca gcgattcgtc gcggcgttgg
     4801 cgaaacacgc gccgccgccc gggatcagcg atgcccgccg gcaacgggtc gagcaggcgc
     4861 atcgggatta cctcgactgg aaccgcagca ttcgcgtcga cggcgagctg cagctgtcgg
     4921 acgtggtcca cagcattcgc cagcaaacgc ccgccgacac catcgtttgc aacggcgccg
     4981 gcaacaatac cggctggctg caccgctttt ttcgctaccg cgaataccgc acccagctgg
     5041 ccccgaccag cggcaccatg ggctacggcc tgccggcggc gatcgccgcc cggctgcgtt
     5101 accccgggcg caccgtggtg gcgttcgccg gcgacggcga cttcatgatg aacggccagg
     5161 agctggcgac cgcgatgcag tacgacgcgg cgatcatcgt gctggtgatc aacaacggca
     5221 cctacgcgac cattcgtatg caccaggaac gcgagtatcc gggccgcgtc atcggcaccg
     5281 atatggtcaa ccccgatttc gtcgctctcg ccgcggccca cggcgcgcat gccgagcggg
     5341 tgacggcaag cgacgaattc ccggcggcct tcgcgcgctg cctggactgc ggccggctgg
     5401 cgctgatcga gctgcacctc gatgccgata tcctgacgcc gacaacgacc gtgcgcggct
     5461 tgcgcggcgc gaaaaactga attttaccgc tttcgatagt atgattagcg ctctttccgc
     5521 ataagccgaa cgcatgccga tagccatcga tcaactattg attcaaagcc tgcagctgct
     5581 gggtatcggc atgggcgcgg tgttcgtcat cctctgctcg ttgatcgtgc tgatatcgat
     5641 cgttgcccgg ctggtgcccg aagaagcccc gccggtggtc aaacaggacg cgccgaagat
     5701 cgacgacagc cacatcgcgg caatcactgc ggccgttcat caattccgaa aaaacaggaa
     5761 ataacatggc gaatcccctg ttgattaccg atctggtgct gcgcgacgcg tcgcaatcgc
     5821 tgctggcgac gcgcgtgcgt atcgaggaca tgctgccgat cgcggcaaag ctcgaccggg
     5881 tcggcttctg gtcgctggaa acctggggcg gcgcgacctt cgacgcctgc atccgctacc
     5941 tcggcgaaga tccctgggaa cgcctgcgcc agctcaaggc ggcgatgccc aacacgccga
     6001 tgcagatgct gctgcgcggg cagaatgcgc tcggttaccg ccactacgcc gacgacgtga
     6061 tcgagaaatt cgtcgagcgc tgcgccgcca acggcatgga cgtgttccgt atcttcgacg
     6121 cgatgaacga catgcgcaa
//
LOCUS       DATCZV010000547         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_520595, whole genome shotgun sequence.
ACCESSION   DATCZV010000547 DATCZV010000000
VERSION     DATCZV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520595"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1287)
                     /locus_tag="VIW27_12340"
     CDS             complement(19..1287)
                     /locus_tag="VIW27_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011047304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEY5740507.1"
                     /translation="MSQQANVKYLSEEDLEMFPKLEIPQTLAAGPGPGNTDPRVLEAY
                     ATAGCADHMQADVVRGMKEAKLMLREVFGTSNVYTFAVCGTGWDGLDCVFSPILPGDT
                     VVSFVNGTFSGIDDFNIRLKASTPEDLAKNPLDPQPANVVTVNVPHGQSVTGEIVEAA
                     LAEHKPMWAFMAHWETGSGRINDIQGFNDACEKHGVMGIVDAVSSLGISYFNIDDYPA
                     VASWASCPQKGILGLPLTYAPVSFNDKVIELLSKRGCYTYVHHPILEARHWCIADGKD
                     VDVAAYHRTHSGYAVASFHEALRLLLQHGRAQKSNDYAFYEMGLRTAIEAMGCKVTSN
                     MTSLVVFDLPAAFEGREKELVQGCRAEGFAIWNTLSEPAQIRIGILNQLTIPALNDIV
                     GRFADAMIKLGAEVDKAAILSDLNDYLASR"
     gene            complement(1313..1711)
                     /locus_tag="VIW27_12345"
     CDS             complement(1313..1711)
                     /locus_tag="VIW27_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY5740508.1"
                     /translation="MRQKHAIDLDTARKIAAAAEYEAQHHALQVVIAVVDDGGHLLYL
                     QRDGAQPGSVEVAIGKARSALLFRRPTRAFEELVESGRQGYLAMPGVMPLEGGEPLVY
                     RDEIVGAIGVSGASSTDDGVVARAGAAAID"
     gene            complement(1731..2573)
                     /locus_tag="VIW27_12350"
     CDS             complement(1731..2573)
                     /locus_tag="VIW27_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY5740509.1"
                     /translation="MNNAVTNMTRKRGRPPGRGNRAGEGRNQSLTRALTLLQRLAEAP
                     QGMQLTDLSYQLGMPVATVHRLLATFEELDFVEQDSEQGRWYVGLNAFTVGNAFLKRR
                     DFVAIARPRMDALVEQCGETVNLGVIDDGQVVFVSQVESQEVMRMIVRLGSRAPIHAS
                     GVGKALLAGMTAQRVERILERRGLPRFTERTIGNPADLHAELELTRRRGYALDDEEHA
                     VGLRCVAAAVFDENARALAAISLSGPKARMVDERLGELGQAVRGTAESITLALGGRKP
                     DGLV"
     gene            2920..>3636
                     /locus_tag="VIW27_12355"
     CDS             2920..>3636
                     /locus_tag="VIW27_12355"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY5740510.1"
                     /translation="MRIAVYGTGGVGGYFGGRLAQIGQDVTFIARNENLAALRSSGLR
                     VGSISGDFVIDRVKATNNPAEVGVVDYVLCCVKAWQLPAAAKAIKPMVGADTLVIPLQ
                     NGVEAPDQLSVELDPQNVLGGLCAIVAFQSGPGHIKHSGANPLIRFGHLDNHADPRVN
                     ALSEIFNHCSGVKSSIPDDILVALWLKFMLITPWSGLGAVSRAPIGVLMEQPETRELL
                     VEAAEEVYRVGIARGIELPDD"
BASE COUNT          664 a         1137 c         1126 g          709 t
ORIGIN      
        1 acgacggaga gaagccgtct aacgactcgc gaggtagtcg ttcagatcgg agaggatcgc
       61 ggccttgtcg acctcggcgc cgagtttgat cattgcgtcg gcgaatcggc cgacgatatc
      121 gttgagtgcc ggaatggtca gttggttgag aatgccgata cgaatctgcg ccggctccga
      181 cagcgtgttc cagatcgcga agccctcggc gcggcaaccc tgcaccagct ccttctcgcg
      241 gccttcgaaa gccgccggca aatcgaacac caccaggctg gtcatgttcg aggtcacctt
      301 gcagcccatc gcctcgatcg cggtacgcag gcccatctcg tagaacgcgt aatcgttcga
      361 cttttgcgcc cggccatgct gcagcagcag gcgcagggct tcgtggaacg aggccaccgc
      421 gtaaccggag tgggtacggt gatatgccgc gacatcgaca tccttgccgt cggcgataca
      481 ccagtgacgc gcctcgagaa tcgggtgatg cacgtaggta tagcaaccac gcttgctgag
      541 cagttcgatg accttgtcat tgaagctcac cggcgcataa gtcagcggca gaccgaggat
      601 gcccttttgc gggcacgagg cccagctggc aaccgccggg taatcgtcga tgttgaaata
      661 ggagatgccg agactcgaaa ccgcgtcgac gatacccatt acgccgtgct tttcgcaggc
      721 gtcgttgaaa ccctgaatat cgttgatgcg gccgctgccg gtttcccagt gcgccatgaa
      781 cgcccacatc ggcttgtgct cggcgagcgc ggcttcgacg atctcgccgg tcaccgactg
      841 gccgtggggc acgttgaccg taaccacgtt ggccggctgc gggtcgagcg ggtttttcgc
      901 caggtcctcg ggcgtcgacg ccttcaggcg gatattgaaa tcgtcgatgc cgctgaaggt
      961 gccattgacg aagctgacca cggtgtcgcc cggcaggatc ggcgagaata cgcagtcgag
     1021 accgtcccag ccggtgccgc agaccgcaaa ggtgtagacg ttgctggtgc cgaacacttc
     1081 gcgcagcatc agcttggctt ccttcatgcc gcgtaccacg tcggcctgca tgtggtcggc
     1141 gcaaccggcc gtggcatagg cctcgaggac ccgcggatcg gtgttgcccg gcccgggacc
     1201 ggccgccagg gtttgcggaa tttccagctt gggaaacatt tcgaggtcct cttcggacag
     1261 gtatttcacg tttgcttgtt gagacatgga ttgtttactc ccgatttaaa attcagtcaa
     1321 tggccgccgc gccggctcgc gcgacgacac cgtcatctgt tgaactcgca ccgctgacgc
     1381 cgatggcgcc gacgatctcg tcccggtata cgagtggttc gccgccctcg agcggcatca
     1441 cgcccggcat cgccaggtag ccctgccgtc cgctttccac caattcttcg aacgcccgcg
     1501 tcggccgccg gaacagcagt gccgagcgcg ccttgccgat cgccacctcg acgctgccgg
     1561 gctgcgcgcc atcgcgctgc aggtacagca ggtggccgcc gtcgtcgacg accgcgatca
     1621 ctacctgcag ggcgtggtgc tgggcctcgt actcggccgc cgcggcaatc ttgcgcgcgg
     1681 tgtcgagatc gattgcatgc ttttgccgca tattcgattc cccgctagtc tcagaccagg
     1741 ccgtcgggct tgcgcccgcc gagcgccagg gtgatcgact cggcggtgcc gcgcaccgct
     1801 tgcccgagct cgccgagccg ctcgtcgacc atgcgcgcct tgggcccgga cagcgagatc
     1861 gccgccagcg cacgcgcgtt ttcgtcgaaa accgccgccg ccacgcaacg caggccgacc
     1921 gcgtgctctt cgtcgtcgag cgcgtaaccg cgccggcggg tgagttcgag ttcggcgtgc
     1981 agatcggcgg ggttgccgat ggtgcgctcg gtaaagcgcg gcagcccgcg gcgctcgagt
     2041 attcgctcga cgcgttgcgc ggtcatgccg gccagcagcg ccttgccgac accggaagcg
     2101 tggatcggcg cgcggctgcc gaggcgcacg atcatgcgca tgacttcctg cgactcgacc
     2161 tggctgacga agaccacctg gccgtcgtcg atgacgccga gattgacggt ctcgccgcac
     2221 tgctcgacca gcgcatccat tcgcggcctg gcgatggcga cgaagtcgcg ccgcttgagg
     2281 aaggcgttgc cgaccgtgaa tgcgttcagc ccgacgtacc agcgtccctg ctcgctgtcc
     2341 tgctcgacga aatcgagttc ctcgaaggtc gccagcaagc ggtgcaccgt cgccactggc
     2401 atgcccagct ggtaggacag gtcggtcagc tgcatgccct gcggcgcctc ggcgaggcgc
     2461 tgcagcagcg tcagcgcgcg cgtcagcgac tgattgcggc cctcgccggc gcgattgccg
     2521 cgccccggcg ggcggccacg cttgcgggtc atgttggtca cggcattgtt catggcgcgc
     2581 aatctatcag ccacgagcct tcgaaacatg cctggaaacg acaggtatca tttaaaaatt
     2641 aattccaaaa aaaatttaat ctatctataa aaatttctat attactgaaa ttcaatgaaa
     2701 tttcttggat ttcactatta ttattatttt tggaaattat tatcatataa tgaaaaacca
     2761 gagtttagcc gaaaaatcgc ggctctgcaa tcgccgctta ggggagcctt gttccgcacc
     2821 ggcttggaat tgcgtgacgc cgttccggga tttttttaaa caaatttccg agactgcgac
     2881 ataatagtga tcaatccatg ccttgttaga ggagtcagaa tgcgcattgc ggtatacgga
     2941 acgggtggcg tcggcggcta tttcggcgga cggctggcgc agatcggcca ggacgtgact
     3001 ttcatcgcgc gcaacgagaa cctggcggcc ctgcgctcat cgggtctgcg ggtcggcagc
     3061 atttccggtg atttcgtcat cgacagggtc aaggcgacca acaacccggc cgaggtcggg
     3121 gtcgtcgact acgtgctgtg ctgcgtcaag gcctggcaat tgccggccgc cgcgaaagcg
     3181 atcaagccga tggtcggtgc cgacacgctg gtgataccgt tgcagaacgg cgtcgaggcg
     3241 cccgaccagt tgagtgtcga gctcgacccg caaaacgtcc tcggtgggct gtgcgcgatc
     3301 gtcgccttcc agtccgggcc cggtcatatc aagcacagcg gcgccaatcc gttgattcgc
     3361 ttcggccatc tcgacaacca tgccgacccg cgagtcaacg cgctatccga aatcttcaat
     3421 cactgcagcg gcgtcaaatc gagcattccc gacgacatcc tggtcgcact gtggctgaag
     3481 ttcatgctga tcacgccgtg gagcggactc ggcgcggttt cgcgcgcacc catcggcgtc
     3541 ctgatggagc aaccggaaac gcgcgaattg ctggtcgagg cggccgagga agtctatcgc
     3601 gtgggcatcg cgcgcggcat cgagctgccc gatgac
//
LOCUS       DATCZV010000548         5242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_786488, whole genome shotgun sequence.
ACCESSION   DATCZV010000548 DATCZV010000000
VERSION     DATCZV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786488"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1095
                     /locus_tag="VIW27_12360"
     CDS             253..1095
                     /locus_tag="VIW27_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5740511.1"
                     /translation="MKKQEIVAIFRKRLLESMHERGVNQSSLSRETGVDRSTLSQLLA
                     EENLRMPRADTVASLARALQVSSDWLLGLSQDSRFGAEILRQSFEVAPQEPSPADDNL
                     KRWYEEAAGLKIRYVPTLLPDIFKTDRVLEYEYAEVMERPVSRAKEQAQQTRNFSRGP
                     DNDMEICMSSQALEDFCRGESIWRNLDPGARAEQVETLREMITELYPSVRLYLFDGRK
                     RYSVPYTIFGARRAAVYMGQMYFAFNTTDYVRILVRQFDDLVRNASVQAHEVADFVAA
                     RAAG"
     gene            1286..2596
                     /locus_tag="VIW27_12365"
     CDS             1286..2596
                     /locus_tag="VIW27_12365"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008889 - glycerophosphodiester
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY5740512.1"
                     /translation="MQPSLTGLISGLAIAALLSAGAAIARDRNKQPDEFPELEALLLD
                     LGNKGKGRIESQLRPNFLVDDMGSGKLKDKLASCSGTKLRSSEFSIGHRGAPLQYPEH
                     TLESYVAAARQGAGIVECDVTFTRDRELVCRHSQCDLHTTTNILQIPRLAAKCSQPFT
                     PYDPATGTPASARCCTSDITLQEFKSLQGKMDAANPRGTTVDEYLSEDASATWRTDLY
                     SGRGTLLTHAESIWLFRKLGVGMTPELKAPSVAMPFEGDYTQQDYAQQMIDEYKQAGV
                     EPKSVWAQSFKLEDIRYWIENEPRFGKQAVYLDSRVYDDSGFEPTLADFEALKRSGVE
                     IVAPPMFALLTLDAHGDIVPSAYAELARAAGLEIITWTFERTDLRDGAQAGEFYYQTI
                     ADAITRDGDRYLVLDALARKVKVRGIFSDWPASVTYYANCMGLK"
     gene            complement(2663..3073)
                     /locus_tag="VIW27_12370"
     CDS             complement(2663..3073)
                     /locus_tag="VIW27_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012325371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5740513.1"
                     /translation="MSRKNIIRVRDVMKTGFDMVEGMDTVADALRNSKYPETKCFIVN
                     MRHDDDEYGIVLLSDIAKQVLARNKAPERVNIYEIMSKPVIDIHPDMDIRYCARMFEN
                     FGLARAPVIDGRQVIGVVGYTDIVLSGVRDKLLR"
     gene            complement(3070..3420)
                     /locus_tag="VIW27_12375"
     CDS             complement(3070..3420)
                     /locus_tag="VIW27_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5740514.1"
                     /translation="MHFKLIIALTQDSITDRIVEAARGKGATGATVIPSARGEGLETA
                     KTFLGLSLETQRDVILMLVEEHLCRDILETIEHAGDFDESPGTGIAFSIDVEDAVGVS
                     HQVSQLSRLIEEEI"
     gene            complement(3433..4212)
                     /locus_tag="VIW27_12380"
     CDS             complement(3433..4212)
                     /locus_tag="VIW27_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEY5740515.1"
                     /translation="MLEIFQDLLRTIVYTVQDVLPIAAVVFGFQVFILKKPIPHLARI
                     VKGFVLVLIGLGFFLEGLQSALFPLGRLMAQQLTDPAFIAAGSSGEIADWWDYHWVYL
                     FAFAIGFSTTVAEPSLLAVAIKATQVSAGAIDTWGLRIAVAIGVAIGIALGVFRIITG
                     TPLYWYIITGYLFVLLQTLLAPRMIIALAYDSGGVTTSTVTVPLVAALGLGLAATVPG
                     RNPLLDGFGLIAFASLFPIMSVLAYAQLSEWRGRRKQNRYS"
     gene            complement(4218..4949)
                     /locus_tag="VIW27_12385"
     CDS             complement(4218..4949)
                     /locus_tag="VIW27_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEY5740516.1"
                     /translation="MKSLKGIAAALLDSLRDLLPIIVVIAFFQLAVLRQPIPDFGEIV
                     VGIAFVVIGLTLFVQGLKLGLFPIGETMAFSFASRGNLFWLLCFAFALGFGTTVAEPA
                     LIAVAGEAALVAAQGGMIEHSESSLASYAFGLRLTVALAVGFAIVVGVFRIVMGWPIQ
                     YLIIVGYIGVVAMTAFAPHEIIGIAYDSGGVTTSTITVPLVTALGVGLASSIKGRNPM
                     VDGFGLIAFASLTPMIFVMGYGMLV"
     gene            complement(5069..>5242)
                     /locus_tag="VIW27_12390"
     CDS             complement(5069..>5242)
                     /locus_tag="VIW27_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020039506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5740517.1"
                     /translation="KVVEQGPKDEMFTPPHHPYTDLLLSSVPEMDPDWLTTLLEERGV
                     DDIGEAATRKMEE"
BASE COUNT         1113 a         1565 c         1586 g          978 t
ORIGIN      
        1 caggacgatc cagccggcgt aggtcgcgat cagcagcagc caggttggcc attccaggtc
       61 tgcagcgtcg aaagtggttt cgcggcggct ggcggatgtc atctacgcag ggtctccctg
      121 ggaaatttgc ggtatctgca atctaagttg ccaccgatgt tttgtctacg tgtttattgt
      181 aagcgttaag aaaattatga tttctaaaaa ataactctgt agtatttacg aaacaaattg
      241 tgctacaaaa tcatgaaaaa acaggaaatt gtcgccattt tccgcaagcg cctgctggaa
      301 tcgatgcatg aacggggcgt caatcaatcg tcgctatcgc gcgaaaccgg tgtcgatcgc
      361 tcgacgctgt cgcagttgct cgccgaggaa aacctgcgca tgccgcgcgc cgataccgtc
      421 gcctcgctgg ctcgcgcgct gcaggtcagc agcgactggt tgctggggtt gagccaggat
      481 agtcgtttcg gtgccgaaat cctgcgccag tccttcgaag tcgcgccgca ggaaccgagt
      541 cccgcggacg acaacctgaa gcgctggtac gaagaggcgg ccggcctcaa gatccgctac
      601 gtaccgaccc tgttgccgga tatattcaag accgaccggg tgctcgaata cgagtacgcc
      661 gaggtaatgg agcgcccggt gagccgcgcc aaggagcagg cgcagcagac ccgcaatttt
      721 tcgcgcggtc ccgataacga catggaaatt tgcatgtcca gccaggcgct ggaagatttc
      781 tgtcgcgggg agagtatctg gcgcaacctc gatccgggcg ctcgcgccga gcaggtcgaa
      841 actcttcgcg aaatgatcac ggaactctat cccagtgtgc ggctgtatct gttcgacggt
      901 cgcaaacgct attcggtccc gtatacgata ttcggtgcac gccgcgcagc ggtctacatg
      961 ggacagatgt atttcgcctt taatacaacc gattacgtgc gtatcctggt gcgccagttc
     1021 gacgacctgg tacgcaacgc ctcggtgcaa gcgcacgagg tcgccgactt tgtcgccgcg
     1081 cgtgccgccg gctgaacctc cgcttgcgcg acacggcagg gccatcgccc gttttgcatg
     1141 acatgttcgc tgtatattaa attggacggc aatctccatg cgaaattcac tctaccgtaa
     1201 tcctgccggc gaccttcttg tatagcatgg catgcggata cttcgtatca ctcgataccg
     1261 agccgacaac caggcaggag gaagcatgca gccttcgtta acgggtttaa tcagcggcct
     1321 ggcgattgcc gcattgctgt ccgcgggtgc cgcgatcgcc agggatcgca acaagcagcc
     1381 cgacgaattc ccggaactcg aagcgctgtt gctcgatctc ggcaacaagg gtaaaggccg
     1441 catcgagtcg cagctgaggc cgaatttcct cgtcgacgac atgggcagcg gcaagctgaa
     1501 ggacaagctc gcctcctgca gcggcacaaa gctgcgttct agcgagtttt ccatcggcca
     1561 tcggggagcg ccattacagt atccggagca caccctggaa tcctacgtcg ccgccgcgcg
     1621 ccagggcgcc ggcatcgttg agtgcgacgt gacctttacc agggatcgtg agctggtttg
     1681 ccgccattcg caatgcgacc tgcacacgac gaccaacatc ctgcagattc cgcggctcgc
     1741 ggccaaatgc agccagccgt tcacgcccta cgacccggcg accggaacgc cggcgtcggc
     1801 gcgatgctgc accagcgata tcacgctgca ggagttcaag tcgctgcagg gtaaaatgga
     1861 cgccgccaat ccgcggggga cgacggtcga cgagtacctg tccgaagacg cgagcgccac
     1921 ctggcgtacc gatctgtaca gcggtcgcgg caccctgctg acgcatgccg aaagcatctg
     1981 gctgttcagg aaactcggtg tgggcatgac gccggaattg aaggcgccga gcgttgccat
     2041 gcccttcgag ggtgattaca cccagcagga ttacgcccag cagatgatcg acgaatacaa
     2101 gcaagccggc gtcgagccga aaagcgtctg ggcgcaatcc ttcaagctcg aagatatcag
     2161 gtactggatc gaaaacgagc cgcggttcgg caagcaggcg gtgtatctcg acagccgcgt
     2221 ctacgacgac tccgggttcg aaccgacgct ggcggatttc gaggcgctca agcgcagcgg
     2281 cgtcgagatc gtcgcgccgc cgatgttcgc gctgctgaca ctcgacgcac atggagatat
     2341 cgtgccgtcg gcatacgccg agctggccag ggccgccggc ctcgaaatca tcacctggac
     2401 tttcgagcgt accgacctgc gcgacggggc acaggccggc gagttttatt accagacgat
     2461 tgccgacgcc atcactcgtg acggcgaccg ctacctggtg ctcgacgcgc tcgccagaaa
     2521 agtcaaagtc cgcggaatat tttccgactg gccggcgtcg gtcacctact atgcgaactg
     2581 catgggtttg aaatagcccg cactgccggg attcagccga catgattcct gcgcggccgc
     2641 agccgctgcg gggcttgccg ggttaccgaa gcaacttgtc gcgcactccg ctgagcacga
     2701 tatcggtata gccgacgacg ccgataacct ggcggccgtc gatcaccggg gcgcgggcga
     2761 gaccgaagtt ctcgaacatg cgcgcacagt aacggatatc catgtccggg tgaatgtcga
     2821 tgaccggttt ggacatgatt tcgtagatgt ttacccgctc cggagcctta ttgcgcgcaa
     2881 gtacctgctt ggcgatatcg gataacaaca cgattccgta ctcgtcgtcg tcgtggcgca
     2941 tattgacgat aaaacacttg gtttcgggat atttcgaatt gcgcagcgcg tcggcgacgg
     3001 tatccatgcc ttcgaccatg tcgaacccgg tcttcatgac atcgcgcacg cggatgatgt
     3061 ttttcctgct catatttcct cctcgattag gcgggataac tggctgacct gatgggaaac
     3121 gccgacggca tcctcgacgt cgatgctgaa agcgattccg gtgcccggcg actcgtcgaa
     3181 atccccggca tgctcgatgg tctcgagaat gtcgcgacac aggtgctcct cgaccagcat
     3241 cagtatcacg tcgcgctggg tttcgaggct caggccgagg aatgtcttgg cggtttcgag
     3301 gccctcgccc cgcgcgctcg gaatgacagt ggcaccggta gcgccttttc ccctggcagc
     3361 ctcgacgatg cggtcggtaa tcgagtcctg ggtgagtgcg atgattaact tgaagtgcat
     3421 gactaagcct ccctaggagt agcggttttg tttgcgccgg cctcgccact cggaaagctg
     3481 agcgtaggcg agtaccgaca tgatcggaaa caggctggcg aaagcgatca ggccgaagcc
     3541 gtccagcagc ggattacggc cgggcacggt agcggcgagc cccaggccga gcgcggcgac
     3601 cagcggcacg gttaccgtcg aagtcgtcac gccgccggaa tcgtaagcca gcgctatgat
     3661 catgcgcggc gccagcaggg tttgcagcag cacgaacagg taaccggtaa tgatatacca
     3721 gtacagcggg gtgccggtga tgatccggaa aacgccgagc gcgatgccga ttgccacccc
     3781 gatcgcgacc gcgatgcgca gcccccaggt gtcgatagcg ccggccgaaa cctgggttgc
     3841 cttgatcgct accgcgagca gcgacggctc ggcgaccgtg gtcgaaaaac cgatcgcaaa
     3901 ggcgaacagg taaacccagt ggtagtccca ccaatcggca atctcgccgg agctgccggc
     3961 ggcgatgaaa gccggatcgg tgagttgttg tgccatcagc cggccgagcg ggaacagtgc
     4021 tgactggagt ccttcgagga aaaagccaag cccgatcagt accagcacga agcccttgac
     4081 gattctcgcc aggtggggaa tcggcttctt caggatgaac acctggaaac cgaagaccac
     4141 ggctgctatc ggcagcacgt cctgcaccgt gtagacgatg gttcgcagca ggtcctggaa
     4201 tatctcgagc atcgtatcta taccagcatg ccgtaaccca tgacgaagat catcggtgtc
     4261 agcgaggcaa acgcgatcag accgaagccg tccaccatcg gattgcgtcc cttgatcgag
     4321 cttgccaggc cgacgccaag cgcggtcacc agcggcacgg taatcgtcga cgtggtcacg
     4381 ccaccggaat cgtaggcgat gccgatgatt tcgtgcggtg cgaaggcggt catcgccacc
     4441 acgccgatat agccgacgat gatcaggtat tgaatcggcc agcccatgac tatgcgaaat
     4501 acgccgacga cgatggcgaa gcccaccgcc agggcgacgg tcagccgcag tccgaaagca
     4561 taggaggcca gcgaggactc gctatgctcg atcatgccgc cctgcgccgc gaccagcgcc
     4621 gcctcgccgg cgaccgcgat cagcgccggt tcggccaccg tggtgccgaa gccgagcgca
     4681 aaggcgaaac acagcagcca gaaaagattg ccgcggctgg cgaacgaaaa cgccatggtt
     4741 tcgccgatcg ggaaaagacc gagtttcaat ccctgcacga acagcgtcag gccgataacg
     4801 acgaaagcga tgccgaccac gatctcgccg aaatcgggga tcggctgccg taacacggcg
     4861 agctgaaaga aggcaatgac gacgatgatc ggaagcagat cgcgcaggct gtcgagcagg
     4921 gccgcggcga tgcccttgag tgatttcatt cgcgcccctg gggatctttg atacccacgg
     4981 atattagcaa gctgccggtg cgccctgctg accggtgtca aaaaaaaccc gccctgaggg
     5041 cgggttacgg aatgcttggg gtcgggcttt attcttccat ctttctggtt gccgcttcgc
     5101 cgatgtcgtc gacgccccgc tcctcgagca gcgtggtcag ccagtcggga tccatttccg
     5161 gcaccgatga caacagcagg tcggtgtagg gatgatgcgg tggggtaaac atttcatcct
     5221 tcggcccctg ctcgaccacc tt
//
LOCUS       DATCZV010000549         5308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_680085, whole genome shotgun sequence.
ACCESSION   DATCZV010000549 DATCZV010000000
VERSION     DATCZV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680085"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..492)
                     /gene="moaC"
                     /locus_tag="VIW27_12395"
     CDS             complement(19..492)
                     /gene="moaC"
                     /locus_tag="VIW27_12395"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5740518.1"
                     /translation="MSKLTHFNQAGEAHMVDIGDKQATARRAVAGGRIVMQPATLALI
                     EQGGHKKGDVLGVARVAGIMATKRTAELVPMCHPLALTHVAVDFEIDTDNSAVECTVT
                     AETAGKTGVEMEALTGVQVALLTIYDMCKAVDRGMVIEAVRLLEKAGGKSGHWKR"
     gene            complement(508..1857)
                     /locus_tag="VIW27_12400"
     CDS             complement(508..1857)
                     /locus_tag="VIW27_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018168674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY5740519.1"
                     /translation="MTRLWAILLLTLATPLAAALPAAVDGQQLPTLAPMLERATPAVV
                     NIATRGKSRRRIELPLQNDPFFRRFFDLPDIERIQETSSLGSGVIVDAENGLILTNHH
                     VVENAYEIKVTLNDGRELQAEILGRDPDTDIAVIQVSGEGLSALPLADSSQLRVGDFV
                     VAIGNPFGLGQTVTSGIVSALGRSGLGIESIEDFIQTDASINLGNSGGALVNLRGELV
                     GINTAIYGAGNRGSIGIGFAIPINLARDVMSQLIDYGEVRRGRLGAQGQDLTEQLAQA
                     FGIGRSQGFIITRIESGSPASKAGLRVGDVIVAANDKPIRSAADMHNRVGLQRIGETI
                     ELQLYRDGELIRLPVRIQPIEIRQLDGERLHPRLTGAVLGEMHEQHLQRGSIDYLQLL
                     EVEPGSPAAAAGFLPGDIIYSINKQLTRSFDEALALADEAMIMNVQRGNRELYILLK"
     gene            complement(1867..2787)
                     /locus_tag="VIW27_12405"
     CDS             complement(1867..2787)
                     /locus_tag="VIW27_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY5740520.1"
                     /translation="MKYQDYYQILGVPREADGADIKKAYRKLARKYHPDVNKNADAED
                     RFKQVSEAYEVLKDPARRQAYDRFGADWKHGQQFDETGAGDFHRRAYAGGGFGGGGDF
                     SDFFESIFGSGFQQHGESPFRQSPRRRGADTQLKLDVSLEEAFAGGAKTIQFAAQPGS
                     SEMKKLRINIPKGVGNGQKIRLPRQGQASPNGGEPGDLYLEMNIQPHRLFRLDGRDVI
                     LRLPITPWEAATGATLKVPTLSGSVELKITAGMQSGQKMRLKGKGMPGARPGDQFVEI
                     MIHTPPADDAESKRFYEQMQSRFDFNPRNF"
     gene            complement(2840..4243)
                     /gene="rmuC"
                     /locus_tag="VIW27_12410"
     CDS             complement(2840..4243)
                     /gene="rmuC"
                     /locus_tag="VIW27_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY5740521.1"
                     /translation="MDAIENLHLMLTVAGLVALLLGGLLYGLRQAHNASRESKRDLDE
                     ARRQELELREALHASEKQVQGLQLELSTERRMIKQQVEQYEKNRQQLKIEFENLANRI
                     LEDKTRAFDKTNKTALESLLKPFREQVEGFQKRINEVHGESLQGNTRLESEIRKVLEI
                     GLQMSGEAKNLTNALKGDSQRRGSWGETQLEKTLQLAGLIENAHYEKQTSMYDGEGRR
                     KQTDFLIKLPDDKHIVIDSKVSLAAYDRAVGAETEEQQQRALGEHVRAVKTHIDSLHS
                     KDYTNLIGIRSPSFVLMFMPIEPAYIEALKYEKDLFSYGYDKNIVLVSHTTLIPILRT
                     VANLWAMERSTREARELGDKAGDIYNQVRRVAQRLDKLGGSLNAAVNHYNGTVKSVAG
                     RQGLHGKVERFSELSSKVTESLPELEPLTAYAERERLELLAEPLADTEEAASLPRPGD
                     EDGETLAEPLEDEASVT"
     gene            complement(4352..>5308)
                     /locus_tag="VIW27_12415"
     CDS             complement(4352..>5308)
                     /locus_tag="VIW27_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740522.1"
                     /translation="EVRRLRSQAVAVDVKPIPEAYDAAYWLPARKLELREQWQVDTDN
                     LVAGEPVTRSLTLLADGLTAAQLPELRLEPVDGIKQYPDQPSLENQRTNAGIRGSREQ
                     KVALIPGSAGRYLLPPIELPWWNLETGRQEVALLPAREILVQPAPGAADTAAPPPEVA
                     ASGGETAAPAPPATERSLWPWLSLVLATGWALSLLYWWFRSRRARLIPVAATEVVSLR
                     RARRDLQQACDADDATAARAALLAWGRALLAPRPIANLQQLCAVLGEDLQREVEDLNR
                     SRYARSQHAWQGASLWRLCRRLEAGQARDRGQALELLPLNPR"
BASE COUNT          853 a         1728 c         1681 g         1046 t
ORIGIN      
        1 aggcgcgctg acctcgcttt atcttttcca gtgtcccgac ttgccaccgg ctttttccaa
       61 cagccgcacg gcctcgatga ccataccgcg gtcgaccgcc ttgcacatgt cgtaaatcgt
      121 cagcaacgcg acctgtacgc cggtgagcgc ttccatctcg acccctgtct tgccggcggt
      181 ctcggcggtg accgtgcact ccaccgcgct gttgtcggta tcgatctcga aatcgacggc
      241 cacgtgggtc agcgccagcg ggtgacacat cggcaccagc tcggcggtgc gcttggtcgc
      301 catgatgccg gcgacacgcg cgacaccgag cacatcgccc ttcttgtggc cgccctgctc
      361 gatcagcgcc agggttgccg gctgcatgac gatgcgacca ccggccaccg cgcggcgcgc
      421 ggtcgcctgc ttgtcgccga tatcgaccat gtgcgcctcg ccggcctggt tgaagtgggt
      481 gagtttgctc atttgccggc tagtctatca ttttaaaagg atatatagct cgcgattgcc
      541 gcgctggacg ttcatgatca tcgcctcgtc ggccagtgcc agggcctcgt cgaaactgcg
      601 cgtcagctgc ttgttgatcg agtagatgat gtcacccggc aaaaagccgg cggcggcggc
      661 cggcgaaccc ggctcgacct cgaggagttg caggtaatcg atgctgccgc gctgcaggtg
      721 ctgctcgtgc atttcaccga gcaccgcgcc ggtgagccgc ggatgcagcc gttcgccgtc
      781 gagctgtcgg atttctatcg gttgaatgcg caccggcagg cggatcagct cgccgtcgcg
      841 gtagagctgc aattcgatcg tctcgccgat acgctgcagc ccgacgcggt tgtgcatgtc
      901 ggccgccgag cgaatcggct tgtcgttggc ggcgacgatc acgtcgccga cacgcagacc
      961 ggccttcgac gccggcgatc cggactcgat tcgagtgatg ataaagccct ggctgcggcc
     1021 gataccgaaa gcctgcgcga gttgctcggt caaatcctgc ccctgggcac cgagtcggcc
     1081 gcggcgtacc tcgccgtaat cgatcaactg cgacatcacg tcgcgcgcca ggttgatcgg
     1141 aattgcgaaa ccgataccga tactgccgcg gttgccggcg ccatagatcg cggtgttgat
     1201 gcccaccagt tcaccgcgca ggttgaccag cgctccaccc gaattgccga ggttgatcga
     1261 cgcatcggtc tggatgaaat cctcgatcga ctcgatgccg agtccggaac ggccgagcgc
     1321 ggagacgatt cccgaggtca cggtctgccc gagaccgaac gggttgccga tggcgaccac
     1381 gaagtcgccg acccgcagct ggctcgagtc agcaagcggc agcgccgaca ggccttcacc
     1441 ggagacctgg atcacggcga tgtcggtatc cggatcgcgg ccgaggattt cggcctgcag
     1501 ctcgcggccg tcgttgaggg tgaccttgat ttcgtaggca ttttcgacaa cgtggtggtt
     1561 ggtcaggatc agtccgtttt cggcatcgac gatgaccccg gagccgagac tggaggtttc
     1621 ctggatgcgt tcgatgtccg gcagatcgaa aaaacgccgg aaaaacgggt cgttctgcag
     1681 cggcagctcg atccgccggc gggacttgcc acgcgtcgca atattgacca ccgccggcgt
     1741 ggcgcgctcg agcatcggcg ccagggtcgg caattgctgg ccgtcgaccg ccgccggcaa
     1801 ggccgccgcc agcggggtgg cgagggtcaa cagcaaaata gcccacagac gcgtcatcga
     1861 gtggcgtcag aagttgcgcg gattgaagtc gaaacgggac tgcatctgtt cgtaaaagcg
     1921 cttcgattcg gcgtcgtcgg ccggcggcgt gtggatcatg atttcgacga actgatcgcc
     1981 gggcctggca ccgggcatgc ccttgccctt gaggcgcatc ttctgaccgg actgcatacc
     2041 ggcggtgatc ttcagttcca ccgatcccga cagggtcggc accttcagcg tggcgccggt
     2101 agcggcctcc cagggtgtaa tcggcaggcg caggatcacg tcgcgaccgt cgagccggaa
     2161 cagccggtgc ggttggatgt tcatttccag gtaaaggtcg cccggctcgc cgccgttcgg
     2221 cgaagcctgg ccctgccggg gcaggcgtat cttctgcccg ttaccgacac ccttcggtat
     2281 attgatcctg agcttcttca tctccgagga acccggctgg gcagcgaact ggatcgtctt
     2341 ggcgccgccg gcgaaggctt cctcgaggct gacatcgagc ttcagctggg tatccgcacc
     2401 acggcggcgc gggctctggc gaaacggcga ttcgccgtgc tgctgaaagc cgctgccgaa
     2461 tatcgattcg aaaaaatcgc tgaaatcgcc gccaccaccg aagccgccgc ccgcgtaggc
     2521 acggcgatgg aaatcaccgg ccccggtctc gtcgaactgc tggccgtgct tccagtcggc
     2581 gccgaaacgg tcgtaagcct gccgcctggc aggatccttg agcacctcgt aggcctcgct
     2641 gacctgcttg aaacgatctt cggcatcggc attcttgttg acatccggat ggtacttacg
     2701 cgcaagcttg cggtaagcct tcttgatgtc ggcgccatcg gcctcgcggg gaacgcctaa
     2761 gatttggtaa taatcttgat atttcacgta cttaacgcac cgggacgatc cctgataaat
     2821 aggatttttt agcggatttt caagttaccg aagcttcgtc ctcgagcggc tcggccagcg
     2881 tttccccgtc ctcgtcgcca ggccgcggca ggctcgcggc ctcttccgtg tcggccagcg
     2941 gttccgccag caactcgagg cgctcgcgtt cggcgtatgc cgtcaggggc tccagttcgg
     3001 gcagggattc ggtcacctta ctcgatagtt ccgaaaagcg ctccactttt ccgtgcagcc
     3061 cctggcgccc cgccaccgac ttgaccgtcc cgttatagtg gttgaccgcc gcattcaaac
     3121 tgccgccgag cttgtccagt ctctgcgcaa cgcgccttac ctggttgtag atatcgcccg
     3181 ccttgtcgcc gagttcgcgc gcctcgcgcg tgctgcgctc catcgcccac agattggcga
     3241 cggtgcgcag aatcgggatc agggtcgtat gcgacaccag cacgatgttc ttgtcgtagc
     3301 cgtaagagaa caggtccttc tcgtatttca gcgcctcgat gtaggccggc tcgatcggca
     3361 tgaacatgag cacgaagctc gggctgcgaa taccgatcag gttggtgtaa tccttggagt
     3421 gcaggctgtc gatatgcgtc ttgaccgcgc gcacatgctc gccgagcgcc cgctgctgct
     3481 gttcctcggt ttcggcgccg accgcgcggt cgtaggcggc gagcgaaacc ttgctgtcga
     3541 tgacgatatg cttgtcgtcc ggcagcttga tcaggaaatc ggtctgcttg cgccgcccct
     3601 cgccatcgta catcgaggtt tgcttctcgt agtgtgcgtt ctcgatcagt ccggccagtt
     3661 gcagggtttt ctcgagctgg gtctcgcccc agctgccgcg ccgctgcgag tcgcccttca
     3721 gcgcattggt caggttcttg gcctcgccac tcatctgcag gccgatctcg agcaccttgc
     3781 ggatctcgct ctcgaggcgg gtattgccct ggagcgactc gccgtggacc tcgttgatgc
     3841 gcttctggaa accctccacc tgctcgcgga agggtttcaa cagcgactcc agcgcggtct
     3901 tgttggtctt gtcgaaggca cgcgtcttgt cctcgagaat gcgattggcg agattctcga
     3961 actcgatctt cagctgctgc cggtttttct cgtactgctc gacctgctgc ttgatcatcc
     4021 tgcgctcggt cgacagttcg agttgcaagc cctgcacctg cttttccgat gcgtgcagcg
     4081 cttcgcgcaa ttcgagctcc tggcggcgcg cttcgtcgag atcgcgtttc gactcgcggc
     4141 tggcgttatg ggcctggcgc agcccgtaga gcaagccgcc gagcagcagc gcgacaagac
     4201 cggcgacggt tagcatcagg tggagatttt cgatggcatc cataagggtt tttcgacgct
     4261 ttgatcgcct cagtctaaac cagttagtgg ctcaccgcga gagcccgatt atacccggac
     4321 agcccgttcg cgggcgcccc tggctgcgcg gtcagcgggg attcaacggc aacagttcga
     4381 gcgcctggcc gcggtcgcgc gcctgcccgg cttccagccg ccggcacaac cgccacaggc
     4441 tcgctccctg ccaggcgtgc tgtgagcgcg cgtagcggct gcggttgagg tcctcgacct
     4501 cgcgctggag atcttcgccg agcaccgcgc acagctgctg caggttggcg atcgggcgcg
     4561 gcgccagcag cgctcgcccc cacgcgagca gtgccgcgcg ggccgcagtg gcgtcatcgg
     4621 cgtcgcaggc ttgctgcaga tcacggcgcg cgcggcgcag cgataccact tcggtggcgg
     4681 caaccgggat cagccgcgcg cggcgcgacc tgaaccacca gtaaagcagg ctcagggccc
     4741 agccggtggc cagcaccagg ctcaaccacg gccacagcga acgctcggtg gcgggcggcg
     4801 ccggcgctgc cgtttcgccg ccgctcgcgg cgacctcggg tggcggcgcg gcggtgtcag
     4861 cggcgccggg cgctggctgc acgaggattt cgcgcgccgg cagcaacgcg acctcctggc
     4921 ggccggtctc gagattccac cacggcaact cgatcggcgg cagcaggtag cgtcccgcac
     4981 tgcccggaat cagcgcaacc ttttgctcgc ggctgccgcg aatgccggcg ttggtgcgct
     5041 ggttttcgag cgacggctga tccgggtatt gcttgatgcc atcgaccggc tcgagccgca
     5101 attccggtaa ctgtgccgcg gtcaggccat cggcgagcag cgtcaggctg cgggtgaccg
     5161 gttcgccggc aaccaggttg tcggtgtcga cctgccactg ctcgcgcaat tccagcttgc
     5221 gtgccggcaa ccagtaggcc gcgtcgtagg cctccggtat cggcttgaca tcgaccgcga
     5281 ccgcctgcga gcgcagccgc cgcacctc
//
LOCUS       DATCZV010000550         8715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_786535, whole genome shotgun sequence.
ACCESSION   DATCZV010000550 DATCZV010000000
VERSION     DATCZV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786535"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..2129)
                     /gene="acs"
                     /locus_tag="VIW27_12420"
     CDS             complement(183..2129)
                     /gene="acs"
                     /locus_tag="VIW27_12420"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5740523.1"
                     /translation="MSEIKTYPVPPEFAAQANVNAEQYQAMYRQSIEDPEGFWSEQAE
                     NYISWFKPWNTVSDWSFEADDLHIKWFDGAELNVSYNCLDRHLETRGDQVAIIWEGDD
                     PGEDRKITYAELHAEVCKFGNALKSRGVKRGDRVSIYMPMVPEAAVAMLACTRIGAMH
                     SVVFGGFSPDALRDRILDSDCQVVITADQSIRGGKKVPLKANADRAVSQCPNVSTVFV
                     VQRGGDPVDWTEGRDVWYHEAIAEASADCEPEHMAAEDPLFILYTSGSTGKPKGVMHT
                     TGGYLLQAAMTHKLVFDYKDGEIYWCTADVGWVTGHTYIVYGPLANGATTLMFEGIPT
                     YPDISRFWQVCDKHDVSIFYTAPTAIRSLMGAGDEPVNQTSRSSLRLLGTVGEPINPE
                     AWLWYYNVVGNGKCPIVDTWWQTETGAHMMTPLPGATDTKPGSATFPFFGVQPVLLDD
                     EGNELDGNPASGNLAIRTPWPSMMRTVYGDHKRFYETYFAMFKGYYFTGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVLHEKVAEAAVVGYPHELTGQGIYAYVTLM
                     AGEEGTDELKAELVKLVRSEIGPIAKVNIIQWAPGLPKTRSGKIMRRILRKVAANELD
                     NLGDTSTLADPGVVDNLIDNRENR"
     gene            2336..2536
                     /locus_tag="VIW27_12425"
     CDS             2336..2536
                     /locus_tag="VIW27_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740524.1"
                     /translation="MSQFSSDLRPSEITIVVVLFAALVLLPPLLGLWFSEDKPWYLPY
                     LVWLGIIGLSYSLQRLLRRNAI"
     gene            2526..5465
                     /locus_tag="VIW27_12430"
     CDS             2526..5465
                     /locus_tag="VIW27_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5740525.1"
                     /translation="MQFELWQLFVVGVLYLGLLFVIATATDRGWIPKSVARHPLVYTL
                     SIGVYATSWSFYGSVGFAEKEGFNFLTIYLGTTLAFVASPILLRPILKLTETYRLASL
                     ADLFAFRYHSQLAGVLVTLVVLTGTLPYISLQIQAVTNTLQIMSNETEQQLIALMFCI
                     TVGLFSILFGARHISLREKHRGLVVAIAFESLVKLVTLLTVGLFAVFGIFGGFGGLNQ
                     YLHEHPEANQALFAPVSEGPWATLMLLSFAAAFLLPRQFHMLFVESIEHKNIETASWA
                     FPLFLLLLNLSIPPILWAGQSLQLSVPADFYVLGITLESDTSVLPILTFMGGLSAASA
                     MIIVTTLALASMCMNNLVLPASFPPKLSRGTNMYEWLLWGRRLVIMSIVGIGYTFYLV
                     QQHNPGLAGLGLVSFVAVAQCLPGVIGLLYWPRANKTGFIWGLLVGATIWFFSLFLPL
                     FERAGIIETPFFTTPELAMSAPDAALWSLVLNVLTFVIVSLLTTQSLDERQVADACCR
                     ESFLIPRGEIKAVSIAEFEKQLGSIVGNDIAHNEVDRALADLGLAPGEITREDLLNLR
                     AQIDRNLSGLVGPVLSRMIVNEHLQIDRATQSALADTIQFVGDSRERSRSELRGVSSE
                     LDALRRFHFQVLQDLPLGAVSIDNDNIIVSWNKQLSRITGIDQQHAVAKSLDQLDSPW
                     RELLAGFIASEQFLISKFKLPLHGRTRVLNLFKAVVSQETGAGGSGIAILVEDHSERY
                     SLEEKLAHNERLASIGQLASGVAHEIGNPVTGIACLAQDLRAELADAPQAGEGLEQIL
                     VQTERISRIVRSLSNFSHAGTPDEYPVESLEVAQIVGEAVNLVSLSQAGKQMQYENGC
                     ERDLMISGYAHKLVQVFVNLLTNATDASEPGQKVRVAAERRGDYVSISVSDHGKGIPE
                     NHLPRVFEPFFTTKTVGEGTGLGLSLTYNIVQEHGGSITVSSIAGRGCRFEILLPGAD
                     ESLAVQV"
     gene            5462..6799
                     /locus_tag="VIW27_12435"
     CDS             5462..6799
                     /locus_tag="VIW27_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5740526.1"
                     /translation="MSNILVVEDEPIIQESLVRLLKRQGHDVTGVASVAEAEENSINT
                     YDLIISDLRLPGEPGTELIARAAPTPVLIMTSYSTVQSAVEAMKQGAVDYISKPFNHD
                     EMIMTVKRILDKTRVEQQNTLLREEVARYYPIDGMVAGCDVMHEVLDRARRVAPADTT
                     VLIVGETGTGKELVARAIHAQSGRRKQSLVNVNCAAFAESQIESELFGHERGAFSGAL
                     QMKKGLIETANGGTLFLDEIGELPLESQGRLLRVLQHNEMRRVGANKLIKVNVRILAA
                     THKNLAHMVEQGSFREDLFFRLNVFDINLPPLRERGIDIRLLADRILQRMAQRAHRNP
                     MRFTDGAYRQLEQYVWPGNVRELENVLERAVILQQGDCIEAEDLSLDNVETGHRLPQY
                     ELENRLSLDDYFVSFVMKYQHSYNETELARMLGISRKNLWEKRQRLDIPARKS"
     gene            6810..8552
                     /gene="aceK"
                     /locus_tag="VIW27_12440"
     CDS             6810..8552
                     /gene="aceK"
                     /locus_tag="VIW27_12440"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEY5740527.1"
                     /translation="MQLSDKSLAMEIATTILNGFDRHFSIFSEITRGAKQRFESADWQ
                     AEREAARERIEFYDHRVRDAIAELQKLFELEPFNAQLWFDVKREYVQLISNHLQPELA
                     ETFYNSVFCALFHRDYFDNDFIFVRSAVSTEFIDSEKPSYHVYYPAKRGFRASIKQVL
                     LDSGFDLPFEDIDRDVRNILKSLIRHDFKRPRRAHLNFQFQVISALFYRNKAAYIVGR
                     AINSHQQVPFAIPILHSEGQSIYCDALIIGIQGLSRLFEFSYAYFMVDHPVPSSIVTF
                     LRRLIPTRNKESFYSAIGFQKQGKTVFYRDFLHHLKHSEDQLIVAPGIRGMVMAVFTL
                     PSYPYVFKVIRERFPPPKRTTRQEVLETYRLVKRHDRVGRMSDILEYSHVALPRNRFD
                     DALLEELYETCANSVSDEGDYIVIKHLYIERRMTPLNLAVQQADDAYLDHLMKGYGLA
                     IKELAAANIFPGDLLMKNFGVTGQGKGRIVFYDYDEICYLTECNFRDIPEARYPEDEL
                     AAEPWYSIAANDIFPEQFATFLFADRRVRETFLKHHADLLRADFWIRKQENIKAGIYE
                     DVFPYPQKIRFRRD"
     gene            complement(8549..8710)
                     /locus_tag="VIW27_12445"
     CDS             complement(8549..8710)
                     /locus_tag="VIW27_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740528.1"
                     /translation="MGVRALATFPRRGTRDGLGDVDVEVEFLGAVFRPREFLYCDEDG
                     ILLSPVELI"
BASE COUNT         1684 a         2723 c         2575 g         1733 t
ORIGIN      
        1 aggataccgc cggaccgcgt ggatttgaca acagtgaaga ggatcggtcg aacaaccgca
       61 gccgagcggc ttcgaagtgc tcccttaatc cataacgccg gcgcggatat gcggaaaacc
      121 cagagcggct agaaaaacag gccccgcgat cgcggggccc gtgttttacg gcaggaacga
      181 gcttagcggt tttcgcggtt atcgatcagg ttgtcgacca ccccggggtc ggccagtgtc
      241 gaagtatcgc cgaggttgtc gagttcgttg gcggccacct tgcgcagaat gcggcgcatg
      301 atcttgccgg aacgggtctt tggcagcccc ggggcccact ggatgatatt gaccttggcg
      361 atcggcccga tctccgagcg caccagcttg accagttcgg ccttcagctc gtccgtgccc
      421 tcctcgccgg ccatcagtgt cacgtaggcg tagatgccct gcccggtcag ttcgtgcggg
      481 taaccgacca ccgcggcctc ggcaaccttt tcgtgcagca ccagcgcgga ttcgatttcc
      541 gcggtgccga ggcggtgacc ggagacattg agcacgtcgt cgacacgccc ggtgatccag
      601 taatagccat cttcatcgcg gcgcgcgccg tcgccggtaa aataatagcc cttgaacatc
      661 gcgaaatagg tttcatagaa gcgcttgtgg tcaccgtaaa cggtgcgcat catcgatggc
      721 cacggcgtcc tgattgcgag gttgccgctg gcgggattgc cgtcgagttc gttgccctcg
      781 tcgtcgagca gcaccggctg cacaccgaag aacggaaagg tcgcggaacc cggtttggta
      841 tcggtcgcgc ccggcagcgg cgtcatcatg tgcgcgccgg tctcggtctg ccaccaggta
      901 tcgacgatcg ggcacttgcc gttgccgacg acgttgtaat accacagcca ggcttccggg
      961 ttgatcggct cgccgaccgt accgagcagg cgcaggctgc tgcgcgaagt ctggttgacc
     1021 ggctcgtcgc cggcgcccat cagcgaacgg atcgcggtcg gcgcggtata gaaaatcgac
     1081 acgtcgtgct tgtcgcaaac ctgccagaag cgtgaaatgt ccgggtaagt cggaatgccc
     1141 tcgaacatca gcgtggtggc gccgttggcg agcgggccat agacgatgta ggtatgaccc
     1201 gtcacccagc cgacgtcggc cgtacaccag tagatttcgc cgtccttgta gtcgaacacc
     1261 agcttgtggg tcatcgccgc ctgcagcagg taaccgccgg tggtatgcat gacgcccttg
     1321 ggcttgccgg tcgagcccga ggtataaaga atgaacagcg ggtcttccgc ggccatgtgc
     1381 tcgggctcgc aatcggcaga cgcctcggcg atggcctcgt ggtaccagac gtcgcggcct
     1441 tcggtccagt cgacgggatc gccgccgcgt tgcacgacga acacggtgct gacgttcggg
     1501 cactggctga cggccctgtc ggcatttgcc ttcagcggca cctttttgcc gccgcggatc
     1561 gactgatcgg cggtgatcac cacctggcag tcggaatcga gaatgcggtc gcgcagcgcg
     1621 tcgggcgaaa agccgccgaa aaccaccgag tgcatcgcgc cgatacgcgt gcaggcgagc
     1681 atcgccaccg ccgcttcggg gaccatcggc atgtagatcg atacccggtc gccgcgcttg
     1741 acgccgcggc tcttcagcgc attgccgaat ttgcagactt cggcgtgcag ctcggcatag
     1801 gtgatcttgc ggtcctcgcc gggatcgtcg ccttcccaga tgatcgcgac ctggtcgcca
     1861 cgcgtttcga gatgccggtc gaggcaattg taggatacgt tgagttcggc gccatcgaac
     1921 cacttgatgt gcaggtcgtc ggcttcgaaa ctccagtcgc tgaccgtgtt ccacggcttg
     1981 aaccaggaga tatagttttc cgcctgctcg gaccagaaac cttccgggtc ctcgatcgac
     2041 tggcgataca tcgcctggta ttgctcggcg ttgacgttgg cctgcgccgc gaactccggt
     2101 ggcacgggat aggttttgat ttctgacatc gctccttcct cgggaattgg ttattggtct
     2161 gggttgagtg ccgaatatgc ttacataatc gccgcccagc gacaagttgc tgacgcgccc
     2221 ccgtcacgct gttttgcgcc ggcttgccgc atgtcaattg gcgcgcgagc gatgttgacc
     2281 tgactgtcac gataggtaac actttcggtc cccggaagta accctgggta acaccatgtc
     2341 gcagttttca tccgatctgc gtccttccga gataaccatc gtcgtcgtac tgttcgcggc
     2401 gctggtgctg ctgccgccgc tactcggcct gtggttctcg gaggacaaac cctggtacct
     2461 gccctacctg gtctggttgg gcatcattgg gctatcgtac tcgctgcagc gcctgctgcg
     2521 cagaaatgca atttgagctc tggcagctgt tcgttgtcgg ggtgctgtat ctcggcctgc
     2581 tgttcgtcat cgccacggca accgaccgtg gctggatacc gaaatcggtg gcccgccacc
     2641 cgctggtcta caccctgtcg atcggcgtct acgcgacttc gtggagtttc tacggcagtg
     2701 tcggcttcgc cgaaaaggaa ggcttcaatt tcctgaccat atacctggga acgacgctgg
     2761 cgttcgtcgc ttcgccgata ctgctgcggc cgatactgaa actcaccgaa acttaccggt
     2821 tggcgtcgct cgccgacctg ttcgcgtttc gctaccacag ccagctcgcg ggagtactgg
     2881 tgacgctggt ggtgctgacc ggcaccctgc cgtacatttc gttgcaaatt caggcggtta
     2941 ccaacaccct gcagatcatg agcaacgaaa ccgagcagca actgatcgca ttgatgttct
     3001 gcattaccgt cggcctgttt tcgatcctgt tcggtgcccg ccatatcagc ctgcgcgaaa
     3061 aacaccgcgg cctggtggtc gcgatcgcct ttgagtcgct ggtcaagctg gtgaccctgc
     3121 tgaccgtcgg cctgttcgcg gtattcggca tcttcggcgg cttcggcgga ctgaaccagt
     3181 acctgcacga acatccggaa gccaaccagg cgctgttcgc gccggttagc gaaggcccct
     3241 gggcaacgct gatgctgctg tcattcgccg ccgcgttcct gctgccgcgc cagtttcaca
     3301 tgctgttcgt cgagagcatc gagcataaga atatcgagac cgcgagctgg gcgttcccgc
     3361 tgttcctgtt attactgaac ctgtcgatac ccccgatcct gtgggcaggc cagtctctgc
     3421 agttgtcggt tccggccgac ttctacgtgc tcggcatcac cctcgagagc gacacgtcgg
     3481 tgctgccgat actcaccttc atgggcggcc tgtcggcggc cagcgcgatg attatcgtca
     3541 ccacgctggc gctggcgtcg atgtgcatga acaacctggt gctgccggcg agcttcccgc
     3601 ccaagttgtc acgcggcacc aacatgtacg aatggctgct gtgggggcgg cgcctggtga
     3661 tcatgtcgat cgtcggtatc ggctatacct tttacctggt gcagcagcac aatcccggac
     3721 ttgccggtct cgggctggtg tccttcgtcg ccgtcgcgca atgcctgccc ggggtgatcg
     3781 ggctgctgta ctggccgcgc gccaacaaga ccggcttcat ctgggggctg ctggtcggcg
     3841 cgaccatctg gtttttcagc ctcttcctgc cgttgttcga gcgcgccggc atcatcgaga
     3901 ccccgttctt caccactccg gaactggcga tgagcgcgcc cgacgccgcg ctctggtcgc
     3961 tggtgctcaa cgtgctgact ttcgtcatcg tctcgctgct gaccacgcag agcctcgacg
     4021 agcgccaggt ggccgacgcc tgctgccggg agagcttttt gatcccgcgc ggcgagatca
     4081 aggcggtatc gatcgccgaa ttcgaaaaac agctgggcag tatcgtcggc aacgatatcg
     4141 cgcataacga ggtcgaccgg gcgctcgccg acctcgggct cgcgcccggg gaaatcaccc
     4201 gtgaagacct gttgaacctg cgcgcacaaa tcgatcgcaa cctgtccggc ctggtcggac
     4261 cggtgctgtc gcgcatgatc gtcaacgagc acctgcagat cgaccgcgcc acgcaatccg
     4321 cacttgccga taccattcaa ttcgtcggcg acagccgcga gcgttcgcgc agcgaattgc
     4381 gcggggtctc gagcgaactc gacgccctca ggcgttttca tttccaggta ctgcaggatt
     4441 tgccgctggg cgcggtatcg atcgacaacg acaatatcat cgtcagctgg aacaagcagc
     4501 tgagccgcat caccggcatc gatcagcagc atgcggtcgc caagtcgctc gaccagctgg
     4561 attcgccttg gcgcgaactg ctggcaggat tcatcgccag cgagcagttc ctgatcagca
     4621 agttcaaact gccgctgcac ggccggacgc gagtgctgaa cctgttcaag gccgtcgtca
     4681 gccaggaaac cggcgccggc ggcagcggta tcgcgatcct ggtcgaggat cacagcgagc
     4741 gttactcgct ggaggaaaag ctcgcgcaca acgaacgcct ggcgtcgata ggacaactgg
     4801 cctcgggcgt cgcccacgaa atcggcaacc cggtaaccgg catcgcctgc ctggcgcagg
     4861 acctgcgcgc ggaactcgcc gatgcgccgc aggccggtga aggcctggaa cagattctcg
     4921 tacagaccga gcgcatttcg cggatcgtca ggtcgctgtc gaatttcagc catgccggca
     4981 cccccgacga atatccggtt gagagcctcg aggtggcgca aatcgtcggc gaagccgtca
     5041 acctggtgtc gttgagccag gccggcaagc agatgcagta cgaaaatggc tgcgagcgcg
     5101 atctgatgat ctcgggttat gcgcacaaac tggtgcaggt attcgtcaac ctgctgacca
     5161 acgctacgga cgccagcgag cccgggcaaa aggtcagggt cgcggcggag cgccgcggcg
     5221 actatgtctc gatcagtgtc agcgaccacg gcaaggggat acccgagaat cacctgccgc
     5281 gcgtattcga gccatttttc accaccaaga cggtcggtga aggcaccggt ctcggcctgt
     5341 cgctgacata caatatcgtc caggaacacg gtggatcgat taccgtcagc agtattgcgg
     5401 gacgcggttg ccgtttcgag atactgctgc ccggggccga cgaatcgttg gcagtgcagg
     5461 tatgagcaat attctggtcg tcgaggatga accgatcatt caggaaagcc tggtgcgcct
     5521 gctcaagcgc caggggcacg acgtcaccgg cgtcgcctcg gttgccgagg ccgaggaaaa
     5581 ctcgatcaac acctacgacc tgatcatctc cgacctgcgc ctgcccgggg agcccggcac
     5641 cgagctgatc gcgcgcgcgg cgccgacgcc ggtactgatc atgaccagtt actcgaccgt
     5701 gcagtcggcg gtcgaggcga tgaaacaggg cgcggtcgac tatatctcca agcccttcaa
     5761 tcacgacgaa atgatcatga ctgtcaagcg catcctcgac aagacccgtg tcgaacagca
     5821 aaacacgctg ttgcgcgagg aagtcgcccg ctactacccg atcgatggca tggtcgccgg
     5881 ttgcgacgtg atgcacgagg tgctggaccg tgcacgccgt gtcgcgcctg ccgataccac
     5941 cgtgctgatc gtcggcgaga ccggtaccgg caaagaactg gtggcgcgcg cgattcacgc
     6001 ccagagcggt cggcgcaagc aatcgctggt caacgtcaat tgcgcggcgt ttgccgaaag
     6061 ccagatcgaa tcggaattgt tcggccacga acgcggcgcc ttcagcggcg cgttgcagat
     6121 gaagaagggc ctgatcgaaa ccgccaacgg cggtacgctg ttcctcgacg agatcggtga
     6181 attacccctc gaatctcagg ggcgcctgct acgggtgctg caacacaacg agatgcggcg
     6241 cgtgggtgcg aacaagctca tcaaggtcaa cgtgcgcata ctggctgcga cccacaagaa
     6301 cctcgcgcac atggtcgagc agggatcgtt ccgcgaagac ctgttctttc gcctcaacgt
     6361 gttcgatatc aacctgccgc cgctgcgcga gcgcggtatc gacatcaggc tgctggccga
     6421 ccgcattctg cagcgcatgg cacagcgtgc ccaccgcaat ccgatgcgct tcaccgacgg
     6481 cgcctaccgc caactcgagc aatacgtctg gccaggtaac gtgcgcgagc tcgaaaatgt
     6541 cctcgagcgc gcggtgatcc tgcagcaggg cgattgcatc gaagccgagg acctgtcgct
     6601 ggacaacgtc gaaaccggcc accggctgcc ccagtacgag ctcgagaacc gcctgtcgct
     6661 ggacgattac ttcgtcagtt tcgtcatgaa gtaccagcat agttacaacg aaaccgagct
     6721 cgcgcgcatg ctcggcatca gccgcaaaaa cctgtgggaa aagcgccagc gcctcgacat
     6781 tccggcgcgt aaatcctgag agcccagtga tgcaactctc cgacaaaagt ctggcgatgg
     6841 aaatcgccac cactatcctc aacgggttcg atcgccactt cagcattttc agtgaaatca
     6901 cccgcggcgc gaagcagcgt ttcgaaagcg ccgactggca ggccgaacgc gaggccgcgc
     6961 gcgaacgcat cgagttttac gatcaccgcg ttcgggacgc aatcgccgaa ctgcaaaagc
     7021 tgttcgaact ggagccgttc aatgcccagc tatggttcga cgtcaagcgc gaatacgtgc
     7081 agctgatcag caatcacctg caacccgaac tcgccgagac cttttacaac tcggtgttct
     7141 gcgcgttatt ccaccgcgac tatttcgaca acgatttcat cttcgtacgc agcgcggtat
     7201 cgaccgaatt catcgattcc gagaaaccgt cgtaccatgt ctactatcct gccaagcgcg
     7261 gtttccgcgc atccatcaag caggtcttgc tcgacagcgg cttcgatctg ccgttcgagg
     7321 atatcgatcg cgatgtgcgc aatatcctga aatcgctgat caggcatgat ttcaagcgcc
     7381 cgcggcgcgc gcacctgaat ttccagtttc aggttatcag tgcgctgttt taccgcaaca
     7441 aggccgctta catcgtcggt cgggcgatca attctcatca gcaggtgccg ttcgcgatcc
     7501 cgatcctgca cagcgaagga caatcgattt attgcgatgc gctgatcatc ggcatccagg
     7561 gattaagccg cctgttcgaa ttctcctatg cctacttcat ggtcgatcac ccggtaccgt
     7621 cgtcgatcgt gacctttctg cgacgcctga taccgacccg caacaaggaa tccttttatt
     7681 ccgcgatcgg ctttcagaag cagggcaaga cggtatttta tcgcgacttc ctgcaccacc
     7741 tgaaacattc cgaggatcag ttgatcgtcg cgcccggcat tcggggaatg gtgatggcag
     7801 tgtttaccct gccctcttat ccgtacgtct tcaaggtcat ccgcgaacgc tttccgccac
     7861 cgaagcgaac cacgcgccag gaagtgctcg aaacctaccg cctggtcaag cgtcacgacc
     7921 gggtcggccg catgtccgac attctcgagt actcgcacgt tgcgctgccg cgcaaccgtt
     7981 tcgacgacgc tctgctcgag gaactctacg aaacctgtgc caacagcgtt tccgacgagg
     8041 gcgattacat cgtcatcaag cacctatata tcgaacggcg catgacgccg ctcaacctcg
     8101 ccgtgcaaca ggctgacgac gcgtacctcg atcacctgat gaagggctac ggcctggcga
     8161 tcaaggagct ggccgcggcc aatatctttc cgggcgatct gctgatgaag aatttcggcg
     8221 tcaccggcca gggcaagggg cgcatcgtgt tctacgatta cgacgaaatc tgctacctga
     8281 cggaatgcaa ttttcgcgac attcccgagg cgcgttaccc cgaggacgaa ctcgccgccg
     8341 agccctggta cagcatcgcc gccaatgaca tctttccgga gcagttcgcg acctttctgt
     8401 ttgccgaccg gcgtgtgcgc gagaccttcc tcaaacacca tgccgatctg ctgcgtgccg
     8461 atttctggat tcgcaagcag gaaaatatca aggccggcat ctacgaggac gttttcccct
     8521 acccgcagaa gatccgcttt cggcgcgact agatcagctc cacgggtgac agcaagatgc
     8581 cgtcctcatc gcaatagagg aattctcgag gacggaacac cgcgccgagg aattcgacct
     8641 cgacatcgac gtcaccgagg ccgtcccggg tgccgcgccg tgggaaagtc gccagcgccc
     8701 ggacgccgat atcca
//
LOCUS       DATCZV010000551         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_309100, whole genome shotgun sequence.
ACCESSION   DATCZV010000551 DATCZV010000000
VERSION     DATCZV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309100"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VIW27_12450"
     CDS             complement(<1..873)
                     /locus_tag="VIW27_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5740529.1"
                     /translation="MTTKIWAASALLATGWADSVEISIAEDGSIEAVAPDSDYISGAR
                     AEVLIPAVPNVHSHAHQRAMAGLGERAGNNEDSFWTWRKVMYHYLERIQPDHLHHIAA
                     QLYLEMLKAGYSCVGEFQYLHHDVAGKAYTNPAEMSLQCLQAARSVGLGFTALPVLYR
                     YGGFGSVAPLDGQKRFINKTDDFVGIVDSLQQATVGDANASVGIAPHSLRAIDRELLN
                     EVIGSLDRLAAIHIHVAEQTKEVDDCLAWSGQRPVAWLFDNFDVNSDWCLIHATHMDA
                     AETRSMAASGCIAGL"
     gene            958..2181
                     /gene="hutI"
                     /locus_tag="VIW27_12455"
     CDS             958..2181
                     /gene="hutI"
                     /locus_tag="VIW27_12455"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY5740530.1"
                     /translation="MSQTLFTNATLATMQGETGYGLRQQAALLVDGDRIAWLGAMAEL
                     PEPAGAEIVDCEQRLLTPGLIDCHTHLVYGGDRADEFELRLEGASYAEIAARGGGIVS
                     TVNATRQASEEQLYAEALPRIRALLAEGVTTMEIKSGYGLDTENEAKILRVAARLADE
                     LDLRVRKTFLGAHALPPEYEGRADDYIALVCDEMLPRAQREGLVDAVDVFIESIAFDL
                     EQGERMLACAQDLGLPIKAHVEQLSDLGGAAMAARRGALSVDHLEYLNPEDVVQLAAS
                     GSVAVLLPGAYYVLGENRLPPIDALREQRVAIALATDANPGSSPVHSLLLIMNMACTL
                     FRLTPAEALRGVTLNAAKALGLADDIGSLETGKQADIVVWDVTRPAMLSYRMGMNPCA
                     AVMRAGHWRDRGIFQ"
     gene            2178..>2676
                     /locus_tag="VIW27_12460"
     CDS             2178..>2676
                     /locus_tag="VIW27_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5740531.1"
                     /translation="MSAMLDSFDDISKSAPEPIYRQIKQLIERRITSGEWIAGQKLPS
                     ENDLVAALDVSRMTINRALRELTQAGLINRVHGLGSFVAERPRHASLIELQDIALEIE
                     QRGGRHRSRVLSLESVYAGRTVTELMQVADDEPLYRLRAVHYQEELPLQLESRYVNPR
                     AMPDFI"
BASE COUNT          516 a          782 c          861 g          517 t
ORIGIN      
        1 cagccctgcg atgcaaccgc tagcggccat gctgcgagtt tcggcagcgt ccatatgcgt
       61 ggcgtgaatc aggcaccagt cggaattcac gtcgaagtta tcgaacagcc aggctaccgg
      121 gcgctggcca ctccacgcga ggcaatcgtc gacctctttg gtttgctcgg cgacgtgaat
      181 atgaatcgct gctagccgat cgaggctgcc gatgacctcg ttcagcaatt cccggtcgat
      241 cgcgcgtagc gaatgcggtg caatgccgac cgaggcgtta gcgtccccga cggtggcctg
      301 ctgcaatgaa tcgacgatac cgacgaagtc gtcggtctta ttaatgaatc gtttctggcc
      361 gtcgagcggc gcgacgctac caaacccgcc gtaacgatat aaaaccggca gtgcggtaaa
      421 tccgaggccg acgctgcgcg ccgcctgcag gcattgcagg ctcatttcgg cggggttggt
      481 gtaagcctta ccagcgacat cgtggtgcag gtactggaat tcaccgacgc agctatagcc
      541 cgccttgagc atctcgaggt agagctgtgc cgcgatgtga tgcagatgat ccggctggat
      601 gcgctcgagg tagtggtaca tgaccttgcg ccaggtccag aagctgtctt cgttgttgcc
      661 ggcgcgctcg ccgagtcccg ccatcgcgcg ctggtgcgcg tgcgagtgca cgttcggcac
      721 cgccggaatc agcacttcgg cccgggcgcc cgaaatgtag tccgaatccg gcgcaacggc
      781 ttcgatactg ccgtcttcag cgatacttat ttcgaccgag tctgcccagc cggtggccag
      841 cagcgcggag gcagcccaga tttttgtcgt catgctcaga tccgtgaatc ctgttgcgtt
      901 tgcggagaaa gcagcgtagc atacttgtat atacaagtat aggtcacagc aggactaatg
      961 tctcaaactc tgttcaccaa cgcaaccctg gcaacgatgc agggcgagac cggctacggg
     1021 ctgcgtcagc aggccgcgtt gctggtcgat ggcgatcgaa tcgcctggct cggagccatg
     1081 gcggagctgc cagagccggc cggcgccgag attgtcgatt gcgagcagcg cctgctgacc
     1141 ccgggactga tcgattgtca tacccatctg gtttacggtg gagaccgcgc cgatgaattc
     1201 gagttgaggc tagagggcgc aagctatgcc gagatcgcgg cgcgtggtgg tggcatcgta
     1261 tcgacggtca atgcgacccg ccaggccagc gaagaacaac tgtatgccga agccttgccg
     1321 cgcatcaggg ccctgttggc cgaaggcgtg accacgatgg aaatcaagtc cggttacggg
     1381 ctcgacaccg agaatgaggc caagatactg cgcgttgcgg ccaggcttgc cgatgagctc
     1441 gacctgcggg tgcgcaagac cttcctcggt gcccacgccc tgccgccaga atacgagggg
     1501 cgtgccgacg actatattgc gttggtgtgt gatgaaatgc tgccacgggc gcagcgcgaa
     1561 ggattggtgg atgcggtcga cgtattcatc gagtcgatcg ccttcgatct cgagcagggc
     1621 gagaggatgc tcgcctgcgc acaggacctg ggcctgccga taaaggcgca tgtcgaacag
     1681 ctgagcgatc tgggtggtgc cgcgatggct gcccggcgcg gtgcgctgtc ggtcgaccac
     1741 ctggagtacc tgaatcccga agatgtcgta cagctcgccg ccagcggcag cgttgcggtg
     1801 ttattgcccg gcgcctacta cgttctgggc gaaaaccggc taccgccgat cgacgcattg
     1861 cgcgagcagc gggtagcaat cgcgctggcc accgacgcta acccgggcag ctcgccggtg
     1921 cattcactgc tgttgatcat gaacatggcc tgtacattgt ttcgcttgac cccggccgaa
     1981 gcgctgcgcg gcgtcacgct caacgctgcg aaagcgctcg gcctcgcgga cgatatcggt
     2041 tcgctcgaaa ctggcaagca ggccgacatc gtggtctggg acgttacccg gccggcaatg
     2101 ctgagctatc ggatgggtat gaatccctgc gcggcggtga tgcgggctgg gcactggcgg
     2161 gatcgaggga tcttccaatg agtgcgatgc tcgatagttt cgatgatatc agcaagtcgg
     2221 cgccagaacc gatctatcgc cagatcaagc agttgatcga acggcgtatc acgagcggtg
     2281 aatggatcgc cgggcagaag ttgccgtcgg aaaacgacct ggtcgccgcg ctcgatgtca
     2341 gccgcatgac gattaaccgc gcgttgcgcg agctgaccca ggcgggcttg atcaatcgcg
     2401 tgcacgggct cggctctttc gtcgccgagc gaccgcggca tgcaagcctg atcgagctgc
     2461 aggacattgc gctcgagatc gaacagcgtg gtggccgaca tcggtccaga gtgctgagcc
     2521 tggagtcggt ttacgccggt cgcactgtaa ccgaactgat gcaggttgca gacgatgaac
     2581 ctctttatcg actgcgcgcg gtgcattacc aggaagagct gccactgcag ctcgaatcgc
     2641 gctacgtgaa tccgcgggcg atgcccgatt tcatcc
//
LOCUS       DATCZV010000552        11825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_204094, whole genome shotgun sequence.
ACCESSION   DATCZV010000552 DATCZV010000000
VERSION     DATCZV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204094"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VIW27_12465"
     CDS             complement(<1..396)
                     /locus_tag="VIW27_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5740532.1"
                     /translation="MTPREISLELDNHIVGQQDAKRAVAIALRNRWRRQQVAEDLRNE
                     ITPKNILMMGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVESIVRDLVD
                     VSIKQMREAEVSRVRHRAEDAAEEKVLDAL"
     gene            complement(434..973)
                     /gene="hslV"
                     /locus_tag="VIW27_12470"
     CDS             complement(434..973)
                     /gene="hslV"
                     /locus_tag="VIW27_12470"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY5740533.1"
                     /translation="MEQFEGTTILSVRRGNSVVIGGDGQVSMGNTIMKGNARKVRRLY
                     KDRVIAGFAGGTADAFLLFELFEGKLDKHGGQMVRAAVELAKEWRSDRALRRLEALLC
                     VANEEASLIISGNGDVIEPEDSLMAVGSGGPYAQAAARAMLDTTELSAREIVEKGLGI
                     AGDICVFTNHNHNFESIDY"
     gene            complement(1214..2110)
                     /gene="xerC"
                     /locus_tag="VIW27_12475"
     CDS             complement(1214..2110)
                     /gene="xerC"
                     /locus_tag="VIW27_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY5740534.1"
                     /translation="MTEDLDQFIQTLRSEKYYSPHTCSNYRRDLERFRAFLEKQQIGD
                     WQQVDYNHVSAFAAGRHRQGRKSRTIQRELSSIRSFFQFLIRHGRAQQNPAREVQAPR
                     ADRPLPKTCDAESIDRLLRVLDASDGLQLRDSAIFELIYSSGLRLAELVAIDLDDIDL
                     AQRHLVVTGKGRKTRHLPVGSKAAAAVERWLKRRPDYCRDPQQTALFVSRRGSRISPR
                     NVQSRLDLLIRRQALEQHLSPHMLRHSFATHLLESSSDLRAVQELLGHVNIATTQIYT
                     HLDFQHLAQAYDKAHPRARRKS"
     gene            complement(2116..2820)
                     /locus_tag="VIW27_12480"
     CDS             complement(2116..2820)
                     /locus_tag="VIW27_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY5740535.1"
                     /translation="MSEVSVETRQSESIDEAQVIRYLQENPEVLLAYPEIFSSLAIPH
                     RTGGATSLVERQLKLLRENNKALKSKIEELVAIARENEELNQRFHRLALELMNTDQLH
                     DVLSLVQDQVQTFFYTDYVCFRFLPGVSDAGNVLDGMYLDPQSGVIDQVMPWIEARKP
                     VCGRRNEMLNRQLFGTEVQIGSCAFIPLYHARNLGLLCLGSVSVERFGLTMGTIFLQQ
                     LGELVSNRLKNMLQVA"
     gene            complement(2813..3646)
                     /gene="dapF"
                     /locus_tag="VIW27_12485"
     CDS             complement(2813..3646)
                     /gene="dapF"
                     /locus_tag="VIW27_12485"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY5740536.1"
                     /translation="MKLKFAKMHGLGNDFMVVDAINQRFQPNPELVRRWADRFDGIGF
                     DQLLVVEPPAGDDAAFRYRIFNADGGEVAQCGNGARCFARFVREQGLTDLDSIPVETN
                     TGLLRIDAVDETRFRVDMGVPRFEPAEIPLRADARQPLYTAEFEATEISFSAVSVGNP
                     HMVIQVPDVDTAPVETLGPYFERHMIFPERANIGFMQVVDRGRFRLRVFERGVGETRA
                     CGSGACAAMVCGVQLDLLDSSASAELRGGELILQWQGDANPVMMTGDTAMVYQGEIEY
                     E"
     gene            complement(3643..4890)
                     /gene="lysA"
                     /locus_tag="VIW27_12490"
     CDS             complement(3643..4890)
                     /gene="lysA"
                     /locus_tag="VIW27_12490"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY5740537.1"
                     /translation="MDYFEYRQGALHCEAIAVSELAREYGTPLYVYSRATLERHWHAF
                     DRALADHRHLVCYAVKANSNLAVLNLLARLGSGFDIVSGGELRRVLAAGGDPRRIVFS
                     GVGKQSHEIREALDADILCFNVESEGELDRLQQIAAARGRRARISFRVNPDVDARTHP
                     YISTGLKENKFGVAFAEAETVYRKAAELEHIEIVGMDCHIGSQLTEMSPYIDALDRLL
                     ELIARLSEAGIEISHLDLGGGLGIRYRDEQPPLPADWASALRERLASFDGTIIIEPGR
                     AIAGNAGILVTRVDYLKHGADKNFAVVDAAMNDLIRPSLYGAWQEIIRVEQASPADER
                     VYDVVGAICESSDFLGKERALRLAQGDLLAVRSAGAYGFGMASNYNSRRRAAEIMVDG
                     DAHYCVRAREDYVDLLRGESMLP"
     gene            complement(4893..5024)
                     /locus_tag="VIW27_12495"
     CDS             complement(4893..5024)
                     /locus_tag="VIW27_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY5740538.1"
                     /translation="MKSRRIAVWLLSCCLLLALSGCGNKGPLELPDEEDERQKTRSY"
     gene            complement(5021..5659)
                     /locus_tag="VIW27_12500"
     CDS             complement(5021..5659)
                     /locus_tag="VIW27_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY5740539.1"
                     /translation="MPLELWLAYAATSAVVLAIPGPTILLVLSYSINHGRSAALPLVA
                     GVALGDTVAITLSLVGLGTLLAASAFWFTIIKWLGGLYLIYLGIQLLRSAGDPPPAQA
                     GQVGEVSPRRLFVNAFVVTALNPKSIVFFIALLPQFISAAHPLMPQLWILGTTFVGLA
                     AIGATAYALFAASIRRFLSTPRAQKIYSFAGGGLLGGAGIWALTAKRVVTAS"
     gene            complement(5665..6150)
                     /locus_tag="VIW27_12505"
     CDS             complement(5665..6150)
                     /locus_tag="VIW27_12505"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEY5740540.1"
                     /translation="MAAPTITEIKHTLAGERKTFDCELLQRSASEVVVIYRMPRDVQL
                     EDILLRRGTVSLGYFWADRPYNAYHWVDQRLDSVALYFNISDRTHIGEQSIEWRDLAV
                     DILISPDGRCRVLDEDELPADIDADLRAYIDAARDELCRQPLSRLGDYDKLTRSLMQR
                     G"
     gene            complement(6152..6553)
                     /locus_tag="VIW27_12510"
     CDS             complement(6152..6553)
                     /locus_tag="VIW27_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020039600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEY5740541.1"
                     /translation="MRIDIDGTLFDALLEQRKSPRTCAAFSAVMPFESRIVHVRWSGE
                     GVWIPLGDTDFAVGFEDATAYPAPGQILLYPGGVSETEILIAYGSVQFASKAGVLAGN
                     HFATITGDLERLREIGVASLWEGAKPIRFEA"
     gene            complement(6558..7409)
                     /locus_tag="VIW27_12515"
     CDS             complement(6558..7409)
                     /locus_tag="VIW27_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY5740542.1"
                     /translation="MIPGLLRIAAFCDGERGGNPAGVSIAGRHPDETEMRRIAAEVGY
                     SETAFAMPCAGARNDAWRVRYFSPESEVPFCGHATIALGAALAGRYGDGVFRLQLNQA
                     EITVEGWREHGTTHAALQSPPTRSAPLPDALLAETLELFALRHDDLETEIPAARIHGG
                     ADHFALCLKTRALLAAMQYDLDAGRRLMNRESLITILLGWAETPRRFHTRNPFASGGV
                     YEDPATGTATAAFGGYLRDLGWPHGGSIEVLQGEDMGAPSRLWAEIPPAAGESIRVSG
                     ATRPLEA"
     gene            complement(7406..7768)
                     /locus_tag="VIW27_12520"
     CDS             complement(7406..7768)
                     /locus_tag="VIW27_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEY5740543.1"
                     /translation="MIGLLADLVVLVIAIQHFAFMVLEMFLWDKPIGLLVFQHGKVQA
                     AASKLVASNLGLYNGFLAAGLVWGLAPSVHANDIRTFFLCCIIVAGAYGAFSTRQKMI
                     AYVQSAPAAFALLLVLLS"
     gene            7898..8953
                     /locus_tag="VIW27_12525"
     CDS             7898..8953
                     /locus_tag="VIW27_12525"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEY5740544.1"
                     /translation="MPATIPVIDLAPWFAGSPPQRRELCRAIGRIGHRTGFFYIVNHD
                     IPDAQTAEYLAALKAFFALPRADKEAIDKRHSPQFRGWEAFGSELTNNRVDDREQIDI
                     GVDRAAISNPDPYYLALIGPNQWPAPELLPGFRDIVDDYFSRLANLSRQVLRMMSLSL
                     GLDEMHIERVFGANPSPYLKMIRYPRTRTGGQGVGVHKDSGFLTLLLQDAQPGLEAQA
                     NDDSWYRVDPLPGSLVVNTGELLQLVTHNYFIATPHRVVNRGDQARYSSAFFYSPDLG
                     TRLDPLPVDPALIASASRSPRHRDEGLMASRAELLAGVGGMASHARPEVFGERYWQRW
                     VRSYPEIARSFYPHRVD"
     gene            complement(8950..9348)
                     /locus_tag="VIW27_12530"
     CDS             complement(8950..9348)
                     /locus_tag="VIW27_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEY5740545.1"
                     /translation="MMPNATLYRVAADAVLMLHAVFVIFVVVGLLLVLIGRWRGWGWV
                     HHFGFRLVHLLAICVVALQAWLGAVCPLTSWEMSLRARAGDATYTGAFVAHWVEQLLY
                     YRAPDWVFVALYTAFGALVAASWIWVRPRR"
     gene            complement(9360..9863)
                     /locus_tag="VIW27_12535"
     CDS             complement(9360..9863)
                     /locus_tag="VIW27_12535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY5740546.1"
                     /translation="MRIRPHTAADDGAVRQLNETVFPTPAEADLVERLRATAEPVISL
                     VAEDDGAIIGHILFSPAAFAADRGFLALALGPMAVLPGRQREGIGSALLRIGLDECSR
                     LGCRAVFVLGHADYYPRFGFVPASRFGIHSQFEVPDEVFMALELAPGALAGKAGAMRY
                     HEAFDAL"
     gene            complement(10043..10507)
                     /locus_tag="VIW27_12540"
     CDS             complement(10043..10507)
                     /locus_tag="VIW27_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5740547.1"
                     /translation="MDLELLAAKSADIDRILPLVAAYHAFEGIASDAAQRRSAVERLL
                     ADRTLGGIWLVLADGELAGYIALCRGFSIEFDGFDAFIDEFYLEAEFRSRGIGRIVLT
                     ALGREARGCGINALHLEVARDNQRARRLYEQAGFAARDRYLLMTAVLDERED"
     gene            complement(10507..11001)
                     /locus_tag="VIW27_12545"
     CDS             complement(10507..11001)
                     /locus_tag="VIW27_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740548.1"
                     /translation="MLAIESDGNLLRIHYRSYRLGLLLLAIPPALFYELGGRLLAGGL
                     DAGERIGLAIGVLLPIAGAYLLIEFSRFSFSRDARLFRWQWRNLVKRSAGEVPFERIA
                     RVGRQAIDASDSSGRQHLYRLVVELDDGRVIPLSRGFSGLHDRKLAQIGEQVRAHLGH
                     ITVP"
     gene            complement(11076..11765)
                     /locus_tag="VIW27_12550"
     CDS             complement(11076..11765)
                     /locus_tag="VIW27_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5740549.1"
                     /translation="MNDTTEPTDGCPPVPEQDSREPPTEVLLFDAGGVLIDLGDEPLP
                     GVKVRGLAAWLRSPIALAFEKGLTTADEFAAAWIDGIGLDCDAATLIEHFRRWPIGPF
                     AGVPTLLSRLAGDYRIAILTNTNELHWPRFTDEFELDRYAERIFASHQLGMAKPEAEI
                     FHLVVDELGTAPNRILFVDDNLANVDAARAIGLRAERVGGFDELLRTLAALAIIDAAD
                     IVAIESAAATV"
BASE COUNT         2140 a         3775 c         3866 g         2044 t
ORIGIN      
        1 cagcgcgtcc agcacttttt cctcggcggc gtcctcggcg cggtgccgta cgcggctgac
       61 ctctgcctcg cgcatctgct tgatcgacac gtcgaccagg tcgcgaacga tcgattcgac
      121 atcgcggccg acgtagccga cctcggtaaa cttggtcgct tcgaccttga tgaacggcgc
      181 gttagcgagc cgcgccaggc ggcgcgcgat ctcggtcttg ccgactccgg tagggcccat
      241 catcaggata ttcttgggcg taatttcgtt gcgcaaatct tcggccacct gttgtcggcg
      301 ccagcggttg cgcagtgcga tggcgaccgc gcgcttggcg tcctgctggc cgacgatatg
      361 gttgtccagc tccagcgaaa tttcgcgggg cgtcatcggg gactgcacga tgtatttggt
      421 atccacttga ttgtcagtag tcgatcgatt cgaagttatg attgtggttg gtaaagacgc
      481 agatgtcgcc ggcgatgcct agtccctttt cgacgatttc gcgggcactc aattcggtgg
      541 tgtcgagcat cgcgcgcgcc gcggcctgcg catagggacc gccggagccg accgccatca
      601 ggctgtcctc gggctcgatg acgtcaccgt tgccggagat gatcagcgag gcttcctcgt
      661 tggcgacgca tagtagtgcc tcgaggcggc gcaacgcacg gtccgagcgc cattccttgg
      721 ccagctcgac ggcggcgcga accatttgcc cgccatgctt gtcgagctta ccctcgaaca
      781 gctcgaacag caggaaggcg tcggcggtgc cgccggcaaa gccggcgatg acgcggtcct
      841 tgtagagccg ccgtaccttg cgtgcgttac ccttcatgat cgtgttgccc atgctgacct
      901 ggccgtcgcc gccgatgacg acgctgttac cacggcgcac ggagaggatc gttgtgcctt
      961 caaattgttc caagatctga atcccggcgg agtgtctgcg aacattgtca tatatgggca
     1021 gcggaaaata aagcaaggtc gaagcagcta tgctgcttcg accgcagtcc ccgcgaaagc
     1081 ggggacctcc ccggtatcac ggcgccgcgc ggaccctggg tgagcgggaa cttcgtaatg
     1141 acggcgtgat ttcgagattc ccgcgggtcc gcgggccgca tgcgctaacc gccccaaact
     1201 ccgcaaggga gagtcaggat ttgcggcgcg ctcgcggatg ggccttgtcg taggcctgcg
     1261 cgaggtgctg aaagtcgaga tgggtataaa tttgcgtggt ggcgatattg acgtggccga
     1321 gcaattcctg caccgcgcgc agatccgagc tggactccag caagtgggtc gcgaacgaat
     1381 gccgcagcat gtgcggcgac aggtgctgtt ccagcgcctg gcggcgaatc agcaagtcga
     1441 gccggctctg tacgttgcgc ggtgagatgc gcgagccccg gcggctgacg aacagcgcgg
     1501 tctgctgcgg gtcgcgacag taatccgggc gccgtttcag ccagcgctcg accgcggccg
     1561 cggccttgct gccgaccggc aggtgacgcg tcttgcggcc cttgccggtt actaccaggt
     1621 gccgctgcgc gagatcgatg tcgtcgaggt caatagcgac cagttcggcg agccgtagcc
     1681 ccgacgagta aatcaattcg aagatcgcgc tatcgcgcaa ctgcaggccg tcgctggcgt
     1741 cgagcacccg taacagccga tcgatgcttt ccgcgtcgca agtcttcggc agcggccggt
     1801 cggcgcgcgg cgcctgcact tcgcgcgccg gattctgctg cgcgcggccg tggcgtatca
     1861 ggaactggaa aaaactgcga atcgaggaca gttcgcgctg aatggtgcgg ctcttgcggc
     1921 cctggcggtg gcgcccggcg gcaaacgcac tgacgtggtt gtagtcgacc tgctgccagt
     1981 cgccgatctg ctgtttctcg aggaacgctc gaaagcgctc gaggtcgcgg cggtagttgc
     2041 tgcaggtgtg cggggaatag tatttttcgc tgcgcagcgt ctggatgaac tggtccagat
     2101 cctcggtcac cgcgatcagg ccacctgcag catgttcttc agccgattac tgaccagttc
     2161 accgagttgc tgcaggaaaa tcgtccccat ggtcagcccg aagcgctcga cgctgacgct
     2221 gccgaggcac agcaggccga ggttgcgcgc atggtaaagc ggaatgaagg cgcaggaccc
     2281 gatctgcacc tcggtgccga acaactggcg attgagcatc tcgttgcgcc gcccgcatac
     2341 cggtttgcgt gcttcgatcc acggcatcac ttggtcgata acgccgctct gcggatccag
     2401 gtacatgccg tcgagcacgt tgccggcgtc gctgaccccc ggcaggaaac gaaagcagac
     2461 gtaatcggtg tagaaaaagg tctgtacctg gtcctgcacc agcgacagta cgtcgtgcag
     2521 ctggtcggta ttcatcaact ccagcgccag ccggtgaaaa cgctggttta actcttcgtt
     2581 ttcgcgcgcg atggcgacca gctcctcgat tttggatttc agcgccttgt tgttctcgcg
     2641 cagcagcttg agttggcgct cgaccagcga agttgcgccg ccggtgcgat gcggaatcgc
     2701 cagcgacgag aaaatctccg ggtaggcgag cagcacctcg ggattttcct gcaggtagcg
     2761 aatcacctgc gcctcgtcga tggattcgga ttgccgtgtt tcgaccgata cttcactcat
     2821 actcgatttc gccctggtaa accattgccg tgtcgcccgt catcataacg gggtttgcgt
     2881 cgccttgcca ttgcagaatg agttcgccac cgcgcagctc cgcgctggcg ctcgagtcga
     2941 gcaggtcgag ctgtacgccg cagaccattg ccgcgcaggc gccgctgccg caggcgcggg
     3001 tctcgccgac gccgcgttcg aaaacgcgca gccgaaaacg cccgcggtcg acgacttgca
     3061 tgaagccgat gttggcgcgc tcgggaaata tcatatgccg ctcgaaatat ggcccgagtg
     3121 tttccaccgg cgcggtgtcg acatcgggca cctggatcac catgtgcggg ttgccgaccg
     3181 agaccgcgct gaaactgatc tcggtagcct cgaactcggc ggtgtacagc ggctggcggg
     3241 cgtcggcgcg cagcgggatc tcggccggct cgaagcgcgg cacgcccatg tcgacgcgaa
     3301 agcgcgtctc atcgaccgcg tcgatcctca acaagccggt attggtctcc accgggatcg
     3361 agtcgaggtc ggtcaggccc tgctcgcgca cgaagcgtgc aaagcagcgc gcgccgttgc
     3421 cacactgtgc gacttcgccg ccgtcggcgt tgaagattcg gtacctgaat gcggcgtcgt
     3481 cgccagcggg cggctcgacc accagcagtt ggtcgaagcc gatgccgtcg aagcggtccg
     3541 cccaccggcg caccagctcg ggattcggct ggaagcgctg gttgatcgcg tcgaccacca
     3601 tgaagtcgtt gcccagcccg tgcatcttgg cgaacttcag cttcacggca gcatcgattc
     3661 gccgcgcagc aggtcgacgt aatcctcgcg cgcgcgcacg cagtaatgtg catcgccgtc
     3721 gaccatgatc tcggcggcgc gccgccggct gttgtaattc gacgccatgc cgaagccgta
     3781 ggcaccagcc gagcgaaccg ccagcaaatc cccttgcgcg agccgcagcg cgcgctcttt
     3841 gccgaggaaa tcgctggatt cgcaaatcgc accgaccacg tcatagacgc gctcgtcggc
     3901 cgggctagcc tgttcgacgc gaatgatttc ctgccaggcc ccgtacagtg acggccgaat
     3961 caggtcgttc atcgccgcat cgacgaccgc gaaattcttg tcggcaccgt gcttgagata
     4021 gtcgacgcgg gtcaccagga tgccggcatt accggcgatc gcgcgtccgg gttcgatgat
     4081 gatcgtaccg tcgaagctgg ccagccgctc gcgcagcgca ctcgcccagt cggcgggcag
     4141 cggcggttgc tcgtcacgat aacgaatgcc gagtccgcca ccgagatcga gatggctgat
     4201 ttcgatgccc gcctcgctga gccgcgcgat caattccagc aggcggtcga gcgcgtcgat
     4261 gtagggcgac atctcggtga gctgcgaacc gatgtggcaa tccatgccga cgatctcgat
     4321 atgctcgagt tcggcggcct tgcggtacac ggtctcggcc tcggcaaagg cgacgccgaa
     4381 cttgttttcc ttgagcccgg tggaaatata cgggtgggtc ctggcgtcga catcgggatt
     4441 gacgcggaac gatatacgtg cccggcggcc gcgcgccgcg gcgatctgct gaaggcgatc
     4501 gagctcgcct tcggattcga cgttgaagca caggatgtcg gcatccaggg cctccctgat
     4561 ttcgtgggac tgcttgccga ccccggagaa cacgatgcgc cgcggatcgc cgccggccgc
     4621 gagcacgcgg cgcaattcgc cgccggagac gatgtcgaaa ccggaaccga ggcgcgccag
     4681 caggttgagc accgcgaggt tggaattggc cttgaccgcg tagcagacca ggtgacgatg
     4741 gtcggcgagc gcccggtcga aggcatgcca gtggcgttcc agcgtcgccc gcgagtaaac
     4801 gtagagcggc gtgccgtact cgcgcgccaa ttcggaaacc gctatcgcct cgcaatgcag
     4861 cgcgccctgc cggtactcga aataatccac ggttagtagc tccgcgtctt ctgccgctcg
     4921 tcttcttcgt cgggcagttc cagcggcccc ttgttaccgc agcccgacaa cgccaacagc
     4981 aggcagcagc tcagcagcca gacggctatg cggcgggatt tcacgacgcg gtgaccacgc
     5041 gcttggcggt cagtgcccag atgccggcgc cgcccagcag cccgccgccg gcgaagctgt
     5101 aaattttttg cgcgcgcggc gtcgacagga agcgacggat cgatgccgcg aacagcgcgt
     5161 aggcggtggc gccgatcgcc gccagtccga cgaaggtcgt gccgagaatc cataactgcg
     5221 gcatcagcgg gtgagcggcg ctgatgaact gcggcagcag cgcgatgaag aagacgatgc
     5281 ttttcgggtt cagcgcggtg accacgaagg cattgacgaa cagcctgcgc ggcgatacct
     5341 cgccgacctg accggcctgc gccggtggcg gatcgccggc gctgcgcagc agctggatgc
     5401 cgagatagat caggtacagt ccgcccagcc atttgatgat cgtaaaccag aacgcggagg
     5461 ccgccagcag cgtgccgaga ccgaccagcg acagcgtgat ggcgaccgta tcgccgagcg
     5521 cgacgccggc caccagcggc agcgcggcgg aacggccgtg gttgatcgag tagctcagca
     5581 ccagcagaat cgtcggtccg ggaatggcga ggactaccgc gctggtggcg gcataggcaa
     5641 gccagagttc gagcggcatg cgagttatcc gcgttgcatc aatgaacgtg tgagcttgtc
     5701 ataatcgccc aggcgagaca atggctggcg gcacaattcg tcacgcgccg cgtcgatgta
     5761 ggcgcgcaag tcggcgtcga tatcggccgg caattcgtcc tcgtcgagta cccggcagcg
     5821 gccgtcgggc gaaatcagga tatcgacggc caggtcgcgc cattcgatgc tctgctcgcc
     5881 gatgtgcgtg cggtcgctga tattgaaata cagcgcgacg ctgtcgagcc gttggtcgac
     5941 ccagtgatag gcgttgtagg ggcggtcagc ccagaagtag ccgaggctca ccgtgccgcg
     6001 gcgcagcaga atatcctcga gttgcacgtc gcgcggcata cggtagatca ccaccacctc
     6061 gctggccgag cgctgcagca actcgcagtc gaaggtcttg cgttcgccgg cgagcgtgtg
     6121 cttgatttcg gtgatcgtcg gcgccgccaa ctcaggcctc gaagcgaatc ggtttggcgc
     6181 cttcccacag gctcgcgacg ccgatttcgc gcaaccgctc gaggtcgccg gtaatggtgg
     6241 cgaaatgatt gccggcaagc acgccggcct tggaggcgaa ctgtacactg ccgtaggcga
     6301 tcaggatttc ggtttcgctg acgcccccgg ggtaaagcaa tatctggccg ggcgccgggt
     6361 aagcggtcgc gtcctcgaaa ccgaccgcga agtcggtgtc gccgagcggt atccagacgc
     6421 cctcgccgct ccagcgcacg tgcacgatgc ggctctcgaa aggcatcacg gcgctgaacg
     6481 cggcgcaggt ccgcggcgat ttgcgctgct cgaggagggc atcgaaaagc gttccgtcga
     6541 tatcgatacg catctgctca ggcctccagc gggcgggtgg cgccggatac gcggatcgat
     6601 tcgcccgccg cgggcggaat ctcggcccac aggcgcgatg gtgcgcccat gtcctcgcct
     6661 tgcaggactt ctatcgagcc gccatgcggc cagccgaggt cgcgcaggta gccgccgaac
     6721 gcggcggtgg cggtgccggt ggccgggtct tcgtagacgc cgccggacgc aaacggattg
     6781 cgcgtatgaa atcgccgcgg ggtttccgcc cagccgagca ggatggtgat caggctttcg
     6841 cgattcatca ggcggcgccc ggcatccaga tcgtattgca tcgcggccag cagggcgcgc
     6901 gttttcaggc acagcgcgaa gtgatcggcg ccaccgtgaa tgcgcgcggc tggaatttcg
     6961 gtttcgagat cgtcgtgccg aagggcgaac aactcgagcg tctcggcgag cagcgcgtct
     7021 ggcagcggcg cgctgcgggt aggcggcgat tgcagcgccg catgggtcgt gccgtgttcg
     7081 cgccagccct cgacggtgat ctctgcctgg ttcagttgca gcctgaagac gccgtcgccg
     7141 taacggccgg cgagggccgc gccgagcgcg atggtcgcat ggccgcagaa cggaacctcc
     7201 gattcggggg aaaaatagcg cacccgccag gcatcgtttc gagcaccggc acagggcatc
     7261 gcgaacgcgg tctccgagta gccgacctcg gccgcgatac ggcgcatctc ggtctcgtcg
     7321 ggatgccggc cggcgatcga cacgccggcc gggttgccgc cacgctcgcc gtcgcagaaa
     7381 gcggcgatgc gcagcaggcc gggaatcacg acagcaatac cagcaacagc gcgaacgccg
     7441 ccggcgccga ctgcacgtag gcgatcatct tttgccgggt gctgaaagcg ccgtaggcgc
     7501 cggcgacgat gatgcagcag aggaagaagg tgcgaatgtc gttggcgtgc accgacggcg
     7561 ccagtcccca gaccagcccg gcggcgagga aaccgttata cagcccgagg ttcgaagcca
     7621 ccagcttgga ggccgcggcc tgtaccttgc catgctgaaa caccagcagg ccgatcggct
     7681 tgtcccacag gaacatctcc agcaccatga atgcgaaatg ctggatcgcg attaccagca
     7741 ccaccagatc ggcgagcaaa ccgatcacga tacgcacccg ccacgcgctg cgagcgacgg
     7801 cgcgggcacg cgccgcggcc gccggtcgtc aagcgagtcg attcgagtat actcggcaca
     7861 catatcacta gttttagcca ataacggccg attttcaatg ccagcgacga ttccggtcat
     7921 cgatctcgcc ccctggtttg ccggtagccc gccgcagcgc cgcgaactgt gccgggcaat
     7981 tggccgcatt gggcaccgga cgggtttctt ttatatcgtc aaccacgaca ttcccgacgc
     8041 gcagaccgcc gaatatctgg ccgcgctgaa ggcctttttc gcattgccga gagcggacaa
     8101 ggaggccatc gacaagcggc attcgccgca gtttcgcggc tgggaggcgt tcggctcgga
     8161 actcaccaac aaccgcgtcg acgaccgcga gcagatcgat atcggcgtcg atcgcgcagc
     8221 gatatcgaat cccgatccct actacctggc gctgatcggc cccaaccagt ggccggcgcc
     8281 cgaactgttg cccggctttc gcgatatcgt cgacgattac ttctcgcggc tcgcaaatct
     8341 gtctcgccag gtgctgcgca tgatgtcgct gtcactcggc cttgacgaaa tgcacatcga
     8401 acgcgtgttc ggcgccaatc cgtcgcccta tctgaaaatg attcgttatc cgcgcacccg
     8461 caccggtggc cagggcgttg gcgtgcacaa ggactcggga tttctgactc tgctgctgca
     8521 ggacgcgcag ccggggctgg aagcgcaggc caacgacgac agctggtacc gagtcgatcc
     8581 attacctggc agcctggtgg tcaacaccgg cgaactgctg caactggtga cccataatta
     8641 cttcatcgcc acaccgcacc gggtcgtcaa ccgcggcgac caggcgcgct actcgtcggc
     8701 gtttttctac agtcccgacc tcggcacgcg gctcgatccg ttgccggtcg acccggcgct
     8761 gatcgccagt gccagccgca gcccgcgcca tcgcgacgag ggtttgatgg cgtcgcgcgc
     8821 cgagctgctg gccggtgtcg gcggcatggc gagccacgcg cggccggagg tattcggtga
     8881 gagatactgg cagcgctggg tgcgctccta cccggagatc gcgcggtcct tttatccgca
     8941 tcgcgttgac tagcgccgcg ggcgcaccca gatccagctg gccgccacca gcgcgccgaa
     9001 tgccgtgtac agcgcgacga acacccagtc gggagcgcgg tagtacagca gttgctccac
     9061 ccagtgggcg acgaacgcgc ccgtgtaggt agcatcaccg gcccgcgcgc gcaggctcat
     9121 ttcccagctg gttagcgggc agaccgcgcc cagccaggct tgcagggcga cgacacagat
     9181 cgccagcagg tgcaccagcc gaaatccgaa atgatgcacc cagccccagc cccgccagcg
     9241 gccaatcagc accagcaaga ggccgacgac cacgaagatc acgaacacgg cgtgcagcat
     9301 cagtacggcg tcggcggcta cccggtaaag cgtggcgttc ggcatcatcg cgagcgccgt
     9361 tagagggcgt cgaaggcttc gtggtagcgc atagccccgg ctttgccggc gagcgcaccg
     9421 ggcgcgagtt ccagcgccat gaagacttcg tccggcacct cgaactgcga atggataccg
     9481 aaacgcgaag ctggcacgaa accgaaacgg ggatagtaat cggcgtggcc gaggacgaaa
     9541 acggcgcggc agccaagccg gctgcattcg tcgaggccga tacgcagcag ggcgctgccg
     9601 atgccctcgc gttggcggcc cggcagcacc gccatcggcc ccagcgccag cgcgaggaaa
     9661 cctctgtcgg cggcgaatgc cgccggcgaa aacaggatgt ggccgatgat cgcgccatcg
     9721 tcctcggcca ccagcgagat taccggctcg gccgtggcgc gcaaccgctc gaccagatcg
     9781 gcctcggccg gtgttggaaa cacggtctcg ttgagttgcc tcacggcgcc gtcgtcggcg
     9841 gcggtgtgcg gtcgaatgcg catcgtcttt gttctcctgc gaagccgtcg ccgactattc
     9901 tacatcgcat cgtttgcccc ggaatcggcc ggcggagcag ataaataagg tttataagca
     9961 agggaactgg ataagttctc cgaattagtc cggtgagccc gcggcagtgc gtcaggtcgc
    10021 gacgttactc tttgccagcg gctcaatcct cgcgctcgtc gagcaccgcg gtcatcagca
    10081 ggtagcggtc acgcgccgcg aaacccgcct gctcgtaaag ccggcgggcg cgctggttgt
    10141 cgcgcgcgac ctcgaggtgc aacgcgttga ttccgcaccc acgcgcctcg cggccgagcg
    10201 ccgtgagcac gatcctgccg ataccccggc tgcggaattc ggcttcgagg taaaactcgt
    10261 cgataaaggc gtcgaaaccg tcgaactcga tactaaagcc gcggcatagc gcgatatacc
    10321 cggccagctc gccgtcggcg agcaccagcc agatgccgcc gagcgtgcgg tcggcgagca
    10381 gccgctcgac cgccgagcgg cgttgcgcgg cgtcgctggc gatgccctcg aaggcgtgat
    10441 aggcggccac cagcggcagg attcggtcga tgtcggcgga tttcgcggcc agcagttcga
    10501 ggtccattac ggcaccgtga tatggccgag atgcgcgcga acctgttcgc cgatctgtgc
    10561 gagcttgcgg tcgtgcagtc cggaaaagcc gcgcgacaac ggaatcactc ggccgtcgtc
    10621 gagttccacc accaaccggt aaaggtgctg tctgccggag gagtcactgg catcgatggc
    10681 ctgccgaccg acccgtgcga tccgctcgaa cggcacctcg ccggcgctgc gcttgacgag
    10741 attgcgccat tgccagcgaa acagccgcgc gtcgcgactg aagctgaaac gcgaaaattc
    10801 gatcagtaaa taggcgccgg cgatcggcaa caacacgccg atcgcgaggc cgatacgctc
    10861 accggcatcg aggccgccgg cgagcaggcg gccgccgagt tcgtagaata gtgccggtgg
    10921 tatcgccagc aatagcagcc cgagccggta actgcgatag tgaatgcgta acaggttgcc
    10981 gtcgctctcg attgccagca tgggacttcc gggtgttgcg atatgccgca agcatagaac
    11041 gcgcaagcca cgaccgccag tcgtccgcgc cgcgattata cggtggcggc cgcggattcg
    11101 atggccacga tatcggccgc atcgatgatt gccagtgccg cgagggtgcg cagcaattcg
    11161 tcgaagccgc caacccgctc cgcccgcaga ccgatcgccc gcgcggcatc gacattcgcc
    11221 aggttatcgt cgacaaacag gatgcgattc ggcgcggtgc cgagttcgtc gaccaccaaa
    11281 tggaagattt ccgcctccgg cttggccatg ccgagctggt gcgaggcgaa gattcgctcg
    11341 gcatagcggt cgagctcgaa ttcgtcggtg aagcgcggcc agtgcagttc gttggtgttg
    11401 gtcaggatcg cgatccggta atcgccggcc agccgggaca acagcgtcgg caccccggcg
    11461 aacggcccga tcggccaacg gcggaaatgc tcgatgaggg tggcggcatc gcagtcgagg
    11521 ccgataccgt cgatccaggc ggcggcgaat tcgtcggcag tggtgagccc cttttcgaaa
    11581 gccagcgcga tcggcgagcg cagccaggcg gcgagacccc ggaccttaac gccgggcagc
    11641 ggctcgtctc cgaggtcgat cagcacgccg ccggcgtcga acagcagcac ctcggtgggc
    11701 ggctcgcggc tgtcctgctc gggcaccggc gggcagccgt cggtgggttc cgttgtgtcg
    11761 ttcatcctct tttccgtgtc gctgcggtca gccgaaatcg tgcggcgcgt cgtcgatcag
    11821 gcgct
//
LOCUS       DATCZV010000553         5653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_45495, whole genome shotgun sequence.
ACCESSION   DATCZV010000553 DATCZV010000000
VERSION     DATCZV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45495"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VIW27_12555"
     CDS             <1..174
                     /locus_tag="VIW27_12555"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY5740550.1"
                     /translation="AYLRHLDKCGEILGARLNTIHNLHFYQRLMRDMREAIEAGRFDE
                     FSANFYRRYETNL"
     gene            307..636
                     /gene="yajC"
                     /locus_tag="VIW27_12560"
     CDS             307..636
                     /gene="yajC"
                     /locus_tag="VIW27_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY5740551.1"
                     /translation="MSFFINDALAQGAGQQQGSIIELILPLLLMFGIFYFLLIRPQQK
                     KAKEHKNMVEALAKGDEVITNGGLLAKITDSDENFLTCRIADNVEVKIQRHAVASVLP
                     KGTIKNL"
     gene            899..2767
                     /gene="secD"
                     /locus_tag="VIW27_12565"
     CDS             899..2767
                     /gene="secD"
                     /locus_tag="VIW27_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY5740552.1"
                     /translation="MVNRYAPWRYFLLILIIVIGAVYATPNLYGEDPAVQITHRVELV
                     DQSEVDNIESLLQSEAIDYKSVELDGNNVLVRFRSEEFQLRAAAQIRQALDAIDSRYA
                     VALNLAPATPDWLTALNALPMYLGLDLRGGVHFLMEVDMQSAIEKSLERAAGDMRSFM
                     REDKIRYRGVQASAETVRVRFVDQAERDAARLSLEEEYRDYVFAESNDDRSWFLEIGF
                     SESALEEERRAAIEQNISTLKNRVNELGVAEPVIQRQGDDRVVVQLPGVQDTVRAKEI
                     LGATATLEFRLVHGSFTDWAAAQSSGSAPIGSRLYRQSNGDPVLLKRSVIVTGDQIVG
                     AASGIDQQSGTPDVTITMNAKGADRMAKITRDNIGKPMGVVFIEDKFELREENGEMRR
                     VRTTEAEVINVATIRDQLSKRFHITGLDSTREARDLALLLRAGALRAPMVIVEERTIG
                     PSLGQENIERGFLSVVVGFVLVLIFMAVYYKLFGLVANLALSLNLVLMVAVLSIFQAT
                     LTLPGIAGIVLTVGMAVDANVLIFERIREEIRVGNTPQAAIFAGYEKAFGTIADANIT
                     TFIAAIVLFGFGTGPIQGFAVTLMIGIVSSMFTAIFGTRAVINLIYGGKHVTRLAI"
     gene            2770..3705
                     /gene="secF"
                     /locus_tag="VIW27_12570"
     CDS             2770..3705
                     /gene="secF"
                     /locus_tag="VIW27_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY5740553.1"
                     /translation="MAGLFSRQLDFMGLRYSAMILSAVLLLASVVSLATLKLNVGIDF
                     TGGSIIEVGYPQAVELEPIRGALETGGFGDAIVQHFGSADEVLIRLVPDEQKDKAEIS
                     SEIIALLKAAAGDTDIDVRRVDFVGPQVGEELTEDGGLAVLYALIAILVYVGFRFEYR
                     FSFGAVAALIHDVVITLGVFSVLQLNFDLSVLAAILAVIGYSLNDTIVVFDRVRENFR
                     KIRKKATLEVVNISINQTISRTLMTSFTTLLVLFSLFFLGGEVIHAFATALIIGVVVG
                     TYSSIYVATSSALALGISRSDLLLPEKEGEDMARP"
     gene            3721..3966
                     /locus_tag="VIW27_12575"
     CDS             3721..3966
                     /locus_tag="VIW27_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02647 family protein"
                     /protein_id="HEY5740554.1"
                     /translation="MHIDSELIDELTLLRRFSMGGPVAMDVHDNPDPAIVAAAERMHQ
                     KGLITEPDGGQLTDSGREAVEHMERLFNLLDPPLEPI"
     gene            4428..>5653
                     /locus_tag="VIW27_12580"
     CDS             4428..>5653
                     /locus_tag="VIW27_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5740555.1"
                     /translation="MEISGNLDSNEVLVVDSNYTRGHSFLAVLGFIKVEAKLITPEQL
                     PDIPRRELFNYLAIFSIGGEDISRFFLERNSEEQCQYPVILLADRWELSSREIVVQKP
                     FISGLCFQSDYYSLAQVLDQARKHNKRERRMHNRDCPSLVGASNPIQRISLMIDQVAD
                     TAASVLIIGDAGTGKEIIARNIHAQSSRSNKPFIPVNCGASPPAMLESEFFGQERDAL
                     GDGGRARQGYFELAEGGVIFLDEIGEMPLSLQAKLLRVLQDHRYERVGGNRSMKSDVR
                     VIAATSRRLEDLITQGRFREDLFYRLNVFPIEVPPLRERRSDVPLLIQEMIARIEREN
                     RGSVRLSEKAVAVLQQYDWPGNVRELANLIERLAILFPHGVVDSRDLPERYQSRAPSS
                     AAEQHALPNQVSPKSSA"
BASE COUNT         1117 a         1676 c         1725 g         1135 t
ORIGIN      
        1 gcctacctgc gccacctcga caagtgcggc gagattctcg gtgcgcgcct gaataccatt
       61 cataacctgc atttctacca gcgactgatg cgggacatgc gcgaggccat cgaggcggga
      121 cgattcgatg aattcagtgc gaacttttac cggcgctacg agaccaatct ctgacccggg
      181 cggcggcggc gggcgcggtg ccgaaatgtc gctttcacct tgtgggatgg tcggcctgat
      241 gccataatac gcgccgcttc ggggccgggc cccggtcaaa aatcgaaaat caggggttta
      301 ctaaagatga gcttcttcat aaacgacgcg ctagcgcagg gggccggcca gcagcagggc
      361 agcattatcg agctgattct gccgttgctg ttgatgttcg gtattttcta cttcctgctg
      421 atacgcccgc agcaaaagaa agccaaggaa cacaagaaca tggtcgaagc actcgccaag
      481 ggcgacgagg tgattaccaa cggcggcctg ctggcgaaga tcaccgacag cgatgagaat
      541 ttcctcactt gccgtatcgc cgacaacgtc gaggtcaaga ttcagcgcca cgccgtcgcc
      601 tcggtattgc ccaagggcac gatcaagaac ctgtagcctg gataaagtat cgctcggtcc
      661 cgccgttagc gagaccgatt aagattcgtc agattcgaat aaatgggaaa agatatcttg
      721 atagcagcta gcgtaagcgg cccgcaaatc gtgccggcgg caagtcgccc gactgttcgc
      781 gggcgcggcc tttcatcgag ccgtggccct ggctgtggcg gtcaattcac cccggcgccc
      841 gaatgcagcg atcctgcctc gtggctcggt aaatccttgc attacgcggg ctaacgcgat
      901 ggtcaaccgc tacgccccct ggagatactt tctcctcatc ctgatcatcg tcatcggtgc
      961 cgtttacgcg acgcccaatc tctacggcga ggatccggcg gtgcagatca cgcatcgcgt
     1021 ggagctggtc gaccaaagcg aggtcgacaa catcgagagc ctgttgcaga gcgaggcgat
     1081 cgattacaag tcggtggaac tcgacggtaa taacgtgctg gtgcgtttcc gctccgagga
     1141 attccagctg agggcggcgg cgcagattcg ccaggcgctc gatgccatcg actcgcgcta
     1201 cgcggtggcg ctgaacctcg ccccggcaac gccggactgg ctgaccgcgc tgaacgcgct
     1261 gccgatgtac ctgggtctcg atctgcgcgg cggtgtgcac ttcctgatgg aagtcgacat
     1321 gcaatcggca atcgagaaat cgctggaacg cgcggccggc gatatgcgca gtttcatgcg
     1381 cgaagataaa atccgctacc gcggcgtgca ggccagcgcc gagaccgtgc gggttcgctt
     1441 cgtcgaccag gcggagcgcg atgccgcgcg gctgtcgctg gaggaggagt accgggatta
     1501 cgtgttcgcc gaatccaatg acgaccgcag ctggtttctc gagatcggtt tcagcgagtc
     1561 ggcgctcgag gaagaacgcc gcgcagccat cgaacaaaac atttccacgc tcaagaaccg
     1621 cgtcaacgaa ctcggcgtcg ccgagccggt aatccagcgc cagggcgacg accgcgtggt
     1681 ggtgcagttg ccgggcgtgc aggacacggt acgcgccaag gaaatcctcg gcgccaccgc
     1741 gaccctcgaa tttcgcctcg tacacggcag cttcaccgac tgggccgcgg cccagtcctc
     1801 gggttcggcg ccgatcggca gccggcttta tcggcaaagc aatggcgatc cggtgctact
     1861 caagcgcagc gtgatcgtca ctggtgacca gatcgtcggc gccgcctccg gtatcgacca
     1921 gcagagtggc actcccgacg tgacgatcac gatgaatgcc aagggcgccg atcgcatggc
     1981 caagatcacc cgcgacaata tcggcaagcc gatgggggtg gtgttcatcg aggacaagtt
     2041 cgagctgcgc gaggaaaacg gcgagatgcg gcgcgtgcgc acgaccgagg cggaggtgat
     2101 caacgtcgcc acgattcgcg atcaactcag caaacgtttt cacatcaccg ggctcgatag
     2161 cacccgcgag gcgcgcgacc tggcgctgtt gttgcgggcc ggcgcactgc gtgcgccgat
     2221 ggtaatcgtc gaggagcgca ccatcgggcc aagcctcggc caggaaaaca tcgagcgcgg
     2281 cttcctgtcg gtggtcgtcg gtttcgtgct ggtgctgatt ttcatggcgg tctactacaa
     2341 gctgttcggc ctggtcgcca acctggcgct gagcctgaac ctggtgttga tggttgcggt
     2401 cctgtcgata tttcaggcga ccctgaccct gcccggtatc gccggtatcg tgctgaccgt
     2461 cggcatggcg gtcgacgcca atgtgctgat cttcgagcgt attcgcgagg agatacgcgt
     2521 cggcaacaca ccgcaggccg ccattttcgc cggttacgag aaggcgttcg gcaccatcgc
     2581 cgatgccaat atcaccacgt tcatcgccgc catcgtgctg ttcggcttcg gtaccgggcc
     2641 gatccagggc ttcgcggtga ccttgatgat cggcatcgtc tcttcgatgt ttaccgcgat
     2701 tttcggcacg cgcgcggtaa tcaacctgat ttacggcggt aaacacgtga cgcgactggc
     2761 gatctgatta tggcgggatt attctccaga caactggatt tcatggggct tcgctactcg
     2821 gcgatgatac tgtcggcggt attgctgctg gcctcggtcg tcagcctggc gacgctgaaa
     2881 ctgaatgtcg gcatcgactt caccggcggc agcatcatcg aggtgggtta cccgcaggcg
     2941 gtcgaactgg agccgattcg cggcgctctg gaaaccggcg gcttcggcga cgccatcgtg
     3001 cagcatttcg gcagcgctga cgaggtattg attcgcctgg ttcccgacga gcaaaaggac
     3061 aaggccgaga tcagttccga gatcatcgcc ttgctgaaag cggcggcggg agataccgat
     3121 atcgacgtgc ggcgagtgga cttcgtcggt ccgcaggttg gcgaagagct gaccgaggat
     3181 ggcggcctgg ccgtgctcta cgcgctgatc gcgatactcg tatacgtcgg ctttcgcttc
     3241 gagtaccggt tttcgttcgg cgccgtcgcc gcactgatcc acgacgtggt gattacgctg
     3301 ggcgtgtttt ccgtactgca gctcaatttc gatctcagcg tgctggcggc gatcctggcg
     3361 gtgatcggtt actcgttgaa cgatactatc gtggtatttg accgggtgcg tgaaaacttc
     3421 cgcaagatac gcaagaaggc taccctcgaa gtcgtcaata tatcgatcaa tcagacgatt
     3481 tcgcggacct tgatgacctc gttcaccacc ttgctggtct tgttttcgtt attctttctc
     3541 ggcggcgagg tcatacacgc attcgccact gcgctgatca tcggcgtcgt cgtgggaacc
     3601 tattcttcca tctatgtcgc cacttcctcg gcgctggcgc tcggtatcag ccgcagcgat
     3661 ctgctgctgc cggaaaagga aggcgaggac atggcaaggc cctgaacccg ggaggagaat
     3721 atgcatatag attcggaact gatcgacgag ttgaccctgc tcaggcgttt cagcatgggc
     3781 ggaccggtgg cgatggacgt ccatgacaat cccgatccgg cgatcgttgc cgctgccgag
     3841 cgcatgcatc aaaaaggctt gatcaccgag cccgacggcg gacagctgac cgacagcggg
     3901 cgcgaggccg tcgagcacat ggagcgcctg ttcaacctgc tggatccgcc gctggaaccg
     3961 atctagccgg cgctacgcgg gccacggcgc cagctcgctt cgcgccgtcg ccgcgcctgc
     4021 ggtctaccgc gcggcctttc caccaagatt ccgcgaattg cccaacctgc cggtcggcag
     4081 aacgattcat ttttgccttt accctgatgc cggcaggtct gtccgtggtc gccgctatct
     4141 ttgatttttt ggcggcacta cttcggcggc ggtcgcggcc gcgcggatcg caactcgagg
     4201 cggctgcacc gatgatcgcc ctgcggcgct gatattggcg gctggcggat cgattttaac
     4261 ccttctcgaa gtgatttgga tgctcgattt tcaataaaaa aattttatcg aataatttgc
     4321 aattcgccgt caaaattccg tcaggttgaa ctagacttag tctgaaacca gggccgtcaa
     4381 aaatttggct taaccaaaag atacgattcc cagggtagat aacagcgatg gaaatcagcg
     4441 gaaatctgga cagcaacgaa gttctggttg tcgattccaa ctatacacgc ggtcattcgt
     4501 ttctcgcggt gctcggtttt atcaaggttg aagccaagct gataaccccg gagcagctgc
     4561 ccgatattcc acgccgtgag ctgttcaact acctggcgat attcagtatc ggtggcgaag
     4621 acatctcgcg cttcttcctc gagcgcaatt ccgaggaaca gtgccagtac ccggtgatcc
     4681 tgctggccga tcgctgggaa ttgagttcgc gcgagatcgt cgtgcagaaa ccgttcattt
     4741 ccgggctttg ctttcaatcg gactactatt cgcttgccca ggtgctcgac caggcgcgca
     4801 agcacaacaa gcgcgaacgg cgcatgcaca accgcgactg cccgtccctg gtcggcgcca
     4861 gcaatcccat acagcgcatc agcctgatga tcgaccaggt cgctgacacc gctgcatcgg
     4921 tgctgatcat cggcgatgcc ggtaccggca aggaaatcat cgcgcgcaat atccacgcgc
     4981 agtcgtcacg cagcaacaag cccttcattc cggtcaattg cggtgcgagc ccgccggcca
     5041 tgctggagag cgagtttttc ggccaggagc gagacgcgct gggcgatggc ggcagggcgc
     5101 gccagggtta tttcgagctt gccgagggcg gcgtgatctt tctcgacgag atcggcgaaa
     5161 tgccgcttag cctgcaggcc aagctattac gtgtattaca ggatcatcgc tacgagagag
     5221 tcggcggcaa ccgttcgatg aagagcgacg tgcgagtcat cgccgcgacc agccggcggc
     5281 tcgaggacct gatcacgcag ggcagattcc gcgaagacct tttttaccgg ctcaacgttt
     5341 ttccgatcga ggtgccgccg ctgcgcgaac gccgctccga cgttccgctg ctgatacagg
     5401 aaatgatcgc gcgcatcgag cgcgagaacc gggggtcggt gcggctcagc gagaaagcgg
     5461 tagcggtgtt gcagcagtac gactggccgg gcaacgtacg cgaactggcc aacctgatcg
     5521 aacgcctggc catcctgttc ccgcacggcg tggtcgattc gcgcgatctt cccgaaaggt
     5581 accagagccg ggccccgtcg agtgccgccg agcagcacgc gctgccgaac caggtgtcgc
     5641 ccaaatcgag cgc
//
LOCUS       DATCZV010000554         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_680423, whole genome shotgun sequence.
ACCESSION   DATCZV010000554 DATCZV010000000
VERSION     DATCZV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680423"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VIW27_12585"
     CDS             complement(<1..1505)
                     /locus_tag="VIW27_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009816455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740556.1"
                     /translation="MTDDKNVLLKIKNLRIEGYSDEKWIEIVHGVDLTLHRGEVMGLI
                     GESGAGKSTIGLAAMGFTRDGCRISEGSSIEFDGMELTTTPQSDLQSLRGARIAYVAQ
                     SAAASFNPAHKLIDQHTEAPVQYRIKKRLESQEDAMELYDRLRLPNPREIGFRYPHQV
                     SGGQLQRAMTAMAMACRPDLIIFDEPTTALDVTTQIEVLSAIRDIVDQFNTAAIYITH
                     DLAVVAQMADVIKVLLKGDEVEEADTRTMLESPKDDYTKSLWAVREFKRDQKARPDTG
                     VPLISVKNVDASYGTTPVLYDVSFDIFEGMTVAVVGESGSGKSTTARCITGLLPPTSG
                     EVLFKGDPLPADYHNRTKDQLRQAQMIYQMADTALNPKVKICDIIGRPAEFYSGLKGP
                     ALKNRVDELLDLIELEPSQYYNRYPPELSGGQKQRIGIARSLAADPTFIICDEVTSAL
                     DQLVAEGILRLLDKLQNELNLSYMFITHDLATVRSIADEVVVMKEGKVIEQG"
     gene            complement(1511..2572)
                     /locus_tag="VIW27_12590"
     CDS             complement(1511..2572)
                     /locus_tag="VIW27_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740557.1"
                     /translation="MNYFIIGFYALVIGASTLAAYYQKRTAFILLFSLTLVSFVMGIV
                     GGESMLWGVTILAGLIAFSAGVSYSFREFLIILLPEDMAKEMRTAPLTAGFGLFVIFT
                     YTIAGVFAPIIAPYGEAEVISSAFAPADENMLLGADQLGRDMFSRIVYGARNTVGLAL
                     LATVLAFLMGATAGLYAATKGGKLDQLLGRLVDVIMSIPSLIFSLLLLSIFGTNTTTL
                     IIVIAIIYSPRVFRLARSVGGNVVVMDYIEAAKLRGEGTWYLIRKEILPNSMAPLVAE
                     FGLEFCFVFLLVAGLSFLGLGIQPPTADWGSMVRENATLISFGEITPLIPAAAIALLT
                     VAVNFVVDWMLYRSSGLKE"
     gene            complement(2583..>3312)
                     /locus_tag="VIW27_12595"
     CDS             complement(2583..>3312)
                     /locus_tag="VIW27_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740558.1"
                     /translation="TGDFGRSLVSQTPVAEAIGTRFGNTLFLATYAAVIAVPISIILG
                     VLVALFRNSVFDRVANVVTLTSISSPEFFLGYILILFIAVKGGYFPAIAKIDDGMSLI
                     DKLERTFLPALTMVLVVVAHMMRMTRASIINLLASPYVEMARLKGVPAWKVIVKHTLP
                     NAWAPVINVVALNLAYLITGVVLVEVVFVYPGIGQMLVDSVTKRDFPVVQACCLIFAA
                     TFILLNLAADVGSILTNPRLRHPK"
BASE COUNT          682 a          993 c         1035 g          602 t
ORIGIN      
        1 ccctgctcga tgaccttgcc ctccttcatc accaccacct cgtcggcgat cgagcgcacc
       61 gtcgccaggt cgtgggtgat gaacatatac gacaggttga gttcgttctg cagcttgtcg
      121 agcaggcgca gaataccctc ggcgaccagc tggtccaacg cgctggtgac ctcgtcgcaa
      181 atgatgaagg tcggatcggc ggcgagtgaa cgcgcaatgc cgatacgctg tttctggccg
      241 cccgacagct ccggcgggta gcggttatag tattgcgagg gctccagttc gatcaggtcg
      301 agcagctcgt cgacgcggtt cttcagcgcc ggacccttga ggccgctgta aaactcggct
      361 ggccggccga tgatatcgca gatcttgacc ttggggttga gcgcggtatc ggccatctgg
      421 taaatcatct gcgcctggcg cagctgatcc ttggtgcgat tgtggtaatc cgccggcaac
      481 ggatcgccct tgaacagcac ctcgccgctg gtcggcggca acaggccggt gatgcagcgc
      541 gcggtggtcg atttaccgga accggattca ccgaccacgg ccacggtcat gccctcgaag
      601 atatcgaagg acacgtcgta cagcaccggg gtggtgccgt aggaggcgtc gacgttcttg
      661 accgagatca gcggcactcc ggtgtccggg cgggctttct gatcgcgctt gaattcacgc
      721 accgcccaca gcgacttggt atagtcgtcc ttggggcttt cgagcatggt ccgcgtatcg
      781 gcttcctcga cctcgtcgcc tttcagcaac accttgatca cgtcggccat ctgcgccacg
      841 acggcgaggt cgtgggtgat atagatcgcc gcggtgttga actggtcgac gatatcgcga
      901 atcgccgaca gtacctcgat ctgggtggtt acgtcgagcg cggtggtcgg ctcgtcgaaa
      961 atgatcaggt cggggcggca ggccatcgcc atcgccgtca tcgcgcgttg caactggccg
     1021 ccggatacct ggtgcgggta gcggaaaccg atttcgcgcg ggttcggcag gcgcaggcga
     1081 tcgtacagct ccatcgcgtc ttcctggctt tcgagacgtt tcttgatgcg gtactgcacc
     1141 ggcgcctcgg tatgctggtc gatcagcttg tgcgcgggat tgaatgacgc cgccgccgat
     1201 tgcgcaacgt aggcgatgcg cgcaccgcgc agcgattgca ggtcggattg cggtgtcgtg
     1261 gtcagctcca taccgtcgaa ttcgatcgac gaaccctcgg agatacggca accatcgcgg
     1321 gtgaaaccca tcgccgcgag gccgatggtc gacttgccgg ctcccgactc gccgatcagg
     1381 cccatgactt cgccccgatg cagcgtcagg tcgacgccgt gcacgatctc gatccatttc
     1441 tcgtccgagt atccctcgat acgcagattc ttgatcttaa gcaatacgtt tttatcatca
     1501 gtcatggcta ttactccttc agaccggacg agcggtaaag catccagtcg acgacaaagt
     1561 tgaccgccac ggtcagcagc gcgatcgctg cggccggaat cagcggcgtg atctcgccga
     1621 aagaaatcag cgtggcattt tcacgtacca tcgagcccca gtccgcggtc ggcggctgaa
     1681 tgccgaggcc gaggaaggac aggccggcga ccagcaggaa cacgaagcag aattcgagcc
     1741 cgaactcggc caccagcggg gccatcgagt tgggcaggat ttccttgcgg atcaggtacc
     1801 aggtgccttc tccccgaagc ttggcggctt cgatgtaatc catgaccacc acgttgccgc
     1861 ccaccgagcg cgccaggcgg aatacccgcg gcgagtagat gatggcaata acgatgatca
     1921 gcgtcgtcgt gttggtgccg aagatactca gcagcagcag cgaaaagatc agcgacggaa
     1981 tcgacatgat gacatcgacc aggcggccga gaagctggtc cagcttgccg cccttggtgg
     2041 ccgcgtagag acccgcggta gcgcccatca ggaaggcaag aacagtggcc agcagggcca
     2101 gtccgaccgt gttgcgcgcc ccgtagacga tgcggctgaa catgtcgcgg ccgagctggt
     2161 cggcgccgag cagcatgttt tcgtcggccg gcgcgaacgc cgaggagatg acctccgcct
     2221 cgccgtaggg cgcgatgatc ggcgcgaaga cgccggcgat cgtgtaggtg aagatcacga
     2281 acaggccgaa accggcggtc agtggcgcgg tgcgcatttc cttggccata tcctcgggca
     2341 gaagaatgat caggaattcc cggaacgagt aggaaacgcc cgccgagaac gcgatcaggc
     2401 cggcgagtat ggtaacgccc cacagcatcg actcgccgcc gacgatcccc atcacgaagg
     2461 agaccagggt cagcgagaac agcaggatga atgcggtgcg cttctgatag taagccgcca
     2521 gcgtcgaggc gccgatgacg agagcgtaaa atccgatgat aaaataattc atgattcagc
     2581 cctcacttcg gatgacgcag gcgcgggttg gtcaggatcg accccacgtc ggcagccagg
     2641 ttgagtaaaa tgaaggtggc cgcgaagatg aggcagcagg cctgaaccac cggaaagtcg
     2701 cgcttggtga cactgtcgac cagcatttgc ccgatgcccg ggtagacgaa caccacctcg
     2761 accagcacca cgccggtgat caggtaggcg aggttcagcg cgacgacgtt gatgaccggc
     2821 gcccaggcgt tcggcaacgt gtgcttgacg atgaccttcc acgccggaac acccttcaat
     2881 cgcgccatct ccacgtaggg cgaggccagc aggttgatga tcgaggcacg cgtcatgcgc
     2941 atcatgtgtg caactaccac cagcaccatc gtcagtgctg gcaggaatgt tcgctccagt
     3001 ttgtcgatca ggctcatgcc atcgtcgatc ttggcgatcg ccggaaaata tccacccttg
     3061 acggcgatga acaggatcag gatatagccg aggaaaaact cgggcgacga tatcgaggtc
     3121 agtgtcacca cgttggcgac gcggtcgaac accgagttac gaaacagcgc taccagcact
     3181 ccgaggatta tcgagatcgg caccgcgatc accgcggcat aggttgccag gaacagcgtg
     3241 ttgccgaagc gcgtaccgat ggcctccgca accggagtct gcgaaaccag ggagcggccg
     3301 aaatctcccg tc
//
LOCUS       DATCZV010000555         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_150715, whole genome shotgun sequence.
ACCESSION   DATCZV010000555 DATCZV010000000
VERSION     DATCZV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150715"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIW27_12600"
     CDS             <1..578
                     /locus_tag="VIW27_12600"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5740559.1"
                     /translation="LRPLPVEAIVRGYVIGSGWKDYQATGAICGIELPPGLPQAAQLP
                     QPIFTPSTKAEFGSHDVNVGFDEVVEKIGADKAQLIRDTSIRLYRLAAEYALARGIIM
                     ADTKFEFGVDDDDNVVLIDEILTPDSSRYWPADEYRTGISPPSFDKQFVRDYLETLDW
                     DKTPPAPTLPQEIIDRTREKYQQVRDILLAD"
     gene            699..1313
                     /locus_tag="VIW27_12605"
     CDS             699..1313
                     /locus_tag="VIW27_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019556301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY5740560.1"
                     /translation="MFKSIGTTLITGFITILPVVLTIYLLYWLVVTSEEIMGTALRYL
                     LPEVVYFPGMGTLAGLLMVFGVGLLMKAVLVRQLFAFGENILFHLPIIKTVYRAIRDL
                     FDFFSPKQDNFGRVVIVEIAGMEMIGFVTQEHEERLPEAFRGRDAVLVYLPMSYMIGG
                     YTLLVPRERIQPCKMSMDEAMRFALTAGITGKQVDSAPKRVKRA"
     gene            1310..2293
                     /locus_tag="VIW27_12610"
     CDS             1310..2293
                     /locus_tag="VIW27_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5740561.1"
                     /translation="MKAMILEQPGGPLRCVEMPVPQPGPGQLQVAVSACGVCRTDLHV
                     VDGDLSEPKLPIVPGHEIVGRVSAVGDRVDGFEIGDRVGIPWLGHTCGSCSYCRAQAE
                     NLCDAPGFTGYQIDGGYAEFTVADAAYCFPLPGDGDDAGLAPLLCAGLIGYRSLVLAG
                     EARRLGIYGFGAAAHILAQVAHFQQREIYAFTSPGDDAAQQFALELGAAWAGDSDRPP
                     PVELDAAIIFAPVGSLVPAALAAVRKGGSIVCGGIHMSNIPEFPYAILWGERVLRSVA
                     NLTRRDAIEFLALAPQVPVHTRVVEYPLADANRALDDLRAGRLTGAAVLRP"
     gene            complement(2306..2866)
                     /locus_tag="VIW27_12615"
     CDS             complement(2306..2866)
                     /locus_tag="VIW27_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY5740562.1"
                     /translation="MSASYSLQDYYADLRTITAETSDEDEILRRVGPLAQQVVADKSW
                     LEENHYKADEEQGFGVTLLHEEPDHSLAVFVVNWLPGRGTPPHDHGTWAVVAGIEGLE
                     RNLRFNRVDDGSRTDYAELEQKHAFDAAPGELVCMKTGGIHQVTNETDRMTLSLHTYG
                     RHINHTERSQFNLETNEKKDFKLRIE"
     gene            complement(3009..>3617)
                     /locus_tag="VIW27_12620"
     CDS             complement(3009..>3617)
                     /locus_tag="VIW27_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740563.1"
                     /translation="HVDYALRFSQIYGDLDLGLSWFKGTSRDPLLLPGIDAGGNPVLT
                     PFYEQMTQYGIDAQLIHLDWIWKLEAIRRELESGDFNAATAGFEYTFYGILDSATDLG
                     AIVEYSYDSRPKQEATILDNDVFVGSRLAFNDIQSSEILAGFFIDADNRGRSFRVEAN
                     RRIGSSWKGTLELQTFSNIADDDPLAALADDDFLRLEMAYYF"
BASE COUNT          601 a         1146 c         1166 g          704 t
ORIGIN      
        1 ggctcaggcc gctgccggtc gaagccatcg tgcgcggcta cgtgatcggt tccggctgga
       61 aggattacca ggcgaccggc gccatctgcg gcatcgagct gccacccggg ctgccacagg
      121 cggcacaact gccgcagccg atattcacgc cgtcgaccaa ggccgagttc ggcagccacg
      181 acgtcaacgt gggtttcgac gaggtcgtcg aaaagatcgg tgccgacaag gcgcagctga
      241 ttcgcgatac cagcattcgc ctctaccggc tggccgcaga gtacgcgctc gcgcgcggta
      301 tcatcatggc tgataccaag ttcgaattcg gcgtcgacga cgacgataac gtggttttga
      361 tcgatgaaat cctgacgccg gattcatcgc gctactggcc ggcggacgaa taccgcaccg
      421 gcatcagccc gcccagtttc gacaagcaat tcgtgcgcga ttatctcgag accctcgact
      481 gggacaagac gccgccggcg ccaaccctgc cgcaggaaat catcgacagg acccgcgaaa
      541 agtaccagca ggtacgcgac attctgctcg ccgattgatc ccgcccgtcc aaccggtttc
      601 gttagttgtg gtgaattgaa ttcggcgctg ccggtatagt gtcgccccgg gtattcggct
      661 gctaaaatcg gcgcagcttg ttgccgggag gcgcgatcat gttcaaatct atcggtacca
      721 ccctgatcac cggcttcatc accatcctgc cggtggtgct gacgatctac ctgctctact
      781 ggctggtggt aacgtcggag gaaatcatgg gcaccgcgtt gcgctacctg ttgcccgagg
      841 tggtgtattt tccgggcatg ggcacgctgg ccggtctgtt gatggtgttc ggggtcggcc
      901 tgctgatgaa ggccgtgttg gtgcgccagc tgttcgcctt cggcgagaat atcctttttc
      961 acctgccgat catcaagacc gtctaccggg cgattcgcga cctgttcgat ttcttttctc
     1021 ccaagcagga caatttcggc cgcgtggtta tcgtcgagat cgccggcatg gagatgatcg
     1081 gcttcgtcac ccaggagcac gaagagcgct tgccggaagc ctttcgcggc cgcgacgcgg
     1141 tgctcgtcta cctgccgatg agttacatga tcggcggcta cacgctgctg gtgccgcgcg
     1201 agcgcattca accctgcaag atgagcatgg acgaggcgat gcgcttcgcg ttgaccgccg
     1261 gcatcaccgg caagcaggtg gatagcgcgc cgaagcgggt gaaacgcgca tgaaggcgat
     1321 gatcctcgag caacccggcg gcccgctgcg gtgcgtcgag atgcctgtcc cgcagcccgg
     1381 acccgggcaa ctgcaggtcg ccgtctcggc ctgcggcgtg tgccgcaccg acctgcacgt
     1441 ggtcgacggc gacctcagcg agccgaagct gccgatcgtt cccggccacg aaatcgtcgg
     1501 ccgcgtcagc gccgttggcg accgtgtcga cggtttcgaa atcggcgacc gggtcggcat
     1561 tccgtggctc ggccatacct gcggaagctg cagctattgc cgcgcgcagg ccgaaaacct
     1621 gtgcgacgcg ccgggcttta ccggttacca gatcgatggc ggctacgccg aattcacggt
     1681 cgccgatgcg gcctactgct ttccgttgcc gggtgacggc gacgacgccg ggctcgcgcc
     1741 gttgctgtgt gccgggttga tcggctaccg ctcgctggta ctggccggcg aggctcggcg
     1801 tctcggcatc tacggctttg gcgcggcggc gcatatcctc gcccaggtgg cgcatttcca
     1861 gcagcgcgag atctatgcct tcaccagccc cggcgacgat gccgcgcagc aattcgcgct
     1921 cgaactcggc gcggcctggg ccggcgattc ggaccgcccg ccgccggtcg aactcgacgc
     1981 ggcgatcatc ttcgcgccgg tcggcagcct ggtccccgcg gcgctcgccg cagtgcgcaa
     2041 gggcggtagc atcgtctgcg gcggtataca catgtcaaat attccggaat ttccctacgc
     2101 gatcctgtgg ggggagcgcg tgctgcgctc ggtcgccaac ctgacgcgcc gcgacgcgat
     2161 cgagttcctg gcgctggccc cgcaagtgcc ggtacacacc cgggtggtcg agtacccgct
     2221 ggccgacgcc aaccgcgcgc tcgacgattt gcgcgccggc cggctcaccg gtgccgcggt
     2281 gctgcgcccc tgagacgcgg cgcagctact cgatacgcag cttgaaatcc tttttctcgt
     2341 tggtttcgag gttgaattgc gagcgctcgg tatggttgat atgccggccg taggtgtgca
     2401 gcgacagcgt catgcggtcg gtttcgttgg tgacctggtg aatgccgccg gttttcatgc
     2461 acaccagctc gccgggcgcc gcatcgaagg cgtgtttctg ctcgagttcg gcatagtcgg
     2521 tgcgtgagcc gtcgtcgacg cggttgaagc gcaggttgcg ctccaggccc tcgatgccag
     2581 cgaccaccgc ccaggtgccg tgatcgtgcg gcggcgtgcc gcgccccggc agccagttga
     2641 cgacgaatac cgcgaggcta tggtccggct cctcgtgcag taacgtaact ccgaacccct
     2701 gttcttcgtc ggccttgtag tgattttcct cgagccagga tttatcggct accacctgtt
     2761 gcgccagcgg cccgacgcgg cgcaggatct cgtcctcgtc gctggtttcg gcggtaatgg
     2821 tcctcaggtc ggcgtagtaa tcctgcaggc tgtagctggc actcatcgct ggtcgtctcc
     2881 ggttttttta cccggcttat gataacagat tgtccgctgc gaaaggcccg ccggcccggc
     2941 cgcgccgtaa accggccgca tcggcgctgg cggcgggccg gaccgggatc gatccgcgga
     3001 gatcggcttc agaagtagta agccatttcg aggcgcagga agtcgtcgtc ggccagtgcc
     3061 gcgagcgggt cgtcgtcagc gatattgctg aatgtctgca gctccagcgt gcctttccag
     3121 ctgctgccga tacgccggtt ggcctcgacg cgaaagctgc ggccacgatt atcggcatcg
     3181 atgaaaaaac ccgccaggat ctcgctcgac tggatatcgt tgaacgccag gcggctgccg
     3241 acgaagacat cgttatcgag gatcgtggcc tcctgcttgg gccggctgtc gtaggagtac
     3301 tcgacgatcg cgccgagatc ggtggccgag tcgagaatgc cataaaaagt gtattcgaag
     3361 cccgcggtcg cggcgttgaa atcgcccgac tcgagctcgc ggcgaatcgc ttccagcttc
     3421 cagatccagt cgaggtgaat cagctgcgcg tcgataccgt actgcgtcat ctgctcgtaa
     3481 aacggcgtca gcaccgggtt gccgccggcg tcgatgcccg gcagcagcag cgggtcgcga
     3541 ctggtgccct tgaaccacga caggccgagg tcgagatcgc cgtagatctg cgagaagcgc
     3601 agcgcgtaat cgacatg
//
LOCUS       DATCZV010000556         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_787091, whole genome shotgun sequence.
ACCESSION   DATCZV010000556 DATCZV010000000
VERSION     DATCZV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787091"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VIW27_12625"
     CDS             complement(<1..1616)
                     /locus_tag="VIW27_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HEY5740564.1"
                     /translation="MELPPFLLFFIGAALAGVTRGLLRAVIMLAVPALGLLLLWNTPH
                     GSHASIEVMNFELLIFEVDKLSLLFGYLFHIAALIAVIYSLHVRDTLQQTCSLLYAGS
                     ALGAVFAGDLLTLFIFWELLAISSVFLIWARRSERSYGAGIRYFAIHITSGLLLLVGA
                     IIHGYASGSLAIGEIGLDTPGGWLIFLAFGIKCAFPFLHNWLTDAYPESTPTGTVFLA
                     SFTTKVAVYALARCYPGTEMLVYIGAAMTCFPIFFAVIENDLRRVLAYSLINQLGFMV
                     TGIGIGTALAINGAVAHAFNDVIFKGLLLMSMGAVLHVTGKINGSELGGLYKTMPKTT
                     ILCIVGAASISAFPLFSGFVSKSMVMTAALNNDYDWVWLLLLFASAGVFHHAGIKIPY
                     FAFFAHDSGIRAHEPPTNMLVAMTIAAALCIGIGVYPQALYGLLPFDVAYTPYDTTHV
                     LAQLQLLFFSALAFVWLNLKGMYPPELPSVNLDAEWIYRKPLPRFANAVFDWVFAWQG
                     ILRGLAMTLIGKAITLLSGYHQGDGVFART"
     gene            complement(1616..1903)
                     /locus_tag="VIW27_12630"
     CDS             complement(1616..1903)
                     /locus_tag="VIW27_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740565.1"
                     /translation="MKDEREYFFDKPGNIRRVLRVLYAVCAVLLLLDVILHRHVVHSW
                     EWLWGFYPLYGFVGCVVLVLVAKWMRTFLMRAEDYYEGDGDAGGNPQQGGD"
     gene            complement(1900..>2730)
                     /locus_tag="VIW27_12635"
     CDS             complement(1900..>2730)
                     /locus_tag="VIW27_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5740566.1"
                     /translation="FGIGKAAVMPFHRWLPAAMVAPTPVSALLHAVAVVKAGVFTLLK
                     VCVMLFGLDTLRAIPVTEWLAYLAAASVLLAALVALRQDNLKARLAYSTVSQLGYISL
                     GALLATSAGVNGGALHIAMHAFGKITLFFCAGALLVAAHKTEVSELRGIGRRMPLTMT
                     AFFIGSLCIIGLPPTGGTWSKWTLLLGTLEAGQWLLMLALMISSLLSIAYLLPISVRA
                     FFPANGTDTAWHWGEVREAPWPSLLAMAITSLGCLALFFYPEFFYAPISAILEPGVNR
                     "
BASE COUNT          613 a          843 c          854 g          420 t
ORIGIN      
        1 caggtgcgcg cgaatacgcc gtcgccctga tgatagccgc tgagcagcgt aatcgccttg
       61 ccgatcaggg tcatggcgag tccgcgcagt atgccctgcc aggcgaacac ccagtcgaag
      121 accgcgttcg cgaagcgcgg cagcggcttg cgatagatcc attcggcatc gagattgacc
      181 gacggtagtt ccgggggata catccccttg aggttcagcc agacgaaggc cagcgcggaa
      241 aagaacagca actgcaactg cgccagtacg tgagtcgtgt cgtaaggcgt gtaggcgaca
      301 tcgaacggca gcagcccgta aagcgcctgc ggataaaccc cgatcccgat gcacagcgcc
      361 gccgcgatgg tcatcgcgac cagcatgttg gtcggtggtt cgtgcgcgcg tatgcccgag
      421 tcatgggcaa agaacgcgaa atacgggatc ttgatgccgg catggtgaaa tacgcccgcc
      481 gatgcaaaca gcagcagcag ccagacccag tcgtagtcgt tattcagagc ggctgtcatc
      541 accatcgatt tgctgacgaa accgctgaac agcggaaagg ctgagatcga ggcggcgccg
      601 acgatacaca ggatcgtggt tttcggcatg gtcttgtaca gaccgccgag ctcggagccg
      661 ttgatttttc cggtgacgtg cagcaccgct cccatcgaca tcagcagcaa ccccttgaag
      721 attacgtcgt tgaaggcgtg cgcgacggcg ccgttgatcg ccagcgcggt gccgatgcca
      781 atgcccgtga ccatgaagcc cagctggttg atcaggctgt aggccagcac gcggcgcaaa
      841 tcgttctcga taaccgcgaa gaaaatcgga aagcaggtca tcgccgcgcc gatataaacc
      901 agcatttcgg tacccgggta acagcgcgcc agcgcgtaaa ccgcgacctt ggtcgtgaac
      961 gaggccagga acacggttcc ggtcggcgtc gattcgggat aggcatcggt cagccagttg
     1021 tgcaggaacg ggaaggcaca cttgatgccg aaagcgagga agatcagcca gccgccgggg
     1081 gtgtcgaggc cgatctcgcc gatcgccagt gagccgctgg cgtagccgtg aatgatcgca
     1141 ccgaccagca gcagcaggcc ggaggtgata tggatcgcga aatagcgaat cccggcgccg
     1201 taagagcgct cgctgcgccg cgcccagatc agaaatacgg agctgatcgc cagcagttcc
     1261 cagaatatga acagggtcag caggtcgccg gcgaataccg cgcccagcgc actgccggca
     1321 tacagcaggc tgcaggtttg ctgcagcgtg tcgcgcacgt gcagcgaata gattaccgcg
     1381 atcagcgccg cgatgtgaaa caggtaaccg aacagcaggc tgagcttgtc gacctcgaaa
     1441 atcagcagct cgaaattcat cacctcgatg ctggcatgcg agccgtgtgg ggtgttccag
     1501 agcagcagca atccgagcgc gggcaccgcc agcatgatca ccgcgcgcag cagtccccgc
     1561 gtcactccgg cgagcgcagc gccgatgaaa aacagcagga acggaggcag ttccatcagt
     1621 cccccccttg ctgcgggtta ccgcccgcgt cgccgtcgcc ttcatagtag tcttccgcgc
     1681 gcatcagaaa ggtgcgcatc cacttggcga ccagcaccag caccacgcaa ccgacgaatc
     1741 cgtagagggg atagaatccc cataaccatt cccaggaatg aaccacgtgg cgatgcagga
     1801 tcacgtcgag caacagcaac accgcgcaga ccgcgtaaag cacgcgcagg acgcggcgaa
     1861 tgttgccggg cttgtcgaag aagtattccc tttcgtcttt catcggttca ctcccggttc
     1921 caggatcgcg gagatcggtg cgtagaaaaa ttccgggtaa aagaatagcg ccaggcaacc
     1981 cagcgaggtg attgccatcg ccagcagtga tggccagggt gcttccctga cctcacccca
     2041 gtgccaggca gtgtcggtac cgttggcggg aaagaaggcg cgcaccgaga tcggtagcag
     2101 gtaggcgata ctgagcagcg agctgatcat cagggccagc atcagcagcc actgcccggc
     2161 ctcgagcgtg cccagcagca gggtccactt gctccaggtg ccgccggtcg gcggcaggcc
     2221 gatgatgcac aggctgccga tgaaaaacgc cgtcatcgtc agcggcatgc gccggccgat
     2281 accacgcaat tcgctgacct cggtcttgtg cgcggcaacc agcagcgcgc cggcacagaa
     2341 aaacagcgta atcttgccga acgcgtgcat cgcgatgtgc agcgcaccgc cgtttacgcc
     2401 agcgctggtg gcgagcaagg cgccgaggct gatgtaaccg agctggctga cggtggaata
     2461 cgccagccgc gccttgaggt tatcctggcg cagcgcgacc agcgccgcca gcagcaccga
     2521 ggccgcggcc aggtaggcca gccactcggt caccggaatg gcgcgcagcg tatcgaggcc
     2581 gaacaacatg acgcagacct tgagcagcgt aaaaacgccc gccttgacca ctgccaccgc
     2641 atgcaacagc gcgctgaccg gggtcggcgc aaccatcgcg gccggcagcc agcggtgaaa
     2701 aggcatcacc gcggccttgc cgatgccgaa
//
LOCUS       DATCZV010000557         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_97742, whole genome shotgun sequence.
ACCESSION   DATCZV010000557 DATCZV010000000
VERSION     DATCZV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97742"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VIW27_12640"
     CDS             complement(<1..220)
                     /locus_tag="VIW27_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740567.1"
                     /translation="MSRVRIQFTLFSAFYSPLILTMAGGFLQREGLEFDWSVSPPGKP
                     ATESVVDGSADVVQSAPSQAFNAIVRYGM"
     gene            complement(648..2039)
                     /locus_tag="VIW27_12645"
     CDS             complement(648..2039)
                     /locus_tag="VIW27_12645"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY5740568.1"
                     /translation="MIDRRDFIKAGMLAACGGSLWLAAPRARAATDAYQLTASAGEVR
                     IEADQQQATQVMYYNQSIPGPLLRLPQGRESVIRFRNRLDEPSTVHWHGLRIENAMDG
                     VPGMTQAAVAPGEDFDYRLTPPDAGTYWYHSHTRSWIQQALGLAGVLIVDEVEPPRID
                     RDLVLAIDDWRFDEAMQMDTASFGSMHDWSHGGRLGNYLTANGKPEETFEVMSGERVR
                     LRLVNTANSRIMYLLLEEPQSSIVALDGQPVSPRVPDGGRIRLAPGQRCDLIIDLVGD
                     PGQRSRIEILVGEYAYEVARFAYGPEVRREQLLDTPIALPDNPVNRVRLPQDFVEATM
                     LMEGGAMGGMRSATYQGREMDIRELVKLGKVWAINGVVDLPEEPLLRVDRGTAVSLQI
                     INDNGWPHAMHVHGHHFVDDREPGVWRDTSLFARGEKSSLRFVADNPGKWLIHCHMTE
                     HMAAGMVTWFQVD"
     gene            complement(2052..>2871)
                     /locus_tag="VIW27_12650"
     CDS             complement(2052..>2871)
                     /locus_tag="VIW27_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEY5740569.1"
                     /translation="YSMERHAELVAFALGSDRCRSETLGVTLDGQTMDCLRFGDGGAG
                     HARIWMIARQHPGETMAEWWMEGMIGRLVDDSDPVVNAILDRADVYLVPNMNPDGSRR
                     GNLRTNAAGANLNREWAAPSAERSPEVLCVLRKIEETGLDFGLDVHGDEALPYNFIAG
                     TEGIASWNAQRQQQLDLFKTTLAGLNPDFQIERGYPANAPGKANMSYCSNALAERFGK
                     LVMTLEMPFKDASGTPDPLRGWNPERCARLAHSCLDALHLCWDRVIGGDSRAAS"
BASE COUNT          517 a          946 c          880 g          528 t
ORIGIN      
        1 gcatgccgta ccggacgatg gcattgaaag cctggctcgg cgccgactgc accacgtcgg
       61 cgctgccgtc gaccaccgat tcggtggcgg gcttgccggg cggagacacg gaccagtcga
      121 attccagccc ctcgcgttgc agaaaaccgc cggccatggt cagtatcagc ggcgagtaaa
      181 aggccgaaaa caacgtgaac tgtatcctca ctctggacat gaaaattatc tcctcgattg
      241 ttgcggggct cagccttcgt atcgggccag ccaacaactt tcccctatgt caatccaaga
      301 ccgtaacctt aaacagttag aacgcactaa tccagttgaa ggcctggcgc tagtgggcgg
      361 ctctggttca gtcggcgcga acgagccccg cccgcagatt ccaacggcat cctgagcgca
      421 ggcgcgggac aggcgcgcct ggacgccggg ctggaccgag agccatagcc aaagctatgg
      481 ctccagaaag ggccaggtcc aggcgtatcc ggacaagtct gcgccgggat cgggttcgag
      541 ccgctttaaa attcccgact cttccgacta tcccgagatc acattattac cgcagcaaac
      601 cataaaggtt ttgcgaggct cgaacccggc caatgtgatc tacgggctca atcgacctga
      661 aaccaggtga ccataccggc cgccatgtgt tcggtcatgt ggcaatggat cagccatttg
      721 cccgggttgt cggcgacgaa acgcaggctg ctcttctcgc cgcgcgcaaa caggctggta
      781 tcgcgccaca cgcccggctc gcgatcatcg acgaaatgat gcccgtgaac atgcatcgca
      841 tgcggccagc cattgtcgtt gatgatctgc aggctgaccg cggtgccgcg gtcgacgcga
      901 agcaggggtt cctcgggcag atcgacgacg ccgtttatcg cccagacctt gccgagcttg
      961 accagttcgc gtatgtccat ttcccggccc tggtaggtcg cgctgcgcat gccacccatc
     1021 gcgccgccct ccatcagcat ggtggcctcg acgaaatctt gcggcaatcg cacccggttg
     1081 accgggttgt cgggcagcgc gatcggcgta tccagcaact gttcccgcct gacctcgggg
     1141 ccgtaggcaa agcgggcgac ctcgtaggcg tattcgccga ccaggatctc gatgcgcgag
     1201 cgctggcccg ggtcgccgac caggtcgatg atcagatcgc agcgctgtcc cggagccagc
     1261 cgaatacgcc ccccatccgg cacgcggggg ctcacgggct gcccgtcgag cgcgacaatc
     1321 gacgactgcg gctcttccag cagcaggtac atgatgcgcg agttggcggt attcaccagg
     1381 cgcaggcgca cgcgctcgcc gctcatgacc tcgaaggtct cttccggctt gccgttggcg
     1441 gtcaggtaat tgccgaggcg gccgccatgc gaccagtcgt gcatcgatcc gaagctggcg
     1501 gtatccatct gcatcgcctc gtcgaagcgc cagtcatcga tggccagcac cagatcgcga
     1561 tcgatccgtg gcggttccac ctcgtcgacg atcaaaacac cggccaggcc cagcgcctgc
     1621 tgtatccatg aacgtgtgtg gctgtgatac cagtaggtgc cggcgtccgg cggtgtcagc
     1681 cggtagtcga aatcctcgcc gggggcgacc gcggcctggg tcatgcccgg tacgccgtcc
     1741 atcgcgtttt cgatacgcaa tccatgccag tgcaccgtgc tcggttcgtc gagacggttg
     1801 cgaaagcgga ttaccgactc gcggccctgc ggcagtctca gcaacggtcc tggtatcgac
     1861 tggttgtagt acatgacctg cgtcgcctgc tgctggtcgg cctcgatcct gacctcgccg
     1921 gcggacgcgg tcagctgata ggcgtccgtc gcggcccgtg cgcgtggcgc ggccagccag
     1981 aggctgccgc cacaggccgc cagcataccg gccttgataa aatcccggcg gtcgatcacc
     2041 gcccgttacc tttagctagc tgcacgagaa tctcctccaa ttacccggtc ccagcacaga
     2101 tgcagcgcat cgagacagga gtgcgccagc ctggcacagc gctcgggatt ccagccccgc
     2161 agcgggtccg gtgtgccgct ggcatccttg aaaggcatct ccagcgtcat caccagcttg
     2221 ccgaaacgct cggcgagcgc attggagcaa tagctcatgt tggccttgcc aggcgcattg
     2281 gccgggtacc cccgttcgat ctgaaagtcc gggttgagac cggccagggt cgtcttgaac
     2341 aaatccagtt gctgctggcg ttgagcgttc cagctggcaa tgccctcggt accggcaatg
     2401 aaattatacg gcagcgcttc gtcgccgtgc acgtcgaggc cgaagtcgag cccggtttcc
     2461 tcgatcttgc ggagtacgca cagcacctcc gggctgcgct cggcgctcgg cgcggcccat
     2521 tcccggttga ggttggcgcc ggcggcattg gtacgcaggt tgccgcgccg gctgccgtcc
     2581 ggattcatgt tcggcaccag gtagacgtct gccctgtcga ggatcgcatt gaccaccggg
     2641 tcgctgtcat cgaccaggcg tccgatcatg ccctccatcc accactcggc catggtttcg
     2701 ccgggatgct ggcgcgcgat catccagatt ctggcatggc ctgcgccgcc gtcgccgaat
     2761 cgcaggcaat ccatggtctg gccatccagg gtcacgccca gcgtctcgga gcggcagcga
     2821 tcgctgccga gcgcgaacgc gaccagctcg gcgtgacgct ccatcgaata g
//
LOCUS       DATCZV010000558         5631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_257345, whole genome shotgun sequence.
ACCESSION   DATCZV010000558 DATCZV010000000
VERSION     DATCZV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257345"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VIW27_12655"
     CDS             complement(<1..709)
                     /locus_tag="VIW27_12655"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5740570.1"
                     /translation="MRIEDDSKLDYKDVLIRPKRSTLGSRKDVELEREFCFRNYRSER
                     AEHYRGIPIMASNMDGVGTFAMADALAELGLFTCLVKNYEAAELAEFFNIDEPGRREN
                     VAYSTGITTQDFAKFEGVYAQTGKRLKYVCVDVANGYSERFSEFIKTLREAYPEIVII
                     AGNVVTGEMTEELILSGADIVKVGIGPGSVCTTRIQTGVGFPQLSAVIECADAAHGLG
                     GHIIADGGCACPGDLAKA"
     gene            984..1796
                     /locus_tag="VIW27_12660"
     CDS             984..1796
                     /locus_tag="VIW27_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEY5740571.1"
                     /translation="MTDTRPAQISLLIDLDGVVYQDGGLIEGAAEALDWLDSERIPHL
                     YVTNTTSRPRDSLLRKFDRLGFHARAAEIMTPVVAARQWLAEHGLRRAALFVPPATQR
                     DFGDIEPLPLNGDVPAEAVVLGDLGEAWDFKLLNCAFRLLMREPTPRLIALGMTRYWR
                     GPHGLQLDVAPFVKALEHAASCTAVVVGKPAPAFFASALASLDCDAGQALMIGDDIVG
                     DVGAAQDCGIRGIQVRTGKYRETDLDGDIEPFAVLDSIAGLPDWWSKMLAAN"
     gene            complement(1890..2321)
                     /gene="ndk"
                     /locus_tag="VIW27_12665"
     CDS             complement(1890..2321)
                     /gene="ndk"
                     /locus_tag="VIW27_12665"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018649004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY5740572.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGEIYDRFEKAGLRIVAARMEHLSA
                     EKAGEFYAVHRERPFYGELVDFMTSGPVMVQVLEGENAIARNREVMGATNPAEAAPGT
                     IRADFAKTVDENAVHGSDGADTARAEIEFFFGSDGICERTR"
     gene            2400..5018
                     /gene="pepN"
                     /locus_tag="VIW27_12670"
     CDS             2400..5018
                     /gene="pepN"
                     /locus_tag="VIW27_12670"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY5740573.1"
                     /translation="MMKTDHQQRKFLKDYRPPEFAIERTWLRFDLDASRTRVHSRLQM
                     RRRDAGSRAPLVLDGIDLKLLEVRIDGELLQTDGYRLDDESLSIDDLPASFEFECIVE
                     IDAAANTRLEGLYLSNGNFCTQCEAEGFRYITYYLDRPDVMSVFTTEMHAERGAYPML
                     LSNGNPVDSGESGERHWAIWEDPFPKPSYLFALVAGDLACIEDYFTTRSGREVTLRIF
                     TEHHNRDKCDHALRSLQKAMRWDEEVFGLEYDLDLYMIVAVDDFNMGAMENKGLNVFN
                     TRYVLANPQTATDADYLAIEGVIAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEF
                     SADMFSRAIQRIGDVRVLRNHQFGEDAGPMAHPVRPASYQEVNNFYTATVYNKGAEVV
                     RMMHCLLGADRFRAGMDLYFDRYDGQAVTTDDFRRAMQDASGVDLDRFQRWYEQAGTP
                     RIDISRDYDADQRKLTLVVRQHAGTTRGELNRPFHMPMRLALFAADGEPLPLDAAGRL
                     EWTVDITDHETRLEFEAVDENSMPSAFRGFSAPVIVNTDLEDAELAVLMVCDSDDFNR
                     WEASQQLACRIMLAMLKRDDSEWDGLQQPLLDAFASLLDNRELDRALSAEMLVLPGEK
                     YLAEMLEQADVHAIRRVREALAGALSAYCESSLLRHYHNCSTAGDYAIEPAAMARRRL
                     KNTCLSYLLRLQREEYFTLCGAQFDAADNMTDQLACLQSAVHYRHSESERIVAGFYRQ
                     WQDTPLVVDKWFSARGSSYAPNALDEIGALFEHPAYSLRNPNRARSLLAAMMANTSAF
                     HQSDGDGYAIAADRILELDRINPQIAARLANPFVHWRRLVPELGQRMKARVETMLASG
                     ELSNDLDELLTTSLED"
     gene            5028..>5631
                     /locus_tag="VIW27_12675"
     CDS             5028..>5631
                     /locus_tag="VIW27_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740574.1"
                     /translation="MRGRIDIVLPGLCDLPTGELDPTLLANGLPSLNRLLRLATPLPN
                     RAFTIDAILRAVLQLDNDEISSLPLAQACAVDADAPAERLLLVQAAHLRADLQHAVLV
                     PVRGGDSDDSDINILINDLSDFFSVDCDISAIADGQYLMRLKKLEAPSHYPHILSVLG
                     KSVNPYIEQSRGNLAWYRLVNEMQMFLHQHAINHERAARGL"
BASE COUNT         1024 a         1742 c         1786 g         1079 t
ORIGIN      
        1 aagccttggc gaggtcgccc gggcaggcgc agccgccgtc ggcgatgata tggccgccga
       61 gaccgtgcgc cgcatcggca cattcgatca ccgccgacag ttgcggaaag ccgaccccgg
      121 tctgaatgcg cgtggtgcag acgctgcccg ggccgatacc gaccttgacg atgtcggcgc
      181 cgctcaagat caattcctcg gtcatctccc cggtgaccac gttaccggcg atgatgacga
      241 tttcgggata agcctcgcgc agcgtcttga tgaattccga gaaacgctcg gagtagccgt
      301 tggcgacgtc gacgcagacg tatttcaggc gtttgccggt ttgcgcgtaa acgccctcga
      361 acttggcgaa atcctgcgtc gtgatgccgg tcgagtaagc gacgttttcg cgcctgccgg
      421 gttcgtcgat gttgaagaat tccgccagtt cagcggcttc gtagtttttc accaggcagg
      481 tgaacagccc gagttcggcg agtgcgtcgg ccatcgcgaa ggtaccgacg ccgtccatgt
      541 tggaggccat gatcggaatg ccgcgataat gctcggcgcg ctcggagcgg taattgcgga
      601 agcaaaattc gcgctccagc tcgacgtcct tgcggctgcc gagagtactg cgcttggggc
      661 ggatcagcac gtccttgtag tcgagcttgc tgtcgtcttc gattctcatc agatttcgcg
      721 ctcaggcggc gacgtcaggc caagttatca tgaaacgagt cgcgttaaca ggctgcccgc
      781 gagcatgcga ccggggtttc cccggcgagc cggggcgtct tgaatcgccg cgatgacggg
      841 cgtattctgg ttgcaactcg tccacccgcg agcgggtcct gaatacggat actcatgccg
      901 tgagtatagt cgagtccggc cagcggcgcg gtcgtcgaca aaccatggcc tgttccgttc
      961 gcggtgcagc aaatgatcga tttatgactg atacacggcc cgcgcaaatt tcgttgttga
     1021 tcgacctgga tggcgtcgtt taccaggatg gcggcttgat cgagggtgcg gccgaggcgc
     1081 tcgactggct cgatagcgag cgaatcccgc atctatatgt caccaatacg acctcgcggc
     1141 cgcgcgattc gctgctgcga aaattcgacc gtctcggttt ccacgcgcgg gccgcggaaa
     1201 tcatgactcc ggtagtggcc gccaggcaat ggctcgccga gcatggcctg cggcgcgcgg
     1261 cgctgttcgt gccgccggcg acgcaacgag atttcggcga tatcgagccg ctgccgctca
     1321 acggcgatgt gccggccgag gcggtagtgc tcggcgatct cggcgaggcc tgggatttca
     1381 aactgctcaa ctgcgcgttt cgcttgctga tgcgagaacc gacgccgcga ctgatcgcgc
     1441 tcggcatgac gcgctactgg cgcggtccgc acggcctgca actcgacgtc gcgccgttcg
     1501 tcaaggcgct ggagcatgcc gcctcgtgca cagccgtggt cgtcggcaag ccggcgccgg
     1561 catttttcgc gtcggcgctg gcgagcctgg attgcgatgc cgggcaggcg ctgatgattg
     1621 gcgacgatat cgtcggcgac gtcggtgcgg cccaggactg cggcatacgc ggcatccagg
     1681 tgcgcaccgg caagtatcgc gagaccgatc tcgacggcga tatcgaacct ttcgcggtgc
     1741 tcgactcgat cgccgggtta ccggactggt ggtcgaagat gctggcggcg aactgagcgc
     1801 cgcgctgcga agtcgctgcg gcgcgggccg caagccatgg ctacgtcgct aaccgccagg
     1861 taattcgcaa gccggcggtc ggcagccgtt tagcgcgtgc gctcgcagat accgtcgctg
     1921 ccgaagaaga actcgatctc ggcgcgcgcg gtgtcggctc cgtcggagcc gtgcacggcg
     1981 ttttcgtcga ccgttttcgc aaagtcggca cgaatggttc caggtgcggc ttcggccggg
     2041 ttggtggcgc ccatgacctc gcggttgcgc gcgattgcat tctcgccctc gaggacctgt
     2101 accatcaccg ggcccgaggt catgaagtcg accaactcgc cgtagaatgg ccgttcgcgg
     2161 tggacggcgt agaattcgcc tgccttttcg gcgctcaggt gttccatgcg cgcggcgacg
     2221 atgcgcaggc cggccttttc aaagcggtcg tagatttccc cgatcacgtt cttggcgacc
     2281 gcgtcgggct tgatgatcga aagtgtacgt tcgacagcca ttgagtgatt ctccacgatg
     2341 aaaaatgctg cgcataatac tccttagggt acacttatgc gacaaatttt cagacccgta
     2401 tgatgaaaac cgaccaccag cagcgcaagt ttctcaagga ttaccgcccg cccgaattcg
     2461 ctatcgagcg tacctggctc agattcgatc tcgacgcgtc gcgcacgcgt gtgcacagcc
     2521 gcctgcagat gcgccgcagg gatgccggca gccgcgcgcc gctggtgctc gacggcatcg
     2581 atctgaaact gctcgaagtg cggatcgatg gcgagctgct gcagacagac ggttatcgcc
     2641 tggacgatga aagcctgagt atcgacgatc tgccggcgag cttcgaattc gagtgcatcg
     2701 tcgaaatcga cgcggcggcg aatacgcggc tcgaaggcct gtacctgtcg aacggcaatt
     2761 tttgcaccca gtgcgaggcc gagggttttc gctacatcac ctattacctc gaccggcccg
     2821 acgtgatgtc ggtgtttacc accgagatgc acgccgagcg cggggcttac ccgatgctgc
     2881 tgtcgaacgg caacccggtg gacagcggcg agtcggggga acgccactgg gcaatctggg
     2941 aagacccgtt tccgaagccg tcctacctgt tcgcgctggt ggccggcgac ctggcctgta
     3001 tcgaggatta cttcaccacc cgatcggggc gtgaggtgac gctgcgcatc tttaccgagc
     3061 accacaatcg cgacaaatgc gatcatgcgc tcaggtcgct gcagaaagcc atgcgctggg
     3121 acgaggaggt tttcgggctc gagtacgatc tcgacctgta catgatcgtc gcggtcgacg
     3181 atttcaacat gggggcgatg gaaaacaagg gcctcaacgt gttcaacacc cgttacgtgc
     3241 tggccaatcc gcagaccgcg accgatgccg actacctggc gatcgagggt gtcatcgcgc
     3301 acgagtattt ccacaactgg accggcaacc gggttacctg ccgcgactgg ttccagctaa
     3361 gcctgaagga aggcctgacc gtgtttcgcg accaggaatt cagtgccgac atgttttcgc
     3421 gcgcgatcca gcgtatcggc gacgtgcgcg tgctgcgcaa ccaccagttc ggcgaggatg
     3481 ccgggccgat ggcgcacccg gtacgccccg cttcctacca ggaggtcaat aatttctaca
     3541 ccgcgacggt ttacaacaag ggtgccgagg tggtgcgcat gatgcattgc ctgctcggtg
     3601 ccgaccggtt tcgcgccggc atggacctct actttgacag gtacgacggc caggcggtga
     3661 ccaccgatga tttcaggcgt gcgatgcagg atgcttcggg tgtcgacctg gatcggtttc
     3721 agcgctggta cgaacaggcc ggcacgccgc gcatcgacat cagccgcgat tacgacgccg
     3781 accaacgcaa gctgacactg gtcgtcaggc agcatgccgg caccacccgg ggtgagctca
     3841 accgcccgtt ccacatgccg atgcggctgg cgctgttcgc agcggacggc gagccgctgc
     3901 cgctcgacgc tgccggccgc ctcgagtgga ccgtcgatat caccgatcac gagacacgcc
     3961 tcgagttcga ggcggtcgac gaaaacagca tgccgtcggc gtttcgcggc ttttcggcgc
     4021 cggtcatcgt caacaccgac ctcgaggatg cggagctggc ggtgctgatg gtttgcgaca
     4081 gcgacgattt caaccgttgg gaagccagcc agcaactggc ctgtcgcatc atgctggcga
     4141 tgctgaagcg cgacgacagc gaatgggacg gcctgcagca gccgctgctg gacgctttcg
     4201 ccagcctgct cgacaaccgc gaactcgacc gcgcactgag cgccgagatg ctggtgctgc
     4261 ccggcgagaa atacctggcc gaaatgctcg aacaggccga cgtgcacgcg atcaggcgcg
     4321 tgcgcgaggc gctcgccggg gcactgtcgg cgtattgcga gagttcgctg ttacggcatt
     4381 atcataattg cagcacggcg ggtgactacg ccatcgagcc ggccgcgatg gctcgccggc
     4441 ggctgaaaaa cacttgcctt tcctatttgt tgcgcctgca gagggaggag tatttcacgc
     4501 tctgcggtgc gcagttcgac gcggccgaca acatgaccga tcagctcgcc tgcctgcagt
     4561 cggcggtgca ttatcggcat tccgagagcg aacgtatcgt cgccggcttt taccggcagt
     4621 ggcaggatac gccgctggtg gtcgacaagt ggttcagcgc gcgcggctcg agttacgcgc
     4681 cgaatgcact cgacgagatc ggcgcactgt tcgagcaccc ggcgtatagt ctcaggaatc
     4741 cgaatcgcgc ccgttcactg ctcgccgcga tgatggccaa cacctcggct tttcaccagt
     4801 ccgatggcga cggctatgcg attgccgccg acaggattct cgaactggat cgcatcaatc
     4861 cgcagattgc cgcgcggctc gccaacccgt tcgtgcactg gcgccggctg gtgcccgagc
     4921 tcggtcaacg aatgaaggcg cgggtcgaga ccatgctggc ctcgggcgaa ctttccaacg
     4981 acctcgacga gttgctgacc accagcctcg aggattgatc gcgatccatg cgcggccgca
     5041 tcgatatcgt cctgccggga ctgtgtgacc tgccgaccgg cgaactcgat ccgacgctgc
     5101 tcgccaacgg cttgccgagc ctcaaccggc tgttgcgtct tgccacgccg ctgcccaacc
     5161 gcgcattcac catcgatgcg atcctgcgcg ctgtgctgca actcgacaac gacgagattt
     5221 cgagcctgcc gctggcgcag gcctgcgccg tcgacgccga tgcgccagcc gaacggctgc
     5281 tgctggtgca ggccgcgcac ctgcgcgccg acctgcagca tgcggtgctg gtaccggtcc
     5341 ggggcggcga tagcgacgac tcggatataa acatattaat caatgattta tccgacttct
     5401 ttagtgtgga ttgtgatatt tctgcgatcg ccgacgggca atacctgatg cgcctgaaga
     5461 agctcgaggc gccgagccac tacccgcaca tcctgtcggt gctcggcaag agcgtcaatc
     5521 cctacatcga gcagagtcgc ggcaatctgg cctggtaccg gctggtcaac gaaatgcaga
     5581 tgtttttgca ccagcacgcg atcaaccatg aacgcgctgc tcgcgggctg c
//
LOCUS       DATCZV010000559         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_521301, whole genome shotgun sequence.
ACCESSION   DATCZV010000559 DATCZV010000000
VERSION     DATCZV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521301"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VIW27_12680"
     CDS             complement(<1..740)
                     /locus_tag="VIW27_12680"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEY5740575.1"
                     /translation="MNSRTIKLSTPILIAAVAMLSGPLQAQDVPLDVDVNKMLSEKYK
                     GESYSPYAKREFPNEVLWGDSHLHTGISFDAGTAGAILLPADAYRFARGEEVVSSYGI
                     PVKLSRPLDWLAVTDHTDNMGFIIDLVAGNPDILATPEGKDWHERLKTASKDDKPKIA
                     YEIIKALMVTKTFPMEIYYDPNSKGYKSTWERIVKAAEQYNEPGRFTAMIGYEWTSTL
                     EGNNLHRNVLYRDSGEKAMQMMPYTTTPP"
     gene            complement(765..>2144)
                     /locus_tag="VIW27_12685"
     CDS             complement(765..>2144)
                     /locus_tag="VIW27_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEY5740576.1"
                     /translation="IVDAAETYNEPGRFTAFIGYEWTSVPKGFNLHRNVILRDGGVRA
                     RQVIPLTTQPPLGTTDPMDLYAWLDNYQKKTNGQAFALSHNGNLSNGWMFPVDKTYHG
                     NKVDQNYVEQRARWEPLYEMTQIKGDGEAHPFLSPDDEFADYENWDVGNLDITELKKD
                     EMLAGEYAREALKRGLMLEAKLGTNPYKFGMVGATDSHTGLATAEEENFFGKSVSVEP
                     SKDRVAHPFIKSKLGEISGDPSVASGYQGVWATDNTRAALFDAMLRKETYATTGPRIP
                     VRFFGGWDYTDNDLRSRQPAFIGYEKGVPMGGDLPPASEGKTAPTFMVYALRDPVGAN
                     LDRIQIVKGWLDQDGKTHERIYDVAVSDGRKIDEDGRTRELVGNTVDLEAANWTNTIG
                     ASELAAVWSDPEFDPKQSAFYYARVIEIPTPRWVLYDKVRFGAEVPEGATLIGQERAY
                     TSPIWYNPS"
BASE COUNT          347 a          619 c          693 g          485 t
ORIGIN      
        1 ggcggggtgg tggtatacgg catcatctgc atcgcttttt cgccgctgtc acggtaaaga
       61 acatttctat gcagattgtt gccttctaat gtggaggtcc attcataacc gatcatggcg
      121 gtaaagcgac cgggctcgtt atactgctcg gcggctttta cgatccgctc ccaggttgat
      181 ttataaccct tcgaattagg atcgtaataa atttccatgg ggaacgtttt ggtgaccatc
      241 agggccttga tgatctcgta ggcgatcttg ggcttgtcat ccttgctcgc cgttttgagg
      301 cgttcatgcc agtctttccc ctcgggagtt gccaggatgt ccgggttacc cgctaccagg
      361 tcgatgatga aacccatgtt gtccgtatgg tcggtgacgg cgagccagtc cagtggtctc
      421 gaaagcttca ccggtatgcc gtaagacgac acgacctctt cgcccctggc gaagcggtag
      481 gcatctgccg gaagcaggat ggcgccagcc gtgccggcgt cgaaggaaat cccggtatgc
      541 agatgcgaat cgccccacag cacttcgttc ggaaattcac gtttggcgta gggcgaatag
      601 gattcgccct tgtacttctc cgacagcatt ttgttgacgt cgacgtcgag cggcacgtcc
      661 tgtgcctgta gcgggccgct cagcattgca accgccgcga tcaggatcgg ggtactgagt
      721 ttgatggttc tgctattcat aacctggaac ctgtttcgat attgttacga agggttgtac
      781 cagatcggcg acgtgtaggc gcgttcctgg ccgatgagcg tcgcgccttc cggcacctcg
      841 gcgccgaagc gaaccttgtc gtacagcacc cagcgcgggg tcgggatctc gatcacgcgc
      901 gcgtagtaga acgcgctctg tttcgggtcg aactcggggt cgctccagac cgcggcaagc
      961 tccgacgcgc cgatggtgtt ggtccagttg gcggcctcga ggtcgaccgt gttgccgacc
     1021 agctcgcggg tgcggccgtc ttcgtcgatc ttgcggccgt cggagacggc aacgtcgtag
     1081 atgcgctcgt gggtcttgcc gtcctggtcg agccagccct tgacgatctg aatgcggtcg
     1141 aggttggcgc cgaccgggtc gcgcagcgcg tagaccatga aggtcggcgc ggtcttgccc
     1201 tcgctggccg gcggcaggtc gccgcccatc ggtacgcctt tttcgtaacc gatgaacgcg
     1261 ggctggcggc tgcgcaggtc gttgtcagtg tagtcccagc cgccgaagaa acgcaccggg
     1321 atacgcgggc cggtggtggc gtaggtttcc ttgcgcagca tcgcgtcgaa cagcgcggcg
     1381 cgcgtgttgt cggtggccca gacgccctgg tagccggatg cgaccgacgg gtcgcccgaa
     1441 atctcgccaa gcttcgactt gatgaacgga tgcgcgacac ggtctttcga cggttccacg
     1501 ctgaccgact tgccgaaaaa gttttcttcc tcggcggtgg cgagcccggt atggctgtcg
     1561 gtagcgccga ccatgccgaa cttgtacggg ttggtgccga gcttggcctc gagcatcagg
     1621 ccgcgcttga gcgcctcgcg cgcgtactcg ccggcgagca tctcgtcttt cttgagctcg
     1681 gtgatatcga ggttacccac gtcccagttt tcataatcgg cgaactcgtc gtccggcgac
     1741 aggaacgggt gggcttcgcc gtcgcccttg atctgcgtca tttcgtacag cggctcccag
     1801 cgcgcacgct gctcgacgta gttctggtcg accttgttgc cgtggtaagt cttgtcgacc
     1861 gggaacatcc agccgttcga caggttgccg ttgtgcgaca gcgcgaatgc ctggccgttg
     1921 gtctttttct ggtagttgtc gagccaggcg tacaggtcca tcggatcggt ggtgccgagc
     1981 ggcggctgcg tggtcagcgg gatgacctgc ctggcgcgca cgccaccgtc gcgcaggatg
     2041 acgttgcggt gcaggttgaa acccttgggc accgaagtcc actcgtagcc gatgaaggcg
     2101 gtgaagcgac cgggttcgtt gtaggtttcc gcggcgtcga cgat
//
LOCUS       DATCZV010000560         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_415892, whole genome shotgun sequence.
ACCESSION   DATCZV010000560 DATCZV010000000
VERSION     DATCZV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415892"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VIW27_12690"
     CDS             <1..991
                     /locus_tag="VIW27_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5740577.1"
                     /translation="VMSAQATVIPSGHEFFVEGAESLLEAGLRGGLALNYGCSNGNCG
                     LCKMRVVSGEVRRIGHHDYTLSAAEKGLGYMLGCCNTAVTDVVLEADEARGSSDIPKQ
                     RIPLRIKKIERPADDVLILGTRTPRTQRLRFLAGQAASLSIDGVGRGFYPIASCPCDD
                     MNLQFHIARNPRDAVATHLDTAAKINEQIDLEGPEGSFVLDENSPRALVFIAEGIGFA
                     SIKGLIEHAMALDAAERVYLFWVSSESLPYLHNLCRAWNDALDNFHYMALRSGEGITA
                     ELAAALESQQPAGLDYYACGGSALCAAVEGFIDERGVPRSQFSYHTIQQAVES"
     gene            994..1656
                     /locus_tag="VIW27_12695"
     CDS             994..1656
                     /locus_tag="VIW27_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5740578.1"
                     /translation="MSLKVLLIDDHMLFREGLISLLQRRNIEVLAAVGDGNEGIKLAQ
                     ELKPDIVLLDKRMPIIDGISVLKHLKKLDLQMPIVMLTTSSNEQDLLDALKAGASGYL
                     LKDMEPDGLVSALRDIHAGKTVVAPPLTSVLVRFVKGDMTISQSKGPFSELTPRESEI
                     LELMAEGQGNKLIARNLGISDGTVKLHVKAVLRKLGVHSRVEAAVMYIEHNHRAAEPA
                     DE"
     gene            complement(1706..>2009)
                     /locus_tag="VIW27_12700"
     CDS             complement(1706..>2009)
                     /locus_tag="VIW27_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5740579.1"
                     /translation="NPDPYYQQGGDMVRLGGMDYVLDPTGDAGNRISNMQLDDGTPIE
                     ASKTYKVAGWATVGSVSPGEPIWDVVANYLRRVKTARIEKLNTPKLKNVDNNPGIA"
BASE COUNT          386 a          640 c          615 g          368 t
ORIGIN      
        1 cgttatgtcc gcacaggcga ccgtgattcc gagcggtcac gaatttttcg tcgagggcgc
       61 cgagtcgctg ctcgaagccg gcctgcgcgg cgggctggcg ctgaactacg gctgcagcaa
      121 cggcaattgc gggctgtgca agatgcgcgt ggtgtccggc gaagtgcgcc gcatcggcca
      181 tcacgactac acgctgtccg cggccgagaa gggactcggc tacatgctcg gctgctgcaa
      241 taccgcggtc accgatgtcg tcctcgaagc cgacgaagca cgcggcagca gcgatatacc
      301 gaaacagcgc ataccgcttc gcatcaaaaa aatcgagcgc cctgccgacg acgtactgat
      361 cctgggtacc cgcaccccgc gtacccaacg cctgcgcttc ctcgccgggc aagcggcttc
      421 gctgtctatc gacggggtcg gccgcggttt ttacccgatc gccagctgtc cctgcgacga
      481 catgaacctg cagtttcata tcgcccgtaa tccgcgggac gcggtcgcca cccacctgga
      541 cacggcggcg aaaatcaacg agcagatcga cctggagggc cccgagggca gcttcgtgct
      601 cgacgaaaac tcgccccgcg cgctggtttt cattgccgag ggtatcggct tcgcatcgat
      661 caagggcctg atcgagcatg ccatggctct cgacgcggcg gaacgagttt atctattctg
      721 ggtcagcagc gagtcactgc cctacctgca taacctgtgc cgtgcctgga acgatgcact
      781 cgacaacttt cactacatgg cattgcgctc cggcgaaggc atcaccgccg agctcgcggc
      841 agccctcgaa tcgcagcagc ccgccgggct cgattactat gcctgcggcg gctctgccct
      901 gtgcgcagcg gtcgaaggat ttatcgacga gcggggcgtt ccccgctcgc agttttcgta
      961 tcataccatt cagcaagcgg tggaatccta aacatgagtt tgaaagtact gctaatcgac
     1021 gatcacatgc tgtttcgcga gggcctgatc agcctgttgc agcggcgtaa catcgaggtg
     1081 ctggccgccg tcggtgacgg taacgaaggc atcaagctgg cacaggaact gaaaccggac
     1141 atcgtcttgc tcgacaagcg catgccgatt atcgacggca tcagcgtact caagcacctg
     1201 aaaaaactcg atttgcagat gccgatcgtg atgctgacga ccagctcgaa cgaacaggat
     1261 ctgctcgacg cgctgaaggc cggcgccagc ggctacctgc tgaaggacat ggaacccgac
     1321 gggctggtca gcgcgctcag ggatatccac gccggcaaga ccgtggtcgc gccgcccctg
     1381 acctcggtac tggtgcgctt cgtcaagggc gacatgacga tttcgcagag caagggcccg
     1441 ttcagcgaac tgacgccgcg cgaaagcgag attctcgagc tgatggccga gggacagggc
     1501 aacaagctga tcgcgcgcaa tctcggcatc tccgacggca cggtcaagct gcacgtcaag
     1561 gccgtgctgc gcaaactcgg cgtgcattca cgcgtcgagg cggcggtcat gtacatcgag
     1621 cacaaccatc gcgccgcgga gcctgccgac gagtagcgcg gcaacggccg gccgccccgt
     1681 agccgggcct gtcgaacctg aagcgctagg cgattccggg attgttgtcg acgtttttca
     1741 gcttcggcgt gttgagcttt tcgatgcgcg cggttttgac ccgccgcagg tagttcgcga
     1801 ccacgtccca gatcggttcg cccggagaca ccgagcccac cgtggcccag ccggccacct
     1861 tgtaggtttt cgacgcctcg atcggcgtac cgtcgtcgag ttgcatgttc gatattcggt
     1921 tgccggcatc gccggtcgga tcgagcacgt agtccatgcc gccgagacgc accatgtcgc
     1981 cgccctgctg gtaatacgga tccgggttg
//
LOCUS       DATCZV010000561         3074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_680826, whole genome shotgun sequence.
ACCESSION   DATCZV010000561 DATCZV010000000
VERSION     DATCZV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680826"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VIW27_12705"
     CDS             <1..812
                     /locus_tag="VIW27_12705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5740580.1"
                     /translation="LLDAPQMQDICGSDYYRGGLETPGTAIIQPALFVRSPAAEALEN
                     QGERWRAITASGSVRAARVILAVNGLIETFGFFRRRLMHINLYASMTRALERREIDAL
                     GGSENWGFTPADPIGTTLRRIDGTGGTRLVIRNRCTYEASLRLPADRLAQVRRDHRRT
                     FQARFPMLAQLEMEYTWSGRLCLSRNEAWASGELAPGLFSACCQNGLGTTRGTIAGIA
                     AAELAAGGDQPSLVPGFPARQLPSRLFPEPFMTLGARGVIRFKEWRAGREL"
     gene            complement(1104..1457)
                     /locus_tag="VIW27_12710"
     CDS             complement(1104..1457)
                     /locus_tag="VIW27_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740581.1"
                     /translation="MVLEDKVINPALAQQLFRKSEMLKRKRAGEPAAVESFPRVVKAI
                     TSLWRHTESKKYLEELILVEGGKRRAGFPIEVQAELMFLYQLMLDQRRVLGLKGEKMT
                     VQRPPGTRFTMGHKG"
     gene            1727..2815
                     /gene="zapE"
                     /locus_tag="VIW27_12715"
     CDS             1727..2815
                     /gene="zapE"
                     /locus_tag="VIW27_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY5740582.1"
                     /translation="MKPSQRYRALIEQPGFVADAAQQRSADLLDALQARLLASAKTGW
                     WRGWRGKNRWPQARGLYFWGGVGRGKTLLMDLFFQSLPTEVPAERVHFHSFMNDVHRA
                     LQNHRSRREPLELVAAEIAHRSRVLCLDEFVIIDIGDAMIMAGLLQALLAQGVVLVTT
                     SNAAPRDLYRDGLQRARFLPAIDLIVDHCEIVNLDGDDDYRLRFLRQTELYAVPHGDD
                     VDAAIRARLERHLAPVQSEQQVLKINGRVMAPRYCAEDTVWFSFAELCETLRSQNDYL
                     ELARFFNTLILTGIRQMDGGDDDIARRFVLLIDVLYDHRVKLICSAAAMPDRLYTGKR
                     LEFEFERTASRLLEMQSAEYLGEAHALH"
BASE COUNT          555 a          949 c          980 g          590 t
ORIGIN      
        1 gcctgctcga cgcgccgcaa atgcaggata tttgcggcag cgactactat cgcggcggac
       61 tcgagacgcc cgggaccgcg atcattcaac cggcgctgtt cgtgcgctcg ccggccgccg
      121 aggctctcga aaaccaggga gagcgctggc gtgcgatcac cgccagcgga tcggtcagag
      181 ccgcgcgtgt gatcctcgcg gtcaacgggc tgatcgagac cttcggcttc tttcgtcgtc
      241 gactcatgca catcaatctc tatgcgtcga tgacgcgtgc cctcgagcgg cgcgagatcg
      301 acgcgctcgg cggcagcgag aactgggggt tcacccccgc cgacccgatc ggcacgacgc
      361 tgcggcgcat cgacggcacc ggcggcaccc gcctggtgat tcgtaatcgc tgtacctacg
      421 aggcgtcgct gcggctgccc gcggaccgcc tggcgcaggt gcggcgcgat caccggcgta
      481 ccttccaggc gcgcttcccg atgctggcgc agctggagat ggagtacacc tggtccggcc
      541 gtctgtgcct gagccgcaac gaagcctggg ccagcggcga actggcgccg ggactgttca
      601 gtgcctgctg ccagaatggt ctgggcacga ctcgtggcac gattgccggc atcgccgccg
      661 ccgagctggc cgcgggcggc gaccagccga gcctggtacc cggatttccg gcacggcagc
      721 tgccgtcgcg attgtttccg gagccattca tgaccctcgg cgcgcgcggt gtgattcgct
      781 tcaaggaatg gcgtgccgga cgcgagctct aggcgctcta gcgcccgcgc ccgcgggcat
      841 cgtgcaccat caggtcatgc ccgcgaaagc gggcatcttt caccttcggg gtcatgcccg
      901 cgaaagcggg catcccggga tggcggcgcg tcacgcagat tcccgcgtgc gcgggaatga
      961 cttcgggcag cgggaatgac tacgggcagc gggaatgact tccagtatcg tcatgcccgc
     1021 gaaagcgggc atcttgcacc ttggagtcat gcccgcgaaa gcgggcatct tgcgacgatc
     1081 gtgcgctcgg ccagggcctc gcgtcagccc ttgtggccca tcgtgaagcg ggttccaggc
     1141 ggccgctgaa cggtcatttt ctcacccttc aggccgagaa cgcggcgctg gtcgagcatc
     1201 aattgataaa gaaacatcaa ttcggcctga acctcgatcg gaaacccggc gcgacgtttg
     1261 ccgccctcga cgaggatcag ttcttcgaga tatttcttgc tctcggtgtg gcgccacagg
     1321 ctggttatcg ccttgaccac gcgtggaaac gattccacgg cggcgggctc gccggcccgc
     1381 ttgcgtttca gcatttcact cttgcgaaac agctgctgcg ccagcgccgg gttgattacc
     1441 ttgtcttcta acaccatgaa taccgactcg atattgccgg aacgacagcc ttgcggccgc
     1501 tttagtcccc gtattttatt ctttggccgc tcgcgcgact tgaaaagtat atcgtgaatg
     1561 gctttaaatt caacaaccgt gccgcagatc cgcagcaagg tactaaaaaa cattaaaaaa
     1621 acacgctaag atattaaaaa ataagattat ttatcggata ttcgctgtta aactcaggct
     1681 cgaatgcggg ccgatcgacg ggccgcgccg gggtatacag agttccatga aaccatcgca
     1741 gagataccgt gcattgatcg agcaacccgg ctttgtcgcc gacgcggcgc aacagcgctc
     1801 ggctgacttg ctggacgcat tgcaggcgcg cctgctggcc tcggcgaaga ccggctggtg
     1861 gcgcggctgg cgcggcaaaa accgctggcc gcaggctcgc ggtctgtatt tctggggcgg
     1921 ggtggggcgt ggcaagacac tgctgatgga cctgtttttt cagtccctgc ccaccgaggt
     1981 gccggccgag cgtgtgcatt ttcacagttt catgaacgac gtacaccgcg cgctgcaaaa
     2041 ccatcgttcc cggcgcgagc cactggaatt ggtggcggcg gagatcgcgc accgctcgcg
     2101 ggtgctgtgc ctcgacgaat tcgtgatcat cgatatcggc gacgcgatga tcatggcggg
     2161 cttgctgcaa gcgctgttgg cacagggcgt ggtgctggtg acgacgtcga acgcggcgcc
     2221 gcgggacctt tatcgcgacg gcctgcagcg cgcgcgtttc ctgccggcca tcgacctgat
     2281 cgtcgatcat tgcgagatcg tcaatctcga tggtgacgac gattatcgcc tgcgtttcct
     2341 gcgtcagacg gagctttacg cggtaccgca cggcgacgat gtcgacgcgg cgatccgtgc
     2401 ccgcctggaa cggcacctgg cgccggtgca gtccgagcag caggtactga agatcaacgg
     2461 gcgggtgatg gcgccgcgtt attgcgccga ggacaccgtc tggttcagtt tcgctgagct
     2521 ttgcgaaacc ctgcgcagcc agaatgacta cctcgagctg gcgcgctttt tcaataccct
     2581 gatcctgacc ggaattcggc agatggatgg cggcgacgac gatattgcac ggcgtttcgt
     2641 gctgctgatc gacgtgcttt acgatcaccg tgtcaagctg atttgcagtg ccgcggcgat
     2701 gcccgatcgg ctctataccg gcaagcggct agaattcgag ttcgagcgca ccgcgagccg
     2761 cctgctcgaa atgcaatcgg ccgaatacct aggcgaggca cacgccctgc actgagcgct
     2821 gcggatgggt atattgcatc ggttgaaccg cgccggcccg cggaccgctg aagctcgagt
     2881 gaaatagtgg gcgttcggat tcacaccggg actgcgatcg gtgttcggct caaaccatga
     2941 ttcggcacgc agacgcattc atgccgagcc agcccggaac agcgttgcga gatgcgggct
     3001 agtcggcagg cagggcctcg cgcacgcgct ttgcccagga ttcgaacagt tgcggactgc
     3061 tggccgcgac tacc
//
LOCUS       DATCZV010000562         5749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_468671, whole genome shotgun sequence.
ACCESSION   DATCZV010000562 DATCZV010000000
VERSION     DATCZV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468671"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VIW27_12720"
     CDS             complement(<1..665)
                     /locus_tag="VIW27_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5740583.1"
                     /translation="MDWSLLITQLLNGLQLGLLLFLLASGLTLIFGIMDFINLSHGSF
                     YMIGAYFCGTVVAITGSFFAGVVFGLLGMLIIGAVVEWFIARRLYRADHLDHVLVTFG
                     LILIFDTLVHLLWGAEGMAIPLPDILNGQIEIGTLVLPTYRVLIICCGLGVAALLYVL
                     VVRTRLGMLIRAGASNRTMVEALGVNINKLYLVVFALGAAMAGFAGMLIAPITEASIG
                     MGN"
     gene            complement(738..1925)
                     /locus_tag="VIW27_12725"
     CDS             complement(738..1925)
                     /locus_tag="VIW27_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740584.1"
                     /translation="MIIKAIRTLVVASAIIVSGAAQADSVKIGFVTTLTTPAAVIGED
                     MKNSVELALEHIGNKMGPLDVELIMEDDGFKPDIGKQKTDKLVKQDDVDFVAGYIWSH
                     VLLASAKSVLDTDKFLISANAGPSQIAGKRCHKNFFSTSWQNDQTPMAMGEHLNQKGV
                     KSLYVMAPNYAAGKDMVNGVERVFKGEILGKDMTKWGADAQLDFSAELAKAKASGAEA
                     LFVFYPGKAGGAFIKQYQQAGLQGNMDLYTVFTVDSIALPKLQAAGLSGVLGSKNTQE
                     WSPDILNAANQRFVGDYLKKHGRYPSFYGAQSYDAIMLINSAVQKVNGNLDDKDALRA
                     ALKAADFDSVRGKYSYGNNHMPIQNFYLREVVEDSEGRWTTKIVSTVFTNHQDIYAKD
                     CRM"
     gene            complement(2105..2947)
                     /locus_tag="VIW27_12730"
     CDS             complement(2105..2947)
                     /locus_tag="VIW27_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5740585.1"
                     /translation="MTATVEFVDLDWDGRAIRIEYCWLRTAAAERPLMVFLHEGLGSL
                     AMWKDFPAELCRRLDCRGLVYSRPGYGQSTPRPPAERWPVDFMHRQARQVLPLLLSAL
                     RIDTVAQPPWLLGHSDGASIALLYAAGFPERVAGVVALAPHIRVEELSVASIERARAA
                     YLETDLRDRLARYHADPDSAFWGWNDIWLDPAFRDWSIDSELAAIRCPLLAVQGVDDE
                     YGTLEQVHGIARRVDHARVVELAACGHSPQRDQPHELVELVAGFFATYTSADCRHGRA
                     GGEG"
     gene            complement(2944..4509)
                     /locus_tag="VIW27_12735"
     CDS             complement(2944..4509)
                     /locus_tag="VIW27_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY5740586.1"
                     /translation="MNTDAVAAPPAVFNYAAYLRDLNRERGDKTAYIDDTGSLAYGEL
                     FARVARFAGALLDAGIRREERVLLLMHDCNDWPVAFLGAIHAGIVPVAVNTLLPVGDY
                     LYMLQHSRAQAALVSNALLPTLREALASGDHQVGTVIVSRAEGGLQDGEQSFDAVLEA
                     AQTLAEPAATAADETCFWLYSSGSTGKPKGTVHSHANPYWTSELYGKGVLGITESDVC
                     FSAAKLFFAYGLGNALTFPLSVGASSVLLAGRPTPDVIYRRMVEYQPTLFFGAPTGYV
                     AMLAAADKPNPEELSLRLAVSAGEALPADLARRFSEYFGIDVIDGIGSTEMLHIFISN
                     IPGRVRFNSSGYPVPGYDIELRGDDDSPVAVGEIGDLYVRGPSAAIMYWNNRAKTRDT
                     FRGEWTKCGDKYIHNDDGSYTCSGRSDDMLRVSGQYVSPFEVEAALAEHDAVLEAAVI
                     GVADADGLTKSKAFVVLAEGGQTDAAELQEFVKSRLAPFKYPRFIEFVDELPKTATGK
                     IQRFRLREREAGA"
     gene            complement(4567..5028)
                     /locus_tag="VIW27_12740"
     CDS             complement(4567..5028)
                     /locus_tag="VIW27_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4863 family protein"
                     /protein_id="HEY5740587.1"
                     /translation="MSESEFRQQMVELTARIAGKPLDADLQDWLNRELGADSEEFQRL
                     QDSCRQGVAEGWLCQREAGGIRYGRVYRPDDELHGFSVDVVDMDDCVGPHHRHPQGEI
                     DLVMPLEDGALFDDQPAGWVVYAAGTAHHPTVSNGRALVLYLLPGGSIEFT"
     gene            5173..>5749
                     /locus_tag="VIW27_12745"
     CDS             5173..>5749
                     /locus_tag="VIW27_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY5740588.1"
                     /translation="MTASSGGPHRERGASPLLAALGERVRGLRERRGITRKSLAAATG
                     VSERHLANLEYGDGNPSVLVLQQVADALHCRAAELLDEPTTQSPEWLLLRSLLRPRDE
                     ATLRRIRHSIVELLGELPAAGGARRIALIGLRGAGKSTLGAMLARELGFEFVELSREI
                     ETLAGCTMGEIRALYGANAYRRHQRRALQSVI"
BASE COUNT         1037 a         1890 c         1804 g         1018 t
ORIGIN      
        1 tcgttaccca tgccgatgct ggcctcggtg atcggcgcga tcagcatgcc ggcgaaaccg
       61 gccatcgccg cgccgagcgc gaacaccacc aggtagagct tgttgatatt gacgccgagc
      121 gcctcgacca tggtgcggtt cgacgcgccg gcgcggatca gcatgccgag ccgcgtgcgc
      181 accaccagca cgtagagcag cgcggcgact cccaggccgc agcagatgat cagtacccgg
      241 taggtcggca gtaccagggt gccgatctcg atctgaccgt tgaggatatc gggcagcggt
      301 atcgccatgc cctcggcgcc ccacagcagg tgcacgaggg tgtcgaagat caggatcagg
      361 ccgaaggtca ccagcacgtg atcgaggtga tcggcacggt agaggcggcg cgcgatgaac
      421 cattcgacca cggcgccgat gatcaacata cccagcaggc cgaacaccac accggcgaaa
      481 aaggatcccg ttatcgccac caccgtaccg caaaaatagg cgccgatcat gtagaaggag
      541 ccgtgcgaca ggttgatgaa atccatgatg ccgaagatca gcgtcagccc cgaggcgagc
      601 aggaacagca gcagcccgag ctgcagcccg ttcaacaatt gcgtgatcaa taagctccag
      661 tccatgcgcg gcggccgggg tcgaaatcga gggaaagcgg cgcgcccggc ccgtgctgcg
      721 gccaggcgcg cgtcaactta catccggcaa tccttggcgt agatatcctg gtggttggta
      781 aacaccgtgg acacgatctt ggtggtccag cggccctcgc tgtcctcgac cacttcgcgc
      841 aggtagaagt tctggatcgg catgtggttg ttgccgtaag agtacttgcc gcgtaccgaa
      901 tcgaaatcgg ccgccttcag cgccgcgcgc agcgcgtcct tgtcgtcgag gttaccgttg
      961 actttctgca ccgcgctgtt gatcagcatg atcgcgtcgt aggattgcgc gccgtagaac
     1021 gacgggtacc tgccgtgctt cttcaggtaa tcgccgacga aacgctggtt ggcggcattg
     1081 agaatgtcgg gtgaccactc ctgggtattc ttcgaaccga gtaccccgga cagccccgcc
     1141 gcctgcagct tgggcagcgc gatcgaatcc accgtgaaca cggtgtaaag gtccatgttg
     1201 ccctgcagac ccgcctgctg gtattgcttg atgaacgcgc cgccggcctt gcccgggtag
     1261 aaaacgaaca gcgcttcggc gcccgacgcc ttggccttgg cgagctccgc ggaaaaatcg
     1321 agctgcgcgt cggcgcccca cttggtcatg tccttgccga gaatctctcc cttgaagacg
     1381 cgctcgacgc cgttgaccat gtccttgccg gcggcgtagt tcggcgccat gacatacagc
     1441 gacttgacac ctttctggtt caggtgctca cccatcgcca tcggtgtctg gtcgttttgc
     1501 cacgaggtcg aaaagaagtt cttgtggcag cgcttgccgg cgatctgcga cgggcccgcg
     1561 ttggcgctga tcagaaactt gtcggtgtcg agcaccgact tcgccgaggc cagcagcacg
     1621 tgcgaccaga tgtaaccggc gacgaaatcg acatcgtcct gcttgaccag cttgtcggtc
     1681 ttctgcttgc cgatgtcggg cttgaagcca tcgtcttcca tgatcagttc gacgtcgagc
     1741 ggtcccatct tgttaccgat gtgctcgagc gcgagttcga ccgagttttt catgtcctcg
     1801 ccgatcaccg ccgccggcgt cgtcagcgtg gtgacgaagc cgatcttgac gctgtcggcc
     1861 tgggcggcac cgctgacgat gatcgcgctg gcgaccacca aggtgcgtat ggcttttatt
     1921 atcatcttca ctctcctctg gagtctcttc agttgtcggc ggttgggccg ccggatttat
     1981 tgtcggggcc gcccggcggc ccgctacgaa tcatttgcct gctgcccggc ctggtcgatt
     2041 tacacctgcc ggggctggca aaacggcagc atagcggtat cgcgcgcgct tgaccatacc
     2101 gcagtcagcc ttccccgcca gccctgccgt gccggcaatc ggcgctagta taagtggcga
     2161 aaaaaccggc caccagttcg accagttcgt gcggctgatc gcgttgcggc gaatggccgc
     2221 aggccgccag ctccacgacc cgcgcgtggt cgacgcggcg cgcgatacca tgcacctgtt
     2281 caagagtacc atattcgtcg tcgacaccct gtaccgcgag cagcgggcag cgaatcgccg
     2341 ccagttcgct gtcgatcgac cagtcgcgaa acgccgggtc cagccagata tcgttccagc
     2401 cccagaaggc cgagtccgga tcggcatggt agcgcgcgag ccggtcgcgc aaatcggttt
     2461 cgaggtaagc ggcgcgcgcg cgctcgatgc tggcaacgct aagctcctcg accctgatat
     2521 gcggtgccag tgcgaccacg ccggcgaccc gctcgggaaa tccggccgcg tacagcagcg
     2581 cgatcgacgc gccatcgctg tggcctaata accacggtgg ttgggccacg gtatcgatgc
     2641 gcaacgccga gagcagcagc ggcaatacct gccgtgcctg gcgatgcatg aaatcgaccg
     2701 gccagcgctc ggctggcggg cgcggcgtcg attgcccgta gccgggccgc gagtagacca
     2761 gcccgcgaca atccagcctg cggcaaagct cggcgggaaa atccttccac atcgccagcg
     2821 aaccgaggcc ctcgtgcagg aataccatca acggccgctc ggcggcggcg gttcgcagcc
     2881 agcaatactc gatgcgaatc gcgcggccgt cccagtcgag gtcgacgaac tcgaccgtcg
     2941 cggtcacgcg ccggcttccc gttcgcgtag ccgaaagcgc tgaatcttgc cggtcgcggt
     3001 ttttggcaac tcgtcgacga actcgatgaa gcgcgggtac ttgaacggcg ccagccggct
     3061 cttgacgaat tcctgcaatt ccgcggcgtc ggtttgcccg ccctcggcga gtaccacgaa
     3121 agccttgctc ttggtcagcc cgtcggcatc ggcgacgccg attaccgccg cctcgagcac
     3181 cgcgtcgtgc tcggctagcg cggcctcgac ctcgaacggc gacacgtact gcccgctgac
     3241 ccgcagcatg tcgtcgctgc gtcccgagca ggtataactg ccgtcgtcgt tgtggatgta
     3301 cttgtcgcca catttggtcc actcgccgcg aaacgtgtcg cgcgtcttgg cgcgattgtt
     3361 ccagtacatg atcgccgcgc tcggaccgcg cacgtagagg tcgccgatct cgccgaccgc
     3421 gactggcgaa tcgtcatcgc cacgcaactc gatatcgtag cccggcaccg ggtaaccgct
     3481 ggaattgaag cgcacccggc ccggaatgtt ggaaatgaag atatgcagca tctcggtgga
     3541 accgataccg tcgatcacgt cgataccgaa gtactcgctg aagcgccgcg cgagatcggc
     3601 cggcagggct tcgccggcgg aaaccgcgag ccgcagtgac aactcctcgg gattcggctt
     3661 gtcagcggca gcgagcatcg ccacgtaacc ggtcggcgca ccgaagaaca gcgtcggctg
     3721 gtactcgacc atgcgccggt aaatgacgtc gggcgtcggc cgtccggcga gcagcacgct
     3781 gcttgcaccg acgctgagcg gaaaggtcag cgcattgccg aggccatagg cgaagaacag
     3841 cttggccgcc gaaaaacaga catcgctctc ggtgatgccg agcacgccct tgccatagag
     3901 ttcgctggtc cagtaagggt tcgcatggct gtgcaccgta cccttgggtt taccggtcga
     3961 tcccgacgag tacagccaga aacaggtttc gtccgcggcg gtggcggccg gctcggcgag
     4021 cgtctgcgcc gcttccagca cggcatcgaa ggactgttcg ccgtcctgca gaccgccttc
     4081 ggcgcgcgag acgattaccg tgccgacctg gtgatcgccg ctggccagcg cctcgcgcag
     4141 cgtcggcagc agcgcattcg acaccagtgc cgcctgcgcg cggctgtgtt gcagcatgta
     4201 caggtaatcg ccgaccggca gcagcgtatt taccgcgacc ggcacgatgc cggcatggat
     4261 cgcgccgaga aacgccaccg gccagtcgtt gcagtcgtgc atcaacaaca gcacccgctc
     4321 ctcgcgccga atcccggcat cgagcagcgc gccggcgaag cgcgcgacgc gcgcgaacag
     4381 ctcgccgtaa gcgaggctgc cggtgtcgtc gatataggcc gtcttgtcgc cgcgctcgcg
     4441 gttcaggtcg cgcaggtaag cggcgtaatt gaacacggct ggcggtgccg ctactgcgtc
     4501 ggtattcatc gattcctcct cggtttcggg cgcccgcgct tatagttgtc gggcttcgcg
     4561 ggccgctcag gtgaattcta tactgccacc gggcaataaa tacaggacca gcgcacggcc
     4621 gttgctgacc gtcggatggt gcgcggtccc ggccgcatag acgacccagc cggccggctg
     4681 atcgtcgaac agcgcgccgt cctcgagcgg catcaccagg tcgatctcac cctgcggatg
     4741 acggtggtgc gggccgacgc agtcgtccat atcgaccacg tcgaccgaga agccatgcag
     4801 ttcgtcgtcg ggccggtaaa cgcggccgta gcgaataccg ccggcctcgc gctggcacag
     4861 ccagccctcg gcgaccccct gccggcaact gtcctgcagg cgctggaatt cctcgctgtc
     4921 cgcacccagc tcgcggttca gccagtcctg caggtcggcg tcgagcggct tgccggcgat
     4981 gcgcgcggtc agctcgacca tttgctggcg aaattcgctt tcactcatct acttctcctt
     5041 catcgataac ccagagctgc cgccgaactt gcgcaagacc gcaaactgaa ttatggtgca
     5101 gcgacctgaa taaattagca ttatattgca tgcaacggtg attgctaagt ggccagttta
     5161 accaaaaacg cggtgaccgc gtcaagcggc ggcccgcatc gcgagcgcgg cgccagcccg
     5221 ttgctcgccg cactcggtga gcgcgtgcgc ggcttgcgcg aaaggcgcgg catcacccgc
     5281 aaatcgctgg ccgccgccac cggcgtctcg gaacgccacc tggccaacct cgagtacggc
     5341 gacggcaacc cgtcggtgct ggtgctgcaa caggtggccg atgcgctgca ctgccgggcg
     5401 gcggaactgc tcgacgagcc gaccacgcag tcgcccgaat ggctgctgtt gcgctcgctg
     5461 ctgcgccccc gtgacgaggc cacgctgcga cgaatccgtc actcgatcgt cgaattgctc
     5521 ggtgagctgc cggctgccgg cggtgcgcgg cgtattgcgc tgatcggcct gcgcggtgcg
     5581 ggcaaatcca ccctcggggc gatgctggcg cgcgaactcg gtttcgagtt cgtcgagctg
     5641 agccgtgaaa tcgagaccct cgccggctgc acgatgggcg aaatccgggc gctttacggt
     5701 gccaacgcct accgccggca ccaacgacgc gcgctgcaaa gcgtcatcg
//
LOCUS       DATCZV010000563         6523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_204708, whole genome shotgun sequence.
ACCESSION   DATCZV010000563 DATCZV010000000
VERSION     DATCZV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204708"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1217
                     /locus_tag="VIW27_12750"
     CDS             228..1217
                     /locus_tag="VIW27_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEY5740589.1"
                     /translation="MSPTSYQTSPFLSSVFDFMSVRRYSRRTIKSYLYWIKYFIVFHE
                     KRHPETMGPLEVEEFLTYLAVSRRVSVSTQKIALNALAFLYNKVLKQPLGDLGEFNRA
                     KTPGKLPVVLTRYEVRRLLDNMTGTSQLVASILYGSGLRRNEAVRLRVKDVDFDHHQL
                     QIWNGKGHYHRLTTLAPELVPRLRHQVEQVRLYLQQDSRLDRYAGVWMPDALSRKYSQ
                     ASRSLGWQYLFPSPRLAVDPQSGLLRRHHIHETNINRALKRATGLSGIEKQVTSHTLR
                     HSFATHLLESGADIRTVQEQLGHQDVKTTEIYTHVLKRGARGVRSPLSDLNNT"
     gene            1271..1936
                     /locus_tag="VIW27_12755"
     CDS             1271..1936
                     /locus_tag="VIW27_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5740590.1"
                     /translation="MAEDPLVIETGSAPDACVIWLHGLGADGHDFEPIVPELRLPAGL
                     AIRFIFPHAPMMPVTINQGFVMRAWYDIRSADIGAETDADGIRASAASVGAMVDAEIE
                     RGFAAERIVLAGFSQGGAIALQAGLRYPQRLAGILALSTYLPLADSLAAERSETNADV
                     PILMAHGSADPVIPLPLAVASRARLDALGYDVEWHEYPGLGHGVSPQEIAHIAAWIEG
                     VLK"
     gene            2159..2542
                     /locus_tag="VIW27_12760"
     CDS             2159..2542
                     /locus_tag="VIW27_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740591.1"
                     /translation="MEETQAFFDRYGPLLLARLDADNSLDNARSGPLDFIEEVLDSWS
                     QFARGRVLEAPSPQERAFWFALYQFEELVENPVTARLDPYEAILMQNLAEAREALRAG
                     RGLPDGLFATRPGELPEGFDDEELL"
     gene            complement(3039..4427)
                     /gene="argH"
                     /locus_tag="VIW27_12765"
     CDS             complement(3039..4427)
                     /gene="argH"
                     /locus_tag="VIW27_12765"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY5740592.1"
                     /translation="MSKKDSRMWGGRFQEPTDEFVQTFTASVAFDRRLAAADIEGSLA
                     HAKMLARVGVLDAEELQSIEDGLARIGAEIDAGEFEWSVALEDVHMNIEARLTQLIGD
                     AGKKLHTGRSRNDQVATDIRLYLRAQIAQARAGIRAFQSGLLDLAEREADAIMPGFTH
                     LQVAQPVSFGHHVLAWFEMLERDHSRLGDCAARLNQLPLGSAALAGTSYPLDREYTAE
                     LLGFDAPCRNSIDAVSDRDFAIEFCAAASICMMHLSRMAEELVLWSSAQFDFIRLPDR
                     YCTGSSIMPQKKNPDVAELVRGKSGRVSGHLVSLLMLMKSQPLAYNKDNQEDKEPLFD
                     TLDTLLGSLRAFADMVPALEVRRDRMYQAAEQGYATATDLADYLVRKGRAFRDAHEAV
                     GNAVAFAIKESKELGACSLDELRRFDAGIDEDVFEVLTLEGSLKARNHTGGTAPEQVL
                     RQVAAGRELIED"
     gene            4530..5588
                     /locus_tag="VIW27_12770"
     CDS             4530..5588
                     /locus_tag="VIW27_12770"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5740593.1"
                     /translation="MPDNSENSNIGIENTSYLPDFCDGDVFLRLLLVVELIAIVFALV
                     SFGGGSLFVHIALISLGMLWVGMTTAVLICLLMRRGWLGDHLRTTLLVFGIAMLMTLL
                     VSFVGLRLGTFIEPDTGPRGLVFTLTRNLSVAVILVGLALRYFYLHYESDMRLKVQAQ
                     ARLQALQARIRPHFLFNSMNTIASLTHDQPDLAEQAIENLSDLFRASLAAEPSISLQQ
                     ELELTHSYIDLEGLRLGERLRVDWRLPDEEPELKLPALTLQPLVENAIYHGIEPLAEG
                     GVIKIEIRDDGAAIGIAISNPRIAQRFSSRRDGNQMALDNIRERLTLAFGGAAAMTLN
                     EEDTRYTVELRIPKTRPE"
     gene            5585..6316
                     /locus_tag="VIW27_12775"
     CDS             5585..6316
                     /locus_tag="VIW27_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY5740594.1"
                     /translation="MKILIVDDEKPARDRLARMVSELPQHELIGQAVNGLEALGMTQA
                     MEPDIVLLDIRMPGMDGIEAARHLAKLDQPPAVIFTTAFSDHALEAFETHAVDYLLKP
                     VKQDRLQAAMDAAVRPTRAQAAKSGDLLSGLEPRQHICARVRGSLVLVPIEDIYYFHA
                     EQKYVTVRHTEGEVLIEDALKNLEDEFGDRFYRIHRNALVGLAWIAGMQSNDDGHRVT
                     FRDIDDTLEVSRRHLPGVRKIIKNL"
     gene            6317..>6523
                     /locus_tag="VIW27_12780"
     CDS             6317..>6523
                     /locus_tag="VIW27_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5740595.1"
                     /translation="MNASRTELRIATRSSQLALWQAKHVAACLESLYPQLKVSLLEMK
                     TRGDKFLDAPLAKVGGKGLFVKELE"
BASE COUNT         1187 a         1980 c         2064 g         1292 t
ORIGIN      
        1 taacagccta tttataagga cttcgtcctt ataataatta ttatcgaggt gactttataa
       61 ttttgggtgt attttttggg ggccatcatc ttattgattt ccctattgta aattcggtgt
      121 gtaaaagtct agcaggtatt atcgagactt tgtctcgata atacccttga ataaacgggt
      181 caaattgata tggttagccg gattagttta acgaaaggat tcgtgctatg tcgcccacct
      241 cgtatcaaac ctcgcctttt ttgagttccg ttttcgattt catgtcagtg cgccgttaca
      301 gccggcgtac gatcaagtcg tatttgtact ggatcaagta tttcattgtt tttcatgaaa
      361 aacggcatcc cgagacgatg gggccgctgg aagtcgaaga gtttttgacc tacctggcgg
      421 tatcgcgtcg agtatccgtt tcgacgcaaa aaattgcctt gaatgctttg gcctttttat
      481 acaacaaggt acttaaacag cccttgggtg acctgggtga gttcaatcga gcaaaaacgc
      541 cgggcaaact tcctgtggtt ttgacccgct acgaagtgag gcgattactg gacaacatga
      601 cagggacctc gcagttggtc gcttccatat tgtatggttc ggggctgcgg cgtaacgagg
      661 ctgtgcgctt gcgggtcaag gacgtcgatt ttgaccatca ccagctacag atctggaacg
      721 gcaagggtca ttatcaccgc ttgacgacgc tggcgccgga gctggtcccg cggttaaggc
      781 atcaggtcga gcaagttcgc ctgtacctgc agcaggatag ccggctggat cgatatgcgg
      841 gtgtctggat gccggatgcg ttgtctcgaa agtatagcca ggcgtcgcgc tcgctcggtt
      901 ggcaatacct gttcccgtcg ccgcggctgg ccgttgaccc gcaaagcggg ctactgcgcc
      961 ggcaccacat ccacgagact aacatcaacc gcgcgttgaa acgcgccacg ggcttgagtg
     1021 gcattgagaa gcaggtgacc agccatacgt tgcggcattc ctttgctacc catttgctcg
     1081 aatcgggtgc cgatattcgc acggtgcagg agcagctcgg tcaccaggat gtgaagacca
     1141 ccgaaatcta tacccatgtc ctgaagcgtg gggcccgtgg cgtgcgtagt ccgttgagcg
     1201 atttgaataa tacgtgatgc ctgctctcgc cgccgctata atgccggcct ttgagccaac
     1261 gagaatcgag atggcggaag atcccctggt catcgaaacc ggcagcgcgc ccgacgcctg
     1321 cgtcatctgg ctgcacgggc tcggcgccga cggccacgac ttcgagccca tcgtgcccga
     1381 gctgcgcctg cctgccgggc tggcgatccg tttcattttt ccgcacgcgc cgatgatgcc
     1441 ggtgactatc aaccagggtt tcgtgatgcg cgcctggtac gatattcgca gtgccgatat
     1501 cggcgccgag acggatgccg acggcatccg cgcctcggcg gcttcggtgg gcgcaatggt
     1561 cgatgccgag atcgaacgcg gcttcgccgc cgaacgcatc gtgctcgcgg gtttctcgca
     1621 gggcggcgcg attgcgttgc aggccggctt gcgttacccg caacgtctcg ccggtatcct
     1681 ggcgctgtcg acctacctgc cgctcgccga ttcgctggcc gcggagagga gcgagaccaa
     1741 cgccgatgtg ccgatcctga tggcgcacgg ctcggccgac ccggtgattc cgctgccgct
     1801 ggcggtcgcc tcgcgcgcgc ggctcgatgc actcggctac gacgtcgagt ggcacgagta
     1861 ccccggtctg gggcacggcg tttcgccgca ggagatcgcg cacatcgccg cctggatcga
     1921 gggcgttctg aagtagtgcc gcgccggcat acgccgcgcg gtcggcgatg cggcgcatcg
     1981 ggctcccgag atagcggtgt tagccggggg tcatgcccgc gcaggcgggc aatttgaaat
     2041 agcggcgtcc tgtcggattg tcgccgcgac gcggtcggcc cgcgtggaat gagtcgaagg
     2101 ttcgcaggcc tcgtgcgggt ggggcctgcg agtagaactg taaaatggag aaaacgtcat
     2161 ggaagaaacg caagcatttt ttgatcgcta tgggcccttg ttgctggccc ggctggatgc
     2221 agacaacagc ctggacaatg cgcgctccgg gccgctggat ttcattgagg aggtactcga
     2281 cagctggtcg cagttcgcgc ggggccgggt gctcgaagcg ccgagtcctc aggaacgggc
     2341 cttctggttt gcgctgtatc agttcgagga actggtcgag aatccggtaa ccgcacggct
     2401 cgatccctac gaggcgatac tcatgcagaa tctcgccgag gctcgcgagg cgctgagagc
     2461 ggggcgcggc ctgccggacg ggctgtttgc cacccggccc ggagaactcc cggaaggttt
     2521 cgacgacgag gagctgttat agcggcggca gcgttcggca gcgggtcacc acgcaagcga
     2581 gtcatcaccg cgcaagcggg gatcttgtaa tggtggcgct atccagcagt ccttccctgc
     2641 gcaagccgcc ccggccgggc agggatctag taacggccag ctgttatcag attcccgctt
     2701 tcgcggcaat gacacctcga ttcgtcattc gccgcgcagg cgagtcagcc ccgcgcaagc
     2761 cgcccgcggc ccgacggtga atccttcgat ggcggcatta tccgcaaggc attcgtcccg
     2821 caagcgcacg accgtgcggg cgatcttgcg agcggctatc gaaaaccggc gtcaccccgg
     2881 tccccgagcc ggggtccatc ggtgagattg ctttcgaatc acggagactg acgctttcag
     2941 cggaccgcac cgaataccgg gtcgagcccg gcatgacagc ttcgggttaa gtacaggggg
     3001 cgacgattat cgaaccgcgc cgggtcgaaa ccggccagct agtcctcgat cagctcgcgt
     3061 ccggcggcga cctgccgcag cacctgctcg ggggcggtgc cgccggtgtg gttgcgcgcc
     3121 ttgagcgagc cctccagcgt cagcacctcg aacacgtcct cgtcgatacc ggcgtcgaag
     3181 cgccgtaact catcgaggct gcaggcgccg agctccttgc tctccttgat cgcgaacgcg
     3241 accgcgttgc cgacggcttc atgggcgtcg cgaaatgcgc ggcccttgcg caccaggtag
     3301 tcggcgagat cggtggcggt cgcgtagccc tgttccgccg cctggtacat gcggtcgcgc
     3361 cgcacctcga gcgccggcac catgtcggcg aacgcgcgca gcgaaccgag cagcgtatcg
     3421 agggtgtcga acagcggttc cttgtcttcc tggttgtcct tgttgtaggc gagcggctgc
     3481 gatttcatca gcatcagcag gcttaccagg tggccgctaa cacgtcccga cttgccgcgt
     3541 accagttcgg cgacgtccgg gttcttcttt tgcggcatga tcgacgagcc ggtgcagtag
     3601 cggtccggca ggcgaatgaa gtcgaactgc gcgctcgacc acagcaccag ttcctcggcc
     3661 atccgcgaca ggtgcatcat gcagatcgac gccgcggcgc agaattcgat cgcgaagtcg
     3721 cggtccgaaa ccgcgtcgat cgagttgcgg cagggcgcgt cgaagccgag cagctcggcc
     3781 gtgtactccc gatccagcgg gtaggaggtg ccggccagcg cggcgctgcc gagtggcaac
     3841 tggttgaggc gcgcggcgca gtcgccgagg cggctgtggt cgcgttcgag catttcgaac
     3901 caggccagca cgtggtggcc gaagctgacc ggctgcgcca cctgcagatg cgtgaagccg
     3961 ggcatgatgg cgtcggcttc gcgctcggcg aggtcgagca gccccgactg gaaggcgcgt
     4021 atgccagcgc gcgcctgcgc gatctgcgcg cgcaagtaga ggcggatatc ggtcgccacc
     4081 tggtcattgc gcgagcgccc ggtgtgcagt ttcttgccgg cgtcgccgat cagctgggtg
     4141 aggcgcgcct cgatgttcat gtgcacgtcc tcgagtgcca ccgaccattc gaactcgccg
     4201 gcatcgattt ccgcgccgat ccgtgccagc ccgtcctcga tcgactgcaa ttcttcggcg
     4261 tcgagcacgc cgaccctggc gagcatcttc gcatgcgcca gcgaaccctc gatatcggcc
     4321 gcggcgaggc gccggtcgaa ggccaccgag gcggtgaatg tctgtacgaa ttcgtcggtg
     4381 ggttcctgga atcgcccacc ccacatgcgg gaatcttttt tgctcatttt tacaacgtcg
     4441 tgagtactga aaaacgcgcg cgagacggcg gcacatcgcg gcgaatattg ttgtatgctt
     4501 gcagtgcaag tccaacaaat caacacttta tgccagataa ttcggaaaat agtaacatcg
     4561 gcatcgaaaa cacgagttat cttcccgatt tctgcgacgg cgacgtcttc ctgcggctgc
     4621 tgctggtggt cgagttgatc gcgatcgtgt tcgcgctggt cagcttcggc ggcggcagcc
     4681 tgttcgtgca catcgcgctg atctcactgg gtatgctgtg ggtcggcatg accacggcgg
     4741 tgttgatctg cctgctgatg cgtcgcggct ggctcggcga tcacctgcgc accacgctgc
     4801 tggtgttcgg catcgccatg ctgatgacgc tgctggtcag tttcgtgggc ctcaggctcg
     4861 gtaccttcat cgagccggac accggcccgc gcgggcttgt cttcacgctg acccgcaacc
     4921 tgtcggtggc ggtcatcctg gtcggcctcg cgttgcgcta tttttacctg cactacgagt
     4981 cggacatgcg cctcaaggtg caggctcagg cgcggctgca ggcgctgcag gcgcgcatac
     5041 ggccgcactt cctgttcaac agcatgaaca ccatcgccag cctgacccac gatcagcccg
     5101 acctcgccga gcaggctatc gaaaacctgt ccgacctgtt ccgtgccagc ctcgcggccg
     5161 aaccgagcat ttcgctgcag caggaactcg aactgacaca cagctatatc gatctcgaag
     5221 ggctgcgcct cggtgagcgg ctgcgcgtcg actggcgcct gccggatgag gagccggagc
     5281 tcaagctgcc ggcgctgacc ctgcagccgc tggtcgaaaa cgcgatctac cacggcatcg
     5341 agccgctggc cgagggcggc gtgatcaaga tcgagattcg cgacgacggc gcggcgatcg
     5401 gtattgcgat ttccaatccg cgtatcgcgc agcgtttctc cagccgtcgt gacggcaacc
     5461 agatggcgct cgacaatatc cgcgagcgac tcacgctggc cttcggcggc gcggcggcga
     5521 tgactctcaa cgaggaagat acgcgctata ctgtagagtt gagaattccg aaaacgaggc
     5581 ccgagtgaag atacttatcg tcgacgatga gaaaccggcg cgcgatcgcc tcgcgcgcat
     5641 ggtcagcgaa ttgccgcagc acgagctcat cggccaggcc gtcaacgggc tcgaggcgct
     5701 cggcatgacg caggcgatgg agcctgacat cgtgctgctc gacatccgca tgcccggcat
     5761 ggacggcatc gaggccgcgc gccatctcgc caagctcgac cagccgccgg cggtgatctt
     5821 tactaccgcg ttttccgatc acgcgctgga agccttcgaa acccatgccg tggattacct
     5881 gctgaagccg gtcaagcagg atcgcctgca ggcggcgatg gacgccgcgg tgcggccgac
     5941 ccgcgcgcag gccgcgaaga gcggcgacct gctctccggc ctcgagccgc gccagcacat
     6001 ttgtgcgcgc gtgcgcggca gcctggtgct ggtgccgatc gaggatatct actattttca
     6061 cgccgagcag aaatacgtca ccgtgcgcca caccgagggc gaggtgctga tcgaggacgc
     6121 gctgaaaaat ctcgaggacg aattcggcga tcgtttctac cgcatccatc gcaatgcgct
     6181 ggtcgggctg gcgtggatcg ccggtatgca gtctaacgat gacggtcaca gggtgacctt
     6241 tcgcgatatc gatgatactc tcgaagtcag tcgccggcat ttgccgggtg tacgcaaaat
     6301 catcaagaat ctttgaatga acgcatcccg aaccgaactg cgcatcgcca cgcgcagtag
     6361 ccagcttgcg ctgtggcagg cgaagcacgt cgccgcctgc ctcgagtccc tgtatccgca
     6421 gctgaaagtg tcgctgctgg aaatgaaaac ccgcggcgac aagttcctcg acgcgccgct
     6481 cgccaaggtc ggcggcaagg ggctgttcgt caaggagctc gag
//
LOCUS       DATCZV010000564         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_734121, whole genome shotgun sequence.
ACCESSION   DATCZV010000564 DATCZV010000000
VERSION     DATCZV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734121"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VIW27_12785"
     CDS             complement(<1..197)
                     /locus_tag="VIW27_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011047741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY5740596.1"
                     /translation="MSTNSHRLRLAWYGATVFLSSAVLLVLEITAGRLIAPYVGVTIY
                     SWTSIIGVILAGLSLGNWIGGR"
     gene            complement(288..1421)
                     /locus_tag="VIW27_12790"
     CDS             complement(288..1421)
                     /locus_tag="VIW27_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEY5740597.1"
                     /translation="MEQGLLQLWASTGLYNFTVGQAIMILAGGVLIYLAIAKGFEPLL
                     LIPIGFGGILANIPIAEIAGPEGLLGVLYSAGIESGVFPLLIFMGVGAMTDFGPMLSR
                     PSLIILGAAAQFGIFVTLFGAIAMNLVPGIEFSLADAAAIAIIGGADGPTAIYVGSRL
                     APDLLGAIAVSAYSYMALVPIIQPPIMKLLTTEEERKTRMTQLRHVGKLEKIIFPLLL
                     LLITLLLLPAATPLVGMLCFGNLLKESGVVERLSNTAQNELINIVTIFLGLSVGSKLA
                     ADKFLNVETMGILLLGMFAFSIGTAAGVIMGKIMYRVTGGQVNPLIGSAGVSAVPMAA
                     RVSNKVGLQANPQNFLLMHAMGPNVAGVIGSAVAAGVLLAIVG"
     gene            complement(1768..>2578)
                     /locus_tag="VIW27_12795"
     CDS             complement(1768..>2578)
                     /locus_tag="VIW27_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5740598.1"
                     /translation="TPTSQIVGTQSVINVLGGERYKSISKETAGVLRGEYGATAAPVN
                     AELQERVLAGDQPVTCRPADLIEPEMDKLTGELREIAAAEGIELCDNEIDDVLIYALF
                     QQVGINFLQNRGNPDAFEPAPGSEPAAAPAAVPEAAAPQPAAATAGEVESYRVSVNGT
                     EYDVVVGPGDADISSITPAAQPASAASPPSAAQPPAAAGGTEIRAPLAGSVLDILVAV
                     GDQVNDGDPVMIVEAMKMETEIRASVSGKVQSIAIKKGDKIAADQSLMTVA"
BASE COUNT          493 a          818 c          818 g          449 t
ORIGIN      
        1 cggccgccga tccagttgcc gagcgacagg ccggcgagga tcacgccgat gatcgacgtc
       61 cagctgtaaa tggtgacgcc gacgtaaggc gcgatcagtc gtccggcagt gatttccagc
      121 accagtagca ccgccgagct caggaacacg gtcgctccgt accacgccag gcgcaatctg
      181 tgcgagttag tactcatccc cgccgcgccc tcgcggtaat gtgggtcaga tccaaattag
      241 attggcgcat ttaattcaga tctgacccaa attaatggaa gacggggcta gccgacgatg
      301 gcgagcagca cgccggcggc gaccgcggag ccgatcacgc cggcgacatt gggtcccatc
      361 gcgtgcatca gcaggaagtt ctgcggattg gcctgcagcc cgaccttgtt ggagacgcgc
      421 gccgccatcg gcaccgccga aaccccggcc gagccgatca gcgggttgac ctggccgccg
      481 gtgacgcggt acatgatctt gcccatgatc acgccggccg cggtgccgat cgaaaaagcg
      541 aacatgccga gcaacaggat acccatggtc tcgacgttca ggaacttgtc ggcggcgagc
      601 ttggaaccca ccgacagccc gaggaagatg gtcacgatat tgatcagttc gttctgcgcg
      661 gtattcgaca gccgctcgac cacgccgctt tccttgagca ggttgccgaa gcacagcatc
      721 cccaccagcg gcgtcgccgc cggcagcagc aacagcgtga tcagcagcag caacagcggg
      781 aaaatgattt tttcgagctt gccgacatgg cgcagctggg tcatgcgggt cttgcgctct
      841 tcctcggtag tcagcagttt catgatcggc ggctgaatga tcggcaccag cgccatgtag
      901 gaatacgcgg atacggcgat cgcgccgagc aggtcgggcg ccaggcgcga accgacgtag
      961 atcgcggtcg gcccgtcggc gccgccgata atcgcgatcg ccgcggcatc ggcgagcgag
     1021 aactcgattc ccggcaccag gttcatcgcg atcgcaccga acagggtgac gaagatgccg
     1081 aactgcgccg cggcgccgag aatgatcagg ctgggccgcg acagcatcgg tccgaagtcg
     1141 gtcatcgcgc cgacgcccat gaaaatcagc aacggaaaca ccccgctctc gatgccggcg
     1201 ctgtagagca ccccgagcaa accctcggga ccggcgatct cggctatcgg aatattggcg
     1261 agaataccgc cgaagccgat cgggatcagc aacagcggct cgaagccctt ggcgatcgcc
     1321 aggtaaatca gcaccccgcc ggccaggatc atgatcgcct ggccgacggt gaaattatac
     1381 aacccggtcg aagcccagag ctgcagcaga ccctgttcca tcgctcagcc tgcagcttgc
     1441 gccggcatcc cggatgctgg cgcaggacag atgcaactgg acgccggcct ggactgacaa
     1501 aatcgcccgg agtgattttg aacatcgacg catcgcgtcg atggccccgc gggggcggcg
     1561 ggcaggacgc ccgccgtaaa gccatagcca atgctatggc ttgaagaaag gccaaggcca
     1621 gttgtatctg gacaagccag agccgggata ggattcggac cgatttctac tactcttaaa
     1681 agactgatcc aggtaacttt caatttattc attggttata gccattggaa agaggtccga
     1741 attcggccgg cgtaagctgc aggctgccta agccactgtc atcagggatt gatcggcggc
     1801 gatcttgtcg cctttcttga tcgcgatcga ctgtaccttg ccgctgacgc tggcgcgaat
     1861 ttcggtctcc atcttcatcg cttcgacgat catcaccgga tcgccgtcgt tgacctggtc
     1921 gccgaccgca accaggatat cgagcacgct gccggcgagc ggcgcgcgga tttcggtgcc
     1981 gccggcggcc gccggcggtt gcgctgccga tggcggggaa gcggctgacg ccggttgcgc
     2041 ggccggggtg atgctgctga tatcggcgtc gccggggccg acgacgacat cgtactcggt
     2101 gccgttgacg ctgacacggt agctttcgac ttcgccggcg gtcgctgccg ccggctgcgg
     2161 cgccgctgcc tccggcactg ccgccggcgc ggcggcgggt tcgctgcccg gcgccggttc
     2221 gaaggcatcg ggattgccgc gattctgcag aaagttgatg ccgacctgct ggaagagagc
     2281 gtaaatcagc acgtcgtcga tctcgttgtc gcaaagctcg atgccctcgg cagccgcgat
     2341 ttcgcgcagc tcgccggtca gcttgtccat ctccggctcg atcaggtccg ccgggcggca
     2401 agtcaccggt tggtcgccgg ccagcacgcg ctcctgcaac tcggcattga ccggcgccgc
     2461 ggtggcgccg tactcaccgc gcagcacgcc ggcggtttcc ttgctgatcg atttgtaacg
     2521 ctcgccgccg agcacgttga tgaccgactg ggtgccgacg atctgcgagg tcggcgtc
//
LOCUS       DATCZV010000565         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_363211, whole genome shotgun sequence.
ACCESSION   DATCZV010000565 DATCZV010000000
VERSION     DATCZV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363211"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VIW27_12800"
     CDS             complement(<1..281)
                     /locus_tag="VIW27_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5740599.1"
                     /translation="MLRAANLADRAGARKWPAPVAWPGIQPIALRGQDSMLSTAKGSL
                     SLYMQIKDLLAARIGRGDWLPGSMIPSEVQLARELGVSQGTVRQAITELV"
     gene            393..674
                     /locus_tag="VIW27_12805"
     CDS             393..674
                     /locus_tag="VIW27_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY5740600.1"
                     /translation="MIDFVVHDEGDSVGVVVVEGVKAGDQLSGWIMDQDIEIKFEAAS
                     DIPIGHKLAIKPMKQGDTVIKYSTDIGKVVADIGKGEHAHVHNIKTKRW"
     gene            722..1897
                     /locus_tag="VIW27_12810"
     CDS             722..1897
                     /locus_tag="VIW27_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY5740601.1"
                     /translation="MAQDLSSATFSGYRRENGRVGVRNHVIILPVDDLSNAACEAVAN
                     NIPGTMALPHPYGRLQFGEDLELHFRTLIGTGANPNVAAVVVIGIESGWTQKIVDGIA
                     ATGKPVTGFGIEQHGDHETIMQASKVAKEYLQWASELVREPASVSELWVSTKCGESDT
                     TSGCGANPTVGNAFDKLYPHKATLLFGETSEITGGEHLVAARCRNDEVRERFMFMFDR
                     YQDLIDRFKTSDLSDSQPTKGNIEGGLTTIEEKALGNIQKIGKECVVDGVLDKAEAPT
                     GPGLWFMDSSSAAAEMVTLCAAAGYVVHFFPTGQGNIIGNPIIPVIKLCANPRTVRTM
                     SEHVDVDVSGLLRKEMTPGEAGDALLDMMFRTANGRLTAAESLGHREFVLTRLYESA"
     gene            1952..>3072
                     /locus_tag="VIW27_12815"
     CDS             1952..>3072
                     /locus_tag="VIW27_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5740602.1"
                     /translation="MRGADLLTQTLAKAGVKLIFSLSGNQIMPIYDACIDAGIRIIHT
                     RHEGAAVYMAEAYAQLTGEVAVAMVTAGPGFASGLGPLVTARASESPILLLSGDSPRS
                     GDGRGCFQELDQVGISKPLTKLSLRPRTAENLGSDTALALRVAASGRPGPVHLALPFD
                     LLNSKIGDLVLPAAQNFEPVVIAAGNQALATIIDTLAAAERPIVLTGPLLNPSRAPQI
                     RHLAQALDAPVVAMESPRGLKDPSLGNFAAALAQADVILSLGKIIDHTTGFGQPPAFG
                     SESKILVVDPDTGELERARRALRSRLVLAHRADADATARALISMAGATADRSAWRREV
                     ADAITYRFPEPKLTAGAAMHPAAVCAAVQRLLERADDPLL"
BASE COUNT          616 a          989 c          916 g          551 t
ORIGIN      
        1 accagctcgg tgatcgcctg ccgtaccgta ccctgactga cgccgagttc gcgggccagt
       61 tgcacctcgc tgggaatcat actgccaggc agccagtccc ccctgccgat tctcgccgcg
      121 agcagatcct tgatttgcat atacagcgac aacgagcctt ttgcggttga tagcattgag
      181 tcctgacctc gtaaggcaat cggttgaatc ccgggccagg caacgggcgc cggccatttg
      241 cgcgcccctg ccctgtcagc caggttagcg gctcgcaaca tgaatatacc agagatttac
      301 gttttagtct tatataagac tagttgacta cccgtatatt tccatgcaaa attcaaagga
      361 tctgttccgc acaatttacc ctggagaagc aaatgataga ttttgtcgtg cacgacgaag
      421 gagacagcgt cggcgttgtc gtggttgagg gcgtcaaggc cggcgaccag ctgagcggct
      481 ggataatgga ccaggacatc gagatcaagt tcgaggcggc cagcgacata cccatcggcc
      541 acaagcttgc gatcaagccc atgaaacagg gcgataccgt gatcaagtac agcacggaca
      601 tcggcaaggt cgttgcggat atcggcaagg gcgagcacgc ccacgtccac aatatcaaga
      661 ccaagcgctg gtaagtgacg ccagtcacca gcccaaccat cctgtttcgg agaattcacc
      721 aatggctcaa gatttaagtt cggcaacttt ttccggatac cggcgcgaaa acggtcgtgt
      781 cggggtccgc aaccatgtca tcatcctgcc ggtcgatgac ctgtccaacg cggcctgcga
      841 ggccgtggcc aataacattc cgggcaccat ggcgctgccc cacccctacg gcaggctgca
      901 gttcggtgaa gacctcgagc tgcatttccg taccctcatc ggtaccggcg ccaatcccaa
      961 cgtcgcagcc gtcgtcgtca tcggcatcga gagtggctgg acccagaaga tcgtcgacgg
     1021 catcgccgcc accggtaagc cggtcaccgg tttcggtatc gagcagcatg gcgaccatga
     1081 aaccatcatg caggcgtcaa aagtcgccaa ggaatacctg caatgggctt ctgaactggt
     1141 tcgcgagccg gcatcggtca gcgaattgtg ggtctccacc aagtgtggag agtccgatac
     1201 cacgtccggc tgcggcgcca acccgacggt cggcaatgcc ttcgacaagc tgtatccgca
     1261 caaggcgacg ctgttgttcg gcgaaacctc ggaaatcacc ggtggtgaac acctggtcgc
     1321 ggcccgttgt cgcaacgatg aagtgcgcga gcgcttcatg ttcatgttcg accgctacca
     1381 ggacctgatc gaccgcttca agacttccga cctgtccgat tcgcagccca ccaagggcaa
     1441 catcgagggc gggctgacca cgatcgagga aaaagcactc ggcaatattc aaaagatcgg
     1501 caaggaatgc gtcgtcgacg gcgtcctcga caaggccgaa gcgccgaccg ggcccggcct
     1561 ctggttcatg gactcgtcgt ccgcggcggc ggaaatggtt accctgtgcg cagcggcggg
     1621 ttacgtggtg cattttttcc ccaccggcca gggcaatatc atcggcaacc cgattattcc
     1681 ggtgatcaag ctgtgcgcca atccgcgcac cgtgcgtacc atgagcgagc acgtcgatgt
     1741 cgacgtctcc ggcctgctgc gcaaggaaat gacgcccggg gaagccggtg atgcactgct
     1801 ggacatgatg ttccgcaccg ccaatggtcg cctgaccgca gccgaatcac tcggacatcg
     1861 cgagttcgtg ctgacccgcc tgtacgaaag cgcgtaatcc cctcgaagca gcccctcctg
     1921 cacggagggg ctgccggatt cggattcgat catgagaggc gccgacctgc tgacgcaaac
     1981 cctggcaaag gccggggtca aactgatctt ttccctgtcc ggaaaccaga tcatgccgat
     2041 ctacgatgcc tgcatcgatg ccgggattcg tatcatccac acgcgtcacg agggcgccgc
     2101 ggtgtacatg gcggaagcct acgcccagct gaccggcgag gttgcggtag ccatggtgac
     2161 cgccggcccc ggtttcgcca gtggcctcgg gccgctggtc accgcgcggg cctcggaaag
     2221 cccgatactg ctgttgagcg gcgactcgcc gcgctccggg gatggcaggg gttgcttcca
     2281 ggagctcgac caggtcggca tcagcaagcc gctcaccaag ttgtcgctgc gcccgcgaac
     2341 tgccgaaaac ctgggctccg atacggcgct ggcactgcgc gtcgcggctt ccggcaggcc
     2401 cggcccggta catctggcct tgccgttcga cctgttgaac tccaagattg gcgacctggt
     2461 gctgccggcc gcgcagaatt tcgagcccgt ggtcatcgcc gcgggcaacc aggcgctggc
     2521 taccatcatc gataccctgg ccgccgccga acggccgatc gtattgaccg ggcccttgct
     2581 gaatccgagc cgtgcgccgc aaattcggca cctggcccag gcactcgacg ccccggtagt
     2641 cgcgatggaa agtccgcgtg ggctgaaaga tccgagcctc ggtaacttcg cagcggcgct
     2701 ggcgcaggcg gacgtcatcc tctcgctcgg caagataatc gaccacacca cgggattcgg
     2761 tcagccaccc gctttcggca gcgaaagcaa aatactggtg gtcgatcccg acactggcga
     2821 actggaacgc gcgcggcgag cactgcgctc gcgcctggtg ctggcgcatc gcgcagacgc
     2881 cgacgccacc gcacgggcat tgatctcgat ggctggcgcg accgccgacc gctccgcctg
     2941 gcggcgcgaa gtcgccgatg cgatcaccta tcgattcccg gagcccaagc ttaccgcagg
     3001 cgccgcgatg caccccgcgg cagtctgcgc tgccgtgcaa cggctgctgg agcgcgccga
     3061 cgatcccctg ct
//
LOCUS       DATCZV010000566         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_46477, whole genome shotgun sequence.
ACCESSION   DATCZV010000566 DATCZV010000000
VERSION     DATCZV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46477"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VIW27_12820"
     CDS             complement(<1..1344)
                     /locus_tag="VIW27_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5740603.1"
                     /translation="MNHATAADLSSTERWQSLDGAHHLHPFTNPDDLRKKPVRIIESA
                     EGVYLTDSDGNRLLDMMAGLWCVNMGYGRRELIEAAYAQIQQLPYYNAFFKTSTPPAA
                     ELSALLSEVTPAHINHAFFTSSGSEANDTVLRLVWRYWDLMGKPEKRIVISRKNAYHG
                     STIAGSSLGGMEEMHKQGGVIPDIVHVEQPNWFAKRCELGSDLDRDEFGRQAAQSIAD
                     KIEELGVERVAAFIAEPIQGAGGVIVPPDSYWPEVKKICDHYGILLVADEVICGFGRT
                     GEWFGSDYYQLKPDLMPIAKGLSSGYLPIGGVMVADRIMETLAAGGEFNHGYTYSGHP
                     VCAAVAIANIRLMQSENIVQTVRESTSPYLQQRFRELDDHPLVGEVRGLGMLGALELV
                     EDKQNYVRFDSKRGVGTICRDFCFDNHLVMRAVGDTMIISPPLILQHSDVDDFIER"
     gene            complement(1356..>2077)
                     /locus_tag="VIW27_12825"
     CDS             complement(1356..>2077)
                     /locus_tag="VIW27_12825"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5740604.1"
                     /translation="INLLHGEPLELADQAFLFKRTVREAALRHDFYATFMAKPMERQP
                     GSAMHIHQSVEDIESGENIFSTPEGKPNDLLLSHIAGLQRYLPEAMALMTPYVNSYRR
                     IVREMAAPINFHWGFDNRTTGFRIPDNNPHSMRIENRIASADVNPYLAIATSLACGFI
                     GMKQGLKPTEPLKGSAYAEPMQLSRHWYDSLRRLDDCDELRDILGEKFVDVYVSVKEA
                     EFDNFLNVISPWEREHLLLKV"
BASE COUNT          366 a          600 c          677 g          434 t
ORIGIN      
        1 gcgctcgatg aaatcgtcga catccgagtg ctgcagtatc agcggcggcg agatgatcat
       61 cgtgtcgccg accgcgcgca tcaccaggtg gttgtcgaag caaaagtcgc ggcagatcgt
      121 accgacgccc cgcttgctgt cgaacctgac gtagttttgc ttgtcctcga ccagttccag
      181 cgcgccgagc ataccgaggc cgcgtacctc gccgaccagc gggtgatcgt cgagttcgcg
      241 aaaacgctgc tgcaggtaag gcgaagtcga ttcgcgcacg gtttgtacga tgttttcgct
      301 ctgcatcagt cgaatgttgg cgatcgccac cgcggcgcag accgggtgac ccgaataggt
      361 ataaccgtgg ttgaattcgc cgccggcggc gagcgtctcc atgatacggt cggcaaccat
      421 gacgccgccg atcggcaggt agcccgacga caggcccttg gcgatcggca tcaggtcggg
      481 cttcaactgg tagtaatcgc tgccgaacca ttcgccggta cggccgaaac cgcagatgac
      541 ttcgtcggcg accagcagga tgccgtagtg atcgcagatc ttcttgactt cgggccagta
      601 actgtccggc ggtacgatca cgccaccggc accctggatc ggttcggcaa tgaacgcggc
      661 gacgcgctcg acgccgagtt cctcgatctt gtcggcgatc gattgcgcgg cctggcggcc
      721 gaattcgtcg cggtcgagat cgctgccgag ttcgcagcgt ttggcgaacc agttcggctg
      781 ttcgacgtgc acgatgtcgg gtatgacacc gccctgcttg tgcatctctt ccatgccgcc
      841 gaggctcgag ccggcgatgg tgctgccatg ataggcattc ttgcggctga tcacgatgcg
      901 cttctcgggc ttgcccatca ggtcccagta gcgccatacc aggcgcagta cggtatcgtt
      961 ggcttccgag ccggaactgg tgaaaaatgc gtgattgatg tgtgccggcg taacttcgga
     1021 cagcagcgcc gacaattccg ctgccggcgg cgtcgaggtc ttgaagaagg cgttgtagta
     1081 gggcagctgc tggatctgcg cgtaggcggc ctcgatcagc tcgcggcggc cgtagcccat
     1141 gttgacgcac cacaggccgg ccatcatatc gagcaggcgg ttgccatccg agtcggtcag
     1201 gtagacgccc tcggccgact cgatgatgcg caccggtttc ttgcgcaggt cgtcgggatt
     1261 ggtaaacggg tgcaggtgat gggcgccgtc gaggctttgc catctctcgg tactgctcag
     1321 atcggccgcg gttgcatggt tcatggtttt caccttcaga ctttcaacag tagatgctcc
     1381 cgttcccagg ggctgatgac gttgaggaaa ttatcgaact cggcttcctt gaccgagacg
     1441 tagacgtcga cgaatttctc gccgaggatg tcgcgcagtt cgtcgcagtc gtccaggcgg
     1501 cgtagcgaat cgtaccagtg gcgcgacagc tgcatcggtt cggcataggc gctgcccttg
     1561 agcggctcgg tcggcttcaa tccctgcttc atgccgatga acccgcaggc cagcgaagtg
     1621 gcgatggcga ggtagggatt gacgtcggcc gaggcgatgc gattttcgat gcgcatcgaa
     1681 tgcggattgt tgtcggggat gcgaaagccg gtggtgcggt tgtcgaagcc ccagtggaaa
     1741 ttgatcggcg ccgccatctc gcgcacgatg cggcggtagg aattcacgta gggtgtcatc
     1801 agcgccatcg cctccggcag gtagcgctgc agaccggcga tgtggctcaa cagcaggtcg
     1861 ttgggcttgc cctcgggagt cgaaaaaata ttctctccgg attcgatatc ctcgacgctc
     1921 tggtggatat gcattgcgct gcccggctgg cgttccatcg gcttggccat gaaagtggcg
     1981 tagaagtcgt ggcgcagcgc ggcctcgcgc accgtgcgct tgaacaggaa tgcctggtcg
     2041 gcgagttcca gcggttcgcc gtgcagcagg ttgatct
//
LOCUS       DATCZV010000567         5139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_363347, whole genome shotgun sequence.
ACCESSION   DATCZV010000567 DATCZV010000000
VERSION     DATCZV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363347"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VIW27_12830"
     CDS             <1..617
                     /locus_tag="VIW27_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740605.1"
                     /translation="PLNDASVEAVVAYQPDLVLASTFAAWDTARLLRRLGYEVRQIPP
                     VGSIADIYDLLRQVGDWTGNAARAASLAAEMRRRLADIERRYAARPEKTVIAYAPNGF
                     TVGGGTLENDVFEHAGYRNLAAEMGIEGFQPIALETLLAAEPDVLQIDRDLSRPDSLA
                     TAYVGHPVLERVSEQREQLDIPLRLRICGGPMIVEAVEAMARRR"
     gene            614..1618
                     /locus_tag="VIW27_12835"
     CDS             614..1618
                     /locus_tag="VIW27_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5740606.1"
                     /translation="MTALKPRPAVLYAVLLPLLLLALLLALQVGPVDISLAKAWRDWT
                     GGQTTIETLILFELRLPRALLALAIGATLGLAGASLQGLLRNPLAEPGIIGVSNGAAL
                     GAVIVFYYGLAGLAWFVLPLGGLLGAMISVAVIFMLIGRSRSIVTLILAGIAINAIGG
                     ALIALALNFADSPYAMQEIVFWLLGSVANRSVNELYIALPFMVAGWLLMLYCGRFLNA
                     LTLGEETAASMGFDVARMRLLLVIGVTASVGAAVSVAGNIGFVGLVVPHLLRPFVAFE
                     PRRLLPASMAAGAILLLVADIAVQLLSETREMKLGVITSLVGAPFFLFLILKSRNMLQ
                     "
     gene            1615..2403
                     /locus_tag="VIW27_12840"
     CDS             1615..2403
                     /locus_tag="VIW27_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740607.1"
                     /translation="MNLLEGDKLGYRIDGIDILDGIDLRLNAGEMVGLIGPNGAGKSS
                     LVRLLAGVEAPTRGKIRFAGEPLDSWPARERARRIGYLAQDAAAHWPLSVEQIVELGR
                     LPYQKWWLQLTAEDRERVEHALRLTETLAYRNRVVTTLSGGEQTLVMLARIFAAEPEL
                     IFADEPVAALDPYHQLHVMEVIRGHAAGNRAAIVVLHDLGLAARFCDRLYLLRHGRVH
                     CSGTVAEVLTREHIAEVYGVDSRIVDDEQGISFTSLSRLSDQHP"
     gene            complement(2489..3952)
                     /locus_tag="VIW27_12845"
     CDS             complement(2489..3952)
                     /locus_tag="VIW27_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY5740608.1"
                     /translation="MTASTIMIQGCTSDAGKSTLVAGLCRLLVRRGLRVAPIKPQNMA
                     LNSAVTADGGEIGRSQAVQAQACGLDAHSDMNPVLLKPNSDIGAQVIVHGRALTAMDA
                     AGFHDYKRVALDAVLASHRRLAEQYEIIVAEGAGSPAEINLRAGDIANMGFAEAIDCP
                     VILAADIDRGGVFAQLVGTLELLSKTERERTRGFIINRFRGDPALLRDGLDWLERRTG
                     KPVLGVVDYLHGLHLEAEDAIDPGAGVVERGAPTLKIVVPVLPRISNHTDFEVLRLQP
                     EVDFEFVGPGQSLPAADLVILPGSKSVRCDLRFLEQQGWADAIRRHCRYGGKLLGICG
                     GLQMLGSAVHDPHGIESEPGSSTGLSLLELETRLEPEKQLRRVVGRLSLEDAAFDGYE
                     IHAGVSTGPALEQPFAHIGERFDGALSADRQIAGTYVHGLFDVSASRAALLRWAGLEK
                     SHEFDYFELREHSLERLADELQRALDVDRILDWLGKR"
     gene            complement(3980..>5139)
                     /locus_tag="VIW27_12850"
     CDS             complement(3980..>5139)
                     /locus_tag="VIW27_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740609.1"
                     /translation="QGTSVDIPADSLLQETGADPANDLGFELRVNLGERRGDWQWQAD
                     YQLLARKGDRFEAQQQNPELDFGAAAFVDDDTRLFDLTRRISDGDDRVVAHRLDRLYL
                     GYSDVKTVLKFGRQAISWGNGLIYNPVDFFNPFDPAAIDTEYKTGDDMLYGQCLLDSG
                     DDLQALWVLRRDADGDVEREVASLAVKFHGFAAAGEYDLLLAEHYDARNVALGGASSA
                     GDAIWRGDLMLTDSGDETFSSAVLNWSYSWVAWNRNLSAAIEYYRNGFGIDDGDYGPP
                     ALADNPELVERIRRGELFTLGRDYLAATATLELTPLWLLTASLFNNLNDNSRLLQVFS
                     RHDLAQNSQLLLALNLPSGSDGSEFGGIDSGVEGRPLASGNSLFAQLAWYF"
BASE COUNT          810 a         1696 c         1715 g          918 t
ORIGIN      
        1 ttccgctgaa cgacgcgtcg gtggaggcgg tggtcgctta ccagccggac ctggtgctcg
       61 cgagtacctt cgccgcctgg gacacggcgc gcctgttgcg ccggctgggt tacgaagtcc
      121 ggcagattcc gccggtgggc tcgatcgccg atatctacga cctgctgcgc caggtgggcg
      181 actggaccgg caacgcggcg cgcgccgcaa gcctggccgc cgagatgcgg cggcggctcg
      241 ccgatatcga gcggcgctac gcggcgcggc ccgaaaaaac ggtcatcgct tatgccccca
      301 acggctttac ggtgggtggc ggcaccctgg aaaacgatgt cttcgagcac gccgggtacc
      361 gtaacctggc tgccgaaatg ggcatcgagg gctttcagcc gatcgccctc gaaaccctgc
      421 tggccgctga acccgacgtg ctgcagatcg accgcgacct gtcgcgcccg gattcgctgg
      481 ccacggccta tgtcggccac ccggtgctgg agcgggtcag cgagcagcgc gaacagctgg
      541 atattccgct gcgcctgaga atctgtggcg ggccgatgat cgtcgaggcg gtcgaagcga
      601 tggcgcgccg gcggtgaccg cgctgaagcc gagacccgcg gtgctgtatg ccgtgctgct
      661 gccgctgctg ttactggccc tgctgctcgc actgcaggtc gggccggtcg acatcagtct
      721 cgccaaggcc tggcgcgact ggacaggcgg gcagaccacg atcgagaccc tgatcctgtt
      781 cgaactgcgc ctgccgcgcg cgctgctggc gctggccatc ggcgcgaccc tcggtctcgc
      841 cggcgcttcg ctgcagggct tgctgcgcaa cccgctggcc gagcccggca tcatcggcgt
      901 ctccaacggc gcggcgctcg gcgcggtgat cgtattctat tacgggcttg cgggccttgc
      961 gtggttcgtg ttgccgctgg gcggcttgct cggcgcgatg atttcggtgg cggtgatctt
     1021 catgctgatt ggtcgcagtc gttcgatcgt cacgctgatc ctcgccggaa tcgcgatcaa
     1081 cgccatcggc ggcgcgctga tcgcgctggc gctcaatttt gccgacagcc cgtacgcgat
     1141 gcaggaaatc gtcttttggt tgctgggttc ggtcgccaat cgcagcgtca acgaactcta
     1201 catcgcgttg cctttcatgg ttgccggctg gctgctgatg ctctactgcg gccgctttct
     1261 caacgcattg acgctcggcg aagaaaccgc cgcgtcgatg ggcttcgatg tcgcgcgcat
     1321 gcgtctgttg ctggttatcg gcgttaccgc gagtgtcggc gccgcggtct cggtggccgg
     1381 caacatcggc ttcgtcggcc tggtcgtgcc gcacctgctg cgtcccttcg tcgccttcga
     1441 gccgcgacgc ctgctgccgg ccagcatggc cgccggcgcg atcctgctgc tggtcgccga
     1501 catcgccgtg caattgctgt cggagacccg ggaaatgaaa ctcggcgtca tcacctcgct
     1561 ggtcggcgcg ccgtttttcc tgttcctgat cctgaaatcg aggaacatgc tgcaatgaac
     1621 ctgctggaag gcgacaagct cggctaccgg atcgacggta tcgatatcct cgacggcatc
     1681 gacctgcgcc tgaatgccgg tgaaatggtc ggcttgatcg gccccaacgg cgccggcaag
     1741 agcagcctgg tgagactgct ggccggagtc gaggcgccga ctcgcggcaa gatacgcttc
     1801 gccggcgagc cgctcgacag ctggccagcg cgtgagcgcg cgcggcgcat cggctatctg
     1861 gcgcaggacg ccgccgcaca ctggccgctc agcgtcgaac aaatcgtcga actcggccgc
     1921 ctgccttacc agaaatggtg gctgcaactg accgccgaag accgcgagcg ggtcgaacac
     1981 gcgctgcggc tgaccgagac gctggcctac cgcaatcgcg tcgtcactac cttgtcgggg
     2041 ggcgagcaga cgctggtcat gctggcgaga atattcgccg ccgagccgga gttgatcttc
     2101 gccgacgagc cggtcgccgc gctcgatccc tatcatcaat tgcacgtcat ggaggtgatc
     2161 agaggccatg ccgccggcaa ccgtgcggcg atcgtcgtgc tgcacgatct cgggctcgcg
     2221 gcgcgctttt gcgaccggct ctacctgttg cgccatggcc gcgtgcactg cagcggcacg
     2281 gtcgccgagg tgctgacccg cgagcatatt gccgaggttt acggagtcga ctcgcgcatc
     2341 gtcgacgacg agcagggtat cagctttacc tcgttatcga ggctttccga ccagcatccc
     2401 tagcgacggg catgcgcagc ggtctgctgc cggaagtgcc cctggcgcgg gtcgatgcaa
     2461 gcgcgggccg tccccgtagt tcgcgggatt agcgcttgcc cagccagtcg agaatgcggt
     2521 cgacgtcgag cgcgcgctgc agttcgtcgg cgaggcgttc gagcgagtgt tcgcgcagct
     2581 cgaagtagtc gaactcgtgc gacttctcga ggccggccca gcgcagcagc gccgcgcggc
     2641 tcgccgacac gtcgaacagg ccgtgcacgt aggttcccgc gatctggcgg tccgccgaca
     2701 gcgcgccgtc gaaacgctcg ccgatgtgcg cgaacggctg ctcgagcgcg gggccggtgg
     2761 aaacgccggc atgaatttcg taaccgtcga aggcggcatc ctcgagcgat agccggccga
     2821 cgacccttcg cagctgcttt tccggctcga gccgcgtctc gagttcgagc aggctgagtc
     2881 ccgtgctact gccgggttcg ctctcgatac cgtgcggatc gtgcaccgcg ctgccgagca
     2941 tttgcagccc gccgcagatg ccgagcagct tgccgccgta acggcagtga cggcgaatcg
     3001 cgtcggccca gccctgctgt tccagaaagc gcaggtcgca acgcaccgac ttgctgccgg
     3061 gcaggatcac cagatccgcc gccggcaaag actggcccgg gccgacgaac tcgaagtcga
     3121 cctcgggctg cagccgcagt acctcgaaat cggtgtggtt gctgatcctc ggcagtaccg
     3181 gcaccacgat tttcagcgtt ggcgcgccgc gctcgacgac cccggcgccg gggtcgatcg
     3241 cgtcctcggc ctcgaggtgt agcccgtgca ggtaatcgac cacgccgagc accggcttgc
     3301 cggtgcgccg ttctagccag tcgagaccgt cccgtaacag tgcagggtca ccgcgaaagc
     3361 ggttgatgat gaagccgcga gtgcgctcgc gctccgtctt cgacaacagc tcgagcgtgc
     3421 cgaccagctg cgcgaaaacg ccgccgcggt cgatgtcggc ggccaggatc accgggcagt
     3481 cgatcgcctc ggcgaagccc atgttggcga tgtctccggc acgcaggttg atctcggccg
     3541 ggctgccggc gccctcggcg acgatgattt cgtactgctc ggcgagccgg cgatgggacg
     3601 cgagcaccgc gtcgagggcg acgcgcttgt aatcgtgaaa accggccgcg tccattgccg
     3661 tcagcgccct gccgtgaacg ataacctgcg cgccgatgtc gctgttgggt tttagcagca
     3721 ccggattcat gtcgctgtgc gcatcgaggc cgcaggcctg tgcctgtacc gcctgcgagc
     3781 gcccgatttc accgccgtcg gcggtgaccg cgctgttcag cgccatgttc tgcggtttga
     3841 tcggcgccac ccgcaggccg cgccgtacca gcagacggca caggccggcg accagcgtgc
     3901 tcttgccggc atcggaggtg cagccctgga tcatgatggt gctggcggtc atcgacgacc
     3961 ccgtgaaacc ggctccatcc tagaaatacc aggccagctg ggcaaacagc gaattgccgc
     4021 tcgccagcgg ccgtccttcg accccggaat cgatgccgcc gaactcgctg ccgtcgctgc
     4081 cgctcggcag gtttagcgcc agcaacaact gcgaattctg tgcgaggtca tgccgtgaaa
     4141 acacttgcag caggcgcgaa ttgtcgttca gattgttgaa caggctcgcc gtcagcagcc
     4201 acagcggtgt cagttccagg gtggcggtgg cggcgaggta atcccgcccc agcgtgaaca
     4261 gttcgccgcg gcgaatgcgc tcgaccagtt cgggattgtc ggccagcgcc ggcggtccgt
     4321 agtcaccgtc gtcgatgccg aagccattgc ggtagtactc gatcgcggca ctcaggttgc
     4381 gattccacgc gacccacgaa tacgaccagt tgagcaccgc gctgctaaag gtctcgtcgc
     4441 cgctgtcggt gagcatcagg tcgccgcgcc agattgcgtc gccggcgctc gaggcaccgc
     4501 cgagcgcgac attgcgggca tcgtaatgct cggccagcag caggtcgtac tcgccggcgg
     4561 cggcaaaacc gtgaaacttg accgccaggc ttgcgacctc gcgttcgacg tcgccgtcgg
     4621 catcgcgccg caatacccac agggcctgca ggtcgtcacc ggagtcgagc aggcattgcc
     4681 cgtagagcat gtcgtcgccg gtcttgtact cggtatcgat cgccgccgga tcgaaagggt
     4741 tgaagaaatc gaccgggttg tagatcagcc cgttgcccca ggagatcgcc tggcggccga
     4801 acttgagcac ggttttgacg tcgctgtaac cgaggtagag ccgatcgagc cgatgcgcga
     4861 cgacgcggtc gtcaccatcg ctgatgcgcc gggtcagatc gaacagccgc gtgtcgtcgt
     4921 cgacgaacgc ggcagcgccg aaatcgagct cggggttttg ttgctgcgcc tcgaagcggt
     4981 cgcccttcct ggcgagcaac tggtagtcgg cctgccattg ccagtcgccg cggcgctcgc
     5041 cgagattgac gcgcagctcg aagccgaggt cgttggcggg atcggcgccg gtctcctgca
     5101 gcaggctgtc ggctggaata tcgaccgagg tcccctgca
//
LOCUS       DATCZV010000568         2225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_628871, whole genome shotgun sequence.
ACCESSION   DATCZV010000568 DATCZV010000000
VERSION     DATCZV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628871"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VIW27_12855"
     CDS             <1..195
                     /locus_tag="VIW27_12855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017444077.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5740610.1"
                     /translation="AVNEGYLDGIGVAKVQDYEAALHDYAHANNADLLEKINETGAYD
                     DEIEASLKALCEGFAEKGAY"
     gene            198..1064
                     /gene="atpG"
                     /locus_tag="VIW27_12860"
     CDS             198..1064
                     /gene="atpG"
                     /locus_tag="VIW27_12860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY5740611.1"
                     /translation="MPSEKEIRNKIRSVKNMQKITKAMEMVAASKIRKAQAQMEASRP
                     YAERIRRVIGHLSYANPDYKHPWLVEREARRVGCIVISTDRGLCGGLNVNMFKTVIGE
                     LQRWQEQNVEVDLALVGAKAVQFFRRLGGNVVGTATHLGDRPQVNDLIGAIKIMLDSY
                     SEGNIDRVFLVHNEFVSTMSQRPEVKTLLPVSAIDLGDEKLQAHWDYIYEPDAIDLLD
                     DVLSRYIESQVYRGAVENFACEMAAKMIAMKSATDNAGEIIDKLQLDYNKARQAAITQ
                     EISEIVGGAAAV"
     gene            1083..2225
                     /gene="atpD"
                     /locus_tag="VIW27_12865"
                     /pseudo
     CDS             1083..2225
                     /gene="atpD"
                     /locus_tag="VIW27_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313309.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
BASE COUNT          515 a          658 c          665 g          387 t
ORIGIN      
        1 gcggtaaacg agggctacct ggatggcatc ggcgtcgcca aggtccagga ctatgaagct
       61 gcgttgcacg actatgcgca tgcaaataat gcggaccttc tcgaaaaaat taacgaaacc
      121 ggcgcgtacg atgatgaaat cgaagcgagt ctaaaagcac tctgcgaggg tttcgcggag
      181 aaaggtgctt actagaaatg cctagcgaaa aagaaattcg caacaagatc cgaagcgtga
      241 aaaacatgca gaagatcacc aaagccatgg aaatggttgc tgcatcgaag attcgcaagg
      301 cgcaggcgca aatggaagcc tcgcgcccct acgccgagcg tatccgtcgc gtgatcgggc
      361 atctttcata cgccaacccg gattacaagc atccctggct ggtggagcgt gaagcgagac
      421 gcgtcggctg catcgtcatc tcgaccgacc gcggcctctg cggcggactg aatgtcaaca
      481 tgttcaagac cgtcatcggc gagttgcagc gctggcagga acagaacgtc gaggtcgatc
      541 ttgcgctggt cggcgccaaa gccgtgcagt ttttccggcg cctcggcggc aacgtcgtcg
      601 gcacggcaac ccacctcggg gatcggccgc aagtcaatga cctgatcggt gcgatcaaga
      661 tcatgctgga ctcgtacagc gaaggaaata tcgatcgcgt gttcctcgtg cacaacgaat
      721 tcgtcagcac catgagccag cggccggagg tcaagacctt gttgccggtc agcgccatcg
      781 acctcggcga cgaaaagctg caggcacact gggactacat ttacgagccc gacgccatcg
      841 atctgctcga cgatgtgctg tcgcgttata tcgagtcaca ggtctacagg ggcgcggtcg
      901 aaaactttgc ctgcgaaatg gcggcaaaaa tgattgccat gaaatcggcg accgataacg
      961 cgggtgaaat cattgacaag ctgcagctcg actacaacaa ggcccgtcag gctgcgatca
     1021 cacaggaaat ttcagaaatt gtcggtggcg cggctgccgt ttgagattag aggactgagt
     1081 acatgagcac aggaaccacc gtgcaagtca ttggcgccgt tgtcgacgtc gaattcccga
     1141 ccgatgccat tcccaaagtt tatgatgcgc tgttgatcga tgacgccgat ctgacgctcg
     1201 aagtgcagca gcagctgggc gacggcgtcg tacggacgat cgcgatgggc tcgagcgagg
     1261 gcctgaagcg cggcatgggc gtaagcaata ccggcgggcc gatcacggtt ccagtcggtg
     1321 agaagaccct cggccgcatc atggacgtgc tcggcaaacc gatcgacaac gccggcgata
     1381 tcggtgccga gacgcacttg cccattcaca ggaaggcgcc gacctacgag taacaggcgg
     1441 ctgccacaga gcttttagaa accggcatca aggtcatcga cctcatcatg ccgatcgcca
     1501 agggcggcaa gatcggcctc ttcgggggtg ccggtgtggg caagaccgtg tgcctgatgg
     1561 agctgatcaa caacatcgcc aaggaacacg gtggctattc ggtgttcgcg ggcgtcggcg
     1621 agcggacccg cgaaggcaat gacttctacc acgagatgac ggacggcggc gtcatcgaca
     1681 aggtatcgct ggtctacggg cagatgaacg agccgccggg caaccgcctg cgcgtcggcc
     1741 tcaccggcct gacgatagcg gaagcgttcc gcgacgaggg ccgcgacgtg ctgatgttca
     1801 tcgacaacat ttaccgctac acgctcgccg gcaccgaggt gtcggcgctg ctcggccgca
     1861 tgccgtcggc cgtgggctac cagccgacgc tggccgagga aatgggcgtg ctgcaggagc
     1921 gcatcacctc gaccaagacc ggctcgatca cctcgttcca ggcgatctac gttcccgcgg
     1981 acgacctgac agacccgtct ccggcaacca ccttcgcgca cctcgacgcg acactcgtcc
     2041 tgtcgcggca gatcgccgag ctcggtatct acccagcggt cgacccgctc gattccacga
     2101 gccgtatcct cgatccgaac atcatcggcc agcgtcatta cgacgtcgcc cagaacgtca
     2161 agaagattct ccagacctac aaggacctgc aggatctgat caacatcctc ggcatcgacg
     2221 aactc
//
LOCUS       DATCZV010000569         2896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_734553, whole genome shotgun sequence.
ACCESSION   DATCZV010000569 DATCZV010000000
VERSION     DATCZV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734553"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="VIW27_12870"
     CDS             complement(<1..1765)
                     /locus_tag="VIW27_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5740612.1"
                     /translation="MNAVVGERQIPGRLAYLWQEQRGWVLTAGLLLCSLLVWQLQGGD
                     TVFPADWSAAFPFASTVDAFDAWVRPYVQPTTRAIAAVVIWLYELLVDFLIFTQWQVV
                     FVILVLPAFAYGGLRLGLLAIIAVGSWLVLDFWDESMETLSLMSISILISIVIGVLMG
                     IAASQNDRVEAVIKPILDTMQTLPAFVYLIPAFYLFGIGPPGAILATVIYALPPIVRL
                     TNLGIRQVPAGIDEAATSFGATRAQSLVKVKLPLAMPSIKLGMNQTVMMALALVVLAT
                     FIGSPGLGDIVYQGLVRLNVGKALEGGLAIVLMAVLLDRVTYAMGHREHVSASRYDHE
                     FRLFPQRLDKYRLVVYVEKAIDWLWRQLVAAGNFVTLAVSVAVCGLLAPLNADLATRL
                     REIMLARSFLIASLVLIAAMLLIESYTDLFGKFPRDWEYRFRTPVNQYMDELVADKLF
                     YAITQSIKEGLYYGLINPLNTFLKGLPWWYTSAVFTLVAWLVSGRGLAIATLLGFLFI
                     GATDLWSFGMITLTTVIVSVFICFLIGVPLGILAAYNKTADTLIKPVLDTMQTMPVFV
                     YLVPVIMLFGAGQVPAVIATVI"
     gene            1904..>2896
                     /locus_tag="VIW27_12875"
     CDS             1904..>2896
                     /locus_tag="VIW27_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5740613.1"
                     /translation="MTDSPKIVCNNVWKIFGTYPERTLQTIDRSLSRAEVQQQTGHVI
                     AVKDVSFEIRKGETFVVMGLSGSGKSTLVRCISRLIEPTAGQMIIDGEDIMAYNDAQL
                     TELRRFRMSMVFQHFGLFPHRKVIDNISFGLEIRGMDRRARNAKAMEVLEKVGLHGWA
                     NHYPRELSGGMQQRVGLARAMAVDPEILIFDEPFSALDPLIRREMQDELIKIQNEVHK
                     TMIFITHDFLEAIKMGDHIAIMKDGEVVQIGTPEEIVANPVDDYVREFTEDVPRYKVL
                     SVGKIMRAPSAAVHADGTCCQVGAKLDSLIDLAIETAGPLGVLDDGGRLVGEIDQ"
BASE COUNT          642 a          883 c          886 g          485 t
ORIGIN      
        1 agatcaccgt ggcaatcacc gccggcacct ggccggcgcc gaacagcatg attaccggca
       61 ccaggtagac gaataccggc atcgtctgca tggtgtcgag aaccggcttg atcagggtat
      121 cggcggtctt gttgtaagcc gcgagtattc ccagcggcac gccgatcaga aaacagatga
      181 acacggacac gataaccgtg gtcagcgtga tcatgccaaa gctccacagg tcggtggcgc
      241 cgataaacag gaatcccaac agggtcgcga tcgccagccc gcggccgctt accagccacg
      301 cgacgagggt gaatacggcg ctcgtatacc accagggcaa acccttcaga aaggtgttca
      361 gcggattgat caaaccgtaa tagaggccct ccttgatcga ctgggttatc gcgtaaaaca
      421 gcttgtcggc gaccagttcg tccatgtact ggttgacggg agtacgaaat cggtattccc
      481 agtcgcgcgg aaatttgccg aaaagatcgg tgtaggattc gatcagcagc atcgccgcga
      541 tcaataccag gctggcgatc aggaaactgc gcgccagcat gatttcgcgt aaccgcgtgg
      601 cgagatcggc gttgagcggc gccagcagcc cgcagacggc gacgctgacc gccagggtga
      661 cgaaatttcc ggctgccacc aactgccgcc agagccagtc gattgccttt tcgacgtaga
      721 ctaccaggcg atacttgtcg aggcgttggg ggaacagccg aaactcgtga tcgtagcggc
      781 tcgcgctgac atgctcgcgg tgtcccatcg cgtaggtgac gcggtcgagc aataccgcca
      841 tcagcacgat agccaggccg ccttccagcg ccttgccgac gttgagtcgc accagtccct
      901 ggtaaacgat atcgccgagg cccggggaac cgatgaaggt cgccagcacc accagcgcca
      961 gcgccatcat taccgtctgg ttcatgccga gcttgatcga cggcatcgcc agcggcaact
     1021 tgaccttgac cagggattgg gcgcgagttg caccgaacga ggtcgcggct tcatcgatgc
     1081 cggcgggcac ctggcgaatg ccgagattgg tcaggcgcac gatcggcggc aacgcgtaga
     1141 tgacggtcgc caggatggcg ccgggcggac cgatgccgaa caggtagaaa gccgggatca
     1201 ggtagacgaa cgccggcagc gtctgcatgg tatcgagaat cggcttgatc accgcctcga
     1261 cgcgatcgtt ctgcgacgcg gcgataccca tcaatacgcc aatcacgatc gagatcagga
     1321 tcgagatact catcaggctc agggtttcca tcgattcgtc ccagaaatcg agcaccagcc
     1381 acgaaccgac ggcgattatc gccagcaggc cgagacgcag gccgccgtag gcgaaagccg
     1441 gcagcaccag gatgacgaat accacctgcc actgggtaaa aatcaggaaa tcgaccagca
     1501 attcgtagag ccagatcacc accgcggcga tggctcgcgt ggtcggctgc acataggggc
     1561 gaacccaggc atcgaatgca tcgaccgtgc tggcgaacgg aaacgcggca ctccagtccg
     1621 cgggaaagac ggtgtcgccg ccctgcagtt gccagaccag caacgagcac agcagcaggc
     1681 ccgcggtgag cacccagccg cgctgttcct gccacagata tgcgagtcgc ccgggtatct
     1741 gccgctcccc caccacagcg ttcatcggtc agagccacgt aaagagatgc gggctagctt
     1801 agcgtcgatc gcgcaggccc gcaaccggct cgaggcgtcg gtttacaccg ctttcgggtt
     1861 cgggctaaga tactcggcga aggtgcgaat aacgagtacg acaatgaccg attcccccaa
     1921 gatcgtctgc aacaatgtct ggaagatatt cggcacctat cccgagcgga ctctgcaaac
     1981 catcgaccgc tcgctgtcgc gcgccgaggt ccagcagcag accggtcacg tgatcgcggt
     2041 caaggacgtt tccttcgaga tcaggaaagg cgaaaccttt gtcgtcatgg gtctgtcggg
     2101 cagcggcaag tcgaccctgg tgcgctgtat ctcgcgtctg atcgagccca ccgccgggca
     2161 gatgatcatc gacggcgaag acatcatggc atacaacgat gcacagctga ccgagctgcg
     2221 ccgttttcgc atgagcatgg tattccagca cttcggcctg ttcccgcacc gcaaggttat
     2281 cgataacatt tccttcggcc tcgaaattcg cggcatggat cgcagggcgc gcaacgcgaa
     2341 ggccatggaa gtgctcgaaa aagtcggcct gcacggctgg gccaaccact atccgcgcga
     2401 actctcgggc ggtatgcagc agcgcgtcgg cctcgcgcgc gcgatggcgg tggatcctga
     2461 aatcctgatc ttcgacgagc cgttcagcgc gctcgacccg ctgattcggc gcgaaatgca
     2521 ggacgagctg atcaagatcc agaacgaagt gcacaagacg atgatcttca tcacccacga
     2581 tttcctcgag gcgatcaaga tgggcgatca catcgcgatc atgaaggacg gcgaggtggt
     2641 gcagatcggc actcccgagg aaatcgtcgc caacccggtg gacgattacg tgcgcgaatt
     2701 caccgaggac gtgccgcgtt acaaggtgct ctcggtcggc aagatcatgc gcgcgccgtc
     2761 tgccgcggta cacgccgacg gcacctgctg ccaggtcggg gccaagctcg attcgctgat
     2821 cgacctggcg atcgaaaccg cgggtccgct gggcgtgctc gacgacggcg gcaggctggt
     2881 cggcgagatc gaccag
//
LOCUS       DATCZV010000570         5370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_681562, whole genome shotgun sequence.
ACCESSION   DATCZV010000570 DATCZV010000000
VERSION     DATCZV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681562"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..694)
                     /gene="arsN2"
                     /locus_tag="VIW27_12880"
     CDS             complement(227..694)
                     /gene="arsN2"
                     /locus_tag="VIW27_12880"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEY5740614.1"
                     /translation="MAIDATAAPADAIVRLNPSQLPELRALLEANGLPADDCAEQAEN
                     FYGIVERGRLIAAGGLQPAAQYFLLRSLVVAESCRGRGLARALTEFLLARAAAAGSPA
                     VYLLTENAADYFAGLGFEDLARERVPAAIRQTRQFSSLCPDDARCLVIGPCRG"
     gene            complement(684..2000)
                     /locus_tag="VIW27_12885"
     CDS             complement(684..2000)
                     /locus_tag="VIW27_12885"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY5740615.1"
                     /translation="MKPVKLGLLGVGTVGASTALVLKNNAAEIARRAGRSIEIIQASR
                     RDISAGLPPGSGDIELKQDPFEVVDNPDVDIVIELIGGTDTARELVMRAIANGKHVVT
                     ANKALIALHGNEIFAAAQARGVNVVFEAAVAGGIPIIKSIREGLAANHIEWLAGIING
                     TGNFILTEMRDAGRSFDDVLLEAQALGYAEADPTYDVEGIDAAHKLCILSSIAFGIPL
                     QFDAVYTEGISGISTEDVVYAGQLGYRIKHLGVARRGEQGIEMRVHPTLIPQKRLIAN
                     VDGVMNAVLVQADKLGPSLYYGAGAGADPTASAVIADIIDVARTITTDAQNRVPHLAF
                     QPDQIVDIPILPMAEVQTAYYLRMRVDDRPGVMAEITRIMGDKDISIEAILQKEPEPG
                     VTRATIIMLTQKIRERQMDEAIAAIAELDAVHGEVHRIRVETLDGH"
     gene            2131..2505
                     /locus_tag="VIW27_12890"
     CDS             2131..2505
                     /locus_tag="VIW27_12890"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEY5740616.1"
                     /translation="MPLAEDRVTSRHRITAYGPQTITVNETVYHQSLVLTADTLLSPW
                     PVTSLEELDGEALQPLLASNPAVVLLGTGQQQRFPEARLYGLFGQRGIGLEVMDNGAL
                     CRTFNILVAEDRAVTAAIIIGD"
     gene            complement(2506..3519)
                     /gene="rpoS"
                     /locus_tag="VIW27_12895"
     CDS             complement(2506..3519)
                     /gene="rpoS"
                     /locus_tag="VIW27_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016916301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEY5740617.1"
                     /translation="MTVMPDGIDDDDDEDDDDDIEHASVVDEDDADEDDTTIQAEKFV
                     ERRKPEDRRKVSTRRSSDADPTRLYLKEIEVSPLLTAEEEVYYSRLALKGDTAARDKM
                     IECNLRLVVKIARRYMNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQ
                     TIERGLMNQTRTIRLPIHVIKELNTYLRAARKLTQELNREATPEDVARLLKKPVKDVE
                     KMFKLADRVSSFDIPMGGDGERPLLDVIPDEGGVDPASILQDETVIKHLDSWLDELDE
                     KQRDVVVRRFGLRNHPRGTLEEVGLELGVTRERVRQIQMDALRKLRRILEHSGYSREN
                     IIE"
     gene            complement(3619..4329)
                     /locus_tag="VIW27_12900"
     CDS             complement(3619..4329)
                     /locus_tag="VIW27_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5740618.1"
                     /translation="MRTALVFIVAVTLLASCVTVKPPIHVPRGPGWYTVKRSDTLYSI
                     AWRYGLDYKELARWNSININQPIYPGQRLRLIRPADTAVVVREPAAEPAPSAPPQANE
                     KPPPSTQEIGPDPRAWLWPTDGKPLNTFLASDLDRRGIDIAGEPGQPVRAVAAGRVVY
                     SGNGLAGYGNLIIIKHSDTFLSAYAYCQERLVQEGVSVKAGDLVAKMGRRDNQAKLYF
                     EIRRNGKPVDPIKYLPDL"
     gene            complement(4348..4929)
                     /locus_tag="VIW27_12905"
     CDS             complement(4348..4929)
                     /locus_tag="VIW27_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY5740619.1"
                     /translation="MQVFEPAYNAVIRFSRRSDAPYYLALLSFLESFILPFPPPDVML
                     APMSIAQPRRALRFAALTLAFSVFGGLVGYLIGAFLFDLAEPLIVDWGYQSRFETVIA
                     WFDEWGFWAVLVAGFSPVPYKIFTIAAGVLNLALLPFLLASIIGRGARFFLVAWCLAR
                     FGPAIESRLMRYIEIVGWAVVAALLLAIVIYSF"
     gene            complement(4929..>5370)
                     /locus_tag="VIW27_12910"
     CDS             complement(4929..>5370)
                     /locus_tag="VIW27_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5740620.1"
                     /translation="AQMTELLLQDGPANSVLEIGTGCGYQTAVLASLFPQVYSVERIE
                     ALHQQAKKRLAELEFYNVQLRHTDGIEGWPNISYRFDRILMTAACSELPEALAAQLAD
                     GGVMVLPLDDGNGQTMTRITRTRDGLVSESLGKVIFVPLLPGLA"
BASE COUNT          981 a         1733 c         1650 g         1006 t
ORIGIN      
        1 agaaaatctg cccgccggcc atgccggcga aaaacagcgt aatcacgtac tggctgtcgt
       61 tgaggcggga gacgtcgaga tcgccggcga ttaccggcag cgccggcaac atcgcgtcga
      121 tcgacagcgc gaccatcgac gtcatcagcg caaccagtac cacgaactcg agcgctcccg
      181 gcccttcgcc ggcttccgct tgacggcctg tgtcgccggg cctcactcag ccccggcacg
      241 gcccgatgac caggcagcgg gcatcgtcgg gacataacga ggagaattgc cgggtttgcc
      301 tgatcgccgc gggcactcgc tcgcgcgcca ggtcctcgaa tccgaggccg gcaaaataat
      361 cggcggcatt ttcggtgagc aggtaaaccg cgggactgcc cgccgccgcg gcgcgtgcga
      421 gcaggaactc cgtcagcgct cgtgccagtc cgcgcccgcg gcaggactcg gcgaccacca
      481 gcgaacgcaa caggaaatat tgcgcggccg gctgcaggcc gccggcagca atcaggcgcc
      541 cccgctcgac gatgccgtag aaattttccg cctgctcggc gcaatcgtcg gccggcaggc
      601 cgttggcttc cagcagggcg cgcaactcgg gcagttgcga cggattcagt cggactatgg
      661 cgtccgctgg cgcggctgtc gcatcaatgg ccatcgaggg tttcgacgcg aatgcgatgt
      721 acctcgccat gcaccgcgtc gagctcggcg atcgccgcga tcgcctcgtc catctgccgt
      781 tcgcggatct tttgcgtcag catgatgatg gtcgcgcgcg tcaccccggg ttccggttcc
      841 ttctgcagaa tagcctcgat ggaaatatcc ttgtcaccca tgatacgggt gatttccgcc
      901 atcaccccgg ggcggtcgtc gacgcgcatg cgcaggtagt aggcggtctg tacttcagcc
      961 atcggcagga tcggaatatc gacgatctgg tcgggctgga atgccaggtg cggcacgcgg
     1021 ttctgcgcat cggtggtgat cgtgcgtgcg acgtcgatga tgtcggcgat caccgccgag
     1081 gcggtcggat cggcgccggc gccggcgccg tagtacagcg acggtccgag tttgtcggcc
     1141 tgcaccagca ccgcgttcat gaccccgtcg acgttggcga tcaggcgctt ctgcggaatc
     1201 agcgtcggat gcacgcgcat ctcgatgccc tgctcaccac ggcgcgcgac accgagatgc
     1261 ttgatgcgat agcccagctg cccggcatag acgacgtcct cggtcgagat cccgctgata
     1321 ccctcggtat agaccgcgtc gaactgcagc ggaataccga acgcgatcga cgacaggatg
     1381 cagagcttgt gcgccgcgtc gatgccttcg acatcgtaag tcgggtccgc ctcggcgtat
     1441 cccagcgcct gcgcctcgag caatacatcg tcgaagctcc tgccggcgtc gcgcatctcg
     1501 gtgaggataa agttgccggt gccattgatg attccggcca gccattcgat gtggttggcg
     1561 gcaagtccct cgcggatcga cttgatgatc ggaatcccgc cggcgaccgc ggcctcaaat
     1621 accacgttga cgccgcgcgc ctgcgcggcg gcgaagattt cgttgccgtg cagcgcgatc
     1681 agcgccttgt tggcggtgac cacgtgcttg ccgttggcga ttgcgcgcat caccagctcg
     1741 cgcgcggtat cggtaccgcc gatcaattcg atgacgatat cgacatcggg attatcgacc
     1801 acctcgaacg ggtcttgctt caactcgata tcgccgctgc ccggcggcaa gccggcgctg
     1861 atatcgcgcc tcgaggcctg gatgatctcg atactgcggc cggcacggcg cgcgatttcg
     1921 gcggcattgt ttttcagcac cagagcggtg ctggcgccga cggtgcctac accaagcaaa
     1981 cccagtttca ccggtttcaa tccaagtctc cgaaggtagc cgcggggtga aaaattcgac
     2041 cgcgacacat tatgtaatgt ccctgcggct gtcaacagtc gcaccgcgaa cggcgttgaa
     2101 ccgctcgccg gattgcttta ttctgcgccc atgccactcg ccgaagacag ggtcaccagc
     2161 cggcaccgca tcaccgccta cggcccgcaa accatcaccg tcaacgaaac cgtctaccat
     2221 caaagcctgg tactgaccgc cgacaccctg ctcagcccgt ggccggtgac ctcgctggag
     2281 gagctcgacg gcgaagcact gcagccgctg ctggcgtcga acccggctgt ggtgctgctc
     2341 ggcaccggcc agcagcagcg ttttccggaa gcccggcttt acgggctgtt cggacagcgg
     2401 ggaatcgggc tggaggttat ggataacggc gcgctgtgcc gcaccttcaa tatcctggtt
     2461 gccgaggacc gcgcggttac cgcggcgatc atcatcggcg actgattact cgatgatgtt
     2521 ttcccgcgag tagccgctgt gctcgaggat gcgccgcagc ttgcgcagcg cgtccatctg
     2581 gatctgccgc acacgttcgc gggtcacacc gagctcgaga ccgacttctt ccagcgttcc
     2641 ccgcggatgg ttgcgcaagc cgaagcgccg caccaccacg tcgcgctgtt tttcgtcgag
     2701 ttcgtcgagc cagctgtcga ggtgcttgat gacggtttcg tcctgcagaa tcgacgccgg
     2761 gtcgacgccg ccctcgtcgg gaatcacgtc gagcaatggg cgttcgccat ccccgcccat
     2821 cggaatgtcg aacgacgaaa cgcgatcggc gagcttgaac atcttttcga cgtccttgac
     2881 cggcttcttc agcagcctgg cgacatcttc cggtgtggcc tcgcgattca gttcctgcgt
     2941 cagcttgcgc gccgcgcgca ggtaggtgtt cagttccttg atcacgtgga tcggcaggcg
     3001 aatggtgcgc gtctggttca tcagcccgcg ctcgatggtt tgccgaatcc accaggtagc
     3061 ataggtggag aaccgaaaac cgcgctcggg gtcgaatttc tccaccgctc gaatcaggcc
     3121 gagattgccc tcctcgatca ggtcgagcag ggccagcccg cggttcatgt aacggcgcgc
     3181 gatcttgacc accaaccgca ggttgcattc gatcatcttg tcgcgcgcgg cggtgtcgcc
     3241 ctttagcgcc agccgcgagt aatagacttc ttcctcggcg gtcaacaacg gcgagacttc
     3301 gatttccttc agatacaggc gcgtgggatc ggcgtccgac gagcgccgcg tggatacctt
     3361 gcggcgatcc tcgggcttgc gccgctcgac gaatttttcc gcctgtatcg tggtatcgtc
     3421 ctcgtcggca tcgtcctcgt cgacgacgct ggcatgctcg atatcgtcat cgtcatcctc
     3481 gtcgtcgtcg tcgtcgatac cgtccggcat cacggtcata tcgtcgtcgt cgaccatgtc
     3541 ctcggccagg ccttcggcca aaatatcagt gtcttcgata tccgctttat cggacgactt
     3601 gctcataggg ggattccttt agagatcggg cagatacttg atcgggtcga cgggtttgcc
     3661 gttgcgccgg atttcgaaat acagctttgc ctggttgtcg cggcgaccca tttttgcgac
     3721 caggtctccg gccttcactg acactccttc ctgtaccagg cgttcctgac agtatgcata
     3781 ggcgctcaaa aaagtatcgc tgtgcttgat aatgatcaga ttaccataac ccgccaaacc
     3841 attaccacta tataccaccc tgccggcggc aaccgcgcgc accggctggc cgggttcgcc
     3901 agcgatatcg atgccgcgcc gatcgagatc ggaggcgaga aacgtattca acggcttgcc
     3961 atcggtcggc cacagccatg cacgcggatc cggcccgatc tcctgcgtgc tcggcggcgg
     4021 cttctcgttg gcctgtggcg gcgcggacgg agcgggttcg gcggcgggtt cgcgcaccac
     4081 caccgcggta tccgcgggac ggatcaggcg cagccgctgt cccggataga tcggctggtt
     4141 gatattgatg ctgttccagc gcgcgagttc cttgtagtcg agcccgtacc gccaggcgat
     4201 cgagtagagc gtgtcgctcc gcttgaccgt gtaccagcca ggcccgcgcg gcacgtgtat
     4261 cggcggcttg acggtgacgc aagacgccag cagggtcacg gccacgatga aaactaaggc
     4321 agtcctcatg ttgcgcaaac ccagccctca aaacgaatag atgacgatcg ccagcaacaa
     4381 cgccgcgacc accgcccagc cgacgatttc gatgtagcgc atcagacggc tctcgatcgc
     4441 cggtccgaat ctcgccaggc accaggcgac caggaagaag cgcgcgccgc gaccgatgat
     4501 cgaggccagc aggaacggga gcagcgcgag attcaacacg ccggcggcaa tcgtgaaaat
     4561 cttgtagggc accggcgaaa agccggccac cagcactgcc cagaatcccc actcgtcgaa
     4621 ccaggcgatg acggtttcga agcgcgactg gtaaccccag tcgacgatca gcggctcggc
     4681 aaggtcgaac aggaaggcgc cgatcaggta gccgaccagt ccgccgaaca ccgagaacgc
     4741 cagggtcaac gccgcgaagc gcagcgcgcg ccgcggttgc gcgatcgaca tcggtgccag
     4801 catcacgtcg ggcggcggaa aaggcagaat gaacgactcg aggaaactca gcagcgcgag
     4861 gtaatagggt gcgtcgctgc gccgcgaaaa gcggatcacg gcgttgtatg ccggttcgaa
     4921 tacctgcact aggccagccc cggcaataac ggtacgaaga tgacttttcc gaggctctcg
     4981 ctgaccagtc catcccgggt gcgggtgatt cgggtcatcg tctggccatt gccgtcgtcc
     5041 aacggcaaaa ccatcacgcc accgtcggcg agctgcgccg ccagcgcctc gggcagctca
     5101 ctgcaggccg ccgtcatcag gatgcggtcg aaacggtaac tgatgttcgg ccagccttcg
     5161 atgccgtcgg tgtggcgcaa ctgtacattg tagaattcga gctcggcgag ccttttcttc
     5221 gcctgctggt gcaaggcttc gatgcgttcg acgctgtaca cctgcggaaa caggctcgcc
     5281 agcaccgcgg tctggtaacc gcagccggtg ccgatttcga gcaccgagtt ggcggggccg
     5341 tcctgcagca acagttccgt catctgcgca
//
LOCUS       DATCZV010000571         7338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_310754, whole genome shotgun sequence.
ACCESSION   DATCZV010000571 DATCZV010000000
VERSION     DATCZV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310754"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VIW27_12915"
     CDS             <1..890
                     /locus_tag="VIW27_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY5740621.1"
                     /translation="ALQKGTELVETTTRSIEAELEKPPAAEPASTGETGAETPPQAEP
                     EPQPAVGKKGKMFRDRLKSGGLGPVMITLPGGQFRMGGPSAIVSPDETPRRQVSIRPF
                     AVSVYEVTFEDYYRFAQATRHRKPDNNGWDIKTHPVVDVSWNDAIAYTQWLSTQTGRS
                     YRLLSEAEWEYAARGGTTSSYWWGTKPGSNNAHCFDCRTDYNTSKPAKVGTYKPNPFG
                     LYDTAGNVYEWVHDCYHRNYNGAPTDGSAWLGGDCSVRVVRGGAYRSPANSMRAENRE
                     TFKSGKGQYNVGIRVARDL"
     gene            1191..1862
                     /gene="yrfG"
                     /locus_tag="VIW27_12920"
     CDS             1191..1862
                     /gene="yrfG"
                     /locus_tag="VIW27_12920"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HEY5740622.1"
                     /translation="MIDWQAIETVLLDMDGTLLDLHFDTYFWTEHLPSRYAEIKGLDA
                     ADARELVLARIRSNQGTLNWYSTDYWSETLEIDVVELKHEISHKVAVRPYCIDFLDAL
                     RAAGKDVVMVTNAHQDSLDLKMQKTRIADKFHRLITVHEFALPKEDPQCWHEVHRLHP
                     FRAEKTLLIDDNLLALQSAREYGIAALLAIYQPDSRAPKREIAGFDAIHSFDEIMPVA
                     EHADD"
     gene            1855..2298
                     /locus_tag="VIW27_12925"
     CDS             1855..2298
                     /locus_tag="VIW27_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY5740623.1"
                     /translation="MTEPRLPALTGGCLCGGVRYRISGPRRDVIVCHCENCRRTHGHQ
                     AAYTSVAQADLELIERQTLDWYHDRSPDTYRGFCKRCGSSLFWDARDGRGRMAVAAGT
                     LDDSLELKTIGHVFVSEAGHYYRIEDGLPQFESGNAGALEGDGGG"
     gene            2420..2929
                     /locus_tag="VIW27_12930"
     CDS             2420..2929
                     /locus_tag="VIW27_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5740624.1"
                     /translation="MLKAEKFDIFRRSVGHVLDIIGEGWSILIIREAFFGTRRFEEFQ
                     SRLGIARNILTARLKNLCANQILDRVPVKEGAKRHEYVLTRKGKEMMPILIALTQWGD
                     KWVFGAGNEPVVFLDREEGQPIARIQVYSARGEVLRPRDIMVRAGPGATRDARERIEE
                     MNKNGELST"
     gene            2910..3584
                     /locus_tag="VIW27_12935"
     CDS             2910..3584
                     /locus_tag="VIW27_12935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5740625.1"
                     /translation="MANSRPKNYECPLGETSCEWLEQLRELRQQVSELSALVATDALT
                     GLYNFRHFKTMLQNEMDRSKRSGIPTSLAMVDLDHFKRVNDDYGHEVGNQALQHMADI
                     LMSEVRTTDIVCRYGGEEFAMIFPETHLNLALKVADRIRLEIASRPLQTEHGEIKITA
                     SMGLSVYMKTSILDIDDFIESVDKYLYEAKQSGRNCICHVDYNDLRKVTEIGSVERAL
                     LFDDKI"
     gene            complement(3879..4415)
                     /gene="ppa"
                     /locus_tag="VIW27_12940"
     CDS             complement(3879..4415)
                     /gene="ppa"
                     /locus_tag="VIW27_12940"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY5740626.1"
                     /translation="MILDRVSSGKNVPNDINVIIEIPSHSDPVKYEVDKDTGAMFVDR
                     FMGTAMHYPCNYGYVPQTLSEDGDPVDVLVVTPIPVISGAVIRVRPLGMLCMTDEAGK
                     DAKIVAVPIDKLSGLYTHMDTVRDLPRALLDSIAHFFDHYKDLEPGKWVKIDGWVEAE
                     EAKREILESIERYKAENG"
     gene            complement(4513..5769)
                     /locus_tag="VIW27_12945"
     CDS             complement(4513..5769)
                     /locus_tag="VIW27_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY5740627.1"
                     /translation="MSKKNAFYAQSGGVTSVINASACGVIETCRGHGDRIGNVLAGRN
                     GIIGALTEDLIDTSKESDADIAALRKTPSGAFGSCRFKLKGLEENRREYERLIEVFRA
                     HDIGYFFYNGGGDSADTCYKVSQLSETMGFPVQAIHVPKTVDNDLPITDNCPGFGSVA
                     KYIAVSALEASFDVASMAKTSTKVFILEVMGRHAGWIAAAGGLIEDYGVPVLVLFPEI
                     DFDRERFIAAVKQNVERHGYCTVVVSEGARNADGSFLAEQGTRDAFGHAQLGGVAPVV
                     AEIVREALGYKFHWAVADYLQRAARHLASATDVEQAYELGKVSVELALDGENAIMPTI
                     ERISSNPYSWRIGRAPLAEVANVEKPMPRDFITDDGFGITAQCREYLYPLIQGEDYPD
                     YDERGMPRYVVLKNQAVEKKLAEFKL"
     gene            complement(5893..>7338)
                     /locus_tag="VIW27_12950"
     CDS             complement(5893..>7338)
                     /locus_tag="VIW27_12950"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY5740628.1"
                     /translation="DLVGKVEAGIPEDDPRNPGVIADNVGDNVGDVAGMGSDIFESYC
                     GSMIASIAIASTMTLAVADEFGGQENMMFLPLALASLGLLCSIAGILIVKMNSAKSPE
                     KALRTGTIGSSILFIALAYFLIWALGLVSGIWVAVLAGAVGGIVIGLITEYYTAGKPV
                     EHIAKSGETGPATVMISGLAIGMQSVAIPILTICGIIWISSHFAGLYGVGIAAVGMLA
                     TVGITMAIDAYGPVADNAGGIAEMAGLGADTRKITDSLDELGNTTAAIGKGFAIGAAA
                     LAALAIITAYIETVSLNIENFELVLNSPTVLIGVFIGGVIPFVIASITMTAVGDAAFE
                     MIKEIRRQFREIDGLLEGKAEPDTAQCVDIATTAALKKMIIPGTVAVSAPPLVGFLIG
                     PEALGGMLAGALLSCVLMALMMANAGGAWDNAKKTIESGAHGGKGTDAHKAAVVGDTV
                     GDPFKDTSGPSMNILINVMAIVSLVIAPLLG"
BASE COUNT         1467 a         2266 c         2253 g         1352 t
ORIGIN      
        1 atgcgttgca aaagggcacc gaactggtcg agaccacgac ccggagtatc gaggcggagc
       61 tcgaaaagcc gccggcggcc gagccggcgt cgaccggcga aaccggggcc gaaaccccgc
      121 cgcaggcgga gcccgagccg cagccggcag tcggcaagaa gggcaagatg tttcgcgatc
      181 gcctgaagtc gggcggcctg ggcccggtaa tgatcacact gccgggcggc cagtttcgaa
      241 tgggcggccc gtcggctatc gtttcgcccg acgaaactcc gcgccgccag gtaagcatcc
      301 gcccgtttgc ggtatcggtg tacgaggtca ccttcgagga ttattaccgt ttcgcgcagg
      361 ccactcgtca ccgcaagccc gataacaatg gctgggacat caagacccac ccggtggtcg
      421 atgtttcgtg gaacgacgcc atcgcctaca cgcaatggct cagcacgcaa accggcaggt
      481 cgtaccgact gctgtccgag gccgagtggg aatatgccgc gcgtggcggc accaccagtt
      541 cctactggtg gggcaccaag cccggtagca acaacgccca ttgctttgac tgcaggaccg
      601 attacaacac cagcaagccg gccaaggtcg gcacgtacaa gccaaacccg ttcggcttgt
      661 acgataccgc cggtaatgtc tacgaatggg tacacgattg ctaccaccgc aattataatg
      721 gtgcgccaac cgacggctcg gcctggttgg gcggcgattg cagcgtgcgc gtggtgcgcg
      781 gcggcgctta ccgcagcccg gcgaactcga tgcgcgcgga aaatcgtgaa accttcaaga
      841 gtggcaaggg tcagtacaat gtcggtatac gggttgcgcg cgacctgtag agcgagcgtg
      901 tcgcgggtag tcagctgatt gcccgcgccg aacgagtggc tttcgacagc ccgttgaacg
      961 gaagaagcaa tttgtggcca gtgccagaaa aacccggtgc acggctcgtg taaccggccc
     1021 tcactcggtt ccggcgccag cgggtcgaac cgccgcgcgg tctgctattc gccgctgccg
     1081 gctcatggcc agccgtcgcc atgatcgtgg cctgcggtcg gcaaggccgc tacgcaacga
     1141 ctgtcccgat ccgtcacccg cagcgcagat gcggtattcg ggctagcgcc atgatcgact
     1201 ggcaggccat cgaaacagtg ctgctggaca tggatggcac gctgctggat ctgcatttcg
     1261 acacctattt ctggaccgag cacctgccgt cgcgctacgc cgagatcaag ggcctcgatg
     1321 ccgccgatgc ccgcgagctt gtcctggccc ggattcgcag caaccaggga acgctgaatt
     1381 ggtactcgac cgattattgg agcgagacac tggagatcga cgtcgtcgag ctcaaacacg
     1441 aaatcagcca caaggtcgca gtgcggccgt actgcatcga ctttctcgat gcactgcgcg
     1501 ccgccggcaa ggacgtggtg atggtcacca atgcacacca ggacagcctc gacctgaaaa
     1561 tgcaaaagac tcgaatcgcc gacaaattcc accgcctgat cacggtgcac gagttcgcgc
     1621 tgcccaagga ggatccgcag tgctggcacg aagtgcatcg gctgcatccg ttccgcgccg
     1681 aaaaaaccct gctgatagac gataacctgc tggccctgca gtcggcacga gaatacggca
     1741 ttgccgcgct gctggcgatc taccagcccg actcgcgcgc gccgaagcgt gaaatcgccg
     1801 gcttcgacgc gatccattcg ttcgacgaaa tcatgccggt agcggaacac gccgatgact
     1861 gaaccaaggt tgccggcgct caccgggggc tgcttgtgcg gcggcgtgcg ctaccggatt
     1921 agcggtccgc ggcgcgacgt catcgtctgc cattgcgaaa attgccggcg cacccacggc
     1981 caccaggccg cctatacctc ggtcgcgcag gccgacctgg agctgatcga gcggcaaacg
     2041 ctcgactggt accacgaccg ctcgcccgat acctaccgcg gtttctgtaa gcgctgcggc
     2101 tccagcctgt tctgggatgc ccgtgacggg cggggcagga tggcggttgc cgcgggcacg
     2161 ctcgacgaca gcctcgaact caaaaccatc ggtcacgttt ttgtctccga ggccggccat
     2221 tattatcgaa tcgaagacgg actgccgcaa ttcgaatccg gtaacgcggg cgcgctcgaa
     2281 ggtgacggcg gcggttgacc tcggggcggg aatgcgttaa aagtttcaat cgacaactat
     2341 aattactgtt ttgcgttcgt ccgctacggg ccgcggccgg aatcgggcat tgcgaaaacg
     2401 acacccagag cgcacaacca tgttaaaagc cgagaaattc gatatcttca ggcgctctgt
     2461 cgggcatgtg ctcgatatca tcggcgaggg ttggtcgatt ctgatcattc gcgaggcgtt
     2521 tttcggcacc cgtcgcttcg aggagttcca gagccgcctc ggtatcgcgc gcaacattct
     2581 caccgcgcgg ctcaagaacc tgtgcgccaa ccagattctc gatcgcgtgc cggtcaagga
     2641 aggcgccaag cgccacgagt atgtgctgac ccgcaagggc aaggaaatga tgccgatact
     2701 gatcgcgttg acgcaatggg gcgacaagtg ggtattcgga gcgggcaacg agccggtggt
     2761 atttctggat cgcgaagagg gacagccgat tgccagaatc caggtttact cggctcgcgg
     2821 cgaagtgctg cggccgcgcg acatcatggt cagggcgggt cccggcgcga cgcgtgatgc
     2881 cagggaacgg atcgaggaaa tgaacaagaa tggcgaactc tcgacctaag aactacgagt
     2941 gcccgctcgg cgagaccagt tgcgaatggc tcgagcagct gcgcgaacta cgccagcagg
     3001 tcagtgaact gtcggcgctg gtggccaccg acgcgctgac cggactgtac aatttccgtc
     3061 atttcaagac catgctgcaa aacgaaatgg accgctccaa gcgcagcggc ataccgacca
     3121 gcctggcgat ggtggatctc gaccacttca agcgcgtcaa cgacgactat ggccacgagg
     3181 tcggtaacca ggcgctgcag cacatggccg atatcctgat gtcggaggtc aggacgacgg
     3241 atatcgtctg tcgttacggc ggcgaggaat tcgcgatgat ttttccggag acccacctca
     3301 acctggctct caaggtggcg gatcgtatac gcctggaaat tgccagccgc ccgttgcaga
     3361 ccgagcacgg cgaaatcaag atcaccgcca gcatgggtct cagcgtctac atgaagacca
     3421 gcattctcga catcgacgac ttcatcgagt cggtcgacaa atacctctac gaggccaagc
     3481 aatcggggcg caactgcatt tgccacgtcg attacaacga cctgcgcaag gtcaccgaga
     3541 tcggctccgt cgagcgcgcg ctgctcttcg acgacaaaat ctaggcgttt cgttccccca
     3601 aaacggcaac cgaagctgtc agtcgagact agccgggtgg cctgcggctt cccgaaaaat
     3661 ggcatttttt cgggggcaag gcgcgattcg acttccatgt ctcagcgcgc cacgggcgac
     3721 atccatgtcg cccgacccct gaaaaaatac catttttggg cacccggcct ttaggtcgcg
     3781 actgacagct ttggttgcct cataagtact tcgatatctg caaccgaagc ctcgattctc
     3841 ataccgggaa ctttcaggtg cggacgccgg gaagccagtc agccgttttc ggccttgtag
     3901 cgctcgatcg attcgaggat ttcgcgcttg gcttcctcgg cctcgaccca gccgtcgatc
     3961 ttcacccact tgccgggctc gagatccttg taatggtcga agaagtgcgc gatcgagtcg
     4021 agcagagccc tcggcaggtc gcgtaccgtg tccatgtggg tgtacagtcc ggacagcttg
     4081 tcgatcggca cggcgacgat cttcgcgtcc ttgccggcct cgtcggtcat gcacagcatg
     4141 cccagcgggc gcacgcggat caccgcgccg ctgatcaccg ggatcggcgt caccaccagc
     4201 acgtcgaccg gatcgccatc ctcggacagg gtttgtggca cataaccgta gttacacgga
     4261 tagtgcatcg ccgtgcccat gaagcggtcg acgaacatcg cgccggtgtc cttgtcgacc
     4321 tcgtacttga cggggtcgct gtgcgacggg atctcgatga tgacgttgat gtcattgggc
     4381 acgttcttac cgctgctgac ccggtccaga atcatgatgc tgtccactcg agtttcggga
     4441 tcgaaagtat aaccaagcgg cgaatattaa tcagcggccc ggcggtcgcc ggaaaaattt
     4501 caatcctgac tatcagagtt tgaactcggc cagttttttc tctaccgcct ggttcttcag
     4561 caccacgtag cgcggcatgc cgcgctcgtc gtagtcggga tagtcttcgc cctggatcag
     4621 cggatagagg tactcgcggc actgcgcggt gatgccgaag ccgtcgtcgg taatgaaatc
     4681 gcgcggcatg ggtttctcga cattggcgac ctcggcgagc ggcgcgcgcc cgattcgcca
     4741 gctatagggg ttcgatgaaa tccgctcgat agtcggcatg atcgcgtttt cgccgtcgag
     4801 cgccagctcg acactgacct tgccgagttc ataggcctgc tcgacatcgg ttgcggaagc
     4861 caggtggcgc gcggcccgct gcaggtaatc ggccaccgcc cagtgaaact tgtaaccgag
     4921 ggcctccctg actatttcgg caaccaccgg cgcgacgccg ccgagttgcg catggccgaa
     4981 cgcgtcgcgg gtgccctgct cggcgagaaa gctgccgtcg gcattgcgcg caccctcgga
     5041 gaccaccacc gtgcagtagc cgtgccgttc gacattctgt ttcaccgcgg cgatgaaacg
     5101 ctcgcgatcg aagtcgatct cggggaacag gaccagcacc gggacgccat agtcctcgat
     5161 caggccgccg gccgcggcga tccagccggc atgacggccc atgacttcga gaatgaagac
     5221 cttggtcgag gttttcgcca ttgatgcaac atcgaaggaa gcctcgagcg cggataccgc
     5281 gatgtacttg gccaccgagc cgaacccggg gcaattatcg gtgatcggca ggtcgttatc
     5341 gaccgtcttc ggcacgtgta tggcctgcac cgggaaaccc atggtctccg acagttgcga
     5401 taccttgtaa caggtatccg ccgaatcgcc gccgccgttg taaaaaaagt agccgatgtc
     5461 gtgggcgcgg aatacctcga tcaggcgttc gtactcgcgg cggttttcct ccagtccctt
     5521 gagcttgaag cggcaggagc cgaacgcgcc cgacggggtt ttcctgagcg cggcgatgtc
     5581 ggcatcgctc tctttcgagg tatcgatcaa atcctcggtc agcgcgccga tgataccgtt
     5641 gcgcccggca agcacgttgc cgatacggtc gccgtgcccg cggcaggttt cgatgacgcc
     5701 gcaggcgctg gcattgatga ccgaggtgac gccgccggat tgcgcgtaaa aggcattttt
     5761 cttactcatg gctggaatcg cgtggttttc atggttgact gagaataccg caaaaaaatc
     5821 cgaaaaacag tcagccgcgg caataaaaaa ggggtagcgc gtgcgctacc ccctgcagga
     5881 cctgtcgacc tgtcagccga gcagcggtgc aatgaccagg ctgacgatcg ccatgacgtt
     5941 gatgagaatg ttcatcgacg ggccggaggt atccttgaac ggatcgccga cggtgtcgcc
     6001 gacgacggcg gccttgtggg cgtcggtgcc cttgccgccg tgcgcgcccg actcgatcgt
     6061 cttcttggcg ttgtcccagg cgccgccggc attggccatc atcagtgcca tcagcacgca
     6121 ggacagcagc gcgccagcca gcatgccgcc gagcgcctcg gggccgatca ggaagccgac
     6181 cagcggtggc gcgctgaccg ccaccgtgcc ggggatgatc atcttcttca atgccgcggt
     6241 ggtcgcgata tcgacgcatt gcgcggtatc cggttcggct ttgccctcga gcaagccgtc
     6301 gatttcccga aactggcggc gaatttcctt gatcatttca aaggccgcgt cgccgaccgc
     6361 ggtcatcgtg atcgatgcga tcacgaacgg gatgacaccg ccgatgaaga cgccgatcag
     6421 cacggtcggg ctattcagca ccagttcgaa attctcgatg ttcagcgaca cggtttcgat
     6481 ataggcggtg atgatcgcca gcgcggcgag cgcggcggcg ccgatcgcga aacccttgcc
     6541 gatcgcggcc gtggtgttgc ccagttcgtc gagcgaatcg gtgatcttgc gggtgtcggc
     6601 gccgagaccg gccatctccg cgataccgcc ggcgttgtcg gcgaccgggc cgtaggcgtc
     6661 gatcgccatg gtgatgccga ccgtggccag catgccgacc gccgcgatac cgaccccgta
     6721 gaggccggcg aaatgactcg agatccagat gatgccgcaa atcgtcagga tcggtatggc
     6781 gaccgattgc atgccgatcg caagcccgct gatcatcacc gtggccgggc cggtctcgcc
     6841 ggacttggcg atgtgctcga ccggtttgcc cgccgtgtag tactcggtga tcaggccgat
     6901 gacgatgccg ccgacggcac ccgccagtac cgctacccag ataccgctga ccagacccag
     6961 cgcccagatc aggaagtaag ccagcgcgat aaacaggatc gacgagccga tggtgccggt
     7021 acgcagcgcc ttttcaggcg actttgccga attcatcttg acgatcagga taccggcgat
     7081 cgagcacagc agtcccagcg acgccagcgc cagcggcagg aacatcatgt tctcctgacc
     7141 gccgaattcg tcggccaccg ccagcgtcat ggtcgaggcg atcgcgatcg acgcgatcat
     7201 cgaaccgcag taggattcga aaatatccga acccatgccg gcgacgtcgc cgacgttgtc
     7261 gccgacgttg tcggcgatga cgcccggatt acgcgggtcg tcctcgggaa tgccggcttc
     7321 gaccttgccc acgaggtc
//
LOCUS       DATCZV010000572         7135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_310785, whole genome shotgun sequence.
ACCESSION   DATCZV010000572 DATCZV010000000
VERSION     DATCZV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310785"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VIW27_12955"
     CDS             <1..209
                     /locus_tag="VIW27_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740629.1"
                     /translation="RHGNLATTGMDAGSRAGMTGNSRQRGRRDHCGPEPQSIHGIAEG
                     RPLRIVPGRATIAGRIGMPARGPA"
     gene            complement(256..1431)
                     /locus_tag="VIW27_12960"
     CDS             complement(256..1431)
                     /locus_tag="VIW27_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY5740630.1"
                     /translation="MSSTVKLFQPLTIRATTLPNRIVVSPLCQYSAIDGMAQPWHFAH
                     LSTFARGKSGLVFTEATAVEARGRITPECLGIWNDAQAEALRPIVEFIESQGCVPGIQ
                     LAHAGRKASTCSPFSEWGPVPLVKADSERAGEPWQTVAPSPLPVADGWHQPAELDTAG
                     LEQAKQAFVDAAVRACETGFRVLELHMAHGYLLHSFLSPLGNQRGDAYGGDLDGRMRF
                     PLEVSAAVRAAVPDGVPLFARISAIDGREGGWTLDDSVQLARKLAELGVDVVDCSSGG
                     IGGPPRFRSGDDGKPLTRDSARVPGFQVPFSRAIREQVDIKTMAVGVIIDPHQAEEIL
                     QDGGADLVALGRELMWDPFWPLRAAETLGVDTDYRMWPPQYAWAVDRRAQIKALNES"
     gene            complement(1550..2779)
                     /locus_tag="VIW27_12965"
     CDS             complement(1550..2779)
                     /locus_tag="VIW27_12965"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5740631.1"
                     /translation="MSDANIFSPDFNGLSAAQPTLPYRYYLDDQQYRRELDTIWSRNW
                     IYLCRAETLANSGDYRLFEIAGRELILLRDDAGGLAAYFNSCRHRGSTLCRDAAGRFE
                     RGRITCPYHSWMYDLRGRLLATGGARPVDGFAREEHGLDAVAIAEWGGFVFANLEGGD
                     APAFAAALGDELAPLANWPLAELRVGHRFSKTLDCNWKLFWENFNECLHCPKVHPGLC
                     QLVPIYGRGLMVRKDDPRWRERGEDDNPRISGRLRAGAETWSSDGRAHAVVFRGLSDA
                     ERQRGHTYAVALPSLFIVGHVDYVRSVQITPLGSEKMRIDAEWLLTPEALEDAALDLP
                     ALAGFAEQVMREDGEICEINHRGLRSAATPHGVLMQEEYEVFAFQEWIRRELGEAPLA
                     AARASRASRRKESDSND"
     gene            complement(2820..4283)
                     /locus_tag="VIW27_12970"
     CDS             complement(2820..4283)
                     /locus_tag="VIW27_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY5740632.1"
                     /translation="MTEERAETGRLQDLLTQIGSGLDEYLKFEYPDALAARDFWQAAL
                     DRPVPQQGIGIDAVVDEINTFLVPYGPPIPRPGFTGYIVTGGSTASALASTAAHIASP
                     VRYLPTAFNFVEELSLDWLAQMFGLGAMKGIYSSGGSVANLIGLGAARQAAFEAIGHD
                     PARDGIDRPVGVYASSECHHTIQRSCGVLGVGRRALRPIACDDAGRLRVDALQAAIDA
                     DLGNGILPMAIVANAGTTSCGAIDPLAEIAALARRRGIWLHVDGAYGLPGVLDSRVSH
                     LYQGIELADSVIIDPHKWLGAAAGVAATFVHDRGLLERAFTQERSDYLEGSIEQQDSL
                     QPRVEHSMDDFSIPYYDYGVELTAPCRGIVVWAMLREIGVEGMRARILRHNDMAREVA
                     AHCRRHDNLELLLEPTLSICCFRYVAPGVADLDLLNQRLHRRLIRENEFMPSTTRVDG
                     RLALRPCFLGARTERDQVDGMLQSILRIGAALAAEMQ"
     gene            complement(4345..6411)
                     /locus_tag="VIW27_12975"
     CDS             complement(4345..6411)
                     /locus_tag="VIW27_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5740633.1"
                     /translation="MTQRRLIVSLLVFFVALALPSSILVYQAYGQLKWEAFYQHQRLA
                     RELTLRIDSDFRDLIAREEKRPISDYEFLNVEGSGDSAFLQRSPLSEFPPPAEVPGLL
                     GYFQVDASGQLRTPLVPETNAGDYGITAAELAARRQQEERIRGILDRNRLVTRSDERL
                     ASALDDGIAEISQAPLSMAPEFADAPPDADGQKASPAKLKSERLGQAAFDALSESRPR
                     MDDSSGTPASQVGELALEDRYDVAAGAGAATTQAELEQQAGARRLRKEQVNLPRSVAA
                     DAPLPRALPSMDEEEATGLSRGRPEIRIQTFESEVEPMEFALLDSGHFVLFRRVWRDD
                     ERQVQGLLIEQAQFVERLVAPAFRESSLSSMSRLIVAWQGTILRSFEPDYGRLYSSAA
                     GRESRELLYQGRLVAPFGDLELIYTLAYLPVGAGGQVIIWSAVVLALVLVGGCLMLWR
                     LGRRQLALARQQQDFVSAVSHELKTPLTSIRMYGEILREGWADENKRKAYYDFIFHEA
                     ERLTRLINNVLQLARLSRNEQPASLSATRVDAALDELRPRLESQLEAAGFELAIDIDA
                     AAAESKIEIDMDGFLQIFINLVDNAVKFSANAERQKIDIACRRLRDGKIQFAVRDCGP
                     GIAPDQMKKIFTLFYRSENELTRETVGTGIGLALVQQLAGGMHAEVDVVNCEPGAEFR
                     LRFPAC"
     gene            complement(6408..7103)
                     /locus_tag="VIW27_12980"
     CDS             complement(6408..7103)
                     /locus_tag="VIW27_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5740634.1"
                     /translation="MAQKARLLIVEDEAAIRSGLVDVFVYHGYEVETADNGDDGLRLA
                     LGGNYDLVLLDVMLPGVDGFEICNRIRQQDRQQPIIMLTAKSSDEDIVQGLTLGADDY
                     VAKPFSVQQLVLRVQAVLRRSGLGLAAAREISLDNGLVIDTENLCAGETKFTRREMDV
                     LQYLARQSDRPVAREELLARVWGYANHLEIETRTVDIHIAKLRRKIEADPSNPQSLVT
                     VRGAGYRLILADA"
BASE COUNT         1243 a         2389 c         2315 g         1188 t
ORIGIN      
        1 cccggcatgg gaacctcgcc actaccggca tggatgccgg ctcgagggcc ggcatgacgg
       61 ggaattcgcg gcaacgcggc cggcgcgatc attgcggccc cgagccgcaa tccattcacg
      121 gcatcgccga gggccgcccg ctgcggatcg tcccgggacg ggcaactatt gccgggcgca
      181 ttgggatgcc ggctcgaggg ccggcatgac gagatctaga gttgtagccg gtgcggctat
      241 taccgcctac ccggatcagg attcgttgag agccttgatc tgcgcgcggc ggtcgacggc
      301 ccaggcatac tgcggcggcc acatacgata atcggtatcg acgccgagtg tttcggccgc
      361 gcgcagcggc cagaacgggt cccacatcag ttcacggccg agcgcgacca ggtcggcacc
      421 accgtcctgc aggatttcct cggcctggtg cggatcgatg atcacaccaa ccgccatcgt
      481 cttgatatcg acctgttcgc gaatcgcgcg cgagaaaggc acctggaagc caggcacgcg
      541 cgccgagtcg cgggtcagcg gcttgccgtc atcaccactg cgaaagcgcg gcggcccgcc
      601 gatgccgccc gacgaacagt cgacgacatc gacaccgagc tcggccagct tgcgcgccag
      661 ctgcaccgag tcgtccagcg tccagccgcc ctcgcggcca tcgatcgccg agatgcgcgc
      721 gaacaacggc acgccgtcag gaaccgcggc gcgcaccgct gcgctcactt ccagcggaaa
      781 ccgcatgcgg ccgtcgaggt cgccgccgta ggcgtcgccg cgctgattgc cgagcggcga
      841 cagaaagctg tgcagcaggt agccgtgcgc catgtgcagc tcgagcacgc gaaaaccggt
      901 ctcgcaggcg cgcaccgcgg cgtcgacgaa tgcctgtttg gcctgctcca ggccggcggt
      961 gtcgagctcg gccggctggt gccagccatc ggcaaccggt agcggcgacg gcgccacggt
     1021 ttgccagggc tcgccagcac gttcgctgtc cgccttgacc agtggcaccg gcccccactc
     1081 ggaaaacggc gagcaggtcg aagccttgcg cccggcgtgg gccagctgga tgccgggaac
     1141 gcaaccctgc gattcgatga actcgacaat cggccgcagc gcctcggcct gcgcgtcgtt
     1201 ccagataccg aggcactccg gcgtgatgcg accacgcgcc tcgaccgcgg tggcctcggt
     1261 gaacaccagc ccggacttgc cgcgcgcgaa ggtactgaga tgcgcgaaat gccagggttg
     1321 ggccatgccg tcgatcgccg agtactggca cagtggtgag acgacgatgc gattcggcaa
     1381 ggtggttgcg cggatggtaa ggggctggaa aagttttact gtgctggaca tggcataaac
     1441 acggcggacg gcgaaaaggg cgcaattatc gggcttcacg cggcaaatat caaaacaccc
     1501 ggctgcggcg agcacggccg gggcctgccg catccggtac tgccgaacct cagtcgttcg
     1561 aatcgctttc ctttctgcgg ctggcgcggc tggcgcgggc tgccgcgagc ggcgcttcgc
     1621 cgagctcgcg gcgaatccat tcctggaacg cgaatacctc gtattcctcc tgcatcagca
     1681 cgccgtgcgg cgtcgccgcc gagcgcaggc cgcgatgatt gatctcgcaa atctcgccgt
     1741 cctcgcgcat cacctgttcg gcgaagccgg ccagcgcggg caggtccagc gcggcatcct
     1801 cgagcgcctc gggggttagc agccactcgg cgtcgatgcg catcttctcg ctgccgagcg
     1861 gcgtgatctg taccgagcgc acgtagtcga catgaccgac gatgaacagc gacggcagcg
     1921 ccacggcata ggtgtgcccg cgttgccgct cggcgtcgct cagcccgcga aacacgaccg
     1981 cgtgcgcgcg cccgtccgac gaccaggtct cggcgccggc acgcagccgg ccgctgatcc
     2041 gcgggttgtc atcctcgccg cgctcgcgcc accgcggatc gtccttgcgc accatcaacc
     2101 cgcggccgta gatcggcacc agctggcaca ggccgggatg caccttcggg caatgcaggc
     2161 attcgttgaa attttcccag aacagtttcc agttgcaatc gagcgtcttg ctgaaacggt
     2221 ggccgacgcg cagctcggcc agcggccagt tggcgagcgg cgccagctca tcgccgagcg
     2281 cggccgcgaa tgccggcgcg tcgccaccct ccaggttggc gaacacgaag ccgccccact
     2341 cggcgatcgc gaccgcgtcc aggccgtgtt cctcgcgcgc gaagccatcg accgggcgcg
     2401 cgccgccggt ggccaacagt cgcccccgca gatcgtacat ccagctgtgg taagggcagg
     2461 tgatgcgccc gcgttcgaaa cggcccgccg cgtcccggca cagcgtcgac ccccgatgcc
     2521 ggcaactgtt gaagtaagcc gcgagaccgc cggcgtcgtc gcgcagcagg ataagctcgc
     2581 gcccggcgat ttcgaacagg cggtagtcac cgctgttcgc cagggtttcc gcgcggcaca
     2641 ggtagatcca gttgcgtgac cagatggtat cgagttcgcg gcgatactgc tggtcgtcga
     2701 gataatatcg atacggcagc gtcggctggg ccgcgctcag gccgttgaag tcggggctga
     2761 aaatattcgc atcgctcatc gcggttcgct ccaaacggcg ccggccggga gcgaaaattt
     2821 tattgcattt cggcggccag cgcagcgcct attctgagga tcgactgcag catgccatcg
     2881 acctggtcgc gctcggtacg cgcgccgaga aaacacggcc gcagcgccag ccgcccatcg
     2941 acgcgcgtag tgctgggcat gaattcgttt tcgcgaatca ggcgccggtg cagccgctgg
     3001 ttgagcaggt cgagatcggc gaccccgggc gccacgtagc gaaaacagca gatcgacagc
     3061 gtcggttcga gcagcagctc gaggttgtca tgacgccggc aatgcgcggc aacctcgcgc
     3121 gccatgtcgt tgtgccgcaa aatccgcgcg cgcatgccct cgacgccgat ctcgcgcagc
     3181 atcgcccaga cgacgatacc gcggcagggc gcggtcagtt cgacgccgta gtcgtagtat
     3241 ggaatgctga aatcgtccat cgagtgctcg acccttggct gcagcgaatc ctgttgttcg
     3301 atgctgccct ccaggtagtc ggagcgctcc tgcgtaaacg cacgctccag cagtccgcga
     3361 tcgtgtacga aggtggccgc caccccggct gcggcgccca gccacttgtg tggatcgatg
     3421 atcaccgagt cggcgagctc gataccctgg taaaggtggc tgacgcggga atcgagcaca
     3481 ccgggcagac cgtaggcgcc gtcgacgtgc agccagatgc cgcgacgccg cgccagcgcc
     3541 gcgatctcgg ccagcggatc gatggcaccg caactggtgg tgccggcgtt ggcgacgatc
     3601 gccatcggca gaatgccgtt gccgagatcg gcatcgatcg ccgcctgcag cgcatcgaca
     3661 cgcaggcgcc cggcatcatc gcaggcgatc ggccgcagcg cgcgccggcc aacgccgagc
     3721 acgccgcagg aacgctgaat ggtatggtgg cattcgctgc tggcgtagac gccgaccggg
     3781 cggtcgatgc cgtcgcgagc cggatcgtga ccgatcgcct cgaacgccgc ctggcgcgcc
     3841 gcgccgagcc cgatcaggtt ggccaccgaa ccgccgctgg agtagatgcc cttcatcgcg
     3901 ccgaggccga acatctgtgc cagccagtcg agcgacaact cctcgacgaa attgaacgcc
     3961 gtcggcaggt agcgcaccgg cgaggcgatg tgcgcggccg tgctggcgag ggccgacgcg
     4021 gtgctgccgc cggtaacgat gtagccggtg aatcccgggc gcgggatcgg cgggccgtag
     4081 ggcaccagga aagtattgat ctcgtcgacg accgcgtcga taccgattcc ctgctgcggc
     4141 accgggcggt cgagagcggc ttgccagaaa tcgcgcgctg cgagcgcgtc gggatactcg
     4201 aacttcaggt actcgtccag cccgctgccg atctgcgtca acaagtcttg cagccgaccg
     4261 gtctcggctc tctcttctgt cattggtcta cgctgctcta tgtttggcga tcaggcgcga
     4321 acgatagcat gaaaaaccgg cttgttaaca agccggaaaa cgcagccgga actcggcccc
     4381 cggctcgcag ttgacgacgt ccacttcggc atgcatgccg ccggcgagct gctgtaccag
     4441 cgccagaccg atgccggtgc cgacggtttc acgcgtcagt tcgttttcgc tgcggtagaa
     4501 cagcgtgaag atttttttca tctggtcggg cgcgatgccc gggccgcagt cgcgcaccgc
     4561 aaactgtatc ttaccgtcgc gcaggcgccg gcaggcgatg tcgattttct gccgttcggc
     4621 attggccgaa aacttgaccg cgttatcgac caggttaatg aaaatctgca gaaagccgtc
     4681 catgtcgatc tcgatcttcg attccgcggc ggcggcatcg atatcgatcg ccagctcgaa
     4741 gccggcagcc tcgagttgcg attcgagccg cgggcgcagt tcgtcgagtg cggcatcgac
     4801 gcgggttgcc gacaggctcg ccggctgttc gttgcgcgac aggcgcgcca gctgcaagac
     4861 gttgttgatc aaccgcgtca ggcgttcggc ctcatggaaa ataaaatcgt agtaagcctt
     4921 gcgcttgttc tcgtcggccc agccctcgcg caggatttcg ccgtacatgc gaatcgaggt
     4981 cagcggtgtc ttcagctcgt ggctgaccgc ggagacgaaa tcctgttgct ggcgcgccag
     5041 tgccagctgc cgccggccga ggcgccagag cattaagcag ccgccgacca gtaccagtgc
     5101 caacaccact gccgaccaga tgatcacctg gccgccggcg ccgaccggca ggtaggccag
     5161 cgtgtagatc aattcgaggt cgccgaacgg tgccaccagc cgcccctggt agagcagctc
     5221 ccggctttcg cgccccgccg cggagctgta aagccgcccg taatcgggtt cgaagctgcg
     5281 caggatcgtg ccctgccagg cgacgatcag gcggctcatc gacgacaggc tggattcgcg
     5341 gaacgccggc gccaccagcc gctcgacgaa ttgcgcctgc tcgatcagca ggccctgcac
     5401 ctggcgctcg tcgtcgcgcc agacgcgtcg aaagagcacg aaatgaccgc tgtcgagcag
     5461 cgcgaactcc atcggctcga cctcgctttc aaaggtctga atgcgaatct ctggtcgacc
     5521 gcgcgacagc ccggtcgcct cctcctcgtc catcgatggc aaggcccgcg gcagcggcgc
     5581 atcggcagca acgcttcgtg gcaggttgac ctgttccttg cgcagccgcc tcgcgccagc
     5641 ctgttgctcg agttcggcct gcgtcgttgc cgcgccagcg ccggcggcga cgtcgtagcg
     5701 atcctcgaga gcgagttccc cgacctggct ggccggcgta cccgacgagt cgtccattcg
     5761 cggccggctc tcgctgagcg catcgaaggc cgcctggccg agacgttcgg atttgagctt
     5821 cgccggagaa gccttctggc cgtcggcgtc cggcggcgcg tcggcgaatt cgggcgccat
     5881 cgacagcggc gcttgcgaga tctccgcgat gccatcgtcg agcgccgacg cgagccgctc
     5941 gtcacttctg gtcaccagcc ggttgcggtc aagtatgccg cgaatgcgct cttcctgttg
     6001 ccggcgcgcg gcgagctcgg ccgccgtaat accgtaatcc ccggcattgg tctccggcac
     6061 cagcggcgtg cgcaactgcc cggacgcatc gacctggaag taaccgagca gccccggcac
     6121 ctccgccggc ggcggaaact cggacaacgg cgagcgctgc aaaaaggcgc tgtcgccgct
     6181 gccctcgaca ttgagaaatt cgtagtcgct gattggacgt ttttcctcgc gcgcgatcag
     6241 atcgcggaaa tcgctgtcga tgcgcagcgt caactcgcgc gccagccgct ggtgctggta
     6301 aaaggcttcc catttcaatt gcccataggc ctggtagacg aggatcgaac tcggcagcgc
     6361 cagcgcgacg aaaaacacca gcagcgagac gatgagccgc cgctgggtca tgcgtcggca
     6421 agtatcagcc ggtaaccggc gccgcgcacg gtgaccaggc tttgcgggtt cgacgggtcg
     6481 gcctcgatct tgcgccgcag cttggcaata tgaatgtcga cggtgcgggt ttcgatttcg
     6541 agatgattgg catagcccca gacccgcgcc agcaattcct cgcgggccac cgggcggtcg
     6601 gattgccgtg ccaggtattg cagcacatcc atttcgcggc gggtgaattt ggtttcgccg
     6661 gcacacagat tttcggtatc gatgaccagg ccattatcca ggctgatctc gcgcgccgcg
     6721 gccagcccga ggccggagcg gcgcaacacc gcctgcacgc gcaataccag ttgctgcacc
     6781 gagaacggct tggcgacgta atcgtcggca ccgagtgtca ggccctgcac gatatcttcg
     6841 tcgctcgact tggcggtcag catgatgatc ggctgctgcc ggtcctgctg gcgaatacgg
     6901 ttgcagattt cgaagccgtc gacacccggc agcatgacgt cgagcagcac cagatcgtag
     6961 ttgccgccga gcgcgagccg cagaccgtcg tcaccgttgt cggcggtttc gacctcgtag
     7021 ccgtggtaga cgaacacgtc gaccagaccg ctgcgaatcg cggcctcgtc ctcgacaatc
     7081 aacaagcgcg ccttttgcgc catcgcttcg atccctggtt aatcacgctt caatc
//
LOCUS       DATCZV010000573         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_99021, whole genome shotgun sequence.
ACCESSION   DATCZV010000573 DATCZV010000000
VERSION     DATCZV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99021"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="uvrB"
                     /locus_tag="VIW27_12985"
     CDS             complement(<1..1422)
                     /gene="uvrB"
                     /locus_tag="VIW27_12985"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY5740635.1"
                     /translation="MARQFELETEYQPAGDQPQAIDALVQGISDGLMHQTLLGVTGSG
                     KTFTVASLIQRLQRPAIIMAHNKTLAAQLYGEMKEFFPRNSVQYFVSYYDYYQPEAYV
                     PASDTFIEKDASINEHIEQMRLSATKSLMERRDTVIVASVSAIYGLGDPDSYLKMLLH
                     LVVGDEIDQRSILRRLAEIQYTRNDIELKRGTYRVRGEVIDVHPADSEREAVRIELFD
                     TEVEKISLFDPLTGEVSRDVKRITIYPKTHYATPREVILASIDEIKEELKERLAELNA
                     ADKLVEAQRLEQRVNYDIEMMRELGYCSGIENYSRFLSGRRPGEPPPTLFDYLPDDCL
                     LFIDESHVSVSQIGGMYRGDRSRKETLVEYGFRLPSALDNRPLRFDEFERMSPQTVFV
                     SATPGDYEKKYSGAVVEQVVRPTGLVDPLVEVRPASSQVDDVLSEIHKRVERQERVLI
                     TTLTKRMAEDLTDYLDEHGVRVRY"
     gene            1525..>2051
                     /locus_tag="VIW27_12990"
     CDS             1525..>2051
                     /locus_tag="VIW27_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019593142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5740636.1"
                     /translation="MFNDLSKRVRAIKPSPTLAVSNLASQLRAEGRDVIGLGAGEPDF
                     DTPDHIREAAIEAIRGGDTHYTAVDGTPALKQAIVDKFERDNGLSYGADQILVSCGGK
                     QSFFNLCQALLDAGDEVVIPAPYWVSYPDIVILADATPVIVAADIDSGFKITAAQLEA
                     AITPQTRMLVLNSPS"
BASE COUNT          372 a          665 c          618 g          396 t
ORIGIN      
        1 gtaacgcacg cgcacgccgt gctcgtcgag ataatcggtc agatcctcgg ccatgcgctt
       61 ggtcagcgtg gtaatcagca cgcgttcctg ccgctcgacg cgcttgtgga tctccgacag
      121 cacgtcgtcg acctggctcg acgccgggcg cacctcgacc agcgggtcga ccagtccggt
      181 cggtcgcacc acctgctcga ccaccgcgcc gctgtacttt ttttcgtaat cgcccggggt
      241 cgccgagacg aacacagtct gcggcgacat gcgctcgaac tcgtcgaagc gcagcggccg
      301 gttgtcgagc gccgacggca ggcgaaagcc gtactcgacc agggtttcct tgcgcgaacg
      361 gtcgccgcgg tacatgccgc cgatctgcga gaccgagaca tggctctcgt cgatgaacag
      421 caggcagtcg tccggcaggt agtcgaatag cgtcggcggc ggctctcccg gccggcgccc
      481 ggataaaaac cgcgagtaat tctcgattcc cgagcaatag ccgagttcgc gcatcatttc
      541 gatgtcgtaa ttgacccgct gctccaggcg ctgtgcctcg accagcttgt cggccgcgtt
      601 gagttctgcg agccgctcct tcaactcttc cttgatctcg tcgatcgacg ccaggatcac
      661 ttcgcgaggc gtcgcgtagt gcgtcttcgg gtaaatagtg atgcgcttga cgtcgcggct
      721 gacctcgccg gtcaacgggt cgaacaggct gatcttctcg acctcggtgt cgaacagttc
      781 gatgcgcact gcctcgcgct cggaatcggc cgggtgcacg tcgatgacct cgccgcgcac
      841 gcggtaggtg ccgcgcttca gctcgatgtc gttgcgcgtg tactggatct cggccagccg
      901 ccgcagaata ctgcgctggt cgatctcgtc gccgaccacc aggtgcagca gcatcttgag
      961 gtaggaatcc gggtcgccga ggccgtagat cgccgacacc gaagcgacga tcaccgtgtc
     1021 acggcgctcc atcagcgact tggtcgccga cagccgcatc tgctcgatgt gctcgttgat
     1081 cgacgcatcc ttctcgatga aggtatccga ggccggcacg taggcttccg gctggtaata
     1141 atcgtagtag gagacgaagt actgcaccga gttgcgcggg aagaattcct tcatctcgcc
     1201 gtagagctga gccgccagcg tcttgttgtg cgccatgatg atcgccgggc gctgcagccg
     1261 ctggatcagg ctggctaccg tgaaggtctt gcccgagccg gtgaccccga gcagcgtctg
     1321 gtgcatgagc ccgtcgctga tgccctgcac cagcgcatcg atcgcctgcg gctggtcgcc
     1381 ggcgggctga tactcggtct cgagctcgaa ttgccttgcc ataaaaaaat ttatcgtgaa
     1441 gattaataca ggtcgcttta tcggcgccaa agggccacgt atactgcgcg gtttcagcag
     1501 ccaccgccaa cagggtcagc agccgtgttc aacgatttgt cgaagcgcgt acgcgcgatc
     1561 aaaccatccc ccactttagc agtttcgaat ctggcgagcc agcttcgcgc agagggccgc
     1621 gacgtcatcg gcctcggcgc cggcgagccc gatttcgaca ccccggatca tatccgcgag
     1681 gcggcaatcg aggcgattcg cggcggcgat acccactaca ccgcggtcga cggcacgccg
     1741 gcattgaaac aggccatcgt cgacaagttc gaacgcgaca acggtctcag ttacggcgcc
     1801 gaccagatcc tggtgtcatg tggcggcaag cagagctttt tcaacctctg ccaggcactg
     1861 ctcgacgccg gcgacgaagt cgtcattccg gcgccctact gggtgtctta tccggatatc
     1921 gtgattctcg ccgacgccac gccggtgatc gtcgccgccg atatcgacag cggcttcaag
     1981 ataaccgctg cgcagctcga ggccgcaatc acgccgcaaa cccgcatgct ggtactcaac
     2041 agcccgtcga a
//
LOCUS       DATCZV010000574         5138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_682036, whole genome shotgun sequence.
ACCESSION   DATCZV010000574 DATCZV010000000
VERSION     DATCZV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682036"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1493
                     /locus_tag="VIW27_12995"
     CDS             102..1493
                     /locus_tag="VIW27_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5740637.1"
                     /translation="MSAIHYWSACRQAGAIRAGELSSRDLLEACLERIDTVNPAINAV
                     VQLVTERARAEADERDREAAAGRFRGPLHGLPLTVKDSLDTAGIVSTGGTLGRKDHVP
                     DEDAPVVARLRAAGAVLLGKTNTPELTFSAETSNLIYGRSNNPYDLERSPGGSSGASA
                     AIVACGGAALELGSDTGGSIREPAHLCGVAGIKPTSGRTPRSGHIVPHGGGVLDSLTQ
                     IGPLARHVEDLALALPLICGPDGRDPAVVPVPVADPAAVDLKSLRIAWHDDNGIVTPA
                     DDIRRVVAETASALRKAGFAVESRLLPETRLLVDLTSKFRRMTNAGFIMRLLQRYGSE
                     TIGEELDGYLSEESVASANHVDTALLEEIDAARSRALGFFADYDAILSPVSFACARPH
                     RASYTDSFDEWGYIQIFNLLGWPGLAVRAGTDVDGMPIGVQLVAAPWREDIVLALGVA
                     VEKLMGGFRPPPI"
     gene            complement(1519..2097)
                     /locus_tag="VIW27_13000"
                     /pseudo
     CDS             complement(1519..2097)
                     /locus_tag="VIW27_13000"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
     gene            complement(2324..3628)
                     /locus_tag="VIW27_13005"
     CDS             complement(2324..3628)
                     /locus_tag="VIW27_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY5740638.1"
                     /translation="MLQNLRTSTWFMSGILLGLSLAIGHSVYALKDDERQIPFEELQA
                     FTEVFSKVKSDYVETVDDKKLIEDAIRGMLNGLDPHSAYLNLEEFSDLKIGTTGQFGG
                     LGIEVGMENGFVKVISPIDDTPASRAGIQASDLIIKLDDKSVKGMTLNEAVNIMRGKP
                     NTDISLTIVREGEPKPLVIKITREIIRVKSVKNRMLEPGYGYVRITNFQSRTTTDLLK
                     AVSDLQKQEQLKGMILDLRNNPGGVLNGAVGVSDAFIDEGLIVYTEGRVDDSSHRYLA
                     TPGDSLNGAPLVVLINGGSASASEIVAGAMQDHKRGIIMGTKSFGKGSVQTIQELRNG
                     SAVKLTTARYFTPSGRSIQARGIEPDIVLSTVKLSANDEQARATYSESDLEGSLSNPD
                     GDPADGDKDDDTRDELAKTDFQLYEALNLLKGLNILNGIGQG"
     gene            complement(3815..4990)
                     /locus_tag="VIW27_13010"
     CDS             complement(3815..4990)
                     /locus_tag="VIW27_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5740639.1"
                     /translation="MSISRHSLWPVIALLILAVTIAGRADERSAKESQLREVLQQIEQ
                     LKQAIEVKENSKSEYIQQLKSVETQIGSINRKIRRIRQQIDAGKTRLSALRETRRQHQ
                     RQLSTETEYLAEQVYAAFTMGRQERVKMLFSQRDPERLQRNLVYYQYFSNARVALIDD
                     VQENIERLIETEARIEEARRQLEADRADLDAQKQRLAADRDQRESIIASLDRQLAQQG
                     GSLSRLEQEARQLQQLIDSIQQLLDEAPEGEISRARFAELKGRLAWPVEGKVRRLFNR
                     KKPYSDLRWQGVVIEAPGGREVRAVSHGRVAFADWLRGLGNLIIIDHGNSYLSLYGHN
                     ESLYKSAGEWVDAGNVIASIGSSGGQQEAGLYFEIRKNGKPQNPTRWCKSGNGFSPG"
BASE COUNT          882 a         1557 c         1645 g         1054 t
ORIGIN      
        1 gctggcatga tcggcccggc agccgttgac ggaccggcag gctcacggtc ggtcaccaaa
       61 ttctggtgca atattgcgcc tggcgaagga taattcgcac catgtccgct attcattact
      121 ggtccgcctg tcggcaggcc ggcgcgattc gtgccggcga actcagctcg cgcgacttgc
      181 tcgaagcctg tctcgagcgt atcgacacgg tcaacccggc gatcaatgcc gtggtgcaac
      241 tggttaccga acgcgcccgc gccgaggccg acgagcgcga ccgcgaggcc gctgccggcc
      301 gttttcgagg cccgttgcac ggcctgccgc tgacggtcaa ggattcactc gataccgccg
      361 gcatcgtttc caccggcggt acgctggggc gcaaggatca cgtgccggat gaggatgcgc
      421 cggtggtcgc gcgcctgcgg gccgcgggcg cggtgttgct cggcaagacc aatacccccg
      481 agctcacctt cagcgccgag accagtaacc tgatctacgg ccgcagcaat aatccctacg
      541 acctcgagcg tagccccggt ggcagcagcg gtgcgtcggc ggcgatcgtc gcctgcggcg
      601 gcgcggcgct ggaactggga tcggataccg gtggcagcat tcgcgagccg gcccacctgt
      661 gcggcgttgc cggcatcaag ccgacctcgg gccgaactcc gcgcagcggc cacatcgtgc
      721 cccacggtgg tggagtgctc gactcgctga cgcagatcgg gccgctggcg cgccatgtag
      781 aagacctggc gctcgcgttg ccgctgatct gcgggcccga tggccgcgat ccggcggtgg
      841 tgcccgtgcc ggttgccgac ccggccgccg tcgacctgaa gtcgctgcgc atcgcctggc
      901 acgacgacaa cggcatcgtc accccggccg acgatatacg gcgtgtggtg gccgagaccg
      961 ccagcgcgct gcgcaaggct ggcttcgcgg tcgaatccag gctgctgccc gaaacccgcc
     1021 tgttggtgga cctgacgtcg aagtttcggc gcatgaccaa tgccggtttc atcatgcgcc
     1081 tgttgcagcg ttacggcagc gaaaccatcg gcgaggaact cgacggctat ctgagcgagg
     1141 aaagcgtcgc cagcgccaac cacgtcgata ccgccctgct cgaggagatc gacgccgcgc
     1201 gcagccgcgc gctcggcttc tttgccgact acgacgcgat cctcagcccg gtgtcgtttg
     1261 cctgcgcgcg gccgcaccgc gcctcctaca ccgatagttt cgacgagtgg ggatatatcc
     1321 agatattcaa tctgctcggc tggccgggac tggcggtgcg cgccggcacc gatgtcgacg
     1381 gcatgccgat cggcgtacaa ctggttgccg cgccatggcg tgaagatatc gtgctcgcgc
     1441 tgggcgtggc tgtcgaaaag ctgatgggcg gttttcggcc gccgccaatc taggcaatat
     1501 cgagcgcgcc ggagccggct agcggacgga ggtttccagc agatgtgccc gttcgggacg
     1561 caggccagtg tgccgcgtgc ggcgcagttc gatcagtcgc gataccggca aaaattcgaa
     1621 agcgctggcc gaagcttgca ggtgtttacg cagtacctcg agggtttcgg aatgcgggtg
     1681 gccgatggcg atggccgagc catgttcgcg cgcgcgggtc agccagatgc gaaattgcag
     1741 gtagatcgat tcggcgtggg tctcgttgtc gagaaatacg tcgcgccgga tcgaggccag
     1801 cccggcatcg agcgcctggg cgtgcgcgac gctcaacggc gaggtgcggc tgtcgagaaa
     1861 gtagagccgc gggtcgtaac tgcgaatgct gtccatcacc gggcgcatga agaagtcgaa
     1921 ctcggtcaaa tgactgccca tgtggttgtt cacgccgcga atatggggca ccgaatccag
     1981 caggctgtgg acccgcccgg taagctggtc ctcgtccatg ctctcgtcga gcgcgttggg
     2041 ttcctgcgcg gcgcgcgagt gaatcgattg catcggcata tgcaggatga cttccttacc
     2101 gcgccggtga ggaagtcatc ctgcatatgc cgatgggcgt aggtcgatcc gggcaggatc
     2161 gcgtaggtat gatcgccgtc gagttcgatc gcggccatgc cattgtcgag cgaatagccg
     2221 agatcgtcga taaccagcga cagcaccggg cgttgatctg ccgctgcgct ggcggcaaac
     2281 agcgccagcg cgatcgccaa cgagcgaatc atggagcgcg gggttagccc tggccgatgc
     2341 cgttgaggat gtttaaccct ttcagcaggt tgagcgcctc gtagagctgg aaatcggtct
     2401 ttgccagttc gtctctggtg tcgtcgtcct tgtcgccgtc cgccgggtcg ccgtcagggt
     2461 tgctcaggct gccctcgaga tcactctccg agtaggtcgc ccgcgcctgt tcgtcgtttg
     2521 ccgacagctt gacggtactc aacacgatat ccggctcgat gccccgggcc tggatcgagc
     2581 ggccgctggg cgtgaagtag cgcgccgtag tcaacttgac cgcggagccg ttgcgcagtt
     2641 cctggatggt ttgcaccgaa cccttgccga acgacttggt gcccatgatg atgccgcgct
     2701 tgtggtcctg cattgcgccg gcgacgattt ccgacgccga ggcggaaccg ccgttgatca
     2761 gcaccaccag tggcgcgccg ttcaggctgt cgccgggcgt tgccaggtag cggtggctgg
     2821 aatcgtccac ccggccctcg gtgtagacga tcaggccttc gtcgatgaat gcgtcggaga
     2881 cgccgaccgc gccattgagt acgccgcccg gattattgcg caggtcgagg atcatgccct
     2941 tgagctgctc ctgcttttgc agatcggaga ctgccttcag caggtcggtg gtggtacgcg
     3001 actggaaatt ggtgatacgg acgtagccgt agccgggttc gagcatacgg ttcttgacgc
     3061 tcttgacgcg aatgatttcg cgggtgatct tgatgaccag cggcttgggc tcgccctcgc
     3121 ggacgatggt caggctgata tcggtgttcg gcttgccgcg catgatgttg accgcctcgt
     3181 tgagcgtcat gcctttgacc gatttgtcgt ccagcttgat gatcaggtcg ctcgcctgga
     3241 taccggcgcg cgaggcgggc gtatcgtcga tcggcgagat caccttgacg aagccgtttt
     3301 ccatgccgac ctcgataccc agcccgccga actggccggt ggtgccgatc ttgagatcgc
     3361 tgaattcctc gaggttcagg taggcggagt gcggatcgag gccgttgagc atgccgcgga
     3421 tcgcatcctc gatcagcttc ttgtcgtcga cggtttcgac gtaatcggac ttgaccttgg
     3481 aaaatacctc ggtaaaggcc tgcagttcct cgaacggtat ctgtctttcg tcatccttga
     3541 gcgcgtagac actgtggcct atcgccagcg acagtcccag caggattcca ctcatgaacc
     3601 aggtggaagt tcgcaaattt tgtagcattc cgattctccg aatagtgcgc gtcggcggta
     3661 agcaagacta aatattgtag accagatgag ggccgattag ttgtaaacaa tacaaaaaga
     3721 tcggccctga aaaacccgat tctacggtaa accgcaaatt attgcagatt tgcggcgcaa
     3781 gccgctggcc ggcgggcgtt ttcatggcgg accgtcatcc ggggctgaaa ccgttgccgc
     3841 tcttgcacca gcgggtcggg ttttgcggct tgccgttctt gcgaatctcg aaatacaggc
     3901 ctgcctcctg ctgcccgccg ctgctgccga tgctggcgat gacattgccg gcatcgaccc
     3961 actcaccagc gctcttgtag agcgattcgt tgtgcccgta gagcgacaga taggaatttc
     4021 cgtggtcgat gataatcagg ttgccgaggc cgcgcagcca gtcggcgaac gcgacccggc
     4081 cgtgggacac cgcgcgcacc tcgcggccgc ccggggcctc gatgacgaca ccctgccagc
     4141 gcaggtcgga ataaggcttc ttgcggttga acagccgcct gaccttgcct tccacgggcc
     4201 atgcgagcct gcccttcagc tcggcaaacc gggctcgcga tatttcgcct tccggcgctt
     4261 cgtcgagtaa ctgctgtatc gaatcgatca gctgttgcag ctggcgggct tcctgttcga
     4321 gccgcgacag gctgcctccc tgctgcgcga gttgccggtc cagcgaagcg atgatcgatt
     4381 cgcgctggtc tcgatcggct gccagccgct gcttctgcgc atccaggtcg gcgcggtcgg
     4441 cttcgagttg gcggcgggct tcctcgatgc gcgcctcggt ctcgattagc cgctcgatgt
     4501 tctcctgcac gtcgtcgatc agcgccacgc gggcgttgga aaaatactgg tagtagacca
     4561 gattacgctg caggcgctcg ggatcgcgct gcgaaaacag catcttgacc ctttcctggc
     4621 ggcccattgt aaatgccgcg tagacctgtt cggcgaggta ttcggtttca gtgctcagtt
     4681 gtcgctggtg ttgacggcgg gtttcacgta atgccgagag gcgggtcttg ccggcatcga
     4741 tttgttgcct gatgcggcga attttgcggt tgatgctgcc gatttgcgtc tccaccgact
     4801 tcagctgctg gatgtattcc gatttgctgt tttccttgac ttcgattgcc tgcttgagct
     4861 gctcgatctg ctgcaggacc tccctcagct gactctcctt cgcgctgcgc tcgtcggccc
     4921 gcccggcaat cgtcaccgcc aggatcagca atgcgatgac gggccaaagg ctatgcctcg
     4981 atatggacat tgatcggcga tttatacata atcagtggct aatattaacg tttggtaata
     5041 aagcaggcaa atggaaaccg aagccctgtc ggtgatcgac ggcaaaaaac tttcgaccca
     5101 tgccgaggat atcgagatcg cctcacggtc gctgaagg
//
LOCUS       DATCZV010000575         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_311045, whole genome shotgun sequence.
ACCESSION   DATCZV010000575 DATCZV010000000
VERSION     DATCZV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311045"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VIW27_13015"
     CDS             <1..668
                     /locus_tag="VIW27_13015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5740640.1"
                     /translation="ANEVLDAMPVKRVVQRERWRELGVGLDGGRFQWRELDADDRTLA
                     AMRAIEAQLGELPAGYTSELNLNFAPWFRALADGCSAAALLIVDYGYERASYYHPERR
                     DGTLSCHYRHRVHFDPLVYPGLQDITAFVDFDAAADAAEAAGFEVVGLVSQGHFLLAN
                     GLLDAAQQEFAGDDQWRRLQVAQQVKALTLPQEMGEKFKVLALAKNRALQMPAMMREA
                     ALG"
     gene            661..1458
                     /locus_tag="VIW27_13020"
     CDS             661..1458
                     /locus_tag="VIW27_13020"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY5740641.1"
                     /translation="MDNLQIFALAVLQGLTEFLPISSSAHLVLLPQLLHWPDQGLVFD
                     VAVHFGSLIAVVFYFRAEVLSMLTAWIGSISGGQSNRDSQLAWWIIIGTLPAVCAGFL
                     LQGDIESQLREPWIVAAASIGFGLLLWFADATSRRERDEFQLRLRDVLLIGFSQVLAL
                     IPGTSRSGITITAGLLLGLSRQAAARFSFLLAIPVIFASGVLESVRMFTEVSPISWGD
                     LLLGVVLSAISAGLCIHYFLRLVERLGMLPFVVYRVLMGVVILALLG"
     gene            complement(1664..2908)
                     /locus_tag="VIW27_13025"
     CDS             complement(1664..2908)
                     /locus_tag="VIW27_13025"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY5740642.1"
                     /translation="MKIYRVGGAVRDRLLGLPLQDIDWVVTGATPQQMLALGYKSIGK
                     DFPVFLHPQSKQEYALARSERKTGPGYRGFEIDADPETTIEQDLLRRDLTINAIAEDE
                     DGNLVDPCGGQRDIAERRLRHVSEAFTEDPVRVLRTARFAARYHHLGFTLAPETRELI
                     ESMGGSGELEALVAERVWSELSRALDEPSPQQFFLTLRDCHVLVHLFPEIDALFGVPQ
                     TAKYHPEIDTGVHLMLALQKSAELGQDNETRFAVLMHDLGKATTSPDILPGHYGHEQR
                     GVKLVKAFCERWRVPKSHTKLALITTEFHTLVHRAFDLKPSTLLDLLTRADSLRKPRR
                     FQRMLDACLADLRGRTHFENAPYPQADFVARLAESLRAMDTGDIRRQGLSGAALGDAI
                     RNARLELIKREKQRARTLDEHP"
     gene            complement(2905..>3312)
                     /locus_tag="VIW27_13030"
     CDS             complement(2905..>3312)
                     /locus_tag="VIW27_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740643.1"
                     /translation="LQPVYVGDLVKRIVTAIDDRDSYGKRYPVCGPEVFTLRQILELI
                     IAELHLPVRILPLGNGLSRLQAMIVQNLPGKLFTMDNYRSLQTPNVCENGNPGSTSLR
                     QYLKGLPAMFGNKRDYDRFRSDYPSVVPPEDRP"
BASE COUNT          531 a         1005 c         1122 g          654 t
ORIGIN      
        1 tcgccaacga ggtactcgac gcgatgccgg tgaagcgtgt cgtgcagcgt gagcgctggc
       61 gggaacttgg cgtcgggctt gacggcggcc gtttccagtg gcgcgagctc gatgccgatg
      121 accgcacgct cgcggcaatg cgcgcgatcg aggcgcaact cggcgaattg ccggcgggct
      181 acaccagcga attgaacctg aatttcgcgc cctggttccg cgcgctggcc gacggctgca
      241 gcgccgccgc gctactgatc gtcgattacg gctacgaacg ggccagctat taccatcccg
      301 agcgccgcga cggcaccctc agttgtcatt accgccaccg cgtgcatttc gacccgctgg
      361 tctatcccgg attgcaggat ataaccgcct tcgtcgattt cgatgccgcc gcggatgccg
      421 ccgaagccgc cggattcgag gtcgtcggcc tggttagcca gggtcatttc ctgctcgcca
      481 acggcttgct cgacgcagcg cagcaggaat ttgccggcga cgatcaatgg cggcggctgc
      541 aggttgcaca gcaggtcaag gcgctgacgc tgccgcagga gatgggcgag aaattcaagg
      601 tactggcgct ggcgaagaat cgggcgctgc agatgccggc gatgatgcgc gaggcggccc
      661 ttggataacc tgcagatctt tgcgcttgcg gtgctgcagg ggctcaccga attcctgccg
      721 atttcgagct cggcgcacct ggtgctgttg ccgcaattgc tgcactggcc cgaccagggg
      781 ctggtattcg atgtcgcggt ccatttcggc tcgctgattg cggtggtgtt ttattttcgc
      841 gccgaggtgc tgagcatgct gacggcctgg atcgggtcga tcagcggcgg ccaaagcaat
      901 cgcgacagcc agcttgcctg gtggatcatc atcggtacgc tgccggcggt gtgcgccgga
      961 ttcctgttac agggcgatat cgagagccag ttgcgcgaac cctggatcgt cgccgccgcg
     1021 tcgataggct tcggtctgct gctgtggttc gcggacgcca cctcgcgccg tgagcgcgac
     1081 gagttccagc tgcgcctgcg agacgtgctg ctgatcggct tcagccaggt gctggcgttg
     1141 atacccggaa cctcgcgctc gggaatcacg atcaccgccg ggctgctgct cggcctgtcg
     1201 cggcaggccg cggcacgctt ttcgttcctg ctggcgatac cggtgatttt cgccagcggc
     1261 gtgctggaga gcgtgcgcat gttcaccgag gtcagcccga tctcgtgggg cgacctgctg
     1321 ctcggcgtgg tgttgtcggc gatcagcgcc ggcctgtgca ttcactattt tttgcggctc
     1381 gtcgagcggc tgggcatgtt gccgttcgtc gtctaccgtg tgctgatggg cgtcgtgatc
     1441 ctggcactgc tcggctaggc cggcgcatgt cgtcgaccgc gacagagact acccggcgcg
     1501 ccgttccgca actggcccgc gtcctgcagc gttagcccgc cgcttgaacg agccggctaa
     1561 tggctgtcgg gagcggctgg attgaccgtg cggcttgtcg cgaatgatgg cgaagccgtg
     1621 ggtgggcagc gccatggtta tcgcttcatg cctgcggcgt cgttcaggga tgctcgtcca
     1681 gggtgcgcgc tcgctgcttt tcccgcttga tcaactctag cctggcattg cgaatcgcat
     1741 cgccgagcgc cgcgccgctg agcccctgtc ggcgaatgtc gccggtgtcc atggcgcgca
     1801 gggattcggc cagccgcgca acaaaatcgg cctgcgggta aggcgcgttc tcgaagtgcg
     1861 tacggccgcg cagatcggcg agacaggcgt cgagcatgcg ctgaaaacgc cgcggtttgc
     1921 gcaggctgtc ggcccgggtc agcaaatcga gcagcgtcga cggcttgagg tcgaaggcgc
     1981 gatgcaccag ggtatggaac tcggtggtga tcagtgccag cttggtatgt gatttcggta
     2041 cccgccagcg ctcgcagaag gccttcacca gcttgacgcc gcgttgttcg tggccgtagt
     2101 ggccgggcag aatatcgggc gaggtcgtgg ctttgccgag atcgtgcatc agcaccgcga
     2161 agcgggtttc gttgtcttgc ccgagttcgg cgcttttttg cagtgccagc atcagatgca
     2221 ccccggtatc gatctcggga tggtatttcg cggtttgcgg gaccccgaac agtgcgtcga
     2281 tctcggggaa caggtgcacc agcacgtggc agtcgcgcag ggtcaggaaa aactgctgcg
     2341 gtgacggctc gtcgagggcg cgggataatt ctgaccagac gcgttcggcg accagggcct
     2401 cgagttcgcc cgagcccccc atggattcga tcagttcgcg ggtttcgggc gccagcgtaa
     2461 agccgagatg atgataacgg gccgcgaaac gggcagtgcg cagcactcgc accgggtcct
     2521 cggtaaaggc ctcggacacg tgccgcaggc gccgctcggc aatgtcccgc tggccgccgc
     2581 agggatcgac cagattaccg tcctcgtcct cggcaatagc attgatcgtc aggtcacggc
     2641 gcagcaggtc ctgctcgatg gtggtctcgg gatcggcgtc gatctcgaat ccccggtaac
     2701 ccggcccggt cttgcgctcg ctgcgcgcca gcgcgtactc ctgcttgctt tgcgggtgca
     2761 ggaaaaccgg gaaatccttg ccgatcgact tgtagccgag ggccagcatc tgctgcgggg
     2821 tggcgccggt gaccacccag tcgatgtctt gcagcggtaa accgagcagg cggtcgcgaa
     2881 cggcgccgcc gacacggtat atcttcacgg gcggtcctct ggagggacga ccgaggggta
     2941 gtcgctgcga aagcggtcat aatcgcgctt gttgccgaac atcgccggca accccttcag
     3001 gtactggcgc aggctggtcg aaccaggatt gccgttctcg cagacgttgg gggtttgcag
     3061 cgagcggtaa ttatccatcg tgaacagctt gccgggcagg ttttgcacga tcatcgcctg
     3121 caggcgcgac aagccgttgc cgagcggcag aatacgcacc ggtaaatgca gctcggcgat
     3181 gatcagttcg agtatttgcc gcagcgtaaa cacttccgga ccgcagaccg ggtaacgctt
     3241 gccatagctg tctcggtcat cgatggcagt gacgatccgc ttgaccaggt cgccgacgta
     3301 caccggctgc ag
//
LOCUS       DATCZV010000576         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_152459, whole genome shotgun sequence.
ACCESSION   DATCZV010000576 DATCZV010000000
VERSION     DATCZV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152459"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VIW27_13035"
     CDS             <1..1018
                     /locus_tag="VIW27_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5740644.1"
                     /translation="HAGHSASRLSRFNEDEFAFLGATDGNAASDYLEALFEQFRRQVG
                     RVPELTGARLVAGVCNIQADDEVSRILARVDYSLSQASNRGPFSIERQIAANLDLPQG
                     KMQWRGWLDTVIDKQRLFLVGQLAISNDRIPIQRELFVRARGDGDQVIPAAVFIPMAA
                     SLGMSLDIDRAVFRLITSAAQLDRRIPLAINLSAAFFELAEAQEDFNHLLEHCRQNDM
                     RLCIEASHNALLRSPAMCSQVSERVRSYGHSFGIDNLDFGQSLQLLQSGEFDYVKVNA
                     RVLHDLVENEMTASYQALRTLGDTLDLRIIAVAVDSQQVHDNLRELGIENMQGNLLGE
                     HEVI"
     gene            complement(1049..1483)
                     /locus_tag="VIW27_13040"
     CDS             complement(1049..1483)
                     /locus_tag="VIW27_13040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5740645.1"
                     /translation="MSDRQQAETRDGYRHFLTITTRWMDNDLYGHINNVQYYSYFDTV
                     VNRFLIERGVLDIHGGEVVGLVVETHCNYFSEAAFPDDIEAGLRVAQLGRSSVRYEVG
                     LFVDGRAAAIAQGHFVHVYVDRHSRRPVELPSALRAALAAIE"
     gene            complement(1800..>2102)
                     /locus_tag="VIW27_13045"
     CDS             complement(1800..>2102)
                     /locus_tag="VIW27_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEY5740646.1"
                     /translation="HVDLVGSFTPRMREADDAVLRRSRLFVDHRQSAGRSGEFIGPFE
                     RGVIGADDIRGDLFELCQGRVAGRTSAEEITTMKNGGGSHLDYFVSRYLLECLGRG"
BASE COUNT          417 a          680 c          618 g          387 t
ORIGIN      
        1 gcacgccggg cacagcgcca gccgcctgtc gcgcttcaac gaggatgaat tcgcgttcct
       61 cggcgccacc gacggcaacg cggcgagcga ctacctcgaa gcgctgttcg agcagttccg
      121 gcggcaggtc ggcagggttc cggaattgac cggcgccagg ctggtagccg gtgtttgcaa
      181 tattcaagcc gacgacgaag tctcgcgaat actggcccgg gtcgactact cgctgtcgca
      241 ggccagcaac cgggggccat tttccatcga gcgacagatt gccgccaacc tcgatctgcc
      301 acagggcaaa atgcagtggc gcggctggct ggatacggtg atcgacaagc agcggctgtt
      361 cctcgtcggc cagctcgcga tcagcaacga tcgcattcca atacagcgtg aactcttcgt
      421 ccgcgcccgc ggcgacggcg accaggtgat accggcagcg gtattcattc cgatggccgc
      481 cagcctcggc atgtcgctgg atatcgaccg tgcggtattt cgcctgatca cctcggccgc
      541 acagctggac cgccgcattc cgctggcgat caatctcagt gccgcgtttt tcgagctggc
      601 cgaggcccag gaagatttca atcacctgct cgaacactgc cggcaaaacg acatgcggct
      661 atgcatcgag gccagtcaca atgcgctgct gcgctccccg gccatgtgca gccaggtgtc
      721 cgaacgggtg cgcagctatg gccacagttt cggcatcgac aatctcgatt tcggccagtc
      781 cctgcaactg ctgcaatccg gcgaattcga ctatgtgaaa gtcaacgccc gggtattgca
      841 cgacctggtg gagaacgaga tgaccgccag ctaccaggca ttgcgcaccc tcggcgatac
      901 gctcgatctt cgcatcatcg cggtcgcggt cgattcgcag caagtccatg acaatctgcg
      961 cgaactcggc atcgagaaca tgcagggcaa tctgctcggc gaacacgaag tcatctaggc
     1021 cgccgaagcc ggcacccggc aatactcgtc actcgatcgc ggccagcgcc gcccgcagtg
     1081 ccgacggcag ttccaccggc cgccggctgt gccggtcgac gtagacgtgc acgaaatggc
     1141 cctgcgcaat tgccgcggcg cggccgtcga cgaacaaccc cacctcatag cgcaccgagc
     1201 tgcgccccag ctgcgcaacc ctgagccccg cctcgatatc gtcgggaaag gcggcctcgc
     1261 tgaagtaatt acagtgggtt tcgaccacca ggccgacgac ttcaccgcca tggatatcga
     1321 gcaccccgcg ctcgatcagg aagcggttga ccacggtatc gaaataactg tagtactgca
     1381 cgttgttgat atgcccgtag aggtcgttat ccatccagcg cgtggtgatg gtcagaaaat
     1441 gccgatagcc gtcgcgtgtt tcggcttgct gcctgtcgga catgcgcgta ccccgtggtc
     1501 tcagatcaat agatttaata gctcgatagc ggcgataaaa actgtcatta accgctcgac
     1561 ccgatttccg gtagagtgta atcggttatg cggcccaaca cattcctgat cctgttcgtg
     1621 atcatgtgga tcgttttcag cctgtcagtc tgaatccgca cgacacgcca gcattaaagc
     1681 ggcctattcg tcgggcgcag gccgcacccg ctatgccgaa aaccggcgca aaatcaacga
     1741 acccgctgct tgaataccgg caaccccgcg gcgggcttcg gcctgacggc cgcggcgcgc
     1801 tagccgcggc cgaggcactc gagcagatag cggcttacga agtagtcgag atggctgcca
     1861 ccgccgtttt tcatcgtcgt gatttcctcg gccgaggttc tgccggccac cctgccctgg
     1921 caaagctcga acaggtcgcc gcgaatgtcg tccgcgccga tgacgccgcg ttcgaacggt
     1981 ccgatgaatt cacccgagcg gccagcggac tggcggtgat cgacgaacag tcggctgcgt
     2041 cgcagtaccg cgtcgtcggc ttcacgcatc ctcggggtga aggatccgac caggtcgaca
     2101 tg
//
LOCUS       DATCZV010000577         8363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_577996, whole genome shotgun sequence.
ACCESSION   DATCZV010000577 DATCZV010000000
VERSION     DATCZV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577996"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VIW27_13050"
     CDS             complement(<1..199)
                     /locus_tag="VIW27_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740647.1"
                     /translation="MSRYRDIALLAAILMLSACGGQPGPTPLDDAQTPRQLAESQSAK
                     ERSQAPQEPAPPDDTRTAAALD"
     gene            complement(183..3011)
                     /locus_tag="VIW27_13055"
     CDS             complement(183..3011)
                     /locus_tag="VIW27_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5740648.1"
                     /translation="MNRRLRQGLLVACGAALLASCAAERDDAIGPTLADLENLPPIID
                     SVELEPRASFEIDRQQVIDSYRELVAITAGGGGTGDEMRRLAELELESSLDKRDSEDP
                     EVRQQGLESAQRAISIYEDYLEKYPARADNDTVLYQLSRAYAIDGENAQSAALLERIV
                     RDYTDSRYMAEVQFRRGEYLFVEREYEAAENAYGSVVNDYPESLFFDKSLYKYGWAQF
                     KRGNHLDALASYLRLLDLNFEQGRIEQIEFSPELSRADRELLDDVVRVVALNFAYQND
                     TNFIAGYFADNGKRDYEPLLYRRLGELFLDQERILDSASLFLTYTAEYPYSQFTPYFH
                     QRAIETYEQAGYTDLVLREKIAFVDRYEVLGEYWMKQNPVTQERLAATLAVHLTDLAT
                     HFHALARSSGDRGDYRVAADWYRRFLASFAEHPRAPRMNFLLAESLFDARRYPQAIDE
                     YHKTAYDFPPHADSAEAGYAALLAFDKLFETTNASEFEALRRKRIQSAVRFTTGFPAD
                     PRLAAIELQTAQQFFDWQQYGEAITSAERLIGSDGIDQPTRKSAWAIVADAQFLLADY
                     AAAETSYHQLLELTPRQGSEPGEVREQIAASIYKQGEAARAAGELLLAAGHFSRLGES
                     VPASPTRIVADYDAASVYVELQDWPRAISQLEDFRRRYPRDRRFAEGVTESLALAYSS
                     SGEQLLAAREMLVLSKYPGSADRRQDLMWRAAEIYQQSGQSARAIEIYKDYVAAYPYP
                     LERSMELRYQIAESYRAAGDNANLRRWLEAIVRADAAAGTRSNARSKYLAATASLELI
                     EPLQRSYRQVELTVPLKTSLQKKKQLMQKSIDAYSRAIKYQVEEVTTEATFQIAEIYR
                     HFARSILDSQRPRGLDEEQLEEYELLLEEQAYPFEEKAIDIHLANFKRIPAGTYDEPT
                     RNSLRALGEMMPYRFAKVEGTENYVEIP"
     gene            complement(3008..4828)
                     /locus_tag="VIW27_13060"
     CDS             complement(3008..4828)
                     /locus_tag="VIW27_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740649.1"
                     /translation="MLAAVLGGAAAAQELDRHRVDDLDYGEALYHYFQQQELAAITRL
                     MIAEQSLRPRRQQDESRLLLADLYYGYGLYAESREMFARLLTSKVSDPVQNRIWFSLA
                     RLRYEQGYTEQASDLLARITDSLPERIEAERKYLMTNLHLANRRYQQAADISNRIDSK
                     SIWKTYARYNLAVSVIEDAGYEQGRQLLEKIGTMPADASISSAELLALRDQANLSLGL
                     KQLRMGKNRQALDSLSRIRLQGPLSHEALLASGWAWQRLRQPQKALLPWRLLLRRNAV
                     DAATQEAILAIPSSYAALGEDRLALQHFEIAARQFDAQLRLLDDAVASIENDELIASL
                     REHALLFDRSSLQRLPPSSEVTAQLHLLLASSGFQQEMRRYQQLLDIEQSLRYWDNSF
                     PALGLMLDERRQAFRQRLPKLGESTGFARLEELKQQRAEYARRIREIEVREDHRALAT
                     DEEREHLERLQRAAESIDRIGGARDTVDQQDKLRLISGLLDYRLASEFPARFWKAKKQ
                     LIELDRALADGERSAQSLRRIVDSNEQEMDGFERRIDGQLERIGRLRGAVSELLRRQE
                     QRINQLAIDALRRQQEHVTQLRLNARFELAKLYDKLATEQ"
     gene            complement(4887..5432)
                     /locus_tag="VIW27_13065"
     CDS             complement(4887..5432)
                     /locus_tag="VIW27_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5740650.1"
                     /translation="MKTNSLIALLLLALALPLAAQEPADGVAAEAPPGGSLDADIEAL
                     KKEVLSLNRDLFILEEDLLFPANTQFSVFLSMDAGQLFTLDSVQLKIDDKNIANHLYT
                     ERELAALQRGGVQRLYIGNLSSGEHEIIAIFTGIGPQGRDYRRGESIVVEKTSEPQFV
                     EFMIIDDITKEQPRFDVRVWE"
     gene            complement(5419..6474)
                     /locus_tag="VIW27_13070"
     CDS             complement(5419..6474)
                     /locus_tag="VIW27_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5740651.1"
                     /translation="MPISRCDRRYRPPRYRCRLCGLLLAAAAIAPAPAAQWHHQQREM
                     MGTRISIDVEHADAALAADCSERVFAEMRRVEALMSTYREDSEISRVNDQAAAGDVEI
                     STELASLIASSIRFSRLSAGVFDITYASIGYAYDFRQQRQPDAATLAARLPAVDYRHI
                     ELSDNRIHFSRAGVRIDLGGIAKGYAVDRAAEITRRCGITRAMISAGGDSRIIGDRDG
                     RPWMIGIRHPREAGGIALRLPLSDTAISTSGDYERYFMADGERIHHILNPATGRPSQS
                     SWSATVIGPDAMTTDALSTTIFILGADDGIALIEKLEGFDAIVIDSSGKVHYSSGFEE
                     PDAVPTQTPEPLADENE"
     gene            complement(6490..8145)
                     /locus_tag="VIW27_13075"
     CDS             complement(6490..8145)
                     /locus_tag="VIW27_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740652.1"
                     /translation="MCRPSKNTYLPPDAALKFEDHGRPLSRRQFIRQGFLTGSATLLS
                     GGLFGLFANPNAAYAEVADDLQSLVDDINLRGNINCSVGGLAAGQKVPFICFDLAGGA
                     NLAGSNVLVGEGGGQFDLLSTAGYRKLGLPGDMLPGQVDTGITLLNPSNGDFTNTDLG
                     LAFHADSAMLRGILDKALTAVGNIDGCVIPARSDNDTGNNPHNPLYALAMSGARGEVV
                     DLVGSRSSISGGNSLSPAMYVNPEFRPTKVDRPSDVTGLVDVGNLTAILGADDVTAVM
                     ESIARVAHKKLGRVDTGLGVSGDEVVKDLVRCGYIKSADLAQSFAGRVIDPTQDLDIL
                     SIFSSTELNNDSEFRKTASVMKMVLNGFAAGGCITMGGYDYHTGDRITGENRDLRAGR
                     CIGACLEYARLLGKPLMVYVFSDGSVFSNGNTDDSLAPAPNATGLPTVGGGGKGEWTG
                     DNSSTACSFFLVFDPAGPPSLIGSRQIGRMSADASVVTAATPAANNVNLLVNLVMLNF
                     MALNDPLAAGFGDVISWYKAAFPNHGLGNDAALASFITFDRIA"
     gene            complement(8156..>8363)
                     /locus_tag="VIW27_13080"
     CDS             complement(8156..>8363)
                     /locus_tag="VIW27_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740653.1"
                     /translation="TQPDASEIGGLLGSDSPQDLDPAIAGITTYNSLIEQMLVGSANT
                     SQRTGQIVKAVCAAATGAAVMLVQ"
BASE COUNT         1375 a         2554 c         2811 g         1623 t
ORIGIN      
        1 catcgagcgc cgctgcagtt cgcgtgtcgt ctggtggcgc cggttcctgc ggcgcctgtg
       61 atcgctcctt cgcggactgc gattccgcga gttgccgcgg ggtttgcgcg tcgtcgagcg
      121 gcgtggggcc cggctggccg ccgcaggccg acagcatcag gatggctgcc agcagcgcga
      181 tgtcacggta tctcgacata attctccgta ccttctacct tggcaaagcg gtaaggcatc
      241 atttctccga gcgctcgcag gctgttgcgc gtcggttcgt cgtaggtgcc ggcggggatg
      301 cgcttgaaat tggcgaggtg gatatcgatc gccttttcct cgaacgggta agcctgttcc
      361 tcgagcagca gttcgtactc ctcgagctgt tcttcgtcga gaccgcgcgg ccgctgcgag
      421 tcgagaatcg agcgcgcgaa atgacgatag atctcggcga tctggaaggt cgcctcggtg
      481 gtcacctcct cgacctggta cttgatcgcg cggctgtagg catcgatcga tttttgcatc
      541 agctgcttct ttttttgcag actggtcttc agcggcacgg tcagctcgac ctgccggtaa
      601 gagcgctgca gcggctcgat cagctccagg ctggcagtag ccgcgaggta cttgctgcgc
      661 gcattgctgc gcgtgccggc agcggcgtca gcgcgtacga ttgcctcgag ccagcggcgc
      721 aggttggcgt tgtcaccggc ggcacggtat gactcggcga tctggtagcg cagctccatc
      781 gagcgttcca gcgggtaggg ataggccgct acgtaatcct tgtagatttc gatcgcgcgc
      841 gcggattgtc cactctgctg gtaaatttcc gcggcgcgcc acatcagatc ctggcggcga
      901 tcggcgctgc cgggatactt cgacaggacc agcatttcac gcgccgcgag cagctgctct
      961 ccggaactgc tgtaagcgag cgcgaggctt tcggtcaccc cctcggcgaa acgccggtcc
     1021 cggggatagc gccgtcgaaa atcctcgagt tggctgatag cccgtggcca gtcctgcagt
     1081 tcgacgtaga cgctggcggc gtcgtagtcg gcgacgatgc gcgtcggcga tgcgggcacg
     1141 gattcaccga gacgcgaaaa atgcccggcc gccagcagca gttcgccggc ggcgcgcgcg
     1201 gcctcgccct gcttgtagat gctggcagcg atctgctcgc gtacctcgcc aggttcgctg
     1261 ccctgccgcg gcgtcagctc gagcagctgg tggtaggagg tctcggcggc ggcatagtcg
     1321 gcaagcagga actgcgcgtc ggcgacgatc gcccaggccg actttcgggt cggctggtcg
     1381 atgccgtcgc tgccgatcag gcgttcagcc gaggtgatgg cttcgccgta ttgctgccag
     1441 tcgaaaaact gctgcgcggt ctgcagctcg atagccgcca gtcgcgggtc ggccggaaag
     1501 ccggtggtga agcgcaccgc cgactgaatg cgcttgcgcc gcaatgcctc gaactcgctc
     1561 gcattggtgg tctcgaacag cttgtcgaag gcgagcagcg cggcgtaacc ggcttcggcg
     1621 ctgtcggcgt gcggcgggaa gtcgtaagcg gtcttgtggt attcgtcgat ggcctgcggg
     1681 tagcggcgcg catcgaacag gctttcggcg agcagaaagt tcattcgcgg cgcgcgcggg
     1741 tgttcggcaa acgaggccag gaagcgacgg taccagtcgg ccgcgactcg gtaatcgccg
     1801 cggtcgccgc tgctgcgcgc gagcgcgtga aagtgcgtcg ccaggtcggt caggtgcacc
     1861 gccagcgtgg ctgccagtcg ctcctgggtt accggattct gcttcatcca gtattcgcca
     1921 agcacttcgt aacggtcgac gaaggcgatt ttttctcgca acaccaggtc ggtatagccg
     1981 gcctgctcgt aagtctcgat cgctcgctgg tggaagtagg gcgtgaactg agaatagggg
     2041 tactcggcgg tgtaggtcag gaacaggctg gcgctgtcga gaatccgctc ctggtcgagg
     2101 aagagttcgc cgaggcggcg atagagcagg ggttcgtaat cgcgcttgcc attgtcggcg
     2161 aaatacccgg caatgaaatt tgtgtcgttc tggtaggcga aattgagcgc caccacgcgc
     2221 accacgtcgt cgagcaattc acgatcggcg cgcgacagct cggggctgaa ctcgatctgc
     2281 tcgatccgcc cctgctcgaa gttgaggtcg agcaggcgaa ggtagctcgc cagagcgtcg
     2341 aggtgattgc cccgcttgaa ctgggcccag ccatacttgt acagggactt gtcgaaaaac
     2401 agcgattcgg ggtagtcgtt gacgacgctg ccgtaggcgt tttccgcggc ctcgtactcg
     2461 cgctcgacga acaggtactc gccgcgccgg aactggacct cggccatgta cctggaatcc
     2521 gtgtagtcgc ggacgatgcg ctccagcaag gccgccgatt gcgcgttttc gccgtcgatc
     2581 gcatatgcgc gcgataactg gtatagaacc gtatcgttgt cggcgcgtgc cgggtacttt
     2641 tccaggtagt cttcgtatat ggagatcgct cgctgggcgc tctccaggcc ctgctggcga
     2701 acttccggat cctcggaatc gcgcttgtcg agactcgatt ccagttcgag ttcggccagg
     2761 cgccgcattt cgtcgccggt accgccgccg ccggcggtga tcgccaccag ctcgcgatag
     2821 ctgtcgatga cctgctggcg gtcgatctcg aagctggcgc gcggttccag ttcgacgctg
     2881 tcgataatcg gcggcaggtt ttcgagatcg gcgagggtcg gcccgatagc gtcgtcgcgt
     2941 tcggcggcgc acgaagccag cagcgccgcg ccgcaggcca caagcaggcc ctgccgtagc
     3001 cgccggttca ttgttccgtc gccagtttgt cgtacagctt ggccaactcg aaccgagcgt
     3061 tgagacgcag ctgcgtgacg tgttcctgct ggcggcgcaa ggcgtcgatc gccagttgat
     3121 taatgcgctg ttcctggcgt cgcagcagct cgctcaccgc gccgcgcaac cggccgatgc
     3181 gctccagctg tccgtcgatc cgccgctcga agccgtccat ttcctgttcg ttgctgtcga
     3241 cgatgcggcg cagcgattgc gcgctgcgct caccgtcggc gagcgcgcga tccagctcga
     3301 tcaattgctt tttggctttc cagaagcgtg ccgggaactc gctcgccagc cggtaatcaa
     3361 gcagtcccga gatcaggcgc agcttgtcct gctggtcgac ggtgtcacgc gccccgccga
     3421 tcctgtcgat cgactccgcg gcgcgctgca ggcgctcgag atgctcccgc tcttcgtcgg
     3481 tggcgagtgc gcgatgatcc tcgcggacct cgatttcgcg aatccggcgg gcgtattcgg
     3541 cgcgttgctg cttgagttcc tcgagccgcg cgaagccagt cgattcgccg agcttcggca
     3601 gccgttgcct gaaggcttgc cggcgctcgt cgagcatcag gccgagtgcc gggaagctat
     3661 tgtcccagta gcgcagcgat tgctcgatat cgagcagctg ctggtagcgg cgcatttcct
     3721 gctgaaatcc ggaagacgcc agcagcaggt gcagctgtgc ggtcacttcc gagcttggcg
     3781 gcaggcgctg caggctggag cgatcgaaca acagcgcgtg ttcgcgcagc gaggcgatca
     3841 attcgtcgtt ttcgatcgac gcaacggcgt cgtcgagcaa ccgcagctgg gcatcgaact
     3901 ggcgcgcggc gatctcgaaa tgctgcagcg cgagccggtc ctcgccgagc gccgcatagc
     3961 tcgacggaat cgccaggatc gcttcctggg tagccgcgtc gacggcatta cgcctcagca
     4021 gcaggcgcca cggcagcagc gctttttgcg gctggcgcaa gcgctgccag gcccatcccg
     4081 aggccagcag ggcctcgtgg ctgagcggcc cctggaggcg aatccgcgac aggctgtcga
     4141 gagcctgacg gttcttgccc atgcgcaact gcttcaatcc cagcgacagg ttggcctgat
     4201 cgcgcagcgc cagcagctcg gccgagctga tcgatgcatc cgccggcatg gtgccgattt
     4261 tttccagcag ttgccggccc tgctcgtaac cggcgtcctc gatcaccgat actgccaggt
     4321 tgtagcgcgc gtaggtcttc cagatcgact tcgaatcgat gcgattgctg atgtccgcgg
     4381 cttgttggta gcggcgattc gccaggtgca ggttggtcat caggtacttg cgctcggcct
     4441 cgatacgctc cggcagggag tcggtgatcc gcgccagcag gtcgctggcc tgctcggtat
     4501 agccctgctc gtagcgcagc cttgcgaggc tgaaccagat gcggttctgc accgggtccg
     4561 ataccttcga ggtcagcagg cgggcgaaca tttcgcgcga ttcagcgtac agtccgtagc
     4621 cgtagtagag gtcggcgagc aacagccgag attcgtcctg ctgccggcgc gggcgcaggc
     4681 tttgctcggc gatcatcaag cgggtgatcg ccgccagttc ctgttgctga aaatagtgat
     4741 agagcgcctc gccatagtcg agatcgtcga cgcggtggcg gtcaagctcc tgcgctgcgg
     4801 cggcgccgcc aagcacagcg gccagcacgg ccggcagcca gacggccagc cgcgcgcaaa
     4861 gcctgcggtg gcgggtcggt cgcgggttat tcccagacgc gcacatcgaa gcggggttgt
     4921 tccttggtga tatcatcgat gatcatgaat tcgacgaatt gcggctcgct ggtcttttcg
     4981 acgacgatcg attcgccacg ccggtaatcc cgcccctggg gtccgatgcc ggtgaaaatg
     5041 gcgatgattt cgtgttcccc cgaagacagg ttgccgatat acaggcgttg tacgccgccg
     5101 cgctgcaggg ccgccagctc gcgctcggtg tagaggtggt tggcgatgtt cttgtcgtcg
     5161 atcttgagct gcaccgaatc gagcgtgaat agttgccctg cgtccatcga caggaaaacg
     5221 ctgaactgcg tgttcgccgg aaacagcagg tcttcctcga gaatgaagag gtcgcgattg
     5281 agcgacagaa cttccttttt gagcgcctcg atgtcggcgt cgagcgaacc gccggggggg
     5341 gcttcggcgg caacgccgtc ggcgggctcc tgtgctgcca gcggcaatgc cagggcaagc
     5401 agcagcaggg ctatcagact attcgttttc atcggctaac ggctcgggcg tttgcgttgg
     5461 tacggcgtcg ggttcctcga agccggacga atagtgtacc ttgccgctgc tgtcgatgac
     5521 gatggcgtcg aagccttcca gcttttcgat cagcgcgata ccgtcgtccg ctccgaggat
     5581 gaaaatggta gtggataacg cgtcggttgt catcgcgtcg gggccgatca cggtcgcgct
     5641 ccagctactt tgcgacggcc ggccggtggc cgggttgagt atatggtgta tgcgctcgcc
     5701 gtcggccatg aaataacgct cgtagtcgcc cgaggtcgag attgcggtgt cgctgagcgg
     5761 caggcgtagc gcgatgccgc cggcctcgcg tggatggcga atgccgatca tccacggccg
     5821 gccgtcgcgg tcgccgatga tgcggctgtc gccgccggca ctgatcatcg cgcgcgtaat
     5881 gccgcagcgc cgggtgatct cggccgcgcg gtcgaccgca taacccttgg cgataccgcc
     5941 gagatcgatg cgcaccccgg cgcgtgagaa gtgtatgcgg ttgtcgctta actcgatatg
     6001 ccggtagtcg accgccggca gccgtgccgc cagggtcgcg gcgtcgggct ggcgctgctg
     6061 gcgaaaatcg taggcatagc cgatgctcgc gtaagtgata tcgaagaccc ccgccgagag
     6121 ccgcgaaaat cggatcgagc tggcgatcag cgacgccagc tcggtgctga tctcgacatc
     6181 gccggccgcc gcctggtcgt tgacgcggct gatctcgctg tcttcgcggt aggtgctcat
     6241 cagcgcctcg acgcggcgca tttcggcaaa tacgcgttca ctgcaatccg ccgccagcgc
     6301 ggcatcggca tgttcgacgt cgatcgagat gcgcgtgccc atcatttcgc gctgctggtg
     6361 gtgccattgc gctgccggcg ccggagcgat cgccgccgcg gccagcagca ggccgcaaag
     6421 ccggcatctg taacggggag ggcggtaacg acgatcgcac cgggaaattg gcatggacga
     6481 ggtcagtagt caggcgatac ggtcgaaagt gataaagctg gccagcgcgg catcgttgcc
     6541 gaggccatgg ttcgggaaag cggccttgta ccaggaaatg acgtcgccga aaccggcggc
     6601 gagcgggtcg ttcaaggcca tgaaattcag catcaccagg ttgaccagca ggttgacgtt
     6661 gttggcggcc ggcgtcgccg cggttaccac cgaggcgtcg gcgctcatgc ggcctatctg
     6721 ccgactgccg atcaggctgg gcgggccggc gggatcgaag accaggaaga acgagcaggc
     6781 ggtcgaggaa ttgtcaccgg tccactcgcc cttgccgccg ccgccgacgg tcggcaggcc
     6841 ggtggcgttg ggtgccggcg ccaggctgtc gtcggtattg ccgttgctga ataccgagcc
     6901 gtcgctgaat acgtagacca tcagcggttt gccgagcagc cttgcatact ccaggcaggc
     6961 gccgatgcag cggccggcgc gcaggtcgcg gttttcgccg gtgatgcggt cgccggtgtg
     7021 gtaatcgtag ccacccatgg tgatacaacc gccggcggcg aagccgttga gcaccatttt
     7081 cattaccgac gcggtcttgc ggaactcgct gtcgttgttc aattcggtac tcgaaaagat
     7141 cgacaggatg tcgagatcct gggtcggatc gatgacccgc ccggcgaaac tttgcgccag
     7201 gtcggcggac ttgatgtagc cgcagcgcac caggtccttg acgacttcgt cgccggaaac
     7261 accaagcccg gtatcgaccc ggcccagttt cttgtgcgcc acgcgggcga tcgattccat
     7321 caccgcggtg acgtcgtcgg cgccgagtat cgcggtcagg ttgccgacat cgaccaggcc
     7381 ggtgacgtcg ctcggccggt cgaccttggt gggacggaac tcgggattga cgtacatcgc
     7441 cggggacagc gagttgccgc ccgagatact gctgcgcgag ccgaccaggt cgaccacttc
     7501 gccgcgcgcg ccgctcatcg ccagcgcata gagcgggttg tgcggattgt tgccggtatc
     7561 gttgtcggag cgcgccggaa tgacacagcc gtcgatattg ccgaccgcgg tgagcgcctt
     7621 gtcgagaatg cccctgagca tcgcgctgtc ggcatgaaat gcgagcccga gatcggtatt
     7681 ggtgaagtcg ccgttgctcg ggttgagcaa ggtgatgccg gtgtcgacct gtccgggcag
     7741 catgtcgccg ggcagaccga gcttgcgata accggctgtc gatagcaggt cgaactggcc
     7801 gccgccttcg ccgaccagca cgttcgagcc cgccaggttg gcgccgccgg cgaggtcgaa
     7861 gcagatgaac ggcaccttct ggccggccgc gagaccgccg accgaacaat tgatgttgcc
     7921 gcgcaggttg atgtcgtcga ccagcgactg caggtcgtcg gcgacctcgg cataggcggc
     7981 attggggtta gcgaacaggc cgaataagcc gcccgacaac agcgtcgccg agccggtcag
     8041 aaaaccctgg cggatgaact gtcggcggct cagcgggcgg ccatgatcct cgaacttgag
     8101 cgccgcatcg gggggcaggt aggtattttt actcggtctg cacatcgtcg gttccttatt
     8161 gtaccagcat caccgcggca ccggtggcgg ccgcgcagac cgccttgacg atttgcccgg
     8221 tgcgctgcga agtattcgcg ctgccgacca gcatttgctc gatcaggcta ttgtaggtgg
     8281 tgatgcccgc gattgccgga tccagatcct gcggcgagtc cgaacccagc aggccgccga
     8341 tttcgcttgc atcgggctgc gtg
//
LOCUS       DATCZV010000578         2874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_206057, whole genome shotgun sequence.
ACCESSION   DATCZV010000578 DATCZV010000000
VERSION     DATCZV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206057"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..858
                     /locus_tag="VIW27_13085"
     CDS             70..858
                     /locus_tag="VIW27_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5740654.1"
                     /translation="MSDFKYLQFDRQGAIATITLNRPAAANGLDTPLVAEFRRAAQLC
                     DADPQLKVVVLRAAGERFFSVGGDIKEMLAHGDGVGEALHALADDLHAAIAALLRMNA
                     VLIVAVNGIAGGAGFSLALIGDLVLAAESASFTMAYTRAGLSPDGSSTWLLPRLVGLR
                     RAQELMLTNRTLDAAEAFEFGIVTRVVADAELQAECERLAAEVAAGAGLANAYVKKLL
                     LAGAGNDLQTQLDLEAQLLSQCAASPDGREGIQAFVDKRKPRFR"
     gene            862..1776
                     /locus_tag="VIW27_13090"
     CDS             862..1776
                     /locus_tag="VIW27_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5740655.1"
                     /translation="MGDFDQGAAYVDGQYLPVGEARIPLLDWGFLHSDATYDVAHVWR
                     GRFFRIDDYIHRFQASMQKLRMAIDYDAAQIRDIMFELVRLSGLRDAYVEVVCTRGIP
                     APGSRDPRTCENRFLAFAIPFVWIADDAHRARGLDVLISRQQRIPPQAVDPRIKNYHW
                     LDMTMALFEAYDSGEDTAVLVDADGNLVEGPGFNVFVRRGNTVITPERGVLQGVTRTT
                     VLELLAKETELEVQPGLLPASLAMSSDEAFITSTAGGVMPVTRISGKAVGDGKPGELT
                     ERLNRAYWALHDDPQFNTEVDYRGAEDE"
     gene            1769..2527
                     /locus_tag="VIW27_13095"
     CDS             1769..2527
                     /locus_tag="VIW27_13095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5740656.1"
                     /translation="MNDASKVALVTGAGSGIGRAVSCALLRAGYSTVLAGRRREPLDE
                     TVAQSGVDAASALCVPTDVADPGAVRALFDAAVKKFGRLDLLFNNAGAYTPGVPIEEL
                     AYEDWRGAVDVNLTGAFLCAQQAIRIMKAQQPRGGRIINNGSISAHAPRPLSVAYTAT
                     KHAITGLTKSISLDCRNDDIVCGQIDIGNAATPMTAAMPKGVLQANGERAVEPVMDVE
                     HVADAVLQMASLPLDANVLFMTIMANRMPFVGRG"
     gene            complement(2528..>2874)
                     /locus_tag="VIW27_13100"
     CDS             complement(2528..>2874)
                     /locus_tag="VIW27_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5740657.1"
                     /translation="QFWENFCIAVTKPEWTARQFESMPQQALIGELAALFATQPRDHW
                     QARLDGIDCCFEVLLADGDLAAQPQFAFRAAIDADGPRYPAWIDGRAIETGTEPRQIS
                     SPNDLDWHTPLK"
BASE COUNT          490 a          913 c          977 g          494 t
ORIGIN      
        1 ataccgaatt tcgcggccgt tattcggtat actgggcacc gggttttaca gacggggcgc
       61 cgcttcgaaa tgagcgattt taaatatctg cagttcgaca ggcagggcgc gatcgcgacg
      121 atcacgctga atcggcccgc agccgccaac gggctcgata cgccgctggt tgccgaattc
      181 cgccgggcgg cgcagctatg tgacgcggat ccacagctca aggtcgtcgt cctgcgcgcc
      241 gccggtgagc ggtttttcag cgtcggcggc gacatcaagg aaatgctggc gcacggcgac
      301 ggggtcggcg aagccctgca tgcgctcgcc gacgacctgc atgcggcgat cgcggcactg
      361 ctgcgcatga atgcggtgct catcgtcgcc gtcaacggca ttgccggcgg tgccgggttc
      421 agcctggcgc tgatcggcga cctggtgctg gccgccgaat cggcgagctt cacgatggcc
      481 tatacacgcg ccggactaag ccccgacggc agttcgacct ggctgctgcc gcggctggtc
      541 ggcctgcgcc gcgcgcagga actgatgctc accaatcgca cgctcgacgc cgccgaggcg
      601 ttcgagttcg gcatcgtcac gcgggtcgtc gccgacgccg aattacaggc ggaatgcgag
      661 cgcctggctg ccgaagtggc tgccggtgcg gggctggcga acgcctatgt gaagaagctg
      721 ctgctggccg gcgccggcaa cgatctgcaa acccaactgg atctcgaggc acagctgcta
      781 tcgcagtgcg cggcatcgcc ggacgggcgc gagggtatcc aggccttcgt cgacaagcgc
      841 aagccgcggt ttcgttgatc gatgggcgat ttcgaccagg gcgcggccta tgtcgacggg
      901 caatacttgc cggtcggcga ggcgcgcatt ccgctgctcg actggggctt cctgcactcg
      961 gatgcgacct acgatgtcgc ccacgtctgg cgcggcaggt tttttcgcat cgacgactat
     1021 atccaccgct tccaggcgag catgcagaaa ctgcgcatgg cgatcgacta cgacgccgcg
     1081 cagattcgcg acatcatgtt cgaactggta cggctatccg gcctgcgcga cgcctacgtc
     1141 gaggtcgtct gcacgcgtgg cattccggcg ccgggttcgc gtgatccgcg aacctgcgag
     1201 aaccgcttcc tggcgttcgc gattccgttc gtatggatcg ccgacgatgc gcatcgcgcc
     1261 cgcgggctgg acgtgctgat cagccgccag cagcgaattc cgccgcaagc cgtcgatccg
     1321 cggatcaaga attaccactg gctggacatg accatggcgc tgttcgaagc ctacgacagc
     1381 ggagaggaca ccgcggtgct ggtcgatgcc gacggcaatc tcgtcgaggg gccgggattc
     1441 aatgtcttcg tgcgccgcgg caacaccgtg atcacgccgg agcgcggcgt gctgcagggc
     1501 gtgactcgta ccacggtgct cgaactgctg gcgaaagaaa ccgaactcga agtgcagccg
     1561 ggcctgttgc cggcgtcgct ggcgatgtcg tcggacgagg cgttcatcac cagcaccgcc
     1621 ggtggcgtga tgccggtgac gcgtatttcc ggcaaagcgg tcggcgacgg caagcccggc
     1681 gagttgaccg agcgactgaa ccgggcctac tgggcgctgc acgacgaccc gcaattcaac
     1741 accgaggtcg attatcgagg agccgaagat gaatgacgca agcaaggtag cgctggttac
     1801 cggtgccggc agcggtatcg gccgcgccgt cagctgcgcg ctgctgcgcg ccggctatag
     1861 tacggtgctc gccggccggc ggcgcgagcc gctcgacgaa accgtcgcgc agtccggcgt
     1921 ggatgccgcg tcagcgctat gcgtgccgac cgacgtcgcc gaccccggtg cggtgcgtgc
     1981 gctgttcgac gcggcggtga agaaattcgg ccgcctcgac ctgctgttca acaatgccgg
     2041 cgcctacacg cccggggtgc cgatcgagga actggcttat gaagactggc ggggcgccgt
     2101 cgacgtcaac ctgaccggcg ccttcctgtg cgcgcagcag gcgatccgca tcatgaaggc
     2161 gcagcagcca aggggagggc gcatcatcaa caacggctcg atctcggcgc atgcgccgcg
     2221 cccgctgtcg gtggcctaca cggcgaccaa gcacgcgatt accgggttga ccaaatcgat
     2281 ttcgctggat tgccgcaacg acgatatcgt ctgcggccag atcgacatcg gcaacgcggc
     2341 gacgccgatg accgcggcga tgccgaaggg cgtattgcag gccaacggcg agcgcgcggt
     2401 agagccggtg atggacgtcg agcacgtcgc cgacgcggtg ctacagatgg cgagcctgcc
     2461 gctggacgcc aacgtgctgt tcatgaccat catggccaac cgcatgccct tcgtcggccg
     2521 cggctagtca cttaagcggc gtgtgccagt cgaggtcatt cggcgatgaa atttgtcgcg
     2581 gctctgttcc ggtctcgata gcccggccgt cgatccatgc cgggtagcga ggtccgtcgg
     2641 cgtcgatcgc ggcgcggaag gcgaactgcg gttgcgcggc caggtcgcca tctgcgagca
     2701 gaacctcgaa acagcagtcg atgccgtcga gccgggcttg ccagtggtcc cggggctgcg
     2761 tggcgaacag ggccgcgagc tcgccgatca gcgcctgttg cggcatgctc tcgaattgcc
     2821 gcgctgtcca ttcgggtttt gtcacggcga tgcagaaatt ttcccagaac tgtt
//
LOCUS       DATCZV010000579         5778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_788783, whole genome shotgun sequence.
ACCESSION   DATCZV010000579 DATCZV010000000
VERSION     DATCZV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788783"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VIW27_13105"
     CDS             <1..2067
                     /locus_tag="VIW27_13105"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5740658.1"
                     /translation="KEETGAYCRSVAAAEFFDGDERFSQFLYSIGVVPNDLPNLFPFL
                     AREILHGHKLVVFPEGGMVKDKRVVDHRGRYSIFSRSANERRKHHRGSAVIALALDAF
                     KSAVLHDYSLGKYQRIERWAEQLGFDDLEEMMTRAVKPTVIVPSHITFYPIRVSDNIL
                     HQAARLFNRGINKRFAEELIIEGNLLFKDTDMDIRFAQPIVASQYWRWWEKRLLPSVV
                     HRFESLHELFALQPDPGHWGGRIHALGMKARSNKLRDDYMRRMYQAVTVNLSHIAALM
                     ILKLFENGFRRINRGRFHKMLYVAIKRLQESQHGLHRSLRNPEEYDAIINRGSIRLDQ
                     FLLSVQKMGLVHLEQNDYVLDDKLIADFEIDEIRTENLISVYANEISPLTEVTRIVGR
                     AMVRAEKLGARKIADYRFDDQLISYRWDHDRFQRERYREINSQQTQTADANCYFLKSK
                     RKAASAVLLVHGFMAGPAELRGLGERLHADGHHVLGVRLKGHGTSPWDLRERNWHDWA
                     NSVERGYDILRAYSREVHVVGFSTGGLLALHLAANNPGPRIRSVTSVSAPVHFRNKNM
                     IFVPLLHHANKLVSWVTSEGLLPFRPNKPENPELNYQHIPIRALYQLQQFIDFLTADT
                     VKINADVHLYQGDKDPVVDPESLRTLDRLIEAEDKKITWLRSDIHGVVYRNIDEVQQK
                     ICASLL"
     gene            2129..3391
                     /locus_tag="VIW27_13110"
     CDS             2129..3391
                     /locus_tag="VIW27_13110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5740659.1"
                     /translation="MHHPTRLLASGFAVVILMMIILAAFAFHSSGIADRQINVKVSRQ
                     LEKIDLISELSTIIQARTRFMQSMLLQDEQALSGKTPDHFNRLSGAYRETRERLLPLL
                     APREREIMNAIDRLDSDISDLNRQVSVLFLNGSRDEASKILLREVLPKTAPLLSHLTE
                     LTQAQRMDVQKALLLASGNAAENRLHIVVIAVFAVLISLTVTCLAIWYGRKLSGQLED
                     MNDYLEQKIYERTESLLDTQSKLLEDNSELTRMALTDSLTGLSNRTHMNQILYKEFAR
                     FERHNQRFGIIMLDIDHFKRINDQYGHDTGDTVLKKLARIFENSIRTSDFVARWGGEE
                     FLICCTTIEETDLLPIAETIRQLAASTEFEKVGPITASLGCAAIVKGETIGELIKRAD
                     IALYEAKNNGRNLTVVSEFTDLSIPTQD"
     gene            complement(3478..4032)
                     /locus_tag="VIW27_13115"
     CDS             complement(3478..4032)
                     /locus_tag="VIW27_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5740660.1"
                     /translation="MNYCSQCGQPVTLKIPVDDNRERFVCDACGYVHYQNPNNVCGAI
                     LHRDDEILLCRRAIEPRYGLWTLPAGFMENNETVAEAAAREAMEEANARAGPLALFGI
                     FSMPYISQVYLMFHGRLESEVAAGAESLEVGMFSVDRIPWDQLAFPVITHSLRLYFEQ
                     GTERVHHAWFSRRPDRSVEFHFVD"
     gene            complement(4029..4772)
                     /locus_tag="VIW27_13120"
     CDS             complement(4029..4772)
                     /locus_tag="VIW27_13120"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEY5740661.1"
                     /translation="MLAIIGGSGLYGLDEQFVAERQAARETPYGATSADIVQGCWQDL
                     PVAFLPRHGPDHDVPPHRINYRANIWALREIGASHIIAVNAVGGIAAELAPRALAVPD
                     QIIDYTSGREHTFCDGGDQQVRHVDFTWPYDAQLRSSLLEAGRQLGVPLVGSGTYGCS
                     NGPRLETAAEIARMRNDGCAMVGMTGMPEAALARELEIAYAGLALVVNWAAGIEQREI
                     TMEEIYGNLEQGMQQVKPLLRATAARVAQ"
     gene            complement(4772..5326)
                     /locus_tag="VIW27_13125"
     CDS             complement(4772..5326)
                     /locus_tag="VIW27_13125"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEY5740662.1"
                     /translation="MQAERAQQVHQTAELLFDQDAVEQAIADIAARVEADLGDKFPLV
                     LCVMNGGLYLAGQLLRHWEFPLTLDYVHATRYRLATLGKDVLWKAYPQNEIRDRNVII
                     IDDIFDQGYTLEEITAYCMKHGARQCHSVFLMRKTHERKICDARADYHGLECGDCYVY
                     GAGMDLNGHFRNLSSIYSLPEKVV"
     gene            complement(5329..>5778)
                     /locus_tag="VIW27_13130"
     CDS             complement(5329..>5778)
                     /locus_tag="VIW27_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5740663.1"
                     /translation="GTVEKIGWRLTVIRDFQSRPIYVPNSIFTNIIVENPSRMSNRRI
                     YETIGLRYSDLTSMDKIVREVEDMLRAHPEIDADKTLMVNFNAFADSSADFFIYCFTK
                     TTQWVKFHGVKQDVMLRIAAIIEANHAEIAFPTSTIHLADSVSINQA"
BASE COUNT         1226 a         1807 c         1643 g         1102 t
ORIGIN      
        1 aaggaggaaa ccggcgccta ttgccgctcg gtcgcggcgg cggaattctt cgacggcgac
       61 gagcgcttca gccagtttct gtactcgatc ggcgtggtcc ccaacgattt accgaacctg
      121 tttccgttcc tggcgcggga aatcctgcac gggcacaagc tggtggtgtt tcccgagggc
      181 ggcatggtca aggacaagcg ggtagtcgat catcgcgggc gctacagcat tttttcccga
      241 tccgccaacg agcgccgcaa acaccaccgc ggctcggcgg tgatcgcgct ggcgctggac
      301 gccttcaaga gcgccgtgct gcacgattac tcgctcggca agtaccagcg catcgaacgc
      361 tgggcggaac aactcggctt cgacgatctc gaggaaatga tgaccagggc ggtcaagccc
      421 acggtgatcg tgccgagcca catcaccttc tacccgattc gcgtcagtga caacattctt
      481 catcaggctg cacgcctgtt caaccgcggc atcaacaagc gcttcgccga ggagctgatc
      541 atcgagggta acctgctgtt caaggatacc gacatggata ttcgcttcgc gcagccaatc
      601 gtcgccagcc agtactggcg ctggtgggaa aagcgcctgc tgccaagtgt cgtgcaccgt
      661 ttcgaatcgc tgcacgagct atttgcgcta caacccgatc ccggccactg gggcgggcgc
      721 attcacgcgc tcggcatgaa agccaggtcg aacaagctac gcgacgacta catgcgccgc
      781 atgtatcagg cggtgaccgt caatctcagc catatcgccg cgctgatgat actgaaactg
      841 ttcgagaacg ggtttcgccg catcaaccgc ggccgctttc acaagatgct ctacgttgcc
      901 atcaagcgct tgcaggaatc gcagcacggc ctgcaccgca gcctgcgtaa tcccgaagag
      961 tacgacgcga tcatcaacag gggcagcatt cgcctcgacc agttcctgct cagcgtgcag
     1021 aaaatgggcc tggtgcacct ggagcagaac gattacgtac tcgacgacaa attgatcgcc
     1081 gatttcgaga tcgacgaaat ccgcaccgaa aacctgatca gcgtctatgc caacgagatc
     1141 agcccattaa ccgaagtcac ccgcatcgtc gggcgggcaa tggtgagggc cgaaaagctc
     1201 ggcgcgcgaa aaatcgccga ttaccgcttc gacgaccagc tgatttccta ccgctgggat
     1261 cacgaccgct tccagcgcga acgctaccgg gaaatcaatt cccagcaaac ccagaccgcc
     1321 gatgccaact gttatttcct gaagtcgaag cgcaaggcgg ccagcgcggt gctgctggtg
     1381 cacggtttca tggccggccc ggccgagttg cgcggcctcg gcgagcgcct gcatgccgac
     1441 ggccaccacg tgctcggcgt gcggctcaag ggacacggca cttcgccgtg ggatttgcgc
     1501 gaacgcaact ggcacgactg ggcgaattcg gtcgagcgcg gttacgatat cctcagggcc
     1561 tacagccgcg aggttcacgt ggtcggcttt tccaccggcg gcctgctggc cctgcacctg
     1621 gcggcgaata acccgggacc gcgtatcagg tcggtcacca gcgtctcggc accggtgcat
     1681 tttcgcaaca agaacatgat cttcgtgcct ctgttgcacc atgccaacaa gctggtcagc
     1741 tgggtgacct ccgagggatt gctgccgttt cggcccaata aaccggagaa cccggagctc
     1801 aactaccagc atatcccgat tcgggcgctt taccagctgc agcaatttat cgacttttta
     1861 accgcagata ccgtcaagat aaacgcagat gtccaccttt accagggaga taaagatccc
     1921 gtcgtggatc ccgagagcct gcgcacactc gacaggctga tcgaggcaga ggacaagaaa
     1981 atcacctggc tacgcagcga tatccacggt gtcgtctacc gcaatatcga cgaggtacaa
     2041 cagaaaattt gcgcttctct cctataatca gttaaatatc agtccctgca tttgggaggg
     2101 acgaaggtcc agactaactc tctaattcat gcaccatcct acccgcctgc tagcctccgg
     2161 cttcgctgtc gttattctga tgatgatcat cctggccgcg ttcgccttcc attcgagcgg
     2221 aatcgccgat cgccagatca acgtcaaggt ttcgagacag ctggaaaaaa tcgacctgat
     2281 cagcgaattg tcgacgatca tccaggcccg tacacgcttc atgcaatcca tgctgctgca
     2341 ggacgagcag gcgttatccg gcaaaacgcc cgatcatttc aaccgcctga gcggcgccta
     2401 ccgcgaaacc cgcgaacgcc tgctaccgct gctggcaccg cgcgaacgcg aaatcatgaa
     2461 tgccatcgat cgcctcgaca gcgatatcag cgacctcaac cggcaggtct cggtgctgtt
     2521 tctcaacggc agtcgcgacg aggccagcaa gatactgctg cgcgaagtgc tgccgaaaac
     2581 cgcgccgctg ctgtcgcacc tgacggaact gacccaggca cagcgcatgg acgtgcagaa
     2641 agcgctgctg ctggccagcg gcaatgccgc ggagaaccgg ctgcacatcg tggtcatcgc
     2701 cgtattcgcg gtgctcatca gcctcacggt gacctgcctc gcgatctggt acgggcgcaa
     2761 gttgtcgggc caactcgagg atatgaacga ttacctcgag caaaagatct acgagcgtac
     2821 cgagtcgctg ctcgataccc agagcaagtt gctcgaggac aatagcgaac tgacccgcat
     2881 ggcgctgacc gacagcctga ccgggctgtc caaccgtact cacatgaacc agatcctgta
     2941 caaggaattc gcgcgcttcg agcgccacaa ccagcgtttc ggcatcatca tgctcgatat
     3001 cgatcacttc aagcgtatca acgatcagta cggccacgac accggcgaca cggtactgaa
     3061 aaagctcgcg cggattttcg agaattcgat tcgcaccagc gacttcgtcg cccgctgggg
     3121 cggcgaggaa ttcctgatct gctgcacaac catcgaggaa accgatttgc tgccgatcgc
     3181 cgaaaccatt cgccagttgg ccgccagcac cgaattcgaa aaagtcggcc cgattaccgc
     3241 cagcctgggc tgcgcggcga tagtcaaggg ggagaccatc ggcgaattga tcaagcgtgc
     3301 cgatatcgcg ctctacgagg ccaagaataa cggccgcaac ctgaccgtgg tctccgaatt
     3361 caccgatctg tcgataccca cccaggattg aaaccggccg cggcgcggca accaagcccg
     3421 taatcgtcaa tgccgtcgct cgccggggca gcgcgacaaa tcgccgacga atagcggcta
     3481 atcgacgaaa tggaattcga cgctacggtc cggccgacgg ctgaaccagg cgtgatgcac
     3541 ccgctcggta ccctgctcga aatacagcct taggctgtgc gtaatcaccg ggaatgccaa
     3601 ctgatcccag ggaattcgat cgacgctgaa catgccgacc tcgagactct cggctccggc
     3661 ggcaacctcg ctctcgagtc gcccgtgaaa catcaggtaa acctgcgata tatagggcat
     3721 gctgaagatg ccgaacagcg ccagcgggcc ggcgcgcgca ttggcttctt ccatggcttc
     3781 gcgggcagcc gcctcggcca cggtctcgtt gttttccatg aatcccgcgg gcagcgtcca
     3841 tagcccgtaa cgcggttcga tcgcgcgccg gcacagcagg atttcatcgt cacggtgaag
     3901 aatcgcgccg cagacgttgt tgggattctg gtaatgcacg tagccgcagg cgtcacagac
     3961 gaaacgctcg cggttatcat cgaccggtat tttcagagtc accggctgcc cgcactggct
     4021 gcagtagttc attgcgccac ccgtgctgcg gttgcccgca acaggggctt cacctgctgc
     4081 atgccctgct cgaggttgcc gtaaatctct tccatggtga tctcccgctg ctcgattccg
     4141 gccgcccagt tgaccaccag cgccagtccg gcataggcga tctcgagttc acgcgccagc
     4201 gccgcttccg gcatgccggt catgcccacc atcgcacagc cgtcgttgcg catgcgggca
     4261 atctcggccg cggtctcgag ccgcggcccg ttactgcaac cgtaggttcc cgacccgacc
     4321 agcggcacgc cgagttgcct gccggcctcg agcaggctgc tccgcaattg tgcatcgtag
     4381 ggccaggtga agtcgacatg ccgaacctgc tggtcgccgc cgtcgcagaa agtgtgttct
     4441 cgaccggagg tgtaatcgat gatctggtcg ggcaccgcca gcgcccgcgg cgccagctcg
     4501 gcggcgattc cgccgaccgc gttaaccgcg atgatatggc tggcgccgat ctcccgcagc
     4561 gcccagatat tggcccggta gttgatacgg tgcggcggca cgtcgtgatc cgggccgtgg
     4621 cgcggcagaa aagccactgg caaatcttgc cagcaaccct gcacgatatc cgccgacgtc
     4681 gctccgtacg gcgtttcgcg cgccgcctgc cgctcggcga caaactgttc atccaggcca
     4741 tagaggccgc tgccgccgat gatcgccagc atcacacgac cttttcgggc agtgaataga
     4801 tactcgacag attgcgaaaa tgcccgttca gatccatgcc ggcaccgtac acatagcaat
     4861 caccgcactc caggccatga tagtcggcgc gcgcgtcgca gatcttgcgt tcgtgggtct
     4921 tgcgcatcag gaacacgctg tgacattgcc tggcgccatg cttcatgcaa taggcagtga
     4981 tttcctcgag cgtgtatccc tggtcgaaaa tatcgtcgat gatgatgacg ttgcgatccc
     5041 tgatctcgtt ttgcggataa gccttccaca acacgtcctt gcccagcgtc gccagccggt
     5101 agcgggttgc atggacataa tccagggtaa gcggaaactc ccagtgccgc agcagctgtc
     5161 cggcgaggta caaaccgccg ttcatgacac acaggaccaa tggaaacttg tcgccaaggt
     5221 cggcttcgac gcgggcggcg atatcggcta tcgcctgctc gacggcatcc tggtcgaaca
     5281 ataactcggc ggtttggtgg acttgctggg cgcgctcggc ttgcatggct aggcctgatt
     5341 gatggacacg gaatcggcca ggtgaatcgt cgaagtcgga aacgcgatct cggcatggtt
     5401 ggcctcgatg atggcggcga tgcgcaacat gacgtcctgc ttgaccccgt ggaattttac
     5461 ccactgcgtg gttttcgtaa agcagtagat gaaaaagtct gccgaggaat cggcaaacgc
     5521 gttgaaattc accatcagcg tcttgtcggc atcgatctcg ggatgcgccc tgagcatatc
     5581 ctcgacctcg cggacgatct tgtccatgct ggtcaaatcg gaataacgca gaccgatggt
     5641 ttcgtagatg cgccggttcg acatccgcga cggattttcg acgatgatgt tggtgaagat
     5701 cgagttgggc acgtagatcg gccgcgactg gaaatcgcga atcacggtca gccgccagcc
     5761 gatcttttcg accgtacc
//
LOCUS       DATCZV010000580         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_206178, whole genome shotgun sequence.
ACCESSION   DATCZV010000580 DATCZV010000000
VERSION     DATCZV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206178"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VIW27_13135"
     CDS             complement(<1..527)
                     /locus_tag="VIW27_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5740664.1"
                     /translation="MSTESAQPAGEAAIRISNVWKIFGERADEALQAIREQGLGKSEV
                     LETYNAVVGVADVSLEVNKGEIFCIMGLSGSGKSTLVRHFNRLLEPTDGKIEIHGTDV
                     MALDPAGLMEFRNRKIGMVFQNFALLPHRSVLDNVAMPLEIRNINKNERMRRAANMLK
                     TVELDAWGNKYAHELS"
     gene            complement(618..1580)
                     /locus_tag="VIW27_13140"
     CDS             complement(618..1580)
                     /locus_tag="VIW27_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012178110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5740665.1"
                     /translation="MKKLISSLILVTGMALSTAALSAEKVNIGVPAWTGAQAIAALLQ
                     AVVEERIGGEANLVPGNNATIFQGMDQGKGDIDVHPDVWLPNQESFTKKYVDGAGTVE
                     LSKNPYEGNQGFCVSQDFAKKKNITDIADLGRPDVAMEMDSDGNGKGEMWIGAPGWAS
                     ANVNEVKVRDYQLLSFIEPIRAEESVKTARVKDSIAKSEGYAFYCYKPHAIWFMFDVT
                     MLTEPKFDPAKYVMVQPSDSPDWYKESKVHTKDALKKVQIAWSKSLRERSPAIVEFFE
                     RFSLTADDVSNFAFEVSGKGRDASEVAREWIAANPERVDSWLGL"
     gene            complement(1640..>2206)
                     /locus_tag="VIW27_13145"
     CDS             complement(1640..>2206)
                     /locus_tag="VIW27_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012178111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5740666.1"
                     /translation="IPGGGSNEIGALGYVVCAQEIVEQSADLGVEFDWIVMGTGSTGT
                     QAGLVAGLHLLDRQLPVMGISVRQPRDRQVRAVHDLAARTLEYIDGGTLSANTVVVDD
                     DYVGEGYGMPAPSTIEAIEMAARYEGLLLDPVYSGKGFAGLIGLARANFFKPTDKVLF
                     LHTGGSAALFAYEDALFAKDDWLAGADS"
BASE COUNT          396 a          725 c          632 g          453 t
ORIGIN      
        1 gacagttcgt gtgcgtactt gttgccccag gcgtcgagtt ccaccgtctt cagcatgttg
       61 gcggcgcggc gcatgcgctc gttcttgttg atattgcgaa tctcgagcgg catcgccacg
      121 ttgtcgagca ccgagcggtg cggcagcaat gcgaaattct gaaacaccat gccgatcttg
      181 cggttgcgaa actccatcag gccggcggga tcgagcgcca tcacgtcggt gccgtgaatc
      241 tcgatcttgc cgtcggtggg ttccagcagg cggttgaagt ggcgcaccag ggtcgacttg
      301 ccgctgcccg acaggcccat gatgcagaag atctcgccct tgttgacctc gaggctgacg
      361 tcggcgacgc cgaccaccgc attgtaggtt tccagcacct cggacttgcc cagaccctgc
      421 tcacgaatcg cctgcagtgc ttcgtcggcc cgctcgccga aaatcttcca gacgttggaa
      481 atgcggatgg cggcttcgcc cgccggctgt gcactctcgg tagacacgat cgctcccgtc
      541 gattgcgcga aagggttgcc cgccgaaccg ctgcctcagc tgcggcggtc cggcaggcca
      601 aggaggaaat tacaggttca caatcccagc caggaatcga cacgctcggg attggcggcg
      661 atccattcgc gcgccacctc ggacgcgtcg cggcctttgc cggaaacctc gaaggcgaag
      721 ttgctgacgt catcggcggt tagcgaaaag cgctcgaaga attcgacgat ggccggtgaa
      781 cgctcgcgca gcgacttcga ccaggcgatc tgtaccttct tcagcgcatc cttggtatgc
      841 accttcgatt ccttgtacca gtcaggcgaa tcggagggct gcaccatcac gtacttggcc
      901 ggatcgaact tcggctcggt gagcatggtg acatcgaaca taaaccagat cgcgtgcggc
      961 ttgtagcaat agaacgcgta gccttcactc ttggcgatcg aatccttgac acgcgcggtc
     1021 ttgacgcttt cctcggcgcg aatcggctcg atgaaggaca gcagctggta atcgcgcacc
     1081 ttgacctcgt tgacgttggc cgaggcccag cccggcgcgc cgatccacat ctcgcccttg
     1141 ccattgccgt cgctgtccat ctccatcgcg acatccgggc gaccgagatc cgcgatatcg
     1201 gtgatattct ttttcttcgc aaaatcctgg gacacgcaaa aaccctggtt gccctcgtaa
     1261 ggattcttgc tcagttccac ggtgcctgca ccgtcgacgt atttcttggt gaaactttcc
     1321 tggttcggca accagacgtc gggatgcacg tcgatgtcgc ccttgccctg gtccatgccc
     1381 tggaagatgg tcgcgttgtt gcccggcacc aggttggcct caccgccgat gcgctcttcg
     1441 accacggcct gcaacagcgc cgctatcgcc tgcgcgccgg tccaggccgg tacgccgatg
     1501 ttgacttttt ccgcgctcaa cgcggccgtc gacaaagcca ttcccgtcac caggatcaag
     1561 ctcgatatca atttcttcat agttcgactc ctcggtcttc gtttatgcca atagttttat
     1621 tttcagcggc ccggacgagt tacgaatccg ccccggccag ccaatcgtcc tttgcgaata
     1681 gtgcgtcctc gtaggcgaac aatgccgccg aaccgccggt atgcaggaac agcaccttgt
     1741 ccgtcggttt gaaaaagttg gctcgcgcca gcccgatcag gccggcgaac cccttgcccg
     1801 agtaaaccgg atcgagcaac agcccctcgt atctcgccgc catctcgatg gcctcgatcg
     1861 tcgacggtgc cggcatgccg taaccctcgc cgacatagtc atcgtcgacg accaccgtgt
     1921 ttgccgacag cgtgccgcca tcgatgtact cgagggtgcg cgcggccagg tcgtgaaccg
     1981 ccctgacctg ccggtcgcgc ggctggcgca cgctgatccc catcaccggc agttgcctgt
     2041 cgagcaaatg caacccggcg accagccccg cctgggtgcc ggtactgccg gtgcccatga
     2101 cgatccagtc gaactcgacg ccgaggtcgg cgctctgctc gacgatctcc tgcgcgcaga
     2161 ccacgtaacc gagcgcgccg atctcgttgg agccgccgcc gggaat
//
LOCUS       DATCZV010000581         6471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_152871, whole genome shotgun sequence.
ACCESSION   DATCZV010000581 DATCZV010000000
VERSION     DATCZV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152871"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /gene="mrdA"
                     /locus_tag="VIW27_13150"
     CDS             <1..1052
                     /gene="mrdA"
                     /locus_tag="VIW27_13150"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5740667.1"
                     /translation="GAAVAVDPNNGEVLALVSMPVFDPNLFVNGISYANYNQLRDSPD
                     RPLFNRALSGQYPPGSTTKPFFAFAGLELGAVTAEHKVFCPGYYQLPNEPRKYRDWKK
                     EGHGHMDLDGAVTQSCDVYFYKLAYELTIDRMAAFMQQFGFGRKTGIDSTGERSGLMP
                     TREWKRGAEGLPWFPGETLITGIGQGALLVTPLQLANSTAAFASWGTRFQPRLVKAVE
                     KIPQYEKVATSSSIAGRYEVVKPENWEHVHQAMVNVVHGLRGTAHRISRGLDYQVAGK
                     TGTAQVFGIAQDEEYDEEEVQEKLRDHALFVSYAPADAPRIVVAVIVENGGHGSSVAA
                     PIARLIMDAYLLEDT"
     gene            1097..2170
                     /gene="rodA"
                     /locus_tag="VIW27_13155"
     CDS             1097..2170
                     /gene="rodA"
                     /locus_tag="VIW27_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018994764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY5740668.1"
                     /translation="MDALRLDPVLLSLLLMLAAFGSMVLYSASGADEAAMIRQTTRIL
                     VAMLLLIATANLPLRVIRKMSVWLYLGGIALLVAVILFGEVGKGAQRWLDLGFIRFQP
                     SEILKLAVPMVIAAYLSNRVLPVGFKDLVVSTVLVLIPVVLIARQPDLGTAILVGSAG
                     FFVLFLAGVRWSVIVATGVLLSAAAPLMWKFVLHEYQRDRILTLLDPESDPLGSGYHI
                     IQSKIAIGSGGVYGKGWLNGTQSQLDFIPERSTDFIFSVFGEEFGFLGSVILILLYLA
                     IIFRGLYIAAHAEENFGRLLAGALSLTFFVYLFVNTGMVAGILPVVGVPLPLVSYGGT
                     SMVTLMVGFGLIMSIRSAKRFLV"
     gene            2170..3168
                     /gene="mltB"
                     /locus_tag="VIW27_13160"
     CDS             2170..3168
                     /gene="mltB"
                     /locus_tag="VIW27_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY5740669.1"
                     /translation="MKKFTILLIAVLAAPLAGAVELTGGDYQGRDDVGAFVKRMVDET
                     DYTERELVELFSSVTRQEHLFAQLDRPAEKELDWYQYRPIFIKDRRVELGVEFWREHR
                     ELLTRVSEQSGVPPEIIVAIIGVETFFGIYKGKDPVFDSLVTLAFDYPRRAPFFTREL
                     REFLLLVKEQGFDARALRGSYAGAMGMPQFISSSYRAYAVDFDGDGQTNLFDSIADIT
                     GSVANYFVKHGWQRGGRVARPLDIGDARSISMLQPGVKPEYKWADLKRNGLSSQFEIG
                     DETPVALVELKQRDGSEYWAGFQNFYVITRYNHSELYAMAVYQLSKLISHQYKKTA"
     gene            3165..3941
                     /locus_tag="VIW27_13165"
     CDS             3165..3941
                     /locus_tag="VIW27_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY5740670.1"
                     /translation="MTVSRLTSVLLPLLLCACTFGVPIGERGAQQGKPTSAKSSRGNP
                     PSYVVHGKRYYVMDSSEGFVQRGIASWYGRKFHGRKTSSGEVYNMHDMTAAHKTLPIP
                     VYVHVRNLDNGRTAIVRVNDRGPFIDGRIIDLSYAAATRLGVDGPGTANVEISVIERG
                     QTQPTSVVRAVPLATPESRDQPLYIQMGSFASLNNADNLVRTLLAANESAAQVSPLQT
                     DDGLFYRVRVGPLFDIDEANAILARLRGKGFESIRIVVED"
     gene            4018..5157
                     /locus_tag="VIW27_13170"
     CDS             4018..5157
                     /locus_tag="VIW27_13170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY5740671.1"
                     /translation="MKIVSLVTGVLALLLVATGWAASRPIPNPPELNASSYLLIDFDS
                     GVTLAEKDADKPVDPASITKLMTAYLIDKAIADGTIASDDPVTISEKAWRMEGSKMFV
                     EVGKQVSVENLLKGLIIQSGNDSSVALAEHLGGSEEAFVGYMNHQARLLGMSNTNFTN
                     STGWPDENHYSTARDIAILTRAVIRDFPQSYRLYKEREYTFNDIRQFNRNRLLWRDDS
                     VDGVKTGHTEAAGYCLVASAIREDMRLISVVLGSDSDKSRTQSSQALLNYGFRFFETH
                     RLYTADEVLNRARIWYGDQQEVSMGVGRDIHITIPRGRYRDLQASMEVDAEISAPVVR
                     GQQLGLVNIRLDDELLLSEDLVAMQDVGEGSLIDQAMDRIKLMFK"
     gene            5157..5444
                     /locus_tag="VIW27_13175"
     CDS             5157..5444
                     /locus_tag="VIW27_13175"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEY5740672.1"
                     /translation="MAAEFPGGGNGLALIDYPSRFPLKVFGNDEPGFETTVLELIRGR
                     CPQAEHIEVRRRRSRGGKYLALTLTFTVYSQQQLEQIYQDLYDCERVVMSL"
     gene            5461..6093
                     /gene="lipB"
                     /locus_tag="VIW27_13180"
     CDS             5461..6093
                     /gene="lipB"
                     /locus_tag="VIW27_13180"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY5740673.1"
                     /translation="MLVRKMGLSDYRETWMAMRRFTDERDTGTEDELWLTEHPPVFTQ
                     GLNGRAEHLLAPGDIPVVQVDRGGQVTYHGPGQLVMYCLLDIVRLGVGVKGLVGRIEE
                     SIVALLEAYGIEAHARAGAPGVYVGEAKIAALGLRIRKGCCYHGLSLNVDLDLEPFDR
                     INPCGFRGLAVTQLRDLGVGDDLQTIGDRLAGILKERLSEKKPGSTGRQA"
     gene            6056..>6471
                     /locus_tag="VIW27_13185"
     CDS             6056..>6471
                     /locus_tag="VIW27_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5740674.1"
                     /translation="MKKNQVVQGVKLKGADKVARIPIKVVPSKAVARKPAWIRARAPV
                     GNRVKQLKARLRENDLYTVCEEASCPNLGECFSKGTATFMIMGDICTRRCPFCDVGHG
                     RPNPLDSEEPAKLARTISGMGLRYVVITSVDRDDLR"
BASE COUNT         1222 a         1938 c         2032 g         1279 t
ORIGIN      
        1 ccggtgccgc ggtggcggtc gatcccaaca acggcgaagt cctggcgctg gtgtcgatgc
       61 cggtattcga cccgaacctg ttcgtcaacg gcatcagtta cgcgaattac aaccagttgc
      121 gcgactcgcc ggaccggccg ctgttcaacc gcgcgctatc gggacaatat ccgccgggat
      181 cgacgaccaa gccctttttt gccttcgccg ggctcgagct gggtgccgtc accgccgaac
      241 acaaggtctt ctgcccgggt tactaccagt tgccgaacga gccgcgcaag taccgtgact
      301 ggaagaagga gggccatggg cacatggacc tggacggcgc tgtcacccag tcctgcgacg
      361 tgtattttta caagctggcc tacgagctga cgatcgatcg catggcggca ttcatgcagc
      421 agttcggctt cggccgcaag accggcatcg acagcaccgg cgagcgcagc ggcctgatgc
      481 cgacgcgcga atggaagcgc ggcgccgagg gcctgccgtg gttcccgggc gagaccctga
      541 ttaccggtat cggccagggc gctttgctgg tcacgccgct gcaactcgcc aactcgaccg
      601 ccgccttcgc gtcctgggga acgcgctttc agccgcgact ggtgaaggcg gtggaaaaga
      661 taccgcaata cgaaaaggtc gctaccagca gcagcatcgc cggccgctac gaggtcgtca
      721 agccggagaa ctgggaacat gtgcaccagg caatggtcaa tgtcgtgcac ggcttgcgcg
      781 gtaccgcgca ccgcatctcc cgcgggctcg attaccaggt cgccggcaag accggcaccg
      841 cgcaggtgtt cgggattgcc caggacgagg aatacgacga ggaagaggtg caagaaaaac
      901 tgcgcgacca cgccttgttc gtcagttacg cgccggccga cgcgccgcgc atcgtggtcg
      961 cggtaatcgt cgagaatggc gggcacggta gctcggtagc ggcgccgatc gcgcgcctca
     1021 tcatggatgc ctacctgctg gaggatactt gaaccagacg ctggagttcg atacgccctc
     1081 ggtttcgcgg cgggtaatgg acgcgctgcg cctcgatccg gtgctgttgt cgttgctgct
     1141 gatgctggcg gccttcggta gcatggtcct gtacagcgcc agcggtgccg acgaagcggc
     1201 gatgatacgc cagaccaccc gcatactggt ggcgatgctg ctgctgatcg cgaccgccaa
     1261 cctgccgctg cgcgtgattc gcaaaatgtc ggtctggctt tatctcggcg gtatcgcgct
     1321 gctggtcgcg gtcatcctgt tcggcgaggt cggcaagggt gcgcagcgct ggctggacct
     1381 cggtttcatc cgttttcagc cttccgagat tctcaagctc gcggtgccga tggtgatcgc
     1441 cgcctattta tccaaccgcg tgttgccggt cggcttcaag gacctggtgg tatcgacggt
     1501 gctggtgctg attccggtgg tgctgatcgc gcgccagccc gatctcggta ccgcgatcct
     1561 ggtcggcagc gccggcttct tcgtgctttt cctcgccggt gtgcgctgga gcgtgatcgt
     1621 cgccaccggc gtcctgctgt cggcggcggc gccgttgatg tggaagttcg tgttacacga
     1681 gtatcagcgc gatcgtattc tcactctgct tgatcccgaa agcgatccgc tgggctcggg
     1741 ctaccatatc atccagtcga aaatcgccat cggctcgggc ggagtttacg gcaagggctg
     1801 gctgaacggc acccagtcgc agctggattt cattcccgag cgttccaccg atttcatctt
     1861 ttcggtattc ggcgaagaat tcggttttct cggcagcgtg atattgatct tgttgtatct
     1921 ggcgattatc tttcgcggtt tgtatatcgc cgcgcatgcc gaggaaaatt tcggtcgcct
     1981 gctggccggt gccctgagcc tgacgttttt tgtctacctg ttcgtcaaca ccggcatggt
     2041 cgccggcatc ctgccggtgg tcggggtacc cttgccgctg gttagctacg gcggcacgtc
     2101 gatggtaacg ctgatggtgg gattcggcct gatcatgtcg atccgctcgg caaaacggtt
     2161 tttggtttga tgaagaaatt tacgatattg ctgatcgccg tgctggccgc gccgctggcg
     2221 ggggcggtag agctcaccgg cggtgattat cagggccgcg acgacgtcgg cgctttcgtc
     2281 aagcgcatgg tcgacgagac cgattacacc gagcgcgaac tcgtcgaact gttttccagc
     2341 gtcaccaggc aggaacacct gttcgcgcag ttggaccgcc cggcggagaa ggaactcgac
     2401 tggtaccaat accgcccgat attcatcaag gaccggcgtg tcgagctcgg cgtggagttc
     2461 tggcgcgagc atcgcgaatt gctgaccagg gttagcgaac aatccggcgt gccaccggaa
     2521 atcatcgtcg cgatcatcgg cgtggaaacc tttttcggca tctataaggg caaggacccg
     2581 gttttcgaca gcctggtcac gctggccttc gattatccgc gccgtgcgcc gtttttcacc
     2641 agagaactca gggaattcct gctgctggtc aaggagcagg gtttcgatgc gcgcgccctg
     2701 cggggttcct atgccggcgc catgggtatg ccgcaattca tctcttcgag ttatcgcgcc
     2761 tatgcggtcg atttcgacgg cgacggtcag accaacctgt tcgactcgat cgccgatatc
     2821 accggcagcg tcgccaatta cttcgtcaag cacggctggc aacgcggcgg ccgggtggcc
     2881 aggccgctgg acattggtga cgcccgctcg atctccatgc tgcagcccgg cgtcaaaccg
     2941 gagtacaagt gggccgacct gaagcgcaat ggattgtctt cacaattcga gatcggcgac
     3001 gagacgccgg tggcgctggt cgaactcaag cagcgtgacg gttccgagta ctgggcgggt
     3061 ttccagaact tttacgtgat cacgcggtat aatcacagcg aactctacgc gatggcggtt
     3121 tatcagttgt ccaagttgat cagccaccag tacaagaaaa cagcatgacg gtctctcgcc
     3181 tgacctctgt gctgctgccg ttgctgcttt gcgcctgtac ctttggtgtg ccgattggcg
     3241 aacgcggcgc gcagcagggc aaaccgacct cggccaaatc cagccgcggc aatcctccat
     3301 cctacgtcgt tcacggcaag cgctactatg tcatggacag ttccgagggt ttcgtgcaac
     3361 gcggcatcgc ctcatggtac ggccgcaagt ttcatggccg taaaacctcg agtggcgagg
     3421 tctacaacat gcacgacatg accgcggcgc acaaaaccct gccgatcccg gtatacgtgc
     3481 acgtcaggaa tctcgataac gggcgaaccg cgatcgtgcg cgtcaatgat cgcgggccgt
     3541 tcatcgacgg gcgcatcatc gatttatcct atgccgccgc gacccgcctc ggcgtcgatg
     3601 ggccgggaac cgccaatgtc gagatcagcg tcatcgagcg cgggcagacg caaccgacca
     3661 gcgtggtgcg tgcggtaccg ctggcaaccc cggaatcgcg cgaccagccg ttgtacatcc
     3721 agatgggttc gtttgccagc ctcaacaatg ccgacaacct ggtcagaacc ctgctggccg
     3781 ccaacgagtc ggcggcccag gtctcgccgc tgcagaccga cgacggcctg ttttaccggg
     3841 ttcgcgtcgg cccgctgttc gacatcgacg aggccaacgc gatcctcgcg cggctcaggg
     3901 gcaagggatt cgaatcgatc cgcatcgtcg tcgaggattg agccgatagc ccttgagggt
     3961 gctggcggtg accgctacaa tcgcgagtct tcatcgctca ctactcctca ggtcattatg
     4021 aaaatcgttt cgctggtaac cggagtcctg gccctgttgc ttgtcgcgac cggctgggcc
     4081 gcatccaggc cgataccgaa cccgcccgag ctcaacgcca gcagttatct gctgatcgat
     4141 ttcgattcgg gggtaacgct tgccgagaag gatgccgaca agccggtaga tcccgccagt
     4201 attaccaagt tgatgaccgc ctacctgatc gacaaggcga tcgccgacgg cacaatcgcc
     4261 agcgacgacc cggtcaccat cagtgagaaa gcctggcgca tggaggggtc gaaaatgttc
     4321 gtcgaggtcg gcaagcaggt atcggtcgag aatttgctga agggcttgat catccagtcc
     4381 ggcaacgatt cgagcgtggc gctggccgag cacctggggg gatcggaaga ggcctttgtc
     4441 ggctatatga accaccaggc acgcctgttg ggcatgagta acacgaattt caccaactcc
     4501 accggctggc cggacgaaaa tcattacagc acggcgcgcg acatcgcgat cctgacgcgc
     4561 gcggtaatca gggacttccc gcaatcctac cgtctttaca aggagcgcga gtacaccttc
     4621 aacgatattc gtcagttcaa ccgcaaccgc ctgctgtggc gcgacgacag tgtcgacggc
     4681 gtaaaaaccg gccataccga ggccgccgga tactgcctgg tggcctcggc gattcgcgag
     4741 gatatgcgcc tgatctcggt ggtattggga tcggattccg acaagtcgcg cacacagagc
     4801 agccaggcgc tgctcaacta cggtttccgc tttttcgaaa cccatcgcct gtacaccgcc
     4861 gacgaggtac tgaaccgcgc gcgcatctgg tacggcgatc agcaagaggt cagcatggga
     4921 gtcgggcgcg acatccatat caccattccg cgcggccgtt accgcgatct gcaggcgtcg
     4981 atggaagtcg atgccgagat cagcgcgccg gtggtgcgcg gccagcaact ggggctggtg
     5041 aacattcggc tcgacgacga gctgctgttg agcgaagacc tggtcgcgat gcaggatgtc
     5101 ggcgagggca gcctgatcga tcaggcgatg gaccgaatca agctgatgtt caagtaatgg
     5161 ccgcagaatt ccccggcggc ggcaatggcc tggcgttgat cgattacccg agcaggttcc
     5221 cgctcaaggt gttcggcaac gatgaaccgg gattcgaaac cacggtgctc gagctgatac
     5281 gagggcgctg tccgcaggcg gagcatatcg aggtgcgccg ccgacgtagc cggggcggca
     5341 agtacctggc gctgaccctg acctttaccg tctacagcca gcagcagctg gagcaaatct
     5401 accaggacct gtatgactgc gagcgggtcg tgatgtctct ttaacagggg gtgtcgcgac
     5461 atgctggtga ggaagatggg attgtccgac taccgcgaga cctggatggc gatgcggcgg
     5521 tttaccgacg agcgcgatac cggtaccgaa gacgagctgt ggctgaccga gcacccgccg
     5581 gttttcaccc agggtttgaa tggccgcgcc gagcacctgc tggcaccggg cgacattccc
     5641 gtggtgcagg tcgatcgcgg cggccaggtc acctatcacg gtcccggcca gctggtgatg
     5701 tattgcctgc tcgacatcgt gcgcctcggt gttggcgtca agggcctggt cggacgcatc
     5761 gaggagtcga tcgtcgcact gctcgaggct tacggcatcg aagcgcacgc gcgtgctggc
     5821 gcgccagggg tctatgtcgg cgaggccaag atcgcggccc tcggattgcg tattcgaaaa
     5881 ggatgttgtt atcatggcct gagtctgaat gtcgacctcg atctcgaacc cttcgaccga
     5941 atcaatccct gcggatttcg agggctcgcg gtcacgcaac tgcgcgatct gggcgtcggc
     6001 gacgatctgc aaacgatcgg cgacaggctg gcaggcatac tgaaggaacg tttgagtgaa
     6061 aaaaaaccag gtagtacagg gcgtcaagct taagggcgcc gacaaggtcg cgcgcattcc
     6121 gatcaaggtc gtgccgtcga aggccgtggc gcgcaaaccc gcctggatac gcgcccgcgc
     6181 gccggttggc aaccgtgtca agcaattgaa ggcgcggttg cgcgaaaacg acctgtacac
     6241 ggtttgtgag gaagcttcct gccccaatct cggcgaatgt ttttccaagg gtacggcgac
     6301 cttcatgatc atgggcgata tctgtacccg ccgttgcccg ttttgcgacg ttggccatgg
     6361 ccgcccgaac cccctggata gcgaagagcc ggcaaagctc gcacgcacca tcagcggcat
     6421 ggggctgcgc tacgtggtca ttacctcggt cgaccgcgac gacctgcgcg a
//
LOCUS       DATCZV010000582         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_99833, whole genome shotgun sequence.
ACCESSION   DATCZV010000582 DATCZV010000000
VERSION     DATCZV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99833"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..396)
                     /gene="queF"
                     /locus_tag="VIW27_13190"
     CDS             complement(7..396)
                     /gene="queF"
                     /locus_tag="VIW27_13190"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY5740675.1"
                     /translation="MTTQPSTTLETFDNPNPGRDYTIHIDTPEFTCLCPMTGQPDFAT
                     IEIEYVPDRLCLELKALKLYFWSYRDQGAFHEAVTNRILDDLVAAIAPRFMRVNADFN
                     VRGGVYTRVVAEHRADGWIAPEPVDLP"
     gene            563..>2776
                     /gene="smc"
                     /locus_tag="VIW27_13195"
     CDS             563..>2776
                     /gene="smc"
                     /locus_tag="VIW27_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY5740676.1"
                     /translation="MRLSKIKISGFKSFVDPSNIAFPADITGIVGPNGCGKSNVIDAV
                     RWVMGESSARNLRGESMEDVIFSGSSSRQPVGQASVELVFDNSDGRVTGEYAKYNEIS
                     VKRIATRVGKSKYFLNNTRCRRRDIADIFLGTGLGPRSYSIIEQGMVSRIIDARPDEL
                     RTYLEEAAGISKYKERRRETENRIGHTRENLDRLVDLIDEIDNQLSRLDRQAKAAEKF
                     KTLKQEQRQLEAECLLLQKRVFDGEIEAQARQLSAVETAMQERTARLRETEREIEQGR
                     QRLAEASERHNKVQGNYYRLGAEISSKEQAIEHGRNLRQMNRQELAALQQSLAEAERL
                     LAADQARLEQQQQELASLLPAIDTGSAALEERQQAVAAAEGQMHDWQENHDEFRREYH
                     RVHEAAQIENRGIEHIERQVQHTDSRRSRLREELEQLDASAEVREIDALESRVEARSE
                     EYAATMARVQGRADEVRELRETCEQASSRLDEARNRVQSLRGRLSSLQALQQHVLAEA
                     GEEVADWLARNRIDSSRRLAGLLRVRGDVGKAVEVVLAPFLGAYCVTPGAALPDAFVP
                     DRNLAVFDCEAVESPPPARDWPRLADYVDCEIDLAAILDGIYLCGDLQELRQRRPLLG
                     SGESLVTPQGLWAGRNWILQLGEEDQHTGMLAREREIEQIQQQLQQITQQAMALKSRN
                     DADRERLQALERTWDEDQKSLAELQQQLSELRQQLSNRQSQAEQVQQQREQLQQQL"
BASE COUNT          553 a          862 c          949 g          412 t
ORIGIN      
        1 ccgcggctac ggcaggtcga cgggttcagg agcgatccag ccatcggcgc ggtgctccgc
       61 aaccacgcgg gtgtagacgc cgccgcggac gttgaaatcg gcgttgacgc gcatgaagcg
      121 cggcgcgatg gcggccacca ggtcgtcgag aatcctgttg gtcaccgcct cgtgaaacgc
      181 gccctggtcg cggtaggacc agaaatagag tttcagcgcc ttcagctcga ggcacagccg
      241 gtcgggcacg tactcgatct cgatagtggc gaaatcgggc tgtccggtca tcgggcacag
      301 gcaggtgaac tcgggcgtat cgatatggat cgtgtaatcg cgcccgggat tcggattatc
      361 gaaggtttcc agggtggtcg acggctgggt agtcatgatt tggggtcggc ttggttgctg
      421 cgttgcgggg gcaagtctcc gtggcgcgcc attatatcga ggtcgatgca ataggcaatt
      481 aattgcagca gatttactgc cggtcggaat gcctgatgat atactctggc cgcagttttt
      541 cagcctggat tcagccgcac gaatgcgcct cagtaaaatc aagatttcgg gattcaaatc
      601 ctttgtcgat ccgagcaata tcgctttccc ggccgatatt acgggtatcg tcggacccaa
      661 cggttgcggc aagtccaacg tcatcgacgc ggtccgctgg gtgatgggcg aatcgtcggc
      721 gcgcaacctg cgcggcgaat ccatggaaga cgtgatcttc agcggctcct cgagccgcca
      781 gccggtcggc caggcgtcgg tggagctggt gttcgacaat tccgacgggc gggtgaccgg
      841 cgagtacgcc aagtacaacg agatatcggt caagcgcatc gccacgcggg tcggcaaatc
      901 gaagtacttc ctcaacaaca cccgctgccg gcgccgcgat atcgccgaca tcttcctcgg
      961 caccggtctc gggccgcgca gctactcgat catcgagcag ggcatggtgt cgcgcatcat
     1021 cgatgcgcgt cccgatgagc tgcgtaccta cctcgaggaa gccgcgggca tttccaagta
     1081 caaggagcgc cgccgtgaaa ccgagaaccg tatcggtcac acgcgcgaaa atctcgaccg
     1141 cctggtcgac ctgatcgacg agatcgacaa ccagttgtcg cgcctcgacc ggcaggcgaa
     1201 ggcggccgaa aaattcaaga cgctgaaaca ggagcaacgg cagctggaag ccgaatgcct
     1261 gctgttgcag aagcgggttt tcgacggcga gatcgaggcc caggcgcgcc agctgtccgc
     1321 ggtcgaaacc gcgatgcagg agcgcaccgc caggctgcgc gaaaccgagc gcgagatcga
     1381 gcaggggcgg cagcggctcg ccgaggccag tgagcgacac aacaaggtgc agggaaacta
     1441 ctaccggctc ggcgccgaga tctccagcaa ggagcaggcc atcgagcacg gccgcaacct
     1501 gcggcagatg aaccgccagg agctggcggc gctgcagcag tcgctcgccg aggcggaacg
     1561 cctgctggcc gccgaccagg cgaggctcga gcagcagcag caggaacttg caagcctgct
     1621 gccggcgatc gacaccggca gtgcggcgct cgaggagcgg caacaggcgg ttgccgcggc
     1681 cgaggggcag atgcacgatt ggcaggaaaa tcacgacgag ttccgccgcg agtatcaccg
     1741 cgtgcacgag gcggcgcaga tcgaaaaccg cggcatcgag cacatcgagc gccaggtgca
     1801 gcataccgac agccgtcgct cacggctgcg cgaggagctc gagcagctcg acgcgtccgc
     1861 cgaggtcagg gaaatcgacg cgctcgagag ccgggtcgag gcccgcagcg aggaatacgc
     1921 ggcgaccatg gcgcgtgtgc agggccgcgc cgacgaagtc cgcgaattgc gcgaaacctg
     1981 cgagcaggct tcgagccgcc tggatgaggc ccgcaaccga gtgcagtcgc tgcgcggccg
     2041 cctgagctcg ctgcaggcgc tgcagcagca cgtgctcgcc gaggccggcg aggaagtcgc
     2101 cgactggctg gcgcgcaacc gcatcgactc gagtcgccgg ctggccggcc tgctgcgggt
     2161 acgcggcgac gtcggcaagg cggtcgaagt cgtgctggcg ccgtttctcg gcgcttactg
     2221 cgttaccccg ggcgcggcgc tgccggacgc gttcgtgccc gaccgcaacc tcgcggtgtt
     2281 cgattgcgag gcagtggaat cgcccccgcc ggcgcgcgac tggccgcggc tggccgatta
     2341 cgtcgactgc gaaatcgacc tggcggcgat actcgacggc atctacctgt gcggcgacct
     2401 gcaggaattg cgccagcgcc ggccgctgct cggcagcggc gaaagcctgg ttacgccgca
     2461 gggtctgtgg gcgggacgca actggatcct gcagctcggc gaggaagacc agcacaccgg
     2521 catgctcgcg cgcgagcgcg agatcgaaca aatccagcaa cagctgcagc agatcacgca
     2581 gcaggcgatg gcgctgaagt cgcgcaacga cgccgaccgt gaacgcctgc aggcgctgga
     2641 gcgaacctgg gacgaagacc agaaatcgct cgccgaactg cagcagcaac tctccgagct
     2701 gcgccagcaa ctgagcaatc gccagagcca ggccgagcag gtgcagcagc agcgcgagca
     2761 gttgcagcag caattg
//
LOCUS       DATCZV010000583         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_153175, whole genome shotgun sequence.
ACCESSION   DATCZV010000583 DATCZV010000000
VERSION     DATCZV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153175"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VIW27_13200"
     CDS             <1..1253
                     /locus_tag="VIW27_13200"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY5740677.1"
                     /translation="YLSGERADILLPAMPNVHSHAHQRAMAGLSERAGDGEDSFWTWR
                     RVMYHYLQRIQPEQLQHIAAQLYVEMLKAGYGAVGEFQYLHHDLDGRAYDNRAEMSLQ
                     CLEAARRTGIGFTALPVLYRYGGFGGAEPLAAQRRFLNDADGFVDIVEALQQASRGNA
                     DSAVGIAPHSLRAISRELLTEVDAGLQDLAAIHIHIAEQTREVDDCVAWSGRRPVEWL
                     FDNFEVDGGWCLIHATHMTAAETAKTADSGCIAGLCPTTEANLGDGFFNLIDYADHGG
                     SWAIGSDSHISIDPVEELRWLEYGLRLQTGRRNLLASAACQSTGRNLFDAALAGGAQA
                     CGRAIGAIESGRRADVMVLDGGHPRLYGRRRDELIDSWIFSGNQNLVRDVYVGGDKVI
                     ENGHHADEERIARDYRATLDQLAT"
     gene            1278..>2061
                     /locus_tag="VIW27_13205"
     CDS             1278..>2061
                     /locus_tag="VIW27_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY5740678.1"
                     /translation="MAIVLQPGSTGLEDLQRIYREDVEIDIDPRSKPRVDAAAAVVAG
                     AAGRSEPVYGINTGFGKLASTRIPAAQTSQLQRNLILSHCCGVGEPLPPEIVRLVIAL
                     KLLSLGRGASGVRWQILERLRDLLRHDLLPLLPAQGSVGASGDLAPLAHLTAAMIGEG
                     HVFLRGQRMPARDALAAAGLEPLELGPKEGLGLINGTQVSTALALAGLFDAWNLAQTA
                     LISGALTTDALMGSTRPFAAEVHALRGLQGQIDAAQTLRALLE"
BASE COUNT          353 a          706 c          675 g          327 t
ORIGIN      
        1 cctacctgtc gggcgagcgc gccgacatcc tgctgccagc aatgccgaac gtgcactcgc
       61 atgcgcacca gcgcgcaatg gcgggcttaa gcgagcgcgc cggcgacggc gaggacagct
      121 tctggacctg gcgccgagtg atgtaccact acctgcagcg cattcagccc gaacaactgc
      181 agcacatcgc cgcgcaactc tacgtcgaaa tgctcaaggc cggctatggc gcggtcggtg
      241 aattccagta cctgcaccac gacctcgacg gacgagccta cgacaaccgc gccgagatga
      301 gcctgcagtg cctcgaagcc gcgcgtcgta ccggtatcgg ctttaccgcg ctgccggtgc
      361 tgtatcgcta tggcggcttc ggcggcgccg agccgctggc cgcgcaacga cgtttcctga
      421 acgatgccga cggtttcgtc gacatcgtcg aagcgctgca gcaggccagc cgcggcaacg
      481 ccgattccgc ggtcggcatc gcgccgcact cgctgcgcgc gatcagccgc gagttgctga
      541 ccgaggtcga cgccggcctg caggatctcg ccgcgatcca cattcatatc gccgagcaga
      601 cccgcgaagt cgacgactgc gtggcctgga gcggccgccg cccggtcgaa tggctgttcg
      661 acaatttcga agtcgatggc ggctggtgcc tgatccatgc cacccatatg acggctgccg
      721 aaaccgcgaa gaccgccgat agcggttgta tcgccggatt gtgcccgacc accgaggcca
      781 atctcggcga cggtttcttc aacctgatcg attacgccga tcatggcggg agctgggcga
      841 ttggttccga cagccatatc tcgatcgatc cggtcgagga gctgcgctgg ctcgaatacg
      901 gcctgcgcct gcagaccgga cggcgcaacc tgctggcctc cgccgcctgt caaagcaccg
      961 ggcgcaacct gttcgacgcc gcgctggccg gcggagcgca ggcctgcggg cgcgccatcg
     1021 gcgccatcga aagcggtagg cgtgccgatg tcatggtgct cgatggcggg catccgcggc
     1081 tttacggccg ccgccgggac gaactcatcg acagctggat attttccggc aatcagaacc
     1141 tggtgcgcga tgtctacgtc ggcggcgaca aggtgatcga aaacgggcac catgccgacg
     1201 aggaacgaat cgcgcgcgac taccgcgcga ccctcgatca actggcaacc tagcctgagc
     1261 gggagaaaag acagaccatg gccatcgtat tgcagcccgg ctcgaccggc ctcgaggacc
     1321 tgcaacgcat ctaccgcgag gatgtcgaga tcgacatcga cccgcgcagc aaaccgcgag
     1381 tcgacgccgc cgccgcggta gtcgccggcg cagccggcag aagcgagccg gtctacggta
     1441 tcaataccgg tttcggcaag ttggcctcga cgcgcattcc ggcggcacag acctcacagt
     1501 tgcagcgcaa cctgatcctg tcgcactgct gtggcgtcgg cgagccgctg ccgccggaga
     1561 tcgtgcgcct ggtgatcgcc ttgaaactgc tgtcgctggg gcgcggcgcg tccggcgtcc
     1621 gctggcagat cctcgagcgc ctgcgcgact tgttgcgcca cgacctgctg ccgctgctgc
     1681 cggcccaggg ttcggtgggc gcgtccggcg acctggcgcc gctcgcccac ctgacagcgg
     1741 cgatgatcgg cgaaggtcat gtctttcttc gcggtcagcg aatgccggcg cgcgacgcgc
     1801 tcgccgccgc cgggctcgaa ccgctggaac tcggccccaa ggaaggtctc ggcctgatca
     1861 atggtaccca ggtatcgacc gcgctggcgc tggccggcct gttcgacgcc tggaacctgg
     1921 cacagactgc gctgatctcc ggcgcgctga ccaccgacgc gctgatgggc tcgacgcgtc
     1981 cgttcgccgc cgaggtgcac gcgctgcgcg gactgcaggg gcagatcgat gctgcgcaaa
     2041 ccctgagagc gctgctcgag g
//
LOCUS       DATCZV010000584         3670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_630292, whole genome shotgun sequence.
ACCESSION   DATCZV010000584 DATCZV010000000
VERSION     DATCZV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630292"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VIW27_13210"
     CDS             complement(<1..721)
                     /locus_tag="VIW27_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5740679.1"
                     /translation="MARRHYQLPSLTGLNTFDACARHGSFTAAATELGVTLGAVSRQV
                     KALEADLGCALFLRHHRGVELTAEGGDLFEVLSSGFRQIGRKCEVLRNRQQSSDVTIG
                     ATTALASLWLMPRLGGFWRSHADVNLSHAISDNPDDSGFVNADIRIRYGAGNWAGEQS
                     EHLFDDRIYPVCGRELAERHEFGHPADLLQFPLLQLDSVDPAWTGWEAWLAHWQCDLS
                     KAKIRRFNNYVVALHAAAENQG"
     gene            853..2028
                     /locus_tag="VIW27_13215"
     CDS             853..2028
                     /locus_tag="VIW27_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5740680.1"
                     /translation="MSQHTVSEADLDAILQPIDRATGLPNPAYTSDEFFVREREQVFA
                     NSWAGLWFASDLPKKGYVKPVEFMGLPLIILRNKQDEIRVFHNVCSHRGMLLVHSEME
                     VQGALRCKYHSWSYNLDGELKGTPHLGGIGQQRVDGFDHCAHGLKPVRSTVWMDVIFI
                     NLSGDALDFDTYIKPLTEKWQPFLGSTGMERMRSAVNGKGLEIEVKSNWKLAVENYCE
                     SYHLPWIHPSLNTYSRLEDHYHIMYGDDFGGQGSYAYRLCDEVGTALPRFPEWPEDKL
                     EQAEYVAFYPNVLLGIQADHTFAMIVEPTAAKRAKEHLRLYYVGDEALGDAHAASRNA
                     TLESWRVVFGEDIDAVEGMQRGRLSPGYRGGVFSPVMDNPTHHFHQWVARKLRAASA"
     gene            complement(2029..>3670)
                     /locus_tag="VIW27_13220"
     CDS             complement(2029..>3670)
                     /locus_tag="VIW27_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY5740681.1"
                     /translation="FRRQLPFEHGAVQTIAVLFVDQLAGGHGALRIAVPEAQRAVATG
                     KLVDEQHGDGLYRAVFERQLAAEAPSVFAGPYRVGERMQDGIRLRTYFHAELEGFADV
                     YLDTAGSYIRRYADAIGDYPYADFHIISAPLPVGLGFPNLTYVGRKIVPLPFMRGRSL
                     AHEVLHNWWGNGVAVDYARGNWAEGLTTYQADYALARDQGADAARAMRVKWLRDYAAL
                     PKERDLPVRMFRSKQHDASQVIGYNKVAFIFHMLRVEIGQQAFDEGLRIFWREKRFRT
                     AGWDDLRAAFERSSGRTLDWFFSQWLERAGAPRPALGAHRVEQVDDGYLTSVELLQPV
                     AGYRFQLPVLLVTEDGLERHQIAVSESLTRVQWTTDARPLSIQFDPDSDVFRKLQSGE
                     APPILRDITLDPEARTVILSGDDEFGEIAKQLAGRLLESAPRVEGLERAQQSKRPLLV
                     ITRGDRLPDLLERLQIGLPPELAGTNHSAAAWTARRPAGNPVLVISAENSGELQSLLR
                     PLPHYGGQSYVLFAAGRALQRGIWPITRGALYRELDDR"
BASE COUNT          737 a         1159 c         1122 g          652 t
ORIGIN      
        1 taccctggtt ttccgcggcg gcatgcagcg cgacgacata attattgaag cgcctgatct
       61 tcgctttcga caggtcgcat tgccagtgcg cgagccaggc ttcccagccg gtccaggccg
      121 ggtcgacgct gtcgagttgc agcagcggga attgcagtaa atcggccgga tgaccgaatt
      181 cgtgacgttc ggctaactcg cgcccgcaga ccgggtagat gcggtcgtca aacagatgct
      241 cggactgctc cccggcccag ttgccggcac cgtaacggat gcggatgtcg gcgttgacga
      301 agccgctgtc gtcgggatta tcggaaatcg cgtggctcag attgacatct gcgtgactgc
      361 gccagaagcc gccgagccgc ggcatcagcc acagcgaggc gagcgcagtg gtcgcaccga
      421 tggtgacgtc cgaactctgc tggcggtttc ggagcacctc gcacttgcgg ccgatctggc
      481 gaaatccgct gcttagcacc tcgaacagat cgccgccttc cgcggtcagt tcgacgccgc
      541 gatgatgacg caggaacagc gcacagccga gatcggcttc cagcgccttg acctgacggc
      601 tcaccgcacc gagggtgaca ccgagttcgg tagccgcggc ggtaaagctg ccgtgacgcg
      661 cacaggcatc gaaagtattc agaccggtga ggctgggtaa ctggtagtga cggcgagcca
      721 tattctgctg aagtttacct taagtaaagc catatcgaag ttttttaata ttgctaaaga
      781 cggcaattca tagagaatta atcggattac cggcatcttc tccccaccgg gcttgatttg
      841 agggcaacac ctatgagcca gcataccgtt tccgaagccg atctggatgc aatcctgcaa
      901 ccgatcgaca gggcgaccgg tttacccaac ccggcttaca ccagtgatga attcttcgtg
      961 cgtgagcgcg agcaagtgtt cgccaatagc tgggccgggc tgtggtttgc cagtgacctg
     1021 cctaaaaagg gttacgtcaa gccggtcgaa ttcatgggcc tgccgctcat catcctgcgc
     1081 aacaagcagg acgaaatccg cgtgtttcac aacgtttgta gccaccgcgg catgctgctg
     1141 gtgcacagcg aaatggaagt ccagggggca ctgcgctgca agtaccattc ctggtcctac
     1201 aacctggacg gcgaactcaa gggcacgccg catctcggcg gtatcggtca acagagggtc
     1261 gacggcttcg atcactgcgc ccacggcctg aagccggtgc gctcgaccgt ctggatggat
     1321 gtcatcttca tcaacctgtc cggcgacgca ctcgacttcg acacctatat caaaccgctg
     1381 acggaaaaat ggcaaccctt tctcggcagt accggcatgg agcgcatgcg ctcggcggtc
     1441 aacggcaagg gcctcgagat cgaagtcaaa tcgaactgga aactcgcggt cgaaaactac
     1501 tgcgaaagct accacctgcc ctggatacat ccgagcctga atacctattc gcgcctcgag
     1561 gatcactacc acatcatgta cggcgacgat ttcggcggcc agggcagcta tgcgtatcgt
     1621 ctctgcgatg aagtcggcac ggcgttaccg cgcttccccg aatggcccga agacaaactc
     1681 gaacaggccg agtacgtcgc tttctacccc aacgttttgc tcggcatcca ggccgaccat
     1741 accttcgcaa tgatcgtcga accgacagcc gccaaacgcg caaaggaaca tctgcgcctg
     1801 tactacgtcg gcgacgaagc gctcggcgac gctcacgcgg cgagccgcaa cgcgaccctg
     1861 gaatcctggc gggtggtgtt cggcgaagat atcgacgcgg tcgagggcat gcagcgcggc
     1921 cggctatcgc cgggttaccg gggcggggtg ttttcgccgg tgatggacaa tcccacccac
     1981 cactttcacc aatgggtggc gcgcaagctg cgcgccgcca gcgcctgatc agcggtcgtc
     2041 gagctcgcgg tagagcgcgc cgcgcgtgat cggccagatg ccacgctgga gcgcgcgacc
     2101 ggcggcgaac agcacgtaac tctggccgcc gtaatgcggc aaaggccgca gcagggattg
     2161 cagttcgccg ctgttctcgg cactgatcac cagcaccggg ttgcccgcgg gccgtcgcgc
     2221 ggtccacgcc gccgcggaat gattcgtacc cgccagctcc ggcggcaaac cgatttgcag
     2281 tcgctcgagc aaatccggca ggcgatcgcc gcgggtgatc accagtagcg gtcgcttcga
     2341 ttgctgcgcg cgctccagtc cctcgacgcg cggggcggat tcgagcaggc ggccggcgag
     2401 ctgctttgcg atctcgccaa attcatcatc gccagacaag ataaccgtgc gcgcttccgg
     2461 gtccagcgtg atgtcacgca aaatgggtgg cgcctcaccc gactgtaatt ttcgaaacac
     2521 atcgctgtcg ggatcgaact gaatcgacag cgggcgcgcg tcggtggtcc attgcacccg
     2581 ggtcagcgat tcgctgaccg caatctgatg gcgctcgagc ccgtcctcgg tgaccagcaa
     2641 aaccggtaac tggaatcgat atccggccac cggctgcaat agttcgacac tggtaaggta
     2701 gccgtcgtca acctgctcga cccggtgcgc gccaagcgcc ggacgcggtg cgccggcacg
     2761 ctccagccac tggctgaaga accagtcgag cgtgcggccg gaactgcgtt cgaaggccgc
     2821 gcgcaagtcg tcccagccgg cggtgcgaaa acgcttttcg cgccagaata tcctcagccc
     2881 ctcgtcgaaa gcctgctggc cgatctcgac gcgcagcatg tgaaagatga aggccacctt
     2941 gttataaccg atgacctgtg acgcatcgtg ctgcttcgac ctgaacatgc gcaccggcag
     3001 atcgcgctcc ttcggcagtg ccgcgtaatc gcgcagccac ttgacgcgca tcgcgcgcgc
     3061 agcgtcggcg ccctggtcgc gtgccagcgc ataatcagcc tgataggtcg tcaggccctc
     3121 ggcccagtta cccctggcgt aatcgaccgc gaccccgttg ccccaccagt tgtgcagcac
     3181 ttcgtgggcc agcgagcggc cgcgcatgaa cggcaacggc acgatcttgc gcccgacata
     3241 ggtcaggttg gggaacccga gcccgaccgg taaaggcgcg gatatgatat ggaaatcggc
     3301 atagggataa tcgccgatag cgtcggcgta acgccgaata taggaaccgg cggtatccag
     3361 gtagacgtcg gcgaaaccct cgagttcggc atgaaagtaa gttcgcaagc gaatcccgtc
     3421 ctgcattcgc tcgcctaccc ggtagggacc ggcgaacacc gagggcgctt ccgccgccag
     3481 ttgccgttcg aacacggcgc ggtacagacc atcgccgtgc tgttcgtcga ccagcttgcc
     3541 ggtggccacg gcgcgctgcg cctcgggtac cgcgatgcgc agcgcgccgt ggccaccggc
     3601 aagctggtcg acgaacagca cggcgatggt ctgtaccgcg ccgtgttcga acggcaactg
     3661 gcggcggaag
//
LOCUS       DATCZV010000585         3759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_630324, whole genome shotgun sequence.
ACCESSION   DATCZV010000585 DATCZV010000000
VERSION     DATCZV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630324"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..713)
                     /locus_tag="VIW27_13225"
     CDS             complement(60..713)
                     /locus_tag="VIW27_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY5740682.1"
                     /translation="MPESHYLFLILSWLVYFGLHSLLASIAAKQAVASRWPRAMPAYR
                     LGFNVLAVLLLALPLWLTLSDSGPWLWRWTGAAFAVSAALSAAAVAGFLWSLKFYDGR
                     EFLGLRQLSEGNASVDDQEQFQLSPLHRRVRHPWYFFALVIIWTRDMNVSMLITAILM
                     TLYFVIGSRLEERKLLRYHGEVYRRYRERVPGLIPLPWKYLRAAEARDLTGENHRDS"
     gene            1155..3515
                     /locus_tag="VIW27_13230"
     CDS             1155..3515
                     /locus_tag="VIW27_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEY5740683.1"
                     /translation="MALMYPDTHKARVIFSSRAEEVFYDACRAQLGDRWTVYFSRTLS
                     TIERDKGLHDNEIDFVLYHPELGVLVLEVKGGRIRHDADSGEFFSINRYDESFRIKDP
                     FQQALVWKSRFWRLLRNRNLKVPVSHAVVFPSVHENEILESASITPEIVVGRQKLKSL
                     ASALRNMVHKVQPRKYLKFDDVADDLHTILWGQAFTSKLYLKDYLASHDLRVKDIEVI
                     QETLVQPIAASARLAVEGEAGTGKTLIALLLARYFRGAGKRVLILSSNRLLNAYLRNE
                     VGANVDVKTYVEIGKDFGVHLLQPPADYAGKREDWIEYEAPEQLLRALQNSELRYDVL
                     ICDEAQDVQTFWWEALETLLDEADPRFYIFFDRSQGVFVQGGGGRGFEPESVLPIAQP
                     YFPLVYNYRTTREIAGFSRAFRTGDRVMQSHCGRLGYIPEIVLYDDAQDCRGKLGRLF
                     RKLFREEGVATDDVTLLSARNPNTPESVLRPSDVIARHGLRYLGDTEQFGPRATANNQ
                     VGLSTIAGFKGLETPIAVLLNISEYNLPLDNPIMASLIYVACTRAKHMLYVMVQRDDP
                     KREMLEQALKQIRTTGAMVLEGSDANFEFVGTVSHYNPERIGWLEVSDPAIEKNSIMF
                     FPHDARQAGIDDLQIGDRVRFRLQVEGQVTIAAELKRVDMEALAAAAEAEAEESGDAQ
                     AEDEIDAAPQNDNANDDEPPANGTAASVAADFDPGPRYKAAAAAEPASPARSSPAKGN
                     DSAPQTDASPASASVTPLKPKSAARKRETLGVKPGRREPRKKRAPD"
     gene            complement(3512..>3759)
                     /locus_tag="VIW27_13235"
     CDS             complement(3512..>3759)
                     /locus_tag="VIW27_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740684.1"
                     /translation="DLNLMERVYSHVYGVQLLVSGEPRLWFRPWQLRKPGSDAKFDAA
                     TACRKSPAQRETNVQRVRGPALAIAPAVRCRKPETAL"
BASE COUNT          773 a         1232 c         1141 g          613 t
ORIGIN      
        1 gcccgggatg acgatgctgc tctccgcctg cccctaccct cgcggctggc gcaaagcagt
       61 caactgtcgc gatgattttc gccggtcagg tcccgcgcct cggcggcccg caaatatttc
      121 cacggcagcg gtatcaaccc tggtacccgc tcccggtagc ggcgataaac ctcaccgtga
      181 tagcgcaaca gtttgcgctc ctcgagtcgc gagccgatga cgaaatacag cgtcatcaaa
      241 atcgccgtga ttagcatcga cacattcatg tcgcgcgtcc agatgatgac cagcgcgaaa
      301 aaataccagg gatgacgcac ccggcgatgc agcggcgaaa gctggaactg ttcctggtcg
      361 tcgacactgg cgttaccttc gctgagttgc cgcagcccga gaaactcgcg gccgtcgtag
      421 aacttcagcg accacaaaaa ccccgccacc gccgccgccg agagcgcagc cgagacagcg
      481 aaagcggcac cggtccagcg ccacaaccag ggccccgaat cgctcagggt caaccacagc
      541 ggcaacgcca gcagcaaaac cgcgagcaca ttgaatccca gccggtaagc cggcatcgct
      601 cggggccagc gcgaggcgac ggcctgcttc gccgcgatcg atgcgagtag cgagtgcagg
      661 ccgaagtaga cgagccagct cagtatcaga aacaggtaat gggattcggg cattcgtcat
      721 tcatcgcatt cgcggaactc gctagactag cccgcgagtt cataaggctc aagcaccgat
      781 caccctgaac gattgccgcg ataccattca tcgatagcgg gtcaggtaag cgctcaacag
      841 accgacttcc gggactgcga tccccggcct gaccgaattt accgaacctt gcggccgccc
      901 cagggccggt atcgcgctcc cgatgcgccg gttcgggaag cgggctggtc gagagaagag
      961 gcccgcactg gcatggtaat ggagagggcc gtggctacct cagggttatc gggtttttga
     1021 tcttttaact cgagaactcg aaatcttggg cctgccaccc ggccgtcttc ttcggtgggc
     1081 gctggcgcgg cgggtaaact ttcctatact catgggctaa cggaccgaca cgccgtcctc
     1141 gccggaggga tcccatggcc ctgatgtacc ccgacaccca caaggcgcgt gtcatcttct
     1201 ccagccgcgc cgaggaagtg ttttacgatg cctgccgcgc acagctcggc gaccgctgga
     1261 cggtttactt ctctcgcacc ctgtcaacca tcgagcgcga caagggcctg cacgacaacg
     1321 agatcgactt cgtgctctat cacccggagc tcggcgtgct ggtgctcgag gtcaagggcg
     1381 ggcgtattcg ccatgatgcc gacagcggcg agtttttttc catcaaccgt tacgacgaga
     1441 gcttccgcat caaggacccc ttccagcagg cgctggtgtg gaagagccgc ttctggcggc
     1501 tgctgcgcaa ccgcaacctg aaggtgccgg tgagccacgc ggtggtgttc ccgtcggtac
     1561 acgaaaacga gattctcgaa tccgcgtcga tcacgccgga gatcgtcgtc ggccgccaga
     1621 aactgaaatc actcgccagc gcgctcagga acatggtgca caaggtgcag cccaggaaat
     1681 atctcaagtt cgacgacgtg gccgatgacc tgcacaccat cctctggggc caggccttca
     1741 ccagcaagct gtatctgaag gactatctcg cgagccacga cctgcgggtc aaggacatcg
     1801 aggtcatcca ggaaaccctg gtacagccaa tcgcggcctc ggcgcggctc gctgtcgagg
     1861 gcgaggcggg taccggcaag acgctgatcg cgctgctgct ggcacggtat tttcgcggcg
     1921 ccggcaagcg tgtcctgatc ctgtcgtcga accggctgct aaacgcctac ctgaggaatg
     1981 aggtcggcgc caacgtcgac gtgaaaacct acgtcgagat cggcaaggac ttcggcgtgc
     2041 acctgctgca gccacccgcc gattatgccg gcaagcgcga ggactggatc gagtacgaag
     2101 caccggaaca gctgctgaga gcgctgcaga actcggagtt gcgctacgac gtgctgattt
     2161 gcgacgaggc gcaggatgtg cagactttct ggtgggaggc gttggagacc ctgctcgacg
     2221 aggccgaccc gcgcttctac atcttcttcg atcgcagcca gggggtgttc gtgcagggcg
     2281 gcggcgggcg cggcttcgag cccgagagcg tactgccgat cgcgcagccc tacttcccgc
     2341 tggtgtacaa ctaccgcacc acgcgcgaaa tcgccggttt ctcgcgcgcc tttcgtaccg
     2401 gcgaccgagt catgcaaagc cattgcgggc ggctcggtta catccccgag atagtgctct
     2461 acgacgatgc gcaggattgc cgcggcaagc tcgggcggct gtttcgcaag ctgttccgcg
     2521 aggagggcgt ggccaccgac gacgttaccc tgctgtcggc gcgcaacccg aatacgcccg
     2581 agtcggtgct caggccgagc gacgtcatcg cgcgccacgg cctgcgctat ctcggcgaca
     2641 ccgagcaatt cggcccgcgg gccaccgcca acaaccaggt gggtctgtcc accatcgccg
     2701 gcttcaaggg tctggaaacg ccgatcgcgg tgctgctcaa catcagcgag tacaatttgc
     2761 cgctcgacaa cccgatcatg gcgagcctga tctacgtcgc ctgcacccgc gccaaacaca
     2821 tgctctacgt aatggtgcaa agggacgacc ccaagcgcga gatgctggaa caggcgctga
     2881 aacagattcg caccaccggc gccatggtgc tcgagggctc ggacgccaac ttcgaattcg
     2941 tcggcacggt cagccattac aacccggagc ggatcggctg gctggaagtg agcgacccgg
     3001 cgatcgaaaa aaattcgatc atgttctttc cgcacgacgc gcgccaggcc ggcatcgacg
     3061 atctgcaaat cggcgaccgc gtgcgcttcc gcctgcaggt ggaaggccag gtcaccatcg
     3121 ctgccgaact gaagcgcgtg gacatggaag cccttgccgc cgcggcggag gcggaggcgg
     3181 aagagtcagg cgacgcgcaa gccgaggacg agatcgatgc ggcaccgcag aatgacaacg
     3241 cgaatgacga cgaaccgccg gcgaatggca cagccgcatc cgtggcagcg gacttcgacc
     3301 ccgggccacg atacaaggca gcagcggccg cagaacccgc gtcgccagcc cgatcgtccc
     3361 cggcaaaggg caacgacagc gcaccgcaaa ccgacgcgtc acccgccagt gcgtcggtca
     3421 cgcccctgaa acccaaatcc gcagcgcgca aacgcgagac cctgggcgtc aagcccggca
     3481 ggcgcgaacc gcgcaaaaaa cgcgcccctg attagagagc ggtttccggt ttccgacacc
     3541 gtactgccgg cgcgatagcc aaagccgggc cccgtacccg ctgcacgttt gtttcccgct
     3601 gcgcaggcga ctttcgacag gccgtcgcgg cgtcgaactt tgcgtcgctt cccggcttcc
     3661 tgagctgcca cgggcgaaac cataaacgcg gttcgccact caccagtagc tgcacaccgt
     3721 agacatgact gtagacccgc tccatcaggt tgaggtcca
//
LOCUS       DATCZV010000586         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_470572, whole genome shotgun sequence.
ACCESSION   DATCZV010000586 DATCZV010000000
VERSION     DATCZV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470572"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VIW27_13240"
     CDS             complement(<1..138)
                     /locus_tag="VIW27_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase"
                     /protein_id="HEY5740685.1"
                     /translation="MSASLEKGINALKEQVDSTVATFFTSCVHCGICAEACLFYTETG
                     DA"
     gene            complement(135..794)
                     /locus_tag="VIW27_13245"
     CDS             complement(135..794)
                     /locus_tag="VIW27_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740686.1"
                     /translation="MTEIEFLSWVRGPGFQIATIVFVGGIVVRLLETLLLGRAPNLAE
                     PKGPAISSGFKAIFMRSFSTTGKFRRVMFTLVAGYIFHIGFLVTLFFYAPHILLFKDV
                     TGLSWPSLPTPIVDALTVVSIIALLAVLVHRLTSKVLRHLSKFEDWLVWFLTIAPLAT
                     GYIAFHRIGGTAPTLLAIHILSVELLMIVFPFTKLMHAFTLFIGRWYNGAMSGYRGVQ
                     P"
     gene            complement(856..1956)
                     /locus_tag="VIW27_13250"
     CDS             complement(856..1956)
                     /locus_tag="VIW27_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEY5740687.1"
                     /translation="MSDRKARPPVTLGESLRAQGVSRRSFLKFCAATTSMMALSPALI
                     PRVAHALENAKRPSVIWLSFQECTGCTESLTRAHTSTVESLIFDAISLDYHHTLQAAS
                     GHAAEAAREAAMADNHGNYLLVVDGSIPLGNPGYSTIAGISNLDMLESTAAGAAAVIA
                     VGSCASFGGIPHADPNPTGAVSVADIVKDKPIINVPGCPPIPVVITGVLAHFLTFGSI
                     PELDHLGRPLVFFGKSIHDRCYRRPFYDRGLFAETFDDEGAKKGWCLYKLGCKGPTTY
                     NACATTKWNNGTSFPIEAGHPCIGCSEPDFWDGGGFYKAISMPTENISKTAVYAAAGA
                     AAAGVAIGAANRARKKALKAAHSKVGVDDLEK"
     gene            complement(1953..>2311)
                     /locus_tag="VIW27_13255"
     CDS             complement(1953..>2311)
                     /locus_tag="VIW27_13255"
                     /inference="COORDINATES: protein motif:HMM:NF016682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression/formation C-terminal
                     domain-containing protein"
                     /protein_id="HEY5740688.1"
                     /translation="HALQELADTGSTGIIDLRSIPLAPGEEDKILATLGQGEVHAHVD
                     ALGPSEVIETRYAGVWLVTHYNEDEEILGRFIEITPLPDILRSQAEDIRHACGQLEDY
                     LERTRRSPATSKETQS"
BASE COUNT          422 a          690 c          774 g          425 t
ORIGIN      
        1 cgcgtcgccg gtctcggtgt agaacaggca ggcctcggca cagatgccgc agtgcacgca
       61 gctggtgaaa aaggtcgcga ccgtcgaatc gacctgctcc ttgagggcat tgatgccttt
      121 ttccagtgac gcgctcatgg ctgcaccccg cggtaacccg acatcgcgcc gttgtaccag
      181 cgcccgatga acaaggtgaa ggcatgcatc agcttggtga acggaaacac gatcatcagc
      241 agttcgacgc tcaggatgtg gatcgcgagc agtgtcggcg cggtgccgcc gatacgatgg
      301 aaggcgatat agccggtcgc cagcggcgcg atggtcagga accagaccag ccagtcctcg
      361 aatttgctca ggtggcgcag caccttgctg gtcaggcgat gcaccagcac cgcgagcagc
      421 gcgatgatgc tgaccaccgt cagcgcatcg acgatcggtg tcggcagcga cggccagctg
      481 aggccggtga catccttgaa caggaggata tgcggcgcgt aaaagaacag cgtgaccaga
      541 aaaccgatat ggaatatgta gccggcgacg agggtaaaca tcacgcgcct gaactttccg
      601 gtggtggaaa acgatcgcat gaagatagcc ttgaagccgc tgctgatcgc cggtcccttg
      661 ggctcggcga ggttcggcgc ccggccgagc agcagggttt ccagcaggcg cactacgatg
      721 ccgccgacaa aaacaatggt cgcgatctgg aaaccagggc cgcgtaccca gctcaaaaac
      781 tcgatttcag tcatgcgatg tgtctcctct tactgtgcct gcccgccagg gttgcaggga
      841 tgccggttgt cacgctcatt tctccaggtc gtcgacaccg accttgctgt gcgccgcctt
      901 gagcgccttt ttgcgggcgc ggttggcggc gccgatggcg acgccggcag cggccgcgcc
      961 ggcggcggcg taaaccgcgg tcttggaaat gttttcggtc ggcatggaaa tcgccttgta
     1021 gaaaccgccg ccgtcccaga aatcgggttc ggaacagccg atgcacggat gtcccgcctc
     1081 gatcgggaag ctggtgccgt tgttccactt ggtggtggcg caggcattgt aggtggtcgg
     1141 tcccttgcag ccgagcttgt acaggcacca gcccttcttg gcgccctcgt cgtcgaaagt
     1201 ctcggcgaac aggccccggt cataaaacgg ccgccggtag cagcggtcgt gaatgctctt
     1261 gccgaaaaaa accagcggac ggccgaggtg gtcgagttcc gggatcgagc cgaaggtcag
     1321 gaagtgggcc agcacgccgg tgatgacgac cggtatcggc gggcagccgg ggacgttgat
     1381 gatcggcttg tccttgacga tgtcggccac cgaaaccgcg ccggtcgggt tcggatcggc
     1441 atgcggaatg ccgccgaaag acgcgcaact gccgaccgcg atgaccgcgg cggcgccggc
     1501 cgcggtcgac tccagcatgt cgagattgct gatgccggcg atggtcgagt agccgggatt
     1561 gccgagcgga atcgaaccgt cgaccaccag caggtaatta ccgtggttgt cggccatcgc
     1621 ggcttcgcgc gcggcttccg cggcatgacc ggaggcggct tgcagcgtgt gatggtaatc
     1681 gagcgagatg gcgtcgaata tcaggctctc gacggtcgag gtgtgcgccc gtgtcagtga
     1741 ctcggtgcag ccggtgcact cctggaacga cagccagatc accgacggtc gtttcgcgtt
     1801 ttcgagcgcg tgcgcgaccc gcggaatcag cgccggcgac agcgccatca tcgacgtcgt
     1861 ggcggcgcag aacttcagga agctgcgcct cgatacgccc tgcgcgcgca gggattcgcc
     1921 gagggttacg ggtggcctgg ctttgcgatc gctcatgatt gagtctcctt cgaggttgcc
     1981 ggactgcgtc gggtccgttc caggtaatct tcgagctggc cgcaggcgtg gcggatatcc
     2041 tcggcctgcg agcgcagaat gtcgggcagc ggggtaattt cgatgaaacg gccgaggatc
     2101 tcctcgtctt cgttgtaatg agtcaccagc cagactcccg catagcgggt ttcgatgacc
     2161 tcgctcggcc ccagcgcatc gacgtgcgcg tgtacttcgc cctgcccgag cgtggcgagg
     2221 attttgtctt cctcgcccgg cgccagcggg atactgcgca agtcgatgat gccggtgctg
     2281 ccggtatccg cgagttcctg cagcgcgtgc c
//
LOCUS       DATCZV010000587         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_578836, whole genome shotgun sequence.
ACCESSION   DATCZV010000587 DATCZV010000000
VERSION     DATCZV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578836"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VIW27_13260"
     CDS             complement(<1..560)
                     /locus_tag="VIW27_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740689.1"
                     /translation="MMRIAVFFLLLLLAATWPWPLSAQDDPETRAREVELEELRSRIG
                     RLKQSIGDSAAQRDKLSRALKKDEQAIEAKRKKLAAIEAERKRSEARQARVAAEKSVR
                     EKALRAEMEQLASQARSAYMNGRQERTRLLLNQQDPATLGRMLRYYEYLSKYRGEQIQ
                     AVSAKLAELNRLGAEVEAEEAQLAALA"
     gene            724..1188
                     /locus_tag="VIW27_13265"
     CDS             724..1188
                     /locus_tag="VIW27_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5740690.1"
                     /translation="MDRFFEFANNHTLLVAALFGSFFLAIFSELRRKAQGLINLDAQS
                     SVGLINQGAAIIDLRSQEAFSRGHIVNARNVPMADLEARRELIEKLKGNPILFVCEHG
                     NSSVRAAQSLRKEGFDAVFSLKSGLAAWSQAGLPLVTASKKGKKSQKGQKGQ"
     gene            1212..1493
                     /gene="grxC"
                     /locus_tag="VIW27_13270"
     CDS             1212..1493
                     /gene="grxC"
                     /locus_tag="VIW27_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011748866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY5740691.1"
                     /translation="MTMPARILIYTSPFCMHCHAALALLERKGVDVEQINVAMVPGAR
                     AEMVERSGRRSVPQIFIDGEHIGGNDELRALDVSGELDRRLGLAGARAS"
     gene            1536..2012
                     /gene="secB"
                     /locus_tag="VIW27_13275"
     CDS             1536..2012
                     /gene="secB"
                     /locus_tag="VIW27_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY5740692.1"
                     /translation="MTKKAKSPERQVGIQKIYVKDFSFESPQSPDVFAGGKEWKPHSS
                     LNLRSAHKQIKEKENLFEIVLTITVETKQDDTALFLVEVQQAGVFQITGYEKDEFAHV
                     VGSFCPSVLFPYAREAIASVVSRGGFPEFILQPINFDALYAQSLQQQKDAAGEEAH"
     gene            2018..>2954
                     /locus_tag="VIW27_13280"
     CDS             2018..>2954
                     /locus_tag="VIW27_13280"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5740693.1"
                     /translation="MSDRPAIAVIGAGSWGTALALQLARNDRPVRLWGRDKEQVAAMQ
                     RDRVNARYLPGVPFPDTLRVFGDIKECLDGVRDILIVVPSHALRETLNRIRPLLASDA
                     RIAWATKGFELHSGKLPHQVAAEVLGDRHAMAVLSGPTFATEVGAGLPTALTVAGNDE
                     GFARDLAESLSGENFRAYTSSDVVGVEVGGAVKNVLAIGAGITDGLGFGANTRIALIS
                     RGLVEMTRLGVALGAQADTFRGLAGMGDLVLTCTDDQSRNRRMGLQLASGKTIGEAQE
                     AIQQVVEGVMAAEAVNEVAEGLGVEMPICKEIYRIL"
BASE COUNT          549 a          892 c          946 g          567 t
ORIGIN      
        1 gccagcgccg cgagctgcgc ctcttccgcc tcgacctctg cgccgagccg gttgagctca
       61 gccagcttgg cgctcaccgc ctgtatctgc tcgccgcgat atttgctcag gtactcgtag
      121 tagcggagca ttcgccccag cgtcgccgga tcctgctggt ttagcagcag ccgggttcgc
      181 tcctggcggc cattcatgta agccgatcgc gcctggcttg cgagttgctc catttccgcg
      241 cgcagcgctt tttcgcgaac tgacttttct gcggcaaccc gggcctgccg tgcctcactg
      301 cgtttgcgct ctgcctcgat ggcggcaagt ttcttgcgct ttgcctcgat ggcctgctcg
      361 tcttttttca gcgcccgcga gagcttgtct cgctgtgccg cgctgtcgcc gatactctgt
      421 ttcaggcggc cgatgcgtga gcggagctct tccagctcga cttcgcgagc ccgggtctcc
      481 gggtcgtcct gtgcagacag cggccacggc caggtggcgg ccagcagcag gagcaggaag
      541 aaaacggcga tacgcatcat agggcaggca gtttagagcc tttacccgcg gccgccaaag
      601 gggatcgaca aaatcgctcc ccggcgcccg ttttcagtcg gcggcaggcg gccggcgctg
      661 gtatacttcg ccccctgaat ggtgactgcg ggcgcctgcg cggctcgaac tggtaaggat
      721 tgtatggaca ggtttttcga attcgccaat aaccacacgc tgctggttgc cgcgctgttc
      781 ggcagcttct tcctggcgat tttcagcgag ctcaggcgca aggcgcaggg cctgatcaac
      841 ctcgatgcgc aaagctcggt cgggctgatc aaccagggcg cggccatcat cgatttgcgc
      901 agccaggaag ctttctcgcg cggccacatc gtcaacgccc gcaacgtacc gatggctgac
      961 ctcgaggctc gaagagagct tattgaaaaa ctgaagggca atccgatact gtttgtctgc
     1021 gaacacggca acagctccgt gcgcgcggca cagtctttgc gcaaagaggg ctttgatgcc
     1081 gtctttagcc tgaagagcgg cctcgcggcg tggtcgcagg ccggtctgcc gctcgtcact
     1141 gccagcaaga aaggcaagaa gagccagaag ggccaaaagg gccagtgagc tcggcgcccg
     1201 agcgcggata catgacgatg cctgccagga ttttgattta tacgtcgccg ttttgcatgc
     1261 actgtcatgc ggcgctcgcg ctgctcgagc gcaagggagt cgatgttgaa cagatcaacg
     1321 tggcaatggt tcccggtgcg cgcgccgaga tggtcgaaag gagcggtcgc cgctccgtcc
     1381 cgcagatttt tattgatgga gaacatatcg gcggcaacga tgagctcagg gcgctggatg
     1441 tttccggcga actcgacagg cggctggggc tcgcaggtgc ccgcgcctcg taacgaaaat
     1501 taactcgtaa tgaaactcga caggtagaaa agcacatgac aaaaaaagcg aaatccccgg
     1561 aacgccaggt tggcattcag aaaatttacg tcaaggattt ctcctttgaa tcgccacaaa
     1621 gcccggatgt ttttgccggc ggcaaggagt ggaagccgca cagcagcctt aaccttcgca
     1681 gcgcccacaa acagatcaag gagaaggaaa atctcttcga aatcgttctg acgatcaccg
     1741 tggaaacaaa gcaggacgac accgccctgt tcctggtcga ggttcagcag gccggcgttt
     1801 tccagatcac cggctacgaa aaggacgagt ttgcgcatgt ggtcggcagc ttctgcccga
     1861 gcgtgctgtt cccgtatgcg cgcgaggcga tcgcgagcgt cgtttctcgt ggtggtttcc
     1921 cggagttcat tttgcagccg atcaactttg atgcgctgta cgcccaaagc ctgcagcagc
     1981 aaaaggacgc ggcgggcgaa gaggcccact aggcccgatg agcgaccggc ccgcgatcgc
     2041 agtaatcggt gccggctcct ggggtaccgc gctcgccctg caactggcgc gcaatgaccg
     2101 gcccgtgcgc ctgtgggggc gcgataagga gcaggtcgcc gcgatgcagc gcgaccgggt
     2161 caacgcgcgc tacctgccag gcgttccatt ccccgacacg ttgcgcgttt tcggcgacat
     2221 taaagagtgc ctggacggtg tgcgcgacat tctgattgtc gtgcccagcc atgcgctgcg
     2281 cgagacgctt aaccgcattc gcccactgct cgccagcgat gcccgaatcg cctgggcaac
     2341 caagggtttc gagctgcaca gcggcaagct gccgcaccag gtggccgccg aggtgctggg
     2401 agaccggcat gcgatggcgg tcctgtcagg ccccacgttc gccaccgagg tcggcgcggg
     2461 cctgcccacc gccttgaccg tggccggcaa tgacgagggt ttcgcccgcg atctcgcgga
     2521 aagcctgtcc ggcgaaaact tccgcgcgta tacgtcgtcg gacgtggtcg gtgtcgaggt
     2581 cggcggcgcg gtcaaaaacg tcctcgcgat tggcgccgga atcaccgacg gactcgggtt
     2641 tggcgcaaat acgcggatcg cgctgatcag ccgcggcctg gtggaaatga cccggctggg
     2701 ggtggcgctt ggcgcacagg ccgacacatt ccgtggcctg gccggcatgg gtgatcttgt
     2761 gctgacctgc accgacgatc agtcgcgcaa ccggcgcatg ggtttgcagc ttgccagcgg
     2821 taaaaccatc ggcgaggcgc aggaagccat tcagcaggtg gtcgagggtg tgatggcagc
     2881 ggaggcggtt aacgaggttg cagaggggct gggcgtcgag atgccgatct gcaaggagat
     2941 ctaccgcatc cttt
//
LOCUS       DATCZV010000588         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_100123, whole genome shotgun sequence.
ACCESSION   DATCZV010000588 DATCZV010000000
VERSION     DATCZV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100123"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VIW27_13285"
     CDS             complement(<1..557)
                     /locus_tag="VIW27_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740694.1"
                     /translation="MLELSGVETFYGSIQALFGMNLRCEEGRMTTLIGRNGMGKTTTV
                     GSIIGTLGCRGEIRFDGEPVSGKPAYEIAQRGIGLVPEGRQVFPNLTVRENLIATAAN
                     YSGSDDPWTLDKVLDFFPRLAERLDNSGSQLSGGEQQMLAIGRALMTNPKLLILDEAT
                     EGLAPLIRAEIWRVLEDLKRAGQSIL"
     gene            complement(550..1302)
                     /locus_tag="VIW27_13290"
     CDS             complement(550..1302)
                     /locus_tag="VIW27_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007427914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740695.1"
                     /translation="MADNLLVVSALHKHFGGVVATDNVNLEVEQGRIHAIIGPNGAGK
                     TTLIAQLSGQLRPDSGDIRFAGHSIVGYSTARRAREGLARSFQITSVVLQMTLLENVM
                     LAVQGTGGHSFRFWSPVSRDRGMREAALASLEQVGLAERAGQLAGNVSHGEQRQLEVA
                     MALAMKPRMLLLDEPMAGMGKEEGAMMVEILGRLRGDKTILLVEHDMDAVFSLADQLS
                     VLVDGHIIATDTVDRIRDNVEVQRAYLGDSHA"
     gene            complement(1292..2263)
                     /locus_tag="VIW27_13295"
     CDS             complement(1292..2263)
                     /locus_tag="VIW27_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5740696.1"
                     /translation="MTAWLPSSVSGRIYAALLLLLAVAPFLLRWLDQPFYLDVLSRGL
                     ILAIAVVSLNLILGYGGMVSLGHAAYIGIGAYSVGIPAYYDYYSGWLHLALALGVGGL
                     FALITGAICLRTKGVYFIMITMAFSQMIFFIFLSLEEYGADDGLVIYLRSEFPAWISM
                     DSAFGLYYWILAILLLALFFIHRLVHARFGRVIVGSKFNEQRMQALGFDTYRYRLACY
                     VLSAMLCSVAGMLLGNFAGFISPDIMSWPRSGELIFMVIIGGVGTLFGPLVGTIAFVL
                     LEEFLSAITVYWHLIFGVMLVALVLYGKGGIHGWLSLLDRREGGHGR"
     gene            complement(2260..>2583)
                     /locus_tag="VIW27_13300"
     CDS             complement(2260..>2583)
                     /locus_tag="VIW27_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5740697.1"
                     /translation="MAGFAGMLIAPITEASIGMGNDIIIVAFVVVIVGGIGSVKGAFY
                     AALILGMLDTMSRSYLDVVLQLFMPVNYAETAAPALSAMLIYVLMAAVLAFRPQGLFP
                     PAGAK"
BASE COUNT          512 a          841 c          815 g          415 t
ORIGIN      
        1 aggatggatt ggccggcgcg cttcaggtcc tccagcaccc gccagatctc ggcccggatc
       61 aagggcgcta ggccctccgt cgcctcgtcc aggatcagga gcttggggtt ggtcatgagc
      121 gcgcggccga tcgccagcat ctgctgttcg ccgccggaca gctggctgcc ggagttgtcg
      181 aggcgctccg ccagccgcgg aaagaaatcg agcaccttgt cgagcgtcca cggatcgtcg
      241 ctgccgctgt agttggcggc ggtagcgatc aggttttcgc gcaccgtcag gttcggaaac
      301 acctgccgcc cctcgggcac caggccgatg ccgcgctggg cgatctcgta agccggcttg
      361 ccgctgaccg gttcgccgtc gaagcggatt tcgccgcggc agccgagcgt gccgatgatc
      421 gagccgacgg tcgtcgtctt gcccatgccg ttgcgcccga tcagcgtcgt catgcgtccc
      481 tcctcgcaac gcaggttcat gccgaacagg gcctggatgc tgccgtagaa ggtctccacg
      541 cccgacagtt caagcatggc tgtctccgag gtaggctctt tgtacctcga cgttgtcacg
      601 aatcctgtcc accgtgtcgg tggcgatgat atgcccgtcg accagcaccg acagctggtc
      661 ggcgaggctg aacaccgcat ccatgtcgtg ctcgaccagc aggatcgtct tgtcgccgcg
      721 cagcctgccg aggatctcga ccatcatcgc gccctcttcc ttgcccatgc cggccatcgg
      781 ttcgtcgagc agcagcatgc gcggtttcat cgccagcgcc atcgccacct cgagctggcg
      841 ctgctcgccg tgggaaacgt tgcccgcgag ttgtccggcg cgctcggcga ggccgacctg
      901 ctcgagactc gcgagtgccg cctcgcgcat gccgcggtcg cgcgagaccg gcgaccagaa
      961 gcggaacgaa tggccgccgg tgccctgcac cgcaagcatg acgttttcca gcagcgtcat
     1021 ctgcagcacc acgctggtga tctggaacga gcgcgccaga ccctcgcggg cgcgccgcgc
     1081 ggtgctgtag ccgacgatac tgtgaccggc gaagcgaatg tcgccgctgt cgggacgcaa
     1141 ttgcccggac agctgcgcga tcagcgtcgt cttgccggcg ccattggggc cgatgatggc
     1201 gtggattctg ccctgctcga cttcgaggtt gacgttgtcg gtcgccacca cgccgccgaa
     1261 gtgcttgtgc aatgcagaca ccaccagcag gttatcggcc atgcccgccc tcccgccgat
     1321 cgagcaggct cagccagccg tgaatgccgc ccttgccgta gagcaccagc gccaccagca
     1381 tgacgccgaa gatcaggtgc cagtaaaccg tgatcgccga tagaaactcc tccagcagca
     1441 cgaaagcgat ggtgcccacc agcggcccga acagggtgcc gacaccgccg atgatgacca
     1501 tgaagatcag ttcgccggaa cgcggccagc tcatgatgtc ggggctgatg aaaccggcga
     1561 agttgccgag cagcatgccg gcgacgctgc acagcatcgc cgacagcacg tagcaggcca
     1621 gccggtaacg gtaggtgtcg aagcccagtg cctgcatgcg ctgctcgttg aacttcgaac
     1681 cgacgattac gcggccgaaa cgcgcgtgca ccaggcgatg gataaaaaac agtgccagca
     1741 gcaggatcgc gagaatccag taataaagac cgaaggcgct atccatgctg atccaggcgg
     1801 gaaactcgct gcgcagatag atcaccaggc cgtcgtcggc gccgtactcc tcgaggctga
     1861 gaaatataaa aaagatcatc tggctgaagg ccatggtgat catgatgaag taaacgccct
     1921 tcgtgcgcag gcagatcgcc ccggtgatca gcgcgaacag gccgccgacg ccgagcgcca
     1981 gcgcgaggtg caaccagcca ctgtagtagt cgtagtaggc cggaatgccg acgctgtagg
     2041 cgccgatgcc gatataggcg gcgtgaccga ggctgaccat gccgccgtaa ccgaggatca
     2101 ggttgaggct gacgaccgcg atggcgagga tcagcccgcg gctgagcacg tcgaggtaaa
     2161 agggttgatc gagccagcgc agcagaaacg gcgccaccgc cagcaacagc agcagggccg
     2221 cgtagatacg tccgctaacg ctgctcggca gccaggccgt cacttggcac ccgccggcgg
     2281 gaacaggccc tgtggccgaa acgcgagcac cgcggccatc agtacataga tcagcatcgc
     2341 cgacagggcc ggcgcggcgg tttcggcata gttcaccggc atgaacagct gcagcacgac
     2401 gtcgagatag gatctcgaca ttgtatccag catgccgagg atcagcgcgg cgtaaaacgc
     2461 acccttgacc gagccgatgc cgccgacgat tacaacgaca aaggcgacga taatgatatc
     2521 gttacccatg ccgatactgg cctcggtgat cggcgcgatc aacataccgg caaaaccggc
     2581 cat
//
LOCUS       DATCZV010000589         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_523850, whole genome shotgun sequence.
ACCESSION   DATCZV010000589 DATCZV010000000
VERSION     DATCZV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523850"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VIW27_13305"
     CDS             complement(<1..1085)
                     /locus_tag="VIW27_13305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010439595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5740698.1"
                     /translation="MKSHARVVVIGGGVVGCSVIYHLTRLGMQDVLLIERSELTSGST
                     WHAAGGFHTLNADTNMAALQGYTIRLYRELEEITGQSCSLHHVGGITLAESPERLDFL
                     KSARAMHRHMGLDTELITPDEIRNLSPITNTDGVLGALYDPLDGHLDPSGTTHAYAKA
                     AQMQGAEILLRNPVLETNPRPDGSWEVVTEQGTIIAEQLVNAAGLWAREVGAMAGIYL
                     PLHPMEHQYFATADIGEVCEREQELPHVMDPEGESYLRQEGKGLVIGFYEQNCVPWAV
                     DGTRWDFGHELLDENLDRIGDSMEFAFRRFPVLAEAGIKTIINGPFTFAPDGNPLVGP
                     VPGLRNYWSACAVMAGFSQGGGVGLTLA"
     gene            complement(1091..>2121)
                     /locus_tag="VIW27_13310"
     CDS             complement(1091..>2121)
                     /locus_tag="VIW27_13310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740699.1"
                     /translation="VGSLVMKTAANDYLRPTMALMDRLDSPYSEYSPAEIREKLPVWN
                     TDSFYPVKRPQDPGFGVPNDEPLPGGVLFHAAGYISDPQLATHNIMRAAEIAGGEFRY
                     NARVVEILRAGGKVSGIALENGETLESPVVLNAAGPHSSKVNQMADVEKSMKVKTRAL
                     RQEVAHVPLPEGYRRETACVTSDSDIGLYTRPEQGDALLIGSEDPPCDPHEWVDPDDY
                     NRDFSEQWKVQVLRTCQRVPNLGVPNQMRGVVDLYDVTDDWIPIYDQSDLPGFYLAIG
                     TSGNQFKNAPIAGQLMADIIEATQNGNDQDASPIDFHLKYIDHTFSSGFYSRLREINQ
                     DSSFSVLG"
BASE COUNT          396 a          654 c          653 g          418 t
ORIGIN      
        1 gccagcgtca ggccaacccc gccgccctgg ctgaatcccg ccataacggc gcaggccgac
       61 cagtagttac gcaacccggg taccggcccg accagcggat taccgtccgg cgcaaaggta
      121 aagggcccgt tgataatggt cttgatgccg gcttcggcga gcaccggaaa acgccgaaac
      181 gcaaattcca tcgaatcgcc gattcgatcg agattttcgt ccagcagctc gtgcccgaaa
      241 tcccaacgcg tgccgtcgac cgcccagggg acgcagttct gttcgtaaaa cccgatcacc
      301 agtcccttgc cttcctggcg caggtaactc tcgccctcgg gatccatcac gtgcggtagc
      361 tcctgttcgc gctcgcagac ttcgccgatg tcggcggttg caaaatactg gtgctccatc
      421 gggtgcagcg gcaggtaaat cccggccatg gcgccgacct cgcgtgccca caggccggcg
      481 gcattgacca gctgctcggc gatgatggtg ccctgctcgg tgaccacttc ccagctgccg
      541 tcggggcgcg gattggtttc cagcaccggg ttgcgcagca ggatttcggc gccctgcatc
      601 tgtgcggctt tggcataggc atgggtagtc ccggacgggt cgagatgacc atccagcgga
      661 tcgtaaagtg cgccgagcac cccgtcggta ttggtaatag ggctcaagtt gcgaatttcg
      721 tcgggtgtaa tcagctccgt atcgagaccc atgtggcgat gcatggcgcg cgccgacttg
      781 aggaaatcga gccgctccgg cgattccgcg agcgtgatgc cgccgacgtg atgcaggctg
      841 caggactggc cggtgatttc ttcgagctcc cggtataggc gtatggtgta cccctgcagg
      901 gccgccatgt tggtatcggc attcagggtg tgaaatccac ccgcggcatg ccaggtcgag
      961 cccgaggtca actcggagcg ctcgatcaac agcacgtcct gcataccgag cctggtcagg
     1021 tgatagatga cgctacagcc gacaacgcca ccgccgataa cgactacccg tgcatgcgat
     1081 ttcataagcg ttatcccagt accgaaaagc tgctgtcctg gttgatctcg cgcaacctgg
     1141 agtaaaaacc gctggaaaaa gtatgatcga tgtatttcag gtggaaatcg atcgggcttg
     1201 catcctggtc gttaccgttc tgcgtcgcct cgatgatgtc cgccatcagc tggcccgcga
     1261 tcggggcatt cttgaactgg ttgccgctgg tgccgattgc gaggtaaaag ccgggcaggt
     1321 ccgattgatc gtaaatcggg atccagtcgt cggtgacgtc gtacagatcg actacgccgc
     1381 gcatctggtt ggggacgccg agattcggca cgcgctggca ggtgcgcagc acctgcacct
     1441 tccattgttc gctgaaatca cggttgtaat cgtccggatc cacccattcg tgcggatcgc
     1501 agggcggatc ctcgctgccg atcaacaacg cgtcgccctg ctccggccgg gtgtatagcc
     1561 cgatgtcgct atccgacgtg acgcaggcgg tttcgcggcg atagccctcc ggcagtggca
     1621 cgtgagcgac ttcctggcgc agggcgcgag ttttgacctt catcgatttc tcaacatcgg
     1681 ccatctggtt gaccttggac gaatgcggac cggcggcatt gagcaccacc ggcgactcga
     1741 gggtttcgcc attctcgagg gcgataccgc tgaccttgcc cccggcgcgc aggatctcga
     1801 cgacccgggc gttgtagcga aactcacctc cggcaatctc ggcggcgcgc atgatgttat
     1861 gcgtcgcaag ctgcggatcg gaaatgtaac cggcggcatg gaacaacacc ccgcccggca
     1921 gcggctcatc gttgggcacg ccgaagccgg gatcctgcgg acgcttgacc gggtaaaacg
     1981 agtcggtatt ccagaccggc agcttctcgc gaatttccgc gggcgagtac tcgctgtagg
     2041 gcgaatcgag ccggtccatc aacgccatgg tcgggcgcag gtagtcgttg gccgcggttt
     2101 tcatcaccag cgaaccgacg t
//
LOCUS       DATCZV010000590         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_259818, whole genome shotgun sequence.
ACCESSION   DATCZV010000590 DATCZV010000000
VERSION     DATCZV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259818"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VIW27_13315"
     CDS             <1..613
                     /locus_tag="VIW27_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007824865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5740700.1"
                     /translation="MDPTVPIEDTVGAMAQLVEQGKVGAIGLSEAGAQTIRRAQATHP
                     LAAIQTEYSLATRDVEAEILPTCRELGIGFVAYAPLSRGLLSGQVTSLDQLGENDRRR
                     DMPRFQGENLGNNIAMVKAVSEIAADKGVSTAALALAWVLAQGKDVVPIPGCRRRETL
                     ADSMTALEVTLSADELERITVALSAERIVGTRYPEKQMARLGL"
     gene            643..1569
                     /locus_tag="VIW27_13320"
     CDS             643..1569
                     /locus_tag="VIW27_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5740701.1"
                     /translation="MNDSAENYDIRSEDHWTKKGDVRLFMYEKYADSPEDKRGTVLFV
                     HGSSMASQPTFDLQVPGRPFSSVMDWFAARGFDTWCVDMEGYGRSDKHRDITCDIANG
                     ADDLAAASEYIQQLRGNDGFLVYGISSGALRAAMFAANHPERIARLALDAFVWTGEGS
                     PTLAERKKKLPQFLDSKRRPIDRDFVYSIFNRDHPGTAADDVIDAFADAITALDDSMP
                     NGTYIDMCSRLPLVQPEQITCPTIVMRGEYDGIAGFGDLVAFFTALPNNDKQFCVMPG
                     ISHASFQQKNYLMVYHILHAFFIQPEPLYRGE"
     gene            1572..2453
                     /locus_tag="VIW27_13325"
     CDS             1572..2453
                     /locus_tag="VIW27_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5740702.1"
                     /translation="MKIATFDYQGQRGVGRVDEVAGTIARFELTAEQANEGIQCLIEM
                     QVAGKDLPPTAEPLELTAVRLEAPLPRPRRNIFCVGKNYFDHAHEFAGSGFDSSAARG
                     AVPEFPIVFSKVPESVVGNGADIEFDPAVSSAIDYEVELAVIIGKPGRGIRSDNALDH
                     VWGYTIVNDVTARDLQGRYSQWLIGKSQDTFCPMGPVAVSRDEIDLADCGVRSFVNDE
                     KRQDSNTGLLIFDVPTIIETIAAGITLLPGDVIATGTPAGVGIGFKPPKYMGDGDLVR
                     CEIDGIGALENRLRRRG"
BASE COUNT          499 a          843 c          822 g          446 t
ORIGIN      
        1 catggacccg acggtgccga tcgaggatac cgtcggcgcg atggcgcagc tggtcgagca
       61 gggcaaggtc ggcgccattg gcctgtccga ggccggcgcg caaaccattc gccgcgcgca
      121 ggcgacccat ccgctggccg cgatccagac cgaatattcg ctcgccaccc gcgacgtcga
      181 ggcggaaatc ctgccgacct gccgcgaact cggcatcggc ttcgtcgcct acgcgccgtt
      241 gagtcgcggc ctgctgagcg ggcaggtcac ctcgctagac caactcggcg agaacgaccg
      301 gcgccgcgac atgccgcgct tccagggcga aaacctcggc aacaacatag cgatggtgaa
      361 agcggtgtcc gaaatcgccg ccgacaaggg tgtgtcaacc gcggcgctgg cgctcgcctg
      421 ggtgctggcg caaggtaagg acgtggtgcc gatcccgggt tgccgccggc gcgagacgct
      481 ggccgattcg atgaccgcgc tcgaggtgac gctgtccgcc gacgagctgg aacgcatcac
      541 cgtcgcgttg tccgccgagc gcatcgtcgg cacgcgctat cccgagaagc agatggcgcg
      601 tctcggactg taaaacgaat tttcaaaccc acggaaactc ccatgaacga cagcgctgaa
      661 aactacgaca ttcgaagcga ggaccactgg acgaagaagg gcgacgtgcg cctgtttatg
      721 tacgaaaagt acgccgattc gcccgaggac aagcgcggca cggtgctgtt cgtgcacggt
      781 tcgtcgatgg cttcgcagcc gaccttcgac ctgcaggtgc cggggcgacc tttcagctcg
      841 gtgatggact ggttcgcggc gcgtggcttc gatacctggt gcgtggatat ggaaggctac
      901 ggtcgctccg acaagcaccg cgacatcacc tgcgacatcg ccaatggtgc cgacgacctg
      961 gccgccgcca gcgagtacat tcaacaattg cgtggcaacg acggtttcct ggtctacggt
     1021 atctcgtcgg gagccttgcg cgcggcaatg ttcgccgcca atcatcccga gcgcatcgcg
     1081 cggctggcgc tcgacgcctt cgtgtggacc ggcgagggca gcccgacact cgccgagcgc
     1141 aagaagaaac tgccgcagtt cctggattcg aaacgccggc cgatcgaccg cgatttcgtc
     1201 tactcgatct tcaatcgcga ccatcccggc accgccgccg atgatgtcat cgacgcgttt
     1261 gccgacgcga tcaccgcgct cgacgactcg atgcccaacg gcacctatat cgacatgtgc
     1321 tcgcgcctgc cgctggtgca gccggagcag atcacctgcc cgacgatcgt catgcgcggc
     1381 gagtacgatg gcatcgccgg tttcggcgac ctggtcgcgt tcttcaccgc gctgcccaac
     1441 aacgacaagc agttctgcgt gatgccgggg atctcgcacg cgagctttca gcaaaagaat
     1501 tacctgatgg tctatcatat cctgcatgcc tttttcatcc agcccgaacc cctgtatcgc
     1561 ggagaatagc cgtgaagatc gcaacctttg attaccaggg gcagcgtggc gtcggccgcg
     1621 tcgacgaggt cgccggcacc attgcccggt tcgagctgac cgccgaacag gcgaacgagg
     1681 gtatccagtg cctgatcgaa atgcaggtcg ccggcaaaga cctgccgccg acggcggagc
     1741 ccctcgagct caccgcggtc aggctcgagg ccccgctgcc gcggccgcgg cgcaatattt
     1801 tctgcgtcgg caagaattat ttcgaccacg cgcacgagtt cgccggcagc ggtttcgatt
     1861 cgagcgccgc caggggtgcg gtgccggagt tcccgatcgt gttttccaag gtacccgaat
     1921 cggtcgtcgg caatggcgcc gatatcgagt ttgatccggc ggtgtcgagc gcgatcgatt
     1981 acgaggtcga gctggcggtg atcatcggca agccggggcg cggcatcagg agcgacaatg
     2041 cgctcgacca cgtctggggc tacaccatcg tcaacgacgt caccgcgcgc gacctgcagg
     2101 ggcgttacag ccagtggctg atcggcaagt cgcaggatac cttttgtccg atggggccgg
     2161 tggccgtgag ccgcgacgaa atcgacctcg ccgactgcgg cgtgcgcagc ttcgtcaacg
     2221 acgaaaagcg ccaggattcg aataccggcc tgctgatctt cgacgtgccg acgatcatcg
     2281 aaaccatcgc cgccggcatc acgctactgc ccggcgacgt gatcgccacc ggtaccccgg
     2341 ccggcgtcgg catcggtttc aagccgccga aatacatggg cgacggtgat ctggttcgct
     2401 gcgaaatcga tggcatcggc gcgctcgaaa accgcctgcg gcgccgcggc tgaatggcac
     2461 gagatactcc ggcgataagc agtgcctgag cgcagccagg ccttgacgtt gcataccggt
     2521 cgattaatcg agtagcaggc accccgaaac cttgaagata agtctgcgca tactgttcca
     2581 gcaggacacg attcgcccgc tgctgaagac
//
LOCUS       DATCZV010000591         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_100432, whole genome shotgun sequence.
ACCESSION   DATCZV010000591 DATCZV010000000
VERSION     DATCZV010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100432"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VIW27_13330"
     CDS             complement(<1..973)
                     /locus_tag="VIW27_13330"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016961470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEY5740703.1"
                     /translation="MHLARFPRLHFAHLPTPLEPLPRLSEELGGPHIWIKRDDCTGLS
                     SGGNKTRKLEFLMADAIAREADCIVTQGAIQSNHARQTSAIAARLDLECHLLLEDRTG
                     YHDEAYVHGGNVLLDQLHGATLSTRPAGSDMDAELVLFADRLREQGRNPYVIPGGGSN
                     EIGALGYVNAALELVAQANERSLRIDHLVHATGSCGTQAGLVVGMEAMHSGIPIYGVC
                     VSRGSEEQQDKVYQLVQRTSEYLDLGANFVDRERVITNGDYFGDGYGLPTEGMIEAVK
                     MLASYEGILLDPVYAGKGFAGLLDLVRQGHFKAGENVVFLHTGGSVSL"
     gene            1188..>2133
                     /locus_tag="VIW27_13335"
     CDS             1188..>2133
                     /locus_tag="VIW27_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5740704.1"
                     /translation="MTPGFLLTRQWADDRDGLRLDFWLATARGPLSVSIHRQQALFFI
                     RQSEQQTAGEVLRGMPGVAMRPLQLKTFAEAPVSAVYFDSQQQLYRARDRLTAAGITC
                     IEADIRPTERFLSERFITASIDVDADYGTSTLHDVRLQPGEYIPRLDVMSLDIESDFE
                     TDELFSIAFVTAELRRAIVIGTGETAEDIEYVADEAALLDRWIDWVERVDPDVFIGWN
                     VINFDLRLLQKRALATGRALAIGRGGALPAWRQSQQDAGQHFVLVPGRVVLDGIDTLR
                     SATYNFASFALEAVARELLGRGKLIDSTPRADPLFKARE"
BASE COUNT          353 a          671 c          703 g          406 t
ORIGIN      
        1 acaacgacac gctgccgccg gtatgcagga aaaccacgtt ttctcctgcc ttgaaatgac
       61 cctgcctgac caggtcgagc aggcccgcaa aacccttgcc ggcgtaaacc gggtcgagca
      121 ggatgccctc gtagctggcg agcatcttca ccgcttcgat catgccctcg gtgggcaggc
      181 cgtaaccgtc gccgaagtag tcgccattgg tgattacccg ttcgcggtcg acgaagttcg
      241 cgcccaggtc gaggtactcg ctggtgcgtt gcaccagctg gtaaaccttg tcctgctgct
      301 cctcgctgcc gcggctgacg caaacgccgt agatcggaat gccgctgtgc atcgcctcca
      361 tgccgaccac caggccggcc tgggtgccgc agctgccggt cgcgtgcacc aggtggtcga
      421 tgcgcagcga acgctcgttg gcctgggcga cgagctccag tgccgcgttg acataaccca
      481 gcgcgccgat ctcgttggag ccgccacccg gaatcacgta gggattgcga ccttgctcgc
      541 gcagccggtc cgcgaacaac accagctcgg cgtccatgtc gctgcccgcg gggcgcgtcg
      601 acagcgtggc gccgtgtagc tgatcgagca gcacgttgcc gccgtgcaca taggcttcgt
      661 cgtggtaacc ggtacgatcc tcgagcaaca ggtggcattc gaggtcgagc cgcgcggcga
      721 tcgcgctggt ctggcgcgca tggttggact ggattgcgcc ctgggtaaca atgcagtcgg
      781 cttcgcgggc gatggcatcg gccatcagga attccagctt gcgcgtcttg ttgccgccgg
      841 acgacaggcc ggtacagtcg tctcgcttga tccagatgtg tggcccgccg agttcctcgg
      901 acaggcgcgg caggggttcc agcggggtgg gcaggtgcgc gaagtgcagg cgcggaaagc
      961 gggccagatg catgacggac tccggtacag ggtgccggtt cgaggccggc gcgtttcacg
     1021 gcagtaatgc ggacaattta aagtaaaatg actgtcgcgt atattcccgg acccgaaaaa
     1081 cctggtttga aattcattcg gctgcagttt ccgctatcga ttcgatcgct tcccggcatt
     1141 taccggcggg cgatttgccg taccgacccg gacgggagcg catcgccttg acgccgggat
     1201 tcctgctgac gcgccagtgg gccgacgacc gcgatggcct gcgcctcgat ttctggctgg
     1261 cgaccgcgcg cgggccgctg tcggtatcga ttcaccgtca gcaggcgctg ttctttattc
     1321 gccagtccga gcagcaaacg gcgggcgagg tattgcgcgg catgcccggc gtagcgatgc
     1381 ggccgctgca attgaagact tttgccgagg cgccagtcag cgcggtgtat ttcgattcgc
     1441 agcagcagct gtaccgcgcg cgcgaccgtc tcacggccgc cggaatcacc tgcatcgagg
     1501 ccgatattcg gcccaccgag cgcttcctga gcgagcgttt catcaccgcc tcgatcgacg
     1561 tcgacgcgga ttacggcacc tccaccctgc acgatgtgcg cctgcagccc ggtgaataca
     1621 ttccacggct cgacgttatg tccctggaca tcgaatccga cttcgaaacc gacgagttgt
     1681 tttcgattgc cttcgtcacc gcggagctgc ggcgtgcgat cgttatcggc acgggcgaga
     1741 cggccgagga tatcgagtac gtcgccgacg aggcggcttt gctggaccgc tggatcgact
     1801 gggtagagcg cgtcgacccg gacgtcttca tcggctggaa cgtgatcaat ttcgatctgc
     1861 ggctgttgca gaagcgagcg ctggcgacgg gacgggcgtt ggccatcggc cgcggcggcg
     1921 cgctgccggc gtggcggcag tcgcagcagg atgcgggcca gcatttcgtg ctggtcccgg
     1981 gtcgggtggt gctcgacggc atcgatacgc tgcgctcggc aacctataat ttcgccagct
     2041 tcgcgctcga ggcggtggcc cgcgaactgc tcggccgcgg taaactgatc gattcgactc
     2101 cgcgggccga tccgcttttc aaggcgcgcg aga
//
LOCUS       DATCZV010000592         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_736486, whole genome shotgun sequence.
ACCESSION   DATCZV010000592 DATCZV010000000
VERSION     DATCZV010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736486"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VIW27_13340"
     CDS             <1..1020
                     /locus_tag="VIW27_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5740705.1"
                     /translation="GRRAVRALVVTPTRELAAQVAQSIDDYGKWLPLKSAVVFGGVSI
                     NPQKQKLIRGVDILVATPGRLLDHAGQRSVDLSRVELLVLDEADRMLDMGFIHDIRRI
                     LKLLPQQKQTLLFSATFSDEIRKLASGLLKSPATVEVARRNATVDAIEQIVHPVEKAR
                     KRELLSFLIGSNNWRQVLVFGRTKHGANRLAKQLLSDGIETAAIHGNKSQGARTRALA
                     DFKSGKLRVLVATDIAARGLDIDQLARVVNFELPNVAEDYVHRIGRTGRAGREGQAIS
                     LVSPDEVKQLRDIQRLINREIPTRAIAGYHAGTALENKAFDSAPQAPKRNRRRRASSS
                     RRAAA"
     gene            1045..1299
                     /locus_tag="VIW27_13345"
     CDS             1045..1299
                     /locus_tag="VIW27_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740706.1"
                     /translation="MTEKSDETPDRHFEVTSIEKTEPPADQTRGDWYQYTIGEGDSAI
                     TGKRAGSLASVRRHAEEFAENLNKRAALGYSAYATRRSQK"
     gene            1388..1786
                     /locus_tag="VIW27_13350"
     CDS             1388..1786
                     /locus_tag="VIW27_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740707.1"
                     /translation="MNGFDEIRISGVDDTRPPRLRKEGYIDLYFTLSRQVPEEWCEDF
                     NAIGRRISPSAKIDKNSCLTIDTYVNDMDRIVAQLAELKEAVGDCNRQYAEKLQQREL
                     ALAAENASLQGEGGEQQRLNSIIAALEFDD"
     gene            1892..>2178
                     /locus_tag="VIW27_13355"
     CDS             1892..>2178
                     /locus_tag="VIW27_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY5740708.1"
                     /translation="MSKKAPEKTGVYLKRFINGVKKRNPGEKEFHQAVSEVAATIFPY
                     IADKPIYHKMQIMERMAEPDRVVMFRVPWTDDKGNIRNQRGYRVQFNNSIGP"
BASE COUNT          510 a          688 c          638 g          342 t
ORIGIN      
        1 ggccgccgcg cggtgcgcgc actggtggtc accccgaccc gcgagctggc cgctcaggtc
       61 gcacagagca tcgacgatta cggcaaatgg ctgccgctca aatcggccgt cgtattcggc
      121 ggcgtcagca tcaacccgca aaaacagaaa ctgattcgcg gggtcgatat cctggtcgca
      181 acccccggcc gcctgctgga ccacgcgggc cagcgatcgg tggacctgtc gcgagtcgag
      241 ctgctggtgc tggacgaggc cgaccgcatg ctcgacatgg gtttcattca cgatattcgc
      301 cgcatcctga aacttttgcc gcaacaaaaa caaaccctgc tgttctcggc gaccttctcc
      361 gacgaaatca ggaagctcgc cagcggtttg ctgaaatccc cggcgaccgt ggaagtcgcg
      421 cgccgcaatg ccaccgtgga tgcgatcgaa caaatcgtgc acccggtcga gaaggcgcgc
      481 aaacgcgaac tgctaagctt cctgatcggc tccaataact ggcgccaggt gctggtattc
      541 ggccgcacca agcacggtgc caaccggctg gcgaaacaac tgctcagcga cggcatcgaa
      601 accgcggcga ttcatggcaa caagagccag ggcgcgcgga cgcgcgcgtt ggccgacttc
      661 aagtccggca agctgcgggt gctggtggcg accgatatcg ccgcacgcgg actggatatc
      721 gaccagctcg cccgagtcgt caacttcgaa ttacccaatg tcgccgagga ctacgtacac
      781 cgcatcggcc gcaccggtcg tgccggacgc gagggccagg ccatctccct ggtgagcccg
      841 gacgaggtca agcagctcag ggatatccaa agactgatca acagagagat tcccaccagg
      901 gcgattgcgg gttaccatgc ggggacggcc ctggaaaaca aggcgtttga ttccgcaccg
      961 caggcaccga aacgcaaccg gcgccggcga gcttcttcca gtcgacgcgc ggcagcctga
     1021 tccaggcagg aaacgtaagc agcgatgaca gagaaatccg acgagacccc agatcgccat
     1081 ttcgaggtta cgagcatcga gaaaaccgag cccccggccg accagaccag gggcgactgg
     1141 taccagtaca ctatcggcga gggtgattcg gcgatcaccg gcaagcgcgc cggcagcctc
     1201 gcctcggtgc gcaggcacgc cgaggaattc gctgaaaatc tcaacaagcg cgcggccctc
     1261 ggctactcgg cctacgccac gcgccgctcg cagaagtaga cgggcctgaa cgcaaccacg
     1321 ccgacgcgtc cgccacccgc cgaaattttc ggccggcgcg gcgcgccgaa taccggagta
     1381 gtgcccaatg aacggtttcg atgaaatccg catcagcggt gtcgacgaca cccgcccgcc
     1441 gaggctccgc aaggaaggct atatcgacct gtatttcacg ctttcgcgcc aggttcccga
     1501 ggagtggtgc gaggacttca acgccatcgg ccgccgcatc agcccgtcag ccaagatcga
     1561 caagaactcg tgcctgacta tcgacaccta cgtcaacgac atggaccgga tcgtggcaca
     1621 gctcgccgaa ctcaaggaag ccgtcggcga ctgtaaccgc caatatgccg aaaagctgca
     1681 acaacgcgaa ctggcgctgg ccgcggagaa tgcgtcgcta cagggcgaag gcggcgagca
     1741 acaacggctc aactcgatca tcgccgcact cgagttcgac gactgaagcc ccctcctggc
     1801 tggaaacagc cgcattgtcg gcgttaaaat tcaatcgatt ctgccggtat tagcctgtaa
     1861 agtcgtcaaa aaagaaattc gaggagggct tatgagcaaa aaagcgcccg aaaaaaccgg
     1921 ggtttacctg aagcgtttca tcaacggcgt caaaaagcga aatccgggag aaaaggaatt
     1981 ccaccaggcc gtatcggaag tcgcggcaac gatttttccg tatatcgccg acaaaccgat
     2041 ttatcacaag atgcaaatca tggagcgcat ggccgaaccc gaccgcgtgg tcatgtttcg
     2101 cgtgccctgg accgatgaca agggcaatat tcgcaaccag cgcggttacc gggtgcagtt
     2161 caataattcg atcggccc
//
LOCUS       DATCZV010000593         7760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_100560, whole genome shotgun sequence.
ACCESSION   DATCZV010000593 DATCZV010000000
VERSION     DATCZV010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100560"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VIW27_13360"
     CDS             complement(<1..586)
                     /locus_tag="VIW27_13360"
                     /inference="COORDINATES: protein motif:HMM:NF045289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5740709.1"
                     /translation="MTDLDKWLPWLGGEWAWAGQVFIVVLATAFLSFFVKRMLAKMLT
                     HVSRTSTHWDEVIVEAARRPITWAVWLQGLEIAAEITFAETRNPIFSYSDPVRDVGVL
                     LCITWFLISLTRGAERELARDSDHVDQHTAEAVGKLVRLAVIITAALVILQTLGFSIS
                     GVLAMGGIGGIAVGFAAKDLLANFFGAFIVYLDRP"
     gene            complement(586..1638)
                     /gene="nagZ"
                     /locus_tag="VIW27_13365"
     CDS             complement(586..1638)
                     /gene="nagZ"
                     /locus_tag="VIW27_13365"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY5740710.1"
                     /translation="MTLGPVMLDIAGTALDAGDRERLRHPQVGGVILFTRNYRDPEQL
                     AALCAEIHALRDPRLVIAVDQEGGRVQRFRDGFQSLPAMGHLGDLYDEDPRQAIGLAE
                     TFAWVMASELLARGVDLSFAPVLDIGDPVSSVIGDRAFHRDPESICHLANAWIRGMRA
                     AGMEAVGKHFPGHGSVEGDSHHVMPFDRRELVEIEVLDLVPFRRVIATHLAGIMMAHV
                     IYDAVDARAAGYSRYWIETVLRGQLGFEGCVFSDDLSMSGAESVGGYPERAAAALDAG
                     CDILLVCNNPQGAEQVLDSLQGYSNPASQLRMVRMHGQEVEASRFRGADWQSACEELR
                     AFNHRLGQADSGDLFD"
     gene            complement(1635..2120)
                     /gene="ispF"
                     /locus_tag="VIW27_13370"
     CDS             complement(1635..2120)
                     /gene="ispF"
                     /locus_tag="VIW27_13370"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY5740711.1"
                     /translation="MARLRIGHGYDVHAFAAGDGFVLGGVSIDCDKAVIAHSDGDVLI
                     HALCDALLGALGRGDIGGLFPDSDAANRDRDSREFLRETRALLDADGYRLANADITVI
                     AQAPRLAPHIAAMREYLSQDLACESAAINIKATTTEKLGFVGRQEGIAAEAVVLLEGD
                     A"
     gene            complement(2257..3291)
                     /locus_tag="VIW27_13375"
     CDS             complement(2257..3291)
                     /locus_tag="VIW27_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5740712.1"
                     /translation="MFQTTDDERAILRWLQLCLLLVFAMVLLGGATRLTGSGLSMVTW
                     HPTGMLPPLDSGEWQAEFELYQQFPEYQLVNRHMTLDDFKRIFWFEYSHRMLGRLIGL
                     VFVLPFAWFWLRGQLTAQLKPRLAIMLVLGGLQGLLGWYMVKSGLIDNPRVSHYRLTA
                     HLLSAILIYGFILWTIFNLADLRDYRRLADSTVVGWRNGSLGLLALVLLTIASGGFVA
                     GLDAGLIYNSFPLMGGELVPQGIDALTPWYLNPLENRVTIQFNHRWLATLTGLLLLVW
                     YWRGRSRFDDALIVRSFKLVGMMVIIQLILGISTLLLQVPVWLGVLHQAGALLLFSAM
                     LFNAHALSRV"
     gene            4053..4865
                     /locus_tag="VIW27_13380"
     CDS             4053..4865
                     /locus_tag="VIW27_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY5740713.1"
                     /translation="MSAQTLKPEEYRITEEPYYEPVGNEIGIFESAWHNKLAISLRGP
                     TGCGKTRFMEYMAWRLQLPLITVSCHDDLTASDLIGRYLITDNQTVWIDGPLTAAVRH
                     GAICYLDEIVEARADTTVVIHPLADDRRVLPVEKLGEVLEAGPEFCMAISYNPGYQSV
                     LKDLKQSTRQRFVALEFDYPDAELEQRIVQTESGLDAANARLLIRFAGLTRNLKGAGL
                     DEGASTRLLVHAGKLMVSGIEPTEACASAVSQALTDDREMIAAVNELCASVF"
     gene            complement(4893..5384)
                     /locus_tag="VIW27_13385"
     CDS             complement(4893..5384)
                     /locus_tag="VIW27_13385"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5740714.1"
                     /translation="MNDISDALADYRALIDSAEGAHLATLGDAAGPEASYAPCAWHDG
                     DCYLFLSALSSHTANLRRDPRISLMLLQEAGSAPNPFARQRVSLRGKVETVDRESGLS
                     TAALAEFHRRFGEVMRLLESLPDFTLFRIELETGSFVRGFGQAYALEGEKLDRLRHLD
                     PRN"
     gene            5738..6937
                     /gene="dapC"
                     /locus_tag="VIW27_13390"
     CDS             5738..6937
                     /gene="dapC"
                     /locus_tag="VIW27_13390"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY5740715.1"
                     /translation="MNTDLSRLQRYPFEKLAELKQGLEPPAQLDHIALSIGEPRHSSP
                     RFVIDSLIAHLHQLSTYPSTRGIEALRETIAAWISRRFAIPAGLVDPERHVLPVSGTR
                     EALFAIAQTVVERGAPAPLVMLPNPFYQIYEGAALLAGAEPRYLDCLPENGFLPDLDA
                     VSPADWSRCQLIYICSPGNPSGAVMPTPYLQRLIELAERHDFVIASDECYSEIYFDEA
                     APPPGLLGAATQMGNRDFARCLAFHSLSKRSNLPGLRSGFVAGDAQLLQQFLLYRTYH
                     GCAMPPATQTASIAAWSDEAHVVENRRLYREKFDRVLALLSGTLAPLRPAAGFYLWPA
                     TPVDDTEFTRRLFAEFNVTVLPGRYLSREHDGGDPGIDRVRMALVAPLDECVDAAQRI
                     NDLWPTL"
     gene            6949..>7760
                     /gene="dapD"
                     /locus_tag="VIW27_13395"
     CDS             6949..>7760
                     /gene="dapD"
                     /locus_tag="VIW27_13395"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY5740716.1"
                     /translation="MSELQHIIEQAFENRAEINPGNVEPGVKQAVLEAIELLDSGRQR
                     VAEPVDGGWQVNEWLKKAVLLSFRICENEIMPGGFTQYYDKVDSRFDGMSAEQHAASG
                     VRVVPPAVARKGSFVAPGVVMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVERNSVISMGVYVGQSTKIYNRE
                     TGEVSYGRIPEGSVVVSGNLPSADGKYSLYCAVIVKRVDEKTRSKVGINELIR"
BASE COUNT         1527 a         2547 c         2381 g         1305 t
ORIGIN      
        1 atggccggtc gagatagacg atgaacgcac cgaagaagtt ggccagcaaa tccttggccg
       61 cgaaaccaac cgcgatgccg ccgatgccgc ccatcgccaa aacaccggaa atactgaacc
      121 cgagggtttg caggatgacc agcgccgcgg tgatgatcac cgccaggcgc accaacttgc
      181 cgaccgcttc cgcggtgtgc tggtcgacat ggtcactgtc gcgcgccaac tcgcgctcgg
      241 caccgcgcgt caggctgatc aggaaccagg tgatacacaa cagcacgccg acatcgcgca
      301 ccgggtcgga ataggaaaag atcgggttgc gggtctcggc gaaagtgatc tcggcagcga
      361 tttccagccc ctgcagccag acagcccagg taatcggccg cctcgcggct tcgacgataa
      421 cctcgtccca gtgggtgctg gtgcggctaa cgtgcgtcag cattttcgcc agcatgcgct
      481 tgacgaagaa actcaggaac gcggtcgcca gcacgacgat aaatacctgg ccggcccagg
      541 cccactcccc gcccagccag ggcagccact tgtcgagatc agtcatcagt caaacaggtc
      601 tccactatcc gcctgcccga ggcgatggtt gaaggcgcgc aattcctcgc aggcggattg
      661 ccagtcggcg ccccggaaac ggcttgcctc gacctcctgt ccgtgcatgc gcaccatgcg
      721 cagctgcgag gcagggttgc tgtaaccctg cagcgaatcg agcacctgct cggctccctg
      781 cggattgttg cataccagca ggatatcgca gccggcgtcg agcgcggccg ccgcgcgttc
      841 agggtagccg cccacggatt cggcgccgct catgctcagg tcgtcgctga acacgcaacc
      901 ctcgaaaccg agttgcccgc gcagcacggt ctcgatccag tagcgcgaat agccggccgc
      961 acgcgcatcg acggcatcgt atatcacgtg cgccatcatg ataccggcca ggtgggtcgc
     1021 gattacccgc cggaacggca ccaggtccag cacctcgatc tcgaccagct cgcggcggtc
     1081 gaaaggcatg acgtggtgtg aatcgccttc taccgagcca tgacccggaa aatgcttgcc
     1141 caccgcctcc atgccggcag cgcgcatacc tcgaatccag gcgttggcga gatggcagat
     1201 agactccggg tcgcgatgaa atgcacgatc gccgatcacg ctgctgaccg gatcgccgat
     1261 atccagcacc ggcgcgaaac tgaggtcgac gccgcgcgcc agcagctcgg aagccatcac
     1321 ccaggcaaag gtctcggcca gcccaatcgc ctgccgcgga tcctcgtcgt agagatcgcc
     1381 gagatgcccc atcgccggca gcgactgaaa accgtcgcga aagcgttgta ctcgcccgcc
     1441 ttcctggtcg acggcgatca ccagccgcgg atccctgagc gcgtgaatct cggcgcacag
     1501 cgccgccagt tgctcgggat cgcggtaatt gcgggtgaac aggatgacgc cgcccacctg
     1561 cgggtggcga aggcgttcac ggtcgccggc gtcgagcgcg gtgccggcga tgtcgagcat
     1621 taccggaccc agcgtcacgc gtcaccttcc agcaatacca cggcctcggc ggcgatgcct
     1681 tcctggcggc cgacgaaacc gagtttttcc gtagtggtcg ccttgatgtt gatcgccgcg
     1741 gactcgcagg cgagatcctg cgacaggtat tcgcgcatcg ccgcgatatg cggcgccagg
     1801 cgcggcgcct gcgcgatgac cgtgatgtcc gcgttcgcca gccggtagcc gtcggcgtcc
     1861 agcaaggccc gcgtttcgcg caaaaactcg cggctgtcgc gatcacggtt ggcggcatcg
     1921 ctgtcgggga acagcccgcc gatgtcaccg cgccccagtg cgccgagcaa cgcgtcgcac
     1981 agcgcatgga tcaacacatc gccatcggaa tgcgcgatca ccgccttgtc gcagtcgatg
     2041 ctgaccccgc cgagcacgaa accatcgccc gcggcgaacg cgtgcacgtc gtacccgtga
     2101 ccgattctaa gcctggccac tagtgccgga ttcctcgcgg ccggcggcgg ccgccagccg
     2161 agagccgccg gctcccgaaa tgacgatcga taagtgcata gcgcaggtcc tcggcgccgc
     2221 tgaaaaatga gggattataa ccctgctcgg gcacaactac acccggctga gcgcgtgcgc
     2281 attgaacagc atggcgctga acagcagcag tgccccggcc tggtgaagca cgccgagcca
     2341 taccggcacc tgcagcaaca gcgtcgagat gccgagtatc aattgaatga tcaccatcat
     2401 accgaccagc ttgaagctgc gcacgatcag cgcatcgtcg aaccgcgaac gcccgcgcca
     2461 gtaccagacg agcagcaaca gcccggtgag cgtcgccagc cagcgatgat tgaactgtat
     2521 cgtaacccgg ttctcgagcg ggttgagata ccagggcgtg agcgcgtcga taccttgcgg
     2581 taccagttcg ccgcccatca gcggaaagct attgtagatc agcccggcat cgaggccggc
     2641 gacgaatcca cccgaggcga tggtcagcag taccagcgcc agcagaccca gcgacccgtt
     2701 tcgccagccg acaacggttg aatccgccag gcggcggtaa tcgcgcaaat cagcgagatt
     2761 gaagatcgtc cacaggatga agccgtagat caggatcgcg ctgagcagat gcgcggtcaa
     2821 ccggtaatgg ctgacccggg gattgtcgat cagtccgctc ttgaccatgt accagccgag
     2881 caaaccctgc aaaccgccga gcaccagcat gatcgcgagc cgcggtttca gttgcgcggt
     2941 caactgtccg cgcaaccaga accacgcgaa cggcagcacg aacaccagcc cgatcaggcg
     3001 cccgagcata cggtgcgaat attcgaacca gaagattcgc ttgaaatcgt cgagcgtcat
     3061 gtgtcgatta accagctggt attcgggaaa ttgctggtaa agctcgaact ccgcctgcca
     3121 ctcgccgcta tcgagcggcg gcagcatgcc agtcggatgc caggtcacca tcgacaatcc
     3181 cgaaccggtg aggcgcgtcg cgccgccgag cagcaccatc gcgaatacca gcagcaggca
     3241 cagttgcagc cagcgaagaa ttgcgcgttc gtcgtcggtt gtctggaaca tgcgggtcga
     3301 aaactccgaa atgccgtcag ccgcgactca cggccgacgc gggtgggtgc cgccggctgg
     3361 caagcttggc gtctgcgaat tcatagcggc gctattatat cgacagtcgc gcgatattgc
     3421 cttcattgcg tcgccatcgg cgaacgaaaa tccaccgcgc ctgggcgatc gcggcaaagc
     3481 atcccggttg tggcgcctcg tggcgacggt atccagccgc ccgccccgcc ctcggcaaga
     3541 cgttgccagc gttcggcggt gggggatctg gcggtagcgc caggtcggcg gcatcgggaa
     3601 cgatgttgcc gactatcgtc gacacctcga gtgttacgca gcgatcgcag gcggcgcgca
     3661 cagacgaggc tggatcagcg gcgcgtgccc tgcaagcatg gcggggagaa tctggcgcca
     3721 ccggagtcga ggccaaaaaa aaggcgccta tgggcgcctt tctcgtcctc gctgccgtaa
     3781 tcgttattat atgggcagca ctctcctcca gttttcaggc ctggtggccg ctgtttcgaa
     3841 ctgcgcgtag taaaccacag gcggcgccaa gttgccatca caaattgcgc gcagccgacg
     3901 aacggcattc cgggcaacat cgagttccgc agcgcaacag catcgtttat catcgccgcg
     3961 cgggccgggg ttatactatg tttccagcgg cgaccagagc ccgcccaggc gtatcgaaat
     4021 cgtcggcaat tgccgcaata tccaggagtc gaatgtcagc acaaactctc aagcccgagg
     4081 aataccgcat caccgaggag ccctattacg aaccggtcgg caacgaaatc ggaatcttcg
     4141 aatcggcctg gcacaacaag ctggcgatat ccctgcgcgg gccgaccggc tgcggcaaga
     4201 cccgcttcat ggagtacatg gcctggcggc tgcaactgcc gctgatcacg gtatcctgcc
     4261 acgacgacct gaccgcctcc gacctgatcg ggcgctacct gatcaccgat aatcagaccg
     4321 tctggatcga cgggccgctg accgctgcgg tgcgtcatgg cgcgatctgc tatctcgacg
     4381 aaatcgtcga ggcgcgcgcc gataccacgg tggtaatcca tccgctggcc gacgaccggc
     4441 gcgtgctgcc ggtcgagaaa ctcggcgagg tgctcgaggc cggccccgag ttctgcatgg
     4501 cgatctccta caatcccggc taccagagcg tgctgaagga cctcaagcaa tccacccgcc
     4561 agcgcttcgt ggcgctggaa ttcgattacc ccgacgccga actcgagcag cgcatcgtac
     4621 agaccgagtc ggggctcgat gccgccaacg caaggctact gatccgtttc gctggcctga
     4681 cccgcaacct caagggcgcc ggcctcgacg agggcgccag cacccgcttg ctggtgcatg
     4741 ccggcaagct gatggtttcc ggtatcgagc cgaccgaagc ctgcgccagt gccgtttccc
     4801 aggcactgac cgacgaccgc gaaatgatcg ccgcggtcaa cgaactctgc gcctcggtat
     4861 tctgacctgt ccgccgctcg ctgccgcggc gattagttgc gtggatccag gtgccgcaaa
     4921 cggtccagct tttcgccttc gagcgcgtag gcctggccga aaccgcgcac gaaactgccg
     4981 gtttcgagtt cgatgcggaa cagcgtgaaa tcggggagcg actcgagcaa gcgcatgacc
     5041 tcgccgaaac gccggtgaaa ttcggccagc gccgcggtgg acaaaccgct ctcgcggtcg
     5101 acggtttcca ccttgccgcg caggctgaca cgttggcgcg cgaacggatt tggcgcactg
     5161 ccggcctcct gcagcaacat caggctgata cggggatcac gccgcagatt cgcggtatgg
     5221 ctcgacaggg cgctcagaaa caggtagcaa tcgccgtcgt gccaggcaca gggcgcgtaa
     5281 ctggcctcgg ggccggcggc atcgccgagg gtcgccaggt gggcaccctc ggcgctatcg
     5341 atcagcgccc ggtaatcggc cagcgcatcg gaaatgtcat tcatgtttca cctctgcttg
     5401 ctggcgacaa ttctgccacg atgcgcgccg cgccgcactc gcggaacgcc aggcgctggc
     5461 aaccgactgg cggcgtaatc agcccggcgg cccgctggtc atcgagtgga gatcaaaaaa
     5521 aaaccccaac gggtcgatgg ggtctaaaac acatgaggtc tgacatgaag ggtatcgaat
     5581 gcaataaagt atagacagcc tggcgaatcg aattgcgacc tgggtctcaa ttcacgccgg
     5641 cgcagctgcg ctcggtccgg cggctgattc acgctgcgac gactgttatc gtcgccatta
     5701 gcgttatact atcgccccgt tggcccgccg aaagtccatg aataccgatt tatcccgctt
     5761 acagcgttat ccgttcgaaa aactggccga gttgaaacag ggcctcgagc cacccgcgca
     5821 gctcgaccat atcgcgctgt cgatcggcga gccgcggcat tcgtcgccgc gcttcgtgat
     5881 cgacagcctg atcgcccacc tgcaccagct atccacctac ccgagcaccc gcggcatcga
     5941 ggcgctgcgc gagacgatcg cagcctggat cagccggcgc ttcgctatcc ccgccggcct
     6001 cgtcgatccc gagcgccatg tgctgccggt cagcggcacc cgcgaagccc tgttcgcgat
     6061 cgcgcaaacc gtcgtcgagc gcggcgcgcc cgcaccgctg gtaatgctgc cgaacccgtt
     6121 ttaccagatc tacgagggag cggcgctgct cgccggtgcc gagccccgct acctcgattg
     6181 cctgccggaa aacggcttcc tgccggatct cgacgcggtc agcccggctg actggtcgcg
     6241 ctgccagttg atttatattt gcagccccgg caatccgagc ggggcggtga tgccgacacc
     6301 ctatctgcag cgcctgatag aactcgcaga acgccacgat ttcgtcatcg ccagcgacga
     6361 atgttactcg gagatttatt tcgacgaggc cgcgccgccg ccggggttgc tcggcgccgc
     6421 cacgcaaatg ggcaaccggg atttcgcccg ctgcctcgct tttcacagcc tgtcgaaacg
     6481 ttccaacctg cccgggctgc gttcgggatt cgtcgccggg gatgcgcagc tgctgcagca
     6541 gttcctgctt taccgcacct atcacggctg cgcgatgcca ccggcgacac agaccgcgag
     6601 tatcgccgcc tggagcgacg aggcccacgt ggtcgagaac cgccgcctgt accgggagaa
     6661 attcgatcgg gttttagcgc tgctttctgg tacactcgcg ccgctacggc ccgcggccgg
     6721 tttttacctg tggcccgcaa ccccggttga cgacaccgag tttacccgcc gcctgttcgc
     6781 cgagttcaac gtcaccgtgc tccccgggcg ttacctgtcc cgcgagcatg acggcggcga
     6841 tcccggcatc gaccgggtgc gcatggcgct ggtcgcgccg ctcgacgaat gcgtcgatgc
     6901 ggcgcagcgc atcaacgacc tgtggccaac gctttaggag agagaaatat gtcagaactt
     6961 cagcacatca tcgaacaggc gttcgaaaat cgcgctgaaa ttaatcccgg caacgtcgag
     7021 ccgggggtca agcaggcggt actagaggcc atcgagctgc tggattccgg gcgccagcgc
     7081 gtcgccgaac cggtcgacgg cggctggcag gtcaacgagt ggctgaagaa ggccgttctg
     7141 ctgtcgtttc gcatttgcga aaacgaaatc atgccgggcg gttttaccca gtattacgac
     7201 aaggtcgatt cgcgctttga cggcatgtcg gccgaacagc atgcagcgag cggcgtgcgc
     7261 gtggtaccgc cggcggtggc gcgcaagggt tcgttcgtcg cgccgggcgt ggtgatgatg
     7321 ccgagctatg tcaatatcgg cgcctacgtc gacagcggca cgatggtcga cacctgggcg
     7381 acagtcggct cctgtgcgca aatcggcaag aacgtgcatc tgtcgggcgg cgtcggcatc
     7441 ggcggcgtgc tcgaaccgct gcaggcttcg ccgaccatca tcgaggacaa ttgcttcatc
     7501 ggcgcgcgct cggaaatcgt cgagggcgtg atcgtcgagc gcaactcggt gatctcgatg
     7561 ggcgtctatg tcggtcagag caccaagatc tacaaccgcg aaaccggcga ggtcagttac
     7621 gggcgtattc ccgagggctc ggtggtggtc tccggcaacc tgccctcggc cgacggcaag
     7681 tacagcctct actgcgcggt gatcgtcaag cgggtcgacg agaaaacccg cagcaaggtc
     7741 ggcatcaacg aactgatacg
//
LOCUS       DATCZV010000594         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_100626, whole genome shotgun sequence.
ACCESSION   DATCZV010000594 DATCZV010000000
VERSION     DATCZV010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100626"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VIW27_13400"
     CDS             complement(<1..259)
                     /locus_tag="VIW27_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5740717.1"
                     /translation="MQLIDSHCHLDDDRFDARRAAVLRDAADAGIERIVVPATTAARW
                     EKLRQVCAPRAGLYPAYGLHPWFCGEHRREHLDRLDAWLARE"
     gene            complement(271..1161)
                     /locus_tag="VIW27_13405"
     CDS             complement(271..1161)
                     /locus_tag="VIW27_13405"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEY5740718.1"
                     /translation="MSKKHPIVVVTGSSGAGTSTVRKALESIFHRQKIRSVSVEGDSF
                     HRYDRAGMREKMDEARRDGLNFSHFGELANEFDLLERLFRSYGEAGSGRRRFYLHDEE
                     EAKHHSARLGIELGAGEFTPWEEIEPETDLLFYEGLHGLVKTSRVDLTRYADLKVGVV
                     PVVNLEWIQKIHRDSAERGYSKDEVVDNILRRMPDYVKYITPQFSETDINFQRVATVD
                     TSNPFIARDIPTLDESFVVIRFRRPDIRNTDFTNLLSMISGSFMSRRNTIVVPGGKMG
                     MAMEIILTPIIGDLLARRGR"
     gene            1492..>2593
                     /locus_tag="VIW27_13410"
     CDS             1492..>2593
                     /locus_tag="VIW27_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5740719.1"
                     /translation="MKWIAGILFLAFGWQIVGVAPSIEIAWVIGLLIFTIYLFVFEIV
                     NVDETAITIMVLLGLSSLLAPTMGLEAGLVDTAHLFDGFSSNAVISIIAVMIIGAGLD
                     KTGLMSKVAAFILRIGGSSETRIIPIVSGTTGIISSFMQNVGAAALFLPVVSRISARS
                     GLPMSRLLMPMGFTAILGGTVTMVGSSPLILLNDLILTSNVALPSELQMQTFGLFSVT
                     PIGLALVATGILYFVLAGRFVLPAVSGDASPAAAGTSKYLQEVYGVDYMLFEVNVPED
                     SPLVGQTLQDIESINKIRIIAVKSAGAGMRASRGDISRDLDIGPNSALGILCSPEHLR
                     DFVDAYGLKIQKGLRVFSEVLSSAQAGIAEIVI"
BASE COUNT          467 a          804 c          775 g          547 t
ORIGIN      
        1 gctcgcgcgc cagccaggcg tccagccggt cgagatgctc gcgccggtgc tcgccgcaaa
       61 accacgggtg caggccgtag gccggataca gcccggcccg cggcgcgcac acttgcctga
      121 gcttttccca gcgcgcggcg gtggtcgccg gaaccacgat gcgctcgata ccggcgtcgg
      181 cggcgtctcg cagcaccgcc gcgcgccgcg catcgaagcg atcgtcgtcg aggtggcaat
      241 ggctgtcgat cagctgcatg gcgcgcgcgg tcagcggccg cggcgcgcaa gcagatcgcc
      301 gatgatcggc gtcagaataa tttccatcgc cattcccatc ttgccaccgg ggacgacgat
      361 cgtgttgcgg cgagacatga acgatccact gatcatcgac agcaggttag tgaaatcggt
      421 attgcgaata tccggtcggc gaaagcgaat cacgacaaaa ctctcgtcca gcgtcggaat
      481 gtcacgggcg atgaacgggt tcgacgtgtc caccgtggcc acgcgctgaa aattgatatc
      541 ggtttccgag aactgcgggg tgatgtactt cacgtagtcg ggcatgcgtc gcaggatgtt
      601 gtcgacgacc tcgtccttgc tgtagccgcg ctcggcgcta tcgcggtgta tcttttgaat
      661 ccactcgagg ttgaccaccg gtaccacgcc gaccttgaga tccgcgtaac gcgtcaggtc
      721 gacccgcgag gtctttacca gcccgtgcaa tccctcgtag aacagcaggt cggtctcggg
      781 ctcgatttct tcccagggcg tgaattcgcc ggcgccgagc tcgataccga gccgcgcgct
      841 atgatgcttc gcctcttctt cgtcgtgcag gtaaaagcga cgccggccgc tgccggcttc
      901 gccgtaggac ctgaacagcc gctccagcag atcgaactcg ttggccagct cgccgaagtg
      961 gctgaagttg agaccgtcgc ggcgcgcttc gtccattttc tcgcgcatgc cggcgcggtc
     1021 atagcggtga aagctgtcac cctcgacgga aaccgagcga atcttttgcc ggtgaaagat
     1081 cgattccagc gccttcctga cggtcgacgt gcccgccccg gaagatccgg taaccacgac
     1141 gatcgggtgt ttcttcgaca tcgaccaacc tgttgcccag ttttcgttca gcggaattct
     1201 atccgctttc atacaggatc gcgatgaccg tataagccgg ttctatcggc ctgcgctttg
     1261 tgcacttgtt attattgtgt acacttgaca gggtaacctg tctcatctgg taagcgccat
     1321 ttctccctgg cgctgcttga ttcaaacagc aatgcgacgc ctgttgtaca gctcgcggca
     1381 gtggcacccg ggctggataa aagtgtatga ggattcgacc gacacttgag cagactatcg
     1441 agaatattcc gggtcagact taaaaaagtc aaactcaaga agctgcgcag catcaagtgg
     1501 attgccggaa ttctctttct tgcgttcggt tggcaaatcg tcggcgtcgc tcccagcatc
     1561 gagatcgcgt gggtcatcgg gctgctgatc tttaccatct acctgttcgt gttcgaaatc
     1621 gtcaacgtcg acgaaaccgc gatcaccatc atggtattgc tcgggctctc gtcgctgctg
     1681 gcgccgacca tggggctcga agccggactg gtcgacaccg cacacctgtt cgacggtttc
     1741 tcgagtaacg cggtgatttc gatcatcgcg gtaatgatca tcggcgccgg cctcgataag
     1801 accggcctga tgagcaaggt cgccgccttt attctgcgta tcggcggcag ttccgaaacg
     1861 cgcatcattc cgatcgtgtc gggcaccacc ggcatcattt cgtcgttcat gcagaacgtc
     1921 ggcgccgcgg cgctgttcct gccggtcgtc agccgcattt cggcgcgctc ggggctgccg
     1981 atgtcgcgcc tgctgatgcc gatgggcttt accgcgattc tcggcggcac cgtcaccatg
     2041 gtcggctcgt cgccactgat cctgctcaac gacctgatcc tgacctccaa cgtggcgctg
     2101 ccgagcgagc tgcaaatgca gaccttcggc ctgttctcgg tgacgccgat cggcctggcc
     2161 ctggttgcca ccggtattct ctatttcgtg ctcgccggac gattcgtgct gccggcggtg
     2221 tccggcgacg catcgccggc tgccgccggc accagcaagt acctgcagga agtctacggg
     2281 gtcgattaca tgctgttcga ggtcaacgtt cccgaggaca gcccgctggt cgggcagacg
     2341 ctgcaggata tcgagtcgat caacaagatc cgcatcatcg cggtcaagtc ggccggcgcc
     2401 gggatgcgcg cgagccgcgg cgatatatcg cgcgacctcg atatcggtcc caactcggcg
     2461 ctcggcatcc tctgctcgcc cgagcacctg cgcgatttcg tcgacgccta cgggctgaag
     2521 atacagaaag gcttgcgggt gttttccgag gtgctgtcat cggcacaggc ggggatcgcg
     2581 gaaatcgtca tac
//
LOCUS       DATCZV010000595         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_736565, whole genome shotgun sequence.
ACCESSION   DATCZV010000595 DATCZV010000000
VERSION     DATCZV010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736565"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1134
                     /locus_tag="VIW27_13415"
     CDS             337..1134
                     /locus_tag="VIW27_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5740720.1"
                     /translation="MASIFWVEDQSHWIKKFSPVLESEDFDGKPNTLTILNFAEAARQ
                     KIALTDVDSPPDVALLDARMNGNDQAGFSVSNALRKKWPRLPIIYLSEHSGTEIERDA
                     LQQFSATDFIAKHQHNVEQVLCWRIKAVLRQSSIDSDLSSTIDVITSGELTIDLNTWN
                     VYWKGQRLMNPSNTRRPLAPTPRKLLRELVEVSPRPLTAGQVAERLNLDPGKFSYGSY
                     RQHIKTLRRAFDAVESGFSETCNRGEGIVTFGDEGAYFWSPPGGKSA"
     gene            1131..2654
                     /locus_tag="VIW27_13420"
     CDS             1131..2654
                     /locus_tag="VIW27_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5740721.1"
                     /translation="MKPVRISYPWLSTLGALALVAWLLLAGSMPLLEARQSWLEDLPA
                     PLQDGDNWPLALVLLAALLAFDGWRFMRSRRRYREQLNQYQQQIEELFDSRRELGTRA
                     RTYSDHADKLKMFISERLLETIEYDEKFLHFKNIASEVRHNGVISYDKAQTALRLAES
                     NCGNDEGRQQYRDAAASLLYLWDLLDLSTTDNIALHVANQIYDCEEHFFQAMLDHDTG
                     PAPYQPTFQVSHALKRALLPITDNPEALGLTAEWPQRGEYGDDKFSIRIRQDSELLGN
                     ENHMVLLIENLLNNALFYSQQKQHRNRYARVAITLAQRSGDVELKVYNRGPRIKDEEK
                     DKIYQLGFSSRRVREHHGKGLGLYFVHEITRGFEGSIEFENIDNREGVFVISARLANG
                     DVEQHQLEVTRVKGRPMCRYLDAEPARRHEWSWESPIVSIEASQGANRKPRVISGLQK
                     SEDSTHLDDTDPMRPCWVLEVRNRRRGSRLVFTPLDVRGVEFTLRLPSARSRLDNEE"
BASE COUNT          569 a          837 c          867 g          494 t
ORIGIN      
        1 gtgttcggcc tgcaaccgcc gggcaaaaaa aaaccccgcc gaagcggggt gcaagtgagg
       61 atggtgcggg gcggtccgga cgggttgccc cggagtgaat cagtctggtt tgccaggatt
      121 cgaggtcgaa atccgttttg gcgtcctgtg ttaatcggtc atggcgagaa tttaggacgg
      181 gggtgtgaaa aatcggtgaa aaaaccccga aagcgactag aattttcgca aactactgtt
      241 ttatttagaa ttatccggtt gttaccggcg cgttcggggg gcgggcgctg gccggcgacg
      301 gctgtcgatg actaacaatc aagggatcaa aggccgatgg catcgatttt ctgggttgaa
      361 gatcaatcgc actggatcaa gaagttttcc ccggtgctgg aaagcgagga tttcgatggc
      421 aagccgaata cgctgacgat tctcaacttt gccgaggccg cgcgccaaaa gatagcgctt
      481 accgatgtcg attcgccacc ggacgtggcg ctgctcgatg cgcgcatgaa cggcaacgac
      541 caggcgggtt tctcggtttc caacgcgctg cgcaagaagt ggccacggct gccgatcatt
      601 tatctatccg agcacagcgg taccgagatc gagcgcgacg cgctgcagca gttttcggcc
      661 accgacttca tcgccaagca ccagcacaac gtcgagcagg tactgtgctg gcggatcaag
      721 gccgtgctca ggcaatcgtc gatcgacagt gacctgtcga gcaccatcga cgtgatcacc
      781 agcggcgaat tgaccatcga cctgaatacc tggaacgtct actggaaagg gcagcgcctg
      841 atgaacccga gcaatacgcg ccggccgctg gcaccgacac cgcgcaagtt gctgcgcgag
      901 ctggtcgagg tatcgccgcg gccgctgacc gccgggcagg tcgccgagcg tctgaatctc
      961 gatcccggca agttttccta tggcagctat cgccagcaca tcaagaccct gcggcgggct
     1021 ttcgatgcgg tcgagagtgg attcagcgaa acctgcaacc gcggcgaggg tatcgtcacc
     1081 tttggtgacg agggtgcata cttctggagt ccccccggcg gcaaatccgc atgaagccgg
     1141 tacgcatttc ctacccctgg ctcagcaccc tgggggcgct ggcgctggtc gcctggctgc
     1201 tgctggctgg ctcgatgccg ttgctcgaag ctcgccaaag ctggctcgaa gacttgccgg
     1261 cgccgctgca ggacggtgac aactggccgc tggcgctggt tttgctggcg gcgctgctcg
     1321 cttttgacgg ctggcgtttt atgcgcagcc ggcggcgcta ccgcgagcag ttaaaccagt
     1381 accagcagca gatcgaggaa ttgttcgatt cgcggcgcga gctcggtacc cgcgcgcgca
     1441 cctactccga ccacgccgac aagctgaaga tgttcatcag cgaacgcttg ctggaaacca
     1501 tcgagtacga cgagaaattt ctgcatttca agaacatcgc ttccgaggtt cgccacaacg
     1561 gcgtcatcag ctacgacaag gcgcaaaccg cgctcaggct ggcggagtcg aattgcggga
     1621 acgacgaggg ccgccagcag taccgcgatg cggccgccag cctgctctac ctgtgggacc
     1681 tgctcgacct gtcgaccacc gacaacatcg cgctgcacgt cgccaaccag atctacgatt
     1741 gcgaggaaca cttcttccag gcaatgctcg accacgacac cgggcccgcg ccctaccagc
     1801 cgaccttcca ggtcagccac gcgctgaagc gtgccttgtt gccgatcacc gacaatcccg
     1861 aggcactcgg cctgaccgcg gaatggccgc agcgcggcga gtacggcgac gacaagttca
     1921 gtattcgaat tcgccaggac agcgagttgc tgggcaacga aaaccacatg gtgctgctga
     1981 tcgagaacct gctcaataat gcgctgttct attcgcagca aaaacagcac cgcaaccgtt
     2041 acgcgcgggt ggcgattacg ctcgcccagc gcagcggcga cgtcgagctc aaggtctaca
     2101 accgcggccc gcgcatcaag gacgaggaga aggacaagat ctaccagctc ggattttcgt
     2161 cgcggcgcgt tcgcgagcac cacggcaagg gcctcggcct ttatttcgtg cacgagatta
     2221 cccggggttt cgagggctcg atcgagttcg aaaacatcga caaccgcgaa ggcgtattcg
     2281 tgatcagtgc caggcttgcc aacggcgatg tcgagcagca ccagctggaa gtcacccgcg
     2341 tcaagggccg gccgatgtgc cgctacctcg atgccgagcc ggccaggcgc cacgagtggt
     2401 cctgggaatc gccgatcgtc agcatcgagg ccagccaggg agcgaatcgc aagccgcggg
     2461 tgatctccgg gttgcagaaa agcgaggaca gcactcatct cgacgacacc gatccgatgc
     2521 gcccgtgctg ggtgctcgag gtgcgcaatc gacggcgcgg ttcgcggctg gtattcacgc
     2581 cgctcgacgt gcgcggcgtc gagttcacat tgcgcctgcc gagcgccaga tcacggctgg
     2641 ataacgagga gtaaggcgaa tttcgcggcg gtcggcggcg ccgtgagccg ccgctggccg
     2701 tgccagccga tcgattcccg cctgcgggca agcgggtgat tgcacaagtc gctggcatga
     2761 tcggccc
//
LOCUS       DATCZV010000596         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_154103, whole genome shotgun sequence.
ACCESSION   DATCZV010000596 DATCZV010000000
VERSION     DATCZV010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154103"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VIW27_13425"
     CDS             complement(<1..213)
                     /locus_tag="VIW27_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY5740722.1"
                     /translation="MRELLFEPMTTTMQTLFKDRVKTNILVFEPRIDVLSLELDTSVL
                     REGKLLLRLEYAIRATNSRFNLVYPFY"
     gene            complement(220..405)
                     /locus_tag="VIW27_13430"
     CDS             complement(220..405)
                     /locus_tag="VIW27_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740723.1"
                     /translation="MSNEEKEPGYDSFLGSGWSFPPRFNPHTRQIEMTADAEDIEASL
                     KILLGTTAGERILHPAY"
     gene            complement(444..722)
                     /locus_tag="VIW27_13435"
     CDS             complement(444..722)
                     /locus_tag="VIW27_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY5740724.1"
                     /translation="MPAAAKVGDTTTHGGTIVGPGAPTVLIGGMPAAVVGDTHICSLP
                     PNAHQPTASPFPSGSATVMIGGVPALRTTDSCICGAMSAVGEPTVLIG"
     gene            complement(733..2376)
                     /gene="vgrG"
                     /locus_tag="VIW27_13440"
     CDS             complement(733..2376)
                     /gene="vgrG"
                     /locus_tag="VIW27_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEY5740725.1"
                     /translation="MSINSTINRIATARLVYQDGSAARGEFRLSDSARFVPGAEVEIQ
                     AGASDQPVTLFSGVVTRHSLKIRERSAPQLIVECRHAASRMTINANNGYFLEQSDSDV
                     IGALFEAAGISADVAATPVTHEQLVQYHASDWDFCLMRAEANGLVIVTRPDGLSVKAP
                     DLSAEPVVALQFGATILEADLQLEARYQFSAVKTRFWDPASQETVELEAADPGNESPG
                     NLGSDDLAGVVGLDAYSLMHTGLAESEAQAWADGRWARSQLNRVGGCIKCEGIGSIAA
                     GDRVSLAGVGDRFDGDAYVSGVRHDFDLVQGWKTWLQFGGIEGLSEAGKSPSATPAMG
                     LLPAVNGLQIGIVVANEDPEGEFRVRVRMPLVDSEDEGTWARVASLDAGADRGWFIRP
                     EIGDEVVLGFFEDDPRQPVIIGMLNSSARAAPLDGSDDNHEKVYQTRSQMKMLFDDDK
                     VSMQFSTPAENRILLSEEDEAITLSDQHGNSIVLNSDGITLESASDLNIKSTGDLTIE
                     AGAALELKAGTDLKGEGASGVELSSSASLKISGSLVEIN"
BASE COUNT          439 a          800 c          763 g          514 t
ORIGIN      
        1 gtaaaacggg tagaccaggt tgaagcggga attggtcgcg cgaatcgcgt actcgaggcg
       61 cagcagcaat ttaccctccc tcagcaccga ggtatccagc tccagcgaga ggacatcgat
      121 acgcggttcg aaaaccagta tgttggtctt gacccgatcc ttgaacaggg tctgcatcgt
      181 cgtcgtcatc ggctcgaaca gcagttcccg caggttgagt caataggccg gatgcaggat
      241 gcgttctccg gcggtggtgc ccagcaggat tttcaggctc gcctcgatgt cttccgcgtc
      301 agcggtcatt tcgatctgtc gcgtgtgcgg attgaaacgc ggcgggaaac tccagccgct
      361 gccgagaaac gagtcgtagc ctggttcttt ttcttcgtta ctcatactcg cctggtgcct
      421 cttgcaatct cgttaattcc agctcacccg atcagcacgg tcggttcacc gaccgccgac
      481 atcgccccgc aaatacagct gtcggtcgtg cgcagcgccg gcacgccgcc gatcattacc
      541 gtggcgctgc cgctcggaaa cgggcttgcg gtcggttgat gcgcattggg cggcagcgaa
      601 cagatatgag tgtcgccgac gaccgccgcg ggcatgccgc caatcaggac cgtcggcgcg
      661 cccggcccga cgatggtgcc accgtgcgtc gtggtatcgc ctacttttgc tgccgctggc
      721 atccgctgtt cctcaattga tctcgaccag gctgccgctg atcttcagcg acgcactgct
      781 cgacagttcg accccggacg caccctcgcc cttgagatcg gtgccggcct tcaattccag
      841 cgcggcaccc gcctcgatgg tcagatcgcc agtactcttg atgttcagat ccgatgcact
      901 ctccagggtt atgccgtcac tgttcaacac gatgctgtta ccgtgctggt cgctgagggt
      961 gatcgcctcg tcttcctcac ttaacaggat tcgattttcc gccggggtcg aaaactgcat
     1021 ggagaccttg tcgtcgtcga acagcatttt catttgcgag cgggtctggt agaccttttc
     1081 gtggttgtcg tcgctgccgt cgagcggcgc ggccctggcg ctgctgttga gcatgccgat
     1141 aatcaccggt tgacgcgggt catcctcgaa aaagccgagc actacctcgt cgccgatctc
     1201 gggacgaatg aaccagccgc gatcggcacc ggcgtcgaga ctcgcgaccc gggcccaggt
     1261 gccctcgtcc tcgctgtcga ccagcggcat cctgacgcgc acccgaaatt cgccttccgg
     1321 atcctcgttg gcgaccacga tgccgatctg caaaccattc actgccggca gcaatcccat
     1381 cgccggtgtc gccgatggcg acttgccggc ttcgctcaaa ccctcgatac cgccgaactg
     1441 cagccaggtc ttccagccct gtaccaggtc gaaatcgtgg cgtaccccgg aaacataggc
     1501 atcgccgtcg aatcgatcgc cgacgccagc gaggctgacc cggtcgccgg cggcgatgct
     1561 gccgatgccc tcgcatttga tgcagccgcc gacacgattc agctgcgacc tggcccatcg
     1621 cccgtcagcc catgcctgag cctcgctctc ggcgagcccg gtatgcatca gcgagtaagc
     1681 gtcgagcccg accacgccgg cgagatcgtc actgccgaga ttaccggggc tttcgttgcc
     1741 gggatcggcg gcttcgagtt cgacggtttc ctggctcgcc gggtcccaga acctggtttt
     1801 caccgcactg aactggtagc gtgcctcgag ctgcaaatcg gcttcgagta tggtggcgcc
     1861 gaattgcagg gcgaccaccg gctcggccga cagatcgggg gcctttaccg acaacccgtc
     1921 gggacgggtg acgatgacca gcccattggc ctcggcgcgc atcaggcaaa aatcccagtc
     1981 gctggcgtgg tactggacca gttgctcgtg ggtgaccggg gttgcggcca cgtcggcact
     2041 aataccggcg gcctcgaaca gagcaccgat cacatcgctg tcgctctgtt cgagaaaata
     2101 gccattgttg gcgttaatgg tcatcctgct ggccgcgtga cggcattcga cgatcagttg
     2161 tggtgcactg cgctcgcgga tcttcagcga gtgtcgggtg acgacgccgc taaacagcgt
     2221 gaccggttgg tcgctggcgc cggcctgaat ctcgacctcg gcacccggca cgaacctggc
     2281 gctatcggaa agccgaaact cgccgcgtgc ggcggatccg tcctggtaga ccaggcgtgc
     2341 cgtggcgatg cggttgatcg tcgaattgat cgacaaggcg agtaaatgtt cgccccggcc
     2401 cagcgcctcg ccgtcgacct tgacgatgaa ttcgcgctgg gctgcggata tcggtagtgt
     2461 gcgcgcgctg gtaatggcta tttctccagg ggtggaaaat taagtggggt gccggg
//
LOCUS       DATCZV010000597         2619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_471538, whole genome shotgun sequence.
ACCESSION   DATCZV010000597 DATCZV010000000
VERSION     DATCZV010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471538"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..>2619
                     /gene="gltB"
                     /locus_tag="VIW27_13445"
     CDS             37..>2619
                     /gene="gltB"
                     /locus_tag="VIW27_13445"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY5740726.1"
                     /translation="MLAKAEQEAVGSMGDDTPMPVLSQQIRSPFDYFRQQFAQVTNPP
                     IDSLRERNVMSLQTNIGRETSLFDIGPQHANHVIMNSPVLSQRKLRQLLGHSMFADSH
                     AFIDLNLPKDLPLPDALDRICAQADQAVADDKLILLLSDRYLKPDLIPVHALLATGAV
                     HHHLVAKGQRCAVNIIVETGVARDPHHFACLIGYGATAVYPYLAYQCLQNLARKGSVD
                     ARQQLDRSYRRGIRKGLFKIMSKMGISSVSSYRGAQLFEIVGLHDEVVDRCFRGTVSR
                     IQGANFADLHEDQERLATYAWEPRQTVRQGGLLKYVHGGEYHCFNPDVIATLQAAVRS
                     GDFDRYRDYARLVNDRPAATLRDLFGLRPAGPPVPLDEVEPIADILPRFESAGMSLGA
                     LSPEAHEALAIAMNRIGGRSNSGEGGEDPARYDSERRSKIKQVASGRFGVTAEYLVNA
                     EVLQIKIAQGAKPGEGGQLPGHKVNEMIARLRYAKPGVGLISPPPHHDIYSIEDLAEL
                     IHDLKNANREARISVKLVSEVGVGTIAAGVSKAHADVVLISGYDGGTGASPLSSIKHA
                     GIPWELGLAETHQTLVLNNLRSRIVVETDGQLKTGRDVVIATLLGAEEFGFATAPLVV
                     LGCILMRVCHLNTCPVGVATQDPRLRAKFTGSADHVVNFMRFIAQEVRELMAQLGFRT
                     LNEMVGRSDRLEVNQAIAHWKARGLDFSNILHQPAVGPEIGRYCQIAQDHGLARSLDV
                     TQLLDLCRPAIDRGEPVRARLPIRNINRVVGTILGYELTKRYGPAGLPDDTIQLKFDG
                     SAGQSFGAFVPHGITLTLEGDANDYVGKGLSGGKLIIYPPKGSTFAAEENIIVGNVAF
                     YGATQ"
BASE COUNT          501 a          876 c          801 g          441 t
ORIGIN      
        1 aatctctccc gggaggaatt gaacgaagtc atcaccgtgc ttgcaaaagc cgagcaggaa
       61 gctgtcggat ccatgggcga tgacacgcca atgccagtgc tgtcacagca gatcaggtcg
      121 ccgttcgatt atttccggca gcaatttgcc caggtaacga atccgcccat cgactcgctt
      181 cgcgaacgca acgtgatgtc cctgcaaacg aacatcggcc gcgaaaccag cctgttcgac
      241 atcgggccgc agcatgccaa ccacgtcatc atgaattcgc cggtcctgtc gcagcgcaag
      301 ttgcgacagc tcctcggcca cagcatgttc gccgacagcc atgcgttcat cgacctcaac
      361 ctgccgaagg acctgcccct gcccgacgcc ctcgaccgga tttgtgcaca ggccgaccag
      421 gcagttgcgg acgacaagct gattctgctg ctgagcgatc gctacctgaa gccggacctc
      481 atcccggtgc atgccctgct ggcgaccggc gcagtacatc atcacctggt agccaaaggc
      541 cagcgttgcg cagtcaacat catcgtggaa accggtgtcg ccagggatcc gcatcatttc
      601 gcctgcctca ttggttacgg cgcgaccgcg gtttacccct acctcgcgta ccagtgcctg
      661 cagaacctcg cgcgaaaagg cagcgttgat gcgcggcagc agctggatcg cagctatcgt
      721 cgtggcatac gcaagggtct tttcaagatc atgtcgaaga tgggcatctc gtccgtgtcg
      781 agctatcgcg gtgcacagct ttttgaaatc gtcggcttgc acgacgaagt cgtcgatcgc
      841 tgtttccggg gaacggtgag ccgtatccag ggcgcaaatt ttgccgacct gcacgaggac
      901 caggagaggc ttgcgacata cgcctgggaa ccacggcaga ccgtcaggca gggcggattg
      961 ctcaaatacg tgcacggtgg cgaatatcac tgcttcaatc ccgacgtcat cgcgaccctg
     1021 caggctgcgg tacgatccgg ggatttcgac cgctatcgcg attacgcgcg gctggtcaac
     1081 gacaggcccg cggcgacgct gcgcgatctg ttcgggctca ggcccgcggg acccccggta
     1141 ccgctcgacg aggtcgagcc gatcgcggac atactgccgc gattcgagtc cgccggcatg
     1201 tcgctcggcg cgctgtcacc cgaagcgcac gaggcgctgg cgattgcgat gaaccgtatc
     1261 ggcggccgct cgaattcggg cgagggcggc gaggacccgg cgcgctatga ctcggagcgc
     1321 cgatccaaga taaaacaagt ggcatcgggt cgattcggcg ttacggcgga atacctcgtc
     1381 aatgcggaag tcctgcagat caaaatcgct cagggcgcaa aacccggcga aggcggtcag
     1441 ctaccgggcc acaaggtcaa cgagatgatc gcgcggctgc gctacgcgaa acccggcgtc
     1501 gggctgattt ccccgccgcc gcatcacgac atctattcga tcgaggacct ggccgagctg
     1561 atccacgacc tgaaaaacgc caaccgcgaa gcccgcatca gcgtgaaact cgtctccgag
     1621 gtcggcgtcg gcacgatcgc cgccggcgtt tccaaggccc acgccgacgt ggtcctgatc
     1681 agcgggtacg acggcggcac gggcgcctcg ccgctctcgt cgatcaagca tgccggcatc
     1741 ccctgggaac tcggcctggc cgagacccac cagacgctcg tgctcaacaa cctgcgcagc
     1801 cgcatcgtcg tcgagaccga cggccagctc aagaccggtc gcgacgtggt gatcgccacc
     1861 ctgctcggcg ccgaggagtt cgggttcgcc accgcgccgc tggtcgtgct cggttgcatc
     1921 ctgatgcggg tttgtcacct caacacctgc ccggtcggcg tggccaccca ggacccgcgc
     1981 ctgcgggcga agttcaccgg cagcgcggat cacgtggtga atttcatgcg gttcatcgcc
     2041 caggaagtcc gcgaactgat ggcccagctc gggttccgca ccctcaacga gatggtcggg
     2101 cgcagcgatc gtctcgaggt caaccaggcg atcgcccact ggaaagcccg cgggctggat
     2161 ttctcgaaca ttctccacca gccggccgtc ggcccggaaa tcgggcggta ctgccagatc
     2221 gcccaggacc acgggctcgc gcgttcgctg gacgtgacgc agctgctcga tctctgccgc
     2281 ccggccatcg accgcggcga gccggtccgc gcgcggctgc ccatccgcaa catcaaccgc
     2341 gtggtcggca cgatcctcgg ttacgagctg accaagcgct acggcccggc ggggttgccg
     2401 gacgacacga tccagctcaa gttcgacggc tcggccggcc agagtttcgg cgcgttcgtg
     2461 ccgcacggga tcacgctcac gctcgagggc gacgccaacg actacgtcgg caagggattg
     2521 tccggcggca aactgatcat ctacccgccc aagggctcga ccttcgccgc cgaggaaaac
     2581 atcattgtcg gcaacgtcgc gttctacggg gcgacccag
//
LOCUS       DATCZV010000598         6467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_418671, whole genome shotgun sequence.
ACCESSION   DATCZV010000598 DATCZV010000000
VERSION     DATCZV010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418671"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VIW27_13450"
     CDS             <1..1291
                     /locus_tag="VIW27_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740727.1"
                     /translation="VILASTWLYWLLGRGKTRTPKLLNLKQIKRLEPLVNTYISTAGV
                     EYSDAFLHDNDARFVFNFVRSAMDKGCVAANYVASEGATRDSNGQWITQVKDQVSDDR
                     FSIRARVLINAAGAYVDQHNGLTGIETEHRHLLSKGIHLLVPRITETHRVLAFFADDE
                     RLFFAIPMANRTCIGTTDTPVEDPVTQVTDDDRDFVLSNINARLDLSPPLTRKDIIAE
                     RCGVRPLAIRKTIDASADLRQVSRKHVIEMDADNRHISIFGGKLTDCINVGDEISDCV
                     SRLGLKVSRPAGKWYGEPGRREQEAYFERARALGLDQLVAPDTGEALSERLWRRYGAN
                     ASTMLEAIEQNPGLREPMIDNTGIRRCEIAYLAKHEMIVKLEDYLRRRSKIELLVPQA
                     TLRQSAGLFEACEKLFGDDGKKRFDEYFASSEKDDAT"
     gene            1322..2812
                     /gene="glpK"
                     /locus_tag="VIW27_13455"
     CDS             1322..2812
                     /gene="glpK"
                     /locus_tag="VIW27_13455"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY5740728.1"
                     /translation="MSQYILSIDQGTTSSRAMLFDETGHAAFTAQQEFTQHFPRDGWV
                     EHNAEEIWVTTLAVVREAIAKARADRRDIAAIGITNQRETTVVWDRSSGEPVYHAIVW
                     QDRRTADYCDSLRSQGHELMVTEKTGLLLDPYFSGTKVNWILDNVSGARARADNGELA
                     FGTIDCFLIWRLTGGKVHATDATNASRTLMFNIHQQRWDSELLDMLTIPDSLLPEVRD
                     CAADFGNTSKNLLDAEIPIAGVAGDQQAALIGQACFEPGMIKSTYGTGCFMILNTGEQ
                     ALASGNRLLTTVGYRLNGKTTYALEGSIFVAGAAVQWLRDGIGIIDSAHETEALAASL
                     DSNMGVYLVPAFTGLGAPHWDPDARGAIFGITRDTGVAELVRATLESVCYQTFDLLEA
                     KRRDGLAPTRLRVDGGMVQNNWLCQFLADMLDIIVERPVETETTALGAAYLAGLQVGL
                     FTSLEDISTRWRAEREFDPAMDSSQRNLLLADWHEAVIKVKTGAQA"
     gene            complement(2814..4001)
                     /locus_tag="VIW27_13460"
     CDS             complement(2814..4001)
                     /locus_tag="VIW27_13460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740729.1"
                     /translation="MFDRRPAALLVVSGGAGESFARLNALEGIARSLLMGVIPLLALE
                     ALGSKELVTHAYLFASILTLAITLNFATLERLLQRRWVVTLGAGCTLVAMLILLFGDG
                     MIMALGIGLQQASASLFSVCLSLYIMDYIGKKELIYTETRRMLYAGVVWMVGPILGLW
                     LWEGIAGWAPIVLSLFGAGAMLSYFWYLRLGHGQGIQRAKSHPASVFKIIPRYFGQRA
                     LRIAYWITLSRAIFWVTLFVYGPIYVVEAGLPNWIAGGLLSLASALLLVSPLIRRLAS
                     RIGTRRVILFALMLTGLSMAMLYVLGEARPIGLLFWITGALGGVTLDVLGNIPFMRMV
                     KPRERTEMTMIFSTWREGSQLLTPLLVSTMLLFAPFETFYLLLALLLSGAGIMATFLP
                     RRL"
     gene            complement(4231..5235)
                     /locus_tag="VIW27_13465"
     CDS             complement(4231..5235)
                     /locus_tag="VIW27_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017003359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5740730.1"
                     /translation="MKALVYTAPLELEYRDEPDPMPEAGEALIRVEASGICGSDMHAY
                     YGHDERRPAPLILGHEVSGEVVSGSRKGQRVVVNPLVTCGQCDDCLSGRANLCRSRQI
                     ISMQPRQGAFSELLRMPERNLVSLPDDMSYTHAALTEPVATAMHAVLEGERLGRRPMA
                     ASRVLVLGGGAIGIAVALILYSHGCRNIRLGETTRLRRQTVDRTGSCEVYDPLNDPAP
                     EADSWDIVIDAVGGAATREAASKAIKPGGVIVHIGLMDNNGGLDVRKLTLQEVTFIGC
                     YTYTMVDVRATVNALYSGALGSLDWIEQRPLSEGAAAFADLAAGNTPAAKIVLIPDRN
                     "
     gene            complement(5245..6021)
                     /locus_tag="VIW27_13470"
     CDS             complement(5245..6021)
                     /locus_tag="VIW27_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017007651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5740731.1"
                     /translation="MLDNIRQLFDLSGRVALCTGGSSGIGRRMALALSQAGAEVVLVG
                     RNEAALAEAMAQIKTEGGGRGVTIAADLLDRSTLGDVVAEAAEAFGAPDILINAAGIN
                     LREPYDDISEASWQQTLDINLSTPFFLARHCIPAMKSNGRGNIINVASLQSFRAFSNS
                     MAYGASKGGIAQLTRAMAEAWSPFGIVSNAVAPGFFPTELTRKVYENPELLAHNASMT
                     AIGRNGELSDLDGVTVFLASAAANYITGQIIAVDGGFTAK"
     gene            complement(6057..>6467)
                     /locus_tag="VIW27_13475"
     CDS             complement(6057..>6467)
                     /locus_tag="VIW27_13475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5740732.1"
                     /translation="VILCDTREEVAQISDQYAGEHLEVQAKDLDWWLGRLKNYGSLFV
                     GEEATVSFGDKCSGTNHILPTKGAGRYTGGLSAAKFIKVVTYQRQTREATREVGAACA
                     RIARAEGMEGHARAGDIRLAKYFPDETFDLGELR"
BASE COUNT         1427 a         1966 c         1876 g         1198 t
ORIGIN      
        1 ggttatcctt gccagcacct ggctttactg gctgctcggc cgcggcaaaa cgcgaacacc
       61 gaaactgctt aacctgaaac agatcaaacg gctcgagccg ctggtgaaca cctatatcag
      121 cacggctggc gtggagtact ccgacgcctt tttgcacgac aacgacgccc gcttcgtctt
      181 caacttcgtg cgcagcgcaa tggacaaggg ctgcgttgcg gcaaactacg ttgcctcgga
      241 gggcgcgacg cgggattcga acggacagtg gatcacccag gtaaaagatc aggtcagcga
      301 cgatcgtttt agtatccgcg cgcgggtatt gatcaacgcg gcgggcgcct acgtcgacca
      361 gcataacggg ctcaccggca tcgaaaccga gcaccggcac ctgctgtcga agggcataca
      421 cctgctggtg ccgcgcatca cggaaacgca tcgtgtgctg gcgttttttg ccgacgacga
      481 gcgcctgttc ttcgccattc cgatggcgaa ccgaacctgt atcggcacca ccgacacccc
      541 ggtcgaggat cccgtaacgc aggtaaccga tgatgaccgc gatttcgtgt tatcgaatat
      601 caacgcgcgc ctcgacctgt ccccaccgct aacccggaaa gatatcatcg ccgagcgctg
      661 tggcgtgcgc ccgctggcaa tccgcaaaac catcgatgcc tcggccgacc tgcggcaagt
      721 atcgcgcaag cacgtcatcg aaatggacgc cgataacagg cacatcagca tattcggcgg
      781 caagctgaca gactgtatca acgttggtga cgagatcagc gattgcgttt ctcggctcgg
      841 cctgaaggtt tcgcggccgg caggtaagtg gtatggagaa ccggggcggc gcgagcagga
      901 ggcgtatttc gagcgcgcgc gagcacttgg tctcgaccaa ctggtagccc cggacaccgg
      961 cgaggctctg agcgagcgtc tgtggcgacg atacggggca aatgccagta caatgctcga
     1021 agcgattgaa cagaatcccg gtttgcgcga accgatgata gacaataccg gcatccgtcg
     1081 ctgcgagatc gcttacctcg caaaacacga aatgattgta aagctcgaag attatctcag
     1141 gcggcgttcc aagatcgagc tgctggtacc gcaagccaca ctgcgtcaat cggccgggtt
     1201 gttcgaagcc tgtgaaaaac tgttcggtga cgatggcaag aagcgcttcg atgaatactt
     1261 cgcttcgtcc gaaaaagacg atgcgacctg aagttgtaac tggatgattt ggaggggtta
     1321 gatgagccag tatattctca gcatcgacca gggcacgacc agctcacgcg ccatgctgtt
     1381 cgacgaaacc ggccatgccg cgtttaccgc gcaacaggag tttacgcagc attttccaag
     1441 ggatggctgg gtcgaacaca acgccgagga gatctgggtc acgaccctgg ccgtggtgcg
     1501 cgaggcgata gcaaaagcgc gggccgaccg acgcgacatt gccgccatcg gcatcaccaa
     1561 tcagcgcgag accaccgtgg tctgggaccg gtccagcggt gaaccggtgt atcacgccat
     1621 cgtctggcag gaccgccgca ccgccgacta ctgcgactcg ctgcgcagcc agggacatga
     1681 attgatggtg accgaaaaga ccggcctact gctcgatccc tatttttcgg gcaccaaggt
     1741 caactggatt ctcgataacg tcagtggggc gcgagcccgc gccgacaacg gcgaactcgc
     1801 cttcggcacc atcgactgtt tcctgatctg gcgcctgacc ggggggaagg tacacgccac
     1861 cgatgccacc aacgcatcgc gcacgctgat gttcaacatt caccagcagc ggtgggatag
     1921 cgagctgctc gacatgctga caatacccga ctcgctgtta cccgaagtac gcgactgtgc
     1981 cgccgatttc gggaatacca gcaagaacct gctggatgcc gaaattccca ttgccggcgt
     2041 cgccggcgac caacaggcgg cgctgatagg ccaggcctgt ttcgaacccg gtatgatcaa
     2101 gagcacttac ggtaccggct gtttcatgat tttgaacacc ggcgaacagg ctctggcctc
     2161 cggtaaccgg ttactgacca cggtcggtta ccgactgaac ggcaaaacga cttacgcgct
     2221 cgagggttcg atttttgtcg ccggcgcggc ggtgcaatgg ttgcgcgacg gtatcggcat
     2281 catcgattcg gcgcatgaaa ccgaggcgct ggccgcatcg ctggattcga acatgggcgt
     2341 ttacctggta ccggcgttca ccggcctcgg cgcgccgcac tgggaccccg acgcgcgcgg
     2401 cgccatcttc ggcatcaccc gcgataccgg ggtcgcggag ctggtgcgtg ccacgctgga
     2461 atcggtgtgc tatcaaacct tcgatctgct cgaggccaag cgtcgagacg gcctcgcacc
     2521 aacgcgcctg cgggtcgacg gcggtatggt gcaaaataac tggctgtgcc agtttctcgc
     2581 cgacatgctc gacattatcg tcgaaaggcc ggtggaaacc gaaaccaccg cgctcggcgc
     2641 cgcttatctc gcgggattac aggtcggcct gtttacttcg ctagaagata tctcgacccg
     2701 ctggcgggcc gagcgtgaat tcgatccggc catggattcc agccagcgta acctgttact
     2761 ggcggactgg cacgaagcgg tgatcaaggt aaaaaccggt gcccaggcat agattacagc
     2821 cggcgcggca ggaaggtagc catgattccg gcgccgctaa gcaatagcgc gagcagcagg
     2881 taaaaagttt cgaacggggc gaacagcagc atcgtcgaca ccagcagcgg ggtcaataac
     2941 tgcgaaccct cgcgccaggt cgaaaatatc atcgtcatct cggtgcgctc gcgcggcttg
     3001 accatgcgca tgaaggggat gtttccgagc acgtcgagtg taaccccgcc cagcgccccg
     3061 gtaatccaga acagcaagcc aatcggcctg gcctcgccga gcacatatag catcgccatt
     3121 gacaggccag ttaacatcag ggcaaacagg atgacgcgcc gggtaccaat gcggctagcc
     3181 aggcggcgta tcagggggct aaccagtaac agggcgcttg ccagcgacag caggcctccc
     3241 gcaatccagt taggcagacc cgcctcgacc acataaatag ggccgtagac aaacagggtc
     3301 acccagaata tcgcgcgcga cagggttatc cagtaggcga ttcgcagcgc gcgttgtcca
     3361 aagtagcgcg ggatgatctt gaaaacgctg gccggatgcg atttcgcccg ttgaattccc
     3421 tgtccatgcc cgagacgcag gtaccagaag taggacaaca tcgcgcccgc accaaacaga
     3481 ctgagcacga ttggcgccca gcctgcaata ccttcccata accacagacc cagaattgga
     3541 ccaaccatcc agacgacgcc cgcgtaaagc atgcgcctgg tttcggtgta aatcagttcc
     3601 tttttgccga tgtagtccat gatgtatagc gacaggcaaa ccgagaacag cgaggcggac
     3661 gcctgttgca aaccgatacc tagggccatg atcatgccgt caccgaacaa caggatcagc
     3721 atcgcgacca gggtgcagcc ggcgcccagg gtcaccaccc agcgccgctg cagcaggcgt
     3781 tccagagtgg caaaattgag cgtaatagcg agcgtcagga tggaggcaaa caggtaggcg
     3841 tgggtcacta attccttgga gcccagggcc tccagcgcca acagcggaat tacccccatc
     3901 aatagcgagc gtgcaatgcc ctcgagcgca ttgagccggg cgaaggattc tcctgccccg
     3961 ccggaaacga ccagtaacgc cgcggggcgc cgatcaaaca aaacgaatcc ttagggtttc
     4021 ttaacttgct ggaatgctag gtgaaacagg agtcaaagga taaccaatta tatcgatcgc
     4081 gcggatcgaa atagcaaatg acgctgacct gaaaatgacg ctgacctgaa aatgacgctg
     4141 acctaacgat cgaatcccgg cataaccctg ggatctgcgc aatgctgcag gagctcgaag
     4201 agcgtaacgc cgaactcgat atcccgtgtg tcagtttcgg tccggtatca atacgatctt
     4261 ggccgctggc gtattacccg ctgccagatc ggcaaacgcc gctgcacctt cactgagcgg
     4321 acgctgctct atccagtcca gtgaacccag ggcacccgag tacaacgcgt ttaccgtggc
     4381 cctgacatcc accatggtat aggtataaca accgatgaag gtgacttcct gcaaggttaa
     4441 tttacgcacg tcgagcccac cgttgttatc catcaaaccg atatgaacta tgaccccacc
     4501 gggtttgatt gccttcgatg ccgcttcgcg cgtggcggcg ccgcccaccg catcgatgac
     4561 gatgtcccag ctatcggcct cgggcgccgg atcgttgagc ggatcataga cttcgcatga
     4621 gccagttcgg tcgacggtct ggcggcggag tcgagtggtt tcgccaagac gaatattacg
     4681 gcagccgtgg ctgtagagga tcaacgccac ggcaataccg attgcaccgc caccgaggac
     4741 cagcactcga gaagcagcca tgggccgacg acccagcctt tcgccttcca gtacagcatg
     4801 catcgccgtg gcgaccggtt cggtcagggc agcgtgggta tagctcatgt catcgggaag
     4861 gcttaccagg ttacgttcgg gcattctgag cagctcggaa aacgcgccct gccggggttg
     4921 catactgata atctggcgcg agcgacacag gttggcccga ccgctaagac agtcgtcgca
     4981 ctgaccacag gtaacgagcg ggttaaccac tacccgctga cccttgcggc tgccgctgac
     5041 gacttctcca ctcacctcgt gaccgaggat gagcggcgcc ggacgccgtt cgtcatggcc
     5101 gtaataggcg tgcatatcgg agccgcagat gccgctcgct tcgacgcgga tgagcgcttc
     5161 gcccgcttcg ggcatcgggt cgggttcgtc gcggtactcg agctccaagg gtgcggtata
     5221 aaccagtgcc ttcatatcta tcctctactt tgcggtaaat ccaccgtcga cagcaataat
     5281 ctgtccggtg atgtagttgg cagccgccga cgccaggaaa acggtgacgc catccagatc
     5341 ggatagctca ccatttcgac cgatggccgt catgctcgcg ttgtgcgcca gcaactcggg
     5401 attttcgtag accttgcggg tcagctcggt cgggaaaaag cccggcgcga cggcattact
     5461 gacgatgccg aacggcgacc aggcttccgc catggcacgc gtcaattgcg caattccgcc
     5521 cttggacgcg ccgtaggcca tgctattaga gaaggcgcga aaagattgca gtgacgcgac
     5581 gttaatgatg ttaccccgac cgttgctttt cattgcggga atacaatgcc gcgccagaaa
     5641 gaacggtgtc gataaattga tatccagtgt ctgctgccag ctggcctcgc tgatatcatc
     5701 gtagggctca cgcagattga taccggccgc attgatcagt atatccggcg cgccgaacgc
     5761 ctccgcagcc tcggcaacga catcgccgag ggtagaacga tcgagcagat cagccgcaat
     5821 tgtcacgccg cggccgccac cctcggtttt tatctgcgcc attgcctcgg ccagcgcggc
     5881 ctcgtttcga cccaccaaca caacctcggc accggcttgc gaaagcgcca gcgccatgcg
     5941 ccggccgatg ccggagctgc cgccggtaca cagcgcgacc cttccgctca aatcgaacag
     6001 ctgacggatg ttgtccagca tctggctact cgcttcgcct gccgaaacgc ccgtgactaa
     6061 cgcagctcgc ccaggtcgaa tgtttcatcg gggaagtact tcgccaggcg aatatcaccg
     6121 gcccgggcgt ggccttccat gccttcggcg cgcgctatgc gggcacaggc tgctcccacc
     6181 tcgcgggttg cttcccgggt ctggcgctga taggtcacca ccttgatgaa tttggccgca
     6241 ctcaggccgc cggtgtagcg tcctgcgcct ttggtcggaa gaatatgatt ggtgcccgag
     6301 cacttgtcgc cgaaagaaac cgtggcctct tcaccgacaa acagggaacc gtagttcttc
     6361 aaccgaccga gccaccaatc caggtctttg gcctggacct cgaggtgctc gcctgcatac
     6421 tgatcgctga tctgggcaac ttcctcgcgt gtatcgcaaa gaatgac
//
LOCUS       DATCZV010000599         5657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_260626, whole genome shotgun sequence.
ACCESSION   DATCZV010000599 DATCZV010000000
VERSION     DATCZV010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260626"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1260
                     /locus_tag="VIW27_13480"
     CDS             64..1260
                     /locus_tag="VIW27_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY5740733.1"
                     /translation="MTRKKHNPDDKLLEALVSSDRDLLLELMRAALEEVLESEMSEQL
                     GAAPGERSEGRLGYRSGYYQRNLVTRIGKLELRVPRDRDGRFSTKLFDRYQRSEKALV
                     SALAEMYVQGVSTRKVKKITEELCGHSFSASTISRINKGLDKTLSKFAKRRLDETYPY
                     LILDGRYEKVRIDGIIQTQAVLIAIGINWEGRRQILGVSLANRESGSSWKGFLSDLRH
                     RGLSGVEFVVSDDHAGIKSALKEIIPEATWQRCYVHFLRNALDYLPRKADDDCLQELR
                     WMYARRDLKEAQRDLAAWLERWEKRYPRLTDWVEENIGETLSFYRLPRQHHKHLKSTN
                     MLERLNEEIKRRTHIVRIFPNAASCLRLVLALCVETHESWLEDHRYLNMDFLKEQKKE
                     KLREAA"
     gene            complement(1471..2502)
                     /locus_tag="VIW27_13485"
     CDS             complement(1471..2502)
                     /locus_tag="VIW27_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY5740734.1"
                     /translation="MRTTRVIHIVGCHAEGEVGNVIVGGVAPPPGDTLWEQSRFIHDD
                     GTLRNFVLNEPRGGVYVHVNLLLPAISAEADFGWIVMEPEDTPPMSGSNSMCVATVLV
                     DTGMVEMREPYTELVLEAPGGLVRLRVECVDGKAYRVTTTNVPSFADRLDAPLEVEGL
                     GTLTVDTAYGGDSFVIVNPHDIGLEIVPDAARELVEAGRRITRAANQQLGFVHPENPQ
                     WSHISFTQFAAPLSYEDGIAVSSNAVVIQPGKLDRSPCGTGCSARLAVLAARGQMRTG
                     DRLIGRSIIDSRFDCEIVETCSVGGRPAIVPTIAGRAWITGTRQLMLDPDDPWPGGYK
                     LSDTWPTKL"
     gene            2995..3267
                     /locus_tag="VIW27_13490"
     CDS             2995..3267
                     /locus_tag="VIW27_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740735.1"
                     /translation="MPPKPRTTEAMRSRIGEARAVLPLDLSPEQVCADECRGCSIKLI
                     EYLAMELDNWEYRLEDGAIPNFRDLDRLARSCSKIHRALQKNGLVE"
     gene            complement(3329..4057)
                     /locus_tag="VIW27_13495"
     CDS             complement(3329..4057)
                     /locus_tag="VIW27_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5740736.1"
                     /translation="MRALVFVHGYLGGSAQWGAQGEAFRASHRVITPDLPGYGRNRAL
                     AAPEEIGAFADYALEHARSMGVERFDLVGHSMGGMIVQEMVARAPERVDRLVLYGTGP
                     VGLLPGRFETIAESRRRVVEEGPKATGRRIAATWFVDYERAPNYPVCADLAVLASQQA
                     ALAGLTAMERWSGEAALEKIASPTLVLWGDCDRTYTWSQQQKLWQGIAGACLAVIPGC
                     AHAAHLEKPQLFNAILADFLDSGR"
     gene            complement(4065..5597)
                     /locus_tag="VIW27_13500"
     CDS             complement(4065..5597)
                     /locus_tag="VIW27_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5740737.1"
                     /translation="MAAEEITAAAARESEDLAAERSAQVERFVKTNPEYYRSQFAKIG
                     GDSKFVWTFNSWAGFFGPIWFSARGMWNWGLAFLILETLAAVQIIRGMFGDLAAEAWE
                     RIGKIEGTLELRRQQLAAAIESNSDKVDVYRRTVESLEDAIGGIRLEAQQMEDSGLAI
                     ALVGILVLLLVKAAQSVVANTLLEKRFSDWLSDPDISTGMTPRDMALSCGFVAVIMIA
                     TVIHYSFPGAYPWLIDFPTEHGIRLGSIKGVEEFFAFCVTNGEAFFDAITFAIRSVLD
                     ALELIFVQTPWMVIASFIILLTWLSAGPRAAIFSGGFLLYMGLLGFWEKAMTTLALLG
                     TAAVLSIVIGIPLGMYCARRERFYAFIRPIMDFCQTMPAFVFMIPVIAFFGTGKPAAV
                     VTTMIFGGTPVVRLTVLGLRGVPEHVREAAVSFGASKWYLLTRVDLPLASPSIRAGIN
                     QTIMLSLAMVVVASLIGAKGLGEDVLEALQYANVGQGILAGFAILFCAMILDRIVQGD
                     RK"
BASE COUNT         1149 a         1789 c         1754 g          965 t
ORIGIN      
        1 aaaagcggtc atggtatgag gttgattgtt cacatcgacc agaacccctc gggagggcat
       61 accatgacca ggaaaaagca taatccggac gacaagttgt tggaagcact cgtttcatcc
      121 gatagagatc tgttgcttga actgatgaga gcggcacttg aggaagtgct ggaatcggag
      181 atgagcgagc agctcggcgc ggcgccggga gagcgctcgg aaggtcggct gggctatcga
      241 tccggctatt accagcgcaa cctggtgacc cggatcggga aactggaact gcgcgtgccc
      301 agggaccggg atggacgctt ttcgacgaag ctgttcgatc gctaccagcg ctcggagaaa
      361 gccctggtca gcgcgcttgc ggagatgtac gtacagggtg tatcgactcg caaggtcaag
      421 aaaatcaccg aggagctgtg cggtcacagc ttctcagcct cgacgatcag ccgcatcaac
      481 aagggcctgg acaagacgct ctccaagttc gccaaacggc ggctcgacga gacctatccc
      541 tacctgattc tggatggtcg ctatgagaaa gtacgcatcg acggcatcat ccagacccag
      601 gctgtgttga ttgcgattgg catcaactgg gaaggccggc gccagatcct gggcgtctcg
      661 ctggccaatc gggagtcggg atccagctgg aagggctttt tgagcgattt gcggcatcgc
      721 ggcctgtccg gcgtcgagtt cgtcgtttcc gacgatcatg ccggcatcaa gtcggcgctg
      781 aaggagatca ttccggaagc gacctggcag cgctgctacg tgcacttcct gcgcaacgcg
      841 ctggactacc tgccacgcaa ggccgacgac gattgtctgc aggagctgcg ctggatgtac
      901 gcgcgtcgcg acctcaagga ggcgcaacgg gacctggcgg cctggctgga gcgctgggag
      961 aagcgctatc cgcggctcac cgactgggtc gaggagaata tcggtgagac gctgagcttt
     1021 taccggttac cacgccagca tcacaagcac ctgaaaagca ccaacatgct cgagcggctg
     1081 aacgaggaga tcaaacggcg gacacatatc gtacgcatct tcccgaacgc tgcttcgtgc
     1141 ctgcgcctgg tgctggcact atgcgtcgag acccatgaga gctggctgga agaccatcgg
     1201 tatctcaata tggacttcct caaggagcag aagaaggaaa agctgcgaga ggcagcctaa
     1261 tgagtaaagc ctcatccatg aatttctttt tgcacaactt gacgtacaca accgatgccc
     1321 gctttcgcgg gcatgacgat acctgagtcg ttccgccaac ccgaagtcat tcccgcaacc
     1381 cgggggcatt cccgcgcaag cgggaatcct gacgagttcc ctggcctgcc gtggccgcag
     1441 aatcccggta tcgcgactcg cgcgcccggc tcagagtttc gtcggccagg tgtcagacag
     1501 cttgtagccg ccgggccacg gatcatcggg atcgagcatc agctgccgcg tgccggtaat
     1561 ccaggcgcgg ccagcgatag tcggtacgat ggccggcctg ccgccgacac tgcaggtctc
     1621 gacgatctcg caatcgaagc gcgaatcgat gatcgaacgg ccgatcaggc gatcgccggt
     1681 acgcatttgc cccctcgcgg cgagcactgc gaggcgcgcc gaacagccgg tgccgcacgg
     1741 cgagcggtcg agcttgcccg gctggatgac gaccgcgttg ctgctcaccg cgatgccgtc
     1801 ctcgtaagac aagggcgcgg cgaactgcgt gaacgagatg tgcgaccatt gaggattttc
     1861 cgggtgcacg aaaccgagct gctggttggc cgcacgggtg atcctgcgcc cggcctcgac
     1921 cagctcgcga gccgcgtcgg gcacgatttc cagcccgatg tcgtgtgggt tgacgatcac
     1981 gaaactgtcg ccgccgtagg cggtatccac ggtcagcgtg ccgaggccct cgacttcgag
     2041 cggcgcgtcg agacggtcgg cgaacgacgg cacattggtg gtggtcacgc ggtaggcctt
     2101 gccgtcgacg cattcgacgc gcagccgaac caggccaccc ggcgcctcga gcaccagctc
     2161 ggtatacggc tcgcgcatct ccaccatgcc ggtatcgacc agcaccgtcg ccacgcacat
     2221 cgagttagaa cccgacatcg gcggcgtgtc ctcgggttcc atgacgatcc agccgaagtc
     2281 ggcctccgcg gagatcgccg gcagcagcag gttgacgtga acgtatacac cgccgcgcgg
     2341 ttcgttgagc acgaaattgc gcagcgtgcc gtcgtcgtga atgaaacgcg actgttccca
     2401 cagcgtgtct ccgggcggcg gcgcgacgcc gccgacgatg acgttgccga cctcgccctc
     2461 ggcgtggcag ccaacgatat gaatgaccct ggtggtgcgc acgaacgcct ctccgctgaa
     2521 gccggtgaaa aagtataatc gagtcacgaa atcaagtctt gcagccatcg tcggtagctc
     2581 gttcggcctg gtgtgaaatc atcgaaaccg gcgagaatcc gggcccgccc tgtcaaatcc
     2641 ggagccgggc gaggataaac cgatgaatta gcgggaatac gccgaccgag cagtccgata
     2701 ccacgcctga tacaggtctg ctatcgaagc aaaacagtgc gaaaatcgcc acggcctgtc
     2761 tgcgatacct ggtgcgctgc gcaattattc tgcttgctca atgcaatcgg ctgtttatcg
     2821 cccgctaatg gctcgttgct catcgaatgc tgttttcagg acttttaccc ggttcgatcc
     2881 aaaatactcg agcagccgat tccttccgca gcggcaatag atttgaccgc ggtcaaaacg
     2941 gggccacctg cgggggactt ataattcggc ccgaattcac gagcggattg gcaaatgccc
     3001 cccaaacccc gtaccaccga agccatgcgc agccggattg gcgaagcacg agcggttctg
     3061 ccgctcgacc tgtcgccgga gcaggtctgc gcggacgagt gccggggttg ctcgatcaag
     3121 ctcatcgagt accttgcgat ggaactggac aactgggaat accggctcga agacggcgcc
     3181 atccccaact tcagggatct cgaccggctg gcccgcagtt gcagcaagat tcaccgcgca
     3241 ctgcaaaaga acgggctggt ggagtaagtc ccgatcgtcg acctggtact ggccgcaagg
     3301 gacacgcgct gaaccgcgct gctggcggct agcgcccgga atcgagaaaa tcggcgagga
     3361 tcgcgttgaa caattgcggc ttttcgaggt gcgccgcatg cgcgcagccc ggaattaccg
     3421 cgaggcaggc cccggcgatg ccctgccaca gcttttgctg ctggctccag gtgtaggttc
     3481 gatcgcaatc gccccacagc accagcgtcg gcgaggcgat tttttcgagc gccgcctcgc
     3541 ccgaccagcg ctccatcgcc gtgagcccgg ccagcgccgc ctgttgcgag gcgagcaccg
     3601 cgagatcggc gcataccggg tagttcggcg cgcgctcgta gtcgacgaac caggtcgccg
     3661 caattcgccg cccggtggcc ttcgggccct cctcgacgac ccggcgcctg gattcggcga
     3721 tggtctcgaa gcgcccgggc aacaggccga ccggcccggt gccgtaaagc accaggcgat
     3781 cgacgcgttc gggcgcgcgc gccaccattt cctgcacgat catgccgccc atcgagtggc
     3841 cgaccaggtc gaaacgctcg acacccatgc tgcgcgcatg ctcgagcgcg taatcggcaa
     3901 aggcgccgat ctcctcgggc gccgccagcg cgcgatttcg gccatatccg ggcagatcgg
     3961 gcgttatcac ccggtgcgag gcgcgaaacg cctccccctg cgcgccccac tgggcgctgc
     4021 cgccgaggta gccgtgaacg aaaaccagcg cccgcatcgc gcgcttactt gcggtcgccc
     4081 tgcacgatgc ggtcgaggat catcgcgcag aacaggatcg cgaaaccggc gaggatgccc
     4141 tggccgacgt tggcgtactg cagcgcctcg agcacgtcct caccgagacc cttggcgccg
     4201 atcagcgagg caaccaccac catcgccagg ctcagcatga tggtctggtt gatgccggcg
     4261 cgaatcgacg ggctcgccag cggcaggtcg acccgggtga gcaggtacca cttgctggcg
     4321 ccgaacgaaa ccgccgcctc gcgcacgtgc tcgggtacgc cgcgcaggcc gagcacggtc
     4381 aggcgcacca ccggcgtgcc gccgaagatc atcgtggtga ccaccgcggc cggcttgccg
     4441 gtgccgaaga aggcgatcac cgggatcatg aagacgaagg ccggcatggt ctgacaaaaa
     4501 tccatgatcg gccgaatgaa ggcgtaaaaa cgttcacgcc gcgcgcagta catgccgagc
     4561 ggaatgccga tcacgatcga cagtaccgcg gcggtgccga gcagcgccag cgtggtcatg
     4621 gctttttccc agaagcccag caaccccatg tagagcagaa agccgcccga aaagatcgcc
     4681 gcacgcggtc ccgccgacag ccaggtcagc aggatgatga aactcgcgat caccatccac
     4741 ggggtttgca cgaagatcag ttcgagcgcg tccagcacgc tgcgaatcgc aaaggtgatg
     4801 gcatcgaaaa aggcttcacc gttggtcacg cagaaggcga agaattcctc gacgcctttg
     4861 atactgccga ggcgaatgcc gtgctcggtc ggaaaatcta tcagccacgg gtaggccccg
     4921 gggaagctgt agtgaatcac ggtcgcgatc atgatcaccg cgacgaaacc gcaactcagc
     4981 gccatgtcgc gcggcgtcat gccggtggaa atgtccgggt ccgacagcca gtccgaaaaa
     5041 cgcttttcca acagggtatt ggcaaccacc gactgtgccg ccttgaccag cagcagcacc
     5101 aggataccga ccagcgcgat cgccaacccg ctgtcctcca tttgctgggc ctcgaggcga
     5161 atcccgccaa tcgcgtcctc gagcgattcg accgtgcgcc ggtagacatc gaccttgtcc
     5221 gaattgctct ctatcgccgc cgccagttgc tggcgccgaa gctcgagcgt gccttcgatc
     5281 ttgccgatgc gctcccaggc ctcggcggcg agatcgccga acatgccgcg tataatctgt
     5341 accgcggcca gggtctcgag gatcaggaag gccaaccccc agttccacat gccgcgcgcg
     5401 ctgaaccaga tcgggccgaa gaagccggcc caggaattga aggtccagac gaatttcgaa
     5461 tcgccgccga tcttcgcgaa ctggctgcga tagtactcgg gattggtttt gacgaagcgc
     5521 tcgacctgcg ccgagcgctc ggcggccaaa tcctcgcttt cgcgcgcggc agcggcggta
     5581 atttcctcgg ccgccatatc ggttgccgta gcactcatga ggtttcagcc ccctcgatga
     5641 cggtttgcag tatgtcg
//
LOCUS       DATCZV010000600         3121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_579901, whole genome shotgun sequence.
ACCESSION   DATCZV010000600 DATCZV010000000
VERSION     DATCZV010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579901"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VIW27_13505"
     CDS             complement(<1..730)
                     /locus_tag="VIW27_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5740738.1"
                     /translation="MPDACSPAVSRRLASLLPLEVSGLEFVAGDEKLLDDIELSIDSS
                     GITVIMGPNGAGKSLLLRLIHGILQPASGQIRWQHRPMDAAIRHRQALVFQKPVLLRR
                     SVAANLDFVLKAGGGDYRERRDSLLRQAGLYAQRARPARLLSGGEQQKLALARALATE
                     PSVLMLDEPCASIDAASTWQIEERLLQVRQRGVKIILVTHDIGQARRLADDVVFMYHG
                     RVLEHSVAARFFEAPSCPEAAAYLD"
     gene            complement(723..1430)
                     /locus_tag="VIW27_13510"
     CDS             complement(723..1430)
                     /locus_tag="VIW27_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740739.1"
                     /translation="MQDFTAAFGLAFALIVDADADLIEIIGLSLRVSLSAVLLSALIG
                     FPLGAALAVSRFPGRQVLAVVLNALMGLPPVVVGLLVYMALSRAGPFGWLNLLYTPAA
                     MIIAQAILVTPIIAALTRQVIEDMHHEYDEQFRSLCLPTHRVIGALIWDARYSLLTVS
                     LAGFGRAIAEVGAVIIVGGNIDHLTRVMTTAIALETSKGDLALALALGFVLLLISLSV
                     NAGVYGLRSSARKLAYA"
     gene            1844..2527
                     /locus_tag="VIW27_13515"
     CDS             1844..2527
                     /locus_tag="VIW27_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5740740.1"
                     /translation="MIEIRNLGMSDTLEQGRLDVLDGVDLEIADGDTLAIIGPSGSGK
                     TTLLLLLAGLEQAQRGSIRIDGVDLAALDADGLADLRRDRLGIVFQSFHLVPSLTALG
                     NVSLPLEIAEQEGAGERAAAMLERVGLAERQHHYPMQLSGGEQQRVAIARALVHSPRL
                     LLADEPTGNLDLETGKRIIELLFDLNAASGSTLVLVTHDEQIASRCRRVLRLHAGKLI
                     EDQDHAFPA"
     gene            2511..>3121
                     /locus_tag="VIW27_13520"
     CDS             2511..>3121
                     /locus_tag="VIW27_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740741.1"
                     /translation="MRFLLELAWRDLRHGSRSLWVFCACLALGVTLVTASGGLHRLTS
                     HSLLQDTRKLVGGDLEVEASAPLPAEVLDWMRANGEISLVTELYTMLGGADGGFLRVE
                     LQSMDERYPLYGELELEPAAGLAEVTALRDGRWGIAIDPLLGVRLGLEVGDRVAIGGI
                     ETEVRALVRRQSDRALNADWRGPPVLLSAAALEASGLIQPGSRV"
BASE COUNT          560 a         1045 c         1008 g          508 t
ORIGIN      
        1 catcgagata agccgcggcc tcggggcacg acggcgcttc gaagaacctc gccgcgacgg
       61 aatgctcgag cacgcgcccg tggtacatga acaccacgtc atcggccagg cggcgtgcct
      121 ggccgatatc atgggtgacg agaatgatct tgacgccgcg ctgccgaacc tgcagcagcc
      181 gctcctcgat ctgccaggtg ctggcggcat cgatgctggc acagggttca tcgagcatca
      241 gtaccgacgg ttcggtagcc agcgccctcg ccagcgccag tttctgctgc tcgccgccgg
      301 acagtagccg ggcgggacgc gcgcgttgcg cgtaaagccc ggcctggcgc agcaggctgt
      361 cgcggcgttc gcggtaatcc ccgccgccgg ccttcaacac gaagtcgagg ttggccgcca
      421 ccgagcggcg cagcaacacc ggcttttgaa aaaccagcgc ctgtcggtgg cgaatcgcgg
      481 catccatcgg cctgtgctgc cagcgaatct ggccgcttgc cggttgcaga atgccgtgga
      541 tcaggcgcag cagcaggctc ttgccggcgc cgttcggccc catgattacg gtgatgccgc
      601 tgctgtcgat cgacagctcg atatcgtcga gcagcttttc gtcaccggca acgaattcga
      661 gtccgctgac ctcgagcggc agcagcgagg caaggcggcg ggacaccgct ggcgagcagg
      721 cgtcaggcat aggcgagttt cctcgcgctg gagcgcaaac cgtatacccc ggcattcacc
      781 gacagcgaaa tcaacagcag cacgaaaccg agcgccagcg ccagcgcgag atcgcctttc
      841 gaggtttcga gcgcgatcgc ggtggtcatc acgcgcgtca aatgatcgat gttgccgccg
      901 acgatgatta ccgcgccgac ctcggcgatg gcgcgcccga agccggccag gctgacggtt
      961 agcaggctgt agcgcgcatc ccagatcagc gcgccgatga cacggtgggt gggcaggcac
     1021 agcgaacgaa actgctcgtc gtactcgtga tgcatgtcct cgatgacctg gcgcgtgagc
     1081 gcggcgatga tcggggtcac gagtatggct tgcgcgatga tcatcgctgc cggcgtgtac
     1141 aacaggttca gccagccgaa cggcccggcg cgcgacagcg ccatgtacac cagcagaccg
     1201 accaccaccg gcggcaggcc catcaacgcg ttgagtacga ctgccaacac ctggcggccc
     1261 ggaaaccggg acaccgccag cgcggcgccg agcggaaagc cgatcagtgc cgacagcagt
     1321 accgcgctca ggctgacgcg cagcgacagg ccgatgattt cgatcaggtc ggcgtcggca
     1381 tcgacaatca gcgcgaaagc tagtccaaag gcagcggtga aatcttgcat gtcagaatta
     1441 tgtcttttac tgcatatttt tcgccatatt aataaatcga gtaatttatg caaataattg
     1501 atttcctgaa tgcgctcggc ggattcccgc gatcgcacct ctggcagcgc ccgccgaccg
     1561 gctgtcgccg gggcaagcca ggagccatag ctcgccgaca taaccgcttt ccctgcctgc
     1621 agcaacaggc ttcaacgcat gccggcgacc ggaacgacac ggctgcggtt cgacgagccg
     1681 caaaaccgcg gctggcgtta cctgaatcct gaatggtgca ggcttgcccg cagccggccg
     1741 agcccatatc atggccgctg ccgcaacttt gccgaatcat gcattgctca ttcgatagcc
     1801 gccgccgtca gcgcggtgga acctgcagac acgagttcca caagtgatcg agattcgcaa
     1861 tctcggcatg tccgacaccc tcgagcaggg gcgactcgac gtgctcgacg gcgtcgatct
     1921 cgagatcgcc gacggcgaca cgctggcgat catcgggccc tcgggttccg gcaagaccac
     1981 gctgctgctg ttgctcgccg gtctcgaaca ggcccagcgc ggcagcatac ggatcgacgg
     2041 cgtcgatctc gccgcactcg acgccgacgg cctcgccgac ctgcgacgcg accggctcgg
     2101 catcgttttc cagtcgtttc acctggtacc gagcctgacc gcgctcggca acgtctcgtt
     2161 gccgctggaa atcgctgaac aggagggtgc gggcgagcgt gccgcggcga tgctcgagcg
     2221 cgtcggcctc gccgaacggc agcatcatta cccgatgcag ctgtccggcg gcgaacagca
     2281 acgcgtcgcc atcgcgcggg cactggtgca ttcgccgcgc ctgctgctgg ccgacgagcc
     2341 gaccggtaac ctcgacctcg aaaccggcaa gcgcatcatc gaattgctgt tcgacctgaa
     2401 cgcggcgagc ggctcgacac tggttctcgt cactcacgac gaacaaatcg ccagtcgctg
     2461 ccgacgcgtg ctcaggctgc acgccggcaa gctgatcgaa gaccaggacc atgcgtttcc
     2521 tgcttgagct ggcctggagg gacctgcgcc atggcagccg ctcgctgtgg gtgttctgcg
     2581 cctgcctggc gctcggcgta acactggtca cggcctcggg cggccttcac cgcctgacca
     2641 gccacagcct gctgcaggac acccgcaagc tggtgggtgg cgatctcgaa gtcgaagcca
     2701 gcgcaccttt gcccgccgag gtgctcgact ggatgcgcgc caatggcgag atttcgctgg
     2761 tcaccgaact gtatacgatg ctcggcggcg ccgatggcgg ttttctgcgc gtcgaactgc
     2821 agtcgatgga cgagcgctac ccgctgtacg gcgaactcga gctcgagccg gccgccgggc
     2881 tggccgaggt caccgcgctg cgcgacggcc gctggggcat cgccatcgac ccgttgctcg
     2941 gcgtgcgcct cggcctcgag gtcggcgacc gggtcgcgat cggcggcatc gagaccgagg
     3001 tccgcgcgct ggtccgccgg cagtccgacc gcgcgctgaa cgccgactgg cgcggcccgc
     3061 cggtgctgct gtcggccgcg gcgctcgagg ccagcgggct gatacagccc ggcagccggg
     3121 t
//
LOCUS       DATCZV010000601         5841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_101314, whole genome shotgun sequence.
ACCESSION   DATCZV010000601 DATCZV010000000
VERSION     DATCZV010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101314"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1181)
                     /locus_tag="VIW27_13525"
     CDS             complement(273..1181)
                     /locus_tag="VIW27_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5740742.1"
                     /translation="MSLSLRHLRAFVEVARQGSFRRAAEKLYLSQPALTITINQFEEL
                     VGVTLLNRTTRRVGLTVDGENFLPIAERLVTDFERAMSDLKASAERRDGKVAIAVLPS
                     LTSHLLPGLLARFKDANPGIRVVLRDDNARGVQRQVLNNESDFGISNRWEEDSELEFT
                     PLTADPVGLVCPPDHPLGKSKRPIAWRKLQGLPFVGMSQDTGVNKLISAIEGCPESVR
                     SPEYEVLTMAALGGILEARLAITALPLLAVPSHMHPALVFRKLNNPRVERQLCLITRR
                     HYPLSTSAQKVHDMLLTETPRAVDQL"
     gene            complement(1222..2952)
                     /locus_tag="VIW27_13530"
     CDS             complement(1222..2952)
                     /locus_tag="VIW27_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5740743.1"
                     /translation="MRKYLHITLDDRSVDTRDLHGEALARSGRYRIASSLLELGAATV
                     EPLSPANPLIFCAGPFAGTNFSNANRISVGCKSPLTGGIKEANAGGNFALALGQMQLA
                     GLTLHGAADDWVVIHIAGDGEVSFAAADAYLGKGNVEAAAMLRQQYGEKVSLALCGPV
                     GEYQGLLAGIAFTDPDGRPSRMAARGGVGAVMGSKKVKAIVVDLHKMPPLHDRRKVMQ
                     AVREYGARLREQEAVRNLNRLGTAYVADFTNHVGGLPVRNFSAGRLVDTQIEPLKMGG
                     DYLREQNLERGGEPAHACMPGCIIECSNVYADADGNEVVSPLEYETIGLLGSNCGLED
                     PDELAALNAVANDLGIDSIETGATLAVLMEAGLAGFGDAGFMRRALEEIRRGSERGKL
                     WARGTARVGEHYGVERVPVIKRQAISAYDPRVIEVTGITMMLTAQGADHTAGNVPMMD
                     CSGKQAAELVAASLQAQVSSAAADSLGLCIFGRSVTDTSTELVTQAINDAHGTEIEPS
                     FFWELGRETLRLEHEFNRAAGFLAADDELPEFFHDEPLTPSGKVARFDCREIHRCVNE
                     WRQAHMTRSG"
     gene            complement(2971..3312)
                     /locus_tag="VIW27_13535"
     CDS             complement(2971..3312)
                     /locus_tag="VIW27_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY5740744.1"
                     /translation="MLALCANYEGTVREDPESFDHYVETVHLPLVARYPRLRALRYMK
                     GVARDGVPPKYYLSFELFFDSLEDFQVAAQSEQRDRAVEDAKKIAARFDGEIHHVMYA
                     VNDIPIDADQT"
     gene            complement(3328..3798)
                     /locus_tag="VIW27_13540"
     CDS             complement(3328..3798)
                     /locus_tag="VIW27_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5740745.1"
                     /translation="MAGTIEARLVELGIELPDAPAPVSNYMPYVVSGNLVFISGQVTL
                     WNGEIQFIGKIGADMSVEQGYEAARICGLNQIAQVRAACGGDLDRVSKVVKLGAFVNC
                     VDGFTDLPQVVNGASDLMIEVFGDAGRCTRTNVGVSSLPLGFAVEIDGIYEISR"
     gene            4141..5364
                     /locus_tag="VIW27_13545"
     CDS             4141..5364
                     /locus_tag="VIW27_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY5740746.1"
                     /translation="MADLSTEFCGLKFKNPVVVASAEPGNSVDNMKKCIDHDAGGIII
                     KTIGDLPGMQTLTKHSKYAILNDRGEPIQGKVNRNFFFYSRSGYVSEPYQEWIPILAE
                     SQRYAQQNGAHIIGNIASGTIDGWVMLAKMLEDCGLPLIELNYQCPHPVDYAGPTEGI
                     WIAQDPNMTAELTERILEAVDIPVMVKLTPETHNLTDIARAARDAGAASVAVNSRFVG
                     FAVDIDNARPYIDGVAGIGGPWVKYMTLRWIHEIYSKLGIPMSASNGIYDGRDAIEFF
                     MTGARIMQVCSVLMLKGIEWLPKIIAGMDKFLDQHGYPDLESIYGIASRRSKTPAELF
                     EAPPVYSLVDHDKCKTPRCVICAKVCFYDAMHIVDERMVVDAEKCIGCELCQSVCAFG
                     AISLTGDKQRVALNA"
     gene            5417..>5841
                     /locus_tag="VIW27_13550"
     CDS             5417..>5841
                     /locus_tag="VIW27_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate flavodoxin/ferredoxin oxidoreductase"
                     /protein_id="HEY5740747.1"
                     /translation="MGLEFLEGNEAIARGAMQANCNFFAGYPITPSSSILQHMLELVP
                     QRGGVAVQAEDEIASIGFCIGAAMTGSKVLTATSGPGISLYSENIGLAIIGETPMVIV
                     NVQRQGPATGSATKGAEGDIQFTRWCTSGGLPVIVLSPAT"
BASE COUNT         1027 a         1832 c         1865 g         1117 t
ORIGIN      
        1 ggtgccatcg agcgggccga tgggcgtcat ggcacgggta tcgatcatct cgttacgcgt
       61 caatccctcg gttgttttgg gtgtcttgca ccgggatgca agccctggaa cctgctgcag
      121 gcgaaaaccg cgaggccgcc cgcggttttc gaagcgcgaa ggtcaattac tactccgatc
      181 gtcgtgccgc gtcaacctcg agcgtgatcg ccggcgagcg atcggcaaac gcggctgcgt
      241 cgattgccct gtcgccgcac ggacatgcgg gttcacagct gatcgaccgc gcgcggagtc
      301 tcggtgagca gcatgtcgtg aaccttttgc gcggaggtcg acagcgggta gtgtcgccgc
      361 gtgatcaggc aaagttgccg ttctacccgc gggttgttga gtttgcggaa caccagcgcc
      421 ggatgcatgt ggctgggtac ggcaagcagc ggcagggcgg tgatcgccag cctcgcttcc
      481 agtattccgc cgagcgcggc catcgtcagc acttcgtatt ccggggagcg gacggattcg
      541 gggcaaccct cgattgcact gatcagcttg ttgaccccgg tgtcctgcga catgccgacg
      601 aatggcaggc cctgcagttt gcgccaggcg atcggccgct tgctcttgcc gagcggatga
      661 tccggcggac agaccaggcc gaccgggtcg gcggtcagcg gtgtgaactc gagttccgaa
      721 tcctcttccc agcgattgct gatgccgaag tcggactcgt tgttgagcac ctggcgctgt
      781 accccgcgcg cattgtcgtc gcgcagcacg acgcgtattc ccgggttggc atccttgaac
      841 cgggccagta acccgggcag caggtggctg gtaagcgacg gcagcacggc gatcgccact
      901 ttaccgtcgc ggcgttcggc cgacgccttc aggtcggaca tggcgcgctc gaaatcggtt
      961 accaggcgtt cggcgatcgg caggaaattt tcgccgtcga cggtcaggcc tacccgccgc
     1021 gtggtgcgat tcaacagtgt gacgcccacc agttcctcga actggttgat ggtgatcgtc
     1081 agcgccggct gggacagata aagtttttcc gccgcacggc ggaagctgcc ctggcgggcc
     1141 acctcgacga aggcgcgcag atgtctcagg ctgaggctca agggcggact ccgccgctcg
     1201 aacccggccg cccggttgcg ctcaaccgga tcgggtcatg tgcgcctgcc gccactcgtt
     1261 tacgcatcga tggatctccc ggcaatcgaa gcgagctacc ttgccgctcg gcgtgagtgg
     1321 ctcgtcgtgg aaaaattcgg gcagctcatc gtcagcggcc aggaaacccg ccgcccggtt
     1381 gaactcgtgc tccaatctta gcgtttcgcg gcccaattcc caaaagaaag atggctcgat
     1441 ctcggtgccg tgagcgtcgt tgatcgcctg ggtgaccagt tcggtactgg tgtcggtcac
     1501 cgagcggccg aagatgcaca accccagcga atcggccgcc gcgctgctga cctgggcctg
     1561 caggctggcg gccaccagtt cggcggcctg cttgccgctg caatccatca tcggcacgtt
     1621 gccggcggta tggtcggcgc cctgggcggt cagcatcatc gtgatccccg tcacctcgat
     1681 gacgcgcggg tcgtaggcgc tgatcgcctg ccgcttgatc accggtaccc gctcgacccc
     1741 gtagtgttcg ccgacgcggg ccgttccccg tgcccatagc ttgccgcgct cgctgccgcg
     1801 gcggatttcc tcgagcgctc ggcgcatgaa gccggcgtcg ccgaagccgg cgagaccggc
     1861 ctccatcagc accgccagcg tggcgccggt ttcgatgctg tcgatgccga ggtcgttggc
     1921 gaccgcgttg agcgcggcca gctcgtccgg gtcctcgaga ccgcagttgc tgcccagcag
     1981 gccgatggtt tcgtactcga gcggcgaaac cacctcgttg ccgtcggcgt cggcgtaaac
     2041 gttgctgcac tcgatgatgc agcccggcat acaggcgtgc gccggctcgc cgcccctttc
     2101 gagattctgc tcgcgcaggt aatcgccgcc cattttcagt ggttcgatct gcgtatcgac
     2161 cagtcgtccg gcgctgaaat tgcgtaccgg caggccgccg acgtggttgg taaagtcggc
     2221 cacgtaggcg gtacccagcc ggttcaggtt gcggaccgcc tcctgttcgc gcagcctggc
     2281 gccgtattcg cgcaccgcct gcatgacttt cctgcggtcg tgcagcggcg gcatcttgtg
     2341 tagatcgacg acgatggcct tgaccttctt gctgcccatc accgcgccaa cgccgccgcg
     2401 ggcggccatg cgcgacgggc gcccgtcggg atcggtgaag gcgattccgg ccagcagacc
     2461 ctggtactcg ccgaccggtc cgcacagggc caggctgact ttttcaccgt attgctgccg
     2521 cagcatggcg gcggcctcga cgttgccctt gcccaggtag gcgtcggcag cggcgaagct
     2581 gacctcgccg tcgccggcga tgtggatcac cacccagtcg tccgcggcgc cgtgcagcgt
     2641 caggccggcc agttgcattt gtcccagtgc gagcgcgaaa ttgccgccgg cgttggcttc
     2701 cttgatgcca ccggtcagcg ggctcttgca gcccacgctg atgcggttgg cgttggagaa
     2761 attggtaccg gcgaacggcc cggcgcaaaa gatcagcggg ttggcgggcg acagcggttc
     2821 gaccgtcgcc gcgccgagtt cgagcaggct gctcgcgatg cggtagcgtc cgctgcgggc
     2881 gagggcttca ccgtgcagat cccgcgtgtc gaccgatcgg tcatcgagag tgatatgcag
     2941 atatttgcgc atcgggcacc tgtggcgatc tcaagtttgg tcggcgtcga tcgggatgtc
     3001 gttcaccgcg tacatgacgt ggtggatttc gccgtcgaag cgcgcggcga ttttcttcgc
     3061 gtcctcgacc gcgcggtcgc gttgctccga ttgcgcggcc acctggaagt cctccaggct
     3121 gtcgaaaaac agttcgaagg acaggtagta ttttggcggc acgccgtcac gcgccactcc
     3181 cttcatgtag cgcagcgccc gcagtcgcgg gtacctggcg accagtggca gatggacggt
     3241 ctcgacgtag tgatcgaagc tttccgggtc ttcgcggacg gtgccttcgt agttggcgca
     3301 cagggctagc atcgcgttac ctcccggtta gcggctgatt tcgtaaatgc cgtcgatttc
     3361 gacggcgaaa cccagcggca gcgatgacac gccgacattg gtgcgcgtgc agcggccggc
     3421 gtcgccgaac acttcgatca tcaggtcgga ggcgccgttg accacctgcg gcaggtcggt
     3481 aaagccgtcg acgcaattga cgaaggcgcc gagcttgacg accttgctga cgcggtcgag
     3541 gtcaccgccg caggcggcgc gcacctgggc gatctggttg aggccgcaga tgcgtgccgc
     3601 ctcgtagccc tgctcgaccg acatatcggc gccgatcttg ccgataaact gaatctcgcc
     3661 gttccacagc gtaacctggc ccgagatgaa caccaggttg ccgctgacca cgtagggcat
     3721 gtagttggat accggtgcgg gggcgtcggg aagttcgatt cccagttcga ctagacgtgc
     3781 ttcgatcgtg ccggccatat cgaggtctcc tctgcgtttc gccgcgcggg tgcggccgcg
     3841 ggttatcggt aaagaatgca gcgatgctaa cgaccgacat gctctttagt caatttaaat
     3901 aaaacgatca actattaatc tatacttatc aatctgaatc agcggcgcgc aggcccggaa
     3961 aacgcactcg atccgcgcgc cggcggcata tttcaattga taactttttt aaaagaattc
     4021 tttatttctt ttgattggac atatcaatcg gcacagtcct agaattttac gatacccacg
     4081 gtccgccgcg aatacgcgcg ggtgtcaccg gggcgtgcag aatctggcag gagcaacgct
     4141 atggctgatc tgagcaccga attttgcgga ttgaagttca agaacccggt cgtcgtcgca
     4201 tcggcggagc cgggcaacag cgtcgacaac atgaagaagt gtatcgacca cgacgccggc
     4261 ggcatcatca tcaagaccat cggcgatctt cccggcatgc agacgctgac gaagcattcc
     4321 aagtacgcca ttctcaacga tcggggcgag ccgattcagg gcaaggtcaa ccgcaacttt
     4381 ttcttctaca gccgctcggg ttacgtcagc gagccctacc aggagtggat tccgatcctg
     4441 gcggaaagcc agcggtatgc gcagcaaaat ggcgcgcaca tcatcggcaa tatcgcctcg
     4501 ggcacgatcg acggctgggt gatgctggcg aagatgctgg aggattgcgg cctgccgctg
     4561 atcgagctca attaccagtg cccgcatccg gtcgattacg cgggcccgac cgagggcatc
     4621 tggatcgccc aggacccgaa catgaccgcc gagctgaccg agcgcatcct cgaggcggtc
     4681 gatattccgg tgatggtcaa gctgaccccg gaaacccaca acctcaccga tatcgccagg
     4741 gcggcgcgcg atgccggcgc cgcgtcggtc gcggtcaaca gccgcttcgt cggctttgcc
     4801 gtcgatatcg acaatgccag gccctacatc gatggcgtcg ccggcatcgg cggaccgtgg
     4861 gtcaagtaca tgacgctgcg ctggatccac gaaatctaca gcaagctcgg cattcccatg
     4921 tccgcttcca acggcatcta cgacgggcgc gacgcgatcg agtttttcat gaccggcgcg
     4981 cgcatcatgc aggtctgctc ggtgctgatg ctgaagggga tcgaatggct gccgaagatc
     5041 atcgccggca tggacaagtt tctcgaccag cacggttacc cggacctcga atcgatatac
     5101 ggcatcgcct cgcgtcgcag caagaccccg gcggaactgt tcgaggcgcc gccggtgtat
     5161 tcgctggtcg atcacgacaa gtgcaagaca ccgcgctgcg tgatctgcgc caaggtgtgt
     5221 ttttacgatg ccatgcatat cgtcgacgag cgcatggtcg tcgatgcgga aaaatgtatc
     5281 ggctgcgaac tgtgccagag cgtctgcgca ttcggcgcga tcagcctgac cggggacaag
     5341 cagcgggtgg cactgaatgc atgaatgccg ccgctacccg ccgcatgccc acggcaacca
     5401 ctgacaggag ctgctgatgg gactcgaatt tctcgaaggc aacgaggcga tcgcccgcgg
     5461 tgcgatgcag gcgaactgca atttcttcgc cggctacccg attacgccgt cgtcgtcgat
     5521 attgcagcac atgctggagc tggtgccgca gcgcggcggt gtggcggtcc aggcggagga
     5581 cgagatcgcc tccatcggct tttgcatcgg cgcggcgatg accggcagca aggtgctgac
     5641 cgccaccagc ggccccggca tcagcctgta cagcgaaaac atcggcctcg ccatcatcgg
     5701 cgaaacgccg atggtcatcg tcaacgtgca gcgccaggga ccagccaccg gttccgccac
     5761 caagggcgct gaaggcgata tccagttcac tcgctggtgt acctcgggcg ggctgccggt
     5821 gatcgtgctg agcccggcca c
//
LOCUS       DATCZV010000602         8670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_207886, whole genome shotgun sequence.
ACCESSION   DATCZV010000602 DATCZV010000000
VERSION     DATCZV010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207886"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..789
                     /locus_tag="VIW27_13555"
     CDS             37..789
                     /locus_tag="VIW27_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5740748.1"
                     /translation="MNAFQQFFYDLAQEHPKWNFIYFYDPTQTDRVIEGLWMTLQLSV
                     IFVILSVIIGVVGAWGQGAQSKTLRLALQGYIQFFRNTPPLVQLMFFYFALGQFTPTY
                     SPDGWLEVPIISNVGWAIISLSFFAGAFNVEIFRAGIEAVPDSTKEAAEALGMNRLQI
                     YIYVVLPLAFRVCLPALNNNLVNLVKTTTQALAIAVPELLYQMISIYNDYSTAQNACM
                     VFLFFAYIILVGILVFGMHRWERAMWIPGYGR"
     gene            786..1667
                     /locus_tag="VIW27_13560"
     CDS             786..1667
                     /locus_tag="VIW27_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5740749.1"
                     /translation="MKRNPLTTNTDLAVLKPFDYSPAGTVLDLQFSRWLAEFSPRHLL
                     LVFALFCLWPAIALAQSNYSYGEALEALWRWLPFVVGQGFVLNLVVSILTMLIGTVAG
                     VLLGLGQISPLRPLAWTCWFVTQLFRNSPWLVLLFIVMLAFPFEIQIGDTIIQIPAWM
                     KAVFGLSLPIMANISEIVRGAIASVPTGQWEAADSLAYSRGQTLWLVILPQCFKRMIP
                     PWMNWYAILTMATPLISLLGVEEIITLSRQAMEAEDNHPELLVPFFGFALIIFFAYCY
                     PIARWTIRLEKRFVLRQ"
     gene            1686..2441
                     /locus_tag="VIW27_13565"
     CDS             1686..2441
                     /locus_tag="VIW27_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY5740750.1"
                     /translation="MTAADWTPDQPIVSIKDVHKSFGDLEVLKGVSLDVMKGQVICII
                     GPSGSGKSTLIRCVNALNTIDSGSIKVEGLEVNDPTLDKLELRKTVGMVFQQYNLFPH
                     KTALENVMMAPIKVLKQPPDEVEEYARKLIKRVNLVGKENAYPGELSGGQQQRVAIAR
                     SLAMHPDVMLFDEVTAALDPETVNEVLETIKDLAQRGMTCLLVTHEMGFAREIADHIY
                     FTDRGVIVEHGPPATFFKEAKDPRTQEFLSQIL"
     gene            2467..4893
                     /locus_tag="VIW27_13570"
     CDS             2467..4893
                     /locus_tag="VIW27_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011570164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740751.1"
                     /translation="MTGNTELPAEASVVVIGGGVMGCSTLYHLARAGVGDALLLERNQ
                     LTSGTTWHSAAQVRALRSSKNLTDLIRYSISLYASLEAETGQATGWINKGSLSIATNR
                     DRLTFIRRQEALARLYGVNAESISPAAAAERWPMMNCDDVIGAVWSPDDGRVGPSDLC
                     AALVKGATARGARIFENTPVTGISTRGGKIAGVMTPAGEIRCDAIALCSGLWSRENAA
                     MAGVDAPLWPCEHFYLLTNPIEGISGNLPTLSDHDSHLYIRDDSGGLLVGCFEPQGKP
                     IDPRRIGEDFAFQLLPEDWDHFEPMLMNAMHRLPCLADAEIRMLLNGPESFTPDGSFM
                     LGESAETRGLFLGCGMNSVGVASGGGAGMALAHCIIHGHTPTDLHEADPKRFPSCFNS
                     ARALSERVPEILGTHYAVNYPGMQLKSARNLKLLPLHRQWQRHEAYFGQFFGWERPLY
                     FDAPGEPRLGFGKPDWFDQVEREVHAAHTGAALFDQSSFGKIRVSGSDAERFLDRVCA
                     NNMRRQPGRVIYTAMLNQRGCYESDLTALRLGADEYRLYTGSGAIRRDLAWLRRHLQP
                     GQRVAISESTDDFAVLALMGPQAVSIAAQFDAHDLLALGYFEHCRARIAGIEVDAARL
                     SYVGEAGWELTCRTVDAQTLGELLLEAGATPAGVFAQTAMRIEKRFLAYGHDLDTDID
                     PLQAGLDFGIDWHSDFIGKPALLERRRRLCTRRLLNIVFDSADACPIGNEPVYLQNRI
                     IGKTTSAAFGYRLGYPVAIATLDLPDGTDPDGLEVDVDIARDQNSARVLTKPAFDPDG
                     SRMRLAPRST"
     gene            4890..6218
                     /locus_tag="VIW27_13575"
     CDS             4890..6218
                     /locus_tag="VIW27_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5740752.1"
                     /translation="MSKVFPRNCKASFPIAVEGDGVYIVDRDGKRYFDGSGGAAVSCL
                     GHGDRDINAAIEAQLERLEFAHTGFFSSEPAEALADLLIEHAPGTLDRVYFVSGGSEA
                     VEAAIKLARQYFLETGQPSRHRVIARRQSYHGNTLGALAAGGNLWRREPFAPLMIDTT
                     HVSPCYAYRDQQADETAADYGRRVADELEAEILRLGEDQVMAFLAEPVVGATLGAVPA
                     VDGYFRRIREICDRYGVLLILDEVMCGMGRTGTLYACEQEGIAPDIMTIAKGLGAGYQ
                     PIGAMLCTDTIYRAIEQGSGFFQHGHTYIGHPVACAAGHAVVSKITGQGLLDNVITRG
                     AALREALQQRFGEHPHIGDIRGRGLFLGMEFVQRRDTRETFDPGLGFHKRFKKAAMEA
                     GLICYPMGGTIDGQHGDHVLLAPPFIIDDNHVSEIVDKLDAALGTVLKSL"
     gene            6215..7036
                     /locus_tag="VIW27_13580"
     CDS             6215..7036
                     /locus_tag="VIW27_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY5740753.1"
                     /translation="MTREVVIMVAPNGARKTKADHASLPVSIADTVDEAERCFAAGAS
                     ALHGHVRGAADEHVLDAGRYRELIAEMTRRTPEMLLQITTEAIGRYTPAQQAACVRAV
                     EPAMVSVILKEISSNFEDSDFARDFFACCDDHRVHVQHILFSAEELDGFLRLRDQRVI
                     PAGHRCVLFVLGRYSVDFQSAPEDLEPFLQRDLAGLDWFTCAFGRREQDCVLAAVEAG
                     GHARIGFENNLYLPDGEIAADTAALVTSLAAALRATGRRPASAAAARQLLGVRSA"
     gene            complement(7009..8232)
                     /locus_tag="VIW27_13585"
     CDS             complement(7009..8232)
                     /locus_tag="VIW27_13585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740754.1"
                     /translation="MLVDYWRFARANPRFLGFGFFLAFLSSAGQTYFVGVFGTAIQAD
                     FGLDPGSWGRTYMIGTLASALVINWSGSLIDRFDLRIFTVATLAGLAAACLVMGSVTS
                     PLLLAAAIFLLRQFGQGLTSHTGITSMARYHDADRGKAIALAAIGFSCGEAVLPLAGL
                     YAAQAIGWRMTFHAVAVCVLAAILPALWLLKGHGRRHAEHSAALERRAAETGGMADYT
                     RRQMLSETRFYLMLPAMISPSMIGTALFFFPAEIAAAKHWSSLWVTGNYWLYSTVSVA
                     TTIYSGLLIDRFGARRVVPLFLLPLAVALVVLNLSDQHWIIWPYMLLMGLSTGLYFTG
                     LSALWAELYGARHLGAIKSLTNAVMVFASALGPALVGTLLEWDVAFAAISILLAAFCL
                     LATALLAYALRTPSS"
     gene            8414..>8670
                     /locus_tag="VIW27_13590"
     CDS             8414..>8670
                     /locus_tag="VIW27_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5740755.1"
                     /translation="MAEIHPRLIADCIPLVRLPLCHLLLMNDSNFPWFILVPDREGIR
                     EIYQLDDADRAQLLRESCQLGEFLMRHFAGDKLNVAALGNQV"
BASE COUNT         1620 a         2845 c         2649 g         1556 t
ORIGIN      
        1 aagcgcgctt cggccggtcg gggtctggct ataccgatga acgcttttca gcagttcttc
       61 tacgatctgg cgcaggagca tccgaagtgg aatttcatct acttctacga tccgacgcag
      121 accgaccgcg tcatcgaggg cctgtggatg accctccagc tgtcggtaat cttcgtgata
      181 ctgtcagtaa tcatcggcgt cgtcggcgcc tggggccagg gcgcacagtc taaaaccctg
      241 cgccttgcgc tgcagggcta tatccagttt ttccgcaata ccccgccgct ggtgcagttg
      301 atgtttttct attttgcact gggccagttc acgccgacct attcgccaga cggctggctc
      361 gaagtgccga tcatctccaa cgtcggctgg gcgattattt cgctgtcgtt cttcgccggt
      421 gcgttcaacg tcgagatctt ccgcgccggc atcgaggcgg taccggactc gaccaaggaa
      481 gcggccgagg cgctcggcat gaaccggctg caaatctaca tctatgtcgt gctgccgctc
      541 gcgtttcggg tttgtctgcc cgcactgaac aacaatctcg tcaacctggt caaaaccacg
      601 acccaggcgc tggcgatcgc ggtaccggag ctgctctacc agatgatcag catctacaac
      661 gattactcga ccgcgcaaaa tgcctgtatg gtcttcctgt ttttcgccta catcatcctc
      721 gtcggcatac tcgtgttcgg tatgcaccgc tgggaacgcg cgatgtggat tccggggtac
      781 ggccggtgaa gcgcaacccg ttgacaacca acacggatct cgccgtgctg aagcccttcg
      841 actacagccc ggcaggcacc gtgctcgacc tgcagttcag ccgctggctg gccgagtttt
      901 cgccacgcca cctgctgctg gtgttcgcgc tcttttgcct gtggcccgca atcgcgctgg
      961 cgcaaagcaa ttacagctac ggcgaggcgc tcgaggcgtt gtggcgctgg ctgcccttcg
     1021 tcgtcggcca gggcttcgtg ctcaacctcg tggtcagcat tctgacgatg ctgatcggca
     1081 ccgtcgccgg cgtgctgctc ggcctcggcc agatatcgcc gcttaggccg ctggcctgga
     1141 cctgctggtt cgtcacccag ctgtttcgta attcaccctg gctggtgctg ctgttcatcg
     1201 tcatgctcgc ctttcctttc gaaatacaga ttggtgacac gatcatccag atcccggcct
     1261 ggatgaaggc cgtgttcggc ctgtcgctgc cgatcatggc gaatatttcg gaaatcgtgc
     1321 gcggcgcgat cgcgtcggtg ccgaccgggc aatgggaggc cgccgattcg ctggcctata
     1381 gccgtggcca gacactgtgg ctggtgatcc tgccgcaatg cttcaagcgc atgattccgc
     1441 catggatgaa ctggtacgcg attctgacca tggcaacacc actgatctcg ttgcttggtg
     1501 tcgaggaaat catcaccctg tcgcgccagg cgatggaggc cgaggacaat catccggagt
     1561 tgctggtgcc cttcttcggt ttcgcgctga tcatcttttt cgcctattgc tacccgattg
     1621 cgcgctggac gattcgcctg gagaaacgct ttgtactcag acaataacca atcggagacg
     1681 agccgatgac cgcagcagac tggaccccgg atcagcccat cgtttcgatc aaggacgtgc
     1741 acaagtcttt cggcgacctc gaagtactca agggcgtttc gctggatgtt atgaaaggcc
     1801 aggtcatttg cataatcggc ccctcgggct cgggaaaatc gacgctgatt cgttgcgtca
     1861 acgcactcaa cactatcgac agcggttcga tcaaggtcga ggggctcgag gtcaacgacc
     1921 cgacgctcga caagcttgaa ctgcgcaaga cggtcggcat ggtgtttcag caatacaacc
     1981 tgtttccgca caagacagcg ctcgaaaacg tcatgatggc gccgatcaag gtactcaagc
     2041 agccccccga cgaggtcgag gagtatgctc gcaaactgat caagcgagtg aatctcgtcg
     2101 gtaaggaaaa tgcttatccg ggtgaattgt ccggcgggca acagcagcgc gtcgcgatcg
     2161 cgcgatcgct ggcgatgcat cccgacgtga tgttgttcga cgaggtcacc gcggcgctcg
     2221 atccggaaac cgtcaacgaa gtgctggaga cgatcaagga cctcgcgcaa cgcggcatga
     2281 cctgcctgct ggtcacccac gaaatgggat tcgcgcggga aatcgccgac cacatctact
     2341 tcaccgaccg cggcgtcatc gtcgagcacg ggccgcccgc taccttcttc aaggaagcga
     2401 aggatccgcg cacgcaagaa tttctgagcc agatactcta ggcggccagc gactcgcgcc
     2461 ggcgtcatga ctggcaacac cgaactgccc gcagaggctt ccgtggtggt tatcggcggc
     2521 ggcgtcatgg gctgctccac gctctatcat ctggcgcgcg ccggggtcgg cgatgcgctg
     2581 ctgctggaac gcaaccagct gacctcgggg acgacctggc attcggccgc gcaagtgcgc
     2641 gcgctgcgct cgagcaagaa cctgaccgac ctgatccgtt actcgatatc gctatacgcg
     2701 tccctcgaag cagaaaccgg ccaggccacc ggctggatca acaagggatc gttatcgatc
     2761 gcgaccaacc gcgatcggct gactttcatt cgccgccagg aagcactggc ccgcctgtac
     2821 ggcgtcaacg ccgaatcgat ttcgcccgcc gcggccgccg agcgctggcc gatgatgaat
     2881 tgcgacgacg tcatcggcgc ggtctggtcg ccggacgacg ggcgcgtcgg tccgtccgac
     2941 ctgtgcgcgg cactggtcaa gggcgcgacc gccaggggcg cccgaatatt cgaaaacaca
     3001 cccgtcaccg ggatcagcac acgcggcggc aagattgccg gcgtgatgac cccagccggc
     3061 gagattcgct gtgatgctat cgctctgtgc tccggcctgt ggagccgcga gaacgccgcg
     3121 atggcgggcg tcgatgcgcc gctctggccc tgcgaacact tttacctgct gaccaatccg
     3181 atcgaaggta tatccggcaa cctgcccacg ctgtcggacc acgacagcca cctctatatt
     3241 cgtgacgatt ccggcggcct gctggtcggc tgcttcgaac cgcagggcaa gccgatcgat
     3301 ccgcgacgaa tcggcgagga tttcgcgttt cagctgttgc ccgaggactg ggatcatttc
     3361 gaacccatgc tgatgaacgc catgcaccgc ctgccctgcc tggcggacgc cgaaatccgt
     3421 atgctgttga acgggccgga gagtttcacc ccggacggct cgttcatgct cggcgaaagc
     3481 gccgagaccc gcggcctgtt tctcggctgc ggcatgaatt cggtgggtgt cgccagcggc
     3541 ggcggcgccg gcatggcgct ggcgcattgc atcattcacg gccatacgcc gaccgacctg
     3601 cacgaggccg acccgaagcg gtttccgtcc tgcttcaatt ccgcgcgggc gctcagcgag
     3661 cgcgtgccgg agattctcgg cacccactac gcggtcaact acccgggaat gcagctgaaa
     3721 tcggcgcgca atctgaaact cctgccgctg caccggcaat ggcaacggca cgaagcgtat
     3781 ttcggccaat tcttcggctg ggaacggccg ctctacttcg acgcgcccgg cgagccgcgg
     3841 ctcggtttcg gcaagcccga ctggttcgac caggtagaac gtgaggtgca tgcagcgcat
     3901 accggcgccg cactgttcga tcagtcgagc ttcggcaaaa ttcgagtcag cgggagcgac
     3961 gcggagcgtt tcctcgaccg cgtctgcgcc aacaacatgc ggcgccaacc cggccgtgtg
     4021 atttataccg cgatgctcaa ccagcgcggc tgctacgaga gcgacctgac cgcgctgcgc
     4081 ctcggcgcgg acgagtatcg cctctacacc ggcagcggcg cgattcggcg cgacctggcc
     4141 tggctgcggc ggcacctgca gcccggccaa cgggtggcga tctcggagtc gaccgacgat
     4201 ttcgccgtac tcgcactgat gggaccgcaa gcggtttcga ttgcggccca attcgacgca
     4261 cacgacctgc ttgcgctcgg ctatttcgaa cattgtcgcg cccgcatcgc cggcatcgag
     4321 gtcgacgccg cgcgcctgtc ctacgtcggc gaggccggct gggaactgac ctgccgcacc
     4381 gtggatgcgc aaaccctcgg cgagctgctg ctcgaagccg gcgcgacacc cgccggtgtt
     4441 tttgcgcaaa ccgcgatgcg catcgagaag cgctttctcg cttacgggca cgatctggat
     4501 accgatatcg atccgctaca ggcgggactc gacttcggca tcgactggca cagcgacttt
     4561 atcggcaagc ccgccctgct cgagcgccgt cggcgactat gcaccaggcg actgctgaat
     4621 atcgttttcg attcggccga cgcctgcccg atcggtaacg agccggtcta cctgcaaaac
     4681 cggattatcg gtaaaaccac ctcggccgcg ttcggttacc ggctcggcta cccggtggcg
     4741 atcgccacgc tcgacctgcc cgacgggacc gatcccgatg ggcttgaagt tgacgtcgat
     4801 atagcgcggg atcaaaattc ggccagagtg ttaacaaaac ccgctttcga tcccgacgga
     4861 tcacgcatgc gcctggcgcc gaggtcaaca tgagcaaggt atttccccgt aactgcaagg
     4921 ccagcttccc gatcgcggtc gagggcgacg gagtctatat cgtcgaccgc gacggcaaac
     4981 gttatttcga cggttccggc ggcgcggccg tctcctgcct cggtcacggc gaccgcgaca
     5041 tcaacgccgc gatcgaggcc cagctcgagc ggctcgaatt cgcgcacacc ggctttttca
     5101 gcagcgaacc agccgaggcg ctcgccgatt tattgatcga gcatgcgccg gggacgctcg
     5161 atcgcgttta cttcgtctcc ggtggatccg aggcggtgga ggcggcgatc aagctggcgc
     5221 gccagtactt cctcgagacc gggcagccct cgcgccaccg cgtgattgcg cggcgccagt
     5281 cctaccacgg caacacgctc ggcgcactgg ccgccggcgg caacctgtgg cgccgcgaac
     5341 ccttcgcgcc gctgatgatc gataccacgc atgtttcgcc ctgttacgct taccgcgacc
     5401 agcaagcgga tgaaactgcg gccgactacg gccggcgcgt tgccgatgaa ctcgaggccg
     5461 aaatcctgcg tctcggcgaa gaccaggtga tggcattcct cgccgagccg gtggtcggcg
     5521 cgacgctcgg cgcggtgcct gccgtcgacg gatatttcag gcgcattcgc gagatctgcg
     5581 atcgctatgg cgtactgctg atcctcgacg aggtcatgtg cggcatgggg cggaccggca
     5641 cgctctacgc ctgcgaacag gagggcatcg ccccggatat catgaccatc gccaaggggc
     5701 tcggtgccgg ttatcagccg atcggcgcga tgctgtgtac cgacaccatc taccgggcca
     5761 tcgaacaggg ttcgggcttc ttccagcacg gccacaccta catcggccac ccggtcgcct
     5821 gcgccgccgg ccacgcggtg gtcagcaaga tcaccggcca gggtctgctc gacaatgtga
     5881 tcacccgcgg cgcggcactg cgcgaggcgc tgcagcagcg attcggcgag cacccgcata
     5941 tcggcgacat tcgcggccgc ggcctgtttc tgggcatgga gttcgtgcag cgacgcgata
     6001 cccgcgaaac cttcgatccc ggactcggtt tccacaagcg tttcaagaag gcggccatgg
     6061 aggcgggcct gatctgctac ccgatgggcg gcaccatcga cggccagcac ggcgaccatg
     6121 tgttgctggc gccgccgttc atcatcgacg acaaccatgt cagcgaaatc gtcgacaagc
     6181 tcgacgccgc cctcggcacg gtattgaaat ccctgtgacg cgcgaagtcg tcatcatggt
     6241 ggcgcccaac ggcgcacgca agaccaaagc ggaccacgcc agcctgccgg tatcgatcgc
     6301 cgacacggtc gacgaggccg agcgctgctt tgccgccggc gccagtgcac tacacggcca
     6361 cgtacgcggc gctgccgacg aacacgtcct cgatgccggg cgctatcgcg agctgatcgc
     6421 cgaaatgacg cgccgcacgc cggaaatgct gctgcagatc accaccgagg cgatcggccg
     6481 ttacacgccc gcgcaacagg ccgcttgtgt tcgcgccgtc gaaccggcga tggtgagcgt
     6541 gatcctcaaa gagatcagtt cgaatttcga ggattccgat ttcgcgcgcg actttttcgc
     6601 ctgctgcgac gaccaccgcg tgcacgtgca gcatattctg ttttcggccg aggaactcga
     6661 cggcttcctg cggctgcgcg accagcgcgt gatcccggcc ggtcaccgct gcgtactgtt
     6721 cgtgctcggc cgctacagtg tcgactttca gtccgccccg gaagatctcg agccttttct
     6781 gcagcgcgat ctggccggcc tcgactggtt cacctgcgcc ttcgggcgcc gcgagcagga
     6841 ttgcgtactg gcggcggtcg aagccggcgg ccacgcccgc atcggcttcg agaacaatct
     6901 ctacctgccc gacggcgaga tcgcggccga caccgcggcg ctggtaacca gcctcgccgc
     6961 cgcgctgcgt gccacaggcc ggcgcccggc gagtgccgcg gccgctcgtc agctgctcgg
     7021 ggtgcgcagc gcgtaggcca gcaacgcggt cgccagcagg caaaacgccg ccagcagtat
     7081 cgagatcgcg gcgaacgcaa cgtcccattc gagcaacgtg ccgaccagcg ccggtccgag
     7141 cgccgaagcg aacaccatca ccgcattggt caatgatttg atcgcaccga ggtggcgggc
     7201 gccgtagagc tcggcccaca acgccgacag gccggtgaaa tacagcccgg tgctgagacc
     7261 catcagcagc atgtaaggcc agattatcca gtgctggtcg gacaggttga gcaccaccag
     7321 tgccaccgcc agcggcagca ggaacagcgg caccacgcga cgcgcgccga agcggtcgat
     7381 caacagcccc gaataaatag ttgttgccac cgacaccgtc gagtaaagcc agtagttgcc
     7441 ggtcacccac aggctgctcc agtgcttggc cgcggcaatc tcggcgggaa agaagaacag
     7501 cgcggtgccg atcatcgacg gcgatatcat cgccggcagc atcaggtaga aacgtgtctc
     7561 ggagagcatt tgtcggcgcg tgtagtcggc catgccgccg gtttccgcgg cccgccgctc
     7621 gagcgccgcc gaatgctcgg catggcgccg gccgtgcccc ttgagcaacc acagggccgg
     7681 caggatcgcg gccagcacgc agacggcaac cgcgtgaaac gtcattcgcc agccgatcgc
     7741 ctgcgcggca taaaggccgg ccagcggcag taccgcctcg ccacaggaaa agccgatcgc
     7801 cgccagtgcg atcgccttgc cgcggtcggc gtcatggtaa cgcgccatcg acgtgatgcc
     7861 ggtatgactg gtcagtccct ggccaaactg gcgcagcaaa aagatcgccg ccgccagcag
     7921 taacggcgag gtcacgctgc ccatcaccag gcaggcggcg gccagcccgg cgagtgtcgc
     7981 cacggtgaat attctcaggt cgaagcgatc gatcagcgag ccgctccagt tgatcaccag
     8041 cgccgaggcc agtgtaccaa tcatataggt gcgcccccag ctgcccgggt cgagaccgaa
     8101 gtcggcctga atcgcggtgc cgaaaacgcc gacgaaatac gtctgtcccg cgctcgacag
     8161 gaaagccagg aaaaaaccga atccgagaaa ccgcgggttc gcgcgcgcga acctccagta
     8221 atcgacgagc atcgcaccgc taaccggtaa tgacagaacc gcgccacttt acagggattc
     8281 gggcgccaac ggaatcgcat cgcgacgccg gcttcgccgg ctggtgacga tgggccgatc
     8341 ggcgtagtat tggcggcgac gccagaactc aacgcggtca atccgcgagc gaaaaataac
     8401 ggaggcgacc agcatggctg agattcatcc ccggctgatc gccgattgca taccgctggt
     8461 gcgcctgccg ctctgccacc tgctgctaat gaacgacagc aacttcccct ggttcattct
     8521 ggtgccggac cgcgagggta tacgcgaaat ctaccagctg gacgacgctg accgcgcgca
     8581 actgttgcgg gaatcctgcc agctcggcga attcctgatg cgccatttcg ccggcgacaa
     8641 gttgaacgtc gccgcgctcg gtaaccaggt
//
LOCUS       DATCZV010000603         6713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_154799, whole genome shotgun sequence.
ACCESSION   DATCZV010000603 DATCZV010000000
VERSION     DATCZV010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154799"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VIW27_13595"
     CDS             complement(<1..1584)
                     /locus_tag="VIW27_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740756.1"
                     /translation="MRRGEGLVVSVTPTGFGADLDSVELRLNGVVVGSKSAAPYEWSH
                     EDLGQLDQLEPGDYRLEALAADINGTQATDSVAFEVSQQPELSLAAYRPSQPDFSTPK
                     PVTYAHNTTAQRVPAVVIETPFQGNGHSVSWDGRLYLVTNTGGWYLRVLRPENITRNA
                     AGTPNFNNSAFSTRVCYEHDGDSTRCTSNLNGAFDHNWVAIVRNTAFDRNPYPSDVAG
                     NADDNGAYLTYEAFIYHTRVPAGGGKAVGFRSTRITLSQAYTPAAQVDSFVIRDDWQD
                     LTDTNGNFINCIEPSLTIDGRLMVCNGNRTGNGGINDIAYTWTGTTGSASGWRTLISL
                     ANLYNDRDQLIDGKTLGERFPLARRPIRDMDGNILDGTDEINGAYPWISRDGSEVFFQ
                     SMRAVDNANRAGTMVIGRWTGWSTRLLDNPINPDRLGTGKRRLFMSSPGAFTTMWSAF
                     TDIEDLPIPYSARGPVFPIFGSNTRDYSEIEFEDYLDGNYWVALDMNEKISRDWQFVT
                     NQTPDTSGLFNNATLINAQF"
     gene            complement(2096..2638)
                     /locus_tag="VIW27_13600"
     CDS             complement(2096..2638)
                     /locus_tag="VIW27_13600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5740757.1"
                     /translation="MFFPPSRPAGRFRLLFCGKVFPRKGGMAALHAWREASLDGAEFW
                     LLGRIAPDHQHRYREAATDSVRFLGYDPSPGDAMRQCHAQILLSSAEGQAKSLIEGAA
                     CGLVTIATAATGFSFERGDLGFAVDADDIDGIVEILRRLHAEHDFRRRLSANSANVMR
                     EHYQWASFRQRLVNAVEAVI"
     gene            complement(2719..3942)
                     /locus_tag="VIW27_13605"
     CDS             complement(2719..3942)
                     /locus_tag="VIW27_13605"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5740758.1"
                     /translation="MKAKSRVRFGMPTSVLIPESIGIERELRSNFRHVDGSPLDETDL
                     SQYDRYTLFYDVFFNIDNDRLYLLGPPLLNLGRQLLPLRARLNGKDIDLSIKEFFKQS
                     YFEVSGEVDPGSVGAINRVELDFNGVFDVALDIERNRQQRQTRILSTLQKNNRSRWIK
                     DWIRYYREYFGIDAVVLYDNGSENIEALRKELDGVIIEDWDYPYGIVRSHANKFCQQG
                     SLNHCRLKYGCGAEIFNFDIDELLCVDADWLDRQLQEFDVVSFDSYQVPLLDGGTNDY
                     SFGDFSKRNRDSRGGAKKYIYRDSAVVANKAHFVRTVENEYLNKVQRQLLRLFRKMKD
                     RGILTSLATFLAERISQTRQVRLSEGYFLHYVGITTNWKGAYYDRLQEETGRDDFVDI
                     DPACLRKIRNVCGPQ"
     gene            4579..5904
                     /locus_tag="VIW27_13610"
     CDS             4579..5904
                     /locus_tag="VIW27_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5740759.1"
                     /translation="MTIHAFRRLFFSIVLIISGTGLAASDSQLQHAQSLLDSGRLAEA
                     EVAYQNVLLDEPRSIEANLGLATINAWKGDYRLAIFRYGNVLEQQPDHFQALVGLGYA
                     QGWSGQFDKAQATFARAARLAPENFDVEKGAAFIALWRADAAAAVAGFERLVADHPDD
                     VELRVALGQAYLLDTNSQGAIESFNAALLREPDNNAARSGYLAAHSMPSKLEVDILWG
                     NNDGDTGLRQVEIGSWVNTRSRIWLRYDDSLSLDVPDLTSGDAETLLIGVFHQFDDGW
                     LGKFEFGQRDLPDGEGQDIYSFEVTRLLQRKNYKLGLQSAPHSNDYTDTLLYTSFGFA
                     SGEHWFIEPTLYLSETGQLEDQSRRLLTRFAYQSDSLWGIDFGIGFGETRSDLDEFDG
                     SIRTNSIDWYMPISPSVRISILFSNEETPASDTTSAILGVTWRMQKGEK"
     gene            5901..>6713
                     /locus_tag="VIW27_13615"
     CDS             5901..>6713
                     /locus_tag="VIW27_13615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5740760.1"
                     /translation="MKHHSNLKAQGNTLTAGPMPLQHFAYANRPWSQQQRFIRNIACR
                     SPVFYLTVFSLWALSVYWFHDRLFGLLESASGPVVTASLLYFVVFTQIAWLYGIYNLT
                     VVVFSSLLLRRREANIGTANARAVARSNTPVAVLYTTCNDFVLESAVSCLAMNYDNFK
                     IYILDDSSDPAYREKIDRFESLNRERVQVVRRANRRGFKAGNLNHALKHVVREPLFVI
                     ADADEIMPPDFLCKLVPRIEEDPNCGFIQANHKSKSWGISKLEQDMGIGIDIH"
BASE COUNT         1407 a         2013 c         1924 g         1369 t
ORIGIN      
        1 gaactgggca ttgatcagcg tcgcattgtt gaacaggccc gaggtgtcgg gcgtctgatt
       61 ggtcacgaac tgccagtcgc gggagatctt ttcgttcatg tcgagcgcaa cccagtagtt
      121 gccatcgaga taatcctcga attcgatttc gctgtagtca cgcgtattgg agccgaagat
      181 cgggaacacc ggaccgcggg ccgaatacgg aatgggcagg tcctcgatat cggtaaacgc
      241 cgaccacatg gtggtaaatg cgcctggcga cgacatgaac aggcgccgtt tgccggtgcc
      301 caggcgatcc gggttgatcg ggttgtcgag cagacgcgtc gaccagccgg tccagcgacc
      361 gataaccatg gtgccggcgc ggttggcatt gtccaccgcg cgcatgcttt gaaagaaaac
      421 ctcggagcca tccctgctga tccacgggta ggcgccgttg atttcgtcgg tgccgtcgag
      481 aatattgccg tccatgtcac ggatcgggcg gcgcgccagc ggaaatcgct cgcccagggt
      541 cttgccgtcg atgagctgat cgcggtcatt gtacagattg gccagtgaaa tcagtgttcg
      601 ccagccgctg gcgctgccgg tggtgccggt ccaggtgtaa gcgatgtcgt tgatgccgcc
      661 attgcccgtg cggttgccgt tgcagaccat cagccgcccg tcgatcgtca ggctgggttc
      721 gatgcagttg atgaaattgc cgttggtgtc ggtcaaatcc tgccagtcat cgcgaatcac
      781 gaatgaatcg acctgtgcgg ccggcgtgta ggcttggctc agggttattc gggtcgaacg
      841 aaatccgacc gccttgccgc cgcccgccgg tacgcgggta tggtagatga aggcttcgta
      901 ggtgaggtat gcgccgttgt cgtcggcatt gccggcgacg tccgacggat acgggttgcg
      961 gtcgaaggcg gtgttgcgca cgatcgctac ccagttgtga tcgaaggcgc cgttcaggtt
     1021 ggaggtacag cgggtggaat cgccgtcgtg ctcgtaacaa acccgagtcg aaaaggcgct
     1081 gttgttgaag ttcggcgtac cggcggcgtt gcgggtaatg ttttcggggc gaagcacgcg
     1141 caggtaccag cctccggtat tggtgaccag gtagagccga ccatcccagc tgacactgtg
     1201 gccgttgccc tgaaacggcg tctcgatgac taccgctggc acccgctgcg cggtcgtatt
     1261 gtgcgcataa gtcaccggct tcggcgttga aaagtcgggc tggctcgggc ggtatgccgc
     1321 caggctgagc tccggctgtt gcgatacctc gaaggcgaca ctgtcggtag cctgggtacc
     1381 attgatgtcg gccgccaacg cctcgagtcg ataatcgccc ggctccagtt gatcgagttg
     1441 gccgaggtcc tcgtgcgacc actcataggg ggccgccgac ttgctgccga cgacgacgcc
     1501 gttcagccgc aactcgacgc tgtcgaggtc ggcaccgaaa ccggtcggcg ttacgctgac
     1561 gaccagcccc tcgccgcgcc gcatcgccgt cgtatccgca cccgaggagg ttgaaaactc
     1621 gatcgccagc gaggcgtcgg caccgctcga accagtgctg cccgagtcgc cagcgccgcc
     1681 cgttccaccc gtattacccg aaatgcccga gtctccgctg tcggcgcatg cggccagcaa
     1741 aagaagaaaa caaataccaa aacccgtgtt gcgaattttc atcgaaggcc caagagcaaa
     1801 gctttgccac cattgagctt agtagccgcg agcggatcac cgagggccca gttatcatat
     1861 ttgatcgtcg gccgaccgac ggtcgtgtcc aggcgaccag cggctcgagg aaaagggcac
     1921 gtcaagccac gcgggtttcg ccattctcaa gtgccggcat tttggcggaa gcggatttgc
     1981 cgtgcgtgca gactgcccgg atgggaccta tagagggctg cagcagttac gcccgggatc
     2041 gggaactatc gaacaaagaa ccgctggctg acagcgcctc gaaggggcgc ccgggctaga
     2101 tgacagcctc cacggcgttg acgagccgtt gccggaaaga tgcccattgg tagtgctcgc
     2161 gcatgacatt ggccgaattg gcagacaggc gccgacgaaa gtcgtgttcc gcatgtaatc
     2221 gacgcaggat ctcgacgatg ccgtcgatat cgtcggcatc caccgcaaag cccagatccc
     2281 cccgctcgaa ggaaaaaccg gtagcggcgg tcgcgatcgt caccaggccg caggcggcac
     2341 cttcgatcaa cgatttggcc tgtccttcgg cggacgacag caggatttga gcatgacact
     2401 ggcgcatggc gtcaccggga ctggggtcgt agcccaaaaa ccgcacactg tcggttgcag
     2461 cttcgcggta cctgtgctgg tgatcggggg cgatcctgcc gagcagccag aattcggcac
     2521 catccaacga tgcctcgcgc cacgcatgca gcgctgccat gccccctttg cgcggaaata
     2581 ccttgccgca aaataatagt cgaaacctgc cggccggccg tgaaggcggg aaaaacatgg
     2641 caggatcgta acctcgctcg attacatgca gtggttgctc gggaacgccg ggcagatctc
     2701 gccgctccgc gaacgggttc actgcggccc gcagacgttc cgaatttttc gcaggcaggc
     2761 cggatcgatg tcgacgaaat cgtcgcgccc ggtttcctcc tgcaaccgat cgtaataagc
     2821 gcctttccag ttggtggtaa ttccaacgta gtgcaggaaa taaccctcac tcagcctgac
     2881 ctgccgagtt tgggaaatcc tctcggccag aaaagtcgcc aggctcgtca gtatccctct
     2941 gtccttcatc tttctgaaca gccgcaaaag ctgccgttga actttgttca ggtactcgtt
     3001 ttcgacggtt ctgacaaaat gcgccttgtt ggcaaccacc gccgagtcgc ggtagatata
     3061 ctttttagcg cctcccctgc tgtctcgatt gcgttttgaa aaatcgccaa aggaatagtc
     3121 attcgtacca ccgtcgagca aaggcacctg gtaactgtcg aacgatacca cgtcaaattc
     3181 ttgcagctgg cgatccagcc agtcggcatc cacgcacagc agttcatcga tgtcgaaatt
     3241 gaagatctcg gccccgcaac catatttcag gcgacagtgg ttcagggacc cctgctggca
     3301 aaacttgttg gcatgactcc tgactatgcc atacgggtaa tcccagtctt caataatcac
     3361 cccgtccagc tctttccgta aagcctcgat attctcgctg ccgttgtcat acaacacgac
     3421 tgcatcgata ccgaaatact cgcggtaata gcgaatccag tcctttatcc atctggaccg
     3481 gttgttcttc tgcagggtgg acaagatacg cgtttgccgc tgctgccgat ttcgttcgat
     3541 gtccagcgcg acatcgaaca caccattgaa atccagttcg accctgttga tcgcgccgac
     3601 agacccggga tcgacttcgc cgctgacctc aaagtagctt tgcttgaaga attcctttat
     3661 cgataaatcg atatccttgc cgttcagtct tgccctcagg ggcagcaact gccgccccag
     3721 gttcagcaaa ggcgggccca gtaaatacag gcgatcattg tcgatgttga agaatacatc
     3781 gtaaaacagg gtgtacctgt cgtattgaga caagtcggtc tcgtccagcg gactgccgtc
     3841 cacgtggcgg aaattcgaac gcagttctct ttctatcccg atcgattccg ggatcaatac
     3901 acttgttggc atgccgaacc tgacccgaga tttcgccttc aaccccaacc gatcgggttg
     3961 ctgatcggag tcgacgaagt tgctttgtag gctgcgagaa aaatgcagaa atgctgactt
     4021 ttcggcgcca tgttaacaac caccaatgcg agcatcaata tcaccgattt ccggcccggt
     4081 ctcggcttga cattcatcgg ccctgaatgc tcgcacgagt aaaaacggac tgttgccgaa
     4141 tcgagcggct agcggtggac gcaaaatagc cgccattctt atgcatcgcc agatcgcttc
     4201 cgggccgaat tcgacaaacg ggccttacct ggggtaattc gctggctgcg cggcaagtga
     4261 atgcgggcct ccgtctccct gtcgacggag aggcccgaca cttcgaggca tttgccgagc
     4321 tgcccggccg ccttcgtctt gcggtcgatt cgcgaccatg agtcctggcg gcaaacaccg
     4381 cccgacgcgg taatactgcc gcgaatgcga gctccgcatc accgccgcac ttatccaccc
     4441 gttagaaccg aacgcacgac aaatgcgcaa aaagccaatt ttttcgcccc agagtattgc
     4501 gtttaattat attttgggat ttaattccca aaatataatt aaatatggga attaattccc
     4561 acaaagcgag cgacgaatat gaccatacat gccttcagaa ggctgttttt cagcatagtt
     4621 ttaatcattt caggcaccgg actggcagcc tcggactcgc aactacagca tgcgcagagc
     4681 ttgctggaca gtggccgact ggccgaggcc gaggtcgcct accagaatgt cctgctcgac
     4741 gaaccccgca gtatcgaggc caatctcggg ctggcgacga tcaatgcctg gaaaggcgat
     4801 tatcgtctgg cgatatttcg ctacggcaac gtgctcgaac agcaacccga ccatttccag
     4861 gcactcgtcg gtctcggtta cgcgcagggc tggtccggtc agttcgacaa ggcgcaggct
     4921 accttcgccc gggcggcacg tctcgcgccc gaaaatttcg acgtcgaaaa gggtgcggct
     4981 ttcattgcct tgtggcgcgc ggatgccgca gcagccgtcg ccggcttcga acggctcgtc
     5041 gccgaccacc ccgacgatgt cgagctgcgc gtcgcgctcg gccaggccta cctgctggat
     5101 acgaacagtc agggcgcgat cgaaagtttt aacgccgcgc tgctgcggga gcccgacaac
     5161 aatgccgctc gcagcggtta cctggcggcg cattcgatgc cgtcgaaact ggaagtcgac
     5221 atcctctggg gtaataacga cggcgatacc ggcctgcgcc aggtcgaaat cggctcctgg
     5281 gtaaatacgc gctcgcgaat atggctgcgt tacgacgaca gcctctcgct ggacgttccc
     5341 gacctgacca gcggcgatgc ggaaaccctg ttgatcggcg tgtttcacca gttcgacgac
     5401 ggctggctcg gcaagttcga attcggtcag cgcgatctgc ccgacggcga gggccaggat
     5461 atctacagct tcgaagtcac ccggctgctg cagcggaaaa actacaagct cgggctgcag
     5521 tcggcacctc acagcaacga ctacacggac acattgctct acaccagctt tggctttgcc
     5581 agcggcgagc actggtttat cgaaccgacc ctgtatctgt ccgagaccgg acaactcgaa
     5641 gaccagagcc ggcgcctgct gacccgattc gcctatcaga gcgatagcct gtggggcatc
     5701 gacttcggca tcggtttcgg cgaaacccgc agcgacctgg atgaattcga cggcagcatc
     5761 cgcaccaaca gtatcgactg gtacatgccg atatcaccgt ctgtgcggat atcgatcctg
     5821 ttcagcaacg aagaaacgcc ggcgagcgac accaccagcg ccatcctggg agtcacctgg
     5881 cgcatgcaaa agggggaaaa atgaaacacc acagcaacct caaggcgcag ggaaacacgc
     5941 tgactgccgg ccccatgccg ctgcagcatt tcgcctatgc caatcgtcca tggtcgcagc
     6001 aacagcgctt cattcgcaac attgcctgcc gcagcccggt attttacctg acggtgttca
     6061 gcctctgggc attgtcggtc tactggttcc acgaccgcct gttcggcttg ctggaatcgg
     6121 ccagcgggcc cgtcgtcacc gcgtcgctgt tgtacttcgt cgtcttcacc cagatcgcgt
     6181 ggctttacgg catttacaac ctcaccgtgg tcgtcttttc cagcctgttg ctgcggcggc
     6241 gggaggcaaa tatcggcact gcaaatgcgc gcgcggtggc gcgcagcaat accccggtcg
     6301 ccgtgctcta caccacctgc aacgacttcg tgctggagag cgccgtttcc tgcctggcga
     6361 tgaactacga caatttcaag atctacatac tcgacgacag ttccgacccg gcgtaccgtg
     6421 aaaagatcga ccgcttcgag tcgctcaaca gggaacgggt tcaggtcgtg cgccgtgcaa
     6481 accggcgcgg cttcaaggct ggcaatctca accacgccct caagcacgtg gtgcgcgagc
     6541 cgttgttcgt gattgccgat gccgacgaaa tcatgccgcc cgatttcctg tgcaagctgg
     6601 taccgaggat cgaggaagac cccaattgcg gtttcatcca ggccaaccac aagagtaaat
     6661 cctggggtat ctcgaagctc gaacaggaca tggggatcgg gatcgacatc cac
//
LOCUS       DATCZV010000604         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_419394, whole genome shotgun sequence.
ACCESSION   DATCZV010000604 DATCZV010000000
VERSION     DATCZV010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419394"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VIW27_13620"
     CDS             <1..755
                     /locus_tag="VIW27_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5740761.1"
                     /translation="MRSTLTRQRLAETAAREALIRQLGLWGNAARKLTLPSRLPALPA
                     SPLDYPSVEGEAIAGRLDVQIAKATIEATASNYSLTRRSPFLSSIEFGPVAESGGDGT
                     EYGYELEFRIPIFDAGGIANEKARFLFEQSQDRARSIAISAASEAREAYQTYHSHWDI
                     ARYYEKVMLPLRQQVSEERLLHYNGMLISVFDLLDDVRGAADLEASAIRAARDFWLAD
                     ARLQLALTGSSPGMAMNTSVEMPAAGGADADH"
     gene            768..2048
                     /locus_tag="VIW27_13625"
     CDS             768..2048
                     /locus_tag="VIW27_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEY5740762.1"
                     /translation="MTDRRNFLKGMGAAAAIGAGLVSRSALAALPESPTSNATTLEVP
                     PEPATGPWYQPVVTLNSWSLPWRMKNGWKEFHLTAEAVERELAPGMTARLWGYNGQSP
                     GPTIECVEGDKIRIFVTNRLPEHTSIHWHGIFLPAGMDGVGGLNQPQIPPGKTFAYEF
                     ELKQSGTFMYHPHADEMVQMAMGMMGSLVVHPKDPNLHRVDRDYCFLMAAYDIEPGTM
                     TARINTMLDFNLWTWNSRVFPGIDHLVAGLNERVRVRMGNLTMTNHPIHIHGLKFEVA
                     CTDGGWVPKQARWPEVTVDIAIGQMRAVEFIADAPGDWAMHCHKSHHTMNPMGHDVPN
                     MIGVDQSRVQRSLGKLLPGYMIMGERGMADMGEMVMPLPRNTLPMMTGQGQFGPIEMG
                     GMFTVLKVRENQTRGDYSDPGWYDYPPGTVAYEV"
BASE COUNT          421 a          690 c          656 g          342 t
ORIGIN      
        1 aaatgcgctc tacgctgacc cggcaacggc tggcagaaac cgcggcgcgc gaagccctga
       61 ttcgtcagct cggcctgtgg ggcaacgccg cgcgcaaact gactctgccg tcgcgcctgc
      121 cggcgctgcc ggcctcgccg ctggactacc cctcggtcga gggcgaggcg atcgcgggaa
      181 ggctcgatgt acagatcgcg aaggcgacga tcgaggccac ggccagcaac tactcgctga
      241 cacgacgcag cccgttcctg tcgtcgatcg agttcggccc ggtggcggag agcggtgggg
      301 acggtaccga gtacggttac gagctcgaat tcaggatccc gatattcgac gcgggcggca
      361 tcgccaacga aaaggccagg tttctgttcg agcagtcgca ggaccgggcc cgttcgatcg
      421 cgatctcggc cgcctccgag gcgcgcgaag cttaccagac ctaccacagt cactgggaca
      481 tcgcgcgcta ctacgaaaag gtcatgctgc cgctgcgaca acaggtcagc gaggaacggc
      541 tgctgcacta caacggcatg ctgatcagcg tcttcgacct gctcgacgac gtgcgcggcg
      601 ccgccgatct cgaggccagc gcgattcgcg cggcgcgcga cttctggctc gccgacgcgc
      661 ggctgcagct ggccctgacc ggctcgtctc ccggcatggc aatgaacacg agtgtcgaaa
      721 tgcctgccgc gggcggtgcc gacgcggatc actagaggat taccgtaatg actgacagaa
      781 gaaattttct caagggcatg ggcgccgcgg ccgcgatcgg cgccggcctg gtatcgcgtt
      841 ccgcgctggc cgcgctgccg gaatcgccga cttccaacgc caccacttta gaggtgccac
      901 ccgaaccggc caccgggccc tggtaccagc cggtggtaac gctgaacagc tggtcgctgc
      961 cgtggcgcat gaaaaacggc tggaaagaat ttcacctcac cgccgaggcg gtagagcgcg
     1021 aactagcgcc cggcatgacc gcccgcctat ggggttacaa cggccagtcg ccgggaccga
     1081 ccatcgagtg cgtcgagggc gacaagatca ggattttcgt gaccaatcgc ttgcccgagc
     1141 acacctcgat ccactggcac ggcatcttcc tgcccgccgg catggacggc gtgggcggtc
     1201 tcaaccagcc gcagattccg cccggcaaga ccttcgccta cgagttcgag ctgaagcagt
     1261 ccggcacctt catgtaccac ccgcacgccg acgaaatggt gcagatggcg atgggcatga
     1321 tgggctcgct ggtggtgcat cctaaggatc cgaacctgca tcgggtcgac cgcgactact
     1381 gcttcctgat ggcggcctac gacatcgaac cgggcacgat gaccgcacgc atcaacacca
     1441 tgctcgattt caacctgtgg acctggaaca gccgcgtttt tccgggcatc gatcacctcg
     1501 tcgccggtct caacgagcgc gtgcgcgtgc gcatgggcaa cctgactatg accaaccacc
     1561 cgatccacat acacggcctc aagttcgagg tcgcctgtac cgatggcggc tgggtgccga
     1621 aacaggcgcg ctggcccgag gttacggtgg atatcgccat cggccagatg cgcgcggtcg
     1681 aattcatcgc cgacgcccca ggcgactggg cgatgcattg ccataagtcg caccacacga
     1741 tgaacccgat gggccacgac gtgcccaata tgatcggcgt cgaccagagc cgcgtgcagc
     1801 gcagcctcgg caagctgctg cccggttaca tgatcatggg cgaacgcggc atggccgaca
     1861 tgggtgaaat ggtcatgccg ctgccgcgca acacgctgcc gatgatgacc ggccagggcc
     1921 agttcggccc gatcgaaatg ggcggcatgt tcaccgtgct caaggttcgc gagaaccaga
     1981 cgcgcgggga ctacagcgat ccgggctggt acgactaccc gcccggcacc gtcgcctacg
     2041 aagtctgatt aatacttatc gataacaaaa cacgggagtt gaataatgaa cagtaaacca
     2101 tccttatcc
//
LOCUS       DATCZV010000605         3507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_737639, whole genome shotgun sequence.
ACCESSION   DATCZV010000605 DATCZV010000000
VERSION     DATCZV010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737639"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VIW27_13630"
     CDS             <1..310
                     /locus_tag="VIW27_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740763.1"
                     /translation="LILEWQWLTGELGFALIFLVLNVMHGGVRLGRKVYLVDMASAET
                     RAAYVAVSNTTIGVLMLFGGLIGLVGDWLGAASILLLLGLLSLVAACGALRLPEVSEP
                     "
     gene            389..1411
                     /locus_tag="VIW27_13635"
     CDS             389..1411
                     /locus_tag="VIW27_13635"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEY5740764.1"
                     /translation="MLPAAEDLDRHPVAEQVSAVGEVPVIDVGAVFADSSGNDAVRAI
                     EQIAEACRSWGFFQVVNHGIGAPQIDQVWRQTRALFALPLADKLAILRDRQSPWGFYN
                     NELTKNQRDKKEVFDFTREGIDPIYGQQNRWPAQPTEFRPTMLAYFEACTELSLNLLE
                     AFCRGLDLPAKFMHKDFDGNHTGFVRLNYYPVEDPMAELGGEQSAAELGVHHHTDAGA
                     LTVLLQDEVSGLQVYRDGLWYDIPPVGGAMVINTGDMMQVWSNDIYRAAIHRVLAMDA
                     RDRYSIPFFFNPAADCTVSPLPSVIGDRRPSRYRPIEWGSFRGQRSLGDYADYGTEVQ
                     ISQYRI"
     gene            1432..1923
                     /gene="greB"
                     /locus_tag="VIW27_13640"
     CDS             1432..1923
                     /gene="greB"
                     /locus_tag="VIW27_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY5740765.1"
                     /translation="MSRYRPPAPPKSPYITPDGMRALEDEQKAIWPRRREVVAALSAA
                     AAEGDRSENAEYIYRKKELREIDRRIRYLQKRLPDLRVVERKPSDPDTVYFGATVKLE
                     DADGDTVSYRIVGPDEFDRAEHYISVDSPLARSLLKRELGDEIEVDVPSGRLAYMIVG
                     IDY"
     gene            2139..>3507
                     /locus_tag="VIW27_13645"
     CDS             2139..>3507
                     /locus_tag="VIW27_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5740766.1"
                     /translation="MAIAPAEAYINRNYSSLEQTEVFTGDFNDILRQGKLRILLTRDF
                     SSASYLPRRRSPLAEQQRIAEEFALSHGLIPELVIVDNFSKLIPALLEGRGDVIVDNL
                     TITDIRLQKMAFSVPVDHVREKVIVASGNDSIERVRDLDGKKVMVNKGSTFWQALKWL
                     QENRYPNIEILETADGVTIEEVLDQLADGEFDATIMDSNLVEIYQAYRDDFKIAVNFS
                     AQRDIGWGVRKNATQLVSEINRYLQLEYRAEGRDARFTGDLDEIEERRVLRVLLRNNA
                     ASYFVYRGELMGFEYELAREFADYHGLRLELVVPPSHRELTTWLLEGRADIALSFLEP
                     NARQRKLGVAYSQPYHFARQHIVVQKQDPANSLEDLDYRTIFVRHHSTYWERLSELQR
                     EGARFSLRATEDDIETEQLIGMVSRGKYKATMADEQLLDIELAKGVPVRSAVVLESER
                     AHAVAL"
BASE COUNT          650 a         1099 c         1119 g          639 t
ORIGIN      
        1 gctgatactc gaatggcagt ggttgaccgg cgaactcggg ttcgcgctga ttttcctggt
       61 gctcaacgtc atgcacggcg gtgtgcgcct cgggcgcaag gtgtacctgg tcgatatggc
      121 cagcgccgaa acccgcgccg cctacgtcgc ggtatcgaac accacgatcg gcgtgctgat
      181 gctgttcggc ggcctgatcg ggctggtcgg cgactggctc ggtgcagcgt cgatactgtt
      241 gctgcttggc ttgttgtcgc tggtcgcggc ctgcggcgca ctgcgcctgc ccgaggtcag
      301 cgagccctga gcggcgcgcg ccgccatttg ccaacaccgg tgtaagtcat atactgaccc
      361 atccacggac cacacgagat acgcgcgcat gctgcccgct gcagaagacc tcgaccgcca
      421 cccggtcgcc gagcaagtct cggccgtcgg cgaggtgccg gtgatcgatg tcggcgcggt
      481 gtttgccgac tcttccggca atgacgcggt gcgcgccatc gagcaaattg ccgaggcttg
      541 ccgcagctgg ggcttctttc aggtcgtcaa ccacggcatt ggcgcgccgc agatcgacca
      601 ggtctggcga cagacccgcg cgctattcgc gctgccgctg gcagacaagc tggcgatcct
      661 gcgcgaccgc caaagcccgt gggggtttta taacaacgag ttgaccaaga accagcgcga
      721 caagaaggaa gtcttcgatt tcacccgtga gggcatcgat ccgatctacg gccagcaaaa
      781 ccgctggccc gcgcaaccga ccgagttccg gccgacgatg ctggcttatt tcgaggcctg
      841 caccgagttg tcgctgaacc tgctggaggc gttttgccgt ggcctcgacc tgccggcgaa
      901 gttcatgcat aaggatttcg acggcaacca taccggtttc gtgcgcctca attattaccc
      961 ggtggaagac ccgatggcgg agctcggcgg cgagcagtcg gccgccgagc tcggcgtgca
     1021 ccaccatacc gacgccggtg cgctgaccgt gctgctgcag gacgaggtta gcgggctgca
     1081 ggtttaccgc gacggtttgt ggtacgacat tccgcccgtc ggcggcgcga tggtgatcaa
     1141 taccggtgac atgatgcagg tgtggtcgaa cgatatctat cgggcggcaa tccatcgcgt
     1201 gctggcgatg gatgcgcgcg accgttacag cattccgttt ttcttcaacc cggcggccga
     1261 ctgcacggtc agcccgctgc cttcggtgat cggcgaccgg cggccgagcc gctaccggcc
     1321 gatcgagtgg ggcagctttc gcggccagcg ctctttgggt gattacgcag attacggcac
     1381 cgaggtgcaa atctcgcaat accgaatctg acgcccctgc gtcgtccgcg catgagccgt
     1441 taccgtcccc ccgccccgcc gaaatcgcct tacatcacgc ccgacggcat gcgcgcgctg
     1501 gaggacgagc aaaaggcgat ctggccgcgt cggcgcgagg tcgtagccgc gctgtcggcg
     1561 gcggcggccg agggcgaccg ctcggaaaac gccgagtaca tttaccgcaa gaaggaattg
     1621 cgcgagatcg accggcgtat tcgctacctg caaaaacgac tgcccgacct cagggtggtc
     1681 gagcgcaaac ccagcgatcc ggataccgtg tatttcggcg ccacggtgaa actcgaggat
     1741 gccgacggcg acacggttag ttaccgcatc gtcggccccg acgaattcga ccgcgcggag
     1801 cactatatca gcgtcgattc accgctcgct cgctcgctgc tgaaacgcga actcggcgac
     1861 gaaatcgagg tcgatgtgcc cagcggccgg ctggcctata tgatcgtcgg aatcgactac
     1921 tgagccgtag tcgcgggccg gctgcgtgct tcggtcggtc gccggaaatt gtcccgcggg
     1981 ctcgacgatt acccctgaaa ccccgcgctg tggcggtttt ttgacgtttt taagttttga
     2041 catggccgcg aatacgcgct taagtagcgc ccatggggga gtcaaagttc agatttgggc
     2101 tagcgtccgc acgcctctgc ctgctggggc tggcggcgat ggcgatcgcc ccggccgagg
     2161 cctatatcaa ccgcaactac agttcgctcg agcagaccga ggttttcacc ggcgatttca
     2221 acgatatcct gcgccagggc aagctgcgca ttttgctgac ccgtgatttc tcgagcgcca
     2281 gctacctgcc gcggcggcgc tcgccgctcg ccgagcagca gcgcatcgcc gaggaattcg
     2341 cgctgtcgca cggcctgatc ccggaactgg tcatcgtcga caacttctcc aagctgatcc
     2401 cggcactgct cgagggccgt ggcgacgtga tcgtcgacaa tctcacgatc actgatattc
     2461 gcctgcagaa gatggcattt tcggtgccgg tggatcacgt ccgcgaaaag gtcatcgtcg
     2521 ccagcggcaa cgattcgatc gagcgcgtgc gcgatctgga tggcaagaaa gtcatggtca
     2581 acaagggctc gactttctgg caggcactga agtggctgca ggagaaccgc tacccgaaca
     2641 tcgagatcct cgagaccgcg gatggcgtca ccatcgaaga agtgctcgac cagctggcgg
     2701 acggtgaatt tgacgccacc atcatggaca gcaacctggt agagatctac caggcttacc
     2761 gcgacgactt caagatcgcg gtcaacttca gcgcccagcg tgatattggc tggggggtgc
     2821 gcaagaacgc gacgcagctg gtcagcgaga tcaatcgcta cctgcagctc gagtaccgcg
     2881 ccgagggccg cgacgcccgc ttcaccggcg atctcgacga gatcgaggag cgacgcgtac
     2941 tgcgggtact gctgcgcaac aacgcggcat cgtatttcgt ctaccgcggc gagctgatgg
     3001 gattcgagta cgagctggcg cgcgaattcg ccgactacca tggactgcgg ctcgagttgg
     3061 tggtgccgcc gagccaccgc gagctgacca cctggctgct cgagggccgc gcggatatcg
     3121 cgctgagctt tctcgagccg aatgcgaggc agcgcaagct cggcgtggcc tactcgcagc
     3181 cctaccactt cgccaggcag cacattgtgg tgcaaaaaca ggacccggcg aattcgctcg
     3241 aggacctcga ttaccgcacc atcttcgtcc gccaccacag cacctactgg gaacggctgt
     3301 cggaattgca gcgtgagggc gcgcgcttca gcctgcgcgc caccgaggat gatatcgaaa
     3361 ccgagcaact gatcggcatg gtgtcgcgcg gcaagtacaa agcgaccatg gccgacgagc
     3421 agctgctcga catcgaactc gccaaggggg tgccggtgcg ctcggcggtg gtgctggaaa
     3481 gcgagcgtgc gcacgctgtc gcgttgg
//
LOCUS       DATCZV010000606         3879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_791339, whole genome shotgun sequence.
ACCESSION   DATCZV010000606 DATCZV010000000
VERSION     DATCZV010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791339"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VIW27_13650"
     CDS             <1..981
                     /locus_tag="VIW27_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017007883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5740767.1"
                     /translation="EAGVPTVPGSDGPLDDNEQRNLEFGRKIGYPVIIKAAGGGGGRG
                     MRVVHNERNLLNAIQLTRSEAAAAFNNDSVYMEKYLGAPRHIEIQVLADAHGSAIHLG
                     ERDCSMQRRHQKVVEEAPAPGITAEQRDTIGRRCAEACVKMGYEGAGTFEFLFEDGEF
                     YFIEMNTRVQVEHPVTEMITWVDIIKEQISIASGLPLRFKQEDILIHGHAIECRINAE
                     DPKNFLPSPGKITLYHAPGGPGVRMDSHIYNGYTVPPYYDSMIGKLITFGNTRESAIA
                     RMSGALSEIVIEGITTNIDLQTEIIADRKFVAGGTDIHYLEKKLGLYKRE"
     gene            978..1880
                     /gene="prmA"
                     /locus_tag="VIW27_13655"
     CDS             978..1880
                     /gene="prmA"
                     /locus_tag="VIW27_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY5740768.1"
                     /translation="MSWWQFSLDCPASDLESVEDLMQELGAVSISIRDAVDDQPIYEP
                     PPGSQPVWSSSVLTATFDAYCDPDALQQRIADGLPADLAASLHRGSLQEQDWEQAYRQ
                     HFQPLECAPGLWIVPSWLEPPEPQATVIRLDPGLAFGTGSHPTTALCLAWLAARDLTG
                     LEVIDYGCGSGILAIAAALRGAGRVTAVDIDAQAITACEANLERNGITTDRVLVGQVE
                     TTPLAAADLLIANILAGPLVALAPCFAELLRPGGEILLSGILHSQLDAIISAYTPCFE
                     LEPATRREDWVCISGRKIERPENA"
     gene            1873..2655
                     /locus_tag="VIW27_13660"
     CDS             1873..2655
                     /locus_tag="VIW27_13660"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEY5740769.1"
                     /translation="MRKPTQLGEVMETTCSSCGSRFRLTEQQLRQALGKVRCGECGSQ
                     FNALHTLKSYEGELPPDFLRHLRAEPGAAASADHNAADAGGQARELSLHEAMYGSERR
                     SLFAIGPFGWFVGILLLGAIGIAQAVYYQRYQLIENPRYQQQVVNLCRVLPCAETEFA
                     SPEQIRMLERNVFTHPVANNALMVTGAFSNQAPFTQQLPDMLISLFDSQGKLIANRLF
                     APSEYLLEDRDRRVFEPGAKVQFRLEIVDPGTDALTYEFEFY"
     gene            2794..>3879
                     /locus_tag="VIW27_13665"
     CDS             2794..>3879
                     /locus_tag="VIW27_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5740770.1"
                     /translation="MNFQYHLNRYLPFLRWGRNANRESLRADAIAGLTGAAIVLPQGV
                     AFAAIAGMPPEYGLYTSMVPAIIAALFGSSWHLVSGPTTAASVIMFASLSGIAAPGSA
                     EYVSLAITLAFMVGVLQLIMGVVRLGALVNFISHSVVVGFTAGAACLIAAKQAREFLG
                     IDIPEGAHLFDTLEFLWVNWKDLHPLVTLVAVITLLAGILAQRRLGGMSMIVAMIVGS
                     IAAALFNVAFGEPTSGIHMVGALPQQLPPLSMPEFSLAVFRELSPLALAMTLFALTEA
                     VSIARSISLKSGQPIDGNQEFIGQGLSNIFGSFFSSYVATGSFNRSGANYDAGARTPL
                     AAMLAGVLLVVIVLLVAPLTSFLPQAAV"
BASE COUNT          696 a         1282 c         1198 g          703 t
ORIGIN      
        1 gaggccggcg tgccgaccgt gcccggctct gacggaccgc tcgacgacaa cgagcagcgc
       61 aacctcgaat tcgggcgcaa gatcggctac ccggtgatca tcaaggcggc cggcggcggc
      121 ggcggtcgcg gcatgcgcgt ggtgcacaac gaacgcaacc tgctgaatgc gattcaactg
      181 acgcgctccg aggcggcagc agccttcaac aacgactcgg tctacatgga aaagtacctg
      241 ggcgcgccgc gtcatatcga gattcaggtg ctcgccgatg cgcacggtag cgcgattcac
      301 ctcggcgagc gcgactgctc gatgcagcga cgacaccaga aggtcgtcga ggaagcgccc
      361 gcccccggca tcaccgccga gcagcgcgac accatcggcc ggcgctgcgc cgaggcctgc
      421 gtcaagatgg gctacgaggg cgccggcacc ttcgagttcc tgttcgagga cggcgaattc
      481 tacttcatcg agatgaacac tcgcgtgcag gtggagcatc cggttaccga gatgattacc
      541 tgggtggata tcatcaagga acagatctcg atcgcgtccg ggctgccgtt gcgcttcaag
      601 caggaagaca tcctgattca tggccacgcg atcgaatgcc gcatcaatgc cgaggatccg
      661 aaaaatttcc tgccgtcgcc cggcaagatc acgctatacc atgcacccgg tggtcccggg
      721 gtgcgcatgg actcccatat ttacaacggt tacacggtgc cgccctacta cgattcgatg
      781 atcggcaagc tgatcacttt cggcaatacg cgggaatcgg cgatcgcgcg catgagcggc
      841 gctctgtcgg agatcgtcat cgagggaatc acgaccaaca tcgacctgca aacggaaatt
      901 atcgccgacc gcaagttcgt cgccggcggt accgatatcc actacctcga aaagaagctc
      961 ggactctaca agcgcgaatg agctggtggc agttcagtct cgattgcccg gccagcgacc
     1021 tcgaatccgt cgaagacctg atgcaggagc tcggcgccgt cagcatctcg attcgcgatg
     1081 cggtcgacga ccaaccgatt tacgaaccgc cacccggtag ccagccggtc tggtcatcgt
     1141 cggtgctgac cgcgaccttc gacgcatact gcgaccccga cgccctgcaa cagcgcattg
     1201 ccgacggcct gccggcggac ctggcggcga gcctgcaccg cggcagcctg caggagcagg
     1261 actgggaaca ggcctaccgg cagcattttc agccccttga gtgtgcgccc ggcctgtgga
     1321 tcgtgcccag ctggctcgag ccgcccgagc cgcaggcgac ggtcattcgg ctcgatccgg
     1381 gactcgcctt cggcaccggc agccacccaa ccacggcgct gtgcctggcg tggctggcgg
     1441 cccgcgatct cactggcctc gaggtgatcg actatggctg cggctccggt attctggcga
     1501 tcgccgccgc gctgcgcggc gccggccggg tcaccgcggt cgatatcgac gcgcaggcga
     1561 tcaccgcctg cgaggcgaac ctggaacgca acgggatcac cacggatcga gtgctcgtcg
     1621 gccaggtgga aaccacgcca ctggccgccg ccgatctgct gatcgccaac atactcgccg
     1681 ggccattggt cgcactcgcg ccgtgctttg cagaactgct caggccgggt ggcgaaatat
     1741 tgctgtcggg aattctgcat tcgcagcttg acgctataat aagcgcatac acgccctgct
     1801 tcgaactcga acccgcgacg cgacgcgagg actgggtttg catcagcggc aggaaaatcg
     1861 agcggccgga aaatgcgtaa gccaacccaa ctcggtgaag tcatggagac cacctgcagc
     1921 agctgcggca gccgtttccg gctgaccgag cagcagctgc gccaggctct cggcaaggtg
     1981 cgctgcggcg aatgcggcag ccaattcaat gcgctgcata ccctgaagag ctacgaaggc
     2041 gaactgccgc ccgatttcct gcggcacttg cgcgccgaac caggcgccgc cgcctcggcc
     2101 gatcacaacg cggcagacgc cggcggccag gctcgcgaac tcagcctgca tgaagccatg
     2161 tacggctcgg aacgccgctc gttgtttgcc atcggcccgt tcggctggtt cgtcggcatc
     2221 ctgctgctcg gtgcgatcgg catcgcgcag gccgtctatt accagcgcta ccagctgatc
     2281 gagaatccgc gctatcagca gcaggtcgtg aacctgtgcc gcgtactgcc ctgtgccgag
     2341 accgagttcg cctcccccga gcagattcgc atgctcgagc gcaacgtctt tacccacccg
     2401 gtcgccaata acgcactgat ggtgaccggt gcgttcagca accaggcccc gtttacgcaa
     2461 cagctgcccg acatgctgat cagcctgttc gacagccagg gcaagctgat cgccaaccgc
     2521 ctgttcgcgc cttccgagta cctgctcgaa gaccgcgacc ggcgcgtgtt cgagcccggg
     2581 gccaaggtcc agttccggct cgaaatcgtc gatccgggaa ccgacgcgct gacttacgaa
     2641 ttcgaattct actgaacgtc gagtgcccat aagcgcaacc aatacccgga tcgaaaactg
     2701 ccgctactcg aaggcgcttg aacgggctat attgagtcat atgccgtcga ttcgcgcgca
     2761 caccgcgctc ggaccggtaa cccaatcacg ccgatgaatt ttcagtacca cttgaaccgt
     2821 tatctgccgt tcctgcgctg gggaaggaac gcgaatcgcg aaagcctgcg cgccgacgcg
     2881 atcgccgggc tcaccggcgc cgcgatcgtg ctgccgcaag gcgtcgcctt tgccgccatc
     2941 gccggcatgc cgcccgagta cgggctttat accagcatgg tgccggcgat catcgccgcg
     3001 ctgttcggtt catcctggca cctggtctcg ggaccgacca ccgcggcgtc ggtaatcatg
     3061 ttcgcgtccc tgtcgggcat cgcggccccg ggtagcgcag agtacgtctc gctggcgatt
     3121 acgctggcct tcatggtcgg cgtgctgcaa ctgatcatgg gcgtggtgcg gctaggcgcg
     3181 ctggtcaatt tcatctcgca ctcggtagtg gtcgggttta ccgccggcgc cgcctgcctg
     3241 atcgctgcca agcaggcgcg ggaatttctc ggcatcgata ttcccgaggg tgcgcacctg
     3301 ttcgatacgc tggagttcct gtgggtcaac tggaaagacc tgcacccgct ggtgacgctg
     3361 gtggcggtca tcaccttgct ggccggtatt ctcgcgcagc gccgcctcgg cggcatgagc
     3421 atgatcgtcg cgatgatcgt cggtagcatc gccgcggcgc tattcaatgt cgccttcggc
     3481 gaaccgacct cgggcattca catggtcggc gcgctgcctc agcagctgcc gccgctgtcg
     3541 atgccggagt tttcgctggc ggtgtttcgc gagctgtcgc cgctggcgct ggcgatgacg
     3601 ctgttcgcac tgaccgaggc ggtctccatc gcgcgctcca tttcgttgaa atcgggtcag
     3661 ccgatcgacg gcaaccagga gttcatcggc cagggcctgt cgaatatttt cggcagtttc
     3721 ttttcttcct acgtggccac cggttcgttc aaccgcagcg gcgccaacta cgacgccggc
     3781 gcgcgcacgc cgctggcggc gatgctcgcc ggggtgctgc tggtggtgat cgtattgctg
     3841 gtcgcgccgc tgacttcctt cctgccgcag gcggcggtc
//
LOCUS       DATCZV010000607         3835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_366428, whole genome shotgun sequence.
ACCESSION   DATCZV010000607 DATCZV010000000
VERSION     DATCZV010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366428"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VIW27_13670"
     CDS             complement(<1..369)
                     /locus_tag="VIW27_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY5740771.1"
                     /translation="MNLDNLGFDTLAVRSGQLRTAELEHSEAIFATSSFVFENAAQAA
                     ARFAGEQPGNIYSRFTNPTTRAFEQRLAALEGGEACVATASGMAAITSVCLALLKSGD
                     HIVSSHSIFGTTTTLFDKYLP"
     gene            complement(561..1703)
                     /locus_tag="VIW27_13675"
     CDS             complement(561..1703)
                     /locus_tag="VIW27_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740772.1"
                     /translation="MNLLPRVCLLPVILVLQFGCASYAKYPVTGTLLDETIDTTVDSA
                     EAQYYLNHYLQGERLDAELDRRIDLVYRDHPSPIPDRDDLRRVADGFSNDFAALFLAD
                     RLWQDQQNRKVQRIFNRYLGLSKRELFAPPSATEDLLVLLVPGWNYVDNGHVTGSDFA
                     APRRLIDRLGIDNELVMVPSNGSVQQSAEVIAERIVEQGADGRRIILVGASAAGPAIH
                     YTLGKLLEPEQLAPVIAWVNLGGILQGSPLIDRFQQYPQKLLLDLVVAFRGWDIDEIM
                     SMSAAQSRQRVKTLQLPQNLLVINYLGLSLTGSLSSLSAYKYPLIAGEGPNDGLTPLS
                     DIIAPGSMTLVATRSDHYFGEDPLIDDKTIAMLKTILELIGDSSGP"
     gene            complement(1928..2467)
                     /locus_tag="VIW27_13680"
     CDS             complement(1928..2467)
                     /locus_tag="VIW27_13680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5740773.1"
                     /translation="MRWQDISRDLAAVLQGDALLAAQRPIIVVDSAQQCLHRLDIDAA
                     NNRSYPVSTAANGIGNRVDSYMTPHGIHRIEQKIGGDEPRGMVFEARKPTGRVASNLD
                     NRDQDEITSRILWLGGLEPGINRDGDCDTRSRYIYIHGTSDERRIGQPVSAGCIRMNN
                     ADVIELFEEVEVGDLVIIR"
     gene            complement(2467..>3835)
                     /gene="purF"
                     /locus_tag="VIW27_13685"
     CDS             complement(2467..>3835)
                     /gene="purF"
                     /locus_tag="VIW27_13685"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY5740774.1"
                     /translation="QVSDVFHTRHMLKLTGTMGIGHVRYPTAGCSSSAEAQPFYVNSP
                     YGISLAHNGNLTNMEQLKAELYQSDLRHINTESDSEVLLNVFAHELQRCGSLKLTPQE
                     IFTAVTETHKRIEGAYAVVLMLTGKGILGFRDPHGIRPIVYGKRETGGGTEYMIASES
                     VALQASGFELIGDLQPGEAVYIDDSGRVHRRQCAAKTKLRPCIFEYVYLARPDSIIDD
                     IYVYKARLRMGVKLARKILRQMPEHDIDVVIPIPDTSRPAAMELAFHLGVKYREGFIK
                     NRYIGRTFIMPGQKMRKRSVRRKLNPIDIEFKGKTVLLVDDSIVRGTTSQQIIQMARE
                     AGAKKVYMASAAPPVRYPNVYGIDMPAADELVAHDRTEEEIGAYIGADWLIYQDLEDL
                     VDAVQKGNRSIKKFDCSCFDGKYVTNTVDDDYLNRISSLRNDSAKEKSDEQVTVIGME
                     LHNNA"
BASE COUNT          726 a         1137 c         1188 g          784 t
ORIGIN      
        1 cggcaagtac ttgtcgaaca gcgtagtcgt ggtgccgaag atcgagtgcg aactgacgat
       61 atgatcgcca gacttgagca gcgccaggca aacgctggtg atcgccgcca tgcccgacgc
      121 ggtcgcgaca caggcctcgc cgccctcgag cgcggcgaga cgctgttcaa aggcccgcgt
      181 ggtgggattg gtaaagcgcg aatagatatt gccgggctgc tcgccggcga atcgcgcggc
      241 cgcctgcgcc gcgttttcga acacgaagct cgaggtcgcg aatatcgctt cgctatgctc
      301 gagttcggcc gtacgcagct gaccgcttct gacggcgagc gtatcaaaac cgaggttgtc
      361 aaggttcacg gagtttctcc aataatcgaa aaaactcggc agaaaatagt acggaaagac
      421 ttcgggacta ccctctttag cacatttgtt ttggaagccg tgaaaacccg cttcgaggcg
      481 cctgcaagcc gagaaacaaa tcggcgccga acgcttaaga tatgcgctcg gctttttcct
      541 gtcaagcgct cagcggccgc tcacgggccg ctcgaatcac cgatcagctc gaggatggtt
      601 ttcagcatcg cgatggtctt gtcgtcgatc agcgggtcct cgccgaagta atggtcgctg
      661 cgggttgcga ccagcgtcat gctgccgggg gcgatgatat cggacagcgg cgtcaggccg
      721 tcgttggggc cctcgccggc gatgagcgga tacttgtagg ccgacaggct gctgaggctg
      781 ccggtcagcg acaggcccag gtagttgatc accagcaggt tctgcggcaa ttgcagcgtc
      841 ttcacccgtt gcctcgactg cgcggccgac atgctcatga tttcatcgat atcccagccg
      901 cgaaaggcaa ccaccagatc gagcagcagc ttttgcggat attgctggaa gcggtcgatc
      961 agcgggctgc cctgcaggat tccgcccaga ttgacccagg cgatgaccgg ggccagttgt
     1021 tcgggctcta gcagcttgcc cagcgtatag tgaatcgccg gtccggcggc gctggcgccg
     1081 accagaatga tcctgcgacc gtcggcgccc tgttccacga tccgctcggc gatgacctcg
     1141 gcggattgct gcaccgagcc gttcgacggc accatcacca gttcgttgtc gatgccgagc
     1201 cgatcgatca gccgccgcgg agcggcaaaa tcggagccgg tgacatgccc gttatcgacg
     1261 tagttccagc ccggtaccag cagcaccagc agatcctcgg tggccgatgg cggcgcaaaa
     1321 agctcgcgct tcgacaaccc aaggtagcgg ttgaaaatcc tctgtacctt gcgattttgc
     1381 tggtcttgcc acagccggtc ggcgaggaac agcgccgcga agtcattcga gaagccgtcc
     1441 gcaacccggc gcaagtcgtc gcggtcagga atcggcgacg ggtggtcgcg gtagacgaga
     1501 tcgatgcgcc tgtcgagttc ggcatcgagc cgttcgccct gcaggtaatg gttgagatag
     1561 tactgggcct cggcggaatc gaccgtggtg tcaatggttt cgtcgagcaa ggtccccgtt
     1621 accgggtatt ttgcgtaaga tgcgcaaccg aactgcaata ccaggatcac cggcagcaga
     1681 cagacgcgcg gcaacagatt catagctaag ccttgttgtt attttggcct ggcaagcctt
     1741 tcgttcgccg gcaacgacgg gtttcgatcg acccggcatc cccctcatcc cgatcttcgg
     1801 gtgatccgat tctacattct tgcggcgcga aatgacaaat tcgccgggcg cttgcgcaaa
     1861 gacgaggaac cgctctgcgc ggcaagcggc cggcaaggcc ggcactggtt tgcgggcgcg
     1921 ggtatggcta cctgatgatc accaggtcgc cgacctcaac ctcttcgaac agttcgatga
     1981 cgtcggcgtt attcatgcgg atgcagccgg cggaaaccgg ctggccgatg cgccgctcgt
     2041 cgctggtgcc gtggatatag atatagcggg agcgggtatc gcagtcgcca tcgcgattga
     2101 taccgggctc gaggccgccc agccaaagga ttcgcgaagt gatctcatcc tggtcgcggt
     2161 tatcgagatt gctcgctacc cggccggtgg gcttgcgggc ctcgaatacc atgccgcgcg
     2221 gctcgtcgcc gccgatcttc tgttcgatgc ggtgaatgcc atgcggggtc atatacgaat
     2281 ccacgcggtt gccgattccg ttggcggccg tggacaccgg gtaactgcga ttgttggcgg
     2341 catcgatatc gagccggtga aggcactgct gcgccgaatc gacaacaatt atcggccgct
     2401 gtgcagccag cagcgcgtca ccctgcagga cggcagcgag gtcacgactg atatcctgcc
     2461 agcgcatcag gcgttgttgt gcagttccat gccgatgacg gtcacctgct cgtcactctt
     2521 ttcctttgcc gaatcgtttc tcaggctgct gatcctgttc aggtaatcat cgtcgacagt
     2581 attggtgaca tacttgccgt cgaaacacga acaatcgaat ttcttgatgc ttcggttacc
     2641 tttttgcacc gcgtcgacga gatcctcgag atcctggtag atcagccagt cggcaccgat
     2701 ataggcgccg atttcttcct cggtacggtc gtgggcaacc agttcgtcgg ccgccggcat
     2761 gtcgataccg tagacgttcg gatagcgcac cggcggcgcc gccgaggcca tgtaaacctt
     2821 ttttgcccct gcctcgcgcg ccatctggat gatttgctgc gaggtggtgc cgcggacgat
     2881 cgaatcatcg accagcagta cggtcttgcc cttgaactcg atatcgatcg ggttgagctt
     2941 gcgacgcacc gagcgcttgc gcatcttttg cccgggcatg atgaaggtgc gcccgatata
     3001 gcggttcttg atgaacccct cgcggtactt gacgccgagg tggaaggcga gctccatcgc
     3061 cgccggccga ctggtgtccg ggatcggaat cacgacgtcg atatcgtgct ccggcatctg
     3121 tcgcaagatc ttgcgcgcca gcttgacgcc catccgcagg cgcgccttgt agacatagat
     3181 atcgtcgatg atcgaatccg ggcgcgcgag gtaaacatac tcgaagatgc acggcctgag
     3241 cttggtcttg gcggcacact ggcggcgatg cacgcgaccg gaatcgtcga tatagaccgc
     3301 ttccccgggc tgcaggtcgc cgatcagttc gaaaccggaa gcctgcagcg ccacactctc
     3361 ggaagcgatc atgtattcgg tgccgccgcc ggtttcgcgc ttgccgtaaa cgatcggccg
     3421 aataccgtgc gggtcgcgaa acccgaggat tcccttgccg gtcagcatca ggaccaccgc
     3481 ataggcgccc tcgatgcgct tgtgcgtctc ggtcaccgcg gtaaatattt cctgcggcgt
     3541 cagcttcagg ctgccgcagc gctgcaattc atgggcgaag acgttgagca atacctccga
     3601 gtccgactcg gtgttgatat ggcgcaggtc ggactggtag agctccgcct tcaattgctc
     3661 catgttggtc aggttgccgt tatgggccag gctgatgccg tacggtgaat tgacgtagaa
     3721 cggctgcgcc tcggcggacg acgaacagcc ggcggtcggg taacgaacgt ggccgatgcc
     3781 catggtgccg gtcagcttga gcatgtgccg ggtatggaac acgtcgctga cctgg
//
LOCUS       DATCZV010000608         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_101621, whole genome shotgun sequence.
ACCESSION   DATCZV010000608 DATCZV010000000
VERSION     DATCZV010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101621"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VIW27_13690"
     CDS             complement(<1..152)
                     /locus_tag="VIW27_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HEY5740775.1"
                     /translation="MYLYADLLQRAVEERPVRVGLIGAGKFGSMFLAQAPTTPALEVA
                     AIADLDP"
     gene            448..1437
                     /locus_tag="VIW27_13695"
     CDS             448..1437
                     /locus_tag="VIW27_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740776.1"
                     /translation="MNNHTRRLFLKLTAAGIAASTLPARVFAAGFPERNLSVYIPTRA
                     GGGADRNFRNFGGVWKNYLGVDLEPGFYPGAAGRVGYETYMGKAADDCHDLIFGNMGP
                     ESLNWVVKEPTFSLDDYFYFLMVDADPGCVFISDKSPMKSLDDVIAEGKKRTLTVATS
                     RLAHPASLGMLVLANKTGMKVNLVPLSGGKNTRNGVLTGEVDLGVLPATTAMGRKGIT
                     VLGLFDEKNLVPESTPGAVLINTEYRLGIPPIAAGARAFGIKTAAAEKHPDRFKLLQE
                     TGAKVFGDPAYKQAVTKGKGSWELISPGGIAECRQYVDNITALGREYRSLLTG"
     gene            1569..>2071
                     /locus_tag="VIW27_13700"
     CDS             1569..>2071
                     /locus_tag="VIW27_13700"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY5740777.1"
                     /translation="MAMQQEPPREPRNILPDFIIPALALVFTIYYLTTITEVPWISQA
                     SAIVVGSLLTLSILAFVLRSVLRIRRGEETLELRDTLHTLLGRVPVSSRRIGLLALAV
                     GYVWIIDYLGFTLTTFGFVFAAIILLSSLANWKRALAVSVSSAVVGYVVFLYFFNTRF
                     PSGPIED"
BASE COUNT          370 a          636 c          647 g          418 t
ORIGIN      
        1 gggtcgaggt cggcgatcgc cgcgacctcg agcgctggcg tggtcggcgc ctgggcgagg
       61 aacatgctgc cgaacttgcc ggcgccgatc aggccgaccc tgaccggccg ctcttcgacg
      121 gcgcgctgta acagatccgc atagagatac atcgcgttca ggccgcctgc tgtttgccgg
      181 cggcgattgc gcgcggggta ccttcgattg gtgcctgact ggcttgcatc ggcatgcttc
      241 tggtgttgac ggggcggtca ttctaccccc gggcgacggt ttttcacagg acactttggc
      301 cgggtcgtat cgatattgag caactgcgcc cgcctgcggt tgcgtggcca tcctggtgaa
      361 attgattgcg acccctgttt cggttatctt gcttgctggt cgctgaaaat agaatgcgaa
      421 aagcgataca caactggagg attatcaatg aataaccaca cgcgtcgttt atttctgaaa
      481 ctgaccgccg cgggtatcgc ggcgtcgacg ctgccggcgc gagtcttcgc cgccggtttc
      541 ccggagcgca acctcagcgt ttacattccg acccgtgcgg gcggcggcgc cgatcgcaat
      601 ttccgcaact ttggcggtgt ctggaaaaat tatctgggcg tcgatctcga gcccgggttt
      661 tatcccggcg ccgcgggccg cgtcggctac gaaacctaca tgggcaaggc cgccgacgat
      721 tgccacgacc tgattttcgg caacatgggt ccggagtccc tgaactgggt cgtcaaggag
      781 ccgacatttt cgctcgacga ctatttctac ttcctgatgg tcgacgccga cccgggctgt
      841 gttttcatca gcgacaagag cccgatgaaa tccctcgacg acgtcatcgc cgagggcaaa
      901 aagcggacgc tgacggtggc gaccagccgc ctcgcacacc cggcatcgct gggtatgctg
      961 gtgctggcca acaagaccgg catgaaggtc aacctggtac cgctgtcggg cggcaagaat
     1021 acgcgtaacg gcgtactcac cggtgaggtc gacctcggcg tgctgccggc caccacggcc
     1081 atgggccgca aggggattac cgtgctcgga ctgttcgatg aaaagaatct ggtgcccgag
     1141 agcaccccgg gcgcggtgtt gatcaacacc gagtacaggc tcggcattcc gccgatcgcc
     1201 gccggcgcgc gcgcctttgg catcaagacc gcggcggccg aaaaacaccc cgaccgtttc
     1261 aagctgttgc aggaaaccgg cgccaaggtg ttcggcgatc cggcctacaa gcaggccgtc
     1321 accaagggca agggctcctg ggaactgatc agcccgggcg ggattgccga atgccggcaa
     1381 tacgtcgaca acatcaccgc actgggccgc gaataccgca gcctgctgac cgggtaagtc
     1441 gagacgcaaa tcaccggcag ccgggcacac acccggctgc tgttaccgat gccggctgcc
     1501 cggcgcatgc cttcgcgagc cgccccgcgg cacgaggatt gtttcgatta cccgtgtttg
     1561 aaagataaat ggcgatgcaa caggaaccac cccgggaacc gcgcaatatt ctgcccgatt
     1621 tcatcattcc ggcgctggcg ctggtgttca cgatttatta cctgaccacg attaccgagg
     1681 taccgtggat ctcgcaggcc agcgcgatcg tggtcggcag cctgctgacg ctgtcgatcc
     1741 tggcgttcgt gttgcgcagc gtgttgcgca tccgtcgcgg tgaggaaacc ctcgaattgc
     1801 gcgataccct gcacacgctg ctcggtcgcg tgccggtcag cagccggcgc atcggcctgt
     1861 tggcgctggc ggtaggctat gtctggatca tcgactacct cggcttcacg ttgacgactt
     1921 tcggcttcgt gtttgcggcg atcatactgc tgtcgtcgct ggccaactgg aaacgtgcgc
     1981 tcgcggtatc ggtatcgagc gcggtggtcg gctacgtcgt gttcctgtat tttttcaata
     2041 cccggtttcc gagcggtccg atcgaggact g
//
LOCUS       DATCZV010000609         7431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_313670, whole genome shotgun sequence.
ACCESSION   DATCZV010000609 DATCZV010000000
VERSION     DATCZV010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313670"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..576)
                     /gene="rpsG"
                     /locus_tag="VIW27_13705"
     CDS             complement(106..576)
                     /gene="rpsG"
                     /locus_tag="VIW27_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5740778.1"
                     /translation="MPRRREVPKREILPDPKFGDLQIAKFINMIMRNGKKSTAERIMY
                     YALDNVSEKSKLDSVELMEKALENVRPMVEVKSRRVGGATYQVPVEVRASRRNTLAMR
                     WIIDAAQKRGEKTMAGKLAGELLDASENKGTAVKKREDTHRMAEANKAFAHFRW"
     gene            complement(598..972)
                     /gene="rpsL"
                     /locus_tag="VIW27_13710"
     CDS             complement(598..972)
                     /gene="rpsL"
                     /locus_tag="VIW27_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5740779.1"
                     /translation="MATVNQLVRKPRQSRRSKSNVPALEASPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTTGYEVASYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHVVRGSL
                     DTQGVPSRRQGRSKYGAKRPKS"
     gene            complement(1286..5503)
                     /gene="rpoC"
                     /locus_tag="VIW27_13715"
     CDS             complement(1286..5503)
                     /gene="rpoC"
                     /locus_tag="VIW27_13715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041595058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY5740780.1"
                     /translation="MKDLLNLLKKQGADEDFDHIRVGLASPDMVRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKVFGPVKDFECLCGKYKRLKHRGVVCEKCGVEVTQTKVRRERM
                     GHIELACPIAHIWFLKSLPSRIGLLLDMTLRDIERVLYFEGFVVIDPGMTTLEKGQLL
                     TDEAYLEAIEEFGDDFNAKMGAEAVFELLQGVDLNHEAQALREEIESTKSETKFKRLS
                     KRLKLVEAFIDSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELNAPDIIVRNEKRMLQESVDALLDNGRRGRAITGTNKRPLKSLSDMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLQLRGLAT
                     TIKAAKKLVEREVAEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQLH
                     PLVCTAFNADFDGDQMAVHVPLSIEAQLEVRALMMSSNNILSPANGKPIIVPTQDIIL
                     GLYYMTRDKINVQGEGMVFADAKEVLRAYHGKVIELQARIKCRVSEYHRDEDGNRIEN
                     RSLVDTTVGRAILSEMLPDGLPFSLINQTLDKKAVSKLIDACYREIGLKDTVILADQL
                     MYTGYHYATRSGISIGVNDMIVPDEKADLIKAAEAEIKDIEKQWQTGLVTSGERYNKV
                     VDIWSHANDKVARAMMEKIGKEIVVDAEGNEVEQKSLNSIFVMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIENAITANFREGLDVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVSQDLVVNMDDCGTEDGIALRPVIEGGDIVVDLGERVLGRVIQQDLKDDKGKVV
                     IKRGALLEEREVELIHELGVDELLVRSPVTCEARHGICVQCYGRELARGHKVGKGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGAASSSASVNSIEVKNTGTVRFHNVKVVKNSTKRL
                     IAVSRSGEIGVVDEATGREKERYKIPYGSEFMVEDGQQVNVGDTAATWDPHMHPVITE
                     VAGKVKFTDFVEAVTVQHRVDEMTGLTTIEVMDDSIRSNTGKELRPTVELIDGKGKVL
                     CYAGTNIPVHYMLPGGAFVNVEDGAKIKVGDVLAKIPQESSKNRDITGGLPRVADLFE
                     ARKPKEPAILSEMTGTVSFGKETKGKKRLVITDAEGESIETLIPKFRHINVFEGEHVE
                     KGEILVDGELDSHDIVRVKGVRDLTNYMVNEVQDVYRLQGVAINDKHIEVIVRQMLRK
                     VTIVDGGDTRLLRGDQVDKARVLDMNDKAIAMNKKPATFEQDLLGITKASLVTESFIS
                     AASFQETTRVLTEAAVRGAKDDLRGLKENVIVGRLIPAGTGQTYHDDRRRKRLQPAMP
                     VMPGSDVFESSAEGAAEAGSEEATVAE"
     gene            complement(5507..>7431)
                     /gene="rpoB"
                     /locus_tag="VIW27_13720"
     CDS             complement(5507..>7431)
                     /gene="rpoB"
                     /locus_tag="VIW27_13720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5740781.1"
                     /translation="GTTVKALRGGVVDSVDASRVVVRVHDSETREGEPGVDIYNFTKY
                     TRSNQNTCINQKPLVRPGDKIEAGDVLADGASTDMGELALGQNMQVAFMPWNGYNFED
                     SILLSERVVKEDRFTTIHIEELQCLARDTKLGVEEITADIPNVSEGLLSKLDESGVVY
                     VGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMKSGVTGTVVD
                     VRVFTRDGVEKDARALDNERIEIDALKKDLDDQFRIIEENIYQRVARLATGKKASGGP
                     GGIKKNGVITADYLASIGRDQWSKIRVVDDKLAKQMEALSEQLEAQRAEFDRLFEEKK
                     KKVTAGDDLKPGVLKMVKVYVAVKRRMQPGDKMAGRHGNKGVVSMIVPEEDMPYREDG
                     TPVDVVLNPLGVPSRMNVGQILEAHLGWAAAGLGHKIGEMLDRQRKVEELKTFLRKIY
                     NNNSEKLKMKELSDDDVVNLCENLRAGVPMATPVFDGAEEEEIKNMLALAELPETGQT
                     TLIDGRTGEPFDRPVTVGYMYMMKLNHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQ
                     RFGEMEVWALESYGAAYTLQEMLTVKSDDVNGRNKMYKNIVDGDFSMEAGMPESFNVL
                     LKEIRSLSLNIELEQE"
BASE COUNT         1244 a         2355 c         2209 g         1623 t
ORIGIN      
        1 ggtggtcttg ccggcatcga tgtgcgcgat gatgccgata tttcggtact gcgtaatcgg
       61 tgtcgtgcgc gccacctcag aatcccttgg tcagtgaatt acgccttacc agcggaagtg
      121 agcgaacgcc ttgttagcct cggccatgcg atgcgtgtct tcacgcttct tgactgcggt
      181 tcccttgttt tccgatgcat ccagcagctc gccggccagc ttgccggcca tggttttctc
      241 accgcgcttc tgcgccgcgt cgataatcca gcgcatcgcc agcgtgttac gccgcgaagc
      301 acgcacctcg accggcacct ggtaggtcgc gccgccgacg cgacgggatt tgacctcgac
      361 catcgggcgc acgttttcga gtgctttttc catcagctcg accgagtcca gcttgctctt
      421 ctcggacaca ttgtcgagtg cgtaatacat gatccgctcg gcagtggatt tcttgccgtt
      481 gcgcatgatc atgttgatga acttggcgat ctgcaggtca ccgaacttcg gatccggcag
      541 aatttcgcgt ttcgggactt ctctacgtct gggcattaaa gcttaacctt gaatgaatta
      601 tgacttggga cgcttggcgc cgtatttgga acggccctgg cgccggctgg gcacgccctg
      661 ggtatcgagg ctgccacgca ccacgtggta gcgcacgccc ggcaggtctt ttacacgacc
      721 gccgcgcacc agcaccaccg agtgctcctg cagattgtgg ccctcgccac cgatgtaaga
      781 ggcaacctca taacccgtcg tcaggcgcac gcgagcgacc ttgcgcagcg cggagttggg
      841 ttttttcggc gtcgtggtat agacgcgggt gcaaaccccg cgcttctgcg gcgacgcttc
      901 gagtgccggc acgttgctct tgctgcgccg tgactggcgc ggcttacgta ccagctggtt
      961 gacagttgcc atctattcga ctctcccggg agtacagttt ttcaaggatt accggaatct
     1021 gcgctaggca gggattcccg cggcactcga tcgtgccgcc gtctttcgat cacccctgtg
     1081 atcgaaaaac gcggcgcaac gcaagatcac gttgacgcat tttcgaagcc atgccaggtc
     1141 ggacctggcg ggccgtgcct tcccgggatc gaccgggact gactggcaca gggcttccac
     1201 cagcaacagc aggctataca atacgtatag cctgccatcg tgggaggcgg aattttaggg
     1261 gcgaaaccgc gccctgtcaa cccgcttatt cggcgaccgt ggcttcttcg gaacccgcct
     1321 cggcggcacc ctcggcactg ctttcgaaca catcgctacc cggcattacc ggcatcgccg
     1381 gctgcaagcg cttgcggcga cgatcatcgt ggtaagtctg cccggtaccc gccgggatca
     1441 agcgaccgac gatgacgttt tccttcagtc cgcgcaaatc gtccttggca cccctgaccg
     1501 cggcctcggt gagcacgcgc gtggtttcct ggaaagaggc cgcggaaatg aacgactcgg
     1561 taaccagcga agccttggtg atgccgagca gatcctgttc gaaggtcgcc ggcttcttgt
     1621 tcatcgcgat cgccttgtcg ttcatgtcga gcacgcgcgc cttgtcgacc tggtcaccac
     1681 gcaacaggcg ggtatcgcca ccgtcgacga tcgtgacctt gcgcagcatc tgccgcacga
     1741 tgacctcgat atgcttgtcg ttgatcgcca caccctgcag ccggtaaacg tcctgcacct
     1801 cgttgaccat gtaattggtc agatcgcgga cgcccttgac gcgcacgata tcgtgcgaat
     1861 cgagttcgcc gtcgaccagg atctcgcctt tttcgacgtg ctcaccctcg aacacgttga
     1921 tatggcggaa cttcggaatc agggtctcga tcgactcgcc ttctgcatcg gtgatcacca
     1981 ggcgcttctt gcccttggtt tccttgccga aggagacggt acccgtcatc tccgagagga
     2041 tcgcgggctc tttcggcttg cgcgcttcga acaggtcggc aacccgcggc agaccaccgg
     2101 tgatatcgcg gttcttagac gattcctgcg gaatcttggc gagcacgtcg ccgaccttga
     2161 tcttggcgcc gtcctcgacg ttgacgaaag ccccgccggg tagcatgtag tgcaccggaa
     2221 tgttggtgcc cgcgtagcaa agcaccttgc ccttgccgtc gatcagttcc accgtcggcc
     2281 gcaattcctt gccggtattg gagcggatcg aatcgtccat gacctcgatc gtggtcaaac
     2341 cggtcatttc gtcgacgcga tgctgtacgg tgaccgcctc gacgaaatcg gtgaacttga
     2401 ccttgcccgc tacctcggtg atcaccgggt gcatgtgcgg atcccaggtc gctgccgtgt
     2461 caccgacgtt gacctgctgg ccgtcctcga ccatgaactc ggagccatac gggatcttgt
     2521 aacgctcctt ctcgcggccg gtggcttcat cgacgacccc gatctcgccg gagcgcgata
     2581 ccgcgatcag gcgcttggtc gaattcttga ccaccttgac gttgtggaag cgcacggtac
     2641 cggtgttctt gacttcgatg ctgttgaccg atgccgagct gctcgcggca ccgccgatat
     2701 ggaaggtccg catggtcagc tgggttcccg gctcgccgat cgactgcgcg gcgataacgc
     2761 ccacggcctc acccttgccg accttgtggc cgcgcgccag ctcgcgcccg taacactgca
     2821 cgcagattcc gtggcgcgcc tcgcaggtga ccggggagcg gaccagcaac tcgtccacgc
     2881 cgagctcgtg gatcagctcg acctcgcgct cctcgagtaa cgcgccgcgc ttgatcacga
     2941 ccttgccctt gtcgtccttg agatcctgct ggatgacgcg gccgagcacg cgctcgccga
     3001 gatcgacgac gatgtcgccg ccctcgatga ccgggcgcag cgcgatgccg tcctcggtac
     3061 cgcaatcgtc catgttgacc accaggtcct gtgaaacatc gaccaggcgg cgggtcaggt
     3121 aacccgagtt ggcggttttc agcgcggtat cggcgagacc cttgcgtgca ccgtgcgtgg
     3181 agatgaagta ctgcagtacg tcgagaccct cgcggaagtt ggcggtgatc gcattctcga
     3241 tgatcgagcc gtcgggcttg gccatcaggc cgcgcatgcc ggccagctgg cgaatctgcg
     3301 ccgcggaacc ccgcgcgccg gagtcggcca tgacgaagat cgagttcagc gatttctgct
     3361 cgacctcgtt accctcggca tcgacgacga tttccttgcc gatcttttcc atcatcgcgc
     3421 gcgccacctt gtcgttggca tgcgaccaga tatcgacgac cttgttgtaa cgctctcccg
     3481 aggtcaccag gccggtctgc cactgtttct cgatgtcctt gatttcggct tccgcggcct
     3541 tgatcaggtc ggccttttcg tcgggaacga tcatgtcgtt gacgccgatc gagatacccg
     3601 acctggtggc atagtgataa ccggtgtaca tcagctggtc ggcgaggata accgtgtcct
     3661 tgaggccgat ctcgcgatag caggcatcga tcagcttgga aaccgctttc ttgtccagcg
     3721 tctggttgat cagcgagaac ggcaggccgt ccggcagcat ttcgctgagg atcgcgcggc
     3781 cgacggtggt atccaccagg gaacgattct cgatgcggtt gccgtcctcg tcgcggtgat
     3841 attcggagac ccggcacttg atgcgcgcct gcagctcgat caccttgccg tggtaggcgc
     3901 gcagtacttc cttggcgtcg gcgaatacca tgccctcgcc ctgcacgttg atcttgtcgc
     3961 gggtcatgta gtacaggccg aggatgatgt cctgggtcgg cacgatgatc ggcttgccgt
     4021 tggctggcga cagaatgttg ttcgacgaca tcatcagcgc gcgcacttcg agctgcgcct
     4081 cgatcgacaa cggcacgtgg accgccatct ggtcgccgtc gaagtcggcg ttgaacgcgg
     4141 tacagaccag cgggtgcagc tggatcgcct taccctcgat caggatcggc tcgaaagcct
     4201 ggatgccaag gcggtgcagc gtcggcgccc ggttgagcat gatcgggtgc tcgcggatga
     4261 cttcctcgag aatatcccag acctcggcga cctcgcgctc gaccagcttc ttggctgcct
     4321 tgatggtggt cgccaggccg cgcaactgca gcttggagaa aatgaacggc ttgaacagtt
     4381 ccagcgccat cttcttcggc aggccgcact ggtgcagctt cagcgtcggg ccgaccacga
     4441 tgaccgagcg gccggagtag tcgacgcgct tgccgagcag gttctggcga aagcgcccct
     4501 gcttgccctt gatcatgtcg gacagcgatt tcagcgggcg cttgttggtg ccggtgatcg
     4561 cgcggccgcg acggccgtta tccagcagcg cgtcgaccga ctcctgcagc atgcgctttt
     4621 cgttgcgcac gatgatgtcc ggcgcattga gttcgagcag gcgcttgagg cggttgttgc
     4681 ggttgatcac gcggcggtac aggtcgttca ggtccgaggt cgcgaaacgg ccgccgtcga
     4741 gcggcaccag cggacgcagg tcgggcggca gcaccggcag cacggtcatc accatccact
     4801 cgggcttgtt gcccgagtcg ataaaagcct cgaccagctt gagccgcttg gacaggcgct
     4861 tgaacttggt ttccgacttg gtgctctcga tttcctcgcg cagcgcctgc gcctcgtggt
     4921 tcagatccac gccctgcagc agttcgaaca ccgcctcggc acccatcttg gcattgaagt
     4981 cgtcgccgaa ctcctcgatc gcctcgaggt aagcctcgtc ggtcagcagc tggccttttt
     5041 ccagcgtcgt catacccgga tcgatgacca cgaagccctc gaagtagagc acgcgctcga
     5101 tgtcgcgcaa ggtcatgtcg agcaacagac cgatgcgtga cggcagcgac ttcagaaacc
     5161 agatgtgcgc aatagggcac gccagttcga tgtgtcccat gcgctcgcgg cgcactttgg
     5221 tctgggtgac ctcgacgccg cacttctcgc agaccacgcc gcggtgcttg agacgcttgt
     5281 acttgccgca caggcattcg aaatccttga ccgggccgaa taccttggcg cagaacaggc
     5341 cgtcgcgctc gggcttgaag gtacggtagt tgatggtctc cggcttcttg acttcgccgt
     5401 aggaccagga acgcaccatg tctggcgaag ccagtccgac cctgatgtgg tcgaaatcct
     5461 cgtccgcacc ctgtttcttc aatagattca gcaagtcttt catggcttat tcctgttcga
     5521 gttcgatgtt caggcttaag gatcggatct ccttgagcag aacgttaaac gattcgggca
     5581 ttccggcttc catgctgaaa tcgccgtcga cgatattctt gtacatcttg ttacggccgt
     5641 tgacgtcgtc cgacttgacg gtcagcattt cctgcagcgt gtatgccgcg ccgtaggatt
     5701 ccagtgccca gacttccatc tcgccgaaac gctggccgcc gaactgcgcc ttgccgccca
     5761 gcggctgctg ggtgaccagg ctataaggcc cggtggagcg cgcgtgcatc ttgtcgtcga
     5821 ccaggtggtt gagtttcatc atgtacatgt agccgaccgt gaccgggcga tcgaagggct
     5881 caccggtgcg gccgtcgatc agcgtggtct ggccggtctc gggcagctcc gcgagcgcca
     5941 gcatgttctt gatttcttct tcctcggcac cgtcgaacac cggcgtcgcc atcggcacgc
     6001 cggcacgcag gttttcacac aggttaacca cgtcgtcgtc gctcaattcc ttcattttca
     6061 gcttttcgga attgttgttg tagatcttcc tgaggaaggt cttcaattcc tcgaccttgc
     6121 gctgccggtc gagcatttcg ccgatcttgt ggccgagtcc ggccgcggcc cagccgaggt
     6181 gtgcctcgag aatctggccg acgttcatgc gcgacggcac gccgagcggg ttcaatacca
     6241 cgtccaccgg ggtaccgtcc tcgcggtaag gcatgtcttc ctcgggcacg atcatcgaca
     6301 ccacgccctt gttgccgtga cggccggcca tcttgtcgcc aggctgcatg cggcgcttga
     6361 ccgcaacgta aaccttgacc atcttcagca cgcccggctt gagatcgtcg ccggcggtga
     6421 ccttcttctt cttttcctcg aacaggcgat cgaactcggc gcgctgcgcc tcgagctgct
     6481 cactcagtgc ttccatctgc ttggccagct tgtcgtcgac gacgcgaatc ttcgaccact
     6541 ggtcgcggcc gatggatgcg aggtaatcgg cggtaatcac gccgttcttc ttgatgccgc
     6601 cggggccgcc gctggctttc ttgccggtgg ccagccgcgc aacgcgctgg tagatgtttt
     6661 cctcgatgat acggaactgg tcgtcgaggt cctttttcag cgcgtcgatc tcgatgcgct
     6721 cgttatccag cgcgcgggcg tccttttcga cgccgtcgcg ggtgaaaacg cgtacgtcga
     6781 cgacggtgcc ggtgacgccc gacttcatgc gcagcgaggt atccttaacg tccgacgcct
     6841 tttcgccgaa gatcgcgcgc agcagcttct cttccggggt cagctgggtc tcgcccttcg
     6901 gcgtgacctt gccgaccagg atatcgccgg gcttgacctc ggcaccgaca tagaccacgc
     6961 ccgactcgtc gagcttggac agcaggccct cgctgacatt cggaatgtcg gcggtgattt
     7021 cctcgacgcc gagcttggtg tcgcgcgcca ggcactgcag ttcctcgata tggatcgtcg
     7081 tgaagcggtc ttcttttacc acacgctccg acaacaggat cgaatcctcg aagttgtagc
     7141 cgttccacgg catgaacgcg acctgcatgt tctgccccaa cgcgagctcg cccatgtcgg
     7201 tggaagcgcc gtcggcgagc acgtcgccgg cctcgatttt atcgcctgga cgcaccagcg
     7261 gtttctggtt gatgcaggtg ttctggttgg aacgcgtata cttggtgaaa ttgtagatat
     7321 cgacgcccgg ttcgccctcc cgggtttccg agtcgtgcac gcgcaccacc acgcggctgg
     7381 cgtcgaccga gtcgaccacg ccgccgcgca gggccttgac cgtggtaccc g
//
LOCUS       DATCZV010000610         5426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_684959, whole genome shotgun sequence.
ACCESSION   DATCZV010000610 DATCZV010000000
VERSION     DATCZV010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684959"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2894)
                     /locus_tag="VIW27_13725"
     CDS             complement(<1..2894)
                     /locus_tag="VIW27_13725"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY5740782.1"
                     /translation="MNDYMRKYLARILATAALTAMPLYQAHGAPGNLPPAPLFLSTLV
                     ESNVYFTLDDSGSMDWGPILRSDTGLGTATGLPIINGRYRAYYTPSFSHLYTNADGVY
                     VLPPSNGANSVWDRSWVVRNHLANPNYYNPSVTYKPWAGTNPDGSPIYADANPSKALK
                     HPGQPLGEFVNLTINHSFTEDAATVTNYWIPTYHVWIDSDNDGVLEVTDANTRIEIAP
                     NTTEMQNFANWFQYYRTRMHATKAIVGDTINSTDASRMGASWFNAGHIADVATMKDAS
                     NKRSLQTALYNLQNQIFGSPTRSALKAAGDYFMNATGTGPILPASEGGECQQNFNIVM
                     SDGFWNGNANPAVGNTDADGGTSDTAFDGNAAQSNDGGNYADGASNTLADVAMRNYET
                     DLRTDLANRVPQQATIDEADHQHLVTYSVGFGINGTLDPATTDPLAAGFSWPAPSKNS
                     NTTVDDLWHASYNGRGTYLSAQNPIELQEALSAAITDISQRTGTTAAVALNTARLSTE
                     AVVYLAQFNSNRWQGNLLAYPIVDLDTGALANTPKWDAASRLNGRDLVSKPRTIISYS
                     DAPAVRDGVPFQWADISIGMRDDLKTNALGGVDSDTIGRARLDYLRGDQSNEGTGFFF
                     RDRLSLLGDIVNSGPVFVGEPTINWPDVAPFPEGLNAYSKFKNGTAKDRKKIVYVGAN
                     DGMLHAFDDASGDEVLAYVPGIFNSNAIGQGLHYLTESNYVHRFYVDQTPAVSDAFIS
                     TGGITQWRTVLVDGLRSGGRGLFALDVTEPSLFNSEANADKIVMWEFTSDDDSDLGYT
                     FSRPFIGLANNGKWVAIFGNGYNDLGSGEAHLYIVDLEGGVDGNWTVGDYWKIPTGVG
                     DPTNRNGLATPALADIDGNGTVDRVYAGDLQGNMWVFDISASNPSQWGFAYGSNATPR
                     PLFKAPVGQPITAKPVLARHPTQPDSTAPSNSPNLMVFFGTGQYLVDADKT"
     gene            complement(2924..3451)
                     /locus_tag="VIW27_13730"
     CDS             complement(2924..3451)
                     /locus_tag="VIW27_13730"
                     /inference="COORDINATES: protein motif:HMM:NF025061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY5740783.1"
                     /translation="MNNRIYGNQRGMVLVVSMIILISLTVLGLATMQATRTEISMAGN
                     QRELSITFNAAEAGLSAAEQYIDNSISKTIYNDPTQGLYDRLDIDPDYRDPTIWTASQ
                     TATIGLPHVAEEPRFIIKYLGDRSQNEVAAVNIGGYGSSQPGITVSNFRITTRAVGQT
                     GRAIRQLQAYYGKSF"
     gene            complement(3448..4482)
                     /locus_tag="VIW27_13735"
     CDS             complement(3448..4482)
                     /locus_tag="VIW27_13735"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY5740784.1"
                     /translation="MKIKQASRQTGISMVEVLVALVISLFLLSGIVQVYLGNKTAYRF
                     TEAVARIQENGRFAIESITQDLRMAGFFGCAMFDPEDTSNIVNNLNPSGAGYDAALHD
                     FVLAGIINGTEGDGLNFSDSITLKGSKAGQLNIIPPFNVNTSADIFVTSNSIVKPDDI
                     VMVSNCRGADIFQVTNATISSTANKMAVIHNTGAGSPGNYNPDNCQGGNAHCLSQSYG
                     ADAALFRLQSVSYAIAAGASGEPALWRSENGVSLELVEGVEEMQMLYGVDTDDDDFAN
                     QFVTIDQVADTFDIVSVRVMLLLRSTDDFVAEENQTYSFNGNLVNAADTRLRQEFTTT
                     IALRNRVGAS"
     gene            complement(4479..4955)
                     /gene="pilV"
                     /locus_tag="VIW27_13740"
     CDS             complement(4479..4955)
                     /gene="pilV"
                     /locus_tag="VIW27_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY5740785.1"
                     /translation="MHLKTSSQQGLTMVESLVALVVISIGLLGIAGLQLTSMKQNSSA
                     LQHSKAVWASYNMADRIRSNGIRFADYSGIDTDTPYSQDCMSSACSDSQMVTADAAEW
                     TDKVRELPGGRGLISGDASRLTVTVMWDDEGTGATGTDCSGNAQIDLTCFSITLVQ"
     gene            complement(4946..>5426)
                     /locus_tag="VIW27_13745"
     CDS             complement(4946..>5426)
                     /locus_tag="VIW27_13745"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY5740786.1"
                     /translation="ELFITVALISIVTVIGVPSMSNFVNNDRLTTQINSLVGHLALAR
                     SQAITLQTPVTVCASSNQQSCSSADWSTGWVVFVDADASGDFSLGDELLRAHEALEGT
                     SQLSASLGARVVFDDRGFSPTTAGSFSLCDERGVEYLKSISISQTGRVRQGGSASCI"
BASE COUNT          947 a         1546 c         1780 g         1153 t
ORIGIN      
        1 gtcttgtcgg catcgaccag gtactggccg gtgccgaaaa ataccatcag gttcggcgaa
       61 ttgctcggcg cggtcgaatc cggctgcgtc ggatgcctgg cgagtaccgg cttggcggta
      121 atcggctggc ccaccggtgc cttgaacagc ggtctcggcg tcgcattgga gccgtaggcg
      181 aagccccact ggctggggtt gctggcggaa atgtcgaata cccacatgtt gccctgcagg
      241 tcgccggcgt agacccggtc gacggtgccg ttgccgtcga tatcggcgag cgcgggcgtt
      301 gccagcccgt tgcggttggt gggatcaccg acgccggtgg ggattttcca gtagtcgccg
      361 accgtccagt tgccgtcgac gccaccctcg agatcgacga tgtacaggtg ggcctcgccg
      421 ctgccgagat cgttgtagcc gttgccgaag atcgccaccc atttaccgtt attggcgagg
      481 ccgatgaacg gacggctgaa ggtgtagccg agatcgctgt catcgtcgct ggtgaattcc
      541 cacatgacga tcttgtcggc gttggcctcg ctgttgaaca atgaaggctc ggtcacgtcg
      601 agcgcgaaca ggccgcgacc gccgctgcgc aagccgtcca ccagcacggt tcgccattgg
      661 gtgataccac cggtggaaat aaaggcatcg gacacggccg gagtctggtc gacgtaaaac
      721 cggtgcacgt agttggattc ggtcaggtaa tgcagaccct ggccgatcgc gttcgagttg
      781 aaaatgcccg gtacataggc gagaacctcg tcaccgctgg catcgtcgaa tgcatgcaac
      841 atgccgtcgt tggcgccgac gtaaactatt ttcttgcggt ccttcgcagt gccgttcttg
      901 aacttggagt aggcattgag gccctcggga aacggtgcga catccggcca gttgatcgtc
      961 ggctcgccaa cgaataccgg ccccgaattg acgatgtcgc cgagcagtga caggcgatcg
     1021 cgaaagaaaa agccggtacc ctcgttgctt tgatcgccgc gcaggtagtc gagtcgcgcg
     1081 cggccgatcg tgtcgctatc gacgccaccg agcgcattgg tcttcagatc gtcacgcatg
     1141 ccgatcgaga tatcggccca ctggaacggt accccgtccc tgaccgcggg cgcgtcgcta
     1201 taagagatga tggtgcgcgg cttgctgacc aggtcgcggc cgttgaggcg cgatgccgcg
     1261 tcccacttgg gagtatttgc cagcgcgccg gtgtcgaggt cgacgatcgg gtaggccagc
     1321 agattgccct gccagcggtt ggagttgaat tgcgccaggt agactactgc ctcggtgctc
     1381 aggcgcgcgg tattgagggc gaccgcggcg gtggtcccgg tgcgctgcga gatatcggtg
     1441 atcgccgccg acagtgcctc ctgtaattcg atcgggttct gcgcgctgag gtaggtgccg
     1501 cggccgttgt aggatgcgtg ccacaggtcg tcgacggtag tgttcgagtt tttcgacggc
     1561 gccggccagc tgaagccggc tgccagcgga tcggtggtcg ccggatcgag cgtaccgttg
     1621 atgccgaaac cgacgctgta ggttaccagg tgctggtgat cagcctcgtc gatggtggcc
     1681 tgctgcggaa cccgattcgc cagatcggta cgcaggtcgg tctcgtaatt gcgcatcgcc
     1741 acgtcggcca gcgtgttgct ggcgccgtcg gcatagttgc cgccgtcatt ggattgcgcg
     1801 gcattgccgt cgaacgcggt gtcgctggtg ccgccgtcgg cgtcggtatt gccaaccgcc
     1861 ggattggcat tgccgttcca gaaaccgtcg gacatcacga tattgaagtt ttgctggcat
     1921 tcgccgccct cggacgccgg caggatcgga ccggtaccgg tcgcattcat gaagtaatcg
     1981 ccggccgcct tcagtgcgct gcgcgtcggg ctgccgaaga tctggttctg caggttataa
     2041 agcgctgtct gcaggctgcg cttgttgctg gcgtctttca tcgtcgccac gtcggcgata
     2101 tggccggcat tgaaccagct ggcgcccatg cgcgaggcgt cggtgctgtt gatggtgtcg
     2161 ccgacgattg ccttggtggc gtgcatgcgc gtacggtaat actggaacca gttggcgaaa
     2221 ttctgcatct ccgtggtatt gggggcgatc tcgattcgcg tgttggcatc ggttacctcg
     2281 agtacgccgt cgttgtcgct gtcgatccag acgtgatagg tcggaatcca gtagttggta
     2341 acggtggcgg cgtcttcggt gaagctgtga ttgatggtca ggttgacgaa ctcgccgagc
     2401 ggctggccgg ggtgcttcag cgctttgctc ggattggcgt cggcgtagat cggcgaaccg
     2461 tcgggattgg tacccgccca gggtttgtaa gtgacactcg ggttgtaata gttggggttc
     2521 gccaggtggt tgcggactac ccacgaccgg tcccagacgc tgttcgcgcc gttgctcggc
     2581 ggcagcacgt aaactccgtc agcattggta taaagatggc tgaagctcgg cgtgtaatag
     2641 gcgcggtaac ggccgttgat gatcggcagg ccggtggcgg tgccgaggcc ggtgtcggag
     2701 cgcaatatcg ggccccagtc catgctgccg gaatcgtcga gggtaaagta aacgttcgat
     2761 tccaccagtg tcgacaggaa cagcggcgcc ggcggcaggt tacccggcgc gccgtgggcc
     2821 tggtacagcg gcatcgccgt cagcgccgcg gtcgccagga tcctggccag gtatttgcgc
     2881 atataatcgt tcatctgaat cctcctgatc cctgaccgcc ttcctagaaa gacttgccgt
     2941 agtaggcctg tagctggcgg attgcccggc cggtctgtcc gacggcgcgg gtggtaatgc
     3001 ggaaattcga taccgtgatg cccggctgac tgctgccgta gccgccgata ttcaccgccg
     3061 ctacctcgtt ctgcgaacgg tcgccgagat acttgatgat gaagcgcggc tcctcggcga
     3121 cgtgcggcag gccgatggtg gcggtctgcg aagcggtcca gattgtcgga tcacgataat
     3181 cgggatcgat atccagccga tcgtagaggc cctgggtcgg gtcgttatag atggttttcg
     3241 atatcgagtt gtcgatgtac tgctcggcgg cactcagtcc cgcttcggcg gcgttgaagg
     3301 tgatgctgag ttcacgctga ttgccggcca tgctgatttc ggtgcgcgtg gcctgcatgg
     3361 tagcgagccc gagcacggtc agcgagatta gtattatcat gctgacgacc agcaccatgc
     3421 cgcgctggtt gccatagatt cgattgttca tgatgccccc acccggttgc gtagagcgat
     3481 ggttgtcgtg aattcctggc gcaaccgtgt atcggcggcg ttcaccaggt tgccgttgaa
     3541 gctgtaggtc tgattctcct cggcgacgaa atcgtccgtg gagcgcagca acagcatgac
     3601 gcgcaccgag acgatgtcga aggtgtcggc gacctggtcg atggtgacga actgattggc
     3661 gaagtcgtcg tcgtcggtat ccacgccgta gagcatctgc atttcctcga caccctcgac
     3721 cagctcgagg ctgaccccgt tttcggagcg ccacagggcg ggttcgccgc tggcaccggc
     3781 cgcgattgcg taggatacgc tctgcaggcg aaacagggcc gcgtcggcgc cgtaggattg
     3841 cgacaggcaa tgtgcgttgc ctccctgaca gttgtccggg ttgtagttgc ccgggctgcc
     3901 ggcgccggtg ttgtgaatta ccgccatctt gttggcggta ctgctaattg tagcgttggt
     3961 gacctgaaag atgtccgcgc cacggcaatt gctgaccatg acgatatcgt cgggtttgac
     4021 gatgctgttg gaggtgacga agatgtcggc cgaggtgttg acgttgaagg gcggtatgat
     4081 gttcagctgg ccggccttcg agcccttcag tgtaatactg tcggaaaagt tgagaccgtc
     4141 gccctcggtg ccgttgatga tcccggcgag cacgaaatcg tgcagcgccg cgtcatagcc
     4201 cgcgccgctc ggattgaggt tgttgacaat attgctggta tcctcaggat cgaacatcgc
     4261 gcaaccgaag aatccagcca tgcgcaggtc ctgggtgatg ctctcgatcg cgaaacggcc
     4321 attttcctgg atgcgggcaa ccgcctcggt gaagcggtac gccgtcttgt tgccgagata
     4381 aacctgcacg attccactga gcaggaacag gctgatgacc agcgcgacga ggacttctac
     4441 catcgagatg ccggtttgcc ggcttgcttg cttgattttc attgcaccag ggttatcgaa
     4501 aaacaggtta aatcgatctg cgcgttgccg ctgcagtcgg tgccggtcgc gccggtacct
     4561 tcgtcgtccc acatcacggt gaccgtcagc cggcttgcat cgccgctgat cagcccgcgt
     4621 ccgccgggaa gctcgcgcac cttgtcggtc cactcggccg cgtccgcggt gaccatctgc
     4681 gaatcgctgc aggcggaact catgcaatcc tgcgagtagg gtgtatcggt gtcgatgccc
     4741 gaataatcgg cgaagcgaat gccgttggag cgaatacggt cggccatgtt gtaactggcc
     4801 cagactgcct tgctgtgctg cagggcgctg ctgttttgct tcatcgaggt cagttgcagc
     4861 ccggcaatgc cgagcaggcc gatcgaaatg accaccagcg ccaccaggct ttcgaccatc
     4921 gtcaggcctt gctgggaaga cgtcttcaga tgcatgatgc gcttcctccc tgtcttacgc
     4981 ggccggtttg cgagatcgaa atcgacttca agtactcgac gccgcgctcg tcgcacagcg
     5041 aaaaactacc ggcggtggtg ggtgagaagc cgcggtcatc gaaaaccacc cgcgcgccga
     5101 ggctggccga caactggctg gtaccctcga gggcctcgtg agcgcgcagc aattcgtcgc
     5161 ctagcgaaaa gtcgccgctg gcatcggcgt cgacgaatac cacccagcca gtcgaccagt
     5221 ctgcggacga gcagctctgt tggttgctgc tcgcgcatac ggttaccggc gtctgcaggg
     5281 taatcgcctg gctgcgcgcc agcgccagat gaccgaccag cgaattgatc tgcgtcgtca
     5341 gccggtcgtt attgacgaag ttcgacatcg acggtacgcc gatgacggtg acgatcgaga
     5401 tcagggcgac ggtgataaag agctcg
//
LOCUS       DATCZV010000611         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_261731, whole genome shotgun sequence.
ACCESSION   DATCZV010000611 DATCZV010000000
VERSION     DATCZV010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261731"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VIW27_13750"
     CDS             complement(<1..1210)
                     /locus_tag="VIW27_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEY5740787.1"
                     /translation="MFDRLKKTHITAAPGFNRWRVPPASIAIHLCIGSVYAWSIYNPA
                     LVRAQGVVASAATDWSLKDVVWIFTVSIVFLGLAAAFAGRWLERVGPRMVGVVAACCW
                     GGGYLVGSLGIVTHQLWLLYLGYGVIGGCGLGLGYVSPVSTLIRWFPDRRGMATGMAI
                     MGFGGGAMIGAPLKGYLIRTFYRAPEYLGTVDDVNLVTEAGKRFADVAGSLVEVVVVG
                     ANDVANMILPGPEGVYVVGTGSTGVAETFVALGVLYFVVMIIAAFAYRLPAEGWKPEG
                     WTPPDPAHAAKRMITDRDVDIDQALRTRQFWQLWIVLCFNVTAGIGVLGVARTMITEI
                     FGSTLPTIVDGAFAATYVVMISVFNMIGRFFWASTSDYIGRRNTYWIFFGLGIVLYCS
                     IPYSAQQVSAD"
     gene            complement(1242..2402)
                     /gene="hutI"
                     /locus_tag="VIW27_13755"
     CDS             complement(1242..2402)
                     /gene="hutI"
                     /locus_tag="VIW27_13755"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY5740788.1"
                     /translation="MAGGRYGIVEDAAVGISAGRIAWLGPAADLPPHQAAQSTSLAGR
                     WLTPALIDCHTHLVFAGNRADEFEQRLKGASYEDIARAGGGIMSTVNATRAADHAELL
                     AQSVKRLRQLRAEGCATVEVKSGYGLDVETEIRMLEVARSLAAETGIDIVTTFLGAHT
                     VPAEFRGNEDAYVDLVCDEMLPAVHERGLADAVDAYCEPIAFSADQVARVFETARSLG
                     LPVKLHADQLSDSGGAALAASFQAVSADHLEYAAATGIDAMAAAGTAAVMLPGAFLTL
                     GETQLPPIDALRAGEVPIAVATDCNPGTSPVCSIRTTMTLAARLFGLTPEECLAGTTI
                     HAARALGLDDRGSIEVGKRADLAAWDIGHPQELSYWIGTNELTELYVNGRRL"
     gene            2525..>3270
                     /locus_tag="VIW27_13760"
     CDS             2525..>3270
                     /locus_tag="VIW27_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5740789.1"
                     /translation="MITIFARLALTSDGWAENVRIAVDGRLIGSVDADSPAVDGDVEA
                     GIVIPGLANAHSHAFQRALAGHTEHRGPGNKDNFWTWRSRMYALAGRIDAAALEVIAR
                     QAYTEMVATGFTAVAEFHYLHNEPGESGPSEAMFEALANAAACSGIRLTYVPVLYERA
                     GFDNPAPSGEQRRFVRQCDEFIEHYEKVTAACRDGVRTGIGVHSLRAVSAQSLRKVAA
                     IAARDEAPMHLHIAEQVKESDDCLAALGMQP"
BASE COUNT          581 a         1078 c         1076 g          535 t
ORIGIN      
        1 gatcggcgct gacctgctgc gcgctgtacg ggatggagca gtacaggacg atgccgaggc
       61 cgaagaatat ccagtaggta ttgcgccgcc cgatatagtc cgacgtgctg gcccagaaga
      121 atcggccgat catgttgaat acgctgatca tgacgacata ggtcgcggca aaggcgccgt
      181 cgacgatcgt cggcagggtg ctgccgaaaa tctcggtgat catcgtcctg gccacgccga
      241 gtacgccgat gccggcggtc acgttgaaac acagcacgat ccagagctgc cagaactggc
      301 gcgtccgcaa tgcctggtca atgtcgacgt cgcggtcggt gatcatgcgc ttcgcggcat
      361 gcgccgggtc aggcggcgtc catccctcgg gtttccagcc ctccgcgggc agtcgatagg
      421 cgaacgccgc gattatcatg acgacgaaat acagcacgcc gagcgcgacg aaggtctcgg
      481 caacgccggt cgatccggtg ccgacgacgt acacgccttc cggacccggc aggatcatgt
      541 tggcgacgtc gttcgcaccg acgaccacca cttcgaccag gctgccggcg acatcggcga
      601 aacgcttccc ggcctcggtt accaggttga cgtcgtccac ggtgccgagg tattccggcg
      661 cgcggtagaa ggtccggatc aggtatccct tgagcggtgc gccgatcatt gcgccgccgc
      721 cgaatcccat gatcgccatc ccggtcgcca tgccgcggcg atccgggaac cagcggatca
      781 gtgtgctgac cggcgagacg tagccgaggc cgagtccgca gccgccgata acgccgtagc
      841 ccaggtacag gagccagagc tggtgggtga cgatgccgag gctgcctacc aggtagccgc
      901 cgccccagca gcacgccgca acgacgccga ccatccgcgg gccgactcgt tcgagccagc
      961 gacctgcaaa cgccgccgcc agtccgagga agacgatgga caccgtgaat atccagacga
     1021 cgtccttcag cgaccagtcg gtggccgcgc tcgcgacgac accctgcgcc ctgaccagcg
     1081 ccggattgta gatgctccag gcgtagaccg agccgatgca gaggtggatg gcgatggatg
     1141 ccggcgggac ccgccagcga ttgaaaccgg gcgcggcggt aatgtgcgtt ttcttcagcc
     1201 ggtcgaacat gcgccccccg cgtgactatc agaggcctgt tctacaggcg gcggccgttg
     1261 acgtaaagtt cggtcagctc gttcgtgccg atccagtacg acagttcctg cggatggccg
     1321 atgtcccacg cggcgaggtc ggcgcgcttg ccgacttcga tcgagccacg atcgtcgagc
     1381 cccagcgccc gcgctgcgtg gatggtcgtg cccgcgaggc actcttccgg cgtcagcccg
     1441 aacaatcgcg ctgccagggt cattgtcgtc ctgatcgagc agaccggcga ggttcccgga
     1501 ttgcagtcgg tcgcgaccgc gatcggcacc tcgccggcac gcagcgcgtc gatgggcggt
     1561 agctgcgttt ccccgagcgt caggaaggcg cccggcaaca tgaccgccgc ggtccccgct
     1621 gccgccatcg cgtcgatgcc cgtcgctgcc gcatattcca ggtgatccgc cgaaaccgcc
     1681 tggaaactcg cggccagtgc agcgccgccg gagtcgctca attgatccgc gtgcagcttg
     1741 accggcaggc cgagcgacct cgctgtctcg aacacccggg caacctggtc agccgagaac
     1801 gcgatgggtt cgcaataggc gtcgacggcg tcggcgagtc ctctttcgtg cacggcgggc
     1861 agcatctcgt cgcacaccag gtcgacgtag gcgtcctcgt tgccgcggaa ttccgccggc
     1921 accgtgtgcg cacctagaaa tgtcgtcacg atatcgatgc cggtttcggc ggcgagcgat
     1981 cgggcgacct cgagcatgcg aatctcggtt tcgacatcaa gaccgtagcc ggacttgacc
     2041 tcgacggtcg cgcaaccttc ggcccggagc tgtcgcaatc gcttcacgga ctgcgcgagc
     2101 agttcggcgt ggtcggcggc gcgtgtcgca ttgaccgtcg acatgatgcc gccgccggcg
     2161 cgggcgatat cctcgtagga ggcgcccttc agtcgctgtt cgaattcgtc ggcacggtta
     2221 ccggcgaaga cgaggtgggt atggcagtcg atcagggcgg gcgtgagcca gcggccggcc
     2281 agcgatgtcg attgcgcggc ctggtggggc ggcagatcag cggcggggcc gagccaggca
     2341 atcctgccgg cactgatgcc gaccgctgcg tcctcgacga tgccgtaacg gccgccggcc
     2401 atcgtcgcca cccgggcgcc ggtaatcagg agatcgaacg actgcatgcg gtcctcggac
     2461 gcggcaattg caatgcaccg tagcatgcac tagcctgtat atacaacctc ctgaagggcc
     2521 cgtaatgata acgatttttg ccaggctggc gctgacctcc gacggctggg ccgagaacgt
     2581 ccgcatcgct gttgacggcc ggctgatcgg cagcgtggac gccgacagcc ctgccgtcga
     2641 cggcgacgtc gaggccggta tcgtcattcc aggcctcgcg aacgcgcaca gccatgcttt
     2701 ccagcgggcc cttgccggcc acacggaaca ccgcggtccc ggtaacaagg acaacttctg
     2761 gacctggcga tcgcggatgt acgcgctggc cggccgcatc gatgcggcgg cgctcgaagt
     2821 gatcgctcgc caggcataca cggagatggt ggcgaccggc ttcacggccg tcgcggaatt
     2881 tcattacctg cataacgagc ccggcgagtc gggcccctcc gaagcgatgt tcgaagcgct
     2941 tgcgaatgcc gcggcctgca gcggcatccg cctgacctat gtgccggtcc tttatgaacg
     3001 cgccggcttc gacaacccgg cgccgagcgg tgagcagcgg cgcttcgtca ggcaatgcga
     3061 cgagttcatc gagcactacg aaaaagtgac ggcagcctgc cgcgacggcg tccggaccgg
     3121 catcggcgta cacagcctgc gcgcggtttc tgcgcagtcg ttgcgaaaag tcgccgcaat
     3181 tgctgcccgc gacgaggcgc cgatgcacct gcatatcgcc gagcaagtga aggaatccga
     3241 cgattgtctt gcggcgctcg gcatgcagcc
//
LOCUS       DATCZV010000612         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_102096, whole genome shotgun sequence.
ACCESSION   DATCZV010000612 DATCZV010000000
VERSION     DATCZV010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102096"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2397)
                     /locus_tag="VIW27_13765"
     CDS             complement(<1..2397)
                     /locus_tag="VIW27_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5740790.1"
                     /translation="MAAGFTADDPNKLADALLTLIEDLVCELRADNTAGIMLSLDSSL
                     DDDLGLDSLARVELVSRIESHFNVSLKLRDFAEAESPRDLLRSIIDAQGQKKSCPGRQ
                     VIERAVDAAVEPPRHAATLVEVLEWHVQHSPERLHINILGDEEEAQGLSYRQLWQGAR
                     QTAAGLQQRGIQPGETVAIMLPTGRDYFFSFFAILLSGAIPVPIYPPVRRSQLEDHLL
                     RHGSILDNCAASLLITMPEAKVVARLLKSRVLSLREVVTLKDLSSVAATYLRPAIKPG
                     DIAFLQYTSGSTGNPKGVVLTHENLLANIRAMGEAVAAKPDDVFVSWLPLYHDMGLIG
                     AWLGSMYFAMLLVVMSPISFLSRPERWLWAIHRYRGSLSAAPNFGFEFCRLRLDDDAI
                     AGLDLSSWRLAFNGAEPVSADSIEGFIHRFEAYGFRAEAMMPVYGLAESSVGLAFPPL
                     NRKPLIDRVERDAFSRCGQAIPAATGDDKALQFVACGHALRGHQIRIVDSEGQQLPER
                     QQGSLLFRGPSATSGYYRNPEATRSLFVEGWLDSGDLAYIAEGDVFITGRNKDMIIRA
                     GRNIDPPELEEAVGKIDGIRRGCVVAFAARDQRYSTERLVILAESRATDQQTRQALKN
                     RIGEISSDLIGLPADEVIIAPPHTVLKTSSGKVRRSACRQLYEQNKLGQAPRSLWLQV
                     ARTVAASAIPLGRRLLHDLGGFAFAVYTWTLFMALAAAAWAGVILLPTLQWRWRATRG
                     LTRLLFGASGIKIVVDGVENLPPESLAAVYVANHSSYLDSIAVVASIPRNFSFVAKPE
                     L"
BASE COUNT          517 a          764 c          739 g          518 t
ORIGIN      
        1 gagctctggc ttggcgacaa aactgaaatt gcgcggaatc gaggcgacca ccgcgatgct
       61 gtccaggtag ctgctgtgat tggcgacata aaccgcggcc agcgattcgg gaggcagatt
      121 ttcgactccg tcgacgacaa tcttgattcc cgaggcacca aacagtaatc gcgtcaggcc
      181 acgcgtcgct cgccagcgcc attgcagggt cggtaacagg ataacgcctg cccaggcggc
      241 ggcggccaac gccataaaca gtgtccatgt ataaaccgca aaggcgaagc cgccgagatc
      301 gtgcagcagt cggcggccca gcgggatcgc cgatgctgcc actgttcttg ctacctgcag
      361 ccacaacgac cggggggcct ggccaagctt gttttgttca tagagttggc gacaggccga
      421 gcgcctgacc ttgccgctgg atgtcttcag caccgtatgc ggcggtgcga taatcacttc
      481 atccgcgggt aaaccgatca agtcactgga tatttcgccg attcggtttt tcaatgcctg
      541 tcgggtttgc tgatcggttg cccgggattc cgccagtatc accaggcgct ccgtcgaata
      601 acgctggtct cgcgcggcaa aagccacgac acagccccgg cgaataccgt ctatcttgcc
      661 gacggcttcc tcgagttcgg gcgggtctat attgcggccc gcgcgaatga tcatgtcctt
      721 gtttctgccg gtaatgaata cgtcaccctc ggcgatatag gcgagatcgc cggaatccag
      781 ccagccttcg acgaacagcg atcgcgtcgc ttcgggattg cggtaataac ccgaggtcgc
      841 cgacggaccg cggaacagca ggctgccctg ttggcgctcg ggtaactgct ggccctcgct
      901 gtcgacgatg cgaatctggt gtccgcgcaa cgcgtgccca caggcgacga attgcagcgc
      961 cttgtcatcc cctgttgcgg caggtatggc ctgaccgcat cgactgaatg catcgcgctc
     1021 gacgcggtcg atcagcggtt ttcgattcaa cggcggaaaa gccaggccga ccgaagattc
     1081 ggccaggcca tacaccggca tcatcgcctc ggccctgaaa ccgtatgcct cgaaacgatg
     1141 gataaagccc tcgatcgagt cggcactcac cggttccgcg ccattgaagg ccagccgcca
     1201 ggaactcaaa tcgaggcccg cgattgcgtc gtcgtcgaga cgcaaccggc aaaattcgaa
     1261 accgaaattc ggcgctgccg acaggctgcc ccgatatcga tgaattgccc ataaccagcg
     1321 ttcgggcctg gacaaaaagc tgattggcga catgaccacc agtaacattg caaaatacat
     1381 gctgcccagc caggcgccaa tcaaacccat gtcgtggtag agcggcagcc agctgacaaa
     1441 gacgtcatcg ggcttcgccg ccaccgcctc gcccatcgcg cgaatattcg ccagtagatt
     1501 ttcatgcgtc agaaccacgc ccttgggatt gccggtactg ccggaggtat attgtaaaaa
     1561 agcgatatcg ccaggcttga tcgccgggcg cagataggtt gccgccaccg aactcagatc
     1621 cttgagcgtg accacttcgc gcaagctcag tacacgggat ttcagcaagc gggcaaccac
     1681 ctttgcctcg ggcatggtaa tcaacaggct cgcggcacag ttgtcgagaa tactgccgtg
     1741 cctgagcagg tgatcttcga gctgactgcg tcttaccggc ggatagatag gcaccggaat
     1801 cgctccgctt aagaggatag cgaaaaagct gaagaagtag tcgcgtccgg ttggcagcat
     1861 gatggccacc gtttcgcccg gctggatccc gcgttgttgc aggccggcgg cagtttgccg
     1921 tgcgccttgc catagctgcc ggtagctcag gccctgcgcc tcttcctcgt caccgaggat
     1981 gttgatgtgc aggcgctcgg ggctgtgctg tacatgccac tcgagcacct cgaccagcgt
     2041 cgccgcgtga cggggcggtt ccacggcagc gtcgaccgcg cgttcgatta cctgtctgcc
     2101 ggggcaggac tttttttgtc cctgggcatc gatgatggag cgcagcaagt cacgcggcga
     2161 ttcggcctcg gcaaagtcgc gcagcttcag gctgacgttg aaatggcttt cgatacgcga
     2221 aaccagctcg acacgtgcca ggctgtcgag cccgagatca tcgtcgagcg agctgtccag
     2281 actgagcatg atgcccgcag tgttgtcggc acgcagttcg cagaccaggt cttcgatcag
     2341 cgtcagcagg gcgtcagcca gtttgttcgg gtcgtctgct gtaaaaccgg ctgccatacc
     2401 aattccgctg aattatcgag cctcgtttaa ctgcctgcct tgcacacgat attttttacc
     2461 cgggtaaacg ccattgactt gtctcaatta gtggctgatt ttttgtcctg ctgttgcggg
     2521 ctttatcgag cgtcacat
//
LOCUS       DATCZV010000613         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_420155, whole genome shotgun sequence.
ACCESSION   DATCZV010000613 DATCZV010000000
VERSION     DATCZV010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420155"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VIW27_13770"
     CDS             <1..417
                     /locus_tag="VIW27_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton symporter"
                     /protein_id="HEY5740791.1"
                     /translation="LRRIVPAERRAALRREVDGLTVLFLVVIAIAIMDGVSARILEDP
                     AKAMTIVLAAFAVSATLHAVGGTLFWRMGATAGLAMAVTSGSRNLILLLVVLGEAAGD
                     TFTLYVALGQLPIYLMPTAIQPICRVLLRRANRRAA"
     gene            complement(388..>2196)
                     /locus_tag="VIW27_13775"
     CDS             complement(388..>2196)
                     /locus_tag="VIW27_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY5740792.1"
                     /translation="ASSVKDRKKGTTEDLAWRDGTVEERLSHALVKGINEFIVADTEE
                     ARLTRERPIEVIEGPLMDGMDIVGELFGSGQMFLPQVVKSARVMKQAVAHLIPFIEAE
                     KGGKAKAKGKVLMATVKGDVHDIGKNIVGVVLQCNNFEVIDLGVMVPYTKILETARAE
                     KVDMIGLSGLITPSLEEMAIVAAEMTREGFDIPLLIGGATTSKVHTAVKIAPNYSGPI
                     VHVVDASRAVGVAGNLVSETHNSPFTQQIQAEYDDIRTRHAGQRSGAKQATLEAAREA
                     AHRIDWSQVAPPKPDFLGLKTFERYDLAELVESIDWTPFFRTWELAGVYPALLEDSVV
                     GDAARSLFQDAKAMIERVVAEEWLGANAVIGFFPANAVGDDVEIYTDDSRSTVHRTFH
                     FLRQQMSKRAGGTNYCLADFVAPKESGVADYIGAFAVTAGLGIEERLRAFKSDHDDYN
                     DILLKAVADRFAEAFAERMHQRVRMEFWGYAPEEDLSNQDLIKERYQGIRPAPGYPAC
                     PDHTEKSDLFDLLQAEKNASISLTENFAMLPTAAVSGFYFWHPESRYFGIGKIGRDQV
                     EDYARRKGVPLLQAERWLASNLSYDRASGGAPVRAA"
BASE COUNT          377 a          716 c          651 g          452 t
ORIGIN      
        1 ctccgccgta tcgttccggc agagcggcgc gccgcccttc ggcgcgaggt tgacggcctg
       61 accgttctgt ttctggtcgt tatcgcaatc gccattatgg atggcgtgag cgcccggatc
      121 cttgaggatc ccgcgaaggc aatgacaatc gtgctcgccg ctttcgcggt cagcgcgacg
      181 ctgcatgcgg tcggcggcac gctcttctgg cggatgggcg caaccgcggg attggccatg
      241 gcggtgacca gcggcagcag gaatctgatt ttgctgttgg tggtgctggg cgaggcggcc
      301 ggcgatacct tcacgctcta tgtcgcgctc ggccaattgc cgatctatct catgccgacg
      361 gccattcagc cgatctgccg cgtgttgtta cgccgcgcga accggcgcgc cgcctgacgc
      421 ccgatcgtag gagaggtttg acgcaagcca gcgctccgcc tgcagcagcg gcacgccctt
      481 gcgccgcgcg taatcctcca cctgatcgcg gccgatcttg ccgatgccga agtagcgcga
      541 ctccggatgc caaaaataga acccggaaac ggccgccgtc gggagcatcg cgaaattctc
      601 ggtcagcgat atcgatgcgt ttttttccgc ctgcaagaga tcgaacagat cggatttttc
      661 cgtatggtcc ggacaggcag gatagcccgg cgcgggccgg atcccctgat agcgctcctt
      721 gatcaggtct tgattggaca agtcctcctc cggcgcatag ccccagaact ccatgcgcac
      781 ccgctgatgc atccgttccg caaaggcttc cgcgaaccgg tcggcaaccg ccttgagcag
      841 gatatcgttg taatcgtcat gatcggattt gaatgcgcgg agacgctctt cgattccgag
      901 cccggcggtc accgcgaagg cgccgatgta atcggccacc cccgactcct tcggcgcgac
      961 gaaatcggca agacagtaat tggtgccgcc cgcgcgtttc gacatctgct ggcgcaggaa
     1021 atggaaggtc ctgtgcaccg tggaacggct gtcatccgta tatatttcga catcgtcgcc
     1081 gaccgcattc gcggggaaaa atccgattac cgcgttggcc ccgagccact cttcggcgac
     1141 gacccgctcg atcatcgcct tggcgtcctg gaacaacgat cgcgcggcgt cgccgaccac
     1201 cgaatcctcg agcagtgccg gatacacgcc ggcgagttcc caggtgcgga aaaacggcgt
     1261 ccaatcgatg ctctcgacga gctcggcaag gtcgtatcgc tcaaacgtct tgagcccgag
     1321 aaagtcgggc ttcggcggcg ccacctgcga ccaatcgatg cggtgggcag cctcgcgcgc
     1381 ggcttccagc gtcgcctgtt tggcgccgct gcgttgaccg gcatggcggg tgcgaatgtc
     1441 gtcgtactcc gcctgtatct gctgggtaaa cggcgaattg tgggtctcgc tcaccagatt
     1501 gccggcaacc ccgaccgctc gcgaggcgtc gaccacatga acgatgggac cgctgtaatt
     1561 gggagcgatt ttcaccgccg tgtgcacctt ggaggtcgtg gccccgccga tcagcagggg
     1621 gatatcgaag ccttcgcgcg tcatttccgc agcgacgatc gccatttcct cgagcgacgg
     1681 cgtgatcaaa ccgctcaacc cgatcatgtc gactttttcg gcccgggcgg tttccaggat
     1741 cttggtgtag ggcaccatca cgcccagatc gatcacctcg aagttgttgc actgcaggac
     1801 cacgccgacg atatttttgc cgatgtcgtg cacgtcgccc ttgacggtgg ccatcagcac
     1861 cttgcccttg gctttggctt ttccgccttt ttccgcctcg atgaagggga tgagatgggc
     1921 caccgcctgc ttcatgaccc gggcgctttt caccacctgc ggcaggaaca tctgccccga
     1981 tccgaacagc tcgccgacga tatccattcc atccatcaac ggcccctcga tcacctcgat
     2041 cggccgctcg cgcgtcagcc gcgcctcctc ggtatcggcg acgatgaatt cgttgatccc
     2101 cttgaccagc gcatggctca atctttcctc gacggtgccg tcgcgccagg cgaggtcttc
     2161 cgtcgtgcct tttttgcgat cctttaccga actggc
//
LOCUS       DATCZV010000614         9276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_526133, whole genome shotgun sequence.
ACCESSION   DATCZV010000614 DATCZV010000000
VERSION     DATCZV010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526133"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VIW27_13780"
     CDS             complement(<1..545)
                     /locus_tag="VIW27_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5740793.1"
                     /translation="MKTLNSEARTCGEWLVELLEDCGITTVFGIPGVHTVEFYRGLEK
                     TSLRHITPRHEQGAGFMADGYARASGMPAACFIISGPGMTNILTAMAQAYSDSVPMLV
                     ISSCLDVKDAAQGRGRLHEMIDQSGAAASVAQSAATAYTPKDLKDRVAEAFARLASQR
                     PRPCYIEVPIDVLKMPAGEGWT"
     gene            complement(579..1355)
                     /locus_tag="VIW27_13785"
     CDS             complement(579..1355)
                     /locus_tag="VIW27_13785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY5740794.1"
                     /translation="MLSSAARILFVEMMGVPGSYDASVYDHFEDRDQEGLWFVKRYFR
                     VPGIRIATCNVCLGETLPGPGEVDGLVLAGSYNSVHDDTGWQRRMRAWLPEMRRAGVP
                     VLGICGSHQLIAHMEGADVLRVEGGPFAGTFPLELTAAGRASPLMAGIGDGACFHYAN
                     SEHVVGIPAGSTLLGSSSKVPVAALDYGGHCYTTQFHPEASEETLGTIWRFKAPALMR
                     RYHPRDMGDRLVENFLCLARDLCTDRYQAPRGRRLGFEPC"
     gene            complement(1349..3802)
                     /locus_tag="VIW27_13790"
     CDS             complement(1349..3802)
                     /locus_tag="VIW27_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740795.1"
                     /translation="MNANTDLPQEAKVVIIGGGIIGCSTAYHLARMGCSEVVLLEKHR
                     LTSGSTFHAAGLVGQLRTNANITQLLGYSVELYRTLEVETGLATGWKMNGGLRLACNA
                     DRWTEVRRQATTAHSFGLEMHLLTPREALDLWPVMEVADLVGAAFLPTDGQANPSDIT
                     QSLASGARKGGVLIREGVEVTGFEFDGDTLRALETDAGKIRCERAVICAGQWSHRLGR
                     LAGVEIPLVSVQHQYLVTEPIAGVAPDLPTLRDPDRLTYFKEDVGGLVMGGYEPNPIP
                     WARDGIPEGFNFTLLDNDWAHFEPLMEQAIARVPALADAGIRQMFNGPESFTPDGNFI
                     LGEAPERRNLFVGAGFNAFGIAAGGGAGMALAEWVVNGEPPFDLWPVDIRRFGQPHRD
                     VEWVRRRTLEAYSKHYTMAWPFEENRTCRPSRRSPLYETLKAQGACFGEKLGWERPNW
                     FADDPASARDEYGFGRQNWFEAVGREHRAARERAAVFDQTSFAKYRIRGAGAEPALSW
                     LCANDVAVADGAVVYTQLLNRRGGIECDLTVTRVGEDEYYLVTGTGFATHDLDWIRRN
                     LPQGLDVRVDDVTTAWAVLSLMGPKSREILQALVRDDLANEWLPFASMRELAIAGALV
                     RALRVSYVGELGWELHVPVESALSVYEALMAAGAGHGIVNGGYRAIESLRLEKGYRAW
                     GADIGPDYTPLEAGLGWAVDLASGKDFLGRDAIVAQRAGPLHRRLACFTLADRDVVLL
                     GRETIYRDGERVGWLSSAGWGYTAETNIGFGYVRHAEGVDAGFLAAGTYELEVAGERR
                     PCALQVGPLYDPEMHSLKC"
     gene            complement(3804..5810)
                     /locus_tag="VIW27_13795"
     CDS             complement(3804..5810)
                     /locus_tag="VIW27_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5740796.1"
                     /translation="MSHAAASLLKPALPLTGRWTLAWLFAIAATAGLMLAKDALPWAF
                     DYPRAWQLPLASWISAFMKWLINSFDFGLFTFKDLTRWIAWLVEQPYRLARSLLSTGF
                     LSGVGSDAVPVFPRISWLALIAGVMLLGHYVRDWKLALLAGGCFAYLVVFGQWDSAMV
                     TLSSIIIAVPIGVVGGMLLGILGHRSPRFRLLLIPILDLMQTVPVFAYLVPVLLLFGF
                     SPVSAMIATIIYAMPPMVRVTLLALDHIGAEVVEAGTMAGCSRRQLLWKVLLPSAKRS
                     LMVGVNQVVMLSLNMVIIASMIGAGGLGYDVLTALRRLRIGEGVEAGVAITLLAIALD
                     RLSQAYANRPPAMHGRPGESLWQRHPHTLLFVAAVGLTGLLGIYMPALNAYPENWQIT
                     TGPFWGELVRYINVNYFDALESFKNFLLLNLMVPFKRFLLSLPWIAVVAMVGLGGWQL
                     GGRRLGLLTAALAAFIAVVGLWDKAMVTLYLCGLSVVIACCIGIPIGILAARNDRLHR
                     VVGVVIDTLQTLPSFVYLMPVVMLFRVGDFAAMLAVIAYAITPVIRYTDHGIRGISAE
                     LIEASRQVGMTRNQLLRKVQIPLALPHIMLGVNQTIMMALSMLVITALVGTRELGQEV
                     YIALTKADTGRGIVAGVCVAFIAIISDRMISAWASRRKREYGLE"
     gene            complement(5807..6862)
                     /locus_tag="VIW27_13800"
     CDS             complement(5807..6862)
                     /locus_tag="VIW27_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593578.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5740797.1"
                     /translation="MPHAPVQPDETAVKIRCRHVWKVFGEGVPALFAAGGRPDTAVLE
                     QGGYIGAVRDASFDVSEGETFVIMGLSGSGKSTLLRCMTRLIEPSHGEIYFESQDLMK
                     VGEAQLIDLRRHKMGMVFQHFALLPNRTVLGNVALPLEVQGIGRAEREARAIEVIRLV
                     GLEGREHYYPRELSGGQQQRVGIARSLAAEPDIWFLDEPFSALDPLIRREMQDEFMRL
                     QKLLHKTIVFITHDFEEAIRLADRIAIMKDGAIMQIGTPEELVLNPATDYVEEFTQGI
                     PRAKVLTAGALMRAVESDSFAGTVQANLPIEPQLDAILNADRGAVFAVRDDEGNTVGS
                     LPREVVVDLLSRGQANE"
     gene            complement(6959..7912)
                     /locus_tag="VIW27_13805"
     CDS             complement(6959..7912)
                     /locus_tag="VIW27_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740798.1"
                     /translation="MNKKSSSVVLRSILLAACAALAAPAGAVESKDPIKLTLHDWTGQ
                     YLTTTLMGEILKKAGYNVEYVQADYIAQFAGLESGDLHIAMEMWETTGKQAMDESLKT
                     GNTVDLGETGMDAKEEWWYPMYMKEKCPGLPDWKALNACAEAFSIPETAPKGRYLGGP
                     VTWAGFDDERVEALGLDYEVVHAGTDAALFSELESAIQRKAPILLWVYAPHWAVAKYD
                     GEWIEFPEYTDACYEDPSWGINKNMAYDCGKPFGWIKKVGWKAGEKKWPGAYKAVRNF
                     KINNAQMSQMIVEVDLEGKSVDETVAKWMRDNESTWKAWIQ"
     gene            complement(8033..8965)
                     /locus_tag="VIW27_13810"
     CDS             complement(8033..8965)
                     /locus_tag="VIW27_13810"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEY5740799.1"
                     /translation="MNDDLQQVHRSLTSIPGFENLSADAYTTQRLGGLTNLVYRIDTG
                     AQRLVLRIPGKGTEEYIDRAVEMHNARVAAKAGVSAEVVYADAQSGVMLTRHVDGIVT
                     MSPAAFADREGAPGRAGRAFRRMHDWSEPFEFRFELFSMIDGYLKILDERKAELPEGY
                     AEVVDAAQPVREALDRHPAKLAPCHCDPLCENFLDSGERMWIVDWEYSGMNDPLWDLG
                     DLSVEAGFTPAQDEELIRAWCGGEPGADEFGRMIIYKAMCDLLWTLWGLIQHSDGNPA
                     EDFWAYSIGRFERCKTLMSAAGFERHVKAVAAGS"
     gene            complement(8996..>9276)
                     /locus_tag="VIW27_13815"
     CDS             complement(8996..>9276)
                     /locus_tag="VIW27_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740800.1"
                     /translation="WGDAFTAYVEDRGYDLHTPEDYARLVADAGFRQVADADITARFV
                     DILRAELVRMETLGIEAEVRDKLAAGWRQKLDRALGGDHRWGLFTALK"
BASE COUNT         1677 a         3062 c         2947 g         1590 t
ORIGIN      
        1 gtccagccct cgccggccgg cattttcagc acatcgatgg gcacctcgat atagcacggc
       61 cgcgggcgct ggctggccag gcgggcaaag gcttcggcca cccggtcctt gaggtccttc
      121 ggcgtatagg cggtggcggc actctgggcg acgctggcgg cggcgcccga ctggtcgatc
      181 atctcgtgca ggcggccgcg cccctgggcg gcgtccttga cgtcgaggca tgaggaaatc
      241 accagcatcg gcaccgagtc ggaataggcc tgcgccatcg cggtgaggat attggtcatg
      301 cccggaccgg agatgatgaa gcaggcggcg ggcatgcccg aggcacgcgc gtatccgtcg
      361 gccatgaaac ccgcgccctg ctcgtggcgg ggcgtgatgt ggcgcagcga ggttttttcg
      421 aggccgcggt aaaactcgac cgtgtgcaca ccgggaatgc caaacacggt ggtgataccg
      481 caatcctcga gcagctcgac cagccattcg ccgcaggttc gggcttcgga gttaagggtt
      541 ttcatcgcat gacagccgct tcgggatgat ggctaatatt aacagggttc gaatcccaga
      601 cgccggcctc gcggcgcctg atatcgatcg gtgcacaagt cgcgcgccag gcaaagaaaa
      661 ttctcgacca gccggtcgcc catgtcgcgg ggatggtagc ggcgcatcag tgcgggcgcc
      721 ttgaagcgcc agatcgtgcc gagcgtttcc tcgctggcct cgggatggaa ctgggtggta
      781 tagcaatgac cgccgtaatc cagcgctgcg accggcactt tactgctgct gccgagcagc
      841 gtactgccgg cgggaatccc gacgacatgc tcgctgttgg cgtagtgaaa acaggccccg
      901 tcgccaatgc cggccatcag cggcgatgcc cggcccgcgg cggtcagttc gagcgggaaa
      961 gtgcccgcga aaggtccacc ctcgacgcgg aggacatcgg cgccctccat gtgggcaatc
     1021 agctggtgcg agccgcagat gccgagtacc ggcacccctg cgcgacgcat ctcgggcagc
     1081 caggcgcgca tccggcgttg ccacccggtg tcgtcatgca cggaattgta gctgccggct
     1141 aataccaggc cgtcgacctc gcccggaccg ggcagggtct cgccgaggca gacgttgcag
     1201 gtcgcgattc gaattccggg cactcggaaa tagcgtttca cgaaccacag cccctcctgg
     1261 tccctgtcct cgaaatggtc gtagaccgag gcatcgtagc tgccgggaac tcccatcatt
     1321 tcgacaaaca ggatgcgcgc cgccgaactc agcatttgag gctatgcatt tccggatcgt
     1381 acaacgggcc gacctgcagt gcgcagggcc ggcgctcgcc ggcgacttcc agttcgtagg
     1441 ttccggcggc aaggaacccg gcatcgacac cctcggcatg gcgcacgtaa ccgaaaccga
     1501 tattggtctc ggcggtatac ccccagccgg cgctcgacaa ccagccgacg cgttcgccgt
     1561 cacgataaat cgtttcgcgg ccgagcagaa ccacatcgcg atcggcaagc gtaaaacagg
     1621 cgagccgccg gtgcagcggc ccggctcgtt gcgcaacgat cgcatcgcgg ccgagaaaat
     1681 ccttgccgct ggcgagatcc accgcccagc cgagaccggc ctcgagcggc gtgtaatcgg
     1741 gtccgatgtc ggcaccccag gcgcgatagc ccttttccag gcgcagcgat tcgatcgcgc
     1801 ggtaaccgcc gttgacgata ccgtgcccgg cgccggccgc catcagggcc tcgtagacac
     1861 tcagcgccga ttcgaccggc acgtgcagtt cccagccgag ttcgccgacg taactgacgc
     1921 gcagcgcgcg taccagggcg cccgcgatcg ccagctcgcg catgctcgcg aacggcagcc
     1981 attcgttcgc aagatcgtcg cgcaccagcg cctgcaggat ctcgcgcgac ttcggtccca
     2041 ttagcgacag taccgcccag gccgtggtca cgtcgtcgac gcgcacgtcg agcccctgcg
     2101 gcaggttgcg ccggatccag tcgaggtcgt gggtcgcgaa gccggtgccg gtgacgaggt
     2161 agtactcgtc ctcgccgacc cgggtcacgg tcaggtcgca ctcgataccg ccgcggcgat
     2221 tgagcagctg cgtatagacc acggcgccgt cggcgacggc aacgtcgttg gcgcaaagcc
     2281 acgacagcgc gggctccgcg ccggcgccgc gaatacggta cttggcgaac gaagtctggt
     2341 cgaagacggc cgcccgctcg cgcgccgcgc ggtgctcgcg gcccacggcc tcgaaccagt
     2401 tctgccgtcc gaagccatac tcgtcgcgcg ccgatgccgg gtcatcggcg aaccagttgg
     2461 gacgttccca gccgagtttt tcgccaaagc aggcgccctg cgccttgagc gtttcgtaca
     2521 gcggcgaacg gcggctcggc cggcaggtgc ggttttcctc gaacggccag gccatcgtgt
     2581 agtgcttcga gtaagcttcc agcgtgcgtc tgcgcaccca ctcgacatcg cggtgcggct
     2641 gaccgaagcg gcgaatatcg accggccaca ggtcgaatgg tggctcgccg ttaaccaccc
     2701 actcggcgag cgccatgccg gcgccgccgc ccgctgcgat gccgaaggca ttgaagcccg
     2761 cgccgacgaa aagattcctg cgctcggggg cctcgccgag gatgaaatta ccgtccggcg
     2821 taaagctctc cgggccattg aacatctgcc gaatgccggc gtcggcgagc gccggcacgc
     2881 gcgcgatcgc ctgctccatc aacggttcga aatgcgccca gtcgttgtcc agcagggtga
     2941 aattgaaccc ctcggggatg ccgtcccgcg cccaggggat cggattcggt tcgtaaccgc
     3001 ccatgacgag gccgccgacg tcttccttga aataggtcag gcggtcggga tcgcgcagcg
     3061 tcggcagatc gggcgcgacg ccggcaatgg gctcggtaac gaggtactgg tgctgcaccg
     3121 agaccagcgg aatttcgacg cccgcgagcc ggccgaggcg gtgcgaccac tggccggcgc
     3181 aaatgaccgc gcgctcgcag cgaatcttcc cggcgtcggt ctccagcgcg cgcagcgtgt
     3241 cgccgtcgaa ctcgaagccg gtgacctcga cgccctcgcg gatcagtacc ccgcccttgc
     3301 gcgctccgct cgccagcgat tgcgtaatat cggacggatt ggcctggccg tcggtgggca
     3361 ggaaggcggc gccgaccagg tcggcaacct ccatgaccgg ccacagatcg agcgcttccc
     3421 gcggcgtcag caaatgcatc tcgaggccga aggagtgcgc ggtggttgcc tggcgcctga
     3481 cctcggtcca gcgatccgca ttgcaggcga ggcgcaggcc gccgttcatc ttccagccgg
     3541 tggcgagccc ggtctcgact tccagcgtac ggtagagttc gaccgagtaa ccgagcagct
     3601 gggtgatatt ggcattggtg cgcagttggc cgaccagccc cgcggcgtgg aaggtcgatc
     3661 ccgatgtcag gcggtgcttt tccagcaaca cgacctcgct gcagcccatc ctggcgaggt
     3721 ggtaggcggt agaacagccg atgatgccgc cgccgatgat cacgaccttc gcctcctgcg
     3781 gcaaatcggt attcgcattc atgtcactcg agcccgtatt ctcttttgcg tcgcgacgcc
     3841 caggcgctga tcatgcgatc ggagatgatc gcgatgaagg cgacacagac cccggcgacg
     3901 atgccgcgcc cggtatccgc cttggtcagc gcgatataga cttcctggcc gagttcgcgc
     3961 gtgccgacca gcgcggtgat gaccagcatg gacagcgcca tcatgatggt ctggttgacc
     4021 ccgagcatga tgtgcggcaa ggccagcggg atctgcacct tgcgcaacag ctggttgcgc
     4081 gtcatcccga cctggcgcga agcctcgatc agttcggcgg aaataccgcg aatgccgtgg
     4141 tcggtataac ggatgaccgg cgtaatcgcg taggcaatga ccgccagcat ggcggcgaaa
     4201 tcgccgaccc ggaacaacat tacgaccggc atcaggtaaa cgaagctggg cagggtctgc
     4261 aaggtgtcga taaccacgcc gaccacgcgg tgcaggcggt cgttgcgagc tgccagaata
     4321 ccgatcggga taccgataca gcaggcgatg accaccgaca ggccgcagag gtaaagagtg
     4381 accatggcct tgtcccagag tccgacgacc gcgatgaaag cggccagcgc cgcggtcagc
     4441 aggccgagcc gccggccgcc gagctgccag ccgccgaggc cgaccatcgc taccaccgcg
     4501 atccacggca gcgacagcag gaagcgtttg aacggcacca tcaggttgag tagcaggaag
     4561 ttcttgaagc tttccagcgc gtcgaagtaa ttgacgttga tatagcgcac cagctcaccc
     4621 cagaagggcc cggtggtgat ctgccagttt tcgggatagg cgttgagcgc cggcatatag
     4681 atgccgagca gcccggtcag gccgaccgcg gcgacgaaca gcagggtgtg cggatgcctc
     4741 tgccagaggc tttcgccggg ccggccatgc atcgccggcg ggcggttggc ataggcctgg
     4801 ctcaggcgat cgagcgcgat cgcgagcagc gtgatcgcca cgccggcctc gacgccctcg
     4861 ccgattcgaa gccttctcag cgcggtcagc acgtcgtaac cgagaccgcc ggcgccgatc
     4921 atcgacgcga tgatgaccat attgagggac agcatgacta cctggttgac gccgaccatc
     4981 agcgagcgct tggccgatgg cagcaaaacc ttccatagca gctggcgccg cgaacaaccg
     5041 gccatggtgc cggcctcgac gacctcggcg ccgatgtgat cgagcgccag cagggtgacc
     5101 cgcaccatcg gcggcatcgc gtagatgatc gtcgcgatca tcgccgaaac cgggctgaaa
     5161 ccgaacagca gcagcaccgg caccaggtag gcgaacaccg gcacggtctg catcaggtcg
     5221 aggattggga tcagcaggag acggaaccgc ggcgaacggt ggccgaggat gccaagcagc
     5281 atgccgccga cgacgccgat cggcacggcg atgatgattg aagacagcgt aaccatcgcg
     5341 ctgtcccatt gcccaaagac caccaggtag gcgaagcagc cgccggcgag caacgccagt
     5401 ttccagtcgc gcacgtaatg cccgagcagc atcacgccgg cgatcagcgc tagccatgaa
     5461 atacgcggga ataccggtac cgcgtcgctg ccgaccccgc tgaggaaacc ggtcgataac
     5521 aggctcctgg ccagccgata gggctgctcc accagccatg cgatccagcg agtcaagtcc
     5581 ttgaaagtga acagcccgaa atcgaagctg ttgatcaacc atttcatgaa cgcgctgatc
     5641 cagctcgcca gcggcagctg ccaggcgcgc gggtaatcga aagcccaggg tagcgcgtct
     5701 ttcgccagca ttagcccggc ggtggcggcg atcgcgaaca gccaggccag cgtccagcgg
     5761 ccggtcaggg gcagcgccgg tttcaacagg ctggccgccg cgtggctcat tcgttcgcct
     5821 gtccccgcga cagcaggtcg acgacgacct cgcgcggcag cgagccgacg gtgttgccct
     5881 cgtcgtcccg caccgcgaac acggcgccgc ggtcggcgtt caaaattgcg tcgagctggg
     5941 gctcgatcgg caggttcgcc tgtaccgtgc cggcgaaact gtcactctcg acggcacgca
     6001 tcagcgcgcc cgcggtcagc accttggcgc gcggaatgcc ctgggtgaat tcctcgacgt
     6061 aatcggtggc gggattcagg accagttctt cgggcgtgcc aatctgcatg atcgcgccgt
     6121 ccttcatgat cgcgatgcgg tcggcgagcc gtatcgcttc ctcaaagtcg tgagtaatga
     6181 agacgatggt cttgtgcagc agtttctgca ggcgcatgaa ctcgtcctgc atctcccggc
     6241 ggatcagcgg atcgagcgcc gagaagggct cgtccaggaa ccagatgtcg ggctcggcgg
     6301 cgagcgagcg cgcgatgccg acgcgttgct gctgtccgcc ggacagttcg cgcggatagt
     6361 aatgctcgcg tccctcgagg ccgaccaggc gtattacctc gatcgcgcgc gcctcacgct
     6421 ccgcgcggcc gataccctgg acctccagcg gcagcgccac gttgccgagc acggtgcggt
     6481 tcggcagcag cgcaaagtgc tgaaatacca tgcccatctt gtgacgacgc aagtcgatca
     6541 actgtgcctc gccgactttc atcaagtcct gtgattcgaa gtagatttca ccgtggctgg
     6601 gctcgatcag gcgcgtcatg cagcgcagca gggtcgactt gcctgatccg gaaagaccca
     6661 tgataacgaa ggtctccccc tcggagacgt cgaagctggc gtcgcgtacc gcgccgatgt
     6721 agccgccttg ctcgagcaca gctgtgtcgg gtctgccgcc cgcggcgaac agcgcgggca
     6781 cgccctcgcc gaacaccttc catacatgcc ggcagcggat cttgacggcg gtttcgtctg
     6841 gttgtactgg cgcgtgcggc atggtggacc ctgcccggcg ggaccgggaa tggcaggttt
     6901 cagaaaacga gggcgggccg ggacctgacc cggtacccgc cctcggcgat cgacgcgatt
     6961 actggatcca ggctttccag gtggattcgt tgtcgcgcat ccacttggcg acggtctcgt
     7021 cgaccgactt gccctcgagg tcgacctcga cgatcatctg gctcatctgc gcattgttga
     7081 tcttgaaatt gcgcaccgcc ttgtacgcgc ccggccactt cttttcaccg gctttccagc
     7141 caactttctt gatccagccg aacggcttgc cgcagtcgta ggccatgttc ttgttgatac
     7201 cccagctcgg atcctcgtaa caggcgtcgg tgtactcggg gaattcgatc cactcaccat
     7261 cgtacttggc caccgcccag tgcggcgcat agacccacag caggatcggc gccttgcgct
     7321 ggatcgccga ttcgagttcc gagaacaggg ccgcgtcggt gccggcgtgc acgacttcgt
     7381 agtcgagccc gagcgcctcg acacgctcgt cgtcaaagcc ggcccaggtt accgggccgc
     7441 cgagataacg ccccttgggc gcggtctcgg ggatcgaaaa ggcttcggca caagcgttca
     7501 gtgctttcca gtcgggaagc ccggggcatt tttccttcat atacatcggg taccaccact
     7561 cttccttggc gtccatcccg gtttcaccga gatcgaccgt attgccggtt ttcagcgact
     7621 cgtccatcgc ctgcttgccg gtggtttccc acatttccat cgcgatgtgc aggtcgcccg
     7681 actcgaggcc ggcgaactgc gcgatgtagt cggcctgcac gtattcgacg ttgtagccgg
     7741 ccttcttcag gatctcgccc atcagggtcg tggtcaggta ctggccggtc cagtcgtgca
     7801 gggtcagctt gatcggatcc ttcgattcga ccgcgcccgc gggcgccgcc agggccgcgc
     7861 aggcggcaag caggatcgat ctgagtacta cggaactgct cttcttgttc atctttatct
     7921 gtcctccctc cggattggtc gcttaccgga acggtctccg gcaggaacgg gtctttttcg
     7981 caaatttacc ccggcatgtc aacgcaatcg cccgcaaagc ggattgaacc gttcaggaac
     8041 ccgcggcaac cgccttcacg tgccgctcga acccggccgc cgacatcagc gttttgcagc
     8101 gctcgaaacg gccgatcgaa taggcccaga aatcctccgc cggattgccg tcgctgtgct
     8161 ggatcaggcc ccacagggtc cacagcaggt cgcacatggc cttgtagatg atcatacggc
     8221 cgaactcgtc ggcgccgggc tcaccgccgc accaggcgcg gatcagttcc tcgtcctgcg
     8281 cgggcgtgaa gcctgcttcc acagacaggt cgccaagatc ccacagggga tcgttcatgc
     8341 ccgagtactc ccagtcgacg atccacatgc gctcgcccga gtcgaggaaa ttttcgcaca
     8401 gcggatcgca gtggcagggc gcgagcttcg ccgggtggcg atcgagcgcc tcgcgcaccg
     8461 gctgcgccgc gtcgacgacc tcggcataac cttccggcaa ctcggccttg cgctcgtcga
     8521 gaatcttcag gtagccgtca atcatcgaga acagctcgaa gcgaaactcg aacggctcgg
     8581 accagtcgtg cattcggcga aacgcccgtc ccgcgcgccc cggcgcaccc tcgcgatccg
     8641 cgaaggccgc gggtgacatc gtgacgatgc cgtcgacatg ccgcgtcagc atgacgccgc
     8701 tttgcgcgtc cgcgtaaacg acttcggcgg agacgcccgc cttcgcggcg acccgcgcgt
     8761 tgtgcatctc caccgcgcgg tcgatatact cctcggtacc cttacccggg atacgcaaca
     8821 ccaatcgctg cgcaccggta tcgatgcggt aaacgaggtt ggtcaggccg ccgagccgtt
     8881 gcgtcgtgta cgcgtcggcc gacaggtttt cgaaacccgg tatgctggtc aggctgcggt
     8941 gcacctgctg cagatcgtcg ttcatacccc gtgtcctccc gattcttcga ttttgttact
     9001 tgagcgcggt gaaaagcccc cagcggtgat caccgccgag cgcgcggtcc agtttctgcc
     9061 gccagcccgc cgccagttta tcgcgtacct cggcttcgat gccgagcgtt tccatgcgca
     9121 ccagctcggc gcgcagaata tcgacgaaac gagcggtgat gtccgcatcg gcaacctgcc
     9181 ggaatccggc gtccgcgacc agtcgcgcgt aatcttcggg ggtgtgcagg tcgtacccgc
     9241 ggtcttcgac ataggcagtg aaagcgtcgc cccagg
//
LOCUS       DATCZV010000615         4649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_581366, whole genome shotgun sequence.
ACCESSION   DATCZV010000615 DATCZV010000000
VERSION     DATCZV010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581366"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..748)
                     /locus_tag="VIW27_13820"
     CDS             complement(173..748)
                     /locus_tag="VIW27_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HEY5740801.1"
                     /translation="MKFKIVLAALMLAPGLATAQEFDQQQLQELLGNNNIRWARHMGF
                     NPQIVRAVETQNAVELTLDEIKRRDQEWRQADPASSMLIQEVTQNEVARYFRRRVEDN
                     PSIFEVFLTDNQGANVAAYPPTSDYWQGDEEKFSNAWNGGQGQLFIGPPEFDESTNKT
                     QIQISTPVISGYQTVGVLVMGVAVDALISDE"
     gene            complement(817..1326)
                     /locus_tag="VIW27_13825"
     CDS             complement(817..1326)
                     /locus_tag="VIW27_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5740802.1"
                     /translation="MLPNEALKLDIAFDEVPQPEDVKAVESDAITSHGVLVGNIGLLL
                     PRREVSELVNNLAICRLPNTSAWFGGVTSLRGNMLPVFDLHVLLDIAHHGIERRTIVV
                     GQGEHAVAFWVDRMPRLVALSSDDLMSEAPSLPQLIADQCDEYYLRDGQVWVDWNVDG
                     FFSSLGSML"
     gene            complement(1370..1732)
                     /locus_tag="VIW27_13830"
     CDS             complement(1370..1732)
                     /locus_tag="VIW27_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5740803.1"
                     /translation="MSAKILVVDDTAAHLQQLKEIVSEAGYEVITATSGREAVEKARQ
                     EKPSMVFLDIVMDDLDGFGACREITAHQETRDIPVVFVSTKNGRADRLWAEKQGAKAL
                     LNKPVDSAEIISNLKLYA"
     gene            complement(1827..2612)
                     /locus_tag="VIW27_13835"
     CDS             complement(1827..2612)
                     /locus_tag="VIW27_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5740804.1"
                     /translation="MRETIKVHEELQLSYVGLTELEVRVLKSIFTLAPQLNENVTLTS
                     PEQVEQADIILVNADQPESLNLWKNLKQRNNLCSSLMLTDDQETADGDFVLQRPIRVQ
                     KLISAIENIVEQTSIKFPSSSGEPELRVLVVDDSFPVRKYMEHKLGELIDVPIQLHLV
                     SSGEEAIAKVASVACDIIFLDVMMDGVDGYKVCKTIKSQQNPYIVMLTSKKSPFDKVR
                     GTMSGCDAYITKPPADERLVEEIRKCLDRLGRNVSRLAVADAR"
     gene            3117..4424
                     /locus_tag="VIW27_13840"
     CDS             3117..4424
                     /locus_tag="VIW27_13840"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5740805.1"
                     /translation="MAVKNSKYARPVYLVDGSRTPFLKARGRPGPFKHADLGIHAARS
                     VLLRMPFAPDAVDEVIFGATMPGPDEANLARVVALRLGCGDRVPAYTVQRNCASGMQA
                     LDNAAMSIACGRSELVLAGGIEAMSHAPLLFHPRTVAWLADWWGAKSVVDRLKVAGRF
                     RPRMLAPIIALLKGLTDPVVGMNMGQTAEQIAWRFGITREAMDQFALDSHLKLDNGQK
                     SGALTEVVPLFDARGNTYELDDGVRPDSTLEKLARLRPAFDKPYGRVTPGNSAQITDG
                     AAALVLASESAVERHGLPVMARLVSTEWAALTPAEMGLGPAHAIAPLLKSQRLGIDDI
                     DYWEINEAFAAQVLAVIAALKDADYCREQLGLRSAVGEIPADRLNIHGGGISIGHPVG
                     ASGARIVLHLARILEQQDARTGVASLCIGGGQGGAMLLDRSVA"
     gene            4421..>4649
                     /locus_tag="VIW27_13845"
     CDS             4421..>4649
                     /locus_tag="VIW27_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid oxidation protein subunit alpha"
                     /protein_id="HEY5740806.1"
                     /translation="MNDTITDWKSERDDQDILWLTLDKQGTDTNVLSVSVLEQLSSLL
                     DEADERLPRAVVFRSGKPGGFIAGADVNEFLD"
BASE COUNT          854 a         1360 c         1407 g         1028 t
ORIGIN      
        1 atgtcggaca tgccgagcag cgccagcgaa ccgttggtca ggaagatcag taccagggtg
       61 gtgatcagga tcattaggcg tacgcctatt cgaagattgc taaacatgtt cgttaacctt
      121 gctcagttga tttgtgcctt ggttgtgcgc ttgcggcgtc gacttcggcg tattattcgt
      181 cgctgataag cgcgtccaca gccacgccca tgaccagcac gccgacggtc tgatacccgg
      241 aaattaccgg ggtactgatt tgaatctgtg tcttgttggt actttcgtcg aactcgggcg
      301 ggccgatgaa caattgtccc tgaccgccgt tccaggcgtt gctgaatttc tcttcgtcgc
      361 cctgccagta gtcactggtc ggcggatagg cggcgacatt ggcgccctga ttgtcggtca
      421 ggaacacctc gaaaatactc gggttatcct cgacgcggcg acgaaagtaa cgggcgactt
      481 cgttctgggt cacctcctgt atcaacatgc tgctggcggg gtccgcctgt ctccattcct
      541 ggtcgcgccg cttgatctcg tcgagcgtca attcaaccgc attctgggtt tcgaccgcgc
      601 gcacgatctg cgggttgaaa cccatgtgcc tggcccagcg aatattgttg ttaccgagca
      661 gctcctgcaa ttgctgctgg tcgaattcct gtgccgtcgc cagccccgga gcgagcatca
      721 gagccgctaa cactatcttg aatttcattt tcaggtttac tccgaatcga tttatcgctg
      781 acacgctccg ctgcttcgct ggtgtcgtta acatggttac agcatgctgc ccaggctgct
      841 aaaaaatccg tcgacgttcc agtcgaccca gacctgcccg tccctgaggt aatattcgtc
      901 gcactgatcg gcgatcagtt gcggtaacga cggcgcttcg ctcatcaggt cgtcgctcga
      961 tagcgcgacc aggcgcggca ttcgatcgac ccagaatgcc accgcgtgtt cgccctggcc
     1021 gacgacgatg gtgcgtcgct cgatgccgtg gtgcgcaatg tctaacagca cgtgcagatc
     1081 aaaaaccggg agcatgttgc cgcgcaggct ggtgacgccg ccgaaccagg cggacgtgtt
     1141 cggcaagcgg caaatagcca ggttattgac cagttcactg acttcccggc gtggtaacag
     1201 cagaccgata ttgccgacca gcacgccgtg cgaggtgatt gcatcggact cgacagcctt
     1261 gacgtcctct ggctgcggca cctcgtcgaa agcgatatcg agtttcaggg cttcgttggg
     1321 tagcatcggt caatctggct cgttggtgtt gtcgcagcag ctctgccggt caggcgtaga
     1381 gtttcaggtt gctgatgatt tccgcgctgt cgacaggctt gttcagcaaa gctttggcgc
     1441 cctgcttctc cgcccataag cggtcggcgc ggccgttttt ggtgctgacg aagaccactg
     1501 gaatatcccg ggtctcctga tgcgcggtga tttcccggca ggcgccgaaa ccgtcgagat
     1561 cgtccatgac gatatcgagg aaaaccatgc tcggcttttc ctggcgagcc ttttcgacgg
     1621 cttcgcgccc cgaagtcgcg gtgatcacct cgtaacccgc ctcggaaacg atctccttga
     1681 gttgctgcag atgcgcggcg gtgtcgtcta ctacgagtat ttttgctgac actgtgtttt
     1741 ctcctgaccc tgcgctaccg ttacgtctct cggtgtcggg accgtgaaag ctgtcgatcg
     1801 aactggtggc tgaaagaacg gcatgttcag cgcgcgtcgg cgaccgccag cctgctaaca
     1861 ttcctgccga ggcgatcgag gcatttgcgg atttcctcga ccaggcgctc gtcggccggc
     1921 ggcttggtga tgtaggcgtc gcaacccgac atggtgccgc gcaccttgtc gaacggcgat
     1981 ttcttgctgg tcaacatgac gatgtacggg ttttgctgcg acttgatggt cttgcaaacc
     2041 ttgtaaccgt cgacaccgtc catcatcacg tcgaggaaaa tgatatcgca ggcaacgctg
     2101 gcaaccttgg cgatcgcttc ttcgccgctg gagacgagat gcagttgaat cggcacgtcg
     2161 atcagttcgc cgagcttgtg ctccatgtac ttgcgtaccg gaaaactgtc gtcgaccacc
     2221 agtacgcgga gttcgggttc gccgctggag ctcggaaact tgatcgaggt ctgttcgacg
     2281 atattctcga ttgccgaaat cagtttctgt acgcggatcg gccgctgcag tacgaagtca
     2341 ccatcggccg tttcctggtc atcggtcagc atcagcgaac tgcaaaggtt gttgcgctgc
     2401 ttgaggtttt tccacaggtt cagcgattcg ggctggtcgg cgttgaccag gatgatatcg
     2461 gcctgctcga cctgctcggg tgatgtcagt gtgacgtttt cgttgagttg aggggcaagc
     2521 gtaaaaatgc ttttcagcac cctcacctcc agctcggtca agcctacgta actcagttgt
     2581 aattcttcat gcaccttgat cgtctcccgc atatccaggt ttccataccc tttgtcgaat
     2641 ccccttcgaa gtgcccgcag taccggaccc ttgccagcgc ggatcgtcaa gattgttgct
     2701 tgctaccccg tcgctcgcac tcccaggtat ggtttgttac accgctgtca tctatgccgc
     2761 acgtccccgg aaccagtttc gttccccgcc gcgtaaccgg cttcgtactg ctcggttcag
     2821 tctgtttctg tatagcccat aatgggggga agtaaaggcg atccggcggt cgccgggggt
     2881 ctgaaaagat atttattcga aacttgacgt gtctcacaat tccagcggcc ggaaatggca
     2941 tttcgtgatt tttccccggt tgaatttggc ttgcggattc agaatcgaaa acaccctgaa
     3001 cgagcctgtg cgagcggcaa gttgctattg gcgcgagcgg attttgggtt accattgggt
     3061 tgcgcgtcga agccgcgcct cagccaacag cgcgaatatt gctcagggag ccattgatgg
     3121 cggtaaaaaa cagtaaatat gcgaggcccg tttacctggt cgacggcagc cgtaccccgt
     3181 ttcttaaggc gcgcggcagg ccggggccct tcaagcatgc cgatctcggt attcatgcgg
     3241 cgcgttcggt gctgctgcgc atgccgttcg ctcccgatgc cgtcgacgaa gtcatttttg
     3301 gtgccacgat gccaggcccc gacgaggcca acctggcgcg cgtggtcgcg ctcaggctcg
     3361 gttgcggtga ccgggtaccg gcttataccg tgcagcgcaa ttgcgcgtcc ggaatgcagg
     3421 cactggacaa cgcggcgatg tcgatcgcct gcggccgctc cgagctggtg cttgccggcg
     3481 ggatcgaagc catgagccac gcgccgttgc tgttccatcc acggaccgtc gcctggcttg
     3541 ccgactggtg gggggcaaag agcgtcgtcg atcggctcaa ggtcgccgga cgctttcgcc
     3601 cgcgaatgct ggcaccgata atcgccctgc tcaagggact caccgacccg gtggtcggta
     3661 tgaacatggg gcagacggcg gaacaaattg cctggcggtt cggtatcacc cgcgaagcca
     3721 tggaccagtt cgcgctcgac agccacctga agctcgacaa cgggcaaaaa agcggcgcgc
     3781 tgaccgaggt ggtgccgcta ttcgatgcgc gtggcaatac ctatgagctg gacgatggcg
     3841 tgcgcccgga ctcgacgctg gagaaactgg cgcgcctgcg gccggcgttc gataagccct
     3901 acggccgggt cacccccggc aacagcgcgc agatcaccga cggcgcggcg gcgctggtgc
     3961 tggctagcga gagcgcggtc gaacggcacg gattaccggt gatggcccga ctggtcagca
     4021 ccgagtgggc ggcgttgacc cctgcggaaa tggggctcgg accggcgcac gcgatcgcgc
     4081 cgctgttgaa atcacaacgg ctcggcatcg acgatatcga ttactgggag atcaacgagg
     4141 cattcgccgc ccaggtgctg gcggttatcg ccgccctcaa ggacgccgat tactgccgtg
     4201 agcagctcgg attgcgctcg gcagtcggcg aaataccggc tgacaggctc aatattcacg
     4261 gtggcggcat cagtatcggt cacccggtcg gcgccagcgg cgcgcgcatc gtgctgcatc
     4321 tggcgcggat cctcgaacaa caggatgccc ggaccggggt cgcgtctctg tgcattggcg
     4381 gcggtcaggg cggcgcgatg ctgctcgaca ggagcgtggc atgaacgaca ccattaccga
     4441 ctggaaaagc gagcgcgacg accaggacat cctgtggctg acgctggaca aacagggtac
     4501 cgataccaat gtgttgtcgg tatccgtgct cgagcagttg agcagcctgc tcgacgaggc
     4561 cgacgaacgc ttgccgcgcg cggtggtgtt tcgctccggc aagccgggcg gctttatcgc
     4621 cggcgccgac gtcaatgaat ttctcgatg
//
LOCUS       DATCZV010000616         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_208904, whole genome shotgun sequence.
ACCESSION   DATCZV010000616 DATCZV010000000
VERSION     DATCZV010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208904"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="VIW27_13850"
     CDS             <1..1850
                     /locus_tag="VIW27_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY5740807.1"
                     /translation="GIFSDPLTLGALGGVVVLLLALLLYRRKKNETESDSTITASNDE
                     DLFVDDDQEELTPIHLDDAEVAEDSIIGSGASEEPVPSSEQSMAPSEAVSAAEPQADD
                     FAATAIISPVDVPQPEAPAAVAPEEQDDVLNEVDVYLAYGLYDNAEELLTNNLAARPD
                     RVDYRSKLLDTYFATKNTAAFVKEAETLKSMGTAAGRFWDRVQVMGYELAPDNPLFSD
                     AKDSGMSAADLEISKPQEADFDLGTAGSEDTSFSTTDFNLGEDEAGGQVDDVAVAATQ
                     VIQQVEDLTDLGDDLPDLGDAADFAPTEVVRQVGGTVELSDDDDTNLNRQSADDDFSL
                     DLSDDAGEELEFSMDEGAGGEDAAAGGDLDLSMDFDIDDEVSGGDSAEFSLDDASSME
                     DPSEFNLGDDLAASIGDDTEAAAALGLDLDEQTGGDDFIEETAVISADFDATIFDTSE
                     DESASEDLGLSIDETGVLDLDDFDSALDDTDDDDDISIVDFGGEDFEEPTALVDSIDD
                     LDDSLSIDVDIVGDEDEVKTGTFSPGDFDEPTAVASAVDDLGDLGDLDDMDDLMLPDD
                     VDEVSTKLDLARAFIDMGDTEGARGSLEEVLAEGNEAQKQEAQALLDQI"
     gene            2074..2859
                     /gene="truA"
                     /locus_tag="VIW27_13855"
     CDS             2074..2859
                     /gene="truA"
                     /locus_tag="VIW27_13855"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY5740808.1"
                     /translation="MKYALGVEYSGSAYCGWQRQPHCSSIQQHLEEALAFVADHPVQL
                     VCAGRTDAGVHAIEQVAHFESVAERSARSWVLGTNCRLPRDIRVLWVTPVSDDFHARF
                     GARARAYRYLIINSSVPSAIFHDRASWERRPLDHEVMHECAQVLLGEHDFSSFRAVGC
                     QARSTRREVQEISVCRRDDLVYLDIRANAFLYHMVRNIAGSLMAVGKGEQDREWFGEV
                     FAARDREQADVTAAAAGLYFLRAWYEPQHELPTRGKKPVLFEP"
BASE COUNT          597 a          896 c          936 g          535 t
ORIGIN      
        1 gtggaatttt cagcgatccg ctaacactcg gcgcgctcgg cggcgttgtc gtgctgctgc
       61 tggctctgct gctataccgt agaaaaaaaa acgaaactga aagcgactcg acaataacgg
      121 caagcaatga cgaagactta ttcgtcgacg acgaccagga agaactaacc cccatccatc
      181 tcgacgatgc cgaggtggcc gaagacagca taatcggctc cggcgccagc gaggagccgg
      241 tgccgagcag cgagcaatcg atggctccta gcgaggcggt ttccgcagcc gagccgcaag
      301 ccgatgattt cgcggcgacc gcgatcattt cccccgtcga cgttcctcaa cccgaggcgc
      361 cggcggcggt cgcccccgag gagcaggatg acgtcctcaa cgaggtcgac gtctacctgg
      421 catatggtct ttacgacaat gccgaggaat tgctgaccaa caacctggcc gcgcgcccgg
      481 atcgcgtgga ttaccgctcc aagctgctgg atacctattt cgcgaccaag aataccgcgg
      541 ccttcgtcaa ggaagccgaa acgctgaagt cgatgggcac tgccgcgggc cgcttctggg
      601 accgcgtaca agtcatgggt tacgagctgg cgcccgacaa cccgctgttc tccgatgcca
      661 aggacagcgg catgagcgcc gccgacctgg aaatctccaa gccccaggaa gccgatttcg
      721 atctcggcac agcaggtagc gaggatacca gtttctcgac caccgatttc aatttgggtg
      781 aagacgaagc cggcggccag gtggatgacg tcgcagtcgc ggcaacccag gtgattcagc
      841 aggtcgagga cctgacggat ctcggcgacg acctgcccga cctcggagac gccgccgatt
      901 ttgccccgac agaggtcgtg cggcaggtcg gtggaacggt ggaactttcc gacgacgacg
      961 acaccaacct gaatcgacag tcggccgacg acgatttctc tctcgatctg tccgacgatg
     1021 ctggcgagga actcgaattc tcgatggacg agggtgccgg cggtgaagac gccgcggccg
     1081 gcggggatct cgacctgtcg atggatttcg acatcgacga cgaggtcagc ggcggagact
     1141 ctgcggaatt ttctctcgac gacgcgtcgt cgatggagga tccgtcggag ttcaatctcg
     1201 gcgacgacct ggcggcgtcg atcggtgacg acacagaggc cgccgccgca ctcgggctgg
     1261 acctggacga gcagaccggc ggcgatgact tcatcgaaga gacggcggtg atctccgcag
     1321 acttcgatgc taccatattc gacacctcgg aagacgaatc cgccagcgaa gatctcggtc
     1381 tcagcatcga cgaaacgggc gtgctggacc tcgatgactt cgattcagcg ctcgatgata
     1441 ccgatgacga cgatgacatt tccatcgtcg acttcggcgg cgaggacttc gaggaaccga
     1501 ctgcgctggt ggattcgatc gacgacctcg acgattcgct gtcgatagat gtggatatcg
     1561 tgggcgacga ggacgaagtc aagaccggca ccttttctcc gggggatttc gacgagccga
     1621 cggcggttgc cagtgcggtc gacgacctcg gtgacctcgg cgatctcgac gatatggacg
     1681 atctgatgct gccggacgac gtcgacgagg tgtcgaccaa gctcgacctg gcgcgtgcat
     1741 tcatcgacat gggggatacc gagggggccc gcggcagcct cgaggaagtg ctcgccgagg
     1801 gtaacgaggc gcaaaaacag gaggcgcaag cgctgctcga tcaaatctag gctggccccg
     1861 ttcgaacgcc tctttgctgc gcgggcgccg gccgcgcgtc agccgtaagc gcggtctttt
     1921 actcgaaaat gcccgctatc gaagcttgca atttgctaaa ctccgctgct cgtcgaacag
     1981 agcgtcgatc gtcttgacct tgccaccctt cacagcccgt gatattcgct gcccgatgtc
     2041 ggccgccgcg ctcgccgagg gggtggggtg gcggtgaaat acgctctcgg ggtcgagtac
     2101 tcggggagtg cctattgcgg ctggcagcgg caaccgcact gcagctcgat tcagcagcac
     2161 ctcgaggaag cgcttgcttt tgtcgccgat cacccggtac agctggtttg cgccgggcgc
     2221 accgacgcag gcgtgcacgc catcgaacag gttgcccatt tcgaaagcgt tgccgagcgc
     2281 agcgcgagat cctgggtgct gggcaccaat tgccggctgc cgcgcgatat tcgagtgctg
     2341 tgggtgacgc cggttagcga tgattttcac gcccgcttcg gtgcccgcgc ccgtgcttat
     2401 cgctacctga tcatcaactc atcggtgccg agcgccatat tccacgatcg cgccagctgg
     2461 gaacgccggc cgctggatca cgaggtgatg cacgaatgcg cgcaagtgct gctcggcgag
     2521 cacgatttca gcagctttcg cgcggtgggt tgccaggcca ggtcgacccg cagggaggtg
     2581 caggaaatat ccgtctgcag gcgcgacgac ctggtatatc tcgatatcag ggccaatgct
     2641 ttcctctatc acatggtgcg caatatcgcc ggcagcctga tggcggtcgg caagggtgaa
     2701 caggaccgcg aatggttcgg cgaggttttc gcggcccgcg accgcgagca ggccgatgtc
     2761 accgccgcag ccgccggact gtattttctg cgtgcctggt acgaaccgca acacgagctg
     2821 cccacgcgcg gcaaaaaacc cgtattattc gaaccatgaa acagcgtacc agagtcaaga
     2881 tttgtggaat tacccgcctc gaggacgcgc gccatgccgc ggccatgggt gcggatgcga
     2941 taggcctgat gatgcatccg ccca
//
LOCUS       DATCZV010000617         4004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_208948, whole genome shotgun sequence.
ACCESSION   DATCZV010000617 DATCZV010000000
VERSION     DATCZV010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208948"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VIW27_13860"
     CDS             <1..356
                     /locus_tag="VIW27_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5740809.1"
                     /translation="IGIASEQDWYTEYLAPILSIRIVDGLEQAIDHINRYGSRHTDAI
                     VSEHYGNARRFLRSVDSSSVMVNASTGFADGFEYGLGAEIGISTDKLHARGPVGLEGL
                     TSQKYVVFGDGNIRA"
     gene            358..975
                     /gene="nadD"
                     /locus_tag="VIW27_13865"
     CDS             358..975
                     /gene="nadD"
                     /locus_tag="VIW27_13865"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEY5740810.1"
                     /translation="MIGIFGGTFDPIHNGHLHIARAVLALLNLQQLQFVPCARPVHRD
                     PPRATAAQRCHMIGLALAGEPRMRLNRLEIERGGFSYTVDSLREIRRTEDASLVLLLG
                     ADAFNGLASWESPAEILELANLAVCLRPAVEVSPDLYAAARVDSVEALGASRAGAILL
                     LEVDAPDCSSSALRAGLAAGRQSSCLPAAVADYIRKQHLYRRPGD"
     gene            968..1327
                     /gene="rsfS"
                     /locus_tag="VIW27_13870"
     CDS             968..1327
                     /gene="rsfS"
                     /locus_tag="VIW27_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY5740811.1"
                     /translation="MTDSDPANLQSAAIRKLAVQALEDLKAENIVQLDVRRQASFTDC
                     MIFASGNSTRHVGAIAEAVIEAAKAAGQPPLGVEGEDVGEWVLVDLGDAIVHVMLPDV
                     RRYYELEKLWGEELAPE"
     gene            1400..1870
                     /gene="rlmH"
                     /locus_tag="VIW27_13875"
     CDS             1400..1870
                     /gene="rlmH"
                     /locus_tag="VIW27_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY5740812.1"
                     /translation="MQIQIINVAHKLPAWAEQACEDYLRRMPRELSPKLLTIPLAARS
                     ARQPAAAVQRREAEAILGKLAPGSFNLALDERGSCWSSNDWAQNLQRWMLEQPRVNLI
                     IGGPDGLADDCIRACQQRVALGRMTLPHALVKVVLIEQLYRAWTIIQGHPYHRE"
     gene            2072..2647
                     /locus_tag="VIW27_13880"
     CDS             2072..2647
                     /locus_tag="VIW27_13880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY5740813.1"
                     /translation="MIYLASASPRRAQLLRQIGVEFEVCSSDIDETWAAGEDAADYVR
                     RLAVDKARSAAREIVARDANYRVLAADTTIALDDDIIGKPANPHECRRILERMSARQH
                     RVLTAVALATPAGIALRLNESRVRFRALSAAEIAAYCACDEPMDKAGAYAIQGKAAIF
                     VEHLDGSYSAVMGLPLLETAELLRDAGLDIM"
     gene            2659..>4004
                     /gene="rng"
                     /locus_tag="VIW27_13885"
     CDS             2659..>4004
                     /gene="rng"
                     /locus_tag="VIW27_13885"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY5740814.1"
                     /translation="MSEEILLNVTPQETRVAIVENGALQEVCIERQRSRGIVGNIYKG
                     KVNRVLPGMEAAFVDIGLEKAGFLHVSDIDVGGDLEDETDSSPRPAINELLREGQTLL
                     VQVVKDPMGTKGARLTTSISVPSRYLVYMPNSPVVGISVKIEDESERERLKNLLEACR
                     SENQIGGCIIRTAAEGVDEAELKRDIAFLSKLWGSLLEYADNAPPGSMIHEDLPLSIR
                     TLRDMVGSQTTLVKVDSRETFQKMTQFAARYIPDLPAGIEHYPGERPLFDLYNIDDEL
                     QRALARQVSLKSGGYLIIDQTEAMTTIDINTGAFVGHQNLEETIFRTNMEASQAIARQ
                     LRLRNLGGIIIIDFIDMEREEHKRQVLKSLEKHLEKDHAKTQICEVSPLGLVEMTRKR
                     TRESLEHVLCEACKTCHGRGSIKTTETVCYEIFREILREARLYEEARQLLVMANQDV"
BASE COUNT          751 a         1269 c         1315 g          669 t
ORIGIN      
        1 ggatcggcat tgccagcgag caggactggt atacggaata cctcgcgccg atcctgtcga
       61 ttcgcatcgt cgacgggctc gagcaggcga tcgatcacat caatcgttac ggctcgcggc
      121 acaccgatgc gatcgtcagc gagcattacg gcaatgcgcg ccgttttctg cgcagcgtcg
      181 attcgagctc ggtcatggtc aacgcctcga ccggcttcgc ggacggcttc gagtacggac
      241 tcggcgccga aatcggcatc agcaccgaca agctgcacgc gcgcgggccg gtcggactgg
      301 aaggcctgac gtcgcagaag tacgtggtgt tcggagacgg caatatccgc gcctgagatg
      361 atcggcattt tcggcggcac cttcgatccg atccacaacg ggcatttgca tatcgcgagg
      421 gcggtgttag cgctgctgaa cctgcaacag ttgcagttcg tgccctgtgc caggccggtg
      481 caccgcgacc cgccgcgggc gacggccgcg cagcgttgcc atatgatcgg gctggcgctc
      541 gccggcgaac cgcgcatgcg cctcaaccgg ctcgaaatcg agcgcggcgg attctcttac
      601 acggtcgaca gcctgcgcga gatacggcgt accgaggatg ccagcctggt gctgctgctc
      661 ggcgcggatg ccttcaacgg actcgccagc tgggagtcgc cggcggagat tctcgagctg
      721 gcgaacctgg cggtttgcct gcggcccgcc gtcgaggtga gccccgatct gtatgcggcg
      781 gcgcgggtcg actcggtcga agccctcggc gcgagccggg cgggcgcgat cctgctgctc
      841 gaggtcgacg cgccggattg ctcttccagc gcgctgcgcg ccggactcgc cgccggccgg
      901 caatcgtctt gcctgccggc cgcggtcgcc gactatattc gtaaacagca tctttaccgg
      961 aggccgggtg actgattccg atcccgccaa cctgcaatcc gcagcgattc gcaagctggc
     1021 cgtacaagcc ctcgaggatc tcaaggcgga aaacatcgtg cagctcgacg tgcgccgcca
     1081 ggcgagcttc accgactgca tgattttcgc cagcggcaac tcgactcgcc acgtcggtgc
     1141 gattgccgag gcagtgatcg aggcggcaaa ggcggccggc cagccgccgc tcggcgtcga
     1201 gggcgaggac gtcggcgaat gggtactggt ggacctcggc gacgcgatcg tgcacgtcat
     1261 gctgccggac gtgcgccgtt actacgagct ggaaaaactg tggggcgagg aactggcgcc
     1321 cgaatgactg tcgacgggcc cgcggacacg cgggccgcat tcgacgtcgc tccgtgcgct
     1381 tgcagacagc ctatcgaaga tgcagattca gatcatcaac gtcgcccaca aactgcccgc
     1441 ctgggccgag caggcctgcg aggattacct gcggcgaatg ccgcgcgagc tgagcccgaa
     1501 attgttgacg atcccgctgg cggcgcgcag cgcccggcaa ccggccgccg ccgtgcagcg
     1561 ccgcgaagcc gaggcgattc tcggcaagct ggcgcccggc agcttcaacc tggcgctcga
     1621 cgagcgcggc agctgctggt cgagcaacga ctgggcacag aatctgcagc gctggatgct
     1681 cgagcagccg cgcgtcaacc tgatcatcgg cgggcccgac ggcctcgccg acgattgtat
     1741 tcgggcttgc cagcagcgcg tggcactcgg caggatgacc ctgcctcatg cgctggtgaa
     1801 agtcgtgctg atcgagcagc tctaccgtgc ctggactatc atccagggac atccctacca
     1861 ccgcgagtag cccgcggatt gcatcagccg aactaacgcc gcaaaacatg tcatcacatc
     1921 agtcgcagca actcaattcc ccggtccgct cgaggtgcca gccgatggtg atcgttggcg
     1981 cgcgtcccgt tgcggcgccg gttgccgcat cgcccggtcg cggcgcgctg ccggccgcgg
     2041 cccgggcgcg gtcaggggct tcgaggccgc gatgatctac ctggcttccg cttcgccgcg
     2101 ccgcgcgcag ctgctgcgcc agatcggcgt cgagttcgag gtctgttcga gcgacatcga
     2161 cgagacctgg gcggctggcg aggacgccgc cgattacgtc cgccggctgg ccgtcgacaa
     2221 ggcgcgctcg gcggcgcggg aaatcgttgc ccgcgacgcg aattaccggg tgttggccgc
     2281 cgatactact attgcgctcg atgatgatat tattggcaag cccgcgaacc cgcacgagtg
     2341 ccggcggatt ctcgagcgga tgtcggcccg gcagcaccgt gtactgaccg ccgttgcgct
     2401 ggcgacgccc gccggcatcg cgctgcgcct caacgagagc cgggtcaggt ttcgcgccct
     2461 gtcggcggcg gagatcgccg cttattgcgc ctgtgacgaa cccatggaca aggcgggcgc
     2521 ctatgcgatc cagggcaagg cggcgatatt cgtcgaacat ctcgacggca gttattccgc
     2581 cgtcatggga ctgccgctgc tggaaaccgc ggaactgctg cgcgacgccg gcctggacat
     2641 aatgtgaagt attagacgat gagcgaagaa atcctgttga acgtgacacc ccaggaaacc
     2701 cgggtggcta tcgtcgagaa cggcgccctg caggaagtct gcatcgagcg ccagcgcagc
     2761 cgcggaatcg tcggcaatat ctacaagggc aaggtcaacc gggtattgcc gggtatggag
     2821 gccgctttcg tcgatatcgg tctcgaaaag gccggcttcc tgcacgtctc ggatatcgat
     2881 gtcggcggcg atctcgaaga cgaaaccgac agcagtccgc ggccggcgat caacgaattg
     2941 ctgcgcgagg gacagaccct gctggtgcag gtggtcaagg atccgatggg caccaagggc
     3001 gcgcgcctga ccaccagtat ctcggtgccg tcgcgttacc tggtgtacat gccgaactcg
     3061 ccggtggtcg gcatttcggt caagatcgaa gacgaaagcg aacgcgagcg gctgaaaaac
     3121 ctgctcgaag cctgccgttc cgaaaaccag atcggcggct gcatcattcg caccgcggcc
     3181 gagggcgtcg acgaggccga actgaaacgc gacatcgcgt tcctgtccaa gctgtggggg
     3241 tcgctgctgg agtatgccga caacgcgccg cccggcagca tgatccacga agacctgccg
     3301 ttgtcgatcc gtacgctgcg cgacatggtc ggcagccaga cgacgctggt caaggtcgac
     3361 tcgcgcgaaa ccttccagaa gatgacgcag ttcgcggccc gttatattcc cgacctgccc
     3421 gccgggatcg agcattaccc gggcgagcgg ccgctgttcg acctgtacaa tatcgacgac
     3481 gagctgcaac gcgcgctggc gcggcaggtg agcctgaaat cgggcggtta cctgattatc
     3541 gaccagaccg aggcgatgac cactatcgat atcaacaccg gtgcgttcgt cggtcaccag
     3601 aatctcgagg aaaccatctt caggaccaat atggaggctt cgcaggcgat cgcacgtcag
     3661 ctgcggctgc gcaatctggg cggcatcatc atcatcgact tcatcgacat ggagcgcgaa
     3721 gagcacaagc gccaggtact gaagtcgctg gaaaagcatc tcgagaagga tcatgccaag
     3781 acgcagatct gcgaggtctc gccgctcggc ctggtggaaa tgacgcgtaa gcgcacgcgg
     3841 gaaagcctcg agcacgtgct ctgcgaagcc tgcaaaacct gtcacggcag gggatcgata
     3901 aagaccacgg aaaccgtgtg ctacgagata tttcgcgaga tcctgcgcga ggcgcggctt
     3961 tacgaagaag cccggcagct gctggtgatg gccaaccagg atgt
//
LOCUS       DATCZV010000618         6063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_473416, whole genome shotgun sequence.
ACCESSION   DATCZV010000618 DATCZV010000000
VERSION     DATCZV010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473416"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VIW27_13890"
     CDS             complement(<1..2226)
                     /locus_tag="VIW27_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5740815.1"
                     /translation="MNLARASVRRPVFASMLTLIVVVLGAMAVGRLQIDLLPSIELPT
                     LTVRTQYEGADPVVMERLVTQIVEEIVATVPGVEEMTSQSYEGNSRVRVSFGWGADID
                     TVAVDVQATLADEASELPDDIVGPRVSKFDIDSFPVVLLGISSPLDPVELTQLVEDQI
                     RYRFARIPGVAQVDPWGGFTRQVRVELDPARINALGLPLNDMLDKIRDANLDLPAGKL
                     ESGRFEVTLRAPAEFQSLDEIRATVIAERDGAQVSLGQVAVVKDTYEKLSRIIRINGE
                     RGVRIAIRKQAEANTVEVSRRVLDEIEAINRAFPQLHVTPVINQGNFIERSIKNVARS
                     VLYGSALAVLVLLFFLRNLRSTAVIFLAIPISMLATFALLFFGGFTINLMTLGGLALG
                     AGLMLDASVVVLENVFRRYQERGEKPSRAAIAGTREVASAVTAGTVTTLTIFLPLIFL
                     EGVTGVLFRELAYVIMFSLACALLVALALVPMLASKLLAASAGRSGRGLEAGAAAAFV
                     RLENGYRDLLEITLNHRFTVIGLAAALFAASLLLLPYIGTEFMPPSDEGEVRVSGEME
                     VGTRLDLLDRQTRLMEALVYDEVPEAHASVVSITPSGTWGRSKAQGEIRLSLSPAAER
                     SRSNEEIARELRTLLDGKIPGMKIRTRAPQGQFLLQRIIDNPEGITIEVRGFDLDALG
                     LLSQQVAAAIGDVPGITDVEISRESGVPQQQIKVLRDKIADLGLDVRDVTEVIETAVA
                     GS"
     gene            complement(2223..3422)
                     /locus_tag="VIW27_13895"
     CDS             complement(2223..3422)
                     /locus_tag="VIW27_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5740816.1"
                     /translation="MHKYRGSTRLKSGGKTVLVVTVVLLTGLLGRGVYDKLESGRVEV
                     REQRPRAAAPVEVAVVEIGPIEQLRSFTGTLEARAEFLAAPKIAGRIEVLQVDLADRV
                     ERGQVIAVLDDAEYVQQVARARADLAVEKASHAEALDQLTIAERELQRLEPIHQRGEL
                     SASQLDVTRSQLVAKRALTEVTRARIARAEAELESARIRLGYTQVKADWQGGNRTRVV
                     AQRFVDAGETVAANAPLLRIVELNPITAVFFVTERDYARLEVGQQAILGTDAWPGIEF
                     GGRVERISPVFSESTRQARVELQVDNPDRRLKPGMFIRATVTLQRIDDAVIVPELALT
                     RRDGRDGVFMVDDDGRTARWHIVEPGIRQAGRVQLSGAELSGQVVVLGQQLLDDGAEL
                     QVVEVQR"
     gene            3558..4418
                     /locus_tag="VIW27_13900"
     CDS             3558..4418
                     /locus_tag="VIW27_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008555747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEY5740817.1"
                     /translation="MTDNNETAAPADAAARRAAKALVCYAVDQLPPNNLPLYRAARAG
                     MQKTAELTVPPRDAACFEVAAGSFFRIVSVDGPQVGDLNLWNTADLSERFFSGKTRQL
                     HATHVSTGDRLWSSLPGFRPLATITHDTLDWYGWDDDGGGVHDVIGTRCDPYTNYLLK
                     GVDYHHCCHSNLTRALAAARKLPLNEAELHVHDVLNVFMCTGFTRDTHQYFMKASPVR
                     PGDFIEFFAEIDLLGALSTCPGGDCGSSHSDDATPCYPLRVEIFEPAAGSLDGWTPPV
                     PSGYSRTHGL"
     gene            complement(4444..>6063)
                     /locus_tag="VIW27_13905"
     CDS             complement(4444..>6063)
                     /locus_tag="VIW27_13905"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY5740818.1"
                     /translation="GTRVCYRAPTPVDYCNRLQHLAWNGELAFDLAELEGDALPLSAS
                     GDLTLADFESRSNVLSRALANFQRFAVEEIVIDTDAAIAVGSVTLESLKLFERVSKDD
                     SPFIQQVDKLTIRDTRLANGQLLTIAEIEVLGNATELVKSESDGFELDAWLAALGRPP
                     QADAAPADASDSTAAGIEASAPTNSEEAAATTPAEPFAFSLDRFVFRTEKPLQYRDLS
                     LGKPFTLGISTTELVLEKLDSRASEQPSTITYQAKLDGDSLFKLEGTATPLAEKISFD
                     IDGEIRALDLRRLSPFTAASIGHTIKSGQLDADIDLQAEQAIMNGKIDLVLYRFDLQP
                     LSEADAAEMEKEFGFPLGVSLSLLKNRQGNIELAIPLSGDLENPDFGTGKIVSRELSK
                     AITSAVLTFYTPFGMVTAADALFSLATALKFEPVVFEPGESEIGEAEIGDLDKIAALM
                     QERPGIQLRVCPYTNSADRADLLPTTATTAAEDLKLVSSQLALLSELGEARFAAVEKY
                     LVEKGADAARVIPCAPSHEEGEGLGGVEISI"
BASE COUNT         1015 a         1982 c         1974 g         1092 t
ORIGIN      
        1 cgagcccgcg actgcggttt cgatcacctc ggtgacgtcg cgcacgtcca gcccgaggtc
       61 ggcaatcttg tcgcgcaaca ccttgatctg ttgttgcggt acgccgctct cgcggctgat
      121 ctcgacgtcg gtgatgccgg gcacgtcgcc gatcgccgcg gctacctgct gcgacagcag
      181 gccgagcgca tcgaggtcga aaccgcgtac ctcgatggtg attccctcgg gattgtcgat
      241 gatacgctgc agcaggaatt gtccctgcgg cgcgcgggta cggattttca tgcccgggat
      301 cttgccgtcc agcagggtgc gaagctccct ggcaatttcc tcgttgctgc ggctgcgctc
      361 ggccgccggc gacagcgaca ggcgaatttc accctgcgcc ttcgagcgcc cccaggtgcc
      421 cgacggcgtg atcgacacca ccgaggcatg cgcctcgggt acttcgtcgt acaccagcgc
      481 ttccatcagc cgcgtctggc ggtcgagcag gtctaggcgg gtgccgactt ccatctcgcc
      541 gctgacgcgc acctcgccct cgtcgctggg tggcatgaac tcggtgccga tgtacggcaa
      601 cagcaacagg ctggcggcaa acagtgccgc ggccagtccg atgaccgtga agcgatggtt
      661 cagggtgatc tcgagcaggt cgcggtaacc gttctcgagg cgcacgaaag ccgccgcggc
      721 gcccgcttcg aggccgcggc cggaccgccc ggcgctcgcc gccagcagtt tcgaggccag
      781 catcggtacc agtgccagcg ccaccagcag cgcgcaggcg agcgaaaaca tgatcacgta
      841 ggcgagttcg cgaaacagca cgccggtcac gccttcgaga aaaatcaatg gcaggaagat
      901 ggtcagcgtg gtcaccgtgc cggcggtcac cgccgaggct acttcgcggg tgccggcgat
      961 cgccgcgcgc gacggctttt cgccgcgctc ctggtaacgg cggaacacgt tttccagtac
     1021 caccaccgat gcgtcgagca tcaatccggc gccaagcgcc agcccgccga gggtcatcag
     1081 gttgatcgtg aagccaccga agaacagtag cgcgaaggta gccagcatgg aaatggggat
     1141 cgccaggaat atcaccgccg tgctgcgcag gttgcgcagg aagaacagta gcaccagtac
     1201 cgccagcgca ctgccgtaga gcaccgagcg cgcgacgttc tttatcgagc gctcgatgaa
     1261 attgccctgg ttgatgaccg gagtgacatg cagttgcgga aatgcgcgat tgatcgcctc
     1321 gatttcgtcg agcacccgcc gcgatacctc gaccgtgttg gcctcggcct gcttgcggat
     1381 cgcgatgcgc acgccgcgct cgccgttgat gcgaatgatg cgcgacagtt tttcgtaggt
     1441 atccttgacc accgccacct ggccgagact gacctgcgcg ccgtcgcgct cggcgatcac
     1501 cgtggcgcgt atttcgtcga ggctctggaa ctccgccggc gcgcgcagcg tgacctcgaa
     1561 gcggccgctc tccagcttgc cggccggcag atcgaggttg gcgtcgcgaa tcttgtcgag
     1621 catgtcgttg agcggcaggc cgagcgcgtt gatgcgcgcc gggtcgagct cgacgcgcac
     1681 ctggcgcgtg aaaccgcccc aggggtcgac ctgggcgacg ccggggatac gcgcgaagcg
     1741 atagcggatc tggtcctcga ccagctgcgt cagctccacc gggtcgaggg ggctggaaat
     1801 gccgagcagt accaccggga agctgtcgat gtcgaacttg gatacccgcg ggccgacgat
     1861 gtcgtcgggc agttcgctgg cctcgtcggc cagcgtcgcc tgcacgtcga ccgcgacggt
     1921 atcgatatcc gcgccccagc cgaagctgac gcgcacgcgg ctgttgccct cgtaggactg
     1981 cgaagtcatt tcctcgaccc ccggcacggt ggcgacgatt tcttcgacga tctgcgtcac
     2041 cagccgctcc atgaccaccg ggtcggcgcc ctcgtactgg gtgcgcacgg tcagcgtcgg
     2101 cagctcgatg ctcggcagca ggtcgatctg caggcgcccc actgccatcg cgccgagcac
     2161 gacgacgatc agcgtcagca tcgacgcgaa taccgggcgg cggaccgagg cgcgcgccag
     2221 gttcatcgct gcacctcgac gacctgcagc tcggcgccgt cgtcgagcag ttgctggccg
     2281 agcaccacca cctgccccga caattcggcg ccgctgagct gtacccgccc agcctggcga
     2341 atgccgggct cgacgatatg ccagcgcgcc gtgcggccgt cgtcatcgac catgaatacg
     2401 ccgtcgcggc catcgcgccg cgtcaatgcc agttccggaa cgatcaccgc atcgtcgatg
     2461 cgctgcagcg tgaccgtggc gcggatgaac atgcccggtt tcaggcggcg gtcgggattg
     2521 tcgacctgca gctcgacgcg cgcctggcgc gtcgattcgc tgaacaccgg agaaatgcgc
     2581 tcgacgcggc cgccgaattc gatgccgggc caggcgtcgg tgccgaggat cgcctgctgg
     2641 ccgacctcga gccgggcgta gtcacgctcg gtgacgaaaa ataccgcggt aatcgggttc
     2701 agttcgacga tgcgcagcaa cggcgcgttg gcagcgacgg tttcaccggc gtcgacgaag
     2761 cgttgcgcga ccacgcgcgt gcggttgccg ccctgccagt cggccttgac ctgggtataa
     2821 ccgagacgga tgcgcgccga ctcgagttcg gcctcggcgc gcgcaatgcg tgcccgcgtg
     2881 acctcggtca gggcccgctt ggcaaccagt tgcgaacggg tgacatcgag ttgtgaggcc
     2941 gacagttcgc cgcgttggtg aatcggctcg agacgttgca gttcgcgttc ggcgatcgtg
     3001 agctggtcga gcgcttcggc gtggctggct ttttcgaccg ccaggtcggc acgcgcgcgg
     3061 gccacctgct gcacgtactc ggcatcgtca agtaccgcga tcacctggcc gcgctcgacg
     3121 cggtcagcga gatcgacctg cagcacctcg atacggccgg cgatcttggg cgcggcgaga
     3181 aattccgcgc gcgcctcgag agtgccggtg aagctgcgca actgctctat cgggccgatt
     3241 tcgacgaccg caacctcgac cggcgccgcg gcgcgcgggc gctgttcgcg aacctccacc
     3301 cggccgcttt cgagcttgtc gtaaacgccc cggccgagca ggccggtcag cagcacgacg
     3361 gttaccacga ggacggtttt gccgccggac ttcaacctgg tcgaccctct atatttgtgc
     3421 aagtcgcggt cagtttaagc cgattcgcaa ccgagggaaa ctgcccgcgc aactccgcgg
     3481 ccatagcaca aagccagacc ccgcggcggc gaatgcgcta ccatggccgt cgagttcatc
     3541 gacccgcagc tgccatcatg accgacaaca acgaaaccgc cgcgccggcc gatgccgctg
     3601 cgcgccgcgc cgccaaagcg ctggtctgct atgccgtcga ccagttgccg ccaaacaatc
     3661 tgccattgta ccgcgccgcg cgcgcgggta tgcaaaagac cgccgagttg accgtgccgc
     3721 cgcgcgacgc cgcgtgtttc gaggttgccg ccggatcttt ctttcgcatc gtcagcgtcg
     3781 acgggccgca ggtcggcgat ctcaacctgt ggaataccgc ggatctgtcg gagcgcttct
     3841 tcagcggcaa gacacgccag ctgcacgcca cccacgtcag caccggcgac cgcctgtgga
     3901 gcagcctgcc cggattcagg ccgctggcga cgatcaccca cgatacgctc gactggtacg
     3961 gctgggacga cgacggcggc ggcgtgcacg atgtcatcgg cacacgctgc gatccgtata
     4021 ccaattacct gctgaagggt gtcgattacc atcactgttg tcattcgaat ctgacgcgcg
     4081 cactggcggc ggcgcgcaag ctgccgctca acgaggccga gttgcacgtg cacgacgtgt
     4141 taaatgtttt catgtgcacc ggtttcaccc gcgacacgca ccagtatttc atgaaggcga
     4201 gcccggtgcg ccccggcgac tttatcgagt tctttgccga aatagacctg ctgggcgcac
     4261 tgtcgacctg tccgggcggc gattgcggca gcagtcattc cgacgacgcg acgccctgtt
     4321 atccgctcag ggtcgaaatc ttcgagccgg cggcgggcag tctcgacggc tggacgccgc
     4381 cggtgccgag cggctactcg agaactcacg ggctgtagcc tgctgcgcga ggcgggacgg
     4441 tcctcagata ctgatttcga cgccgcccag gccttcgcct tcctcgtggc tcggcgcgca
     4501 ggggatgacg cgcgccgcat cggcgccttt ttcgaccagg tatttctcga ccgcggcgaa
     4561 acgggcctcg cccagttcgc tgagcagcgc cagctggctg ctgaccagct tcaggtcttc
     4621 cgccgccgtg gtcgcggtag tcggcagcaa gtccgcgcga tcggcgctgt tggtgtaggg
     4681 acacaccctg agctggatgc cgggtcgctc ctgcatcagg gccgctatct tgtcgagatc
     4741 gccgatttct gcttcgccga tttcgctttc acctggctcg aagacaacgg gctcgaactt
     4801 gagtgccgtc gccaggctga acagcgcatc ggccgcggtg accatgccaa acggcgtgta
     4861 gaaagtcagt accgcgctgg taatcgcctt tgacagttcg cggctgacga tcttgccggt
     4921 gccgaaatcg gggttttcga ggtcgccgga gagcggtatt gcgagctcaa tattgccctg
     4981 gcggttcttc agcagcgaca ggctgacgcc gagcgggaag ccgaattcct tctccatttc
     5041 cgcggcatcg gcctcgctca ggggctgcag atcgaagcgg tacagcacca ggtcgatctt
     5101 accgttcatg attgcctgct cggcctgcag gtcgatgtcg gcatcgagct ggccgctctt
     5161 gatcgtgtgg ccgatgctgg cagccgtgaa cggtgaaagc cggcgcaagt ccaatgcacg
     5221 aatctcaccg tcgatgtcga aggagatttt ctccgccagc ggcgtcgccg tgccttccag
     5281 cttgaacagg ctgtcgccgt ccagttttgc ctgataggta atcgtgctgg gctgttcgga
     5341 cgcgcggctg tcgagctttt ccagcaccag ctcggtggtg ctgatcccca gcgtgaaggg
     5401 cttaccgagg ctcaggtcgc ggtactgcag cggcttttcg gtgcggaata cgaagcggtc
     5461 cagcgaaaag gcgaagggtt cagcgggtgt cgtggccgcg gcttcttcgc tattcgtggg
     5521 ggcactcgct tcgatgcctg ccgcggtcga gtcgctggcg tctgccggag cggcgtccgc
     5581 ttgcggcggc cggccgagag cagcgagcca ggcatccagc tcgaagccgt cggattcgga
     5641 tttgaccagc tcggtggcat tgcccagcac ctcgatttcg gcaatggtca gcagctggcc
     5701 gttcgccagg cgggtatcgc gaatcgtcag cttgtcgacc tgctggatga acggactgtc
     5761 gtccttgctg acgcgttcga acagcttcaa cgactcgagg gtcacggaac caacggcgat
     5821 tgcggcgtcg gtgtcgataa cgatttcctc gacggcgaag cgttggaaat tcgccaatgc
     5881 ccggctgagg acgttgctgc ggctttcaaa gtccgccagg gtcaaatcgc cgctggcgct
     5941 gagcgggagc gcgtcgcctt cgagttcggc aagatcgaat gccagctcac cgttccacgc
     6001 cagatgttgc agccggttac aatagtcgac cggtgtcggg gcgcggtagc aaacccgggt
     6061 tcc
//
LOCUS       DATCZV010000619         2958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_420576, whole genome shotgun sequence.
ACCESSION   DATCZV010000619 DATCZV010000000
VERSION     DATCZV010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420576"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..494)
                     /locus_tag="VIW27_13910"
     CDS             complement(156..494)
                     /locus_tag="VIW27_13910"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HEY5740819.1"
                     /translation="MKLRMMALVILLGIAGRGEAGFYTGSEVLSNCQHSTESGKSFCA
                     QYLGGIVDATEIWAGAGLMPRLICMPAETSSDRLREIFVAYANQNPEELHEVASATVI
                     AALIGAFPCE"
     gene            924..1523
                     /gene="lexA"
                     /locus_tag="VIW27_13915"
     CDS             924..1523
                     /gene="lexA"
                     /locus_tag="VIW27_13915"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY5740820.1"
                     /translation="MLTSQEQKTLQFIRNYLAQYGYAPKFKEIGRAIGVNSHGTIHRY
                     VQALEDKGYIDRAKGHSRGMHLKELPLVSPPTIPLAGRIAAGLPIEAIEDQQELNLAE
                     MFMGPELFALRVTGDSMIDAGILDEDYVIIRKQPVANDGDIVVAMIDKSEATLKRFKR
                     RGDDQVALIPENPDMEPMIYPAERVTIHGVLVGQLRNYR"
     gene            complement(1694..2482)
                     /locus_tag="VIW27_13920"
     CDS             complement(1694..2482)
                     /locus_tag="VIW27_13920"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5740821.1"
                     /translation="MQPMLNIAIRAAHRAGDFIVRKINKLPDLKVEVKAQNDYVSEVD
                     RDAEARIIDELRKAYPDHGIIAEESEAIDADADYLWIIDPLDGTTNFLHGFPHYAVSI
                     ACQHRGQLQHGVIYDPFKQELFAASRGVGATLNNRRIRVASKKSLAGTLLATGFPFRN
                     PEQVDEFMKLLRLLYTSAGDIRRAGSAALDLAYVAAGRLDGYWESGLKAWDLAAGALI
                     VREAGGLVTDYAGDGGFLDNGQVVAANPRVTAEMLRIIQQGLKS"
     gene            2580..>2958
                     /locus_tag="VIW27_13925"
     CDS             2580..>2958
                     /locus_tag="VIW27_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY5740822.1"
                     /translation="MKLENISIVLVGTTHPGNIGATARAMHNMCFSRLVLVDPQCPVG
                     EIAYARASGAHAVLDGRRTCDDLQSAIADCHYVIAASARRRSLNWPELEPSRMAQTLL
                     DLDQDRRAAIVLGREHSGLTNDEL"
BASE COUNT          597 a          885 c          899 g          577 t
ORIGIN      
        1 cttgccgata tctgcatggc ggagagttcg attgcggtgc tccgattcat cctgaacggc
       61 ctactcgttg ccggacctgg ccaccaatcg gttgctgcgg tgattgttgc ccagggactc
      121 gaatgcaggt gcgcgccggc aattgacaac tggctctatt cgcacggaaa agcgccgatc
      181 aaagcggcga tcaccgtggc gctggcgacc tcgtgcagtt cttcagggtt ctggttggcg
      241 taggctacaa agatttcccg taacctgtcc gaggaggtct cggcgggcat gcagatcagc
      301 ctcggcatga ggcccgcgcc agcccagatc tcggtggcat cgacgattcc tccgaggtac
      361 tgcgcgcaaa acgatttacc gctctcggtg ctgtgctgac aattcgacag aacttcactg
      421 ccggtgtaga aaccggcttc accgcgcccg gcaatcccca gcaggataac cagcgccatc
      481 attcgcaatt tcaagatcta ctccagctga ttgatgctcg cgaaaccgtc tcatatcgcg
      541 cgcggctaaa caagttgtcg tacgagtgaa ttggcccggc aaataaatac ggttttcaga
      601 accctcggtg ctacgggcac tattcatggc tacgagaggc agctggcaac cgatcgtcgg
      661 gcggtgttgc ggcatcgata atggtcctgc cgacagagcc ccgaatccac cgcaaggctg
      721 cgatcggcag cggtcggccg ttggccctgg ctggttgtca gcagccctgt gtatcgaaaa
      781 cgggcgcagt gcgcattaag aatcggctac gatcggcgac tggccacggc agtcccgaaa
      841 tgagccgcat cgctcgcccc gggttgcatg ttctcgtttt tttctctaca ctcgctgaga
      901 ataaaacgag aaggtttcgc ccgatgttga cttcccagga acaaaagaca ctgcagttca
      961 tccgcaacta cctggcacag tacgggtacg cgccgaaatt caaggaaatc ggccgcgcga
     1021 tcggcgtcaa ttcgcatggc acgatccacc gctacgtgca ggcgctcgag gacaagggtt
     1081 atatcgaccg cgccaagggt cactcgcgtg gtatgcatct caaggaactg ccgctggtca
     1141 gcccgccgac gattccgctg gccggccgaa ttgccgccgg cttgccgatc gaggcgatcg
     1201 aggatcagca ggaactgaat ctcgcggaaa tgttcatggg ccccgaactg ttcgcgctgc
     1261 gggtgaccgg cgattcgatg atcgacgccg gcatcctcga cgaggactac gtgatcattc
     1321 gcaagcagcc ggtggccaat gacggcgaca tcgtcgtcgc gatgatcgac aagtccgaag
     1381 ccacgttgaa gcgtttcaag cgccgcggcg acgaccaggt ggcgctgatt ccggaaaatc
     1441 ccgacatgga gccgatgatt tatcccgccg agcgggtgac gatacacggc gtgctggtcg
     1501 ggcagttgcg caactaccga tagcaacgca tcgagttttc cccgacggcg cgatttgcga
     1561 ccgcgaacag tcgcagggga gggtgaagct tctcgatcaa ggcccgcgaa cagccctgca
     1621 gcggcggttt gctcgctgac cggtagtcgg ctggacgata tttaagcgct cgccacagcc
     1681 acccggccgc cattcaggat ttcagtccct gttgaatgat acgcagcatt tccgcggtga
     1741 cccgcgggtt ggccgcgacc acttgaccgt tatcgagaaa accgccgtcg ccggcgtagt
     1801 cggtgaccag tccgccggcc tcgcgcacga tcagcgcacc cgcggccagg tcccatgcct
     1861 tgagacccga ttcccagtaa ccatcgaggc ggccggccgc gacataagcc agatcgagtg
     1921 ccgccgaacc ggcgcgacgt atgtcgccgg cggaggtata aagcaggcgc agcagtttca
     1981 tgaattcgtc gacctgctcg ggattgcgga acgggaaacc ggtcgccagc agcgtgccgg
     2041 cgagagattt cttgctggcg acccgaattc gccggttgtt gagcgtggca ccgactccac
     2101 ggctggcggc gaacaattcc tgcttgaacg gatcgtagat cacgccgtgc tgcaattgcc
     2161 cacggtgctg gcaggcgatg gacaccgcgt agtgcggaaa gccgtgcaga aaattggtgg
     2221 tgccgtcgag cggatcgatt atccagaggt agtcggcatc ggcgtcgata gcctccgatt
     2281 cctcggcgat gattccgtga tccgggtaag ccttgcgcag ttcgtcgatg atacgcgcct
     2341 cggcatcgcg gtcgacttca ctgacatagt cgttttgcgc ttttacttcg accttcaagt
     2401 cgggtagctt gttgattttg cgaacgataa aatccccggc gcgatgggcc gctcgaatcg
     2461 ctatgtttag catgggttgc atcgggattt taccgggctc gaagaaggcg cgcagtctag
     2521 caaagggtcc acggattaac cagtatttca ccacgcgccc gctgccagga gaaactcgat
     2581 tgaagctcga aaatatcagc atcgtattgg tcggcacgac ccatcccggc aatatcggag
     2641 ccactgcgcg ggcgatgcac aatatgtgct tcagccgact ggtgctggtg gatccgcaat
     2701 gcccggtggg ggaaatcgcc tatgcgcgcg cctccggtgc ccacgcggtg ctcgacggcc
     2761 gccgaacctg cgacgatctg cagtcggcga ttgccgattg ccattacgtg attgccgcca
     2821 gcgcgcggcg gcgaagcctg aactggcctg aactcgagcc gtcgcgaatg gcgcagactc
     2881 tgctcgatct cgaccaggat cgccgggccg cgatcgtact gggccgcgaa cactcggggc
     2941 tgaccaacga cgaattgc
//
LOCUS       DATCZV010000620         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_367587, whole genome shotgun sequence.
ACCESSION   DATCZV010000620 DATCZV010000000
VERSION     DATCZV010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367587"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1303
                     /locus_tag="VIW27_13930"
     CDS             155..1303
                     /locus_tag="VIW27_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY5740823.1"
                     /translation="MTEANKPAMETRLAQACHYIDTATGGISPPLQLSTTYARGPDYE
                     YLGDYSYSRAGNPSWAALETVCAEIDGGVEALCFASGMAAVCAVFETLEAGQHVAAPR
                     VMYHGTQDWLRHLAAKRSIGLTLFDTADPAALERAVEPGKTAIVWIESPVNPTWELID
                     IAAAAEVAHRAGAVLAVDATVSTPVTTRALEHGADIVFHSATKYLNGHSDVVGGILIS
                     AADDARWQQIKWLRTYMGGVLGPFEAWLLLRGMRTLFLRFERASASALEIARHFEHHP
                     QLERVLYPGLESHPGHQIARRQMTCGFGGMLSLLVEGGAEAARRIAGNTRLFVRATSL
                     GGVESLIEHRAAVEGPHSAVPPNLLRLSIGIENCRDLIDDLERSLDTS"
     gene            1320..1583
                     /locus_tag="VIW27_13935"
     CDS             1320..1583
                     /locus_tag="VIW27_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740824.1"
                     /translation="MSVEESVEIATEAIMKSLSRHELSDAEKEEISRIVGRLLVKTVK
                     KTTRNQVETAINCCGPEKDLAHQLAEEMHRRKNLLISNLMALR"
     gene            1839..>2323
                     /locus_tag="VIW27_13940"
     CDS             1839..>2323
                     /locus_tag="VIW27_13940"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEY5740825.1"
                     /translation="MNRYSAWAVFKNGLTGQKHWDRQWRDPEPKKAYDVVIVGAGLHG
                     LATAYYLAKNHGVRNIAVLEKGWLGGGNAGRNTTIVRSNYMMPGNRQFYEHSLKLWEN
                     LSHELNYNVMFSQRYHVSLLHSPAARDAAARRYNTMRLTDSDGELWDLDTLKAEIPHL
                     N"
BASE COUNT          469 a          690 c          745 g          419 t
ORIGIN      
        1 cttgcgctcc gcgtcggtga tcgaaaacgg cttgctggcg gccatcgggc taatctctga
       61 tggttgaaaa cgttgccaag aataaatgaa gatcggtgtt aagatacatt attttttaaa
      121 cgaattttgt agatagttgt aaacgaaaaa cccgatgacc gaagccaaca agcccgccat
      181 ggagactcgc ctcgcgcaag cctgccatta catcgacacc gcgaccggcg gtatcagccc
      241 gccgctgcag ttgtcgacga cctacgcgcg cgggcccgat tacgaatacc ttggcgacta
      301 tagctacagc cgcgccggca atccgagctg ggcggcgctg gaaacggttt gcgccgagat
      361 cgacggcggg gtcgaggcgc tgtgttttgc atccggcatg gcggcggtgt gcgcggtttt
      421 cgagaccctc gaggcaggcc agcacgtggc tgcgccgcgg gtcatgtatc acggcacgca
      481 ggactggctg cggcacctcg ccgcaaagcg ctcgattggc ctgacgctgt tcgacactgc
      541 ggatccggcc gcgctggagc gggccgtgga gcccggcaag accgccatcg tgtggatcga
      601 gtcgccggtt aatccgacct gggaactgat cgatatcgcc gccgccgccg aggtagcgca
      661 ccgtgccggc gcggtgctgg cggtcgatgc caccgtatcg acaccggtaa ccacgcgcgc
      721 tctcgagcat ggcgccgata tcgtgtttca ctccgcgacc aagtacctca atggccacag
      781 cgacgtggtc ggcggcattc tgatcagcgc cgcggacgac gctcgctggc agcaaatcaa
      841 gtggctgcgg acctacatgg gcggcgtgct cgggccgttc gaggcctggt tgctgttgcg
      901 cggcatgcgc acgctgttcc tgcgcttcga acgggcctcg gccagcgcgc tcgagatcgc
      961 ccgccatttc gagcatcacc cgcaactgga acgcgtgctc tacccggggc tcgagagcca
     1021 tcccgggcac caaatcgcgc gccggcagat gacctgcggc ttcggcggca tgctgtcgct
     1081 gctggtcgag ggcggtgccg aggcggcgcg ccgcatcgcc ggcaataccc ggctgttcgt
     1141 gcgcgcgacc tcgctgggcg gtgtcgaaag ccttatcgag caccgcgcgg cggtcgaggg
     1201 gccgcacagc gcggtgccgc caaacctgct gcggctgtcg atcgggattg aaaattgccg
     1261 ggacttgatc gatgatctcg aacgaagcct ggataccagt taatagacga ggagggtata
     1321 tgtcagtaga agaatccgta gaaatcgcca ccgaggcgat catgaaatcg ttgtccaggc
     1381 acgagttgtc cgacgccgaa aaagaagaaa tcagccgtat cgtcggcagg ttgctggtca
     1441 agacagtcaa gaaaacgacc cgcaaccagg tcgagacggc gatcaattgc tgtggcccgg
     1501 agaaagacct ggcgcatcag ctcgccgagg aaatgcaccg caggaagaac ctgctgatct
     1561 ccaacctgat ggcattacgc tagccggttt gctcgccgtg gcgatatgtc gcagcggcgc
     1621 gagcgggaat ctggcttcgg cgctgccgcc aataaaactg gcgagctcgc ggggatgaca
     1681 tcaaaaccgt cgggggatgc cggcgctcga gggtgtcgca tttgtcggtt ttattggcgc
     1741 gttacgcgaa tttcggggcg ctggcttgtt gttataatcg acagcctgcg gtgggtgcgg
     1801 gcaatccgcg ccaaaacggc tggcaacggg aactcacttt gaatcgttat tcggcatggg
     1861 ccgttttcaa gaacggcttg acgggacaaa agcactggga ccgccaatgg cgcgatccgg
     1921 agccaaaaaa ggcctacgat gtggtcatcg tcggcgccgg tctgcacggc ctggcgaccg
     1981 cctattacct ggcgaagaat cacggtgttc gcaatatcgc ggtgctcgaa aagggctggc
     2041 tcggcggtgg caacgcgggg cgcaacacca ccatcgtgcg ctccaactac atgatgcccg
     2101 gcaaccggca gttctacgag cattcgctga agctgtggga aaacctgagc cacgagctca
     2161 attacaatgt catgttcagc cagcgctacc acgtgtcgct attgcacagc ccggcggcgc
     2221 gggacgccgc ggcgcggcgc tacaacacca tgcggctcac cgattccgac ggcgagctgt
     2281 gggatctcga tacactgaag gcggaaattc cgcacctaaa tta
//
LOCUS       DATCZV010000621         4452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_102752, whole genome shotgun sequence.
ACCESSION   DATCZV010000621 DATCZV010000000
VERSION     DATCZV010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102752"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1990)
                     /gene="parE"
                     /locus_tag="VIW27_13945"
     CDS             complement(104..1990)
                     /gene="parE"
                     /locus_tag="VIW27_13945"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY5740826.1"
                     /translation="MKNQYDAASIEVLTGLEPVRKRPGMYTDTTRPNHLAQEVIDNSV
                     DEAIAGHATQIGVILHADQSLSVIDDGRGMPVDIHPQQKIPGVEVILTKLHAGGKFSN
                     KNYQFSGGLHGVGVSVVNALSKRLDVRVRRDGKEYTIGFAGGDKKKKLEQTGSVGKKN
                     SGTSLRFWPDPKYFDSPTFSVSRLKHVLRAKAVLCPGLRVKFYVEKTKESAEWCYQDG
                     LSDYLLEALEGCELLPPEPFCNSSSGEHEAVDWAVVWLPQGGDAVTESYVNLIPTSQG
                     GTHVNGLRTGITDAIREFCEFRSLLPRGVKIAPDDVWDKVSFVLSVKMEEPQFSGQTK
                     ERLSSRETAAFVSGVAKDSFSLWLNQHADIGDQLAQLAISSAQSRLKSSKKVVRKKIT
                     QGPALPGKLADCSSQDVERTELFLVEGDSAGGSAKQARDRAFQAIMPLRGKILNTWEV
                     PPEEVLASQEVHDISVAIGVEPGSDNLANLRYGKICILADADSDGAHIATLLCALFLR
                     HFRPLVDKGHVYVAKPPLYRIDVAKEKFYALDEAERDGILDRIKAEKRKGKVSVTRFK
                     GLGEMNPLQLRESAIAPDTRRLAQLTIAPGDKTFKIMDMMLGKKRAADRKNWLQTKGD
                     LAEL"
     gene            2092..2955
                     /gene="trxA"
                     /locus_tag="VIW27_13950"
     CDS             2092..2955
                     /gene="trxA"
                     /locus_tag="VIW27_13950"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5740827.1"
                     /translation="MSESPYIHNVGMQNFQQLVLEKSMQKPVLVDFWADWCQPCQTIM
                     PMLAKLADEYAGKFELAKVNADDERELAGHFGIKSLPTMKLFFQGQLVDERLGAVPES
                     EVRAMLDKHIVSESEQYMQAAMTAYQQGQTEQALQLLNHALANDPENPELKVTIAQIV
                     HAQGDTDSAVRLLDSLDEEGAKLDGAISLRAQIKLAEQLASLPPLEDIEARLASDPGD
                     LEALLFKSRHLTARQDYENAMECLLEIMRRDREYQDEAGRQGLLELFDMLGGEHPSVQ
                     QYRRKLFTLLH"
     gene            complement(2980..3888)
                     /gene="xerD"
                     /locus_tag="VIW27_13955"
     CDS             complement(2980..3888)
                     /gene="xerD"
                     /locus_tag="VIW27_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY5740828.1"
                     /translation="MPAKDNRGDEYIDRFLDRLWAQKGLAELTLRAYRQDLQLFSRWL
                     AERGQDLPRADQSSIQRYLAERFEGGASASSNARLLSTLKQYYRHLVRDGERGDNPTA
                     LISAPKIHRVLPQSLAESEIDKLLEAPDLDSPYGLRDRCMLELMYGSGLRVSELVGLR
                     LDQVNTNLGLVRLVGKGSKERVVPVGEEALYWLGRYLGKARPELINSAQPGDALFPSS
                     RGSAITRQAFWQNIKKYLLKAGVKTVFSPHSLRHAFATHLLNHGADLRTVQMLLGHSS
                     LSTTQIYTHIAQARLASMHAEHHPRG"
     gene            complement(3944..4381)
                     /locus_tag="VIW27_13960"
     CDS             complement(3944..4381)
                     /locus_tag="VIW27_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740829.1"
                     /translation="MANAKKSAKPKAKKAAVKKKAAGSAVKKKVATKKKVAANKKAAA
                     PAAKKKAVKKKASRPVVKKTATAAAKKRPAAKKKAGPSVKEKALQKKVAELTKLNKKL
                     AAAGKAQDKVVKGLKSQLSAAGKAAAAAAKAAKPRKKAKAKKK"
BASE COUNT          824 a         1369 c         1297 g          962 t
ORIGIN      
        1 cggtcgagga tgacgtacat cgagtagtcg aggtaagcct tctcggtaaa cacgtgcagc
       61 ggctgctgct cgacgccctc gtaattgagt tccaggtttt cgctcatagc tcggcgagat
      121 ctcccttggt ctgcagccag ttcttgcggt cggcggcgcg cttcttgccc agcatcatgt
      181 ccatgatctt gaaggtcttg tcgccgggcg cgatcgtcag ttgtgcgagc cgccgcgtat
      241 ccggcgcgat cgcgctttcg cgcagctgca acggattcat ttcacccagc cccttgaagc
      301 gcgtcaccga gaccttgccc ttgcgctttt cggccttgat gcgatcgagg atgccgtcgc
      361 gctcggcctc gtcgagcgcg taaaactttt ccttggcgac atcgatgcga tacagcggcg
      421 gcttggcgac gtagacatgc cccttgtcga ccagcgggcg aaaatggcgc aggaacagcg
      481 cgcacagcag ggtcgcgata tgcgcgccgt cggaatcggc atcggcaaga atacagatct
      541 tgccgtagcg caggttggcg aggttgtcgg aacccggctc gacaccgatg gcgaccgaga
      601 tgtcatgcac ttcctgcgag gccagtactt cttcgggcgg cacttcccag gtattcagaa
      661 tcttgccgcg caatggcatg atcgcctgga aggcgcgatc gcgcgcctgc ttggccgagc
      721 cgccggccga atcgccctcg accaggaaca gctcggtgcg ctcgacgtcc tggctgctgc
      781 aatcggccag cttgcccggc agcgccgggc cctgggtgat cttcttgcgc acgactttct
      841 tcgagctctt gagccggctc tgcgcgctgg aaatcgccag ctgcgcgagt tgatcgccga
      901 tgtcggcgtg ctggttgagc cacaggctga aggaatcctt ggcgaccccg gaaacgaagg
      961 cggcggtttc gcgcgacgac agccgctcct tggtttgccc ggaaaactgc ggctcttcca
     1021 tttttaccga gagcacgaag ctgaccttgt cccagacatc atcaggagcg atcttgacgc
     1081 cgcgcggcag caggctgcgg aactcgcaaa actcgcggat cgcgtcggta atcccggtgc
     1141 gcaggccatt gacatgagta ccgccctgcg aggtcgggat caggttgacg tagctctcgg
     1201 tgactgcgtc accaccctgc ggcagccaga cgaccgccca gtcgacggct tcgtgctcac
     1261 ccgacgaaga gttgcaaaac ggctccggcg gcagcagttc gcagccttcc agtgcctcga
     1321 gcaggtaatc cgacagcccg tcctggtagc accattcggc agactccttg gttttttcga
     1381 cgtagaactt gacgcgcagt cccgggcaca gcaccgcctt ggcgcgcagc acatgcttga
     1441 ggcgggacac cgaaaacgtc gggctgtcga aatatttcgg atccggccag aagcgcaggc
     1501 tggtgccgga attcttcttg ccgacgctac cggtctgctc cagctttttc ttcttgtcgc
     1561 cgcccgcgaa accgatcgta tattccttac cgtcgcggcg cacgcgcacg tcgagccgct
     1621 tcgacagcgc gttgaccacc gatacgccga ccccgtgcag gccgcccgaa aactggtaat
     1681 tcttgttgga aaacttgccg ccggcatgca gcttggtgag gatgacctcg acccccggaa
     1741 ttttttgctg cgggtgaata tcgaccggca tgccgcggcc gtcgtcgatc accgacagcg
     1801 actggtcggc atgcagaatc acgccgattt gcgtcgcatg tcccgcgatc gcctcgtcga
     1861 cactgttgtc gatgacctcc tgggcgagat ggttgggtcg cgtggtgtcg gtatacattc
     1921 ccggtcgctt tctgaccggt tcgagccccg tcaggacttc gatcgaggcg gcatcgtact
     1981 gatttttcaa aagtggcggt tcctgagtat caagccatcg aacggctgat gcacttgtat
     2041 ttcagggctt gcggcctgat ataatgcgca tcattttgcg ggtcccctat tatgagcgag
     2101 tcaccctata ttcacaatgt cggcatgcag aatttccagc aactggtgct cgaaaaatcg
     2161 atgcagaaac cagtgctggt ggatttctgg gccgactggt gccaaccctg ccagaccatc
     2221 atgccgatgc tcgcgaagct cgccgacgag tacgccggca agttcgaact ggccaaggtc
     2281 aacgccgacg acgagcgcga gctggccggg catttcggca tcaagagcct gccgacgatg
     2341 aaactgtttt tccaggggca gctggtcgac gagcggctcg gcgcggttcc cgaatccgag
     2401 gttcgcgcga tgctcgacaa gcatatcgtg tcggaatccg agcagtacat gcaggcggca
     2461 atgacggctt accagcaggg acagaccgag caggcgctgc aactgctcaa ccatgcgctg
     2521 gccaatgacc cggagaaccc cgaactcaaa gtcacgatag cgcagatcgt gcatgcccag
     2581 ggcgacaccg acagtgcggt caggctgctc gacagtctcg acgaggaagg cgccaaactg
     2641 gacggcgcga tcagcctgcg cgcgcagatc aagctggccg agcaactcgc gagcctgccg
     2701 ccgctcgagg atatcgaggc gcgcctcgcc agcgaccctg gcgatctcga agccctgctg
     2761 ttcaagagcc gccacctgac cgcccgccag gattacgaaa acgcgatgga atgcctgctc
     2821 gagatcatgc gtcgcgaccg cgaataccag gacgaggccg gtcgccaggg cttgctcgaa
     2881 ttgttcgaca tgctcggcgg cgaacacccc agcgtccagc agtaccgccg aaaactcttc
     2941 accctgttgc actgacgcgc aaattcagcg gcgagtcgct caaccgcgag ggtgatgctc
     3001 ggcgtgcatc gacgccagcc gcgcctgcgc gatgtgcgta tagatctgtg tcgtcgacag
     3061 gctgctgtgc cccagcagca tttgcactgt acgcaggtcg gcaccgtggt ttaacagatg
     3121 cgtggcaaag gcatggcgta acgaatgcgg tgagaacacc gtctttacgc cggccttcaa
     3181 caggtacttc ttgatattct gccagaacgc ctggcgcgtg attgcgctac cgcggctcga
     3241 cgggaacagt gcgtcgccag gctgcgcgct gttgattaat tcggggcgcg cctttccgag
     3301 gtagcgaccc agccagtaaa gcgcttcctc gccgaccggc accacgcgct ccttgctgcc
     3361 cttgccgacc aggcgcacca gcccgagatt ggtatttacc tggtcgaggc gtaaaccgac
     3421 cagttcgctg acgcgcagtc ccgatccata catcagctcg agcatgcagc gatcgcgcaa
     3481 tccgtagggg ctgtcgaggt ccggggcttc gagcagcttg tcgatctccg actccgccag
     3541 cgattgcggc agcacgcgat gaatcttagg cgcgctgatc agcgcggtcg gattgtcgcc
     3601 gcgctcaccg tcgcgcacca ggtgccgata atactgcttt aatgtcgaca acagccgggc
     3661 gttggaactt gccgaggcac cgccttcgaa acgctcggcg agatagcgct gaatgctcga
     3721 ttgatcggca cgcggcagat cctggccgcg ctcggcaagc cagcgggaaa aaagttgcaa
     3781 gtcctgtcga taggcgcgca atgtcagttc ggcgaggcct ttttgcgccc acagccggtc
     3841 gagaaagcga tcgatatact cgtcgccgcg attatctttt gcaggcatca ataaaaaagg
     3901 cacagcgagc tgtgcctttg agaaagtttc tgtcgcgcag acactatttc tttttggctt
     3961 tcgccttctt cctgggcttg gcagctttcg cggcagcggc ggcggccttg cctgctgcac
     4021 tcaactgact cttcaaaccc ttgacaacct tgtcctgcgc ttttcctgcg gcggccagtt
     4081 tcttgttcag cttggtcagc tcggcgactt tcttttgcag cgctttttcc ttcaccgaag
     4141 ggccggcttt cttctttgct gcaggccttt tcttggctgc tgccgttgcg gtcttcttaa
     4201 caacaggccg gcttgccttt ttctttacag cttttttctt ggctgcgggc gctgcggctt
     4261 tcttatttgc cgctactttt ttctttgtcg ctactttctt ctttactgcg gaaccggcag
     4321 cttttttctt cactgcggct ttctttgctt tcggtttggc tgactttttg gcattcgcca
     4381 tcatccactc tcctcacgtt tagatacgca cgtcattata aaaagcattc gcaataatac
     4441 aatagcaaca aa
//
LOCUS       DATCZV010000622         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_473560, whole genome shotgun sequence.
ACCESSION   DATCZV010000622 DATCZV010000000
VERSION     DATCZV010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473560"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VIW27_13965"
     CDS             <1..287
                     /locus_tag="VIW27_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5740830.1"
                     /translation="WHLGQQASRKRLILLITDGEPADIDERDPQYLRHDAKRAVEELA
                     AGGIHTHCLTLDPQADRYVERIFGPNHYSIVDNVERLPERLPRVFSALTA"
     gene            441..749
                     /locus_tag="VIW27_13970"
     CDS             441..749
                     /locus_tag="VIW27_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5740831.1"
                     /translation="MRNMILGSALALLLLQGPAQAADLAAGESAFSAKGCIGCHGVAG
                     KAPIAPNYPAIGGKDAAFISGELTKFRSGERKEATMNAMAASLSDANIANIAAYLAAQ
                     "
     gene            825..1505
                     /locus_tag="VIW27_13975"
     CDS             825..1505
                     /locus_tag="VIW27_13975"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY5740832.1"
                     /translation="MDFSRIRLVLCDLDGTLVDSVPDLAASANAMLRELGLPERDPQA
                     ARSWVGNGVERFVGRALTGDMDREPDSALLQSALEKFRAHYAANLSRSSTLYPGVEAG
                     LRGLAAAGLHLACVTNKPQPFTSQLIEAMGLAAFFELVVAGDTTARRKPDPMPLQYAA
                     EHFSLHSEQCLMVGDSSNDVVAARAAGFAVACVPYGYNHGADIRDSKPDLVVDNLSEL
                     AERFAGEN"
     gene            1507..>2179
                     /locus_tag="VIW27_13980"
     CDS             1507..>2179
                     /locus_tag="VIW27_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEY5740833.1"
                     /translation="MHLNRREFIRLMGLAGAAGLLPRAGFSAGGDDDRLYDMPAFGNA
                     RILHISDCHAQLNPIYFREPNINIGIGDMRGKAPHLVGQALLDHFAIAAGGIESHAYT
                     YLNFDEAARVYGKIGGFAHLATLVKKMRAEYAEGRSLFIDSGDTWQGSGTAYWTRGQD
                     MVNACNLLGVDIMTGHWEFTYHDTEVLDNVERFAGELVAQNVRVDEVAMFDYELGDRG
                     NFNEDT"
BASE COUNT          413 a          676 c          678 g          412 t
ORIGIN      
        1 gctggcatct gggtcagcag gccagccgca agcggctgat cctgttgatc actgacggcg
       61 agcccgcgga tatcgacgaa cgcgacccgc agtatctgcg ccacgacgcg aagcgcgcgg
      121 tcgaggaact cgcggccggc ggtatccaca cccactgcct gacgctcgac ccgcaggcgg
      181 accgctacgt cgaacgcatc ttcggcccca accattattc catcgtcgat aacgtcgagc
      241 ggctgcccga acgcctgccg cgggtatttt cggcgctgac cgcctgattc agcggcggtt
      301 catggcgaat tcgtaacttt gaaaaaaaac attagtcgat atttccaaag ggttattaca
      361 ttgatcggct gattttgcta gttttgctcc cctggcgaaa ttaaacttgg cttaacacat
      421 tgaattaaag ggggaaatcc atgcgaaata tgattctggg cagcgccctg gcgctattgt
      481 tgctgcaggg cccggcacag gccgccgatc tcgcggcggg cgagagcgcg ttcagcgcca
      541 agggttgcat cggttgtcac ggggttgccg gcaaggcgcc gatagcgccc aattacccgg
      601 ccatcggcgg caaagatgcg gctttcattt ccggcgaatt gaccaagttt cgttccggcg
      661 aacgcaagga agcgacgatg aacgcgatgg cggcttcgct cagtgacgcc aatatcgcca
      721 acatcgcggc ttaccttgcc gcgcagtagg cgggcatcat tcgcgtgacc ccgttgcggg
      781 cagacgattt cagcgacggt cgcgttgccg ggcaggaagg taacatcgat ttttcgcgta
      841 ttcgcctggt cctctgcgac ctcgacggca cgctggtgga cagcgtgccg gatttggccg
      901 ccagcgccaa cgcgatgttg cgcgaactcg gcctgccgga gcgcgacccg caggccgcgc
      961 gctcgtgggt cggcaacggg gtcgagcggt tcgtcgggcg cgcgttgacc ggcgacatgg
     1021 atcgcgagcc tgacagcgcg ctgctgcagt ccgcgctgga gaaattcagg gcccactacg
     1081 ccgccaacct gagccgctcg agtactcttt atccgggcgt cgaagcgggt ttacgcgggc
     1141 tggccgcggc cgggctgcat ctcgcctgcg ttaccaacaa gccgcaaccc ttcacctcgc
     1201 agctgatcga ggcgatgggc ctggccgcat ttttcgaact cgtggttgcc ggcgatacca
     1261 ccgcccgccg caagcccgac ccgatgccgc tgcaatatgc cgccgagcat ttctcgctcc
     1321 acagcgagca atgcctgatg gtgggagact cgagcaacga cgtcgtcgcc gcccgcgccg
     1381 cgggctttgc cgtggcctgc gtgccctacg gttacaatca cggcgccgat attcgcgata
     1441 gcaaacccga cctggtggtc gacaacctta gcgaactcgc cgagcggttc gccggagaga
     1501 attgatatgc acctgaatcg aagagaattt atcagactga tgggactggc aggggccgcg
     1561 gggctgctgc cccgagcggg attctccgcc ggcggcgacg acgatcgcct ctatgacatg
     1621 ccggcgttcg gcaatgcgcg tatcctgcac atttccgact gccacgcgca gctgaacccg
     1681 atttattttc gcgagccgaa catcaatatc ggcatcggcg atatgcgcgg caaggcccct
     1741 cacctggtcg ggcaggcact gctcgatcat tttgctatcg ccgccggcgg tatcgagtcg
     1801 catgcctata cctacctgaa cttcgacgag gctgcgcgcg tctacggcaa gatcggtggc
     1861 tttgcgcatc tcgcgacgct ggtgaagaag atgcgcgccg agtacgccga gggccgcagc
     1921 ctgttcatcg acagcggcga tacctggcag ggctcgggta ccgcctactg gacccgcggc
     1981 caggacatgg tcaatgcctg taacctgctg ggcgtcgata tcatgaccgg gcactgggaa
     2041 ttcacttacc acgacaccga ggtgctcgac aacgtcgagc ggttcgcggg cgaactggtg
     2101 gcgcaaaacg tgcgcgtcga tgaagtcgcg atgttcgatt acgaactcgg cgatcgtggc
     2161 aatttcaacg aagacacgg
//
LOCUS       DATCZV010000623         2413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_156581, whole genome shotgun sequence.
ACCESSION   DATCZV010000623 DATCZV010000000
VERSION     DATCZV010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156581"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VIW27_13985"
     CDS             complement(<1..837)
                     /locus_tag="VIW27_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740834.1"
                     /translation="MSLASRQPTIDSRALHRALQIVAAVIALSTVLLVMRAAPGPIVA
                     EAGDPPHGEHGRQRHPEVFAPLAQDRSTGATLLAGDADTSDLQQFAQPAEPPAAAPTA
                     LPAKQLTAAPLIETFTGLAAIAPKVSSRAAARGAVAAEPPLPKAPPQVATAVPTMEES
                     RPAEPSVIPAADEMVAAPWLAVSPPVAAAAAVREESRPAEPSVIPAAEEMVAAPWLAV
                     SPAVAAAAPVVEQSLPRASARSPVGGETRVEQRLTAPPLALAEDWRAYEEFAPAEPAA
                     PPT"
     gene            complement(1082..1360)
                     /locus_tag="VIW27_13990"
     CDS             complement(1082..1360)
                     /locus_tag="VIW27_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740835.1"
                     /translation="MTDRPSSPLRLLAKTHANALISREQYVAIRARMLKKLEARGYID
                     DSDLSSFTNLAQKSDNQKKTRRSYSISDWIIIGLGLIASAVLAMILYS"
     gene            complement(1357..>2413)
                     /locus_tag="VIW27_13995"
     CDS             complement(1357..>2413)
                     /locus_tag="VIW27_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740836.1"
                     /translation="LDFGIARASTQTDEERENTMFDPSKLSAVTPAYATPEMFAGMNP
                     DPRDDIYGLACVAYQLLAGGKHPYNKVASPKIKELGIKPKPIKGLNRRQQKTLMKALS
                     VVREDRISDVNKFADGMRSRKSHTKTILLATLFLMVIGAGIGYKPFLAFKQEQELYDK
                     IQLIKNGDKALMIETIWSLEQLEPEQQKIISVGLRREIITFYRDRIRAVFRPNEGLYD
                     YPQAQNLLTQAKKHYPDSVALSTIEDQVKEQRDRLMSSLVALYKRYVETRKLVKTPSG
                     EDLTTIMPLIKRVDPKHYLLRDRELADLYLTEAEKLIAQRQYKDASNYIVTGLKMFPE
                     DTRLQSLNKQINAQFRE"
BASE COUNT          402 a          707 c          798 g          506 t
ORIGIN      
        1 agtcgggggc gctgccggct cggccggcgc gaattcctcg tatgcccgcc agtcttcggc
       61 caaggcaagc ggcggcgcgg taagccgctg ctcgaccctg gtttcgccgc cgaccggcga
      121 tcttgcggac gcgcgcggca gggattgctc gacaaccggt gcggccgcgg cgacagccgg
      181 tgacaccgct aaccatgggg cggccaccat ttcttcggcc gcgggaatga ccgagggctc
      241 ggccggtcgc gattcctcgc gcacggcagc ggccgcggcg acgggcggtg acaccgctaa
      301 ccatggggcg gccaccatct cgtcggcagc cggaataacc gaaggttcgg ccggtcgaga
      361 ttcctccatc gttggtaccg ctgtagcgac ttgcggcggc gccttcggca gcgggggttc
      421 ggctgcgacg gccccgcgtg ctgcggcgcg cgacgatacc ttcggcgcga tcgcggcgag
      481 gccggtaaat gtctcgatga gcggtgccgc ggtgagctgt ttcgcgggca aggcggtggg
      541 cgccgccgcc ggcggctcgg cgggctgcgc gaactgctgt aaatcactgg tatctgcgtc
      601 accggcaagc aaggtagcgc ccgtactgcg atcctgcgcc agcggtgcaa aaacttcggg
      661 atgccgctgc cggccatgtt ccccgtgggg tggatcgccg gcctcggcga cgatcggacc
      721 cggcgctgcg cgcatgacca gcagcaccgt cgacagcgcg ataaccgcgg ctactatctg
      781 cagcgctcgg tgcagggccc gagagtcgat ggtcggttgt cgcgatgcaa ggctcatttg
      841 gaattccgct cgaaatcgcc ccacgcactg attcgtgcgt cgcgggcgct tgcaggggtg
      901 ccgatactcc gtcagtcatt cgcaagcaag ccgggcattg cgcgaaatga gttcaggcgg
      961 ctgattaaat gccatccagg atgccgtaga gaatagtaca tcccggagca ggtgcaatcg
     1021 gcagctgtca agaacgtgaa gtgttagagg ttttgccccg gccgcagacc gccgcgagcg
     1081 gctagctgta aagaatcatc gccagtaccg ccgaggcgat cagtcccagg ccgataatga
     1141 tccagtccga tatgctgtac gagcggcgag tttttttctg gttgtccgat ttttgtgcca
     1201 ggttggtgaa ggacgataaa tcggagtcat cgatgtaacc gcgtgcctcg agtttcttca
     1261 gcatgcgagc gcgaatggcg acatactgct cccgggagat cagcgcgttc gcatgggtct
     1321 tggcgagcag gcgcagcggg ctgctgggac ggtccgtcat tcccgaaact gcgcgttgat
     1381 ctgcttgttg aggctttgca gacgggtgtc ttcggggaac attttcaggc cggtcacgat
     1441 gtaattcgac gcgtccttgt actgccgctg cgcgatcagc ttctccgctt cggtcaagta
     1501 cagatcggcc agttcccggt cccgcagcag gtaatgcttg ggatcgacgc gcttgatcaa
     1561 cggcatgatg gtggtcaggt cttcgccgct tggcgtcttt accagtttgc gggtttcgac
     1621 gtagcgcttg tagagggcta ccaggctgct catcaggcga tcgcgctgtt ccttgacctg
     1681 atcttcgatg gtcgacagcg ctaccgagtc ggggtaatgc tttttcgcct gggtcagcag
     1741 attctgcgcc tgcgggtaat cgtacaggcc ctcgttgggg cggaaaaccg cgcgaatccg
     1801 gtcgcggtag aaagtgatga tttcgcgacg cagtccgacc gaaataattt tttgctgttc
     1861 cggctcgagc tgctccagcg accagatggt ttcgatcatc agcgccttgt cgccgttctt
     1921 gatcagctgg atcttgtcgt aaagctcctg ctcctgcttg aaggccagga acggcttgta
     1981 accgatgccg gcgccgatca ccatcaggaa cagcgtcgcc agcaatatcg tcttggtgtg
     2041 cgattttctc gagcgcatgc cgtcggcaaa cttgttgacg tcggagatgc ggtcctcgcg
     2101 gaccacggac aatgccttca tcagggtttt ttgctggcgc cggttgagac ccttgatcgg
     2161 cttgggcttg ataccgagtt ccttgatttt cggcgaagcc accttgttgt aggggtgctt
     2221 gccgccggcc agcaactggt aggccacgca ggccagaccg tagatatcgt cgcgcggatc
     2281 ggggttcatg ccggcaaaca tttccggcgt ggcataagcg ggtgtgaccg cgctcagctt
     2341 ggacgggtcg aacatggtgt tctcgcgttc ctcgtcggtc tgggtgctcg cccgcgcgat
     2401 gccgaagtcg agc
//
LOCUS       DATCZV010000624         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_526891, whole genome shotgun sequence.
ACCESSION   DATCZV010000624 DATCZV010000000
VERSION     DATCZV010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526891"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VIW27_14000"
     CDS             <1..198
                     /locus_tag="VIW27_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5740837.1"
                     /translation="EVVACVVVRDGYQCSEAELKAFCEAGVGKYKCPKAIYFFDDLPK
                     GPSGKILRLKLPELIARGGYR"
     gene            276..1133
                     /locus_tag="VIW27_14005"
     CDS             276..1133
                     /locus_tag="VIW27_14005"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5740838.1"
                     /translation="MSDVSVVGLGAMGSALARTLLDNGYGVTVWNRSTDKAKSVLEAG
                     ALLAESPAAAISASPLTLVCIRSNSDTRRLLEDAGDLAGRGIIDLSTGSAPEARALAE
                     WLGEQGANYLIGMICVFPRDIGDPESTIIAVGEESLWQRSEKLLNCLAGNSSYVGEDI
                     GALAILYSALFLPRQGFMFGMIYGALLCNKAGLPLSTYVEQMPLTIKVVHDYYDVFAS
                     SVLADDFGNPQASIDVYAAAFADTLESFREHGASDELPRLLAELVERGANAGLGDQQV
                     TALINLLNE"
     gene            1334..>2145
                     /locus_tag="VIW27_14010"
     CDS             1334..>2145
                     /locus_tag="VIW27_14010"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY5740839.1"
                     /translation="MLALVLVVTAALAAAFWFENAILRPLSEYLVETATGRTFSIAGE
                     LDARAGRIVTIRATRISLANADWGSRDNLLSIDEVEASVDLLRLLDGTPALDTLEASG
                     IELLFEEDDEGLPNWMMGAGEDNSSTATESRNAIVLPVIRSRFSNVAITFDSAALESP
                     LELRFDSLGHDATQANELRLEAIGAVENRPLNLQARIGPLQQLLGAGAVDFDISADLD
                     VLAFEASGELDSLRAPRQARVKVAAQTADIARIFTTLGLPEMASGASQLAFN"
BASE COUNT          397 a          638 c          711 g          399 t
ORIGIN      
        1 gaagtggtcg cctgcgtggt ggtgcgcgac ggctaccagt gcagcgaggc agaattgaag
       61 gccttttgcg aggccggcgt cggcaagtac aagtgcccga aggcgattta ttttttcgac
      121 gacctgccga agggaccgtc gggcaagatc ctgcgcctga aactgccgga gctgatcgca
      181 cggggcggct atcgctgata cgccatcgcg ggtgattttc accactgccg ggtagtaaac
      241 tgcatgctgt caacgacaaa cccggggagg gcaggatgag cgatgtcagt gtggtcgggc
      301 tcggcgcgat gggatcggcg ctggccagaa ccctgttgga caatggctac ggcgtgactg
      361 tctggaaccg ctcgacggac aaggcaaaat ctgtgttgga ggccggtgcg ctgcttgctg
      421 aaagccccgc ggccgcgatc tccgcgagtc cgctgacgct ggtgtgcatc aggtcgaaca
      481 gcgatacgcg tcgcctgctc gaagacgccg gcgacctcgc cggtcgcggc attatcgatc
      541 tcagcaccgg cagcgcgccc gaggccaggg cgctggccga atggctcggc gagcaaggcg
      601 cgaattatct gatcggcatg atttgcgttt ttccgcgcga tatcggcgac cccgagtcga
      661 ccatcatcgc ggtcggcgaa gaaagcctgt ggcaacgaag cgaaaaactg ctcaactgcc
      721 tggccggcaa ctcgagctac gtcggcgagg acatcggcgc gctggcgatt ctctacagcg
      781 cgctgttcct gccgcgccag ggattcatgt tcggcatgat ctacggcgcg ctgctgtgca
      841 acaaagccgg cctgccgctg tcgacctatg tcgaacagat gccgctgacc atcaaggtcg
      901 tgcacgatta ctacgacgtg tttgcgagct cggtattggc cgacgatttc ggcaatccgc
      961 aggcgtcgat cgacgtctac gcggcggcct ttgccgacac gctggaatcc ttccgcgagc
     1021 atggcgccag cgacgagttg ccgcgcctgc tggcggagct ggtcgagcgt ggcgcgaatg
     1081 ccgggctcgg cgatcagcag gtcactgcgc tgatcaacct gttgaacgaa taattcggct
     1141 agcgtctttt gtctcgcgga tgccgtatga atgtgaacga cgtcagcaag cagtcatcga
     1201 atcgggtcgc cgcctcatcg atgcggaatg gacggctgcg tcgcgggtcg cataggtgat
     1261 ggccgggcgc acacggggcg gggtatcgag ccgcgggcgc gccggccgta tcctggtgtg
     1321 gctgtcgagg ctgttgcttg cgctggtact cgtcgtcacg gccgccctgg cggcggcttt
     1381 ctggttcgaa aatgccatcc tgcgaccgct gtccgagtat ctcgtggaaa cggccaccgg
     1441 ccggacattt tcgatcgccg gtgagctgga cgcccgcgcc gggcgcatcg tcaccattcg
     1501 cgccacgcgc atcagcctgg ccaacgccga ctggggcagc cgcgataatt tactgagcat
     1561 cgacgaggta gaagcctcgg tcgacctgct gcgcttactg gacggcacgc ccgctctcga
     1621 cacgctcgag gccagtggca tcgagctgct gttcgaggag gacgatgagg gcctgcccaa
     1681 ctggatgatg ggggcgggcg aagataattc ttcaaccgcc accgaaagcc gcaatgcgat
     1741 cgtattgccg gttattcgaa gccgattttc caatgtcgcg atcacctttg acagcgcagc
     1801 cctggagtct ccgctcgagc tgcgattcga ctcgctcggg catgacgcga cgcaggcaaa
     1861 cgagttgcgc ctggaggcaa tcggcgcggt agaaaaccgg cccctgaatc tgcaggcccg
     1921 gatcggaccg ctgcagcaac tgctcggcgc gggtgcggta gatttcgata tcagtgcgga
     1981 cctagatgtg ctggctttcg aggccagcgg cgaacttgac agcctgcgcg caccgcggca
     2041 ggcaagagta aaggttgccg cgcaaacggc agacatagcg cgaattttca cgacgctggg
     2101 cctgcctgaa atggccagcg gtgcgagcca actcgctttc aacat
//
LOCUS       DATCZV010000625         2570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_263072, whole genome shotgun sequence.
ACCESSION   DATCZV010000625 DATCZV010000000
VERSION     DATCZV010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263072"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VIW27_14015"
     CDS             <1..582
                     /locus_tag="VIW27_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740840.1"
                     /translation="MLLLVVILGAEAADHHRALGAARIYAMLRRVIVSLALLAALYLC
                     IFIIAAVVAALLLPSMSGGEQSGREQLALLRWIEPGELSFMILFMGTIVTSMWFLAPL
                     LALHELGLRDARGLARRAFDKNDIVVLVASNVPFFTILGCALITQVSVALSPLLVPLF
                     AIYQYVAYRHVFLGRRQNAPLPAKAALGETAFN"
     gene            667..984
                     /locus_tag="VIW27_14020"
     CDS             667..984
                     /locus_tag="VIW27_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740841.1"
                     /translation="MRTLRPTALLAVCLLGPVACDQAVDRASSEYLKYSLRQACGEDD
                     SACIDAVNAQFDDCHANYRRQWDAYIDSASSDEDAMLGVYLRGIYGCIVDKDGQPWFV
                     YDP"
     gene            complement(1017..2279)
                     /locus_tag="VIW27_14025"
     CDS             complement(1017..2279)
                     /locus_tag="VIW27_14025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5740842.1"
                     /translation="MPDSPTARVSRSQAIVAFALGTLFFGYAFVQRVSPSVMTDELMR
                     DFAVGGAALGSLSAFYFYAYASIQLPVGMLTDHFGPRKLMSFAAGLCALASVAFALSD
                     SLLAASIGRALIGATVAFAFVGTLAIAGYWFRPQQYALLAGLLQTVGMTGGIFGQAPL
                     RQLVEGIGWRGSMLVLAAVAIVIGITLLLLVPKRSREQKTHGPQPGIFDGLRAVTANP
                     QTWICSLIGFGMASSMLAFGGLWGVPWLSSVHGYAKTEAAGITSMIFVGWAIFSPLAG
                     WASDRMGRRNPILLGGASITLAALALLVYATPQSTALLMLLIFVIGAGGSSMTVCFGS
                     VRELNDINYSSTSLGLMNMCIVGSGAVMQPLIGWLLDSNWDGQLVDGARVYGAAAYSS
                     AFVSLLVTNAAALAAGLLLRETHCRQHG"
BASE COUNT          461 a          818 c          839 g          452 t
ORIGIN      
        1 atgctgctgc tggtggttat cctgggtgcc gaggctgccg atcatcatcg agccctcggc
       61 gcggcgcgaa tctacgcgat gctgcgtcgg gtgatcgtca gcctggcgct gctggcggcg
      121 ctctacctgt gcatcttcat cattgccgcg gtggtcgcgg cgctactgct gccgagcatg
      181 tccggtggtg agcagtcggg tcgcgaacag ctggccctgt tgcgctggat agagcccggc
      241 gaactgtcgt tcatgatcct gttcatgggc accatcgtta cctcgatgtg gttcctggcg
      301 ccgctgctgg cattgcatga actcggcctg cgcgatgccc gcgggctcgc caggagagcc
      361 ttcgacaaga acgacatcgt ggtgctggtg gcgtcgaacg taccgttttt taccatcctt
      421 ggctgtgccc tgattaccca ggtcagcgtc gcgctgagcc cgttgttggt gccgctgttc
      481 gcgatctacc agtacgtggc ttatcggcac gtatttctcg gccgccggca gaatgcgccg
      541 ctgccggcga aagccgcgct cggcgaaacg gcattcaact gagcggccgt cgccggtctg
      601 ctatagtcat ttggcgactt atcgactcgc cggcggcacg ccgccgctct ggcctggatt
      661 atgccgatgc gaacactccg cccgaccgcg ctactcgcgg tctgcctgct gggcccggtc
      721 gcctgcgacc aagcggttga ccgtgccagc tccgaatatc tgaaatacag cctgcgccag
      781 gcctgcggcg aggacgacag cgcctgtatc gatgcggtga atgcgcagtt cgatgactgc
      841 catgccaact accgccggca atgggatgcc tacatcgact cggcgtccag cgacgaggac
      901 gcgatgctcg gggtttactt gcgcggaatt tacggctgca tcgtcgataa agacggccag
      961 ccgtggttcg tctacgaccc atagcaaatc ggccgccgcg atgcgtcagg acgaatttag
     1021 ccgtgttgcc ggcagtgtgt ctcgcgcaat aacagcccgg cggcgagcgc ggccgcattg
     1081 gttaccagca ggctgacaaa cgcactgctg taggcggcgg caccatagac gcgtgcgccg
     1141 tcgacgagct gcccgtccca gttcgaatcc agcagccagc cgatcagcgg ctgcatgacc
     1201 gcacccgagc cgacgatgca catgttcatc agccccagcg acgtgctgct gtaattgata
     1261 tcgttgagtt cgcgcaccga gccgaagcag accgtcatcg agctgccgcc ggcgccgatg
     1321 acgaagatca gcagcatcag cagcgcggtg ctttgcggcg tcgcgtagac cagcagcgcg
     1381 agcgctgcca gggttatcga tgcgcccccc agcaggatcg gattgcgccg gcccatgcgg
     1441 tccgaggccc agcctgccag cggcgaaaat atcgcccagc cgacgaagat catcgaggta
     1501 atgccggcgg cctcggtctt cgcgtaaccg tgcacgctgg acagccaggg cacgccccac
     1561 agcccgccga aggcgagcat gctcgacgcc atgccaaatc cgatcagcga gcagatccag
     1621 gtctgcgggt tggcggtcac cgcgcgcagg ccatcgaaga tgccgggctg cgggccgtgc
     1681 gttttctgtt cgcgggagcg cttcggcacc agcagcaaca gcgtgatgcc gatgactatc
     1741 gccaccgcgg cgagcaccag catgctgccg cgccagccga tgccttcgac cagttgcctg
     1801 agcggtgcct ggccgaagat gccgccggtc atgccgacag tttgcagcag gccggcgagc
     1861 agtgcgtatt gctgtggtct gaaccagtag ccggctatcg ccagcgtgcc gacgaacgcg
     1921 aaggccacgg tggcgccgat cagcgcgcgg ccgatcgacg cggcgagcag cgaatcgctg
     1981 agcgcgaagg cgaccgaggc cagcgcgcac aggccggcgg cgaagctcat cagcttgcgc
     2041 gggccgaaat ggtcggtgag catgccgacc ggcaactgga tcgatgcata ggcatagaaa
     2101 taaaacgccg acagcgagcc cagcgcggcg ccgccaaccg cgaaatcgcg catcaattcg
     2161 tcggtcatta cgctcggtga gacgcgttgc acgaaggcat agccgaaaaa cagcgtgccc
     2221 agcgcaaagg caacgatggc ttgcgagcgg ctcacgcggg ccgtcgggga atcgggcatc
     2281 ggcttgaatc gtttgcgggt ccagggtcta aattaccacg ttggtgtgca attccaagcg
     2341 atggttcgcc ggcggcggaa aaaccatcgc gatcgcgcgc cacctgtcgc tataatgacg
     2401 cgatgaagta ctggattgcg attgtcatgc tctgggtggc gccggtggcc gtcgccgacg
     2461 ccccgccgaa tccctgttcc gcggagtggt tcgatttcgt cgaacgcata gtcagctccg
     2521 gcgatggcca gggccacggc ccggacctcg gctccgacga gtggcaaggg
//
LOCUS       DATCZV010000626         4959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_527543, whole genome shotgun sequence.
ACCESSION   DATCZV010000626 DATCZV010000000
VERSION     DATCZV010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527543"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VIW27_14030"
     CDS             complement(<1..1247)
                     /locus_tag="VIW27_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5740843.1"
                     /translation="MDAKTDTGRVVSDYDANAADLEREMDWLVKLANRRMAEYFETET
                     GAPAADVIEHPPSLDGTLSGYGRFILDEQLGAEERLLLILTLTPYIRPQLLDVFWSKN
                     TEIDRGFTEFGGVSGSSHGGFLPTLETALFLLTGDRLEERLRLMRRLETGLILVERGV
                     LQIVPAGPQEPWTSGALQISREFVDQIICGANYRPAYGLNFPARRVHTVLDWEHLILP
                     SGTLDQLDEIRNWILYGETLLSEWRMRDKLTPGFTSLFHGPPGTGKTLSACLLGKYCQ
                     RDVYKVDLSLMVSKYIGETEKNLAVIFDAAEHKKWILFFDEADALFGKRTKVSDSHDR
                     YANQEISFLLQRIEEFAGVVILASNFKTNIDDAFIRRFQSVIQFTVPKAGERLQIWRN
                     AFSSHAQLEPGLELARLAEKYEVS"
     gene            complement(1214..2737)
                     /locus_tag="VIW27_14035"
     CDS             complement(1214..2737)
                     /locus_tag="VIW27_14035"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEY5740844.1"
                     /translation="MAGTSHRINRLRLDVEAENAERAFSIRRRLHDDWPRLLNELEAD
                     FDTLAGEGSWLRIPRLHFRIRVANTQDLEQRLPALLHESIGEQLPLLQQSSSAATARD
                     APSDGGLPATVDQRRVAGAEFRPDMPADTRVDRISLEQMLEHYLLYGNLPWFASEPAD
                     WRQRFETLIEDGFESLLARVIEQVSAAPALRLLNLVADAPLLRLLAGWFGERPAASSV
                     QPLLEVMLRSAAREFASRHQRSWVVAALLRQAVRDGGLPAAAAVTEAEWRIPPAYRLP
                     RSRWQALLRRAGAGPNAPLAFGGVCTSAADEEGRSVTGGIETRQARGAQPRSSPVSLE
                     TSPAVEVATQPVRNAGLVLLHPYLARFFEACAIDLRSDRPASEVYDLAAAALYYLAAG
                     EAEPAEYELGLIKILLGLEPSHPLPLGPGLLGEPVKLEAGRLLDSFIGHWSALKSTSR
                     DGLRGAFLQRPALLRDDDDNWLLQVESGGIDVLLERLPFSYSIVKLPWMQKPIQVEW"
     gene            complement(2744..>4959)
                     /locus_tag="VIW27_14040"
     CDS             complement(2744..>4959)
                     /locus_tag="VIW27_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740845.1"
                     /translation="MPTIRTADDAARALDHLVADHVTVSKQITTPAELAARFRGACSS
                     MHGRLAAALPKLWPAAGFLLGDSFSGDPGPGWQSKLASIDSSFAASDTRIQYYFDFLK
                     DLIDTWNEMRDRLFGENAWCCPDISGFPKHLMLGNPVEGGDADENRLGYYPAMAVNRD
                     GRVAEARFLAEKIDKMIGNFSLPAIQNSASVIRVTPSCSARHSLQQRAIPYYYPHVAS
                     NAMVDNWSYRLARRDMNRCNYSYHGDSYAAQGGASEPLASAIDGSDFFRIEGHQGQQA
                     TTVLSRLEQLIAENNLPFSVCGLLLGEQLTDIVDRKGAGYNDLKRLHYLLRQDVVRKL
                     DEVKTFSGSYTAKVHQAVADKKFSNNQESSSAGLTDLADQHNTVIADNSGSAMQWLNQ
                     DYSQYLGKTDWLNNIGHAMAEAGRYKLDLDQVSNTSYNSSFDSWIDYSVVNWLPWLDQ
                     IIGDKDEHADRKKLFGEFVGEHPGLEHTGGVNRGGTFVVVYDESGSVVADFMLSHYYR
                     EDVESPATEPPLDKPQIPKGNLLDDSIWLRPTLDRLLELKLVDFKVDIDKEWQPKLDL
                     QLSYQDLFQDFVNIYAGGVVATPGAIDAGFGNAGGEKLPGGGFTDPMLGLATDRIIQE
                     TQQIEKIYEKLGETTLPADQRTLFESQVARAEENLAKDMEAVARQMDMAGTDVSVGTE
                     GHTTMRAMSKGLQVLDKNTTALNESRTALEGVRSGTGNTGLATNLEKMLGGNFGP"
BASE COUNT          894 a         1546 c         1545 g          974 t
ORIGIN      
        1 gagacttcgt atttttcggc gaggcgcgcc agctccagtc ccggttcgag ctgcgcgtgg
       61 ctggagaacg cgttgcgcca gatctgcaac cgctcgccgg ccttcggcac ggtgaactgg
      121 atcaccgact ggaagcggcg aatgaaggca tcgtcgatat tggtcttgaa gttggaagcg
      181 agtatcacga caccggcaaa ttcctcgatg cgctgcaaca ggaagctgat ttcctggttg
      241 gcgtagcggt cgtgcgaatc gctgaccttg gtgcgcttgc cgaacaacgc atcggcctcg
      301 tcgaagaaca ggatccattt tttatgctcg gcggcgtcga atatgaccgc gagatttttc
      361 tcggtctcac cgatgtactt ggagaccatc aggctcaggt cgaccttgta gacatcgcgc
      421 tggcaatatt tgccgagcag gcaggccgac agcgtcttgc cggtgccggg cgggccgtgg
      481 aacaggctgg tgaaccccgg cgtcagcttg tcgcgcattc gccactccga caacagggtt
      541 tcgccgtaca ggatccagtt gcggatttca tcgagctgat cgagcgtgcc ggacggcagt
      601 atcaagtgtt cccagtcgag taccgtgtgc acgcggcgag cgggaaaatt gagtccgtat
      661 gcgggccggt agttggcacc acaaataatc tggtcgacga actcgcggct gatctgcagc
      721 gcgccgctgg tccacggctc ctgcggcccc gcgggcacga tttgcagtac gccgcgctcc
      781 accaggatca ggccggtttc gagccggcgc atcaggcgca gccgctcctc caggcggtcg
      841 ccggtgagca ggaacagcgc agtttccagc gtcggcagaa agccgccgtg actactgccg
      901 ctgacgccgc cgaactcggt gaatccgcga tctatctcgg tgttcttgct ccagaatacg
      961 tcgagcaact gcgggcggat atacggggtc aaggtgagga ttagcagcag ccgctcctcg
     1021 gcgccgagct gctcgtcgag gatgaagcgg ccgtaaccgg aaagcgtccc gtcgagcgac
     1081 ggcggatgct cgatgacatc ggcggcgggt gcaccggttt cggtttcgaa atactcggcc
     1141 atacgccggt tggcgagttt caccagccag tccatttcgc gctccagatc ggcggcgttg
     1201 gcgtcgtagt cgcttaccac tcgacctgta tcggtttttg catccatgga agtttcacga
     1261 ttgagtaact gaacggcaga cgttcgagca aaacatcgat gccgccggat tcgacctgca
     1321 gcagccagtt gtcgtcgtcg tcccggagca atgccggacg ctgcagaaaa gccccgcgta
     1381 aaccgtcgcg gctggtcgat ttgagggccg accagtgacc gataaagctg tccagcaacc
     1441 ggcccgcctc caatttgacg ggctccccga gcaggcccgg gccgagcggc agcgggtgcg
     1501 acggttcgag tcccagcagt atcttgatca ggccgagctc gtactcggcc ggttcggctt
     1561 cgccggcggc gagataatac aatgccgccg cggccaggtc gtaaacttcg ctcgccggcc
     1621 tgtcggaccg caggtcgata gcgcaggcct cgaaaaaacg cgccaggtac gggtgcagca
     1681 aaaccaggcc ggcattcctc accggctgcg tggcgacttc gaccgccggc gaggtctcga
     1741 ggctgactgg cgaggagcgc ggttgcgcac cgcgagcctg ccgcgtttcg atcccgccgg
     1801 tcacgcttcg tccctcttcg tccgctgcgg aagtacagac gccgccgaag gccagcggtg
     1861 cattcggccc ggcgcccgcc cgccgtagca gggcctgcca gcgagatctc ggcaaccggt
     1921 atgccggcgg aatccgccat tcggcttcgg tcaccgcagc ggcggcaggc agcccaccgt
     1981 cacgcaccgc ctgcctaagc agcgcggcca ccacccagct gcgctgatgg cggctggcga
     2041 actccctcgc tgccgaacgc agcattacct cgagcagggg ttgcacgctg ctcgcagccg
     2101 ggcgttcacc gaaccagccg gcgagcagcc gcagcaacgg cgcatccgcg accaggttca
     2161 gcaacctgag cgccggtgcg gcgctgacct gctcgataac cctggcaagc agcgactcga
     2221 agccgtcctc gatcagtgtt tcgaagcgct gccgccagtc ggcgggttca ctagcgaacc
     2281 atggcaggtt gccgtacaac aggtaatgtt cgagcatctg ctcaaggctg attcgatcga
     2341 cccgggtatc ggcgggcata tccggccgga attcggcccc ggcgacccgc cgttggtcga
     2401 cggtcgcggg taagccaccg tcgctcggtg catcgcgggc cgtggccgcg ctactcgact
     2461 gttggagtag cggcagctgc tcgccgatcg attcgtgtaa cagcgccggc agccgttgtt
     2521 cgagatcctg cgtgtttgcg acccggatgc gaaaatgcag gcgcggaatg cgcagccagc
     2581 tgccctcgcc ggccagcgta tcgaaatccg cttccagttc gttcagcaac cgcggccaat
     2641 cgtcatgcag ccggcggcga atcgaaaaag ctcgttccgc gttttccgcc tcgacgtcga
     2701 ggcgcaagcg attgatacgg tgcgaggtcc cggccatttc gcatcaagga ccgaagttgc
     2761 cgccgagcat tttttcgaga ttggtggcga gcccggtatt gccggtgccg gagcgcaccc
     2821 cttccagtgc cgtgcgtgac tcgttgaggg cggtggtgtt tttgtcgagc acctgcagac
     2881 ccttgctcat cgcccgcatc gtggtatggc cctcggtgcc gacactgaca tcggtaccgg
     2941 ccatatccat ctgcctggcc accgcctcca tgtccttggc gagattttcc tcggcacgcg
     3001 ccacctggct ctcgaaaagc gtccgctgat cggcgggcag ggtcgtttcg ccgagctttt
     3061 cgtatatttt ttcgatctgc tgggtctcct ggatgatgcg atcggttgcc agacccagca
     3121 tcgggtcggt gaacccgccg cccggaagct tttcgccgcc cgcattgccg aaccctgcgt
     3181 cgatggcgcc cggggtagca accacaccgc cggcgtatat gttgacgaaa tcctggaaca
     3241 gatcctgata actgagctgc aggtccagct tgggctgcca ctccttgtcg atatcgacct
     3301 tgaaatcgac cagcttgagt tcgagcaggc gatccagggt cggccgcagc cagatactgt
     3361 cgtcgagcaa gttgcccttc ggaatctgcg gcttgtccag tggtggttcg gtcgcggggg
     3421 attcgacgtc ctcccggtag tagtgtgaca gcatgaagtc ggccaccacg ctgccgcttt
     3481 cgtcgtatac gacgacgaaa gtcccgcccc gattgacgcc gccggtatgc tcgagtcccg
     3541 ggtgctcgcc gacgaattcg ccgaacagct ttttgcgatc ggcgtgctcg tccttgtcgc
     3601 cgataatctg gtccagccac ggcagccagt tgacgacgga gtaatcgatc cacgaatcga
     3661 aagacgagtt ataggaggtg ttcgaaacct ggtccaggtc gagcttgtag cgacccgcct
     3721 cggccatcgc atggccgata ttgttcagcc agtcggtttt gccgagatac tgggaataat
     3781 cctggttcag ccactgcatc gcgctgccgc tgttgtcggc tattaccgtg ttgtgctggt
     3841 cggcgagatc ggtgaggccg gcactgctcg attcctggtt attgctgaat ttcttatcgg
     3901 ccaccgcctg gtgcaccttg gcggtatagc tgccactgaa ggttttgact tcgtcgagct
     3961 tgcgcaccac gtcctgccgc aacaggtaat gcaggcgttt cagatcgtta taaccggcac
     4021 ccttgcgatc gacgatatcg gtcaactgct cgccgagcag caagccgcag acgctgaacg
     4081 gtagattatt ttccgcgatc aactgttcga gccgactcag aaccgtggtc gcctgttgcc
     4141 cctggtgccc ttcgatacgg aaaaaatcgc tgccatcgat cgcgctggcc agcggttcgg
     4201 aagcaccgcc ctgggcggcg tagctgtcgc catgatagga atagttgcaa cgattcatat
     4261 cgcgccgcgc aagccgatag ctccagttat cgaccatcgc gttgcttgcc acgtgagggt
     4321 aatagtaggg aatcgcacgc tgctgcaggc tgtgcctggc gctgcaactc ggcgtaacgc
     4381 gaatcacgct ggccgaattc tgtatcgccg gcaacgaaaa attaccaatc atcttgtcga
     4441 tcttttcggc gaggaagcgg gcctcggcaa cgcggccatc ccggtttacc gccatcgcgg
     4501 ggtaatagcc gagccgattt tcgtcggcat cgccgccctc gaccgggttg ccgagcatca
     4561 ggtgcttggg gaatccgctg atatcgggac aacaccacgc gttttcaccg aacagccgat
     4621 cgcgcatttc attccaggta tcgatgaggt ccttgaggaa atcgaaatag tactggatgc
     4681 gcgtatcgct ggcggcaaag ctgctgtcga tgctcgccag cttcgactgc cagcccgggc
     4741 ccgggtcgcc ggaaaagctg tcgccgagca ggaacccggc cgcgggccag agcttgggca
     4801 acgcagcggc gaggcgcccg tgcatgctgc tgcaggcgcc gcgaaagcgc gcggcgagct
     4861 ccgccggggt cgtgatctgc ttggagaccg tgacgtgatc ggccaccagg tgatccaacg
     4921 cgcgcgccgc atcgtccgcc gttcgaatcg tcggcatca
//
LOCUS       DATCZV010000627         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_474881, whole genome shotgun sequence.
ACCESSION   DATCZV010000627 DATCZV010000000
VERSION     DATCZV010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474881"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VIW27_14045"
     CDS             <1..475
                     /locus_tag="VIW27_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740846.1"
                     /translation="LMRVPRQRQSAVHESQAPPGFFLSCCRQRDERASSPVIDGDDLL
                     ENPHGIVEAYCNVAGIPCIEAALSREPGSRDEISCQDGDSWHANLRDSDGFKPQPRRY
                     IDDSKAPSVEGNLRDLAAPLRAPSRATTRRDLRNCRLTANPSVRKPQLCCTMVLR"
     gene            complement(445..1065)
                     /locus_tag="VIW27_14050"
     CDS             complement(445..1065)
                     /locus_tag="VIW27_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5740847.1"
                     /translation="MYTLFWEKGSGSIVVQAVLEEMGIPYDLRYVDMQAGENRQSAYL
                     AENPTGLVPALKLPDGRTIGESAAIVLYLGEQGSEGQVLPPPGDAERADFLYWLLYMA
                     TMGYKNFGRFAHPERYTLDQDALDVVSGAARNDITDFFDVVDAAIGGQPYFLPSGFSA
                     LDIYLAMLAGWHPNRDELFERNPRFAALCSAVESRPTYRKTMVQHS"
     gene            1192..2142
                     /locus_tag="VIW27_14055"
     CDS             1192..2142
                     /locus_tag="VIW27_14055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5740848.1"
                     /translation="MKTSHLNNLRALEATLRTGSFRAAAEELGVTPAAVGQQVRGLEN
                     FLDRKLLRRSTAGVTPTDAAQRVQHRLTTSFLAIEEIIGLLKNRQAGSRVAITLPSSF
                     AENWFTGRLADFYRLNSNIDLRLDASNRMVDLAGEDFDFAIRYSEPSSAVYDDTELFG
                     DHVLPLCTPDFASRHGLSMRTKSLRGIPLVHLDSRTTDPDWADWTRWGEAFDLDGDGL
                     QDGIRLTEFNSGLQAAIRGQGLVLCGVVEAFNALGDGTLVAPFGAARCCPAGYAYRLV
                     AIRGRDLSPLQIQFRDWIIAVTGEFRGQLNAFVSPGANPD"
BASE COUNT          450 a          735 c          669 g          473 t
ORIGIN      
        1 tctgatgcgc gtgccgcgcc agcggcaatc cgcggttcac gaaagccagg ccccgcctgg
       61 ctttttttta tcctgctgcc ggcagcggga cgagcgcgca tcgtcgccgg tgatcgacgg
      121 cgacgatttg ctggaaaatc cgcatggcat cgtcgaggct tactgcaatg tggcaggtat
      181 tccctgcatc gaggccgcgc tctcccggga gcctggttcg cgcgacgaga tcagctgcca
      241 agacggtgac tcctggcatg ccaacctgcg cgactcggac gggttcaagc cccagccgcg
      301 ccgctacatc gacgactcga aggcaccgtc ggttgaaggt aatctacgag accttgctgc
      361 ccctctacga gcaccttcac gcgcaacgac ccgccgcgac ttacgaaact gccggctgac
      421 cgcgaacccg tctgtccgga agcctcagct gtgttgcacc atggttttgc gataggtcgg
      481 acggctctcg acggcactgc agagtgcggc aaatctcgga ttgcgctcga acagttcgtc
      541 gcgattcgga tgccatcccg cgagcatcgc caggtaaatg tcgagtgcgc tgaagcccga
      601 tggcaggaaa taaggctgac cgccgatcgc cgcgtccacc acgtcgaaaa agtcggtgat
      661 atcattacgc gccgcaccgc tgacgacatc gagcgcatcc tgatccagtg tgtagcgctc
      721 gggatgcgca aatcggccaa agttcttgta gcccatggtt gccatgtaca gtagccagta
      781 aagaaaatcg gctcgctccg catcgcccgg aggaggcaac acctggccct cgctgccctg
      841 ctcgccgaga tacagcacga tagctgccga ttcgccgatg gtgcgcccgt cgggcagttt
      901 cagagccggc accaggccgg tcgggttttc cgccaggtag gcgctctgcc gattttcacc
      961 cgcctgcatg tcgacgtacc tcaggtcgta cggtataccc atttcctcga gtaccgcctg
     1021 tacgactatc gaacccgaac ctttttccca gaaaagcgtg tacatgtgac tcccccaaat
     1081 aaagctaata tttatattta acaataccag tctgcatata taagctataa atgacttact
     1141 ctcagcgcat ttattctttt tttatataaa gattatcttt acactttgcg aatgaagacc
     1201 agccacctga acaatctcag ggcgctcgag gcgaccctca gaacaggcag ttttcgtgcc
     1261 gcggccgagg aactgggcgt gaccccggcc gcggtcggtc agcaggtgcg cggactggaa
     1321 aacttcctcg atcgcaagct gttgcggcgc agcaccgcgg gcgtcacgcc aaccgacgcc
     1381 gcgcagcggg tacagcaccg cctgaccacg agttttctcg ccatcgagga aataatcggc
     1441 ctgctgaaaa accggcaggc gggcagccgc gtcgcgatca ccctgccttc gtcgttcgcc
     1501 gaaaactggt ttaccggccg gctcgcggat ttctaccggc tgaactcgaa catcgacctg
     1561 cggctcgacg ccagcaaccg catggtggac ctggccggcg aggatttcga cttcgcgatt
     1621 cgttatagcg aaccgtcgtc ggctgtttac gacgatacgg aactattcgg cgatcatgtg
     1681 cttcccctgt gcacgccgga ttttgcctcc cggcacggct tatcgatgcg cacgaagtcc
     1741 ctgcgcggga ttcctctcgt ccatctcgac agccgcacca cggatccgga ctgggccgac
     1801 tggacgcgtt ggggcgaggc tttcgatctc gacggcgacg gcttgcaaga cgggattcgc
     1861 ctgaccgagt tcaattcggg tctgcaggcg gcaatccgcg gacagggcct ggtgctgtgc
     1921 ggcgtcgtcg aggcgttcaa cgctctcggc gatggcaccc tggtggcacc tttcggcgcg
     1981 gcacgctgct gcccggccgg ttacgcttac cgcctggtcg ccatccgcgg gcgggatttg
     2041 tcgccactac aaatccagtt ccgcgactgg atcatcgctg tcaccggcga atttcgcggc
     2101 cagttgaacg ccttcgtctc gcccggcgcc aatcccgact gagcattttt cacgcggtaa
     2161 ttgatccagg tcggcaattc cgctcgcgcg cgggtgtatg ttgaatagcg atccattcaa
     2221 aggaggattc gaagatgtcc atactcgtct tggtcgccga tggcagccgg gcgcgattcc
     2281 tgtacgcgga agacggcggc tcgacactca ccgaaaccga ggatctc
//
LOCUS       DATCZV010000628         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_740439, whole genome shotgun sequence.
ACCESSION   DATCZV010000628 DATCZV010000000
VERSION     DATCZV010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740439"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VIW27_14060"
     CDS             complement(<1..392)
                     /locus_tag="VIW27_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY5740849.1"
                     /translation="MPEISRSALLPYSPEFVYDIVNGVEAYPDFLPWCGDSRVEQQSD
                     TEMQAAIQIRRAGIRQWFRTHNRMQPGRSIDMELVDGPFRQLHGQWKFRPLDSRGCKI
                     ELVLAFEFKAGLASTLIGPAFTRIANTMV"
     gene            complement(394..1791)
                     /locus_tag="VIW27_14065"
     CDS             complement(394..1791)
                     /locus_tag="VIW27_14065"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY5740850.1"
                     /translation="MSSTENAFTWRSRSAFVIVAAGATLGLNDFLTFPVLAVHNGGGA
                     FLILYILFLFVLGLPLLMGELLLGRLTRSDPSDCLRILAEQHKASVYWDLVGLASMFA
                     AFLILSTFSVVAGWSLAYTVRAAIGMFNGVTLEDARLLFQALTFDSERMALWHTLFVV
                     LLVAISAQPPARGIERAMLVLVPTMVLLLLIGLLVALFSGGMATSIASILHADFGAID
                     AHTPLLALQRAFYTLALGLGVMIALGRYLPENIPLGYSAALVVTVDLLFSIVTGLSIN
                     ALLFSAGQEPGIDSQFAFRIMPVILDRFSSGSLFTTLFFMLMAIASLTTSIALIEAPI
                     TYLERRRGYSRLRAATYLGIAIWLCGLGVVLAHSVWNGDGFSLALFFDEKTVRLVNNA
                     GFHDVLVFISSHLIQPLAALFLCLFVAWKIPRAVSHQTLGLSRHYNFEIWCYLLRYIV
                     PVLMLVVILAAFGII"
     gene            complement(1942..2685)
                     /locus_tag="VIW27_14070"
     CDS             complement(1942..2685)
                     /locus_tag="VIW27_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5740851.1"
                     /translation="MINILTYNIHKGFDRYNRDFVLHQIKENLHDTEVDIVLLQEIQG
                     RHLHHETRISSWPDVSQFEFLADSVWPHYAYGKNAIYSKGHHGNAILSKYHIAEWNNL
                     NLSRFQRASRSLLHGRVELPGGKRPLHLLCVHLDLLGFERKRQLRELRQYIDSSIDDG
                     SPVILGGDFNDWRGGLGKLLESQLGMQEAYRKLNGRYARTFPSHWPMLCMDRIYFRDI
                     ELMDAHCMQRHPWDQLSDHLPLYARFAVD"
     gene            complement(2714..>2948)
                     /locus_tag="VIW27_14075"
     CDS             complement(2714..>2948)
                     /locus_tag="VIW27_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY5740852.1"
                     /translation="GPGELRVFVSGMISREGNAEIIRKMQYLAKEMNELSVAAESLPL
                     EQRFGSSLMLAVRPWEIGVFTALRRTPDERIHG"
BASE COUNT          646 a          851 c          917 g          534 t
ORIGIN      
        1 accatggtgt tggcgattcg cgtaaacgcc ggtccgatca gggtcgacgc gaggcccgcc
       61 ttgaattcga atgccagcac cagttcgatc ttgcagccgc ggctgtcgag tggtcgaaat
      121 ttccactggc cgtgcaactg ccggaacgga ccgtcgacga gttccatgtc gatcgatcgg
      181 cccggttgca tacggttgtg ggtcctgaac cattgcctga tcccggcgcg acgaatctgg
      241 attgccgcct gcatctcggt gtcgctttgc tgttcgaccc tggaatcgcc acaccacggc
      301 agaaagtcgg gatacgcctc gaccccgttg acaatgtcgt agacgaattc cggtgagtaa
      361 ggcagcagcg cgctgcgcga gatttcgggc acttcagatg attccaaatg cggccagtat
      421 aacaaccagc atcagcaccg ggacaatata tctcagcaga tagcaccaga tttcgaaatt
      481 gtagtggcgc gacaggccca gggtctggtg actgaccgcg cgcggaatct tccaggcgac
      541 gaacaggcac aggaacagtg cagccagcgg ctggatcagg tggctggaga taaacaccag
      601 cacgtcgtga aagccggcgt tgttgaccag gcgcacggtt ttttcgtcga agaacagggc
      661 cagcgagaaa ccatcgccgt tccacacgct gtgcgccagc accacgccga gcccgcacag
      721 ccagatagcg atgccgagat aggtcgcggc gcgcagccgc gaatatccac gcctgcgctc
      781 gagataggtg atcggcgcct cgatcagggc gatcgaggtg gtcagcgagg cgatcgccat
      841 cagcatgaag aacagcgtgg tgaacaggct gccggatgaa aaacgatcga gaatcaccgg
      901 catgatgcgg aatgcgaact ggctgtcgat tccgggctcc tgcccggcgg agaacagcaa
      961 cgcgttgatc gacaggccgg tgacgatcga gaacagcaga tcgacggtca ccaccagcgc
     1021 cgccgagtaa cccagcggga tgttttccgg caggtaacgg cccagcgcga tcatcacgcc
     1081 gaggccgagc gcgagtgtat aaaaggcccg ctgcagcgcg agtaacgggg tatgggcgtc
     1141 gatggcaccg aagtcggcat gcaggatcga ggcgatactg gtggccatgc cgcccgagaa
     1201 cagcgccacc agcagcccga tcagcagcaa cagcaccatc gtcggcacca gcaccagcat
     1261 cgcgcgctcg atgccgcgcg ccggtggttg cgcgctgatc gccaccagca gcaccacgaa
     1321 aagtgtatgc cacagtgcca tgcgctcgct gtcgaaggtc agtgcctgga acaacaggcg
     1381 ggcatcctcg agggttacgc cgttgaacat gccgatcgcc gccctcaccg tataggcgag
     1441 cgaccagccg gcgaccacgc tgaacgtgga taggatcagg aaggccgcaa acatcgaagc
     1501 caggccgacc agatcccagt agaccgatgc cttgtgctgc tcggcgagga ttctcaggca
     1561 atcgctggga tcgctgcggg tcagacgccc gagcagcagt tcgcccatca gcagcggcaa
     1621 gccgagaacg aacaggaaca ggatatagag gatcaggaac gcgccgccgc cattgtgcac
     1681 cgcaagtacc ggaaaggtca gaaaatcgtt gagacccagg gttgcgccgg cggcgacgat
     1741 gacgaacgcc gatcgcgacc gccaggtgaa cgcgttttca gtgctgctca tagccttccg
     1801 ttgcagccgg gctgcggatt aacagggagc caagtctagc aagaaagacc tgtcgtctaa
     1861 acacaataaa ttcaatggct tagttagtat agttaaggcc ggttattctg caattgccga
     1921 attcctcgcg gcgccgggaa gtcagtcgac ggcgaagcgc gcgtaaagcg gcaaatgatc
     1981 cgaaagttga tcccagggat ggcgctgcat gcagtgcgca tccatcagtt cgatgtcgcg
     2041 aaaatagatt cgatccatgc acagcatcgg ccagtgcgac gggaaggttc gagcgtagcg
     2101 gccgttgagc ttgcggtagg cttcctgcat gccgagttgc gattcgagca gcttgccgag
     2161 tcctccgcgc cagtcgttga aatcgccgcc cagaatcacc gggctgccgt cgtcgatcga
     2221 cgaatcgata tattgccgca gttcgcgcaa ctggcgcttg cgttcgaaac cgagcaggtc
     2281 gagatgcacg cacagcaggt gcagcggtcg cttgccaccg ggcagttcca ccctgccgtg
     2341 cagcaggctg cggctggccc gctggaagcg ggacaggttg agattgttcc attcggcgat
     2401 atggtacttg ctgaggatcg cgttgccgtg atgacccttg ctgtaaatgg cgttcttgcc
     2461 ataggcatag tgcggccaca cgctgtcggc gaggaactcg aactggctga cgtcgggcca
     2521 gctggagatg cgggtttcat ggtgcaaatg gcgaccctgg atttcctgca gcagcacgat
     2581 atcgacctcg gtgtcgtgca ggttttcctt gatctgatgc agtacgaaat cgcggttgta
     2641 gcgatcgaag cccttgtgaa tgttgtaggt gagtatattg atcattcaat gacgccgtgg
     2701 gttgactgcg tattcaacca tggatgcgct cgtccggcgt gcgccgcaac gcggtaaaaa
     2761 cgccgatttc ccaggggcgc acggccagca tcaggctgct accgaaacgc tgctcgagcg
     2821 gcagcgattc ggcggcgacg ctcaactcgt tcatttcctt cgccaggtac tgcatcttgc
     2881 ggatgatttc ggcattgcct tcgcgagaga tcatgcccga cacgaaaact ctcagttcgc
     2941 cggggccg
//
LOCUS       DATCZV010000629         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_422271, whole genome shotgun sequence.
ACCESSION   DATCZV010000629 DATCZV010000000
VERSION     DATCZV010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422271"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..2216
                     /gene="parC"
                     /locus_tag="VIW27_14080"
     CDS             18..2216
                     /gene="parC"
                     /locus_tag="VIW27_14080"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY5740853.1"
                     /translation="MFTEKAYLDYSMYVILDRALPHIGDGLKPVQRRIIYAMSELGLS
                     ASSKHKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLIDGQGNFGSPDDPK
                     SFAAMRYTESRLSEIARLLLQELGQGTVDWVANFDGTMDEPSLLPARLPHILLNGTTG
                     IAVGMATDMPPHNLREIAEACVMLLDKPSTALKDLRSKVLGPDYPTEAEIITTPAELD
                     NLYESGGGSIRMRAVYEREDGDIIVTALPHQVSGARIMEQIAGQMLAKKLPMVDDLRD
                     ESDHENPTRLVIVPRSNRVDVDALMGHLFATTDLERTYRVNMNMIGTNGRPQVKNLKQ
                     ILQEWIAFRIATVRRRLQWRLDKVEARLHLLEGLLIAFLNLDEVIRIIREEDEPKKKL
                     IKRFKLSDLQAEEILNTRLRHLAKLEEMKLNAEQQELMDEKAELDKLLGSERRIKTLI
                     KKEIQADAETYGDQRRSPIVARQAARALDETDLMPSEALTVVLSSKGWVRAAKGYDVD
                     GRGLSYKSGDGFQDMAQGKSNQSVVFVDSNGRAYSLAAHSLPSARGQGEPLTGRFKPA
                     GGAEFRSCMMGRDDDLFLISSSHGYGFICRFTDMVTRNKNGKAQISLQKGAQLMPVVA
                     VTDYQQDWLVSISSDGYIAVIDAAQVPRLAKGKGNKIHGIPPKRLKAGDEQIAFIACL
                     TGRQKLVIHCGKKYKTMNQNELEAYRIERGKRGRKLPRGYQNVTAIEVAD"
     gene            2288..3172
                     /gene="htpX"
                     /locus_tag="VIW27_14085"
     CDS             2288..3172
                     /gene="htpX"
                     /locus_tag="VIW27_14085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY5740854.1"
                     /translation="MASRILLFLLANFGVLAVLSVSMRLLGVDAYLASQGGAFQLNGL
                     LIMAAIIGFAGSFISLAMSKTMARRAMGVQIIEQPRGEAERWLLAVVRRQAEAAGIGM
                     PEVGVFESPTMNAFATGMNRDDALVAVSTGLLAGMSRDEVEAVLGHEVSHVANGDMVT
                     MGLLQGVLNTFVIFFSRVIGIVIDRTVFRIERGVGPGFWIGSIVAEIVLGIVAAMIAA
                     WFSRRREFRADAGGAQLAGRGKMIAALDRLRRGQGQSDLPGEIASFGISGKVGLAALL
                     ATHPPLELRIERLRADSD"
     gene            complement(3187..>3641)
                     /locus_tag="VIW27_14090"
     CDS             complement(3187..>3641)
                     /locus_tag="VIW27_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740855.1"
                     /translation="HRSLARSLSAYFADSRLLHIVTAPLIYSCILPALLMDLVVTVYQ
                     AVCFRVYGIPRVERGDYIVIDRNQLQYLNAIEKMNCVYCGYFNGFIAYVGEIAARTEQ
                     YWCPIKHARKVAAMHSRYHKFIEYGDSEDYQNRLTALRREFDDLRSNG"
BASE COUNT          699 a         1123 c         1165 g          654 t
ORIGIN      
        1 agcagcagcc gctgcacgtg tttaccgaga aggcttacct cgattattcg atgtacgtga
       61 ttctcgaccg tgcgctgccg cacatcggtg acggcctgaa gccggtacag cggcgcatca
      121 tctacgcgat gtccgaactc ggcctgtcgg cgagttccaa gcacaagaag tcggcgcgca
      181 ccgtcggcga cgtgctcggc aagtatcacc cgcatggcga cagtgcctgt tacgaggcga
      241 tggtgctgat ggcgcagcct ttttcgtacc gctacccgct gatcgacggc cagggcaatt
      301 tcggctcacc cgacgatccc aagtcattcg cggcgatgcg ctataccgag tcacggttgt
      361 cggaaatcgc acgcctgttg ctgcaggagc tgggacaggg caccgtcgac tgggtcgcca
      421 acttcgatgg caccatggat gagccgtcgc tgctgcccgc gcggctgccg catatcctgt
      481 tgaacggcac caccggcatc gcggtcggca tggcgaccga tatgccgccg cacaacctgc
      541 gcgagatcgc cgaagcctgc gtgatgttgc tcgacaaacc gagcaccgcg ctcaaggatt
      601 tgcgcagcaa ggtactcgga ccggattacc cgaccgaggc cgagatcatc accacgcccg
      661 cggaactcga caatctctac gaaagcggcg gcggctcgat tcgcatgcgc gccgtctacg
      721 aacgcgagga cggcgatatc atcgtcaccg cgctacctca ccaggtgtcg ggggcgcgga
      781 tcatggagca gatcgcgggc cagatgctgg ccaagaagct accgatggtc gatgatctgc
      841 gcgacgaatc ggaccacgaa aacccgaccc ggctggtgat cgtgccgcgc tccaatcggg
      901 tcgatgtcga cgcgctgatg ggccacctgt tcgcgaccac cgacctcgag cgcacctatc
      961 gcgtcaacat gaacatgatc ggcaccaacg gccgcccgca ggtcaagaac ctgaagcaga
     1021 tcctgcagga atggatcgcg tttcgcatcg ccaccgtgcg ccgccgcctg caatggcggc
     1081 tggacaaggt cgaggcgcgg ctgcacctgc tcgaaggcct gctgatcgcg ttcctcaatc
     1141 tcgacgaggt gattcgtatc attcgcgagg aagacgagcc gaaaaagaaa ttgatcaagc
     1201 gcttcaagtt gagcgacctg caggctgagg aaatcctcaa tacccgcctg cgccacctgg
     1261 ccaaactcga ggaaatgaag ctcaacgccg agcagcagga actgatggat gaaaaggccg
     1321 aactcgacaa gctgctgggc tcggagcgtc gcatcaaaac gctgatcaag aaggaaatcc
     1381 aggccgacgc cgagacttac ggcgaccagc gccgttcgcc gatcgtcgcg cggcaggctg
     1441 cacgcgcgct cgacgagacc gacctgatgc cgtcggaggc gctgaccgtg gtgctgtcga
     1501 gcaagggctg ggtgcgcgct gccaagggct atgacgtcga tgggcgcggc ctgtcctaca
     1561 agtcgggcga cggcttccag gacatggcgc agggcaagag taaccagagc gtggtgttcg
     1621 tcgattccaa cgggcgcgcc tattcgctgg ccgcgcacag cctgccgtcg gcgcgcggcc
     1681 agggcgagcc gctgaccggg cgtttcaagc ccgccggcgg tgccgagttc aggagttgca
     1741 tgatggggcg cgacgacgac ctgttcctga tatcgtcgtc gcacggttac ggctttatct
     1801 gccgcttcac cgatatggtg acacgcaaca agaacggcaa ggcgcagatc agcctgcaaa
     1861 aaggtgcgca actgatgccg gtggtcgcgg tcaccgatta ccagcaagac tggctggtgt
     1921 cgatcagcag cgatggctat atcgccgtga tcgacgccgc gcaggtgccg cggctggcga
     1981 agggcaaggg caacaagatt cacggtattc cgccgaagcg cttgaaggcc ggcgacgagc
     2041 aaatcgcctt cattgcctgc ctgaccggcc gccagaagct cgtcattcac tgcggcaaga
     2101 aatacaagac gatgaaccag aatgaactcg aggcttaccg catcgaacgc ggcaagcgcg
     2161 gccgcaagct gccgcgcggc taccagaacg tcaccgcgat cgaggtcgcc gactgagcct
     2221 gccgacttga tttcttatct gatgacccca atttccaagc gggtttaagc atacttacgg
     2281 agctctgatg gcttcaagaa tcctgttgtt cttattggca aatttcggcg tgctcgccgt
     2341 actcagtgtt tccatgcgcc tgctcggtgt ggatgcctat ctcgccagcc agggcggcgc
     2401 tttccagctc aatggcctgc tgatcatggc cgcgatcatc ggcttcgccg gctcgttcat
     2461 ttcgctggcg atgtcgaaga cgatggccag gcgcgcgatg ggcgtacaga tcatcgaaca
     2521 gccgcgcggc gaggccgagc gctggctgct ggcggtggtg cgccgccagg ccgaggcagc
     2581 cggtatcggt atgcccgagg tcggcgtctt cgagtcgccg acgatgaacg cctttgccac
     2641 cggtatgaac cgtgacgatg cgctggtcgc ggtcagcacc ggcctgctcg ccggcatgtc
     2701 gcgcgacgag gtcgaggcgg tgctcggcca cgaggtgtcg cacgtcgcca acggcgatat
     2761 ggtgaccatg ggcctgctgc agggcgtgct caataccttc gtgattttct tttcgcgcgt
     2821 catcggcatc gtcatcgacc gcacggtgtt ccgtatcgag cgcggcgtcg gtccgggctt
     2881 ctggatcggt tccatcgtcg ccgaaatcgt gctcggcatc gtcgccgcga tgatcgccgc
     2941 ctggttttcg cgacgccgcg agttccgcgc cgatgccggc ggcgcacagc tcgccggccg
     3001 cggcaagatg atcgccgcgc tggatcggct gcgccgcggc caggggcagt cggacctgcc
     3061 gggtgaaatt gccagtttcg gcatcagcgg caaggtcggg ctggccgcgc tgctggcgac
     3121 ccatccgccg ctggagctgc gcatagagcg cctgcgggcg gactccgact gagcgcgaaa
     3181 cgcggctcag ccgttgctgc gtaaatcgtc gaactcgcgc cgcagcgcgg tgaggcggtt
     3241 ctggtaatcc tcgctgtcgc cgtactcgat gaacttgtgg tagcgactgt gcatcgccgc
     3301 gaccttgcgg gcatgcttga tcggacacca gtattgctcg gtgcgcgcgg cgatttcgcc
     3361 cacgtaggcg atgaatccgt tgaagtagcc gcagtagacg cagttcattt tctcgatcgc
     3421 gttgaggtat tgtaactggt tgcggtcgat gacgatgtaa tcgccgcgct cgacgcgcgg
     3481 aatcccgtac acgcgaaagc agaccgcctg gtacacggtc accaccaggt ccatcagcag
     3541 cgcgggcaga atgcaggaat agatcagcgg tgcggtaacg atatgcaaca gtcgcgaatc
     3601 ggcgaagtag gcgctcagcg atctcgccag gctacgatga t
//
LOCUS       DATCZV010000630         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_316485, whole genome shotgun sequence.
ACCESSION   DATCZV010000630 DATCZV010000000
VERSION     DATCZV010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316485"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VIW27_14095"
     CDS             <1..675
                     /locus_tag="VIW27_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5740856.1"
                     /translation="FTRLIREVVVAAKAGGSDLDANPSLRTAVDKAKSQSVPKDAIER
                     AVKRGAGELEGSDYEEIRYEGYGPAGVAFIVDCLSDNRNRTVAEVRHAFSKAGGNLGQ
                     SGSVAYLFSSVGVLLYSPEHDEDSVMEAALDAGAEDVIVADDGSIEVLTAAEEYAAVK
                     QAMTDAGLEPEESDLTMRASTNTEVDLETAGKLLRLVDLLEASDDVQEVYYNAEIPEE
                     AYEAEG"
     gene            675..1199
                     /gene="ruvC"
                     /locus_tag="VIW27_14100"
     CDS             675..1199
                     /gene="ruvC"
                     /locus_tag="VIW27_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5740857.1"
                     /translation="MIILGIDPGSRITGFGLIDNHANRMEYIHSGSIKVSGDSLPQRL
                     GYIFAEIETVIRQYRPQQMGIENVFMARNADSALKLGQARGAAICAGHQAGLEISEYA
                     AREIKQAIVGTGAASKDQVQHMVKRLLGIRHELQADEADGLAIAICHAQFYATRQKTG
                     LDSSQLKRRRSRRR"
     gene            1196..1798
                     /gene="ruvA"
                     /locus_tag="VIW27_14105"
     CDS             1196..1798
                     /gene="ruvA"
                     /locus_tag="VIW27_14105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5740858.1"
                     /translation="MISRLTGILAAKRAPQILIDCHGVGYEADVSMTTFYKLPEVGQQ
                     ISIWTHLLVREDSHSLIGFDNEQERKLFRQLIRITGVGPKMALTILSGIDEQQFALCI
                     ANNDIAMLTRLPGVGKKTAERLIIEMRDKIDVTAGDLPTQQAAAGGHSIVNEAIEALQ
                     ALGYKPIDAEKMISRAQQQGESADSASQLIKQALQTTVKI"
     gene            1795..>2261
                     /locus_tag="VIW27_14110"
     CDS             1795..>2261
                     /locus_tag="VIW27_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5740859.1"
                     /translation="MSEERLISAEAVEEELIQDRALRPKMLADYVGQEEVREQMEIFV
                     QAAVQRREALDHVLIFGPPGLGKTTLSHIIANEMQVNLRQTSGPVLEKAGDLAAILTN
                     LEAHDVLFIDEIHRLSPVVEEILYPAMEDFQLDIMIGEGPAARSIKLDLPPFTL"
BASE COUNT          510 a          663 c          688 g          400 t
ORIGIN      
        1 ttcacccgcc tgattcgtga agtcgtcgtc gccgccaagg ccggcggttc cgatctcgac
       61 gcaaatccat cgctgcgcac cgcggtcgac aaggccaagt cgcaatcggt gccgaaggac
      121 gcgatcgagc gcgcggttaa acgcggcgcc ggcgagctcg agggctccga ctatgaagaa
      181 attcgctacg agggttacgg tccggccggg gtcgccttca tcgtcgactg cctgtcggat
      241 aatcgcaatc gcaccgtggc cgaggtgcgc cacgccttca gcaaggccgg cggtaatctc
      301 gggcaaagcg gttcggtggc ttacctgttt tccagcgtcg gcgtcttgct gtactcgccc
      361 gagcacgacg aggacagcgt gatggaagcc gcgctcgacg ccggcgccga ggatgtgatc
      421 gtggccgacg acggcagtat cgaagtgcta accgcggccg aggaatacgc cgcggtcaaa
      481 caggcgatga ccgatgccgg tctcgagccc gaggaatccg acctgaccat gcgtgcctca
      541 accaataccg aggtcgatct cgagaccgcg ggcaagctgc tgcgcctggt cgatctgcta
      601 gaagcctcgg acgatgtgca ggaagtttat tacaatgccg agatccccga agaggcctac
      661 gaggccgagg gctaatgatc atcctgggca tcgaccctgg atcgcgcatc accggcttcg
      721 ggcttatcga taatcacgct aaccgcatgg aatatatcca tagcggcagc atcaaggtca
      781 gcggcgatag cctgccgcaa cgcctgggat acatattcgc cgaaatcgag acggtaatac
      841 ggcaataccg tccgcaacaa atgggtatcg aaaacgtgtt catggcacgt aacgccgatt
      901 cggcgctgaa gctcgggcag gcgcgtggcg cggcgatctg cgccggtcac caggccgggc
      961 tcgaaatctc cgagtacgcg gcacgcgaaa tcaagcaggc aatcgtcggc accggcgcgg
     1021 ccagcaagga ccaggtgcag cacatggtaa aacgcctgct cggtattcgt catgaactgc
     1081 aggccgacga ggccgatggt cttgcgattg ctatctgcca tgcacaattt tatgctacgc
     1141 gtcaaaagac cgggctcgat agcagtcaac tcaagcgcag gagaagccgc cgaagatgat
     1201 cagccgatta acaggcattc tggccgccaa gcgagcgccg cagatcctga tcgattgtca
     1261 tggcgttggt tacgaggccg atgtatccat gaccaccttt tacaagctac ccgaagtcgg
     1321 ccaacagatc tctatctgga cgcatttgct ggtcagggag gattcgcaca gcctgatcgg
     1381 cttcgacaac gagcaggagc gcaagctgtt ccgccagttg atccgcatca ccggcgtggg
     1441 tccgaaaatg gcgctgacca ttctctcggg catcgacgaa cagcagtttg cgctatgtat
     1501 cgccaacaac gatatcgcca tgctgacccg acttccgggt gtcggcaaga aaaccgccga
     1561 acgcctgatt atcgaaatgc gtgacaagat agacgtaacc gcgggcgacc tgccgacgca
     1621 acaggccgcg gccggcgggc attcgatcgt caacgaggca atagaagcgc tgcaggcgct
     1681 gggctacaag ccaatcgacg cggaaaagat gatcagccgt gcgcagcagc agggtgaatc
     1741 cgccgacagc gcctcgcagt tgatcaaaca ggcgttacag accacggtga agatatgagc
     1801 gaagaacgtc tgattagcgc cgaagcggtc gaagaggaat taatccagga ccgcgcactg
     1861 cggccgaaaa tgctggccga ttacgttggc caggaagaag tgcgcgagca gatggagatc
     1921 tttgtgcagg ccgcggttca gcgccgcgaa gcgctggatc acgtgctgat ctttgggccg
     1981 cccggtctcg gcaagaccac gctgtcgcat attattgcca atgaaatgca ggtcaacctg
     2041 cgtcaaacct cgggcccggt gctcgagaag gcaggcgatc tggcggcgat cctgaccaac
     2101 ctcgaggcgc atgacgtgct gttcatcgac gagattcatc gactgagccc ggtcgtcgaa
     2161 gaaattcttt acccggcgat ggaagacttt cagctcgata tcatgatcgg cgagggcccg
     2221 gcggcgcgtt cgatcaaact cgatctgccg ccctttaccc t
//
LOCUS       DATCZV010000631         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_104535, whole genome shotgun sequence.
ACCESSION   DATCZV010000631 DATCZV010000000
VERSION     DATCZV010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104535"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="hemC"
                     /locus_tag="VIW27_14115"
     CDS             <1..781
                     /gene="hemC"
                     /locus_tag="VIW27_14115"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5740860.1"
                     /translation="DAPLAKVGGKGLFVKELEAGLLDGRADLAVHSLKDVPAELPPGL
                     ELALVMEREDPRDAFVSGRYENLAAMPAGARVGTSSLRRQMQIRAAYPELDLDWLRGN
                     VNTRLAKLDAGEFDAIILAAAGLRRLGFDERIRTAIEPEECLPAIGQGVLGIEIRSDD
                     DELRELLAPLAHAETTLRVAAERAFNQTLAGSCQVPIAGYAVLDGDELYLRGLVGEPD
                     GSEILRAERRGSGARARELGEQLAGELLARGADKILARLRE"
     gene            784..1545
                     /locus_tag="VIW27_14120"
     CDS             784..1545
                     /locus_tag="VIW27_14120"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5740861.1"
                     /translation="MPPRLQGQVILNTRPAHQQAELTALLERDGARVLSFPVIEIVAS
                     GTPLAQPVDHDILLFVSRNAVDHGLDLLDATRLPPGVQLGVIGAATRAALLERVGDVG
                     ERLIESQPFNSEALLEADALQRVAGKRILILRGQAGRNLLGDQLSARGAQVDYLEVYR
                     RELPRHDPAAFERLTAERFPTLVVLTSNEGMHNLFELVETAAAARLRAIPWLLISERM
                     RESALKLGHNGPIVIAHGASDDGIWQTIREWAKQR"
     gene            1581..>2780
                     /locus_tag="VIW27_14125"
     CDS             1581..>2780
                     /locus_tag="VIW27_14125"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY5740862.1"
                     /translation="MSKQDSSSESGRNATEQADESGEPVARGDESGDREPLETTAEGE
                     ASATVVASSDEPASKDRREAPDTDSEPAPDSLTETDAEAASAGEIETASGDSAGTEQP
                     SGDSEPVGEALPPPKPPKRRMSWFGLFNFLLILAIVGAGGYYWWLEQQVLKDYDATIA
                     DLRAQLQTKAAGSRLDSGLSSVRGEIGGLERKLGELETGQQGLRESSEKLYELFGRDR
                     ADWQLAEVEYLMRVAQHKLILENDFAGAAITLQAASDRIGLTGDPGLLPVRITISEEI
                     AELKTRERPDLVGMTLTLARLARQARRLQPGFAPRIDESGEIQAPVQAPEDWRDRVLE
                     FIDSLVEVRHEAVAPTEIEANIVNVGETLEDNLKLARWAVLDRDARQYARLIDRSLQL
                     MSEFYDLD"
BASE COUNT          487 a          924 c          961 g          408 t
ORIGIN      
        1 cgacgcgccg ctcgccaagg tcggcggcaa ggggctgttc gtcaaggagc tcgaggccgg
       61 gctgctcgac gggcgcgccg atctcgcggt gcattcgctc aaggacgtgc cggcggagct
      121 gccgccgggg ctcgagctgg cgctggtcat ggagcgcgag gacccgcgcg atgcgttcgt
      181 ctccggccgc tacgaaaatc tcgccgcgat gccggccggc gcgcgcgtcg gtacctccag
      241 cctgcgccgc cagatgcaga tacgcgccgc ttaccccgaa ctcgacctcg actggctgcg
      301 cggcaatgtc aacacgcgcc tcgccaagct cgatgccggg gaattcgacg ccatcatcct
      361 cgccgcggcc ggcctcaggc ggctcggctt cgacgagcgc attcgcaccg cgatcgagcc
      421 cgaggagtgc ctgccggcga tcggtcaggg tgtgctcggc atcgagatcc gcagcgacga
      481 cgacgagctg cgggagctgc tcgcgccgct cgcgcatgcc gagaccacgc tgcgggtggc
      541 cgccgaacgg gccttcaacc agacgctcgc cggcagctgc caggtgccga tcgccggcta
      601 cgcggtgctc gacggcgacg agctctatct gcgcgggctg gtcggcgagc ccgatggcag
      661 cgaaatcctg cgcgccgagc gccgtggcag cggcgcccgg gcgcgcgagc tcggtgaaca
      721 gctggccggt gaattgctcg cgcgcggcgc ggacaagatt ctcgcacgac tgcgggagta
      781 gcgatgccgc cgcggctgca aggccaggtg atcctgaata cccggccagc gcatcagcag
      841 gcggagctga ccgcgctgct cgagcgcgac ggcgcgcggg tgctgtcctt tccggtcatc
      901 gagatcgtcg cctccggcac gccgctggcg caacccgtcg accacgatat cctgttgttc
      961 gtcagccgta acgcggtcga tcacggcctc gatctgctgg atgccacgcg gctgccgcca
     1021 ggtgtgcagc tcggtgtcat cggcgccgcc acccgcgccg cgttgctcga gcgcgtcggc
     1081 gatgtcggcg agcggctgat cgagagtcag ccattcaaca gcgaagccct gctcgaggcc
     1141 gatgcgctgc agcgggtggc cggcaagcgc atccttatcc tgcgcgggca ggccgggcgc
     1201 aacctgctcg gcgaccaact cagcgctcgc ggtgcgcagg tcgattacct cgaagtctac
     1261 cgccgcgaac tgccacggca cgatccagcg gcgtttgagc gactgaccgc ggaacgcttt
     1321 ccgaccctcg tggtgctgac cagcaacgag ggcatgcata acctgttcga actggtcgaa
     1381 accgccgccg ccgcgcgact gcgcgcgata ccgtggctgt tgatcagcga aagaatgcgt
     1441 gaatcggcgc tgaaactggg acataatggg cccattgtga tcgcgcacgg cgcgagcgac
     1501 gacggcatct ggcaaacgat tcgcgagtgg gcaaaacagc gctgaaatat tccggcctgc
     1561 gcggcacaac tgattaatct atgagtaagc aagattcatc cagcgaatcc gggaggaacg
     1621 ccacagagca ggccgacgaa agcggggagc ctgtcgcccg gggcgatgaa tccggtgacc
     1681 gcgagccgct cgagaccact gccgagggcg aggcgtccgc gaccgtcgtg gcaagcagcg
     1741 acgaacccgc gagcaaagac cgtcgcgaag cgccggacac ggacagcgag cccgcacccg
     1801 attcgctaac cgagaccgat gctgaagcgg cgtccgcggg cgaaatcgaa accgcctccg
     1861 gcgacagcgc cggcaccgag cagccttcgg gtgacagcga gcctgtcggc gaagccctgc
     1921 ctccaccgaa gccgccgaag cggcggatgt cgtggttcgg gctgttcaat ttcctgttga
     1981 ttctggcgat cgtcggcgcc ggcggctact actggtggct ggagcagcag gtgctgaagg
     2041 actacgatgc caccatcgcc gatcttcgcg ctcaactgca gaccaaggcg gccggctcgc
     2101 ggctcgacag cggcctgtcg tcggtcaggg gtgagattgg tggcctcgaa cgcaagctcg
     2161 gcgaactcga aaccgggcag caggggctgc gcgaatcgag cgaaaaactt tacgaattgt
     2221 tcggccgcga tcgcgccgac tggcaactcg ccgaggtcga atacctgatg cgcgtcgcgc
     2281 agcacaagct gattctcgag aacgactttg ccggcgccgc gattactctg caggcggcca
     2341 gtgaccgcat cgggctcacc ggtgatccgg ggctgctgcc ggtacggatc acgatcagcg
     2401 aggagattgc cgagctcaag acccgcgagc ggcccgacct ggtcggcatg acgctgacgc
     2461 tggcgcggct ggcgcgccag gcgcgcaggc tgcagccggg attcgcgccg cgcatcgacg
     2521 agagcgggga gatccaggcg cccgtgcagg cgccggagga ctggcgcgac cgcgtgctcg
     2581 agttcatcga ttcgctggtc gaggtgcgcc acgaggcggt ggcgccgacc gagatcgagg
     2641 ccaatattgt caatgtcggc gaaaccctgg aagacaatct caagctcgcg cgctgggcgg
     2701 tgctcgaccg cgacgcgcgc cagtacgccc gcttgatcga ccgcagcctg caactgatga
     2761 gcgagttcta cgatctcgac
//
LOCUS       DATCZV010000632         4506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_422504, whole genome shotgun sequence.
ACCESSION   DATCZV010000632 DATCZV010000000
VERSION     DATCZV010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422504"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /gene="nuoF"
                     /locus_tag="VIW27_14130"
     CDS             <1..1260
                     /gene="nuoF"
                     /locus_tag="VIW27_14130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5740863.1"
                     /translation="TTLDADQPWALDTYLAIDGYKAWKRILAEKTDPAAIIDNVKLSA
                     LRGRGGAGFPTGLKWSFMPRNAPGQKYIVCNSDESEPGTCKDRDILRFNPHALVEGMA
                     ICGYAIGATVGYNYMRGEFMDEPAQRFEAAVKEAYEAGYLGKNILGSGVDFDLYPVLG
                     AGAYICGEETALLESLEGKKGLPRYKPPFPANFGLYGKPTTINNTESLASTPSIIRNG
                     AQWFADLGIPNNGGVKCFSMSGHLNKPGNFEISMGTPFKELLQMAGGVRDGRKLKAVI
                     PGGSSVPVLPGEVMMECTMDYDSIAKAGSMLGSGAVIVMDETTDMVLALRRISRFYYS
                     ESCGQCTPCREGTGWLYRMLNRIIDGNGRPEDIQLLEDVSHKIEGRTICALGDAAAMP
                     VWSFIKHFRHEFEYYVEHKRSPVENAA"
     gene            1280..3643
                     /gene="nuoG"
                     /locus_tag="VIW27_14135"
     CDS             1280..3643
                     /gene="nuoG"
                     /locus_tag="VIW27_14135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY5740864.1"
                     /translation="MSDTITVTINGKTIETQAGRLLIDVADENDIVIPRFCYHKRLSV
                     AANCRMCLVDIERAPKPMPACATQCMDGMVVYTRSQKALAAQKSTMEFLLINHPLDCP
                     ICDQGGECELQDVAMGFGEGVSKYTESKRVVMDKNIGPLIATDLTRCIHCTRCVRFGE
                     EIAGMPELGATGRGEHMEIGTYIQKAIVSELSGNVIDVCPVGALTAKPSRYTARPWEL
                     TQHAVVSPHDCVGSNCFVHTCRDEVIRVVPRENEAINESWIADRDRFSYSALKAAQRL
                     TTPMLREQGVLKPVDWETALNEAAGRLGDAAADIAALISPQATLEEHYLAQKLLRALG
                     SDHIDHRLTQVDFSAQHQAPLMPWLGRSLESVDSLDAALIVGGSLRYEQPLLSHRLRK
                     AVLNNGAAVSSIGHLGGQYNFALQTELNGSPAQLVANLAGVARAIAAGGEPLGERLGK
                     LTGGVEPTPEQQAIASSLTAAGSAAIIVGLQALASPYLALIQELCEAITARCGATLGY
                     LSPSANSAGASLAGATPHRGPAGVPLDKPGENCAQILAAQHKVLLAIGFNPQLDACAA
                     KPLADNNEYVIAIASFDNDFVQQQADLVLPMAAIFETSGSYVNVEGLWQSFKGCVPAR
                     GDSRQGWKILAALGQVLAPGEFEYADTLAVRNELKDLCSEVSLSNLCGVESKLDSLPD
                     DAAKLQRLGITPIYAGDEMTRLSAPLQATPLMQMQTSVLMNARQAKLSKLADCDQVQV
                     RQDQATAVLPFRIDDSVPDGCVYVPAGIDAVCHLPAAFGKVELEKVS"
     gene            3673..>4506
                     /locus_tag="VIW27_14140"
     CDS             3673..>4506
                     /locus_tag="VIW27_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEY5740865.1"
                     /translation="MHEYVQYTIFSAAKALVILLPLFIVVAYYTLAERKVIGYMQVRK
                     GPNRVGPKGLFQPFADMFKLMFKEIIVPTNSSRYLFIIAPVITFAPAMAAWAVIPFDD
                     GLVFSDINAGLLYILAMTSIGVYGVIIAGWASNSKYALLGAMRSAAQIVAYEIAMGFA
                     LVGVLMVGGSLNLGEIVMRQQGGIGHWFFIPLLPLFFVYLISGVAETNRAPFDVAEGE
                     SEIVAGFHVEYSGMAFAVFFLAEYANMILIAMLCSLMFFGGWLSPVPGLVGDGIGWLF
                     GK"
BASE COUNT          836 a         1403 c         1443 g          824 t
ORIGIN      
        1 accaccctcg atgccgacca gccgtgggcg ctggacacct acctggccat cgacggctac
       61 aaggcctgga aaagaatact cgccgagaag accgacccgg ccgccatcat cgataacgtc
      121 aagctctcgg ccctgcgcgg ccgcggcggg gcgggctttc cgaccggcct gaagtggagt
      181 ttcatgccgc gcaacgcgcc cggccagaag tatatcgtct gcaactccga cgaatcggaa
      241 cccggtacct gcaaggatcg cgatatcctg cgcttcaacc cgcacgcgct ggtcgagggc
      301 atggccattt gcggttacgc gatcggcgcc accgtcggct acaactatat gcgcggcgaa
      361 ttcatggacg agccggcgca gcgtttcgaa gccgcagtga aagaagccta cgaggccggc
      421 tatctgggca agaatatcct cggcagcggg gtcgatttcg atctttaccc ggtgctcggt
      481 gccggcgctt atatctgcgg cgaggaaacc gcgctgctcg agtcgctgga aggcaagaaa
      541 gggctaccgc gctacaagcc gccgttcccg gccaacttcg gcctctacgg taaaccgacg
      601 acgatcaaca ataccgagtc gctggcctcg acgccgtcga tcatcaggaa cggcgcgcaa
      661 tggttcgccg atctgggcat ccccaacaac ggcggggtca agtgcttctc gatgtcgggc
      721 cacctcaaca agccgggcaa tttcgagatc tcgatgggca cgccgttcaa ggagctgctg
      781 caaatggctg gcggtgtgcg cgacgggcgc aagctgaaag ccgtgatccc gggaggttcc
      841 tcggtgccgg tgctgcccgg cgaagtgatg atggaatgca cgatggatta cgattcgatc
      901 gccaaagccg gctcgatgct cggctcgggc gcggtcatcg tgatggacga aaccaccgac
      961 atggtgctcg cgctgaggcg aatttcgcga ttttactact ccgagtcctg cggtcagtgc
     1021 acgccgtgcc gtgagggtac cgggtggctg taccgcatgc tcaatcggat catcgacggc
     1081 aatggccgcc ccgaggatat ccagttgctg gaagacgttt cgcacaagat cgaggggcgc
     1141 acgatctgcg cgcttggcga tgccgcggca atgccggtgt ggagcttcat caagcatttc
     1201 cgccacgagt tcgaatacta cgtcgagcac aagcgctcgc cggtcgaaaa cgccgcctga
     1261 gccgatagcg aaagattcaa tgagcgatac gataacagtc acgatcaacg gcaagaccat
     1321 cgaaactcag gccgggcgcc tgctgatcga tgtcgccgac gagaacgata tcgtgattcc
     1381 gcgtttctgc taccacaagc gcctgtcggt ggcggccaac tgccgcatgt gcctggtcga
     1441 tatcgagcgc gcgccgaagc cgatgccggc ctgcgccacg cagtgcatgg acggcatggt
     1501 ggtgtacacg cgatcgcaaa aggcgctcgc ggcgcaaaag tcgaccatgg aattcctgtt
     1561 gatcaaccac ccgctggatt gcccgatctg cgaccagggt ggcgaatgcg aactgcagga
     1621 tgtggcgatg ggtttcggcg agggcgtctc caagtatacc gagtccaagc gcgtggtcat
     1681 ggacaagaat atcggcccgc tgattgccac cgacctgacg cgctgcatac actgtacgcg
     1741 ttgcgtgcgc tttggcgagg aaatcgccgg catgccggaa ctcggcgcca ccgggcgcgg
     1801 cgagcacatg gaaatcggca cctatattca aaaggcgatc gtctccgagc tgtccggcaa
     1861 cgtcatcgac gtctgcccgg tcggcgcgct gaccgccaag ccgtcgcgct acaccgcgcg
     1921 gccctgggaa ctgacccagc acgcggtggt ctcgccgcac gattgcgtcg gctcgaactg
     1981 cttcgtgcat acttgccgcg acgaggtgat ccgcgtggtg ccgcgcgaaa acgaggcgat
     2041 caacgaatcc tggatcgccg accgggatcg cttcagttac agcgcgttga aagccgcgca
     2101 gcgcctgacg accccgatgc tgcgcgagca gggcgtgctc aagccggtcg actgggaaac
     2161 cgcgctcaac gaagccgccg gccgtctcgg cgacgccgct gccgacatcg ctgcgctgat
     2221 cagcccgcag gcgacgctgg aggagcatta cctggcgcag aagctgctgc gcgcactggg
     2281 ttccgaccat atcgatcacc ggttgacaca agtcgatttt tcggcgcagc accaggcccc
     2341 gctgatgccg tggctggggc gttcgctgga atccgtcgac agcctcgacg cggcgctgat
     2401 cgtcggcggc agcctgcgct acgagcagcc gttgctgtcg catcgattgc gcaaggcggt
     2461 attgaacaat ggcgcggcgg tatcgtcgat cgggcacctc ggcgggcaat acaatttcgc
     2521 gctgcaaacc gaactcaacg gctcgccggc gcaactggtc gccaacctcg ccggcgtcgc
     2581 ccgcgccatc gccgcgggtg gcgagccgct cggcgaacgg ctcggcaagc ttaccggcgg
     2641 agtcgagccg acgcccgagc agcaggcgat cgcgagttcg ctgaccgccg ccggcagtgc
     2701 cgcgatcatc gtcggactgc aagccctggc aagcccctac ctggcgctga tccaggaatt
     2761 gtgcgaggcg ataaccgcgc gctgcggcgc gacgctcggt tacctgtcgc cgtcggccaa
     2821 cagtgccggc gccagcctgg ccggcgccac tccgcatcgc ggtccggccg gcgtgccgct
     2881 cgacaagccc ggtgagaact gcgcgcaaat actcgccgcg cagcacaagg tgctgctcgc
     2941 gatcggtttc aacccgcagc tcgatgcctg cgccgccaag ccgctggctg acaacaacga
     3001 atacgtgatc gcgatagcca gtttcgacaa cgatttcgtg cagcagcagg ccgacctggt
     3061 gctgccgatg gcggcgatct tcgaaacctc cggcagctac gtcaacgtcg aggggctgtg
     3121 gcagagtttc aagggctgcg tgccggcacg cggcgacagc cgtcagggct ggaagattct
     3181 tgccgcgctc ggccaggtgc tggcgcccgg agaattcgag tacgcggata cgctggcggt
     3241 cagaaacgaa ctcaaggacc tgtgcagcga ggtcagtctc agcaacctgt gcggtgtcga
     3301 gtcgaaactc gacagtctgc cggatgatgc cgctaaattg cagagactcg gcatcacgcc
     3361 gatctatgcc ggcgacgaga tgacccggct gtcggcgccg ctacaggcga cgccactgat
     3421 gcaaatgcaa acctcggtgc tgatgaacgc ccggcaggcg aagctgtcga agctggcgga
     3481 ttgtgatcag gtgcaggtca ggcaggatca ggcgaccgcg gtgctaccgt ttcgcatcga
     3541 tgactcggtg cctgacggct gcgtttatgt gccggccggt atcgatgccg tgtgccatct
     3601 gccggcagcc tttggcaagg tcgaactgga gaaggtgtcg tagtggagtt gctgatctcc
     3661 atctgggaat ggctgcacga atacgtgcag tatacgattt tcagcgccgc caaggcgttg
     3721 gtcatcctgt tgccactgtt catcgtcgtc gcctattaca cccttgccga gcgcaaggtc
     3781 atcggctaca tgcaggtccg caagggcccg aaccgggtcg ggccgaaggg cctgttccag
     3841 ccgttcgcgg acatgttcaa gctgatgttc aaggaaatta tcgttccgac caattccagc
     3901 cgctacctgt tcatcatcgc gccggtgatc acctttgcgc cggcgatggc cgcctgggcg
     3961 gtgattccgt tcgacgacgg cctggtgttc agcgacatca atgccggcct gctgtatatc
     4021 ctggcgatga cctcgattgg cgtctacggc gtcatcatcg ccggctgggc gtcgaactcg
     4081 aagtacgcac tgctgggcgc gatgcgctcg gcggcgcaga tcgtggccta cgaaatcgcc
     4141 atggggttcg cgctggttgg tgtgctgatg gtcggcggca gcctcaacct cggcgaaatc
     4201 gtcatgcgcc agcagggggg catcggccac tggtttttca ttccgctgct gccgctgttt
     4261 ttcgtttacc tgatttccgg ggtcgccgaa accaaccgcg cgccgttcga cgtcgccgag
     4321 ggcgaatcgg agatcgtcgc cggtttccat gtcgagtact cgggcatggc cttcgcggtg
     4381 ttctttctcg ccgaatacgc caacatgatc ctgatcgcga tgctctgttc gctgatgttt
     4441 ttcggcggct ggttatcccc ggtaccgggc ctggtgggcg acggtatcgg ctggctgttc
     4501 ggcaag
//
LOCUS       DATCZV010000633         4363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_104657, whole genome shotgun sequence.
ACCESSION   DATCZV010000633 DATCZV010000000
VERSION     DATCZV010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104657"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VIW27_14145"
     CDS             complement(<1..1205)
                     /locus_tag="VIW27_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY5740866.1"
                     /translation="MAEKQTGSGFIGWIDARFPLTKMFEEHLSKYYAPKNFNFWYFFG
                     SLALLVLVIQIITGIFLTMHYKPDGDMAFASVEYIMRDVDWGWLIRYLHSTGASAFFI
                     VVYLHMFRALIYGSYQKPRELIWLFGMFIFLALMAEAFMGYLLPWGQMSYWGAQVIIS
                     LFGAIPWVGDELTLWIRGDFVVSDATLNRFFALHVVAVPLVLLMLVVMHIIALHEVGS
                     NNPDGVEIKKNKDANGIPLDGIPFHPYYTVKDIVGVVVFLILFSVVVFFTPEMGGYFL
                     EYANFVPANQFKTPEHIAPVWYFTPFYAILRAIPDKLLGVIAMFAAIIVLFLLPWIDR
                     GRVKSVRYRSSAFKWLLVMLVVSFIGLGYLGARPATETRTIFARLFSLGYFGFFLGLW
                     WISKNEVTKP"
     gene            complement(1205..1780)
                     /gene="petA"
                     /locus_tag="VIW27_14150"
     CDS             complement(1205..1780)
                     /gene="petA"
                     /locus_tag="VIW27_14150"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY5740867.1"
                     /translation="MSSDEVDSNRRRFLTAGTVVVGGVGTAIAAWPFLRSWSPSARAK
                     TAGAPVEADISKLQPGQLIIVKWRGKPVWVVRRDQKALDTLAKIEDELRDPDSQQSIQ
                     PEYANNQFRSRKPEFLIMEGICTHLGCSPTYRPEITTDFQGGFFCPCHGSKFDFAGRV
                     YQGVPAPTNLTVPPHYFVSDNVVLIGEDGVA"
     gene            complement(1995..2741)
                     /locus_tag="VIW27_14155"
     CDS             complement(1995..2741)
                     /locus_tag="VIW27_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY5740868.1"
                     /translation="MRLQELCEFCDQYLDAEQFSDYCPNGLQVEANPEVEHLVCGVTA
                     SQALIDAAIELGADAILVHHGYFWKGEAQPITGIKGRRVAGLIRNNINLLAYHLPLDA
                     HPEVGNNAQLARVMNWQVGERFAEQQLVFEGNLAQPQSLDELSRDIETRLDTRALAIA
                     AGEHRIERLAWCTGAAQGFIEQAAARGVDAFVSGEVSEQTVHLARELGIHYIAAGHHA
                     TERYGVQALAAEIAARFGIRQQFVDIANPV"
     gene            2785..3942
                     /locus_tag="VIW27_14160"
     CDS             2785..3942
                     /locus_tag="VIW27_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5740869.1"
                     /translation="MKLIKAAGFLLQFGLIGLALAVIYLYASSDRSRDEILSFLGSSA
                     PPAVQLPAGGVPVAYSYADAVASSTSSVVTIYTSKTVEEKPHPLLDDPVFSRFFGDQL
                     ERRRRNRSETNLGSGVIIGSEGFILTNQHVIDGADEILISLADGRGSQALLIGEDRES
                     DIAILQVPLGGLSGIRIADSASLRVGDVVLAIGNPLNVGQTVTMGIVSATGRNRIGLN
                     TFENFIQTDAAINPGNSGGALVNARGELIGINTAIFSQAAGAQGIGFAIPISLALDIM
                     QQIIEHGEVTRGWLGVEGTEISTNAAQATGNPGIRGALIVGVFIDSPADMAGIRAGDI
                     VVAVDGSPVHGIRDLLDQITRKRPGERVEVELYRGTERLELQMQVSQRPHE"
     gene            complement(4175..>4363)
                     /locus_tag="VIW27_14165"
     CDS             complement(4175..>4363)
                     /locus_tag="VIW27_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740870.1"
                     /translation="TQFITVLGVTYDIIAIGTEATVFEPLGLEGSIVPGTTFVEVEDE
                     AVGGDNPPGTAEKIGLED"
BASE COUNT          904 a         1348 c         1330 g          781 t
ORIGIN      
        1 ggcttggtca cctcgttttt actaatccac cacaggccaa ggaaaaagcc gaaatagccc
       61 aggctgaaca ggcgcgcgaa gatggtgcgc gtctcggtgg caggccgcgc gccgagataa
      121 ccgaggccga tgaacgagac caccagcatg accagcaacc acttgaaggc cgaggagcgg
      181 tagcgcaccg acttgacccg gccgcggtcg atccacggca acaggaacaa aacgatgatc
      241 gccgcgaaca tcgcgatgac accgagcagc ttgtcgggaa tggcgcgcag aatcgcgtag
      301 aacggcgtga aataccagac cggcgcgatg tgctccgggg tcttgaactg gttggcgggt
      361 acgaagttcg cgtactcgag gaagtagccg cccatttccg gggtgaagaa aaccaccacg
      421 ctgaacagga tcaggaagac gacgacaccg acgatatcct tgaccgtgta atacgggtgg
      481 aacggaatgc catccagcgg aatgccgttg gcgtccttgt ttttcttgat ttcgacaccg
      541 tccgggttgt tcgagccgac ctcgtgcagc gcgatgatat gcattaccac cagcatcagc
      601 agcaccagcg gcacggccac cacgtgcagc gcgaagaagc ggttcagggt cgcgtcggag
      661 accacgaaat caccgcggat ccacagcgtc agctcgtcgc cgacccacgg aatggcgccg
      721 aacagcgaga tgatgacctg cgcgccccag taagacatct gcccccaggg cagcaggtaa
      781 cccataaacg cctcggccat cagggcgaga aaaatgaaca tgccgaacag ccatatcagc
      841 tcgcgcggct tctggtagga gccatagatc agcgcgcgga acatgtgcag gtagacgacg
      901 atgaaaaacg ccgacgcgcc ggtcgaatgc aggtagcgga tcagccagcc ccagtcgaca
      961 tcgcgcatga tgtactcgac cgaggcgaaa gccatgtcgc cgtccggctt gtaatgcatg
     1021 gtcaggaaaa ttccggtgat gatctggatc accagcacca gcagtgccag cgaaccgaaa
     1081 aagtaccaga aattgaaatt cttcggcgcg taatacttcg acagatgttc ctcgaacatc
     1141 ttggtcagcg ggaaacgcgc gtcgatccag ccgatgaagc cggagccagt ctgtttttca
     1201 gccattatgc taccccgtcc tcgccgatca gcaccacgtt gtcactcacg aaataatgcg
     1261 gcggcacggt caggttggtc ggtgccggca cgccctggta aacccggcca gcgaaatcga
     1321 acttggaacc atgacaggga cagaagaaac caccctggaa atcagtggtg atttccggcc
     1381 ggtaagtggg cgagcaaccg agatgggtgc aaatgccttc catgatcaga aactccggct
     1441 tgcgcgagcg gaactggttg ttggcgtatt ccggctggat gctctgctgc gaatcgggat
     1501 cgcgcaactc gtcctcgatc ttcgccagcg tatcgagggc cttctggtcg cggcgcacga
     1561 cccataccgg tttgccgcgc cacttgacga tgatcagctg gcccggttgc agcttgctga
     1621 tgtcggcctc gaccggcgcg ccggcggtct tcgcccgcgc gctggggctc cagctcctga
     1681 gaaaaggcca ggcggcaatg gccgtgccaa cgccgccaac gaccacggtg cccgcagtca
     1741 gaaaacgcct gcggttgcta tctacttcgt ctgaggacat acgacttgta atcctggtaa
     1801 agaatcgtca aatgggtatc gagtatccac gaaaaacagc ctcgcgctgc agtgcccgcc
     1861 tgcgacccgc aaggcctgaa tcccggacat aaaaaaacgg ccgctagcat agcttacttt
     1921 gtgattttcg tcgagggcat tcggcgcgat taccgcgtgc gacgacggat ttttagcgaa
     1981 tgttacggca ttaattaaac cggattggcg atatcgacga actgctggcg aatgccgaag
     2041 cgagcggcga tctccgcggc cagtgcctgc accccgtaac gctcggtcgc gtggtggccg
     2101 gcagcgatgt aatgaatgcc cagctcgcgg gccaggtgca cggtctgctc cgagacttca
     2161 ccgctgacga acgcatcgac cccgcgcgcc gccgcttgct cgataaatcc ctgcgcggcg
     2221 cccgtgcacc aggcgagccg ctcgatgcga tgctcgccgg cggcgatcgc cagcgcgcgc
     2281 gtgtcgagcc gggtttctat atcgcggctg agttcgtcga gcgactgcgg ttgcgcgaga
     2341 ttaccttcga ataccagttg ctgctcagcg aaacgttcgc cgacctgcca gttcatgact
     2401 cttgccagct gcgcgttgtt gccgacctcg gggtgggcgt cgagcggcag gtgataggcc
     2461 agcaagttga tgttattgcg tatcaggccg gccacgcgcc ggcccttgat gccggtaatc
     2521 ggctgcgcct cgcctttcca gaaatagccg tgatgcacga ggatggcatc ggcgccgagt
     2581 tcgatggcgg cgtcgatcag tgcctggctg gcggtgaccc cgcaaaccag gtgttcgacc
     2641 tcggggttcg cctcgacctg caaaccattc ggacagtagt ccgaaaactg ctcggcatcg
     2701 aggtactgat cgcagaattc gcagagttcc tgtaaacgca ttcgacttga ttccgggaaa
     2761 cagacgctat gatactatcg gtcgatgaaa ctaatcaaag ccgccggctt cctgttgcag
     2821 ttcgggctca tcggcctggc gcttgcggtc atctacctgt atgcgtcgag cgacaggagc
     2881 cgcgacgaga tcctgtcgtt tctcggctct tcggcgccgc ctgcagtaca gctgcctgcg
     2941 ggtggcgtcc cggtggcgta ttcctatgcc gatgccgtcg cctcgtcgac atcatcggtg
     3001 gtaacgatct acacctcgaa gacggtcgaa gaaaaaccac acccgctgct cgacgatccg
     3061 gtattcagcc gcttcttcgg cgaccagctc gagcggcgcc gtcgcaaccg cagcgaaacc
     3121 aatctcggtt ccggggtgat catcggctcc gagggattca tcctgaccaa ccagcacgtc
     3181 atcgatggcg ccgacgaaat cctgatttca ctggccgacg ggcgcggcag ccaggcgttg
     3241 ctgatcggcg aggaccggga atccgatatc gccatcctgc aggtaccgct gggcggcctc
     3301 agcggcatcc ggatcgcgga ctccgcgagc ctgcgcgtcg gcgacgtggt gctggcgatc
     3361 ggcaatccgc tgaacgtcgg ccagacggtg accatgggca tcgtcagcgc caccggccgc
     3421 aatcgcatcg gtctcaacac ctttgagaac ttcatccaga ccgatgccgc gatcaatccc
     3481 ggtaattccg gcggcgcgct ggtcaacgcg cgcggcgaac tgatcggcat caacacggcg
     3541 attttcagcc aggcggcggg cgcccagggc atcggcttcg cgatcccgat ctcgctggcg
     3601 ctcgatatca tgcagcagat catcgaacac ggcgaggtga cgcgcggctg gctcggcgtc
     3661 gagggcaccg agatcagcac caacgcggca caggccaccg gcaatccggg aattcgcggc
     3721 gcgttgatcg tcggtgtgtt tatcgacagc cccgcggaca tggccgggat tcgcgccggc
     3781 gacatcgtgg tcgccgtcga cggcagcccg gtgcacggta ttcgcgacct gctcgaccag
     3841 atcacccgca agcgtcccgg ggagcgggtc gaggtcgagc tctaccgcgg caccgagcga
     3901 ctggagctgc agatgcaggt ctcgcagcgg ccgcacgaat agccggggcg gccgcatcgt
     3961 catcgtggtc gacgttcggc cagttcggcg tattcgtgat ccgctgcacg gcgatccgag
     4021 tcctgcccgg ggaaaccttc gcgatcattc tgtaccggga ccccgaacgg ggaaaaattg
     4081 agatgcgggt taccgagccg ccgcaataat tccacgctcg ccagccccgc accacggcgg
     4141 gtgcgcggcc ggcccgtgaa cggattagcc gggattagtc ttccagcccg atcttctcgg
     4201 cggtgccggg aggattatca ccgccgaccg cttcgtcctc gacttcgacg aaggtcgtcc
     4261 ccggaactat agacccctcc aatcccaacg gctcgaaaac ggtggcttcc gtaccgatag
     4321 cgataatatc gtaggtgaca ccgagcaccg tgataaattg agt
//
LOCUS       DATCZV010000634         6121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_422975, whole genome shotgun sequence.
ACCESSION   DATCZV010000634 DATCZV010000000
VERSION     DATCZV010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422975"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VIW27_14170"
     CDS             complement(<1..303)
                     /locus_tag="VIW27_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5740871.1"
                     /translation="MKASELFVRCLENEGVEYVFGVPGEENLDVMDALIGSPIRFVTT
                     RHEQGAAFIADVYGRLTGKAGVCPSTLGPGATNLITGFADANMDRAPIVGIAGQGAT"
     gene            549..1367
                     /locus_tag="VIW27_14175"
     CDS             549..1367
                     /locus_tag="VIW27_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5740872.1"
                     /translation="MENVTTWISEHALDWSIQIGIAIAIFVIGKIAARMISNLIRRGL
                     RRGGTDDMLVGFIGNISYAVLLVAVVLAAVDSLGVNVTSLMAILGAAGLAVGLALKDS
                     LGNFAAGVMIIIFRPFKIGDYVEAGGTAGTIDEIGLFATLMHTPDNKRIIVPNGSIIN
                     GNIINVSALPTRRVDLVFGISYDDNIGQARDIIVQILGEDERILAEPAPTVTVGALAD
                     SSVNLNVRPWVNAGDYWGVYWDLLEKVKVAFDEAGISIPYPQQDVYMHEVKAAS"
     gene            complement(1612..2481)
                     /locus_tag="VIW27_14180"
     CDS             complement(1612..2481)
                     /locus_tag="VIW27_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5740873.1"
                     /translation="MTGNYQFLEVHPSSPALGAEIGGVDIAAGVDDAQFAELRRAFAE
                     YGVIFLRDQQITPQQHIEFARRWGEINVNRFFRAVDDYPLIAEVRKEAHQKANIGSSW
                     HTDHSYDQIPAMGSLLYAREVPEVGGDTLFASMYAAYDALSDGFKALLSTLRALHSSR
                     HAFGEGAYIGADLDDVGGRLGNTEAATQDAIHPVIIRHPLSGRPALYVNGDFTLRFDG
                     WSEAESRPLLDYLYAHASRAEFTCRFRWRAGSMAIWDNRATHHCALNDYQGLRRLMHR
                     ITIEGEPLAAAAA"
     gene            complement(2539..3738)
                     /locus_tag="VIW27_14185"
     CDS             complement(2539..3738)
                     /locus_tag="VIW27_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5740874.1"
                     /translation="MQSDANFFVDEEAERRIQSHRLQRVRERLRAADCAAIVLFDPIS
                     LRYATGTRNMQVWSMHNLFRYAVVFESGETVLFELPSSAHLARGRVDDIRPSLSTDYL
                     AVGGRGEEMGRRWAESIAGLLREKGVAGERLAIDRADLALVLAAQRESLRLVPGQPIM
                     EIARAIKSADEIGALRLSLQSCEHSVAAMRERLQPGMRESDALAMLVDGSIRRGGEYP
                     ETRLLTSGPRTNPWFQETSDRVLENGDMLSFDTDLIGPMGFYNDISRSWVVGDRRPDD
                     EQRRLYALAYAQLQHNTALLQPGLGFLEYSDKAFPLPDNCLPNRYADVAHGCGLGVEY
                     PLIWYREDEQWGAYDGVFEEDMVVCVECYVGEVDGREGVKLEQPVWLTADGPVVLAEF
                     PFELDYL"
     gene            3871..4254
                     /locus_tag="VIW27_14190"
     CDS             3871..4254
                     /locus_tag="VIW27_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HEY5740875.1"
                     /translation="MNLERVLFGFVTILALAFTWAFVMGNIESASHHNVWMLTIAILI
                     NLIATGLKLGDRSQVGALLLAGSLVVDVLLITARVVWVIAENDTPTGPGAESIANIVS
                     LAGGALVASIVTVVILVGDTLISRR"
     gene            4536..>6121
                     /locus_tag="VIW27_14195"
     CDS             4536..>6121
                     /locus_tag="VIW27_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY5740876.1"
                     /translation="MATTSELDRVSMIVLRYMRGPIFVLIVVYAIGITGMALIPGQDA
                     DGNPAHMSLFHAFYFFTYTATTTGFGEIPNGFTDAQRLWAIFCIYIGVVAWLYAIGSI
                     FRLVQNPHFLLALNEHRFARSVRAIRQPFYIVCGFGDTGSLLARGLSDHWLRGVIIDI
                     DPERIKALRLRDYNVHMPGLCADPTVPKHLIDAGIRMADCRALVILTVNEEINTRVAT
                     MARLLNPELHILCRSSSRRHIEQLKSLGNVTIINPFEIFAQLLSMAITAPRLHNLNSC
                     LVRNPMARLGEPIEVPTGDWIVCGYGRMGRWMCRHFVDNGIKPVIIDPEAKHLEGAAR
                     IINDHANRESLQAAGIDHAAGVVAGTDSDHVNLNILMSVKALKPDAFTIVRQNSHENQ
                     IAFDAARADLVLQSSLTTARRVLKHLISPQVQHFVEYLREQGEDICQQAVERLKSMIG
                     DRPLHLWQVTLRSSKAEAVIEHLAAKRSLTLGELRRDPRDLDASLPCMPLSIERGERR
                     IMLPADDEPLRVGDKLLFCGT"
BASE COUNT         1114 a         2005 c         1927 g         1075 t
ORIGIN      
        1 ggtcgcgccc tgcccggcga tgccgacgat cggcgcgcgg tccatgttgg catcggcgaa
       61 gccggtgatc aggttggtgg cgccgggacc gagcgtcgac gggcagaccc cggccttgcc
      121 ggtcagccgg ccgtagacgt cggcgatgaa cgccgcgccc tgctcgtggc gggtagtgac
      181 aaagcggatc gggctgccga tcagcgcgtc catgacgtcg aggttctctt cgccgggtac
      241 gccgaacaca tactcgacgc cctcgttttc gaggcagcgg acaaacaatt cgctggcttt
      301 catgcgagcc tccctggacc ctggcgcaca gcgaggtgcg cggtaaagta acggccgggt
      361 gcggcagcaa cggcaatgcc ggcggcgata cgcggcgacc cggaatgatg tttcgaccct
      421 aacataattg atccgggcct gctgcatcga gtcagccatg gcggggaaaa attactcccc
      481 aaagccgccg gcaaatggga caatcgcgcc gactgaattc aagacaccga aacaccgaag
      541 gagaaatcat ggaaaacgtc actacctgga tctcggagca cgcgctcgac tggagcatcc
      601 agatcggtat cgccatcgcg atcttcgtca tcggcaagat cgccgcacgc atgatcagca
      661 acctgatccg cagggggctg cgccgcggcg gcaccgacga catgctggtc ggcttcatcg
      721 gcaacatcag ctacgcggtg ctgctggtcg ccgtggtgct ggcggcggtc gattcgctgg
      781 gcgtcaacgt cacctcgctg atggccatcc tcggtgccgc cggactcgcg gtcggcctgg
      841 cgctcaagga ctcactcggc aacttcgccg ccggcgtgat gatcatcatt ttccggccgt
      901 tcaagattgg cgactatgtc gaagccggcg gcacggctgg cacgatcgat gaaatcggcc
      961 tgttcgcgac actgatgcat acccccgaca acaagcgcat catcgtgccc aacggctcga
     1021 tcatcaacgg taatatcatc aatgtcagcg ccctgccgac gcggcgcgtg gacctggtgt
     1081 tcggcatcag ctacgacgac aacatcggcc aggcgcgcga tatcatcgtg cagatcctcg
     1141 gcgaggacga gcgcatactg gccgagccgg cgccgaccgt cactgtcggc gcgctggccg
     1201 acagcagcgt caacctgaac gtccgcccgt gggtcaatgc cggcgattac tggggggttt
     1261 actgggacct gctggaaaag gtcaaagtcg ccttcgacga ggcagggatc tcgatcccgt
     1321 acccgcagca ggacgtctac atgcacgagg tcaaggcagc cagctagcag ctcgctgcag
     1381 tgccgcccgg gcggcacgat atcgccggga aacggcaatt gatcggctgg taaacgctgc
     1441 cagggttgcg ggtaaaccgc gctgcggccg agggaatctg cgactgcccg ctggccccgg
     1501 gggcgccgtc cccacgcctg gcagagcggc ctggtccgat gttgccgcgt accgcaagcg
     1561 cccgggttaa ttgatcgagc tgtccgtcag tccggtcgcc gccaggatga atcaggcggc
     1621 cgctgccgcg agcggttcgc cctcgatggt gatgcggtgc atcaggcggc gcaggccctg
     1681 gtagtcgttg agcgcacagt gatgtgtggc gcgattgtcc cagatagcca tcgagcctgc
     1741 gcgccagcga aagcggcagg tgaattcggc gcggctggca tgcgcataga gatagtcgag
     1801 cagcggccgc gattcggcct cgctccagcc gtcgaagcgc agcgtgaaat cgccgttgac
     1861 gtagagcgcg ggccggcccg acagcgggtg acgaatgatc accgggtgga tcgcgtcctg
     1921 ggtcgcggcc tcggtattgc cgagccgtcc gcccacatcg tcgagatcgg cgccgatgta
     1981 ggcgccctcg ccgaacgcgt ggcggctcga atgcagcgct cgcagcgtcg acagcagcgc
     2041 cttgaaaccg tccgaaagcg catcgtaggc ggcgtacatg ctggcgaaca gcgtatcgcc
     2101 gccgacctcc ggcacctcgc gcgcgtacag cagcgagccc atcgccggga tctgatcgta
     2161 ggagtgatcg gtgtgccagc tggagccgat gttggctttt tggtgggctt ccttgcgcac
     2221 ctcggcgatc aacgggtagt cgtcgaccgc gcgaaagaag cgattgacgt tgatctcgcc
     2281 ccagcgacgc gcaaactcga tgtgctgctg cggcgtgatt tgctggtcgc gcaggaagat
     2341 cacgccgtac tcggcaaacg cacgtcgcaa ctcggcgaac tgtgcatcgt cgacaccggc
     2401 ggcgatatcg acaccgccga tctcggcgcc gagcgccggg ctcgacgggt gcacctccag
     2461 gaattggtaa ttgccggtca tcggcgggat actacgcggc gcggcggagc gaatcaatca
     2521 atcgcccggc gccaatgctt acaggtaatc cagttcgaac gggaattccg ccagcaccac
     2581 cgggccgtcg gcggtgagcc acaccggctg ctcgagcttg acgccctcgc gcccgtcgac
     2641 ctcgccgacg tagcattcca cgcaaaccac catgtcctct tcgaacacgc catcgtaagc
     2701 gccccactgc tcgtcttcgc gataccagat cagcgggtac tcgacaccga gcccgcagcc
     2761 gtgggcgacg tcggcgtagc gattcggcag gcagttgtcg ggcagcggaa aagccttgtc
     2821 ggaatactcg agaaaaccga gaccgggctg caacagcgcg gtattgtgct gcaattgcgc
     2881 gtaagcgagc gcatacagcc ggcgttgctc gtcgtcgggc cggcgatcgc cgaccaccca
     2941 gctacgcgaa atgtcgttgt agaaacccat cggcccgatc aggtcggtat cgaaggacag
     3001 catatcgccg ttttcgagca cccgatccga ggtttcctgg aaccacgggt tggtgcgcgg
     3061 gccgctggtc agcaggcggg tctccgggta ttcgccgccg cgccgaatgc tgccgtcgac
     3121 cagcatcgcc agtgcgtccg actcgcgcat tccgggttgc aggcgctcgc gcatcgccgc
     3181 gacgctgtgt tcgcagcttt gcagcgacag ccgcaacgcg ccgatctcgt ctgcggactt
     3241 gatcgctcgc gcgatttcca tgatcggctg gccgggcacc agtcgcagcg actcgcgctg
     3301 cgcggcgagc accagcgcca ggtcggcgcg gtcgatcgcg aggcgttcgc cggccacgcc
     3361 cttttcgcgc agcaagccgg cgatcgactc cgcccagcgc cggcccatct cctcgccgcg
     3421 cccgccgacc gccaggtaat cggtactcaa cgacggccga atatcgtcga cccggccgcg
     3481 cgccaggtga gccgagctcg gtaactcgaa cagcacggtt tcaccgctct cgaagaccac
     3541 cgcgtagcga aacaggttgt gcatgctcca cacctgcatg ttgcgcgtac cggtcgcgta
     3601 gcgcaggctg atcgggtcga acagcacgat ggcggcgcaa tcggccgccc gcaggcgctc
     3661 gcgcacgcgc tgcagccgat ggctctgaat gcggcgctcg gcttcctcgt cgacgaaaaa
     3721 attcgcatcg ctttgcattc gctgccggtg ccgaagcgga aagtcatcga aatcatacag
     3781 gcatcggtgg cgaatgcgac agtttcggca cgggtgacga agccgccaca tcggcgtatt
     3841 cttgtcgcta aaacctaacg gagcgaggac atgaacctgg aacgggtgct tttcggattc
     3901 gtcacgattc tggcgctggc cttcacctgg gccttcgtga tgggcaatat cgagtccgcc
     3961 agccatcata acgtctggat gctgaccatc gccattttga tcaacctgat cgccaccggc
     4021 ctcaaactcg gcgatcgctc ccaggtcggt gcgctgctgc tggcgggcag cctggtggtc
     4081 gacgtgctgc tgattaccgc gcgcgtggtc tgggtgatcg ccgagaacga tacgccgacg
     4141 ggccccggcg cggaatcgat cgccaatatc gtatcgctgg ccggcggtgc gctggtcgcc
     4201 agcatcgtca ccgtcgtgat cctggtcggc gatacgctga tttcgcgccg ttagtcagcc
     4261 cgagtaatgc agccgccacg atctagcctc gcatcagccg aataacttgc ccgagaaaca
     4321 gagtgatttc gcagtttgag acaacagacc cattggtgat cggctcgctc tccattccgg
     4381 cgccgcgtgt tttacgtgga tctggatcga gtcttattca gagtcatggc tctggctacg
     4441 ggtctcgctg cggagcggcg cccggattca cctgccccgc cccggcaagc gggatgctgt
     4501 cagccggcaa accgctgcgg tacccgggct aggcgatggc cactacctcg gaactcgacc
     4561 gcgtctcgat gatcgtgctg cgctacatgc gcggcccgat attcgtgctg atcgtcgtct
     4621 acgcgatcgg tatcaccggc atggcgctga tccccggaca ggacgccgac ggcaaccccg
     4681 cgcacatgag cctgttccac gcgttttact ttttcaccta taccgcgacc accaccggct
     4741 tcggcgaaat cccgaacggg tttaccgacg cccagcgcct gtgggcgatc ttctgcatct
     4801 acatcggggt agtcgcctgg ctgtacgcga tcggctcgat cttccgcctg gtgcagaacc
     4861 cgcatttcct gctggcgctc aacgagcacc gtttcgcgcg ctcggtacgc gcgattcgtc
     4921 agccttttta catcgtctgc ggctttggcg acactggcag cctgctggcg cggggcctca
     4981 gcgaccactg gctgcgcggc gtgatcatcg atatcgatcc ggaacgcatc aaggcgctga
     5041 ggctgcgtga ttacaacgta cacatgccag gcctgtgcgc cgaccccacg gtgcccaagc
     5101 acctgatcga cgccgggatt cgcatggcgg attgccgggc gctggttatc ctcaccgtga
     5161 acgaggagat caacacgcgc gtcgcgacca tggcgcggct gctcaacccc gagttgcaca
     5221 ttctgtgccg ctcgagttcg cggcgccaca tcgagcagct caaatcgctc ggcaacgtca
     5281 ccatcatcaa tccgttcgaa atcttcgcgc aactgctgag catggcgatt accgcaccgc
     5341 gcctgcacaa tctcaacagc tgcctggtgc gcaacccgat ggcacggctc ggcgaaccga
     5401 tcgaggtgcc gaccggcgac tggatcgtct gcggttacgg ccgcatgggc cgctggatgt
     5461 gccggcattt cgtcgacaac ggcatcaagc cggtgatcat cgatcccgag gccaaacatt
     5521 tggagggcgc ggcacggatt atcaacgatc acgccaaccg cgagtcgctg caggccgccg
     5581 gcatcgatca cgcggccggc gtggtcgccg gcaccgacag cgatcatgtc aacctgaata
     5641 tcctgatgag cgtcaaggca ttgaaacccg atgccttcac catcgtgcgg caaaacagcc
     5701 acgagaacca gatcgccttc gatgccgcgc gcgccgacct ggtcctgcag tccagcctga
     5761 ccaccgcgcg gcgcgtgctc aagcacctga tctcgccgca ggttcagcac ttcgtcgaat
     5821 acctgcgcga gcagggtgaa gacatttgcc agcaggcggt ggagcgcctc aaatcgatga
     5881 tcggcgaccg gccgctacac ctctggcagg tcactctgcg ctcgtcgaag gccgaagccg
     5941 tgatcgagca cctggcagcg aagcgctcgc tgacactcgg cgagttgcgg cgcgatccgc
     6001 gcgatctcga tgcttcgctg ccgtgcatgc cgctatcgat cgagcgcggc gagcggcgca
     6061 tcatgctgcc cgccgacgac gagccgctgc gggtcggaga caaactgctg ttctgcggca
     6121 c
//
LOCUS       DATCZV010000635         4523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_475967, whole genome shotgun sequence.
ACCESSION   DATCZV010000635 DATCZV010000000
VERSION     DATCZV010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475967"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="glyQ"
                     /locus_tag="VIW27_14200"
     CDS             complement(<1..881)
                     /gene="glyQ"
                     /locus_tag="VIW27_14200"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY5740877.1"
                     /translation="MSVTPAIDVNSFQGLIQALQGYWSAQGCLIMQPLDMEVGAGTFH
                     PATFIRAIGPEPWRSAYVQPSRRPTDGRYGENPNRLQHYFQFQVLLKPSPDNFQELYL
                     GSLEALGFDPLVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECH
                     PVSGEITYGIERLAMYLQGVDSTYDLVWSRGPQGVVTYGDIYHQNEVEQSTYNFEIAD
                     TGALFDWFDVCEAQCRVLIDASLPLPAYEQVMKASHAFNLLDARHAISVTERQRFILR
                     VRKLSRAVAESYHASREA"
     gene            986..1504
                     /locus_tag="VIW27_14205"
     CDS             986..1504
                     /locus_tag="VIW27_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5740878.1"
                     /translation="MRNILGHWLVSLRHYFWMCLLLSSPERLPYNPYAFALTVFAYFA
                     IGGLLVDNANDYGVIAARILLEMAMLAAIVRVGLNIRQWPQRFAQTFSALVGINLLVT
                     AVSIPLQALVGVDIEQPSALALYLFVVILVWNLAAISLVFKRAFEISTPLSAMLSFSY
                     FVVNYIIVVLFV"
     gene            1504..2841
                     /gene="mpl"
                     /locus_tag="VIW27_14210"
     CDS             1504..2841
                     /gene="mpl"
                     /locus_tag="VIW27_14210"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY5740879.1"
                     /translation="MKIYILGICGTFMAGIAQIARELGCAVEGSDAQVYPPMSTLLEQ
                     AGISLHEGYDAAALPADCDMFVVGNAITRGNPQFEAIIERGLPYTSGPQWLADNLLRK
                     RWVLAIAGTHGKTTTSSMLAWILQQAGLEPGFLIGGIGQNFPVSARLGSDPFFVIEAD
                     EYDTALFDKRSKFIHYHARTLILNNLEFDHADIFDDLKSIQRQFHHLVRTLPASAQIV
                     VNADDEALAEVLQMGCWSERVSFSCRDPEADFFLDDGPLLRQGAREYPLELPQAGLFN
                     ALNATAALIAARHAGVGIEHSLAALRDFGGVRRRQEIIAEIGGIRVIDDFAHHPTAIK
                     ATLEALRGQTPGQLIGVLDIRSNTMKMGVHGDRLAASFAAADRILLCENPQISWDIRK
                     MLKTAPTIVDIKRDTQQIIEDLKQHSRPGDQIVIMSNGGFEGLHCRLVDALQN"
     gene            2851..4161
                     /locus_tag="VIW27_14215"
     CDS             2851..4161
                     /locus_tag="VIW27_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5740880.1"
                     /translation="MSDRALKLTKIAKYEIKRRIGRGAMGVVYEAFDPFVQRTVAIKV
                     MHSAGNQDPATEQKMREAFFAEVYSAGRMHHPSVISVYDAGQEGDLNYIVMEFVDGMT
                     LQEYVIGDRSLTPNQVVDVIYQCAKGLDYVHREGIIHRDLKPGNIMLSNDGAVKIMDF
                     SIAHVDVGFDGHDTEVQGSPMYMPPEQLSEEKRLVAQSDIYSLGAVMYALLARRPPYK
                     ANSLESLIYKISNLDPEPITEINPEVPKHIVDIVEKAMQKIIYNRYESAYLLARDLSN
                     SFGRLRGVGERIDMQEKWKTLRFLAFFKDFSDDQITEVVNASEWKEFAKGEVIVTEGE
                     QETSFYIIAKGGVEVVKDGRVVGIMKQGDCFGEIAFLTHQPRNATILARTDASLMMVS
                     VTLMEQASTETQLRYYRIFLENLIARLSQATDKLVEADITLAGT"
     gene            4158..>4523
                     /locus_tag="VIW27_14220"
     CDS             4158..>4523
                     /locus_tag="VIW27_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY5740881.1"
                     /translation="MKPGTLTLAMTGASGAEYTLRLFGYCLQQGIHVQFITSQPGQIV
                     LGMETELKLSGPPQKMQQQLADHFAADPRLISVYSKEQWTAPPASGSSVADAMVVCPC
                     SMGTLASIAVGSSDNLIHRA"
BASE COUNT          918 a         1395 c         1336 g          874 t
ORIGIN      
        1 gcttcgcgcg aagcgtggta actctcggcc acggcacgcg acagcttgcg tacgcgcagg
       61 atgaaacgct ggcgttcggt caccgaaatc gcgtggcgcg cgtcgagcag gttgaacgcg
      121 tgcgaggcct tcatgacctg ctcgtaggcc ggcagcggca ggctggcatc gatcagcacg
      181 cggcattgcg cttcgcatac atcgaaccag tcgaacaacg cgccggtatc ggcaatttcg
      241 aagttgtagg tcgactgctc gacctcgttc tggtgataga tgtcgccgta ggtcacgacg
      301 ccctgcggac ctcggctcca gaccaggtca taggtgctgt cgacgccctg cagatacatc
      361 gccaggcgct cgatgccgta ggtgatttcg cccgataccg ggtggcactc gagaccgccg
      421 acctgctgaa aatatgtgaa ctgcgtgact tccataccgt tcagccagac ttcccagccc
      481 agtccccagg cgcccagcgt cggcgattcc caattgtcct cgacgaaccg aatgtcgtgc
      541 accagcgggt cgaagccgag cgcttcgagc gaacccaggt agagttcctg gaaattatcc
      601 ggcgacggct tgagcaacac ctggaactgg aagtaatgct gcagacggtt gggattttcg
      661 ccgtaacggc cgtcggtggg acgccggctc ggttgcacgt aggcgctgcg ccagggctcg
      721 ggaccgatgg cgcgaatgaa ggtcgccgga tggaaagtgc cggcgccgac ctccatgtcc
      781 agcggctgca tgatcaggca gccctgcgcg gaccagtaac cttgcaaggc ctggatcaga
      841 ccctggaaac tgttgacgtc gatggccggc gtaacgctca ttttttcagc aagtcaaaag
      901 ctggtgatta taaccaactg cgattcgtta ccgatacctt ttcctcgccc ttgtgttggc
      961 gctttaaccg gctatgatgt cgcgcatgcg aaacattctc ggccactggc tggtcagtct
     1021 tcggcactac ttctggatgt gcctgctgct gtcgagtccc gagcgcctgc cctacaaccc
     1081 ttatgccttt gcgctgaccg tattcgccta tttcgcgatc ggcggtctgc tggtcgacaa
     1141 cgccaacgat tacggtgtca tcgccgctcg catcctgctg gaaatggcga tgctggcggc
     1201 gatcgtgcgc gtcggcctta acatcaggca atggccgcaa cgctttgcgc aaaccttctc
     1261 cgcgctggtc ggcatcaacc tgctggtgac cgcggtgtcg attccgctgc aagcgctggt
     1321 cggcgtcgat atcgagcaac cctcggcgct ggcgctgtac cttttcgtcg tcatcctggt
     1381 gtggaacctg gcggccatat cgctggtatt caaacgcgct ttcgaaatct cgacgccgct
     1441 ttcggctatg ctatctttta gctatttcgt ggtgaactac atcatcgtgg tcctgttcgt
     1501 ctagtgaaga tatacattct cgggatatgc ggcaccttca tggccggtat cgcgcagatc
     1561 gcgcgcgaac tcggctgcgc ggtcgagggt tccgacgcac aggtctatcc gccgatgagc
     1621 acgctgctcg agcaggccgg catctcgctg cacgagggtt acgacgccgc ggcgctgccc
     1681 gcggattgcg acatgttcgt ggtcggcaac gcgatcacgc gcggtaatcc gcagttcgag
     1741 gctattatcg agcgcggcct gccctacact tccggtccgc agtggctggc ggataacctg
     1801 ctgcgcaagc gctgggtact ggcgatcgcc ggtacccacg gcaagaccac gaccagcagc
     1861 atgctggcgt ggatactgca gcaagccggt ctcgagcccg gtttcctgat cggcggcatc
     1921 ggtcaaaatt tcccggtttc cgcgcgcctc ggcagtgatc cgtttttcgt catcgaggcc
     1981 gacgagtacg acaccgcgct gttcgacaag cgttccaagt tcattcacta ccacgcgcgc
     2041 acgctgatcc tcaacaacct cgaattcgac catgccgata tcttcgacga tctgaaatcg
     2101 attcagcgcc agtttcatca cctggtgcgc acgctgccgg cgagcgcgca aatcgtcgtc
     2161 aatgccgacg acgaagcgct tgccgaagta ctgcagatgg gctgctggag cgagcgggtg
     2221 agcttttcgt gccgcgaccc cgaggccgac tttttcctcg acgacgggcc gctgctgcgg
     2281 caaggcgcgc gggagtaccc gctggaactg ccgcaagcag ggctattcaa cgcgctgaat
     2341 gcgaccgcgg cactgatcgc cgcgcgtcac gccggcgtcg ggatcgagca ttcgctggcc
     2401 gcgctgcgcg atttcggcgg ggtgcggcgg cgccaggaga tcatcgccga aatcggcgga
     2461 atccgcgtaa tcgacgactt cgcgcaccac cccacggcca tcaaagctac cctcgaggca
     2521 ctgcgcgggc aaacccccgg acaactgatc ggcgtgctcg atatccgctc gaacacgatg
     2581 aaaatgggtg ttcatggcga tcggctggcg gcgtctttcg ccgccgcaga ccgcattttg
     2641 ctgtgcgaaa atccgcaaat aagctgggat attcggaaaa tgctgaaaac agcgcctaca
     2701 atagtagata tcaagcgcga tacgcagcag atcatcgagg atttgaagca gcatagtcgt
     2761 cccggcgacc agatcgtcat catgagcaac ggcggctttg aaggtcttca ttgtcgcctc
     2821 gtggacgcgc tacaaaacta gatttcgagt atgtcagaca gagccctaaa acttaccaag
     2881 atcgccaagt acgagatcaa gcgccgcatc ggccgcggtg cgatgggtgt cgtctacgaa
     2941 gccttcgatc cattcgtaca gcgcacggtg gccatcaagg tcatgcactc ggcgggtaac
     3001 caggatccgg cgaccgagca gaaaatgcgc gaagcgtttt tcgccgaggt ctacagcgcc
     3061 gggcgcatgc atcacccgtc ggtaatcagc gtctacgatg ccgggcagga aggcgacctg
     3121 aattacatcg tgatggaatt cgtcgacggc atgacattgc aggaatacgt catcggcgac
     3181 cgcagcctga cgcccaacca ggtggtcgat gtcatctacc aatgcgccaa gggcctcgat
     3241 tacgtgcacc gcgaaggcat cattcaccgc gacctgaagc ccggcaacat catgctcagc
     3301 aatgacggcg cggtaaagat catggatttc agcatcgccc atgtcgacgt cggtttcgac
     3361 ggtcacgaca ccgaggtgca gggctcgccg atgtacatgc cgccggagca attatcggag
     3421 gaaaaacgcc tggtcgcgca atcggacatc tattcgctgg gcgcggtgat gtacgcgctg
     3481 ctggcgagac gcccgccgta caaggcaaac agcctcgaat cgctgatcta caagatcagc
     3541 aacctcgacc cggagccgat caccgaaatc aatcccgagg tgccgaagca cattgtcgac
     3601 atcgtcgaaa aggcgatgca gaagatcatc tacaaccgct acgagtcggc ctacctgctg
     3661 gcgcgcgacc tgtccaactc gttcggccgc ctgcgcggcg tcggcgagcg catcgacatg
     3721 caggaaaaat ggaaaaccct gcgcttcctc gcctttttca aggatttcag cgacgaccag
     3781 atcaccgagg tggtcaatgc cagcgagtgg aaggaattcg ccaagggcga ggttatcgtc
     3841 accgagggcg aacaggaaac atctttctat atcatcgcta agggcggtgt cgaagtcgtc
     3901 aaggacggcc gcgtggtcgg catcatgaaa cagggcgact gcttcggtga gatcgccttc
     3961 ctgacccacc agccgcgcaa cgcaaccatc ctcgcgcgca ccgacgcgtc gctgatgatg
     4021 gtcagcgtga cgctgatgga gcaggcatcg accgaaaccc agttgcgcta ttaccgtatt
     4081 ttcctcgaga acctgattgc aaggctgtcg caggcgaccg acaagctggt cgaggccgac
     4141 atcaccctgg ccggcacatg aagcccggca ccctgacgct ggctatgacc ggcgcctcgg
     4201 gcgctgaata caccctgcga cttttcggct attgcctgca acagggcatc cacgtccagt
     4261 tcatcacctc gcagcccggc cagatcgtgc tcggcatgga aaccgagctc aaactctccg
     4321 ggccgccgca aaaaatgcaa cagcaactcg ccgaccattt cgccgccgat ccccggttga
     4381 tcagcgtcta ctcgaaggag cagtggaccg cgccgccggc cagcggttcg tcggtggccg
     4441 acgcgatggt ggtctgcccg tgcagcatgg gcacgctggc gtcgatcgcg gtcggcagca
     4501 gcgacaacct gattcatcgt gcc
//
LOCUS       DATCZV010000636         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_264528, whole genome shotgun sequence.
ACCESSION   DATCZV010000636 DATCZV010000000
VERSION     DATCZV010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264528"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VIW27_14225"
     CDS             complement(<1..683)
                     /locus_tag="VIW27_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5740882.1"
                     /translation="MEKVWLKSYPAGIPEEIDYAGYQSVTDVFEQAIERFGGNPCFSN
                     LGTALSYDDMDRYTDQLASYLQSLPGLAKGDRVAVMMPNVLQNPIAIFATLRAGFTVV
                     NTNPLYTARELAHQLKDSGARAIIVMENFCHTLEEVIDDTPIEHVVTTQLGDLLKFPK
                     SLIVNLVVKHVKKMVPAFDLPRAVSFKQALALGSRQPHRRVECSHEDLAFLQYTGGTT
                     GVAKGAMLT"
     gene            complement(763..>2409)
                     /locus_tag="VIW27_14230"
     CDS             complement(763..>2409)
                     /locus_tag="VIW27_14230"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5740883.1"
                     /translation="PGIDIGRRHFPLNAAFQNGPNSGKDVFIPIDYVIGGEANIGKGW
                     RMLVESLSVGRGISLPAVGVAAGKACARTTGAYARVRKQFNTSIGRFEGVEEALARIG
                     GLAYMMESGRLLTLSGLDSGEKPSVVTAILKCFNTEFMRRVVNDSMDVHGGRGICMGP
                     SNYIARAYQTLPVGITVEGANILTRSMIIFGQGAIRCHPYLVREMQAATMEDGDAAEF
                     EFDRALRGHAEYFVANFCRAVIYGLSGSHAAPAPDLERIDTYYRRLAQLSAAFAVTAD
                     LALMILGGAFKRKEMMSGRFADAFSYMFYASAALRKFEADGQPRVDLPLVEWSVKYSL
                     YQVQMALDEILRNFPLKWLGVLLRPLIFPLGLSLRPPNDMLCHRVASLLIRPGEARDR
                     LTAGIYLSDDPQDITGCLEDALSRVIAAEPLERRLRHEQLEREGLEDPATWVARLRAA
                     GQIDADEAELLLQSQAATRRVIMVDDFDTAELTSGGQPAKNSGSRKSTGSGRTAGSNK
                     SASGAVAGSKKKAARARRSANNKKQAAGKTDTTTGGAASE"
BASE COUNT          429 a          767 c          739 g          474 t
ORIGIN      
        1 tgggtcagca tggcgccctt ggcgacaccg gtggtaccgc cggtatactg cagaaaggcg
       61 aggtcctcgt ggctgcattc cacccgccgg tggggctgcc gtgatcccag cgccagggcc
      121 tgcttgaagc tcacggctcg cggcaggtcg aaggccggca ccatcttctt gacgtgcttg
      181 accaccaggt tgacgatcag cgacttcgga aacttgagca ggtcgcccag ctgcgtggta
      241 accacgtgct cgatcggcgt atcgtcaatc acttcctcga gcgtgtggca aaaattttcc
      301 atcacgatga tggcccgggc accggagtcc ttgagctggt gcgcgagttc gcgcgcggta
      361 tacagcggat tggtattgac caccgtaaac ccggcccgca gcgtggcgaa aatcgcaatc
      421 ggattctgca gcacgttggg catcatcacc gcgacccggt cgcccttggc cagtccgggc
      481 aggctttgca ggtagctggc cagctggtcg gtataacgat ccatgtcgtc gtagctgaga
      541 gccgtgccaa gattgctgaa acaggggttg ccgccgaaac gctcgatcgc ctgctcgaag
      601 acgtcggtga ccgactggta accagcgtaa tcgatctcct ccggaatccc ggcgggatag
      661 ctttttaacc agaccttttc catcgccttt tctccccagc cgaagacagc tgatcgcggt
      721 gccaccataa ctcgagcggg cgcggttagc gcgcggccgc cgctattcgg atgcggctcc
      781 gcccgtcgtg gtgtctgttt tcccggctgc ttgttttttg ttgtttgccg accgcctcgc
      841 gcgcgcggct ttctttttac ttccggcgac ggcgccactg gcgcttttgt tgctgcccgc
      901 ggtcctgccg ctaccggtac ttttcctgct gccgctattt ttcgccggtt gacccccact
      961 tgtcaattcc gcggtatcga aatcgtcaac catgatcact cgccgggtcg ccgcctgcga
     1021 ctgcagtaac aattccgctt cgtcggcgtc gatctggccg gcggcgcgca acctggcaac
     1081 ccaggtggcg ggatcctcca gcccttcccg ctcgagttgc tcgtggcgca ggcgccgctc
     1141 gagtggctcg gcggcgatca cccggctcag cgcgtcctcg agacaaccgg taatatcctg
     1201 cggatcgtca ctgagataaa taccggcggt caggcggtcg cgcgcttcgc cggggcgaat
     1261 caacagcgac gcgacgcggt gacagagcat gtcgttgggt ggcctcaggc tcaggcccag
     1321 cgggaaaatc agcggccgca gcagcacgcc gagccacttc agcggaaagt tgcgcagaat
     1381 ttcgtcgagc gccatctgca cctggtaaag cgagtacttc actgaccatt cgaccagcgg
     1441 caggtcgacg cgcggctggc cgtcggcctc gaacttgcgc aacgcggccg aggcataaaa
     1501 catgtaggaa aaggcatcgg cgaagcgtcc cgacatcatc tccttgcgct tgaaagcgcc
     1561 gccgagaatc atcagtgcca ggtcggcggt taccgcaaac gcggcgctga gttgcgcgag
     1621 gcgccggtaa taggtatcga tacgctcgag gtccggtgcc ggtgcggcat ggctgccgct
     1681 caatccgtag atcaccgcgc ggcagaaatt ggcgacgaag tactcggcat ggccgcgcaa
     1741 tgctcgatcg aattcgaatt ccgcggcgtc gccgtcttcc atggtcgcgg cctgcatctc
     1801 gcgcaccagg tacggatggc agcgaattgc accctgaccg aagatgatca tgctgcgggt
     1861 cagtatgttg gcaccctcga cagtgatgcc aaccggcagt gtctggtagg ccctggcgat
     1921 gtaattcgac ggtcccatgc atattccacg gccgccgtgc acgtccatcg agtcattgac
     1981 gacccggcgc atgaattcgg tattgaagca tttcaggatc gcggtaacca ccgacggttt
     2041 ttcgccgctg tcgagaccgg acagggtcag caggcgcccg gactccatca tgtaggcgag
     2101 tccgccgatg cgcgccaggg cttcctcgac accctcgaag cggccgatcg aggtattgaa
     2161 ttgcttgcgc acccgcgcat aggcgccggt ggtgcgcgca caggccttgc cagcggccac
     2221 gcctaccgcc ggcagcgaaa tgccgcggcc gaccgacagc gactcgacca gcatgcgcca
     2281 gcccttgccg atattcgctt cgccgccgat cacgtaatcg atcggaatga atacatcctt
     2341 gccgctgttg ggcccgttct ggaaagccgc gttgagcgga aaatggcgcc tgccgatgtc
     2401 gatcccggg
//
LOCUS       DATCZV010000637         5013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_264555, whole genome shotgun sequence.
ACCESSION   DATCZV010000637 DATCZV010000000
VERSION     DATCZV010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264555"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VIW27_14235"
     CDS             complement(<1..1515)
                     /locus_tag="VIW27_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5740884.1"
                     /translation="MKSHARVVIIGGGSLGVNLLYHLTREGWSDVVLVEKGELTSGST
                     WHAAGLCGNFIGNHTVSHIHNYTVRLYNEILPEETGEPSVFHQTGSIRQGFTRVEEEW
                     FRNLESRARNIGFEFNIVSKAEAQKLNPFMNFDKARVIASTPNDGHVDPTSVVMPLAR
                     LARNNGAEIYRNNRVLEINEQRNGEWQVVTEQGTITAEHVVNAAGCFAREIGAMVGIN
                     VPLINLEHQYLVTESHPDIEALDFELPVCRDSWSSAYIRQEGNGFLIGPYEMWGSRPW
                     ALDGMDWNFDRGLFEWNLEAIMPFLERCYELTPRVEDVGVKTVINGPITHTPDDNILA
                     GPVAGLRNFWNLCGASIGIAQGGVGKFVAQWMKYGQTEINMAPLDTRRFGDWAAKKYC
                     IVKAIESYEAMYAVAAPNDNRPHGRPLRTSPLYARLLEHGAVHGAAGGFEKPLWFHTD
                     TVRSETLSWSHSEAHPVVGEECRAVQQAAGVIDISGSAKYEISGADATAFLEHLS"
     gene            complement(1520..3100)
                     /locus_tag="VIW27_14240"
     CDS             complement(1520..3100)
                     /locus_tag="VIW27_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5740885.1"
                     /translation="MARRSNAREAKIALRKAALAEELKPVHPGESGGQYKPLKDTDIE
                     QIEATVYRILEEVGFADANQHCIETCLAVGATYGEDRRLRFPRAVVDEALARCRRDLV
                     LHGQDSKHDLQLSGSRVHFSTAGAAVMIADPVNNEYRETRAQDLYDMARIADNCEHVH
                     MFQRTCVLRDVTDNRDMDINSAYNSIMGTTKHIGCSWTEASHLEDTLKMLHLVAGGED
                     KWRERPFMSISCCHVVPPMKFTDEALSCIKVAVEGGMPVLLLSAGQSGATAPPCLAGA
                     VAQAWAECLGGLVFVNAIKPGAPAILGTWPFVSDLRTGAMSGGSPEQAILSAACAQMG
                     NYFNLPTGTACGMTDAKLPDFQAGAERAYTLACAATAGANIVYESVGMYASLLGVCPE
                     SMLMDNDVLGAALRITRGIEVNEDTLGFEVIKDVCLGNKGHYLGAGQTLEVMQTEYLY
                     PDLGDRLSPNEWAEQGKPVLLDKALARKQEILGNYFPGHVSDEVDRQIRQQFNIYLPR
                     EAFGRLSSGDDDAQLQHG"
     gene            3221..4102
                     /gene="gcvA"
                     /locus_tag="VIW27_14245"
     CDS             3221..4102
                     /gene="gcvA"
                     /locus_tag="VIW27_14245"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEY5740886.1"
                     /translation="MGRRLPPLNSLKCFEAAGRLLSFTAAARELNVTQAAISHQIKVI
                     EDYLGVSLFDRYPRRLALTEQGKTLLPEVTEAFDRVSSVIGELGEEQYSRTINVRLGP
                     SFAARWLSPRLKYFWQQHPDIDLCLFHSNAATDFEREQMDIAVTYGKGSWPGLVADKL
                     LSLDFFPVCSPSFLDNDKPLTKLANLRYYTLLHDASHECWHDWLDLAEVGEVRPEKGV
                     IIDDTNVLIQAAIDGQGIALGSSAFIQDLLDSGKLIKPFDIELVNEYAYYVVCPQSHL
                     KNPAVQAFKEWLLSLVD"
     gene            4139..4711
                     /locus_tag="VIW27_14250"
     CDS             4139..4711
                     /locus_tag="VIW27_14250"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY5740887.1"
                     /translation="MQSHDELRQRNRKLRAELDDAALATAAAALAARVVTLPEFIAAK
                     RVAAYAAINGEIDLRPVIERALADGKSVYLPNLDRESLRFSPYFHGQKMRVNRFKLLE
                     PDVGDEEMLQPQDLDLVLAPLVVFDVERNRIGMGGGYYDRSFAFRKDPRRTTPLLIGV
                     AHELQKVAHIEPESWDVRLDMIVTDAAIYQ"
BASE COUNT          899 a         1555 c         1559 g         1000 t
ORIGIN      
        1 ggacaggtgc tcgaggaaag cggtggcgtc ggcgccgctg atctcgtact tggccgagcc
       61 ggaaatgtcg atcacaccgg cggcctgctg cactgcccgg cattcctcgc caaccaccgg
      121 atgcgcctcg ctgtgcgacc agctcagagt ttcgctgcgt accgtgtcgg tgtggaacca
      181 gagcggcttc tcgaagcccc cggcggcacc gtgcaccgcg ccgtgttcga gcagccgggc
      241 gtagagcggg ctggtgcgaa gcggtcgccc gtgcgggcgg ttgtcgttcg gcgcggcgac
      301 cgcgtacatc gcctcgtaag actcgatcgc cttgacgatg cagtatttct ttgccgccca
      361 gtcgccgaaa cggcgcgtgt cgagcggtgc catgttgatc tcggtctggc cgtatttcat
      421 ccattgcgcg acgaacttgc cgacgccgcc ctgggcgatg ccgatgctgg cgccgcacag
      481 gttccagaaa ttgcgcagcc cggcgaccgg cccggcgaga atattgtcgt ccggcgtgtg
      541 cgtgatcggg ccgttgatga ccgtcttgac accgacatcc tcgacccgcg gcgtaagttc
      601 gtaacagcgc tcgagaaacg gcatgatcgc ctccaggttc cattcgaaca ggccgcggtc
      661 aaaattccag tccatgccgt cgagcgccca gggccgcgaa ccccacattt cgtagggacc
      721 gatcaggaag ccgttgccct cctggcggat atacgccgaa ctccacgaat cccggcatac
      781 cggcagctcg aagtctagcg cctcgatgtc ggggtggctc tcggtgacca ggtactggtg
      841 ctcgaggttg atcagcggca cgttgatgcc caccatggca ccgatttcgc gcgcgaagca
      901 gccggccgcg ttgaccacgt gctcggcggt gatcgtgcct tgctcggtga ccacctgcca
      961 ttcgccgttg cgctgctcgt tgatctcgag cacgcggttg ttgcggtaga tctcggcgcc
     1021 gttgttgcgt gccagcctgg ccagcggcat gaccaccgag gtcgggtcga cgtggccgtc
     1081 gttgggcgta ctggcgatga cccgtgcctt gtcgaagttc atgaacgggt tgagtttttg
     1141 cgcctctgct ttgctgacga tattgaattc gaaaccgata ttgcgggcgc ggctttcgag
     1201 attacggaac cactcctcct cgacgcgcgt gaagccctgg cgaatactgc cggtctggtg
     1261 gaacacggac ggctcgccgg tctcctcggg tagaatctcg ttgtacagcc tgaccgtgta
     1321 attgtggata tgcgagaccg tgtgattgcc gatgaaattg ccgcacaggc ccgctgcatg
     1381 ccaggtagag ccgctggtca gttcgccttt ttcgaccagc accacgtcgc tccagccttc
     1441 cctggtcagg tggtagagca ggttcacacc cagtgaacct cccccgatga tgactaccct
     1501 ggcgtgactt ttcattgctt tacccgtgtt gaagctgcgc gtcgtcatca ccactcgaca
     1561 ggcggccgaa cgcctcgcgc ggcaaataaa tgttgaattg ttggcgaatc tgccggtcga
     1621 cctcgtcgct gacgtgaccc gggaagtaat tgccgaggat ctcctgcttg cgcgccagcg
     1681 ccttgtcgag cagcaccggc ttgccctgtt cggcccactc gttggggctc aggcggtcgc
     1741 cgagatccgg gtacaggtac tcggtctgca tgacttcgag ggtctgcccg gcgccgaggt
     1801 aatggccctt gttaccgagg caaacgtcct tgatgacttc gaagccgagg gtatcctcgt
     1861 tgacctcgat gccgcgtgtg attctgagtg cggcgccgag cacgtcgttg tccatcagca
     1921 tcgattcggg gcagacaccg agcaggctgg cgtacatgcc gaccgactcg tagacaatat
     1981 tggcgccggc ggtcgccgcg caggccagcg tgtaggcgcg ctcggcgccg gcctggaaat
     2041 ccggcagctt ggcatcggtc atgccgcagg cggtgccggt cggcaggttg aaatagttac
     2101 ccatctgcgc gcaggccgct gacaggatcg cctgctctgg cgaaccgccg ctcatcgccc
     2161 cggtacgcag gtcggagacg aacggccagg tgccgagtat ggccggcgcg ccgggtttga
     2221 tggcgttgac gaataccagg ccgccgagac actcggccca ggcctgtgcc accgcgcccg
     2281 ccaggcaggg cggtgcggtc gcgccggatt gtcccgccga cagcagcagc accggcatgc
     2341 cgccctcgac cgcgaccttg atacaggaga gcgcttcgtc ggtgaacttc atcggcggca
     2401 ccacgtgaca gcaggagatg ctcatgaacg gccgctcgcg ccacttgtcc tcgccgccgg
     2461 ctaccaggtg cagcatcttc agtgtgtcct cgagatggga agcctcggtc cagctgcagc
     2521 cgatgtgctt ggtcgttccc atgatcgagt tgtaggcgga attgatatcc atgtcgcgat
     2581 tgtcggtcac gtcgcgcagc acgcaggtgc gctggaacat gtgcacgtgc tcgcagttgt
     2641 cggcgatacg cgccatgtcg tagagatcct gggcgcgggt ttcgcgatac tcgttgttca
     2701 ccgggtcggc gatcattacc gcggcaccgg cggtcgagaa atgtacgcgc gagccggaaa
     2761 gctgcaggtc gtgttttgaa tcctggccgt gtagcaccag gtcgcggcga cacctggcga
     2821 gcgcttcgtc gaccaccgcg cgcggaaagc gcaggcgccg gtcctcgccg taggtggcgc
     2881 cgaccgcgag gcaggtttcg atgcagtgct ggttggcgtc ggcgaaaccg acttcctcga
     2941 ggatgcgata aacggtcgcc tcgatctgtt cgatatcggt atcctttagc ggcttgtact
     3001 ggccgccgga ttcgccggga tgcaccggct tcagttcttc cgccaaagcg gccttgcgca
     3061 gcgcgatttt cgcctcgcgg gcgttcgagc gtcttgccat ggtatttctc cggtaacgga
     3121 tgatcggcgg agcacgcgga ttgtcgaacc agaatcgaat ggattcaaac gattattttt
     3181 tatactatgg attagtttaa ataatccata ggtaattctg atgggccgcc gactgccgcc
     3241 gctcaacagt ctcaagtgtt tcgaggccgc cggccgcctg ctgagcttta ccgccgcggc
     3301 gcgtgaactc aacgtcaccc aggccgcgat cagccaccag atcaaggtga tcgaggatta
     3361 cctcggcgta tcgctgttcg accgctaccc gcgccggctg gcgctgaccg aacagggcaa
     3421 gacgttgctg cccgaggtca ccgaggcctt tgaccgggta tcgtcggtga tcggcgaact
     3481 cggcgaggaa cagtactcgc ggacgatcaa cgtgcgcctc ggcccgtcgt tcgcggcacg
     3541 ctggctgtcg ccgcggctca agtatttctg gcagcagcac ccggacatcg acctgtgcct
     3601 gtttcactcc aacgcggcga ccgatttcga gcgcgaacaa atggatatcg cggtgaccta
     3661 cggcaagggc agctggccgg ggctggtcgc ggataaattg ctgagcctcg attttttccc
     3721 ggtctgttcg ccgtctttcc tcgacaacga caagccgttg accaaactcg ccaacctgcg
     3781 ttattacacc ctgctgcatg acgccagcca cgagtgctgg cacgactggc tcgatctcgc
     3841 cgaggtcggc gaggtgcgcc cggagaaggg cgtcatcatc gacgatacca acgtgctgat
     3901 ccaggccgcg atcgacggcc agggcatcgc gctcggttcg tcggcgttca ttcaggacct
     3961 gctcgattcc ggcaagttga tcaagccctt cgacatcgag ctggtcaacg agtatgccta
     4021 ctacgtcgtc tgtccccaat cgcacctcaa gaacccggcg gtacaggcct tcaaggagtg
     4081 gctactcagc ctcgtcgatt gacagtatca tgccggcatc ccgaagccgg agggtgatat
     4141 gcaatcccac gacgaactca ggcagcgcaa ccgcaagctg cgtgcggaac tcgacgatgc
     4201 agcgctggcc accgccgcgg cggcgctcgc cgcgcgcgtc gtcaccttac ccgaattcat
     4261 cgccgcgaaa cgcgtggccg cctacgccgc gatcaacggc gaaatcgacc ttaggccggt
     4321 catcgagcgt gcgctggccg acggcaaaag cgtctacctg ccgaaccttg accgcgaatc
     4381 gctgcgcttt tcgccgtatt tccacggcca gaagatgcgc gtcaaccgtt tcaagctact
     4441 ggaaccggac gtcggcgacg aagagatgct gcagccgcaa gacctcgacc tggtgctcgc
     4501 gccgctggtg gttttcgacg tcgagcgcaa ccgcatcggt atgggcggtg gctactacga
     4561 tcgcagcttt gcgttcagaa aagatccgcg gcggacaacg ccgctactga tcggcgtcgc
     4621 ccacgaactg caaaaagtcg cgcatatcga acccgaatcc tgggacgtgc gcctcgacat
     4681 gattgttact gacgccgcca tttatcaata ggcagcggaa tcccatagcc gttcagtttg
     4741 atttgaatcg gggccagcgg atgttccatc cctctggtcg attggatatc gctatacagg
     4801 aagaaactgg tattcttggc gaatcggctg cagccaggtt gtacctggcc gaaactcgat
     4861 cacccgattg ccgcgggcct tgaacgtcaa caggagtaat gcccggaggg tgtctgtatt
     4921 tgaaaaatga aacctatggc ggcgaagtaa tcgtgcggcg cgtcactatg ttaccggtat
     4981 gcgctgagcc ggagacagca gcgaacgagc ccc
//
LOCUS       DATCZV010000638         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_423235, whole genome shotgun sequence.
ACCESSION   DATCZV010000638 DATCZV010000000
VERSION     DATCZV010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423235"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..867
                     /locus_tag="VIW27_14255"
     CDS             118..867
                     /locus_tag="VIW27_14255"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5740888.1"
                     /translation="MSEFSGKTVLVTGAGRGIGRQIALEFAAAGANLCVNYAHSAREA
                     QRVVEAIEQNGGRALACEADIADAAAVAAMVERAMQAFGAIDVLVNNAGLSRGGPFLQ
                     ISEADWDDVYAVNLKGPFLVSQAVARRMLDGDGGVIVNISATTTILGRANAADYCATK
                     AGLNMLTKCMALELGPKVRVNGIGLGFVDSPLVREVFSAEQLQEVLDTTPLKRMTTMQ
                     ETAAFVRLLASDAASFVTGQTVMFDGGRVMR"
     gene            1009..1794
                     /locus_tag="VIW27_14260"
     CDS             1009..1794
                     /locus_tag="VIW27_14260"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY5740889.1"
                     /translation="MPKSTARHRIRCHALALSLCLPASTSAPLAASDWSVGLGAGVIS
                     APEYLGGDSIETFAVPAIDIAYRRTITFNFYEGLNAYFFNRDAISLKAGVAWQPGRDE
                     DEDPSLAGIGDIDDATLLRLVAEYQFDIYTAFFGLRRHYGGTDGTQFEAGIQAFYTLR
                     EEITSPRLLFNLAVLYSDDDYMQGYFGIDAGQAAASGLQPYSAESGISSLRAGATAFY
                     PLTRNWRLTGVLQYRELVGDAGDSPLVLQDGQWFGGAFVGYRF"
     gene            complement(1799..>2095)
                     /locus_tag="VIW27_14265"
     CDS             complement(1799..>2095)
                     /locus_tag="VIW27_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEY5740890.1"
                     /translation="SVMSNARDGFAVCDAGLKVMSLDSGLPVIDGRDGVEYVNCSDEH
                     GVIADPRNSLQLNDKLKLIPGHCDPTCNLHDWYVGIRDRRVECLWPVSARGKSL"
BASE COUNT          348 a          679 c          676 g          392 t
ORIGIN      
        1 gcgatccggt tgccgcgccg gcgcgagccc tgcccctcgt cggccgcggc atcgccgacg
       61 attagtgcgc ccgccggtaa tcgtttatga tcgtcgccaa agcggaggaa tatcgaggtg
      121 tccgaattca gcggcaagac cgtgctggtc accggtgccg gccgcggcat cggccggcag
      181 atcgcactcg agtttgccgc cgcgggtgcc aacctgtgcg tcaattacgc gcactcggcg
      241 cgcgaggcgc agcgcgtggt cgaagccatc gagcaaaacg gcggcagagc gcttgcctgc
      301 gaggccgaca ttgccgatgc ggcggcggtc gcggcgatgg tcgagcgcgc gatgcaggca
      361 ttcggtgcca tcgacgtgct ggtcaacaat gccggcctca gccgcggcgg accgtttctg
      421 cagatttcgg aagcggactg ggacgacgtc tacgcggtca acctgaaggg cccgttcctg
      481 gtctcgcagg ccgtcgcgcg gcgaatgctc gacggcgacg gtggcgtgat cgtcaacatc
      541 tcggctacca ccaccatcct cggcagggcc aatgccgccg actattgcgc gaccaaggcc
      601 ggcctcaaca tgctgaccaa gtgcatggcg ctcgagctcg gcccgaaagt gcgcgtcaat
      661 ggcatcggtc tcggtttcgt cgattcgccg ctggtacgcg aggtattcag cgccgaacag
      721 ttgcaggaag tgctcgacac cacgccgcta aagcgcatga ccacgatgca ggaaaccgcg
      781 gccttcgtca ggctgctggc ctccgatgcc gccagcttcg tcaccgggca aaccgtgatg
      841 ttcgacggtg gccgggtgat gcgctagcgc cagcgggccg acttcggcgg cgacggcgat
      901 cgattcgcca cgtcttcgct ggctgctgat tttcctgccg ccgtcagcga gggtgatttg
      961 tgggcgcggt ttgtctagta ttccggcttt tgcgagccgc tttcactgat gccgaaatcc
     1021 accgcccgcc atcgaattcg ctgccatgcc ctggcgttat cgctttgcct gccggcctcg
     1081 acgtcggcgc cgctcgcggc cagcgactgg agcgtcgggc tcggcgcggg ggtgatctcg
     1141 gcgcccgagt atctcggtgg cgacagtatc gaaacctttg cggtaccggc aatcgacatc
     1201 gcctaccggc gcacgattac cttcaatttt tacgaggggc tgaacgcgta ttttttcaac
     1261 cgcgatgcca tcagccttaa ggccggcgtc gcctggcaac ccgggcgcga tgaagacgaa
     1321 gacccttcac tggctggcat cggcgatatc gacgacgcca ccctgctcag gctggtcgcc
     1381 gaataccagt tcgacattta cacggctttt ttcggcttgc gccggcacta tggcggcacc
     1441 gacggtaccc agttcgaagc cgggatccag gccttttata cgctgcgcga ggaaatcacc
     1501 agcccgcgcc tgctgttcaa cctcgcggtc ctctactccg acgacgatta catgcagggt
     1561 tacttcggca tcgacgccgg gcaggcggcc gccagcggcc tgcagcctta ctcggccgaa
     1621 tcgggaatca gctcgctgcg cgccggcgcg acggcttttt atccgctgac ccggaactgg
     1681 cgcctgaccg gcgtgctgca gtatcgagaa ctggtcggcg acgccggcga cagcccgctg
     1741 gtgttgcagg acgggcagtg gttcggcggc gcttttgtcg gttaccgttt ctgacggttc
     1801 acaaggactt gccgcgcgcg ctgaccggcc acaggcattc gacgcgccga tcgcgaatgc
     1861 cgacgtacca gtcgtgcagg ttgcaggtcg gatcgcagtg accgggaatc aacttcagct
     1921 tgtcgttgag ttgcaatgaa ttgcgcgggt cggcgatcac gccgtgctcg tccgagcagt
     1981 tgacgtattc gacgccgtcg cgaccgtcga tcaccggcag gccggaatcg agcgacatca
     2041 ccttgagccc ggcgtcgcag accgcgaaac cgtcgcgcgc gttggacatc accga
//
LOCUS       DATCZV010000639         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U79
            k141_105731, whole genome shotgun sequence.
ACCESSION   DATCZV010000639 DATCZV010000000
VERSION     DATCZV010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962282
            Sequence Read Archive: SRR25158267
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 19:36:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,827
            CDSs (with protein)               :: 2,827
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 4 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 4 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105731"
                     /isolate="SMAG_U79"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VIW27_14270"
     CDS             complement(<1..796)
                     /locus_tag="VIW27_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5740891.1"
                     /translation="MAGESTIGAIDVWQVRIPLARPYHLSKTYGTLTHSDAALLRLTL
                     ADGTEGWGEADPGGINFDGETIDTVMRGLREHTAGLPGRDLGQQLRDGDQQGLHGAVA
                     AAIDVACHDALARSRGIPVCELLGGRRRDAIDVLWPTSSGSAADDLDVIAEFNPRGFD
                     TYMLKMGDRAVDDDIARAAEVTARLPAGVRVMVDANQGWQRDEALRFAIETRDLPLIL
                     IEQPVAAADLDGLRAVREVAACPLSVDESVQRRADVGAILQAGAADV"
     gene            complement(800..1786)
                     /locus_tag="VIW27_14275"
     CDS             complement(800..1786)
                     /locus_tag="VIW27_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5740892.1"
                     /translation="MLDYILRRFVYTLITIFAVASILFVLFRMLPGEATLQVISPAMD
                     TSVQERMKRAFGLDQPLLIQYFIYLKNLVTLEWGRSFVSAQEVTDILAYRFWNTILLM
                     AAGMCLTLVVGIGFGIIMAWRRNSALDIGGTVVSLIFQAAPPFITGLLLLIFLSYRLN
                     LFPTGGMYPPGQRPDGLLELLMMPAFWERIVLPTITVGAYYIATPMLIMRDSMLEVLG
                     SDFIELAKAKGLRPSVVMFKHAARNALLGVVTISGIMIGFAVGGQVVVESLFSWPGMG
                     QLMVESAASHDYPVAQGTFLMLAILVIGLNFLTDVLYCYLDPRIKITAKGMN"
     gene            complement(1856..>2255)
                     /locus_tag="VIW27_14280"
     CDS             complement(1856..>2255)
                     /locus_tag="VIW27_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5740893.1"
                     /translation="DAMAAAIEAGECDRTRYILKPALVQDLDKVPGVAGRGYASHGMY
                     GFMFNHLRGPFQDRNFREAIDLVIPRDVIREVVMAGFATNGGSVIAPANEFWHNPNVG
                     GRTQNLRRARDLLGKAGYSWDDDGKLLYPA"
BASE COUNT          402 a          733 c          752 g          368 t
ORIGIN      
        1 atacatccgc ggcaccagcc tgcagaatgg cgccgacgtc ggcccgccgc tgcaccgatt
       61 cgtcgaccga cagcgggcag gcggctacct cgcgcaccgc gcgcaggccg tcgaggtcgg
      121 cggcggcgac cggttgctcg atcaggatca gcggcagatc acgggtttcg atcgcgaagc
      181 gcaacgcctc atcgcgttgc cagccctggt tggcgtcgac catcacgcgc accccggccg
      241 gcagtcgtgc cgtaacttcc gcagcccgcg cgatgtcgtc gtcgaccgca cgatcaccca
      301 ttttcagcat gtaggtgtcg aatccgcgcg ggttgaattc ggcgatgacg tcgaggtcgt
      361 cggcggcgct gccgctcgag gtcggccaca atacgtcgat tgcatcgcgc cggcgaccgc
      421 ccaacagctc acagaccgga atgccccggc tgcgcgccag cgcgtcgtga caggcgacgt
      481 cgatggcggc cgcgaccgcg ccgtgcaagc cctgttggtc gccgtcacgc agctgttggc
      541 cgaggtcgcg acccggcagg ccggcggtat gctcgcgcag tccacgcatt accgtgtcga
      601 tggtttcacc gtcgaaattg atgccgccgg ggtccgcttc gccccagccc tcggtgccgt
      661 cggccaaggt caggcgcagc agcgcggcgt cgctatgggt cagcgtgccg taggtcttcg
      721 acaggtggta gggccgcgcc agcggaatgc gcacctgcca gacgtcgatc gcaccgatgg
      781 tcgactcgcc ggccatgatt caattcatgc ccttcgcggt gatcttgatc cgcgggtcga
      841 ggtaacagta gagcacgtcg gtgaggaaat tgagcccgat caccaggatt gccagcatca
      901 gaaaggtacc ctgcgccacc gggtagtcgt gcgaggcggc cgattcgacc atcagctgcc
      961 ccatgccggg ccaagagaac agcgattcga ccaccacctg gccgccgacc gcgaaaccga
     1021 tcatgatgcc ggagatcgtg accacgccga gcagcgcatt gcgcgccgcg tgcttgaaca
     1081 tgaccacgct gggcctcaag cccttggcct tcgccagctc gatgaaatcg ctgcccagca
     1141 cttcgagcat gctgtcgcgc atgatcagca tcggcgtggc gatgtaatag gcgccgacgg
     1201 taatcgtcgg caacacgatg cgctcccaga aggccggcat catcagcagc tcgagcaggc
     1261 cgtcgggtcg ctgcccgggc gggtacatgc cgccggtggg aaacaggttc aagcgatagc
     1321 tgaggaagat cagcaacagc agaccggtga tgaacggcgg tgccgcctgg aatatcaggc
     1381 tgaccacggt gccgccgatg tcgagcgcgc tgttgcgccg ccaggccatg atgatgccga
     1441 agccgatacc gaccaccagc gtcaggcaca tgccggcggc catcagcagg atcgtgttcc
     1501 agaagcggta ggcgaggatg tcggtgactt cctgcgccga cacgaaggag cgcccccact
     1561 ccagcgtgac caggtttttc aggtaaatga aatactggat caacagcggc tgatcgaggc
     1621 cgaaggcgcg cttcatgcgc tcctgcaccg aggtgtccat cgccgggctg atcacctgca
     1681 gggtggcttc gcctggcagc atgcggaaca ggacgaacag gatcgacgcg accgcgaaga
     1741 tcgtgatcag ggtgtagacg aagcggcgca ggatgtaatc gagcatggtc tggttcggtc
     1801 gagcgggcgg caggccggga gtccgtgcgg acgctcggcc gtgccgcgcg ctagcctagg
     1861 ccgggtagag cagcttgccg tcgtcgtccc aactgtagcc ggccttgcca agcaggtcgc
     1921 gcgccctgcg caggttttgc gtgcgcccgc cgacgttcgg gttgtgccag aactcgttgg
     1981 ccggcgcgat caccgagccg ccgttggtgg cgaagcccgc catcaccacc tcgcgaatca
     2041 cgtcgcgtgg aatcaccagg tcgatcgcct cgcggaaatt gcgatcctgg aacggtcccc
     2101 gcaggtggtt gaacatgaag ccgtacatgc cgtggctggc ataaccgcgg ccggcgacgc
     2161 cgggcacctt gtcgaggtcc tgcaccagtg cgggtttcag gatatagcgg gtgcggtcgc
     2221 attcgccggc ctcgatggcg gctgccatcg cgtcg
//
